si:ch211-196i2.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	si:ch211-196i2.1
Entrez Gene ID	564099
Full Name	si:ch211-196i2.1
General protein information	Preferred Names si:ch211-196i2.1 Names collagen alpha-1(I) chain-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009295266.2	XP_009293541.1	collagen alpha-1(V) chain-like isoform X4
XM_009295262.2	XP_009293537.1	collagen alpha-1(I) chain-like isoform X2
XM_009295262.3	XP_009293537.1	collagen alpha-1(I) chain-like isoform X2
XM_021469035.1	XP_021324710.1	collagen alpha-1(I) chain-like isoform X3
XM_009295261.3	XP_009293536.1	collagen alpha-1(I) chain-like isoform X1
XM_009295261.2	XP_009293536.1	collagen alpha-1(I) chain-like isoform X1
XM_009295265.1	XP_009293540.1	collagen alpha-1(V) chain-like isoform X3
XM_021473390.1	XP_021329065.1	collagen alpha-1(I) chain-like isoform X4

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100495290	XP_002943642.2
Danio rerio (zebrafish)	LOC564099	XP_692548.3
Gallus gallus (chicken)	LOC101747382	XP_004945850.1

Gene Ontology

The following si:ch211-196i2.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:ch211-196i2.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa44246	XM_009295266.2 Latest version!	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa44244	XM_021469035.1 Latest version!	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa44245	XM_009295265.1 Latest version!	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa146092	XM_021473390.1 Latest version!	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa44242	XM_009295261.3 Latest version!	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44243	XM_009295262.3 Latest version!	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa44243	XM_009295262.2	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44242	XM_009295261.2	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

si:ch211-196i2.1 ( XM_009295266.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293541.1 Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa44246
Clone ID Related Accession (Same CDS sequence)	XM_009295266.2
Accession Version	XM_009295266.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(V) chain-like isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGTGT CAGTAGTGTG GACAGCAGTT TTATTTACTC TGGTAACAGC CTCCATCACG 
GACACCAGTC CTACTGACGT ACCAGACACG GAGAGCGGTT CGGGGATGTT TCTACCAGAT 
GCAATCGACC TTCTTGATCA TCTATGGCAG GTTGCTAATC ACAGTAATGC ATCGGTAACG 
CATGAAAGCA AAAGGTGTCC AGTTCTGCAT GTAGGCCAGT ACTCAACACT TTCTGTCCCA 
CTACGGCAGG TGTTCGGATA CAGTTTTGGT GATGAGTTCA GTTTGCTGTT GCATCTGCGC 
AGCTCTCAGT CTGAAGACCG CAGTGTGTTG ACCTTCCTCT GTCCAGATGG ACACATTCTT 
CTGCAGATCC GCATCAGTGC CTACACTTTT AGCTTCATCA GCACTCAACA GCGACACTAC 
GAATTCCCCG TGGGAGTACT GTCTGATGGA GGTTGGCACC GTGTGGCAGT CAGCGTTTCC 
TCGAGTCGGT TGGCATTGTA TGTGGATTGC TCCATGGTGG AGAGTGTGGA ATGGGCCTAT 
AAGGGCGGTC TGGAAATCTC CACCGATGGC CTGGTTATGT TGGGAGGAAT CATTGAGGGG 
TTTGAGACCC CTTTTGAGGG CGATTTGATG CAAGTGGCAT TTCTGATTGG AGAACCAGAT 
GCAGCTCAAG ATCACTGTGC CTTTTTCCAG CCCCAGTGTG GTGGAACTGG ACACAAACCA 
CCTCGCTCTC CTCGCATACA CACTGAAGAG GAACTTTTGC TATCCTCTAA TGACCTGGAG 
GACTTGTTAG AGTCAGCAAA GAACAGTGAC TCTATTCGGG CAAAACTGAA TGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGGAC TGCAAGGCCT 
TGTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293541.1
CDS	167..2995
Translation	MLVSVVWTAVLFTLVTASITDTSPTDVPDTESGSGMFLPDAIDLLDHLWQVANHSNASVTHESKRCPVLHVGQYSTLSVPLRQVFGYSFGDEFSLLLHLRSSQSEDRSVLTFLCPDGHILLQIRISAYTFSFISTQQRHYEFPVGVLSDGGWHRVAVSVSSSRLALYVDCSMVESVEWAYKGGLEISTDGLVMLGGIIEGFETPFEGDLMQVAFLIGEPDAAQDHCAFFQPQCGGTGHKPPRSPRIHTEEELLLSSNDLEDLLESAKNSDSIRAKLNGLRRRGIIRGDGTLPTGSKRPAIVGRGDVFEVDEDTDLLDQNILSKNGDKPSKTFPTDTTSSRDGKLESTSKHLDENITIDKHKPGKPLPKKPVDTTIINLDVSEKPTGSTVPSREIHLMTTSSPEDGESNLLKEDFEVTTQAPGRTSWDNVSKGSGRTPSKSPDIHAGSGGGSEEHPDTIVLTGRQGDIVRGSDGKMYRIQRGPMGPMGPPGEPGCPGNAGYPGPKGDKGAFGVRGRPGRPGFLGSPGPPGLPSFYLWRNTPEDWAAFQQTSFFQLLLAGWPRAQGLPGPEGEMGKPGAQGPPGEPGERGPPGRMGDMGDRGPKGVVGRAGVYGRDGENGLDGPQGPSGIPGPKGPLGYKGESGSHGEKGEEGITGSEGPCGDKGKPGEKGSKGAPGAPGAVGPPGPQGIRGMEGLEGHPGPDGEDGMNGSPGLPGAPGAPGWTGLIGAQGANGSRGEPGPSGAVGLPGPQGPQGLEGQIGPPGQRGPPGLSGREGLHGPKGEPGPVGPVGIRGDPGFEGLKGVLGTPGPKGFTGIKGNRGPDGDQGDIGPKGNKGARGAAGVQGIQGEQGPIGFPGFPGIRGPPGPQGNQGEDGEAGPHGKVGKEGSTGSRGPEGLSGKPGPRGLKGRTGQRGHTGMPGPPGLPGPPGPFGAEGKPGTARPCG

Target ORF information:

RefSeq Version	XM_009295266.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295266.2

ORF Insert Sequence:

