kif27 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following kif27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa41341 XM_018090329.1
Latest version!
Xenopus tropicalis kinesin family member 27 (kif27), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa49291 XM_031894957.1
Latest version!
Xenopus tropicalis kinesin family member 27 (kif27), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa36894 XM_012955029.2
Latest version!
Xenopus tropicalis kinesin family member 27 (kif27), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa41341
    Clone ID Related Accession (Same CDS sequence) XM_018090329.1
    Accession Version XM_018090329.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF27 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683785.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCTTAT TAGCAGCAGG CAGCGCAGCG CGCCATACAG GCAGCACGCA AATGAACGAG 
    AGATCCAGCC GCTCCCACGC CATCTTCACC ATCACTATAT GCCAACAAAG TTCTCAACAC
    GGAGACGACG CTGGCACAAA CTCCATCCAG ACAATCACTT CCAAGTTTCA CTTTGTTGAT
    CTGGCAGGCT CAGAAAGAGT GACCAAAACT GGGAACACTG GGGAAAGGTT TAAGGAATCG
    ATCCAGATAA ATAGTGGTTT ACTTGCCCTT GGAAACGTGA TAAGTGCACT TGGTGACCCG
    AAAAGAAAAA GTGCACATAT TCCTTATAGG GATGCCAAAA TCACTCGTAT ACTAAAGGAC
    TCCTTAGGTG GAAATGCCAA AACAGTAATG ATTACTTGTA TCAGCCCTTC GTCTTCTGAC
    TTAGATGAAT CACTAAACTC TCTGAAATAC GCCAACCGAG CCAGAAACAT TAAAAACAAA
    CCAGTTGTAA ATTACAATCC TGACTGGGAT AGGATGGATG AAATGGAGCT GGAGATCAAG
    GTTCTAAGAG AAGCTCTGCA AAACCAGCAG AGCAGTAGGG TCAGTCGTAC CAGCCAGGTG
    TCCCAAGACT GGAGCCAAGG AAAAGACAAG ATTCGTTCAC TAGAAGAAAA AGTCGCTCAA
    CTCCAGCTGG AATGTTTCAA CCAACGGCAT TGCACCGAGG AAGCATTCTC ATTGTTCCTT
    GATCTAAAGG ATATTTCTGG CTTACCCCAA TCTGAGAAGA ACAGAGTGCA GGACTGGCTT
    CACTTGGCAG AGGGCCTGAG GAGTCCGGTG GCAGCAGATG CCGAGGGCAG TAGCAGAGAA
    GAGCCACATC ATGTCACCAT CTTGCAGCTG AAGAGGGAAC TGAAGAAATG TCAGGATGCC
    CTAGCAACAG ATGAAGAGGT TTTCGCTAGG AAGAAGCTGG AACTGAAAGG GTTACAAGAG
    CAGATTCAGT CGCTACAGCA AGAAAACGAA GAATATCAGA AGTGCCTGCA AGAGGCAGAA
    TATACTAAGA CTTTGCAGAC TGAAAAACTG GTGGAACAGC AGCTCCTTAT TGATGAATTG
    AAGTCAAAGA AGCCGGAAAT CTACAGGCAG TTTTCGCAGG AAAATGAAGA TGAAGAGGAA
    CTTCCAAGGG AAATATCCCA AAGGCCACAC ACTGTGCCCC CCAGCACCCA CACCCAGAAA
    CTCTCGTCCA GTTCATTGCA GGCTGCAGGG CAGGCAGAGC CAAGGAAGGT ACGAACAAGC
    CCCCCTTCCT ATTCCCTAGA AAGAATGGTA GCGGGATTCC GGACTCGCAG CCAGATGCTC
    CTGGAGCTCA TAGAGGAGCA GGATGAAGTT CTGCATGAGA AGTTCTCAGA CCAAAGTGAA
    GAAGAACCTG ATGAGCCAGA GAAAGAAAAC AGAAAGAAAT CACCATTTAG GCGGTCTATC
    AATCGGACAT GGACACGACG ACAAGGCTCT GCTGGTGCGG AAACACTTAA CTCCAACTCA
    GACAGAGAGA ATGACGCAAA CTCTAATGCT TGTCCAGCTC TGGAATCCCT GCGAGAAAGT
    CAGGTGCTGA ATGTGGAGAA ACTGAAAACC TCAGACGTTC GCCTCGCTGA TGCCAAGCAG
    AAGATGAGAG AATTAACAAT AAACATCAAA ATGAAGGAAG AACTCATTAA AGAGCTGATT
    AAAACAGGTA ACGATGCCCA ATCAGTGAAC AGGCAGTATT CCCTGAAGAT AGCCCATTTG
    GAAGGAGAAG CAGAGCAGGC CAAGTCAGAG CTGTCTGAAG CACAAAGGCA ACTGCAGGAG
    TTGGAGAACA AGGAATTAAG GGACGTGGCG CACAAAACGA AACTGCAGAG AGATTTCCGT
    AAGAAAATGG AAGAAGCAAA ATTAAAAATG CAGGCAGTGC AAACCAAACA ACAGGACACC
    AGGAAGCTCG CCTCCCTCTC CGTCAGGAAC GAAAAGCGCG TGAGAGAACT GGAAGTGAAT
    GTGGAGCAGA TGCGCCAACA GCAAGCCCAA CTGCAGCAAA AGCTCAAAGC CGAAACTGAG
    ATGAAGCGCA AATTAGAGGG CACCATTCAA AAGGAGCAAG AACGGATCAA AGAGCTGCAG
    CTCAAGACCG AGCAGCAGAA CAAGATATTG AGGATGAAAA ATGAAGAGAT TGCTGCAATT
    AGAAGACGAA AGAGCGTTCC CAGTGTCTCT GATAACCTCC AACTTAAACT GGAAGAACAG
    ACCAAGTGGC TTGATGAAGA GGTTGAAAAG GTACTGAAAG AAAGGCAAGC ACTCGCCGAG
    CTGGAAGAGG ATTTAAGGAA AAGGGAAGAA AGCGTCACAA AGATGGAGGC GCTCTTGCAA
    GAAAAAAGCC ACCTGGAGAT GAAGAAGCTT CGATCGAGCC AGGCTATAAC CAGTGACGTG
    CTAAAGCTGT CCAATCGCTT GAATGTTGTG GAGAATGAGC TCATGGATAA AAGCCAATTC
    CTGCAGAACG ACGCTAGTGA TGAGCACAGG AAGGCATCTG AGGAGGTCAA AGAGCTGCAG
