GRAMD1B cDNA ORF clone, Bos taurus(cattle)

The following GRAMD1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRAMD1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa222796 XM_024975271.1
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OBa222797 XM_024975272.1
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OBa26323 XM_005215954.2
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa26325 XM_005215956.2
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa49672 XM_010812451.1
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa49673 XM_010812453.2
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa238971 NM_001191419.3
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222790 XM_010812450.3
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222791 XM_024975268.1
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222792 XM_024975269.1
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222793 XM_005215954.3
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222794 XM_005215956.3
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222795 XM_024975270.1
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa92652 XM_015474650.1
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa92652 XM_015474651.1
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa02023 NM_001191419.1
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa216302 NM_001191419.2
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa49670 XM_010812449.2
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa49671 XM_010812450.2
Latest version!
Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa222796
    Clone ID Related Accession (Same CDS sequence) XM_024975271.1
    Accession Version XM_024975271.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA ACAGAGCAGC CAGCAGAGCA GCCAGCAGAG CAGCCATGAC
    GATGACTCCA GCAGGTTCCT GAGTCCTCGC GTGCGGGAAG AAAGCACTGC CAGTAACTCC
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGAAAAG CCAGAGCTGG TATAATGTGT TAAGCCCCAC ATACAAGCAG
    AGAAATGAAG ACTTCAGAAA GCTCTTTAAG CAACTTCCAG ACACGGAGCG CCTCATTGTT
    GATTACTCCT GTGCACTCCA AAGAGACATT CTTCTTCAGG GCCGACTCTA CCTCTCCGAA
    AACTGGATCT GCTTCTACAG CAACATCTTC CGCTGGGAAA CTCTGCTAAC TGTGCGTTTG
    AAAGACATCT GCTCCATGAC TAAAGAAAAG ACAGCTCGCC TCATCCCCAA TGCCATCCAA
    GTTTGCACTG ACTCAGAAAA GCATGTGGGA TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831039.1
    CDS48..1043
    Translation

    Target ORF information:

    RefSeq Version XM_024975271.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975271.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA ACAGAGCAGC CAGCAGAGCA GCCAGCAGAG CAGCCATGAC
    GATGACTCCA GCAGGTTCCT GAGTCCTCGC GTGCGGGAAG AAAGCACTGC CAGTAACTCC
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGAAAAG CCAGAGCTGG TATAATGTGT TAAGCCCCAC ATACAAGCAG
    AGAAATGAAG ACTTCAGAAA GCTCTTTAAG CAACTTCCAG ACACGGAGCG CCTCATTGTT
    GATTACTCCT GTGCACTCCA AAGAGACATT CTTCTTCAGG GCCGACTCTA CCTCTCCGAA
    AACTGGATCT GCTTCTACAG CAACATCTTC CGCTGGGAAA CTCTGCTAAC TGTGCGTTTG
    AAAGACATCT GCTCCATGAC TAAAGAAAAG ACAGCTCGCC TCATCCCCAA TGCCATCCAA
    GTTTGCACTG ACTCAGAAAA GCATGTGGGA TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222797
    Clone ID Related Accession (Same CDS sequence) XM_024975272.1
    Accession Version XM_024975272.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCCCAGG TGGGGCCCGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831040.1
    CDS1410..1802
    Translation

    Target ORF information:

    RefSeq Version XM_024975272.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975272.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCCCAGG TGGGGCCCGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26323
    Clone ID Related Accession (Same CDS sequence) XM_005215954.2
    Accession Version XM_005215954.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005215954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTGGGAAC ACACTGAATC AGAGTATCTG CATGAGGCCC ACGGGCTGAA GCAAGGCTGC 
    TTCTCTTTAA TGGTTAAGGG TGGATATCCT GGAGGTGACC CACTGGCCTT GACTCTGGGG
    CCCACCAGCT ATTATCTCTG TGATCTTGGC ACTGCCAGTA ACTCCAACCG CAGCACGCCA
    GCCTGCTCCC CCATCCTCCG GAAGCGGTCT CGCTCACCAA CCCCGCAGAA CGTAGACGGA
    GACACCATGG TGGAGAAGGG CTCAGATCAC TCCTCGGACA AGTCGCCGTC CACTCCGGAG
    CAGGGCGTGC AGCGCAGCTG CTCCTCACAG TCCGGCCGCA GTGGTGGCAA AAACTCCAAG
    AAAAGCCAGA GCTGGTATAA TGTGTTAAGC CCCACATACA AGCAGAGAAA TGAAGACTTC
    AGAAAGCTCT TTAAGCAACT TCCAGACACG GAGCGCCTCA TTGTTGATTA CTCCTGTGCA
    CTCCAAAGAG ACATTCTTCT TCAGGGCCGA CTCTACCTCT CCGAAAACTG GATCTGCTTC
    TACAGCAACA TCTTCCGCTG GGAAACTCTG CTAACTGTGC GTTTGAAAGA CATCTGCTCC
    ATGACTAAAG AAAAGACAGC TCGCCTCATC CCCAATGCCA TCCAAGTTTG CACTGACTCA
    GAAAAGCACT TTTTCACTTC GTTCGGGGCC CGGGATCGGA CGTACATGAT GATGTTCCGG
    CTCTGGCAGA ATGCTCTCCT GGAAAAGCCC CTGTGCCCCA AGGAGCTGTG GCACTTCGTC
    CACCAGTGCT ATGGGAACGA GCTGGGCCTG ACCAGTGACG ACGAGGACTA CGTGCCCCCC
    GATGACGACT TCAACACGAT GGGCTACTGT GAGGAGATCC CCGTAGAAGA GAATGAAGTG
    AACGACAGCT CATCCAAAAG CAGCATAGAG ACCAAGCCAG ATGCCAGTCC ACAGCTGCCC
    AAGAAATCCA TCACCAACAG CACACTGACG TCCACCGGGA GCAGTGAAGC CCCCGTCTCG
    TTTGACGGCC TGCCCCTGGA GGAGGAGGTG CTAGAGGGCG ACGGGTCTCT GGAGAAGGAG
    CTCGCCATCG ACAACATCAT CGGGGAAAAG ATTGAGATCA TCGCGCCCGT GAACTCCCCT
    TCGCTGGACT TCAACGACAA CGAGGACATC CCCACTGAGC TCAGTGACTC TTCAGACACC
    CACGACGAAG GGGAGGTCCA AGCCTTCTAC GAGGACTTGA GTGGCCGGCA GTACGTGAAC
    GAAGTCTTCA ACTTCAGCGT GGACAAGCTC TATGACCTCC TGTTCACTGA CTCACCCTTC
    CAGCGGGACT TCATGGAGCA GCGGCGCTTC TCAGATATCA TCTTCCATCC GTGGAAGAAG
    GAAGAGAATG GAAACCAGAG CCGAGTGATC CTTTACACCA TCACCCTTAC CAACCCTCTA
    GCTCCCAAAA CTGCCACCGT CAGGGAGACA CAGACCATGT ACAAGGCGAG CCAGGAGAGC
    GAGTGCTATG TGATCGACGC CGAGGTCCTC ACGCACGATG TACCGTATCA CGACTACTTC
    TACACCATCA ACCGCTACAC GCTTACCCGC GTGGCCCGCA ACAAGAGTCG ACTCAGGGTC
    TCCACGGAGC TGCGCTATCG GAAACAGCCC TGGGGGCTGG TGAAAACATT CATCGAGAAG
    AACTTCTGGA GTGGGCTGGA GGACTACTTC CGCCACCTGG AGAGTGAGCT GACCAAAACA
    GAGAGCACCT ACCTGGCGGA GATGCACAGA CAGTCTCCTA AAGAGAAGGC CAGCAAGCCC
    CCAACGGTGC GGAGGAGAAA ACGGCCCCAT GCCCACCTGC GGGTGCCACA CCTGGAGGAG
    GTGATGAGCC CCGTCACCAC GCCCACTGAT GAGGACGTGG GCCACAGGAT CAAACATGTG
    GCAGGTTCCA CACAGACACG GCACATCCCC GAGGACGGCC CCAGCGGTTT CCACCTGCAG
    AGCGTGTCCA AGCTGCTGCT GGTTATCAGC TGTGTTCTGG TGCTGCTGGT CATCCTCAAC
    GTGATGCTCT TCTACAAACT CTGGATGCTG GAGTACACCA CCCAGACCCT CACTGCCTGG
    CAGGGTCTCC GGCTCCAAGA AAGGTTACCC CAGTCCCAGA CAGAGTGGGC CCAGCTCTTA
    GAGTCCCAGC AAAAGTACCA CGATACTGAG CTCCAGAAAT GGAGGGAGAT CATCAAATCC
    TCGGTGATGC TTCTTGACCA GATGAAGGAC TCACTCATCA ATCTTCAGAA CGGCATCAGG
    TCCCGAGACT ACACGTCAGA AAGCGAAGAG AAGAGGAACC GGTACCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216011.1
    CDS45..2375
    Translation

    Target ORF information:

    RefSeq Version XM_005215954.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215954.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTGGGAAC ACACTGAATC AGAGTATCTG CATGAGGCCC ACGGGCTGAA GCAAGGCTGC 
    TTCTCTTTAA TGGTTAAGGG TGGATATCCT GGAGGTGACC CACTGGCCTT GACTCTGGGG
    CCCACCAGCT ATTATCTCTG TGATCTTGGC ACTGCCAGTA ACTCCAACCG CAGCACGCCA
    GCCTGCTCCC CCATCCTCCG GAAGCGGTCT CGCTCACCAA CCCCGCAGAA CGTAGACGGA
    GACACCATGG TGGAGAAGGG CTCAGATCAC TCCTCGGACA AGTCGCCGTC CACTCCGGAG
    CAGGGCGTGC AGCGCAGCTG CTCCTCACAG TCCGGCCGCA GTGGTGGCAA AAACTCCAAG
    AAAAGCCAGA GCTGGTATAA TGTGTTAAGC CCCACATACA AGCAGAGAAA TGAAGACTTC
    AGAAAGCTCT TTAAGCAACT TCCAGACACG GAGCGCCTCA TTGTTGATTA CTCCTGTGCA
    CTCCAAAGAG ACATTCTTCT TCAGGGCCGA CTCTACCTCT CCGAAAACTG GATCTGCTTC
    TACAGCAACA TCTTCCGCTG GGAAACTCTG CTAACTGTGC GTTTGAAAGA CATCTGCTCC
    ATGACTAAAG AAAAGACAGC TCGCCTCATC CCCAATGCCA TCCAAGTTTG CACTGACTCA
    GAAAAGCACT TTTTCACTTC GTTCGGGGCC CGGGATCGGA CGTACATGAT GATGTTCCGG
    CTCTGGCAGA ATGCTCTCCT GGAAAAGCCC CTGTGCCCCA AGGAGCTGTG GCACTTCGTC
    CACCAGTGCT ATGGGAACGA GCTGGGCCTG ACCAGTGACG ACGAGGACTA CGTGCCCCCC
    GATGACGACT TCAACACGAT GGGCTACTGT GAGGAGATCC CCGTAGAAGA GAATGAAGTG
    AACGACAGCT CATCCAAAAG CAGCATAGAG ACCAAGCCAG ATGCCAGTCC ACAGCTGCCC
    AAGAAATCCA TCACCAACAG CACACTGACG TCCACCGGGA GCAGTGAAGC CCCCGTCTCG
    TTTGACGGCC TGCCCCTGGA GGAGGAGGTG CTAGAGGGCG ACGGGTCTCT GGAGAAGGAG
    CTCGCCATCG ACAACATCAT CGGGGAAAAG ATTGAGATCA TCGCGCCCGT GAACTCCCCT
    TCGCTGGACT TCAACGACAA CGAGGACATC CCCACTGAGC TCAGTGACTC TTCAGACACC
    CACGACGAAG GGGAGGTCCA AGCCTTCTAC GAGGACTTGA GTGGCCGGCA GTACGTGAAC
    GAAGTCTTCA ACTTCAGCGT GGACAAGCTC TATGACCTCC TGTTCACTGA CTCACCCTTC
    CAGCGGGACT TCATGGAGCA GCGGCGCTTC TCAGATATCA TCTTCCATCC GTGGAAGAAG
    GAAGAGAATG GAAACCAGAG CCGAGTGATC CTTTACACCA TCACCCTTAC CAACCCTCTA
    GCTCCCAAAA CTGCCACCGT CAGGGAGACA CAGACCATGT ACAAGGCGAG CCAGGAGAGC
    GAGTGCTATG TGATCGACGC CGAGGTCCTC ACGCACGATG TACCGTATCA CGACTACTTC
    TACACCATCA ACCGCTACAC GCTTACCCGC GTGGCCCGCA ACAAGAGTCG ACTCAGGGTC
    TCCACGGAGC TGCGCTATCG GAAACAGCCC TGGGGGCTGG TGAAAACATT CATCGAGAAG
    AACTTCTGGA GTGGGCTGGA GGACTACTTC CGCCACCTGG AGAGTGAGCT GACCAAAACA
    GAGAGCACCT ACCTGGCGGA GATGCACAGA CAGTCTCCTA AAGAGAAGGC CAGCAAGCCC
    CCAACGGTGC GGAGGAGAAA ACGGCCCCAT GCCCACCTGC GGGTGCCACA CCTGGAGGAG
    GTGATGAGCC CCGTCACCAC GCCCACTGAT GAGGACGTGG GCCACAGGAT CAAACATGTG
    GCAGGTTCCA CACAGACACG GCACATCCCC GAGGACGGCC CCAGCGGTTT CCACCTGCAG
    AGCGTGTCCA AGCTGCTGCT GGTTATCAGC TGTGTTCTGG TGCTGCTGGT CATCCTCAAC
    GTGATGCTCT TCTACAAACT CTGGATGCTG GAGTACACCA CCCAGACCCT CACTGCCTGG
    CAGGGTCTCC GGCTCCAAGA AAGGTTACCC CAGTCCCAGA CAGAGTGGGC CCAGCTCTTA
    GAGTCCCAGC AAAAGTACCA CGATACTGAG CTCCAGAAAT GGAGGGAGAT CATCAAATCC
    TCGGTGATGC TTCTTGACCA GATGAAGGAC TCACTCATCA ATCTTCAGAA CGGCATCAGG
    TCCCGAGACT ACACGTCAGA AAGCGAAGAG AAGAGGAACC GGTACCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26325
    Clone ID Related Accession (Same CDS sequence) XM_005215956.2
    Accession Version XM_005215956.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005215956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCTCTG CACTGTGCTC CACTACCTTC CCAATGGCTG CCAGCACTGC CAGTAACTCC 
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGAAAAG CCAGAGCTGG TATAATGTGT TAAGCCCCAC ATACAAGCAG
    AGAAATGAAG ACTTCAGAAA GCTCTTTAAG CAACTTCCAG ACACGGAGCG CCTCATTGTT
    GATTACTCCT GTGCACTCCA AAGAGACATT CTTCTTCAGG GCCGACTCTA CCTCTCCGAA
    AACTGGATCT GCTTCTACAG CAACATCTTC CGCTGGGAAA CTCTGCTAAC TGTGCGTTTG
    AAAGACATCT GCTCCATGAC TAAAGAAAAG ACAGCTCGCC TCATCCCCAA TGCCATCCAA
    GTTTGCACTG ACTCAGAAAA GCACTTTTTC ACTTCGTTCG GGGCCCGGGA TCGGACGTAC
    ATGATGATGT TCCGGCTCTG GCAGAATGCT CTCCTGGAAA AGCCCCTGTG CCCCAAGGAG
    CTGTGGCACT TCGTCCACCA GTGCTATGGG AACGAGCTGG GCCTGACCAG TGACGACGAG
    GACTACGTGC CCCCCGATGA CGACTTCAAC ACGATGGGCT ACTGTGAGGA GATCCCCGTA
    GAAGAGAATG AAGTGAACGA CAGCTCATCC AAAAGCAGCA TAGAGACCAA GCCAGATGCC
    AGTCCACAGC TGCCCAAGAA ATCCATCACC AACAGCACAC TGACGTCCAC CGGGAGCAGT
    GAAGCCCCCG TCTCGTTTGA CGGCCTGCCC CTGGAGGAGG AGGTGCTAGA GGGCGACGGG
    TCTCTGGAGA AGGAGCTCGC CATCGACAAC ATCATCGGGG AAAAGATTGA GATCATCGCG
    CCCGTGAACT CCCCTTCGCT GGACTTCAAC GACAACGAGG ACATCCCCAC TGAGCTCAGT
    GACTCTTCAG ACACCCACGA CGAAGGGGAG GTCCAAGCCT TCTACGAGGA CTTGAGTGGC
    CGGCAGTACG TGAACGAAGT CTTCAACTTC AGCGTGGACA AGCTCTATGA CCTCCTGTTC
    ACTGACTCAC CCTTCCAGCG GGACTTCATG GAGCAGCGGC GCTTCTCAGA TATCATCTTC
    CATCCGTGGA AGAAGGAAGA GAATGGAAAC CAGAGCCGAG TGATCCTTTA CACCATCACC
    CTTACCAACC CTCTAGCTCC CAAAACTGCC ACCGTCAGGG AGACACAGAC CATGTACAAG
    GCGAGCCAGG AGAGCGAGTG CTATGTGATC GACGCCGAGG TCCTCACGCA CGATGTACCG
    TATCACGACT ACTTCTACAC CATCAACCGC TACACGCTTA CCCGCGTGGC CCGCAACAAG
    AGTCGACTCA GGGTCTCCAC GGAGCTGCGC TATCGGAAAC AGCCCTGGGG GCTGGTGAAA
    ACATTCATCG AGAAGAACTT CTGGAGTGGG CTGGAGGACT ACTTCCGCCA CCTGGAGAGT
    GAGCTGACCA AAACAGAGAG CACCTACCTG GCGGAGATGC ACAGACAGTC TCCTAAAGAG
    AAGGCCAGCA AGCCCCCAAC GGTGCGGAGG AGAAAACGGC CCCATGCCCA CCTGCGGGTG
    CCACACCTGG AGGAGGTGAT GAGCCCCGTC ACCACGCCCA CTGATGAGGA CGTGGGCCAC
    AGGATCAAAC ATGTGGCAGG TTCCACACAG ACACGGCACA TCCCCGAGGA CGGCCCCAGC
    GGTTTCCACC TGCAGAGCGT GTCCAAGCTG CTGCTGGTTA TCAGCTGTGT TCTGGTGCTG
    CTGGTCATCC TCAACGTGAT GCTCTTCTAC AAACTCTGGA TGCTGGAGTA CACCACCCAG
    ACCCTCACTG CCTGGCAGGG TCTCCGGCTC CAAGAAAGGT TACCCCAGTC CCAGACAGAG
    TGGGCCCAGC TCTTAGAGTC CCAGCAAAAG TACCACGATA CTGAGCTCCA GAAATGGAGG
    GAGATCATCA AATCCTCGGT GATGCTTCTT GACCAGATGA AGGACTCACT CATCAATCTT
    CAGAACGGCA TCAGGTCCCG AGACTACACG TCAGAAAGCG AAGAGAAGAG GAACCGGTAC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216013.1
    CDS244..2469
    Translation

    Target ORF information:

    RefSeq Version XM_005215956.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215956.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCTCTG CACTGTGCTC CACTACCTTC CCAATGGCTG CCAGCACTGC CAGTAACTCC 
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGAAAAG CCAGAGCTGG TATAATGTGT TAAGCCCCAC ATACAAGCAG
    AGAAATGAAG ACTTCAGAAA GCTCTTTAAG CAACTTCCAG ACACGGAGCG CCTCATTGTT
    GATTACTCCT GTGCACTCCA AAGAGACATT CTTCTTCAGG GCCGACTCTA CCTCTCCGAA
    AACTGGATCT GCTTCTACAG CAACATCTTC CGCTGGGAAA CTCTGCTAAC TGTGCGTTTG
    AAAGACATCT GCTCCATGAC TAAAGAAAAG ACAGCTCGCC TCATCCCCAA TGCCATCCAA
    GTTTGCACTG ACTCAGAAAA GCACTTTTTC ACTTCGTTCG GGGCCCGGGA TCGGACGTAC
    ATGATGATGT TCCGGCTCTG GCAGAATGCT CTCCTGGAAA AGCCCCTGTG CCCCAAGGAG
    CTGTGGCACT TCGTCCACCA GTGCTATGGG AACGAGCTGG GCCTGACCAG TGACGACGAG
    GACTACGTGC CCCCCGATGA CGACTTCAAC ACGATGGGCT ACTGTGAGGA GATCCCCGTA
    GAAGAGAATG AAGTGAACGA CAGCTCATCC AAAAGCAGCA TAGAGACCAA GCCAGATGCC
    AGTCCACAGC TGCCCAAGAA ATCCATCACC AACAGCACAC TGACGTCCAC CGGGAGCAGT
    GAAGCCCCCG TCTCGTTTGA CGGCCTGCCC CTGGAGGAGG AGGTGCTAGA GGGCGACGGG
    TCTCTGGAGA AGGAGCTCGC CATCGACAAC ATCATCGGGG AAAAGATTGA GATCATCGCG
    CCCGTGAACT CCCCTTCGCT GGACTTCAAC GACAACGAGG ACATCCCCAC TGAGCTCAGT
    GACTCTTCAG ACACCCACGA CGAAGGGGAG GTCCAAGCCT TCTACGAGGA CTTGAGTGGC
    CGGCAGTACG TGAACGAAGT CTTCAACTTC AGCGTGGACA AGCTCTATGA CCTCCTGTTC
    ACTGACTCAC CCTTCCAGCG GGACTTCATG GAGCAGCGGC GCTTCTCAGA TATCATCTTC
    CATCCGTGGA AGAAGGAAGA GAATGGAAAC CAGAGCCGAG TGATCCTTTA CACCATCACC
    CTTACCAACC CTCTAGCTCC CAAAACTGCC ACCGTCAGGG AGACACAGAC CATGTACAAG
    GCGAGCCAGG AGAGCGAGTG CTATGTGATC GACGCCGAGG TCCTCACGCA CGATGTACCG
    TATCACGACT ACTTCTACAC CATCAACCGC TACACGCTTA CCCGCGTGGC CCGCAACAAG
    AGTCGACTCA GGGTCTCCAC GGAGCTGCGC TATCGGAAAC AGCCCTGGGG GCTGGTGAAA
    ACATTCATCG AGAAGAACTT CTGGAGTGGG CTGGAGGACT ACTTCCGCCA CCTGGAGAGT
    GAGCTGACCA AAACAGAGAG CACCTACCTG GCGGAGATGC ACAGACAGTC TCCTAAAGAG
    AAGGCCAGCA AGCCCCCAAC GGTGCGGAGG AGAAAACGGC CCCATGCCCA CCTGCGGGTG
    CCACACCTGG AGGAGGTGAT GAGCCCCGTC ACCACGCCCA CTGATGAGGA CGTGGGCCAC
    AGGATCAAAC ATGTGGCAGG TTCCACACAG ACACGGCACA TCCCCGAGGA CGGCCCCAGC
    GGTTTCCACC TGCAGAGCGT GTCCAAGCTG CTGCTGGTTA TCAGCTGTGT TCTGGTGCTG
    CTGGTCATCC TCAACGTGAT GCTCTTCTAC AAACTCTGGA TGCTGGAGTA CACCACCCAG
    ACCCTCACTG CCTGGCAGGG TCTCCGGCTC CAAGAAAGGT TACCCCAGTC CCAGACAGAG
    TGGGCCCAGC TCTTAGAGTC CCAGCAAAAG TACCACGATA CTGAGCTCCA GAAATGGAGG
    GAGATCATCA AATCCTCGGT GATGCTTCTT GACCAGATGA AGGACTCACT CATCAATCTT
    CAGAACGGCA TCAGGTCCCG AGACTACACG TCAGAAAGCG AAGAGAAGAG GAACCGGTAC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49672
    Clone ID Related Accession (Same CDS sequence) XM_010812451.1
    Accession Version XM_010812451.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCTCTG CACTGTGCTC CACTACCTTC CCAATGGCTG CCAGCACTGC CAGTAACTCC 
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGTCTCA CAAGCGCCTC TCAAAATACG ACAGACTTTA CCTGATCAAA
    AAAAGCCAGA GCTGGTATAA TGTGTTAAGC CCCACATACA AGCAGAGAAA TGAAGACTTC
    AGAAAGCTCT TTAAGCAACT TCCAGACACG GAGCGCCTCA TTGTTGATTA CTCCTGTGCA
    CTCCAAAGAG ACATTCTTCT TCAGGGCCGA CTCTACCTCT CCGAAAACTG GATCTGCTTC
    TACAGCAACA TCTTCCGCTG GGAAACTCTG CTAACTGTGC GTTTGAAAGA CATCTGCTCC
    ATGACTAAAG AAAAGACAGC TCGCCTCATC CCCAATGCCA TCCAAGTTTG CACTGACTCA
    GAAAAGCACT TTTTCACTTC GTTCGGGGCC CGGGATCGGA CGTACATGAT GATGTTCCGG
    CTCTGGCAGA ATGCTCTCCT GGAAAAGCCC CTGTGCCCCA AGGAGCTGTG GCACTTCGTC
    CACCAGTGCT ATGGGAACGA GCTGGGCCTG ACCAGTGACG ACGAGGACTA CGTGCCCCCC
    GATGACGACT TCAACACGAT GGGCTACTGT GAGGAGATCC CCGTAGAAGA GAATGAAGTG
    AACGACAGCT CATCCAAAAG CAGCATAGAG ACCAAGCCAG ATGCCAGTCC ACAGCTGCCC
    AAGAAATCCA TCACCAACAG CACACTGACG TCCACCGGGA GCAGTGAAGC CCCCGTCTCG
    TTTGACGGCC TGCCCCTGGA GGAGGAGGTG CTAGAGGGCG ACGGGTCTCT GGAGAAGGAG
    CTCGCCATCG ACAACATCAT CGGGGAAAAG ATTGAGATCA TCGCGCCCGT GAACTCCCCT
    TCGCTGGACT TCAACGACAA CGAGGACATC CCCACTGAGC TCAGTGACTC TTCAGACACC
    CACGACGAAG GGGAGGTCCA AGCCTTCTAC GAGGACTTGA GTGGCCGGCA GTACGTGAAC
    GAAGTCTTCA ACTTCAGCGT GGACAAGCTC TATGACCTCC TGTTCACTGA CTCACCCTTC
    CAGCGGGACT TCATGGAGCA GCGGCGCTTC TCAGATATCA TCTTCCATCC GTGGAAGAAG
    GAAGAGAATG GAAACCAGAG CCGAGTGATC CTTTACACCA TCACCCTTAC CAACCCTCTA
    GCTCCCAAAA CTGCCACCGT CAGGGAGACA CAGACCATGT ACAAGGCGAG CCAGGAGAGC
    GAGTGCTATG TGATCGACGC CGAGGTCCTC ACGCACGATG TACCGTATCA CGACTACTTC
    TACACCATCA ACCGCTACAC GCTTACCCGC GTGGCCCGCA ACAAGAGTCG ACTCAGGGTC
    TCCACGGAGC TGCGCTATCG GAAACAGCCC TGGGGGCTGG TGAAAACATT CATCGAGAAG
    AACTTCTGGA GTGGGCTGGA GGACTACTTC CGCCACCTGG AGAGTGAGCT GACCAAAACA
    GAGAGCACCT ACCTGGCGGA GATGCACAGA CAGTCTCCTA AAGAGAAGGC CAGCAAGCCC
    CCAACGGTGC GGAGGAGAAA ACGGCCCCAT GCCCACCTGC GGGTGCCACA CCTGGAGGAG
    GTGATGAGCC CCGTCACCAC GCCCACTGAT GAGGACGTGG GCCACAGGAT CAAACATGTG
    GCAGGTTCCA CACAGACACG GCACATCCCC GAGGACGGCC CCAGCGGTTT CCACCTGCAG
    AGCGTGTCCA AGCTGCTGCT GGTTATCAGC TGTGTTCTGG TGCTGCTGGT CATCCTCAAC
    GTGATGCTCT TCTACAAACT CTGGATGCTG GAGTACACCA CCCAGACCCT CACTGCCTGG
    CAGGGTCTCC GGCTCCAAGA AAGGTTACCC CAGTCCCAGA CAGAGTGGGC CCAGCTCTTA
    GAGTCCCAGC AAAAGTACCA CGATACTGAG CTCCAGAAAT GGAGGGAGAT CATCAAATCC
    TCGGTGATGC TTCTTGACCA GATGAAGGAC TCACTCATCA ATCTTCAGAA CGGCATCAGG
    TCCCGAGACT ACACGTCAGA AAGCGAAGAG AAGAGGAACC GGTACCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810753.1
    CDS244..2514
    Translation

    Target ORF information:

    RefSeq Version XM_010812451.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCTCTG CACTGTGCTC CACTACCTTC CCAATGGCTG CCAGCACTGC CAGTAACTCC 
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGTCTCA CAAGCGCCTC TCAAAATACG ACAGACTTTA CCTGATCAAA
    AAAAGCCAGA GCTGGTATAA TGTGTTAAGC CCCACATACA AGCAGAGAAA TGAAGACTTC
    AGAAAGCTCT TTAAGCAACT TCCAGACACG GAGCGCCTCA TTGTTGATTA CTCCTGTGCA
    CTCCAAAGAG ACATTCTTCT TCAGGGCCGA CTCTACCTCT CCGAAAACTG GATCTGCTTC
    TACAGCAACA TCTTCCGCTG GGAAACTCTG CTAACTGTGC GTTTGAAAGA CATCTGCTCC
    ATGACTAAAG AAAAGACAGC TCGCCTCATC CCCAATGCCA TCCAAGTTTG CACTGACTCA
    GAAAAGCACT TTTTCACTTC GTTCGGGGCC CGGGATCGGA CGTACATGAT GATGTTCCGG
    CTCTGGCAGA ATGCTCTCCT GGAAAAGCCC CTGTGCCCCA AGGAGCTGTG GCACTTCGTC
    CACCAGTGCT ATGGGAACGA GCTGGGCCTG ACCAGTGACG ACGAGGACTA CGTGCCCCCC
    GATGACGACT TCAACACGAT GGGCTACTGT GAGGAGATCC CCGTAGAAGA GAATGAAGTG
    AACGACAGCT CATCCAAAAG CAGCATAGAG ACCAAGCCAG ATGCCAGTCC ACAGCTGCCC
    AAGAAATCCA TCACCAACAG CACACTGACG TCCACCGGGA GCAGTGAAGC CCCCGTCTCG
    TTTGACGGCC TGCCCCTGGA GGAGGAGGTG CTAGAGGGCG ACGGGTCTCT GGAGAAGGAG
    CTCGCCATCG ACAACATCAT CGGGGAAAAG ATTGAGATCA TCGCGCCCGT GAACTCCCCT
    TCGCTGGACT TCAACGACAA CGAGGACATC CCCACTGAGC TCAGTGACTC TTCAGACACC
    CACGACGAAG GGGAGGTCCA AGCCTTCTAC GAGGACTTGA GTGGCCGGCA GTACGTGAAC
    GAAGTCTTCA ACTTCAGCGT GGACAAGCTC TATGACCTCC TGTTCACTGA CTCACCCTTC
    CAGCGGGACT TCATGGAGCA GCGGCGCTTC TCAGATATCA TCTTCCATCC GTGGAAGAAG
    GAAGAGAATG GAAACCAGAG CCGAGTGATC CTTTACACCA TCACCCTTAC CAACCCTCTA
    GCTCCCAAAA CTGCCACCGT CAGGGAGACA CAGACCATGT ACAAGGCGAG CCAGGAGAGC
    GAGTGCTATG TGATCGACGC CGAGGTCCTC ACGCACGATG TACCGTATCA CGACTACTTC
    TACACCATCA ACCGCTACAC GCTTACCCGC GTGGCCCGCA ACAAGAGTCG ACTCAGGGTC
    TCCACGGAGC TGCGCTATCG GAAACAGCCC TGGGGGCTGG TGAAAACATT CATCGAGAAG
    AACTTCTGGA GTGGGCTGGA GGACTACTTC CGCCACCTGG AGAGTGAGCT GACCAAAACA
    GAGAGCACCT ACCTGGCGGA GATGCACAGA CAGTCTCCTA AAGAGAAGGC CAGCAAGCCC
    CCAACGGTGC GGAGGAGAAA ACGGCCCCAT GCCCACCTGC GGGTGCCACA CCTGGAGGAG
    GTGATGAGCC CCGTCACCAC GCCCACTGAT GAGGACGTGG GCCACAGGAT CAAACATGTG
    GCAGGTTCCA CACAGACACG GCACATCCCC GAGGACGGCC CCAGCGGTTT CCACCTGCAG
    AGCGTGTCCA AGCTGCTGCT GGTTATCAGC TGTGTTCTGG TGCTGCTGGT CATCCTCAAC
    GTGATGCTCT TCTACAAACT CTGGATGCTG GAGTACACCA CCCAGACCCT CACTGCCTGG
    CAGGGTCTCC GGCTCCAAGA AAGGTTACCC CAGTCCCAGA CAGAGTGGGC CCAGCTCTTA
    GAGTCCCAGC AAAAGTACCA CGATACTGAG CTCCAGAAAT GGAGGGAGAT CATCAAATCC
    TCGGTGATGC TTCTTGACCA GATGAAGGAC TCACTCATCA ATCTTCAGAA CGGCATCAGG
    TCCCGAGACT ACACGTCAGA AAGCGAAGAG AAGAGGAACC GGTACCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49673
    Clone ID Related Accession (Same CDS sequence) XM_010812453.2
    Accession Version XM_010812453.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2250bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAAAGGAT TCAAGCTGTC CTGCACTGCC AGTAACTCCA ACCGCAGCAC GCCAGCCTGC 
    TCCCCCATCC TCCGGAAGCG GTCTCGCTCA CCAACCCCGC AGAACGTAGA CGGAGACACC
    ATGGTGGAGA AGGGCTCAGA TCACTCCTCG GACAAGTCGC CGTCCACTCC GGAGCAGGGC
    GTGCAGCGCA GCTGCTCCTC ACAGTCCGGC CGCAGTGGTG GCAAAAACTC CAAGTCTCAC
    AAGCGCCTCT CAAAATACGA CAGACTTTAC CTGATCAAAA AAAGCCAGAG CTGGTATAAT
    GTGTTAAGCC CCACATACAA GCAGAGAAAT GAAGACTTCA GAAAGCTCTT TAAGCAACTT
    CCAGACACGG AGCGCCTCAT TGTTGATTAC TCCTGTGCAC TCCAAAGAGA CATTCTTCTT
    CAGGGCCGAC TCTACCTCTC CGAAAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG
    GAAACTCTGC TAACTGTGCG TTTGAAAGAC ATCTGCTCCA TGACTAAAGA AAAGACAGCT
    CGCCTCATCC CCAATGCCAT CCAAGTTTGC ACTGACTCAG AAAAGCACTT TTTCACTTCG
    TTCGGGGCCC GGGATCGGAC GTACATGATG ATGTTCCGGC TCTGGCAGAA TGCTCTCCTG
    GAAAAGCCCC TGTGCCCCAA GGAGCTGTGG CACTTCGTCC ACCAGTGCTA TGGGAACGAG
    CTGGGCCTGA CCAGTGACGA CGAGGACTAC GTGCCCCCCG ATGACGACTT CAACACGATG
    GGCTACTGTG AGGAGATCCC CGTAGAAGAG AATGAAGTGA ACGACAGCTC ATCCAAAAGC
    AGCATAGAGA CCAAGCCAGA TGCCAGTCCA CAGCTGCCCA AGAAATCCAT CACCAACAGC
    ACACTGACGT CCACCGGGAG CAGTGAAGCC CCCGTCTCGT TTGACGGCCT GCCCCTGGAG
    GAGGAGGTGC TAGAGGGCGA CGGGTCTCTG GAGAAGGAGC TCGCCATCGA CAACATCATC
    GGGGAAAAGA TTGAGATCAT CGCGCCCGTG AACTCCCCTT CGCTGGACTT CAACGACAAC
    GAGGACATCC CCACTGAGCT CAGTGACTCT TCAGACACCC ACGACGAAGG GGAGGTCCAA
    GCCTTCTACG AGGACTTGAG TGGCCGGCAG TACGTGAACG AAGTCTTCAA CTTCAGCGTG
    GACAAGCTCT ATGACCTCCT GTTCACTGAC TCACCCTTCC AGCGGGACTT CATGGAGCAG
    CGGCGCTTCT CAGATATCAT CTTCCATCCG TGGAAGAAGG AAGAGAATGG AAACCAGAGC
    CGAGTGATCC TTTACACCAT CACCCTTACC AACCCTCTAG CTCCCAAAAC TGCCACCGTC
    AGGGAGACAC AGACCATGTA CAAGGCGAGC CAGGAGAGCG AGTGCTATGT GATCGACGCC
    GAGGTCCTCA CGCACGATGT ACCGTATCAC GACTACTTCT ACACCATCAA CCGCTACACG
    CTTACCCGCG TGGCCCGCAA CAAGAGTCGA CTCAGGGTCT CCACGGAGCT GCGCTATCGG
    AAACAGCCCT GGGGGCTGGT GAAAACATTC ATCGAGAAGA ACTTCTGGAG TGGGCTGGAG
    GACTACTTCC GCCACCTGGA GAGTGAGCTG ACCAAAACAG AGAGCACCTA CCTGGCGGAG
    ATGCACAGAC AGTCTCCTAA AGAGAAGGCC AGCAAGCCCC CAACGGTGCG GAGGAGAAAA
    CGGCCCCATG CCCACCTGCG GGTGCCACAC CTGGAGGAGG TGATGAGCCC CGTCACCACG
    CCCACTGATG AGGACGTGGG CCACAGGATC AAACATGTGG CAGGTTCCAC ACAGACACGG
    CACATCCCCG AGGACGGCCC CAGCGGTTTC CACCTGCAGA GCGTGTCCAA GCTGCTGCTG
    GTTATCAGCT GTGTTCTGGT GCTGCTGGTC ATCCTCAACG TGATGCTCTT CTACAAACTC
    TGGATGCTGG AGTACACCAC CCAGACCCTC ACTGCCTGGC AGGGTCTCCG GCTCCAAGAA
    AGGTTACCCC AGTCCCAGAC AGAGTGGGCC CAGCTCTTAG AGTCCCAGCA AAAGTACCAC
    GATACTGAGC TCCAGAAATG GAGGGAGATC ATCAAATCCT CGGTGATGCT TCTTGACCAG
    ATGAAGGACT CACTCATCAA TCTTCAGAAC GGCATCAGGT CCCGAGACTA CACGTCAGAA
    AGCGAAGAGA AGAGGAACCG GTACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810755.1
    CDS545..2794
    Translation

    Target ORF information:

    RefSeq Version XM_010812453.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAAAGGAT TCAAGCTGTC CTGCACTGCC AGTAACTCCA ACCGCAGCAC GCCAGCCTGC 
    TCCCCCATCC TCCGGAAGCG GTCTCGCTCA CCAACCCCGC AGAACGTAGA CGGAGACACC
    ATGGTGGAGA AGGGCTCAGA TCACTCCTCG GACAAGTCGC CGTCCACTCC GGAGCAGGGC
    GTGCAGCGCA GCTGCTCCTC ACAGTCCGGC CGCAGTGGTG GCAAAAACTC CAAGTCTCAC
    AAGCGCCTCT CAAAATACGA CAGACTTTAC CTGATCAAAA AAAGCCAGAG CTGGTATAAT
    GTGTTAAGCC CCACATACAA GCAGAGAAAT GAAGACTTCA GAAAGCTCTT TAAGCAACTT
    CCAGACACGG AGCGCCTCAT TGTTGATTAC TCCTGTGCAC TCCAAAGAGA CATTCTTCTT
    CAGGGCCGAC TCTACCTCTC CGAAAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG
    GAAACTCTGC TAACTGTGCG TTTGAAAGAC ATCTGCTCCA TGACTAAAGA AAAGACAGCT
    CGCCTCATCC CCAATGCCAT CCAAGTTTGC ACTGACTCAG AAAAGCACTT TTTCACTTCG
    TTCGGGGCCC GGGATCGGAC GTACATGATG ATGTTCCGGC TCTGGCAGAA TGCTCTCCTG
    GAAAAGCCCC TGTGCCCCAA GGAGCTGTGG CACTTCGTCC ACCAGTGCTA TGGGAACGAG
    CTGGGCCTGA CCAGTGACGA CGAGGACTAC GTGCCCCCCG ATGACGACTT CAACACGATG
    GGCTACTGTG AGGAGATCCC CGTAGAAGAG AATGAAGTGA ACGACAGCTC ATCCAAAAGC
    AGCATAGAGA CCAAGCCAGA TGCCAGTCCA CAGCTGCCCA AGAAATCCAT CACCAACAGC
    ACACTGACGT CCACCGGGAG CAGTGAAGCC CCCGTCTCGT TTGACGGCCT GCCCCTGGAG
    GAGGAGGTGC TAGAGGGCGA CGGGTCTCTG GAGAAGGAGC TCGCCATCGA CAACATCATC
    GGGGAAAAGA TTGAGATCAT CGCGCCCGTG AACTCCCCTT CGCTGGACTT CAACGACAAC
    GAGGACATCC CCACTGAGCT CAGTGACTCT TCAGACACCC ACGACGAAGG GGAGGTCCAA
    GCCTTCTACG AGGACTTGAG TGGCCGGCAG TACGTGAACG AAGTCTTCAA CTTCAGCGTG
    GACAAGCTCT ATGACCTCCT GTTCACTGAC TCACCCTTCC AGCGGGACTT CATGGAGCAG
    CGGCGCTTCT CAGATATCAT CTTCCATCCG TGGAAGAAGG AAGAGAATGG AAACCAGAGC
    CGAGTGATCC TTTACACCAT CACCCTTACC AACCCTCTAG CTCCCAAAAC TGCCACCGTC
    AGGGAGACAC AGACCATGTA CAAGGCGAGC CAGGAGAGCG AGTGCTATGT GATCGACGCC
    GAGGTCCTCA CGCACGATGT ACCGTATCAC GACTACTTCT ACACCATCAA CCGCTACACG
    CTTACCCGCG TGGCCCGCAA CAAGAGTCGA CTCAGGGTCT CCACGGAGCT GCGCTATCGG
    AAACAGCCCT GGGGGCTGGT GAAAACATTC ATCGAGAAGA ACTTCTGGAG TGGGCTGGAG
    GACTACTTCC GCCACCTGGA GAGTGAGCTG ACCAAAACAG AGAGCACCTA CCTGGCGGAG
    ATGCACAGAC AGTCTCCTAA AGAGAAGGCC AGCAAGCCCC CAACGGTGCG GAGGAGAAAA
    CGGCCCCATG CCCACCTGCG GGTGCCACAC CTGGAGGAGG TGATGAGCCC CGTCACCACG
    CCCACTGATG AGGACGTGGG CCACAGGATC AAACATGTGG CAGGTTCCAC ACAGACACGG
    CACATCCCCG AGGACGGCCC CAGCGGTTTC CACCTGCAGA GCGTGTCCAA GCTGCTGCTG
    GTTATCAGCT GTGTTCTGGT GCTGCTGGTC ATCCTCAACG TGATGCTCTT CTACAAACTC
    TGGATGCTGG AGTACACCAC CCAGACCCTC ACTGCCTGGC AGGGTCTCCG GCTCCAAGAA
    AGGTTACCCC AGTCCCAGAC AGAGTGGGCC CAGCTCTTAG AGTCCCAGCA AAAGTACCAC
    GATACTGAGC TCCAGAAATG GAGGGAGATC ATCAAATCCT CGGTGATGCT TCTTGACCAG
    ATGAAGGACT CACTCATCAA TCTTCAGAAC GGCATCAGGT CCCGAGACTA CACGTCAGAA
    AGCGAAGAGA AGAGGAACCG GTACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238971
    Clone ID Related Accession (Same CDS sequence) NM_001191419.3
    Accession Version NM_001191419.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000015.1. On May 29, 2018 this sequence version replaced NM_001191419.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA GCAGAGCAGC CAGCAGAGCA GCCATGACGA TGACTCCAGC
    AGGTTCCTGA GTCCTCGCGT GCGGGAAGAA AGCACTGCCA GTAACTCCAA CCGCAGCACG
    CCAGCCTGCT CCCCCATCCT CCGGAAGCGG TCTCGCTCAC CAACCCCGCA GAACGTAGAC
    GGAGACACCA TGGTGGAGAA GGGCTCAGAT CACTCCTCGG ACAAGTCGCC GTCCACTCCG
    GAGCAGGGCG TGCAGCGCAG CTGCTCCTCA CAGTCCGGCC GCAGTGGTGG CAAAAACTCC
    AAGAAAAGCC AGAGCTGGTA TAATGTGTTA AGCCCCACAT ACAAGCAGAG AAATGAAGAC
    TTCAGAAAGC TCTTTAAGCA ACTTCCAGAC ACGGAGCGCC TCATTGTTGA TTACTCCTGT
    GCACTCCAAA GAGACATTCT TCTTCAGGGC CGACTCTACC TCTCCGAAAA CTGGATCTGC
    TTCTACAGCA ACATCTTCCG CTGGGAAACT CTGCTAACTG TGCGTTTGAA AGACATCTGC
    TCCATGACTA AAGAAAAGAC AGCTCGCCTC ATCCCCAATG CCATCCAAGT TTGCACTGAC
    TCAGAAAAGC ACTTTTTCAC TTCGTTCGGG GCCCGGGATC GGACGTACAT GATGATGTTC
    CGGCTCTGGC AGAATGCTCT CCTGGAAAAG CCCCTGTGCC CCAAGGAGCT GTGGCACTTC
    GTCCACCAGT GCTATGGGAA CGAGCTGGGC CTGACCAGTG ACGACGAGGA CTACGTGCCC
    CCCGATGACG ACTTCAACAC GATGGGCTAC TGTGAGGAGA TCCCCGTAGA AGAGAATGAA
    GTGAACGACA GCTCATCCAA AAGCAGCATA GAGACCAAGC CAGATGCCAG TCCACAGCTG
    CCCAAGAAAT CCATCACCAA CAGCACACTG ACGTCCACCG GGAGCAGTGA AGCCCCCGTC
    TCGTTTGACG GCCTGCCCCT GGAGGAGGAG GTGCTAGAGG GCGACGGGTC TCTGGAGAAG
    GAGCTCGCCA TCGACAACAT CATCGGGGAA AAGATTGAGA TCATCGCACC CGTGAACTCC
    CCTTCGCTGG ACTTCAACGA CAACGAGGAC ATCCCCACTG AGCTCAGTGA CTCTTCAGAC
    ACCCACGACG AAGGGGAGGT CCAAGCCTTC TACGAGGACT TGAGTGGCCG GCAGTACGTG
    AACGAAGTCT TCAACTTCAG CGTGGACAAG CTCTATGACC TCCTGTTCAC TGACTCACCC
    TTCCAGCGGG ACTTCATGGA GCAGCGGCGC TTCTCAGATA TCATCTTCCA TCCGTGGAAG
    AAGGAAGAGA ATGGAAACCA GAGCCGAGTG ATCCTTTACA CCATCACCCT TACCAACCCT
    CTAGCTCCCA AAACTGCCAC CGTCAGGGAG ACGCAGACCA TGTACAAGGC GAGCCAGGAG
    AGCGAGTGCT ATGTGATCGA CGCCGAGGTC CTCACGCACG ATGTACCGTA TCACGACTAC
    TTCTACACCA TCAACCGCTA CACGCTCACC CGCGTGGCCC GCAACAAGAG TCGACTCAGG
    GTCTCCACGG AGCTGCGCTA TCGGAAACAG CCCTGGGGGC TGGTGAAAAC ATTCATCGAG
    AAGAACTTCT GGAGTGGGCT GGAGGACTAC TTCCGCCACC TGGAGAGTGA GCTGACCAAA
    ACAGAGAGCA CCTACCTGGC GGAGATGCAC AGACAGTCTC CTAAAGAGAA GGCCAGCAAG
    CCCCCAACGG TGCGGAGGAG AAAACGGCCC CATGCCCACC TGCGGGTGCC ACACCTGGAG
    GAGGTGATGA GCCCCGTCAC CACGCCCACT GATGAGGACG TGGGCCACAG GATCAAACAT
    GTGGCAGGTT CCACACAGAC GCGGCACATC CCCGAGGACA GCCCCAGCGG TTTCCACCTG
    CAGAGCGTGT CCAAGCTGCT GCTGGTTATC AGCTGTGTTC TGGTGCTGCT GGTCATCCTC
    AACGTGATGC TCTTCTACAA ACTCTGGATG CTGGAGTACA CCACCCAGAC CCTCACTGCC
    TGGCAGGGTC TCCGGCTCCA AGAAAGGTTA CCCCAGTCCC AGACAGAGTG GGCCCAGCTC
    TTAGAGTCCC AGCAAAAGTA CCACGATACT GAGCTCCAGA AATGGAGGGA GATCATCAAA
    TCCTCGGTGA TGCTTCTTGA CCAGATGAAG GACTCACTCA TCAATCTTCA GAACGGCATC
    AGGTCCCGAG ACTACACGTC AGAAAGCGAA GAGAAGAGGA ACCGGTACCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178348.2
    CDS1..2634
    Translation