ATGCTCGTGT CAGTAGTGTG GACAGCAGTT TTATTTACTC TGGTAACAGC CTCCATCACG 
GACACCAGTC CTACTGACGT ACCAGACACG GAGAGCGGTT CGGGGATGTT TCTACCAGAT 
GCAATCGACC TTCTTGATCA TCTATGGCAG GTTGCTAATC ACAGTAATGC ATCGGTAACG 
CATGAAAGCA AAAGGTGTCC AGTTCTGCAT GTAGGCCAGT ACTCAACACT TTCTGTCCCA 
CTACGGCAGG TGTTCGGATA CAGTTTTGGT GATGAGTTCA GTTTGCTGTT GCATCTGCGC 
AGCTCTCAGT CTGAAGACCG CAGTGTGTTG ACCTTCCTCT GTCCAGATGG ACACATTCTT 
CTGCAGATCC GCATCAGTGC CTACACTTTT AGCTTCATCA GCACTCAACA GCGACACTAC 
GAATTCCCCG TGGGAGTACT GTCTGATGGA GGTTGGCACC GTGTGGCAGT CAGCGTTTCC 
TCGAGTCGGT TGGCATTGTA TGTGGATTGC TCCATGGTGG AGAGTGTGGA ATGGGCCTAT 
AAGGGCGGTC TGGAAATCTC CACCGATGGC CTGGTTATGT TGGGAGGAAT CATTGAGGGG 
TTTGAGACCC CTTTTGAGGG CGATTTGATG CAAGTGGCAT TTCTGATTGG AGAACCAGAT 
GCAGCTCAAG ATCACTGTGC CTTTTTCCAG CCCCAGTGTG GTGGAACTGG ACACAAACCA 
CCTCGCTCTC CTCGCATACA CACTGAAGAG GAACTTTTGC TATCCTCTAA TGACCTGGAG 
GACTTGTTAG AGTCAGCAAA GAACAGTGAC TCTATTCGGG CAAAACTGAA TGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGGAC TGCAAGGCCT 
TGTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-196i2.1 ( XM_021469035.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324710.1 Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa44244
Clone ID Related Accession (Same CDS sequence)	XM_021469035.1
Accession Version	XM_021469035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(I) chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCAAA AGGCAGTGGA AGGACACCAT CGAAATCTCC AGATATTCAT GCAGGATCTG 
GAGGGGGATC TGAGGAGCAT CCGGATACCA TTGTGCTTAC AGGCAGACAA GGAGACATTG 
TGCGTGGGTC TGATGGGAAA ATGTATCGCA TCCAAAGAGG GCCTATGGGG CCAATGGGGC 
CCCCAGGTGA GCCTGGGTGT CCTGGGAATG CTGGCTATCC TGGACCCAAA GGAGATAAGG 
GTGCCTTTGG TGTTAGGGGT CGTCCAGGTC GCCCTGGATT CCTGGGATCC CCAGGACCAC 
CCGGCCTGCC CTCATTCTAC CTATGGAGAA ACACACCTGA AGACTGGGCT GCATTTCAGC 
AAACATCATT CTTCCAGCTT CTTCTTGCAG GCTGGCCTAG AGCACAAGGT CTGCCTGGTC 
CGGAAGGAGA GATGGGAAAA CCTGGGGCAC AGGGGCCACC TGGGGAACCA GGTGAACGAG 
GACCCCCAGG ACGAATGGGA GACATGGGTG ACCGTGGTCC TAAAGGTGTG GTTGGAAGAG 
CGGGAGTTTA TGGAAGAGAT GGAGAGAACG GCCTGGACGG CCCTCAGGGG CCTTCAGGGA 
TTCCAGGCCC AAAGGGTCCT CTGGGTTATA AAGGTGAATC AGGCTCACAT GGAGAGAAAG 
GAGAGGAGGG CATTACTGGA TCAGAGGGTC CATGTGGAGA TAAGGGGAAA CCCGGGTTCA 
AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC GGCCCTCCTG GTCCTCAAGG CATAAGAGGG 
ATGGAGGGCC TTGAAGGGCA TCCAGGACCA GATGGGGAGG ATGGGATGAA TGGATCACCA 
GGTCTTCCAG GTGCACCAGG AGCTCCTGGT TGGACAGGTT TGATTGGTGC CCAGGGGGCT 
AATGGGTCTC GTGGGGAACC TGGGCCTTCT GGAGCTGTTG GACTTCCAGG GCCACAGGGG 
CCCCAGGGAT TAGAAGGACA GATCGGACCT CCAGGACAAA GAGGTCCACC GGGGTTGTCA 
GGCCGAGAGG GTTTGCATGG TCCCAAAGGA GAGCCTGGTC CTGTAGGACC AGTTGGTATC 
AGAGGGGATC CAGGATTTGA AGGATTAAAG GGTGTTTTAG GAACGCCCGG TCCAAAAGGT 
TTCACTGGTA TCAAAGGTAA CAGAGGACCT GATGGAGACC AAGGGGACAT TGGACCTAAA 
GGAAACAAAG GTGCTAGAGG AGCAGCAGGT GTACAGGGCA TCCAAGGTGA ACAGGGACCA 
ATAGGCTTTC CTGGCTTTCC AGGCATCAGA GGCCCACCAG GACCACAGGG AAACCAGGGA 
GAGGATGGAG AGGCTGGTCC ACATGGTAAA GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA 
GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT CCTAGAGGCC TAAAGGGGCG AACAGGGCAG 
AGGGGTCACA CTGGCATGCC TGGACCTCCT GGTTTACCTG GCCCACCTGG ACCATTCGGA 
GCTGAAGGAA AGCCTGGTAC ACAGGGACTG CAAGGCCTTG TGGGTAATGC CGGAAATAAA 
GGATCAACGG GATTGCAAGG TATTCCCGGA GAACGTGGTC AAGACGGACC CCCAGGAACT 
ATGGGCCCAC CTGGACGAGA GGGGCCTATG GGCAGCCCAG GACCACCAGG AGAAAGGGGT 
TTTGGTGGAA AGACAGGAAT GAAGGGACCA CAGGGCCCTG TTGGCATGTA TGGTTTTCCA 
GGAACCGTGG GTATGAGAGG AAGACCAGGC CACACTGGAG ACAGAGGAGA GCCAGGCCCA 
AGAGGCCCTA CTGGACCAAT TGGAAAGACT GGCGTACCAG GTCCCCCTGG TCCAGTAGGA 
GAAAAAGGAC TGCCAGGACC CCAGGGCCGC CAAGGAGATC CTGGGCATCA GGGAACAGAT 
GGCCCATGTG GTCCTCCAGG ACGTCACGGA GTCAGTGGGG TCATTGGAAA ACCAGGACCT 
CGTGGAAGTA TAGGTCCCCC AGGGGAACCT GGGCCAAGTG GGCCCCCAGG AATACCAGGT 
CCACAGGGGG AAAACGGAGT GCCAGGTATA GATGGAGAGA AAGGAGAAAA GGGAATGATG 
