KIF27 cDNA ORF clone, Bos taurus(cattle)

The following KIF27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIF27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa237176 NM_001206123.3
Latest version!
Bos taurus kinesin family member 27 (KIF27), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237176 XM_015472621.2
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237176 XM_005210336.4
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237176 XM_015472620.2
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237176 XM_015472619.2
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237177 XM_024995848.1
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237178 XM_015472622.2
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa237179 XM_015472623.2
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa91341 XM_015472622.1
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa91342 XM_015472623.1
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa22226 NM_001206123.2
Latest version!
Bos taurus kinesin family member 27 (KIF27), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa41053 NM_001206123.1
Latest version!
Bos taurus kinesin family member 27 (KIF27), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa22226 XM_015472620.1
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa22226 XM_005210336.3
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa22226 XM_015472621.1
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa22226 XM_015472619.1
Latest version!
Bos taurus kinesin family member 27 (KIF27), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa237176
    Clone ID Related Accession (Same CDS sequence) XM_015472621.2 , XM_005210336.4 , NM_001206123.3 , XM_015472619.2 , XM_015472620.2
    Accession Version NM_001206123.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000008.1. On May 29, 2018 this sequence version replaced NM_001206123.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571257.52820.1, SRR5721694.3580.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGTAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193052.2
    CDS1..4188
    Translation

    Target ORF information:

    RefSeq Version NM_001206123.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206123.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGTAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237176
    Clone ID Related Accession (Same CDS sequence) XM_015472621.2 , XM_005210336.4 , NM_001206123.3 , XM_015472619.2 , XM_015472620.2
    Accession Version XM_015472621.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGTAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328107.2
    CDS225..4412
    Translation

    Target ORF information:

    RefSeq Version XM_015472621.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472621.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGTAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237176
    Clone ID Related Accession (Same CDS sequence) XM_015472621.2 , XM_005210336.4 , NM_001206123.3 , XM_015472619.2 , XM_015472620.2
    Accession Version XM_005210336.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210336.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGTAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210393.2
    CDS256..4443
    Translation

    Target ORF information:

    RefSeq Version XM_005210336.4
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210336.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGTAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237176
    Clone ID Related Accession (Same CDS sequence) XM_015472621.2 , XM_005210336.4 , NM_001206123.3 , XM_015472619.2 , XM_015472620.2
    Accession Version XM_015472619.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGTAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328105.2
    CDS372..4559
    Translation

    Target ORF information:

    RefSeq Version XM_015472619.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472619.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGTAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237176
    Clone ID Related Accession (Same CDS sequence) XM_015472621.2 , XM_005210336.4 , NM_001206123.3 , XM_015472619.2 , XM_015472620.2
    Accession Version XM_015472620.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472620.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGTAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328106.2
    CDS349..4536
    Translation

    Target ORF information:

    RefSeq Version XM_015472620.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472620.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGTAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237177
    Clone ID Related Accession (Same CDS sequence) XM_024995848.1
    Accession Version XM_024995848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGAATGAAA AAATAATAGA ACAACAACTT CTTGTGGATC AACTGAGTGA AGAACTAACA
    AAACTTAACC TGTCAATGAC TTCTTCAGTA AAGGAAACTT GTGGAGATGG GCCAGATACC
    AGGATACTTG AAAAGAGACC ATACACTGTA CCATTTGATA CTCGTTTGGG GCATTATATT
    TATGGTCCAG TAAGGTTGGA TTCCAGGAAG GTCCACACAA GTCCTTCTGT ATACTCTCTG
    GATCGACTAG TTGCGGGATT TCGAACACGA AGTCAGATGC TTCTGGGTCG CTTAGAAGAA
    CAGGATGAAG TTCTCCAGTG CCAGTTTTCT GATAACAGTG ATGATGAAGA GTCAGAAGGC
    CAAGAGAAAT CGGAAATTAG ACATAGAAGT TGCTCATGGA TTCAGAAGCC AGGCTCTGTT
    TGTTCACTTG TTGAATTGAA TGATATTCAG GATCAAACAC AAAAGTCAGG ATTGGAGAAT
    GAAGATTTAA AGATTGAATG TCTCCAGGAG AGTCAAGAGT TAAATTTGCA AAAATTAAGA
    AATTCAGAAC TCATACTTAC TGAAGCTAAA CAAAAAATGA GAGAACTTAC TATTAACATC
    AAGATGAAGG AAGATCTGAT TAAAGAATTA ATTAAAACAG GTAATGATGC CAAGTCCGTA
    AGCAAGCAGT ATTCTCTGAA AGTAACAAAA CTTGAACATG AAGCAGAACA GGCAAAAGTG
    GAATTAACTG AAACAGAAAA GCAACTACAG GAACTGGAAA ACAAAGATCT TTCTGATGTT
    GCTTTGAAGG TAAAATTACA GAAGGAATTT CGTAGAAAGA TGGATGCCGC TAAGCTAAGA
    GTCCAGGTCT TACAGAAGAA GCATCAAAAT AGTAAGAAAT TGGCATCACT GTCAATCCAA
    AATGAGAAAC GTGCTAATGA ACTAGAGCAG AATGTAGATC ACATGAAATA TCAAAAGGTA
    CAGCTGCAAA AAAGACTTCG AGAAGAAAAC GAAAAAAGGA AACAACTGGA TGCAGAAATT
    AAGCGGGATC AACAAAAAAT CAAAGAACTA CAGTTAAAAA CAGAACAGAA AGATCTAAAA
    CTGAAAGCTG AGGACCTTGA TGCATTTAAG ATGAAAAGGA GAAAAGGTTC GTTTGGAAGT
    ATAGACCAAC TCCAGAAATT GGATAAGCAA AAGAAATGGT TAGATGAAGA AGTAGAGAAA
    GTTCTGAACC AACGCCAAGA ATTAGAGGAG CTGGAAGAAG ACTTAAAGAA ACGGGAGGCC
    ATAGTTTCAA AGAAGGAGGC CCTGTTACAG GAGAAGAGCC ACCTGGAAAG TAAGAAGTTG
    CGATCTAGTC AGGCTTTGAA CACAGACAGT TTAAAAATAT CAACTCGCCT GAGCTTGTTG
    GACCAAGAGT TGTCTGAAAA GAATGTGCAG CTCCAGAGCA GCACAGCTGA GGAGAAAATA
    AAGATTTCAG AACAAGTACA AGCCCTCCAG AGAGAAAAGG ATCAGCTGCA GAGACAAAGA
    AACAGCGTGG ATGAGAAACT TAAAAATGGT AGCGTGTTGT CACCTGAAGA AGAACATGTT
    CTTTTCCAAC TTGAAGAAGG GATTGAAGCT TTGGAAGCCG CAATTGAATA CAAGAACGAA
    AGTATCCAGA GTCGCCAAAA CTTGCTCAGA GCTTCATTTC AAAACCTCTC TCATAGTGAA
    GCAAATGTCT TAGAAAAACT AATTTGCCTG AGTCCTGTTG AGATTAGAGC TGTTCTTTTC
    AGATATTTCA ATAAGGTGGT TAATTTGCGA GAAGTTGAAC GGAAACTAAA GTTACAGAAT
    GAAGAAATTA AAATGAAAGT TCTGGAACGG GATAACATGG TTCGTGAGTT AGAATCTGCA
    CTGGAACATC TGAAATTTCA GTGTGACCGG AGACTGATCC TCCAGCAAAA GGAACATGAG
    CAAAAAATGC AACTGCTGTT ACATCATTTC AAAGAGCGAG ATGGGGAAGG CATTGTGGAA
    ACTTTAAAAA ATTATGAAGA TAAAATCCAT CAGCTGGAAA GAGATCTATA TTTCTATAAG
    AAAACCAGCA GAGATCTGAA GAAGAAACTC AAGGAACTGG TAGGGGAAGC AATTCGGCGG
    CAACTACTAC CATCAGAAAA TCATGATGCT GGAGATGGAG TTCAGAACCC AGAAAAGGCC
    GGCATGGTTT CAGAAGAATT AAAATGGGCA TCCAGAACTG AAAGTATGAA ATTAAGTGGA
    AGGGAAAGAC CATTAGACAG TTCCACAAGC AGTTTAAGAA CACAACCAAA TCCTCAGAAG
    CTCTGGGAAG ATGGCCCAGA ATTAGCTCCA ATGTATAGCT CTGTAGCACC CACTAGTGGG
    CATTTGTTAA GCAATGAGGA CAAAACCGAA ATAGATGAAA ATCAGTTTAC AAAATCTAAC
    AGTCGACCAT CATCCCACAT TCAGCCAGTC GGAAATGTGG GACAGCTGCA TGGTGTCACA
    CCTGTAAAGT TGTGTCGCAA AGAGTTACGC CAGATTTCTG CCTTGGAACT ATCATTACGA
    CGTTCCAATC TTGGAGTTGC GGTCGGATCT ATGACTGCTG ATTCCCTTGA AGTAGCTAGG
    AAAGTGAGTG ACTTAAAAAC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851616.1
    CDS218..4261
    Translation

    Target ORF information:

    RefSeq Version XM_024995848.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGAATGAAA AAATAATAGA ACAACAACTT CTTGTGGATC AACTGAGTGA AGAACTAACA
    AAACTTAACC TGTCAATGAC TTCTTCAGTA AAGGAAACTT GTGGAGATGG GCCAGATACC
    AGGATACTTG AAAAGAGACC ATACACTGTA CCATTTGATA CTCGTTTGGG GCATTATATT
    TATGGTCCAG TAAGGTTGGA TTCCAGGAAG GTCCACACAA GTCCTTCTGT ATACTCTCTG
    GATCGACTAG TTGCGGGATT TCGAACACGA AGTCAGATGC TTCTGGGTCG CTTAGAAGAA
    CAGGATGAAG TTCTCCAGTG CCAGTTTTCT GATAACAGTG ATGATGAAGA GTCAGAAGGC
    CAAGAGAAAT CGGAAATTAG ACATAGAAGT TGCTCATGGA TTCAGAAGCC AGGCTCTGTT
    TGTTCACTTG TTGAATTGAA TGATATTCAG GATCAAACAC AAAAGTCAGG ATTGGAGAAT
    GAAGATTTAA AGATTGAATG TCTCCAGGAG AGTCAAGAGT TAAATTTGCA AAAATTAAGA
    AATTCAGAAC TCATACTTAC TGAAGCTAAA CAAAAAATGA GAGAACTTAC TATTAACATC
    AAGATGAAGG AAGATCTGAT TAAAGAATTA ATTAAAACAG GTAATGATGC CAAGTCCGTA
    AGCAAGCAGT ATTCTCTGAA AGTAACAAAA CTTGAACATG AAGCAGAACA GGCAAAAGTG
    GAATTAACTG AAACAGAAAA GCAACTACAG GAACTGGAAA ACAAAGATCT TTCTGATGTT
    GCTTTGAAGG TAAAATTACA GAAGGAATTT CGTAGAAAGA TGGATGCCGC TAAGCTAAGA
    GTCCAGGTCT TACAGAAGAA GCATCAAAAT AGTAAGAAAT TGGCATCACT GTCAATCCAA
    AATGAGAAAC GTGCTAATGA ACTAGAGCAG AATGTAGATC ACATGAAATA TCAAAAGGTA
    CAGCTGCAAA AAAGACTTCG AGAAGAAAAC GAAAAAAGGA AACAACTGGA TGCAGAAATT
    AAGCGGGATC AACAAAAAAT CAAAGAACTA CAGTTAAAAA CAGAACAGAA AGATCTAAAA
    CTGAAAGCTG AGGACCTTGA TGCATTTAAG ATGAAAAGGA GAAAAGGTTC GTTTGGAAGT
    ATAGACCAAC TCCAGAAATT GGATAAGCAA AAGAAATGGT TAGATGAAGA AGTAGAGAAA
    GTTCTGAACC AACGCCAAGA ATTAGAGGAG CTGGAAGAAG ACTTAAAGAA ACGGGAGGCC
    ATAGTTTCAA AGAAGGAGGC CCTGTTACAG GAGAAGAGCC ACCTGGAAAG TAAGAAGTTG
    CGATCTAGTC AGGCTTTGAA CACAGACAGT TTAAAAATAT CAACTCGCCT GAGCTTGTTG
    GACCAAGAGT TGTCTGAAAA GAATGTGCAG CTCCAGAGCA GCACAGCTGA GGAGAAAATA
    AAGATTTCAG AACAAGTACA AGCCCTCCAG AGAGAAAAGG ATCAGCTGCA GAGACAAAGA
    AACAGCGTGG ATGAGAAACT TAAAAATGGT AGCGTGTTGT CACCTGAAGA AGAACATGTT
    CTTTTCCAAC TTGAAGAAGG GATTGAAGCT TTGGAAGCCG CAATTGAATA CAAGAACGAA
    AGTATCCAGA GTCGCCAAAA CTTGCTCAGA GCTTCATTTC AAAACCTCTC TCATAGTGAA
    GCAAATGTCT TAGAAAAACT AATTTGCCTG AGTCCTGTTG AGATTAGAGC TGTTCTTTTC
    AGATATTTCA ATAAGGTGGT TAATTTGCGA GAAGTTGAAC GGAAACTAAA GTTACAGAAT
    GAAGAAATTA AAATGAAAGT TCTGGAACGG GATAACATGG TTCGTGAGTT AGAATCTGCA
    CTGGAACATC TGAAATTTCA GTGTGACCGG AGACTGATCC TCCAGCAAAA GGAACATGAG
    CAAAAAATGC AACTGCTGTT ACATCATTTC AAAGAGCGAG ATGGGGAAGG CATTGTGGAA
    ACTTTAAAAA ATTATGAAGA TAAAATCCAT CAGCTGGAAA GAGATCTATA TTTCTATAAG
    AAAACCAGCA GAGATCTGAA GAAGAAACTC AAGGAACTGG TAGGGGAAGC AATTCGGCGG
    CAACTACTAC CATCAGAAAA TCATGATGCT GGAGATGGAG TTCAGAACCC AGAAAAGGCC
    GGCATGGTTT CAGAAGAATT AAAATGGGCA TCCAGAACTG AAAGTATGAA ATTAAGTGGA
    AGGGAAAGAC CATTAGACAG TTCCACAAGC AGTTTAAGAA CACAACCAAA TCCTCAGAAG
    CTCTGGGAAG ATGGCCCAGA ATTAGCTCCA ATGTATAGCT CTGTAGCACC CACTAGTGGG
    CATTTGTTAA GCAATGAGGA CAAAACCGAA ATAGATGAAA ATCAGTTTAC AAAATCTAAC
    AGTCGACCAT CATCCCACAT TCAGCCAGTC GGAAATGTGG GACAGCTGCA TGGTGTCACA
    CCTGTAAAGT TGTGTCGCAA AGAGTTACGC CAGATTTCTG CCTTGGAACT ATCATTACGA
    CGTTCCAATC TTGGAGTTGC GGTCGGATCT ATGACTGCTG ATTCCCTTGA AGTAGCTAGG
    AAAGTGAGTG ACTTAAAAAC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237178
    Clone ID Related Accession (Same CDS sequence) XM_015472622.2
    Accession Version XM_015472622.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGTG
    ATTGTTGGGG CCAAGGAATG CCAGGTGGAG AGTGCAGATG AAGTGATGAG TCTCCTGGAG
    ATGGGAAATG CAGCCAGGCA TACGGGTACG ACCCAAATGA ATGAGCACTC CAGTAGATCA
    CACGCGATTT TCACCATCAG CCTTTGTCAA GTGGACAAAA ATAGAAAGAC AGCTGAAGAT
    GGATCATGGC ATTCCCATCG GCATATTGTC TCAAAGTTCC ACTTTGTGGA TTTGGCTGGA
    TCAGAAAGAG TCACCAAAAC GGGGAATACC GGCGAACGTT TCAAAGAATC CATTCAGATC
    AACAGTGGGT TGCTGGCTTT AGGAAATGTA ATAAGCGCTC TTGGGGACCC ACGAAAGAAG
    AGTTCACATA TTCCATACAG GGATGCTAAA ATTACCCGGC TTCTGAAAGA CTCCCTGGGA
    GGCAGTGCCA AGACTGTCAT GATCACTTGT GTCAGCCCAT CCTCCTCAGA TTTTGACGAG
    TCCTTAAATT CCCTCAAATA TGCCAACAGA GCACGCAACA TTAGAAACAA ACCCACCTTA
    AACTTTAGCC CCGAGTCAGA CCGTATGGAC GAAATGGAAT TTGAGATTAA GTTGCTTCGA
    GAAGCTTTGC AAAGCCAGCA GGCTAGTAGC AGCCAAACTA GCCAGATCCA TCAGGAGGGG
    ACTCCTGATA AAAATAAGAT CCATTCTCTT GAGGAGCAAG TCACTCAGCT ACAAGGAGAA
    TGTCTGGGTT ACCAAAACTG TTTAGAGGAA GCCTTTACCT TCCTGGTGGA TTTAAAAGAT
    TCTGTGAGAC TAAATGAAAA GCAGCAACAC AAACTGCAGG AATGGTTTAA CATGACCCAG
    GAGGTCACCT TGTTCAGAGG ACACAGGGGC ATTGGAAACC TGGAAGAAGG ACCACAGCAT
    ATTACAGTTC TCCAGCTGAA GCGAGAGCTT AAGAAATGCC AGTGTGCTCT TGCTGCTGAT
    GAAGTAGTAT TTAATCAGAA GGACTTGGAG GTGAAGGAGC TAAAGAATCA AGTGCAAATG
    ATGGTGCAGG AAAACAAAGG ACATGTTGTT TCTTTGAAAG AAGCACAAAA AGTGAATAGA
    TTGCAGAATG AAAAAATAAT AGAACAACAA CTTCTTGTGG ATCAACTGAG TGAAGAACTA
    ACAAAACTTA ACCTGTCAAT GACTTCTTCA GTAAAGGAAA CTTGTGGAGA TGGGCCAGAT
    ACCAGGATAC TTGAAAAGAG ACCATACACT GTACCATTTG ATACTCGTTT GGGGCATTAT
    ATTTATGGTC CAGTAAGGTT GGATTCCAGG AAGGTCCACA CAAGTCCTTC TGTATACTCT
    CTGGATCGAC TAGTTGCGGG ATTTCGAACA CGAAGTCAGA TGCTTCTGGG TCGCTTAGAA
    GAACAGGATG AAGTTCTCCA GTGCCAGTTT TCTGATAACA GTGATGATGA AGAGTCAGAA
    GGCCAAGAGA AATCGGAAAT TAGACATAGA AGTTGCTCAT GGATTCAGAA GCCAGGCTCT
    GTTTGTTCAC TTGTTGAATT GAATGATATT CAGGATCAAA CACAAAAGTC AGGATTGGAG
    AATGAAGATT TAAAGATTGA ATGTCTCCAG GAGAGTCAAG AGTTAAATTT GCAAAAATTA
    AGAAATTCAG AACTCATACT TACTGAAGCT AAACAAAAAA TGAGAGAACT TACTATTAAC
    ATCAAGATGA AGGAAGATCT GATTAAAGAA TTAATTAAAA CAGGTAATGA TGCCAAGTCC
    GTAAGCAAGC AGTATTCTCT GAAAGTAACA AAACTTGAAC ATGAAGCAGA ACAGGCAAAA
    GTGGAATTAA CTGAAACAGA AAAGCAACTA CAGGAACTGG AAAACAAAGA TCTTTCTGAT
    GTTGCTTTGA AGGTAAAATT ACAGAAGGAA TTTCGTAGAA AGATGGATGC CGCTAAGCTA
    AGAGTCCAGG TCTTACAGAA GAAGCATCAA AATAGTAAGA AATTGGCATC ACTGTCAATC
    CAAAATGAGA AACGTGCTAA TGAACTAGAG CAGAATGTAG ATCACATGAA ATATCAAAAG
    GTACAGCTGC AAAAAAGACT TCGAGAAGAA AACGAAAAAA GGAAACAACT GGATGCAGAA
    ATTAAGCGGG ATCAACAAAA AATCAAAGAA CTACAGTTAA AAACAGAACA GAAAGATCTA
    AAACTGAAAG CTGAGGACCT TGATGCATTT AAGATGAAAA GGAGAAAAGG TTCGTTTGGA
    AGTATAGACC AACTCCAGAA ATTGGATAAG CAAAAGAAAT GGTTAGATGA AGAAGTAGAG
    AAAGTTCTGA ACCAACGCCA AGAATTAGAG GAGCTGGAAG AAGACTTAAA GAAACGGGAG
    GCCATAGTTT CAAAGAAGGA GGCCCTGTTA CAGGAGAAGA GCCACCTGGA AAGTAAGAAG
    TTGCGATCTA GTCAGGCTTT GAACACAGAC AGTTTAAAAA TATCAACTCG CCTGAGCTTG
    TTGGACCAAG AGTTGTCTGA AAAGAATGTG CAGCTCCAGA GCAGCACAGC TGAGGAGAAA
    ATAAAGATTT CAGAACAAGT ACAAGCCCTC CAGAGAGAAA AGGATCAGCT GCAGAGACAA
    AGAAACAGCG TGGATGAGAA ACTTAAAAAT GGTAGCGTGT TGTCACCTGA AGAAGAACAT
    GTTCTTTTCC AACTTGAAGA AGGGATTGAA GCTTTGGAAG CCGCAATTGA ATACAAGAAC
    GAAAGTATCC AGAGTCGCCA AAACTTGCTC AGAGCTTCAT TTCAAAACCT CTCTCATAGT
    GAAGCAAATG TCTTAGAAAA ACTAATTTGC CTGAGTCCTG TTGAGATTAG AGCTGTTCTT
    TTCAGATATT TCAATAAGGT GGTTAATTTG CGAGAAGTTG AACGGAAACT AAAGTTACAG
    AATGAAGAAA TTAAAATGAA AGTTCTGGAA CGGGATAACA TGGTTCGTGA GTTAGAATCT
    GCACTGGAAC ATCTGAAATT TCAGTGTGAC CGGAGACTGA TCCTCCAGCA AAAGGAACAT
    GAGCAAAAAA TGCAACTGCT GTTACATCAT TTCAAAGAGC GAGATGGGGA AGGCATTGTG
    GAAACTTTAA AAAATTATGA AGATAAAATC CATCAGCTGG AAAGAGATCT ATATTTCTAT
    AAGAAAACCA GCAGAGATCT GAAGAAGAAA CTCAAGGAAC TGGTAGGGGA AGCAATTCGG
    CGGCAACTAC TACCATCAGA AAATCATGAT GCTGGAGATG GAGTTCAGAA CCCAGAAAAG
    GCCGGCATGG TTTCAGAAGA ATTAAAATGG GCATCCAGAA CTGAAAGTAT GAAATTAAGT
    GGAAGGGAAA GACCATTAGA CAGTTCCACA AGCAGTTTAA GAACACAACC AAATCCTCAG
    AAGCTCTGGG AAGATGGCCC AGAATTAGCT CCAATGTATA GCTCTGTAGC ACCCACTAGT
    GGGCATTTGT TAAGCAATGA GGACAAAACC GAAATAGATG AAAATCAGTT TACAAAATCT
    AACAGTCGAC CATCATCCCA CATTCAGCCA GTCGGAAATG TGGGACAGCT GCATGGTGTC
    ACACCTGTAA AGTTGTGTCG CAAAGAGTTA CGCCAGATTT CTGCCTTGGA ACTATCATTA
    CGACGTTCCA ATCTTGGAGT TGCGGTCGGA TCTATGACTG CTGATTCCCT TGAAGTAGCT
    AGGAAAGTGA GTGACTTAAA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328108.2
    CDS218..4204
    Translation

    Target ORF information:

    RefSeq Version XM_015472622.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472622.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGTG
    ATTGTTGGGG CCAAGGAATG CCAGGTGGAG AGTGCAGATG AAGTGATGAG TCTCCTGGAG
    ATGGGAAATG CAGCCAGGCA TACGGGTACG ACCCAAATGA ATGAGCACTC CAGTAGATCA
    CACGCGATTT TCACCATCAG CCTTTGTCAA GTGGACAAAA ATAGAAAGAC AGCTGAAGAT
    GGATCATGGC ATTCCCATCG GCATATTGTC TCAAAGTTCC ACTTTGTGGA TTTGGCTGGA
    TCAGAAAGAG TCACCAAAAC GGGGAATACC GGCGAACGTT TCAAAGAATC CATTCAGATC
    AACAGTGGGT TGCTGGCTTT AGGAAATGTA ATAAGCGCTC TTGGGGACCC ACGAAAGAAG
    AGTTCACATA TTCCATACAG GGATGCTAAA ATTACCCGGC TTCTGAAAGA CTCCCTGGGA
    GGCAGTGCCA AGACTGTCAT GATCACTTGT GTCAGCCCAT CCTCCTCAGA TTTTGACGAG
    TCCTTAAATT CCCTCAAATA TGCCAACAGA GCACGCAACA TTAGAAACAA ACCCACCTTA
    AACTTTAGCC CCGAGTCAGA CCGTATGGAC GAAATGGAAT TTGAGATTAA GTTGCTTCGA
    GAAGCTTTGC AAAGCCAGCA GGCTAGTAGC AGCCAAACTA GCCAGATCCA TCAGGAGGGG
    ACTCCTGATA AAAATAAGAT CCATTCTCTT GAGGAGCAAG TCACTCAGCT ACAAGGAGAA
    TGTCTGGGTT ACCAAAACTG TTTAGAGGAA GCCTTTACCT TCCTGGTGGA TTTAAAAGAT
    TCTGTGAGAC TAAATGAAAA GCAGCAACAC AAACTGCAGG AATGGTTTAA CATGACCCAG
    GAGGTCACCT TGTTCAGAGG ACACAGGGGC ATTGGAAACC TGGAAGAAGG ACCACAGCAT
    ATTACAGTTC TCCAGCTGAA GCGAGAGCTT AAGAAATGCC AGTGTGCTCT TGCTGCTGAT
    GAAGTAGTAT TTAATCAGAA GGACTTGGAG GTGAAGGAGC TAAAGAATCA AGTGCAAATG
    ATGGTGCAGG AAAACAAAGG ACATGTTGTT TCTTTGAAAG AAGCACAAAA AGTGAATAGA
    TTGCAGAATG AAAAAATAAT AGAACAACAA CTTCTTGTGG ATCAACTGAG TGAAGAACTA
    ACAAAACTTA ACCTGTCAAT GACTTCTTCA GTAAAGGAAA CTTGTGGAGA TGGGCCAGAT
    ACCAGGATAC TTGAAAAGAG ACCATACACT GTACCATTTG ATACTCGTTT GGGGCATTAT
    ATTTATGGTC CAGTAAGGTT GGATTCCAGG AAGGTCCACA CAAGTCCTTC TGTATACTCT
    CTGGATCGAC TAGTTGCGGG ATTTCGAACA CGAAGTCAGA TGCTTCTGGG TCGCTTAGAA
    GAACAGGATG AAGTTCTCCA GTGCCAGTTT TCTGATAACA GTGATGATGA AGAGTCAGAA
    GGCCAAGAGA AATCGGAAAT TAGACATAGA AGTTGCTCAT GGATTCAGAA GCCAGGCTCT
    GTTTGTTCAC TTGTTGAATT GAATGATATT CAGGATCAAA CACAAAAGTC AGGATTGGAG
    AATGAAGATT TAAAGATTGA ATGTCTCCAG GAGAGTCAAG AGTTAAATTT GCAAAAATTA
    AGAAATTCAG AACTCATACT TACTGAAGCT AAACAAAAAA TGAGAGAACT TACTATTAAC
    ATCAAGATGA AGGAAGATCT GATTAAAGAA TTAATTAAAA CAGGTAATGA TGCCAAGTCC
    GTAAGCAAGC AGTATTCTCT GAAAGTAACA AAACTTGAAC ATGAAGCAGA ACAGGCAAAA
    GTGGAATTAA CTGAAACAGA AAAGCAACTA CAGGAACTGG AAAACAAAGA TCTTTCTGAT
    GTTGCTTTGA AGGTAAAATT ACAGAAGGAA TTTCGTAGAA AGATGGATGC CGCTAAGCTA
    AGAGTCCAGG TCTTACAGAA GAAGCATCAA AATAGTAAGA AATTGGCATC ACTGTCAATC
    CAAAATGAGA AACGTGCTAA TGAACTAGAG CAGAATGTAG ATCACATGAA ATATCAAAAG
    GTACAGCTGC AAAAAAGACT TCGAGAAGAA AACGAAAAAA GGAAACAACT GGATGCAGAA
    ATTAAGCGGG ATCAACAAAA AATCAAAGAA CTACAGTTAA AAACAGAACA GAAAGATCTA
    AAACTGAAAG CTGAGGACCT TGATGCATTT AAGATGAAAA GGAGAAAAGG TTCGTTTGGA
    AGTATAGACC AACTCCAGAA ATTGGATAAG CAAAAGAAAT GGTTAGATGA AGAAGTAGAG
    AAAGTTCTGA ACCAACGCCA AGAATTAGAG GAGCTGGAAG AAGACTTAAA GAAACGGGAG
    GCCATAGTTT CAAAGAAGGA GGCCCTGTTA CAGGAGAAGA GCCACCTGGA AAGTAAGAAG
    TTGCGATCTA GTCAGGCTTT GAACACAGAC AGTTTAAAAA TATCAACTCG CCTGAGCTTG
    TTGGACCAAG AGTTGTCTGA AAAGAATGTG CAGCTCCAGA GCAGCACAGC TGAGGAGAAA
    ATAAAGATTT CAGAACAAGT ACAAGCCCTC CAGAGAGAAA AGGATCAGCT GCAGAGACAA
    AGAAACAGCG TGGATGAGAA ACTTAAAAAT GGTAGCGTGT TGTCACCTGA AGAAGAACAT
    GTTCTTTTCC AACTTGAAGA AGGGATTGAA GCTTTGGAAG CCGCAATTGA ATACAAGAAC
    GAAAGTATCC AGAGTCGCCA AAACTTGCTC AGAGCTTCAT TTCAAAACCT CTCTCATAGT
    GAAGCAAATG TCTTAGAAAA ACTAATTTGC CTGAGTCCTG TTGAGATTAG AGCTGTTCTT
    TTCAGATATT TCAATAAGGT GGTTAATTTG CGAGAAGTTG AACGGAAACT AAAGTTACAG
    AATGAAGAAA TTAAAATGAA AGTTCTGGAA CGGGATAACA TGGTTCGTGA GTTAGAATCT
    GCACTGGAAC ATCTGAAATT TCAGTGTGAC CGGAGACTGA TCCTCCAGCA AAAGGAACAT
    GAGCAAAAAA TGCAACTGCT GTTACATCAT TTCAAAGAGC GAGATGGGGA AGGCATTGTG
    GAAACTTTAA AAAATTATGA AGATAAAATC CATCAGCTGG AAAGAGATCT ATATTTCTAT
    AAGAAAACCA GCAGAGATCT GAAGAAGAAA CTCAAGGAAC TGGTAGGGGA AGCAATTCGG
    CGGCAACTAC TACCATCAGA AAATCATGAT GCTGGAGATG GAGTTCAGAA CCCAGAAAAG
    GCCGGCATGG TTTCAGAAGA ATTAAAATGG GCATCCAGAA CTGAAAGTAT GAAATTAAGT
    GGAAGGGAAA GACCATTAGA CAGTTCCACA AGCAGTTTAA GAACACAACC AAATCCTCAG
    AAGCTCTGGG AAGATGGCCC AGAATTAGCT CCAATGTATA GCTCTGTAGC ACCCACTAGT
    GGGCATTTGT TAAGCAATGA GGACAAAACC GAAATAGATG AAAATCAGTT TACAAAATCT
    AACAGTCGAC CATCATCCCA CATTCAGCCA GTCGGAAATG TGGGACAGCT GCATGGTGTC
    ACACCTGTAA AGTTGTGTCG CAAAGAGTTA CGCCAGATTT CTGCCTTGGA ACTATCATTA
    CGACGTTCCA ATCTTGGAGT TGCGGTCGGA TCTATGACTG CTGATTCCCT TGAAGTAGCT
    AGGAAAGTGA GTGACTTAAA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237179
    Clone ID Related Accession (Same CDS sequence) XM_015472623.2
    Accession Version XM_015472623.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472623.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGTTCCTG GGGTCCTAGA ATACTGCCTG GCACATACTT CAGTTGTGGA GGGTCAAAAA 
    GGTATTATTC CTCGTGCTAT CCAAGAAATA TTTCAAAACA TCTCTGGAAA GCCTAGCATT
    GACTTTAATA TAAAAGTATC TTATATAGAA GTATACAAGG AAGACCTAAG AGATCTTCTA
    GAATTGGAGA CATCAGTAAA AGATCTTCAC ATCCGAGAAG ATGAAAAAGG AAACACAGTG
    ATTGTTGGGG CCAAGGAATG CCAGGTGGAG AGTGCAGATG AAGTGATGAG TCTCCTGGAG
    ATGGGAAATG CAGCCAGGCA TACGGGTACG ACCCAAATGA ATGAGCACTC CAGTAGATCA
    CACGCGATTT TCACCATCAG CCTTTGTCAA GTGGACAAAA ATAGAAAGAC AGCTGAAGAT
    GGATCATGGC ATTCCCATCG GCATATTGTC TCAAAGTTCC ACTTTGTGGA TTTGGCTGGA
    TCAGAAAGAG TCACCAAAAC GGGGAATACC GGCGAACGTT TCAAAGAATC CATTCAGATC
    AACAGTGGGT TGCTGGCTTT AGGAAATGTA ATAAGCGCTC TTGGGGACCC ACGAAAGAAG
    AGTTCACATA TTCCATACAG GGATGCTAAA ATTACCCGGC TTCTGAAAGA CTCCCTGGGA
    GGCAGTGCCA AGACTGTCAT GATCACTTGT GTCAGCCCAT CCTCCTCAGA TTTTGACGAG
    TCCTTAAATT CCCTCAAATA TGCCAACAGA GCACGCAACA TTAGAAACAA ACCCACCTTA
    AACTTTAGCC CCGAGTCAGA CCGTATGGAC GAAATGGAAT TTGAGATTAA GTTGCTTCGA
    GAAGCTTTGC AAAGCCAGCA GGCTAGTAGC AGCCAAACTA GCCAGATCCA TCAGGAGGGG
    ACTCCTGATA AAAATAAGAT CCATTCTCTT GAGGAGCAAG TCACTCAGCT ACAAGGAGAA
    TGTCTGGGTT ACCAAAACTG TTTAGAGGAA GCCTTTACCT TCCTGGTGGA TTTAAAAGAT
    TCTGTGAGAC TAAATGAAAA GCAGCAACAC AAACTGCAGG AATGGTTTAA CATGACCCAG
    GAGGTCACCT TGTTCAGAGG ACACAGGGGC ATTGGAAACC TGGAAGAAGG ACCACAGCAT
    ATTACAGTTC TCCAGCTGAA GCGAGAGCTT AAGAAATGCC AGTGTGCTCT TGCTGCTGAT
    GAAGTAGTAT TTAATCAGAA GGACTTGGAG GTGAAGGAGC TAAAGAATCA AGTGCAAATG
    ATGGTGCAGG AAAACAAAGG ACATGTTGTT TCTTTGAAAG AAGCACAAAA AGTGAATAGA
    TTGCAGAATG AAAAAATAAT AGAACAACAA CTTCTTGTGG ATCAACTGAG TGAAGAACTA
    ACAAAACTTA ACCTGTCAAT GACTTCTTCA GTAAAGGAAA CTTGTGGAGA TGGGCCAGAT
    ACCAGGATAC TTGAAAAGAG ACCATACACT GTACCATTTG ATACTCGTTT GGGGCATTAT
    ATTTATGGTC CAGTAAGGTT GGATTCCAGG AAGGTCCACA CAAGTCCTTC TGTATACTCT
    CTGGATCGAC TAGTTGCGGG ATTTCGAACA CGAAGTCAGA TGCTTCTGGG TCGCTTAGAA
    GAACAGGATG AAGTTCTCCA GTGCCAGTTT TCTGATAACA GTGATGATGA AGAGTCAGAA
    GGCCAAGAGA AATCGGAAAT TAGACATAGA AGTTGCTCAT GGATTCAGAA GCCAGGCTCT
    GTTTGTTCAC TTGTTGAATT GAATGATATT CAGGATCAAA CACAAAAGTC AGGATTGGAG
    AATGAAGATT TAAAGATTGA ATGTCTCCAG GAGAGTCAAG AGTTAAATTT GCAAAAATTA
    AGAAATTCAG AACTCATACT TACTGAAGCT AAACAAAAAA TGAGAGAACT TACTATTAAC
    ATCAAGATGA AGGAAGATCT GATTAAAGAA TTAATTAAAA CAGGTAATGA TGCCAAGTCC
    GTAAGCAAGC AGTATTCTCT GAAAGTAACA AAACTTGAAC ATGAAGCAGA ACAGGCAAAA
    GTGGAATTAA CTGAAACAGA AAAGCAACTA CAGGAACTGG AAAACAAAGA TCTTTCTGAT
    GTTGCTTTGA AGGTAAAATT ACAGAAGGAA TTTCGTAGAA AGATGGATGC CGCTAAGCTA
    AGAGTCCAGG TCTTACAGAA GAAGCATCAA AATAGTAAGA AATTGGCATC ACTGTCAATC
    CAAAATGAGA AACGTGCTAA TGAACTAGAG CAGAATGTAG ATCACATGAA ATATCAAAAG
    GTACAGCTGC AAAAAAGACT TCGAGAAGAA AACGAAAAAA GGAAACAACT GGATGCAGAA
    ATTAAGCGGG ATCAACAAAA AATCAAAGAA CTACAGTTAA AAACAGAACA GAAAGATCTA
    AAACTGAAAG CTGAGGACCT TGATGCATTT AAGATGAAAA GGAGAAAAGG TTCGTTTGGA
    AGTATAGACC AACTCCAGAA ATTGGATAAG CAAAAGAAAT GGTTAGATGA AGAAGTAGAG
    AAAGTTCTGA ACCAACGCCA AGAATTAGAG GAGCTGGAAG AAGACTTAAA GAAACGGGAG
    GCCATAGTTT CAAAGAAGGA GGCCCTGTTA CAGGAGAAGA GCCACCTGGA AAGTAAGAAG
    TTGCGATCTA GTCAGGCTTT GAACACAGAC AGTTTAAAAA TATCAACTCG CCTGAGCTTG
    TTGGACCAAG AGTTGTCTGA AAAGAATGTG CAGCTCCAGA GCAGCACAGC TGAGGAGAAA
    ATAAAGATTT CAGAACAAGT ACAAGCCCTC CAGAGAGAAA AGGATCAGCT GCAGAGACAA
    AGAAACAGCG TGGATGAGAA ACTTAAAAAT GGTAGCGTGT TGTCACCTGA AGAAGAACAT
    GTTCTTTTCC AACTTGAAGA AGGGATTGAA GCTTTGGAAG CCGCAATTGA ATACAAGAAC
    GAAAGTATCC AGAGTCGCCA AAACTTGCTC AGAGCTTCAT TTCAAAACCT CTCTCATAGT
    GAAGCAAATG TCTTAGAAAA ACTAATTTGC CTGAGTCCTG TTGAGATTAG AGCTGTTCTT
    TTCAGATATT TCAATAAGGT GGTTAATTTG CGAGAAGTTG AACGGAAACT AAAGTTACAG
    AATGAAGAAA TTAAAATGAA AGTTCTGGAA CGGGATAACA TGGTTCGTGA GTTAGAATCT
    GCACTGGAAC ATCTGAAATT TCAGTGTGAC CGGAGACTGA TCCTCCAGCA AAAGGAACAT
    GAGCAAAAAA TGCAACTGCT GTTACATCAT TTCAAAGAGC GAGATGGGGA AGGCATTGTG
    GAAACTTTAA AAAATTATGA AGATAAAATC CATCAGCTGG AAAGAGATCT ATATTTCTAT
    AAGAAAACCA GCAGAGATCT GAAGAAGAAA CTCAAGGAAC TGGTAGGGGA AGCAATTCGG
    CGGCAACTAC TACCATCAGA AAATCATGAT GCTGGAGATG GAGTTCAGAA CCCAGAAAAG
    GCCGGCATGG TTTCAGAAGA ATTAAAATGG GCATCCAGAA CTGAAAGTAT GAAATTAAGT
    GGAAGGGAAA GACCATTAGA CAGTTCCACA AGCAGTTTAA GAACACAACC AAATCCTCAG
    AAGCTCTGGG AAGATGGCCC AGAATTAGCT CCAATGTATA GCTCTGTAGC ACCCACTAGT
    GGGCATTTGT TAAGCAATGA GGACAAAACC GAAATAGATG AAAATCAGTT TACAAAATCT
    AACAGTCGAC CATCATCCCA CATTCAGCCA GTCGGAAATG TGGGACAGCT GCATGGTGTC
    ACACCTGTAA AGTTGTGTCG CAAAGAGTTA CGCCAGATTT CTGCCTTGGA ACTATCATTA
    CGACGTTCCA ATCTTGGAGT TGCGGTCGGA TCTATGACTG CTGATTCCCT TGAAGTAGCT
    AGGAAAGTGA GTGACTTAAA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328109.2
    CDS1543..5469
    Translation

