LOC100495290 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100495290
Entrez Gene ID	100495290
Full Name	collagen alpha-1(I) chain-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018090176.1	XP_017945665.1	collagen alpha-1(I) chain-like isoform X2
XM_018090175.2	XP_017945664.1	collagen alpha-1(I) chain isoform X1
XM_031891045.1	XP_031746905.1	collagen alpha-1(I) chain isoform X2
XM_018090175.1	XP_017945664.1	collagen alpha-1(I) chain-like isoform X1
XM_018090177.1	XP_017945666.1	collagen alpha-1(I) chain-like isoform X3
XM_002943596.4	XP_002943642.2	collagen alpha-1(I) chain-like isoform X4
XM_018090177.2	XP_017945666.1	collagen alpha-1(I) chain isoform X3
XM_002943596.5	XP_002943642.2	collagen alpha-1(I) chain isoform X4

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100495290	XP_002943642.2
Danio rerio (zebrafish)	LOC564099	XP_692548.3
Gallus gallus (chicken)	LOC101747382	XP_004945850.1

Gene Ontology

The following LOC100495290 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100495290 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa19428	XM_002943596.5 Latest version!	Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa41235	XM_018090175.1 Latest version!	Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa41236	XM_018090176.1 Latest version!	Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa41237	XM_018090177.1 Latest version!	Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa64301	XM_018090175.2 Latest version!	Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa64302	XM_031891045.1 Latest version!	Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa64303	XM_018090177.2 Latest version!	Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OXa19428	XM_002943596.4	Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100495290 ( XM_002943596.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002943642.2 Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa19428
Clone ID Related Accession (Same CDS sequence)	XM_002943596.4 , XM_002943596.5
Accession Version	XM_002943596.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(I) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002943596.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC CTGACATGTC CCTTAGTATC CGGAGGCTGT TGTTCCTACT CTGCTTGGCC 
CTCACCCCTA GCACTGCGGA CCCCCAGCCG ATTTCCCCAA CAAACACGAG TGGGAAGGTC 
CCCCAAGGAC GGGGCGTCGG GACACAACAT AGGGGCAGTC TGGACTCTCA GCGGCTCATC 
AACGAAGTGG ATCTGTTGCT TCTGGAAGAG GAAGGCTTCA TTCCCTCCTT CGTCGGGGGC 
GAGCAGGACG GCGCCAAGGC GGCCAAGAAG GAGGGACAAC ACAAAAAACT GGACCCCGCT 
GAAGAGAACA TCACTATAGA GAAGCAAGAA AAGGTCCCCG AGCGCCTTCC GACTGACAAA 
TCCAGTAGTG ACCTCCTAGA CTTGGCCAAA GACCCCAAAC CGATTCCATG GTCTACACCT 
GCTTCCACCA GTGCCCCCTT CCACTCCACG GCCGTTCCAG ATCTCTCTTT AGAAACAAAG 
AACAAGACAA GTATAGACAT GGCACCTACT CCGTCCCATG CCACCGATCC GCAGAAGGCT 
GCTGGGAACG CAAGCCGCGA GAGCAACGGC ATCCAGACAG GGAAACCCAA AAGACCCGTT 
GCCCGTGGGC CTCCGGGACC CCGTGGTAGT CCTGGCTGCC CTGGTCGTCC TGGATTTCCT 
GGACCAAAAG GAGACAAGGG CCAACCGGGA ATAAGGGGGC GTACAGGAGT GACTGGCAAC 
CCCGGTCCCA ACGGGCCCCC TGGACTACCC ACCGTAATGA TCTGGAGCAA CTCTAAAGAA 
GACTGGGCAG CTTTCTATCA AACGTCGTTC TATCAGCTGC TGCACGCAGG CTGGCCTAGA 
ATCCGTGGGT CGACCGGCTT CCCTGGGATG CCCGGAACGC CAGGAACTCC AGGTTCAATG 
GGACCCCCGG GGCGACCTGG AATCAAAGGA CAGCAAGGTT CCATGGGGAC CCTGGGGCCC 
AAGGGTATGC AAGGGATGCC GGGGACACCA GGAAGGGACG GATCTCCGGG ATTAGACGGG 
AAGCCGGGAT CTCCGGGGAC GACAGGAGCC AAAGGCCCTC AAGGATTTAA AGGGGACCAA 
GGACCTCTCG GCGAGAAAGG AGAAGAAGGA TTTCAAGGAT CTCCAGGGCC AAGTGGTGAG 
GAAGGAGTCA AAGGAGCCAA GGGGGTGAAG GGAGAGGCCG GTCCCGTGGG TCCTTCGGGG 
GTTCAGGGCC CAATGGGTGC TAAAGGTTCT TTGGGATTTC CAGGCCCCCC AGGACCAATA 
GGAGATATGG GAATTCCTGG AACTCCTGGT CCACCGGGGC CTGAGGGAGA ACAAGGACAG 
CCAGGAACCC CAGGAACCCA GGGCTTGAAT GGGTCCCAGG GTGATCTGGG TCCTCCGGGA 
ATAATTGGTC ATCGGGGACC ACAGGGTCCT CCTGGAATAG AAGGTATAAG AGGACCTCCT 
GGGCCCAGAG GACCACAGGG ATTGATGGGA GCCGATGGAC CCCAAGGTCC ACAAGGAAGC 
ACAGGGGACC CTGGGCCAAT GGGAGTAAGA GGAGAACGGG GACTTGAAGG ACCCATGGGA 
TTGATTGGAT GGCCAGGACC AGATGGACCT CCTGGTGATA AGGGTCGCCC TGCTGCAGAT 
GCCGAAAAAG GAGATCCTGG ACCAAAGGGG GAAAAAGGTT GTCCAGGATC TCCTGGCATC 
AAAGGTCCTT CTGGAGAGCA AGGCCATGTT GGTTTTGTGG GTTTCCCAGG ACCTAAAGGT 
CAGATGGGCC CCATCGGAGC TCCAGGGGAT GAAGGCCAGG TTGGAGCTTC AGGTCTCAAC 
GGCAGCGACG GACCAAAGGG AAACCGGGGA CCTGATGGGC CAAAGGGGGT AACAGGACCA 
AGAGGAAATC AGGGTCGTAT GGGCCAGAGA GGACTTGTAG GAATGCCAGG AGCATCAGGT 
CAGCGAGGAG CCGGGGGCCC CGAGGGAGCA GAAGGACTTC CTGGTACACA GGGACCCAGT 
GGCGCAACAG GGAATCCTGG GAGTAGGGGG CTTACGGGTC CTGAAGGGTC TCAAGGGGAC 
GCTGGAAGAG AAGGACCTCC AGGCCAAATG GGGCCTCCTG GGAGAGAAGG GATTGATGGA 
GAACCAGGAG ATTCTGGCGA AAGGGGATTT ACAGGGAAAC CAGGCCCAGA AGGGCCACAG 
GGGCCAGTAG GCATGTATGG GTTCCATGGC CCTGCTGGAG AGAGAGGTCG GACGGGATAC 
CCCGGGGAGA AGGGGGAATT GGGACCTCCT GGGATCCAAG GGCCACCAGG AAAAGTGGGA 
CTAAAGGGTC CTCCTGGGCC ACCAGGGGAA AGAGGCCCAC CAGGACCCCA GGGTCGACTA 
GGAGAACCAG GCATCCAGGG TTTGCCCGGC CTTCAGGGCC CAGCTGGTAA CCCGGGGCCA 
GATGGGGAGC CTGGAAAGGG TGGACGTCCA GGTGAAACCG GGCCTGCTGG GAGTATAGGA 
GACCCAGGAT CTCCAGGATA CCAGGGTCGA GACGGAGCAG CAGGTGCTGA AGGACCCCCA 
GGAGAGAAAG GAGAAAAGGG AGAAATTGGG CCTGTAGGAG ACCAAGGGGT GAGCGGTTTG 
GACGGGGAGC AGGGAGAACC TGGGTCTACT GGACATGAAG GATCAGTTGG CCATAAAGGA 
GATCAGGGGG ACCCTGGTCC TCCAGGGAAT CCTGGTCTAA ACGGGCCTGT AGGAGACCTA 
GGGGCAAGAG GACTGCAGGG CCTGGTGGGA CCCCCAGGGG AACCAGGGAA GGAGGGAGAT 
GTGGGGGAAA TTGGAGATGT TGGAGACCAC GGTCCTCAAG GAGAAAAGGG AGACAAAGGG 
CCACTGGGTA GTACCGGGAA CCCAGGACCT GGCGGCATTA TAGGAGAACG AGGCAATCGA 
GGTGAAAAGG GGGACAAAGG CCTGAGTGGA CCTCTGGGAC CCCCGGGGTT GATTGGATCT 
ACAGGACCTT CTGGTCTACA GGGAGAAATG GGAAATGTTG GCAAACAAGG GTCCCCAGGT 
CTTGATGGAC CTCAAGGTTT GAAGGGGGAA ACTGGTTCCA CTGGAGAGAC TGGTCAACTC 
GGTCCCCCTG GGCCAATGGG CCCACCTGGA AAGAAAGGGG AGAAGGGGGA GCGAGGACAG 
ACTGGGAGAC CAGGAACAGA GGGGCCACGA GGCATTAACG GCAACCAGGG GCCACAAGGT 
TTTATTGGCC AAGAGGGACT CAAGGGGGAA GATGGGAGCC CGGGAAATAC AGGAATCCTG 
GGGGTTACGG GGCCACGTGG GGAGTCTGGA CCCATAGGGG CACCAGGAAG AGAAGGGAAG 
GAAGGAGAAA AGGGGCAACC AGGAGAACAT GGAGTTCCAG GGAAACCGGG GCCCCCTGGA 
AACCCAGGAC AAGAAGGGAA TGTGGGGCAG AGGGGAGAAA AGGGTGACAG TGGCATTGAT 
GGCCGCCCTG GCAGAGCAGG GAATCCTGGG AGAAAGGGCA GAAGAGGGAA AGCAGGTCCA 
AGGCCACCAA GAGGACCTGC AGGTCTCAAG GGTCTACAAG GAGATAAGGG TTCGGAAGGA 
CCAGAAGGAC CCAGAGGACC ATATGGCATT CCTGGACTCA GAGGAACGAA AGGTGAACCG 
GGTCGAAGAG GTATAAAGGG TGAGCTGGGC TTGGATGGAC CAATAGGACT GCAAGGAGAA 
GAAGGGCTGA AGGGACCACA AGGACCGACC GGGGAGCGGG GACCTTTCGG GATCAAAGGG 
GAACAGGGAA TTGAGGGGCC ACCTGGCTCT CAAGGATTAC CTGGACCCCA AGGTGCACCA 
GGACCACCAG GGATAGAGGG ACATAAAGGA TTCTCTGGAT CTGAAGGGCC CAGAGGGCCA 
CCTGGACCAC CGGGGCTTCC TGGTGCTCCT GGACTCCCAG GACCTTCTCT AAACTTAACG 
GCCCAAAGTT TAAAGCGGAC CTTCTTCCAT GCCGGGGCCT TGAGCGTAGA GGGACCTTGG 
GATCTTCTGC ATCGTTTGGA AGAGGACTTG AAACACTTGC GCTCTCCGCC CAATGGTTCC 
AAAGAAAACC CAGCCAGCAC CTGTAAGGAA CTTCAGCTGG TGCAACCTCA TCTGTCGGAT 
GGTCATTACT ATGTAGATCC CAACCAGGGG AGTCCACAGG ATTCTTTCCT TGTGTTCTGT 
AACTTCACTG CAGGCGGTGA GACGTGTCTG CTCCCTGAGG AGAGCCAAAT ACCTGTCAGA 
CCTTGGCTTC AGGACTATCA GGCTTCTGAG GGTTTCCAGT GGTTCAGCAC TCTTCCTGGG 
GGCTTTATGT TCAGGTACAG TGAAACAGGA GTCGTCCAGC TGCGGTTCCT GAGGCTACAG 
AGCAGCGCCG TACATCAGGG GGTGAGCTAC GTGTGCCAAG GTCAGCCCGG CGTCGCTGCG 
AAAGACAAGA ATGTGAAGTT TCTGACCGAT ACCCGGGATC AGAGCTTTCT AGTGACTCTG 
CAGGGCTGTG AGTGGGAAGA GACTTCAAAC AGAACAGGAA CTCTCCTCCA CTTTGCCCCC 
TCAGACCTCA GCCTCCTTCC TCTCAGAGAC TTGGCCATGT TACACGATGG GGACATTGGG 
CACCAGTTTG GTTTCTTGGT GGGTCCTACC TGCTACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002943642.2
CDS	136..4737
Translation	MGSPDMSLSIRRLLFLLCLALTPSTADPQPISPTNTSGKVPQGRGVGTQHRGSLDSQRLINEVDLLLLEEEGFIPSFVGGEQDGAKAAKKEGQHKKLDPAEENITIEKQEKVPERLPTDKSSSDLLDLAKDPKPIPWSTPASTSAPFHSTAVPDLSLETKNKTSIDMAPTPSHATDPQKAAGNASRESNGIQTGKPKRPVARGPPGPRGSPGCPGRPGFPGPKGDKGQPGIRGRTGVTGNPGPNGPPGLPTVMIWSNSKEDWAAFYQTSFYQLLHAGWPRIRGSTGFPGMPGTPGTPGSMGPPGRPGIKGQQGSMGTLGPKGMQGMPGTPGRDGSPGLDGKPGSPGTTGAKGPQGFKGDQGPLGEKGEEGFQGSPGPSGEEGVKGAKGVKGEAGPVGPSGVQGPMGAKGSLGFPGPPGPIGDMGIPGTPGPPGPEGEQGQPGTPGTQGLNGSQGDLGPPGIIGHRGPQGPPGIEGIRGPPGPRGPQGLMGADGPQGPQGSTGDPGPMGVRGERGLEGPMGLIGWPGPDGPPGDKGRPAADAEKGDPGPKGEKGCPGSPGIKGPSGEQGHVGFVGFPGPKGQMGPIGAPGDEGQVGASGLNGSDGPKGNRGPDGPKGVTGPRGNQGRMGQRGLVGMPGASGQRGAGGPEGAEGLPGTQGPSGATGNPGSRGLTGPEGSQGDAGREGPPGQMGPPGREGIDGEPGDSGERGFTGKPGPEGPQGPVGMYGFHGPAGERGRTGYPGEKGELGPPGIQGPPGKVGLKGPPGPPGERGPPGPQGRLGEPGIQGLPGLQGPAGNPGPDGEPGKGGRPGETGPAGSIGDPGSPGYQGRDGAAGAEGPPGEKGEKGEIGPVGDQGVSGLDGEQGEPGSTGHEGSVGHKGDQGDPGPPGNPGLNGPVGDLGARGLQGLVGPPGEPGKEGDVGEIGDVGDHGPQGEKGDKGPLGSTGNPGPGGIIGERGNRGEKGDKGLSGPLGPPGLIGSTGPSGLQGEMGNVGKQGSPGLDGPQGLKGETGSTGETGQLGPPGPMGPPGKKGEKGERGQTGRPGTEGPRGINGNQGPQGFIGQEGLKGEDGSPGNTGILGVTGPRGESGPIGAPGREGKEGEKGQPGEHGVPGKPGPPGNPGQEGNVGQRGEKGDSGIDGRPGRAGNPGRKGRRGKAGPRPPRGPAGLKGLQGDKGSEGPEGPRGPYGIPGLRGTKGEPGRRGIKGELGLDGPIGLQGEEGLKGPQGPTGERGPFGIKGEQGIEGPPGSQGLPGPQGAPGPPGIEGHKGFSGSEGPRGPPGPPGLPGAPGLPGPSLNLTAQSLKRTFFHAGALSVEGPWDLLHRLEEDLKHLRSPPNGSKENPASTCKELQLVQPHLSDGHYYVDPNQGSPQDSFLVFCNFTAGGETCLLPEESQIPVRPWLQDYQASEGFQWFSTLPGGFMFRYSETGVVQLRFLRLQSSAVHQGVSYVCQGQPGVAAKDKNVKFLTDTRDQSFLVTLQGCEWEETSNRTGTLLHFAPSDLSLLPLRDLAMLHDGDIGHQFGFLVGPTCYS

Target ORF information:

RefSeq Version	XM_002943596.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002943596.5

ORF Insert Sequence:

ATGGGGAGCC CTGACATGTC CCTTAGTATC CGGAGGCTGT TGTTCCTACT CTGCTTGGCC 
CTCACCCCTA GCACTGCGGA CCCCCAGCCG ATTTCCCCAA CAAACACGAG TGGGAAGGTC 
CCCCAAGGAC GGGGCGTCGG GACACAACAT AGGGGCAGTC TGGACTCTCA GCGGCTCATC 
AACGAAGTGG ATCTGTTGCT TCTGGAAGAG GAAGGCTTCA TTCCCTCCTT CGTCGGGGGC 
GAGCAGGACG GCGCCAAGGC GGCCAAGAAG GAGGGACAAC ACAAAAAACT GGACCCCGCT 
GAAGAGAACA TCACTATAGA GAAGCAAGAA AAGGTCCCCG AGCGCCTTCC GACTGACAAA 
TCCAGTAGTG ACCTCCTAGA CTTGGCCAAA GACCCCAAAC CGATTCCATG GTCTACACCT 
GCTTCCACCA GTGCCCCCTT CCACTCCACG GCCGTTCCAG ATCTCTCTTT AGAAACAAAG 
AACAAGACAA GTATAGACAT GGCACCTACT CCGTCCCATG CCACCGATCC GCAGAAGGCT 
GCTGGGAACG CAAGCCGCGA GAGCAACGGC ATCCAGACAG GGAAACCCAA AAGACCCGTT 
GCCCGTGGGC CTCCGGGACC CCGTGGTAGT CCTGGCTGCC CTGGTCGTCC TGGATTTCCT 
GGACCAAAAG GAGACAAGGG CCAACCGGGA ATAAGGGGGC GTACAGGAGT GACTGGCAAC 
CCCGGTCCCA ACGGGCCCCC TGGACTACCC ACCGTAATGA TCTGGAGCAA CTCTAAAGAA 
GACTGGGCAG CTTTCTATCA AACGTCGTTC TATCAGCTGC TGCACGCAGG CTGGCCTAGA 
ATCCGTGGGT CGACCGGCTT CCCTGGGATG CCCGGAACGC CAGGAACTCC AGGTTCAATG 
GGACCCCCGG GGCGACCTGG AATCAAAGGA CAGCAAGGTT CCATGGGGAC CCTGGGGCCC 
AAGGGTATGC AAGGGATGCC GGGGACACCA GGAAGGGACG GATCTCCGGG ATTAGACGGG 
AAGCCGGGAT CTCCGGGGAC GACAGGAGCC AAAGGCCCTC AAGGATTTAA AGGGGACCAA 
GGACCTCTCG GCGAGAAAGG AGAAGAAGGA TTTCAAGGAT CTCCAGGGCC AAGTGGTGAG 
GAAGGAGTCA AAGGAGCCAA GGGGGTGAAG GGAGAGGCCG GTCCCGTGGG TCCTTCGGGG 
GTTCAGGGCC CAATGGGTGC TAAAGGTTCT TTGGGATTTC CAGGCCCCCC AGGACCAATA 
GGAGATATGG GAATTCCTGG AACTCCTGGT CCACCGGGGC CTGAGGGAGA ACAAGGACAG 
CCAGGAACCC CAGGAACCCA GGGCTTGAAT GGGTCCCAGG GTGATCTGGG TCCTCCGGGA 
ATAATTGGTC ATCGGGGACC ACAGGGTCCT CCTGGAATAG AAGGTATAAG AGGACCTCCT 
GGGCCCAGAG GACCACAGGG ATTGATGGGA GCCGATGGAC CCCAAGGTCC ACAAGGAAGC 
ACAGGGGACC CTGGGCCAAT GGGAGTAAGA GGAGAACGGG GACTTGAAGG ACCCATGGGA 
TTGATTGGAT GGCCAGGACC AGATGGACCT CCTGGTGATA AGGGTCGCCC TGCTGCAGAT 
GCCGAAAAAG GAGATCCTGG ACCAAAGGGG GAAAAAGGTT GTCCAGGATC TCCTGGCATC 
AAAGGTCCTT CTGGAGAGCA AGGCCATGTT GGTTTTGTGG GTTTCCCAGG ACCTAAAGGT 
CAGATGGGCC CCATCGGAGC TCCAGGGGAT GAAGGCCAGG TTGGAGCTTC AGGTCTCAAC 
GGCAGCGACG GACCAAAGGG AAACCGGGGA CCTGATGGGC CAAAGGGGGT AACAGGACCA 
AGAGGAAATC AGGGTCGTAT GGGCCAGAGA GGACTTGTAG GAATGCCAGG AGCATCAGGT 
CAGCGAGGAG CCGGGGGCCC CGAGGGAGCA GAAGGACTTC CTGGTACACA GGGACCCAGT 
GGCGCAACAG GGAATCCTGG GAGTAGGGGG CTTACGGGTC CTGAAGGGTC TCAAGGGGAC 
GCTGGAAGAG AAGGACCTCC AGGCCAAATG GGGCCTCCTG GGAGAGAAGG GATTGATGGA 
GAACCAGGAG ATTCTGGCGA AAGGGGATTT ACAGGGAAAC CAGGCCCAGA AGGGCCACAG 
GGGCCAGTAG GCATGTATGG GTTCCATGGC CCTGCTGGAG AGAGAGGTCG GACGGGATAC 
CCCGGGGAGA AGGGGGAATT GGGACCTCCT GGGATCCAAG GGCCACCAGG AAAAGTGGGA 
CTAAAGGGTC CTCCTGGGCC ACCAGGGGAA AGAGGCCCAC CAGGACCCCA GGGTCGACTA 
GGAGAACCAG GCATCCAGGG TTTGCCCGGC CTTCAGGGCC CAGCTGGTAA CCCGGGGCCA 
GATGGGGAGC CTGGAAAGGG TGGACGTCCA GGTGAAACCG GGCCTGCTGG GAGTATAGGA 
GACCCAGGAT CTCCAGGATA CCAGGGTCGA GACGGAGCAG CAGGTGCTGA AGGACCCCCA 
GGAGAGAAAG GAGAAAAGGG AGAAATTGGG CCTGTAGGAG ACCAAGGGGT GAGCGGTTTG 
GACGGGGAGC AGGGAGAACC TGGGTCTACT GGACATGAAG GATCAGTTGG CCATAAAGGA 
GATCAGGGGG ACCCTGGTCC TCCAGGGAAT CCTGGTCTAA ACGGGCCTGT AGGAGACCTA 
GGGGCAAGAG GACTGCAGGG CCTGGTGGGA CCCCCAGGGG AACCAGGGAA GGAGGGAGAT 
GTGGGGGAAA TTGGAGATGT TGGAGACCAC GGTCCTCAAG GAGAAAAGGG AGACAAAGGG 
CCACTGGGTA GTACCGGGAA CCCAGGACCT GGCGGCATTA TAGGAGAACG AGGCAATCGA 
GGTGAAAAGG GGGACAAAGG CCTGAGTGGA CCTCTGGGAC CCCCGGGGTT GATTGGATCT 
ACAGGACCTT CTGGTCTACA GGGAGAAATG GGAAATGTTG GCAAACAAGG GTCCCCAGGT 
CTTGATGGAC CTCAAGGTTT GAAGGGGGAA ACTGGTTCCA CTGGAGAGAC TGGTCAACTC 
GGTCCCCCTG GGCCAATGGG CCCACCTGGA AAGAAAGGGG AGAAGGGGGA GCGAGGACAG 
ACTGGGAGAC CAGGAACAGA GGGGCCACGA GGCATTAACG GCAACCAGGG GCCACAAGGT 
TTTATTGGCC AAGAGGGACT CAAGGGGGAA GATGGGAGCC CGGGAAATAC AGGAATCCTG 
GGGGTTACGG GGCCACGTGG GGAGTCTGGA CCCATAGGGG CACCAGGAAG AGAAGGGAAG 
GAAGGAGAAA AGGGGCAACC AGGAGAACAT GGAGTTCCAG GGAAACCGGG GCCCCCTGGA 
AACCCAGGAC AAGAAGGGAA TGTGGGGCAG AGGGGAGAAA AGGGTGACAG TGGCATTGAT 
GGCCGCCCTG GCAGAGCAGG GAATCCTGGG AGAAAGGGCA GAAGAGGGAA AGCAGGTCCA 
AGGCCACCAA GAGGACCTGC AGGTCTCAAG GGTCTACAAG GAGATAAGGG TTCGGAAGGA 
CCAGAAGGAC CCAGAGGACC ATATGGCATT CCTGGACTCA GAGGAACGAA AGGTGAACCG 
GGTCGAAGAG GTATAAAGGG TGAGCTGGGC TTGGATGGAC CAATAGGACT GCAAGGAGAA 
GAAGGGCTGA AGGGACCACA AGGACCGACC GGGGAGCGGG GACCTTTCGG GATCAAAGGG 
GAACAGGGAA TTGAGGGGCC ACCTGGCTCT CAAGGATTAC CTGGACCCCA AGGTGCACCA 
GGACCACCAG GGATAGAGGG ACATAAAGGA TTCTCTGGAT CTGAAGGGCC CAGAGGGCCA 
CCTGGACCAC CGGGGCTTCC TGGTGCTCCT GGACTCCCAG GACCTTCTCT AAACTTAACG 
GCCCAAAGTT TAAAGCGGAC CTTCTTCCAT GCCGGGGCCT TGAGCGTAGA GGGACCTTGG 
GATCTTCTGC ATCGTTTGGA AGAGGACTTG AAACACTTGC GCTCTCCGCC CAATGGTTCC 
AAAGAAAACC CAGCCAGCAC CTGTAAGGAA CTTCAGCTGG TGCAACCTCA TCTGTCGGAT 
GGTCATTACT ATGTAGATCC CAACCAGGGG AGTCCACAGG ATTCTTTCCT TGTGTTCTGT 
AACTTCACTG CAGGCGGTGA GACGTGTCTG CTCCCTGAGG AGAGCCAAAT ACCTGTCAGA 
CCTTGGCTTC AGGACTATCA GGCTTCTGAG GGTTTCCAGT GGTTCAGCAC TCTTCCTGGG 
GGCTTTATGT TCAGGTACAG TGAAACAGGA GTCGTCCAGC TGCGGTTCCT GAGGCTACAG 
AGCAGCGCCG TACATCAGGG GGTGAGCTAC GTGTGCCAAG GTCAGCCCGG CGTCGCTGCG 
AAAGACAAGA ATGTGAAGTT TCTGACCGAT ACCCGGGATC AGAGCTTTCT AGTGACTCTG 
CAGGGCTGTG AGTGGGAAGA GACTTCAAAC AGAACAGGAA CTCTCCTCCA CTTTGCCCCC 
TCAGACCTCA GCCTCCTTCC TCTCAGAGAC TTGGCCATGT TACACGATGG GGACATTGGG 
CACCAGTTTG GTTTCTTGGT GGGTCCTACC TGCTACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495290 ( XM_018090175.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945664.1 Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa41235
Clone ID Related Accession (Same CDS sequence)	XM_018090175.1
Accession Version	XM_018090175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(I) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683627.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCGAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGTT GGATGTGGGC GGAGACTGGA CTACAGATCA TGGCAAAAAC 
TACACCCGTC AATCAACCAT ACTTGAGATT TCCCCAACAA ACACGAGTGG GAAGGTCCCC 
CAAGGACGGG GCGTCGGGAC ACAACATAGG GGCAGTCTGG ACTCTCAGCG GCTCATCAAC 
GAAGTGGATC TGTTGCTTCT GGAAGAGGAA GGCTTCATTC CCTCCTTCGT CGGGGGCGAG 
CAGGACGGCG CCAAGGCGGC CAAGAAGGAG GGACAACACA AAAAACTGGA CCCCGCTGAA 
GAGAACATCA CTATAGAGAA GCAAGAAAAG GTCCCCGAGC GCCTTCCGAC TGACAAATCC 
AGTAGTGACC TCCTAGACTT GGCCAAAGAC CCCAAACCGA TTCCATGGTC TACACCTGCT 
TCCACCAGTG CCCCCTTCCA CTCCACGGCC GTTCCAGATC TCTCTTTAGA AACAAAGAAC 
AAGACAAGTA TAGACATGGC ACCTACTCCG TCCCATGCCA CCGATCCGCA GAAGGCTGCT 
GGGAACGCAA GCCGCGAGAG CAACGGCATC CAGACAGGGA AACCCAAAAG ACCCGTTGCC 
CGTGGGCCTC CGGGACCCCG TGGTAGTCCT GGCTGCCCTG GTCGTCCTGG ATTTCCTGGA 
CCAAAAGGAG ACAAGGGCCA ACCGGGAATA AGGGGGCGTA CAGGAGTGAC TGGCAACCCC 
GGTCCCAACG GGCCCCCTGG ACTACCCACC GTAATGATCT GGAGCAACTC TAAAGAAGAC 
TGGGCAGCTT TCTATCAAAC GTCGTTCTAT CAGCTGCTGC ACGCAGGCTG GCCTAGAATC 
CGTGGGTCGA CCGGCTTCCC TGGGATGCCC GGAACGCCAG GAACTCCAGG TTCAATGGGA 
CCCCCGGGGC GACCTGGAAT CAAAGGACAG CAAGGTTCCA TGGGGACCCT GGGGCCCAAG 
GGTATGCAAG GGATGCCGGG GACACCAGGA AGGGACGGAT CTCCGGGATT AGACGGGAAG 
CCGGGATCTC CGGGGACGAC AGGAGCCAAA GGCCCTCAAG GATTTAAAGG GGACCAAGGA 
CCTCTCGGCG AGAAAGGAGA AGAAGGATTT CAAGGATCTC CAGGGCCAAG TGGTGAGGAA 
GGAGTCAAAG GAGCCAAGGG GGTGAAGGGA GAGGCCGGTC CCGTGGGTCC TTCGGGGGTT 
CAGGGCCCAA TGGGTGCTAA AGGTTCTTTG GGATTTCCAG GCCCCCCAGG ACCAATAGGA 
GATATGGGAA TTCCTGGAAC TCCTGGTCCA CCGGGGCCTG AGGGAGAACA AGGACAGCCA 
GGAACCCCAG GAACCCAGGG CTTGAATGGG TCCCAGGGTG ATCTGGGTCC TCCGGGAATA 
ATTGGTCATC GGGGACCACA GGGTCCTCCT GGAATAGAAG GTATAAGAGG ACCTCCTGGG 
CCCAGAGGAC CACAGGGATT GATGGGAGCC GATGGACCCC AAGGTCCACA AGGAAGCACA 
GGGGACCCTG GGCCAATGGG AGTAAGAGGA GAACGGGGAC TTGAAGGACC CATGGGATTG 
ATTGGATGGC CAGGACCAGA TGGACCTCCT GGTGATAAGG GTCGCCCTGC TGCAGATGCC 
GAAAAAGGAG ATCCTGGACC AAAGGGGGAA AAAGGTTGTC CAGGATCTCC TGGCATCAAA 
GGTCCTTCTG GAGAGCAAGG CCATGTTGGT TTTGTGGGTT TCCCAGGACC TAAAGGTCAG 
ATGGGCCCCA TCGGAGCTCC AGGGGATGAA GGCCAGGTTG GAGCTTCAGG TCTCAACGGC 
AGCGACGGAC CAAAGGGAAA CCGGGGACCT GATGGGCCAA AGGGGGTAAC AGGACCAAGA 
GGAAATCAGG GTCGTATGGG CCAGAGAGGA CTTGTAGGAA TGCCAGGAGC ATCAGGTCAG 
CGAGGAGCCG GGGGCCCCGA GGGAGCAGAA GGACTTCCTG GTACACAGGG ACCCAGTGGC 
GCAACAGGGA ATCCTGGGAG TAGGGGGCTT ACGGGTCCTG AAGGGTCTCA AGGGGACGCT 
GGAAGAGAAG GACCTCCAGG CCAAATGGGG CCTCCTGGGA GAGAAGGGAT TGATGGAGAA 
CCAGGAGATT CTGGCGAAAG GGGATTTACA GGGAAACCAG GCCCAGAAGG GCCACAGGGG 
CCAGTAGGCA TGTATGGGTT CCATGGCCCT GCTGGAGAGA GAGGTCGGAC GGGATACCCC 
GGGGAGAAGG GGGAATTGGG ACCTCCTGGG ATCCAAGGGC CACCAGGAAA AGTGGGACTA 
AAGGGTCCTC CTGGGCCACC AGGGGAAAGA GGCCCACCAG GACCCCAGGG TCGACTAGGA 
GAACCAGGCA TCCAGGGTTT GCCCGGCCTT CAGGGCCCAG CTGGTAACCC GGGGCCAGAT 
GGGGAGCCTG GAAAGGGTGG ACGTCCAGGT GAAACCGGGC CTGCTGGGAG TATAGGAGAC 
CCAGGATCTC CAGGATACCA GGGTCGAGAC GGAGCAGCAG GTGCTGAAGG ACCCCCAGGA 
GAGAAAGGAG AAAAGGGAGA AATTGGGCCT GTAGGAGACC AAGGGGTGAG CGGTTTGGAC 
GGGGAGCAGG GAGAACCTGG GTCTACTGGA CATGAAGGAT CAGTTGGCCA TAAAGGAGAT 
CAGGGGGACC CTGGTCCTCC AGGGAATCCT GGTCTAAACG GGCCTGTAGG AGACCTAGGG 
GCAAGAGGAC TGCAGGGCCT GGTGGGACCC CCAGGGGAAC CAGGGAAGGA GGGAGATGTG 
GGGGAAATTG GAGATGTTGG AGACCACGGT CCTCAAGGAG AAAAGGGAGA CAAAGGGCCA 
CTGGGTAGTA CCGGGAACCC AGGACCTGGC GGCATTATAG GAGAACGAGG CAATCGAGGT 
GAAAAGGGGG ACAAAGGCCT GAGTGGACCT CTGGGACCCC CGGGGTTGAT TGGATCTACA 
GGACCTTCTG GTCTACAGGG AGAAATGGGA AATGTTGGCA AACAAGGGTC CCCAGGTCTT 
GATGGACCTC AAGGTTTGAA GGGGGAAACT GGTTCCACTG GAGAGACTGG TCAACTCGGT 
CCCCCTGGGC CAATGGGCCC ACCTGGAAAG AAAGGGGAGA AGGGGGAGCG AGGACAGACT 
GGGAGACCAG GAACAGAGGG GCCACGAGGC ATTAACGGCA ACCAGGGGCC ACAAGGTTTT 
ATTGGCCAAG AGGGACTCAA GGGGGAAGAT GGGAGCCCGG GAAATACAGG AATCCTGGGG 
GTTACGGGGC CACGTGGGGA GTCTGGACCC ATAGGGGCAC CAGGAAGAGA AGGGAAGGAA 
GGAGAAAAGG GGCAACCAGG AGAACATGGA GTTCCAGGGA AACCGGGGCC CCCTGGAAAC 
CCAGGACAAG AAGGGAATGT GGGGCAGAGG GGAGAAAAGG GTGACAGTGG CATTGATGGC 
CGCCCTGGCA GAGCAGGGAA TCCTGGGAGA AAGGGCAGAA GAGGGAAAGC AGGTCCAAGG 
CCACCAAGAG GACCTGCAGG TCTCAAGGGT CTACAAGGAG ATAAGGGTTC GGAAGGACCA 
GAAGGACCCA GAGGACCATA TGGCATTCCT GGACTCAGAG GAACGAAAGG TGAACCGGGT 
CGAAGAGGTA TAAAGGGTGA GCTGGGCTTG GATGGACCAA TAGGACTGCA AGGAGAAGAA 
GGGCTGAAGG GACCACAAGG ACCGACCGGG GAGCGGGGAC CTTTCGGGAT CAAAGGGGAA 
CAGGGAATTG AGGGGCCACC TGGCTCTCAA GGATTACCTG GACCCCAAGG TGCACCAGGA 
CCACCAGGGA TAGAGGGACA TAAAGGATTC TCTGGATCTG AAGGGCCCAG AGGGCCACCT 
GGACCACCGG GGCTTCCTGG TGCTCCTGGA CTCCCAGGAC CTTCTCTAAA CTTAACGGCC 
CAAAGTTTAA AGCGGACCTT CTTCCATGCC GGGGCCTTGA GCGTAGAGGG ACCTTGGGAT 
CTTCTGCATC GTTTGGAAGA GGACTTGAAA CACTTGCGCT CTCCGCCCAA TGGTTCCAAA 
GAAAACCCAG CCAGCACCTG TAAGGAACTT CAGCTGGTGC AACCTCATCT GTCGGATGGT 
CATTACTATG TAGATCCCAA CCAGGGGAGT CCACAGGATT CTTTCCTTGT GTTCTGTAAC 
TTCACTGCAG GCGGTGAGAC GTGTCTGCTC CCTGAGGAGA GCCAAATACC TGTCAGACCT 
TGGCTTCAGG ACTATCAGGC TTCTGAGGGT TTCCAGTGGT TCAGCACTCT TCCTGGGGGC 
TTTATGTTCA GGTACAGTGA AACAGGAGTC GTCCAGCTGC GGTTCCTGAG GCTACAGAGC 
AGCGCCGTAC ATCAGGGGGT GAGCTACGTG TGCCAAGGTC AGCCCGGCGT CGCTGCGAAA 
GACAAGAATG TGAAGTTTCT GACCGATACC CGGGATCAGA GCTTTCTAGT GACTCTGCAG 
GGCTGTGAGT GGGAAGAGAC TTCAAACAGA ACAGGAACTC TCCTCCACTT TGCCCCCTCA 
GACCTCAGCC TCCTTCCTCT CAGAGACTTG GCCATGTTAC ACGATGGGGA CATTGGGCAC 
CAGTTTGGTT TCTTGGTGGG TCCTACCTGC TACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945664.1
CDS	58..5436
Translation	MGRGGLSAAAIRTLGHRWALLLTLLLYRGAAGEQGDTPEAGSGQMAEEVDVLESLTLHSAELVNLTLTSEDSCLALELGQYSTLSISTMTAFGDSFADELTVLMMLRSSLREDTGLLSILSLHGLILFQIRINPKAFIFISSRRGHYEFSVPTLTDGQWHRVALSISSKGVALYVDCKLVEMLSWTNYFGLGVSTDGILMLGGLIEPYEVPFKGSLQQLVFVMGEPGAAKLYCRSYNQTCLSTFSEEHFFNLSQGDLRRREARVTKLDVGGDWTTDHGKNYTRQSTILEISPTNTSGKVPQGRGVGTQHRGSLDSQRLINEVDLLLLEEEGFIPSFVGGEQDGAKAAKKEGQHKKLDPAEENITIEKQEKVPERLPTDKSSSDLLDLAKDPKPIPWSTPASTSAPFHSTAVPDLSLETKNKTSIDMAPTPSHATDPQKAAGNASRESNGIQTGKPKRPVARGPPGPRGSPGCPGRPGFPGPKGDKGQPGIRGRTGVTGNPGPNGPPGLPTVMIWSNSKEDWAAFYQTSFYQLLHAGWPRIRGSTGFPGMPGTPGTPGSMGPPGRPGIKGQQGSMGTLGPKGMQGMPGTPGRDGSPGLDGKPGSPGTTGAKGPQGFKGDQGPLGEKGEEGFQGSPGPSGEEGVKGAKGVKGEAGPVGPSGVQGPMGAKGSLGFPGPPGPIGDMGIPGTPGPPGPEGEQGQPGTPGTQGLNGSQGDLGPPGIIGHRGPQGPPGIEGIRGPPGPRGPQGLMGADGPQGPQGSTGDPGPMGVRGERGLEGPMGLIGWPGPDGPPGDKGRPAADAEKGDPGPKGEKGCPGSPGIKGPSGEQGHVGFVGFPGPKGQMGPIGAPGDEGQVGASGLNGSDGPKGNRGPDGPKGVTGPRGNQGRMGQRGLVGMPGASGQRGAGGPEGAEGLPGTQGPSGATGNPGSRGLTGPEGSQGDAGREGPPGQMGPPGREGIDGEPGDSGERGFTGKPGPEGPQGPVGMYGFHGPAGERGRTGYPGEKGELGPPGIQGPPGKVGLKGPPGPPGERGPPGPQGRLGEPGIQGLPGLQGPAGNPGPDGEPGKGGRPGETGPAGSIGDPGSPGYQGRDGAAGAEGPPGEKGEKGEIGPVGDQGVSGLDGEQGEPGSTGHEGSVGHKGDQGDPGPPGNPGLNGPVGDLGARGLQGLVGPPGEPGKEGDVGEIGDVGDHGPQGEKGDKGPLGSTGNPGPGGIIGERGNRGEKGDKGLSGPLGPPGLIGSTGPSGLQGEMGNVGKQGSPGLDGPQGLKGETGSTGETGQLGPPGPMGPPGKKGEKGERGQTGRPGTEGPRGINGNQGPQGFIGQEGLKGEDGSPGNTGILGVTGPRGESGPIGAPGREGKEGEKGQPGEHGVPGKPGPPGNPGQEGNVGQRGEKGDSGIDGRPGRAGNPGRKGRRGKAGPRPPRGPAGLKGLQGDKGSEGPEGPRGPYGIPGLRGTKGEPGRRGIKGELGLDGPIGLQGEEGLKGPQGPTGERGPFGIKGEQGIEGPPGSQGLPGPQGAPGPPGIEGHKGFSGSEGPRGPPGPPGLPGAPGLPGPSLNLTAQSLKRTFFHAGALSVEGPWDLLHRLEEDLKHLRSPPNGSKENPASTCKELQLVQPHLSDGHYYVDPNQGSPQDSFLVFCNFTAGGETCLLPEESQIPVRPWLQDYQASEGFQWFSTLPGGFMFRYSETGVVQLRFLRLQSSAVHQGVSYVCQGQPGVAAKDKNVKFLTDTRDQSFLVTLQGCEWEETSNRTGTLLHFAPSDLSLLPLRDLAMLHDGDIGHQFGFLVGPTCYS

Target ORF information:

RefSeq Version	XM_018090175.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090175.1

ORF Insert Sequence:

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCGAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGTT GGATGTGGGC GGAGACTGGA CTACAGATCA TGGCAAAAAC 
TACACCCGTC AATCAACCAT ACTTGAGATT TCCCCAACAA ACACGAGTGG GAAGGTCCCC 
CAAGGACGGG GCGTCGGGAC ACAACATAGG GGCAGTCTGG ACTCTCAGCG GCTCATCAAC 
GAAGTGGATC TGTTGCTTCT GGAAGAGGAA GGCTTCATTC CCTCCTTCGT CGGGGGCGAG 
CAGGACGGCG CCAAGGCGGC CAAGAAGGAG GGACAACACA AAAAACTGGA CCCCGCTGAA 
GAGAACATCA CTATAGAGAA GCAAGAAAAG GTCCCCGAGC GCCTTCCGAC TGACAAATCC 
AGTAGTGACC TCCTAGACTT GGCCAAAGAC CCCAAACCGA TTCCATGGTC TACACCTGCT 
TCCACCAGTG CCCCCTTCCA CTCCACGGCC GTTCCAGATC TCTCTTTAGA AACAAAGAAC 
AAGACAAGTA TAGACATGGC ACCTACTCCG TCCCATGCCA CCGATCCGCA GAAGGCTGCT 
GGGAACGCAA GCCGCGAGAG CAACGGCATC CAGACAGGGA AACCCAAAAG ACCCGTTGCC 
CGTGGGCCTC CGGGACCCCG TGGTAGTCCT GGCTGCCCTG GTCGTCCTGG ATTTCCTGGA 
CCAAAAGGAG ACAAGGGCCA ACCGGGAATA AGGGGGCGTA CAGGAGTGAC TGGCAACCCC 
GGTCCCAACG GGCCCCCTGG ACTACCCACC GTAATGATCT GGAGCAACTC TAAAGAAGAC 
TGGGCAGCTT TCTATCAAAC GTCGTTCTAT CAGCTGCTGC ACGCAGGCTG GCCTAGAATC 
CGTGGGTCGA CCGGCTTCCC TGGGATGCCC GGAACGCCAG GAACTCCAGG TTCAATGGGA 
CCCCCGGGGC GACCTGGAAT CAAAGGACAG CAAGGTTCCA TGGGGACCCT GGGGCCCAAG 
GGTATGCAAG GGATGCCGGG GACACCAGGA AGGGACGGAT CTCCGGGATT AGACGGGAAG 
CCGGGATCTC CGGGGACGAC AGGAGCCAAA GGCCCTCAAG GATTTAAAGG GGACCAAGGA 
CCTCTCGGCG AGAAAGGAGA AGAAGGATTT CAAGGATCTC CAGGGCCAAG TGGTGAGGAA 
GGAGTCAAAG GAGCCAAGGG GGTGAAGGGA GAGGCCGGTC CCGTGGGTCC TTCGGGGGTT 
CAGGGCCCAA TGGGTGCTAA AGGTTCTTTG GGATTTCCAG GCCCCCCAGG ACCAATAGGA 
GATATGGGAA TTCCTGGAAC TCCTGGTCCA CCGGGGCCTG AGGGAGAACA AGGACAGCCA 
GGAACCCCAG GAACCCAGGG CTTGAATGGG TCCCAGGGTG ATCTGGGTCC TCCGGGAATA 
ATTGGTCATC GGGGACCACA GGGTCCTCCT GGAATAGAAG GTATAAGAGG ACCTCCTGGG 
CCCAGAGGAC CACAGGGATT GATGGGAGCC GATGGACCCC AAGGTCCACA AGGAAGCACA 
GGGGACCCTG GGCCAATGGG AGTAAGAGGA GAACGGGGAC TTGAAGGACC CATGGGATTG 
ATTGGATGGC CAGGACCAGA TGGACCTCCT GGTGATAAGG GTCGCCCTGC TGCAGATGCC 
GAAAAAGGAG ATCCTGGACC AAAGGGGGAA AAAGGTTGTC CAGGATCTCC TGGCATCAAA 
GGTCCTTCTG GAGAGCAAGG CCATGTTGGT TTTGTGGGTT TCCCAGGACC TAAAGGTCAG 
ATGGGCCCCA TCGGAGCTCC AGGGGATGAA GGCCAGGTTG GAGCTTCAGG TCTCAACGGC 
AGCGACGGAC CAAAGGGAAA CCGGGGACCT GATGGGCCAA AGGGGGTAAC AGGACCAAGA 
GGAAATCAGG GTCGTATGGG CCAGAGAGGA CTTGTAGGAA TGCCAGGAGC ATCAGGTCAG 
CGAGGAGCCG GGGGCCCCGA GGGAGCAGAA GGACTTCCTG GTACACAGGG ACCCAGTGGC 
GCAACAGGGA ATCCTGGGAG TAGGGGGCTT ACGGGTCCTG AAGGGTCTCA AGGGGACGCT 
GGAAGAGAAG GACCTCCAGG CCAAATGGGG CCTCCTGGGA GAGAAGGGAT TGATGGAGAA 
CCAGGAGATT CTGGCGAAAG GGGATTTACA GGGAAACCAG GCCCAGAAGG GCCACAGGGG 
CCAGTAGGCA TGTATGGGTT CCATGGCCCT GCTGGAGAGA GAGGTCGGAC GGGATACCCC 
GGGGAGAAGG GGGAATTGGG ACCTCCTGGG ATCCAAGGGC CACCAGGAAA AGTGGGACTA 
AAGGGTCCTC CTGGGCCACC AGGGGAAAGA GGCCCACCAG GACCCCAGGG TCGACTAGGA 
GAACCAGGCA TCCAGGGTTT GCCCGGCCTT CAGGGCCCAG CTGGTAACCC GGGGCCAGAT 
GGGGAGCCTG GAAAGGGTGG ACGTCCAGGT GAAACCGGGC CTGCTGGGAG TATAGGAGAC 
CCAGGATCTC CAGGATACCA GGGTCGAGAC GGAGCAGCAG GTGCTGAAGG ACCCCCAGGA 
GAGAAAGGAG AAAAGGGAGA AATTGGGCCT GTAGGAGACC AAGGGGTGAG CGGTTTGGAC 
GGGGAGCAGG GAGAACCTGG GTCTACTGGA CATGAAGGAT CAGTTGGCCA TAAAGGAGAT 
CAGGGGGACC CTGGTCCTCC AGGGAATCCT GGTCTAAACG GGCCTGTAGG AGACCTAGGG 
GCAAGAGGAC TGCAGGGCCT GGTGGGACCC CCAGGGGAAC CAGGGAAGGA GGGAGATGTG 
GGGGAAATTG GAGATGTTGG AGACCACGGT CCTCAAGGAG AAAAGGGAGA CAAAGGGCCA 
CTGGGTAGTA CCGGGAACCC AGGACCTGGC GGCATTATAG GAGAACGAGG CAATCGAGGT 
GAAAAGGGGG ACAAAGGCCT GAGTGGACCT CTGGGACCCC CGGGGTTGAT TGGATCTACA 
GGACCTTCTG GTCTACAGGG AGAAATGGGA AATGTTGGCA AACAAGGGTC CCCAGGTCTT 
GATGGACCTC AAGGTTTGAA GGGGGAAACT GGTTCCACTG GAGAGACTGG TCAACTCGGT 
CCCCCTGGGC CAATGGGCCC ACCTGGAAAG AAAGGGGAGA AGGGGGAGCG AGGACAGACT 
GGGAGACCAG GAACAGAGGG GCCACGAGGC ATTAACGGCA ACCAGGGGCC ACAAGGTTTT 
ATTGGCCAAG AGGGACTCAA GGGGGAAGAT GGGAGCCCGG GAAATACAGG AATCCTGGGG 
GTTACGGGGC CACGTGGGGA GTCTGGACCC ATAGGGGCAC CAGGAAGAGA AGGGAAGGAA 
GGAGAAAAGG GGCAACCAGG AGAACATGGA GTTCCAGGGA AACCGGGGCC CCCTGGAAAC 
CCAGGACAAG AAGGGAATGT GGGGCAGAGG GGAGAAAAGG GTGACAGTGG CATTGATGGC 
CGCCCTGGCA GAGCAGGGAA TCCTGGGAGA AAGGGCAGAA GAGGGAAAGC AGGTCCAAGG 
CCACCAAGAG GACCTGCAGG TCTCAAGGGT CTACAAGGAG ATAAGGGTTC GGAAGGACCA 
GAAGGACCCA GAGGACCATA TGGCATTCCT GGACTCAGAG GAACGAAAGG TGAACCGGGT 
CGAAGAGGTA TAAAGGGTGA GCTGGGCTTG GATGGACCAA TAGGACTGCA AGGAGAAGAA 
GGGCTGAAGG GACCACAAGG ACCGACCGGG GAGCGGGGAC CTTTCGGGAT CAAAGGGGAA 
CAGGGAATTG AGGGGCCACC TGGCTCTCAA GGATTACCTG GACCCCAAGG TGCACCAGGA 
CCACCAGGGA TAGAGGGACA TAAAGGATTC TCTGGATCTG AAGGGCCCAG AGGGCCACCT 
GGACCACCGG GGCTTCCTGG TGCTCCTGGA CTCCCAGGAC CTTCTCTAAA CTTAACGGCC 
CAAAGTTTAA AGCGGACCTT CTTCCATGCC GGGGCCTTGA GCGTAGAGGG ACCTTGGGAT 
CTTCTGCATC GTTTGGAAGA GGACTTGAAA CACTTGCGCT CTCCGCCCAA TGGTTCCAAA 
GAAAACCCAG CCAGCACCTG TAAGGAACTT CAGCTGGTGC AACCTCATCT GTCGGATGGT 
CATTACTATG TAGATCCCAA CCAGGGGAGT CCACAGGATT CTTTCCTTGT GTTCTGTAAC 
TTCACTGCAG GCGGTGAGAC GTGTCTGCTC CCTGAGGAGA GCCAAATACC TGTCAGACCT 
TGGCTTCAGG ACTATCAGGC TTCTGAGGGT TTCCAGTGGT TCAGCACTCT TCCTGGGGGC 
TTTATGTTCA GGTACAGTGA AACAGGAGTC GTCCAGCTGC GGTTCCTGAG GCTACAGAGC 
AGCGCCGTAC ATCAGGGGGT GAGCTACGTG TGCCAAGGTC AGCCCGGCGT CGCTGCGAAA 
GACAAGAATG TGAAGTTTCT GACCGATACC CGGGATCAGA GCTTTCTAGT GACTCTGCAG 
GGCTGTGAGT GGGAAGAGAC TTCAAACAGA ACAGGAACTC TCCTCCACTT TGCCCCCTCA 
GACCTCAGCC TCCTTCCTCT CAGAGACTTG GCCATGTTAC ACGATGGGGA CATTGGGCAC 
CAGTTTGGTT TCTTGGTGGG TCCTACCTGC TACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495290 ( XM_018090176.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945665.1 Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X2, mRNA.