GGTTCTCCAG GTGATGATGG ACCTCTTGGA CTGGAAGGAC AACAGGGTCT AACTGGTCCT 
GCAGGTTTGG ATGGGGCTCC TGGAAGGAAG GGTGATAAGG GGGATAGAGG ACCTATCGGA 
ACGTCTGGTC TTCAAGGACC AAAAGGCGAT ATTGGCCCTG TAGGTGATAA AGGTCCACCG 
GGTCCACCAG GCCTAGTAGG CAATGAGGGT GATTCTGGTC CTGCTGGTGT TGCTGGTGAC 
CCCGGGTCAA AAGGAGAAAA GGGTGATATT GGGCCACCAG GATTTACTGG TTTAGAGGGT 
CCATGGGGAG ACACAGGGGA GCAGGGAGAG AAGGGGATCA AGGGAGCCAA GGGACAAATC 
GGAATGCAGG GTGAGCCAGG CCTAGTTGGT GGGGTTGGAC ATCCTGGCCT TAAAGGTGCA 
GAAGGTGTAC CTGGCCATCC AGGACTGGTT GGGCCAGATG GACCTGCAGG AAGCAAGGGA 
AAGACCGGGC CAAGTGGTTC ACCTGGTGAG CCAGGAAATC CTGGACCTGA GGGAATTGCT 
GGTCCCAAAG GTGTGCGAGG TGACACAGGA AAAGGCGGGA CACAGGGTGG TGTGGGTCTA 
GTTGGTCCCA TAGGTCCTTT GGGCCCCAAA GGTCTTTCAG GGCCTGAAGG CGAGAAAGGA 
GAGCCAGGAG ATAAAGGAGA ACCAGGGTTA GAGGGACCAG ATGGCCTTCA GGGTGTACAA 
GGACCACCAG GACTGCCTGG TTTGGAGGGT TCGCAAGGTG AGGCAGGGAT GAGGGGCCCA 
CCAGGTCTGC CAGGAGCTCA GGGCGGCGTG GGTAAGCCAG GACCTGACGG AAGAGAAGGC 
ATGAAAGGAG AGAAGGGGGA TCAAGGTAAA AATGGAGCAC CTGGAAAATT GGGTCACATT 
GGACGAAGGG GTAAAAGAGG CAAGGCAGGT TTGAGGGCGA CCAGAGGACC AAGGGGAGAA 
AAGGGAGAGA AAGGAGATGT TGGTGAGAAA GGACTTCCTG GATGGGGAGG GAGTATGGGA 
ATCCAAGGGC CAAGAGGAGA GCCTGGAGAT GAAGGTGTAA AAGGAGCAGA GGGTGAGAAA 
GGGGATCAGG GACCATTTGG TTCACCTGGG AGAGATGGCA TAAAGGGAAG TCTTGGACCT 
CTTGGCCCTC CTGGACCCCC AGGACCAAAG GGTGATCAGG GAAATATTGG TCCTTCTGGT 
CCTAGAGGGA CACCTGGAAT ACCTGGACTG CCGGGCTTGT TTGGAGACAA GGGACTGAAA 
GGATTTCAAG GGCCTCCTGG ACCCCCTGGA ACAAGGGGTC TGCCAGGTCC ACCAGGGCCA 
CCAGGACCTC CTGGTATCTC TGTGAACCTG ACTCTGATCC AGATCAAGGA CCTGATGTAC 
AGTTCAGACA AGCCCAATTT CTCCCTGATA CAGACACTGC TGGACTCACT GTACCATGAC 
CTCCAGCTGC TGGTGGACCC TCCGGACGGC ACTAAGCAGC ACCCAGCATC AACCTGTCTG 
GAGCTGTGGC TGTGCCATCC CAACTACACG AGTGGTTTCT ACTACATTGA TCCAAACCAA 
GGCAGTCCTT TAGATGCACT TTTAGCCTAC TGTAATTTCT CTGAGTCCGG AGCAGAGACC 
TGTCTTCACC CGAGAGATGC ACATCTGCCC ACAAGGACTT GGCTAAATGA CTCAGGAAAT 
AACAGCAAGT TCCACTGGTT AAGCTCACTA GAGGGGGGTT TTAAATTTGA GTATCCAGCC 
CTAAGTGTGG TTCAGCTGCG CTTTCTGAGA CTCCAGAGCA ACAGAGCGGA GCAGAGAGTG 
ACGTACTCCT GTTACCCTGG ACACAGACTG GGAGAAACAC AGCGACAGGT CCAGTTCCTC 
ACAGACGCCG CCAAGCAGAG CTATTTAGGA GCACTGCAGG ACTGTGTGTC TGCAAAGGAA 
GTGGATCCTG GGCCGCGTGA GTCTGTGTTT CAGTTTGAGG ACATGGACCT GCTTCCTCTA 
CGTGATATAG GGATCTTTGG AAACAGCAGT CAAGAGTTTG GCTTCACCAT TGGCCCTGTA 
TGCTTCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324710.1
CDS	133..4284
Translation	MCQKAVEGHHRNLQIFMQDLEGDLRSIRIPLCLQADKETLCVGLMGKCIASKEGLWGQWGPQVSLGVLGMLAILDPKEIRVPLVLGVVQVALDSWDPQDHPACPHSTYGETHLKTGLHFSKHHSSSFFLQAGLEHKVCLVRKERWENLGHRGHLGNQVNEDPQDEWETWVTVVLKVWLEEREFMEEMERTAWTALRGLQGFQAQRVLWVIKVNQAHMERKERRALLDQRVHVEIRGNPGSKGAPGAPGAVGPPGPQGIRGMEGLEGHPGPDGEDGMNGSPGLPGAPGAPGWTGLIGAQGANGSRGEPGPSGAVGLPGPQGPQGLEGQIGPPGQRGPPGLSGREGLHGPKGEPGPVGPVGIRGDPGFEGLKGVLGTPGPKGFTGIKGNRGPDGDQGDIGPKGNKGARGAAGVQGIQGEQGPIGFPGFPGIRGPPGPQGNQGEDGEAGPHGKVGKEGSTGSRGPEGLSGKPGPRGLKGRTGQRGHTGMPGPPGLPGPPGPFGAEGKPGTQGLQGLVGNAGNKGSTGLQGIPGERGQDGPPGTMGPPGREGPMGSPGPPGERGFGGKTGMKGPQGPVGMYGFPGTVGMRGRPGHTGDRGEPGPRGPTGPIGKTGVPGPPGPVGEKGLPGPQGRQGDPGHQGTDGPCGPPGRHGVSGVIGKPGPRGSIGPPGEPGPSGPPGIPGPQGENGVPGIDGEKGEKGMMGSPGDDGPLGLEGQQGLTGPAGLDGAPGRKGDKGDRGPIGTSGLQGPKGDIGPVGDKGPPGPPGLVGNEGDSGPAGVAGDPGSKGEKGDIGPPGFTGLEGPWGDTGEQGEKGIKGAKGQIGMQGEPGLVGGVGHPGLKGAEGVPGHPGLVGPDGPAGSKGKTGPSGSPGEPGNPGPEGIAGPKGVRGDTGKGGTQGGVGLVGPIGPLGPKGLSGPEGEKGEPGDKGEPGLEGPDGLQGVQGPPGLPGLEGSQGEAGMRGPPGLPGAQGGVGKPGPDGREGMKGEKGDQGKNGAPGKLGHIGRRGKRGKAGLRATRGPRGEKGEKGDVGEKGLPGWGGSMGIQGPRGEPGDEGVKGAEGEKGDQGPFGSPGRDGIKGSLGPLGPPGPPGPKGDQGNIGPSGPRGTPGIPGLPGLFGDKGLKGFQGPPGPPGTRGLPGPPGPPGPPGISVNLTLIQIKDLMYSSDKPNFSLIQTLLDSLYHDLQLLVDPPDGTKQHPASTCLELWLCHPNYTSGFYYIDPNQGSPLDALLAYCNFSESGAETCLHPRDAHLPTRTWLNDSGNNSKFHWLSSLEGGFKFEYPALSVVQLRFLRLQSNRAEQRVTYSCYPGHRLGETQRQVQFLTDAAKQSYLGALQDCVSAKEVDPGPRESVFQFEDMDLLPLRDIGIFGNSSQEFGFTIGPVCFS