    AAGGAACGGG ACGGTCTACT AAAGAGAAGA GATAGCCTGG ACGAGAAGCT GAGAAATGGC
    AGCGTTCTCT CCCCAGAGGA AGAGCACACA CTGTTCCAGC TGGAGGAGGG GATAGAAGCC
    TTGGAAGCTG CGATTGAATA TAAGAACGAA TACATTCAGA ACAGACAGAA GGTTCTGAAA
    GACTCCTCTC AGATTCTCAC CCAAAGTGAA GCCAACATCA TTGAAAAGTT TAGCTCTCTC
    TCGCCTTCAG AAACCCAATC CATCCTTGTC CGATACTTCA ACAAGGTCGT GAGTCTTCGT
    GAAGCAGAGC GGAAACTGCA GCTGCATTCG GCGGAACTGG CCATGAAAGT GACAGAGAAA
    GAGAACATGC TACGAGAGCT GGAATCCTCC CTGGAACATC TCATGCTTCA GAATGATCGA
    AGGCTGACCC AACAGCAGAA GGAGCACGAG CAAAAGCTAC AGCTCCTCTT ACAGCACTTT
    AAGGATAACG ACAATGAAGC AGTTCCAGAA AGCATCAAGG CTTACGAGGC AAAGGTGGCC
    GAGCTGGAGA AGGAGCTGTT CTTTTATAAG AAAACCAGCC GGGAGCTGAA AAAGAGGCTA
    AAAGAGCTGC TTGGGGAACC CAAAGTGGCG GTTTTGAAAA GCAATAACAC TGCTGGAGTC
    TTGGATGAAC CGGGTTCCCC GGCAGCCAAA GAATTGAAGT GGACCCCCCA GCTGGAAAGC
    AACAAAGCTG CCGGAAAAGC AAGAGAGTCC CAACATCCCG ACCTTCAGCG CAATCCTTAC
    AGAGCCGCAG GGGATATTCC CGAAGGTGAC CCTACCTCCA GCTTCCCCAG GCTTCCCTCT
    AGAAGCCATA TCTCCAGAAG GGCCAGCTCA GAAGACGAGG AGCCGGGACA TATTCCCACC
    CAGTCCATAT CAAAGATGGA ACCGAGCAAA AGTCTTTGTG TCACTCCGGT GAAAATATCC
    CGAAGGGAAC TGCGGCAGAT CTCTGCGTCC CACCTATCGA CCCGCCGCTC CAGCCTCACC
    AGCACCCCCG CCGACTCCAT TGAAGTAGCA AGGACTGGAA AAGACTTTAA AGCTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945818.1
    CDS112..3711
    Translation

    Target ORF information:

    RefSeq Version XM_018090329.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 27 (kif27), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018090329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCTTAT TAGCAGCAGG CAGCGCAGCG CGCCATACAG GCAGCACGCA AATGAACGAG 
    AGATCCAGCC GCTCCCACGC CATCTTCACC ATCACTATAT GCCAACAAAG TTCTCAACAC
    GGAGACGACG CTGGCACAAA CTCCATCCAG ACAATCACTT CCAAGTTTCA CTTTGTTGAT
    CTGGCAGGCT CAGAAAGAGT GACCAAAACT GGGAACACTG GGGAAAGGTT TAAGGAATCG
    ATCCAGATAA ATAGTGGTTT ACTTGCCCTT GGAAACGTGA TAAGTGCACT TGGTGACCCG
    AAAAGAAAAA GTGCACATAT TCCTTATAGG GATGCCAAAA TCACTCGTAT ACTAAAGGAC
    TCCTTAGGTG GAAATGCCAA AACAGTAATG ATTACTTGTA TCAGCCCTTC GTCTTCTGAC
    TTAGATGAAT CACTAAACTC TCTGAAATAC GCCAACCGAG CCAGAAACAT TAAAAACAAA
    CCAGTTGTAA ATTACAATCC TGACTGGGAT AGGATGGATG AAATGGAGCT GGAGATCAAG
    GTTCTAAGAG AAGCTCTGCA AAACCAGCAG AGCAGTAGGG TCAGTCGTAC CAGCCAGGTG
    TCCCAAGACT GGAGCCAAGG AAAAGACAAG ATTCGTTCAC TAGAAGAAAA AGTCGCTCAA
    CTCCAGCTGG AATGTTTCAA CCAACGGCAT TGCACCGAGG AAGCATTCTC ATTGTTCCTT
    GATCTAAAGG ATATTTCTGG CTTACCCCAA TCTGAGAAGA ACAGAGTGCA GGACTGGCTT
    CACTTGGCAG AGGGCCTGAG GAGTCCGGTG GCAGCAGATG CCGAGGGCAG TAGCAGAGAA
    GAGCCACATC ATGTCACCAT CTTGCAGCTG AAGAGGGAAC TGAAGAAATG TCAGGATGCC
    CTAGCAACAG ATGAAGAGGT TTTCGCTAGG AAGAAGCTGG AACTGAAAGG GTTACAAGAG
    CAGATTCAGT CGCTACAGCA AGAAAACGAA GAATATCAGA AGTGCCTGCA AGAGGCAGAA
    TATACTAAGA CTTTGCAGAC TGAAAAACTG GTGGAACAGC AGCTCCTTAT TGATGAATTG
    AAGTCAAAGA AGCCGGAAAT CTACAGGCAG TTTTCGCAGG AAAATGAAGA TGAAGAGGAA
    CTTCCAAGGG AAATATCCCA AAGGCCACAC ACTGTGCCCC CCAGCACCCA CACCCAGAAA
    CTCTCGTCCA GTTCATTGCA GGCTGCAGGG CAGGCAGAGC CAAGGAAGGT ACGAACAAGC
    CCCCCTTCCT ATTCCCTAGA AAGAATGGTA GCGGGATTCC GGACTCGCAG CCAGATGCTC
    CTGGAGCTCA TAGAGGAGCA GGATGAAGTT CTGCATGAGA AGTTCTCAGA CCAAAGTGAA
    GAAGAACCTG ATGAGCCAGA GAAAGAAAAC AGAAAGAAAT CACCATTTAG GCGGTCTATC
    AATCGGACAT GGACACGACG ACAAGGCTCT GCTGGTGCGG AAACACTTAA CTCCAACTCA
    GACAGAGAGA ATGACGCAAA CTCTAATGCT TGTCCAGCTC TGGAATCCCT GCGAGAAAGT
    CAGGTGCTGA ATGTGGAGAA ACTGAAAACC TCAGACGTTC GCCTCGCTGA TGCCAAGCAG
    AAGATGAGAG AATTAACAAT AAACATCAAA ATGAAGGAAG AACTCATTAA AGAGCTGATT
    AAAACAGGTA ACGATGCCCA ATCAGTGAAC AGGCAGTATT CCCTGAAGAT AGCCCATTTG
    GAAGGAGAAG CAGAGCAGGC CAAGTCAGAG CTGTCTGAAG CACAAAGGCA ACTGCAGGAG
    TTGGAGAACA AGGAATTAAG GGACGTGGCG CACAAAACGA AACTGCAGAG AGATTTCCGT
    AAGAAAATGG AAGAAGCAAA ATTAAAAATG CAGGCAGTGC AAACCAAACA ACAGGACACC