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CloneID	OXa41236
Clone ID Related Accession (Same CDS sequence)	XM_018090176.1
Accession Version	XM_018090176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(I) chain-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683627.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCGAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGTT GGATGTGGGC GGAGACTGGA CTACAGATCA TGGCAAAAAC 
TACACCCGTC AATCAACCAT ACTTGAGATT TCCCCAACAA ACACGAGTGG GAAGGTCCCC 
CAAGGACGGG GCGTCGGGAC ACAACATAGG GGCAGTCTGG ACTCTCAGCG GCTCATCAAC 
GAAGTGGATC TGTTGCTTCT GGAAGAGGAA GGCTTCATTC CCTCCTTCGT CGGGGGCGAG 
CAGGACGGCG CCAAGGCGGC CAAGAAGGAG GGACAACACA AAAAACTGGA CCCCGCTGAA 
GAGAACATCA CTATAGAGAA GCAAGAAAAG GTCCCCGAGC GCCTTCCGAC TGACAAATCC 
AGTAGTGACC TCCTAGACTT GGCCAAAGAC CCCAAACCGA TTCCATGGTC TACACCTGCT 
TCCACCAGTG CCCCCTTCCA CTCCACGGCC GTTCCAGATC TCTCTTTAGA AACAAAGAAC 
AAGACAAGTA TAGACATGGC ACCTACTCCG TCCCATGCCA CCGATCCGCA GAAGGCTGCT 
GGGAACGCAA GCCGCGAGAG CAACGGCATC CAGACAGGGA AACCCAAAAG ACCCGTTGCC 
CGTGGGCCTC CGGGACCCCG TGGTAGTCCT GGCTGCCCTG GTCGTCCTGG ATTTCCTGGA 
CCAAAAGGAG ACAAGGGCCA ACCGGGAATA AGGGGGCGTA CAGGAGTGAC TGGCAACCCC 
GGTCCCAACG GGCCCCCTGG ACTACCCACC GTAATGATCT GGAGCAACTC TAAAGAAGAC 
TGGGCAGCTT TCTATCAAAC GTCGTTCTAT CAGCTGCTGC ACGCAGGCTG GCCTAGAATC 
CGTGGGTCGA CCGGCTTCCC TGGGATGCCC GGAACGCCAG GAACTCCAGG TTCAATGGGA 
CCCCCGGGGC GACCTGGAAT CAAAGGACAG CAAGGTTCCA TGGGGACCCT GGGGCCCAAG 
GGTATGCAAG GGATGCCGGG GACACCAGGA AGGGACGGAT CTCCGGGATT AGACGGGAAG 
CCGGGATCTC CGGGGACGAC AGGAGCCAAA GGCCCTCAAG GATTTAAAGG GGACCAAGGA 
CCTCTCGGCG AGAAAGGAGA AGAAGGATTT CAAGGATCTC CAGGGCCAAG TGGTGAGGAA 
GGAGTCAAAG GAGCCAAGGG GGTGAAGGGA GAGGCCGGTC CCGTGGGTCC TTCGGGGGTT 
CAGGGCCCAA TGGGTGCTAA AGGTTCTTTG GGATTTCCAG GCCCCCCAGG ACCAATAGGA 
GATATGGGAA TTCCTGGAAC TCCTGGTCCA CCGGGGCCTG AGGGAGAACA AGGACAGCCA 
GGAACCCCAG GAACCCAGGG CTTGAATGGG TCCCAGGGTG ATCTGGGTCC TCCGGGAATA 
ATTGGTCATC GGGGACCACA GGGATTGATG GGAGCCGATG GACCCCAAGG TCCACAAGGA 
AGCACAGGGG ACCCTGGGCC AATGGGAGTA AGAGGAGAAC GGGGACTTGA AGGACCCATG 
GGATTGATTG GATGGCCAGG ACCAGATGGA CCTCCTGGTG ATAAGGGTCG CCCTGCTGCA 
GATGCCGAAA AAGGAGATCC TGGACCAAAG GGGGAAAAAG GTTGTCCAGG ATCTCCTGGC 
ATCAAAGGTC CTTCTGGAGA GCAAGGCCAT GTTGGTTTTG TGGGTTTCCC AGGACCTAAA 
GGTCAGATGG GCCCCATCGG AGCTCCAGGG GATGAAGGCC AGGTTGGAGC TTCAGGTCTC 
AACGGCAGCG ACGGACCAAA GGGAAACCGG GGACCTGATG GGCCAAAGGG GGTAACAGGA 
CCAAGAGGAA ATCAGGGTCG TATGGGCCAG AGAGGACTTG TAGGAATGCC AGGAGCATCA 
GGTCAGCGAG GAGCCGGGGG CCCCGAGGGA GCAGAAGGAC TTCCTGGTAC ACAGGGACCC 
AGTGGCGCAA CAGGGAATCC TGGGAGTAGG GGGCTTACGG GTCCTGAAGG GTCTCAAGGG 
GACGCTGGAA GAGAAGGACC TCCAGGCCAA ATGGGGCCTC CTGGGAGAGA AGGGATTGAT 
GGAGAACCAG GAGATTCTGG CGAAAGGGGA TTTACAGGGA AACCAGGCCC AGAAGGGCCA 
CAGGGGCCAG TAGGCATGTA TGGGTTCCAT GGCCCTGCTG GAGAGAGAGG TCGGACGGGA 
TACCCCGGGG AGAAGGGGGA ATTGGGACCT CCTGGGATCC AAGGGCCACC AGGAAAAGTG 
GGACTAAAGG GTCCTCCTGG GCCACCAGGG GAAAGAGGCC CACCAGGACC CCAGGGTCGA 
CTAGGAGAAC CAGGCATCCA GGGTTTGCCC GGCCTTCAGG GCCCAGCTGG TAACCCGGGG 
CCAGATGGGG AGCCTGGAAA GGGTGGACGT CCAGGTGAAA CCGGGCCTGC TGGGAGTATA 
GGAGACCCAG GATCTCCAGG ATACCAGGGT CGAGACGGAG CAGCAGGTGC TGAAGGACCC 
CCAGGAGAGA AAGGAGAAAA GGGAGAAATT GGGCCTGTAG GAGACCAAGG GGTGAGCGGT 
TTGGACGGGG AGCAGGGAGA ACCTGGGTCT ACTGGACATG AAGGATCAGT TGGCCATAAA 
GGAGATCAGG GGGACCCTGG TCCTCCAGGG AATCCTGGTC TAAACGGGCC TGTAGGAGAC 
CTAGGGGCAA GAGGACTGCA GGGCCTGGTG GGACCCCCAG GGGAACCAGG GAAGGAGGGA 
GATGTGGGGG AAATTGGAGA TGTTGGAGAC CACGGTCCTC AAGGAGAAAA GGGAGACAAA 
GGGCCACTGG GTAGTACCGG GAACCCAGGA CCTGGCGGCA TTATAGGAGA ACGAGGCAAT 
CGAGGTGAAA AGGGGGACAA AGGCCTGAGT GGACCTCTGG GACCCCCGGG GTTGATTGGA 
TCTACAGGAC CTTCTGGTCT ACAGGGAGAA ATGGGAAATG TTGGCAAACA AGGGTCCCCA 
GGTCTTGATG GACCTCAAGG TTTGAAGGGG GAAACTGGTT CCACTGGAGA GACTGGTCAA 
CTCGGTCCCC CTGGGCCAAT GGGCCCACCT GGAAAGAAAG GGGAGAAGGG GGAGCGAGGA 
CAGACTGGGA GACCAGGAAC AGAGGGGCCA CGAGGCATTA ACGGCAACCA GGGGCCACAA 
GGTTTTATTG GCCAAGAGGG ACTCAAGGGG GAAGATGGGA GCCCGGGAAA TACAGGAATC 
CTGGGGGTTA CGGGGCCACG TGGGGAGTCT GGACCCATAG GGGCACCAGG AAGAGAAGGG 
AAGGAAGGAG AAAAGGGGCA ACCAGGAGAA CATGGAGTTC CAGGGAAACC GGGGCCCCCT 
GGAAACCCAG GACAAGAAGG GAATGTGGGG CAGAGGGGAG AAAAGGGTGA CAGTGGCATT 
GATGGCCGCC CTGGCAGAGC AGGGAATCCT GGGAGAAAGG GCAGAAGAGG GAAAGCAGGT 
CCAAGGCCAC CAAGAGGACC TGCAGGTCTC AAGGGTCTAC AAGGAGATAA GGGTTCGGAA 
GGACCAGAAG GACCCAGAGG ACCATATGGC ATTCCTGGAC TCAGAGGAAC GAAAGGTGAA 
CCGGGTCGAA GAGGTATAAA GGGTGAGCTG GGCTTGGATG GACCAATAGG ACTGCAAGGA 
GAAGAAGGGC TGAAGGGACC ACAAGGACCG ACCGGGGAGC GGGGACCTTT CGGGATCAAA 
GGGGAACAGG GAATTGAGGG GCCACCTGGC TCTCAAGGAT TACCTGGACC CCAAGGTGCA 
CCAGGACCAC CAGGGATAGA GGGACATAAA GGATTCTCTG GATCTGAAGG GCCCAGAGGG 
CCACCTGGAC CACCGGGGCT TCCTGGTGCT CCTGGACTCC CAGGACCTTC TCTAAACTTA 
ACGGCCCAAA GTTTAAAGCG GACCTTCTTC CATGCCGGGG CCTTGAGCGT AGAGGGACCT 
TGGGATCTTC TGCATCGTTT GGAAGAGGAC TTGAAACACT TGCGCTCTCC GCCCAATGGT 
TCCAAAGAAA ACCCAGCCAG CACCTGTAAG GAACTTCAGC TGGTGCAACC TCATCTGTCG 
GATGGTCATT ACTATGTAGA TCCCAACCAG GGGAGTCCAC AGGATTCTTT CCTTGTGTTC 
TGTAACTTCA CTGCAGGCGG TGAGACGTGT CTGCTCCCTG AGGAGAGCCA AATACCTGTC 
AGACCTTGGC TTCAGGACTA TCAGGCTTCT GAGGGTTTCC AGTGGTTCAG CACTCTTCCT 
GGGGGCTTTA TGTTCAGGTA CAGTGAAACA GGAGTCGTCC AGCTGCGGTT CCTGAGGCTA 
CAGAGCAGCG CCGTACATCA GGGGGTGAGC TACGTGTGCC AAGGTCAGCC CGGCGTCGCT 
GCGAAAGACA AGAATGTGAA GTTTCTGACC GATACCCGGG ATCAGAGCTT TCTAGTGACT 
CTGCAGGGCT GTGAGTGGGA AGAGACTTCA AACAGAACAG GAACTCTCCT CCACTTTGCC 
CCCTCAGACC TCAGCCTCCT TCCTCTCAGA GACTTGGCCA TGTTACACGA TGGGGACATT 
GGGCACCAGT TTGGTTTCTT GGTGGGTCCT ACCTGCTACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945665.1
CDS	58..5382
Translation	MGRGGLSAAAIRTLGHRWALLLTLLLYRGAAGEQGDTPEAGSGQMAEEVDVLESLTLHSAELVNLTLTSEDSCLALELGQYSTLSISTMTAFGDSFADELTVLMMLRSSLREDTGLLSILSLHGLILFQIRINPKAFIFISSRRGHYEFSVPTLTDGQWHRVALSISSKGVALYVDCKLVEMLSWTNYFGLGVSTDGILMLGGLIEPYEVPFKGSLQQLVFVMGEPGAAKLYCRSYNQTCLSTFSEEHFFNLSQGDLRRREARVTKLDVGGDWTTDHGKNYTRQSTILEISPTNTSGKVPQGRGVGTQHRGSLDSQRLINEVDLLLLEEEGFIPSFVGGEQDGAKAAKKEGQHKKLDPAEENITIEKQEKVPERLPTDKSSSDLLDLAKDPKPIPWSTPASTSAPFHSTAVPDLSLETKNKTSIDMAPTPSHATDPQKAAGNASRESNGIQTGKPKRPVARGPPGPRGSPGCPGRPGFPGPKGDKGQPGIRGRTGVTGNPGPNGPPGLPTVMIWSNSKEDWAAFYQTSFYQLLHAGWPRIRGSTGFPGMPGTPGTPGSMGPPGRPGIKGQQGSMGTLGPKGMQGMPGTPGRDGSPGLDGKPGSPGTTGAKGPQGFKGDQGPLGEKGEEGFQGSPGPSGEEGVKGAKGVKGEAGPVGPSGVQGPMGAKGSLGFPGPPGPIGDMGIPGTPGPPGPEGEQGQPGTPGTQGLNGSQGDLGPPGIIGHRGPQGLMGADGPQGPQGSTGDPGPMGVRGERGLEGPMGLIGWPGPDGPPGDKGRPAADAEKGDPGPKGEKGCPGSPGIKGPSGEQGHVGFVGFPGPKGQMGPIGAPGDEGQVGASGLNGSDGPKGNRGPDGPKGVTGPRGNQGRMGQRGLVGMPGASGQRGAGGPEGAEGLPGTQGPSGATGNPGSRGLTGPEGSQGDAGREGPPGQMGPPGREGIDGEPGDSGERGFTGKPGPEGPQGPVGMYGFHGPAGERGRTGYPGEKGELGPPGIQGPPGKVGLKGPPGPPGERGPPGPQGRLGEPGIQGLPGLQGPAGNPGPDGEPGKGGRPGETGPAGSIGDPGSPGYQGRDGAAGAEGPPGEKGEKGEIGPVGDQGVSGLDGEQGEPGSTGHEGSVGHKGDQGDPGPPGNPGLNGPVGDLGARGLQGLVGPPGEPGKEGDVGEIGDVGDHGPQGEKGDKGPLGSTGNPGPGGIIGERGNRGEKGDKGLSGPLGPPGLIGSTGPSGLQGEMGNVGKQGSPGLDGPQGLKGETGSTGETGQLGPPGPMGPPGKKGEKGERGQTGRPGTEGPRGINGNQGPQGFIGQEGLKGEDGSPGNTGILGVTGPRGESGPIGAPGREGKEGEKGQPGEHGVPGKPGPPGNPGQEGNVGQRGEKGDSGIDGRPGRAGNPGRKGRRGKAGPRPPRGPAGLKGLQGDKGSEGPEGPRGPYGIPGLRGTKGEPGRRGIKGELGLDGPIGLQGEEGLKGPQGPTGERGPFGIKGEQGIEGPPGSQGLPGPQGAPGPPGIEGHKGFSGSEGPRGPPGPPGLPGAPGLPGPSLNLTAQSLKRTFFHAGALSVEGPWDLLHRLEEDLKHLRSPPNGSKENPASTCKELQLVQPHLSDGHYYVDPNQGSPQDSFLVFCNFTAGGETCLLPEESQIPVRPWLQDYQASEGFQWFSTLPGGFMFRYSETGVVQLRFLRLQSSAVHQGVSYVCQGQPGVAAKDKNVKFLTDTRDQSFLVTLQGCEWEETSNRTGTLLHFAPSDLSLLPLRDLAMLHDGDIGHQFGFLVGPTCYS

Target ORF information:

RefSeq Version	XM_018090176.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090176.1

ORF Insert Sequence:

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCGAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGTT GGATGTGGGC GGAGACTGGA CTACAGATCA TGGCAAAAAC 
TACACCCGTC AATCAACCAT ACTTGAGATT TCCCCAACAA ACACGAGTGG GAAGGTCCCC 
CAAGGACGGG GCGTCGGGAC ACAACATAGG GGCAGTCTGG ACTCTCAGCG GCTCATCAAC 
GAAGTGGATC TGTTGCTTCT GGAAGAGGAA GGCTTCATTC CCTCCTTCGT CGGGGGCGAG 
CAGGACGGCG CCAAGGCGGC CAAGAAGGAG GGACAACACA AAAAACTGGA CCCCGCTGAA 
GAGAACATCA CTATAGAGAA GCAAGAAAAG GTCCCCGAGC GCCTTCCGAC TGACAAATCC 
AGTAGTGACC TCCTAGACTT GGCCAAAGAC CCCAAACCGA TTCCATGGTC TACACCTGCT 
TCCACCAGTG CCCCCTTCCA CTCCACGGCC GTTCCAGATC TCTCTTTAGA AACAAAGAAC 
AAGACAAGTA TAGACATGGC ACCTACTCCG TCCCATGCCA CCGATCCGCA GAAGGCTGCT 
GGGAACGCAA GCCGCGAGAG CAACGGCATC CAGACAGGGA AACCCAAAAG ACCCGTTGCC 
CGTGGGCCTC CGGGACCCCG TGGTAGTCCT GGCTGCCCTG GTCGTCCTGG ATTTCCTGGA 
CCAAAAGGAG ACAAGGGCCA ACCGGGAATA AGGGGGCGTA CAGGAGTGAC TGGCAACCCC 
GGTCCCAACG GGCCCCCTGG ACTACCCACC GTAATGATCT GGAGCAACTC TAAAGAAGAC 
TGGGCAGCTT TCTATCAAAC GTCGTTCTAT CAGCTGCTGC ACGCAGGCTG GCCTAGAATC 
CGTGGGTCGA CCGGCTTCCC TGGGATGCCC GGAACGCCAG GAACTCCAGG TTCAATGGGA 
CCCCCGGGGC GACCTGGAAT CAAAGGACAG CAAGGTTCCA TGGGGACCCT GGGGCCCAAG 
GGTATGCAAG GGATGCCGGG GACACCAGGA AGGGACGGAT CTCCGGGATT AGACGGGAAG 
CCGGGATCTC CGGGGACGAC AGGAGCCAAA GGCCCTCAAG GATTTAAAGG GGACCAAGGA 
CCTCTCGGCG AGAAAGGAGA AGAAGGATTT CAAGGATCTC CAGGGCCAAG TGGTGAGGAA 
GGAGTCAAAG GAGCCAAGGG GGTGAAGGGA GAGGCCGGTC CCGTGGGTCC TTCGGGGGTT 
CAGGGCCCAA TGGGTGCTAA AGGTTCTTTG GGATTTCCAG GCCCCCCAGG ACCAATAGGA 
GATATGGGAA TTCCTGGAAC TCCTGGTCCA CCGGGGCCTG AGGGAGAACA AGGACAGCCA 
GGAACCCCAG GAACCCAGGG CTTGAATGGG TCCCAGGGTG ATCTGGGTCC TCCGGGAATA 
ATTGGTCATC GGGGACCACA GGGATTGATG GGAGCCGATG GACCCCAAGG TCCACAAGGA 
AGCACAGGGG ACCCTGGGCC AATGGGAGTA AGAGGAGAAC GGGGACTTGA AGGACCCATG 
GGATTGATTG GATGGCCAGG ACCAGATGGA CCTCCTGGTG ATAAGGGTCG CCCTGCTGCA 
GATGCCGAAA AAGGAGATCC TGGACCAAAG GGGGAAAAAG GTTGTCCAGG ATCTCCTGGC 
ATCAAAGGTC CTTCTGGAGA GCAAGGCCAT GTTGGTTTTG TGGGTTTCCC AGGACCTAAA 
GGTCAGATGG GCCCCATCGG AGCTCCAGGG GATGAAGGCC AGGTTGGAGC TTCAGGTCTC 
AACGGCAGCG ACGGACCAAA GGGAAACCGG GGACCTGATG GGCCAAAGGG GGTAACAGGA 
CCAAGAGGAA ATCAGGGTCG TATGGGCCAG AGAGGACTTG TAGGAATGCC AGGAGCATCA 
GGTCAGCGAG GAGCCGGGGG CCCCGAGGGA GCAGAAGGAC TTCCTGGTAC ACAGGGACCC 
AGTGGCGCAA CAGGGAATCC TGGGAGTAGG GGGCTTACGG GTCCTGAAGG GTCTCAAGGG 
GACGCTGGAA GAGAAGGACC TCCAGGCCAA ATGGGGCCTC CTGGGAGAGA AGGGATTGAT 
GGAGAACCAG GAGATTCTGG CGAAAGGGGA TTTACAGGGA AACCAGGCCC AGAAGGGCCA 
CAGGGGCCAG TAGGCATGTA TGGGTTCCAT GGCCCTGCTG GAGAGAGAGG TCGGACGGGA 
TACCCCGGGG AGAAGGGGGA ATTGGGACCT CCTGGGATCC AAGGGCCACC AGGAAAAGTG 
GGACTAAAGG GTCCTCCTGG GCCACCAGGG GAAAGAGGCC CACCAGGACC CCAGGGTCGA 
CTAGGAGAAC CAGGCATCCA GGGTTTGCCC GGCCTTCAGG GCCCAGCTGG TAACCCGGGG 
CCAGATGGGG AGCCTGGAAA GGGTGGACGT CCAGGTGAAA CCGGGCCTGC TGGGAGTATA 
GGAGACCCAG GATCTCCAGG ATACCAGGGT CGAGACGGAG CAGCAGGTGC TGAAGGACCC 
CCAGGAGAGA AAGGAGAAAA GGGAGAAATT GGGCCTGTAG GAGACCAAGG GGTGAGCGGT 
TTGGACGGGG AGCAGGGAGA ACCTGGGTCT ACTGGACATG AAGGATCAGT TGGCCATAAA 
GGAGATCAGG GGGACCCTGG TCCTCCAGGG AATCCTGGTC TAAACGGGCC TGTAGGAGAC 
CTAGGGGCAA GAGGACTGCA GGGCCTGGTG GGACCCCCAG GGGAACCAGG GAAGGAGGGA 
GATGTGGGGG AAATTGGAGA TGTTGGAGAC CACGGTCCTC AAGGAGAAAA GGGAGACAAA 
GGGCCACTGG GTAGTACCGG GAACCCAGGA CCTGGCGGCA TTATAGGAGA ACGAGGCAAT 
CGAGGTGAAA AGGGGGACAA AGGCCTGAGT GGACCTCTGG GACCCCCGGG GTTGATTGGA 
TCTACAGGAC CTTCTGGTCT ACAGGGAGAA ATGGGAAATG TTGGCAAACA AGGGTCCCCA 
GGTCTTGATG GACCTCAAGG TTTGAAGGGG GAAACTGGTT CCACTGGAGA GACTGGTCAA 
CTCGGTCCCC CTGGGCCAAT GGGCCCACCT GGAAAGAAAG GGGAGAAGGG GGAGCGAGGA 
CAGACTGGGA GACCAGGAAC AGAGGGGCCA CGAGGCATTA ACGGCAACCA GGGGCCACAA 
GGTTTTATTG GCCAAGAGGG ACTCAAGGGG GAAGATGGGA GCCCGGGAAA TACAGGAATC 
CTGGGGGTTA CGGGGCCACG TGGGGAGTCT GGACCCATAG GGGCACCAGG AAGAGAAGGG 
AAGGAAGGAG AAAAGGGGCA ACCAGGAGAA CATGGAGTTC CAGGGAAACC GGGGCCCCCT 
GGAAACCCAG GACAAGAAGG GAATGTGGGG CAGAGGGGAG AAAAGGGTGA CAGTGGCATT 
GATGGCCGCC CTGGCAGAGC AGGGAATCCT GGGAGAAAGG GCAGAAGAGG GAAAGCAGGT 
CCAAGGCCAC CAAGAGGACC TGCAGGTCTC AAGGGTCTAC AAGGAGATAA GGGTTCGGAA 
GGACCAGAAG GACCCAGAGG ACCATATGGC ATTCCTGGAC TCAGAGGAAC GAAAGGTGAA 
CCGGGTCGAA GAGGTATAAA GGGTGAGCTG GGCTTGGATG GACCAATAGG ACTGCAAGGA 
GAAGAAGGGC TGAAGGGACC ACAAGGACCG ACCGGGGAGC GGGGACCTTT CGGGATCAAA 
GGGGAACAGG GAATTGAGGG GCCACCTGGC TCTCAAGGAT TACCTGGACC CCAAGGTGCA 
CCAGGACCAC CAGGGATAGA GGGACATAAA GGATTCTCTG GATCTGAAGG GCCCAGAGGG 
CCACCTGGAC CACCGGGGCT TCCTGGTGCT CCTGGACTCC CAGGACCTTC TCTAAACTTA 
ACGGCCCAAA GTTTAAAGCG GACCTTCTTC CATGCCGGGG CCTTGAGCGT AGAGGGACCT 
TGGGATCTTC TGCATCGTTT GGAAGAGGAC TTGAAACACT TGCGCTCTCC GCCCAATGGT 
TCCAAAGAAA ACCCAGCCAG CACCTGTAAG GAACTTCAGC TGGTGCAACC TCATCTGTCG 
GATGGTCATT ACTATGTAGA TCCCAACCAG GGGAGTCCAC AGGATTCTTT CCTTGTGTTC 
TGTAACTTCA CTGCAGGCGG TGAGACGTGT CTGCTCCCTG AGGAGAGCCA AATACCTGTC 
AGACCTTGGC TTCAGGACTA TCAGGCTTCT GAGGGTTTCC AGTGGTTCAG CACTCTTCCT 
GGGGGCTTTA TGTTCAGGTA CAGTGAAACA GGAGTCGTCC AGCTGCGGTT CCTGAGGCTA 
CAGAGCAGCG CCGTACATCA GGGGGTGAGC TACGTGTGCC AAGGTCAGCC CGGCGTCGCT 
GCGAAAGACA AGAATGTGAA GTTTCTGACC GATACCCGGG ATCAGAGCTT TCTAGTGACT 
CTGCAGGGCT GTGAGTGGGA AGAGACTTCA AACAGAACAG GAACTCTCCT CCACTTTGCC 
CCCTCAGACC TCAGCCTCCT TCCTCTCAGA GACTTGGCCA TGTTACACGA TGGGGACATT 
GGGCACCAGT TTGGTTTCTT GGTGGGTCCT ACCTGCTACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495290 ( XM_018090177.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945666.1 Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X3, mRNA.