Target ORF information:

RefSeq Version	XM_021469035.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469035.1

ORF Insert Sequence:

ATGTGTCAAA AGGCAGTGGA AGGACACCAT CGAAATCTCC AGATATTCAT GCAGGATCTG 
GAGGGGGATC TGAGGAGCAT CCGGATACCA TTGTGCTTAC AGGCAGACAA GGAGACATTG 
TGCGTGGGTC TGATGGGAAA ATGTATCGCA TCCAAAGAGG GCCTATGGGG CCAATGGGGC 
CCCCAGGTGA GCCTGGGTGT CCTGGGAATG CTGGCTATCC TGGACCCAAA GGAGATAAGG 
GTGCCTTTGG TGTTAGGGGT CGTCCAGGTC GCCCTGGATT CCTGGGATCC CCAGGACCAC 
CCGGCCTGCC CTCATTCTAC CTATGGAGAA ACACACCTGA AGACTGGGCT GCATTTCAGC 
AAACATCATT CTTCCAGCTT CTTCTTGCAG GCTGGCCTAG AGCACAAGGT CTGCCTGGTC 
CGGAAGGAGA GATGGGAAAA CCTGGGGCAC AGGGGCCACC TGGGGAACCA GGTGAACGAG 
GACCCCCAGG ACGAATGGGA GACATGGGTG ACCGTGGTCC TAAAGGTGTG GTTGGAAGAG 
CGGGAGTTTA TGGAAGAGAT GGAGAGAACG GCCTGGACGG CCCTCAGGGG CCTTCAGGGA 
TTCCAGGCCC AAAGGGTCCT CTGGGTTATA AAGGTGAATC AGGCTCACAT GGAGAGAAAG 
GAGAGGAGGG CATTACTGGA TCAGAGGGTC CATGTGGAGA TAAGGGGAAA CCCGGGTTCA 
AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC GGCCCTCCTG GTCCTCAAGG CATAAGAGGG 
ATGGAGGGCC TTGAAGGGCA TCCAGGACCA GATGGGGAGG ATGGGATGAA TGGATCACCA 
GGTCTTCCAG GTGCACCAGG AGCTCCTGGT TGGACAGGTT TGATTGGTGC CCAGGGGGCT 
AATGGGTCTC GTGGGGAACC TGGGCCTTCT GGAGCTGTTG GACTTCCAGG GCCACAGGGG 
CCCCAGGGAT TAGAAGGACA GATCGGACCT CCAGGACAAA GAGGTCCACC GGGGTTGTCA 
GGCCGAGAGG GTTTGCATGG TCCCAAAGGA GAGCCTGGTC CTGTAGGACC AGTTGGTATC 
AGAGGGGATC CAGGATTTGA AGGATTAAAG GGTGTTTTAG GAACGCCCGG TCCAAAAGGT 
TTCACTGGTA TCAAAGGTAA CAGAGGACCT GATGGAGACC AAGGGGACAT TGGACCTAAA 
GGAAACAAAG GTGCTAGAGG AGCAGCAGGT GTACAGGGCA TCCAAGGTGA ACAGGGACCA 
ATAGGCTTTC CTGGCTTTCC AGGCATCAGA GGCCCACCAG GACCACAGGG AAACCAGGGA 
GAGGATGGAG AGGCTGGTCC ACATGGTAAA GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA 
GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT CCTAGAGGCC TAAAGGGGCG AACAGGGCAG 
AGGGGTCACA CTGGCATGCC TGGACCTCCT GGTTTACCTG GCCCACCTGG ACCATTCGGA 
GCTGAAGGAA AGCCTGGTAC ACAGGGACTG CAAGGCCTTG TGGGTAATGC CGGAAATAAA 
GGATCAACGG GATTGCAAGG TATTCCCGGA GAACGTGGTC AAGACGGACC CCCAGGAACT 
ATGGGCCCAC CTGGACGAGA GGGGCCTATG GGCAGCCCAG GACCACCAGG AGAAAGGGGT 
TTTGGTGGAA AGACAGGAAT GAAGGGACCA CAGGGCCCTG TTGGCATGTA TGGTTTTCCA 
GGAACCGTGG GTATGAGAGG AAGACCAGGC CACACTGGAG ACAGAGGAGA GCCAGGCCCA 
AGAGGCCCTA CTGGACCAAT TGGAAAGACT GGCGTACCAG GTCCCCCTGG TCCAGTAGGA 
GAAAAAGGAC TGCCAGGACC CCAGGGCCGC CAAGGAGATC CTGGGCATCA GGGAACAGAT 
GGCCCATGTG GTCCTCCAGG ACGTCACGGA GTCAGTGGGG TCATTGGAAA ACCAGGACCT 
CGTGGAAGTA TAGGTCCCCC AGGGGAACCT GGGCCAAGTG GGCCCCCAGG AATACCAGGT 
CCACAGGGGG AAAACGGAGT GCCAGGTATA GATGGAGAGA AAGGAGAAAA GGGAATGATG 
GGTTCTCCAG GTGATGATGG ACCTCTTGGA CTGGAAGGAC AACAGGGTCT AACTGGTCCT 
GCAGGTTTGG ATGGGGCTCC TGGAAGGAAG GGTGATAAGG GGGATAGAGG ACCTATCGGA 
ACGTCTGGTC TTCAAGGACC AAAAGGCGAT ATTGGCCCTG TAGGTGATAA AGGTCCACCG 
GGTCCACCAG GCCTAGTAGG CAATGAGGGT GATTCTGGTC CTGCTGGTGT TGCTGGTGAC 
CCCGGGTCAA AAGGAGAAAA GGGTGATATT GGGCCACCAG GATTTACTGG TTTAGAGGGT 
CCATGGGGAG ACACAGGGGA GCAGGGAGAG AAGGGGATCA AGGGAGCCAA GGGACAAATC 
GGAATGCAGG GTGAGCCAGG CCTAGTTGGT GGGGTTGGAC ATCCTGGCCT TAAAGGTGCA 
GAAGGTGTAC CTGGCCATCC AGGACTGGTT GGGCCAGATG GACCTGCAGG AAGCAAGGGA 
AAGACCGGGC CAAGTGGTTC ACCTGGTGAG CCAGGAAATC CTGGACCTGA GGGAATTGCT 
GGTCCCAAAG GTGTGCGAGG TGACACAGGA AAAGGCGGGA CACAGGGTGG TGTGGGTCTA 
GTTGGTCCCA TAGGTCCTTT GGGCCCCAAA GGTCTTTCAG GGCCTGAAGG CGAGAAAGGA 
GAGCCAGGAG ATAAAGGAGA ACCAGGGTTA GAGGGACCAG ATGGCCTTCA GGGTGTACAA 
GGACCACCAG GACTGCCTGG TTTGGAGGGT TCGCAAGGTG AGGCAGGGAT GAGGGGCCCA 
CCAGGTCTGC CAGGAGCTCA GGGCGGCGTG GGTAAGCCAG GACCTGACGG AAGAGAAGGC 
ATGAAAGGAG AGAAGGGGGA TCAAGGTAAA AATGGAGCAC CTGGAAAATT GGGTCACATT 
GGACGAAGGG GTAAAAGAGG CAAGGCAGGT TTGAGGGCGA CCAGAGGACC AAGGGGAGAA 
AAGGGAGAGA AAGGAGATGT TGGTGAGAAA GGACTTCCTG GATGGGGAGG GAGTATGGGA 
ATCCAAGGGC CAAGAGGAGA GCCTGGAGAT GAAGGTGTAA AAGGAGCAGA GGGTGAGAAA 