    AGGAAGCTCG CCTCCCTCTC CGTCAGGAAC GAAAAGCGCG TGAGAGAACT GGAAGTGAAT
    GTGGAGCAGA TGCGCCAACA GCAAGCCCAA CTGCAGCAAA AGCTCAAAGC CGAAACTGAG
    ATGAAGCGCA AATTAGAGGG CACCATTCAA AAGGAGCAAG AACGGATCAA AGAGCTGCAG
    CTCAAGACCG AGCAGCAGAA CAAGATATTG AGGATGAAAA ATGAAGAGAT TGCTGCAATT
    AGAAGACGAA AGAGCGTTCC CAGTGTCTCT GATAACCTCC AACTTAAACT GGAAGAACAG
    ACCAAGTGGC TTGATGAAGA GGTTGAAAAG GTACTGAAAG AAAGGCAAGC ACTCGCCGAG
    CTGGAAGAGG ATTTAAGGAA AAGGGAAGAA AGCGTCACAA AGATGGAGGC GCTCTTGCAA
    GAAAAAAGCC ACCTGGAGAT GAAGAAGCTT CGATCGAGCC AGGCTATAAC CAGTGACGTG
    CTAAAGCTGT CCAATCGCTT GAATGTTGTG GAGAATGAGC TCATGGATAA AAGCCAATTC
    CTGCAGAACG ACGCTAGTGA TGAGCACAGG AAGGCATCTG AGGAGGTCAA AGAGCTGCAG
    AAGGAACGGG ACGGTCTACT AAAGAGAAGA GATAGCCTGG ACGAGAAGCT GAGAAATGGC
    AGCGTTCTCT CCCCAGAGGA AGAGCACACA CTGTTCCAGC TGGAGGAGGG GATAGAAGCC
    TTGGAAGCTG CGATTGAATA TAAGAACGAA TACATTCAGA ACAGACAGAA GGTTCTGAAA
    GACTCCTCTC AGATTCTCAC CCAAAGTGAA GCCAACATCA TTGAAAAGTT TAGCTCTCTC
    TCGCCTTCAG AAACCCAATC CATCCTTGTC CGATACTTCA ACAAGGTCGT GAGTCTTCGT
    GAAGCAGAGC GGAAACTGCA GCTGCATTCG GCGGAACTGG CCATGAAAGT GACAGAGAAA
    GAGAACATGC TACGAGAGCT GGAATCCTCC CTGGAACATC TCATGCTTCA GAATGATCGA
    AGGCTGACCC AACAGCAGAA GGAGCACGAG CAAAAGCTAC AGCTCCTCTT ACAGCACTTT
    AAGGATAACG ACAATGAAGC AGTTCCAGAA AGCATCAAGG CTTACGAGGC AAAGGTGGCC
    GAGCTGGAGA AGGAGCTGTT CTTTTATAAG AAAACCAGCC GGGAGCTGAA AAAGAGGCTA
    AAAGAGCTGC TTGGGGAACC CAAAGTGGCG GTTTTGAAAA GCAATAACAC TGCTGGAGTC
    TTGGATGAAC CGGGTTCCCC GGCAGCCAAA GAATTGAAGT GGACCCCCCA GCTGGAAAGC
    AACAAAGCTG CCGGAAAAGC AAGAGAGTCC CAACATCCCG ACCTTCAGCG CAATCCTTAC
    AGAGCCGCAG GGGATATTCC CGAAGGTGAC CCTACCTCCA GCTTCCCCAG GCTTCCCTCT
    AGAAGCCATA TCTCCAGAAG GGCCAGCTCA GAAGACGAGG AGCCGGGACA TATTCCCACC
    CAGTCCATAT CAAAGATGGA ACCGAGCAAA AGTCTTTGTG TCACTCCGGT GAAAATATCC
    CGAAGGGAAC TGCGGCAGAT CTCTGCGTCC CACCTATCGA CCCGCCGCTC CAGCCTCACC
    AGCACCCCCG CCGACTCCAT TGAAGTAGCA AGGACTGGAA AAGACTTTAA AGCTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49291
    Clone ID Related Accession (Same CDS sequence) XM_031894957.1
    Accession Version XM_031894957.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAACAC GACAAGAAAC TCCAGACAAA ATCACAATGG AGGAGACTCC AGTCAAAGTG 
    GCTTTGAGGG TCCGACCTCT GCTGTCCAGG GAGATCCTTC ACAATCACCA AGTGTGTGTC
    CGGCTCGTCC CAAACACCCA GCAGGTCATC GTCGGGAAGG ATCGGGTTTT CACCTTTGAC
    TTTGTGTTTG GCAAGAGCTC CTCCCAGGAC GACGTCTACA GGTCGAGCAT CAAACCACTT
    CTGGGGTCAT TAATGGAAGG GTACAACGCC ACTGTGTTTG CCTACGGGCA GACCGGCTCC
    GGCAAGACTT ACACTATTGG AGGAGGAAAT GTCGCTTCCG TTGCAGATGA GGAAAAGGGC
    ATCATTCCCC GTGCCATCCA GGAACTGTTC CAGTGCATCG CAGAACGCCA TAATGTTGAA
    TTCAGCGTTA GAGTGTCGTA CATTGAAGTG TACAAGGAGG AGCTGCTGGA TCTGCTGGAG
    CTGGAAACCA ACATGAAGGA TCTACACATT AGGGAGGATG AGAAAGGGAA CACCGTCATC
    GTTGGTGCCA AAGAATGTCA AGTGGAAAAT GCTGATGAAG TCATGAGCTT ATTAGCAGCA
    GGCAGCGCAG CGCGCCATAC AGGCAGCACG CAAATGAACG AGAGATCCAG CCGCTCCCAC
    GCCATCTTCA CCATCACTAT ATGCCAACAA AGTTCTCAAC ACGGAGACGA CGCTGGCACA
    AACTCCATCC AGACAATCAC TTCCAAGTTT CACTTTGTTG ATCTGGCAGG CTCAGAAAGA
    GTGACCAAAA CTGGGAACAC TGGGGAAAGG TTTAAGGAAT CGATCCAGAT AAATAGTGGT
    TTACTTGCCC TTGGAAACGT GATAAGTGCA CTTGGTGACC CGAAAAGAAA AAGTGCACAT
    ATTCCTTATA GGGATGCCAA AATCACTCGT ATACTAAAGG ACTCCTTAGG TGGAAATGCC
    AAAACAGTAA TGATTACTTG TATCAGCCCT TCGTCTTCTG ACTTAGATGA ATCACTAAAC
    TCTCTGAAAT ACGCCAACCG AGCCAGAAAC ATTAAAAACA AACCAGTTGT AAATTACAAT
    CCTGACTGGG ATAGGATGGA TGAAATGGAG CTGGAGATCA AGGTTCTAAG AGAAGCTCTG
    CAAAACCAGC AGAGCAGTAG GGTCAGTCGT ACCAGCCAGG TGTCCCAAGA CTGGAGCCAA
    GGAAAAGACA AGATTCGTTC ACTAGAAGAA AAAGTCGCTC AACTCCAGCT GGAATGTTTC
    AACCAACGGC ATTGCACCGA GGAAGCATTC TCATTGTTCC TTGATCTAAA GGATATTTCT
    GGCTTACCCC AATCTGAGAA GAACAGAGTG CAGGACTGGC TTCACTTGGC AGAGGGCCTG