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CloneID	OXa41237
Clone ID Related Accession (Same CDS sequence)	XM_018090177.1
Accession Version	XM_018090177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(I) chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683627.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCGAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGAT TTCCCCAACA AACACGAGTG GGAAGGTCCC CCAAGGACGG 
GGCGTCGGGA CACAACATAG GGGCAGTCTG GACTCTCAGC GGCTCATCAA CGAAGTGGAT 
CTGTTGCTTC TGGAAGAGGA AGGCTTCATT CCCTCCTTCG TCGGGGGCGA GCAGGACGGC 
GCCAAGGCGG CCAAGAAGGA GGGACAACAC AAAAAACTGG ACCCCGCTGA AGAGAACATC 
ACTATAGAGA AGCAAGAAAA GGTCCCCGAG CGCCTTCCGA CTGACAAATC CAGTAGTGAC 
CTCCTAGACT TGGCCAAAGA CCCCAAACCG ATTCCATGGT CTACACCTGC TTCCACCAGT 
GCCCCCTTCC ACTCCACGGC CGTTCCAGAT CTCTCTTTAG AAACAAAGAA CAAGACAAGT 
ATAGACATGG CACCTACTCC GTCCCATGCC ACCGATCCGC AGAAGGCTGC TGGGAACGCA 
AGCCGCGAGA GCAACGGCAT CCAGACAGGG AAACCCAAAA GACCCGTTGC CCGTGGGCCT 
CCGGGACCCC GTGGTAGTCC TGGCTGCCCT GGTCGTCCTG GATTTCCTGG ACCAAAAGGA 
GACAAGGGCC AACCGGGAAT AAGGGGGCGT ACAGGAGTGA CTGGCAACCC CGGTCCCAAC 
GGGCCCCCTG GACTACCCAC CGTAATGATC TGGAGCAACT CTAAAGAAGA CTGGGCAGCT 
TTCTATCAAA CGTCGTTCTA TCAGCTGCTG CACGCAGGCT GGCCTAGAAT CCGTGGGTCG 
ACCGGCTTCC CTGGGATGCC CGGAACGCCA GGAACTCCAG GTTCAATGGG ACCCCCGGGG 
CGACCTGGAA TCAAAGGACA GCAAGGTTCC ATGGGGACCC TGGGGCCCAA GGGTATGCAA 
GGGATGCCGG GGACACCAGG AAGGGACGGA TCTCCGGGAT TAGACGGGAA GCCGGGATCT 
CCGGGGACGA CAGGAGCCAA AGGCCCTCAA GGATTTAAAG GGGACCAAGG ACCTCTCGGC 
GAGAAAGGAG AAGAAGGATT TCAAGGATCT CCAGGGCCAA GTGGTGAGGA AGGAGTCAAA 
GGAGCCAAGG GGGTGAAGGG AGAGGCCGGT CCCGTGGGTC CTTCGGGGGT TCAGGGCCCA 
ATGGGTGCTA AAGGTTCTTT GGGATTTCCA GGCCCCCCAG GACCAATAGG AGATATGGGA 
ATTCCTGGAA CTCCTGGTCC ACCGGGGCCT GAGGGAGAAC AAGGACAGCC AGGAACCCCA 
GGAACCCAGG GCTTGAATGG GTCCCAGGGT GATCTGGGTC CTCCGGGAAT AATTGGTCAT 
CGGGGACCAC AGGGTCCTCC TGGAATAGAA GGTATAAGAG GACCTCCTGG GCCCAGAGGA 
CCACAGGGAT TGATGGGAGC CGATGGACCC CAAGGTCCAC AAGGAAGCAC AGGGGACCCT 
GGGCCAATGG GAGTAAGAGG AGAACGGGGA CTTGAAGGAC CCATGGGATT GATTGGATGG 
CCAGGACCAG ATGGACCTCC TGGTGATAAG GGTCGCCCTG CTGCAGATGC CGAAAAAGGA 
GATCCTGGAC CAAAGGGGGA AAAAGGTTGT CCAGGATCTC CTGGCATCAA AGGTCCTTCT 
GGAGAGCAAG GCCATGTTGG TTTTGTGGGT TTCCCAGGAC CTAAAGGTCA GATGGGCCCC 
ATCGGAGCTC CAGGGGATGA AGGCCAGGTT GGAGCTTCAG GTCTCAACGG CAGCGACGGA 
CCAAAGGGAA ACCGGGGACC TGATGGGCCA AAGGGGGTAA CAGGACCAAG AGGAAATCAG 
GGTCGTATGG GCCAGAGAGG ACTTGTAGGA ATGCCAGGAG CATCAGGTCA GCGAGGAGCC 
GGGGGCCCCG AGGGAGCAGA AGGACTTCCT GGTACACAGG GACCCAGTGG CGCAACAGGG 
AATCCTGGGA GTAGGGGGCT TACGGGTCCT GAAGGGTCTC AAGGGGACGC TGGAAGAGAA 
GGACCTCCAG GCCAAATGGG GCCTCCTGGG AGAGAAGGGA TTGATGGAGA ACCAGGAGAT 
TCTGGCGAAA GGGGATTTAC AGGGAAACCA GGCCCAGAAG GGCCACAGGG GCCAGTAGGC 
ATGTATGGGT TCCATGGCCC TGCTGGAGAG AGAGGTCGGA CGGGATACCC CGGGGAGAAG 
GGGGAATTGG GACCTCCTGG GATCCAAGGG CCACCAGGAA AAGTGGGACT AAAGGGTCCT 
CCTGGGCCAC CAGGGGAAAG AGGCCCACCA GGACCCCAGG GTCGACTAGG AGAACCAGGC 
ATCCAGGGTT TGCCCGGCCT TCAGGGCCCA GCTGGTAACC CGGGGCCAGA TGGGGAGCCT 
GGAAAGGGTG GACGTCCAGG TGAAACCGGG CCTGCTGGGA GTATAGGAGA CCCAGGATCT 
CCAGGATACC AGGGTCGAGA CGGAGCAGCA GGTGCTGAAG GACCCCCAGG AGAGAAAGGA 
GAAAAGGGAG AAATTGGGCC TGTAGGAGAC CAAGGGGTGA GCGGTTTGGA CGGGGAGCAG 
GGAGAACCTG GGTCTACTGG ACATGAAGGA TCAGTTGGCC ATAAAGGAGA TCAGGGGGAC 
CCTGGTCCTC CAGGGAATCC TGGTCTAAAC GGGCCTGTAG GAGACCTAGG GGCAAGAGGA 
CTGCAGGGCC TGGTGGGACC CCCAGGGGAA CCAGGGAAGG AGGGAGATGT GGGGGAAATT 
GGAGATGTTG GAGACCACGG TCCTCAAGGA GAAAAGGGAG ACAAAGGGCC ACTGGGTAGT 
ACCGGGAACC CAGGACCTGG CGGCATTATA GGAGAACGAG GCAATCGAGG TGAAAAGGGG 
GACAAAGGCC TGAGTGGACC TCTGGGACCC CCGGGGTTGA TTGGATCTAC AGGACCTTCT 
GGTCTACAGG GAGAAATGGG AAATGTTGGC AAACAAGGGT CCCCAGGTCT TGATGGACCT 
CAAGGTTTGA AGGGGGAAAC TGGTTCCACT GGAGAGACTG GTCAACTCGG TCCCCCTGGG 
CCAATGGGCC CACCTGGAAA GAAAGGGGAG AAGGGGGAGC GAGGACAGAC TGGGAGACCA 
GGAACAGAGG GGCCACGAGG CATTAACGGC AACCAGGGGC CACAAGGTTT TATTGGCCAA 
GAGGGACTCA AGGGGGAAGA TGGGAGCCCG GGAAATACAG GAATCCTGGG GGTTACGGGG 
CCACGTGGGG AGTCTGGACC CATAGGGGCA CCAGGAAGAG AAGGGAAGGA AGGAGAAAAG 
GGGCAACCAG GAGAACATGG AGTTCCAGGG AAACCGGGGC CCCCTGGAAA CCCAGGACAA 
GAAGGGAATG TGGGGCAGAG GGGAGAAAAG GGTGACAGTG GCATTGATGG CCGCCCTGGC 
AGAGCAGGGA ATCCTGGGAG AAAGGGCAGA AGAGGGAAAG CAGGTCCAAG GCCACCAAGA 
GGACCTGCAG GTCTCAAGGG TCTACAAGGA GATAAGGGTT CGGAAGGACC AGAAGGACCC 
AGAGGACCAT ATGGCATTCC TGGACTCAGA GGAACGAAAG GTGAACCGGG TCGAAGAGGT 
ATAAAGGGTG AGCTGGGCTT GGATGGACCA ATAGGACTGC AAGGAGAAGA AGGGCTGAAG 
GGACCACAAG GACCGACCGG GGAGCGGGGA CCTTTCGGGA TCAAAGGGGA ACAGGGAATT 
GAGGGGCCAC CTGGCTCTCA AGGATTACCT GGACCCCAAG GTGCACCAGG ACCACCAGGG 
ATAGAGGGAC ATAAAGGATT CTCTGGATCT GAAGGGCCCA GAGGGCCACC TGGACCACCG 
GGGCTTCCTG GTGCTCCTGG ACTCCCAGGA CCTTCTCTAA ACTTAACGGC CCAAAGTTTA 
AAGCGGACCT TCTTCCATGC CGGGGCCTTG AGCGTAGAGG GACCTTGGGA TCTTCTGCAT 
CGTTTGGAAG AGGACTTGAA ACACTTGCGC TCTCCGCCCA ATGGTTCCAA AGAAAACCCA 
GCCAGCACCT GTAAGGAACT TCAGCTGGTG CAACCTCATC TGTCGGATGG TCATTACTAT 
GTAGATCCCA ACCAGGGGAG TCCACAGGAT TCTTTCCTTG TGTTCTGTAA CTTCACTGCA 
GGCGGTGAGA CGTGTCTGCT CCCTGAGGAG AGCCAAATAC CTGTCAGACC TTGGCTTCAG 
GACTATCAGG CTTCTGAGGG TTTCCAGTGG TTCAGCACTC TTCCTGGGGG CTTTATGTTC 
AGGTACAGTG AAACAGGAGT CGTCCAGCTG CGGTTCCTGA GGCTACAGAG CAGCGCCGTA 
CATCAGGGGG TGAGCTACGT GTGCCAAGGT CAGCCCGGCG TCGCTGCGAA AGACAAGAAT 
GTGAAGTTTC TGACCGATAC CCGGGATCAG AGCTTTCTAG TGACTCTGCA GGGCTGTGAG 
TGGGAAGAGA CTTCAAACAG AACAGGAACT CTCCTCCACT TTGCCCCCTC AGACCTCAGC 
CTCCTTCCTC TCAGAGACTT GGCCATGTTA CACGATGGGG ACATTGGGCA CCAGTTTGGT 
TTCTTGGTGG GTCCTACCTG CTACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945666.1
CDS	58..5367
Translation	MGRGGLSAAAIRTLGHRWALLLTLLLYRGAAGEQGDTPEAGSGQMAEEVDVLESLTLHSAELVNLTLTSEDSCLALELGQYSTLSISTMTAFGDSFADELTVLMMLRSSLREDTGLLSILSLHGLILFQIRINPKAFIFISSRRGHYEFSVPTLTDGQWHRVALSISSKGVALYVDCKLVEMLSWTNYFGLGVSTDGILMLGGLIEPYEVPFKGSLQQLVFVMGEPGAAKLYCRSYNQTCLSTFSEEHFFNLSQGDLRRREARVTKISPTNTSGKVPQGRGVGTQHRGSLDSQRLINEVDLLLLEEEGFIPSFVGGEQDGAKAAKKEGQHKKLDPAEENITIEKQEKVPERLPTDKSSSDLLDLAKDPKPIPWSTPASTSAPFHSTAVPDLSLETKNKTSIDMAPTPSHATDPQKAAGNASRESNGIQTGKPKRPVARGPPGPRGSPGCPGRPGFPGPKGDKGQPGIRGRTGVTGNPGPNGPPGLPTVMIWSNSKEDWAAFYQTSFYQLLHAGWPRIRGSTGFPGMPGTPGTPGSMGPPGRPGIKGQQGSMGTLGPKGMQGMPGTPGRDGSPGLDGKPGSPGTTGAKGPQGFKGDQGPLGEKGEEGFQGSPGPSGEEGVKGAKGVKGEAGPVGPSGVQGPMGAKGSLGFPGPPGPIGDMGIPGTPGPPGPEGEQGQPGTPGTQGLNGSQGDLGPPGIIGHRGPQGPPGIEGIRGPPGPRGPQGLMGADGPQGPQGSTGDPGPMGVRGERGLEGPMGLIGWPGPDGPPGDKGRPAADAEKGDPGPKGEKGCPGSPGIKGPSGEQGHVGFVGFPGPKGQMGPIGAPGDEGQVGASGLNGSDGPKGNRGPDGPKGVTGPRGNQGRMGQRGLVGMPGASGQRGAGGPEGAEGLPGTQGPSGATGNPGSRGLTGPEGSQGDAGREGPPGQMGPPGREGIDGEPGDSGERGFTGKPGPEGPQGPVGMYGFHGPAGERGRTGYPGEKGELGPPGIQGPPGKVGLKGPPGPPGERGPPGPQGRLGEPGIQGLPGLQGPAGNPGPDGEPGKGGRPGETGPAGSIGDPGSPGYQGRDGAAGAEGPPGEKGEKGEIGPVGDQGVSGLDGEQGEPGSTGHEGSVGHKGDQGDPGPPGNPGLNGPVGDLGARGLQGLVGPPGEPGKEGDVGEIGDVGDHGPQGEKGDKGPLGSTGNPGPGGIIGERGNRGEKGDKGLSGPLGPPGLIGSTGPSGLQGEMGNVGKQGSPGLDGPQGLKGETGSTGETGQLGPPGPMGPPGKKGEKGERGQTGRPGTEGPRGINGNQGPQGFIGQEGLKGEDGSPGNTGILGVTGPRGESGPIGAPGREGKEGEKGQPGEHGVPGKPGPPGNPGQEGNVGQRGEKGDSGIDGRPGRAGNPGRKGRRGKAGPRPPRGPAGLKGLQGDKGSEGPEGPRGPYGIPGLRGTKGEPGRRGIKGELGLDGPIGLQGEEGLKGPQGPTGERGPFGIKGEQGIEGPPGSQGLPGPQGAPGPPGIEGHKGFSGSEGPRGPPGPPGLPGAPGLPGPSLNLTAQSLKRTFFHAGALSVEGPWDLLHRLEEDLKHLRSPPNGSKENPASTCKELQLVQPHLSDGHYYVDPNQGSPQDSFLVFCNFTAGGETCLLPEESQIPVRPWLQDYQASEGFQWFSTLPGGFMFRYSETGVVQLRFLRLQSSAVHQGVSYVCQGQPGVAAKDKNVKFLTDTRDQSFLVTLQGCEWEETSNRTGTLLHFAPSDLSLLPLRDLAMLHDGDIGHQFGFLVGPTCYS

Target ORF information:

RefSeq Version	XM_018090177.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090177.1

ORF Insert Sequence:

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCGAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGAT TTCCCCAACA AACACGAGTG GGAAGGTCCC CCAAGGACGG 
GGCGTCGGGA CACAACATAG GGGCAGTCTG GACTCTCAGC GGCTCATCAA CGAAGTGGAT 
CTGTTGCTTC TGGAAGAGGA AGGCTTCATT CCCTCCTTCG TCGGGGGCGA GCAGGACGGC 
GCCAAGGCGG CCAAGAAGGA GGGACAACAC AAAAAACTGG ACCCCGCTGA AGAGAACATC 
ACTATAGAGA AGCAAGAAAA GGTCCCCGAG CGCCTTCCGA CTGACAAATC CAGTAGTGAC 
CTCCTAGACT TGGCCAAAGA CCCCAAACCG ATTCCATGGT CTACACCTGC TTCCACCAGT 
GCCCCCTTCC ACTCCACGGC CGTTCCAGAT CTCTCTTTAG AAACAAAGAA CAAGACAAGT 
ATAGACATGG CACCTACTCC GTCCCATGCC ACCGATCCGC AGAAGGCTGC TGGGAACGCA 
AGCCGCGAGA GCAACGGCAT CCAGACAGGG AAACCCAAAA GACCCGTTGC CCGTGGGCCT 
CCGGGACCCC GTGGTAGTCC TGGCTGCCCT GGTCGTCCTG GATTTCCTGG ACCAAAAGGA 
GACAAGGGCC AACCGGGAAT AAGGGGGCGT ACAGGAGTGA CTGGCAACCC CGGTCCCAAC 
GGGCCCCCTG GACTACCCAC CGTAATGATC TGGAGCAACT CTAAAGAAGA CTGGGCAGCT 
TTCTATCAAA CGTCGTTCTA TCAGCTGCTG CACGCAGGCT GGCCTAGAAT CCGTGGGTCG 
ACCGGCTTCC CTGGGATGCC CGGAACGCCA GGAACTCCAG GTTCAATGGG ACCCCCGGGG 
CGACCTGGAA TCAAAGGACA GCAAGGTTCC ATGGGGACCC TGGGGCCCAA GGGTATGCAA 
GGGATGCCGG GGACACCAGG AAGGGACGGA TCTCCGGGAT TAGACGGGAA GCCGGGATCT 
CCGGGGACGA CAGGAGCCAA AGGCCCTCAA GGATTTAAAG GGGACCAAGG ACCTCTCGGC 
GAGAAAGGAG AAGAAGGATT TCAAGGATCT CCAGGGCCAA GTGGTGAGGA AGGAGTCAAA 
GGAGCCAAGG GGGTGAAGGG AGAGGCCGGT CCCGTGGGTC CTTCGGGGGT TCAGGGCCCA 
ATGGGTGCTA AAGGTTCTTT GGGATTTCCA GGCCCCCCAG GACCAATAGG AGATATGGGA 
ATTCCTGGAA CTCCTGGTCC ACCGGGGCCT GAGGGAGAAC AAGGACAGCC AGGAACCCCA 
GGAACCCAGG GCTTGAATGG GTCCCAGGGT GATCTGGGTC CTCCGGGAAT AATTGGTCAT 
CGGGGACCAC AGGGTCCTCC TGGAATAGAA GGTATAAGAG GACCTCCTGG GCCCAGAGGA 
CCACAGGGAT TGATGGGAGC CGATGGACCC CAAGGTCCAC AAGGAAGCAC AGGGGACCCT 
GGGCCAATGG GAGTAAGAGG AGAACGGGGA CTTGAAGGAC CCATGGGATT GATTGGATGG 
CCAGGACCAG ATGGACCTCC TGGTGATAAG GGTCGCCCTG CTGCAGATGC CGAAAAAGGA 
GATCCTGGAC CAAAGGGGGA AAAAGGTTGT CCAGGATCTC CTGGCATCAA AGGTCCTTCT 
GGAGAGCAAG GCCATGTTGG TTTTGTGGGT TTCCCAGGAC CTAAAGGTCA GATGGGCCCC 
ATCGGAGCTC CAGGGGATGA AGGCCAGGTT GGAGCTTCAG GTCTCAACGG CAGCGACGGA 
CCAAAGGGAA ACCGGGGACC TGATGGGCCA AAGGGGGTAA CAGGACCAAG AGGAAATCAG 
GGTCGTATGG GCCAGAGAGG ACTTGTAGGA ATGCCAGGAG CATCAGGTCA GCGAGGAGCC 
GGGGGCCCCG AGGGAGCAGA AGGACTTCCT GGTACACAGG GACCCAGTGG CGCAACAGGG 
AATCCTGGGA GTAGGGGGCT TACGGGTCCT GAAGGGTCTC AAGGGGACGC TGGAAGAGAA 
GGACCTCCAG GCCAAATGGG GCCTCCTGGG AGAGAAGGGA TTGATGGAGA ACCAGGAGAT 
TCTGGCGAAA GGGGATTTAC AGGGAAACCA GGCCCAGAAG GGCCACAGGG GCCAGTAGGC 
ATGTATGGGT TCCATGGCCC TGCTGGAGAG AGAGGTCGGA CGGGATACCC CGGGGAGAAG 
GGGGAATTGG GACCTCCTGG GATCCAAGGG CCACCAGGAA AAGTGGGACT AAAGGGTCCT 
CCTGGGCCAC CAGGGGAAAG AGGCCCACCA GGACCCCAGG GTCGACTAGG AGAACCAGGC 
ATCCAGGGTT TGCCCGGCCT TCAGGGCCCA GCTGGTAACC CGGGGCCAGA TGGGGAGCCT 
GGAAAGGGTG GACGTCCAGG TGAAACCGGG CCTGCTGGGA GTATAGGAGA CCCAGGATCT 
CCAGGATACC AGGGTCGAGA CGGAGCAGCA GGTGCTGAAG GACCCCCAGG AGAGAAAGGA 
GAAAAGGGAG AAATTGGGCC TGTAGGAGAC CAAGGGGTGA GCGGTTTGGA CGGGGAGCAG 
GGAGAACCTG GGTCTACTGG ACATGAAGGA TCAGTTGGCC ATAAAGGAGA TCAGGGGGAC 
CCTGGTCCTC CAGGGAATCC TGGTCTAAAC GGGCCTGTAG GAGACCTAGG GGCAAGAGGA 
CTGCAGGGCC TGGTGGGACC CCCAGGGGAA CCAGGGAAGG AGGGAGATGT GGGGGAAATT 
GGAGATGTTG GAGACCACGG TCCTCAAGGA GAAAAGGGAG ACAAAGGGCC ACTGGGTAGT 
ACCGGGAACC CAGGACCTGG CGGCATTATA GGAGAACGAG GCAATCGAGG TGAAAAGGGG 
GACAAAGGCC TGAGTGGACC TCTGGGACCC CCGGGGTTGA TTGGATCTAC AGGACCTTCT 
GGTCTACAGG GAGAAATGGG AAATGTTGGC AAACAAGGGT CCCCAGGTCT TGATGGACCT 
CAAGGTTTGA AGGGGGAAAC TGGTTCCACT GGAGAGACTG GTCAACTCGG TCCCCCTGGG 
CCAATGGGCC CACCTGGAAA GAAAGGGGAG AAGGGGGAGC GAGGACAGAC TGGGAGACCA 
GGAACAGAGG GGCCACGAGG CATTAACGGC AACCAGGGGC CACAAGGTTT TATTGGCCAA 
GAGGGACTCA AGGGGGAAGA TGGGAGCCCG GGAAATACAG GAATCCTGGG GGTTACGGGG 
CCACGTGGGG AGTCTGGACC CATAGGGGCA CCAGGAAGAG AAGGGAAGGA AGGAGAAAAG 
GGGCAACCAG GAGAACATGG AGTTCCAGGG AAACCGGGGC CCCCTGGAAA CCCAGGACAA 
GAAGGGAATG TGGGGCAGAG GGGAGAAAAG GGTGACAGTG GCATTGATGG CCGCCCTGGC 
AGAGCAGGGA ATCCTGGGAG AAAGGGCAGA AGAGGGAAAG CAGGTCCAAG GCCACCAAGA 
GGACCTGCAG GTCTCAAGGG TCTACAAGGA GATAAGGGTT CGGAAGGACC AGAAGGACCC 
AGAGGACCAT ATGGCATTCC TGGACTCAGA GGAACGAAAG GTGAACCGGG TCGAAGAGGT 
ATAAAGGGTG AGCTGGGCTT GGATGGACCA ATAGGACTGC AAGGAGAAGA AGGGCTGAAG 
GGACCACAAG GACCGACCGG GGAGCGGGGA CCTTTCGGGA TCAAAGGGGA ACAGGGAATT 
GAGGGGCCAC CTGGCTCTCA AGGATTACCT GGACCCCAAG GTGCACCAGG ACCACCAGGG 
ATAGAGGGAC ATAAAGGATT CTCTGGATCT GAAGGGCCCA GAGGGCCACC TGGACCACCG 
GGGCTTCCTG GTGCTCCTGG ACTCCCAGGA CCTTCTCTAA ACTTAACGGC CCAAAGTTTA 
AAGCGGACCT TCTTCCATGC CGGGGCCTTG AGCGTAGAGG GACCTTGGGA TCTTCTGCAT 
CGTTTGGAAG AGGACTTGAA ACACTTGCGC TCTCCGCCCA ATGGTTCCAA AGAAAACCCA 
GCCAGCACCT GTAAGGAACT TCAGCTGGTG CAACCTCATC TGTCGGATGG TCATTACTAT 
GTAGATCCCA ACCAGGGGAG TCCACAGGAT TCTTTCCTTG TGTTCTGTAA CTTCACTGCA 
GGCGGTGAGA CGTGTCTGCT CCCTGAGGAG AGCCAAATAC CTGTCAGACC TTGGCTTCAG 
GACTATCAGG CTTCTGAGGG TTTCCAGTGG TTCAGCACTC TTCCTGGGGG CTTTATGTTC 
AGGTACAGTG AAACAGGAGT CGTCCAGCTG CGGTTCCTGA GGCTACAGAG CAGCGCCGTA 
CATCAGGGGG TGAGCTACGT GTGCCAAGGT CAGCCCGGCG TCGCTGCGAA AGACAAGAAT 
GTGAAGTTTC TGACCGATAC CCGGGATCAG AGCTTTCTAG TGACTCTGCA GGGCTGTGAG 
TGGGAAGAGA CTTCAAACAG AACAGGAACT CTCCTCCACT TTGCCCCCTC AGACCTCAGC 
CTCCTTCCTC TCAGAGACTT GGCCATGTTA CACGATGGGG ACATTGGGCA CCAGTTTGGT 
TTCTTGGTGG GTCCTACCTG CTACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495290 ( XM_018090175.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945664.1 Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X1, mRNA.

