LOC100497284 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100497284
Entrez Gene ID	100497284
Full Name	calpain-8-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018094102.1	XP_017949591.1	calpain-8-like isoform X5
XM_018094101.1	XP_017949590.1	calpain-8-like isoform X3
XM_002939750.4	XP_002939796.2	calpain-8-like isoform X4
XM_018094100.1	XP_017949589.1	calpain-8-like isoform X1
XM_004914726.3	XP_004914783.1	calpain-8-like isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CAPN8	XP_426117.2
Caenorhabditis elegans (roundworm)	clp-4	NP_497460.2
Caenorhabditis elegans (roundworm)	clp-3	NP_493052.2
Caenorhabditis elegans (roundworm)	clp-7	NP_500082.1
Caenorhabditis elegans (roundworm)	clp-1	NP_498740.2
Mus musculus (house mouse)	Capn8	NP_570960.2
Homo sapiens (human)	CAPN8	NP_001137434.1
Canis lupus familiaris (dog)	CAPN8	XP_854850.3
Caenorhabditis elegans (roundworm)	clp-6	NP_500081.1
Xenopus tropicalis (tropical clawed frog)	capn8	NP_001039108.1
Xenopus tropicalis (tropical clawed frog)	LOC100497284	XP_004914783.1
Rattus norvegicus (Norway rat)	Capn8	NP_579843.2
Danio rerio (zebrafish)	capn8	NP_001231009.1

Gene Ontology

The following LOC100497284 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100497284 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10815	XM_004914726.3 Latest version!	Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa10816	XM_002939750.4 Latest version!	Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa44550	XM_018094100.1 Latest version!	Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa44551	XM_018094101.1 Latest version!	Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa44552	XM_018094102.1 Latest version!	Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100497284 ( XM_004914726.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914783.1 Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10815
Clone ID Related Accession (Same CDS sequence)	XM_004914726.3
Accession Version	XM_004914726.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpain-8-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914726.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGAA GTGCTGCCCT GATAGCCAAG GAGCGGTCTG CGGCCAATGT GGGGCGAACG 
AAGAGGAACC CAGTGAAGTA TTTGAATCAA GACTTTGATA AGCTGAGGGC ACAGTGTTTG 
GCATCTGGGA CTCTCTTCAG TGATGAGGAA TTCCCAGCAT GCCCATCTTC CCTGGGTTAC 
AATGAACTGG GACCCGGCTC ATCCAAAACC AATGGAATTG TATGGAAGAG ACCCAAGGAG 
ATTTGCCCCA ACGGCCCCCA GTTTATTGTA GATGGAGCAA CGCGTGGAGA CATCCGTCAG 
GGGACACTTG GGGACTGCTG GCTCCTGGCG GCCATCGCAT CTCTTACACT GGATCCAGAC 
CTTGTAGCCC AGGTGGTTCC AGAGAACCAA AGTTTCCAGA AGGGCTACGC TGGGATCTTC 
CACTTCCAGT TCTGGCAGTA CGGAGAGTGG GTGGACGTGG TGGTGGATGA CCGGCTGCCT 
ATGAAGGATG GGAACCTGGT GTTTGTACAC TCGGCTGAAG GGGACGAATT CTGGAGCGCT 
CTGCTAGAGA AGGCCTACGC AAAGTTGAAT GGCTCCTACG AGGCTCTGAC TGGCGGTTTG 
ACCATAGAGG GATTTGAAGA CTTTACCGGA GGTATCGCCG AGGTGTATGA ACTGAACGCG 
GCTCCGTCTG ATTTATTCCA GATCATCCAA AAAGCCCTTA GGGCTGAATC ACTGCTTGGC 
TGCTCTATTG ACATTTCTAA TTCAAATGAC ACTGAAGCCG AGACAAGCAG AAAGCTGGTT 
AAGGGGCACG CGTATTCAGT GACGGGCGCC GAGGAGGTGT TATACCGAGG GCGCCGGGAG 
AAGCTGATCC GAGTGAGAAA TCCCTGGGGA GAAGTAGAAT GGACCGGACC TTGGAGTGAT 
TATGCTCCAG AGTGGAATTA TATTGATCCT AAGGTGAAGG CAGCTCTGGA TAAACAGTCT 
GACGATGGAG AATTTTGGAT GGCGTTTTCT GACTTTCTCC GAGAGTATTC CCGCCTGGAG 
ATCTGTAACC TGTCCCCCGA CACACTCACC AGCACCGAGC AGCATAAATG GAACATAACC 
CTGTACAATG GGAGCTGGGC ACGGGGGTCT ACTGCCGGCG GCTGCCCCAA ATACCCAGAA 
ACATTCTGGA CAAACCCACA GTTTCGCATT AAACTGGATA CGCCGGATTG GGGCCACAAG 
GGGTCGGATA ATGAGCCCTG CTGTACAGTC ATTGTGGGGC TGATGCAGAA GAACCGCAGA 
CGGAACAGAA AGATGGGGGA GGATTTGCTT AGCATCGGCT TCTCCATATT TAGGATCCCT 
GATCAGCTTC GAGGCCACAC TGATAGCAAC TTCAGCAGGG AATTCTTCCA AAGGTCTCGA 
ACAGTGGCTC GATCTGACGC CTATATAAAC ATACGGGAGG TCTCCCAGCG CTTCTATCTG 
CCAGTCGGGG ATTATCTCAT CGTCCCAACC ACCTTTGAGC CTTTCCAAAA TGGCGACTTC 
TGCCTTCGAA TCTTCTCAGA AAAGGAGGCC AAATCTCTGG AAGTCGGAAA TGTGGTGACA 
GTAAATCCAT ATGAGCCTCA GATTTCTAAA AGGGCCACTG ATGAACTCAA GGAGATGTTT 
TATGAGTTTG CCGGAGAAAA AGAAGAACTT GATGCTACAG ATCTCCAAAG CATTCTCAAT 
GTGCTCCTAT CAATCCGTGA TCGTTGCCCA GAAACGCCAC GTGCAACTGA CAAGCACTTA 
CACAAGAAAC GGAGTTGTTT TCAGGGACTT TGGTGCCACA TTGGGCGCTC CTCTGAAACG 
CGGCGCTCAA AGCGACTAGA AACACCCCCC GCGCCACATC CCACAAGGCC GGACTTGAGA 
TCGGAGAAAT TTACCCTTAG CACTTGCAGG GAGATGATCA GCCTCCAGGA TATGGATGGG 
ACAGAAACAC TGAACCTCCG GGAGTTCCGA GTTCTCTGGA TGAAGATACA GAAATACATG 
ACAATCTATT TAAGGGCGGA CTCGGATCGT TCTGGAACCA TAGACGCTCT TGAGCTGAGA 
ACAGCTTTAC CACAAGCAGG TTTTACTCTG AATAATAAGA TCCAGCAGCG CATTGTGAAG 
CGATACGCCT CTGAGGACCT GGCTATTAAC TTAGATGCCT TTATTGCCTG TATGATGCGC 
CTGGAGACCC TGTTCAAAAT GTTCGAGATA TTGGACAAGA GCAAGAGAGG GGTCGTTGAG 
CTGGATTTAC AAGAGTGGCT CTGTGCAACA CTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914783.1
CDS	267..2525
Translation	MSRSAALIAKERSAANVGRTKRNPVKYLNQDFDKLRAQCLASGTLFSDEEFPACPSSLGYNELGPGSSKTNGIVWKRPKEICPNGPQFIVDGATRGDIRQGTLGDCWLLAAIASLTLDPDLVAQVVPENQSFQKGYAGIFHFQFWQYGEWVDVVVDDRLPMKDGNLVFVHSAEGDEFWSALLEKAYAKLNGSYEALTGGLTIEGFEDFTGGIAEVYELNAAPSDLFQIIQKALRAESLLGCSIDISNSNDTEAETSRKLVKGHAYSVTGAEEVLYRGRREKLIRVRNPWGEVEWTGPWSDYAPEWNYIDPKVKAALDKQSDDGEFWMAFSDFLREYSRLEICNLSPDTLTSTEQHKWNITLYNGSWARGSTAGGCPKYPETFWTNPQFRIKLDTPDWGHKGSDNEPCCTVIVGLMQKNRRRNRKMGEDLLSIGFSIFRIPDQLRGHTDSNFSREFFQRSRTVARSDAYINIREVSQRFYLPVGDYLIVPTTFEPFQNGDFCLRIFSEKEAKSLEVGNVVTVNPYEPQISKRATDELKEMFYEFAGEKEELDATDLQSILNVLLSIRDRCPETPRATDKHLHKKRSCFQGLWCHIGRSSETRRSKRLETPPAPHPTRPDLRSEKFTLSTCREMISLQDMDGTETLNLREFRVLWMKIQKYMTIYLRADSDRSGTIDALELRTALPQAGFTLNNKIQQRIVKRYASEDLAINLDAFIACMMRLETLFKMFEILDKSKRGVVELDLQEWLCATLI