    AGGAGTCCGG TGGCAGCAGA TGCCGAGGGC AGTAGCAGAG AAGAGCCACA TCATGTCACC
    ATCTTGCAGC TGAAGAGGGA ACTGAAGAAA TGTCAGGATG CCCTAGCAAC AGATGAAGAG
    GTTTTCGCTA GGAAGAAGCT GGAACTGAAA GGGTTACAAG AGCAAATTCA GTCGCTACAG
    CAAGAAACCG AAGAATATCA GAAGTGCCTG CAAGAGGCAG AATATACTAA GACTTTGCAG
    ACTGAAAAAC TGGTGGAACA GCAGCTCCTT ATTGATGAAT TGAAGTCAAA GAAGCCGGAA
    ATCTACAGGC AGTTTTCGCA GGAAAATGAA GATGAAGAGG AACTTCCAAG GGAAATATCC
    CAAAGGCCAC ACACTGTGCC CCCCAGCACC CACACCCAGA AACTCTCGTC CAGTTCATTG
    CAGGCTGCAG GGCAGGCAGA GCCAAGGAAG GTACGAACAA GCCCCCCTTC CTATTCCCTA
    GAAAGAATGG TAGCGGGATT CCGGACTCGC AGCCAGATGC TCCTGGAGCT CATAGAGGAG
    CAGGATGAAG TTCTGCATGA GAAGTTCTCA GACCAAAGTG AAGAAGAACC TGATGAGCCA
    GAGAAAGAAA ACAGAAAGAA ATCACCATTT AGGCGGTCTA TCAATCGGAC ATGGACACGA
    CGACAAGGCT CTGCCGGTGC GGAAACACTT AACTCCAACT CAGACAGAGA GAATGACGCA
    AACTCTAATG CTTGTCCAGC TCTGGAATCC CTGCGAGAAA GTCAGGTGCT GAATGTGGAG
    AAACTGAAAA CCTCAGACGT TCGCCTCGCT GATGCCAAGC AGAAGATGAG AGAATTAACA
    ATAAACATCA AAATGAAGGA AGAACTCATT AAAGAGCTGA TTAAAACAGG TAACGATGCC
    CAATCAGTGA ACAGGCAGTA TTCCCTGAAG ATAGCCCATC TGGAAGGAGA AGCAGAGCAG
    GCCAAGTCAG AGCTGTCTGA AGCACAAAGG CAACTGCAGG AGTTGGAGAA CAAGGAATTA
    AGGGACGTGG CGCACAAAAC GAAACTGCAG AGAGATTTCC GTAAGAAAAT GGAAGAAGCA
    AAATTAAAAA TGCAGGCAGT GCAAACCAAA CAACAGGACA CCAGGAAGCT CGCCTCCCTC
    TCCGTCAGGA ACGAAAAGCG CGTGAGAGAA CTGGAAGTGA ATGTGGAGCA GATGCGCCAA
    CAGCAAGCCC AACTGCAGCG AAAGCTCAAA GCCGAAACTG AGATGAAGCG CAAATTAGAG
    GGCACCATTC AAAAGGAGCA AGAACGGATC AAAGAGCTGC AGCTCAAGAC CGAGCAGCAG
    AACAAGATAT TGAGGATGAA AAATGAAGAG ATTGCTGCAA TTAGAAGACG AAAGAGCATT
    CCCAGTGTCT CTGATAACCT CCAACTTAAA CTGGAAGAAC AGACCAAGTG GCTTGATGAA
    GAGGTTGAAA AGGTACTGAA AGAAAGGCAA GCACTCGCCG AGCTGGAAGA GGATTTAAGG
    AAAAGGGAAG AAAGCGTCAC AAAGATGGAG GCGCTCTTGC AAGAAAAAAG CCACCTGGAG
    ATGAAGAAGC TTCGATCGAG CCAGGCTATA ACCAGTGACG TGCTAAAGCT GTCCAATCGC
    TTGAATGTTG TGGAGAATGA GCTCATGGAT AAAAGCCAAT TCCTGCAGAA CGACGCTAGT
    GATGAGCACA GGAAGGCATC TGAGGAGGTC AAAGAGCTGC AGAAGGAACG GGACGGTCTA
    CTAAAGAGAA GAGATAGCCT GGACGAGAAG CTGAGAAATG GCAGCGTTCT CTCCCCAGAG
    GAAGAGCACA CACTGTTCCA GCTGGAGGAG GGGATAGAAG CCTTGGAAGC TGCGATTGAA
    TATAAGAACG AATACATTCA GAACAGACAG AAGGTTCTGA AAGACTCCTC TCAGATTCTC
    ACCCAAAGTG AAGCCAACAT CATTGAAAAG TTTAGCTCTC TCTCGCCTTC AGAAACCCAA
    TCCATCCTTG TCCGATACTT CAACAAGGTC GTGAGTCTTC GTGAAGCAGA GCGGAAACTG
    CAGCTGCATT CGGCGGAACT GGCCATGAAA GTGACAGAGA AAGAGAACAT GTTACGAGAG
    CTGGAATCCT CCCTGGAACA TCTCATGCTT CAGAATGATC GAAGGCTGAC CCAACAGCAG
    AAGGAGCACG AGCAAAAGCT ACAGCTCCTC TTACAGCACT TTAAGGATAA CGACAATGAA
    GCAGTTCCAG AAAGCATCAA GGCTTACGAG GCAAAGGTGG CCGAGCTGGA GAAGGAGCTG
    TTCTTTTATA AGAAAACCAG CCGGGAGCTG AAAAAGAGGC TAAAAGAGCT GCTTGGGGAA
    CCCAAAGTGG CGGTTTTGAA AAGCAATAAC ACTGCTGGAG TCTTGGATGA ACCGGGTTCC
    CCGGCAGCCA AAGAATTGAA GTGGACCCCC CAGCTGGAAA GCAACAAAGC TGCCGGAAAA
    GCAAGAGAGT CCCAACATCC CGACCTTCAG CGCAATCCTT ACAGAGCCGC AGGGGATATT
    CCCGAAGGTG ACCCTACCTC CAGCTTCCCC AGGCTTCCCT CTAGAAGCCA TATCTCCAGA
    AGGGCCAGCT CAGAAGACGA GGAGCCGGGA CATATTCCCA CCCAGTCCAT ATCAAAGATG
    GAACCGAGCA AAAGTCTTTG TGTCACTCCG GTGAAAATAT CCCGAAGGGA ACTGCGGCAG
    ATCTCTGCGT CCCACCTATC GACCCGCCGC TCCAGCCTCA CCAGCACCCC CGCCGACTCC
    ATTGAAGTAG CAAGGACTGG AAAAGACTTT AAAGCTCAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750817.1
    CDS116..4297
    Translation

    Target ORF information:

    RefSeq Version XM_031894957.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 27 (kif27), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894957.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAACAC GACAAGAAAC TCCAGACAAA ATCACAATGG AGGAGACTCC AGTCAAAGTG 
    GCTTTGAGGG TCCGACCTCT GCTGTCCAGG GAGATCCTTC ACAATCACCA AGTGTGTGTC
    CGGCTCGTCC CAAACACCCA GCAGGTCATC GTCGGGAAGG ATCGGGTTTT CACCTTTGAC