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CloneID	OXa64301
Clone ID Related Accession (Same CDS sequence)	XM_018090175.2
Accession Version	XM_018090175.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(I) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018090175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCCAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGTT GGATGTGGGC GGAGACTGGA CTACAGACCA TGGCAAAAAC 
TACACCCGTC AATCAACCAT ACTTGAGATT TCCCCAACAA ACACGAGTGG GAAGGTCCCC 
CAAGGACGGG GCGTCGGGAC ACAACATAGG GGCAGTCTGG ACTCTCAGCG GCTCATCAAC 
GAAGTGGATC TGTTGCTTCT GGAAGAGGAA GGCTTCATTC CCTCCTTCGT CGGGGGCGAG 
CAGGACGGCG CCAAGGCGGC CAAGAAGGAG GGACAACACA AAAAACTGGA CCCCGCTGAA 
GAGAACATCA CTATAGAGAA GCAAGAAAAG GTCCCCGAGC GCCTTCCGAC TGACAAATCC 
AGTAGTGACC TCCTAGACTT GGCCAAAGAC CCCAAACCGA TTCCATGGTC TACACCTGCT 
TCCACCAGTG CCCCCTTCCA CTCCACGGCC GTTCCAGATC TCTCTTTAGA AACAAAGAAC 
AAGACAAGTA TAGACATGGC ACCTACTCCG TCCCATGCCA CCGATCCGCA GAAGGCTGCT 
GGGAACGCAA GCCGCGAGAG CAACGGCATC CAGACAGGGA AACCCAAAAG ACCCGTTGCC 
CGTGGGCCTC CGGGACCCCG TGGTAGTCCT GGCTGCCCTG GTCGTCCTGG ATTTCCTGGA 
CCAAAAGGAG ACAAGGGCCA ACCGGGAATA AGGGGGCGTA CAGGAGTGAC TGGCAACCCC 
GGTCCCAACG GGCCCCCTGG ACTACCCACC GTAATGATCT GGAGCAACTC TAAAGAAGAC 
TGGGCAGCTT TCTATCAAAC GTCGTTCTAT CAGCTGCTGC ACGCAGGCTG GCCTAGAATC 
CGTGGGTCGA CCGGCTTCCC TGGGATGCCC GGAACGCCAG GAACTCCAGG TTCAATGGGA 
CCCCCGGGGC GACCTGGAAT CAAAGGACAG CAAGGTTCCA TGGGGACCCT GGGGCCCAAG 
GGTATGCAAG GGATGCCGGG GACACCAGGA AGGGACGGAT CTCCGGGATT AGACGGGAAG 
CCGGGATCTC CGGGGACGAC AGGAGCCAAA GGCCCTCAAG GATTTAAAGG GGACCAAGGA 
CCTCTCGGCG AGAAAGGAGA AGAAGGATTT CAAGGATCTC CAGGGCCAAG TGGTGAGGAA 
GGAGTCAAAG GAGCCAAGGG GGTGAAGGGA GAGGCCGGTC CCGTGGGTCC TTCGGGGGTT 
CAGGGCCCAA TGGGTGCTAA AGGTTCTTTG GGATTTCCAG GCCCCCCAGG ACCAATAGGA 
GATATGGGAA TTCCTGGAAC TCCTGGTCCA CCGGGGCCTG AGGGAGAACA AGGACAGCCA 
GGAACCCCAG GAACCCAGGG CTTGAATGGG TCCCAGGGTG ATCTGGGTCC TCCGGGAATA 
ATTGGTCATC GGGGACCACA GGGTCCTCCT GGAATAGAAG GTATAAGAGG ACCTCCTGGG 
CCCAGAGGAC CACAGGGATT GATGGGAGCC GATGGACCCC AAGGTCCACA AGGAAGCACA 
GGGGACCCTG GGCCAATGGG AGTAAGAGGA GAACGGGGAC TTGAAGGACC CATGGGATTG 
ATTGGATGGC CAGGACCAGA TGGACCTCCT GGTGATAAGG GTCGCCCTGC TGCAGATGCC 
GAAAAAGGAG ATCCTGGACC AAAGGGGGAA AAAGGTTGTC CAGGATCTCC TGGCATCAAA 
GGTCCTTCTG GAGAGCAAGG CCATGTTGGT TTTGTGGGTT TCCCAGGACC TAAAGGTCAG 
ATGGGCCCCA TCGGAGCTCC AGGGGATGAA GGCCAGGTTG GAGCTTCAGG TCTCAACGGC 
AGCGACGGAC CAAAGGGAAA CCGGGGACCT GATGGGCCAA AGGGGGTAAC AGGACCAAGA 
GGAAATCAGG GTCGTATGGG CCAGAGAGGA CTTGTAGGAA TGCCAGGAGC ATCAGGTCAG 
CGAGGAGCCG GGGGCCCCGA GGGAGCAGAA GGACTTCCTG GTACACAGGG ACCCAGTGGC 
GCAACAGGGA ATCCTGGGAG TAGGGGGCTT ACGGGTCCTG AAGGGTCTCA AGGGGACGCT 
GGAAGAGAAG GACCTCCAGG CCAAATGGGG CCTCCTGGGA GAGAAGGGAT TGATGGAGAA 
CCAGGAGATT CTGGCGAAAG GGGATTTACA GGGAAACCAG GCCCAGAAGG GCCACAGGGG 
CCAGTAGGCA TGTATGGGTT CCATGGCCCT GCTGGAGAGA GAGGTCGGAC GGGATACCCC 
GGGGAGAAGG GGGAATTGGG ACCTCCTGGG ATCCAAGGGC CACCAGGAAA AGTGGGACTA 
AAGGGTCCTC CTGGGCCACC AGGGGAAAGA GGCCCACCAG GACCCCAGGG TCGACTAGGA 
GAACCAGGCA TCCAGGGTTT GCCCGGCCTT CAGGGCCCAG CTGGTAACCC GGGGCCAGAT 
GGGGAGCCTG GAAAGGGTGG ACGTCCAGGT GAAACCGGGC CTGCTGGGAG TATAGGAGAC 
CCAGGATCTC CAGGATACCA GGGTCGAGAC GGAGCAGCAG GTGCTGAAGG ACCCCCAGGA 
GAGAAAGGAG AAAAGGGAGA AATTGGGCCT GTAGGAGACC AAGGGGTGAG CGGTTTGGAC 
GGGGAGCAGG GAGAACCTGG GTCTACTGGA CATGAAGGAT CAGTTGGCCA TAAAGGAGAT 
CAGGGGGACC CTGGTCCTCC AGGGAATCCT GGTCTAAACG GGCCTGTAGG AGACCTAGGG 
GCAAGAGGAC TGCAGGGCCT GGTGGGACCC CCAGGGGAAC CAGGGAAGGA GGGAGATGTG 
GGGGAAATTG GAGATGTTGG AGACCACGGT CCTCAAGGAG AAAAGGGAGA CAAAGGGCCA 
CTGGGTAGTA CCGGGAACCC AGGACCTGGC GGCATTATAG GAGAACGAGG CAATCGAGGT 
GAAAAGGGGG ACAAAGGCCT GAGTGGACCT CTGGGACCCC CGGGGTTGAT TGGATCTACA 
GGACCTTCTG GTCTACAGGG AGAAATGGGA AATGTTGGCA AACAAGGGTC CCCAGGTCTT 
GATGGACCTC AAGGTTTGAA GGGGGAAACT GGTTCCACTG GAGAGACTGG TCAACTCGGT 
CCCCCTGGGC CAATGGGCCC ACCTGGAAAG AAAGGGGAGA AGGGGGAGCG AGGACAGACT 
GGGAGACCAG GAACAGAGGG GCCACGAGGC ATTAACGGCA ACCAGGGGCC ACAAGGTTTT 
ATTGGCCAAG AGGGACTCAA GGGGGAAGAT GGGAGCCCGG GAAATACAGG AATCCTGGGG 
GTTACGGGGC CACGTGGGGA GTCTGGACCC ATAGGGGCAC CAGGAAGAGA AGGGAAGGAA 
GGAGAAAAGG GGCAACCAGG AGAACATGGA GTTCCAGGGA AACCGGGGCC CCCTGGAAAC 
CCAGGACAAG AAGGGAATGT GGGGCAGAGG GGAGAAAAGG GTGACAGTGG CATTGATGGC 
CGCCCTGGCA GAGCAGGGAA TCCTGGGAGA AAGGGCAGAA GAGGGAAAGC AGGTCCAAGG 
CCACCAAGAG GACCTGCAGG TCTCAAGGGT CTACAAGGAG ATAAGGGTTC GGAAGGACCA 
GAAGGACCCA GAGGACCATA TGGCATTCCT GGACTCAGAG GAACGAAAGG TGAACCGGGT 
CGAAGAGGTA TAAAGGGTGA GCTGGGCTTG GATGGACCAA TAGGACTGCA AGGAGAAGAA 
GGGCTGAAGG GACCACAAGG ACCGACCGGG GAGCGGGGAC CTTTCGGGAT CAAAGGGGAA 
CAGGGAATTG AGGGGCCACC TGGCTCTCAA GGATTACCTG GACCCCAAGG TGCACCAGGA 
CCACCAGGGA TAGAGGGACA TAAAGGATTC TCTGGATCTG AAGGGCCCAG AGGGCCACCT 
GGACCACCGG GGCTTCCTGG TGCTCCTGGA CTCCCAGGAC CTTCTCTAAA CTTAACGGCC 
CAAAGTTTAA AGCGGACCTT CTTCCATGCC GGGGCCTTGA GCGTAGAGGG ACCTTGGGAT 
CTTCTGCATC GTTTGGAAGA GGACTTGAAA CACTTGCGCT CTCCGCCCAA TGGTTCCAAA 
GAAAACCCAG CCAGCACCTG TAAGGAACTT CAGCTGGTGC AACCTCATCT GTCGGATGGT 
CATTACTATG TAGATCCCAA CCAGGGGAGT CCACAGGATT CTTTCCTTGT GTTCTGTAAC 
TTCACTGCAG GCGGTGAGAC GTGTCTGCTC CCTGAGGAGA GCCAAATACC TGTCAGACCT 
TGGCTTCAGG ACTATCAGGC TTCTGAGGGT TTCCAGTGGT TCAGCACTCT TCCTGGGGGC 
TTTATGTTCA GGTACAGTGA AACAGGAGTC GTCCAGCTGC GGTTCCTGAG GCTACAGAGC 
AGCGCCGTAC ATCAGGGGGT GAGCTACGTG TGCCAAGGTC AGCCCGGCGT CGCTGCGAAA 
GACAAGAATG TGAAGTTTCT GACCGATACC CGGGATCAGA GCTTTCTAGT GACTCTGCAG 
GGCTGTGAGT GGGAAGAGAC TTCAAACAGA ACAGGAACTC TCCTCCACTT TGCCCCCTCA 
GACCTCAGCC TCCTTCCTCT CAGAGACTTG GCCATGTTAC ACGATGGGGA CATTGGGCAC 
CAGTTTGGTT TCTTGGTGGG TCCTACCTGC TACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945664.1
CDS	47..5425
Translation	MGRGGLSAAAIRTLGHRWALLLTLLLYRGAAGEQGDTPEAGSGQMAEEVDVLESLTLHSAELVNLTLTSEDSCLALELGQYSTLSISTMTAFGDSFADELTVLMMLRSSLREDTGLLSILSLHGLILFQIRINPKAFIFISSRRGHYEFSVPTLTDGQWHRVALSISSKGVALYVDCKLVEMLSWTNYFGLGVSTDGILMLGGLIEPYEVPFKGSLQQLVFVMGEPGAAKLYCRSYNQTCLSTFSEEHFFNLSQGDLRRREARVTKLDVGGDWTTDHGKNYTRQSTILEISPTNTSGKVPQGRGVGTQHRGSLDSQRLINEVDLLLLEEEGFIPSFVGGEQDGAKAAKKEGQHKKLDPAEENITIEKQEKVPERLPTDKSSSDLLDLAKDPKPIPWSTPASTSAPFHSTAVPDLSLETKNKTSIDMAPTPSHATDPQKAAGNASRESNGIQTGKPKRPVARGPPGPRGSPGCPGRPGFPGPKGDKGQPGIRGRTGVTGNPGPNGPPGLPTVMIWSNSKEDWAAFYQTSFYQLLHAGWPRIRGSTGFPGMPGTPGTPGSMGPPGRPGIKGQQGSMGTLGPKGMQGMPGTPGRDGSPGLDGKPGSPGTTGAKGPQGFKGDQGPLGEKGEEGFQGSPGPSGEEGVKGAKGVKGEAGPVGPSGVQGPMGAKGSLGFPGPPGPIGDMGIPGTPGPPGPEGEQGQPGTPGTQGLNGSQGDLGPPGIIGHRGPQGPPGIEGIRGPPGPRGPQGLMGADGPQGPQGSTGDPGPMGVRGERGLEGPMGLIGWPGPDGPPGDKGRPAADAEKGDPGPKGEKGCPGSPGIKGPSGEQGHVGFVGFPGPKGQMGPIGAPGDEGQVGASGLNGSDGPKGNRGPDGPKGVTGPRGNQGRMGQRGLVGMPGASGQRGAGGPEGAEGLPGTQGPSGATGNPGSRGLTGPEGSQGDAGREGPPGQMGPPGREGIDGEPGDSGERGFTGKPGPEGPQGPVGMYGFHGPAGERGRTGYPGEKGELGPPGIQGPPGKVGLKGPPGPPGERGPPGPQGRLGEPGIQGLPGLQGPAGNPGPDGEPGKGGRPGETGPAGSIGDPGSPGYQGRDGAAGAEGPPGEKGEKGEIGPVGDQGVSGLDGEQGEPGSTGHEGSVGHKGDQGDPGPPGNPGLNGPVGDLGARGLQGLVGPPGEPGKEGDVGEIGDVGDHGPQGEKGDKGPLGSTGNPGPGGIIGERGNRGEKGDKGLSGPLGPPGLIGSTGPSGLQGEMGNVGKQGSPGLDGPQGLKGETGSTGETGQLGPPGPMGPPGKKGEKGERGQTGRPGTEGPRGINGNQGPQGFIGQEGLKGEDGSPGNTGILGVTGPRGESGPIGAPGREGKEGEKGQPGEHGVPGKPGPPGNPGQEGNVGQRGEKGDSGIDGRPGRAGNPGRKGRRGKAGPRPPRGPAGLKGLQGDKGSEGPEGPRGPYGIPGLRGTKGEPGRRGIKGELGLDGPIGLQGEEGLKGPQGPTGERGPFGIKGEQGIEGPPGSQGLPGPQGAPGPPGIEGHKGFSGSEGPRGPPGPPGLPGAPGLPGPSLNLTAQSLKRTFFHAGALSVEGPWDLLHRLEEDLKHLRSPPNGSKENPASTCKELQLVQPHLSDGHYYVDPNQGSPQDSFLVFCNFTAGGETCLLPEESQIPVRPWLQDYQASEGFQWFSTLPGGFMFRYSETGVVQLRFLRLQSSAVHQGVSYVCQGQPGVAAKDKNVKFLTDTRDQSFLVTLQGCEWEETSNRTGTLLHFAPSDLSLLPLRDLAMLHDGDIGHQFGFLVGPTCYS

Target ORF information:

RefSeq Version	XM_018090175.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090175.2

ORF Insert Sequence:

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCCAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGTT GGATGTGGGC GGAGACTGGA CTACAGACCA TGGCAAAAAC 
TACACCCGTC AATCAACCAT ACTTGAGATT TCCCCAACAA ACACGAGTGG GAAGGTCCCC 
CAAGGACGGG GCGTCGGGAC ACAACATAGG GGCAGTCTGG ACTCTCAGCG GCTCATCAAC 
GAAGTGGATC TGTTGCTTCT GGAAGAGGAA GGCTTCATTC CCTCCTTCGT CGGGGGCGAG 
CAGGACGGCG CCAAGGCGGC CAAGAAGGAG GGACAACACA AAAAACTGGA CCCCGCTGAA 
GAGAACATCA CTATAGAGAA GCAAGAAAAG GTCCCCGAGC GCCTTCCGAC TGACAAATCC 
AGTAGTGACC TCCTAGACTT GGCCAAAGAC CCCAAACCGA TTCCATGGTC TACACCTGCT 
TCCACCAGTG CCCCCTTCCA CTCCACGGCC GTTCCAGATC TCTCTTTAGA AACAAAGAAC 
AAGACAAGTA TAGACATGGC ACCTACTCCG TCCCATGCCA CCGATCCGCA GAAGGCTGCT 
GGGAACGCAA GCCGCGAGAG CAACGGCATC CAGACAGGGA AACCCAAAAG ACCCGTTGCC 
CGTGGGCCTC CGGGACCCCG TGGTAGTCCT GGCTGCCCTG GTCGTCCTGG ATTTCCTGGA 
CCAAAAGGAG ACAAGGGCCA ACCGGGAATA AGGGGGCGTA CAGGAGTGAC TGGCAACCCC 
GGTCCCAACG GGCCCCCTGG ACTACCCACC GTAATGATCT GGAGCAACTC TAAAGAAGAC 
TGGGCAGCTT TCTATCAAAC GTCGTTCTAT CAGCTGCTGC ACGCAGGCTG GCCTAGAATC 
CGTGGGTCGA CCGGCTTCCC TGGGATGCCC GGAACGCCAG GAACTCCAGG TTCAATGGGA 
CCCCCGGGGC GACCTGGAAT CAAAGGACAG CAAGGTTCCA TGGGGACCCT GGGGCCCAAG 
GGTATGCAAG GGATGCCGGG GACACCAGGA AGGGACGGAT CTCCGGGATT AGACGGGAAG 
CCGGGATCTC CGGGGACGAC AGGAGCCAAA GGCCCTCAAG GATTTAAAGG GGACCAAGGA 
CCTCTCGGCG AGAAAGGAGA AGAAGGATTT CAAGGATCTC CAGGGCCAAG TGGTGAGGAA 
GGAGTCAAAG GAGCCAAGGG GGTGAAGGGA GAGGCCGGTC CCGTGGGTCC TTCGGGGGTT 
CAGGGCCCAA TGGGTGCTAA AGGTTCTTTG GGATTTCCAG GCCCCCCAGG ACCAATAGGA 
GATATGGGAA TTCCTGGAAC TCCTGGTCCA CCGGGGCCTG AGGGAGAACA AGGACAGCCA 
GGAACCCCAG GAACCCAGGG CTTGAATGGG TCCCAGGGTG ATCTGGGTCC TCCGGGAATA 
ATTGGTCATC GGGGACCACA GGGTCCTCCT GGAATAGAAG GTATAAGAGG ACCTCCTGGG 
CCCAGAGGAC CACAGGGATT GATGGGAGCC GATGGACCCC AAGGTCCACA AGGAAGCACA 
GGGGACCCTG GGCCAATGGG AGTAAGAGGA GAACGGGGAC TTGAAGGACC CATGGGATTG 
ATTGGATGGC CAGGACCAGA TGGACCTCCT GGTGATAAGG GTCGCCCTGC TGCAGATGCC 
GAAAAAGGAG ATCCTGGACC AAAGGGGGAA AAAGGTTGTC CAGGATCTCC TGGCATCAAA 
GGTCCTTCTG GAGAGCAAGG CCATGTTGGT TTTGTGGGTT TCCCAGGACC TAAAGGTCAG 
ATGGGCCCCA TCGGAGCTCC AGGGGATGAA GGCCAGGTTG GAGCTTCAGG TCTCAACGGC 
AGCGACGGAC CAAAGGGAAA CCGGGGACCT GATGGGCCAA AGGGGGTAAC AGGACCAAGA 
GGAAATCAGG GTCGTATGGG CCAGAGAGGA CTTGTAGGAA TGCCAGGAGC ATCAGGTCAG 
CGAGGAGCCG GGGGCCCCGA GGGAGCAGAA GGACTTCCTG GTACACAGGG ACCCAGTGGC 
GCAACAGGGA ATCCTGGGAG TAGGGGGCTT ACGGGTCCTG AAGGGTCTCA AGGGGACGCT 
GGAAGAGAAG GACCTCCAGG CCAAATGGGG CCTCCTGGGA GAGAAGGGAT TGATGGAGAA 
CCAGGAGATT CTGGCGAAAG GGGATTTACA GGGAAACCAG GCCCAGAAGG GCCACAGGGG 
CCAGTAGGCA TGTATGGGTT CCATGGCCCT GCTGGAGAGA GAGGTCGGAC GGGATACCCC 
GGGGAGAAGG GGGAATTGGG ACCTCCTGGG ATCCAAGGGC CACCAGGAAA AGTGGGACTA 
AAGGGTCCTC CTGGGCCACC AGGGGAAAGA GGCCCACCAG GACCCCAGGG TCGACTAGGA 
GAACCAGGCA TCCAGGGTTT GCCCGGCCTT CAGGGCCCAG CTGGTAACCC GGGGCCAGAT 
GGGGAGCCTG GAAAGGGTGG ACGTCCAGGT GAAACCGGGC CTGCTGGGAG TATAGGAGAC 
CCAGGATCTC CAGGATACCA GGGTCGAGAC GGAGCAGCAG GTGCTGAAGG ACCCCCAGGA 
GAGAAAGGAG AAAAGGGAGA AATTGGGCCT GTAGGAGACC AAGGGGTGAG CGGTTTGGAC 
GGGGAGCAGG GAGAACCTGG GTCTACTGGA CATGAAGGAT CAGTTGGCCA TAAAGGAGAT 
CAGGGGGACC CTGGTCCTCC AGGGAATCCT GGTCTAAACG GGCCTGTAGG AGACCTAGGG 
GCAAGAGGAC TGCAGGGCCT GGTGGGACCC CCAGGGGAAC CAGGGAAGGA GGGAGATGTG 
GGGGAAATTG GAGATGTTGG AGACCACGGT CCTCAAGGAG AAAAGGGAGA CAAAGGGCCA 
CTGGGTAGTA CCGGGAACCC AGGACCTGGC GGCATTATAG GAGAACGAGG CAATCGAGGT 
GAAAAGGGGG ACAAAGGCCT GAGTGGACCT CTGGGACCCC CGGGGTTGAT TGGATCTACA 
GGACCTTCTG GTCTACAGGG AGAAATGGGA AATGTTGGCA AACAAGGGTC CCCAGGTCTT 
GATGGACCTC AAGGTTTGAA GGGGGAAACT GGTTCCACTG GAGAGACTGG TCAACTCGGT 
CCCCCTGGGC CAATGGGCCC ACCTGGAAAG AAAGGGGAGA AGGGGGAGCG AGGACAGACT 
GGGAGACCAG GAACAGAGGG GCCACGAGGC ATTAACGGCA ACCAGGGGCC ACAAGGTTTT 
ATTGGCCAAG AGGGACTCAA GGGGGAAGAT GGGAGCCCGG GAAATACAGG AATCCTGGGG 
GTTACGGGGC CACGTGGGGA GTCTGGACCC ATAGGGGCAC CAGGAAGAGA AGGGAAGGAA 
GGAGAAAAGG GGCAACCAGG AGAACATGGA GTTCCAGGGA AACCGGGGCC CCCTGGAAAC 
CCAGGACAAG AAGGGAATGT GGGGCAGAGG GGAGAAAAGG GTGACAGTGG CATTGATGGC 
CGCCCTGGCA GAGCAGGGAA TCCTGGGAGA AAGGGCAGAA GAGGGAAAGC AGGTCCAAGG 
CCACCAAGAG GACCTGCAGG TCTCAAGGGT CTACAAGGAG ATAAGGGTTC GGAAGGACCA 
GAAGGACCCA GAGGACCATA TGGCATTCCT GGACTCAGAG GAACGAAAGG TGAACCGGGT 
CGAAGAGGTA TAAAGGGTGA GCTGGGCTTG GATGGACCAA TAGGACTGCA AGGAGAAGAA 
GGGCTGAAGG GACCACAAGG ACCGACCGGG GAGCGGGGAC CTTTCGGGAT CAAAGGGGAA 
CAGGGAATTG AGGGGCCACC TGGCTCTCAA GGATTACCTG GACCCCAAGG TGCACCAGGA 
CCACCAGGGA TAGAGGGACA TAAAGGATTC TCTGGATCTG AAGGGCCCAG AGGGCCACCT 
GGACCACCGG GGCTTCCTGG TGCTCCTGGA CTCCCAGGAC CTTCTCTAAA CTTAACGGCC 
CAAAGTTTAA AGCGGACCTT CTTCCATGCC GGGGCCTTGA GCGTAGAGGG ACCTTGGGAT 
CTTCTGCATC GTTTGGAAGA GGACTTGAAA CACTTGCGCT CTCCGCCCAA TGGTTCCAAA 
GAAAACCCAG CCAGCACCTG TAAGGAACTT CAGCTGGTGC AACCTCATCT GTCGGATGGT 
CATTACTATG TAGATCCCAA CCAGGGGAGT CCACAGGATT CTTTCCTTGT GTTCTGTAAC 
TTCACTGCAG GCGGTGAGAC GTGTCTGCTC CCTGAGGAGA GCCAAATACC TGTCAGACCT 
TGGCTTCAGG ACTATCAGGC TTCTGAGGGT TTCCAGTGGT TCAGCACTCT TCCTGGGGGC 
TTTATGTTCA GGTACAGTGA AACAGGAGTC GTCCAGCTGC GGTTCCTGAG GCTACAGAGC 
AGCGCCGTAC ATCAGGGGGT GAGCTACGTG TGCCAAGGTC AGCCCGGCGT CGCTGCGAAA 
GACAAGAATG TGAAGTTTCT GACCGATACC CGGGATCAGA GCTTTCTAGT GACTCTGCAG 
GGCTGTGAGT GGGAAGAGAC TTCAAACAGA ACAGGAACTC TCCTCCACTT TGCCCCCTCA 
GACCTCAGCC TCCTTCCTCT CAGAGACTTG GCCATGTTAC ACGATGGGGA CATTGGGCAC 
CAGTTTGGTT TCTTGGTGGG TCCTACCTGC TACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495290 ( XM_031891045.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746905.1 Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X2, mRNA.