    Target ORF information:

    RefSeq Version XM_015472623.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472623.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGTTCCTG GGGTCCTAGA ATACTGCCTG GCACATACTT CAGTTGTGGA GGGTCAAAAA 
    GGTATTATTC CTCGTGCTAT CCAAGAAATA TTTCAAAACA TCTCTGGAAA GCCTAGCATT
    GACTTTAATA TAAAAGTATC TTATATAGAA GTATACAAGG AAGACCTAAG AGATCTTCTA
    GAATTGGAGA CATCAGTAAA AGATCTTCAC ATCCGAGAAG ATGAAAAAGG AAACACAGTG
    ATTGTTGGGG CCAAGGAATG CCAGGTGGAG AGTGCAGATG AAGTGATGAG TCTCCTGGAG
    ATGGGAAATG CAGCCAGGCA TACGGGTACG ACCCAAATGA ATGAGCACTC CAGTAGATCA
    CACGCGATTT TCACCATCAG CCTTTGTCAA GTGGACAAAA ATAGAAAGAC AGCTGAAGAT
    GGATCATGGC ATTCCCATCG GCATATTGTC TCAAAGTTCC ACTTTGTGGA TTTGGCTGGA
    TCAGAAAGAG TCACCAAAAC GGGGAATACC GGCGAACGTT TCAAAGAATC CATTCAGATC
    AACAGTGGGT TGCTGGCTTT AGGAAATGTA ATAAGCGCTC TTGGGGACCC ACGAAAGAAG
    AGTTCACATA TTCCATACAG GGATGCTAAA ATTACCCGGC TTCTGAAAGA CTCCCTGGGA
    GGCAGTGCCA AGACTGTCAT GATCACTTGT GTCAGCCCAT CCTCCTCAGA TTTTGACGAG
    TCCTTAAATT CCCTCAAATA TGCCAACAGA GCACGCAACA TTAGAAACAA ACCCACCTTA
    AACTTTAGCC CCGAGTCAGA CCGTATGGAC GAAATGGAAT TTGAGATTAA GTTGCTTCGA
    GAAGCTTTGC AAAGCCAGCA GGCTAGTAGC AGCCAAACTA GCCAGATCCA TCAGGAGGGG
    ACTCCTGATA AAAATAAGAT CCATTCTCTT GAGGAGCAAG TCACTCAGCT ACAAGGAGAA
    TGTCTGGGTT ACCAAAACTG TTTAGAGGAA GCCTTTACCT TCCTGGTGGA TTTAAAAGAT
    TCTGTGAGAC TAAATGAAAA GCAGCAACAC AAACTGCAGG AATGGTTTAA CATGACCCAG
    GAGGTCACCT TGTTCAGAGG ACACAGGGGC ATTGGAAACC TGGAAGAAGG ACCACAGCAT
    ATTACAGTTC TCCAGCTGAA GCGAGAGCTT AAGAAATGCC AGTGTGCTCT TGCTGCTGAT
    GAAGTAGTAT TTAATCAGAA GGACTTGGAG GTGAAGGAGC TAAAGAATCA AGTGCAAATG
    ATGGTGCAGG AAAACAAAGG ACATGTTGTT TCTTTGAAAG AAGCACAAAA AGTGAATAGA
    TTGCAGAATG AAAAAATAAT AGAACAACAA CTTCTTGTGG ATCAACTGAG TGAAGAACTA
    ACAAAACTTA ACCTGTCAAT GACTTCTTCA GTAAAGGAAA CTTGTGGAGA TGGGCCAGAT
    ACCAGGATAC TTGAAAAGAG ACCATACACT GTACCATTTG ATACTCGTTT GGGGCATTAT
    ATTTATGGTC CAGTAAGGTT GGATTCCAGG AAGGTCCACA CAAGTCCTTC TGTATACTCT
    CTGGATCGAC TAGTTGCGGG ATTTCGAACA CGAAGTCAGA TGCTTCTGGG TCGCTTAGAA
    GAACAGGATG AAGTTCTCCA GTGCCAGTTT TCTGATAACA GTGATGATGA AGAGTCAGAA
    GGCCAAGAGA AATCGGAAAT TAGACATAGA AGTTGCTCAT GGATTCAGAA GCCAGGCTCT
    GTTTGTTCAC TTGTTGAATT GAATGATATT CAGGATCAAA CACAAAAGTC AGGATTGGAG
    AATGAAGATT TAAAGATTGA ATGTCTCCAG GAGAGTCAAG AGTTAAATTT GCAAAAATTA
    AGAAATTCAG AACTCATACT TACTGAAGCT AAACAAAAAA TGAGAGAACT TACTATTAAC
    ATCAAGATGA AGGAAGATCT GATTAAAGAA TTAATTAAAA CAGGTAATGA TGCCAAGTCC
    GTAAGCAAGC AGTATTCTCT GAAAGTAACA AAACTTGAAC ATGAAGCAGA ACAGGCAAAA
    GTGGAATTAA CTGAAACAGA AAAGCAACTA CAGGAACTGG AAAACAAAGA TCTTTCTGAT
    GTTGCTTTGA AGGTAAAATT ACAGAAGGAA TTTCGTAGAA AGATGGATGC CGCTAAGCTA
    AGAGTCCAGG TCTTACAGAA GAAGCATCAA AATAGTAAGA AATTGGCATC ACTGTCAATC
    CAAAATGAGA AACGTGCTAA TGAACTAGAG CAGAATGTAG ATCACATGAA ATATCAAAAG
    GTACAGCTGC AAAAAAGACT TCGAGAAGAA AACGAAAAAA GGAAACAACT GGATGCAGAA
    ATTAAGCGGG ATCAACAAAA AATCAAAGAA CTACAGTTAA AAACAGAACA GAAAGATCTA
    AAACTGAAAG CTGAGGACCT TGATGCATTT AAGATGAAAA GGAGAAAAGG TTCGTTTGGA
    AGTATAGACC AACTCCAGAA ATTGGATAAG CAAAAGAAAT GGTTAGATGA AGAAGTAGAG
    AAAGTTCTGA ACCAACGCCA AGAATTAGAG GAGCTGGAAG AAGACTTAAA GAAACGGGAG
    GCCATAGTTT CAAAGAAGGA GGCCCTGTTA CAGGAGAAGA GCCACCTGGA AAGTAAGAAG
    TTGCGATCTA GTCAGGCTTT GAACACAGAC AGTTTAAAAA TATCAACTCG CCTGAGCTTG
    TTGGACCAAG AGTTGTCTGA AAAGAATGTG CAGCTCCAGA GCAGCACAGC TGAGGAGAAA
    ATAAAGATTT CAGAACAAGT ACAAGCCCTC CAGAGAGAAA AGGATCAGCT GCAGAGACAA
    AGAAACAGCG TGGATGAGAA ACTTAAAAAT GGTAGCGTGT TGTCACCTGA AGAAGAACAT
    GTTCTTTTCC AACTTGAAGA AGGGATTGAA GCTTTGGAAG CCGCAATTGA ATACAAGAAC
    GAAAGTATCC AGAGTCGCCA AAACTTGCTC AGAGCTTCAT TTCAAAACCT CTCTCATAGT
    GAAGCAAATG TCTTAGAAAA ACTAATTTGC CTGAGTCCTG TTGAGATTAG AGCTGTTCTT
    TTCAGATATT TCAATAAGGT GGTTAATTTG CGAGAAGTTG AACGGAAACT AAAGTTACAG
    AATGAAGAAA TTAAAATGAA AGTTCTGGAA CGGGATAACA TGGTTCGTGA GTTAGAATCT
    GCACTGGAAC ATCTGAAATT TCAGTGTGAC CGGAGACTGA TCCTCCAGCA AAAGGAACAT
    GAGCAAAAAA TGCAACTGCT GTTACATCAT TTCAAAGAGC GAGATGGGGA AGGCATTGTG
    GAAACTTTAA AAAATTATGA AGATAAAATC CATCAGCTGG AAAGAGATCT ATATTTCTAT
    AAGAAAACCA GCAGAGATCT GAAGAAGAAA CTCAAGGAAC TGGTAGGGGA AGCAATTCGG
    CGGCAACTAC TACCATCAGA AAATCATGAT GCTGGAGATG GAGTTCAGAA CCCAGAAAAG
    GCCGGCATGG TTTCAGAAGA ATTAAAATGG GCATCCAGAA CTGAAAGTAT GAAATTAAGT
    GGAAGGGAAA GACCATTAGA CAGTTCCACA AGCAGTTTAA GAACACAACC AAATCCTCAG
    AAGCTCTGGG AAGATGGCCC AGAATTAGCT CCAATGTATA GCTCTGTAGC ACCCACTAGT
    GGGCATTTGT TAAGCAATGA GGACAAAACC GAAATAGATG AAAATCAGTT TACAAAATCT
    AACAGTCGAC CATCATCCCA CATTCAGCCA GTCGGAAATG TGGGACAGCT GCATGGTGTC
    ACACCTGTAA AGTTGTGTCG CAAAGAGTTA CGCCAGATTT CTGCCTTGGA ACTATCATTA
    CGACGTTCCA ATCTTGGAGT TGCGGTCGGA TCTATGACTG CTGATTCCCT TGAAGTAGCT