Target ORF information:

RefSeq Version	XM_004914726.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914726.3

ORF Insert Sequence:

ATGTCGAGAA GTGCTGCCCT GATAGCCAAG GAGCGGTCTG CGGCCAATGT GGGGCGAACG 
AAGAGGAACC CAGTGAAGTA TTTGAATCAA GACTTTGATA AGCTGAGGGC ACAGTGTTTG 
GCATCTGGGA CTCTCTTCAG TGATGAGGAA TTCCCAGCAT GCCCATCTTC CCTGGGTTAC 
AATGAACTGG GACCCGGCTC ATCCAAAACC AATGGAATTG TATGGAAGAG ACCCAAGGAG 
ATTTGCCCCA ACGGCCCCCA GTTTATTGTA GATGGAGCAA CGCGTGGAGA CATCCGTCAG 
GGGACACTTG GGGACTGCTG GCTCCTGGCG GCCATCGCAT CTCTTACACT GGATCCAGAC 
CTTGTAGCCC AGGTGGTTCC AGAGAACCAA AGTTTCCAGA AGGGCTACGC TGGGATCTTC 
CACTTCCAGT TCTGGCAGTA CGGAGAGTGG GTGGACGTGG TGGTGGATGA CCGGCTGCCT 
ATGAAGGATG GGAACCTGGT GTTTGTACAC TCGGCTGAAG GGGACGAATT CTGGAGCGCT 
CTGCTAGAGA AGGCCTACGC AAAGTTGAAT GGCTCCTACG AGGCTCTGAC TGGCGGTTTG 
ACCATAGAGG GATTTGAAGA CTTTACCGGA GGTATCGCCG AGGTGTATGA ACTGAACGCG 
GCTCCGTCTG ATTTATTCCA GATCATCCAA AAAGCCCTTA GGGCTGAATC ACTGCTTGGC 
TGCTCTATTG ACATTTCTAA TTCAAATGAC ACTGAAGCCG AGACAAGCAG AAAGCTGGTT 
AAGGGGCACG CGTATTCAGT GACGGGCGCC GAGGAGGTGT TATACCGAGG GCGCCGGGAG 
AAGCTGATCC GAGTGAGAAA TCCCTGGGGA GAAGTAGAAT GGACCGGACC TTGGAGTGAT 
TATGCTCCAG AGTGGAATTA TATTGATCCT AAGGTGAAGG CAGCTCTGGA TAAACAGTCT 
GACGATGGAG AATTTTGGAT GGCGTTTTCT GACTTTCTCC GAGAGTATTC CCGCCTGGAG 
ATCTGTAACC TGTCCCCCGA CACACTCACC AGCACCGAGC AGCATAAATG GAACATAACC 
CTGTACAATG GGAGCTGGGC ACGGGGGTCT ACTGCCGGCG GCTGCCCCAA ATACCCAGAA 
ACATTCTGGA CAAACCCACA GTTTCGCATT AAACTGGATA CGCCGGATTG GGGCCACAAG 
GGGTCGGATA ATGAGCCCTG CTGTACAGTC ATTGTGGGGC TGATGCAGAA GAACCGCAGA 
CGGAACAGAA AGATGGGGGA GGATTTGCTT AGCATCGGCT TCTCCATATT TAGGATCCCT 
GATCAGCTTC GAGGCCACAC TGATAGCAAC TTCAGCAGGG AATTCTTCCA AAGGTCTCGA 
ACAGTGGCTC GATCTGACGC CTATATAAAC ATACGGGAGG TCTCCCAGCG CTTCTATCTG 
CCAGTCGGGG ATTATCTCAT CGTCCCAACC ACCTTTGAGC CTTTCCAAAA TGGCGACTTC 
TGCCTTCGAA TCTTCTCAGA AAAGGAGGCC AAATCTCTGG AAGTCGGAAA TGTGGTGACA 
GTAAATCCAT ATGAGCCTCA GATTTCTAAA AGGGCCACTG ATGAACTCAA GGAGATGTTT 
TATGAGTTTG CCGGAGAAAA AGAAGAACTT GATGCTACAG ATCTCCAAAG CATTCTCAAT 
GTGCTCCTAT CAATCCGTGA TCGTTGCCCA GAAACGCCAC GTGCAACTGA CAAGCACTTA 
CACAAGAAAC GGAGTTGTTT TCAGGGACTT TGGTGCCACA TTGGGCGCTC CTCTGAAACG 
CGGCGCTCAA AGCGACTAGA AACACCCCCC GCGCCACATC CCACAAGGCC GGACTTGAGA 
TCGGAGAAAT TTACCCTTAG CACTTGCAGG GAGATGATCA GCCTCCAGGA TATGGATGGG 
ACAGAAACAC TGAACCTCCG GGAGTTCCGA GTTCTCTGGA TGAAGATACA GAAATACATG 
ACAATCTATT TAAGGGCGGA CTCGGATCGT TCTGGAACCA TAGACGCTCT TGAGCTGAGA 
ACAGCTTTAC CACAAGCAGG TTTTACTCTG AATAATAAGA TCCAGCAGCG CATTGTGAAG 
CGATACGCCT CTGAGGACCT GGCTATTAAC TTAGATGCCT TTATTGCCTG TATGATGCGC 
CTGGAGACCC TGTTCAAAAT GTTCGAGATA TTGGACAAGA GCAAGAGAGG GGTCGTTGAG 
CTGGATTTAC AAGAGTGGCT CTGTGCAACA CTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100497284 ( XM_002939750.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939796.2 Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa10816
Clone ID Related Accession (Same CDS sequence)	XM_002939750.4
Accession Version	XM_002939750.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpain-8-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939750.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGAA GTGCTGCCCT GATAGCCAAG GAGCGGTCTG CGGCCAATGT GGGGCGAACG 
AAGAGGAACC CAGTGAAGTA TTTGAATCAA GACTTTGATA AGCTGAGGGC ACAGTGTTTG 
GCATCTGGGA CTCTCTTCAG TGATGAGGAA TTCCCAGCAT GCCCATCTTC CCTGGGTTAC 
AATGAACTGG GACCCGGCTC ATCCAAAACC AATGGAATTG TATGGAAGAG ACCCAAGGAG 
ATTTGCCCCA ACGGCCCCCA GTTTATTGTA GATGGAGCAA CGCGTGGAGA CATCCGTCAG 
GGGACACTTG GGGACTGCTG GCTCCTGGCG GCCATCGCAT CTCTTACACT GGATCCAGAC 
CTTGTAGCCC AGGTGGTTCC AGAGAACCAA AGTTTCCAGA AGGGCTACGC TGGGATCTTC 
CACTTCCAGT TCTGGCAGTA CGGAGAGTGG GTGGACGTGG TGGTGGATGA CCGGCTGCCT 
ATGAAGGATG GGAACCTGGT GTTTGTACAC TCGGCTGAAG GGGACGAATT CTGGAGCGCT 
CTGCTAGAGA AGGCCTACGC AAAGTTGAAT GGCTCCTACG AGGCTCTGAC TGGCGGTTTG 
ACCATAGAGG GATTTGAAGA CTTTACCGGA GGTATCGCCG AGGTGTATGA ACTGAACGCG 
GCTCCGTCTG ATTTATTCCA GATCATCCAA AAAGCCCTTA GGGCTGAATC ACTGCTTGGC 
TGCTCTATTG ACATTTCTAA TTCAAATGAC ACTGAAGCCG AGACAAGCAG AAAGCTGGTT 
AAGGGGCACG CGTATTCAGT GACGGGCGCC GAGGAGGTGT TATACCGAGG GCGCCGGGAG 
AAGCTGATCC GAGTGAGAAA TCCCTGGGGA GAAGTAGAAT GGACCGGACC TTGGAGTGAT 
TATGCTCCAG AGTGGAATTA TATTGATCCT AAGGTGAAGG CAGCTCTGGA TAAACAGTCT 
GACGATGGAG AATTTTGGAT GGCGTTTTCT GACTTTCTCC GAGAGTATTC CCGCCTGGAG 
ATCTGTAACC TGTCCCCCGA CACACTCACC AGCACCGAGC AGCATAAATG GAACATAACC 
CTGTACAATG GGAGCTGGGC ACGGGGGTCT ACTGCCGGCG GCTGCCCCAA ATACCCAGAA 
ACATTCTGGA CAAACCCACA GTTTCGCATT AAACTGGATA CGCCGGATTG GGGCCACAAG 
GGGTCGGATA ATGAGCCCTG CTGTACAGTC ATTGTGGGGC TGATGCAGAA GAACCGCAGA 
CGGAACAGAA AGATGGGGGA GGATTTGCTT AGCATCGGCT TCTCCATATT TAGGATCCCT 
GATCAGCTTC GAGGCCACAC TGATAGCAAC TTCAGCAGGG AATTCTTCCA AAGGTCTCGA 
ACAGTGGCTC GATCTGACGC CTATATAAAC ATACGGGAGG TCTCCCAGCG CTTCTATCTG 
CCAGTCGGGG ATTATCTCAT CGTCCCAACC ACCTTTGAGC CTTTCCAAAA TGGCGACTTC 
TGCCTTCGAA TCTTCTCAGA AAAGGAGGCC AAATCTCTGG AAGTCGGAAA TGTGGTGACA 
GTAAATCCAT ATGAGCCTCA GATTTCTAAA AGGGCCACTG ATGAACTCAA GGAGATGTTT 
TATGAGTTTG CCGGAGAAAA AGAAGAACTT GATGCTACAG ATCTCCAAAG CATTCTCAAT 
GTGCTCCTAT CAATCCGGCC GGACTTGAGA TCGGAGAAAT TTACCCTTAG CACTTGCAGG 
GAGATGATCA GCCTCCAGGA TATGGATGGG ACAGAAACAC TGAACCTCCG GGAGTTCCGA 
GTTCTCTGGA TGAAGATACA GAAATACATG ACAATCTATT TAAGGGCGGA CTCGGATCGT 
TCTGGAACCA TAGACGCTCT TGAGCTGAGA ACAGCTTTAC CACAAGCAGG TTTTACTCTG 
AATAATAAGA TCCAGCAGCG CATTGTGAAG CGATACGCCT CTGAGGACCT GGCTATTAAC 
TTAGATGCCT TTATTGCCTG TATGATGCGC CTGGAGACCC TGTTCAAAAT GTTCGAGATA 
TTGGACAAGA GCAAGAGAGG GGTCGTTGAG CTGGATTTAC AAGAGTGGCT CTGTGCAACA 
CTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939796.2
CDS	264..2372
Translation	MSRSAALIAKERSAANVGRTKRNPVKYLNQDFDKLRAQCLASGTLFSDEEFPACPSSLGYNELGPGSSKTNGIVWKRPKEICPNGPQFIVDGATRGDIRQGTLGDCWLLAAIASLTLDPDLVAQVVPENQSFQKGYAGIFHFQFWQYGEWVDVVVDDRLPMKDGNLVFVHSAEGDEFWSALLEKAYAKLNGSYEALTGGLTIEGFEDFTGGIAEVYELNAAPSDLFQIIQKALRAESLLGCSIDISNSNDTEAETSRKLVKGHAYSVTGAEEVLYRGRREKLIRVRNPWGEVEWTGPWSDYAPEWNYIDPKVKAALDKQSDDGEFWMAFSDFLREYSRLEICNLSPDTLTSTEQHKWNITLYNGSWARGSTAGGCPKYPETFWTNPQFRIKLDTPDWGHKGSDNEPCCTVIVGLMQKNRRRNRKMGEDLLSIGFSIFRIPDQLRGHTDSNFSREFFQRSRTVARSDAYINIREVSQRFYLPVGDYLIVPTTFEPFQNGDFCLRIFSEKEAKSLEVGNVVTVNPYEPQISKRATDELKEMFYEFAGEKEELDATDLQSILNVLLSIRPDLRSEKFTLSTCREMISLQDMDGTETLNLREFRVLWMKIQKYMTIYLRADSDRSGTIDALELRTALPQAGFTLNNKIQQRIVKRYASEDLAINLDAFIACMMRLETLFKMFEILDKSKRGVVELDLQEWLCATLI