GGGGATCAGG GACCATTTGG TTCACCTGGG AGAGATGGCA TAAAGGGAAG TCTTGGACCT 
CTTGGCCCTC CTGGACCCCC AGGACCAAAG GGTGATCAGG GAAATATTGG TCCTTCTGGT 
CCTAGAGGGA CACCTGGAAT ACCTGGACTG CCGGGCTTGT TTGGAGACAA GGGACTGAAA 
GGATTTCAAG GGCCTCCTGG ACCCCCTGGA ACAAGGGGTC TGCCAGGTCC ACCAGGGCCA 
CCAGGACCTC CTGGTATCTC TGTGAACCTG ACTCTGATCC AGATCAAGGA CCTGATGTAC 
AGTTCAGACA AGCCCAATTT CTCCCTGATA CAGACACTGC TGGACTCACT GTACCATGAC 
CTCCAGCTGC TGGTGGACCC TCCGGACGGC ACTAAGCAGC ACCCAGCATC AACCTGTCTG 
GAGCTGTGGC TGTGCCATCC CAACTACACG AGTGGTTTCT ACTACATTGA TCCAAACCAA 
GGCAGTCCTT TAGATGCACT TTTAGCCTAC TGTAATTTCT CTGAGTCCGG AGCAGAGACC 
TGTCTTCACC CGAGAGATGC ACATCTGCCC ACAAGGACTT GGCTAAATGA CTCAGGAAAT 
AACAGCAAGT TCCACTGGTT AAGCTCACTA GAGGGGGGTT TTAAATTTGA GTATCCAGCC 
CTAAGTGTGG TTCAGCTGCG CTTTCTGAGA CTCCAGAGCA ACAGAGCGGA GCAGAGAGTG 
ACGTACTCCT GTTACCCTGG ACACAGACTG GGAGAAACAC AGCGACAGGT CCAGTTCCTC 
ACAGACGCCG CCAAGCAGAG CTATTTAGGA GCACTGCAGG ACTGTGTGTC TGCAAAGGAA 
GTGGATCCTG GGCCGCGTGA GTCTGTGTTT CAGTTTGAGG ACATGGACCT GCTTCCTCTA 
CGTGATATAG GGATCTTTGG AAACAGCAGT CAAGAGTTTG GCTTCACCAT TGGCCCTGTA 
TGCTTCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-196i2.1 ( XM_009295265.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293540.1 Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa44245
Clone ID Related Accession (Same CDS sequence)	XM_009295265.1
Accession Version	XM_009295265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(V) chain-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGTGT CAGTAGTGTG GACAGCAGTT TTATTTACTC TGGTAACAGC CTCCATCACG 
GACACCAGTC CTACTGACGT ACCAGACACG GAGAGCGGTT CGGGGATGTT TCTACCAGAT 
GCAATCGACC TTCTTGATCA TCTATGGCAG GTTGCTAATC ACAGTAATGC ATCGGTAACG 
CATGAAAGCA AAAGGTGTCC AGTTCTGCAT GTAGGCCAGT ACTCAACACT TTCTGTCCCA 
CTACGGCAGG TGTTCGGATA CAGTTTTGGT GATGAGTTCA GTTTGCTGTT GCATCTGCGC 
AGCTCTCAGT CTGAAGACCG CAGTGTGTTG ACCTTCCTCT GTCCAGATGG ACACATTCTT 
CTGCAGATCC GCATCAGTGC CTACACTTTT AGCTTCATCA GCACTCAACA GCGACACTAC 
GAATTCCCCG TGGGAGTACT GTCTGATGGA GGTTGGCACC GTGTGGCAGT CAGCGTTTCC 
TCGAGTCGGT TGGCATTGTA TGTGGATTGC TCCATGGTGG AGAGTGTGGA ATGGGCCTAT 
AAGGGCGGTC TGGAAATCTC CACCGATGGC CTGGTTATGT TGGGAGGAAT CATTGAGGGG 
TTTGAGACCC CTTTTGAGGG CGATTTGATG CAAGTGGCAT TTCTGATTGG AGAACCAGAT 
GCAGCTCAAG ATCACTGTGC CTTTTTCCAG CCCCAGTGTG GTGGAACTGG ACACAAACCA 
CCTCGCTCTC CTCGCATACA CACTGAAGAG GAACTTTTGC TATCCTCTAA TGACCTGGAG 
GACTTGTTAG AGTCAGCAAA GAACAGTGAC TCTATTCGGG CAAAACTGAA TGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGTAC ACAGGGACTG 
CAAGGCCTTG TGGGTAATGC CGGAAATAAA GGATCAACGG GATTGCAAGG TATTCCCGGA 
GAACGTGGTC AAGACGGACC CCCAGGAACT ATGGGCCCAC CTGGACGAGA GGGGCCTATG 
GGCAGCCCAG GACCACCAGG AGAAAGGGGT TTTGGTGGAA AGACAGGAAT GAAGGGACCA 
CAGGGCCCTG TTGGCATGGT TTTCCAGGAA CCGTGGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293540.1
CDS	167..3208
Translation	MLVSVVWTAVLFTLVTASITDTSPTDVPDTESGSGMFLPDAIDLLDHLWQVANHSNASVTHESKRCPVLHVGQYSTLSVPLRQVFGYSFGDEFSLLLHLRSSQSEDRSVLTFLCPDGHILLQIRISAYTFSFISTQQRHYEFPVGVLSDGGWHRVAVSVSSSRLALYVDCSMVESVEWAYKGGLEISTDGLVMLGGIIEGFETPFEGDLMQVAFLIGEPDAAQDHCAFFQPQCGGTGHKPPRSPRIHTEEELLLSSNDLEDLLESAKNSDSIRAKLNGLRRRGIIRGDGTLPTGSKRPAIVGRGDVFEVDEDTDLLDQNILSKNGDKPSKTFPTDTTSSRDGKLESTSKHLDENITIDKHKPGKPLPKKPVDTTIINLDVSEKPTGSTVPSREIHLMTTSSPEDGESNLLKEDFEVTTQAPGRTSWDNVSKGSGRTPSKSPDIHAGSGGGSEEHPDTIVLTGRQGDIVRGSDGKMYRIQRGPMGPMGPPGEPGCPGNAGYPGPKGDKGAFGVRGRPGRPGFLGSPGPPGLPSFYLWRNTPEDWAAFQQTSFFQLLLAGWPRAQGLPGPEGEMGKPGAQGPPGEPGERGPPGRMGDMGDRGPKGVVGRAGVYGRDGENGLDGPQGPSGIPGPKGPLGYKGESGSHGEKGEEGITGSEGPCGDKGKPGEKGSKGAPGAPGAVGPPGPQGIRGMEGLEGHPGPDGEDGMNGSPGLPGAPGAPGWTGLIGAQGANGSRGEPGPSGAVGLPGPQGPQGLEGQIGPPGQRGPPGLSGREGLHGPKGEPGPVGPVGIRGDPGFEGLKGVLGTPGPKGFTGIKGNRGPDGDQGDIGPKGNKGARGAAGVQGIQGEQGPIGFPGFPGIRGPPGPQGNQGEDGEAGPHGKVGKEGSTGSRGPEGLSGKPGPRGLKGRTGQRGHTGMPGPPGLPGPPGPFGAEGKPGTQGLQGLVGNAGNKGSTGLQGIPGERGQDGPPGTMGPPGREGPMGSPGPPGERGFGGKTGMKGPQGPVGMVFQEPWV

