ncapg2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ncapg2
Entrez Gene ID	100495586
Full Name	non-SMC condensin II complex subunit G2
General protein information	Preferred Names non-SMC condensin II complex subunit G2 Names condensin-2 complex subunit G2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935173.5	XP_002935219.2	condensin-2 complex subunit G2
XM_002935173.4	XP_002935219.1	condensin-2 complex subunit G2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NCAPG2	XP_005629775.1
Bos taurus (cattle)	NCAPG2	NP_001179378.1
Rattus norvegicus (Norway rat)	Ncapg2	XP_006240770.1
Xenopus tropicalis (tropical clawed frog)	ncapg2	XP_002935219.1
Danio rerio (zebrafish)	ncapg2	NP_001082961.2
Homo sapiens (human)	NCAPG2	NP_060230.5
	NCAPG2	XP_001083395.1
Mus musculus (house mouse)	Ncapg2	NP_598523.3
Gallus gallus (chicken)	LOC100859557	XP_003640726.1

Gene Ontology

The following ncapg2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ncapg2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa60186	XM_002935173.5 Latest version!	Xenopus tropicalis non-SMC condensin II complex subunit G2 (ncapg2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa12121	XM_002935173.4 Latest version!	Xenopus tropicalis non-SMC condensin II complex subunit G2 (ncapg2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ncapg2 ( XM_002935173.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935219.2 Xenopus tropicalis non-SMC condensin II complex subunit G2 (ncapg2), mRNA.

Price & Availability↑

CloneID	OXa60186
Clone ID Related Accession (Same CDS sequence)	XM_002935173.5
Accession Version	XM_002935173.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	condensin-2 complex subunit G2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935173.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC GTGAGGCATT CTTGCAAGCC ACTGCCAAGG ATTCTGTAGA AGATTTCCTG 
AACTTCATAC AGCTTCATAA AGACGTGTCT GATCCTTTTG ATCTAAATGA ATTACTGCAA 
GAGTTGCCAA GGAAACAGAA GGAGGAATTA TGGGAGAAAC TAAAGACTCT TTTAACTGAT 
ACTTTGGTGG CAAACCCTGT GGAAGGGTGG CAAAATATTG ATGATGACAG TGATGATGAC 
ATGGAAGTAG AGAGTTCCTC AGATGTGAAA CAAACAATGT CTATAATCCA CGGGCTAACA 
ATAGCTGCTG CAGCTTCTGT GTGTGTCATA GATGAAGATG TGTGCTATGA AGCTCTCTTA 
GAATGTGCAG CAATCCTCAG TGGTATTGTT CATGCTTTGC CTAAATCCGA ATCCCACATT 
ATCCTTGCTA TCAGACATCT ATGTGAAGCC TGGTGGGAAA AAGGGCTCCA AGGAAAAGAA 
GAATTTGGGA AAACCGCCTT TCTTTTGCTT TTGGCAAAAT CTCTTGAAGT AAAGTGTGTG 
GTTGCTGATA TTGGGCGCTT GTGGCATCTG CATCCAGCTC TGCTCAGTTT TGATTTCAAC 
TCTGAAGAGA GTCACAACGT GAAGGATCTG CTGCTCCAGT GCTTCCTGAG CATTAACCAT 
ATTAAGAGAG AGGAGGGCAG AAGATTTCTT AGCTTTCTTT TCAGCTGGGA TGCCAGCTTC 
ATTAAAATGA TCCATGGGAC TATCAAAAAT CAGCTTCAGT GCTTACCAAA ATCGTTGATG 
ACCCACATCG CAGACATTTA CTTTAGGGCC TGGAAGAAAG CCTCGGGGGA TGTTTTACAG 
ATGATTGAGA ACAGCTGTAT TCAAGATTTT ATGCATCATG GAGTCCATCT ACCAAGGAAT 