    Target ORF information:

    RefSeq Version NM_001191419.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191419.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA GCAGAGCAGC CAGCAGAGCA GCCATGACGA TGACTCCAGC
    AGGTTCCTGA GTCCTCGCGT GCGGGAAGAA AGCACTGCCA GTAACTCCAA CCGCAGCACG
    CCAGCCTGCT CCCCCATCCT CCGGAAGCGG TCTCGCTCAC CAACCCCGCA GAACGTAGAC
    GGAGACACCA TGGTGGAGAA GGGCTCAGAT CACTCCTCGG ACAAGTCGCC GTCCACTCCG
    GAGCAGGGCG TGCAGCGCAG CTGCTCCTCA CAGTCCGGCC GCAGTGGTGG CAAAAACTCC
    AAGAAAAGCC AGAGCTGGTA TAATGTGTTA AGCCCCACAT ACAAGCAGAG AAATGAAGAC
    TTCAGAAAGC TCTTTAAGCA ACTTCCAGAC ACGGAGCGCC TCATTGTTGA TTACTCCTGT
    GCACTCCAAA GAGACATTCT TCTTCAGGGC CGACTCTACC TCTCCGAAAA CTGGATCTGC
    TTCTACAGCA ACATCTTCCG CTGGGAAACT CTGCTAACTG TGCGTTTGAA AGACATCTGC
    TCCATGACTA AAGAAAAGAC AGCTCGCCTC ATCCCCAATG CCATCCAAGT TTGCACTGAC
    TCAGAAAAGC ACTTTTTCAC TTCGTTCGGG GCCCGGGATC GGACGTACAT GATGATGTTC
    CGGCTCTGGC AGAATGCTCT CCTGGAAAAG CCCCTGTGCC CCAAGGAGCT GTGGCACTTC
    GTCCACCAGT GCTATGGGAA CGAGCTGGGC CTGACCAGTG ACGACGAGGA CTACGTGCCC
    CCCGATGACG ACTTCAACAC GATGGGCTAC TGTGAGGAGA TCCCCGTAGA AGAGAATGAA
    GTGAACGACA GCTCATCCAA AAGCAGCATA GAGACCAAGC CAGATGCCAG TCCACAGCTG
    CCCAAGAAAT CCATCACCAA CAGCACACTG ACGTCCACCG GGAGCAGTGA AGCCCCCGTC
    TCGTTTGACG GCCTGCCCCT GGAGGAGGAG GTGCTAGAGG GCGACGGGTC TCTGGAGAAG
    GAGCTCGCCA TCGACAACAT CATCGGGGAA AAGATTGAGA TCATCGCACC CGTGAACTCC
    CCTTCGCTGG ACTTCAACGA CAACGAGGAC ATCCCCACTG AGCTCAGTGA CTCTTCAGAC
    ACCCACGACG AAGGGGAGGT CCAAGCCTTC TACGAGGACT TGAGTGGCCG GCAGTACGTG
    AACGAAGTCT TCAACTTCAG CGTGGACAAG CTCTATGACC TCCTGTTCAC TGACTCACCC
    TTCCAGCGGG ACTTCATGGA GCAGCGGCGC TTCTCAGATA TCATCTTCCA TCCGTGGAAG
    AAGGAAGAGA ATGGAAACCA GAGCCGAGTG ATCCTTTACA CCATCACCCT TACCAACCCT
    CTAGCTCCCA AAACTGCCAC CGTCAGGGAG ACGCAGACCA TGTACAAGGC GAGCCAGGAG
    AGCGAGTGCT ATGTGATCGA CGCCGAGGTC CTCACGCACG ATGTACCGTA TCACGACTAC
    TTCTACACCA TCAACCGCTA CACGCTCACC CGCGTGGCCC GCAACAAGAG TCGACTCAGG
    GTCTCCACGG AGCTGCGCTA TCGGAAACAG CCCTGGGGGC TGGTGAAAAC ATTCATCGAG
    AAGAACTTCT GGAGTGGGCT GGAGGACTAC TTCCGCCACC TGGAGAGTGA GCTGACCAAA
    ACAGAGAGCA CCTACCTGGC GGAGATGCAC AGACAGTCTC CTAAAGAGAA GGCCAGCAAG
    CCCCCAACGG TGCGGAGGAG AAAACGGCCC CATGCCCACC TGCGGGTGCC ACACCTGGAG
    GAGGTGATGA GCCCCGTCAC CACGCCCACT GATGAGGACG TGGGCCACAG GATCAAACAT
    GTGGCAGGTT CCACACAGAC GCGGCACATC CCCGAGGACA GCCCCAGCGG TTTCCACCTG
    CAGAGCGTGT CCAAGCTGCT GCTGGTTATC AGCTGTGTTC TGGTGCTGCT GGTCATCCTC
    AACGTGATGC TCTTCTACAA ACTCTGGATG CTGGAGTACA CCACCCAGAC CCTCACTGCC
    TGGCAGGGTC TCCGGCTCCA AGAAAGGTTA CCCCAGTCCC AGACAGAGTG GGCCCAGCTC
    TTAGAGTCCC AGCAAAAGTA CCACGATACT GAGCTCCAGA AATGGAGGGA GATCATCAAA
    TCCTCGGTGA TGCTTCTTGA CCAGATGAAG GACTCACTCA TCAATCTTCA GAACGGCATC
    AGGTCCCGAG ACTACACGTC AGAAAGCGAA GAGAAGAGGA ACCGGTACCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222790
    Clone ID Related Accession (Same CDS sequence) XM_010812450.3
    Accession Version XM_010812450.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA ACAGAGCAGC CAGCAGAGCA GCCAGCAGAG CAGCCATGAC
    GATGACTCCA GCAGGTTCCT GAGTCCTCGC GTGCGGGAAG AAAGCACTGC CAGTAACTCC
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGAAAAG CCAGAGCTGG TATAATGTGT TAAGCCCCAC ATACAAGCAG
    AGAAATGAAG ACTTCAGAAA GCTCTTTAAG CAACTTCCAG ACACGGAGCG CCTCATTGTT
    GATTACTCCT GTGCACTCCA AAGAGACATT CTTCTTCAGG GCCGACTCTA CCTCTCCGAA
    AACTGGATCT GCTTCTACAG CAACATCTTC CGCTGGGAAA CTCTGCTAAC TGTGCGTTTG
    AAAGACATCT GCTCCATGAC TAAAGAAAAG ACAGCTCGCC TCATCCCCAA TGCCATCCAA
    GTTTGCACTG ACTCAGAAAA GCACTTTTTC ACTTCGTTCG GGGCCCGGGA TCGGACGTAC
    ATGATGATGT TCCGGCTCTG GCAGAATGCT CTCCTGGAAA AGCCCCTGTG CCCCAAGGAG
    CTGTGGCACT TCGTCCACCA GTGCTATGGG AACGAGCTGG GCCTGACCAG TGACGACGAG
    GACTACGTGC CCCCCGATGA CGACTTCAAC ACGATGGGCT ACTGTGAGGA GATCCCCGTA
    GAAGAGAATG AAGTGAACGA CAGCTCATCC AAAAGCAGCA TAGAGACCAA GCCAGATGCC
    AGTCCACAGC TGCCCAAGAA ATCCATCACC AACAGCACAC TGACGTCCAC CGGGAGCAGT
    GAAGCCCCCG TCTCGTTTGA CGGCCTGCCC CTGGAGGAGG AGGTGCTAGA GGGCGACGGG
    TCTCTGGAGA AGGAGCTCGC CATCGACAAC ATCATCGGGG AAAAGATTGA GATCATCGCA
    CCCGTGAACT CCCCTTCGCT GGACTTCAAC GACAACGAGG ACATCCCCAC TGAGCTCAGT
    GACTCTTCAG ACACCCACGA CGAAGGGGAG GTCCAAGCCT TCTACGAGGA CTTGAGTGGC
    CGGCAGTACG TGAACGAAGT CTTCAACTTC AGCGTGGACA AGCTCTATGA CCTCCTGTTC
    ACTGACTCAC CCTTCCAGCG GGACTTCATG GAGCAGCGGC GCTTCTCAGA TATCATCTTC
    CATCCGTGGA AGAAGGAAGA GAATGGAAAC CAGAGCCGAG TGATCCTTTA CACCATCACC
    CTTACCAACC CTCTAGCTCC CAAAACTGCC ACCGTCAGGG AGACGCAGAC CATGTACAAG
    GCGAGCCAGG AGAGCGAGTG CTATGTGATC GACGCCGAGG TCCTCACGCA CGATGTACCG
    TATCACGACT ACTTCTACAC CATCAACCGC TACACGCTCA CCCGCGTGGC CCGCAACAAG
    AGTCGACTCA GGGTCTCCAC GGAGCTGCGC TATCGGAAAC AGCCCTGGGG GCTGGTGAAA
    ACATTCATCG AGAAGAACTT CTGGAGTGGG CTGGAGGACT ACTTCCGCCA CCTGGAGAGT
    GAGCTGACCA AAACAGAGAG CACCTACCTG GCGGAGATGC ACAGACAGTC TCCTAAAGAG
    AAGGCCAGCA AGCCCCCAAC GGTGCGGAGG AGAAAACGGC CCCATGCCCA CCTGCGGGTG
    CCACACCTGG AGGAGGTGAT GAGCCCCGTC ACCACGCCCA CTGATGAGGA CGTGGGCCAC
    AGGATCAAAC ATGTGGCAGG TTCCACACAG ACGCGGCACA TCCCCGAGGA CAGCCCCAGC
    GGTTTCCACC TGCAGAGCGT GTCCAAGCTG CTGCTGGTTA TCAGCTGTGT TCTGGTGCTG
    CTGGTCATCC TCAACGTGAT GCTCTTCTAC AAACTCTGGA TGCTGGAGTA CACCACCCAG
    ACCCTCACTG CCTGGCAGGG TCTCCGGCTC CAAGAAAGGT TACCCCAGTC CCAGACAGAG
    TGGGCCCAGC TCTTAGAGTC CCAGCAAAAG TACCACGATA CTGAGCTCCA GAAATGGAGG
    GAGATCATCA AATCCTCGGT GATGCTTCTT GACCAGATGA AGGACTCACT CATCAATCTT
    CAGAACGGCA TCAGGTCCCG AGACTACACG TCAGAAAGCG AAGAGAAGAG GAACCGGTAC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810752.2
    CDS27..2672
    Translation

    Target ORF information:

    RefSeq Version XM_010812450.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812450.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA ACAGAGCAGC CAGCAGAGCA GCCAGCAGAG CAGCCATGAC
    GATGACTCCA GCAGGTTCCT GAGTCCTCGC GTGCGGGAAG AAAGCACTGC CAGTAACTCC
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGAAAAG CCAGAGCTGG TATAATGTGT TAAGCCCCAC ATACAAGCAG
    AGAAATGAAG ACTTCAGAAA GCTCTTTAAG CAACTTCCAG ACACGGAGCG CCTCATTGTT
    GATTACTCCT GTGCACTCCA AAGAGACATT CTTCTTCAGG GCCGACTCTA CCTCTCCGAA
    AACTGGATCT GCTTCTACAG CAACATCTTC CGCTGGGAAA CTCTGCTAAC TGTGCGTTTG
    AAAGACATCT GCTCCATGAC TAAAGAAAAG ACAGCTCGCC TCATCCCCAA TGCCATCCAA
    GTTTGCACTG ACTCAGAAAA GCACTTTTTC ACTTCGTTCG GGGCCCGGGA TCGGACGTAC
    ATGATGATGT TCCGGCTCTG GCAGAATGCT CTCCTGGAAA AGCCCCTGTG CCCCAAGGAG
    CTGTGGCACT TCGTCCACCA GTGCTATGGG AACGAGCTGG GCCTGACCAG TGACGACGAG
    GACTACGTGC CCCCCGATGA CGACTTCAAC ACGATGGGCT ACTGTGAGGA GATCCCCGTA
    GAAGAGAATG AAGTGAACGA CAGCTCATCC AAAAGCAGCA TAGAGACCAA GCCAGATGCC
    AGTCCACAGC TGCCCAAGAA ATCCATCACC AACAGCACAC TGACGTCCAC CGGGAGCAGT
    GAAGCCCCCG TCTCGTTTGA CGGCCTGCCC CTGGAGGAGG AGGTGCTAGA GGGCGACGGG
    TCTCTGGAGA AGGAGCTCGC CATCGACAAC ATCATCGGGG AAAAGATTGA GATCATCGCA
    CCCGTGAACT CCCCTTCGCT GGACTTCAAC GACAACGAGG ACATCCCCAC TGAGCTCAGT
    GACTCTTCAG ACACCCACGA CGAAGGGGAG GTCCAAGCCT TCTACGAGGA CTTGAGTGGC
    CGGCAGTACG TGAACGAAGT CTTCAACTTC AGCGTGGACA AGCTCTATGA CCTCCTGTTC
    ACTGACTCAC CCTTCCAGCG GGACTTCATG GAGCAGCGGC GCTTCTCAGA TATCATCTTC
    CATCCGTGGA AGAAGGAAGA GAATGGAAAC CAGAGCCGAG TGATCCTTTA CACCATCACC
    CTTACCAACC CTCTAGCTCC CAAAACTGCC ACCGTCAGGG AGACGCAGAC CATGTACAAG
    GCGAGCCAGG AGAGCGAGTG CTATGTGATC GACGCCGAGG TCCTCACGCA CGATGTACCG
    TATCACGACT ACTTCTACAC CATCAACCGC TACACGCTCA CCCGCGTGGC CCGCAACAAG
    AGTCGACTCA GGGTCTCCAC GGAGCTGCGC TATCGGAAAC AGCCCTGGGG GCTGGTGAAA
    ACATTCATCG AGAAGAACTT CTGGAGTGGG CTGGAGGACT ACTTCCGCCA CCTGGAGAGT
    GAGCTGACCA AAACAGAGAG CACCTACCTG GCGGAGATGC ACAGACAGTC TCCTAAAGAG
    AAGGCCAGCA AGCCCCCAAC GGTGCGGAGG AGAAAACGGC CCCATGCCCA CCTGCGGGTG
    CCACACCTGG AGGAGGTGAT GAGCCCCGTC ACCACGCCCA CTGATGAGGA CGTGGGCCAC
    AGGATCAAAC ATGTGGCAGG TTCCACACAG ACGCGGCACA TCCCCGAGGA CAGCCCCAGC
    GGTTTCCACC TGCAGAGCGT GTCCAAGCTG CTGCTGGTTA TCAGCTGTGT TCTGGTGCTG
    CTGGTCATCC TCAACGTGAT GCTCTTCTAC AAACTCTGGA TGCTGGAGTA CACCACCCAG
    ACCCTCACTG CCTGGCAGGG TCTCCGGCTC CAAGAAAGGT TACCCCAGTC CCAGACAGAG
    TGGGCCCAGC TCTTAGAGTC CCAGCAAAAG TACCACGATA CTGAGCTCCA GAAATGGAGG
    GAGATCATCA AATCCTCGGT GATGCTTCTT GACCAGATGA AGGACTCACT CATCAATCTT
    CAGAACGGCA TCAGGTCCCG AGACTACACG TCAGAAAGCG AAGAGAAGAG GAACCGGTAC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222791
    Clone ID Related Accession (Same CDS sequence) XM_024975268.1
    Accession Version XM_024975268.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCCAACA GAGCAGCCAG CAGAGCAGCC AGCAGAGCAG CCATGACGAT
    GACTCCAGCA GGTTCCTGAG TCCTCGCGTG CGGGAAGAAA GCACTGCCAG TAACTCCAAC
    CGCAGCACGC CAGCCTGCTC CCCCATCCTC CGGAAGCGGT CTCGCTCACC AACCCCGCAG
    AACGTAGACG GAGACACCAT GGTGGAGAAG GGCTCAGATC ACTCCTCGGA CAAGTCGCCG
    TCCACTCCGG AGCAGGGCGT GCAGCGCAGC TGCTCCTCAC AGTCCGGCCG CAGTGGTGGC
    AAAAACTCCA AGAAAAGCCA GAGCTGGTAT AATGTGTTAA GCCCCACATA CAAGCAGAGA
    AATGAAGACT TCAGAAAGCT CTTTAAGCAA CTTCCAGACA CGGAGCGCCT CATTGTTGAT
    TACTCCTGTG CACTCCAAAG AGACATTCTT CTTCAGGGCC GACTCTACCT CTCCGAAAAC
    TGGATCTGCT TCTACAGCAA CATCTTCCGC TGGGAAACTC TGCTAACTGT GCGTTTGAAA
    GACATCTGCT CCATGACTAA AGAAAAGACA GCTCGCCTCA TCCCCAATGC CATCCAAGTT
    TGCACTGACT CAGAAAAGCA CTTTTTCACT TCGTTCGGGG CCCGGGATCG GACGTACATG
    ATGATGTTCC GGCTCTGGCA GAATGCTCTC CTGGAAAAGC CCCTGTGCCC CAAGGAGCTG
    TGGCACTTCG TCCACCAGTG CTATGGGAAC GAGCTGGGCC TGACCAGTGA CGACGAGGAC
    TACGTGCCCC CCGATGACGA CTTCAACACG ATGGGCTACT GTGAGGAGAT CCCCGTAGAA
    GAGAATGAAG TGAACGACAG CTCATCCAAA AGCAGCATAG AGACCAAGCC AGATGCCAGT
    CCACAGCTGC CCAAGAAATC CATCACCAAC AGCACACTGA CGTCCACCGG GAGCAGTGAA
    GCCCCCGTCT CGTTTGACGG CCTGCCCCTG GAGGAGGAGG TGCTAGAGGG CGACGGGTCT
    CTGGAGAAGG AGCTCGCCAT CGACAACATC ATCGGGGAAA AGATTGAGAT CATCGCACCC
    GTGAACTCCC CTTCGCTGGA CTTCAACGAC AACGAGGACA TCCCCACTGA GCTCAGTGAC
    TCTTCAGACA CCCACGACGA AGGGGAGGTC CAAGCCTTCT ACGAGGACTT GAGTGGCCGG
    CAGTACGTGA ACGAAGTCTT CAACTTCAGC GTGGACAAGC TCTATGACCT CCTGTTCACT
    GACTCACCCT TCCAGCGGGA CTTCATGGAG CAGCGGCGCT TCTCAGATAT CATCTTCCAT
    CCGTGGAAGA AGGAAGAGAA TGGAAACCAG AGCCGAGTGA TCCTTTACAC CATCACCCTT
    ACCAACCCTC TAGCTCCCAA AACTGCCACC GTCAGGGAGA CGCAGACCAT GTACAAGGCG
    AGCCAGGAGA GCGAGTGCTA TGTGATCGAC GCCGAGGTCC TCACGCACGA TGTACCGTAT
    CACGACTACT TCTACACCAT CAACCGCTAC ACGCTCACCC GCGTGGCCCG CAACAAGAGT
    CGACTCAGGG TCTCCACGGA GCTGCGCTAT CGGAAACAGC CCTGGGGGCT GGTGAAAACA
    TTCATCGAGA AGAACTTCTG GAGTGGGCTG GAGGACTACT TCCGCCACCT GGAGAGTGAG
    CTGACCAAAA CAGAGAGCAC CTACCTGGCG GAGATGCACA GACAGTCTCC TAAAGAGAAG
    GCCAGCAAGC CCCCAACGGT GCGGAGGAGA AAACGGCCCC ATGCCCACCT GCGGGTGCCA
    CACCTGGAGG AGGTGATGAG CCCCGTCACC ACGCCCACTG ATGAGGACGT GGGCCACAGG
    ATCAAACATG TGGCAGGTTC CACACAGACG CGGCACATCC CCGAGGACAG CCCCAGCGGT
    TTCCACCTGC AGAGCGTGTC CAAGCTGCTG CTGGTTATCA GCTGTGTTCT GGTGCTGCTG
    GTCATCCTCA ACGTGATGCT CTTCTACAAA CTCTGGATGC TGGAGTACAC CACCCAGACC
    CTCACTGCCT GGCAGGGTCT CCGGCTCCAA GAAAGGTTAC CCCAGTCCCA GACAGAGTGG
    GCCCAGCTCT TAGAGTCCCA GCAAAAGTAC CACGATACTG AGCTCCAGAA ATGGAGGGAG
    ATCATCAAAT CCTCGGTGAT GCTTCTTGAC CAGATGAAGG ACTCACTCAT CAATCTTCAG
    AACGGCATCA GGTCCCGAGA CTACACGTCA GAAAGCGAAG AGAAGAGGAA CCGGTACCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831036.1
    CDS27..2669
    Translation