    TTTGTGTTTG GCAAGAGCTC CTCCCAGGAC GACGTCTACA GGTCGAGCAT CAAACCACTT
    CTGGGGTCAT TAATGGAAGG GTACAACGCC ACTGTGTTTG CCTACGGGCA GACCGGCTCC
    GGCAAGACTT ACACTATTGG AGGAGGAAAT GTCGCTTCCG TTGCAGATGA GGAAAAGGGC
    ATCATTCCCC GTGCCATCCA GGAACTGTTC CAGTGCATCG CAGAACGCCA TAATGTTGAA
    TTCAGCGTTA GAGTGTCGTA CATTGAAGTG TACAAGGAGG AGCTGCTGGA TCTGCTGGAG
    CTGGAAACCA ACATGAAGGA TCTACACATT AGGGAGGATG AGAAAGGGAA CACCGTCATC
    GTTGGTGCCA AAGAATGTCA AGTGGAAAAT GCTGATGAAG TCATGAGCTT ATTAGCAGCA
    GGCAGCGCAG CGCGCCATAC AGGCAGCACG CAAATGAACG AGAGATCCAG CCGCTCCCAC
    GCCATCTTCA CCATCACTAT ATGCCAACAA AGTTCTCAAC ACGGAGACGA CGCTGGCACA
    AACTCCATCC AGACAATCAC TTCCAAGTTT CACTTTGTTG ATCTGGCAGG CTCAGAAAGA
    GTGACCAAAA CTGGGAACAC TGGGGAAAGG TTTAAGGAAT CGATCCAGAT AAATAGTGGT
    TTACTTGCCC TTGGAAACGT GATAAGTGCA CTTGGTGACC CGAAAAGAAA AAGTGCACAT
    ATTCCTTATA GGGATGCCAA AATCACTCGT ATACTAAAGG ACTCCTTAGG TGGAAATGCC
    AAAACAGTAA TGATTACTTG TATCAGCCCT TCGTCTTCTG ACTTAGATGA ATCACTAAAC
    TCTCTGAAAT ACGCCAACCG AGCCAGAAAC ATTAAAAACA AACCAGTTGT AAATTACAAT
    CCTGACTGGG ATAGGATGGA TGAAATGGAG CTGGAGATCA AGGTTCTAAG AGAAGCTCTG
    CAAAACCAGC AGAGCAGTAG GGTCAGTCGT ACCAGCCAGG TGTCCCAAGA CTGGAGCCAA
    GGAAAAGACA AGATTCGTTC ACTAGAAGAA AAAGTCGCTC AACTCCAGCT GGAATGTTTC
    AACCAACGGC ATTGCACCGA GGAAGCATTC TCATTGTTCC TTGATCTAAA GGATATTTCT
    GGCTTACCCC AATCTGAGAA GAACAGAGTG CAGGACTGGC TTCACTTGGC AGAGGGCCTG
    AGGAGTCCGG TGGCAGCAGA TGCCGAGGGC AGTAGCAGAG AAGAGCCACA TCATGTCACC
    ATCTTGCAGC TGAAGAGGGA ACTGAAGAAA TGTCAGGATG CCCTAGCAAC AGATGAAGAG
    GTTTTCGCTA GGAAGAAGCT GGAACTGAAA GGGTTACAAG AGCAAATTCA GTCGCTACAG
    CAAGAAACCG AAGAATATCA GAAGTGCCTG CAAGAGGCAG AATATACTAA GACTTTGCAG
    ACTGAAAAAC TGGTGGAACA GCAGCTCCTT ATTGATGAAT TGAAGTCAAA GAAGCCGGAA
    ATCTACAGGC AGTTTTCGCA GGAAAATGAA GATGAAGAGG AACTTCCAAG GGAAATATCC
    CAAAGGCCAC ACACTGTGCC CCCCAGCACC CACACCCAGA AACTCTCGTC CAGTTCATTG
    CAGGCTGCAG GGCAGGCAGA GCCAAGGAAG GTACGAACAA GCCCCCCTTC CTATTCCCTA
    GAAAGAATGG TAGCGGGATT CCGGACTCGC AGCCAGATGC TCCTGGAGCT CATAGAGGAG
    CAGGATGAAG TTCTGCATGA GAAGTTCTCA GACCAAAGTG AAGAAGAACC TGATGAGCCA
    GAGAAAGAAA ACAGAAAGAA ATCACCATTT AGGCGGTCTA TCAATCGGAC ATGGACACGA
    CGACAAGGCT CTGCCGGTGC GGAAACACTT AACTCCAACT CAGACAGAGA GAATGACGCA
    AACTCTAATG CTTGTCCAGC TCTGGAATCC CTGCGAGAAA GTCAGGTGCT GAATGTGGAG
    AAACTGAAAA CCTCAGACGT TCGCCTCGCT GATGCCAAGC AGAAGATGAG AGAATTAACA
    ATAAACATCA AAATGAAGGA AGAACTCATT AAAGAGCTGA TTAAAACAGG TAACGATGCC
    CAATCAGTGA ACAGGCAGTA TTCCCTGAAG ATAGCCCATC TGGAAGGAGA AGCAGAGCAG
    GCCAAGTCAG AGCTGTCTGA AGCACAAAGG CAACTGCAGG AGTTGGAGAA CAAGGAATTA
    AGGGACGTGG CGCACAAAAC GAAACTGCAG AGAGATTTCC GTAAGAAAAT GGAAGAAGCA
    AAATTAAAAA TGCAGGCAGT GCAAACCAAA CAACAGGACA CCAGGAAGCT CGCCTCCCTC
    TCCGTCAGGA ACGAAAAGCG CGTGAGAGAA CTGGAAGTGA ATGTGGAGCA GATGCGCCAA
    CAGCAAGCCC AACTGCAGCG AAAGCTCAAA GCCGAAACTG AGATGAAGCG CAAATTAGAG
    GGCACCATTC AAAAGGAGCA AGAACGGATC AAAGAGCTGC AGCTCAAGAC CGAGCAGCAG
    AACAAGATAT TGAGGATGAA AAATGAAGAG ATTGCTGCAA TTAGAAGACG AAAGAGCATT
    CCCAGTGTCT CTGATAACCT CCAACTTAAA CTGGAAGAAC AGACCAAGTG GCTTGATGAA
    GAGGTTGAAA AGGTACTGAA AGAAAGGCAA GCACTCGCCG AGCTGGAAGA GGATTTAAGG
    AAAAGGGAAG AAAGCGTCAC AAAGATGGAG GCGCTCTTGC AAGAAAAAAG CCACCTGGAG
    ATGAAGAAGC TTCGATCGAG CCAGGCTATA ACCAGTGACG TGCTAAAGCT GTCCAATCGC
    TTGAATGTTG TGGAGAATGA GCTCATGGAT AAAAGCCAAT TCCTGCAGAA CGACGCTAGT
    GATGAGCACA GGAAGGCATC TGAGGAGGTC AAAGAGCTGC AGAAGGAACG GGACGGTCTA
    CTAAAGAGAA GAGATAGCCT GGACGAGAAG CTGAGAAATG GCAGCGTTCT CTCCCCAGAG
    GAAGAGCACA CACTGTTCCA GCTGGAGGAG GGGATAGAAG CCTTGGAAGC TGCGATTGAA
    TATAAGAACG AATACATTCA GAACAGACAG AAGGTTCTGA AAGACTCCTC TCAGATTCTC
    ACCCAAAGTG AAGCCAACAT CATTGAAAAG TTTAGCTCTC TCTCGCCTTC AGAAACCCAA
    TCCATCCTTG TCCGATACTT CAACAAGGTC GTGAGTCTTC GTGAAGCAGA GCGGAAACTG