TCTCCACTTC ACCCCAAAGT GCGAGAGGTG CTAAGTTACT TTCACCAACA GAAGCTACGT 
CAGGGTGTGG AAGAAATGCT TTGTAGACTG TATCAGCCCA TTATTTGGCG AGGCCTAAAG 
GCAAGGAATT CTGAAGTTCG GTCAAATGCT GCCCTGTTAT TTGTCGAAGC ATTTCCTATA 
AGGGACCCCA ATCTTAACCA TGAGGATATG GACAATGAAA TCCAGAAACA GTTTGAAGAA 
CTTTTTAATC TTTTGGAGGA TCCCCAGCCT CTTGTCCGTT CTACAGGGGT TTTAGGTGTC 
TGTAAGATAA CTGCTAAGTA CTGGGAAATG ATTCCTCCAG CCATTCTGAC TGATCTTCTG 
CGGAAAATAC TTGGCGATCT AGCTGCGGAT GTAAGCTCGG CCGATGTTCG TTGTTCTGTA 
TTTAAGTGTT TACCGATTCT GTTGGACAAC AAGTTAAGTC ATCCGCTGCT GGAGAAGATG 
CTGCCGGCAC TGAAATTCTG TCTCCATGAC AATTCTGAGA AAGTTCGAGT GGCTTTTGTT 
GATATGCTGC TGAAGATCAA AGCTGTCAAA GCGGCCAAGT TCTGGAAGAT CTGCCCTATG 
GAACAGATTT TGGCACGCCT TGAAGTGGAT TCCCGCCCTG TTTCTCGACG AATTGTTAAC 
CTTCTGTTTA ACTCTTTCTT TCCTGTCAAT CAACAAGAGG AGGTTTGGTG CGAGCGCTGT 
GTTGCTCTCA TCCAAATGAA CCCAGCCGCA GCACGAAAGT TTTATCAATA CGCATACGAG 
CATACTGCCC CCACCAATAT AGCTAAATTG ATGCTGACTA TACGCCGTTG TTTAAATGCC 
TGCATTCAGC GGACTGTTCG AAACGAAGAT AGTGAGGATG AGGAGGATGA CGAGGAAATT 
GTCAGAGGCG ATAATGAAAA GGAAAATAAA AGTGTTTTGG AGAATGTGTT ATCCACGGAT 
GATTCTTCCT CTATGGCGAG TTTGCTGGAG ATTGTTGTTG TTCTCTGGCG CAGTATCAGA 
AAAGCCCTTG AACAGAATGA AGAAGCCAAA ACCTACACCA TCAGCAAATT TGCTACAGTG 
CTTCCAGAAT ATTTCAAAGT ATTTAGGGAT GATCGCTGTA CTGTTCCGTT AATTATTCTG 
GCTTCCTTCA TGCCTCCATC TGCTGTCCCA ACTTTCAGCT GCTCTGTCTT ATCTAAGCTG 
AGACACTTGG ACGATGGCGC AGATGAGCAC AAATATTCCA CTCTTATTGA CTGTCTATGC 
CGCTGGGGGC AAGTGGGGCA TGTATTGGAG CTGGCAACCG AATGGCTCTC AGAGTCATAC 
CCAGAGAAAA GGGGGAGAAA AGATTCCAAT CGGCAAGTGC GTATCCAGGA CACAGTGGAA 
TCAAAGCCTT CGCTGGCCCT TGATTATATA GAATATATAG TTACCCACAC CATGAATCGA 
GATTGCCTTT TGTCCCTTCA AACAAAGAAA CTGAACCAAC TCCTGAAAGT GCTTGGTTTA 
GTAAAGGAGG TGCTGTTCTG CTACATGAAG CCATCCGAAG CAGTGACACA TAATATCAAC 
CAAGATACAG CATTAAGAGC CTTCAGCCTG TACTGCAGAC TCAGCATCCA CCTGCAACAT 
AAGTTTAGCT CTGAAGGAAG AACATATTTG TCTTTGCTGG AAGACACAGG AGGGTGGATC 
GAGAGCCAAG TCCTTCCAAC TCTAGAAAGT AATGGAGACC TCTCCGAGGA GTCATGTAAT 
ATGTGCCATC AGATCCTCAA GGCCTATCTG ACTGTTTGTA AAGACGTATT GATGGTTGGC 
CTCGCTGACT CGGAATTTCA AGCCCAGCTT CTTCAGATTA CTCTTTCAGT GATACAGACT 
GAGAAGTGCC ATGATTGTTT GCCAATGCTG TTCTCTGTGC TGAAGGAAAT CACAGAACTG 
TGTTTGGCTC ACAAGATGAG CGACGCCAGC GTGGAATGCG ACGAGATGCT CGATGCCATC 
CAGAGAGTAT TCCATAAGTC TTTGGAGACT GTCGCACGCG GGCTTAGAAA ACAGAGGGAA 
GAGGCACTAC CGCTTCTGCA AGCTATTCAG CCTTCTCTAG GAGAGTTTGT TCACACAGTT 
CAGTGCTGGC ACACCGCTTC CAAGGTGGTA CATCGAGGGA TGCTTTCCAC GCTGTTGGCT 
GCAGTGGTTG TTGAAATAAG CCACTCACTG CGCAAAATTA CAGATTTAAG TGAACTAACG 
CCACCCACAT CTATTTCTGA TCTGCCTCCG CTTTCAAAAT GTATAATGAC AATTATCGTT 
AAATCTCCAA GTGCTGTAAG TTCATTCTTG GATGAATTGA CAGAATGCAT CACATTAGAA 
GAAGTAGAAG GAATACTGAG CTTGTCCGCA TCTTTGTATG TTGCTGTTGT CTGCAATAAA 
AGGAAACAAA TACCCCCTGC AGTTAAGAAT ACTGCATCAG CAATTTATAG GAAACTGAAG 
AATTTCAGTG AGGTAACCAT GGACGATGCC GGAAGCATTG AAAGGGCTAT ATATGAATCA 