    Target ORF information:

    RefSeq Version XM_024975268.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975268.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCCAACA GAGCAGCCAG CAGAGCAGCC AGCAGAGCAG CCATGACGAT
    GACTCCAGCA GGTTCCTGAG TCCTCGCGTG CGGGAAGAAA GCACTGCCAG TAACTCCAAC
    CGCAGCACGC CAGCCTGCTC CCCCATCCTC CGGAAGCGGT CTCGCTCACC AACCCCGCAG
    AACGTAGACG GAGACACCAT GGTGGAGAAG GGCTCAGATC ACTCCTCGGA CAAGTCGCCG
    TCCACTCCGG AGCAGGGCGT GCAGCGCAGC TGCTCCTCAC AGTCCGGCCG CAGTGGTGGC
    AAAAACTCCA AGAAAAGCCA GAGCTGGTAT AATGTGTTAA GCCCCACATA CAAGCAGAGA
    AATGAAGACT TCAGAAAGCT CTTTAAGCAA CTTCCAGACA CGGAGCGCCT CATTGTTGAT
    TACTCCTGTG CACTCCAAAG AGACATTCTT CTTCAGGGCC GACTCTACCT CTCCGAAAAC
    TGGATCTGCT TCTACAGCAA CATCTTCCGC TGGGAAACTC TGCTAACTGT GCGTTTGAAA
    GACATCTGCT CCATGACTAA AGAAAAGACA GCTCGCCTCA TCCCCAATGC CATCCAAGTT
    TGCACTGACT CAGAAAAGCA CTTTTTCACT TCGTTCGGGG CCCGGGATCG GACGTACATG
    ATGATGTTCC GGCTCTGGCA GAATGCTCTC CTGGAAAAGC CCCTGTGCCC CAAGGAGCTG
    TGGCACTTCG TCCACCAGTG CTATGGGAAC GAGCTGGGCC TGACCAGTGA CGACGAGGAC
    TACGTGCCCC CCGATGACGA CTTCAACACG ATGGGCTACT GTGAGGAGAT CCCCGTAGAA
    GAGAATGAAG TGAACGACAG CTCATCCAAA AGCAGCATAG AGACCAAGCC AGATGCCAGT
    CCACAGCTGC CCAAGAAATC CATCACCAAC AGCACACTGA CGTCCACCGG GAGCAGTGAA
    GCCCCCGTCT CGTTTGACGG CCTGCCCCTG GAGGAGGAGG TGCTAGAGGG CGACGGGTCT
    CTGGAGAAGG AGCTCGCCAT CGACAACATC ATCGGGGAAA AGATTGAGAT CATCGCACCC
    GTGAACTCCC CTTCGCTGGA CTTCAACGAC AACGAGGACA TCCCCACTGA GCTCAGTGAC
    TCTTCAGACA CCCACGACGA AGGGGAGGTC CAAGCCTTCT ACGAGGACTT GAGTGGCCGG
    CAGTACGTGA ACGAAGTCTT CAACTTCAGC GTGGACAAGC TCTATGACCT CCTGTTCACT
    GACTCACCCT TCCAGCGGGA CTTCATGGAG CAGCGGCGCT TCTCAGATAT CATCTTCCAT
    CCGTGGAAGA AGGAAGAGAA TGGAAACCAG AGCCGAGTGA TCCTTTACAC CATCACCCTT
    ACCAACCCTC TAGCTCCCAA AACTGCCACC GTCAGGGAGA CGCAGACCAT GTACAAGGCG
    AGCCAGGAGA GCGAGTGCTA TGTGATCGAC GCCGAGGTCC TCACGCACGA TGTACCGTAT
    CACGACTACT TCTACACCAT CAACCGCTAC ACGCTCACCC GCGTGGCCCG CAACAAGAGT
    CGACTCAGGG TCTCCACGGA GCTGCGCTAT CGGAAACAGC CCTGGGGGCT GGTGAAAACA
    TTCATCGAGA AGAACTTCTG GAGTGGGCTG GAGGACTACT TCCGCCACCT GGAGAGTGAG
    CTGACCAAAA CAGAGAGCAC CTACCTGGCG GAGATGCACA GACAGTCTCC TAAAGAGAAG
    GCCAGCAAGC CCCCAACGGT GCGGAGGAGA AAACGGCCCC ATGCCCACCT GCGGGTGCCA
    CACCTGGAGG AGGTGATGAG CCCCGTCACC ACGCCCACTG ATGAGGACGT GGGCCACAGG
    ATCAAACATG TGGCAGGTTC CACACAGACG CGGCACATCC CCGAGGACAG CCCCAGCGGT
    TTCCACCTGC AGAGCGTGTC CAAGCTGCTG CTGGTTATCA GCTGTGTTCT GGTGCTGCTG
    GTCATCCTCA ACGTGATGCT CTTCTACAAA CTCTGGATGC TGGAGTACAC CACCCAGACC
    CTCACTGCCT GGCAGGGTCT CCGGCTCCAA GAAAGGTTAC CCCAGTCCCA GACAGAGTGG
    GCCCAGCTCT TAGAGTCCCA GCAAAAGTAC CACGATACTG AGCTCCAGAA ATGGAGGGAG
    ATCATCAAAT CCTCGGTGAT GCTTCTTGAC CAGATGAAGG ACTCACTCAT CAATCTTCAG
    AACGGCATCA GGTCCCGAGA CTACACGTCA GAAAGCGAAG AGAAGAGGAA CCGGTACCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222792
    Clone ID Related Accession (Same CDS sequence) XM_024975269.1
    Accession Version XM_024975269.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTTTTGG TGCTAGGGAG ACGATGCAGA GAGAAAACTC TTAGCAAAAT GAGAAGAACT 
    TGTAAGAAGG AGACAAGCAG CCAACAGAGC AGCCAGCAGA GCAGCCAGCA GAGCAGCCAT
    GACGATGACT CCAGCAGGTT CCTGAGTCCT CGCGTGCGGG AAGAAAGCAC TGCCAGTAAC
    TCCAACCGCA GCACGCCAGC CTGCTCCCCC ATCCTCCGGA AGCGGTCTCG CTCACCAACC
    CCGCAGAACG TAGACGGAGA CACCATGGTG GAGAAGGGCT CAGATCACTC CTCGGACAAG
    TCGCCGTCCA CTCCGGAGCA GGGCGTGCAG CGCAGCTGCT CCTCACAGTC CGGCCGCAGT
    GGTGGCAAAA ACTCCAAGAA AAGCCAGAGC TGGTATAATG TGTTAAGCCC CACATACAAG
    CAGAGAAATG AAGACTTCAG AAAGCTCTTT AAGCAACTTC CAGACACGGA GCGCCTCATT
    GTTGATTACT CCTGTGCACT CCAAAGAGAC ATTCTTCTTC AGGGCCGACT CTACCTCTCC
    GAAAACTGGA TCTGCTTCTA CAGCAACATC TTCCGCTGGG AAACTCTGCT AACTGTGCGT
    TTGAAAGACA TCTGCTCCAT GACTAAAGAA AAGACAGCTC GCCTCATCCC CAATGCCATC
    CAAGTTTGCA CTGACTCAGA AAAGCACTTT TTCACTTCGT TCGGGGCCCG GGATCGGACG
    TACATGATGA TGTTCCGGCT CTGGCAGAAT GCTCTCCTGG AAAAGCCCCT GTGCCCCAAG
    GAGCTGTGGC ACTTCGTCCA CCAGTGCTAT GGGAACGAGC TGGGCCTGAC CAGTGACGAC
    GAGGACTACG TGCCCCCCGA TGACGACTTC AACACGATGG GCTACTGTGA GGAGATCCCC
    GTAGAAGAGA ATGAAGTGAA CGACAGCTCA TCCAAAAGCA GCATAGAGAC CAAGCCAGAT
    GCCAGTCCAC AGCTGCCCAA GAAATCCATC ACCAACAGCA CACTGACGTC CACCGGGAGC
    AGTGAAGCCC CCGTCTCGTT TGACGGCCTG CCCCTGGAGG AGGAGGTGCT AGAGGGCGAC
    GGGTCTCTGG AGAAGGAGCT CGCCATCGAC AACATCATCG GGGAAAAGAT TGAGATCATC
    GCACCCGTGA ACTCCCCTTC GCTGGACTTC AACGACAACG AGGACATCCC CACTGAGCTC
    AGTGACTCTT CAGACACCCA CGACGAAGGG GAGGTCCAAG CCTTCTACGA GGACTTGAGT
    GGCCGGCAGT ACGTGAACGA AGTCTTCAAC TTCAGCGTGG ACAAGCTCTA TGACCTCCTG
    TTCACTGACT CACCCTTCCA GCGGGACTTC ATGGAGCAGC GGCGCTTCTC AGATATCATC
    TTCCATCCGT GGAAGAAGGA AGAGAATGGA AACCAGAGCC GAGTGATCCT TTACACCATC
    ACCCTTACCA ACCCTCTAGC TCCCAAAACT GCCACCGTCA GGGAGACGCA GACCATGTAC
    AAGGCGAGCC AGGAGAGCGA GTGCTATGTG ATCGACGCCG AGGTCCTCAC GCACGATGTA
    CCGTATCACG ACTACTTCTA CACCATCAAC CGCTACACGC TCACCCGCGT GGCCCGCAAC
    AAGAGTCGAC TCAGGGTCTC CACGGAGCTG CGCTATCGGA AACAGCCCTG GGGGCTGGTG
    AAAACATTCA TCGAGAAGAA CTTCTGGAGT GGGCTGGAGG ACTACTTCCG CCACCTGGAG
    AGTGAGCTGA CCAAAACAGA GAGCACCTAC CTGGCGGAGA TGCACAGACA GTCTCCTAAA
    GAGAAGGCCA GCAAGCCCCC AACGGTGCGG AGGAGAAAAC GGCCCCATGC CCACCTGCGG
    GTGCCACACC TGGAGGAGGT GATGAGCCCC GTCACCACGC CCACTGATGA GGACGTGGGC
    CACAGGATCA AACATGTGGC AGGTTCCACA CAGACGCGGC ACATCCCCGA GGACAGCCCC
    AGCGGTTTCC ACCTGCAGAG CGTGTCCAAG CTGCTGCTGG TTATCAGCTG TGTTCTGGTG
    CTGCTGGTCA TCCTCAACGT GATGCTCTTC TACAAACTCT GGATGCTGGA GTACACCACC
    CAGACCCTCA CTGCCTGGCA GGGTCTCCGG CTCCAAGAAA GGTTACCCCA GTCCCAGACA
    GAGTGGGCCC AGCTCTTAGA GTCCCAGCAA AAGTACCACG ATACTGAGCT CCAGAAATGG
    AGGGAGATCA TCAAATCCTC GGTGATGCTT CTTGACCAGA TGAAGGACTC ACTCATCAAT
    CTTCAGAACG GCATCAGGTC CCGAGACTAC ACGTCAGAAA GCGAAGAGAA GAGGAACCGG
    TACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831037.1
    CDS275..2623
    Translation

    Target ORF information:

    RefSeq Version XM_024975269.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975269.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTTTTGG TGCTAGGGAG ACGATGCAGA GAGAAAACTC TTAGCAAAAT GAGAAGAACT 
    TGTAAGAAGG AGACAAGCAG CCAACAGAGC AGCCAGCAGA GCAGCCAGCA GAGCAGCCAT
    GACGATGACT CCAGCAGGTT CCTGAGTCCT CGCGTGCGGG AAGAAAGCAC TGCCAGTAAC
    TCCAACCGCA GCACGCCAGC CTGCTCCCCC ATCCTCCGGA AGCGGTCTCG CTCACCAACC
    CCGCAGAACG TAGACGGAGA CACCATGGTG GAGAAGGGCT CAGATCACTC CTCGGACAAG
    TCGCCGTCCA CTCCGGAGCA GGGCGTGCAG CGCAGCTGCT CCTCACAGTC CGGCCGCAGT
    GGTGGCAAAA ACTCCAAGAA AAGCCAGAGC TGGTATAATG TGTTAAGCCC CACATACAAG
    CAGAGAAATG AAGACTTCAG AAAGCTCTTT AAGCAACTTC CAGACACGGA GCGCCTCATT
    GTTGATTACT CCTGTGCACT CCAAAGAGAC ATTCTTCTTC AGGGCCGACT CTACCTCTCC
    GAAAACTGGA TCTGCTTCTA CAGCAACATC TTCCGCTGGG AAACTCTGCT AACTGTGCGT
    TTGAAAGACA TCTGCTCCAT GACTAAAGAA AAGACAGCTC GCCTCATCCC CAATGCCATC
    CAAGTTTGCA CTGACTCAGA AAAGCACTTT TTCACTTCGT TCGGGGCCCG GGATCGGACG
    TACATGATGA TGTTCCGGCT CTGGCAGAAT GCTCTCCTGG AAAAGCCCCT GTGCCCCAAG
    GAGCTGTGGC ACTTCGTCCA CCAGTGCTAT GGGAACGAGC TGGGCCTGAC CAGTGACGAC
    GAGGACTACG TGCCCCCCGA TGACGACTTC AACACGATGG GCTACTGTGA GGAGATCCCC
    GTAGAAGAGA ATGAAGTGAA CGACAGCTCA TCCAAAAGCA GCATAGAGAC CAAGCCAGAT
    GCCAGTCCAC AGCTGCCCAA GAAATCCATC ACCAACAGCA CACTGACGTC CACCGGGAGC
    AGTGAAGCCC CCGTCTCGTT TGACGGCCTG CCCCTGGAGG AGGAGGTGCT AGAGGGCGAC
    GGGTCTCTGG AGAAGGAGCT CGCCATCGAC AACATCATCG GGGAAAAGAT TGAGATCATC
    GCACCCGTGA ACTCCCCTTC GCTGGACTTC AACGACAACG AGGACATCCC CACTGAGCTC
    AGTGACTCTT CAGACACCCA CGACGAAGGG GAGGTCCAAG CCTTCTACGA GGACTTGAGT
    GGCCGGCAGT ACGTGAACGA AGTCTTCAAC TTCAGCGTGG ACAAGCTCTA TGACCTCCTG
    TTCACTGACT CACCCTTCCA GCGGGACTTC ATGGAGCAGC GGCGCTTCTC AGATATCATC
    TTCCATCCGT GGAAGAAGGA AGAGAATGGA AACCAGAGCC GAGTGATCCT TTACACCATC
    ACCCTTACCA ACCCTCTAGC TCCCAAAACT GCCACCGTCA GGGAGACGCA GACCATGTAC
    AAGGCGAGCC AGGAGAGCGA GTGCTATGTG ATCGACGCCG AGGTCCTCAC GCACGATGTA
    CCGTATCACG ACTACTTCTA CACCATCAAC CGCTACACGC TCACCCGCGT GGCCCGCAAC
    AAGAGTCGAC TCAGGGTCTC CACGGAGCTG CGCTATCGGA AACAGCCCTG GGGGCTGGTG
    AAAACATTCA TCGAGAAGAA CTTCTGGAGT GGGCTGGAGG ACTACTTCCG CCACCTGGAG
    AGTGAGCTGA CCAAAACAGA GAGCACCTAC CTGGCGGAGA TGCACAGACA GTCTCCTAAA
    GAGAAGGCCA GCAAGCCCCC AACGGTGCGG AGGAGAAAAC GGCCCCATGC CCACCTGCGG
    GTGCCACACC TGGAGGAGGT GATGAGCCCC GTCACCACGC CCACTGATGA GGACGTGGGC
    CACAGGATCA AACATGTGGC AGGTTCCACA CAGACGCGGC ACATCCCCGA GGACAGCCCC
    AGCGGTTTCC ACCTGCAGAG CGTGTCCAAG CTGCTGCTGG TTATCAGCTG TGTTCTGGTG
    CTGCTGGTCA TCCTCAACGT GATGCTCTTC TACAAACTCT GGATGCTGGA GTACACCACC
    CAGACCCTCA CTGCCTGGCA GGGTCTCCGG CTCCAAGAAA GGTTACCCCA GTCCCAGACA
    GAGTGGGCCC AGCTCTTAGA GTCCCAGCAA AAGTACCACG ATACTGAGCT CCAGAAATGG
    AGGGAGATCA TCAAATCCTC GGTGATGCTT CTTGACCAGA TGAAGGACTC ACTCATCAAT
    CTTCAGAACG GCATCAGGTC CCGAGACTAC ACGTCAGAAA GCGAAGAGAA GAGGAACCGG
    TACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222793
    Clone ID Related Accession (Same CDS sequence) XM_005215954.3
    Accession Version XM_005215954.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215954.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTGGGAAC ACACTGAATC AGAGTATCTG CATGAGGCCC ACGGGCTGAA GCAAGGCTGC 
    TTCTCTTTAA TGGTTAAGGG TGGATATCCT GGAGGTGACC CACTGGCCTT GACTCTGGGG
    CCCACCAGCT ATTATCTCTG TGATCTTGGC ACTGCCAGTA ACTCCAACCG CAGCACGCCA
    GCCTGCTCCC CCATCCTCCG GAAGCGGTCT CGCTCACCAA CCCCGCAGAA CGTAGACGGA
    GACACCATGG TGGAGAAGGG CTCAGATCAC TCCTCGGACA AGTCGCCGTC CACTCCGGAG
    CAGGGCGTGC AGCGCAGCTG CTCCTCACAG TCCGGCCGCA GTGGTGGCAA AAACTCCAAG
    AAAAGCCAGA GCTGGTATAA TGTGTTAAGC CCCACATACA AGCAGAGAAA TGAAGACTTC
    AGAAAGCTCT TTAAGCAACT TCCAGACACG GAGCGCCTCA TTGTTGATTA CTCCTGTGCA
    CTCCAAAGAG ACATTCTTCT TCAGGGCCGA CTCTACCTCT CCGAAAACTG GATCTGCTTC
    TACAGCAACA TCTTCCGCTG GGAAACTCTG CTAACTGTGC GTTTGAAAGA CATCTGCTCC
    ATGACTAAAG AAAAGACAGC TCGCCTCATC CCCAATGCCA TCCAAGTTTG CACTGACTCA
    GAAAAGCACT TTTTCACTTC GTTCGGGGCC CGGGATCGGA CGTACATGAT GATGTTCCGG
    CTCTGGCAGA ATGCTCTCCT GGAAAAGCCC CTGTGCCCCA AGGAGCTGTG GCACTTCGTC
    CACCAGTGCT ATGGGAACGA GCTGGGCCTG ACCAGTGACG ACGAGGACTA CGTGCCCCCC
    GATGACGACT TCAACACGAT GGGCTACTGT GAGGAGATCC CCGTAGAAGA GAATGAAGTG
    AACGACAGCT CATCCAAAAG CAGCATAGAG ACCAAGCCAG ATGCCAGTCC ACAGCTGCCC
    AAGAAATCCA TCACCAACAG CACACTGACG TCCACCGGGA GCAGTGAAGC CCCCGTCTCG
    TTTGACGGCC TGCCCCTGGA GGAGGAGGTG CTAGAGGGCG ACGGGTCTCT GGAGAAGGAG
    CTCGCCATCG ACAACATCAT CGGGGAAAAG ATTGAGATCA TCGCACCCGT GAACTCCCCT
    TCGCTGGACT TCAACGACAA CGAGGACATC CCCACTGAGC TCAGTGACTC TTCAGACACC
    CACGACGAAG GGGAGGTCCA AGCCTTCTAC GAGGACTTGA GTGGCCGGCA GTACGTGAAC
    GAAGTCTTCA ACTTCAGCGT GGACAAGCTC TATGACCTCC TGTTCACTGA CTCACCCTTC
    CAGCGGGACT TCATGGAGCA GCGGCGCTTC TCAGATATCA TCTTCCATCC GTGGAAGAAG
    GAAGAGAATG GAAACCAGAG CCGAGTGATC CTTTACACCA TCACCCTTAC CAACCCTCTA
    GCTCCCAAAA CTGCCACCGT CAGGGAGACG CAGACCATGT ACAAGGCGAG CCAGGAGAGC
    GAGTGCTATG TGATCGACGC CGAGGTCCTC ACGCACGATG TACCGTATCA CGACTACTTC
    TACACCATCA ACCGCTACAC GCTCACCCGC GTGGCCCGCA ACAAGAGTCG ACTCAGGGTC
    TCCACGGAGC TGCGCTATCG GAAACAGCCC TGGGGGCTGG TGAAAACATT CATCGAGAAG
    AACTTCTGGA GTGGGCTGGA GGACTACTTC CGCCACCTGG AGAGTGAGCT GACCAAAACA
    GAGAGCACCT ACCTGGCGGA GATGCACAGA CAGTCTCCTA AAGAGAAGGC CAGCAAGCCC
    CCAACGGTGC GGAGGAGAAA ACGGCCCCAT GCCCACCTGC GGGTGCCACA CCTGGAGGAG
    GTGATGAGCC CCGTCACCAC GCCCACTGAT GAGGACGTGG GCCACAGGAT CAAACATGTG
    GCAGGTTCCA CACAGACGCG GCACATCCCC GAGGACAGCC CCAGCGGTTT CCACCTGCAG
    AGCGTGTCCA AGCTGCTGCT GGTTATCAGC TGTGTTCTGG TGCTGCTGGT CATCCTCAAC
    GTGATGCTCT TCTACAAACT CTGGATGCTG GAGTACACCA CCCAGACCCT CACTGCCTGG
    CAGGGTCTCC GGCTCCAAGA AAGGTTACCC CAGTCCCAGA CAGAGTGGGC CCAGCTCTTA
    GAGTCCCAGC AAAAGTACCA CGATACTGAG CTCCAGAAAT GGAGGGAGAT CATCAAATCC
    TCGGTGATGC TTCTTGACCA GATGAAGGAC TCACTCATCA ATCTTCAGAA CGGCATCAGG
    TCCCGAGACT ACACGTCAGA AAGCGAAGAG AAGAGGAACC GGTACCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216011.2
    CDS45..2375
    Translation

