gramd1b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	gramd1b
Entrez Gene ID	100497114
Full Name	GRAM domain containing 1B
General protein information	Preferred Names GRAM domain containing 1B Names GRAM domain-containing protein 1B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031905706.1	XP_031761566.1	protein Aster-B isoform X2
XM_031905707.1	XP_031761567.1	protein Aster-B isoform X3
XM_012967085.2	XP_012822539.1	GRAM domain-containing protein 1B isoform X2
XM_031905705.1	XP_031761565.1	protein Aster-B isoform X1
XM_018095885.1	XP_017951374.1	GRAM domain-containing protein 1B isoform X1
XM_018095886.1	XP_017951375.1	GRAM domain-containing protein 1B isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GRAMD1B	NP_001178348.1
Gallus gallus (chicken)	GRAMD1B	XP_003642648.2
	GRAMD1A	XP_001108370.2
Canis lupus familiaris (dog)	GRAMD1B	XP_005619703.1
Rattus norvegicus (Norway rat)	Gramd1b	NP_001178545.1
Homo sapiens (human)	GRAMD1B	NP_001273492.1
Pan troglodytes (chimpanzee)	GRAMD1B	XP_508832.4
Mus musculus (house mouse)	Gramd1b	NP_766356.1
Danio rerio (zebrafish)	gramd1b	XP_002665458.3
Xenopus tropicalis (tropical clawed frog)	gramd1b	XP_002937578.2

Gene Ontology

The following gramd1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gramd1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa36776	XM_012967085.2 Latest version!	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa45779	XM_018095885.1 Latest version!	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa45780	XM_018095886.1 Latest version!	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa62120	XM_031905705.1 Latest version!	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa62121	XM_031905706.1 Latest version!	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa62122	XM_031905707.1 Latest version!	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gramd1b ( XM_012967085.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822539.1 Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa36776
Clone ID Related Accession (Same CDS sequence)	XM_012967085.2
Accession Version	XM_012967085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	GRAM domain-containing protein 1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGTCAATAGT TGAAACACTT TCCATGCCCT TTGAGAATTC CTTCCAGCAG 
CATCTAATGG ACACCATGGA GTGCACCACA GACTGGGAAA TGGACAGTTT TCAGGAAGAA 
GCGTTACTGT GGTACCTGGA CAGGTTAAGT TACATTCCAC AGGAGTGCGG GCTCCACAAC 
ACTTCTGAGT GTGAAGAAGT TCCAGCTGTA CTTAGTCCAA CATACAAACA AAGAAATGAA 
GATTTTCGAA AACTTTTTAA ACAGCTACCA GATTCTGAGC GTCTCATTGT AGATTATTCT 
TGTGCTTTGC AAAGGGACAT CCTTTTGCAA GGGCGGCTCT ACCTGTCTGA GAATTGGATC 
TGTTTCTACA GCAACATCTT CCGTTGGGAG ACATTGCTAA CAGTACGATT AAAAGATATC 
TGTTCGATGA CCAAGGAGAA GACTGCCAGA CTTATTCCAA ATGCCATCCA ACTCTGCACT 
GAAACTGAAA AGCACTTTTT TACATCATTT GGGGCTCGTG ATCGAACCTA CATGATGATG 
TTCCGGCTTT GGCAGAATGC TTTGCTGGAA AAGCCTCTGT GTCCAAAGGA GCTCTGGCAT 
TTTGTCCATC AGTGTTACGG GAATGAGCTA GGGCTTACAA GTGACGACGA AGACTATGTG 
CCTCCAGATG ATGACTTTAA TACAATGGGA TTCTGTGAAG AAATTCCTGT GGAAGAAGTG 
GAAGTAGTAA ACTACAATTC ATCAAAAAGC AGCAATGAGG TAAAACCAGA GACCAACTCA 
AACCTACAGG ACCCATCTTT AACTAGTAGC AATGTGACAT CCACTGCCAG CAGTGATGCC 
CCAGCATTTG ATGGGGGAAT GCTAGATGAG ATCGACAGCT CATTAGATAA GGAGATATTA 
ATTGCAAATC TTGAGGAACA GAAGGTGGTA CTTCTTCCTC CTGTGACTTC ACCATCCTTG 
GACTTTAATG ATAATGAAGA TCTCCCCACT GAGTTGAGCG ATTCCTCAGA TACCCATGAT 
GAAGGTGAAG TGCAGGCCTT TTATGAGGAT CTGAATGGTC GACAGTATAT CAATGAGGTA 
TTTAGTTTTA ATGTGGACAA GCTCTTCAGC CTTCTTTTCA CTGAATCTCA GTTCCAAAGA 
GACTTCATGG AACAGAGACG CTTCACGGAA GTAATATTTC ACCCCTGGAA GAAGGAAGAA 
AATGGCAACC AGACAAGAGT TATTCTTTAC ACCATTGCAC TTTCTAACCC ATTAGCACCC 
AAATCAGCCA CAGTCACAGA GAATCAGACA TTGTACAAGG CAAGCCAAGA GAGTGAGTGC 
TATGTGGTAG ATGCCGAAGT TCAGACTCAC GATATTCCTT ATCATGATTA CTTTTACACC 
ATCAATCGGT ACACATTGAC AAGAGTGGCC AGGAATAAGA GCAGACTCAG GATCTCAACA 
GAGTTACGTT ATCGGAAACA ACCATGGGGT CTTGTCAAAA CCTTCATAGA AAAAAACTTT 
TGGAGTGGAC TTGATGAGTA TTTTCATCAT TTAGAGAATG AACTGACCAA AGCAGAGACG 
GCATACTTAT CTGATCTACA TCAACAGTCC CCCAAAGTAA AAAGCAGCAA AATCACTTCC 
ACGCGCAGAA GGAAACGTCC CCATACTCAC CTGCGCATCC CACATCTACA GGAAGTCTTG 
AGCCCAGTTA CAACTCCCAC CGATGAAGAG AGGCAGCATC ATCACATCAG ACATGTTGCA 
GGTTCTACTC AAACGCGGCA TGTTCCAGAA GAGAAACCAA GTCATTTACA AAGCATTTCT 
AAACTTCTGC TGGTAATCAG CTGTGTTTTG GTGCTGCTAG TGATGCTCAA TGCAATGCTA 
TTTTATAAGC TGTGGGTGCT GGAATATAGT ACACAAGCAC TGACAACATG GCAAGGCCTC 
AGGCTTCAGG ACAGCCACCT GCCTCAATCA CAAGCTGAGT GGACACAGTT GTTAGAATCA 
CAACAGAAGC ACCATGACAC TGAAATGCAA AAATGGAAGC AAATCATTAA GTCTTCTGTG 
ATGCTGCTGG ACCAGATGAA GGATTCGCTA ATAAACCTTC AGAATGGAAT AATTTCCCGA 
GACTTTGTTT CAGAATCAGA AGAAAAACAG TACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822539.1
CDS	330..2468
Translation	MKEKSIVETLSMPFENSFQQHLMDTMECTTDWEMDSFQEEALLWYLDRLSYIPQECGLHNTSECEEVPAVLSPTYKQRNEDFRKLFKQLPDSERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFCEEIPVEEVEVVNYNSSKSSNEVKPETNSNLQDPSLTSSNVTSTASSDAPAFDGGMLDEIDSSLDKEILIANLEEQKVVLLPPVTSPSLDFNDNEDLPTELSDSSDTHDEGEVQAFYEDLNGRQYINEVFSFNVDKLFSLLFTESQFQRDFMEQRRFTEVIFHPWKKEENGNQTRVILYTIALSNPLAPKSATVTENQTLYKASQESECYVVDAEVQTHDIPYHDYFYTINRYTLTRVARNKSRLRISTELRYRKQPWGLVKTFIEKNFWSGLDEYFHHLENELTKAETAYLSDLHQQSPKVKSSKITSTRRRKRPHTHLRIPHLQEVLSPVTTPTDEERQHHHIRHVAGSTQTRHVPEEKPSHLQSISKLLLVISCVLVLLVMLNAMLFYKLWVLEYSTQALTTWQGLRLQDSHLPQSQAEWTQLLESQQKHHDTEMQKWKQIIKSSVMLLDQMKDSLINLQNGIISRDFVSESEEKQYQ

Target ORF information:

RefSeq Version	XM_012967085.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967085.2

ORF Insert Sequence:

ATGAAAGAGA AGTCAATAGT TGAAACACTT TCCATGCCCT TTGAGAATTC CTTCCAGCAG 
CATCTAATGG ACACCATGGA GTGCACCACA GACTGGGAAA TGGACAGTTT TCAGGAAGAA 
GCGTTACTGT GGTACCTGGA CAGGTTAAGT TACATTCCAC AGGAGTGCGG GCTCCACAAC 
ACTTCTGAGT GTGAAGAAGT TCCAGCTGTA CTTAGTCCAA CATACAAACA AAGAAATGAA 
GATTTTCGAA AACTTTTTAA ACAGCTACCA GATTCTGAGC GTCTCATTGT AGATTATTCT 
TGTGCTTTGC AAAGGGACAT CCTTTTGCAA GGGCGGCTCT ACCTGTCTGA GAATTGGATC 
TGTTTCTACA GCAACATCTT CCGTTGGGAG ACATTGCTAA CAGTACGATT AAAAGATATC 
TGTTCGATGA CCAAGGAGAA GACTGCCAGA CTTATTCCAA ATGCCATCCA ACTCTGCACT 
GAAACTGAAA AGCACTTTTT TACATCATTT GGGGCTCGTG ATCGAACCTA CATGATGATG 
TTCCGGCTTT GGCAGAATGC TTTGCTGGAA AAGCCTCTGT GTCCAAAGGA GCTCTGGCAT 
TTTGTCCATC AGTGTTACGG GAATGAGCTA GGGCTTACAA GTGACGACGA AGACTATGTG 
CCTCCAGATG ATGACTTTAA TACAATGGGA TTCTGTGAAG AAATTCCTGT GGAAGAAGTG 
GAAGTAGTAA ACTACAATTC ATCAAAAAGC AGCAATGAGG TAAAACCAGA GACCAACTCA 
AACCTACAGG ACCCATCTTT AACTAGTAGC AATGTGACAT CCACTGCCAG CAGTGATGCC 
CCAGCATTTG ATGGGGGAAT GCTAGATGAG ATCGACAGCT CATTAGATAA GGAGATATTA 
ATTGCAAATC TTGAGGAACA GAAGGTGGTA CTTCTTCCTC CTGTGACTTC ACCATCCTTG 
GACTTTAATG ATAATGAAGA TCTCCCCACT GAGTTGAGCG ATTCCTCAGA TACCCATGAT 
GAAGGTGAAG TGCAGGCCTT TTATGAGGAT CTGAATGGTC GACAGTATAT CAATGAGGTA 
TTTAGTTTTA ATGTGGACAA GCTCTTCAGC CTTCTTTTCA CTGAATCTCA GTTCCAAAGA 
GACTTCATGG AACAGAGACG CTTCACGGAA GTAATATTTC ACCCCTGGAA GAAGGAAGAA 
AATGGCAACC AGACAAGAGT TATTCTTTAC ACCATTGCAC TTTCTAACCC ATTAGCACCC 
AAATCAGCCA CAGTCACAGA GAATCAGACA TTGTACAAGG CAAGCCAAGA GAGTGAGTGC 
TATGTGGTAG ATGCCGAAGT TCAGACTCAC GATATTCCTT ATCATGATTA CTTTTACACC 
ATCAATCGGT ACACATTGAC AAGAGTGGCC AGGAATAAGA GCAGACTCAG GATCTCAACA 
GAGTTACGTT ATCGGAAACA ACCATGGGGT CTTGTCAAAA CCTTCATAGA AAAAAACTTT 
TGGAGTGGAC TTGATGAGTA TTTTCATCAT TTAGAGAATG AACTGACCAA AGCAGAGACG 
GCATACTTAT CTGATCTACA TCAACAGTCC CCCAAAGTAA AAAGCAGCAA AATCACTTCC 
ACGCGCAGAA GGAAACGTCC CCATACTCAC CTGCGCATCC CACATCTACA GGAAGTCTTG 
AGCCCAGTTA CAACTCCCAC CGATGAAGAG AGGCAGCATC ATCACATCAG ACATGTTGCA 
GGTTCTACTC AAACGCGGCA TGTTCCAGAA GAGAAACCAA GTCATTTACA AAGCATTTCT 
AAACTTCTGC TGGTAATCAG CTGTGTTTTG GTGCTGCTAG TGATGCTCAA TGCAATGCTA 
TTTTATAAGC TGTGGGTGCT GGAATATAGT ACACAAGCAC TGACAACATG GCAAGGCCTC 
AGGCTTCAGG ACAGCCACCT GCCTCAATCA CAAGCTGAGT GGACACAGTT GTTAGAATCA 
CAACAGAAGC ACCATGACAC TGAAATGCAA AAATGGAAGC AAATCATTAA GTCTTCTGTG 
ATGCTGCTGG ACCAGATGAA GGATTCGCTA ATAAACCTTC AGAATGGAAT AATTTCCCGA 
GACTTTGTTT CAGAATCAGA AGAAAAACAG TACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gramd1b ( XM_018095885.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951374.1 Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa45779
Clone ID Related Accession (Same CDS sequence)	XM_018095885.1
Accession Version	XM_018095885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	GRAM domain-containing protein 1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGCA GCACGGCAAG TAACTCAAAT CGAAGTTCTC CTGCTTGCTC CCCCATTCTG 
CGAAAGCGCT CCCGCTCACC AACACCCCAG CAAAGTGAGG GAGAAATCAT GGTGGAGAAG 
AGCTCAGATC ATTCTTCAGA CAAATCTCCT CCTACACCAG AACCTGGTGT GCAACGAAGT 