TCCATGAGGA TATTAGATGA AATGCTGCAT CCATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935219.2
CDS	118..3576
Translation	MSKREAFLQATAKDSVEDFLNFIQLHKDVSDPFDLNELLQELPRKQKEELWEKLKTLLTDTLVANPVEGWQNIDDDSDDDMEVESSSDVKQTMSIIHGLTIAAAASVCVIDEDVCYEALLECAAILSGIVHALPKSESHIILAIRHLCEAWWEKGLQGKEEFGKTAFLLLLAKSLEVKCVVADIGRLWHLHPALLSFDFNSEESHNVKDLLLQCFLSINHIKREEGRRFLSFLFSWDASFIKMIHGTIKNQLQCLPKSLMTHIADIYFRAWKKASGDVLQMIENSCIQDFMHHGVHLPRNSPLHPKVREVLSYFHQQKLRQGVEEMLCRLYQPIIWRGLKARNSEVRSNAALLFVEAFPIRDPNLNHEDMDNEIQKQFEELFNLLEDPQPLVRSTGVLGVCKITAKYWEMIPPAILTDLLRKILGDLAADVSSADVRCSVFKCLPILLDNKLSHPLLEKMLPALKFCLHDNSEKVRVAFVDMLLKIKAVKAAKFWKICPMEQILARLEVDSRPVSRRIVNLLFNSFFPVNQQEEVWCERCVALIQMNPAAARKFYQYAYEHTAPTNIAKLMLTIRRCLNACIQRTVRNEDSEDEEDDEEIVRGDNEKENKSVLENVLSTDDSSSMASLLEIVVVLWRSIRKALEQNEEAKTYTISKFATVLPEYFKVFRDDRCTVPLIILASFMPPSAVPTFSCSVLSKLRHLDDGADEHKYSTLIDCLCRWGQVGHVLELATEWLSESYPEKRGRKDSNRQVRIQDTVESKPSLALDYIEYIVTHTMNRDCLLSLQTKKLNQLLKVLGLVKEVLFCYMKPSEAVTHNINQDTALRAFSLYCRLSIHLQHKFSSEGRTYLSLLEDTGGWIESQVLPTLESNGDLSEESCNMCHQILKAYLTVCKDVLMVGLADSEFQAQLLQITLSVIQTEKCHDCLPMLFSVLKEITELCLAHKMSDASVECDEMLDAIQRVFHKSLETVARGLRKQREEALPLLQAIQPSLGEFVHTVQCWHTASKVVHRGMLSTLLAAVVVEISHSLRKITDLSELTPPTSISDLPPLSKCIMTIIVKSPSAVSSFLDELTECITLEEVEGILSLSASLYVAVVCNKRKQIPPAVKNTASAIYRKLKNFSEVTMDDAGSIERAIYESSMRILDEMLHPS

Target ORF information:

RefSeq Version	XM_002935173.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis non-SMC condensin II complex subunit G2 (ncapg2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935173.5

ORF Insert Sequence:

ATGTCCAAGC GTGAGGCATT CTTGCAAGCC ACTGCCAAGG ATTCTGTAGA AGATTTCCTG 
AACTTCATAC AGCTTCATAA AGACGTGTCT GATCCTTTTG ATCTAAATGA ATTACTGCAA 
GAGTTGCCAA GGAAACAGAA GGAGGAATTA TGGGAGAAAC TAAAGACTCT TTTAACTGAT 
ACTTTGGTGG CAAACCCTGT GGAAGGGTGG CAAAATATTG ATGATGACAG TGATGATGAC 
ATGGAAGTAG AGAGTTCCTC AGATGTGAAA CAAACAATGT CTATAATCCA CGGGCTAACA 
ATAGCTGCTG CAGCTTCTGT GTGTGTCATA GATGAAGATG TGTGCTATGA AGCTCTCTTA 
GAATGTGCAG CAATCCTCAG TGGTATTGTT CATGCTTTGC CTAAATCCGA ATCCCACATT 
ATCCTTGCTA TCAGACATCT ATGTGAAGCC TGGTGGGAAA AAGGGCTCCA AGGAAAAGAA 
GAATTTGGGA AAACCGCCTT TCTTTTGCTT TTGGCAAAAT CTCTTGAAGT AAAGTGTGTG 
GTTGCTGATA TTGGGCGCTT GTGGCATCTG CATCCAGCTC TGCTCAGTTT TGATTTCAAC 
TCTGAAGAGA GTCACAACGT GAAGGATCTG CTGCTCCAGT GCTTCCTGAG CATTAACCAT 
ATTAAGAGAG AGGAGGGCAG AAGATTTCTT AGCTTTCTTT TCAGCTGGGA TGCCAGCTTC 
ATTAAAATGA TCCATGGGAC TATCAAAAAT CAGCTTCAGT GCTTACCAAA ATCGTTGATG 
ACCCACATCG CAGACATTTA CTTTAGGGCC TGGAAGAAAG CCTCGGGGGA TGTTTTACAG 
ATGATTGAGA ACAGCTGTAT TCAAGATTTT ATGCATCATG GAGTCCATCT ACCAAGGAAT 
TCTCCACTTC ACCCCAAAGT GCGAGAGGTG CTAAGTTACT TTCACCAACA GAAGCTACGT 
CAGGGTGTGG AAGAAATGCT TTGTAGACTG TATCAGCCCA TTATTTGGCG AGGCCTAAAG 
GCAAGGAATT CTGAAGTTCG GTCAAATGCT GCCCTGTTAT TTGTCGAAGC ATTTCCTATA 
AGGGACCCCA ATCTTAACCA TGAGGATATG GACAATGAAA TCCAGAAACA GTTTGAAGAA 