Target ORF information:

RefSeq Version	XM_009295265.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295265.1

ORF Insert Sequence:

ATGCTCGTGT CAGTAGTGTG GACAGCAGTT TTATTTACTC TGGTAACAGC CTCCATCACG 
GACACCAGTC CTACTGACGT ACCAGACACG GAGAGCGGTT CGGGGATGTT TCTACCAGAT 
GCAATCGACC TTCTTGATCA TCTATGGCAG GTTGCTAATC ACAGTAATGC ATCGGTAACG 
CATGAAAGCA AAAGGTGTCC AGTTCTGCAT GTAGGCCAGT ACTCAACACT TTCTGTCCCA 
CTACGGCAGG TGTTCGGATA CAGTTTTGGT GATGAGTTCA GTTTGCTGTT GCATCTGCGC 
AGCTCTCAGT CTGAAGACCG CAGTGTGTTG ACCTTCCTCT GTCCAGATGG ACACATTCTT 
CTGCAGATCC GCATCAGTGC CTACACTTTT AGCTTCATCA GCACTCAACA GCGACACTAC 
GAATTCCCCG TGGGAGTACT GTCTGATGGA GGTTGGCACC GTGTGGCAGT CAGCGTTTCC 
TCGAGTCGGT TGGCATTGTA TGTGGATTGC TCCATGGTGG AGAGTGTGGA ATGGGCCTAT 
AAGGGCGGTC TGGAAATCTC CACCGATGGC CTGGTTATGT TGGGAGGAAT CATTGAGGGG 
TTTGAGACCC CTTTTGAGGG CGATTTGATG CAAGTGGCAT TTCTGATTGG AGAACCAGAT 
GCAGCTCAAG ATCACTGTGC CTTTTTCCAG CCCCAGTGTG GTGGAACTGG ACACAAACCA 
CCTCGCTCTC CTCGCATACA CACTGAAGAG GAACTTTTGC TATCCTCTAA TGACCTGGAG 
GACTTGTTAG AGTCAGCAAA GAACAGTGAC TCTATTCGGG CAAAACTGAA TGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGTAC ACAGGGACTG 
CAAGGCCTTG TGGGTAATGC CGGAAATAAA GGATCAACGG GATTGCAAGG TATTCCCGGA 
GAACGTGGTC AAGACGGACC CCCAGGAACT ATGGGCCCAC CTGGACGAGA GGGGCCTATG 
GGCAGCCCAG GACCACCAGG AGAAAGGGGT TTTGGTGGAA AGACAGGAAT GAAGGGACCA 
CAGGGCCCTG TTGGCATGGT TTTCCAGGAA CCGTGGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-196i2.1 ( XM_021473390.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329065.1 Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa146092
Clone ID Related Accession (Same CDS sequence)	XM_021473390.1
Accession Version	XM_021473390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(I) chain-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395079.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGACACCA TGCTGCCCTC AGGATATGGA GATGCAATCG ACCTTCTTGA TCATCTGTGG 
CAGGTTGCTA ATCACAGTAA TGCATCGGTA ACGCAGGAAA GCAAAAGGTG TCCAGTTCTG 
CATGTAGGCC AGTACTCAAC ACTTTCTGTC CCACTACGGC AGGTGTTCGG ATACAGTTTT 
GGTGATGAGT TCAGTTTGCT GTTGCATCTG CGCAGCTCTC AGTCTGAAGA CCGCAGTGTG 
TTGACCTTCC TCTGTCCAGA TGGACACATT CTTCTGCAGA TCCGCATCAG TGCCTACACT 
TTTAGCTTCA TCAGCACTCA ACAGCGACAC TACGAATTCC CCGTGGGAGT ACTGTCTGAT 
GGAGGTTGGC ACCGTGTGGC AGTCAGCGTT TCCTCGAGTC GGTTGGCATT GTATGTGGAT 
TGCTCCATGG TGGAGAGTGT GGAATGGGCC TATAAGGGCG GTCTGGAAAT CTCCACCGAT 
GGCCTGGTTA TGTTGGGAGG AATCATTGAG GGGTTTGAGA CCCCTTTTGA GGGCGATTTG 
ATGCAAGTGG CATTTCTGAT TGGAGAACCA GATGCAGCTC AAGATCACTG TGCCTTTTTC 
CAGCCCCAGT GTGGTGGAAC TGGACACAAA CCACCTCGCT CTCCTCGCAT ACACACTGAA 
GAGGAACTGT TGCTATCCTC AAATGACCTG GAGGACTTGT TAGAGTCAGC AAAGAACAGT 
GACTCTATTC AGGCAAAACT GAATGGTCTT CGTAGACGTG GGATTATTCG TGGGGATGGG 
ACTTTGCCCA CTGGATCCAA GCGTCCTGCA ATTGTGGGCC GTGGAGATGT GTTTGAGGTG 
GATGAGGATA CAGACCTTCT GGACCAAAAC ATCTTGTCTA AAAATGGAGA TAAACCTTCC 
AAAACCTTCC CAACTGACAC TACAAGCAGC CGTGATGGTA AGCTAGAATC CACATCTAAA 
CATCTTGATG AAAACATCAC CGTTGACAAG CACAAACCTG GCAAACCTTT ACAAAAGAAG 