    Target ORF information:

    RefSeq Version XM_005215954.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215954.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTGGGAAC ACACTGAATC AGAGTATCTG CATGAGGCCC ACGGGCTGAA GCAAGGCTGC 
    TTCTCTTTAA TGGTTAAGGG TGGATATCCT GGAGGTGACC CACTGGCCTT GACTCTGGGG
    CCCACCAGCT ATTATCTCTG TGATCTTGGC ACTGCCAGTA ACTCCAACCG CAGCACGCCA
    GCCTGCTCCC CCATCCTCCG GAAGCGGTCT CGCTCACCAA CCCCGCAGAA CGTAGACGGA
    GACACCATGG TGGAGAAGGG CTCAGATCAC TCCTCGGACA AGTCGCCGTC CACTCCGGAG
    CAGGGCGTGC AGCGCAGCTG CTCCTCACAG TCCGGCCGCA GTGGTGGCAA AAACTCCAAG
    AAAAGCCAGA GCTGGTATAA TGTGTTAAGC CCCACATACA AGCAGAGAAA TGAAGACTTC
    AGAAAGCTCT TTAAGCAACT TCCAGACACG GAGCGCCTCA TTGTTGATTA CTCCTGTGCA
    CTCCAAAGAG ACATTCTTCT TCAGGGCCGA CTCTACCTCT CCGAAAACTG GATCTGCTTC
    TACAGCAACA TCTTCCGCTG GGAAACTCTG CTAACTGTGC GTTTGAAAGA CATCTGCTCC
    ATGACTAAAG AAAAGACAGC TCGCCTCATC CCCAATGCCA TCCAAGTTTG CACTGACTCA
    GAAAAGCACT TTTTCACTTC GTTCGGGGCC CGGGATCGGA CGTACATGAT GATGTTCCGG
    CTCTGGCAGA ATGCTCTCCT GGAAAAGCCC CTGTGCCCCA AGGAGCTGTG GCACTTCGTC
    CACCAGTGCT ATGGGAACGA GCTGGGCCTG ACCAGTGACG ACGAGGACTA CGTGCCCCCC
    GATGACGACT TCAACACGAT GGGCTACTGT GAGGAGATCC CCGTAGAAGA GAATGAAGTG
    AACGACAGCT CATCCAAAAG CAGCATAGAG ACCAAGCCAG ATGCCAGTCC ACAGCTGCCC
    AAGAAATCCA TCACCAACAG CACACTGACG TCCACCGGGA GCAGTGAAGC CCCCGTCTCG
    TTTGACGGCC TGCCCCTGGA GGAGGAGGTG CTAGAGGGCG ACGGGTCTCT GGAGAAGGAG
    CTCGCCATCG ACAACATCAT CGGGGAAAAG ATTGAGATCA TCGCACCCGT GAACTCCCCT
    TCGCTGGACT TCAACGACAA CGAGGACATC CCCACTGAGC TCAGTGACTC TTCAGACACC
    CACGACGAAG GGGAGGTCCA AGCCTTCTAC GAGGACTTGA GTGGCCGGCA GTACGTGAAC
    GAAGTCTTCA ACTTCAGCGT GGACAAGCTC TATGACCTCC TGTTCACTGA CTCACCCTTC
    CAGCGGGACT TCATGGAGCA GCGGCGCTTC TCAGATATCA TCTTCCATCC GTGGAAGAAG
    GAAGAGAATG GAAACCAGAG CCGAGTGATC CTTTACACCA TCACCCTTAC CAACCCTCTA
    GCTCCCAAAA CTGCCACCGT CAGGGAGACG CAGACCATGT ACAAGGCGAG CCAGGAGAGC
    GAGTGCTATG TGATCGACGC CGAGGTCCTC ACGCACGATG TACCGTATCA CGACTACTTC
    TACACCATCA ACCGCTACAC GCTCACCCGC GTGGCCCGCA ACAAGAGTCG ACTCAGGGTC
    TCCACGGAGC TGCGCTATCG GAAACAGCCC TGGGGGCTGG TGAAAACATT CATCGAGAAG
    AACTTCTGGA GTGGGCTGGA GGACTACTTC CGCCACCTGG AGAGTGAGCT GACCAAAACA
    GAGAGCACCT ACCTGGCGGA GATGCACAGA CAGTCTCCTA AAGAGAAGGC CAGCAAGCCC
    CCAACGGTGC GGAGGAGAAA ACGGCCCCAT GCCCACCTGC GGGTGCCACA CCTGGAGGAG
    GTGATGAGCC CCGTCACCAC GCCCACTGAT GAGGACGTGG GCCACAGGAT CAAACATGTG
    GCAGGTTCCA CACAGACGCG GCACATCCCC GAGGACAGCC CCAGCGGTTT CCACCTGCAG
    AGCGTGTCCA AGCTGCTGCT GGTTATCAGC TGTGTTCTGG TGCTGCTGGT CATCCTCAAC
    GTGATGCTCT TCTACAAACT CTGGATGCTG GAGTACACCA CCCAGACCCT CACTGCCTGG
    CAGGGTCTCC GGCTCCAAGA AAGGTTACCC CAGTCCCAGA CAGAGTGGGC CCAGCTCTTA
    GAGTCCCAGC AAAAGTACCA CGATACTGAG CTCCAGAAAT GGAGGGAGAT CATCAAATCC
    TCGGTGATGC TTCTTGACCA GATGAAGGAC TCACTCATCA ATCTTCAGAA CGGCATCAGG
    TCCCGAGACT ACACGTCAGA AAGCGAAGAG AAGAGGAACC GGTACCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222794
    Clone ID Related Accession (Same CDS sequence) XM_005215956.3
    Accession Version XM_005215956.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCTCTG CACTGTGCTC CACTACCTTC CCAATGGCTG CCAGCACTGC CAGTAACTCC 
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGAAAAG CCAGAGCTGG TATAATGTGT TAAGCCCCAC ATACAAGCAG
    AGAAATGAAG ACTTCAGAAA GCTCTTTAAG CAACTTCCAG ACACGGAGCG CCTCATTGTT
    GATTACTCCT GTGCACTCCA AAGAGACATT CTTCTTCAGG GCCGACTCTA CCTCTCCGAA
    AACTGGATCT GCTTCTACAG CAACATCTTC CGCTGGGAAA CTCTGCTAAC TGTGCGTTTG
    AAAGACATCT GCTCCATGAC TAAAGAAAAG ACAGCTCGCC TCATCCCCAA TGCCATCCAA
    GTTTGCACTG ACTCAGAAAA GCACTTTTTC ACTTCGTTCG GGGCCCGGGA TCGGACGTAC
    ATGATGATGT TCCGGCTCTG GCAGAATGCT CTCCTGGAAA AGCCCCTGTG CCCCAAGGAG
    CTGTGGCACT TCGTCCACCA GTGCTATGGG AACGAGCTGG GCCTGACCAG TGACGACGAG
    GACTACGTGC CCCCCGATGA CGACTTCAAC ACGATGGGCT ACTGTGAGGA GATCCCCGTA
    GAAGAGAATG AAGTGAACGA CAGCTCATCC AAAAGCAGCA TAGAGACCAA GCCAGATGCC
    AGTCCACAGC TGCCCAAGAA ATCCATCACC AACAGCACAC TGACGTCCAC CGGGAGCAGT
    GAAGCCCCCG TCTCGTTTGA CGGCCTGCCC CTGGAGGAGG AGGTGCTAGA GGGCGACGGG
    TCTCTGGAGA AGGAGCTCGC CATCGACAAC ATCATCGGGG AAAAGATTGA GATCATCGCA
    CCCGTGAACT CCCCTTCGCT GGACTTCAAC GACAACGAGG ACATCCCCAC TGAGCTCAGT
    GACTCTTCAG ACACCCACGA CGAAGGGGAG GTCCAAGCCT TCTACGAGGA CTTGAGTGGC
    CGGCAGTACG TGAACGAAGT CTTCAACTTC AGCGTGGACA AGCTCTATGA CCTCCTGTTC
    ACTGACTCAC CCTTCCAGCG GGACTTCATG GAGCAGCGGC GCTTCTCAGA TATCATCTTC
    CATCCGTGGA AGAAGGAAGA GAATGGAAAC CAGAGCCGAG TGATCCTTTA CACCATCACC
    CTTACCAACC CTCTAGCTCC CAAAACTGCC ACCGTCAGGG AGACGCAGAC CATGTACAAG
    GCGAGCCAGG AGAGCGAGTG CTATGTGATC GACGCCGAGG TCCTCACGCA CGATGTACCG
    TATCACGACT ACTTCTACAC CATCAACCGC TACACGCTCA CCCGCGTGGC CCGCAACAAG
    AGTCGACTCA GGGTCTCCAC GGAGCTGCGC TATCGGAAAC AGCCCTGGGG GCTGGTGAAA
    ACATTCATCG AGAAGAACTT CTGGAGTGGG CTGGAGGACT ACTTCCGCCA CCTGGAGAGT
    GAGCTGACCA AAACAGAGAG CACCTACCTG GCGGAGATGC ACAGACAGTC TCCTAAAGAG
    AAGGCCAGCA AGCCCCCAAC GGTGCGGAGG AGAAAACGGC CCCATGCCCA CCTGCGGGTG
    CCACACCTGG AGGAGGTGAT GAGCCCCGTC ACCACGCCCA CTGATGAGGA CGTGGGCCAC
    AGGATCAAAC ATGTGGCAGG TTCCACACAG ACGCGGCACA TCCCCGAGGA CAGCCCCAGC
    GGTTTCCACC TGCAGAGCGT GTCCAAGCTG CTGCTGGTTA TCAGCTGTGT TCTGGTGCTG
    CTGGTCATCC TCAACGTGAT GCTCTTCTAC AAACTCTGGA TGCTGGAGTA CACCACCCAG
    ACCCTCACTG CCTGGCAGGG TCTCCGGCTC CAAGAAAGGT TACCCCAGTC CCAGACAGAG
    TGGGCCCAGC TCTTAGAGTC CCAGCAAAAG TACCACGATA CTGAGCTCCA GAAATGGAGG
    GAGATCATCA AATCCTCGGT GATGCTTCTT GACCAGATGA AGGACTCACT CATCAATCTT
    CAGAACGGCA TCAGGTCCCG AGACTACACG TCAGAAAGCG AAGAGAAGAG GAACCGGTAC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216013.2
    CDS244..2469
    Translation

    Target ORF information:

    RefSeq Version XM_005215956.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215956.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGCTCTG CACTGTGCTC CACTACCTTC CCAATGGCTG CCAGCACTGC CAGTAACTCC 
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGAAAAG CCAGAGCTGG TATAATGTGT TAAGCCCCAC ATACAAGCAG
    AGAAATGAAG ACTTCAGAAA GCTCTTTAAG CAACTTCCAG ACACGGAGCG CCTCATTGTT
    GATTACTCCT GTGCACTCCA AAGAGACATT CTTCTTCAGG GCCGACTCTA CCTCTCCGAA
    AACTGGATCT GCTTCTACAG CAACATCTTC CGCTGGGAAA CTCTGCTAAC TGTGCGTTTG
    AAAGACATCT GCTCCATGAC TAAAGAAAAG ACAGCTCGCC TCATCCCCAA TGCCATCCAA
    GTTTGCACTG ACTCAGAAAA GCACTTTTTC ACTTCGTTCG GGGCCCGGGA TCGGACGTAC
    ATGATGATGT TCCGGCTCTG GCAGAATGCT CTCCTGGAAA AGCCCCTGTG CCCCAAGGAG
    CTGTGGCACT TCGTCCACCA GTGCTATGGG AACGAGCTGG GCCTGACCAG TGACGACGAG
    GACTACGTGC CCCCCGATGA CGACTTCAAC ACGATGGGCT ACTGTGAGGA GATCCCCGTA
    GAAGAGAATG AAGTGAACGA CAGCTCATCC AAAAGCAGCA TAGAGACCAA GCCAGATGCC
    AGTCCACAGC TGCCCAAGAA ATCCATCACC AACAGCACAC TGACGTCCAC CGGGAGCAGT
    GAAGCCCCCG TCTCGTTTGA CGGCCTGCCC CTGGAGGAGG AGGTGCTAGA GGGCGACGGG
    TCTCTGGAGA AGGAGCTCGC CATCGACAAC ATCATCGGGG AAAAGATTGA GATCATCGCA
    CCCGTGAACT CCCCTTCGCT GGACTTCAAC GACAACGAGG ACATCCCCAC TGAGCTCAGT
    GACTCTTCAG ACACCCACGA CGAAGGGGAG GTCCAAGCCT TCTACGAGGA CTTGAGTGGC
    CGGCAGTACG TGAACGAAGT CTTCAACTTC AGCGTGGACA AGCTCTATGA CCTCCTGTTC
    ACTGACTCAC CCTTCCAGCG GGACTTCATG GAGCAGCGGC GCTTCTCAGA TATCATCTTC
    CATCCGTGGA AGAAGGAAGA GAATGGAAAC CAGAGCCGAG TGATCCTTTA CACCATCACC
    CTTACCAACC CTCTAGCTCC CAAAACTGCC ACCGTCAGGG AGACGCAGAC CATGTACAAG
    GCGAGCCAGG AGAGCGAGTG CTATGTGATC GACGCCGAGG TCCTCACGCA CGATGTACCG
    TATCACGACT ACTTCTACAC CATCAACCGC TACACGCTCA CCCGCGTGGC CCGCAACAAG
    AGTCGACTCA GGGTCTCCAC GGAGCTGCGC TATCGGAAAC AGCCCTGGGG GCTGGTGAAA
    ACATTCATCG AGAAGAACTT CTGGAGTGGG CTGGAGGACT ACTTCCGCCA CCTGGAGAGT
    GAGCTGACCA AAACAGAGAG CACCTACCTG GCGGAGATGC ACAGACAGTC TCCTAAAGAG
    AAGGCCAGCA AGCCCCCAAC GGTGCGGAGG AGAAAACGGC CCCATGCCCA CCTGCGGGTG
    CCACACCTGG AGGAGGTGAT GAGCCCCGTC ACCACGCCCA CTGATGAGGA CGTGGGCCAC
    AGGATCAAAC ATGTGGCAGG TTCCACACAG ACGCGGCACA TCCCCGAGGA CAGCCCCAGC
    GGTTTCCACC TGCAGAGCGT GTCCAAGCTG CTGCTGGTTA TCAGCTGTGT TCTGGTGCTG
    CTGGTCATCC TCAACGTGAT GCTCTTCTAC AAACTCTGGA TGCTGGAGTA CACCACCCAG
    ACCCTCACTG CCTGGCAGGG TCTCCGGCTC CAAGAAAGGT TACCCCAGTC CCAGACAGAG
    TGGGCCCAGC TCTTAGAGTC CCAGCAAAAG TACCACGATA CTGAGCTCCA GAAATGGAGG
    GAGATCATCA AATCCTCGGT GATGCTTCTT GACCAGATGA AGGACTCACT CATCAATCTT
    CAGAACGGCA TCAGGTCCCG AGACTACACG TCAGAAAGCG AAGAGAAGAG GAACCGGTAC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222795
    Clone ID Related Accession (Same CDS sequence) XM_024975270.1
    Accession Version XM_024975270.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAAGGAT TCAAGCTGTC CTGCACTGCC AGTAACTCCA ACCGCAGCAC GCCAGCCTGC 
    TCCCCCATCC TCCGGAAGCG GTCTCGCTCA CCAACCCCGC AGAACGTAGA CGGAGACACC
    ATGGTGGAGA AGGGCTCAGA TCACTCCTCG GACAAGTCGC CGTCCACTCC GGAGCAGGGC
    GTGCAGCGCA GCTGCTCCTC ACAGTCCGGC CGCAGTGGTG GCAAAAACTC CAAGAAAAGC
    CAGAGCTGGT ATAATGTGTT AAGCCCCACA TACAAGCAGA GAAATGAAGA CTTCAGAAAG
    CTCTTTAAGC AACTTCCAGA CACGGAGCGC CTCATTGTTG ATTACTCCTG TGCACTCCAA
    AGAGACATTC TTCTTCAGGG CCGACTCTAC CTCTCCGAAA ACTGGATCTG CTTCTACAGC
    AACATCTTCC GCTGGGAAAC TCTGCTAACT GTGCGTTTGA AAGACATCTG CTCCATGACT
    AAAGAAAAGA CAGCTCGCCT CATCCCCAAT GCCATCCAAG TTTGCACTGA CTCAGAAAAG
    CACTTTTTCA CTTCGTTCGG GGCCCGGGAT CGGACGTACA TGATGATGTT CCGGCTCTGG
    CAGAATGCTC TCCTGGAAAA GCCCCTGTGC CCCAAGGAGC TGTGGCACTT CGTCCACCAG
    TGCTATGGGA ACGAGCTGGG CCTGACCAGT GACGACGAGG ACTACGTGCC CCCCGATGAC
    GACTTCAACA CGATGGGCTA CTGTGAGGAG ATCCCCGTAG AAGAGAATGA AGTGAACGAC
    AGCTCATCCA AAAGCAGCAT AGAGACCAAG CCAGATGCCA GTCCACAGCT GCCCAAGAAA
    TCCATCACCA ACAGCACACT GACGTCCACC GGGAGCAGTG AAGCCCCCGT CTCGTTTGAC
    GGCCTGCCCC TGGAGGAGGA GGTGCTAGAG GGCGACGGGT CTCTGGAGAA GGAGCTCGCC
    ATCGACAACA TCATCGGGGA AAAGATTGAG ATCATCGCAC CCGTGAACTC CCCTTCGCTG
    GACTTCAACG ACAACGAGGA CATCCCCACT GAGCTCAGTG ACTCTTCAGA CACCCACGAC
    GAAGGGGAGG TCCAAGCCTT CTACGAGGAC TTGAGTGGCC GGCAGTACGT GAACGAAGTC
    TTCAACTTCA GCGTGGACAA GCTCTATGAC CTCCTGTTCA CTGACTCACC CTTCCAGCGG
    GACTTCATGG AGCAGCGGCG CTTCTCAGAT ATCATCTTCC ATCCGTGGAA GAAGGAAGAG
    AATGGAAACC AGAGCCGAGT GATCCTTTAC ACCATCACCC TTACCAACCC TCTAGCTCCC
    AAAACTGCCA CCGTCAGGGA GACGCAGACC ATGTACAAGG CGAGCCAGGA GAGCGAGTGC
    TATGTGATCG ACGCCGAGGT CCTCACGCAC GATGTACCGT ATCACGACTA CTTCTACACC
    ATCAACCGCT ACACGCTCAC CCGCGTGGCC CGCAACAAGA GTCGACTCAG GGTCTCCACG
    GAGCTGCGCT ATCGGAAACA GCCCTGGGGG CTGGTGAAAA CATTCATCGA GAAGAACTTC
    TGGAGTGGGC TGGAGGACTA CTTCCGCCAC CTGGAGAGTG AGCTGACCAA AACAGAGAGC
    ACCTACCTGG CGGAGATGCA CAGACAGTCT CCTAAAGAGA AGGCCAGCAA GCCCCCAACG
    GTGCGGAGGA GAAAACGGCC CCATGCCCAC CTGCGGGTGC CACACCTGGA GGAGGTGATG
    AGCCCCGTCA CCACGCCCAC TGATGAGGAC GTGGGCCACA GGATCAAACA TGTGGCAGGT
    TCCACACAGA CGCGGCACAT CCCCGAGGAC AGCCCCAGCG GTTTCCACCT GCAGAGCGTG
    TCCAAGCTGC TGCTGGTTAT CAGCTGTGTT CTGGTGCTGC TGGTCATCCT CAACGTGATG
    CTCTTCTACA AACTCTGGAT GCTGGAGTAC ACCACCCAGA CCCTCACTGC CTGGCAGGGT
    CTCCGGCTCC AAGAAAGGTT ACCCCAGTCC CAGACAGAGT GGGCCCAGCT CTTAGAGTCC
    CAGCAAAAGT ACCACGATAC TGAGCTCCAG AAATGGAGGG AGATCATCAA ATCCTCGGTG
    ATGCTTCTTG ACCAGATGAA GGACTCACTC ATCAATCTTC AGAACGGCAT CAGGTCCCGA
    GACTACACGT CAGAAAGCGA AGAGAAGAGG AACCGGTACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831038.1
    CDS463..2667
    Translation

    Target ORF information:

    RefSeq Version XM_024975270.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975270.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAAGGAT TCAAGCTGTC CTGCACTGCC AGTAACTCCA ACCGCAGCAC GCCAGCCTGC 
    TCCCCCATCC TCCGGAAGCG GTCTCGCTCA CCAACCCCGC AGAACGTAGA CGGAGACACC
    ATGGTGGAGA AGGGCTCAGA TCACTCCTCG GACAAGTCGC CGTCCACTCC GGAGCAGGGC
    GTGCAGCGCA GCTGCTCCTC ACAGTCCGGC CGCAGTGGTG GCAAAAACTC CAAGAAAAGC
    CAGAGCTGGT ATAATGTGTT AAGCCCCACA TACAAGCAGA GAAATGAAGA CTTCAGAAAG
    CTCTTTAAGC AACTTCCAGA CACGGAGCGC CTCATTGTTG ATTACTCCTG TGCACTCCAA
    AGAGACATTC TTCTTCAGGG CCGACTCTAC CTCTCCGAAA ACTGGATCTG CTTCTACAGC
    AACATCTTCC GCTGGGAAAC TCTGCTAACT GTGCGTTTGA AAGACATCTG CTCCATGACT
    AAAGAAAAGA CAGCTCGCCT CATCCCCAAT GCCATCCAAG TTTGCACTGA CTCAGAAAAG
    CACTTTTTCA CTTCGTTCGG GGCCCGGGAT CGGACGTACA TGATGATGTT CCGGCTCTGG
    CAGAATGCTC TCCTGGAAAA GCCCCTGTGC CCCAAGGAGC TGTGGCACTT CGTCCACCAG
    TGCTATGGGA ACGAGCTGGG CCTGACCAGT GACGACGAGG ACTACGTGCC CCCCGATGAC
    GACTTCAACA CGATGGGCTA CTGTGAGGAG ATCCCCGTAG AAGAGAATGA AGTGAACGAC
    AGCTCATCCA AAAGCAGCAT AGAGACCAAG CCAGATGCCA GTCCACAGCT GCCCAAGAAA
    TCCATCACCA ACAGCACACT GACGTCCACC GGGAGCAGTG AAGCCCCCGT CTCGTTTGAC
    GGCCTGCCCC TGGAGGAGGA GGTGCTAGAG GGCGACGGGT CTCTGGAGAA GGAGCTCGCC
    ATCGACAACA TCATCGGGGA AAAGATTGAG ATCATCGCAC CCGTGAACTC CCCTTCGCTG
    GACTTCAACG ACAACGAGGA CATCCCCACT GAGCTCAGTG ACTCTTCAGA CACCCACGAC
    GAAGGGGAGG TCCAAGCCTT CTACGAGGAC TTGAGTGGCC GGCAGTACGT GAACGAAGTC
    TTCAACTTCA GCGTGGACAA GCTCTATGAC CTCCTGTTCA CTGACTCACC CTTCCAGCGG
    GACTTCATGG AGCAGCGGCG CTTCTCAGAT ATCATCTTCC ATCCGTGGAA GAAGGAAGAG
    AATGGAAACC AGAGCCGAGT GATCCTTTAC ACCATCACCC TTACCAACCC TCTAGCTCCC
    AAAACTGCCA CCGTCAGGGA GACGCAGACC ATGTACAAGG CGAGCCAGGA GAGCGAGTGC
    TATGTGATCG ACGCCGAGGT CCTCACGCAC GATGTACCGT ATCACGACTA CTTCTACACC
    ATCAACCGCT ACACGCTCAC CCGCGTGGCC CGCAACAAGA GTCGACTCAG GGTCTCCACG
    GAGCTGCGCT ATCGGAAACA GCCCTGGGGG CTGGTGAAAA CATTCATCGA GAAGAACTTC
    TGGAGTGGGC TGGAGGACTA CTTCCGCCAC CTGGAGAGTG AGCTGACCAA AACAGAGAGC
    ACCTACCTGG CGGAGATGCA CAGACAGTCT CCTAAAGAGA AGGCCAGCAA GCCCCCAACG
    GTGCGGAGGA GAAAACGGCC CCATGCCCAC CTGCGGGTGC CACACCTGGA GGAGGTGATG
    AGCCCCGTCA CCACGCCCAC TGATGAGGAC GTGGGCCACA GGATCAAACA TGTGGCAGGT
    TCCACACAGA CGCGGCACAT CCCCGAGGAC AGCCCCAGCG GTTTCCACCT GCAGAGCGTG
    TCCAAGCTGC TGCTGGTTAT CAGCTGTGTT CTGGTGCTGC TGGTCATCCT CAACGTGATG
    CTCTTCTACA AACTCTGGAT GCTGGAGTAC ACCACCCAGA CCCTCACTGC CTGGCAGGGT
    CTCCGGCTCC AAGAAAGGTT ACCCCAGTCC CAGACAGAGT GGGCCCAGCT CTTAGAGTCC
    CAGCAAAAGT ACCACGATAC TGAGCTCCAG AAATGGAGGG AGATCATCAA ATCCTCGGTG
    ATGCTTCTTG ACCAGATGAA GGACTCACTC ATCAATCTTC AGAACGGCAT CAGGTCCCGA
    GACTACACGT CAGAAAGCGA AGAGAAGAGG AACCGGTACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92652
    Clone ID Related Accession (Same CDS sequence) XM_015474651.1 , XM_015474650.1
    Accession Version XM_015474651.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTGGAGA AGGGCTCAGA TCACTCCTCG GACAAGTCGC CGTCCACTCC GGAGCAGGGC 
    GTGCAGCGCA GCTGCTCCTC ACAGTCCGGC CGCAGTGGTG GCAAAAACTC CAAGTCTCAC
    AAGCGCCTCT CAAAATACGA CAGACTTTAC CTGATCAAAA AAAGCCAGAG CTGGTATAAT
    GTGTTAAGCC CCACATACAA GCAGAGAAAT GAAGACTTCA GAAAGCTCTT TAAGCAACTT
    CCAGACACGG AGCGCCTCAT TGTTGATTAC TCCTGTGCAC TCCAAAGAGA CATTCTTCTT
    CAGGGCCGAC TCTACCTCTC CGAAAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG
    GAAACTCTGC TAACTGTGCG TTTGAAAGAC ATCTGCTCCA TGACTAAAGA AAAGACAGCT
    CGCCTCATCC CCAATGCCAT CCAAGTTTGC ACTGACTCAG AAAAGCACTT TTTCACTTCG
    TTCGGGGCCC GGGATCGGAC GTACATGATG ATGTTCCGGC TCTGGCAGAA TGCTCTCCTG
    GAAAAGCCCC TGTGCCCCAA GGAGCTGTGG CACTTCGTCC ACCAGTGCTA TGGGAACGAG
    CTGGGCCTGA CCAGTGACGA CGAGGACTAC GTGCCCCCCG ATGACGACTT CAACACGATG
    GGCTACTGTG AGGAGATCCC CGTAGAAGAG AATGAAGTGA ACGACAGCTC ATCCAAAAGC
    AGCATAGAGA CCAAGCCAGA TGCCAGTCCA CAGCTGCCCA AGAAATCCAT CACCAACAGC
    ACACTGACGT CCACCGGGAG CAGTGAAGCC CCCGTCTCGT TTGACGGCCT GCCCCTGGAG
    GAGGAGGTGC TAGAGGGCGA CGGGTCTCTG GAGAAGGAGC TCGCCATCGA CAACATCATC
    GGGGAAAAGA TTGAGATCAT CGCGCCCGTG AACTCCCCTT CGCTGGACTT CAACGACAAC
    GAGGACATCC CCACTGAGCT CAGTGACTCT TCAGACACCC ACGACGAAGG GGAGGTCCAA
    GCCTTCTACG AGGACTTGAG TGGCCGGCAG TACGTGAACG AAGTCTTCAA CTTCAGCGTG
    GACAAGCTCT ATGACCTCCT GTTCACTGAC TCACCCTTCC AGCGGGACTT CATGGAGCAG
    CGGCGCTTCT CAGATATCAT CTTCCATCCG TGGAAGAAGG AAGAGAATGG AAACCAGAGC
    CGAGTGATCC TTTACACCAT CACCCTTACC AACCCTCTAG CTCCCAAAAC TGCCACCGTC
    AGGGAGACAC AGACCATGTA CAAGGCGAGC CAGGAGAGCG AGTGCTATGT GATCGACGCC
    GAGGTCCTCA CGCACGATGT ACCGTATCAC GACTACTTCT ACACCATCAA CCGCTACACG
    CTTACCCGCG TGGCCCGCAA CAAGAGTCGA CTCAGGGTCT CCACGGAGCT GCGCTATCGG
    AAACAGCCCT GGGGGCTGGT GAAAACATTC ATCGAGAAGA ACTTCTGGAG TGGGCTGGAG
    GACTACTTCC GCCACCTGGA GAGTGAGCTG ACCAAAACAG AGAGCACCTA CCTGGCGGAG
    ATGCACAGAC AGTCTCCTAA AGAGAAGGCC AGCAAGCCCC CAACGGTGCG GAGGAGAAAA
    CGGCCCCATG CCCACCTGCG GGTGCCACAC CTGGAGGAGG TGATGAGCCC CGTCACCACG
    CCCACTGATG AGGACGTGGG CCACAGGATC AAACATGTGG CAGGTTCCAC ACAGACACGG
    CACATCCCCG AGGACGGCCC CAGCGGTTTC CACCTGCAGA GCGTGTCCAA GCTGCTGCTG
    GTTATCAGCT GTGTTCTGGT GCTGCTGGTC ATCCTCAACG TGATGCTCTT CTACAAACTC
    TGGATGCTGG AGTACACCAC CCAGACCCTC ACTGCCTGGC AGGGTCTCCG GCTCCAAGAA
    AGGTTACCCC AGTCCCAGAC AGAGTGGGCC CAGCTCTTAG AGTCCCAGCA AAAGTACCAC
    GATACTGAGC TCCAGAAATG GAGGGAGATC ATCAAATCCT CGGTGATGCT TCTTGACCAG
    ATGAAGGACT CACTCATCAA TCTTCAGAAC GGCATCAGGT CCCGAGACTA CACGTCAGAA
    AGCGAAGAGA AGAGGAACCG GTACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330137.1
    CDS119..2248
    Translation

    Target ORF information:

    RefSeq Version XM_015474651.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTGGAGA AGGGCTCAGA TCACTCCTCG GACAAGTCGC CGTCCACTCC GGAGCAGGGC 
    GTGCAGCGCA GCTGCTCCTC ACAGTCCGGC CGCAGTGGTG GCAAAAACTC CAAGTCTCAC
    AAGCGCCTCT CAAAATACGA CAGACTTTAC CTGATCAAAA AAAGCCAGAG CTGGTATAAT
    GTGTTAAGCC CCACATACAA GCAGAGAAAT GAAGACTTCA GAAAGCTCTT TAAGCAACTT
    CCAGACACGG AGCGCCTCAT TGTTGATTAC TCCTGTGCAC TCCAAAGAGA CATTCTTCTT
    CAGGGCCGAC TCTACCTCTC CGAAAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG
    GAAACTCTGC TAACTGTGCG TTTGAAAGAC ATCTGCTCCA TGACTAAAGA AAAGACAGCT
    CGCCTCATCC CCAATGCCAT CCAAGTTTGC ACTGACTCAG AAAAGCACTT TTTCACTTCG
    TTCGGGGCCC GGGATCGGAC GTACATGATG ATGTTCCGGC TCTGGCAGAA TGCTCTCCTG
    GAAAAGCCCC TGTGCCCCAA GGAGCTGTGG CACTTCGTCC ACCAGTGCTA TGGGAACGAG
    CTGGGCCTGA CCAGTGACGA CGAGGACTAC GTGCCCCCCG ATGACGACTT CAACACGATG
    GGCTACTGTG AGGAGATCCC CGTAGAAGAG AATGAAGTGA ACGACAGCTC ATCCAAAAGC
    AGCATAGAGA CCAAGCCAGA TGCCAGTCCA CAGCTGCCCA AGAAATCCAT CACCAACAGC
    ACACTGACGT CCACCGGGAG CAGTGAAGCC CCCGTCTCGT TTGACGGCCT GCCCCTGGAG
    GAGGAGGTGC TAGAGGGCGA CGGGTCTCTG GAGAAGGAGC TCGCCATCGA CAACATCATC
    GGGGAAAAGA TTGAGATCAT CGCGCCCGTG AACTCCCCTT CGCTGGACTT CAACGACAAC
    GAGGACATCC CCACTGAGCT CAGTGACTCT TCAGACACCC ACGACGAAGG GGAGGTCCAA
    GCCTTCTACG AGGACTTGAG TGGCCGGCAG TACGTGAACG AAGTCTTCAA CTTCAGCGTG
    GACAAGCTCT ATGACCTCCT GTTCACTGAC TCACCCTTCC AGCGGGACTT CATGGAGCAG
    CGGCGCTTCT CAGATATCAT CTTCCATCCG TGGAAGAAGG AAGAGAATGG AAACCAGAGC
    CGAGTGATCC TTTACACCAT CACCCTTACC AACCCTCTAG CTCCCAAAAC TGCCACCGTC
    AGGGAGACAC AGACCATGTA CAAGGCGAGC CAGGAGAGCG AGTGCTATGT GATCGACGCC
    GAGGTCCTCA CGCACGATGT ACCGTATCAC GACTACTTCT ACACCATCAA CCGCTACACG
    CTTACCCGCG TGGCCCGCAA CAAGAGTCGA CTCAGGGTCT CCACGGAGCT GCGCTATCGG
    AAACAGCCCT GGGGGCTGGT GAAAACATTC ATCGAGAAGA ACTTCTGGAG TGGGCTGGAG
    GACTACTTCC GCCACCTGGA GAGTGAGCTG ACCAAAACAG AGAGCACCTA CCTGGCGGAG
    ATGCACAGAC AGTCTCCTAA AGAGAAGGCC AGCAAGCCCC CAACGGTGCG GAGGAGAAAA
    CGGCCCCATG CCCACCTGCG GGTGCCACAC CTGGAGGAGG TGATGAGCCC CGTCACCACG
    CCCACTGATG AGGACGTGGG CCACAGGATC AAACATGTGG CAGGTTCCAC ACAGACACGG
    CACATCCCCG AGGACGGCCC CAGCGGTTTC CACCTGCAGA GCGTGTCCAA GCTGCTGCTG
    GTTATCAGCT GTGTTCTGGT GCTGCTGGTC ATCCTCAACG TGATGCTCTT CTACAAACTC
    TGGATGCTGG AGTACACCAC CCAGACCCTC ACTGCCTGGC AGGGTCTCCG GCTCCAAGAA
    AGGTTACCCC AGTCCCAGAC AGAGTGGGCC CAGCTCTTAG AGTCCCAGCA AAAGTACCAC
    GATACTGAGC TCCAGAAATG GAGGGAGATC ATCAAATCCT CGGTGATGCT TCTTGACCAG
    ATGAAGGACT CACTCATCAA TCTTCAGAAC GGCATCAGGT CCCGAGACTA CACGTCAGAA
    AGCGAAGAGA AGAGGAACCG GTACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92652
    Clone ID Related Accession (Same CDS sequence) XM_015474651.1 , XM_015474650.1
    Accession Version XM_015474650.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTGGAGA AGGGCTCAGA TCACTCCTCG GACAAGTCGC CGTCCACTCC GGAGCAGGGC 
    GTGCAGCGCA GCTGCTCCTC ACAGTCCGGC CGCAGTGGTG GCAAAAACTC CAAGTCTCAC
    AAGCGCCTCT CAAAATACGA CAGACTTTAC CTGATCAAAA AAAGCCAGAG CTGGTATAAT
    GTGTTAAGCC CCACATACAA GCAGAGAAAT GAAGACTTCA GAAAGCTCTT TAAGCAACTT
    CCAGACACGG AGCGCCTCAT TGTTGATTAC TCCTGTGCAC TCCAAAGAGA CATTCTTCTT
    CAGGGCCGAC TCTACCTCTC CGAAAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG
    GAAACTCTGC TAACTGTGCG TTTGAAAGAC ATCTGCTCCA TGACTAAAGA AAAGACAGCT
    CGCCTCATCC CCAATGCCAT CCAAGTTTGC ACTGACTCAG AAAAGCACTT TTTCACTTCG
    TTCGGGGCCC GGGATCGGAC GTACATGATG ATGTTCCGGC TCTGGCAGAA TGCTCTCCTG
    GAAAAGCCCC TGTGCCCCAA GGAGCTGTGG CACTTCGTCC ACCAGTGCTA TGGGAACGAG
    CTGGGCCTGA CCAGTGACGA CGAGGACTAC GTGCCCCCCG ATGACGACTT CAACACGATG
    GGCTACTGTG AGGAGATCCC CGTAGAAGAG AATGAAGTGA ACGACAGCTC ATCCAAAAGC
    AGCATAGAGA CCAAGCCAGA TGCCAGTCCA CAGCTGCCCA AGAAATCCAT CACCAACAGC
    ACACTGACGT CCACCGGGAG CAGTGAAGCC CCCGTCTCGT TTGACGGCCT GCCCCTGGAG
    GAGGAGGTGC TAGAGGGCGA CGGGTCTCTG GAGAAGGAGC TCGCCATCGA CAACATCATC
    GGGGAAAAGA TTGAGATCAT CGCGCCCGTG AACTCCCCTT CGCTGGACTT CAACGACAAC
    GAGGACATCC CCACTGAGCT CAGTGACTCT TCAGACACCC ACGACGAAGG GGAGGTCCAA
    GCCTTCTACG AGGACTTGAG TGGCCGGCAG TACGTGAACG AAGTCTTCAA CTTCAGCGTG
    GACAAGCTCT ATGACCTCCT GTTCACTGAC TCACCCTTCC AGCGGGACTT CATGGAGCAG
    CGGCGCTTCT CAGATATCAT CTTCCATCCG TGGAAGAAGG AAGAGAATGG AAACCAGAGC
    CGAGTGATCC TTTACACCAT CACCCTTACC AACCCTCTAG CTCCCAAAAC TGCCACCGTC
    AGGGAGACAC AGACCATGTA CAAGGCGAGC CAGGAGAGCG AGTGCTATGT GATCGACGCC
    GAGGTCCTCA CGCACGATGT ACCGTATCAC GACTACTTCT ACACCATCAA CCGCTACACG
    CTTACCCGCG TGGCCCGCAA CAAGAGTCGA CTCAGGGTCT CCACGGAGCT GCGCTATCGG
    AAACAGCCCT GGGGGCTGGT GAAAACATTC ATCGAGAAGA ACTTCTGGAG TGGGCTGGAG
    GACTACTTCC GCCACCTGGA GAGTGAGCTG ACCAAAACAG AGAGCACCTA CCTGGCGGAG
    ATGCACAGAC AGTCTCCTAA AGAGAAGGCC AGCAAGCCCC CAACGGTGCG GAGGAGAAAA
    CGGCCCCATG CCCACCTGCG GGTGCCACAC CTGGAGGAGG TGATGAGCCC CGTCACCACG
    CCCACTGATG AGGACGTGGG CCACAGGATC AAACATGTGG CAGGTTCCAC ACAGACACGG
    CACATCCCCG AGGACGGCCC CAGCGGTTTC CACCTGCAGA GCGTGTCCAA GCTGCTGCTG
    GTTATCAGCT GTGTTCTGGT GCTGCTGGTC ATCCTCAACG TGATGCTCTT CTACAAACTC
    TGGATGCTGG AGTACACCAC CCAGACCCTC ACTGCCTGGC AGGGTCTCCG GCTCCAAGAA
    AGGTTACCCC AGTCCCAGAC AGAGTGGGCC CAGCTCTTAG AGTCCCAGCA AAAGTACCAC
    GATACTGAGC TCCAGAAATG GAGGGAGATC ATCAAATCCT CGGTGATGCT TCTTGACCAG
    ATGAAGGACT CACTCATCAA TCTTCAGAAC GGCATCAGGT CCCGAGACTA CACGTCAGAA
    AGCGAAGAGA AGAGGAACCG GTACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330136.1
    CDS122..2251
    Translation

    Target ORF information:

    RefSeq Version XM_015474650.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474650.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTGGAGA AGGGCTCAGA TCACTCCTCG GACAAGTCGC CGTCCACTCC GGAGCAGGGC 
    GTGCAGCGCA GCTGCTCCTC ACAGTCCGGC CGCAGTGGTG GCAAAAACTC CAAGTCTCAC
    AAGCGCCTCT CAAAATACGA CAGACTTTAC CTGATCAAAA AAAGCCAGAG CTGGTATAAT
    GTGTTAAGCC CCACATACAA GCAGAGAAAT GAAGACTTCA GAAAGCTCTT TAAGCAACTT
    CCAGACACGG AGCGCCTCAT TGTTGATTAC TCCTGTGCAC TCCAAAGAGA CATTCTTCTT
    CAGGGCCGAC TCTACCTCTC CGAAAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG
    GAAACTCTGC TAACTGTGCG TTTGAAAGAC ATCTGCTCCA TGACTAAAGA AAAGACAGCT
    CGCCTCATCC CCAATGCCAT CCAAGTTTGC ACTGACTCAG AAAAGCACTT TTTCACTTCG
    TTCGGGGCCC GGGATCGGAC GTACATGATG ATGTTCCGGC TCTGGCAGAA TGCTCTCCTG
    GAAAAGCCCC TGTGCCCCAA GGAGCTGTGG CACTTCGTCC ACCAGTGCTA TGGGAACGAG
    CTGGGCCTGA CCAGTGACGA CGAGGACTAC GTGCCCCCCG ATGACGACTT CAACACGATG
    GGCTACTGTG AGGAGATCCC CGTAGAAGAG AATGAAGTGA ACGACAGCTC ATCCAAAAGC
    AGCATAGAGA CCAAGCCAGA TGCCAGTCCA CAGCTGCCCA AGAAATCCAT CACCAACAGC
    ACACTGACGT CCACCGGGAG CAGTGAAGCC CCCGTCTCGT TTGACGGCCT GCCCCTGGAG
    GAGGAGGTGC TAGAGGGCGA CGGGTCTCTG GAGAAGGAGC TCGCCATCGA CAACATCATC
    GGGGAAAAGA TTGAGATCAT CGCGCCCGTG AACTCCCCTT CGCTGGACTT CAACGACAAC
    GAGGACATCC CCACTGAGCT CAGTGACTCT TCAGACACCC ACGACGAAGG GGAGGTCCAA
    GCCTTCTACG AGGACTTGAG TGGCCGGCAG TACGTGAACG AAGTCTTCAA CTTCAGCGTG
    GACAAGCTCT ATGACCTCCT GTTCACTGAC TCACCCTTCC AGCGGGACTT CATGGAGCAG
    CGGCGCTTCT CAGATATCAT CTTCCATCCG TGGAAGAAGG AAGAGAATGG AAACCAGAGC
    CGAGTGATCC TTTACACCAT CACCCTTACC AACCCTCTAG CTCCCAAAAC TGCCACCGTC
    AGGGAGACAC AGACCATGTA CAAGGCGAGC CAGGAGAGCG AGTGCTATGT GATCGACGCC
    GAGGTCCTCA CGCACGATGT ACCGTATCAC GACTACTTCT ACACCATCAA CCGCTACACG
    CTTACCCGCG TGGCCCGCAA CAAGAGTCGA CTCAGGGTCT CCACGGAGCT GCGCTATCGG
    AAACAGCCCT GGGGGCTGGT GAAAACATTC ATCGAGAAGA ACTTCTGGAG TGGGCTGGAG
    GACTACTTCC GCCACCTGGA GAGTGAGCTG ACCAAAACAG AGAGCACCTA CCTGGCGGAG
    ATGCACAGAC AGTCTCCTAA AGAGAAGGCC AGCAAGCCCC CAACGGTGCG GAGGAGAAAA
    CGGCCCCATG CCCACCTGCG GGTGCCACAC CTGGAGGAGG TGATGAGCCC CGTCACCACG
    CCCACTGATG AGGACGTGGG CCACAGGATC AAACATGTGG CAGGTTCCAC ACAGACACGG
    CACATCCCCG AGGACGGCCC CAGCGGTTTC CACCTGCAGA GCGTGTCCAA GCTGCTGCTG
    GTTATCAGCT GTGTTCTGGT GCTGCTGGTC ATCCTCAACG TGATGCTCTT CTACAAACTC
    TGGATGCTGG AGTACACCAC CCAGACCCTC ACTGCCTGGC AGGGTCTCCG GCTCCAAGAA
    AGGTTACCCC AGTCCCAGAC AGAGTGGGCC CAGCTCTTAG AGTCCCAGCA AAAGTACCAC
    GATACTGAGC TCCAGAAATG GAGGGAGATC ATCAAATCCT CGGTGATGCT TCTTGACCAG
    ATGAAGGACT CACTCATCAA TCTTCAGAAC GGCATCAGGT CCCGAGACTA CACGTCAGAA
    AGCGAAGAGA AGAGGAACCG GTACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02023
    Clone ID Related Accession (Same CDS sequence) NM_001191419.1
    Accession Version NM_001191419.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2012-08-31
    Organism Bos taurus(Cattle)
    Product GRAM domain-containing protein 1B
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03076597.1 and AAFC03088183.1. On Jul 17, 2010 this sequence version replaced XM_002701320.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA GCAGAGCAGC CAGCAGAGCA GCCATGACGA TGACTCCAGC
    AGGTTCCTGA GTCCTCGCGT GCGGGAAGAA AGCACTGCCA GTAACTCCAA CCGCAGCACG
    CCAGCCTGCT CCCCCATCCT CCGGAAGCGG TCTCGCTCAC CAACCCCGCA GAACGTAGAC
    GGAGACACCA TGGTGGAGAA GGGCTCAGAT CACTCCTCGG ACAAGTCGCC GTCCACTCCG
    GAGCAGGGCG TGCAGCGCAG CTGCTCCTCA CAGTCCGGCC GCAGTGGTGG CAAAAACTCC
    AAGAAAAGCC AGAGCTGGTA TAATGTGTTA AGCCCCACAT ACAAGCAGAG AAATGAAGAC
    TTCAGAAAGC TCTTTAAGCA ACTTCCAGAC ACGGAGCGCC TCATTGTTGA TTACTCCTGT
    GCACTCCAAA GAGACATTCT TCTTCAGGGC CGACTCTACC TCTCCGAAAA CTGGATCTGC
    TTCTACAGCA ACATCTTCCG CTGGGAAACT CTGCTAACTG TGCGTTTGAA AGACATCTGC
    TCCATGACTA AAGAAAAGAC AGCTCGCCTC ATCCCCAATG CCATCCAAGT TTGCACTGAC
    TCAGAAAAGC ACTTTTTCAC TTCGTTCGGG GCCCGGGATC GGACGTACAT GATGATGTTC
    CGGCTCTGGC AGAATGCTCT CCTGGAAAAG CCCCTGTGCC CCAAGGAGCT GTGGCACTTC
    GTCCACCAGT GCTATGGGAA CGAGCTGGGC CTGACCAGTG ACGACGAGGA CTACGTGCCC
    CCCGATGACG ACTTCAACAC GATGGGCTAC TGTGAGGAGA TCCCCGTAGA AGAGAATGAA
    GTGAACGACA GCTCATCCAA AAGCAGCATA GAGACCAAGC CGGATGCCAG TCCACAGCTG
    CCCAAGAAAT CCATCACCAA CAGCACACTG ACGTCCACCG GGAGCAGTGA AGCCCCCGTC
    TCGTTTGACG GCCTGCCCCT GGAGGAGGAG GTGCTAGAGG GCGACGGGTC TCTGGAGAAG
    GAGCTCGCCA TCGACAACAT CATCGGGGAA AAGATTGAGA TCATCGCACC CGTGAACTCC
    CCTTCGCTGG ACTTCAACGA CAACGAGGAC ATCCCCACTG AGCTCAGTGA CTCTTCAGAC
    ACCCACGACG AAGGGGAGGT CCAAGCCTTC TACGAGGACT TGAGTGGCCG GCAGTACGTG
    AACGAAGTCT TCAACTTCAG CGTGGACAAG CTCTATGACC TCCTGTTCAC TGACTCACCC
    TTCCAGCGGG ACTTCATGGA GCAGCGGCGC TTCTCAGATA TCATCTTCCA TCCGTGGAAG
    AAGGAAGAGA ATGGAAACCA GAGCCGAGTG ATCCTTTACA CCATCACCCT TACCAACCCT
    CTAGCTCCCA AAACTGCCAC CGTCAGGGAG ACACAGACCA TGTACAAGGC GAGCCAGGAG
    AGCGAGTGCT ATGTGATCGA CGCCGAGGTC CTCACGCACG ATGTACCGTA TCACGACTAC
    TTCTACACCA TCAACCGCTA CACGCTTACC CGCGTGGCCC GCAACAAGAG TCGACTCAGG
    GTCTCCACGG AGCTGCGCTA TCGGAAACAG CCCTGGGGGC TGGTGAAAAC ATTCATCGAG
    AAGAACTTCT GGAGTGGGCT GGAGGACTAC TTCCGCCACC TGGAGAGTGA GCTGACCAAA
    ACAGAGAGCA CCTACCTGGC GGAGATGCAC AGACAGTCTC CTAAAGAGAA GGCCAGCAAG
    CCCCCAACGG TGCGGAGGAG AAAACGGCCC CATGCCCACC TGCGGGTGCC ACACCTGGAG
    GAGGTGATGA GCCCCGTCAC CACGCCCACT GATGAGGACG TGGGCCACAG GATCAAACAT
    GTGGCAGGTT CCACACAGAC ACGGCACATC CCCGAGGACG GCCCCAGCGG TTTCCACCTG
    CAGAGCGTGT CCAAGCTGCT GCTGGTTATC AGCTGTGTTC TGGTGCTGCT GGTCATCCTC
    AACGTGATGC TCTTCTACAA ACTCTGGATG CTGGAGTACA CCACCCAGAC CCTCACTGCC
    TGGCAGGGTC TCCGGCTCCA AGAAAGGTTA CCCCAGTCCC AGACAGAGTG GGCCCAGCTC
    TTAGAGTCCC AGCAAAAGTA CCACGATACT GAGCTCCAGA AATGGAGGGA GATCATCAAA
    TCCTCGGTGA TGCTTCTTGA CCAGATGAAG GACTCACTCA TCAATCTTCA GAACGGCATC
    AGGTCCCGAG ACTACACGTC AGAAAGCGAA GAGAAGAGGA ACCGGTACCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178348.1
    CDS1..2634
    Translation

    Target ORF information:

    RefSeq Version NM_001191419.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191419.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA GCAGAGCAGC CAGCAGAGCA GCCATGACGA TGACTCCAGC
    AGGTTCCTGA GTCCTCGCGT GCGGGAAGAA AGCACTGCCA GTAACTCCAA CCGCAGCACG
    CCAGCCTGCT CCCCCATCCT CCGGAAGCGG TCTCGCTCAC CAACCCCGCA GAACGTAGAC
    GGAGACACCA TGGTGGAGAA GGGCTCAGAT CACTCCTCGG ACAAGTCGCC GTCCACTCCG
    GAGCAGGGCG TGCAGCGCAG CTGCTCCTCA CAGTCCGGCC GCAGTGGTGG CAAAAACTCC
    AAGAAAAGCC AGAGCTGGTA TAATGTGTTA AGCCCCACAT ACAAGCAGAG AAATGAAGAC
    TTCAGAAAGC TCTTTAAGCA ACTTCCAGAC ACGGAGCGCC TCATTGTTGA TTACTCCTGT
    GCACTCCAAA GAGACATTCT TCTTCAGGGC CGACTCTACC TCTCCGAAAA CTGGATCTGC
    TTCTACAGCA ACATCTTCCG CTGGGAAACT CTGCTAACTG TGCGTTTGAA AGACATCTGC
    TCCATGACTA AAGAAAAGAC AGCTCGCCTC ATCCCCAATG CCATCCAAGT TTGCACTGAC
    TCAGAAAAGC ACTTTTTCAC TTCGTTCGGG GCCCGGGATC GGACGTACAT GATGATGTTC
    CGGCTCTGGC AGAATGCTCT CCTGGAAAAG CCCCTGTGCC CCAAGGAGCT GTGGCACTTC
    GTCCACCAGT GCTATGGGAA CGAGCTGGGC CTGACCAGTG ACGACGAGGA CTACGTGCCC
    CCCGATGACG ACTTCAACAC GATGGGCTAC TGTGAGGAGA TCCCCGTAGA AGAGAATGAA
    GTGAACGACA GCTCATCCAA AAGCAGCATA GAGACCAAGC CGGATGCCAG TCCACAGCTG
    CCCAAGAAAT CCATCACCAA CAGCACACTG ACGTCCACCG GGAGCAGTGA AGCCCCCGTC
    TCGTTTGACG GCCTGCCCCT GGAGGAGGAG GTGCTAGAGG GCGACGGGTC TCTGGAGAAG
    GAGCTCGCCA TCGACAACAT CATCGGGGAA AAGATTGAGA TCATCGCACC CGTGAACTCC
    CCTTCGCTGG ACTTCAACGA CAACGAGGAC ATCCCCACTG AGCTCAGTGA CTCTTCAGAC
    ACCCACGACG AAGGGGAGGT CCAAGCCTTC TACGAGGACT TGAGTGGCCG GCAGTACGTG
    AACGAAGTCT TCAACTTCAG CGTGGACAAG CTCTATGACC TCCTGTTCAC TGACTCACCC
    TTCCAGCGGG ACTTCATGGA GCAGCGGCGC TTCTCAGATA TCATCTTCCA TCCGTGGAAG
    AAGGAAGAGA ATGGAAACCA GAGCCGAGTG ATCCTTTACA CCATCACCCT TACCAACCCT
    CTAGCTCCCA AAACTGCCAC CGTCAGGGAG ACACAGACCA TGTACAAGGC GAGCCAGGAG
    AGCGAGTGCT ATGTGATCGA CGCCGAGGTC CTCACGCACG ATGTACCGTA TCACGACTAC
    TTCTACACCA TCAACCGCTA CACGCTTACC CGCGTGGCCC GCAACAAGAG TCGACTCAGG
    GTCTCCACGG AGCTGCGCTA TCGGAAACAG CCCTGGGGGC TGGTGAAAAC ATTCATCGAG
    AAGAACTTCT GGAGTGGGCT GGAGGACTAC TTCCGCCACC TGGAGAGTGA GCTGACCAAA
    ACAGAGAGCA CCTACCTGGC GGAGATGCAC AGACAGTCTC CTAAAGAGAA GGCCAGCAAG
    CCCCCAACGG TGCGGAGGAG AAAACGGCCC CATGCCCACC TGCGGGTGCC ACACCTGGAG
    GAGGTGATGA GCCCCGTCAC CACGCCCACT GATGAGGACG TGGGCCACAG GATCAAACAT
    GTGGCAGGTT CCACACAGAC ACGGCACATC CCCGAGGACG GCCCCAGCGG TTTCCACCTG
    CAGAGCGTGT CCAAGCTGCT GCTGGTTATC AGCTGTGTTC TGGTGCTGCT GGTCATCCTC
    AACGTGATGC TCTTCTACAA ACTCTGGATG CTGGAGTACA CCACCCAGAC CCTCACTGCC
    TGGCAGGGTC TCCGGCTCCA AGAAAGGTTA CCCCAGTCCC AGACAGAGTG GGCCCAGCTC
    TTAGAGTCCC AGCAAAAGTA CCACGATACT GAGCTCCAGA AATGGAGGGA GATCATCAAA
    TCCTCGGTGA TGCTTCTTGA CCAGATGAAG GACTCACTCA TCAATCTTCA GAACGGCATC
    AGGTCCCGAG ACTACACGTC AGAAAGCGAA GAGAAGAGGA ACCGGTACCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216302
    Clone ID Related Accession (Same CDS sequence) NM_001191419.2
    Accession Version NM_001191419.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02040572.1. On Jun 30, 2017 this sequence version replaced NM_001191419.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA GCAGAGCAGC CAGCAGAGCA GCCATGACGA TGACTCCAGC
    AGGTTCCTGA GTCCTCGCGT GCGGGAAGAA AGCACTGCCA GTAACTCCAA CCGCAGCACG
    CCAGCCTGCT CCCCCATCCT CCGGAAGCGG TCTCGCTCAC CAACCCCGCA GAACGTAGAC
    GGAGACACCA TGGTGGAGAA GGGCTCAGAT CACTCCTCGG ACAAGTCGCC GTCCACTCCG
    GAGCAGGGCG TGCAGCGCAG CTGCTCCTCA CAGTCCGGCC GCAGTGGTGG CAAAAACTCC
    AAGAAAAGCC AGAGCTGGTA TAATGTGTTA AGCCCCACAT ACAAGCAGAG AAATGAAGAC
    TTCAGAAAGC TCTTTAAGCA ACTTCCAGAC ACGGAGCGCC TCATTGTTGA TTACTCCTGT
    GCACTCCAAA GAGACATTCT TCTTCAGGGC CGACTCTACC TCTCCGAAAA CTGGATCTGC
    TTCTACAGCA ACATCTTCCG CTGGGAAACT CTGCTAACTG TGCGTTTGAA AGACATCTGC
    TCCATGACTA AAGAAAAGAC AGCTCGCCTC ATCCCCAATG CCATCCAAGT TTGCACTGAC
    TCAGAAAAGC ACTTTTTCAC TTCGTTCGGG GCCCGGGATC GGACGTACAT GATGATGTTC
    CGGCTCTGGC AGAATGCTCT CCTGGAAAAG CCCCTGTGCC CCAAGGAGCT GTGGCACTTC
    GTCCACCAGT GCTATGGGAA CGAGCTGGGC CTGACCAGTG ACGACGAGGA CTACGTGCCC
    CCCGATGACG ACTTCAACAC GATGGGCTAC TGTGAGGAGA TCCCCGTAGA AGAGAATGAA
    GTGAACGACA GCTCATCCAA AAGCAGCATA GAGACCAAGC CAGATGCCAG TCCACAGCTG
    CCCAAGAAAT CCATCACCAA CAGCACACTG ACGTCCACCG GGAGCAGTGA AGCCCCCGTC
    TCGTTTGACG GCCTGCCCCT GGAGGAGGAG GTGCTAGAGG GCGACGGGTC TCTGGAGAAG
    GAGCTCGCCA TCGACAACAT CATCGGGGAA AAGATTGAGA TCATCGCGCC CGTGAACTCC
    CCTTCGCTGG ACTTCAACGA CAACGAGGAC ATCCCCACTG AGCTCAGTGA CTCTTCAGAC
    ACCCACGACG AAGGGGAGGT CCAAGCCTTC TACGAGGACT TGAGTGGCCG GCAGTACGTG
    AACGAAGTCT TCAACTTCAG CGTGGACAAG CTCTATGACC TCCTGTTCAC TGACTCACCC
    TTCCAGCGGG ACTTCATGGA GCAGCGGCGC TTCTCAGATA TCATCTTCCA TCCGTGGAAG
    AAGGAAGAGA ATGGAAACCA GAGCCGAGTG ATCCTTTACA CCATCACCCT TACCAACCCT
    CTAGCTCCCA AAACTGCCAC CGTCAGGGAG ACACAGACCA TGTACAAGGC GAGCCAGGAG
    AGCGAGTGCT ATGTGATCGA CGCCGAGGTC CTCACGCACG ATGTACCGTA TCACGACTAC
    TTCTACACCA TCAACCGCTA CACGCTTACC CGCGTGGCCC GCAACAAGAG TCGACTCAGG
    GTCTCCACGG AGCTGCGCTA TCGGAAACAG CCCTGGGGGC TGGTGAAAAC ATTCATCGAG
    AAGAACTTCT GGAGTGGGCT GGAGGACTAC TTCCGCCACC TGGAGAGTGA GCTGACCAAA
    ACAGAGAGCA CCTACCTGGC GGAGATGCAC AGACAGTCTC CTAAAGAGAA GGCCAGCAAG
    CCCCCAACGG TGCGGAGGAG AAAACGGCCC CATGCCCACC TGCGGGTGCC ACACCTGGAG
    GAGGTGATGA GCCCCGTCAC CACGCCCACT GATGAGGACG TGGGCCACAG GATCAAACAT
    GTGGCAGGTT CCACACAGAC ACGGCACATC CCCGAGGACG GCCCCAGCGG TTTCCACCTG
    CAGAGCGTGT CCAAGCTGCT GCTGGTTATC AGCTGTGTTC TGGTGCTGCT GGTCATCCTC
    AACGTGATGC TCTTCTACAA ACTCTGGATG CTGGAGTACA CCACCCAGAC CCTCACTGCC
    TGGCAGGGTC TCCGGCTCCA AGAAAGGTTA CCCCAGTCCC AGACAGAGTG GGCCCAGCTC
    TTAGAGTCCC AGCAAAAGTA CCACGATACT GAGCTCCAGA AATGGAGGGA GATCATCAAA
    TCCTCGGTGA TGCTTCTTGA CCAGATGAAG GACTCACTCA TCAATCTTCA GAACGGCATC
    AGGTCCCGAG ACTACACGTC AGAAAGCGAA GAGAAGAGGA ACCGGTACCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178348.1
    CDS1..2634
    Translation

    Target ORF information:

    RefSeq Version NM_001191419.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191419.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA GCAGAGCAGC CAGCAGAGCA GCCATGACGA TGACTCCAGC
    AGGTTCCTGA GTCCTCGCGT GCGGGAAGAA AGCACTGCCA GTAACTCCAA CCGCAGCACG
    CCAGCCTGCT CCCCCATCCT CCGGAAGCGG TCTCGCTCAC CAACCCCGCA GAACGTAGAC
    GGAGACACCA TGGTGGAGAA GGGCTCAGAT CACTCCTCGG ACAAGTCGCC GTCCACTCCG
    GAGCAGGGCG TGCAGCGCAG CTGCTCCTCA CAGTCCGGCC GCAGTGGTGG CAAAAACTCC
    AAGAAAAGCC AGAGCTGGTA TAATGTGTTA AGCCCCACAT ACAAGCAGAG AAATGAAGAC
    TTCAGAAAGC TCTTTAAGCA ACTTCCAGAC ACGGAGCGCC TCATTGTTGA TTACTCCTGT
    GCACTCCAAA GAGACATTCT TCTTCAGGGC CGACTCTACC TCTCCGAAAA CTGGATCTGC
    TTCTACAGCA ACATCTTCCG CTGGGAAACT CTGCTAACTG TGCGTTTGAA AGACATCTGC
    TCCATGACTA AAGAAAAGAC AGCTCGCCTC ATCCCCAATG CCATCCAAGT TTGCACTGAC
    TCAGAAAAGC ACTTTTTCAC TTCGTTCGGG GCCCGGGATC GGACGTACAT GATGATGTTC
    CGGCTCTGGC AGAATGCTCT CCTGGAAAAG CCCCTGTGCC CCAAGGAGCT GTGGCACTTC
    GTCCACCAGT GCTATGGGAA CGAGCTGGGC CTGACCAGTG ACGACGAGGA CTACGTGCCC
    CCCGATGACG ACTTCAACAC GATGGGCTAC TGTGAGGAGA TCCCCGTAGA AGAGAATGAA
    GTGAACGACA GCTCATCCAA AAGCAGCATA GAGACCAAGC CAGATGCCAG TCCACAGCTG
    CCCAAGAAAT CCATCACCAA CAGCACACTG ACGTCCACCG GGAGCAGTGA AGCCCCCGTC
    TCGTTTGACG GCCTGCCCCT GGAGGAGGAG GTGCTAGAGG GCGACGGGTC TCTGGAGAAG
    GAGCTCGCCA TCGACAACAT CATCGGGGAA AAGATTGAGA TCATCGCGCC CGTGAACTCC
    CCTTCGCTGG ACTTCAACGA CAACGAGGAC ATCCCCACTG AGCTCAGTGA CTCTTCAGAC
    ACCCACGACG AAGGGGAGGT CCAAGCCTTC TACGAGGACT TGAGTGGCCG GCAGTACGTG
    AACGAAGTCT TCAACTTCAG CGTGGACAAG CTCTATGACC TCCTGTTCAC TGACTCACCC
    TTCCAGCGGG ACTTCATGGA GCAGCGGCGC TTCTCAGATA TCATCTTCCA TCCGTGGAAG
    AAGGAAGAGA ATGGAAACCA GAGCCGAGTG ATCCTTTACA CCATCACCCT TACCAACCCT
    CTAGCTCCCA AAACTGCCAC CGTCAGGGAG ACACAGACCA TGTACAAGGC GAGCCAGGAG
    AGCGAGTGCT ATGTGATCGA CGCCGAGGTC CTCACGCACG ATGTACCGTA TCACGACTAC
    TTCTACACCA TCAACCGCTA CACGCTTACC CGCGTGGCCC GCAACAAGAG TCGACTCAGG
    GTCTCCACGG AGCTGCGCTA TCGGAAACAG CCCTGGGGGC TGGTGAAAAC ATTCATCGAG
    AAGAACTTCT GGAGTGGGCT GGAGGACTAC TTCCGCCACC TGGAGAGTGA GCTGACCAAA
    ACAGAGAGCA CCTACCTGGC GGAGATGCAC AGACAGTCTC CTAAAGAGAA GGCCAGCAAG
    CCCCCAACGG TGCGGAGGAG AAAACGGCCC CATGCCCACC TGCGGGTGCC ACACCTGGAG
    GAGGTGATGA GCCCCGTCAC CACGCCCACT GATGAGGACG TGGGCCACAG GATCAAACAT
    GTGGCAGGTT CCACACAGAC ACGGCACATC CCCGAGGACG GCCCCAGCGG TTTCCACCTG
    CAGAGCGTGT CCAAGCTGCT GCTGGTTATC AGCTGTGTTC TGGTGCTGCT GGTCATCCTC
    AACGTGATGC TCTTCTACAA ACTCTGGATG CTGGAGTACA CCACCCAGAC CCTCACTGCC
    TGGCAGGGTC TCCGGCTCCA AGAAAGGTTA CCCCAGTCCC AGACAGAGTG GGCCCAGCTC
    TTAGAGTCCC AGCAAAAGTA CCACGATACT GAGCTCCAGA AATGGAGGGA GATCATCAAA
    TCCTCGGTGA TGCTTCTTGA CCAGATGAAG GACTCACTCA TCAATCTTCA GAACGGCATC
    AGGTCCCGAG ACTACACGTC AGAAAGCGAA GAGAAGAGGA ACCGGTACCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49670
    Clone ID Related Accession (Same CDS sequence) XM_010812449.2
    Accession Version XM_010812449.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA ACAGAGCAGC CAGCAGAGCA GCCAGCAGAG CAGCCATGAC
    GATGACTCCA GCAGGTTCCT GAGTCCTCGC GTGCGGGAAG AAAGCACTGC CAGTAACTCC
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGTCTCA CAAGCGCCTC TCAAAATACG ACAGACTTTA CCTGATCAAA
    AAAAGCCAGA GCTGGTATAA TGTGTTAAGC CCCACATACA AGCAGAGAAA TGAAGACTTC
    AGAAAGCTCT TTAAGCAACT TCCAGACACG GAGCGCCTCA TTGTTGATTA CTCCTGTGCA
    CTCCAAAGAG ACATTCTTCT TCAGGGCCGA CTCTACCTCT CCGAAAACTG GATCTGCTTC
    TACAGCAACA TCTTCCGCTG GGAAACTCTG CTAACTGTGC GTTTGAAAGA CATCTGCTCC
    ATGACTAAAG AAAAGACAGC TCGCCTCATC CCCAATGCCA TCCAAGTTTG CACTGACTCA
    GAAAAGCACT TTTTCACTTC GTTCGGGGCC CGGGATCGGA CGTACATGAT GATGTTCCGG
    CTCTGGCAGA ATGCTCTCCT GGAAAAGCCC CTGTGCCCCA AGGAGCTGTG GCACTTCGTC
    CACCAGTGCT ATGGGAACGA GCTGGGCCTG ACCAGTGACG ACGAGGACTA CGTGCCCCCC
    GATGACGACT TCAACACGAT GGGCTACTGT GAGGAGATCC CCGTAGAAGA GAATGAAGTG
    AACGACAGCT CATCCAAAAG CAGCATAGAG ACCAAGCCAG ATGCCAGTCC ACAGCTGCCC
    AAGAAATCCA TCACCAACAG CACACTGACG TCCACCGGGA GCAGTGAAGC CCCCGTCTCG
    TTTGACGGCC TGCCCCTGGA GGAGGAGGTG CTAGAGGGCG ACGGGTCTCT GGAGAAGGAG
    CTCGCCATCG ACAACATCAT CGGGGAAAAG ATTGAGATCA TCGCGCCCGT GAACTCCCCT
    TCGCTGGACT TCAACGACAA CGAGGACATC CCCACTGAGC TCAGTGACTC TTCAGACACC
    CACGACGAAG GGGAGGTCCA AGCCTTCTAC GAGGACTTGA GTGGCCGGCA GTACGTGAAC
    GAAGTCTTCA ACTTCAGCGT GGACAAGCTC TATGACCTCC TGTTCACTGA CTCACCCTTC
    CAGCGGGACT TCATGGAGCA GCGGCGCTTC TCAGATATCA TCTTCCATCC GTGGAAGAAG
    GAAGAGAATG GAAACCAGAG CCGAGTGATC CTTTACACCA TCACCCTTAC CAACCCTCTA
    GCTCCCAAAA CTGCCACCGT CAGGGAGACA CAGACCATGT ACAAGGCGAG CCAGGAGAGC
    GAGTGCTATG TGATCGACGC CGAGGTCCTC ACGCACGATG TACCGTATCA CGACTACTTC
    TACACCATCA ACCGCTACAC GCTTACCCGC GTGGCCCGCA ACAAGAGTCG ACTCAGGGTC
    TCCACGGAGC TGCGCTATCG GAAACAGCCC TGGGGGCTGG TGAAAACATT CATCGAGAAG
    AACTTCTGGA GTGGGCTGGA GGACTACTTC CGCCACCTGG AGAGTGAGCT GACCAAAACA
    GAGAGCACCT ACCTGGCGGA GATGCACAGA CAGTCTCCTA AAGAGAAGGC CAGCAAGCCC
    CCAACGGTGC GGAGGAGAAA ACGGCCCCAT GCCCACCTGC GGGTGCCACA CCTGGAGGAG
    GTGATGAGCC CCGTCACCAC GCCCACTGAT GAGGACGTGG GCCACAGGAT CAAACATGTG
    GCAGGTTCCA CACAGACACG GCACATCCCC GAGGACGGCC CCAGCGGTTT CCACCTGCAG
    AGCGTGTCCA AGCTGCTGCT GGTTATCAGC TGTGTTCTGG TGCTGCTGGT CATCCTCAAC
    GTGATGCTCT TCTACAAACT CTGGATGCTG GAGTACACCA CCCAGACCCT CACTGCCTGG
    CAGGGTCTCC GGCTCCAAGA AAGGTTACCC CAGTCCCAGA CAGAGTGGGC CCAGCTCTTA
    GAGTCCCAGC AAAAGTACCA CGATACTGAG CTCCAGAAAT GGAGGGAGAT CATCAAATCC
    TCGGTGATGC TTCTTGACCA GATGAAGGAC TCACTCATCA ATCTTCAGAA CGGCATCAGG
    TCCCGAGACT ACACGTCAGA AAGCGAAGAG AAGAGGAACC GGTACCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810751.1
    CDS30..2720
    Translation

    Target ORF information:

    RefSeq Version XM_010812449.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812449.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA ACAGAGCAGC CAGCAGAGCA GCCAGCAGAG CAGCCATGAC
    GATGACTCCA GCAGGTTCCT GAGTCCTCGC GTGCGGGAAG AAAGCACTGC CAGTAACTCC
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGTCTCA CAAGCGCCTC TCAAAATACG ACAGACTTTA CCTGATCAAA
    AAAAGCCAGA GCTGGTATAA TGTGTTAAGC CCCACATACA AGCAGAGAAA TGAAGACTTC
    AGAAAGCTCT TTAAGCAACT TCCAGACACG GAGCGCCTCA TTGTTGATTA CTCCTGTGCA
    CTCCAAAGAG ACATTCTTCT TCAGGGCCGA CTCTACCTCT CCGAAAACTG GATCTGCTTC
    TACAGCAACA TCTTCCGCTG GGAAACTCTG CTAACTGTGC GTTTGAAAGA CATCTGCTCC
    ATGACTAAAG AAAAGACAGC TCGCCTCATC CCCAATGCCA TCCAAGTTTG CACTGACTCA
    GAAAAGCACT TTTTCACTTC GTTCGGGGCC CGGGATCGGA CGTACATGAT GATGTTCCGG
    CTCTGGCAGA ATGCTCTCCT GGAAAAGCCC CTGTGCCCCA AGGAGCTGTG GCACTTCGTC
    CACCAGTGCT ATGGGAACGA GCTGGGCCTG ACCAGTGACG ACGAGGACTA CGTGCCCCCC
    GATGACGACT TCAACACGAT GGGCTACTGT GAGGAGATCC CCGTAGAAGA GAATGAAGTG
    AACGACAGCT CATCCAAAAG CAGCATAGAG ACCAAGCCAG ATGCCAGTCC ACAGCTGCCC
    AAGAAATCCA TCACCAACAG CACACTGACG TCCACCGGGA GCAGTGAAGC CCCCGTCTCG
    TTTGACGGCC TGCCCCTGGA GGAGGAGGTG CTAGAGGGCG ACGGGTCTCT GGAGAAGGAG
    CTCGCCATCG ACAACATCAT CGGGGAAAAG ATTGAGATCA TCGCGCCCGT GAACTCCCCT
    TCGCTGGACT TCAACGACAA CGAGGACATC CCCACTGAGC TCAGTGACTC TTCAGACACC
    CACGACGAAG GGGAGGTCCA AGCCTTCTAC GAGGACTTGA GTGGCCGGCA GTACGTGAAC
    GAAGTCTTCA ACTTCAGCGT GGACAAGCTC TATGACCTCC TGTTCACTGA CTCACCCTTC
    CAGCGGGACT TCATGGAGCA GCGGCGCTTC TCAGATATCA TCTTCCATCC GTGGAAGAAG
    GAAGAGAATG GAAACCAGAG CCGAGTGATC CTTTACACCA TCACCCTTAC CAACCCTCTA
    GCTCCCAAAA CTGCCACCGT CAGGGAGACA CAGACCATGT ACAAGGCGAG CCAGGAGAGC
    GAGTGCTATG TGATCGACGC CGAGGTCCTC ACGCACGATG TACCGTATCA CGACTACTTC
    TACACCATCA ACCGCTACAC GCTTACCCGC GTGGCCCGCA ACAAGAGTCG ACTCAGGGTC
    TCCACGGAGC TGCGCTATCG GAAACAGCCC TGGGGGCTGG TGAAAACATT CATCGAGAAG
    AACTTCTGGA GTGGGCTGGA GGACTACTTC CGCCACCTGG AGAGTGAGCT GACCAAAACA
    GAGAGCACCT ACCTGGCGGA GATGCACAGA CAGTCTCCTA AAGAGAAGGC CAGCAAGCCC
    CCAACGGTGC GGAGGAGAAA ACGGCCCCAT GCCCACCTGC GGGTGCCACA CCTGGAGGAG
    GTGATGAGCC CCGTCACCAC GCCCACTGAT GAGGACGTGG GCCACAGGAT CAAACATGTG
    GCAGGTTCCA CACAGACACG GCACATCCCC GAGGACGGCC CCAGCGGTTT CCACCTGCAG
    AGCGTGTCCA AGCTGCTGCT GGTTATCAGC TGTGTTCTGG TGCTGCTGGT CATCCTCAAC
    GTGATGCTCT TCTACAAACT CTGGATGCTG GAGTACACCA CCCAGACCCT CACTGCCTGG
    CAGGGTCTCC GGCTCCAAGA AAGGTTACCC CAGTCCCAGA CAGAGTGGGC CCAGCTCTTA
    GAGTCCCAGC AAAAGTACCA CGATACTGAG CTCCAGAAAT GGAGGGAGAT CATCAAATCC
    TCGGTGATGC TTCTTGACCA GATGAAGGAC TCACTCATCA ATCTTCAGAA CGGCATCAGG
    TCCCGAGACT ACACGTCAGA AAGCGAAGAG AAGAGGAACC GGTACCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49671
    Clone ID Related Accession (Same CDS sequence) XM_010812450.2
    Accession Version XM_010812450.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GRAM domain-containing protein 1B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA ACAGAGCAGC CAGCAGAGCA GCCAGCAGAG CAGCCATGAC
    GATGACTCCA GCAGGTTCCT GAGTCCTCGC GTGCGGGAAG AAAGCACTGC CAGTAACTCC
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGAAAAG CCAGAGCTGG TATAATGTGT TAAGCCCCAC ATACAAGCAG
    AGAAATGAAG ACTTCAGAAA GCTCTTTAAG CAACTTCCAG ACACGGAGCG CCTCATTGTT
    GATTACTCCT GTGCACTCCA AAGAGACATT CTTCTTCAGG GCCGACTCTA CCTCTCCGAA
    AACTGGATCT GCTTCTACAG CAACATCTTC CGCTGGGAAA CTCTGCTAAC TGTGCGTTTG
    AAAGACATCT GCTCCATGAC TAAAGAAAAG ACAGCTCGCC TCATCCCCAA TGCCATCCAA
    GTTTGCACTG ACTCAGAAAA GCACTTTTTC ACTTCGTTCG GGGCCCGGGA TCGGACGTAC
    ATGATGATGT TCCGGCTCTG GCAGAATGCT CTCCTGGAAA AGCCCCTGTG CCCCAAGGAG
    CTGTGGCACT TCGTCCACCA GTGCTATGGG AACGAGCTGG GCCTGACCAG TGACGACGAG
    GACTACGTGC CCCCCGATGA CGACTTCAAC ACGATGGGCT ACTGTGAGGA GATCCCCGTA
    GAAGAGAATG AAGTGAACGA CAGCTCATCC AAAAGCAGCA TAGAGACCAA GCCAGATGCC
    AGTCCACAGC TGCCCAAGAA ATCCATCACC AACAGCACAC TGACGTCCAC CGGGAGCAGT
    GAAGCCCCCG TCTCGTTTGA CGGCCTGCCC CTGGAGGAGG AGGTGCTAGA GGGCGACGGG
    TCTCTGGAGA AGGAGCTCGC CATCGACAAC ATCATCGGGG AAAAGATTGA GATCATCGCG
    CCCGTGAACT CCCCTTCGCT GGACTTCAAC GACAACGAGG ACATCCCCAC TGAGCTCAGT
    GACTCTTCAG ACACCCACGA CGAAGGGGAG GTCCAAGCCT TCTACGAGGA CTTGAGTGGC
    CGGCAGTACG TGAACGAAGT CTTCAACTTC AGCGTGGACA AGCTCTATGA CCTCCTGTTC
    ACTGACTCAC CCTTCCAGCG GGACTTCATG GAGCAGCGGC GCTTCTCAGA TATCATCTTC
    CATCCGTGGA AGAAGGAAGA GAATGGAAAC CAGAGCCGAG TGATCCTTTA CACCATCACC
    CTTACCAACC CTCTAGCTCC CAAAACTGCC ACCGTCAGGG AGACACAGAC CATGTACAAG
    GCGAGCCAGG AGAGCGAGTG CTATGTGATC GACGCCGAGG TCCTCACGCA CGATGTACCG
    TATCACGACT ACTTCTACAC CATCAACCGC TACACGCTTA CCCGCGTGGC CCGCAACAAG
    AGTCGACTCA GGGTCTCCAC GGAGCTGCGC TATCGGAAAC AGCCCTGGGG GCTGGTGAAA
    ACATTCATCG AGAAGAACTT CTGGAGTGGG CTGGAGGACT ACTTCCGCCA CCTGGAGAGT
    GAGCTGACCA AAACAGAGAG CACCTACCTG GCGGAGATGC ACAGACAGTC TCCTAAAGAG
    AAGGCCAGCA AGCCCCCAAC GGTGCGGAGG AGAAAACGGC CCCATGCCCA CCTGCGGGTG
    CCACACCTGG AGGAGGTGAT GAGCCCCGTC ACCACGCCCA CTGATGAGGA CGTGGGCCAC
    AGGATCAAAC ATGTGGCAGG TTCCACACAG ACACGGCACA TCCCCGAGGA CGGCCCCAGC
    GGTTTCCACC TGCAGAGCGT GTCCAAGCTG CTGCTGGTTA TCAGCTGTGT TCTGGTGCTG
    CTGGTCATCC TCAACGTGAT GCTCTTCTAC AAACTCTGGA TGCTGGAGTA CACCACCCAG
    ACCCTCACTG CCTGGCAGGG TCTCCGGCTC CAAGAAAGGT TACCCCAGTC CCAGACAGAG
    TGGGCCCAGC TCTTAGAGTC CCAGCAAAAG TACCACGATA CTGAGCTCCA GAAATGGAGG
    GAGATCATCA AATCCTCGGT GATGCTTCTT GACCAGATGA AGGACTCACT CATCAATCTT
    CAGAACGGCA TCAGGTCCCG AGACTACACG TCAGAAAGCG AAGAGAAGAG GAACCGGTAC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810752.1
    CDS29..2674
    Translation

    Target ORF information:

    RefSeq Version XM_010812450.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRAM domain containing 1B (GRAMD1B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812450.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGGCGG CCAACATGAT GGAGAATCTG CAGCCGCCCG CCCTGCAGGT GCCCGAGCCG 
    CGGGGCGCCC CCGAGGGCAG CCCGGTCTGG TCCAGCTCGT CGACCCCCAC GCTTCGCCGC
    CGGCGCTTCA AAATGCGCCG CACCAAGAAC GTGCAGGAGC CGAGCCTAGA GGCCGGGCTG
    AGCGGGGACC TGCCCGGCGT CTTGGCGCCC GGCAAGGAGT TCCTGCAGCT GCCATCCATC
    GAGATCACGC CCTCCAGCGA CGAGGACACC CCGTGGTCCA ATTGCTCCAC GCCCAGCGCT
    TCCCCGCGCC GCAAACGCTT CCTCCTGCGC AAGTGGCTGC GGGTGAGGGA GCGGAAGGAG
    TGCAGCGAAA GCAGCAGCCA ACAGAGCAGC CAGCAGAGCA GCCAGCAGAG CAGCCATGAC
    GATGACTCCA GCAGGTTCCT GAGTCCTCGC GTGCGGGAAG AAAGCACTGC CAGTAACTCC
    AACCGCAGCA CGCCAGCCTG CTCCCCCATC CTCCGGAAGC GGTCTCGCTC ACCAACCCCG
    CAGAACGTAG ACGGAGACAC CATGGTGGAG AAGGGCTCAG ATCACTCCTC GGACAAGTCG
    CCGTCCACTC CGGAGCAGGG CGTGCAGCGC AGCTGCTCCT CACAGTCCGG CCGCAGTGGT
    GGCAAAAACT CCAAGAAAAG CCAGAGCTGG TATAATGTGT TAAGCCCCAC ATACAAGCAG
    AGAAATGAAG ACTTCAGAAA GCTCTTTAAG CAACTTCCAG ACACGGAGCG CCTCATTGTT
    GATTACTCCT GTGCACTCCA AAGAGACATT CTTCTTCAGG GCCGACTCTA CCTCTCCGAA
    AACTGGATCT GCTTCTACAG CAACATCTTC CGCTGGGAAA CTCTGCTAAC TGTGCGTTTG
    AAAGACATCT GCTCCATGAC TAAAGAAAAG ACAGCTCGCC TCATCCCCAA TGCCATCCAA
    GTTTGCACTG ACTCAGAAAA GCACTTTTTC ACTTCGTTCG GGGCCCGGGA TCGGACGTAC
    ATGATGATGT TCCGGCTCTG GCAGAATGCT CTCCTGGAAA AGCCCCTGTG CCCCAAGGAG
    CTGTGGCACT TCGTCCACCA GTGCTATGGG AACGAGCTGG GCCTGACCAG TGACGACGAG
    GACTACGTGC CCCCCGATGA CGACTTCAAC ACGATGGGCT ACTGTGAGGA GATCCCCGTA
    GAAGAGAATG AAGTGAACGA CAGCTCATCC AAAAGCAGCA TAGAGACCAA GCCAGATGCC
    AGTCCACAGC TGCCCAAGAA ATCCATCACC AACAGCACAC TGACGTCCAC CGGGAGCAGT
    GAAGCCCCCG TCTCGTTTGA CGGCCTGCCC CTGGAGGAGG AGGTGCTAGA GGGCGACGGG
    TCTCTGGAGA AGGAGCTCGC CATCGACAAC ATCATCGGGG AAAAGATTGA GATCATCGCG
    CCCGTGAACT CCCCTTCGCT GGACTTCAAC GACAACGAGG ACATCCCCAC TGAGCTCAGT
    GACTCTTCAG ACACCCACGA CGAAGGGGAG GTCCAAGCCT TCTACGAGGA CTTGAGTGGC
    CGGCAGTACG TGAACGAAGT CTTCAACTTC AGCGTGGACA AGCTCTATGA CCTCCTGTTC
    ACTGACTCAC CCTTCCAGCG GGACTTCATG GAGCAGCGGC GCTTCTCAGA TATCATCTTC
    CATCCGTGGA AGAAGGAAGA GAATGGAAAC CAGAGCCGAG TGATCCTTTA CACCATCACC
    CTTACCAACC CTCTAGCTCC CAAAACTGCC ACCGTCAGGG AGACACAGAC CATGTACAAG
    GCGAGCCAGG AGAGCGAGTG CTATGTGATC GACGCCGAGG TCCTCACGCA CGATGTACCG
    TATCACGACT ACTTCTACAC CATCAACCGC TACACGCTTA CCCGCGTGGC CCGCAACAAG
    AGTCGACTCA GGGTCTCCAC GGAGCTGCGC TATCGGAAAC AGCCCTGGGG GCTGGTGAAA
    ACATTCATCG AGAAGAACTT CTGGAGTGGG CTGGAGGACT ACTTCCGCCA CCTGGAGAGT
    GAGCTGACCA AAACAGAGAG CACCTACCTG GCGGAGATGC ACAGACAGTC TCCTAAAGAG
    AAGGCCAGCA AGCCCCCAAC GGTGCGGAGG AGAAAACGGC CCCATGCCCA CCTGCGGGTG
    CCACACCTGG AGGAGGTGAT GAGCCCCGTC ACCACGCCCA CTGATGAGGA CGTGGGCCAC
    AGGATCAAAC ATGTGGCAGG TTCCACACAG ACACGGCACA TCCCCGAGGA CGGCCCCAGC
    GGTTTCCACC TGCAGAGCGT GTCCAAGCTG CTGCTGGTTA TCAGCTGTGT TCTGGTGCTG
    CTGGTCATCC TCAACGTGAT GCTCTTCTAC AAACTCTGGA TGCTGGAGTA CACCACCCAG
    ACCCTCACTG CCTGGCAGGG TCTCCGGCTC CAAGAAAGGT TACCCCAGTC CCAGACAGAG
    TGGGCCCAGC TCTTAGAGTC CCAGCAAAAG TACCACGATA CTGAGCTCCA GAAATGGAGG
    GAGATCATCA AATCCTCGGT GATGCTTCTT GACCAGATGA AGGACTCACT CATCAATCTT
    CAGAACGGCA TCAGGTCCCG AGACTACACG TCAGAAAGCG AAGAGAAGAG GAACCGGTAC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.