Target ORF information:

RefSeq Version	XM_002939750.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939750.4

ORF Insert Sequence:

ATGTCGAGAA GTGCTGCCCT GATAGCCAAG GAGCGGTCTG CGGCCAATGT GGGGCGAACG 
AAGAGGAACC CAGTGAAGTA TTTGAATCAA GACTTTGATA AGCTGAGGGC ACAGTGTTTG 
GCATCTGGGA CTCTCTTCAG TGATGAGGAA TTCCCAGCAT GCCCATCTTC CCTGGGTTAC 
AATGAACTGG GACCCGGCTC ATCCAAAACC AATGGAATTG TATGGAAGAG ACCCAAGGAG 
ATTTGCCCCA ACGGCCCCCA GTTTATTGTA GATGGAGCAA CGCGTGGAGA CATCCGTCAG 
GGGACACTTG GGGACTGCTG GCTCCTGGCG GCCATCGCAT CTCTTACACT GGATCCAGAC 
CTTGTAGCCC AGGTGGTTCC AGAGAACCAA AGTTTCCAGA AGGGCTACGC TGGGATCTTC 
CACTTCCAGT TCTGGCAGTA CGGAGAGTGG GTGGACGTGG TGGTGGATGA CCGGCTGCCT 
ATGAAGGATG GGAACCTGGT GTTTGTACAC TCGGCTGAAG GGGACGAATT CTGGAGCGCT 
CTGCTAGAGA AGGCCTACGC AAAGTTGAAT GGCTCCTACG AGGCTCTGAC TGGCGGTTTG 
ACCATAGAGG GATTTGAAGA CTTTACCGGA GGTATCGCCG AGGTGTATGA ACTGAACGCG 
GCTCCGTCTG ATTTATTCCA GATCATCCAA AAAGCCCTTA GGGCTGAATC ACTGCTTGGC 
TGCTCTATTG ACATTTCTAA TTCAAATGAC ACTGAAGCCG AGACAAGCAG AAAGCTGGTT 
AAGGGGCACG CGTATTCAGT GACGGGCGCC GAGGAGGTGT TATACCGAGG GCGCCGGGAG 
AAGCTGATCC GAGTGAGAAA TCCCTGGGGA GAAGTAGAAT GGACCGGACC TTGGAGTGAT 
TATGCTCCAG AGTGGAATTA TATTGATCCT AAGGTGAAGG CAGCTCTGGA TAAACAGTCT 
GACGATGGAG AATTTTGGAT GGCGTTTTCT GACTTTCTCC GAGAGTATTC CCGCCTGGAG 
ATCTGTAACC TGTCCCCCGA CACACTCACC AGCACCGAGC AGCATAAATG GAACATAACC 
CTGTACAATG GGAGCTGGGC ACGGGGGTCT ACTGCCGGCG GCTGCCCCAA ATACCCAGAA 
ACATTCTGGA CAAACCCACA GTTTCGCATT AAACTGGATA CGCCGGATTG GGGCCACAAG 
GGGTCGGATA ATGAGCCCTG CTGTACAGTC ATTGTGGGGC TGATGCAGAA GAACCGCAGA 
CGGAACAGAA AGATGGGGGA GGATTTGCTT AGCATCGGCT TCTCCATATT TAGGATCCCT 
GATCAGCTTC GAGGCCACAC TGATAGCAAC TTCAGCAGGG AATTCTTCCA AAGGTCTCGA 
ACAGTGGCTC GATCTGACGC CTATATAAAC ATACGGGAGG TCTCCCAGCG CTTCTATCTG 
CCAGTCGGGG ATTATCTCAT CGTCCCAACC ACCTTTGAGC CTTTCCAAAA TGGCGACTTC 
TGCCTTCGAA TCTTCTCAGA AAAGGAGGCC AAATCTCTGG AAGTCGGAAA TGTGGTGACA 
GTAAATCCAT ATGAGCCTCA GATTTCTAAA AGGGCCACTG ATGAACTCAA GGAGATGTTT 
TATGAGTTTG CCGGAGAAAA AGAAGAACTT GATGCTACAG ATCTCCAAAG CATTCTCAAT 
GTGCTCCTAT CAATCCGGCC GGACTTGAGA TCGGAGAAAT TTACCCTTAG CACTTGCAGG 
GAGATGATCA GCCTCCAGGA TATGGATGGG ACAGAAACAC TGAACCTCCG GGAGTTCCGA 
GTTCTCTGGA TGAAGATACA GAAATACATG ACAATCTATT TAAGGGCGGA CTCGGATCGT 
TCTGGAACCA TAGACGCTCT TGAGCTGAGA ACAGCTTTAC CACAAGCAGG TTTTACTCTG 
AATAATAAGA TCCAGCAGCG CATTGTGAAG CGATACGCCT CTGAGGACCT GGCTATTAAC 
TTAGATGCCT TTATTGCCTG TATGATGCGC CTGGAGACCC TGTTCAAAAT GTTCGAGATA 
TTGGACAAGA GCAAGAGAGG GGTCGTTGAG CTGGATTTAC AAGAGTGGCT CTGTGCAACA 
CTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100497284 ( XM_018094100.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949589.1 Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa44550
Clone ID Related Accession (Same CDS sequence)	XM_018094100.1
Accession Version	XM_018094100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpain-8-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGAA GTGCTGCCCT GATAGCCAAG GAGCGGTCTG CGGCCAATGT GGGGCGAACG 
AAGAGGAACC CAGTGAAGTA TTTGAATCAA GACTTTGATA AGCTGAGGGC ACAGTGTTTG 
GCATCTGGGA CTCTCTTCAG TGATGAGGAA TTCCCAGCAT GCCCATCTTC CCTGGGTTAC 
AATGAACTGG GACCCGGCTC ATCCAAAACC AATGGAATTG TATGGAAGAG ACCCAAGGAG 