    CAGCTGCATT CGGCGGAACT GGCCATGAAA GTGACAGAGA AAGAGAACAT GTTACGAGAG
    CTGGAATCCT CCCTGGAACA TCTCATGCTT CAGAATGATC GAAGGCTGAC CCAACAGCAG
    AAGGAGCACG AGCAAAAGCT ACAGCTCCTC TTACAGCACT TTAAGGATAA CGACAATGAA
    GCAGTTCCAG AAAGCATCAA GGCTTACGAG GCAAAGGTGG CCGAGCTGGA GAAGGAGCTG
    TTCTTTTATA AGAAAACCAG CCGGGAGCTG AAAAAGAGGC TAAAAGAGCT GCTTGGGGAA
    CCCAAAGTGG CGGTTTTGAA AAGCAATAAC ACTGCTGGAG TCTTGGATGA ACCGGGTTCC
    CCGGCAGCCA AAGAATTGAA GTGGACCCCC CAGCTGGAAA GCAACAAAGC TGCCGGAAAA
    GCAAGAGAGT CCCAACATCC CGACCTTCAG CGCAATCCTT ACAGAGCCGC AGGGGATATT
    CCCGAAGGTG ACCCTACCTC CAGCTTCCCC AGGCTTCCCT CTAGAAGCCA TATCTCCAGA
    AGGGCCAGCT CAGAAGACGA GGAGCCGGGA CATATTCCCA CCCAGTCCAT ATCAAAGATG
    GAACCGAGCA AAAGTCTTTG TGTCACTCCG GTGAAAATAT CCCGAAGGGA ACTGCGGCAG
    ATCTCTGCGT CCCACCTATC GACCCGCCGC TCCAGCCTCA CCAGCACCCC CGCCGACTCC
    ATTGAAGTAG CAAGGACTGG AAAAGACTTT AAAGCTCAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36894
    Clone ID Related Accession (Same CDS sequence) XM_012955029.2
    Accession Version XM_012955029.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF27 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683785.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAACAC GACAAGAAAC TCCAGACAAG ATCACAATGG AGGAGACTCC AGTCAAAGTG 
    GCTTTGAGGG TCCGACCTCT GCTGTCCAGG GAGATCCTTC ACAATCACCA AGTGTGTGTC
    CGGCTCGTCC CAAACACCCA GCAGGTCATC GTCGGGAAGG ATCGGGTTTT CACCTTTGAC
    TTTGTGTTTG GCAAGAGCTC CTCCCAGGAC GACGTCTACA GGTCGAGCAT CAAACCGCTT
    CTGGGGTCAT TAATGGAAGG GTACAACGCC ACTGTGTTTG CCTACGGGCA GACCGGCTCC
    GGCAAGACTT ACACTATTGG AGGAGGAAAT GTCGCTTCCG TTGCAGATGA GGAAAAGGGC
    ATCATTCCCC GTGCCATCCA GGAACTGTTC CAGTGCATCG CAGAACGCCA TAATGTTGAA
    TTCAGCGTTA GAGTGTCGTA CATTGAAGTG TACAAGGAGG AGCTGCTGGA TTTGCTGGAG
    CTGGAAACCA ACATGAAGGA TCTACACATT AGGGAGGATG AGAAAGGGAA CACCGTCATC
    GTTGGTGCCA AAGAATGTCA AGTGGAAAAT GCTGATGAAG TCATGAGCTT ATTAGCAGCA
    GGCAGCGCAG CGCGCCATAC AGGCAGCACG CAAATGAACG AGAGATCCAG CCGCTCCCAC
    GCCATCTTCA CCATCACTAT ATGCCAACAA AGTTCTCAAC ACGGAGACGA CGCTGGCACA
    AACTCCATCC AGACAATCAC TTCCAAGTTT CACTTTGTTG ATCTGGCAGG CTCAGAAAGA
    GTGACCAAAA CTGGGAACAC TGGGGAAAGG TTTAAGGAAT CGATCCAGAT AAATAGTGGT
    TTACTTGCCC TTGGAAACGT GATAAGTGCA CTTGGTGACC CGAAAAGAAA AAGTGCACAT
    ATTCCTTATA GGGATGCCAA AATCACTCGT ATACTAAAGG ACTCCTTAGG TGGAAATGCC
    AAAACAGTAA TGATTACTTG TATCAGCCCT TCGTCTTCTG ACTTAGATGA ATCACTAAAC
    TCTCTGAAAT ACGCCAACCG AGCCAGAAAC ATTAAAAACA AACCAGTTGT AAATTACAAT
    CCTGACTGGG ATAGGATGGA TGAAATGGAG CTGGAGATCA AGGTTCTAAG AGAAGCTCTG
    CAAAACCAGC AGAGCAGTAG GGTCAGTCGT ACCAGCCAGG TGTCCCAAGA CTGGAGCCAA
    GGAAAAGACA AGATTCGTTC ACTAGAAGAA AAAGTCGCTC AACTCCAGCT GGAATGTTTC
    AACCAACGGC ATTGCACCGA GGAAGCATTC TCATTGTTCC TTGATCTAAA GGATATTTCT
    GGCTTACCCC AATCTGAGAA GAACAGAGTG CAGGACTGGC TTCACTTGGC AGAGGGCCTG
    AGGAGTCCGG TGGCAGCAGA TGCCGAGGGC AGTAGCAGAG AAGAGCCACA TCATGTCACC
    ATCTTGCAGC TGAAGAGGGA ACTGAAGAAA TGTCAGGATG CCCTAGCAAC AGATGAAGAG
    GTTTTCGCTA GGAAGAAGCT GGAACTGAAA GGGTTACAAG AGCAGATTCA GTCGCTACAG
    CAAGAAAACG AAGAATATCA GAAGTGCCTG CAAGAGGCAG AATATACTAA GACTTTGCAG
    ACTGAAAAAC TGGTGGAACA GCAGCTCCTT ATTGATGAAT TGAAGTCAAA GAAGCCGGAA
    ATCTACAGGC AGTTTTCGCA GGAAAATGAA GATGAAGAGG AACTTCCAAG GGAAATATCC
    CAAAGGCCAC ACACTGTGCC CCCCAGCACC CACACCCAGA AACTCTCGTC CAGTTCATTG
    CAGGCTGCAG GGCAGGCAGA GCCAAGGAAG GTACGAACAA GCCCCCCTTC CTATTCCCTA
    GAAAGAATGG TAGCGGGATT CCGGACTCGC AGCCAGATGC TCCTGGAGCT CATAGAGGAG
    CAGGATGAAG TTCTGCATGA GAAGTTCTCA GACCAAAGTG AAGAAGAACC TGATGAGCCA
    GAGAAAGAAA ACAGAAAGAA ATCACCATTT AGGCGGTCTA TCAATCGGAC ATGGACACGA
    CGACAAGGCT CTGCTGGTGC GGAAACACTT AACTCCAACT CAGACAGAGA GAATGACGCA
    AACTCTAATG CTTGTCCAGC TCTGGAATCC CTGCGAGAAA GTCAGGTGCT GAATGTGGAG
    AAACTGAAAA CCTCAGACGT TCGCCTCGCT GATGCCAAGC AGAAGATGAG AGAATTAACA