CTTTTTAATC TTTTGGAGGA TCCCCAGCCT CTTGTCCGTT CTACAGGGGT TTTAGGTGTC 
TGTAAGATAA CTGCTAAGTA CTGGGAAATG ATTCCTCCAG CCATTCTGAC TGATCTTCTG 
CGGAAAATAC TTGGCGATCT AGCTGCGGAT GTAAGCTCGG CCGATGTTCG TTGTTCTGTA 
TTTAAGTGTT TACCGATTCT GTTGGACAAC AAGTTAAGTC ATCCGCTGCT GGAGAAGATG 
CTGCCGGCAC TGAAATTCTG TCTCCATGAC AATTCTGAGA AAGTTCGAGT GGCTTTTGTT 
GATATGCTGC TGAAGATCAA AGCTGTCAAA GCGGCCAAGT TCTGGAAGAT CTGCCCTATG 
GAACAGATTT TGGCACGCCT TGAAGTGGAT TCCCGCCCTG TTTCTCGACG AATTGTTAAC 
CTTCTGTTTA ACTCTTTCTT TCCTGTCAAT CAACAAGAGG AGGTTTGGTG CGAGCGCTGT 
GTTGCTCTCA TCCAAATGAA CCCAGCCGCA GCACGAAAGT TTTATCAATA CGCATACGAG 
CATACTGCCC CCACCAATAT AGCTAAATTG ATGCTGACTA TACGCCGTTG TTTAAATGCC 
TGCATTCAGC GGACTGTTCG AAACGAAGAT AGTGAGGATG AGGAGGATGA CGAGGAAATT 
GTCAGAGGCG ATAATGAAAA GGAAAATAAA AGTGTTTTGG AGAATGTGTT ATCCACGGAT 
GATTCTTCCT CTATGGCGAG TTTGCTGGAG ATTGTTGTTG TTCTCTGGCG CAGTATCAGA 
AAAGCCCTTG AACAGAATGA AGAAGCCAAA ACCTACACCA TCAGCAAATT TGCTACAGTG 
CTTCCAGAAT ATTTCAAAGT ATTTAGGGAT GATCGCTGTA CTGTTCCGTT AATTATTCTG 
GCTTCCTTCA TGCCTCCATC TGCTGTCCCA ACTTTCAGCT GCTCTGTCTT ATCTAAGCTG 
AGACACTTGG ACGATGGCGC AGATGAGCAC AAATATTCCA CTCTTATTGA CTGTCTATGC 
CGCTGGGGGC AAGTGGGGCA TGTATTGGAG CTGGCAACCG AATGGCTCTC AGAGTCATAC 
CCAGAGAAAA GGGGGAGAAA AGATTCCAAT CGGCAAGTGC GTATCCAGGA CACAGTGGAA 
TCAAAGCCTT CGCTGGCCCT TGATTATATA GAATATATAG TTACCCACAC CATGAATCGA 
GATTGCCTTT TGTCCCTTCA AACAAAGAAA CTGAACCAAC TCCTGAAAGT GCTTGGTTTA 
GTAAAGGAGG TGCTGTTCTG CTACATGAAG CCATCCGAAG CAGTGACACA TAATATCAAC 
CAAGATACAG CATTAAGAGC CTTCAGCCTG TACTGCAGAC TCAGCATCCA CCTGCAACAT 
AAGTTTAGCT CTGAAGGAAG AACATATTTG TCTTTGCTGG AAGACACAGG AGGGTGGATC 
GAGAGCCAAG TCCTTCCAAC TCTAGAAAGT AATGGAGACC TCTCCGAGGA GTCATGTAAT 
ATGTGCCATC AGATCCTCAA GGCCTATCTG ACTGTTTGTA AAGACGTATT GATGGTTGGC 
CTCGCTGACT CGGAATTTCA AGCCCAGCTT CTTCAGATTA CTCTTTCAGT GATACAGACT 
GAGAAGTGCC ATGATTGTTT GCCAATGCTG TTCTCTGTGC TGAAGGAAAT CACAGAACTG 
TGTTTGGCTC ACAAGATGAG CGACGCCAGC GTGGAATGCG ACGAGATGCT CGATGCCATC 
CAGAGAGTAT TCCATAAGTC TTTGGAGACT GTCGCACGCG GGCTTAGAAA ACAGAGGGAA 
GAGGCACTAC CGCTTCTGCA AGCTATTCAG CCTTCTCTAG GAGAGTTTGT TCACACAGTT 
CAGTGCTGGC ACACCGCTTC CAAGGTGGTA CATCGAGGGA TGCTTTCCAC GCTGTTGGCT 
GCAGTGGTTG TTGAAATAAG CCACTCACTG CGCAAAATTA CAGATTTAAG TGAACTAACG 
CCACCCACAT CTATTTCTGA TCTGCCTCCG CTTTCAAAAT GTATAATGAC AATTATCGTT 
AAATCTCCAA GTGCTGTAAG TTCATTCTTG GATGAATTGA CAGAATGCAT CACATTAGAA 
GAAGTAGAAG GAATACTGAG CTTGTCCGCA TCTTTGTATG TTGCTGTTGT CTGCAATAAA 
AGGAAACAAA TACCCCCTGC AGTTAAGAAT ACTGCATCAG CAATTTATAG GAAACTGAAG 
AATTTCAGTG AGGTAACCAT GGACGATGCC GGAAGCATTG AAAGGGCTAT ATATGAATCA 
TCCATGAGGA TATTAGATGA AATGCTGCAT CCATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncapg2 ( XM_002935173.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935219.1 Xenopus tropicalis non-SMC condensin II complex subunit G2 (ncapg2), mRNA.