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CloneID	OXa64302
Clone ID Related Accession (Same CDS sequence)	XM_031891045.1
Accession Version	XM_031891045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(I) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCCAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGTT GGATGTGGGC GGAGACTGGA CTACAGACCA TGGCAAAAAC 
TACACCCGTC AATCAACCAT ACTTGAGATT TCCCCAACAA ACACGAGTGG GAAGGTCCCC 
CAAGGACGGG GCGTCGGGAC ACAACATAGG GGCAGTCTGG ACTCTCAGCG GCTCATCAAC 
GAAGTGGATC TGTTGCTTCT GGAAGAGGAA GGCTTCATTC CCTCCTTCGT CGGGGGCGAG 
CAGGACGGCG CCAAGGCGGC CAAGAAGGAG GGACAACACA AAAAACTGGA CCCCGCTGAA 
GAGAACATCA CTATAGAGAA GCAAGAAAAG GTCCCCGAGC GCCTTCCGAC TGACAAATCC 
AGTAGTGACC TCCTAGACTT GGCCAAAGAC CCCAAACCGA TTCCATGGTC TACACCTGCT 
TCCACCAGTG CCCCCTTCCA CTCCACGGCC GTTCCAGATC TCTCTTTAGA AACAAAGAAC 
AAGACAAGTA TAGACATGGC ACCTACTCCG TCCCATGCCA CCGATCCGCA GAAGGCTGCT 
GGGAACGCAA GCCGCGAGAG CAACGGCATC CAGACAGGGA AACCCAAAAG ACCCGTTGCC 
CGTGGGCCTC CGGGACCCCG TGGTAGTCCT GGCTGCCCTG GTCGTCCTGG ATTTCCTGGA 
CCAAAAGGAG ACAAGGGCCA ACCGGGAATA AGGGGGCGTA CAGGAGTGAC TGGCAACCCC 
GGTCCCAACG GGCCCCCTGG ACTACCCACC GTAATGATCT GGAGCAACTC TAAAGAAGAC 
TGGGCAGCTT TCTATCAAAC GTCGTTCTAT CAGCTGCTGC ACGCAGGCTG GCCTAGAATC 
CGTGGGTCGA CCGGCTTCCC TGGGATGCCC GGAACGCCAG GAACTCCAGG TTCAATGGGA 
CCCCCGGGGC GACCTGGAAT CAAAGGACAG CAAGGTTCCA TGGGGACCCT GGGGCCCAAG 
GGTATGCAAG GGATGCCGGG GACACCAGGA AGGGACGGAT CTCCGGGATT AGACGGGAAG 
CCGGGATCTC CGGGGACGAC AGGAGCCAAA GGCCCTCAAG GATTTAAAGG GGACCAAGGA 
CCTCTCGGCG AGAAAGGAGA AGAAGGATTT CAAGGATCTC CAGGGCCAAG TGGTGAGGAA 
GGAGTCAAAG GAGCCAAGGG GGTGAAGGGA GAGGCCGGTC CCGTGGGTCC TTCGGGGGTT 
CAGGGCCCAA TGGGTGCTAA AGGTTCTTTG GGATTTCCAG GCCCCCCAGG ACCAATAGGA 
GATATGGGAA TTCCTGGAAC TCCTGGTCCA CCGGGGCCTG AGGGAGAACA AGGACAGCCA 
GGAACCCCAG GAACCCAGGG CTTGAATGGG TCCCAGGGTG ATCTGGGTCC TCCGGGAATA 
ATTGGTCATC GGGGACCACA GGGTCCTCCT GGAATAGAAG GTATAAGAGG ACCTCCTGGG 
CCCAGAGGAC CACAGGGATT GATGGGAGCC GATGGACCCC AAGGTCCACA AGGAAGCACA 
GGGGACCCTG GGCCAATGGG AGTAAGAGGA GAACGGGGAC TTGAAGGACC CATGGGATTG 
ATTGGATGGC CAGGACCAGA TGGACCTCCT GGTGATAAGG GTCGCCCTGC TGCAGATGCC 
GAAAAAGGAG ATCCTGGACC AAAGGGGGAA AAAGGTTGTC CAGGATCTCC TGGCATCAAA 
GGTCCTTCTG GAGAGCAAGG CCATGTTGGT TTTGTGGGTT TCCCAGGACC TAAAGGTCAG 
ATGGGCCCCA TCGGAGCTCC AGGGGATGAA GGCCAGGTTG GAGCTTCAGG TCTCAACGGC 
AGCGACGGAC CAAAGGGAAA CCGGGGACCT GATGGGCCAA AGGGGGTAAC AGGACCAAGA 
GGAAATCAGG GTCGTATGGG CCAGAGAGGA CTTGTAGGAA TGCCAGGAGC ATCAGGTCAG 
CGAGGAGCCG GGGGCCCCGA GGGAGCAGAA GGACTTCCTG GTACACAGGG ACCCAGTGGC 
GCAACAGGGA ATCCTGGGAG TAGGGGGCTT ACGGGTCCTG AAGGGTCTCA AGGGGACGCT 
GGAAGAGAAG GACCTCCAGG CCAAATGGGG CCTCCTGGGA GAGAAGGGAT TGATGGAGAA 
CCAGGAGATT CTGGCGAAAG GGGATTTACA GGGAAACCAG GCCCAGAAGG GCCACAGGGG 
CCAGTAGGCA TGTATGGGTT CCATGGCCCT GCTGGAGAGA GAGGTCGGAC GGGATACCCC 
GGGGAGAAGG GGGAATTGGG ACCTCCTGGG ATCCAAGGGC CACCAGGAAA AGTGGGACTA 
AAGGGTCCTC CTGGGCCACC AGGGGAAAGA GGCCCACCAG GACCCCAGGG TCGACTAGGA 
GAACCAGGCA TCCAGGGTTT GCCCGGCCTT CAGGGCCCAG CTGGTAACCC GGGGCCAGAT 
GGGGAGCCTG GAAAGGGTGG ACGTCCAGGT GAAACCGGGC CTGCTGGGAG TATAGGAGAC 
CCAGGATCTC CAGGATACCA GGGTCGAGAC GGAGCAGCAG GTGCTGAAGG ACCCCCAGGA 
GAGAAAGGAG AAAAGGGAGA AATTGGGCCT GTAGGAGACC AAGGGGTGAG CGGTTTGGAC 
GGGGAGCAGG GAGAACCTGG GTCTACTGGA CATGAAGGAT CAGTTGGCCA TAAAGGAGAT 
CAGGGGGACC CTGGTCCTCC AGGGAATCCT GGTCTAAACG GGCCTGTAGG AGACCTAGGG 
GCAAGAGGAC TGCAGGGCCT GGTGGGACCC CCAGGGGAAC CAGGGAAGGA GGGAGATGTG 
GGGGAAATTG GAGATGTTGG AGACCACGGT CCTCAAGGAG AAAAGGGAGA CAAAGGGCCA 
CTGGGTAGTA CCGGGAACCC AGGACCTGGC GGCATTATAG GAGAACGAGG CAATCGAGGT 
GAAAAGGGGG ACAAAGGCCT GAGTGGACCT CTGGGACCCC CGGGGTTGAT TGGATCTACA 
GGACCTTCTG GTCTACAGGG AGAAATGGGA AATGTTGGCA AACAAGGGTC CCCAGGTCTT 
GATGGACCTC AAGGTTTGAA GGGGGAAACT GGTTCCACTG GAGAGACTGG TCAACTCGGT 
CCCCCTGGGC CAATGGGCCC ACCTGGAAAG AAAGGGGAGA AGGGGGAGCG AGGACAGACT 
GGGAGACCAG GAACAGAGGG GCCACGAGGC ATTAACGGCA ACCAGGGGCC ACAAGGTTTT 
ATTGGCCAAG AGGGACTCAA GGGGGAAGAT GGGAGCCCGG GAAATACAGG AATCCTGGGG 
GTTACGGGGC CACGTGGGGA GTCTGGACCC ATAGGGGCAC CAGGAAGAGA AGGGAAGGAA 
GGAGAAAAGG GGCAACCAGG AGAACATGGA GTTCCAGGGA AACCGGGGCC CCCTGGAAAC 
CCAGGACAAG AAGGGAATGT GGGGCAGAGG GGAGAAAAGG GCAGAAGAGG GAAAGCAGGT 
CCAAGGCCAC CAAGAGGACC TGCAGGTCTC AAGGGTCTAC AAGGAGATAA GGGTTCGGAA 
GGACCAGAAG GACCCAGAGG ACCATATGGC ATTCCTGGAC TCAGAGGAAC GAAAGGTGAA 
CCGGGTCGAA GAGGTATAAA GGGTGAGCTG GGCTTGGATG GACCAATAGG ACTGCAAGGA 
GAAGAAGGGC TGAAGGGACC ACAAGGACCG ACCGGGGAGC GGGGACCTTT CGGGATCAAA 
GGGGAACAGG GAATTGAGGG GCCACCTGGC TCTCAAGGAT TACCTGGACC CCAAGGTGCA 
CCAGGACCAC CAGGGATAGA GGGACATAAA GGATTCTCTG GATCTGAAGG GCCCAGAGGG 
CCACCTGGAC CACCGGGGCT TCCTGGTGCT CCTGGACTCC CAGGACCTTC TCTAAACTTA 
ACGGCCCAAA GTTTAAAGCG GACCTTCTTC CATGCCGGGG CCTTGAGCGT AGAGGGACCT 
TGGGATCTTC TGCATCGTTT GGAAGAGGAC TTGAAACACT TGCGCTCTCC GCCCAATGGT 
TCCAAAGAAA ACCCAGCCAG CACCTGTAAG GAACTTCAGC TGGTGCAACC TCATCTGTCG 
GATGGTCATT ACTATGTAGA TCCCAACCAG GGGAGTCCAC AGGATTCTTT CCTTGTGTTC 
TGTAACTTCA CTGCAGGCGG TGAGACGTGT CTGCTCCCTG AGGAGAGCCA AATACCTGTC 
AGACCTTGGC TTCAGGACTA TCAGGCTTCT GAGGGTTTCC AGTGGTTCAG CACTCTTCCT 
GGGGGCTTTA TGTTCAGGTA CAGTGAAACA GGAGTCGTCC AGCTGCGGTT CCTGAGGCTA 
CAGAGCAGCG CCGTACATCA GGGGGTGAGC TACGTGTGCC AAGGTCAGCC CGGCGTCGCT 
GCGAAAGACA AGAATGTGAA GTTTCTGACC GATACCCGGG ATCAGAGCTT TCTAGTGACT 
CTGCAGGGCT GTGAGTGGGA AGAGACTTCA AACAGAACAG GAACTCTCCT CCACTTTGCC 
CCCTCAGACC TCAGCCTCCT TCCTCTCAGA GACTTGGCCA TGTTACACGA TGGGGACATT 
GGGCACCAGT TTGGTTTCTT GGTGGGTCCT ACCTGCTACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746905.1
CDS	47..5371
Translation	MGRGGLSAAAIRTLGHRWALLLTLLLYRGAAGEQGDTPEAGSGQMAEEVDVLESLTLHSAELVNLTLTSEDSCLALELGQYSTLSISTMTAFGDSFADELTVLMMLRSSLREDTGLLSILSLHGLILFQIRINPKAFIFISSRRGHYEFSVPTLTDGQWHRVALSISSKGVALYVDCKLVEMLSWTNYFGLGVSTDGILMLGGLIEPYEVPFKGSLQQLVFVMGEPGAAKLYCRSYNQTCLSTFSEEHFFNLSQGDLRRREARVTKLDVGGDWTTDHGKNYTRQSTILEISPTNTSGKVPQGRGVGTQHRGSLDSQRLINEVDLLLLEEEGFIPSFVGGEQDGAKAAKKEGQHKKLDPAEENITIEKQEKVPERLPTDKSSSDLLDLAKDPKPIPWSTPASTSAPFHSTAVPDLSLETKNKTSIDMAPTPSHATDPQKAAGNASRESNGIQTGKPKRPVARGPPGPRGSPGCPGRPGFPGPKGDKGQPGIRGRTGVTGNPGPNGPPGLPTVMIWSNSKEDWAAFYQTSFYQLLHAGWPRIRGSTGFPGMPGTPGTPGSMGPPGRPGIKGQQGSMGTLGPKGMQGMPGTPGRDGSPGLDGKPGSPGTTGAKGPQGFKGDQGPLGEKGEEGFQGSPGPSGEEGVKGAKGVKGEAGPVGPSGVQGPMGAKGSLGFPGPPGPIGDMGIPGTPGPPGPEGEQGQPGTPGTQGLNGSQGDLGPPGIIGHRGPQGPPGIEGIRGPPGPRGPQGLMGADGPQGPQGSTGDPGPMGVRGERGLEGPMGLIGWPGPDGPPGDKGRPAADAEKGDPGPKGEKGCPGSPGIKGPSGEQGHVGFVGFPGPKGQMGPIGAPGDEGQVGASGLNGSDGPKGNRGPDGPKGVTGPRGNQGRMGQRGLVGMPGASGQRGAGGPEGAEGLPGTQGPSGATGNPGSRGLTGPEGSQGDAGREGPPGQMGPPGREGIDGEPGDSGERGFTGKPGPEGPQGPVGMYGFHGPAGERGRTGYPGEKGELGPPGIQGPPGKVGLKGPPGPPGERGPPGPQGRLGEPGIQGLPGLQGPAGNPGPDGEPGKGGRPGETGPAGSIGDPGSPGYQGRDGAAGAEGPPGEKGEKGEIGPVGDQGVSGLDGEQGEPGSTGHEGSVGHKGDQGDPGPPGNPGLNGPVGDLGARGLQGLVGPPGEPGKEGDVGEIGDVGDHGPQGEKGDKGPLGSTGNPGPGGIIGERGNRGEKGDKGLSGPLGPPGLIGSTGPSGLQGEMGNVGKQGSPGLDGPQGLKGETGSTGETGQLGPPGPMGPPGKKGEKGERGQTGRPGTEGPRGINGNQGPQGFIGQEGLKGEDGSPGNTGILGVTGPRGESGPIGAPGREGKEGEKGQPGEHGVPGKPGPPGNPGQEGNVGQRGEKGRRGKAGPRPPRGPAGLKGLQGDKGSEGPEGPRGPYGIPGLRGTKGEPGRRGIKGELGLDGPIGLQGEEGLKGPQGPTGERGPFGIKGEQGIEGPPGSQGLPGPQGAPGPPGIEGHKGFSGSEGPRGPPGPPGLPGAPGLPGPSLNLTAQSLKRTFFHAGALSVEGPWDLLHRLEEDLKHLRSPPNGSKENPASTCKELQLVQPHLSDGHYYVDPNQGSPQDSFLVFCNFTAGGETCLLPEESQIPVRPWLQDYQASEGFQWFSTLPGGFMFRYSETGVVQLRFLRLQSSAVHQGVSYVCQGQPGVAAKDKNVKFLTDTRDQSFLVTLQGCEWEETSNRTGTLLHFAPSDLSLLPLRDLAMLHDGDIGHQFGFLVGPTCYS

Target ORF information:

RefSeq Version	XM_031891045.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891045.1

ORF Insert Sequence:

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCCAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGTT GGATGTGGGC GGAGACTGGA CTACAGACCA TGGCAAAAAC 
TACACCCGTC AATCAACCAT ACTTGAGATT TCCCCAACAA ACACGAGTGG GAAGGTCCCC 
CAAGGACGGG GCGTCGGGAC ACAACATAGG GGCAGTCTGG ACTCTCAGCG GCTCATCAAC 
GAAGTGGATC TGTTGCTTCT GGAAGAGGAA GGCTTCATTC CCTCCTTCGT CGGGGGCGAG 
CAGGACGGCG CCAAGGCGGC CAAGAAGGAG GGACAACACA AAAAACTGGA CCCCGCTGAA 
GAGAACATCA CTATAGAGAA GCAAGAAAAG GTCCCCGAGC GCCTTCCGAC TGACAAATCC 
AGTAGTGACC TCCTAGACTT GGCCAAAGAC CCCAAACCGA TTCCATGGTC TACACCTGCT 
TCCACCAGTG CCCCCTTCCA CTCCACGGCC GTTCCAGATC TCTCTTTAGA AACAAAGAAC 
AAGACAAGTA TAGACATGGC ACCTACTCCG TCCCATGCCA CCGATCCGCA GAAGGCTGCT 
GGGAACGCAA GCCGCGAGAG CAACGGCATC CAGACAGGGA AACCCAAAAG ACCCGTTGCC 
CGTGGGCCTC CGGGACCCCG TGGTAGTCCT GGCTGCCCTG GTCGTCCTGG ATTTCCTGGA 
CCAAAAGGAG ACAAGGGCCA ACCGGGAATA AGGGGGCGTA CAGGAGTGAC TGGCAACCCC 
GGTCCCAACG GGCCCCCTGG ACTACCCACC GTAATGATCT GGAGCAACTC TAAAGAAGAC 
TGGGCAGCTT TCTATCAAAC GTCGTTCTAT CAGCTGCTGC ACGCAGGCTG GCCTAGAATC 
CGTGGGTCGA CCGGCTTCCC TGGGATGCCC GGAACGCCAG GAACTCCAGG TTCAATGGGA 
CCCCCGGGGC GACCTGGAAT CAAAGGACAG CAAGGTTCCA TGGGGACCCT GGGGCCCAAG 
GGTATGCAAG GGATGCCGGG GACACCAGGA AGGGACGGAT CTCCGGGATT AGACGGGAAG 
CCGGGATCTC CGGGGACGAC AGGAGCCAAA GGCCCTCAAG GATTTAAAGG GGACCAAGGA 
CCTCTCGGCG AGAAAGGAGA AGAAGGATTT CAAGGATCTC CAGGGCCAAG TGGTGAGGAA 
GGAGTCAAAG GAGCCAAGGG GGTGAAGGGA GAGGCCGGTC CCGTGGGTCC TTCGGGGGTT 
CAGGGCCCAA TGGGTGCTAA AGGTTCTTTG GGATTTCCAG GCCCCCCAGG ACCAATAGGA 
GATATGGGAA TTCCTGGAAC TCCTGGTCCA CCGGGGCCTG AGGGAGAACA AGGACAGCCA 
GGAACCCCAG GAACCCAGGG CTTGAATGGG TCCCAGGGTG ATCTGGGTCC TCCGGGAATA 
ATTGGTCATC GGGGACCACA GGGTCCTCCT GGAATAGAAG GTATAAGAGG ACCTCCTGGG 
CCCAGAGGAC CACAGGGATT GATGGGAGCC GATGGACCCC AAGGTCCACA AGGAAGCACA 
GGGGACCCTG GGCCAATGGG AGTAAGAGGA GAACGGGGAC TTGAAGGACC CATGGGATTG 
ATTGGATGGC CAGGACCAGA TGGACCTCCT GGTGATAAGG GTCGCCCTGC TGCAGATGCC 
GAAAAAGGAG ATCCTGGACC AAAGGGGGAA AAAGGTTGTC CAGGATCTCC TGGCATCAAA 
GGTCCTTCTG GAGAGCAAGG CCATGTTGGT TTTGTGGGTT TCCCAGGACC TAAAGGTCAG 
ATGGGCCCCA TCGGAGCTCC AGGGGATGAA GGCCAGGTTG GAGCTTCAGG TCTCAACGGC 
AGCGACGGAC CAAAGGGAAA CCGGGGACCT GATGGGCCAA AGGGGGTAAC AGGACCAAGA 
GGAAATCAGG GTCGTATGGG CCAGAGAGGA CTTGTAGGAA TGCCAGGAGC ATCAGGTCAG 
CGAGGAGCCG GGGGCCCCGA GGGAGCAGAA GGACTTCCTG GTACACAGGG ACCCAGTGGC 
GCAACAGGGA ATCCTGGGAG TAGGGGGCTT ACGGGTCCTG AAGGGTCTCA AGGGGACGCT 
GGAAGAGAAG GACCTCCAGG CCAAATGGGG CCTCCTGGGA GAGAAGGGAT TGATGGAGAA 
CCAGGAGATT CTGGCGAAAG GGGATTTACA GGGAAACCAG GCCCAGAAGG GCCACAGGGG 
CCAGTAGGCA TGTATGGGTT CCATGGCCCT GCTGGAGAGA GAGGTCGGAC GGGATACCCC 
GGGGAGAAGG GGGAATTGGG ACCTCCTGGG ATCCAAGGGC CACCAGGAAA AGTGGGACTA 
AAGGGTCCTC CTGGGCCACC AGGGGAAAGA GGCCCACCAG GACCCCAGGG TCGACTAGGA 
GAACCAGGCA TCCAGGGTTT GCCCGGCCTT CAGGGCCCAG CTGGTAACCC GGGGCCAGAT 
GGGGAGCCTG GAAAGGGTGG ACGTCCAGGT GAAACCGGGC CTGCTGGGAG TATAGGAGAC 
CCAGGATCTC CAGGATACCA GGGTCGAGAC GGAGCAGCAG GTGCTGAAGG ACCCCCAGGA 
GAGAAAGGAG AAAAGGGAGA AATTGGGCCT GTAGGAGACC AAGGGGTGAG CGGTTTGGAC 
GGGGAGCAGG GAGAACCTGG GTCTACTGGA CATGAAGGAT CAGTTGGCCA TAAAGGAGAT 
CAGGGGGACC CTGGTCCTCC AGGGAATCCT GGTCTAAACG GGCCTGTAGG AGACCTAGGG 
GCAAGAGGAC TGCAGGGCCT GGTGGGACCC CCAGGGGAAC CAGGGAAGGA GGGAGATGTG 
GGGGAAATTG GAGATGTTGG AGACCACGGT CCTCAAGGAG AAAAGGGAGA CAAAGGGCCA 
CTGGGTAGTA CCGGGAACCC AGGACCTGGC GGCATTATAG GAGAACGAGG CAATCGAGGT 
GAAAAGGGGG ACAAAGGCCT GAGTGGACCT CTGGGACCCC CGGGGTTGAT TGGATCTACA 
GGACCTTCTG GTCTACAGGG AGAAATGGGA AATGTTGGCA AACAAGGGTC CCCAGGTCTT 
GATGGACCTC AAGGTTTGAA GGGGGAAACT GGTTCCACTG GAGAGACTGG TCAACTCGGT 
CCCCCTGGGC CAATGGGCCC ACCTGGAAAG AAAGGGGAGA AGGGGGAGCG AGGACAGACT 
GGGAGACCAG GAACAGAGGG GCCACGAGGC ATTAACGGCA ACCAGGGGCC ACAAGGTTTT 
ATTGGCCAAG AGGGACTCAA GGGGGAAGAT GGGAGCCCGG GAAATACAGG AATCCTGGGG 
GTTACGGGGC CACGTGGGGA GTCTGGACCC ATAGGGGCAC CAGGAAGAGA AGGGAAGGAA 
GGAGAAAAGG GGCAACCAGG AGAACATGGA GTTCCAGGGA AACCGGGGCC CCCTGGAAAC 
CCAGGACAAG AAGGGAATGT GGGGCAGAGG GGAGAAAAGG GCAGAAGAGG GAAAGCAGGT 
CCAAGGCCAC CAAGAGGACC TGCAGGTCTC AAGGGTCTAC AAGGAGATAA GGGTTCGGAA 
GGACCAGAAG GACCCAGAGG ACCATATGGC ATTCCTGGAC TCAGAGGAAC GAAAGGTGAA 
CCGGGTCGAA GAGGTATAAA GGGTGAGCTG GGCTTGGATG GACCAATAGG ACTGCAAGGA 
GAAGAAGGGC TGAAGGGACC ACAAGGACCG ACCGGGGAGC GGGGACCTTT CGGGATCAAA 
GGGGAACAGG GAATTGAGGG GCCACCTGGC TCTCAAGGAT TACCTGGACC CCAAGGTGCA 
CCAGGACCAC CAGGGATAGA GGGACATAAA GGATTCTCTG GATCTGAAGG GCCCAGAGGG 
CCACCTGGAC CACCGGGGCT TCCTGGTGCT CCTGGACTCC CAGGACCTTC TCTAAACTTA 
ACGGCCCAAA GTTTAAAGCG GACCTTCTTC CATGCCGGGG CCTTGAGCGT AGAGGGACCT 
TGGGATCTTC TGCATCGTTT GGAAGAGGAC TTGAAACACT TGCGCTCTCC GCCCAATGGT 
TCCAAAGAAA ACCCAGCCAG CACCTGTAAG GAACTTCAGC TGGTGCAACC TCATCTGTCG 
GATGGTCATT ACTATGTAGA TCCCAACCAG GGGAGTCCAC AGGATTCTTT CCTTGTGTTC 
TGTAACTTCA CTGCAGGCGG TGAGACGTGT CTGCTCCCTG AGGAGAGCCA AATACCTGTC 
AGACCTTGGC TTCAGGACTA TCAGGCTTCT GAGGGTTTCC AGTGGTTCAG CACTCTTCCT 
GGGGGCTTTA TGTTCAGGTA CAGTGAAACA GGAGTCGTCC AGCTGCGGTT CCTGAGGCTA 
CAGAGCAGCG CCGTACATCA GGGGGTGAGC TACGTGTGCC AAGGTCAGCC CGGCGTCGCT 
GCGAAAGACA AGAATGTGAA GTTTCTGACC GATACCCGGG ATCAGAGCTT TCTAGTGACT 
CTGCAGGGCT GTGAGTGGGA AGAGACTTCA AACAGAACAG GAACTCTCCT CCACTTTGCC 
CCCTCAGACC TCAGCCTCCT TCCTCTCAGA GACTTGGCCA TGTTACACGA TGGGGACATT 
GGGCACCAGT TTGGTTTCTT GGTGGGTCCT ACCTGCTACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495290 ( XM_018090177.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945666.1 Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X3, mRNA.