    AGGAAAGTGA GTGACTTAAA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91341
    Clone ID Related Accession (Same CDS sequence) XM_015472622.1
    Accession Version XM_015472622.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104127.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGTG
    ATTGTTGGGG CCAAGGAATG CCAGGTGGAG AGTGCAGATG AAGTGATGAG TCTCCTGGAG
    ATGGGAAATG CAGCCAGGCA TACGGGTACG ACCCAAATGA ATGAGCACTC CAGTAGATCA
    CACGCGATTT TCACCATCAG CCTTTGTCAA GTGGACAAAA ATAGAAAGAC AGCTGAAGAT
    GGATCATGGC ATTCCCATCG GCATATTGTC TCAAAGTTCC ACTTTGTGGA TTTGGCTGGA
    TCAGAAAGAG TCACCAAAAC GGGGAATACC GGCGAACGTT TCAAAGAATC CATTCAGATC
    AACAGTGGGT TGCTGGCTTT AGGAAATGTA ATAAGCGCTC TTGGGGACCC ACGAAAGAAG
    AGTTCACATA TTCCATACAG GGATGCTAAA ATTACCCGGC TTCTGAAAGA CTCCCTGGGA
    GGCAGTGCCA AGACTGTCAT GATCACTTGT GTCAGCCCAT CCTCCTCAGA TTTTGACGAG
    TCCTTAAATT CCCTCAAATA TGCCAACAGA GCACGCAACA TTAGAAACAA ACCCACCTTA
    AACTTTAGCC CCGAGTCAGA CCGTATGGAC GAAATGGAAT TTGAGATTAA GTTGCTTCGA
    GAAGCTTTGC AAAGCCAGCA GGCTAGTAGC AGCCAAACTA GCCAGATCCA TCAGGAGGGG
    ACTCCTGATA AAAATAAGAT CCATTCTCTT GAGGAGCAAG TCACTCAGCT ACAAGGAGAA
    TGTCTGGGTT ACCAAAACTG TTTAGAGGAA GCCTTTACCT TCCTGGTGGA TTTAAAAGAT
    TCTGTGAGAC TAAATGAAAA GCAGCAACAC AAACTGCAGG AATGGTTTAA CATGACCCAG
    GAGGTCACCT TGTTCAGAGG ACACAGGGGC ATTGGAAACC TGGAAGAAGG ACCACAGCAT
    ATTACAGTTC TCCAGCTGAA GCGAGAGCTT AAGAAATGCC AGTGTGCTCT TGCTGCTGAT
    GAAGTAGTAT TTAATCAGAA GGACTTGGAG GTGAAGGAGC TAAAGAATCA AGTGCAAATG
    ATGGTGCAGG AAAACAAAGG ACATGTTGTT TCTTTGAAAG AAGCACAAAA AGTGAATAGA
    TTGCAGAATG AAAAAATAAT AGAACAACAA CTTCTTGTGG ATCAACTGAG TGAAGAACTA
    ACAAAACTTA ACCTGTCAAT GACTTCTTCA GTAAAGGAAA CTTGTGGAGA TGGGCCAGAT
    ACCAGGATAC TTGAAAAGAG ACCATACACT GTACCATTTG ATACTCGTTT GGGGCATTAT
    ATTTATGGTC CAGCAAGGTT GGATTCCAGG AAGGTCCACA CAAGTCCTTC TGTATACTCT
    CTGGATCGAC TAGTTGCGGG ATTTCGAACA CGAAGTCAGA TGCTTCTGGG TCGCTTAGAA
    GAACAGGATG AAGTTCTCCA GTGCCAGTTT TCTGATAACA GTGATGATGA AGAGTCAGAA
    GGCCAAGAGA AATCGGAAAT TAGACATAGA AGTTGCTCAT GGATTCAGAA GCCAGGCTCT
    GTTTGTTCAC TTGTTGAATT GAATGATATT CAGGATCAAA CACAAAAGTC AGGATTGGAG
    AATGAAGATT TAAAGATTGA ATGTCTCCAG GAGAGTCAAG AGTTAAATTT GCAAAAATTA
    AGAAATTCAG AACTCATACT TACTGAAGCT AAACAAAAAA TGAGAGAACT TACTATTAAC
    ATCAAGATGA AGGAAGATCT GATTAAAGAA TTAATTAAAA CAGGTAATGA TGCCAAGTCC
    GTAAGCAAGC AGTATTCTCT GAAAGTAACA AAACTTGAAC ATGAAGCAGA ACAGGCAAAA
    GTGGAATTAA CTGAAACAGA AAAGCAACTA CAGGAACTGG AAAACAAAGA TCTTTCTGAT
    GTTGCTTTGA AGGTAAAATT ACAGAAGGAA TTTCGTAGAA AGATGGATGC CGCTAAGCTA
    AGAGTCCAGG TCTTACAGAA GAAGCATCAA AATAGTAAGA AATTGGCATC ACTGTCAATC
    CAAAATGAGA AACGTGCTAA TGAACTAGAG CAGAATGTAG ATCACATGAA ATATCAAAAG
    GTACAGCTGC AAAAAAGACT TCGAGAAGAA AACGAAAAAA GGAAACAACT GGATGCAGAA
    ATTAAGCGGG ATCAACAAAA AATCAAAGAA CTACAGTTAA AAACAGAACA GAAAGATCTA
    AAACTGAAAG CTGAGGACCT TGATGCATTT AAGATGAAAA GGAGAAAAGG TTCGTTTGGA
    AGTATAGACC AACTCCAGAA ATTGGATAAG CAAAAGAAAT GGTTAGATGA AGAAGTAGAG
    AAAGTTCTGA ACCAACGCCA AGAATTAGAG GAGCTGGAAG AAGACTTAAA GAAACGGGAG
    GCCATAGTTT CAAAGAAGGA GGCCCTGTTA CAGGAGAAGA GCCACCTGGA AAGTAAGAAG
    TTGCGATCTA GTCAGGCTTT GAACACAGAC AGTTTAAAAA TATCAACTCG CCTGAGCTTG
    TTGGACCAAG AGTTGTCTGA AAAGAATGTG CAGCTCCAGA GCAGCACAGC TGAGGAGAAA
    ATAAAGATTT CAGAACAAGT ACAAGCCCTC CAGAGAGAAA AGGATCAGCT GCAGAGACAA
    AGAAACAGCG TGGATGAGAA ACTTAAAAAT GGTAGCGTGT TGTCACCTGA AGAAGAACAT
    GTTCTTTTCC AACTTGAAGA AGGGATTGAA GCTTTGGAAG CCGCAATTGA ATACAAGAAC
    GAAAGTATCC AGAGTCGCCA AAACTTGCTC AGAGCTTCAT TTCAAAACCT CTCTCATAGT
    GAAGCAAATG TCTTAGAAAA ACTAATTTGC CTGAGTCCTG TTGAGATTAG AGCTGTTCTT
    TTCAGATATT TCAATAAGGT GGTTAATTTG CGAGAAGTTG AACGGAAACT AAAGTTACAG
    AATGAAGAAA TTAAAATGAA AGTTCTGGAA CGGGATAACA TGGTTCGTGA GTTAGAATCT
    GCACTGGAAC ATCTGAAATT TCAGTGTGAC CGGAGACTGA TCCTCCAGCA AAAGGAACAT
    GAGCAAAAAA TGCAACTGCT GTTACATCAT TTCAAAGAGC GAGATGGGGA AGGCATTGTG
    GAAACTTTAA AAAATTATGA AGATAAAATC CATCAGCTGG AAAGAGATCT ATATTTCTAT
    AAGAAAACCA GCAGAGATCT GAAGAAGAAA CTCAAGGAAC TGGTAGGGGA AGCAATTCGG
    CGGCAACTAC TACCATCAGA AAATCATGAT GCTGGAGATG GAGTTCAGAA CCCAGAAAAG
    GCCGGCATGG TTTCAGAAGA ATTAAAATGG GCATCCAGAA CTGAAAGTAT GAAATTAAGT
    GGAAGGGAAA GACCATTAGA CAGTTCCACA AGCAGTTTAA GAACACAACC AAATCCTCAG
    AAGCTCTGGG AAGATGGCCC AGAATTAGCT CCAATGTATA GCTCTGTAGC ACCCACTAGT
    GGGCATTTGT TAAGCAATGA GGACAAAACC GAAATAGATG AAAATCAGTT TACAAAATCT
    AACAGTCGAC CATCATCCCA CATTCAGCCA GTCGGAAATG TGGGACAGCT GCATGGTGTC
    ACACCTGTAA AGTTGTGTCG CAAAGAGTTA CGCCAGATTT CTGCCTTGGA ACTATCATTA
    CGACGTTCCA ATCTTGGAGT TGCGGTCGGA TCTATGACTG CTGATTCCCT TGAAGTAGCT
    AGGAAAGTGA GTGACTTAAA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328108.1
    CDS237..4223
    Translation

    Target ORF information:

    RefSeq Version XM_015472622.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472622.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGTG
    ATTGTTGGGG CCAAGGAATG CCAGGTGGAG AGTGCAGATG AAGTGATGAG TCTCCTGGAG
    ATGGGAAATG CAGCCAGGCA TACGGGTACG ACCCAAATGA ATGAGCACTC CAGTAGATCA
    CACGCGATTT TCACCATCAG CCTTTGTCAA GTGGACAAAA ATAGAAAGAC AGCTGAAGAT
    GGATCATGGC ATTCCCATCG GCATATTGTC TCAAAGTTCC ACTTTGTGGA TTTGGCTGGA
    TCAGAAAGAG TCACCAAAAC GGGGAATACC GGCGAACGTT TCAAAGAATC CATTCAGATC
    AACAGTGGGT TGCTGGCTTT AGGAAATGTA ATAAGCGCTC TTGGGGACCC ACGAAAGAAG
    AGTTCACATA TTCCATACAG GGATGCTAAA ATTACCCGGC TTCTGAAAGA CTCCCTGGGA
    GGCAGTGCCA AGACTGTCAT GATCACTTGT GTCAGCCCAT CCTCCTCAGA TTTTGACGAG
    TCCTTAAATT CCCTCAAATA TGCCAACAGA GCACGCAACA TTAGAAACAA ACCCACCTTA
    AACTTTAGCC CCGAGTCAGA CCGTATGGAC GAAATGGAAT TTGAGATTAA GTTGCTTCGA
    GAAGCTTTGC AAAGCCAGCA GGCTAGTAGC AGCCAAACTA GCCAGATCCA TCAGGAGGGG
    ACTCCTGATA AAAATAAGAT CCATTCTCTT GAGGAGCAAG TCACTCAGCT ACAAGGAGAA
    TGTCTGGGTT ACCAAAACTG TTTAGAGGAA GCCTTTACCT TCCTGGTGGA TTTAAAAGAT
    TCTGTGAGAC TAAATGAAAA GCAGCAACAC AAACTGCAGG AATGGTTTAA CATGACCCAG
    GAGGTCACCT TGTTCAGAGG ACACAGGGGC ATTGGAAACC TGGAAGAAGG ACCACAGCAT
    ATTACAGTTC TCCAGCTGAA GCGAGAGCTT AAGAAATGCC AGTGTGCTCT TGCTGCTGAT
    GAAGTAGTAT TTAATCAGAA GGACTTGGAG GTGAAGGAGC TAAAGAATCA AGTGCAAATG
    ATGGTGCAGG AAAACAAAGG ACATGTTGTT TCTTTGAAAG AAGCACAAAA AGTGAATAGA
    TTGCAGAATG AAAAAATAAT AGAACAACAA CTTCTTGTGG ATCAACTGAG TGAAGAACTA
    ACAAAACTTA ACCTGTCAAT GACTTCTTCA GTAAAGGAAA CTTGTGGAGA TGGGCCAGAT
    ACCAGGATAC TTGAAAAGAG ACCATACACT GTACCATTTG ATACTCGTTT GGGGCATTAT
    ATTTATGGTC CAGCAAGGTT GGATTCCAGG AAGGTCCACA CAAGTCCTTC TGTATACTCT
    CTGGATCGAC TAGTTGCGGG ATTTCGAACA CGAAGTCAGA TGCTTCTGGG TCGCTTAGAA
    GAACAGGATG AAGTTCTCCA GTGCCAGTTT TCTGATAACA GTGATGATGA AGAGTCAGAA
    GGCCAAGAGA AATCGGAAAT TAGACATAGA AGTTGCTCAT GGATTCAGAA GCCAGGCTCT
    GTTTGTTCAC TTGTTGAATT GAATGATATT CAGGATCAAA CACAAAAGTC AGGATTGGAG
    AATGAAGATT TAAAGATTGA ATGTCTCCAG GAGAGTCAAG AGTTAAATTT GCAAAAATTA
    AGAAATTCAG AACTCATACT TACTGAAGCT AAACAAAAAA TGAGAGAACT TACTATTAAC
    ATCAAGATGA AGGAAGATCT GATTAAAGAA TTAATTAAAA CAGGTAATGA TGCCAAGTCC
    GTAAGCAAGC AGTATTCTCT GAAAGTAACA AAACTTGAAC ATGAAGCAGA ACAGGCAAAA
    GTGGAATTAA CTGAAACAGA AAAGCAACTA CAGGAACTGG AAAACAAAGA TCTTTCTGAT
    GTTGCTTTGA AGGTAAAATT ACAGAAGGAA TTTCGTAGAA AGATGGATGC CGCTAAGCTA
    AGAGTCCAGG TCTTACAGAA GAAGCATCAA AATAGTAAGA AATTGGCATC ACTGTCAATC
    CAAAATGAGA AACGTGCTAA TGAACTAGAG CAGAATGTAG ATCACATGAA ATATCAAAAG
    GTACAGCTGC AAAAAAGACT TCGAGAAGAA AACGAAAAAA GGAAACAACT GGATGCAGAA
    ATTAAGCGGG ATCAACAAAA AATCAAAGAA CTACAGTTAA AAACAGAACA GAAAGATCTA
    AAACTGAAAG CTGAGGACCT TGATGCATTT AAGATGAAAA GGAGAAAAGG TTCGTTTGGA
    AGTATAGACC AACTCCAGAA ATTGGATAAG CAAAAGAAAT GGTTAGATGA AGAAGTAGAG
    AAAGTTCTGA ACCAACGCCA AGAATTAGAG GAGCTGGAAG AAGACTTAAA GAAACGGGAG
    GCCATAGTTT CAAAGAAGGA GGCCCTGTTA CAGGAGAAGA GCCACCTGGA AAGTAAGAAG
    TTGCGATCTA GTCAGGCTTT GAACACAGAC AGTTTAAAAA TATCAACTCG CCTGAGCTTG
    TTGGACCAAG AGTTGTCTGA AAAGAATGTG CAGCTCCAGA GCAGCACAGC TGAGGAGAAA
    ATAAAGATTT CAGAACAAGT ACAAGCCCTC CAGAGAGAAA AGGATCAGCT GCAGAGACAA
    AGAAACAGCG TGGATGAGAA ACTTAAAAAT GGTAGCGTGT TGTCACCTGA AGAAGAACAT
    GTTCTTTTCC AACTTGAAGA AGGGATTGAA GCTTTGGAAG CCGCAATTGA ATACAAGAAC
    GAAAGTATCC AGAGTCGCCA AAACTTGCTC AGAGCTTCAT TTCAAAACCT CTCTCATAGT
    GAAGCAAATG TCTTAGAAAA ACTAATTTGC CTGAGTCCTG TTGAGATTAG AGCTGTTCTT
    TTCAGATATT TCAATAAGGT GGTTAATTTG CGAGAAGTTG AACGGAAACT AAAGTTACAG
    AATGAAGAAA TTAAAATGAA AGTTCTGGAA CGGGATAACA TGGTTCGTGA GTTAGAATCT
    GCACTGGAAC ATCTGAAATT TCAGTGTGAC CGGAGACTGA TCCTCCAGCA AAAGGAACAT
    GAGCAAAAAA TGCAACTGCT GTTACATCAT TTCAAAGAGC GAGATGGGGA AGGCATTGTG
    GAAACTTTAA AAAATTATGA AGATAAAATC CATCAGCTGG AAAGAGATCT ATATTTCTAT
    AAGAAAACCA GCAGAGATCT GAAGAAGAAA CTCAAGGAAC TGGTAGGGGA AGCAATTCGG
    CGGCAACTAC TACCATCAGA AAATCATGAT GCTGGAGATG GAGTTCAGAA CCCAGAAAAG
    GCCGGCATGG TTTCAGAAGA ATTAAAATGG GCATCCAGAA CTGAAAGTAT GAAATTAAGT
    GGAAGGGAAA GACCATTAGA CAGTTCCACA AGCAGTTTAA GAACACAACC AAATCCTCAG
    AAGCTCTGGG AAGATGGCCC AGAATTAGCT CCAATGTATA GCTCTGTAGC ACCCACTAGT
    GGGCATTTGT TAAGCAATGA GGACAAAACC GAAATAGATG AAAATCAGTT TACAAAATCT
    AACAGTCGAC CATCATCCCA CATTCAGCCA GTCGGAAATG TGGGACAGCT GCATGGTGTC
    ACACCTGTAA AGTTGTGTCG CAAAGAGTTA CGCCAGATTT CTGCCTTGGA ACTATCATTA
    CGACGTTCCA ATCTTGGAGT TGCGGTCGGA TCTATGACTG CTGATTCCCT TGAAGTAGCT
    AGGAAAGTGA GTGACTTAAA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91342
    Clone ID Related Accession (Same CDS sequence) XM_015472623.1
    Accession Version XM_015472623.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104127.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGTTCCTG GGGTCCTAGA ATACTGCCTG GCACATACTT CAGTTGTGGA GGGTCAAAAA 
    GGTATTATTC CTCGTGCTAT CCAAGAAATA TTTCAAAACA TCTCTGGAAA GCCTAGCATT
    GACTTTAATA TAAAAGTATC TTATATAGAA GTATACAAGG AAGACCTAAG AGATCTTCTA
    GAATTGGAGA CATCAGTAAA AGATCTTCAC ATCCGAGAAG ATGAAAAAGG AAACACAGTG
    ATTGTTGGGG CCAAGGAATG CCAGGTGGAG AGTGCAGATG AAGTGATGAG TCTCCTGGAG
    ATGGGAAATG CAGCCAGGCA TACGGGTACG ACCCAAATGA ATGAGCACTC CAGTAGATCA
    CACGCGATTT TCACCATCAG CCTTTGTCAA GTGGACAAAA ATAGAAAGAC AGCTGAAGAT
    GGATCATGGC ATTCCCATCG GCATATTGTC TCAAAGTTCC ACTTTGTGGA TTTGGCTGGA
    TCAGAAAGAG TCACCAAAAC GGGGAATACC GGCGAACGTT TCAAAGAATC CATTCAGATC
    AACAGTGGGT TGCTGGCTTT AGGAAATGTA ATAAGCGCTC TTGGGGACCC ACGAAAGAAG
    AGTTCACATA TTCCATACAG GGATGCTAAA ATTACCCGGC TTCTGAAAGA CTCCCTGGGA
    GGCAGTGCCA AGACTGTCAT GATCACTTGT GTCAGCCCAT CCTCCTCAGA TTTTGACGAG
    TCCTTAAATT CCCTCAAATA TGCCAACAGA GCACGCAACA TTAGAAACAA ACCCACCTTA
    AACTTTAGCC CCGAGTCAGA CCGTATGGAC GAAATGGAAT TTGAGATTAA GTTGCTTCGA
    GAAGCTTTGC AAAGCCAGCA GGCTAGTAGC AGCCAAACTA GCCAGATCCA TCAGGAGGGG
    ACTCCTGATA AAAATAAGAT CCATTCTCTT GAGGAGCAAG TCACTCAGCT ACAAGGAGAA
    TGTCTGGGTT ACCAAAACTG TTTAGAGGAA GCCTTTACCT TCCTGGTGGA TTTAAAAGAT
    TCTGTGAGAC TAAATGAAAA GCAGCAACAC AAACTGCAGG AATGGTTTAA CATGACCCAG
    GAGGTCACCT TGTTCAGAGG ACACAGGGGC ATTGGAAACC TGGAAGAAGG ACCACAGCAT
    ATTACAGTTC TCCAGCTGAA GCGAGAGCTT AAGAAATGCC AGTGTGCTCT TGCTGCTGAT
    GAAGTAGTAT TTAATCAGAA GGACTTGGAG GTGAAGGAGC TAAAGAATCA AGTGCAAATG
    ATGGTGCAGG AAAACAAAGG ACATGTTGTT TCTTTGAAAG AAGCACAAAA AGTGAATAGA
    TTGCAGAATG AAAAAATAAT AGAACAACAA CTTCTTGTGG ATCAACTGAG TGAAGAACTA
    ACAAAACTTA ACCTGTCAAT GACTTCTTCA GTAAAGGAAA CTTGTGGAGA TGGGCCAGAT
    ACCAGGATAC TTGAAAAGAG ACCATACACT GTACCATTTG ATACTCGTTT GGGGCATTAT
    ATTTATGGTC CAGCAAGGTT GGATTCCAGG AAGGTCCACA CAAGTCCTTC TGTATACTCT
    CTGGATCGAC TAGTTGCGGG ATTTCGAACA CGAAGTCAGA TGCTTCTGGG TCGCTTAGAA
    GAACAGGATG AAGTTCTCCA GTGCCAGTTT TCTGATAACA GTGATGATGA AGAGTCAGAA
    GGCCAAGAGA AATCGGAAAT TAGACATAGA AGTTGCTCAT GGATTCAGAA GCCAGGCTCT
    GTTTGTTCAC TTGTTGAATT GAATGATATT CAGGATCAAA CACAAAAGTC AGGATTGGAG
    AATGAAGATT TAAAGATTGA ATGTCTCCAG GAGAGTCAAG AGTTAAATTT GCAAAAATTA
    AGAAATTCAG AACTCATACT TACTGAAGCT AAACAAAAAA TGAGAGAACT TACTATTAAC
    ATCAAGATGA AGGAAGATCT GATTAAAGAA TTAATTAAAA CAGGTAATGA TGCCAAGTCC
    GTAAGCAAGC AGTATTCTCT GAAAGTAACA AAACTTGAAC ATGAAGCAGA ACAGGCAAAA
    GTGGAATTAA CTGAAACAGA AAAGCAACTA CAGGAACTGG AAAACAAAGA TCTTTCTGAT
    GTTGCTTTGA AGGTAAAATT ACAGAAGGAA TTTCGTAGAA AGATGGATGC CGCTAAGCTA
    AGAGTCCAGG TCTTACAGAA GAAGCATCAA AATAGTAAGA AATTGGCATC ACTGTCAATC
    CAAAATGAGA AACGTGCTAA TGAACTAGAG CAGAATGTAG ATCACATGAA ATATCAAAAG
    GTACAGCTGC AAAAAAGACT TCGAGAAGAA AACGAAAAAA GGAAACAACT GGATGCAGAA
    ATTAAGCGGG ATCAACAAAA AATCAAAGAA CTACAGTTAA AAACAGAACA GAAAGATCTA
    AAACTGAAAG CTGAGGACCT TGATGCATTT AAGATGAAAA GGAGAAAAGG TTCGTTTGGA
    AGTATAGACC AACTCCAGAA ATTGGATAAG CAAAAGAAAT GGTTAGATGA AGAAGTAGAG
    AAAGTTCTGA ACCAACGCCA AGAATTAGAG GAGCTGGAAG AAGACTTAAA GAAACGGGAG
    GCCATAGTTT CAAAGAAGGA GGCCCTGTTA CAGGAGAAGA GCCACCTGGA AAGTAAGAAG
    TTGCGATCTA GTCAGGCTTT GAACACAGAC AGTTTAAAAA TATCAACTCG CCTGAGCTTG
    TTGGACCAAG AGTTGTCTGA AAAGAATGTG CAGCTCCAGA GCAGCACAGC TGAGGAGAAA
    ATAAAGATTT CAGAACAAGT ACAAGCCCTC CAGAGAGAAA AGGATCAGCT GCAGAGACAA
    AGAAACAGCG TGGATGAGAA ACTTAAAAAT GGTAGCGTGT TGTCACCTGA AGAAGAACAT
    GTTCTTTTCC AACTTGAAGA AGGGATTGAA GCTTTGGAAG CCGCAATTGA ATACAAGAAC
    GAAAGTATCC AGAGTCGCCA AAACTTGCTC AGAGCTTCAT TTCAAAACCT CTCTCATAGT
    GAAGCAAATG TCTTAGAAAA ACTAATTTGC CTGAGTCCTG TTGAGATTAG AGCTGTTCTT
    TTCAGATATT TCAATAAGGT GGTTAATTTG CGAGAAGTTG AACGGAAACT AAAGTTACAG
    AATGAAGAAA TTAAAATGAA AGTTCTGGAA CGGGATAACA TGGTTCGTGA GTTAGAATCT
    GCACTGGAAC ATCTGAAATT TCAGTGTGAC CGGAGACTGA TCCTCCAGCA AAAGGAACAT
    GAGCAAAAAA TGCAACTGCT GTTACATCAT TTCAAAGAGC GAGATGGGGA AGGCATTGTG
    GAAACTTTAA AAAATTATGA AGATAAAATC CATCAGCTGG AAAGAGATCT ATATTTCTAT
    AAGAAAACCA GCAGAGATCT GAAGAAGAAA CTCAAGGAAC TGGTAGGGGA AGCAATTCGG
    CGGCAACTAC TACCATCAGA AAATCATGAT GCTGGAGATG GAGTTCAGAA CCCAGAAAAG
    GCCGGCATGG TTTCAGAAGA ATTAAAATGG GCATCCAGAA CTGAAAGTAT GAAATTAAGT
    GGAAGGGAAA GACCATTAGA CAGTTCCACA AGCAGTTTAA GAACACAACC AAATCCTCAG
    AAGCTCTGGG AAGATGGCCC AGAATTAGCT CCAATGTATA GCTCTGTAGC ACCCACTAGT
    GGGCATTTGT TAAGCAATGA GGACAAAACC GAAATAGATG AAAATCAGTT TACAAAATCT
    AACAGTCGAC CATCATCCCA CATTCAGCCA GTCGGAAATG TGGGACAGCT GCATGGTGTC
    ACACCTGTAA AGTTGTGTCG CAAAGAGTTA CGCCAGATTT CTGCCTTGGA ACTATCATTA
    CGACGTTCCA ATCTTGGAGT TGCGGTCGGA TCTATGACTG CTGATTCCCT TGAAGTAGCT
    AGGAAAGTGA GTGACTTAAA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328109.1
    CDS1793..5719
    Translation