Price & Availability↑

CloneID	OXa12121
Clone ID Related Accession (Same CDS sequence)	XM_002935173.4
Accession Version	XM_002935173.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	condensin-2 complex subunit G2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935173.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC GTGAGGCATT CTTGCAAGCC ACTGCCAAGG ATTCTGTAGA AGATTTCCTG 
AACTTCATAC AGCTTCATAA AGACGTGTCT GATCCTTTTG ATCTAAATGA ATTACTGCAA 
GAGTTGCCAA GGAAACAGAA GGAGGAATTA TGGGAGAAAC TAAAGACTCT TTTAACTGAT 
ACTTTGGTGG AAAACCCTGT GGAAGGGTGG CAAAATATTG ATGATGACAG TGATGATGAC 
ATGGAAGTAG AGAGTTCCTC AGATGTGAAA CAAACAATGT CTATAATCCA CGGGCTAACA 
ATAGCTGCTG CAGCTTCTGT GTGTGTCATA GATGAAGATG TGTGCTATGA AGCTCTCTTA 
GAATGTGCAG CAATCCTCAG TGGTATTGTT CATGCTTTGC CTAAATCCGA ATCCCACATT 
ATCCTTGCTA TCAGACATCT ATGTGAAGCC TGGTGGGAAA AAGGGCTCCA AGGAAAAGAA 
GAATTTGGGA AAACCGCCTT TCTTTTGCTT TTGGCAAAAT CTCTTGAAGT AAAGTGTGTG 
GTTGCTGATA TTGGGCGCTT GTGGCATCTG CATCCAGCTC TGCTCAGTTT TGATTTCAAC 
TCTGAAGAGA GTCACAACGT GAAGGATCTG CTGCTCCAGT GCTTCCTGAG CATTAACCAT 
ATTAAGAGAG AGGAGGGCAG AAGATTTCTT AGCTTTCTTT TCAGCTGGGA TGCCAGCTTC 
ATTAAAATGA TCCATGGGAC TATCAAAAAT CAGCTTCAGT GCTTACCAAA ATCGTTGATG 
ACCCACATCG CAGACATTTA CTTTAGGGCC TGGAAGAAAG CCTCGGGGGA TGTTTTACAG 
ATGATTGAGA ACAGCTGTAT TCAAGATTTT ATGCATCATG GAGTCCATCT ACCAAGGAAT 
TCTCCACTTC ACCCCAAAGT GCGAGAGGTG CTAAGTTACT TTCACCAACA GAAGCTACGT 
CAGGGTGTGG AAGAAATGCT TTGTAGACTG TATCAGCCCA TTATTTGGCG AGGCCTAAAG 
GCAAGGAATT CTGAAGTTCG GTCAAATGCT GCCCTGTTAT TTGTCGAAGC ATTTCCTATA 
AGGGACCCCA ATCTTAACCA TGAGGATATG GACAATGAAA TCCAGAAACA GTTTGAAGAA 
CTTTTTAATC TTTTGGAGGA TCCCCAGCCT CTTGTCCGTT CTACAGGGGT TTTAGGTGTC 
TGTAAGATAA CTGCTAAGTA CTGGGAAATG ATTCCTCCAG CCATTCTGAC TGATCTTCTG 
CGGAAAATAC TTGGCGATCT AGCTGCGGAT GTAAGCTCGG CCGATGTTCG TTGTTCTGTA 
TTTAAGTGTT TACCGATTCT GTTGGACAAC AAGTTAAGTC ATCCGCTGCT GGAGAAGATG 
CTGCCGGCAC TGAAATTCTG TCTCCATGAC AATTCTGAGA AAGTTCGAGT GGCTTTTGTT 
GATATGCTGC TGAAGATCAA AGCTGTCAAA GCGGCCAAGT TCTGGAAGAT CTGCCCTATG 
GAACAGATTT TGGCACGCCT TGAAGTGGAT TCCCGCCCTG TTTCTCGACG AATTGTTAAC 
CTTCTGTTTA ACTCTTTCTT TCCTGTCAAT CAACAAGAGG AGGTTTGGTG CGAGCGCTGT 
GTTGCTCTCA TCCAAATGAA CCCAGCCGCA GCACGAAAGT TTTATCAATA CGCATACGAG 
CATACTGCCC CCACCAATAT AGCTAAATTG ATGCTGACTA TACGCCGTTG TTTAAATGCC 
TGCATTCAGC GGACTGTTCG AAACGAAGAT AGTGAGGATG AGGAGGATGA CGAGGAAATT 
GTCAGAGGCG ATAATGAAAA GGAAAATAAA AGTGTTTTGG AGAATGTGTT ATCCACGGAT 