CCAGTGGACA CTACAATTAT CAATCTGGAT GTATCTGAGA AACCTACCGG AAGTACTGTT 
CCTAGTAGAG AAATCCACTT GATGACCACA TCCAGTCCTG AGGATGGAGA ATCCAACTTG 
TTAAAAGAGG ACTTCGAGGT CACCACACAG GCTCCTGGTA GGACTTCTTG GGACAATGTG 
TCAAAAGGCA GTGGAAGGAC ACCATCGAAA TCTCCAGATA TTCATGCAGG ATCTGGAGGG 
GGATCTGAGG AGCATCCGGA TACCATTGTG CTTACAGGCA GACAAGGAGA CATTGTGCGT 
GGGTCTGATG GGAAAATGTA TCGCATCCAA AGAGGGCCTA TGGGGCCAAT GGGGCCCCCA 
GGTGAGCCTG GGTGTCCTGG GAATCCTGGC TATCCTGGAC CCAAAGGAGA TAAGGGTGCC 
TTTGGTGTTA GGGGTCGTCC AGGTCGCCCT GGATTCCTGG GACCCCCAGG ACCACCCGGC 
CTGCCCACAT TCTACCTATG GAGAAACACA CCTGAAGACT GGGCTGCATT TCAGCAAACA 
TCATTCTTCC AGCTTCTTCT TGCAGGCTGG CCTAGAGCAC AAGGTCTGCC TGGTCCGGAA 
GGAGAGATGG GAAAACCTGG GGCACAGGGG CCACCTGGGG AACCAGGTGA ACGAGGACCC 
CCAGGACGAA TGGGAGACAT GGGTGACCGT GGTCCTAAAG GTGTGGTTGG AAGAGCAGGA 
GTTTATGGAA GAGATGGAGA GAACGGCCTG GACGGCCCTC AGGGGCCTTC AGGGATTCCA 
GGCCCAAAGG GTCCTCTGGG TTATAAAGGT GAATCAGGCT CACATGGAGA GAAAGGAGAG 
GAGGGCATTG CTGGATCAGA GGGTCCATGT GGAGATAAGG GGAAACCCGG TGAGAAAGGT 
TCAAAAGGAG CACCTGGCGC TCCTGGGGCT GTCGGCCCTC CTGGTCCTCA AGGCATAAGA 
GGGATGGAGG GCCTTGAAGG GCATCCAGGA CCAGATGGGG AGGATGGGAT GAATGGATCA 
CCAGGTCTTC CAGGTGCACC AGGAGCTCCT GGTTGGACAG GTTTGATTGG TGCCCAGGGG 
GCTAATGGGT CTCGTGGGGA ACCTGGGCCT TCTGGAGCTG TTGGACTTCC AGGGCCACAG 
GGGCCCCAGG GATTAGAAGG ACAGATCGGA CCTCCAGGAC AAAGAGGTCC ACCGGGGTTG 
TCAGGCCGAG AGGGTTTGCA TGGTCCCAAA GGAGAGCCTG GTCCTGTAGG ACCAGTTGGT 
ATCAGAGGGG ATCCAGGATT TGAAGGATTA AAGGGTGTTT TAGGAACGCC CGGTCCAAAA 
GGTTTCACTG GTATCAAAGG TAACAGAGGA CCTGATGGAG ACCAAGGGGA CATTGGACCT 
AAAGGAAACA AAGGTGCTAG AGGAGCAGCA GGTGTACAGG GCATCCAAGG TGAACAGGGA 
CCAATAGGCT TTCCTGGCTT TCCAGGCATC AGAGGCCCAC CAGGACCACA GGGAAACCAG 
GGAGAGGATG GAGAGGCTGG TCCACATGGT AAAGTGGGCA AAGAGGGGTC AAAGGGGAGC 
AGAGGTCCTG AAGGCCTCTC TGGAAAGCCT GGTCCTAGAG GCCTAAAGGG GCGAACAGGG 
CAGAGGGGTC ACACTGGCAT GCCTGGACCT CCTGGTTTAC CTGGCCCACC TGGACCATTC 
GGAGCTGAAG GAAAGCCTGG TACACAGGGA CTGCAAGGCC TTGTGGGTAA TGCCGGAAAT 
AAAGGATCAA CGGGATTGCA AGGTATTCCC GGAGAACGTG GTCAAGACGG ACCCCCAGGA 
ACTATGGGCC CACCTGGACG AGAGGGGCCT ATGGGCAGCC CAGGACCACC AGGAGAAAGG 
GGTTTTGGTG GAAAGACAGG AATGGAGGGA CCACAGGGCC CTGTTGGCAT GTATGGTTTT 
CCAGGAACCG TGGGTATGAG AGGAAGACCA GGCCACACTG GAGACAGAGG AGAGCCAGGC 
CCAAGAGGCC CTACTGGACC AATTGGAAAG ACTGGTGTAC CAGGTCCCCC TGGTCCAGTA 
GGAGAAAAAG GACTGCCAGG ACCCCAGGGC CGCCAAGGAG ATCCTGGGCA TCAGGGAACA 
GATGGCCCAT GTGGTCCTCC AGGACGTCAG GGAGTCAGTG GGGTCATTGG AAAACCAGGA 
CCTCGTGGAA GTATAGGTCC CCCAGGGGAA CCTGGGCCAA GTGGGCCCCC AGGAATACCA 
GGTCCACAGG GGGAAAATGG AGTGCCAGGT ATAGATGGAG AGAAAGGAGA AAAGGGAATG 
ATGGGTTCTC CTGGTGATGA TGGACCTCTT GGACTGGAAG GACAACAGGG TCTAACTGGT 
CCTGCAGGTT TGGATGGGGC TCCTGGAAGG AAGGGTGATA AGGGGGATAG AGGACCTATC 
GGAACATCTG GTCTTCAAGG ACCAAAAGGC GATACTGGTC CTGTAGGTGA TAAAGGTCCA 
CCGGGTCCAC CAGGTCTAGT AGGCAATGAG GGTGATTCTG GTCCTGCTGG TGTTGCTGGT 
GACCCCGGGT CAAAAGGAGA AAAGGGTGAA ATTGGGCCAC CAGGATTTAC TGGTTTAGAG 
GGTCCATGGG GAGACACAGG GGAGCAGGGA GAGAAGGGGA TCAAGGGAGC CAAGGGACAA 
ATCGGAATGC AGGGTGAGCC AGGACTAGTT GGTGGGGTTG GACATCCTGG CCTTAAAGGT 