GGATCGTCTC AGTCTGGACG TAGCAGTGGC AAAAATTCAA AGAAAAGCCA GAGCTGGTAC 
AATGTACTTA GTCCAACATA CAAACAAAGA AATGAAGATT TTCGAAAACT TTTTAAACAG 
CTACCAGATT CTGAGCGTCT CATTGTAGAT TATTCTTGTG CTTTGCAAAG GGACATCCTT 
TTGCAAGGGC GGCTCTACCT GTCTGAGAAT TGGATCTGTT TCTACAGCAA CATCTTCCGT 
TGGGAGACAT TGCTAACAGT ACGATTAAAA GATATCTGTT CGATGACCAA GGAGAAGACT 
GCCAGACTTA TTCCAAATGC CATCCAACTC TGCACTGAAA CTGAAAAGCA CTTTTTTACA 
TCATTTGGGG CTCGTGATCG AACCTACATG ATGATGTTCC GGCTTTGGCA GAATGCTTTG 
CTGGAAAAGC CTCTGTGTCC AAAGGAGCTC TGGCATTTTG TCCATCAGTG TTACGGGAAT 
GAGCTAGGGC TTACAAGTGA CGACGAAGAC TATGTGCCTC CAGATGATGA CTTTAATACA 
ATGGGATTCT GTGAAGAAAT TCCTGTGGAA GAAGTGGAAG TAGTAAACTA CAATTCATCA 
AAAAGCAGCA ATGAGGTAAA ACCAGAGACC AACTCAAACC TACAGGACCC ATCTTTAACT 
AGTAGCAATG TGACATCCAC TGCCAGCAGT GATGCCCCAG CATTTGATGG GGGAATGCTA 
GATGAGATCG ACAGCTCATT AGATAAGGAG ATATTAATTG CAAATCTTGA GGAACAGAAG 
GTGGTACTTC TTCCTCCTGT GACTTCACCA TCCTTGGACT TTAATGATAA TGAAGATCTC 
CCCACTGAGT TGAGCGATTC CTCAGATACC CATGATGAAG GTGAAGTGCA GGCCTTTTAT 
GAGGATCTGA ATGGTCGACA GTATATCAAT GAGGTATTTA GTTTTAATGT GGACAAGCTC 
TTCAGCCTTC TTTTCACTGA ATCTCAGTTC CAAAGAGACT TCATGGAACA GAGACGCTTC 
ACGGAAGTAA TATTTCACCC CTGGAAGAAG GAAGAAAATG GCAACCAGAC AAGAGTTATT 
CTTTACACCA TTGCACTTTC TAACCCATTA GCACCCAAAT CAGCCACAGT CACAGAGAAT 
CAGACATTGT ACAAGGCAAG CCAAGAGAGT GAGTGCTATG TGGTAGATGC CGAAGTTCAG 
ACTCACGATA TTCCTTATCA TGATTACTTT TACACCATCA ATCGGTACAC ATTGACAAGA 
GTGGCCAGGA ATAAGAGCAG ACTCAGGATC TCAACAGAGT TACGTTATCG GAAACAACCA 
TGGGGTCTTG TCAAAACCTT CATAGAAAAA AACTTTTGGA GTGGACTTGA TGAGTATTTT 
CATCATTTAG AGAATGAACT GACCAAAGCA GAGACGGCAT ACTTATCTGA TCTACATCAA 
CAGTCCCCCA AAGTAAAAAG CAGCAAAATC ACTTCCACGC GCAGAAGGAA ACGTCCCCAT 
ACTCACCTGC GCATCCCACA TCTACAGGAA GTCTTGAGCC CAGTTACAAC TCCCACCGAT 
GAAGAGAGGC AGCATCATCA CATCAGACAT GTTGCAGGTT CTACTCAAAC GCGGCATGTT 
CCAGAAGAGA AACCAAGTCA TTTACAAAGC ATTTCTAAAC TTCTGCTGGT AATCAGCTGT 
GTTTTGGTGC TGCTAGTGAT GCTCAATGCA ATGCTATTTT ATAAGCTGTG GGTGCTGGAA 
TATAGTACAC AAGCACTGAC AACATGGCAA GGCCTCAGGC TTCAGGACAG CCACCTGCCT 
CAATCACAAG CTGAGTGGAC ACAGTTGTTA GAATCACAAC AGAAGCACCA TGACACTGAA 
ATGCAAAAAT GGAAGCAAAT CATTAAGTCT TCTGTGATGC TGCTGGACCA GATGAAGGAT 
TCGCTAATAA ACCTTCAGAA TGGAATAATT TCCCGAGACT TTGTTTCAGA ATCAGAAGAA 
AAACAGTACC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951374.1
CDS	608..2782
Translation	MACSTASNSNRSSPACSPILRKRSRSPTPQQSEGEIMVEKSSDHSSDKSPPTPEPGVQRSGSSQSGRSSGKNSKKSQSWYNVLSPTYKQRNEDFRKLFKQLPDSERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFCEEIPVEEVEVVNYNSSKSSNEVKPETNSNLQDPSLTSSNVTSTASSDAPAFDGGMLDEIDSSLDKEILIANLEEQKVVLLPPVTSPSLDFNDNEDLPTELSDSSDTHDEGEVQAFYEDLNGRQYINEVFSFNVDKLFSLLFTESQFQRDFMEQRRFTEVIFHPWKKEENGNQTRVILYTIALSNPLAPKSATVTENQTLYKASQESECYVVDAEVQTHDIPYHDYFYTINRYTLTRVARNKSRLRISTELRYRKQPWGLVKTFIEKNFWSGLDEYFHHLENELTKAETAYLSDLHQQSPKVKSSKITSTRRRKRPHTHLRIPHLQEVLSPVTTPTDEERQHHHIRHVAGSTQTRHVPEEKPSHLQSISKLLLVISCVLVLLVMLNAMLFYKLWVLEYSTQALTTWQGLRLQDSHLPQSQAEWTQLLESQQKHHDTEMQKWKQIIKSSVMLLDQMKDSLINLQNGIISRDFVSESEEKQYQ