    Target ORF information:

    RefSeq Version XM_015472623.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472623.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGTTCCTG GGGTCCTAGA ATACTGCCTG GCACATACTT CAGTTGTGGA GGGTCAAAAA 
    GGTATTATTC CTCGTGCTAT CCAAGAAATA TTTCAAAACA TCTCTGGAAA GCCTAGCATT
    GACTTTAATA TAAAAGTATC TTATATAGAA GTATACAAGG AAGACCTAAG AGATCTTCTA
    GAATTGGAGA CATCAGTAAA AGATCTTCAC ATCCGAGAAG ATGAAAAAGG AAACACAGTG
    ATTGTTGGGG CCAAGGAATG CCAGGTGGAG AGTGCAGATG AAGTGATGAG TCTCCTGGAG
    ATGGGAAATG CAGCCAGGCA TACGGGTACG ACCCAAATGA ATGAGCACTC CAGTAGATCA
    CACGCGATTT TCACCATCAG CCTTTGTCAA GTGGACAAAA ATAGAAAGAC AGCTGAAGAT
    GGATCATGGC ATTCCCATCG GCATATTGTC TCAAAGTTCC ACTTTGTGGA TTTGGCTGGA
    TCAGAAAGAG TCACCAAAAC GGGGAATACC GGCGAACGTT TCAAAGAATC CATTCAGATC
    AACAGTGGGT TGCTGGCTTT AGGAAATGTA ATAAGCGCTC TTGGGGACCC ACGAAAGAAG
    AGTTCACATA TTCCATACAG GGATGCTAAA ATTACCCGGC TTCTGAAAGA CTCCCTGGGA
    GGCAGTGCCA AGACTGTCAT GATCACTTGT GTCAGCCCAT CCTCCTCAGA TTTTGACGAG
    TCCTTAAATT CCCTCAAATA TGCCAACAGA GCACGCAACA TTAGAAACAA ACCCACCTTA
    AACTTTAGCC CCGAGTCAGA CCGTATGGAC GAAATGGAAT TTGAGATTAA GTTGCTTCGA
    GAAGCTTTGC AAAGCCAGCA GGCTAGTAGC AGCCAAACTA GCCAGATCCA TCAGGAGGGG
    ACTCCTGATA AAAATAAGAT CCATTCTCTT GAGGAGCAAG TCACTCAGCT ACAAGGAGAA
    TGTCTGGGTT ACCAAAACTG TTTAGAGGAA GCCTTTACCT TCCTGGTGGA TTTAAAAGAT
    TCTGTGAGAC TAAATGAAAA GCAGCAACAC AAACTGCAGG AATGGTTTAA CATGACCCAG
    GAGGTCACCT TGTTCAGAGG ACACAGGGGC ATTGGAAACC TGGAAGAAGG ACCACAGCAT
    ATTACAGTTC TCCAGCTGAA GCGAGAGCTT AAGAAATGCC AGTGTGCTCT TGCTGCTGAT
    GAAGTAGTAT TTAATCAGAA GGACTTGGAG GTGAAGGAGC TAAAGAATCA AGTGCAAATG
    ATGGTGCAGG AAAACAAAGG ACATGTTGTT TCTTTGAAAG AAGCACAAAA AGTGAATAGA
    TTGCAGAATG AAAAAATAAT AGAACAACAA CTTCTTGTGG ATCAACTGAG TGAAGAACTA
    ACAAAACTTA ACCTGTCAAT GACTTCTTCA GTAAAGGAAA CTTGTGGAGA TGGGCCAGAT
    ACCAGGATAC TTGAAAAGAG ACCATACACT GTACCATTTG ATACTCGTTT GGGGCATTAT
    ATTTATGGTC CAGCAAGGTT GGATTCCAGG AAGGTCCACA CAAGTCCTTC TGTATACTCT
    CTGGATCGAC TAGTTGCGGG ATTTCGAACA CGAAGTCAGA TGCTTCTGGG TCGCTTAGAA
    GAACAGGATG AAGTTCTCCA GTGCCAGTTT TCTGATAACA GTGATGATGA AGAGTCAGAA
    GGCCAAGAGA AATCGGAAAT TAGACATAGA AGTTGCTCAT GGATTCAGAA GCCAGGCTCT
    GTTTGTTCAC TTGTTGAATT GAATGATATT CAGGATCAAA CACAAAAGTC AGGATTGGAG
    AATGAAGATT TAAAGATTGA ATGTCTCCAG GAGAGTCAAG AGTTAAATTT GCAAAAATTA
    AGAAATTCAG AACTCATACT TACTGAAGCT AAACAAAAAA TGAGAGAACT TACTATTAAC
    ATCAAGATGA AGGAAGATCT GATTAAAGAA TTAATTAAAA CAGGTAATGA TGCCAAGTCC
    GTAAGCAAGC AGTATTCTCT GAAAGTAACA AAACTTGAAC ATGAAGCAGA ACAGGCAAAA
    GTGGAATTAA CTGAAACAGA AAAGCAACTA CAGGAACTGG AAAACAAAGA TCTTTCTGAT
    GTTGCTTTGA AGGTAAAATT ACAGAAGGAA TTTCGTAGAA AGATGGATGC CGCTAAGCTA
    AGAGTCCAGG TCTTACAGAA GAAGCATCAA AATAGTAAGA AATTGGCATC ACTGTCAATC
    CAAAATGAGA AACGTGCTAA TGAACTAGAG CAGAATGTAG ATCACATGAA ATATCAAAAG
    GTACAGCTGC AAAAAAGACT TCGAGAAGAA AACGAAAAAA GGAAACAACT GGATGCAGAA
    ATTAAGCGGG ATCAACAAAA AATCAAAGAA CTACAGTTAA AAACAGAACA GAAAGATCTA
    AAACTGAAAG CTGAGGACCT TGATGCATTT AAGATGAAAA GGAGAAAAGG TTCGTTTGGA
    AGTATAGACC AACTCCAGAA ATTGGATAAG CAAAAGAAAT GGTTAGATGA AGAAGTAGAG
    AAAGTTCTGA ACCAACGCCA AGAATTAGAG GAGCTGGAAG AAGACTTAAA GAAACGGGAG
    GCCATAGTTT CAAAGAAGGA GGCCCTGTTA CAGGAGAAGA GCCACCTGGA AAGTAAGAAG
    TTGCGATCTA GTCAGGCTTT GAACACAGAC AGTTTAAAAA TATCAACTCG CCTGAGCTTG
    TTGGACCAAG AGTTGTCTGA AAAGAATGTG CAGCTCCAGA GCAGCACAGC TGAGGAGAAA
    ATAAAGATTT CAGAACAAGT ACAAGCCCTC CAGAGAGAAA AGGATCAGCT GCAGAGACAA
    AGAAACAGCG TGGATGAGAA ACTTAAAAAT GGTAGCGTGT TGTCACCTGA AGAAGAACAT
    GTTCTTTTCC AACTTGAAGA AGGGATTGAA GCTTTGGAAG CCGCAATTGA ATACAAGAAC
    GAAAGTATCC AGAGTCGCCA AAACTTGCTC AGAGCTTCAT TTCAAAACCT CTCTCATAGT
    GAAGCAAATG TCTTAGAAAA ACTAATTTGC CTGAGTCCTG TTGAGATTAG AGCTGTTCTT
    TTCAGATATT TCAATAAGGT GGTTAATTTG CGAGAAGTTG AACGGAAACT AAAGTTACAG
    AATGAAGAAA TTAAAATGAA AGTTCTGGAA CGGGATAACA TGGTTCGTGA GTTAGAATCT
    GCACTGGAAC ATCTGAAATT TCAGTGTGAC CGGAGACTGA TCCTCCAGCA AAAGGAACAT
    GAGCAAAAAA TGCAACTGCT GTTACATCAT TTCAAAGAGC GAGATGGGGA AGGCATTGTG
    GAAACTTTAA AAAATTATGA AGATAAAATC CATCAGCTGG AAAGAGATCT ATATTTCTAT
    AAGAAAACCA GCAGAGATCT GAAGAAGAAA CTCAAGGAAC TGGTAGGGGA AGCAATTCGG
    CGGCAACTAC TACCATCAGA AAATCATGAT GCTGGAGATG GAGTTCAGAA CCCAGAAAAG
    GCCGGCATGG TTTCAGAAGA ATTAAAATGG GCATCCAGAA CTGAAAGTAT GAAATTAAGT
    GGAAGGGAAA GACCATTAGA CAGTTCCACA AGCAGTTTAA GAACACAACC AAATCCTCAG
    AAGCTCTGGG AAGATGGCCC AGAATTAGCT CCAATGTATA GCTCTGTAGC ACCCACTAGT
    GGGCATTTGT TAAGCAATGA GGACAAAACC GAAATAGATG AAAATCAGTT TACAAAATCT
    AACAGTCGAC CATCATCCCA CATTCAGCCA GTCGGAAATG TGGGACAGCT GCATGGTGTC
    ACACCTGTAA AGTTGTGTCG CAAAGAGTTA CGCCAGATTT CTGCCTTGGA ACTATCATTA
    CGACGTTCCA ATCTTGGAGT TGCGGTCGGA TCTATGACTG CTGATTCCCT TGAAGTAGCT
    AGGAAAGTGA GTGACTTAAA AACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22226
    Clone ID Related Accession (Same CDS sequence) XM_015472619.1 , XM_015472620.1 , XM_015472621.1 , XM_005210336.3 , NM_001206123.2
    Accession Version NM_001206123.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02023901.1. On Jun 30, 2017 this sequence version replaced NM_001206123.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN04019635 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193052.1
    CDS1..4188
    Translation