ATTTGCCCCA ACGGCCCCCA GTTTATTGTA GATGGAGCAA CGCGTGGAGA CATCCGTCAG 
GGGACACTTG GGGACTGCTG GCTCCTGGCG GCCATCGCAT CTCTTACACT GGATCCAGAC 
CTTGTAGCCC AGGTGGTTCC AGAGAACCAA AGTTTCCAGA AGGGCTACGC TGGGATCTTC 
CACTTCCAGT TCTGGCAGTA CGGAGAGTGG GTGGACGTGG TGGTGGATGA CCGGCTGCCT 
ATGAAGGATG GGAACCTGGT GTTTGTACAC TCGGCTGAAG GGGACGAATT CTGGAGCGCT 
CTGCTAGAGA AGGCCTACGC AAAGTTGAAT GGCTCCTACG AGGCTCTGAC TGGCGGTTTG 
ACCATAGAGG GATTTGAAGA CTTTACCGGA GGTATCGCCG AGGTGTATGA ACTGAACGCG 
GCTCCGTCTG ATTTATTCCA GATCATCCAA AAAGCCCTTA GGGCTGAATC ACTGCTTGGC 
TGCTCTATTG ACATTTCTAA TTCAAATGAC ACTGAAGCCG AGACAAGCAG AAAGCTGGTT 
AAGGGGCACG CGTATTCAGT GACGGGCGCC GAGGAGGTGT TATACCGAGG GCGCCGGGAG 
AAGCTGATCC GAGTGAGAAA TCCCTGGGGA GAAGTAGAAT GGACCGGACC TTGGAGTGAT 
TATGCTCCAG AGTGGAATTA TATTGATCCT AAGGTGAAGG CAGCTCTGGA TAAACAGTCT 
GACGATGGAG AATTTTGGAT GGCGTTTTCT GACTTTCTCC GAGAGTATTC CCGCCTGGAG 
ATCTGTAACC TGTCCCCCGA CACACTCACC AGCACCGAGC AGCATAAATG GAACATAACC 
CTGTACAATG GGAGCTGGGC ACGGGGGTCT ACTGCCGGCG GCTGCCCCAA ATACCCAGAA 
ACATTCTGGA CAAACCCACA GTTTCGCATT AAACTGGATA CGCCGGATTG GGGCCACAAG 
GGGTCGGATA ATGAGCCCTG CTGTACAGTC ATTGTGGGGC TGATGCAGAA GAACCGCAGA 
CGGAACAGAA AGATGGGGGA GGATTTGCTT AGCATCGGCT TCTCCATATT TAGGATCCCT 
GATCAGCTTC GAGGCCACAC TGATAGCAAC TTCAGCAGGG AATTCTTCCA AAGGTCTCGA 
ACAGTGGCTC GATCTGACGC CTATATAAAC ATACGGGAGG TCTCCCAGCG CTTCTATCTG 
CCAGTCGGGG ATTATCTCAT CGTCCCAACC ACCTTTGAGC CTTTCCAAAA TGGCGACTTC 
TGCCTTCGAA TCTTCTCAGA AAAGGAGGCC AAATCTCTGG AAGTCGGAAA TGTGGTGACA 
GTAAATCCAT ATGAGCCTCA GATTTCTAAA AGGGCCACTG ATGAACTCAA GGAGATGTTT 
TATGAGTTTG CCGGAGAAGT AAGAACATTT TCCCCTAATC TGACAGTACA TTATAAAGAA 
GAACTTGATG CTACAGATCT CCAAAGCATT CTCAATGTGC TCCTATCAAT CCGTGATCGT 
TGCCCAGAAA CGCCACGTGC AACTGACAAG CACTTACACA AGAAACGGAG TTGTTTTCAG 
GGACTTTGGT GCCACATTGG GCGCTCCTCT GAAACGCGGC GCTCAAAGCG ACTAGAAACA 
CCCCCCGCGC CACATCCCAC AAGGCCGGAC TTGAGATCGG AGAAATTTAC CCTTAGCACT 
TGCAGGGAGA TGATCAGCCT CCAGGATATG GATGGGACAG AAACACTGAA CCTCCGGGAG 
TTCCGAGTTC TCTGGATGAA GATACAGAAA TACATGACAA TCTATTTAAG GGCGGACTCG 
GATCGTTCTG GAACCATAGA CGCTCTTGAG CTGAGAACAG CTTTACCACA AGCAGGTTTT 
ACTCTGAATA ATAAGATCCA GCAGCGCATT GTGAAGCGAT ACGCCTCTGA GGACCTGGCT 
ATTAACTTAG ATGCCTTTAT TGCCTGTATG ATGCGCCTGG AGACCCTGTT CAAAATGTTC 
GAGATATTGG ACAAGAGCAA GAGAGGGGTC GTTGAGCTGG ATTTACAAGA GTGGCTCTGT 
GCAACACTGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949589.1
CDS	269..2563
Translation	MSRSAALIAKERSAANVGRTKRNPVKYLNQDFDKLRAQCLASGTLFSDEEFPACPSSLGYNELGPGSSKTNGIVWKRPKEICPNGPQFIVDGATRGDIRQGTLGDCWLLAAIASLTLDPDLVAQVVPENQSFQKGYAGIFHFQFWQYGEWVDVVVDDRLPMKDGNLVFVHSAEGDEFWSALLEKAYAKLNGSYEALTGGLTIEGFEDFTGGIAEVYELNAAPSDLFQIIQKALRAESLLGCSIDISNSNDTEAETSRKLVKGHAYSVTGAEEVLYRGRREKLIRVRNPWGEVEWTGPWSDYAPEWNYIDPKVKAALDKQSDDGEFWMAFSDFLREYSRLEICNLSPDTLTSTEQHKWNITLYNGSWARGSTAGGCPKYPETFWTNPQFRIKLDTPDWGHKGSDNEPCCTVIVGLMQKNRRRNRKMGEDLLSIGFSIFRIPDQLRGHTDSNFSREFFQRSRTVARSDAYINIREVSQRFYLPVGDYLIVPTTFEPFQNGDFCLRIFSEKEAKSLEVGNVVTVNPYEPQISKRATDELKEMFYEFAGEVRTFSPNLTVHYKEELDATDLQSILNVLLSIRDRCPETPRATDKHLHKKRSCFQGLWCHIGRSSETRRSKRLETPPAPHPTRPDLRSEKFTLSTCREMISLQDMDGTETLNLREFRVLWMKIQKYMTIYLRADSDRSGTIDALELRTALPQAGFTLNNKIQQRIVKRYASEDLAINLDAFIACMMRLETLFKMFEILDKSKRGVVELDLQEWLCATLI