Target ORF information:

RefSeq Version	XM_018095885.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095885.1

ORF Insert Sequence:

ATGGCATGCA GCACGGCAAG TAACTCAAAT CGAAGTTCTC CTGCTTGCTC CCCCATTCTG 
CGAAAGCGCT CCCGCTCACC AACACCCCAG CAAAGTGAGG GAGAAATCAT GGTGGAGAAG 
AGCTCAGATC ATTCTTCAGA CAAATCTCCT CCTACACCAG AACCTGGTGT GCAACGAAGT 
GGATCGTCTC AGTCTGGACG TAGCAGTGGC AAAAATTCAA AGAAAAGCCA GAGCTGGTAC 
AATGTACTTA GTCCAACATA CAAACAAAGA AATGAAGATT TTCGAAAACT TTTTAAACAG 
CTACCAGATT CTGAGCGTCT CATTGTAGAT TATTCTTGTG CTTTGCAAAG GGACATCCTT 
TTGCAAGGGC GGCTCTACCT GTCTGAGAAT TGGATCTGTT TCTACAGCAA CATCTTCCGT 
TGGGAGACAT TGCTAACAGT ACGATTAAAA GATATCTGTT CGATGACCAA GGAGAAGACT 
GCCAGACTTA TTCCAAATGC CATCCAACTC TGCACTGAAA CTGAAAAGCA CTTTTTTACA 
TCATTTGGGG CTCGTGATCG AACCTACATG ATGATGTTCC GGCTTTGGCA GAATGCTTTG 
CTGGAAAAGC CTCTGTGTCC AAAGGAGCTC TGGCATTTTG TCCATCAGTG TTACGGGAAT 
GAGCTAGGGC TTACAAGTGA CGACGAAGAC TATGTGCCTC CAGATGATGA CTTTAATACA 
ATGGGATTCT GTGAAGAAAT TCCTGTGGAA GAAGTGGAAG TAGTAAACTA CAATTCATCA 
AAAAGCAGCA ATGAGGTAAA ACCAGAGACC AACTCAAACC TACAGGACCC ATCTTTAACT 
AGTAGCAATG TGACATCCAC TGCCAGCAGT GATGCCCCAG CATTTGATGG GGGAATGCTA 
GATGAGATCG ACAGCTCATT AGATAAGGAG ATATTAATTG CAAATCTTGA GGAACAGAAG 
GTGGTACTTC TTCCTCCTGT GACTTCACCA TCCTTGGACT TTAATGATAA TGAAGATCTC 
CCCACTGAGT TGAGCGATTC CTCAGATACC CATGATGAAG GTGAAGTGCA GGCCTTTTAT 
GAGGATCTGA ATGGTCGACA GTATATCAAT GAGGTATTTA GTTTTAATGT GGACAAGCTC 
TTCAGCCTTC TTTTCACTGA ATCTCAGTTC CAAAGAGACT TCATGGAACA GAGACGCTTC 
ACGGAAGTAA TATTTCACCC CTGGAAGAAG GAAGAAAATG GCAACCAGAC AAGAGTTATT 
CTTTACACCA TTGCACTTTC TAACCCATTA GCACCCAAAT CAGCCACAGT CACAGAGAAT 
CAGACATTGT ACAAGGCAAG CCAAGAGAGT GAGTGCTATG TGGTAGATGC CGAAGTTCAG 
ACTCACGATA TTCCTTATCA TGATTACTTT TACACCATCA ATCGGTACAC ATTGACAAGA 
GTGGCCAGGA ATAAGAGCAG ACTCAGGATC TCAACAGAGT TACGTTATCG GAAACAACCA 
TGGGGTCTTG TCAAAACCTT CATAGAAAAA AACTTTTGGA GTGGACTTGA TGAGTATTTT 
CATCATTTAG AGAATGAACT GACCAAAGCA GAGACGGCAT ACTTATCTGA TCTACATCAA 
CAGTCCCCCA AAGTAAAAAG CAGCAAAATC ACTTCCACGC GCAGAAGGAA ACGTCCCCAT 
ACTCACCTGC GCATCCCACA TCTACAGGAA GTCTTGAGCC CAGTTACAAC TCCCACCGAT 
GAAGAGAGGC AGCATCATCA CATCAGACAT GTTGCAGGTT CTACTCAAAC GCGGCATGTT 
CCAGAAGAGA AACCAAGTCA TTTACAAAGC ATTTCTAAAC TTCTGCTGGT AATCAGCTGT 
GTTTTGGTGC TGCTAGTGAT GCTCAATGCA ATGCTATTTT ATAAGCTGTG GGTGCTGGAA 
TATAGTACAC AAGCACTGAC AACATGGCAA GGCCTCAGGC TTCAGGACAG CCACCTGCCT 
CAATCACAAG CTGAGTGGAC ACAGTTGTTA GAATCACAAC AGAAGCACCA TGACACTGAA 
ATGCAAAAAT GGAAGCAAAT CATTAAGTCT TCTGTGATGC TGCTGGACCA GATGAAGGAT 
TCGCTAATAA ACCTTCAGAA TGGAATAATT TCCCGAGACT TTGTTTCAGA ATCAGAAGAA 
AAACAGTACC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gramd1b ( XM_018095886.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951375.1 Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa45780
Clone ID Related Accession (Same CDS sequence)	XM_018095886.1
Accession Version	XM_018095886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	GRAM domain-containing protein 1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAGA AGAGCTCAGA TCATTCTTCA GACAAATCTC CTCCTACACC AGAACCTGGT 
GTGCAACGAA GTGGATCGTC TCAGTCTGGA CGTAGCAGTG GCAAAAATTC AAAGAAAAGC 
CAGAGCTGGT ACAATGTACT TAGTCCAACA TACAAACAAA GAAATGAAGA TTTTCGAAAA 
CTTTTTAAAC AGCTACCAGA TTCTGAGCGT CTCATTGTAG ATTATTCTTG TGCTTTGCAA 
AGGGACATCC TTTTGCAAGG GCGGCTCTAC CTGTCTGAGA ATTGGATCTG TTTCTACAGC 
AACATCTTCC GTTGGGAGAC ATTGCTAACA GTACGATTAA AAGATATCTG TTCGATGACC 
AAGGAGAAGA CTGCCAGACT TATTCCAAAT GCCATCCAAC TCTGCACTGA AACTGAAAAG 
CACTTTTTTA CATCATTTGG GGCTCGTGAT CGAACCTACA TGATGATGTT CCGGCTTTGG 
CAGAATGCTT TGCTGGAAAA GCCTCTGTGT CCAAAGGAGC TCTGGCATTT TGTCCATCAG 
TGTTACGGGA ATGAGCTAGG GCTTACAAGT GACGACGAAG ACTATGTGCC TCCAGATGAT 
GACTTTAATA CAATGGGATT CTGTGAAGAA ATTCCTGTGG AAGAAGTGGA AGTAGTAAAC 
TACAATTCAT CAAAAAGCAG CAATGAGGTA AAACCAGAGA CCAACTCAAA CCTACAGGAC 
CCATCTTTAA CTAGTAGCAA TGTGACATCC ACTGCCAGCA GTGATGCCCC AGCATTTGAT 
GGGGGAATGC TAGATGAGAT CGACAGCTCA TTAGATAAGG AGATATTAAT TGCAAATCTT 
GAGGAACAGA AGGTGGTACT TCTTCCTCCT GTGACTTCAC CATCCTTGGA CTTTAATGAT 
AATGAAGATC TCCCCACTGA GTTGAGCGAT TCCTCAGATA CCCATGATGA AGGTGAAGTG 
CAGGCCTTTT ATGAGGATCT GAATGGTCGA CAGTATATCA ATGAGGTATT TAGTTTTAAT 
GTGGACAAGC TCTTCAGCCT TCTTTTCACT GAATCTCAGT TCCAAAGAGA CTTCATGGAA 
CAGAGACGCT TCACGGAAGT AATATTTCAC CCCTGGAAGA AGGAAGAAAA TGGCAACCAG 
ACAAGAGTTA TTCTTTACAC CATTGCACTT TCTAACCCAT TAGCACCCAA ATCAGCCACA 
GTCACAGAGA ATCAGACATT GTACAAGGCA AGCCAAGAGA GTGAGTGCTA TGTGGTAGAT 
GCCGAAGTTC AGACTCACGA TATTCCTTAT CATGATTACT TTTACACCAT CAATCGGTAC 
ACATTGACAA GAGTGGCCAG GAATAAGAGC AGACTCAGGA TCTCAACAGA GTTACGTTAT 
CGGAAACAAC CATGGGGTCT TGTCAAAACC TTCATAGAAA AAAACTTTTG GAGTGGACTT 
GATGAGTATT TTCATCATTT AGAGAATGAA CTGACCAAAG CAGAGACGGC ATACTTATCT 
GATCTACATC AACAGTCCCC CAAAGTAAAA AGCAGCAAAA TCACTTCCAC GCGCAGAAGG 
AAACGTCCCC ATACTCACCT GCGCATCCCA CATCTACAGG AAGTCTTGAG CCCAGTTACA 
ACTCCCACCG ATGAAGAGAG GCAGCATCAT CACATCAGAC ATGTTGCAGG TTCTACTCAA 
ACGCGGCATG TTCCAGAAGA GAAACCAAGT CATTTACAAA GCATTTCTAA ACTTCTGCTG 
GTAATCAGCT GTGTTTTGGT GCTGCTAGTG ATGCTCAATG CAATGCTATT TTATAAGCTG 
TGGGTGCTGG AATATAGTAC ACAAGCACTG ACAACATGGC AAGGCCTCAG GCTTCAGGAC 
AGCCACCTGC CTCAATCACA AGCTGAGTGG ACACAGTTGT TAGAATCACA ACAGAAGCAC 
CATGACACTG AAATGCAAAA ATGGAAGCAA ATCATTAAGT CTTCTGTGAT GCTGCTGGAC 
CAGATGAAGG ATTCGCTAAT AAACCTTCAG AATGGAATAA TTTCCCGAGA CTTTGTTTCA 
GAATCAGAAG AAAAACAGTA CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951375.1
CDS	271..2337
Translation	MVEKSSDHSSDKSPPTPEPGVQRSGSSQSGRSSGKNSKKSQSWYNVLSPTYKQRNEDFRKLFKQLPDSERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFCEEIPVEEVEVVNYNSSKSSNEVKPETNSNLQDPSLTSSNVTSTASSDAPAFDGGMLDEIDSSLDKEILIANLEEQKVVLLPPVTSPSLDFNDNEDLPTELSDSSDTHDEGEVQAFYEDLNGRQYINEVFSFNVDKLFSLLFTESQFQRDFMEQRRFTEVIFHPWKKEENGNQTRVILYTIALSNPLAPKSATVTENQTLYKASQESECYVVDAEVQTHDIPYHDYFYTINRYTLTRVARNKSRLRISTELRYRKQPWGLVKTFIEKNFWSGLDEYFHHLENELTKAETAYLSDLHQQSPKVKSSKITSTRRRKRPHTHLRIPHLQEVLSPVTTPTDEERQHHHIRHVAGSTQTRHVPEEKPSHLQSISKLLLVISCVLVLLVMLNAMLFYKLWVLEYSTQALTTWQGLRLQDSHLPQSQAEWTQLLESQQKHHDTEMQKWKQIIKSSVMLLDQMKDSLINLQNGIISRDFVSESEEKQYQ