    ATAAACATCA AAATGAAGGA AGAACTCATT AAAGAGCTGA TTAAAACAGG TAACGATGCC
    CAATCAGTGA ACAGGCAGTA TTCCCTGAAG ATAGCCCATT TGGAAGGAGA AGCAGAGCAG
    GCCAAGTCAG AGCTGTCTGA AGCACAAAGG CAACTGCAGG AGTTGGAGAA CAAGGAATTA
    AGGGACGTGG CGCACAAAAC GAAACTGCAG AGAGATTTCC GTAAGAAAAT GGAAGAAGCA
    AAATTAAAAA TGCAGGCAGT GCAAACCAAA CAACAGGACA CCAGGAAGCT CGCCTCCCTC
    TCCGTCAGGA ACGAAAAGCG CGTGAGAGAA CTGGAAGTGA ATGTGGAGCA GATGCGCCAA
    CAGCAAGCCC AACTGCAGCA AAAGCTCAAA GCCGAAACTG AGATGAAGCG CAAATTAGAG
    GGCACCATTC AAAAGGAGCA AGAACGGATC AAAGAGCTGC AGCTCAAGAC CGAGCAGCAG
    AACAAGATAT TGAGGATGAA AAATGAAGAG ATTGCTGCAA TTAGAAGACG AAAGAGCGTT
    CCCAGTGTCT CTGATAACCT CCAACTTAAA CTGGAAGAAC AGACCAAGTG GCTTGATGAA
    GAGGTTGAAA AGGTACTGAA AGAAAGGCAA GCACTCGCCG AGCTGGAAGA GGATTTAAGG
    AAAAGGGAAG AAAGCGTCAC AAAGATGGAG GCGCTCTTGC AAGAAAAAAG CCACCTGGAG
    ATGAAGAAGC TTCGATCGAG CCAGGCTATA ACCAGTGACG TGCTAAAGCT GTCCAATCGC
    TTGAATGTTG TGGAGAATGA GCTCATGGAT AAAAGCCAAT TCCTGCAGAA CGACGCTAGT
    GATGAGCACA GGAAGGCATC TGAGGAGGTC AAAGAGCTGC AGAAGGAACG GGACGGTCTA
    CTAAAGAGAA GAGATAGCCT GGACGAGAAG CTGAGAAATG GCAGCGTTCT CTCCCCAGAG
    GAAGAGCACA CACTGTTCCA GCTGGAGGAG GGGATAGAAG CCTTGGAAGC TGCGATTGAA
    TATAAGAACG AATACATTCA GAACAGACAG AAGGTTCTGA AAGACTCCTC TCAGATTCTC
    ACCCAAAGTG AAGCCAACAT CATTGAAAAG TTTAGCTCTC TCTCGCCTTC AGAAACCCAA
    TCCATCCTTG TCCGATACTT CAACAAGGTC GTGAGTCTTC GTGAAGCAGA GCGGAAACTG
    CAGCTGCATT CGGCGGAACT GGCCATGAAA GTGACAGAGA AAGAGAACAT GCTACGAGAG
    CTGGAATCCT CCCTGGAACA TCTCATGCTT CAGAATGATC GAAGGCTGAC CCAACAGCAG
    AAGGAGCACG AGCAAAAGCT ACAGCTCCTC TTACAGCACT TTAAGGATAA CGACAATGAA
    GCAGTTCCAG AAAGCATCAA GGCTTACGAG GCAAAGGTGG CCGAGCTGGA GAAGGAGCTG
    TTCTTTTATA AGAAAACCAG CCGGGAGCTG AAAAAGAGGC TAAAAGAGCT GCTTGGGGAA
    CCCAAAGTGG CGGTTTTGAA AAGCAATAAC ACTGCTGGAG TCTTGGATGA ACCGGGTTCC
    CCGGCAGCCA AAGAATTGAA GTGGACCCCC CAGCTGGAAA GCAACAAAGC TGCCGGAAAA
    GCAAGAGAGT CCCAACATCC CGACCTTCAG CGCAATCCTT ACAGAGCCGC AGGGGATATT
    CCCGAAGGTG ACCCTACCTC CAGCTTCCCC AGGCTTCCCT CTAGAAGCCA TATCTCCAGA
    AGGGCCAGCT CAGAAGACGA GGAGCCGGGA CATATTCCCA CCCAGTCCAT ATCAAAGATG
    GAACCGAGCA AAAGTCTTTG TGTCACTCCG GTGAAAATAT CCCGAAGGGA ACTGCGGCAG
    ATCTCTGCGT CCCACCTATC GACCCGCCGC TCCAGCCTCA CCAGCACCCC CGCCGACTCC
    ATTGAAGTAG CAAGGACTGG AAAAGACTTT AAAGCTCAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012810483.1
    CDS77..4258
    Translation

    Target ORF information:

    RefSeq Version XM_012955029.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 27 (kif27), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012955029.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAACAC GACAAGAAAC TCCAGACAAG ATCACAATGG AGGAGACTCC AGTCAAAGTG 
    GCTTTGAGGG TCCGACCTCT GCTGTCCAGG GAGATCCTTC ACAATCACCA AGTGTGTGTC
    CGGCTCGTCC CAAACACCCA GCAGGTCATC GTCGGGAAGG ATCGGGTTTT CACCTTTGAC
    TTTGTGTTTG GCAAGAGCTC CTCCCAGGAC GACGTCTACA GGTCGAGCAT CAAACCGCTT
    CTGGGGTCAT TAATGGAAGG GTACAACGCC ACTGTGTTTG CCTACGGGCA GACCGGCTCC
    GGCAAGACTT ACACTATTGG AGGAGGAAAT GTCGCTTCCG TTGCAGATGA GGAAAAGGGC
    ATCATTCCCC GTGCCATCCA GGAACTGTTC CAGTGCATCG CAGAACGCCA TAATGTTGAA
    TTCAGCGTTA GAGTGTCGTA CATTGAAGTG TACAAGGAGG AGCTGCTGGA TTTGCTGGAG
    CTGGAAACCA ACATGAAGGA TCTACACATT AGGGAGGATG AGAAAGGGAA CACCGTCATC
    GTTGGTGCCA AAGAATGTCA AGTGGAAAAT GCTGATGAAG TCATGAGCTT ATTAGCAGCA
    GGCAGCGCAG CGCGCCATAC AGGCAGCACG CAAATGAACG AGAGATCCAG CCGCTCCCAC
    GCCATCTTCA CCATCACTAT ATGCCAACAA AGTTCTCAAC ACGGAGACGA CGCTGGCACA
    AACTCCATCC AGACAATCAC TTCCAAGTTT CACTTTGTTG ATCTGGCAGG CTCAGAAAGA
    GTGACCAAAA CTGGGAACAC TGGGGAAAGG TTTAAGGAAT CGATCCAGAT AAATAGTGGT
    TTACTTGCCC TTGGAAACGT GATAAGTGCA CTTGGTGACC CGAAAAGAAA AAGTGCACAT
    ATTCCTTATA GGGATGCCAA AATCACTCGT ATACTAAAGG ACTCCTTAGG TGGAAATGCC