Target ORF information:

RefSeq Version	XM_018094100.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094100.1

ORF Insert Sequence:

ATGTCGAGAA GTGCTGCCCT GATAGCCAAG GAGCGGTCTG CGGCCAATGT GGGGCGAACG 
AAGAGGAACC CAGTGAAGTA TTTGAATCAA GACTTTGATA AGCTGAGGGC ACAGTGTTTG 
GCATCTGGGA CTCTCTTCAG TGATGAGGAA TTCCCAGCAT GCCCATCTTC CCTGGGTTAC 
AATGAACTGG GACCCGGCTC ATCCAAAACC AATGGAATTG TATGGAAGAG ACCCAAGGAG 
ATTTGCCCCA ACGGCCCCCA GTTTATTGTA GATGGAGCAA CGCGTGGAGA CATCCGTCAG 
GGGACACTTG GGGACTGCTG GCTCCTGGCG GCCATCGCAT CTCTTACACT GGATCCAGAC 
CTTGTAGCCC AGGTGGTTCC AGAGAACCAA AGTTTCCAGA AGGGCTACGC TGGGATCTTC 
CACTTCCAGT TCTGGCAGTA CGGAGAGTGG GTGGACGTGG TGGTGGATGA CCGGCTGCCT 
ATGAAGGATG GGAACCTGGT GTTTGTACAC TCGGCTGAAG GGGACGAATT CTGGAGCGCT 
CTGCTAGAGA AGGCCTACGC AAAGTTGAAT GGCTCCTACG AGGCTCTGAC TGGCGGTTTG 
ACCATAGAGG GATTTGAAGA CTTTACCGGA GGTATCGCCG AGGTGTATGA ACTGAACGCG 
GCTCCGTCTG ATTTATTCCA GATCATCCAA AAAGCCCTTA GGGCTGAATC ACTGCTTGGC 
TGCTCTATTG ACATTTCTAA TTCAAATGAC ACTGAAGCCG AGACAAGCAG AAAGCTGGTT 
AAGGGGCACG CGTATTCAGT GACGGGCGCC GAGGAGGTGT TATACCGAGG GCGCCGGGAG 
AAGCTGATCC GAGTGAGAAA TCCCTGGGGA GAAGTAGAAT GGACCGGACC TTGGAGTGAT 
TATGCTCCAG AGTGGAATTA TATTGATCCT AAGGTGAAGG CAGCTCTGGA TAAACAGTCT 
GACGATGGAG AATTTTGGAT GGCGTTTTCT GACTTTCTCC GAGAGTATTC CCGCCTGGAG 
ATCTGTAACC TGTCCCCCGA CACACTCACC AGCACCGAGC AGCATAAATG GAACATAACC 
CTGTACAATG GGAGCTGGGC ACGGGGGTCT ACTGCCGGCG GCTGCCCCAA ATACCCAGAA 
ACATTCTGGA CAAACCCACA GTTTCGCATT AAACTGGATA CGCCGGATTG GGGCCACAAG 
GGGTCGGATA ATGAGCCCTG CTGTACAGTC ATTGTGGGGC TGATGCAGAA GAACCGCAGA 
CGGAACAGAA AGATGGGGGA GGATTTGCTT AGCATCGGCT TCTCCATATT TAGGATCCCT 
GATCAGCTTC GAGGCCACAC TGATAGCAAC TTCAGCAGGG AATTCTTCCA AAGGTCTCGA 
ACAGTGGCTC GATCTGACGC CTATATAAAC ATACGGGAGG TCTCCCAGCG CTTCTATCTG 
CCAGTCGGGG ATTATCTCAT CGTCCCAACC ACCTTTGAGC CTTTCCAAAA TGGCGACTTC 
TGCCTTCGAA TCTTCTCAGA AAAGGAGGCC AAATCTCTGG AAGTCGGAAA TGTGGTGACA 
GTAAATCCAT ATGAGCCTCA GATTTCTAAA AGGGCCACTG ATGAACTCAA GGAGATGTTT 
TATGAGTTTG CCGGAGAAGT AAGAACATTT TCCCCTAATC TGACAGTACA TTATAAAGAA 
GAACTTGATG CTACAGATCT CCAAAGCATT CTCAATGTGC TCCTATCAAT CCGTGATCGT 
TGCCCAGAAA CGCCACGTGC AACTGACAAG CACTTACACA AGAAACGGAG TTGTTTTCAG 
GGACTTTGGT GCCACATTGG GCGCTCCTCT GAAACGCGGC GCTCAAAGCG ACTAGAAACA 
CCCCCCGCGC CACATCCCAC AAGGCCGGAC TTGAGATCGG AGAAATTTAC CCTTAGCACT 
TGCAGGGAGA TGATCAGCCT CCAGGATATG GATGGGACAG AAACACTGAA CCTCCGGGAG 
TTCCGAGTTC TCTGGATGAA GATACAGAAA TACATGACAA TCTATTTAAG GGCGGACTCG 
GATCGTTCTG GAACCATAGA CGCTCTTGAG CTGAGAACAG CTTTACCACA AGCAGGTTTT 
ACTCTGAATA ATAAGATCCA GCAGCGCATT GTGAAGCGAT ACGCCTCTGA GGACCTGGCT 
ATTAACTTAG ATGCCTTTAT TGCCTGTATG ATGCGCCTGG AGACCCTGTT CAAAATGTTC 
GAGATATTGG ACAAGAGCAA GAGAGGGGTC GTTGAGCTGG ATTTACAAGA GTGGCTCTGT 
GCAACACTGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100497284 ( XM_018094101.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949590.1 Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X3, mRNA.