Target ORF information:

RefSeq Version	XM_018095886.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095886.1

ORF Insert Sequence:

ATGGTGGAGA AGAGCTCAGA TCATTCTTCA GACAAATCTC CTCCTACACC AGAACCTGGT 
GTGCAACGAA GTGGATCGTC TCAGTCTGGA CGTAGCAGTG GCAAAAATTC AAAGAAAAGC 
CAGAGCTGGT ACAATGTACT TAGTCCAACA TACAAACAAA GAAATGAAGA TTTTCGAAAA 
CTTTTTAAAC AGCTACCAGA TTCTGAGCGT CTCATTGTAG ATTATTCTTG TGCTTTGCAA 
AGGGACATCC TTTTGCAAGG GCGGCTCTAC CTGTCTGAGA ATTGGATCTG TTTCTACAGC 
AACATCTTCC GTTGGGAGAC ATTGCTAACA GTACGATTAA AAGATATCTG TTCGATGACC 
AAGGAGAAGA CTGCCAGACT TATTCCAAAT GCCATCCAAC TCTGCACTGA AACTGAAAAG 
CACTTTTTTA CATCATTTGG GGCTCGTGAT CGAACCTACA TGATGATGTT CCGGCTTTGG 
CAGAATGCTT TGCTGGAAAA GCCTCTGTGT CCAAAGGAGC TCTGGCATTT TGTCCATCAG 
TGTTACGGGA ATGAGCTAGG GCTTACAAGT GACGACGAAG ACTATGTGCC TCCAGATGAT 
GACTTTAATA CAATGGGATT CTGTGAAGAA ATTCCTGTGG AAGAAGTGGA AGTAGTAAAC 
TACAATTCAT CAAAAAGCAG CAATGAGGTA AAACCAGAGA CCAACTCAAA CCTACAGGAC 
CCATCTTTAA CTAGTAGCAA TGTGACATCC ACTGCCAGCA GTGATGCCCC AGCATTTGAT 
GGGGGAATGC TAGATGAGAT CGACAGCTCA TTAGATAAGG AGATATTAAT TGCAAATCTT 
GAGGAACAGA AGGTGGTACT TCTTCCTCCT GTGACTTCAC CATCCTTGGA CTTTAATGAT 
AATGAAGATC TCCCCACTGA GTTGAGCGAT TCCTCAGATA CCCATGATGA AGGTGAAGTG 
CAGGCCTTTT ATGAGGATCT GAATGGTCGA CAGTATATCA ATGAGGTATT TAGTTTTAAT 
GTGGACAAGC TCTTCAGCCT TCTTTTCACT GAATCTCAGT TCCAAAGAGA CTTCATGGAA 
CAGAGACGCT TCACGGAAGT AATATTTCAC CCCTGGAAGA AGGAAGAAAA TGGCAACCAG 
ACAAGAGTTA TTCTTTACAC CATTGCACTT TCTAACCCAT TAGCACCCAA ATCAGCCACA 
GTCACAGAGA ATCAGACATT GTACAAGGCA AGCCAAGAGA GTGAGTGCTA TGTGGTAGAT 
GCCGAAGTTC AGACTCACGA TATTCCTTAT CATGATTACT TTTACACCAT CAATCGGTAC 
ACATTGACAA GAGTGGCCAG GAATAAGAGC AGACTCAGGA TCTCAACAGA GTTACGTTAT 
CGGAAACAAC CATGGGGTCT TGTCAAAACC TTCATAGAAA AAAACTTTTG GAGTGGACTT 
GATGAGTATT TTCATCATTT AGAGAATGAA CTGACCAAAG CAGAGACGGC ATACTTATCT 
GATCTACATC AACAGTCCCC CAAAGTAAAA AGCAGCAAAA TCACTTCCAC GCGCAGAAGG 
AAACGTCCCC ATACTCACCT GCGCATCCCA CATCTACAGG AAGTCTTGAG CCCAGTTACA 
ACTCCCACCG ATGAAGAGAG GCAGCATCAT CACATCAGAC ATGTTGCAGG TTCTACTCAA 
ACGCGGCATG TTCCAGAAGA GAAACCAAGT CATTTACAAA GCATTTCTAA ACTTCTGCTG 
GTAATCAGCT GTGTTTTGGT GCTGCTAGTG ATGCTCAATG CAATGCTATT TTATAAGCTG 
TGGGTGCTGG AATATAGTAC ACAAGCACTG ACAACATGGC AAGGCCTCAG GCTTCAGGAC 
AGCCACCTGC CTCAATCACA AGCTGAGTGG ACACAGTTGT TAGAATCACA ACAGAAGCAC 
CATGACACTG AAATGCAAAA ATGGAAGCAA ATCATTAAGT CTTCTGTGAT GCTGCTGGAC 
CAGATGAAGG ATTCGCTAAT AAACCTTCAG AATGGAATAA TTTCCCGAGA CTTTGTTTCA 
GAATCAGAAG AAAAACAGTA CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gramd1b ( XM_031905705.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761565.1 Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa62120
Clone ID Related Accession (Same CDS sequence)	XM_031905705.1
Accession Version	XM_031905705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Aster-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGTCAATAGT TGAAACACTT TCCATGCCCT TTGAGAATTC CTTCCAGCAG 
CATCTAATGG ACACCATGGA GTGCACCACA GACTGGGAAA TGGACAGTTT TCAGGAAGAA 
GCGTTACTGT GGTACCTGGA CAGGTTAAGT TACATTCCAC AGGAGTGCGG GCTCCACAAC 
ACTTCTGAGT GTGAAGAAGT TCCAGCTGTA CTTAGTCCAA CATACAAACA AAGAAATGAA 
GATTTTCGAA AACTTTTTAA ACAGCTACCA GATTCTGAGC GTCTCATTGT AGATTATTCT 
TGTGCTTTGC AAAGGGACAT CCTTTTGCAA GGGCGGCTCT ACCTGTCTGA GAATTGGATC 
TGTTTCTACA GCAACATCTT CCGTTGGGAG ACATTGCTAA CAGTACGATT AAAAGATATC 
TGTTCGATGA CCAAGGAGAA GACTGCCAGA CTTATTCCAA ATGCCATCCA ACTCTGCACT 
GAAACTGAAA AGCACTTTTT TACATCATTT GGGGCTCGTG ATCGAACCTA CATGATGATG 
TTCCGGCTTT GGCAGAATGC TTTGCTGGAA AAGCCTCTGT GTCCAAAGGA GCTCTGGCAT 
TTTGTCCATC AGTGTTACGG GAATGAGCTA GGGCTTACAA GTGATGACGA AGACTATGTG 
CCTCCAGATG ATGACTTTAA TACAATGGGA TTCTGTGAAG AAATTCCTGT GGAAGAAGTG 
GAAGTAGTAA ACTACAATTC ATCAAAAAGC AGCAATGAGG TAAAACCAGA GACCAACTCA 
AACCTACAGG ACCCATCTTT AACTAGTAGC AATGTGACAT CCACTGCCAG CAGTGATGCC 
CCAGCATTTG ATGGGGGAAT GCTAGATGAG ATCGACAGCT CATTAGATAA GGAGATATTA 
ATTGCAAATC TTGAGGAACA GAAGGTGGTA CTTCTTCCTC CTGTGACTTC ACCATCCTTG 
GACTTTAATG ATAATGAAGA TCTCCCCACT GAGTTGAGCG ATTCCTCAGA TACCCATGAT 
GAAGGTGAAG TGCAGGCCTT TTATGAGGAT CTGAATGGTC GACAGTATAT CAATGAGGTA 
TTTAGTTTTA ATGTGGACAA GCTCTTCAGC CTTCTTTTCA CTGAATCTCA GTTCCAAAGA 
GACTTCATGG AACAGAGACG CTTCACGGAA GTAATATTTC ACCCCTGGAA GAAGGAAGAA 
AATGGCAACC AGACAAGAGT TATTCTTTAC ACCATTGCAC TTTCTAACCC ATTAGCACCC 
AAATCAGCCA CAGTCACAGA GAATCAGACA TTGTACAAGG CAAGCCAAGA GAGTGAGTGC 
TATGTGGTAG ATGCCGAAGT TCAGACTCAC GATATTCCTT ATCATGATTA CTTTTACACC 
ATCAATCGGT ACACATTGAC AAGAGTGGCC AGGAATAAGA GCAGACTCAG GATCTCAACA 
GAGTTACGTT ATCGGAAACA ACCATGGGGT CTTGTCAAAA CCTTCATAGA AAAAAACTTT 
TGGAGTGGAC TTGATGAGTA TTTTCATCAT TTAGAGAATG AACTGACCAA AGCAGAGACG 
GCATACTTAT CTGATCTACA TCAACAGTCC CCCAAAGTAA AAAGCAGCAA AATCACTTCC 
ACGCGCAGAA GGAAACGTCC CCATACTCAC CTGCGCATCC CACATCTACA GGAAGTCTTG 
AGCCCAGTTA CAACTCCCAC CGATGAAGAG AGGCAGCATC ATCACATCAG ACATGTTGCA 
GGTTCTACTC AAACGCGGCA TGTTCCAGAA GAGAAACCAA GTCATTTACA AAGCATTTCT 
AAACTTCTGC TGGTAATCAG CTGTGTTTTG GTGCTGCTAG TGATGCTCAA TGCAATGCTA 
TTTTATAAGC TGTGGGTGCT GGAATATAGT ACACAAGCAC TGACAACATG GCAAGGCCTC 
AGGCTTCAGG ACAGCCACCT GCCTCAATCA CAAGCTGAGT GGACACAGTT GTTAGAATCA 
CAACAGAAGC ACCATGACAC TGAAATGCAA AAATGGAAGC AAATCATTAA GTCTTCTGTG 
ATGCTGCTGG ACCAGATGAA GGATTCGCTA ATAAACCTTC AGAATGGAAT AATTTCCCGA 
GACTTTGTTT CAGAATCAGA AGAAAAACAG TACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761565.1
CDS	332..2470
Translation	MKEKSIVETLSMPFENSFQQHLMDTMECTTDWEMDSFQEEALLWYLDRLSYIPQECGLHNTSECEEVPAVLSPTYKQRNEDFRKLFKQLPDSERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFCEEIPVEEVEVVNYNSSKSSNEVKPETNSNLQDPSLTSSNVTSTASSDAPAFDGGMLDEIDSSLDKEILIANLEEQKVVLLPPVTSPSLDFNDNEDLPTELSDSSDTHDEGEVQAFYEDLNGRQYINEVFSFNVDKLFSLLFTESQFQRDFMEQRRFTEVIFHPWKKEENGNQTRVILYTIALSNPLAPKSATVTENQTLYKASQESECYVVDAEVQTHDIPYHDYFYTINRYTLTRVARNKSRLRISTELRYRKQPWGLVKTFIEKNFWSGLDEYFHHLENELTKAETAYLSDLHQQSPKVKSSKITSTRRRKRPHTHLRIPHLQEVLSPVTTPTDEERQHHHIRHVAGSTQTRHVPEEKPSHLQSISKLLLVISCVLVLLVMLNAMLFYKLWVLEYSTQALTTWQGLRLQDSHLPQSQAEWTQLLESQQKHHDTEMQKWKQIIKSSVMLLDQMKDSLINLQNGIISRDFVSESEEKQYQ

Target ORF information:

RefSeq Version	XM_031905705.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905705.1

ORF Insert Sequence:

ATGAAAGAGA AGTCAATAGT TGAAACACTT TCCATGCCCT TTGAGAATTC CTTCCAGCAG 
CATCTAATGG ACACCATGGA GTGCACCACA GACTGGGAAA TGGACAGTTT TCAGGAAGAA 
GCGTTACTGT GGTACCTGGA CAGGTTAAGT TACATTCCAC AGGAGTGCGG GCTCCACAAC 
ACTTCTGAGT GTGAAGAAGT TCCAGCTGTA CTTAGTCCAA CATACAAACA AAGAAATGAA 
GATTTTCGAA AACTTTTTAA ACAGCTACCA GATTCTGAGC GTCTCATTGT AGATTATTCT 
TGTGCTTTGC AAAGGGACAT CCTTTTGCAA GGGCGGCTCT ACCTGTCTGA GAATTGGATC 
TGTTTCTACA GCAACATCTT CCGTTGGGAG ACATTGCTAA CAGTACGATT AAAAGATATC 
TGTTCGATGA CCAAGGAGAA GACTGCCAGA CTTATTCCAA ATGCCATCCA ACTCTGCACT 
GAAACTGAAA AGCACTTTTT TACATCATTT GGGGCTCGTG ATCGAACCTA CATGATGATG 
TTCCGGCTTT GGCAGAATGC TTTGCTGGAA AAGCCTCTGT GTCCAAAGGA GCTCTGGCAT 
TTTGTCCATC AGTGTTACGG GAATGAGCTA GGGCTTACAA GTGATGACGA AGACTATGTG 
CCTCCAGATG ATGACTTTAA TACAATGGGA TTCTGTGAAG AAATTCCTGT GGAAGAAGTG 
GAAGTAGTAA ACTACAATTC ATCAAAAAGC AGCAATGAGG TAAAACCAGA GACCAACTCA 
AACCTACAGG ACCCATCTTT AACTAGTAGC AATGTGACAT CCACTGCCAG CAGTGATGCC 
CCAGCATTTG ATGGGGGAAT GCTAGATGAG ATCGACAGCT CATTAGATAA GGAGATATTA 
ATTGCAAATC TTGAGGAACA GAAGGTGGTA CTTCTTCCTC CTGTGACTTC ACCATCCTTG 
GACTTTAATG ATAATGAAGA TCTCCCCACT GAGTTGAGCG ATTCCTCAGA TACCCATGAT 
GAAGGTGAAG TGCAGGCCTT TTATGAGGAT CTGAATGGTC GACAGTATAT CAATGAGGTA 
TTTAGTTTTA ATGTGGACAA GCTCTTCAGC CTTCTTTTCA CTGAATCTCA GTTCCAAAGA 
GACTTCATGG AACAGAGACG CTTCACGGAA GTAATATTTC ACCCCTGGAA GAAGGAAGAA 
AATGGCAACC AGACAAGAGT TATTCTTTAC ACCATTGCAC TTTCTAACCC ATTAGCACCC 
AAATCAGCCA CAGTCACAGA GAATCAGACA TTGTACAAGG CAAGCCAAGA GAGTGAGTGC 
TATGTGGTAG ATGCCGAAGT TCAGACTCAC GATATTCCTT ATCATGATTA CTTTTACACC 
ATCAATCGGT ACACATTGAC AAGAGTGGCC AGGAATAAGA GCAGACTCAG GATCTCAACA 
GAGTTACGTT ATCGGAAACA ACCATGGGGT CTTGTCAAAA CCTTCATAGA AAAAAACTTT 
TGGAGTGGAC TTGATGAGTA TTTTCATCAT TTAGAGAATG AACTGACCAA AGCAGAGACG 
GCATACTTAT CTGATCTACA TCAACAGTCC CCCAAAGTAA AAAGCAGCAA AATCACTTCC 
ACGCGCAGAA GGAAACGTCC CCATACTCAC CTGCGCATCC CACATCTACA GGAAGTCTTG 
AGCCCAGTTA CAACTCCCAC CGATGAAGAG AGGCAGCATC ATCACATCAG ACATGTTGCA 
GGTTCTACTC AAACGCGGCA TGTTCCAGAA GAGAAACCAA GTCATTTACA AAGCATTTCT 
AAACTTCTGC TGGTAATCAG CTGTGTTTTG GTGCTGCTAG TGATGCTCAA TGCAATGCTA 
TTTTATAAGC TGTGGGTGCT GGAATATAGT ACACAAGCAC TGACAACATG GCAAGGCCTC 
AGGCTTCAGG ACAGCCACCT GCCTCAATCA CAAGCTGAGT GGACACAGTT GTTAGAATCA 
CAACAGAAGC ACCATGACAC TGAAATGCAA AAATGGAAGC AAATCATTAA GTCTTCTGTG 
ATGCTGCTGG ACCAGATGAA GGATTCGCTA ATAAACCTTC AGAATGGAAT AATTTCCCGA 
GACTTTGTTT CAGAATCAGA AGAAAAACAG TACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gramd1b ( XM_031905706.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761566.1 Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa62121
Clone ID Related Accession (Same CDS sequence)	XM_031905706.1
Accession Version	XM_031905706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Aster-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAGA AGAGCTCAGA TCATTCTTCA GACAAATCTC CTCCTACACC AGAACCTGGT 
GTGCAACGAA GTGGATCGTC TCAGTCTGGA CGTAGCAGTG GCAAAAATTC AAAGTATGAC 
AGACTTAACC TCATCAAAAA AAGCCAGAGC TGGTACAATG TACTTAGTCC AACATACAAA 
CAAAGAAATG AAGATTTTCG AAAACTTTTT AAACAGCTAC CAGATTCTGA GCGTCTCATT 
GTAGATTATT CTTGTGCTTT GCAAAGGGAC ATCCTTTTGC AAGGGCGGCT CTACCTGTCT 
GAGAATTGGA TCTGTTTCTA CAGCAACATC TTCCGTTGGG AGACATTGCT AACAGTACGA 
TTAAAAGATA TCTGTTCGAT GACCAAGGAG AAGACTGCCA GACTTATTCC AAATGCCATC 
CAACTCTGCA CTGAAACTGA AAAGCACTTT TTTACATCAT TTGGGGCTCG TGATCGAACC 
TACATGATGA TGTTCCGGCT TTGGCAGAAT GCTTTGCTGG AAAAGCCTCT GTGTCCAAAG 
GAGCTCTGGC ATTTTGTCCA TCAGTGTTAC GGGAATGAGC TAGGGCTTAC AAGTGATGAC 
GAAGACTATG TGCCTCCAGA TGATGACTTT AATACAATGG GATTCTGTGA AGAAATTCCT 
GTGGAAGAAG TGGAAGTAGT AAACTACAAT TCATCAAAAA GCAGCAATGA GGTAAAACCA 
GAGACCAACT CAAACCTACA GGACCCATCT TTAACTAGTA GCAATGTGAC ATCCACTGCC 
AGCAGTGATG CCCCAGCATT TGATGGGGGA ATGCTAGATG AGATCGACAG CTCATTAGAT 
AAGGAGATAT TAATTGCAAA TCTTGAGGAA CAGAAGGTGG TACTTCTTCC TCCTGTGACT 
TCACCATCCT TGGACTTTAA TGATAATGAA GATCTCCCCA CTGAGTTGAG CGATTCCTCA 
GATACCCATG ATGAAGGTGA AGTGCAGGCC TTTTATGAGG ATCTGAATGG TCGACAGTAT 
ATCAATGAGG TATTTAGTTT TAATGTGGAC AAGCTCTTCA GCCTTCTTTT CACTGAATCT 
CAGTTCCAAA GAGACTTCAT GGAACAGAGA CGCTTCACGG AAGTAATATT TCACCCCTGG 
AAGAAGGAAG AAAATGGCAA CCAGACAAGA GTTATTCTTT ACACCATTGC ACTTTCTAAC 
CCATTAGCAC CCAAATCAGC CACAGTCACA GAGAATCAGA CATTGTACAA GGCAAGCCAA 
GAGAGTGAGT GCTATGTGGT AGATGCCGAA GTTCAGACTC ACGATATTCC TTATCATGAT 
TACTTTTACA CCATCAATCG GTACACATTG ACAAGAGTGG CCAGGAATAA GAGCAGACTC 
AGGATCTCAA CAGAGTTACG TTATCGGAAA CAACCATGGG GTCTTGTCAA AACCTTCATA 
GAAAAAAACT TTTGGAGTGG ACTTGATGAG TATTTTCATC ATTTAGAGAA TGAACTGACC 
AAAGCAGAGA CGGCATACTT ATCTGATCTA CATCAACAGT CCCCCAAAGT AAAAAGCAGC 
AAAATCACTT CCACGCGCAG AAGGAAACGT CCCCATACTC ACCTGCGCAT CCCACATCTA 
CAGGAAGTCT TGAGCCCAGT TACAACTCCC ACCGATGAAG AGAGGCAGCA TCATCACATC 
AGACATGTTG CAGGTTCTAC TCAAACGCGG CATGTTCCAG AAGAGAAACC AAGTCATTTA 
CAAAGCATTT CTAAACTTCT GCTGGTAATC AGCTGTGTTT TGGTGCTGCT AGTGATGCTC 
AATGCAATGC TATTTTATAA GCTGTGGGTG CTGGAATATA GTACACAAGC ACTGACAACA 
TGGCAAGGCC TCAGGCTTCA GGACAGCCAC CTGCCTCAAT CACAAGCTGA GTGGACACAG 
TTGTTAGAAT CACAACAGAA GCACCATGAC ACTGAAATGC AAAAATGGAA GCAAATCATT 
AAGTCTTCTG TGATGCTGCT GGACCAGATG AAGGATTCGC TAATAAACCT TCAGAATGGA 
ATAATTTCCC GAGACTTTGT TTCAGAATCA GAAGAAAAAC AGTACCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761566.1
CDS	175..2265
Translation	MVEKSSDHSSDKSPPTPEPGVQRSGSSQSGRSSGKNSKYDRLNLIKKSQSWYNVLSPTYKQRNEDFRKLFKQLPDSERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFCEEIPVEEVEVVNYNSSKSSNEVKPETNSNLQDPSLTSSNVTSTASSDAPAFDGGMLDEIDSSLDKEILIANLEEQKVVLLPPVTSPSLDFNDNEDLPTELSDSSDTHDEGEVQAFYEDLNGRQYINEVFSFNVDKLFSLLFTESQFQRDFMEQRRFTEVIFHPWKKEENGNQTRVILYTIALSNPLAPKSATVTENQTLYKASQESECYVVDAEVQTHDIPYHDYFYTINRYTLTRVARNKSRLRISTELRYRKQPWGLVKTFIEKNFWSGLDEYFHHLENELTKAETAYLSDLHQQSPKVKSSKITSTRRRKRPHTHLRIPHLQEVLSPVTTPTDEERQHHHIRHVAGSTQTRHVPEEKPSHLQSISKLLLVISCVLVLLVMLNAMLFYKLWVLEYSTQALTTWQGLRLQDSHLPQSQAEWTQLLESQQKHHDTEMQKWKQIIKSSVMLLDQMKDSLINLQNGIISRDFVSESEEKQYQ