    AAAACAGTAA TGATTACTTG TATCAGCCCT TCGTCTTCTG ACTTAGATGA ATCACTAAAC
    TCTCTGAAAT ACGCCAACCG AGCCAGAAAC ATTAAAAACA AACCAGTTGT AAATTACAAT
    CCTGACTGGG ATAGGATGGA TGAAATGGAG CTGGAGATCA AGGTTCTAAG AGAAGCTCTG
    CAAAACCAGC AGAGCAGTAG GGTCAGTCGT ACCAGCCAGG TGTCCCAAGA CTGGAGCCAA
    GGAAAAGACA AGATTCGTTC ACTAGAAGAA AAAGTCGCTC AACTCCAGCT GGAATGTTTC
    AACCAACGGC ATTGCACCGA GGAAGCATTC TCATTGTTCC TTGATCTAAA GGATATTTCT
    GGCTTACCCC AATCTGAGAA GAACAGAGTG CAGGACTGGC TTCACTTGGC AGAGGGCCTG
    AGGAGTCCGG TGGCAGCAGA TGCCGAGGGC AGTAGCAGAG AAGAGCCACA TCATGTCACC
    ATCTTGCAGC TGAAGAGGGA ACTGAAGAAA TGTCAGGATG CCCTAGCAAC AGATGAAGAG
    GTTTTCGCTA GGAAGAAGCT GGAACTGAAA GGGTTACAAG AGCAGATTCA GTCGCTACAG
    CAAGAAAACG AAGAATATCA GAAGTGCCTG CAAGAGGCAG AATATACTAA GACTTTGCAG
    ACTGAAAAAC TGGTGGAACA GCAGCTCCTT ATTGATGAAT TGAAGTCAAA GAAGCCGGAA
    ATCTACAGGC AGTTTTCGCA GGAAAATGAA GATGAAGAGG AACTTCCAAG GGAAATATCC
    CAAAGGCCAC ACACTGTGCC CCCCAGCACC CACACCCAGA AACTCTCGTC CAGTTCATTG
    CAGGCTGCAG GGCAGGCAGA GCCAAGGAAG GTACGAACAA GCCCCCCTTC CTATTCCCTA
    GAAAGAATGG TAGCGGGATT CCGGACTCGC AGCCAGATGC TCCTGGAGCT CATAGAGGAG
    CAGGATGAAG TTCTGCATGA GAAGTTCTCA GACCAAAGTG AAGAAGAACC TGATGAGCCA
    GAGAAAGAAA ACAGAAAGAA ATCACCATTT AGGCGGTCTA TCAATCGGAC ATGGACACGA
    CGACAAGGCT CTGCTGGTGC GGAAACACTT AACTCCAACT CAGACAGAGA GAATGACGCA
    AACTCTAATG CTTGTCCAGC TCTGGAATCC CTGCGAGAAA GTCAGGTGCT GAATGTGGAG
    AAACTGAAAA CCTCAGACGT TCGCCTCGCT GATGCCAAGC AGAAGATGAG AGAATTAACA
    ATAAACATCA AAATGAAGGA AGAACTCATT AAAGAGCTGA TTAAAACAGG TAACGATGCC
    CAATCAGTGA ACAGGCAGTA TTCCCTGAAG ATAGCCCATT TGGAAGGAGA AGCAGAGCAG
    GCCAAGTCAG AGCTGTCTGA AGCACAAAGG CAACTGCAGG AGTTGGAGAA CAAGGAATTA
    AGGGACGTGG CGCACAAAAC GAAACTGCAG AGAGATTTCC GTAAGAAAAT GGAAGAAGCA
    AAATTAAAAA TGCAGGCAGT GCAAACCAAA CAACAGGACA CCAGGAAGCT CGCCTCCCTC
    TCCGTCAGGA ACGAAAAGCG CGTGAGAGAA CTGGAAGTGA ATGTGGAGCA GATGCGCCAA
    CAGCAAGCCC AACTGCAGCA AAAGCTCAAA GCCGAAACTG AGATGAAGCG CAAATTAGAG
    GGCACCATTC AAAAGGAGCA AGAACGGATC AAAGAGCTGC AGCTCAAGAC CGAGCAGCAG
    AACAAGATAT TGAGGATGAA AAATGAAGAG ATTGCTGCAA TTAGAAGACG AAAGAGCGTT
    CCCAGTGTCT CTGATAACCT CCAACTTAAA CTGGAAGAAC AGACCAAGTG GCTTGATGAA
    GAGGTTGAAA AGGTACTGAA AGAAAGGCAA GCACTCGCCG AGCTGGAAGA GGATTTAAGG
    AAAAGGGAAG AAAGCGTCAC AAAGATGGAG GCGCTCTTGC AAGAAAAAAG CCACCTGGAG
    ATGAAGAAGC TTCGATCGAG CCAGGCTATA ACCAGTGACG TGCTAAAGCT GTCCAATCGC
    TTGAATGTTG TGGAGAATGA GCTCATGGAT AAAAGCCAAT TCCTGCAGAA CGACGCTAGT
    GATGAGCACA GGAAGGCATC TGAGGAGGTC AAAGAGCTGC AGAAGGAACG GGACGGTCTA
    CTAAAGAGAA GAGATAGCCT GGACGAGAAG CTGAGAAATG GCAGCGTTCT CTCCCCAGAG
    GAAGAGCACA CACTGTTCCA GCTGGAGGAG GGGATAGAAG CCTTGGAAGC TGCGATTGAA
    TATAAGAACG AATACATTCA GAACAGACAG AAGGTTCTGA AAGACTCCTC TCAGATTCTC
    ACCCAAAGTG AAGCCAACAT CATTGAAAAG TTTAGCTCTC TCTCGCCTTC AGAAACCCAA
    TCCATCCTTG TCCGATACTT CAACAAGGTC GTGAGTCTTC GTGAAGCAGA GCGGAAACTG
    CAGCTGCATT CGGCGGAACT GGCCATGAAA GTGACAGAGA AAGAGAACAT GCTACGAGAG
    CTGGAATCCT CCCTGGAACA TCTCATGCTT CAGAATGATC GAAGGCTGAC CCAACAGCAG
    AAGGAGCACG AGCAAAAGCT ACAGCTCCTC TTACAGCACT TTAAGGATAA CGACAATGAA
    GCAGTTCCAG AAAGCATCAA GGCTTACGAG GCAAAGGTGG CCGAGCTGGA GAAGGAGCTG
    TTCTTTTATA AGAAAACCAG CCGGGAGCTG AAAAAGAGGC TAAAAGAGCT GCTTGGGGAA
    CCCAAAGTGG CGGTTTTGAA AAGCAATAAC ACTGCTGGAG TCTTGGATGA ACCGGGTTCC
    CCGGCAGCCA AAGAATTGAA GTGGACCCCC CAGCTGGAAA GCAACAAAGC TGCCGGAAAA
    GCAAGAGAGT CCCAACATCC CGACCTTCAG CGCAATCCTT ACAGAGCCGC AGGGGATATT
    CCCGAAGGTG ACCCTACCTC CAGCTTCCCC AGGCTTCCCT CTAGAAGCCA TATCTCCAGA
    AGGGCCAGCT CAGAAGACGA GGAGCCGGGA CATATTCCCA CCCAGTCCAT ATCAAAGATG
    GAACCGAGCA AAAGTCTTTG TGTCACTCCG GTGAAAATAT CCCGAAGGGA ACTGCGGCAG
    ATCTCTGCGT CCCACCTATC GACCCGCCGC TCCAGCCTCA CCAGCACCCC CGCCGACTCC
    ATTGAAGTAG CAAGGACTGG AAAAGACTTT AAAGCTCAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.