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CloneID	OXa44551
Clone ID Related Accession (Same CDS sequence)	XM_018094101.1
Accession Version	XM_018094101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpain-8-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGAA GTGCTGCCCT GATAGCCAAG GAGCGGTCTG CGGCCAATGT GGGGCGAACG 
AAGAGGAACC CAGTGAAGTA TTTGAATCAA GACTTTGATA AGCTGAGGGC ACAGTGTTTG 
GCATCTGGGA CTCTCTTCAG TGATGAGGAA TTCCCAGCAT GCCCATCTTC CCTGGGTTAC 
AATGAACTGG GACCCGGCTC ATCCAAAACC AATGGAATTG TATGGAAGAG ACCCAAGGAG 
ATTTGCCCCA ACGGCCCCCA GTTTATTGTA GATGGAGCAA CGCGTGGAGA CATCCGTCAG 
GGGACACTTG GGGACTGCTG GCTCCTGGCG GCCATCGCAT CTCTTACACT GGATCCAGAC 
CTTGTAGCCC AGGTGGTTCC AGAGAACCAA AGTTTCCAGA AGGGCTACGC TGGGATCTTC 
CACTTCCAGT TCTGGCAGTA CGGAGAGTGG GTGGACGTGG TGGTGGATGA CCGGCTGCCT 
ATGAAGGATG GGAACCTGGT GTTTGTACAC TCGGCTGAAG GGGACGAATT CTGGAGCGCT 
CTGCTAGAGA AGGCCTACGC AAAGTTGAAT GGCTCCTACG AGGCTCTGAC TGGCGGTTTG 
ACCATAGAGG GATTTGAAGA CTTTACCGGA GGTATCGCCG AGGTGTATGA ACTGAACGCG 
GCTCCGTCTG ATTTATTCCA GATCATCCAA AAAGCCCTTA GGGCTGAATC ACTGCTTGGC 
TGCTCTATTG ACATTTCTAA TTCAAATGAC ACTGAAGCCG AGACAAGCAG AAAGCTGGTT 
AAGGGGCACG CGTATTCAGT GACGGGCGCC GAGGAGGTGT TATACCGAGG GCGCCGGGAG 
AAGCTGATCC GAGTGAGAAA TCCCTGGGGA GAAGTAGAAT GGACCGGACC TTGGAGTGAT 
TATGCTCCAG AGTGGAATTA TATTGATCCT AAGGTGAAGG CAGCTCTGGA TAAACAGTCT 
GACGATGGAG AATTTTGGAT GGCGTTTTCT GACTTTCTCC GAGAGTATTC CCGCCTGGAG 
ATCTGTAACC TGTCCCCCGA CACACTCACC AGCACCGAGC AGCATAAATG GAACATAACC 
CTGTACAATG GGAGCTGGGC ACGGGGGTCT ACTGCCGGCG GCTGCCCCAA ATACCCAGAA 
ACATTCTGGA CAAACCCACA GTTTCGCATT AAACTGGATA CGCCGGATTG GGGCCACAAG 
GGGTCGGATA ATGAGCCCTG CTGTACAGTC ATTGTGGGGC TGATGCAGAA GAACCGCAGA 
CGGAACAGAA AGATGGGGGA GGATTTGCTT AGCATCGGCT TCTCCATATT TAGGATCCCT 
GATCAGCTTC GAGGCCACAC TGATAGCAAC TTCAGCAGGG AATTCTTCCA AAGGTCTCGA 
ACAGTGGCTC GATCTGACGC CTATATAAAC ATACGGGAGG TCTCCCAGCG CTTCTATCTG 
CCAGTCGGGG ATTATCTCAT CGTCCCAACC ACCTTTGAGC CTTTCCAAAA TGGCGACTTC 
TGCCTTCGAA TCTTCTCAGA AAAGGAGGCC AAATCTCTGG AAGTCGGAAA TGTGGTGACA 
GTAAATCCAT ATGAGCCTCA GATTTCTAAA AGGGCCACTG ATGAACTCAA GGAGATGTTT 
TATGAGTTTG CCGGAGAAGT AAGAACATTT TCCCCTAATC TGACAGTACA TTATAAAGAA 
GAACTTGATG CTACAGATCT CCAAAGCATT CTCAATGTGC TCCTATCAAT CCGGCCGGAC 
TTGAGATCGG AGAAATTTAC CCTTAGCACT TGCAGGGAGA TGATCAGCCT CCAGGATATG 
GATGGGACAG AAACACTGAA CCTCCGGGAG TTCCGAGTTC TCTGGATGAA GATACAGAAA 
TACATGACAA TCTATTTAAG GGCGGACTCG GATCGTTCTG GAACCATAGA CGCTCTTGAG 
CTGAGAACAG CTTTACCACA AGCAGGTTTT ACTCTGAATA ATAAGATCCA GCAGCGCATT 
GTGAAGCGAT ACGCCTCTGA GGACCTGGCT ATTAACTTAG ATGCCTTTAT TGCCTGTATG 
ATGCGCCTGG AGACCCTGTT CAAAATGTTC GAGATATTGG ACAAGAGCAA GAGAGGGGTC 
GTTGAGCTGG ATTTACAAGA GTGGCTCTGT GCAACACTGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949590.1
CDS	266..2410
Translation	MSRSAALIAKERSAANVGRTKRNPVKYLNQDFDKLRAQCLASGTLFSDEEFPACPSSLGYNELGPGSSKTNGIVWKRPKEICPNGPQFIVDGATRGDIRQGTLGDCWLLAAIASLTLDPDLVAQVVPENQSFQKGYAGIFHFQFWQYGEWVDVVVDDRLPMKDGNLVFVHSAEGDEFWSALLEKAYAKLNGSYEALTGGLTIEGFEDFTGGIAEVYELNAAPSDLFQIIQKALRAESLLGCSIDISNSNDTEAETSRKLVKGHAYSVTGAEEVLYRGRREKLIRVRNPWGEVEWTGPWSDYAPEWNYIDPKVKAALDKQSDDGEFWMAFSDFLREYSRLEICNLSPDTLTSTEQHKWNITLYNGSWARGSTAGGCPKYPETFWTNPQFRIKLDTPDWGHKGSDNEPCCTVIVGLMQKNRRRNRKMGEDLLSIGFSIFRIPDQLRGHTDSNFSREFFQRSRTVARSDAYINIREVSQRFYLPVGDYLIVPTTFEPFQNGDFCLRIFSEKEAKSLEVGNVVTVNPYEPQISKRATDELKEMFYEFAGEVRTFSPNLTVHYKEELDATDLQSILNVLLSIRPDLRSEKFTLSTCREMISLQDMDGTETLNLREFRVLWMKIQKYMTIYLRADSDRSGTIDALELRTALPQAGFTLNNKIQQRIVKRYASEDLAINLDAFIACMMRLETLFKMFEILDKSKRGVVELDLQEWLCATLI

Target ORF information:

RefSeq Version	XM_018094101.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094101.1

ORF Insert Sequence:

ATGTCGAGAA GTGCTGCCCT GATAGCCAAG GAGCGGTCTG CGGCCAATGT GGGGCGAACG 
AAGAGGAACC CAGTGAAGTA TTTGAATCAA GACTTTGATA AGCTGAGGGC ACAGTGTTTG 
GCATCTGGGA CTCTCTTCAG TGATGAGGAA TTCCCAGCAT GCCCATCTTC CCTGGGTTAC 
AATGAACTGG GACCCGGCTC ATCCAAAACC AATGGAATTG TATGGAAGAG ACCCAAGGAG 
ATTTGCCCCA ACGGCCCCCA GTTTATTGTA GATGGAGCAA CGCGTGGAGA CATCCGTCAG 
GGGACACTTG GGGACTGCTG GCTCCTGGCG GCCATCGCAT CTCTTACACT GGATCCAGAC 
CTTGTAGCCC AGGTGGTTCC AGAGAACCAA AGTTTCCAGA AGGGCTACGC TGGGATCTTC 
CACTTCCAGT TCTGGCAGTA CGGAGAGTGG GTGGACGTGG TGGTGGATGA CCGGCTGCCT 
ATGAAGGATG GGAACCTGGT GTTTGTACAC TCGGCTGAAG GGGACGAATT CTGGAGCGCT 
CTGCTAGAGA AGGCCTACGC AAAGTTGAAT GGCTCCTACG AGGCTCTGAC TGGCGGTTTG 
ACCATAGAGG GATTTGAAGA CTTTACCGGA GGTATCGCCG AGGTGTATGA ACTGAACGCG 
GCTCCGTCTG ATTTATTCCA GATCATCCAA AAAGCCCTTA GGGCTGAATC ACTGCTTGGC 
TGCTCTATTG ACATTTCTAA TTCAAATGAC ACTGAAGCCG AGACAAGCAG AAAGCTGGTT 
AAGGGGCACG CGTATTCAGT GACGGGCGCC GAGGAGGTGT TATACCGAGG GCGCCGGGAG 
AAGCTGATCC GAGTGAGAAA TCCCTGGGGA GAAGTAGAAT GGACCGGACC TTGGAGTGAT 
TATGCTCCAG AGTGGAATTA TATTGATCCT AAGGTGAAGG CAGCTCTGGA TAAACAGTCT 
GACGATGGAG AATTTTGGAT GGCGTTTTCT GACTTTCTCC GAGAGTATTC CCGCCTGGAG 
ATCTGTAACC TGTCCCCCGA CACACTCACC AGCACCGAGC AGCATAAATG GAACATAACC 
CTGTACAATG GGAGCTGGGC ACGGGGGTCT ACTGCCGGCG GCTGCCCCAA ATACCCAGAA 
ACATTCTGGA CAAACCCACA GTTTCGCATT AAACTGGATA CGCCGGATTG GGGCCACAAG 
GGGTCGGATA ATGAGCCCTG CTGTACAGTC ATTGTGGGGC TGATGCAGAA GAACCGCAGA 
CGGAACAGAA AGATGGGGGA GGATTTGCTT AGCATCGGCT TCTCCATATT TAGGATCCCT 
GATCAGCTTC GAGGCCACAC TGATAGCAAC TTCAGCAGGG AATTCTTCCA AAGGTCTCGA 
ACAGTGGCTC GATCTGACGC CTATATAAAC ATACGGGAGG TCTCCCAGCG CTTCTATCTG 
CCAGTCGGGG ATTATCTCAT CGTCCCAACC ACCTTTGAGC CTTTCCAAAA TGGCGACTTC 
TGCCTTCGAA TCTTCTCAGA AAAGGAGGCC AAATCTCTGG AAGTCGGAAA TGTGGTGACA 
GTAAATCCAT ATGAGCCTCA GATTTCTAAA AGGGCCACTG ATGAACTCAA GGAGATGTTT 
TATGAGTTTG CCGGAGAAGT AAGAACATTT TCCCCTAATC TGACAGTACA TTATAAAGAA 
GAACTTGATG CTACAGATCT CCAAAGCATT CTCAATGTGC TCCTATCAAT CCGGCCGGAC 
TTGAGATCGG AGAAATTTAC CCTTAGCACT TGCAGGGAGA TGATCAGCCT CCAGGATATG 
GATGGGACAG AAACACTGAA CCTCCGGGAG TTCCGAGTTC TCTGGATGAA GATACAGAAA 
TACATGACAA TCTATTTAAG GGCGGACTCG GATCGTTCTG GAACCATAGA CGCTCTTGAG 
CTGAGAACAG CTTTACCACA AGCAGGTTTT ACTCTGAATA ATAAGATCCA GCAGCGCATT 
GTGAAGCGAT ACGCCTCTGA GGACCTGGCT ATTAACTTAG ATGCCTTTAT TGCCTGTATG 
ATGCGCCTGG AGACCCTGTT CAAAATGTTC GAGATATTGG ACAAGAGCAA GAGAGGGGTC 
GTTGAGCTGG ATTTACAAGA GTGGCTCTGT GCAACACTGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100497284 ( XM_018094102.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949591.1 Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa44552
Clone ID Related Accession (Same CDS sequence)	XM_018094102.1
Accession Version	XM_018094102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpain-8-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA CCCAAGGGGA CTGCTGGCTC CTGGCGGCCA TCGCATCTCT TACACTGGAT 
CCAGACCTTG TAGCCCAGGT GGTTCCAGAG AACCAAAGTT TCCAGAAGGG CTACGCTGGG 
ATCTTCCACT TCCAGTTCTG GCAGTACGGA GAGTGGGTGG ACGTGGTGGT GGATGACCGG 
CTGCCTATGA AGGATGGGAA CCTGGTGTTT GTACACTCGG CTGAAGGGGA CGAATTCTGG 
AGCGCTCTGC TAGAGAAGGC CTACGCAAAG TTGAATGGCT CCTACGAGGC TCTGACTGGC 
GGTTTGACCA TAGAGGGATT TGAAGACTTT ACCGGAGGTA TCGCCGAGGT GTATGAACTG 
AACGCGGCTC CGTCTGATTT ATTCCAGATC ATCCAAAAAG CCCTTAGGGC TGAATCACTG 
CTTGGCTGCT CTATTGACAT TTCTAATTCA AATGACACTG AAGCCGAGAC AAGCAGAAAG 
CTGGTTAAGG GGCACGCGTA TTCAGTGACG GGCGCCGAGG AGGTGTTATA CCGAGGGCGC 
CGGGAGAAGC TGATCCGAGT GAGAAATCCC TGGGGAGAAG TAGAATGGAC CGGACCTTGG 
AGTGATTATG CTCCAGAGTG GAATTATATT GATCCTAAGG TGAAGGCAGC TCTGGATAAA 
CAGTCTGACG ATGGAGAATT TTGGATGGCG TTTTCTGACT TTCTCCGAGA GTATTCCCGC 
CTGGAGATCT GTAACCTGTC CCCCGACACA CTCACCAGCA CCGAGCAGCA TAAATGGAAC 
ATAACCCTGT ACAATGGGAG CTGGGCACGG GGGTCTACTG CCGGCGGCTG CCCCAAATAC 
CCAGAAACAT TCTGGACAAA CCCACAGTTT CGCATTAAAC TGGATACGCC GGATTGGGGC 
CACAAGGGGT CGGATAATGA GCCCTGCTGT ACAGTCATTG TGGGGCTGAT GCAGAAGAAC 
CGCAGACGGA ACAGAAAGAT GGGGGAGGAT TTGCTTAGCA TCGGCTTCTC CATATTTAGG 
ATCCCTGATC AGCTTCGAGG CCACACTGAT AGCAACTTCA GCAGGGAATT CTTCCAAAGG 
TCTCGAACAG TGGCTCGATC TGACGCCTAT ATAAACATAC GGGAGGTCTC CCAGCGCTTC 
TATCTGCCAG TCGGGGATTA TCTCATCGTC CCAACCACCT TTGAGCCTTT CCAAAATGGC 
GACTTCTGCC TTCGAATCTT CTCAGAAAAG GAGGCCAAAT CTCTGGAAGT CGGAAATGTG 
GTGACAGTAA ATCCATATGA GCCTCAGATT TCTAAAAGGG CCACTGATGA ACTCAAGGAG 
ATGTTTTATG AGTTTGCCGG AGAAGTAAGA ACATTTTCCC CTAATCTGAC AGTACATTAT 
AAAGAAGAAC TTGATGCTAC AGATCTCCAA AGCATTCTCA ATGTGCTCCT ATCAATCCGT 
GATCGTTGCC CAGAAACGCC ACGTGCAACT GACAAGCACT TACACAAGAA ACGGAGTTGT 
TTTCAGGGAC TTTGGTGCCA CATTGGGCGC TCCTCTGAAA CGCGGCGCTC AAAGCGACTA 
GAAACACCCC CCGCGCCACA TCCCACAAGG CCGGACTTGA GATCGGAGAA ATTTACCCTT 
AGCACTTGCA GGGAGATGAT CAGCCTCCAG GATATGGATG GGACAGAAAC ACTGAACCTC 
CGGGAGTTCC GAGTTCTCTG GATGAAGATA CAGAAATACA TGACAATCTA TTTAAGGGCG 
GACTCGGATC GTTCTGGAAC CATAGACGCT CTTGAGCTGA GAACAGCTTT ACCACAAGCA 
GGTTTTACTC TGAATAATAA GATCCAGCAG CGCATTGTGA AGCGATACGC CTCTGAGGAC 
CTGGCTATTA ACTTAGATGC CTTTATTGCC TGTATGATGC GCCTGGAGAC CCTGTTCAAA 
ATGTTCGAGA TATTGGACAA GAGCAAGAGA GGGGTCGTTG AGCTGGATTT ACAAGAGTGG 
CTCTGTGCAA CACTGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949591.1
CDS	89..2089
Translation	MEETQGDCWLLAAIASLTLDPDLVAQVVPENQSFQKGYAGIFHFQFWQYGEWVDVVVDDRLPMKDGNLVFVHSAEGDEFWSALLEKAYAKLNGSYEALTGGLTIEGFEDFTGGIAEVYELNAAPSDLFQIIQKALRAESLLGCSIDISNSNDTEAETSRKLVKGHAYSVTGAEEVLYRGRREKLIRVRNPWGEVEWTGPWSDYAPEWNYIDPKVKAALDKQSDDGEFWMAFSDFLREYSRLEICNLSPDTLTSTEQHKWNITLYNGSWARGSTAGGCPKYPETFWTNPQFRIKLDTPDWGHKGSDNEPCCTVIVGLMQKNRRRNRKMGEDLLSIGFSIFRIPDQLRGHTDSNFSREFFQRSRTVARSDAYINIREVSQRFYLPVGDYLIVPTTFEPFQNGDFCLRIFSEKEAKSLEVGNVVTVNPYEPQISKRATDELKEMFYEFAGEVRTFSPNLTVHYKEELDATDLQSILNVLLSIRDRCPETPRATDKHLHKKRSCFQGLWCHIGRSSETRRSKRLETPPAPHPTRPDLRSEKFTLSTCREMISLQDMDGTETLNLREFRVLWMKIQKYMTIYLRADSDRSGTIDALELRTALPQAGFTLNNKIQQRIVKRYASEDLAINLDAFIACMMRLETLFKMFEILDKSKRGVVELDLQEWLCATLI