Target ORF information:

RefSeq Version	XM_031905706.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905706.1

ORF Insert Sequence:

ATGGTGGAGA AGAGCTCAGA TCATTCTTCA GACAAATCTC CTCCTACACC AGAACCTGGT 
GTGCAACGAA GTGGATCGTC TCAGTCTGGA CGTAGCAGTG GCAAAAATTC AAAGTATGAC 
AGACTTAACC TCATCAAAAA AAGCCAGAGC TGGTACAATG TACTTAGTCC AACATACAAA 
CAAAGAAATG AAGATTTTCG AAAACTTTTT AAACAGCTAC CAGATTCTGA GCGTCTCATT 
GTAGATTATT CTTGTGCTTT GCAAAGGGAC ATCCTTTTGC AAGGGCGGCT CTACCTGTCT 
GAGAATTGGA TCTGTTTCTA CAGCAACATC TTCCGTTGGG AGACATTGCT AACAGTACGA 
TTAAAAGATA TCTGTTCGAT GACCAAGGAG AAGACTGCCA GACTTATTCC AAATGCCATC 
CAACTCTGCA CTGAAACTGA AAAGCACTTT TTTACATCAT TTGGGGCTCG TGATCGAACC 
TACATGATGA TGTTCCGGCT TTGGCAGAAT GCTTTGCTGG AAAAGCCTCT GTGTCCAAAG 
GAGCTCTGGC ATTTTGTCCA TCAGTGTTAC GGGAATGAGC TAGGGCTTAC AAGTGATGAC 
GAAGACTATG TGCCTCCAGA TGATGACTTT AATACAATGG GATTCTGTGA AGAAATTCCT 
GTGGAAGAAG TGGAAGTAGT AAACTACAAT TCATCAAAAA GCAGCAATGA GGTAAAACCA 
GAGACCAACT CAAACCTACA GGACCCATCT TTAACTAGTA GCAATGTGAC ATCCACTGCC 
AGCAGTGATG CCCCAGCATT TGATGGGGGA ATGCTAGATG AGATCGACAG CTCATTAGAT 
AAGGAGATAT TAATTGCAAA TCTTGAGGAA CAGAAGGTGG TACTTCTTCC TCCTGTGACT 
TCACCATCCT TGGACTTTAA TGATAATGAA GATCTCCCCA CTGAGTTGAG CGATTCCTCA 
GATACCCATG ATGAAGGTGA AGTGCAGGCC TTTTATGAGG ATCTGAATGG TCGACAGTAT 
ATCAATGAGG TATTTAGTTT TAATGTGGAC AAGCTCTTCA GCCTTCTTTT CACTGAATCT 
CAGTTCCAAA GAGACTTCAT GGAACAGAGA CGCTTCACGG AAGTAATATT TCACCCCTGG 
AAGAAGGAAG AAAATGGCAA CCAGACAAGA GTTATTCTTT ACACCATTGC ACTTTCTAAC 
CCATTAGCAC CCAAATCAGC CACAGTCACA GAGAATCAGA CATTGTACAA GGCAAGCCAA 
GAGAGTGAGT GCTATGTGGT AGATGCCGAA GTTCAGACTC ACGATATTCC TTATCATGAT 
TACTTTTACA CCATCAATCG GTACACATTG ACAAGAGTGG CCAGGAATAA GAGCAGACTC 
AGGATCTCAA CAGAGTTACG TTATCGGAAA CAACCATGGG GTCTTGTCAA AACCTTCATA 
GAAAAAAACT TTTGGAGTGG ACTTGATGAG TATTTTCATC ATTTAGAGAA TGAACTGACC 
AAAGCAGAGA CGGCATACTT ATCTGATCTA CATCAACAGT CCCCCAAAGT AAAAAGCAGC 
AAAATCACTT CCACGCGCAG AAGGAAACGT CCCCATACTC ACCTGCGCAT CCCACATCTA 
CAGGAAGTCT TGAGCCCAGT TACAACTCCC ACCGATGAAG AGAGGCAGCA TCATCACATC 
AGACATGTTG CAGGTTCTAC TCAAACGCGG CATGTTCCAG AAGAGAAACC AAGTCATTTA 
CAAAGCATTT CTAAACTTCT GCTGGTAATC AGCTGTGTTT TGGTGCTGCT AGTGATGCTC 
AATGCAATGC TATTTTATAA GCTGTGGGTG CTGGAATATA GTACACAAGC ACTGACAACA 
TGGCAAGGCC TCAGGCTTCA GGACAGCCAC CTGCCTCAAT CACAAGCTGA GTGGACACAG 
TTGTTAGAAT CACAACAGAA GCACCATGAC ACTGAAATGC AAAAATGGAA GCAAATCATT 
AAGTCTTCTG TGATGCTGCT GGACCAGATG AAGGATTCGC TAATAAACCT TCAGAATGGA 
ATAATTTCCC GAGACTTTGT TTCAGAATCA GAAGAAAAAC AGTACCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gramd1b ( XM_031905707.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761567.1 Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X3, mRNA.