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CloneID	OXa64303
Clone ID Related Accession (Same CDS sequence)	XM_018090177.2
Accession Version	XM_018090177.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(I) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018090177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCCAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGAT TTCCCCAACA AACACGAGTG GGAAGGTCCC CCAAGGACGG 
GGCGTCGGGA CACAACATAG GGGCAGTCTG GACTCTCAGC GGCTCATCAA CGAAGTGGAT 
CTGTTGCTTC TGGAAGAGGA AGGCTTCATT CCCTCCTTCG TCGGGGGCGA GCAGGACGGC 
GCCAAGGCGG CCAAGAAGGA GGGACAACAC AAAAAACTGG ACCCCGCTGA AGAGAACATC 
ACTATAGAGA AGCAAGAAAA GGTCCCCGAG CGCCTTCCGA CTGACAAATC CAGTAGTGAC 
CTCCTAGACT TGGCCAAAGA CCCCAAACCG ATTCCATGGT CTACACCTGC TTCCACCAGT 
GCCCCCTTCC ACTCCACGGC CGTTCCAGAT CTCTCTTTAG AAACAAAGAA CAAGACAAGT 
ATAGACATGG CACCTACTCC GTCCCATGCC ACCGATCCGC AGAAGGCTGC TGGGAACGCA 
AGCCGCGAGA GCAACGGCAT CCAGACAGGG AAACCCAAAA GACCCGTTGC CCGTGGGCCT 
CCGGGACCCC GTGGTAGTCC TGGCTGCCCT GGTCGTCCTG GATTTCCTGG ACCAAAAGGA 
GACAAGGGCC AACCGGGAAT AAGGGGGCGT ACAGGAGTGA CTGGCAACCC CGGTCCCAAC 
GGGCCCCCTG GACTACCCAC CGTAATGATC TGGAGCAACT CTAAAGAAGA CTGGGCAGCT 
TTCTATCAAA CGTCGTTCTA TCAGCTGCTG CACGCAGGCT GGCCTAGAAT CCGTGGGTCG 
ACCGGCTTCC CTGGGATGCC CGGAACGCCA GGAACTCCAG GTTCAATGGG ACCCCCGGGG 
CGACCTGGAA TCAAAGGACA GCAAGGTTCC ATGGGGACCC TGGGGCCCAA GGGTATGCAA 
GGGATGCCGG GGACACCAGG AAGGGACGGA TCTCCGGGAT TAGACGGGAA GCCGGGATCT 
CCGGGGACGA CAGGAGCCAA AGGCCCTCAA GGATTTAAAG GGGACCAAGG ACCTCTCGGC 
GAGAAAGGAG AAGAAGGATT TCAAGGATCT CCAGGGCCAA GTGGTGAGGA AGGAGTCAAA 
GGAGCCAAGG GGGTGAAGGG AGAGGCCGGT CCCGTGGGTC CTTCGGGGGT TCAGGGCCCA 
ATGGGTGCTA AAGGTTCTTT GGGATTTCCA GGCCCCCCAG GACCAATAGG AGATATGGGA 
ATTCCTGGAA CTCCTGGTCC ACCGGGGCCT GAGGGAGAAC AAGGACAGCC AGGAACCCCA 
GGAACCCAGG GCTTGAATGG GTCCCAGGGT GATCTGGGTC CTCCGGGAAT AATTGGTCAT 
CGGGGACCAC AGGGTCCTCC TGGAATAGAA GGTATAAGAG GACCTCCTGG GCCCAGAGGA 
CCACAGGGAT TGATGGGAGC CGATGGACCC CAAGGTCCAC AAGGAAGCAC AGGGGACCCT 
GGGCCAATGG GAGTAAGAGG AGAACGGGGA CTTGAAGGAC CCATGGGATT GATTGGATGG 
CCAGGACCAG ATGGACCTCC TGGTGATAAG GGTCGCCCTG CTGCAGATGC CGAAAAAGGA 
GATCCTGGAC CAAAGGGGGA AAAAGGTTGT CCAGGATCTC CTGGCATCAA AGGTCCTTCT 
GGAGAGCAAG GCCATGTTGG TTTTGTGGGT TTCCCAGGAC CTAAAGGTCA GATGGGCCCC 
ATCGGAGCTC CAGGGGATGA AGGCCAGGTT GGAGCTTCAG GTCTCAACGG CAGCGACGGA 
CCAAAGGGAA ACCGGGGACC TGATGGGCCA AAGGGGGTAA CAGGACCAAG AGGAAATCAG 
GGTCGTATGG GCCAGAGAGG ACTTGTAGGA ATGCCAGGAG CATCAGGTCA GCGAGGAGCC 
GGGGGCCCCG AGGGAGCAGA AGGACTTCCT GGTACACAGG GACCCAGTGG CGCAACAGGG 
AATCCTGGGA GTAGGGGGCT TACGGGTCCT GAAGGGTCTC AAGGGGACGC TGGAAGAGAA 
GGACCTCCAG GCCAAATGGG GCCTCCTGGG AGAGAAGGGA TTGATGGAGA ACCAGGAGAT 
TCTGGCGAAA GGGGATTTAC AGGGAAACCA GGCCCAGAAG GGCCACAGGG GCCAGTAGGC 
ATGTATGGGT TCCATGGCCC TGCTGGAGAG AGAGGTCGGA CGGGATACCC CGGGGAGAAG 
GGGGAATTGG GACCTCCTGG GATCCAAGGG CCACCAGGAA AAGTGGGACT AAAGGGTCCT 
CCTGGGCCAC CAGGGGAAAG AGGCCCACCA GGACCCCAGG GTCGACTAGG AGAACCAGGC 
ATCCAGGGTT TGCCCGGCCT TCAGGGCCCA GCTGGTAACC CGGGGCCAGA TGGGGAGCCT 
GGAAAGGGTG GACGTCCAGG TGAAACCGGG CCTGCTGGGA GTATAGGAGA CCCAGGATCT 
CCAGGATACC AGGGTCGAGA CGGAGCAGCA GGTGCTGAAG GACCCCCAGG AGAGAAAGGA 
GAAAAGGGAG AAATTGGGCC TGTAGGAGAC CAAGGGGTGA GCGGTTTGGA CGGGGAGCAG 
GGAGAACCTG GGTCTACTGG ACATGAAGGA TCAGTTGGCC ATAAAGGAGA TCAGGGGGAC 
CCTGGTCCTC CAGGGAATCC TGGTCTAAAC GGGCCTGTAG GAGACCTAGG GGCAAGAGGA 
CTGCAGGGCC TGGTGGGACC CCCAGGGGAA CCAGGGAAGG AGGGAGATGT GGGGGAAATT 
GGAGATGTTG GAGACCACGG TCCTCAAGGA GAAAAGGGAG ACAAAGGGCC ACTGGGTAGT 
ACCGGGAACC CAGGACCTGG CGGCATTATA GGAGAACGAG GCAATCGAGG TGAAAAGGGG 
GACAAAGGCC TGAGTGGACC TCTGGGACCC CCGGGGTTGA TTGGATCTAC AGGACCTTCT 
GGTCTACAGG GAGAAATGGG AAATGTTGGC AAACAAGGGT CCCCAGGTCT TGATGGACCT 
CAAGGTTTGA AGGGGGAAAC TGGTTCCACT GGAGAGACTG GTCAACTCGG TCCCCCTGGG 
CCAATGGGCC CACCTGGAAA GAAAGGGGAG AAGGGGGAGC GAGGACAGAC TGGGAGACCA 
GGAACAGAGG GGCCACGAGG CATTAACGGC AACCAGGGGC CACAAGGTTT TATTGGCCAA 
GAGGGACTCA AGGGGGAAGA TGGGAGCCCG GGAAATACAG GAATCCTGGG GGTTACGGGG 
CCACGTGGGG AGTCTGGACC CATAGGGGCA CCAGGAAGAG AAGGGAAGGA AGGAGAAAAG 
GGGCAACCAG GAGAACATGG AGTTCCAGGG AAACCGGGGC CCCCTGGAAA CCCAGGACAA 
GAAGGGAATG TGGGGCAGAG GGGAGAAAAG GGTGACAGTG GCATTGATGG CCGCCCTGGC 
AGAGCAGGGA ATCCTGGGAG AAAGGGCAGA AGAGGGAAAG CAGGTCCAAG GCCACCAAGA 
GGACCTGCAG GTCTCAAGGG TCTACAAGGA GATAAGGGTT CGGAAGGACC AGAAGGACCC 
AGAGGACCAT ATGGCATTCC TGGACTCAGA GGAACGAAAG GTGAACCGGG TCGAAGAGGT 
ATAAAGGGTG AGCTGGGCTT GGATGGACCA ATAGGACTGC AAGGAGAAGA AGGGCTGAAG 
GGACCACAAG GACCGACCGG GGAGCGGGGA CCTTTCGGGA TCAAAGGGGA ACAGGGAATT 
GAGGGGCCAC CTGGCTCTCA AGGATTACCT GGACCCCAAG GTGCACCAGG ACCACCAGGG 
ATAGAGGGAC ATAAAGGATT CTCTGGATCT GAAGGGCCCA GAGGGCCACC TGGACCACCG 
GGGCTTCCTG GTGCTCCTGG ACTCCCAGGA CCTTCTCTAA ACTTAACGGC CCAAAGTTTA 
AAGCGGACCT TCTTCCATGC CGGGGCCTTG AGCGTAGAGG GACCTTGGGA TCTTCTGCAT 
CGTTTGGAAG AGGACTTGAA ACACTTGCGC TCTCCGCCCA ATGGTTCCAA AGAAAACCCA 
GCCAGCACCT GTAAGGAACT TCAGCTGGTG CAACCTCATC TGTCGGATGG TCATTACTAT 
GTAGATCCCA ACCAGGGGAG TCCACAGGAT TCTTTCCTTG TGTTCTGTAA CTTCACTGCA 
GGCGGTGAGA CGTGTCTGCT CCCTGAGGAG AGCCAAATAC CTGTCAGACC TTGGCTTCAG 
GACTATCAGG CTTCTGAGGG TTTCCAGTGG TTCAGCACTC TTCCTGGGGG CTTTATGTTC 
AGGTACAGTG AAACAGGAGT CGTCCAGCTG CGGTTCCTGA GGCTACAGAG CAGCGCCGTA 
CATCAGGGGG TGAGCTACGT GTGCCAAGGT CAGCCCGGCG TCGCTGCGAA AGACAAGAAT 
GTGAAGTTTC TGACCGATAC CCGGGATCAG AGCTTTCTAG TGACTCTGCA GGGCTGTGAG 
TGGGAAGAGA CTTCAAACAG AACAGGAACT CTCCTCCACT TTGCCCCCTC AGACCTCAGC 
CTCCTTCCTC TCAGAGACTT GGCCATGTTA CACGATGGGG ACATTGGGCA CCAGTTTGGT 
TTCTTGGTGG GTCCTACCTG CTACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945666.1
CDS	47..5356
Translation	MGRGGLSAAAIRTLGHRWALLLTLLLYRGAAGEQGDTPEAGSGQMAEEVDVLESLTLHSAELVNLTLTSEDSCLALELGQYSTLSISTMTAFGDSFADELTVLMMLRSSLREDTGLLSILSLHGLILFQIRINPKAFIFISSRRGHYEFSVPTLTDGQWHRVALSISSKGVALYVDCKLVEMLSWTNYFGLGVSTDGILMLGGLIEPYEVPFKGSLQQLVFVMGEPGAAKLYCRSYNQTCLSTFSEEHFFNLSQGDLRRREARVTKISPTNTSGKVPQGRGVGTQHRGSLDSQRLINEVDLLLLEEEGFIPSFVGGEQDGAKAAKKEGQHKKLDPAEENITIEKQEKVPERLPTDKSSSDLLDLAKDPKPIPWSTPASTSAPFHSTAVPDLSLETKNKTSIDMAPTPSHATDPQKAAGNASRESNGIQTGKPKRPVARGPPGPRGSPGCPGRPGFPGPKGDKGQPGIRGRTGVTGNPGPNGPPGLPTVMIWSNSKEDWAAFYQTSFYQLLHAGWPRIRGSTGFPGMPGTPGTPGSMGPPGRPGIKGQQGSMGTLGPKGMQGMPGTPGRDGSPGLDGKPGSPGTTGAKGPQGFKGDQGPLGEKGEEGFQGSPGPSGEEGVKGAKGVKGEAGPVGPSGVQGPMGAKGSLGFPGPPGPIGDMGIPGTPGPPGPEGEQGQPGTPGTQGLNGSQGDLGPPGIIGHRGPQGPPGIEGIRGPPGPRGPQGLMGADGPQGPQGSTGDPGPMGVRGERGLEGPMGLIGWPGPDGPPGDKGRPAADAEKGDPGPKGEKGCPGSPGIKGPSGEQGHVGFVGFPGPKGQMGPIGAPGDEGQVGASGLNGSDGPKGNRGPDGPKGVTGPRGNQGRMGQRGLVGMPGASGQRGAGGPEGAEGLPGTQGPSGATGNPGSRGLTGPEGSQGDAGREGPPGQMGPPGREGIDGEPGDSGERGFTGKPGPEGPQGPVGMYGFHGPAGERGRTGYPGEKGELGPPGIQGPPGKVGLKGPPGPPGERGPPGPQGRLGEPGIQGLPGLQGPAGNPGPDGEPGKGGRPGETGPAGSIGDPGSPGYQGRDGAAGAEGPPGEKGEKGEIGPVGDQGVSGLDGEQGEPGSTGHEGSVGHKGDQGDPGPPGNPGLNGPVGDLGARGLQGLVGPPGEPGKEGDVGEIGDVGDHGPQGEKGDKGPLGSTGNPGPGGIIGERGNRGEKGDKGLSGPLGPPGLIGSTGPSGLQGEMGNVGKQGSPGLDGPQGLKGETGSTGETGQLGPPGPMGPPGKKGEKGERGQTGRPGTEGPRGINGNQGPQGFIGQEGLKGEDGSPGNTGILGVTGPRGESGPIGAPGREGKEGEKGQPGEHGVPGKPGPPGNPGQEGNVGQRGEKGDSGIDGRPGRAGNPGRKGRRGKAGPRPPRGPAGLKGLQGDKGSEGPEGPRGPYGIPGLRGTKGEPGRRGIKGELGLDGPIGLQGEEGLKGPQGPTGERGPFGIKGEQGIEGPPGSQGLPGPQGAPGPPGIEGHKGFSGSEGPRGPPGPPGLPGAPGLPGPSLNLTAQSLKRTFFHAGALSVEGPWDLLHRLEEDLKHLRSPPNGSKENPASTCKELQLVQPHLSDGHYYVDPNQGSPQDSFLVFCNFTAGGETCLLPEESQIPVRPWLQDYQASEGFQWFSTLPGGFMFRYSETGVVQLRFLRLQSSAVHQGVSYVCQGQPGVAAKDKNVKFLTDTRDQSFLVTLQGCEWEETSNRTGTLLHFAPSDLSLLPLRDLAMLHDGDIGHQFGFLVGPTCYS

Target ORF information:

RefSeq Version	XM_018090177.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-1(I) chain (LOC100495290), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090177.2

ORF Insert Sequence:

ATGGGGAGAG GGGGGCTGAG TGCTGCTGCC ATACGGACTC TCGGGCACCG CTGGGCACTG 
CTCCTCACCC TGCTGCTGTA CCGGGGGGCT GCTGGGGAGC AGGGGGACAC CCCCGAGGCT 
GGCTCTGGCC AGATGGCAGA AGAGGTGGAT GTGCTGGAAT CTCTCACTCT CCACTCAGCG 
GAGCTCGTCA ACCTCACTCT CACCTCTGAG GATTCCTGTC TCGCCCTGGA GTTGGGCCAA 
TACTCAACGC TTAGCATTTC CACCATGACA GCATTTGGGG ACAGTTTTGC AGATGAACTC 
ACCGTTCTGA TGATGCTACG ATCGTCCCTG AGAGAAGACA CCGGTCTCCT TTCCATCCTC 
AGCCTTCATG GCCTCATACT GTTCCAGATC CGAATCAACC CAAAGGCATT CATCTTCATC 
TCCAGCCGCC GAGGTCACTA TGAGTTTTCC GTGCCAACCC TCACTGACGG CCAATGGCAC 
CGGGTGGCAC TTAGTATCTC TTCTAAAGGT GTGGCGCTGT ATGTGGACTG CAAGCTGGTG 
GAGATGCTTT CTTGGACCAA CTATTTTGGA CTGGGAGTTA GCACTGATGG GATATTAATG 
CTAGGTGGGC TCATTGAACC CTACGAGGTC CCCTTCAAGG GATCGTTACA GCAACTCGTC 
TTTGTAATGG GAGAGCCGGG GGCGGCCAAA CTCTACTGCC GAAGCTACAA CCAAACTTGT 
CTGAGCACTT TCTCTGAGGA ACATTTCTTC AACTTGTCCC AGGGCGACCT CAGGAGGCGG 
GAGGCCAGAG TAACTAAGAT TTCCCCAACA AACACGAGTG GGAAGGTCCC CCAAGGACGG 
GGCGTCGGGA CACAACATAG GGGCAGTCTG GACTCTCAGC GGCTCATCAA CGAAGTGGAT 
CTGTTGCTTC TGGAAGAGGA AGGCTTCATT CCCTCCTTCG TCGGGGGCGA GCAGGACGGC 
GCCAAGGCGG CCAAGAAGGA GGGACAACAC AAAAAACTGG ACCCCGCTGA AGAGAACATC 
ACTATAGAGA AGCAAGAAAA GGTCCCCGAG CGCCTTCCGA CTGACAAATC CAGTAGTGAC 
CTCCTAGACT TGGCCAAAGA CCCCAAACCG ATTCCATGGT CTACACCTGC TTCCACCAGT 
GCCCCCTTCC ACTCCACGGC CGTTCCAGAT CTCTCTTTAG AAACAAAGAA CAAGACAAGT 
ATAGACATGG CACCTACTCC GTCCCATGCC ACCGATCCGC AGAAGGCTGC TGGGAACGCA 
AGCCGCGAGA GCAACGGCAT CCAGACAGGG AAACCCAAAA GACCCGTTGC CCGTGGGCCT 
CCGGGACCCC GTGGTAGTCC TGGCTGCCCT GGTCGTCCTG GATTTCCTGG ACCAAAAGGA 
GACAAGGGCC AACCGGGAAT AAGGGGGCGT ACAGGAGTGA CTGGCAACCC CGGTCCCAAC 
GGGCCCCCTG GACTACCCAC CGTAATGATC TGGAGCAACT CTAAAGAAGA CTGGGCAGCT 
TTCTATCAAA CGTCGTTCTA TCAGCTGCTG CACGCAGGCT GGCCTAGAAT CCGTGGGTCG 
ACCGGCTTCC CTGGGATGCC CGGAACGCCA GGAACTCCAG GTTCAATGGG ACCCCCGGGG 
CGACCTGGAA TCAAAGGACA GCAAGGTTCC ATGGGGACCC TGGGGCCCAA GGGTATGCAA 
GGGATGCCGG GGACACCAGG AAGGGACGGA TCTCCGGGAT TAGACGGGAA GCCGGGATCT 
CCGGGGACGA CAGGAGCCAA AGGCCCTCAA GGATTTAAAG GGGACCAAGG ACCTCTCGGC 
GAGAAAGGAG AAGAAGGATT TCAAGGATCT CCAGGGCCAA GTGGTGAGGA AGGAGTCAAA 
GGAGCCAAGG GGGTGAAGGG AGAGGCCGGT CCCGTGGGTC CTTCGGGGGT TCAGGGCCCA 
ATGGGTGCTA AAGGTTCTTT GGGATTTCCA GGCCCCCCAG GACCAATAGG AGATATGGGA 
ATTCCTGGAA CTCCTGGTCC ACCGGGGCCT GAGGGAGAAC AAGGACAGCC AGGAACCCCA 
GGAACCCAGG GCTTGAATGG GTCCCAGGGT GATCTGGGTC CTCCGGGAAT AATTGGTCAT 
CGGGGACCAC AGGGTCCTCC TGGAATAGAA GGTATAAGAG GACCTCCTGG GCCCAGAGGA 
CCACAGGGAT TGATGGGAGC CGATGGACCC CAAGGTCCAC AAGGAAGCAC AGGGGACCCT 
GGGCCAATGG GAGTAAGAGG AGAACGGGGA CTTGAAGGAC CCATGGGATT GATTGGATGG 
CCAGGACCAG ATGGACCTCC TGGTGATAAG GGTCGCCCTG CTGCAGATGC CGAAAAAGGA 
GATCCTGGAC CAAAGGGGGA AAAAGGTTGT CCAGGATCTC CTGGCATCAA AGGTCCTTCT 
GGAGAGCAAG GCCATGTTGG TTTTGTGGGT TTCCCAGGAC CTAAAGGTCA GATGGGCCCC 
ATCGGAGCTC CAGGGGATGA AGGCCAGGTT GGAGCTTCAG GTCTCAACGG CAGCGACGGA 
CCAAAGGGAA ACCGGGGACC TGATGGGCCA AAGGGGGTAA CAGGACCAAG AGGAAATCAG 
GGTCGTATGG GCCAGAGAGG ACTTGTAGGA ATGCCAGGAG CATCAGGTCA GCGAGGAGCC 
GGGGGCCCCG AGGGAGCAGA AGGACTTCCT GGTACACAGG GACCCAGTGG CGCAACAGGG 
AATCCTGGGA GTAGGGGGCT TACGGGTCCT GAAGGGTCTC AAGGGGACGC TGGAAGAGAA 
GGACCTCCAG GCCAAATGGG GCCTCCTGGG AGAGAAGGGA TTGATGGAGA ACCAGGAGAT 
TCTGGCGAAA GGGGATTTAC AGGGAAACCA GGCCCAGAAG GGCCACAGGG GCCAGTAGGC 
ATGTATGGGT TCCATGGCCC TGCTGGAGAG AGAGGTCGGA CGGGATACCC CGGGGAGAAG 
GGGGAATTGG GACCTCCTGG GATCCAAGGG CCACCAGGAA AAGTGGGACT AAAGGGTCCT 
CCTGGGCCAC CAGGGGAAAG AGGCCCACCA GGACCCCAGG GTCGACTAGG AGAACCAGGC 
ATCCAGGGTT TGCCCGGCCT TCAGGGCCCA GCTGGTAACC CGGGGCCAGA TGGGGAGCCT 
GGAAAGGGTG GACGTCCAGG TGAAACCGGG CCTGCTGGGA GTATAGGAGA CCCAGGATCT 
CCAGGATACC AGGGTCGAGA CGGAGCAGCA GGTGCTGAAG GACCCCCAGG AGAGAAAGGA 
GAAAAGGGAG AAATTGGGCC TGTAGGAGAC CAAGGGGTGA GCGGTTTGGA CGGGGAGCAG 
GGAGAACCTG GGTCTACTGG ACATGAAGGA TCAGTTGGCC ATAAAGGAGA TCAGGGGGAC 
CCTGGTCCTC CAGGGAATCC TGGTCTAAAC GGGCCTGTAG GAGACCTAGG GGCAAGAGGA 
CTGCAGGGCC TGGTGGGACC CCCAGGGGAA CCAGGGAAGG AGGGAGATGT GGGGGAAATT 
GGAGATGTTG GAGACCACGG TCCTCAAGGA GAAAAGGGAG ACAAAGGGCC ACTGGGTAGT 
ACCGGGAACC CAGGACCTGG CGGCATTATA GGAGAACGAG GCAATCGAGG TGAAAAGGGG 
GACAAAGGCC TGAGTGGACC TCTGGGACCC CCGGGGTTGA TTGGATCTAC AGGACCTTCT 
GGTCTACAGG GAGAAATGGG AAATGTTGGC AAACAAGGGT CCCCAGGTCT TGATGGACCT 
CAAGGTTTGA AGGGGGAAAC TGGTTCCACT GGAGAGACTG GTCAACTCGG TCCCCCTGGG 
CCAATGGGCC CACCTGGAAA GAAAGGGGAG AAGGGGGAGC GAGGACAGAC TGGGAGACCA 
GGAACAGAGG GGCCACGAGG CATTAACGGC AACCAGGGGC CACAAGGTTT TATTGGCCAA 
GAGGGACTCA AGGGGGAAGA TGGGAGCCCG GGAAATACAG GAATCCTGGG GGTTACGGGG 
CCACGTGGGG AGTCTGGACC CATAGGGGCA CCAGGAAGAG AAGGGAAGGA AGGAGAAAAG 
GGGCAACCAG GAGAACATGG AGTTCCAGGG AAACCGGGGC CCCCTGGAAA CCCAGGACAA 
GAAGGGAATG TGGGGCAGAG GGGAGAAAAG GGTGACAGTG GCATTGATGG CCGCCCTGGC 
AGAGCAGGGA ATCCTGGGAG AAAGGGCAGA AGAGGGAAAG CAGGTCCAAG GCCACCAAGA 
GGACCTGCAG GTCTCAAGGG TCTACAAGGA GATAAGGGTT CGGAAGGACC AGAAGGACCC 
AGAGGACCAT ATGGCATTCC TGGACTCAGA GGAACGAAAG GTGAACCGGG TCGAAGAGGT 
ATAAAGGGTG AGCTGGGCTT GGATGGACCA ATAGGACTGC AAGGAGAAGA AGGGCTGAAG 
GGACCACAAG GACCGACCGG GGAGCGGGGA CCTTTCGGGA TCAAAGGGGA ACAGGGAATT 
GAGGGGCCAC CTGGCTCTCA AGGATTACCT GGACCCCAAG GTGCACCAGG ACCACCAGGG 
ATAGAGGGAC ATAAAGGATT CTCTGGATCT GAAGGGCCCA GAGGGCCACC TGGACCACCG 
GGGCTTCCTG GTGCTCCTGG ACTCCCAGGA CCTTCTCTAA ACTTAACGGC CCAAAGTTTA 
AAGCGGACCT TCTTCCATGC CGGGGCCTTG AGCGTAGAGG GACCTTGGGA TCTTCTGCAT 
CGTTTGGAAG AGGACTTGAA ACACTTGCGC TCTCCGCCCA ATGGTTCCAA AGAAAACCCA 
GCCAGCACCT GTAAGGAACT TCAGCTGGTG CAACCTCATC TGTCGGATGG TCATTACTAT 
GTAGATCCCA ACCAGGGGAG TCCACAGGAT TCTTTCCTTG TGTTCTGTAA CTTCACTGCA 
GGCGGTGAGA CGTGTCTGCT CCCTGAGGAG AGCCAAATAC CTGTCAGACC TTGGCTTCAG 
GACTATCAGG CTTCTGAGGG TTTCCAGTGG TTCAGCACTC TTCCTGGGGG CTTTATGTTC 
AGGTACAGTG AAACAGGAGT CGTCCAGCTG CGGTTCCTGA GGCTACAGAG CAGCGCCGTA 
CATCAGGGGG TGAGCTACGT GTGCCAAGGT CAGCCCGGCG TCGCTGCGAA AGACAAGAAT 
GTGAAGTTTC TGACCGATAC CCGGGATCAG AGCTTTCTAG TGACTCTGCA GGGCTGTGAG 
TGGGAAGAGA CTTCAAACAG AACAGGAACT CTCCTCCACT TTGCCCCCTC AGACCTCAGC 
CTCCTTCCTC TCAGAGACTT GGCCATGTTA CACGATGGGG ACATTGGGCA CCAGTTTGGT 
TTCTTGGTGG GTCCTACCTG CTACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495290 ( XM_002943596.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002943642.2 Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X4, mRNA.