Target ORF information:

RefSeq Version	XM_018094102.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpain-8-like (LOC100497284), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094102.1

ORF Insert Sequence:

ATGGAAGAGA CCCAAGGGGA CTGCTGGCTC CTGGCGGCCA TCGCATCTCT TACACTGGAT 
CCAGACCTTG TAGCCCAGGT GGTTCCAGAG AACCAAAGTT TCCAGAAGGG CTACGCTGGG 
ATCTTCCACT TCCAGTTCTG GCAGTACGGA GAGTGGGTGG ACGTGGTGGT GGATGACCGG 
CTGCCTATGA AGGATGGGAA CCTGGTGTTT GTACACTCGG CTGAAGGGGA CGAATTCTGG 
AGCGCTCTGC TAGAGAAGGC CTACGCAAAG TTGAATGGCT CCTACGAGGC TCTGACTGGC 
GGTTTGACCA TAGAGGGATT TGAAGACTTT ACCGGAGGTA TCGCCGAGGT GTATGAACTG 
AACGCGGCTC CGTCTGATTT ATTCCAGATC ATCCAAAAAG CCCTTAGGGC TGAATCACTG 
CTTGGCTGCT CTATTGACAT TTCTAATTCA AATGACACTG AAGCCGAGAC AAGCAGAAAG 
CTGGTTAAGG GGCACGCGTA TTCAGTGACG GGCGCCGAGG AGGTGTTATA CCGAGGGCGC 
CGGGAGAAGC TGATCCGAGT GAGAAATCCC TGGGGAGAAG TAGAATGGAC CGGACCTTGG 
AGTGATTATG CTCCAGAGTG GAATTATATT GATCCTAAGG TGAAGGCAGC TCTGGATAAA 
CAGTCTGACG ATGGAGAATT TTGGATGGCG TTTTCTGACT TTCTCCGAGA GTATTCCCGC 
CTGGAGATCT GTAACCTGTC CCCCGACACA CTCACCAGCA CCGAGCAGCA TAAATGGAAC 
ATAACCCTGT ACAATGGGAG CTGGGCACGG GGGTCTACTG CCGGCGGCTG CCCCAAATAC 
CCAGAAACAT TCTGGACAAA CCCACAGTTT CGCATTAAAC TGGATACGCC GGATTGGGGC 
CACAAGGGGT CGGATAATGA GCCCTGCTGT ACAGTCATTG TGGGGCTGAT GCAGAAGAAC 
CGCAGACGGA ACAGAAAGAT GGGGGAGGAT TTGCTTAGCA TCGGCTTCTC CATATTTAGG 
ATCCCTGATC AGCTTCGAGG CCACACTGAT AGCAACTTCA GCAGGGAATT CTTCCAAAGG 
TCTCGAACAG TGGCTCGATC TGACGCCTAT ATAAACATAC GGGAGGTCTC CCAGCGCTTC 
TATCTGCCAG TCGGGGATTA TCTCATCGTC CCAACCACCT TTGAGCCTTT CCAAAATGGC 
GACTTCTGCC TTCGAATCTT CTCAGAAAAG GAGGCCAAAT CTCTGGAAGT CGGAAATGTG 
GTGACAGTAA ATCCATATGA GCCTCAGATT TCTAAAAGGG CCACTGATGA ACTCAAGGAG 
ATGTTTTATG AGTTTGCCGG AGAAGTAAGA ACATTTTCCC CTAATCTGAC AGTACATTAT 
AAAGAAGAAC TTGATGCTAC AGATCTCCAA AGCATTCTCA ATGTGCTCCT ATCAATCCGT 
GATCGTTGCC CAGAAACGCC ACGTGCAACT GACAAGCACT TACACAAGAA ACGGAGTTGT 
TTTCAGGGAC TTTGGTGCCA CATTGGGCGC TCCTCTGAAA CGCGGCGCTC AAAGCGACTA 
GAAACACCCC CCGCGCCACA TCCCACAAGG CCGGACTTGA GATCGGAGAA ATTTACCCTT 
AGCACTTGCA GGGAGATGAT CAGCCTCCAG GATATGGATG GGACAGAAAC ACTGAACCTC 
CGGGAGTTCC GAGTTCTCTG GATGAAGATA CAGAAATACA TGACAATCTA TTTAAGGGCG 
GACTCGGATC GTTCTGGAAC CATAGACGCT CTTGAGCTGA GAACAGCTTT ACCACAAGCA 
GGTTTTACTC TGAATAATAA GATCCAGCAG CGCATTGTGA AGCGATACGC CTCTGAGGAC 
CTGGCTATTA ACTTAGATGC CTTTATTGCC TGTATGATGC GCCTGGAGAC CCTGTTCAAA 
ATGTTCGAGA TATTGGACAA GAGCAAGAGA GGGGTCGTTG AGCTGGATTT ACAAGAGTGG 
CTCTGTGCAA CACTGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100497284 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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