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CloneID	OXa62122
Clone ID Related Accession (Same CDS sequence)	XM_031905707.1
Accession Version	XM_031905707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Aster-B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAGA AGAGCTCAGA TCATTCTTCA GACAAATCTC CTCCTACACC AGAACCTGGT 
GTGCAACGAA GTGGATCGTC TCAGTCTGGA CGTAGCAGTG GCAAAAATTC AAAGAAAAGC 
CAGAGCTGGT ACAATGTACT TAGTCCAACA TACAAACAAA GAAATGAAGA TTTTCGAAAA 
CTTTTTAAAC AGCTACCAGA TTCTGAGCGT CTCATTGTAG ATTATTCTTG TGCTTTGCAA 
AGGGACATCC TTTTGCAAGG GCGGCTCTAC CTGTCTGAGA ATTGGATCTG TTTCTACAGC 
AACATCTTCC GTTGGGAGAC ATTGCTAACA GTACGATTAA AAGATATCTG TTCGATGACC 
AAGGAGAAGA CTGCCAGACT TATTCCAAAT GCCATCCAAC TCTGCACTGA AACTGAAAAG 
CACTTTTTTA CATCATTTGG GGCTCGTGAT CGAACCTACA TGATGATGTT CCGGCTTTGG 
CAGAATGCTT TGCTGGAAAA GCCTCTGTGT CCAAAGGAGC TCTGGCATTT TGTCCATCAG 
TGTTACGGGA ATGAGCTAGG GCTTACAAGT GATGACGAAG ACTATGTGCC TCCAGATGAT 
GACTTTAATA CAATGGGATT CTGTGAAGAA ATTCCTGTGG AAGAAGTGGA AGTAGTAAAC 
TACAATTCAT CAAAAAGCAG CAATGAGGTA AAACCAGAGA CCAACTCAAA CCTACAGGAC 
CCATCTTTAA CTAGTAGCAA TGTGACATCC ACTGCCAGCA GTGATGCCCC AGCATTTGAT 
GGGGGAATGC TAGATGAGAT CGACAGCTCA TTAGATAAGG AGATATTAAT TGCAAATCTT 
GAGGAACAGA AGGTGGTACT TCTTCCTCCT GTGACTTCAC CATCCTTGGA CTTTAATGAT 
AATGAAGATC TCCCCACTGA GTTGAGCGAT TCCTCAGATA CCCATGATGA AGGTGAAGTG 
CAGGCCTTTT ATGAGGATCT GAATGGTCGA CAGTATATCA ATGAGGTATT TAGTTTTAAT 
GTGGACAAGC TCTTCAGCCT TCTTTTCACT GAATCTCAGT TCCAAAGAGA CTTCATGGAA 
CAGAGACGCT TCACGGAAGT AATATTTCAC CCCTGGAAGA AGGAAGAAAA TGGCAACCAG 
ACAAGAGTTA TTCTTTACAC CATTGCACTT TCTAACCCAT TAGCACCCAA ATCAGCCACA 
GTCACAGAGA ATCAGACATT GTACAAGGCA AGCCAAGAGA GTGAGTGCTA TGTGGTAGAT 
GCCGAAGTTC AGACTCACGA TATTCCTTAT CATGATTACT TTTACACCAT CAATCGGTAC 
ACATTGACAA GAGTGGCCAG GAATAAGAGC AGACTCAGGA TCTCAACAGA GTTACGTTAT 
CGGAAACAAC CATGGGGTCT TGTCAAAACC TTCATAGAAA AAAACTTTTG GAGTGGACTT 
GATGAGTATT TTCATCATTT AGAGAATGAA CTGACCAAAG CAGAGACGGC ATACTTATCT 
GATCTACATC AACAGTCCCC CAAAGTAAAA AGCAGCAAAA TCACTTCCAC GCGCAGAAGG 
AAACGTCCCC ATACTCACCT GCGCATCCCA CATCTACAGG AAGTCTTGAG CCCAGTTACA 
ACTCCCACCG ATGAAGAGAG GCAGCATCAT CACATCAGAC ATGTTGCAGG TTCTACTCAA 
ACGCGGCATG TTCCAGAAGA GAAACCAAGT CATTTACAAA GCATTTCTAA ACTTCTGCTG 
GTAATCAGCT GTGTTTTGGT GCTGCTAGTG ATGCTCAATG CAATGCTATT TTATAAGCTG 
TGGGTGCTGG AATATAGTAC ACAAGCACTG ACAACATGGC AAGGCCTCAG GCTTCAGGAC 
AGCCACCTGC CTCAATCACA AGCTGAGTGG ACACAGTTGT TAGAATCACA ACAGAAGCAC 
CATGACACTG AAATGCAAAA ATGGAAGCAA ATCATTAAGT CTTCTGTGAT GCTGCTGGAC 
CAGATGAAGG ATTCGCTAAT AAACCTTCAG AATGGAATAA TTTCCCGAGA CTTTGTTTCA 
GAATCAGAAG AAAAACAGTA CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761567.1
CDS	175..2241
Translation	MVEKSSDHSSDKSPPTPEPGVQRSGSSQSGRSSGKNSKKSQSWYNVLSPTYKQRNEDFRKLFKQLPDSERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGFCEEIPVEEVEVVNYNSSKSSNEVKPETNSNLQDPSLTSSNVTSTASSDAPAFDGGMLDEIDSSLDKEILIANLEEQKVVLLPPVTSPSLDFNDNEDLPTELSDSSDTHDEGEVQAFYEDLNGRQYINEVFSFNVDKLFSLLFTESQFQRDFMEQRRFTEVIFHPWKKEENGNQTRVILYTIALSNPLAPKSATVTENQTLYKASQESECYVVDAEVQTHDIPYHDYFYTINRYTLTRVARNKSRLRISTELRYRKQPWGLVKTFIEKNFWSGLDEYFHHLENELTKAETAYLSDLHQQSPKVKSSKITSTRRRKRPHTHLRIPHLQEVLSPVTTPTDEERQHHHIRHVAGSTQTRHVPEEKPSHLQSISKLLLVISCVLVLLVMLNAMLFYKLWVLEYSTQALTTWQGLRLQDSHLPQSQAEWTQLLESQQKHHDTEMQKWKQIIKSSVMLLDQMKDSLINLQNGIISRDFVSESEEKQYQ

Target ORF information:

RefSeq Version	XM_031905707.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1B (gramd1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905707.1

ORF Insert Sequence:

ATGGTGGAGA AGAGCTCAGA TCATTCTTCA GACAAATCTC CTCCTACACC AGAACCTGGT 
GTGCAACGAA GTGGATCGTC TCAGTCTGGA CGTAGCAGTG GCAAAAATTC AAAGAAAAGC 
CAGAGCTGGT ACAATGTACT TAGTCCAACA TACAAACAAA GAAATGAAGA TTTTCGAAAA 
CTTTTTAAAC AGCTACCAGA TTCTGAGCGT CTCATTGTAG ATTATTCTTG TGCTTTGCAA 
AGGGACATCC TTTTGCAAGG GCGGCTCTAC CTGTCTGAGA ATTGGATCTG TTTCTACAGC 
AACATCTTCC GTTGGGAGAC ATTGCTAACA GTACGATTAA AAGATATCTG TTCGATGACC 
AAGGAGAAGA CTGCCAGACT TATTCCAAAT GCCATCCAAC TCTGCACTGA AACTGAAAAG 
CACTTTTTTA CATCATTTGG GGCTCGTGAT CGAACCTACA TGATGATGTT CCGGCTTTGG 
CAGAATGCTT TGCTGGAAAA GCCTCTGTGT CCAAAGGAGC TCTGGCATTT TGTCCATCAG 
TGTTACGGGA ATGAGCTAGG GCTTACAAGT GATGACGAAG ACTATGTGCC TCCAGATGAT 
GACTTTAATA CAATGGGATT CTGTGAAGAA ATTCCTGTGG AAGAAGTGGA AGTAGTAAAC 
TACAATTCAT CAAAAAGCAG CAATGAGGTA AAACCAGAGA CCAACTCAAA CCTACAGGAC 
CCATCTTTAA CTAGTAGCAA TGTGACATCC ACTGCCAGCA GTGATGCCCC AGCATTTGAT 
GGGGGAATGC TAGATGAGAT CGACAGCTCA TTAGATAAGG AGATATTAAT TGCAAATCTT 
GAGGAACAGA AGGTGGTACT TCTTCCTCCT GTGACTTCAC CATCCTTGGA CTTTAATGAT 
AATGAAGATC TCCCCACTGA GTTGAGCGAT TCCTCAGATA CCCATGATGA AGGTGAAGTG 
CAGGCCTTTT ATGAGGATCT GAATGGTCGA CAGTATATCA ATGAGGTATT TAGTTTTAAT 
GTGGACAAGC TCTTCAGCCT TCTTTTCACT GAATCTCAGT TCCAAAGAGA CTTCATGGAA 
CAGAGACGCT TCACGGAAGT AATATTTCAC CCCTGGAAGA AGGAAGAAAA TGGCAACCAG 
ACAAGAGTTA TTCTTTACAC CATTGCACTT TCTAACCCAT TAGCACCCAA ATCAGCCACA 
GTCACAGAGA ATCAGACATT GTACAAGGCA AGCCAAGAGA GTGAGTGCTA TGTGGTAGAT 
GCCGAAGTTC AGACTCACGA TATTCCTTAT CATGATTACT TTTACACCAT CAATCGGTAC 
ACATTGACAA GAGTGGCCAG GAATAAGAGC AGACTCAGGA TCTCAACAGA GTTACGTTAT 
CGGAAACAAC CATGGGGTCT TGTCAAAACC TTCATAGAAA AAAACTTTTG GAGTGGACTT 
GATGAGTATT TTCATCATTT AGAGAATGAA CTGACCAAAG CAGAGACGGC ATACTTATCT 
GATCTACATC AACAGTCCCC CAAAGTAAAA AGCAGCAAAA TCACTTCCAC GCGCAGAAGG 
AAACGTCCCC ATACTCACCT GCGCATCCCA CATCTACAGG AAGTCTTGAG CCCAGTTACA 
ACTCCCACCG ATGAAGAGAG GCAGCATCAT CACATCAGAC ATGTTGCAGG TTCTACTCAA 
ACGCGGCATG TTCCAGAAGA GAAACCAAGT CATTTACAAA GCATTTCTAA ACTTCTGCTG 
GTAATCAGCT GTGTTTTGGT GCTGCTAGTG ATGCTCAATG CAATGCTATT TTATAAGCTG 
TGGGTGCTGG AATATAGTAC ACAAGCACTG ACAACATGGC AAGGCCTCAG GCTTCAGGAC 
AGCCACCTGC CTCAATCACA AGCTGAGTGG ACACAGTTGT TAGAATCACA ACAGAAGCAC 
CATGACACTG AAATGCAAAA ATGGAAGCAA ATCATTAAGT CTTCTGTGAT GCTGCTGGAC 
CAGATGAAGG ATTCGCTAAT AAACCTTCAG AATGGAATAA TTTCCCGAGA CTTTGTTTCA 
GAATCAGAAG AAAAACAGTA CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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gramd1b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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