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CloneID	OXa19428
Clone ID Related Accession (Same CDS sequence)	XM_002943596.4 , XM_002943596.5
Accession Version	XM_002943596.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(I) chain-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683627.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002943596.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC CTGACATGTC CCTTAGTATC CGGAGGCTGT TGTTCCTACT CTGCTTGGCC 
CTCACCCCTA GCACTGCGGA CCCCCAGCCG ATTTCCCCAA CAAACACGAG TGGGAAGGTC 
CCCCAAGGAC GGGGCGTCGG GACACAACAT AGGGGCAGTC TGGACTCTCA GCGGCTCATC 
AACGAAGTGG ATCTGTTGCT TCTGGAAGAG GAAGGCTTCA TTCCCTCCTT CGTCGGGGGC 
GAGCAGGACG GCGCCAAGGC GGCCAAGAAG GAGGGACAAC ACAAAAAACT GGACCCCGCT 
GAAGAGAACA TCACTATAGA GAAGCAAGAA AAGGTCCCCG AGCGCCTTCC GACTGACAAA 
TCCAGTAGTG ACCTCCTAGA CTTGGCCAAA GACCCCAAAC CGATTCCATG GTCTACACCT 
GCTTCCACCA GTGCCCCCTT CCACTCCACG GCCGTTCCAG ATCTCTCTTT AGAAACAAAG 
AACAAGACAA GTATAGACAT GGCACCTACT CCGTCCCATG CCACCGATCC GCAGAAGGCT 
GCTGGGAACG CAAGCCGCGA GAGCAACGGC ATCCAGACAG GGAAACCCAA AAGACCCGTT 
GCCCGTGGGC CTCCGGGACC CCGTGGTAGT CCTGGCTGCC CTGGTCGTCC TGGATTTCCT 
GGACCAAAAG GAGACAAGGG CCAACCGGGA ATAAGGGGGC GTACAGGAGT GACTGGCAAC 
CCCGGTCCCA ACGGGCCCCC TGGACTACCC ACCGTAATGA TCTGGAGCAA CTCTAAAGAA 
GACTGGGCAG CTTTCTATCA AACGTCGTTC TATCAGCTGC TGCACGCAGG CTGGCCTAGA 
ATCCGTGGGT CGACCGGCTT CCCTGGGATG CCCGGAACGC CAGGAACTCC AGGTTCAATG 
GGACCCCCGG GGCGACCTGG AATCAAAGGA CAGCAAGGTT CCATGGGGAC CCTGGGGCCC 
AAGGGTATGC AAGGGATGCC GGGGACACCA GGAAGGGACG GATCTCCGGG ATTAGACGGG 
AAGCCGGGAT CTCCGGGGAC GACAGGAGCC AAAGGCCCTC AAGGATTTAA AGGGGACCAA 
GGACCTCTCG GCGAGAAAGG AGAAGAAGGA TTTCAAGGAT CTCCAGGGCC AAGTGGTGAG 
GAAGGAGTCA AAGGAGCCAA GGGGGTGAAG GGAGAGGCCG GTCCCGTGGG TCCTTCGGGG 
GTTCAGGGCC CAATGGGTGC TAAAGGTTCT TTGGGATTTC CAGGCCCCCC AGGACCAATA 
GGAGATATGG GAATTCCTGG AACTCCTGGT CCACCGGGGC CTGAGGGAGA ACAAGGACAG 
CCAGGAACCC CAGGAACCCA GGGCTTGAAT GGGTCCCAGG GTGATCTGGG TCCTCCGGGA 
ATAATTGGTC ATCGGGGACC ACAGGGTCCT CCTGGAATAG AAGGTATAAG AGGACCTCCT 
GGGCCCAGAG GACCACAGGG ATTGATGGGA GCCGATGGAC CCCAAGGTCC ACAAGGAAGC 
ACAGGGGACC CTGGGCCAAT GGGAGTAAGA GGAGAACGGG GACTTGAAGG ACCCATGGGA 
TTGATTGGAT GGCCAGGACC AGATGGACCT CCTGGTGATA AGGGTCGCCC TGCTGCAGAT 
GCCGAAAAAG GAGATCCTGG ACCAAAGGGG GAAAAAGGTT GTCCAGGATC TCCTGGCATC 
AAAGGTCCTT CTGGAGAGCA AGGCCATGTT GGTTTTGTGG GTTTCCCAGG ACCTAAAGGT 
CAGATGGGCC CCATCGGAGC TCCAGGGGAT GAAGGCCAGG TTGGAGCTTC AGGTCTCAAC 
GGCAGCGACG GACCAAAGGG AAACCGGGGA CCTGATGGGC CAAAGGGGGT AACAGGACCA 
AGAGGAAATC AGGGTCGTAT GGGCCAGAGA GGACTTGTAG GAATGCCAGG AGCATCAGGT 
CAGCGAGGAG CCGGGGGCCC CGAGGGAGCA GAAGGACTTC CTGGTACACA GGGACCCAGT 
GGCGCAACAG GGAATCCTGG GAGTAGGGGG CTTACGGGTC CTGAAGGGTC TCAAGGGGAC 
GCTGGAAGAG AAGGACCTCC AGGCCAAATG GGGCCTCCTG GGAGAGAAGG GATTGATGGA 
GAACCAGGAG ATTCTGGCGA AAGGGGATTT ACAGGGAAAC CAGGCCCAGA AGGGCCACAG 
GGGCCAGTAG GCATGTATGG GTTCCATGGC CCTGCTGGAG AGAGAGGTCG GACGGGATAC 
CCCGGGGAGA AGGGGGAATT GGGACCTCCT GGGATCCAAG GGCCACCAGG AAAAGTGGGA 
CTAAAGGGTC CTCCTGGGCC ACCAGGGGAA AGAGGCCCAC CAGGACCCCA GGGTCGACTA 
GGAGAACCAG GCATCCAGGG TTTGCCCGGC CTTCAGGGCC CAGCTGGTAA CCCGGGGCCA 
GATGGGGAGC CTGGAAAGGG TGGACGTCCA GGTGAAACCG GGCCTGCTGG GAGTATAGGA 
GACCCAGGAT CTCCAGGATA CCAGGGTCGA GACGGAGCAG CAGGTGCTGA AGGACCCCCA 
GGAGAGAAAG GAGAAAAGGG AGAAATTGGG CCTGTAGGAG ACCAAGGGGT GAGCGGTTTG 
GACGGGGAGC AGGGAGAACC TGGGTCTACT GGACATGAAG GATCAGTTGG CCATAAAGGA 
GATCAGGGGG ACCCTGGTCC TCCAGGGAAT CCTGGTCTAA ACGGGCCTGT AGGAGACCTA 
GGGGCAAGAG GACTGCAGGG CCTGGTGGGA CCCCCAGGGG AACCAGGGAA GGAGGGAGAT 
GTGGGGGAAA TTGGAGATGT TGGAGACCAC GGTCCTCAAG GAGAAAAGGG AGACAAAGGG 
CCACTGGGTA GTACCGGGAA CCCAGGACCT GGCGGCATTA TAGGAGAACG AGGCAATCGA 
GGTGAAAAGG GGGACAAAGG CCTGAGTGGA CCTCTGGGAC CCCCGGGGTT GATTGGATCT 
ACAGGACCTT CTGGTCTACA GGGAGAAATG GGAAATGTTG GCAAACAAGG GTCCCCAGGT 
CTTGATGGAC CTCAAGGTTT GAAGGGGGAA ACTGGTTCCA CTGGAGAGAC TGGTCAACTC 
GGTCCCCCTG GGCCAATGGG CCCACCTGGA AAGAAAGGGG AGAAGGGGGA GCGAGGACAG 
ACTGGGAGAC CAGGAACAGA GGGGCCACGA GGCATTAACG GCAACCAGGG GCCACAAGGT 
TTTATTGGCC AAGAGGGACT CAAGGGGGAA GATGGGAGCC CGGGAAATAC AGGAATCCTG 
GGGGTTACGG GGCCACGTGG GGAGTCTGGA CCCATAGGGG CACCAGGAAG AGAAGGGAAG 
GAAGGAGAAA AGGGGCAACC AGGAGAACAT GGAGTTCCAG GGAAACCGGG GCCCCCTGGA 
AACCCAGGAC AAGAAGGGAA TGTGGGGCAG AGGGGAGAAA AGGGTGACAG TGGCATTGAT 
GGCCGCCCTG GCAGAGCAGG GAATCCTGGG AGAAAGGGCA GAAGAGGGAA AGCAGGTCCA 
AGGCCACCAA GAGGACCTGC AGGTCTCAAG GGTCTACAAG GAGATAAGGG TTCGGAAGGA 
CCAGAAGGAC CCAGAGGACC ATATGGCATT CCTGGACTCA GAGGAACGAA AGGTGAACCG 
GGTCGAAGAG GTATAAAGGG TGAGCTGGGC TTGGATGGAC CAATAGGACT GCAAGGAGAA 
GAAGGGCTGA AGGGACCACA AGGACCGACC GGGGAGCGGG GACCTTTCGG GATCAAAGGG 
GAACAGGGAA TTGAGGGGCC ACCTGGCTCT CAAGGATTAC CTGGACCCCA AGGTGCACCA 
GGACCACCAG GGATAGAGGG ACATAAAGGA TTCTCTGGAT CTGAAGGGCC CAGAGGGCCA 
CCTGGACCAC CGGGGCTTCC TGGTGCTCCT GGACTCCCAG GACCTTCTCT AAACTTAACG 
GCCCAAAGTT TAAAGCGGAC CTTCTTCCAT GCCGGGGCCT TGAGCGTAGA GGGACCTTGG 
GATCTTCTGC ATCGTTTGGA AGAGGACTTG AAACACTTGC GCTCTCCGCC CAATGGTTCC 
AAAGAAAACC CAGCCAGCAC CTGTAAGGAA CTTCAGCTGG TGCAACCTCA TCTGTCGGAT 
GGTCATTACT ATGTAGATCC CAACCAGGGG AGTCCACAGG ATTCTTTCCT TGTGTTCTGT 
AACTTCACTG CAGGCGGTGA GACGTGTCTG CTCCCTGAGG AGAGCCAAAT ACCTGTCAGA 
CCTTGGCTTC AGGACTATCA GGCTTCTGAG GGTTTCCAGT GGTTCAGCAC TCTTCCTGGG 
GGCTTTATGT TCAGGTACAG TGAAACAGGA GTCGTCCAGC TGCGGTTCCT GAGGCTACAG 
AGCAGCGCCG TACATCAGGG GGTGAGCTAC GTGTGCCAAG GTCAGCCCGG CGTCGCTGCG 
AAAGACAAGA ATGTGAAGTT TCTGACCGAT ACCCGGGATC AGAGCTTTCT AGTGACTCTG 
CAGGGCTGTG AGTGGGAAGA GACTTCAAAC AGAACAGGAA CTCTCCTCCA CTTTGCCCCC 
TCAGACCTCA GCCTCCTTCC TCTCAGAGAC TTGGCCATGT TACACGATGG GGACATTGGG 
CACCAGTTTG GTTTCTTGGT GGGTCCTACC TGCTACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002943642.2
CDS	161..4762
Translation	MGSPDMSLSIRRLLFLLCLALTPSTADPQPISPTNTSGKVPQGRGVGTQHRGSLDSQRLINEVDLLLLEEEGFIPSFVGGEQDGAKAAKKEGQHKKLDPAEENITIEKQEKVPERLPTDKSSSDLLDLAKDPKPIPWSTPASTSAPFHSTAVPDLSLETKNKTSIDMAPTPSHATDPQKAAGNASRESNGIQTGKPKRPVARGPPGPRGSPGCPGRPGFPGPKGDKGQPGIRGRTGVTGNPGPNGPPGLPTVMIWSNSKEDWAAFYQTSFYQLLHAGWPRIRGSTGFPGMPGTPGTPGSMGPPGRPGIKGQQGSMGTLGPKGMQGMPGTPGRDGSPGLDGKPGSPGTTGAKGPQGFKGDQGPLGEKGEEGFQGSPGPSGEEGVKGAKGVKGEAGPVGPSGVQGPMGAKGSLGFPGPPGPIGDMGIPGTPGPPGPEGEQGQPGTPGTQGLNGSQGDLGPPGIIGHRGPQGPPGIEGIRGPPGPRGPQGLMGADGPQGPQGSTGDPGPMGVRGERGLEGPMGLIGWPGPDGPPGDKGRPAADAEKGDPGPKGEKGCPGSPGIKGPSGEQGHVGFVGFPGPKGQMGPIGAPGDEGQVGASGLNGSDGPKGNRGPDGPKGVTGPRGNQGRMGQRGLVGMPGASGQRGAGGPEGAEGLPGTQGPSGATGNPGSRGLTGPEGSQGDAGREGPPGQMGPPGREGIDGEPGDSGERGFTGKPGPEGPQGPVGMYGFHGPAGERGRTGYPGEKGELGPPGIQGPPGKVGLKGPPGPPGERGPPGPQGRLGEPGIQGLPGLQGPAGNPGPDGEPGKGGRPGETGPAGSIGDPGSPGYQGRDGAAGAEGPPGEKGEKGEIGPVGDQGVSGLDGEQGEPGSTGHEGSVGHKGDQGDPGPPGNPGLNGPVGDLGARGLQGLVGPPGEPGKEGDVGEIGDVGDHGPQGEKGDKGPLGSTGNPGPGGIIGERGNRGEKGDKGLSGPLGPPGLIGSTGPSGLQGEMGNVGKQGSPGLDGPQGLKGETGSTGETGQLGPPGPMGPPGKKGEKGERGQTGRPGTEGPRGINGNQGPQGFIGQEGLKGEDGSPGNTGILGVTGPRGESGPIGAPGREGKEGEKGQPGEHGVPGKPGPPGNPGQEGNVGQRGEKGDSGIDGRPGRAGNPGRKGRRGKAGPRPPRGPAGLKGLQGDKGSEGPEGPRGPYGIPGLRGTKGEPGRRGIKGELGLDGPIGLQGEEGLKGPQGPTGERGPFGIKGEQGIEGPPGSQGLPGPQGAPGPPGIEGHKGFSGSEGPRGPPGPPGLPGAPGLPGPSLNLTAQSLKRTFFHAGALSVEGPWDLLHRLEEDLKHLRSPPNGSKENPASTCKELQLVQPHLSDGHYYVDPNQGSPQDSFLVFCNFTAGGETCLLPEESQIPVRPWLQDYQASEGFQWFSTLPGGFMFRYSETGVVQLRFLRLQSSAVHQGVSYVCQGQPGVAAKDKNVKFLTDTRDQSFLVTLQGCEWEETSNRTGTLLHFAPSDLSLLPLRDLAMLHDGDIGHQFGFLVGPTCYS

Target ORF information:

RefSeq Version	XM_002943596.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen alpha-1(I) chain-like (LOC100495290), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002943596.4

ORF Insert Sequence:

ATGGGGAGCC CTGACATGTC CCTTAGTATC CGGAGGCTGT TGTTCCTACT CTGCTTGGCC 
CTCACCCCTA GCACTGCGGA CCCCCAGCCG ATTTCCCCAA CAAACACGAG TGGGAAGGTC 
CCCCAAGGAC GGGGCGTCGG GACACAACAT AGGGGCAGTC TGGACTCTCA GCGGCTCATC 
AACGAAGTGG ATCTGTTGCT TCTGGAAGAG GAAGGCTTCA TTCCCTCCTT CGTCGGGGGC 
GAGCAGGACG GCGCCAAGGC GGCCAAGAAG GAGGGACAAC ACAAAAAACT GGACCCCGCT 
GAAGAGAACA TCACTATAGA GAAGCAAGAA AAGGTCCCCG AGCGCCTTCC GACTGACAAA 
TCCAGTAGTG ACCTCCTAGA CTTGGCCAAA GACCCCAAAC CGATTCCATG GTCTACACCT 
GCTTCCACCA GTGCCCCCTT CCACTCCACG GCCGTTCCAG ATCTCTCTTT AGAAACAAAG 
AACAAGACAA GTATAGACAT GGCACCTACT CCGTCCCATG CCACCGATCC GCAGAAGGCT 
GCTGGGAACG CAAGCCGCGA GAGCAACGGC ATCCAGACAG GGAAACCCAA AAGACCCGTT 
GCCCGTGGGC CTCCGGGACC CCGTGGTAGT CCTGGCTGCC CTGGTCGTCC TGGATTTCCT 
GGACCAAAAG GAGACAAGGG CCAACCGGGA ATAAGGGGGC GTACAGGAGT GACTGGCAAC 
CCCGGTCCCA ACGGGCCCCC TGGACTACCC ACCGTAATGA TCTGGAGCAA CTCTAAAGAA 
GACTGGGCAG CTTTCTATCA AACGTCGTTC TATCAGCTGC TGCACGCAGG CTGGCCTAGA 
ATCCGTGGGT CGACCGGCTT CCCTGGGATG CCCGGAACGC CAGGAACTCC AGGTTCAATG 
GGACCCCCGG GGCGACCTGG AATCAAAGGA CAGCAAGGTT CCATGGGGAC CCTGGGGCCC 
AAGGGTATGC AAGGGATGCC GGGGACACCA GGAAGGGACG GATCTCCGGG ATTAGACGGG 
AAGCCGGGAT CTCCGGGGAC GACAGGAGCC AAAGGCCCTC AAGGATTTAA AGGGGACCAA 
GGACCTCTCG GCGAGAAAGG AGAAGAAGGA TTTCAAGGAT CTCCAGGGCC AAGTGGTGAG 
GAAGGAGTCA AAGGAGCCAA GGGGGTGAAG GGAGAGGCCG GTCCCGTGGG TCCTTCGGGG 
GTTCAGGGCC CAATGGGTGC TAAAGGTTCT TTGGGATTTC CAGGCCCCCC AGGACCAATA 
GGAGATATGG GAATTCCTGG AACTCCTGGT CCACCGGGGC CTGAGGGAGA ACAAGGACAG 
CCAGGAACCC CAGGAACCCA GGGCTTGAAT GGGTCCCAGG GTGATCTGGG TCCTCCGGGA 
ATAATTGGTC ATCGGGGACC ACAGGGTCCT CCTGGAATAG AAGGTATAAG AGGACCTCCT 
GGGCCCAGAG GACCACAGGG ATTGATGGGA GCCGATGGAC CCCAAGGTCC ACAAGGAAGC 
ACAGGGGACC CTGGGCCAAT GGGAGTAAGA GGAGAACGGG GACTTGAAGG ACCCATGGGA 
TTGATTGGAT GGCCAGGACC AGATGGACCT CCTGGTGATA AGGGTCGCCC TGCTGCAGAT 
GCCGAAAAAG GAGATCCTGG ACCAAAGGGG GAAAAAGGTT GTCCAGGATC TCCTGGCATC 
AAAGGTCCTT CTGGAGAGCA AGGCCATGTT GGTTTTGTGG GTTTCCCAGG ACCTAAAGGT 
CAGATGGGCC CCATCGGAGC TCCAGGGGAT GAAGGCCAGG TTGGAGCTTC AGGTCTCAAC 
GGCAGCGACG GACCAAAGGG AAACCGGGGA CCTGATGGGC CAAAGGGGGT AACAGGACCA 
AGAGGAAATC AGGGTCGTAT GGGCCAGAGA GGACTTGTAG GAATGCCAGG AGCATCAGGT 
CAGCGAGGAG CCGGGGGCCC CGAGGGAGCA GAAGGACTTC CTGGTACACA GGGACCCAGT 
GGCGCAACAG GGAATCCTGG GAGTAGGGGG CTTACGGGTC CTGAAGGGTC TCAAGGGGAC 
GCTGGAAGAG AAGGACCTCC AGGCCAAATG GGGCCTCCTG GGAGAGAAGG GATTGATGGA 
GAACCAGGAG ATTCTGGCGA AAGGGGATTT ACAGGGAAAC CAGGCCCAGA AGGGCCACAG 
GGGCCAGTAG GCATGTATGG GTTCCATGGC CCTGCTGGAG AGAGAGGTCG GACGGGATAC 
CCCGGGGAGA AGGGGGAATT GGGACCTCCT GGGATCCAAG GGCCACCAGG AAAAGTGGGA 
CTAAAGGGTC CTCCTGGGCC ACCAGGGGAA AGAGGCCCAC CAGGACCCCA GGGTCGACTA 
GGAGAACCAG GCATCCAGGG TTTGCCCGGC CTTCAGGGCC CAGCTGGTAA CCCGGGGCCA 
GATGGGGAGC CTGGAAAGGG TGGACGTCCA GGTGAAACCG GGCCTGCTGG GAGTATAGGA 
GACCCAGGAT CTCCAGGATA CCAGGGTCGA GACGGAGCAG CAGGTGCTGA AGGACCCCCA 
GGAGAGAAAG GAGAAAAGGG AGAAATTGGG CCTGTAGGAG ACCAAGGGGT GAGCGGTTTG 
GACGGGGAGC AGGGAGAACC TGGGTCTACT GGACATGAAG GATCAGTTGG CCATAAAGGA 
GATCAGGGGG ACCCTGGTCC TCCAGGGAAT CCTGGTCTAA ACGGGCCTGT AGGAGACCTA 
GGGGCAAGAG GACTGCAGGG CCTGGTGGGA CCCCCAGGGG AACCAGGGAA GGAGGGAGAT 
GTGGGGGAAA TTGGAGATGT TGGAGACCAC GGTCCTCAAG GAGAAAAGGG AGACAAAGGG 
CCACTGGGTA GTACCGGGAA CCCAGGACCT GGCGGCATTA TAGGAGAACG AGGCAATCGA 
GGTGAAAAGG GGGACAAAGG CCTGAGTGGA CCTCTGGGAC CCCCGGGGTT GATTGGATCT 
ACAGGACCTT CTGGTCTACA GGGAGAAATG GGAAATGTTG GCAAACAAGG GTCCCCAGGT 
CTTGATGGAC CTCAAGGTTT GAAGGGGGAA ACTGGTTCCA CTGGAGAGAC TGGTCAACTC 
GGTCCCCCTG GGCCAATGGG CCCACCTGGA AAGAAAGGGG AGAAGGGGGA GCGAGGACAG 
ACTGGGAGAC CAGGAACAGA GGGGCCACGA GGCATTAACG GCAACCAGGG GCCACAAGGT 
TTTATTGGCC AAGAGGGACT CAAGGGGGAA GATGGGAGCC CGGGAAATAC AGGAATCCTG 
GGGGTTACGG GGCCACGTGG GGAGTCTGGA CCCATAGGGG CACCAGGAAG AGAAGGGAAG 
GAAGGAGAAA AGGGGCAACC AGGAGAACAT GGAGTTCCAG GGAAACCGGG GCCCCCTGGA 
AACCCAGGAC AAGAAGGGAA TGTGGGGCAG AGGGGAGAAA AGGGTGACAG TGGCATTGAT 
GGCCGCCCTG GCAGAGCAGG GAATCCTGGG AGAAAGGGCA GAAGAGGGAA AGCAGGTCCA 
AGGCCACCAA GAGGACCTGC AGGTCTCAAG GGTCTACAAG GAGATAAGGG TTCGGAAGGA 
CCAGAAGGAC CCAGAGGACC ATATGGCATT CCTGGACTCA GAGGAACGAA AGGTGAACCG 
GGTCGAAGAG GTATAAAGGG TGAGCTGGGC TTGGATGGAC CAATAGGACT GCAAGGAGAA 
GAAGGGCTGA AGGGACCACA AGGACCGACC GGGGAGCGGG GACCTTTCGG GATCAAAGGG 
GAACAGGGAA TTGAGGGGCC ACCTGGCTCT CAAGGATTAC CTGGACCCCA AGGTGCACCA 
GGACCACCAG GGATAGAGGG ACATAAAGGA TTCTCTGGAT CTGAAGGGCC CAGAGGGCCA 
CCTGGACCAC CGGGGCTTCC TGGTGCTCCT GGACTCCCAG GACCTTCTCT AAACTTAACG 
GCCCAAAGTT TAAAGCGGAC CTTCTTCCAT GCCGGGGCCT TGAGCGTAGA GGGACCTTGG 
GATCTTCTGC ATCGTTTGGA AGAGGACTTG AAACACTTGC GCTCTCCGCC CAATGGTTCC 
AAAGAAAACC CAGCCAGCAC CTGTAAGGAA CTTCAGCTGG TGCAACCTCA TCTGTCGGAT 
GGTCATTACT ATGTAGATCC CAACCAGGGG AGTCCACAGG ATTCTTTCCT TGTGTTCTGT 
AACTTCACTG CAGGCGGTGA GACGTGTCTG CTCCCTGAGG AGAGCCAAAT ACCTGTCAGA 
CCTTGGCTTC AGGACTATCA GGCTTCTGAG GGTTTCCAGT GGTTCAGCAC TCTTCCTGGG 
GGCTTTATGT TCAGGTACAG TGAAACAGGA GTCGTCCAGC TGCGGTTCCT GAGGCTACAG 
AGCAGCGCCG TACATCAGGG GGTGAGCTAC GTGTGCCAAG GTCAGCCCGG CGTCGCTGCG 
AAAGACAAGA ATGTGAAGTT TCTGACCGAT ACCCGGGATC AGAGCTTTCT AGTGACTCTG 
CAGGGCTGTG AGTGGGAAGA GACTTCAAAC AGAACAGGAA CTCTCCTCCA CTTTGCCCCC 
TCAGACCTCA GCCTCCTTCC TCTCAGAGAC TTGGCCATGT TACACGATGG GGACATTGGG 
CACCAGTTTG GTTTCTTGGT GGGTCCTACC TGCTACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC100495290 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報