GATTCTTCCT CTATGGCGAG TTTGCTGGAG ATTGTTGTGG TTCTCTGGCG CAGTATCAGA 
AAAGCCCTTG AACAGAATGA AGAAGCCAAA ACCTACACCA TCAGCAAATT TGCTACAGTG 
CTTCCAGAAT ATTTCAAAGT ATTTAGGGAT GATCGCTGTA CTGTTCCGTT AATTATTCTG 
GCTTCCTTCA TGCCTCCATC TGCTGTCCCA ACTTTCAGCT GCTCTGTCTT ATCTAAGCTG 
AGACACTTGG ACGATGGCGC AGATGAGCAC AAATATTCCA CTCTTATTGA CTGTCTATGC 
CGCTGGGGGC AAGTGGGGCA TGTATTGGAG CTGGCAACCG AATGGCTCTC AGAGTCATAC 
CCAGAGAAAA GGGGGAGAAA AGATTCCAAT CGGCAAGTGC GTATCCAGGA CACAGTGGAA 
TCAAAGCCTT CGCTGGCCCT TGATTATATA GAATATATAG TTACCCACAC CATGAATCGA 
GATTGCCTTT TGTCCCTTCA AACAAAGAAA CTGAACCAAC TCCTGAAAGT GCTTGGTTTA 
GTAAAGGAGG TGCTGTTCTG CTACATGAAG CCATCCGAAG CAGTGACACA TAATATCAAC 
CAAGATACAG CATTAAGAGC CTTCAGCCTG TACTGCAGAC TCAGCATCCA CCTGCAACAT 
AAGTTTAGCT CTGAAGGAAG AACATATTTG TCTTTGCTGG AAGACACAGG AGGGTGGATC 
GAGAGCCAAG TCCTTCCAAC TCTAGAAAGT AATGGAGACC TCTCCGAGGA GTCATGTAAT 
ATGTGCCATC AGATCCTCAA GGCCTATCTG ACTGTTTGTA AAGACGTATT GATGGTTGGC 
CTCGCTGACT CGGAATTTCA AGCCCAGCTT CTTCAGATTA CTCTTTCAGT GATACAGACT 
GAGAAGTGCC ATGATTGTTT GCCAATGCTG TTCTCTGTGC TGAAGGAAAT CACAGAACTG 
TGTTTGGCTC ACAAGATGAG CGACGCCAGC GTGGAATGCG ACGAGATGCT CGATGCCATC 
CAGAGAGTAT TCCATAAGTC TTTGGAGACT GTCGCACGCG GGCTTAGAAA ACAGAGGGAA 
GAGGCACTAC CGCTTCTGCA AGCTATTCAG CCTTCTCTAG GAGAGTTTGT CCACACAGTT 
CAGTGCTGGC ACACCGCTTC CAAGGTGGTA CATCGAGGGA TGCTTTCCAC GCTGTTGGCT 
GCAGTGGTTG TTGAAATAAG CCACTCACTG CGCAAAATTA CAGATTTAAG TGAACTAACG 
CCACCCACAT CTATTTCTGA TCTGCCTCCG CTTTCAAAAT GTATAATGAC AATTATCGTT 
AAATCTCCAA GTGCTGTAAG TTCATTCTTG GATGAATTGA CAGAATGCAT CACATTAGAA 
GAAGTAGAAG GAATACTGAG CTTGTCCGCA TCTTTGTATG TTGCTGTTGT CTGCAATAAA 
AGGAAACAAA TACCCCCTGC AGTTAAGAAT ACTGCATCAG CAATTTATAG GAAACTGAAG 
AATTTCAGTG AGGTAACCAT GGACGATGCC GGAAGCATTG AAAGGGCTAT ATATGAATCA 
TCCATGAGGA TATTAGATGA AATGCTGCAT CCATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935219.1
CDS	119..3577
Translation	MSKREAFLQATAKDSVEDFLNFIQLHKDVSDPFDLNELLQELPRKQKEELWEKLKTLLTDTLVENPVEGWQNIDDDSDDDMEVESSSDVKQTMSIIHGLTIAAAASVCVIDEDVCYEALLECAAILSGIVHALPKSESHIILAIRHLCEAWWEKGLQGKEEFGKTAFLLLLAKSLEVKCVVADIGRLWHLHPALLSFDFNSEESHNVKDLLLQCFLSINHIKREEGRRFLSFLFSWDASFIKMIHGTIKNQLQCLPKSLMTHIADIYFRAWKKASGDVLQMIENSCIQDFMHHGVHLPRNSPLHPKVREVLSYFHQQKLRQGVEEMLCRLYQPIIWRGLKARNSEVRSNAALLFVEAFPIRDPNLNHEDMDNEIQKQFEELFNLLEDPQPLVRSTGVLGVCKITAKYWEMIPPAILTDLLRKILGDLAADVSSADVRCSVFKCLPILLDNKLSHPLLEKMLPALKFCLHDNSEKVRVAFVDMLLKIKAVKAAKFWKICPMEQILARLEVDSRPVSRRIVNLLFNSFFPVNQQEEVWCERCVALIQMNPAAARKFYQYAYEHTAPTNIAKLMLTIRRCLNACIQRTVRNEDSEDEEDDEEIVRGDNEKENKSVLENVLSTDDSSSMASLLEIVVVLWRSIRKALEQNEEAKTYTISKFATVLPEYFKVFRDDRCTVPLIILASFMPPSAVPTFSCSVLSKLRHLDDGADEHKYSTLIDCLCRWGQVGHVLELATEWLSESYPEKRGRKDSNRQVRIQDTVESKPSLALDYIEYIVTHTMNRDCLLSLQTKKLNQLLKVLGLVKEVLFCYMKPSEAVTHNINQDTALRAFSLYCRLSIHLQHKFSSEGRTYLSLLEDTGGWIESQVLPTLESNGDLSEESCNMCHQILKAYLTVCKDVLMVGLADSEFQAQLLQITLSVIQTEKCHDCLPMLFSVLKEITELCLAHKMSDASVECDEMLDAIQRVFHKSLETVARGLRKQREEALPLLQAIQPSLGEFVHTVQCWHTASKVVHRGMLSTLLAAVVVEISHSLRKITDLSELTPPTSISDLPPLSKCIMTIIVKSPSAVSSFLDELTECITLEEVEGILSLSASLYVAVVCNKRKQIPPAVKNTASAIYRKLKNFSEVTMDDAGSIERAIYESSMRILDEMLHPS

Target ORF information:

RefSeq Version	XM_002935173.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis non-SMC condensin II complex subunit G2 (ncapg2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935173.4

ORF Insert Sequence:

ATGTCCAAGC GTGAGGCATT CTTGCAAGCC ACTGCCAAGG ATTCTGTAGA AGATTTCCTG 
AACTTCATAC AGCTTCATAA AGACGTGTCT GATCCTTTTG ATCTAAATGA ATTACTGCAA 
GAGTTGCCAA GGAAACAGAA GGAGGAATTA TGGGAGAAAC TAAAGACTCT TTTAACTGAT 
ACTTTGGTGG AAAACCCTGT GGAAGGGTGG CAAAATATTG ATGATGACAG TGATGATGAC 
ATGGAAGTAG AGAGTTCCTC AGATGTGAAA CAAACAATGT CTATAATCCA CGGGCTAACA 
ATAGCTGCTG CAGCTTCTGT GTGTGTCATA GATGAAGATG TGTGCTATGA AGCTCTCTTA 
GAATGTGCAG CAATCCTCAG TGGTATTGTT CATGCTTTGC CTAAATCCGA ATCCCACATT 
ATCCTTGCTA TCAGACATCT ATGTGAAGCC TGGTGGGAAA AAGGGCTCCA AGGAAAAGAA 
GAATTTGGGA AAACCGCCTT TCTTTTGCTT TTGGCAAAAT CTCTTGAAGT AAAGTGTGTG 
GTTGCTGATA TTGGGCGCTT GTGGCATCTG CATCCAGCTC TGCTCAGTTT TGATTTCAAC 
TCTGAAGAGA GTCACAACGT GAAGGATCTG CTGCTCCAGT GCTTCCTGAG CATTAACCAT 
ATTAAGAGAG AGGAGGGCAG AAGATTTCTT AGCTTTCTTT TCAGCTGGGA TGCCAGCTTC 
ATTAAAATGA TCCATGGGAC TATCAAAAAT CAGCTTCAGT GCTTACCAAA ATCGTTGATG 
ACCCACATCG CAGACATTTA CTTTAGGGCC TGGAAGAAAG CCTCGGGGGA TGTTTTACAG 
ATGATTGAGA ACAGCTGTAT TCAAGATTTT ATGCATCATG GAGTCCATCT ACCAAGGAAT 
TCTCCACTTC ACCCCAAAGT GCGAGAGGTG CTAAGTTACT TTCACCAACA GAAGCTACGT 
CAGGGTGTGG AAGAAATGCT TTGTAGACTG TATCAGCCCA TTATTTGGCG AGGCCTAAAG 
GCAAGGAATT CTGAAGTTCG GTCAAATGCT GCCCTGTTAT TTGTCGAAGC ATTTCCTATA 
AGGGACCCCA ATCTTAACCA TGAGGATATG GACAATGAAA TCCAGAAACA GTTTGAAGAA 
CTTTTTAATC TTTTGGAGGA TCCCCAGCCT CTTGTCCGTT CTACAGGGGT TTTAGGTGTC 
TGTAAGATAA CTGCTAAGTA CTGGGAAATG ATTCCTCCAG CCATTCTGAC TGATCTTCTG 
CGGAAAATAC TTGGCGATCT AGCTGCGGAT GTAAGCTCGG CCGATGTTCG TTGTTCTGTA 