    Target ORF information:

    RefSeq Version NM_001206123.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206123.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa41053
    Clone ID Related Accession (Same CDS sequence) NM_001206123.1
    Accession Version NM_001206123.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product kinesin-like protein KIF27
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03019285.1, AAFC03019283.1, AAFC03045066.1, AAFC03019284.1, AAFC03045068.1 and AAFC03128212.1. On Apr 20, 2011 this sequence version replaced XM_863957.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTTGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193052.1
    CDS1..4188
    Translation

    Target ORF information:

    RefSeq Version NM_001206123.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206123.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTTGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22226
    Clone ID Related Accession (Same CDS sequence) XM_015472619.1 , XM_015472620.1 , XM_015472621.1 , XM_005210336.3 , NM_001206123.2
    Accession Version XM_015472619.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104127.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328105.1
    CDS392..4579
    Translation

    Target ORF information:

    RefSeq Version XM_015472619.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472619.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22226
    Clone ID Related Accession (Same CDS sequence) XM_015472619.1 , XM_015472620.1 , XM_015472621.1 , XM_005210336.3 , NM_001206123.2
    Accession Version XM_015472620.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104127.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328106.1
    CDS396..4583
    Translation

    Target ORF information:

    RefSeq Version XM_015472620.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472620.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22226
    Clone ID Related Accession (Same CDS sequence) XM_015472619.1 , XM_015472620.1 , XM_015472621.1 , XM_005210336.3 , NM_001206123.2
    Accession Version XM_015472621.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104127.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328107.1
    CDS241..4428
    Translation

    Target ORF information:

    RefSeq Version XM_015472621.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472621.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22226
    Clone ID Related Accession (Same CDS sequence) XM_015472619.1 , XM_015472620.1 , XM_015472621.1 , XM_005210336.3 , NM_001206123.2
    Accession Version XM_005210336.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinesin-like protein KIF27 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104127.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210336.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210393.1
    CDS237..4424
    Translation

    Target ORF information:

    RefSeq Version XM_005210336.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinesin family member 27 (KIF27), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210336.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAA TACCAGTTAA AGTTGCTATA AGAATTAGAC CTCTGCTGTG CAAAGAAGTT 
    CTTCATAATC ATCAAGCTTG TGTGAGAGTT ATTCCAAACA CCCAACAAAT TATCATTGGG
    AGAGATAGAA TCTTTACTTT TGATTTTGTT TTTGGCAAAA ATTCCACTCA AGATGAAGTT
    TATAATACCT GTATAAAGCC ATTAGTGTTG TCACTCATTG AGGGCTATAA TGCAACTGTT
    TTTGCCTATG GACAGACTGG ATCTGGGAAG ACATACACCA TCGGAGGAGG CCATGTTGCT
    TCAGTTGTGG AGGGTCAAAA AGGTATTATT CCTCGTGCTA TCCAAGAAAT ATTTCAAAAC
    ATCTCTGGAA AGCCTAGCAT TGACTTTAAT ATAAAAGTAT CTTATATAGA AGTATACAAG
    GAAGACCTAA GAGATCTTCT AGAATTGGAG ACATCAGTAA AAGATCTTCA CATCCGAGAA
    GATGAAAAAG GAAACACAGT GATTGTTGGG GCCAAGGAAT GCCAGGTGGA GAGTGCAGAT
    GAAGTGATGA GTCTCCTGGA GATGGGAAAT GCAGCCAGGC ATACGGGTAC GACCCAAATG
    AATGAGCACT CCAGTAGATC ACACGCGATT TTCACCATCA GCCTTTGTCA AGTGGACAAA
    AATAGAAAGA CAGCTGAAGA TGGATCATGG CATTCCCATC GGCATATTGT CTCAAAGTTC
    CACTTTGTGG ATTTGGCTGG ATCAGAAAGA GTCACCAAAA CGGGGAATAC CGGCGAACGT
    TTCAAAGAAT CCATTCAGAT CAACAGTGGG TTGCTGGCTT TAGGAAATGT AATAAGCGCT
    CTTGGGGACC CACGAAAGAA GAGTTCACAT ATTCCATACA GGGATGCTAA AATTACCCGG
    CTTCTGAAAG ACTCCCTGGG AGGCAGTGCC AAGACTGTCA TGATCACTTG TGTCAGCCCA
    TCCTCCTCAG ATTTTGACGA GTCCTTAAAT TCCCTCAAAT ATGCCAACAG AGCACGCAAC
    ATTAGAAACA AACCCACCTT AAACTTTAGC CCCGAGTCAG ACCGTATGGA CGAAATGGAA
    TTTGAGATTA AGTTGCTTCG AGAAGCTTTG CAAAGCCAGC AGGCTAGTAG CAGCCAAACT
    AGCCAGATCC ATCAGGAGGG GACTCCTGAT AAAAATAAGA TCCATTCTCT TGAGGAGCAA
    GTCACTCAGC TACAAGGAGA ATGTCTGGGT TACCAAAACT GTTTAGAGGA AGCCTTTACC
    TTCCTGGTGG ATTTAAAAGA TTCTGTGAGA CTAAATGAAA AGCAGCAACA CAAACTGCAG
    GAATGGTTTA ACATGACCCA GGAGGTCACC TTGTTCAGAG GACACAGGGG CATTGGAAAC
    CTGGAAGAAG GACCACAGCA TATTACAGTT CTCCAGCTGA AGCGAGAGCT TAAGAAATGC
    CAGTGTGCTC TTGCTGCTGA TGAAGTAGTA TTTAATCAGA AGGACTTGGA GGTGAAGGAG
    CTAAAGAATC AAGTGCAAAT GATGGTGCAG GAAAACAAAG GACATGTTGT TTCTTTGAAA
    GAAGCACAAA AAGTGAATAG ATTGCAGAAT GAAAAAATAA TAGAACAACA ACTTCTTGTG
    GATCAACTGA GTGAAGAACT AACAAAACTT AACCTGTCAA TGACTTCTTC AGTAAAGGAA
    ACTTGTGGAG ATGGGCCAGA TACCAGGATA CTTGAAAAGA GACCATACAC TGTACCATTT
    GATACTCGTT TGGGGCATTA TATTTATGGT CCAGCAAGGT TGGATTCCAG GAAGGTCCAC
    ACAAGTCCTT CTGTATACTC TCTGGATCGA CTAGTTGCGG GATTTCGAAC ACGAAGTCAG
    ATGCTTCTGG GTCGCTTAGA AGAACAGGAT GAAGTTCTCC AGTGCCAGTT TTCTGATAAC
    AGTGATGATG AAGAGTCAGA AGGCCAAGAG AAATCGGAAA TTAGACATAG AAGTTGCTCA
    TGGATTCAGA AGCCAGGCTC TGTTTGTTCA CTTGTTGAAT TGAATGATAT TCAGGATCAA
    ACACAAAAGT CAGGATTGGA GAATGAAGAT TTAAAGATTG AATGTCTCCA GGAGAGTCAA
    GAGTTAAATT TGCAAAAATT AAGAAATTCA GAACTCATAC TTACTGAAGC TAAACAAAAA
    ATGAGAGAAC TTACTATTAA CATCAAGATG AAGGAAGATC TGATTAAAGA ATTAATTAAA
    ACAGGTAATG ATGCCAAGTC CGTAAGCAAG CAGTATTCTC TGAAAGTAAC AAAACTTGAA
    CATGAAGCAG AACAGGCAAA AGTGGAATTA ACTGAAACAG AAAAGCAACT ACAGGAACTG
    GAAAACAAAG ATCTTTCTGA TGTTGCTTTG AAGGTAAAAT TACAGAAGGA ATTTCGTAGA
    AAGATGGATG CCGCTAAGCT AAGAGTCCAG GTCTTACAGA AGAAGCATCA AAATAGTAAG
    AAATTGGCAT CACTGTCAAT CCAAAATGAG AAACGTGCTA ATGAACTAGA GCAGAATGTA
    GATCACATGA AATATCAAAA GGTACAGCTG CAAAAAAGAC TTCGAGAAGA AAACGAAAAA
    AGGAAACAAC TGGATGCAGA AATTAAGCGG GATCAACAAA AAATCAAAGA ACTACAGTTA
    AAAACAGAAC AGAAAGATCT AAAACTGAAA GCTGAGGACC TTGATGCATT TAAGATGAAA
    AGGAGAAAAG GTTCGTTTGG AAGTATAGAC CAACTCCAGA AATTGGATAA GCAAAAGAAA
    TGGTTAGATG AAGAAGTAGA GAAAGTTCTG AACCAACGCC AAGAATTAGA GGAGCTGGAA
    GAAGACTTAA AGAAACGGGA GGCCATAGTT TCAAAGAAGG AGGCCCTGTT ACAGGAGAAG
    AGCCACCTGG AAAGTAAGAA GTTGCGATCT AGTCAGGCTT TGAACACAGA CAGTTTAAAA
    ATATCAACTC GCCTGAGCTT GTTGGACCAA GAGTTGTCTG AAAAGAATGT GCAGCTCCAG
    AGCAGCACAG CTGAGGAGAA AATAAAGATT TCAGAACAAG TACAAGCCCT CCAGAGAGAA
    AAGGATCAGC TGCAGAGACA AAGAAACAGC GTGGATGAGA AACTTAAAAA TGGTAGCGTG
    TTGTCACCTG AAGAAGAACA TGTTCTTTTC CAACTTGAAG AAGGGATTGA AGCTTTGGAA
    GCCGCAATTG AATACAAGAA CGAAAGTATC CAGAGTCGCC AAAACTTGCT CAGAGCTTCA
    TTTCAAAACC TCTCTCATAG TGAAGCAAAT GTCTTAGAAA AACTAATTTG CCTGAGTCCT
    GTTGAGATTA GAGCTGTTCT TTTCAGATAT TTCAATAAGG TGGTTAATTT GCGAGAAGTT
    GAACGGAAAC TAAAGTTACA GAATGAAGAA ATTAAAATGA AAGTTCTGGA ACGGGATAAC
    ATGGTTCGTG AGTTAGAATC TGCACTGGAA CATCTGAAAT TTCAGTGTGA CCGGAGACTG
    ATCCTCCAGC AAAAGGAACA TGAGCAAAAA ATGCAACTGC TGTTACATCA TTTCAAAGAG
    CGAGATGGGG AAGGCATTGT GGAAACTTTA AAAAATTATG AAGATAAAAT CCATCAGCTG
    GAAAGAGATC TATATTTCTA TAAGAAAACC AGCAGAGATC TGAAGAAGAA ACTCAAGGAA
    CTGGTAGGGG AAGCAATTCG GCGGCAACTA CTACCATCAG AAAATCATGA TGCTGGAGAT
    GGAGTTCAGA ACCCAGAAAA GGCCGGCATG GTTTCAGAAG AATTAAAATG GGCATCCAGA
    ACTGAAAGTA TGAAATTAAG TGGAAGGGAA AGACCATTAG ACAGTTCCAC AAGCAGTTTA
    AGAACACAAC CAAATCCTCA GAAGCTCTGG GAAGATGGCC CAGAATTAGC TCCAATGTAT
    AGCTCTGTAG CACCCACTAG TGGGCATTTG TTAAGCAATG AGGACAAAAC CGAAATAGAT
    GAAAATCAGT TTACAAAATC TAACAGTCGA CCATCATCCC ACATTCAGCC AGTCGGAAAT
    GTGGGACAGC TGCATGGTGT CACACCTGTA AAGTTGTGTC GCAAAGAGTT ACGCCAGATT
    TCTGCCTTGG AACTATCATT ACGACGTTCC AATCTTGGAG TTGCGGTCGG ATCTATGACT
    GCTGATTCCC TTGAAGTAGC TAGGAAAGTG AGTGACTTAA AAACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.