TTTAAGTGTT TACCGATTCT GTTGGACAAC AAGTTAAGTC ATCCGCTGCT GGAGAAGATG 
CTGCCGGCAC TGAAATTCTG TCTCCATGAC AATTCTGAGA AAGTTCGAGT GGCTTTTGTT 
GATATGCTGC TGAAGATCAA AGCTGTCAAA GCGGCCAAGT TCTGGAAGAT CTGCCCTATG 
GAACAGATTT TGGCACGCCT TGAAGTGGAT TCCCGCCCTG TTTCTCGACG AATTGTTAAC 
CTTCTGTTTA ACTCTTTCTT TCCTGTCAAT CAACAAGAGG AGGTTTGGTG CGAGCGCTGT 
GTTGCTCTCA TCCAAATGAA CCCAGCCGCA GCACGAAAGT TTTATCAATA CGCATACGAG 
CATACTGCCC CCACCAATAT AGCTAAATTG ATGCTGACTA TACGCCGTTG TTTAAATGCC 
TGCATTCAGC GGACTGTTCG AAACGAAGAT AGTGAGGATG AGGAGGATGA CGAGGAAATT 
GTCAGAGGCG ATAATGAAAA GGAAAATAAA AGTGTTTTGG AGAATGTGTT ATCCACGGAT 
GATTCTTCCT CTATGGCGAG TTTGCTGGAG ATTGTTGTGG TTCTCTGGCG CAGTATCAGA 
AAAGCCCTTG AACAGAATGA AGAAGCCAAA ACCTACACCA TCAGCAAATT TGCTACAGTG 
CTTCCAGAAT ATTTCAAAGT ATTTAGGGAT GATCGCTGTA CTGTTCCGTT AATTATTCTG 
GCTTCCTTCA TGCCTCCATC TGCTGTCCCA ACTTTCAGCT GCTCTGTCTT ATCTAAGCTG 
AGACACTTGG ACGATGGCGC AGATGAGCAC AAATATTCCA CTCTTATTGA CTGTCTATGC 
CGCTGGGGGC AAGTGGGGCA TGTATTGGAG CTGGCAACCG AATGGCTCTC AGAGTCATAC 
CCAGAGAAAA GGGGGAGAAA AGATTCCAAT CGGCAAGTGC GTATCCAGGA CACAGTGGAA 
TCAAAGCCTT CGCTGGCCCT TGATTATATA GAATATATAG TTACCCACAC CATGAATCGA 
GATTGCCTTT TGTCCCTTCA AACAAAGAAA CTGAACCAAC TCCTGAAAGT GCTTGGTTTA 
GTAAAGGAGG TGCTGTTCTG CTACATGAAG CCATCCGAAG CAGTGACACA TAATATCAAC 
CAAGATACAG CATTAAGAGC CTTCAGCCTG TACTGCAGAC TCAGCATCCA CCTGCAACAT 
AAGTTTAGCT CTGAAGGAAG AACATATTTG TCTTTGCTGG AAGACACAGG AGGGTGGATC 
GAGAGCCAAG TCCTTCCAAC TCTAGAAAGT AATGGAGACC TCTCCGAGGA GTCATGTAAT 
ATGTGCCATC AGATCCTCAA GGCCTATCTG ACTGTTTGTA AAGACGTATT GATGGTTGGC 
CTCGCTGACT CGGAATTTCA AGCCCAGCTT CTTCAGATTA CTCTTTCAGT GATACAGACT 
GAGAAGTGCC ATGATTGTTT GCCAATGCTG TTCTCTGTGC TGAAGGAAAT CACAGAACTG 
TGTTTGGCTC ACAAGATGAG CGACGCCAGC GTGGAATGCG ACGAGATGCT CGATGCCATC 
CAGAGAGTAT TCCATAAGTC TTTGGAGACT GTCGCACGCG GGCTTAGAAA ACAGAGGGAA 
GAGGCACTAC CGCTTCTGCA AGCTATTCAG CCTTCTCTAG GAGAGTTTGT CCACACAGTT 
CAGTGCTGGC ACACCGCTTC CAAGGTGGTA CATCGAGGGA TGCTTTCCAC GCTGTTGGCT 
GCAGTGGTTG TTGAAATAAG CCACTCACTG CGCAAAATTA CAGATTTAAG TGAACTAACG 
CCACCCACAT CTATTTCTGA TCTGCCTCCG CTTTCAAAAT GTATAATGAC AATTATCGTT 
AAATCTCCAA GTGCTGTAAG TTCATTCTTG GATGAATTGA CAGAATGCAT CACATTAGAA 
GAAGTAGAAG GAATACTGAG CTTGTCCGCA TCTTTGTATG TTGCTGTTGT CTGCAATAAA 
AGGAAACAAA TACCCCCTGC AGTTAAGAAT ACTGCATCAG CAATTTATAG GAAACTGAAG 
AATTTCAGTG AGGTAACCAT GGACGATGCC GGAAGCATTG AAAGGGCTAT ATATGAATCA 
TCCATGAGGA TATTAGATGA AATGCTGCAT CCATCATAA