GCAGAAGGTG TACCTGGCCA TCCAGGACTG GTTGGGCCAG ATGGACCTGC AGGAAGCAAG 
GGAAAGACCG GGCCAAGTGG TTCACCTGGT GAGCCAGGAA ATCCTGGACC TGAGGGAATT 
GCTGGTCCCA AAGGTGTGCG AGGTGACACA GGAAAAGGCG GGACACAGGG TGGTGTGGGT 
CTAGTTGGTC CCATAGGTCC TTTGGGCCCC AAAGGTCTTT CAGGGCCTGA AGGCGAGAAA 
GGAGAGCCAG GAGATAAAGG AGAACCAGGG TTAGAGGGAC CAGATGGCCT TCAGGGTGTA 
CAAGGACCAC CAGGATTGCC TGGTTTGGAG GGTTCACAAG GTGAGGCAGG GATAAGGGGC 
CCACCAGGTC TGCCAGGAGC TCAGGGCGGC GTGGGTAAGC CAGGACCTGA CGGAAGAGAA 
GGCATGAAAG GAGAGAAGGG GGATCAAGGT AAAAATGGAG CACCTGGAAA ATTGGGTCAC 
ATTGGACGAA GGGGTAAAAG AGGCAAGGCA GGTTTGAGGG CGACCAGAGG ACCAAGGGGA 
GAAAAGGGAG AGAAAGGAGA TGTTGGTGAG AAAGGACTTC CTGGATGGGG AGGGAGTATG 
GGAATCCAAG GGCCAAGAGG AGAGCCTGGA GATGAAGGTG TAAAAGGAGC AGAGGGTGAG 
AAAGGGGATC AGGGACCATT TGGTTCACCT GGGAGAGATG GCATAAAGGG AAGTCTTGGA 
CCTCTTGGCC CTCCTGGACC CCCAGGACCA AAGGGTGATC AGGGAAATAT TGGTCCTTCT 
GGTCCTAGAG GGACACCTGG AATACCTGGA CTGCCGGGCT TGTTTGGAGA CAAGGGACTG 
AAAGGATTTC AAGGGCCTCC TGGACCCCCT GGAACAAGGG GTCTGCCAGG TCCACCAGGG 
CCACCAGGAC CTCCTGGTAT CTCTGTGAAC CTGACTCTGA TCCAGATCAA GGACCTGATG 
TACAGTTCAG ACAAGCCCAA TTTCTCCCTG ATTCAGACAC TGCTGGACTC ACTGTACCAT 
GACCTCCAGC TGCTGGTGGA GCCTCCGGAC GGCACTAAGC AGCACCCAGC ATCAACCTGT 
CTGGAGCTGT GGCTGTGCCA TCCCAACTAC ACGAGTGGTT TCTACTACAT TGATCCAAAC 
CAAGGCAGTC CTTTAGATGC ACTTTTAGCC TACTGTAATT TCTCAGAGTC CGGAGCAGAG 
ACCTGTCTTC ACCCTAGAGA TGCACATCTG CCCACAAGGA CTTGGCTAAA TGACTCAGGA 
AATAACAGCA AGTTCCACTG GTTAAGCTCA CTAGAGGGGG GTTTTAAATT TGAGTATCCA 
GCCCTAAGTG TGGTTCAGCT GCGCTTTCTG AGACTCCAGA GCAACAGAGC GGAGCAGAGA 
GTGACGTACT CCTGTTACCC TGGACACAGA CTGGGAGAAA TACAGCGACA GGTCCAGTTC 
CTCACAGACG CCGCCAAGCA GAGCTATTTA GGAGCACTGC AGGACTGTGT GTCTGCAAAG 
GAAGTGGATC CTGGGCCGCG TGAGTCTGTG TTTCAGTTTG AGGACATGGA CCTGCTTCCT 
CTACGTGATA TAGGGATCTT TGGAAACAGC AGTCAAGAGT TTGGCTTCAC CATTGGCCCT 
GTATGCTTCA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329065.1
CDS	1..5355
Translation	MDTMLPSGYGDAIDLLDHLWQVANHSNASVTQESKRCPVLHVGQYSTLSVPLRQVFGYSFGDEFSLLLHLRSSQSEDRSVLTFLCPDGHILLQIRISAYTFSFISTQQRHYEFPVGVLSDGGWHRVAVSVSSSRLALYVDCSMVESVEWAYKGGLEISTDGLVMLGGIIEGFETPFEGDLMQVAFLIGEPDAAQDHCAFFQPQCGGTGHKPPRSPRIHTEEELLLSSNDLEDLLESAKNSDSIQAKLNGLRRRGIIRGDGTLPTGSKRPAIVGRGDVFEVDEDTDLLDQNILSKNGDKPSKTFPTDTTSSRDGKLESTSKHLDENITVDKHKPGKPLQKKPVDTTIINLDVSEKPTGSTVPSREIHLMTTSSPEDGESNLLKEDFEVTTQAPGRTSWDNVSKGSGRTPSKSPDIHAGSGGGSEEHPDTIVLTGRQGDIVRGSDGKMYRIQRGPMGPMGPPGEPGCPGNPGYPGPKGDKGAFGVRGRPGRPGFLGPPGPPGLPTFYLWRNTPEDWAAFQQTSFFQLLLAGWPRAQGLPGPEGEMGKPGAQGPPGEPGERGPPGRMGDMGDRGPKGVVGRAGVYGRDGENGLDGPQGPSGIPGPKGPLGYKGESGSHGEKGEEGIAGSEGPCGDKGKPGEKGSKGAPGAPGAVGPPGPQGIRGMEGLEGHPGPDGEDGMNGSPGLPGAPGAPGWTGLIGAQGANGSRGEPGPSGAVGLPGPQGPQGLEGQIGPPGQRGPPGLSGREGLHGPKGEPGPVGPVGIRGDPGFEGLKGVLGTPGPKGFTGIKGNRGPDGDQGDIGPKGNKGARGAAGVQGIQGEQGPIGFPGFPGIRGPPGPQGNQGEDGEAGPHGKVGKEGSKGSRGPEGLSGKPGPRGLKGRTGQRGHTGMPGPPGLPGPPGPFGAEGKPGTQGLQGLVGNAGNKGSTGLQGIPGERGQDGPPGTMGPPGREGPMGSPGPPGERGFGGKTGMEGPQGPVGMYGFPGTVGMRGRPGHTGDRGEPGPRGPTGPIGKTGVPGPPGPVGEKGLPGPQGRQGDPGHQGTDGPCGPPGRQGVSGVIGKPGPRGSIGPPGEPGPSGPPGIPGPQGENGVPGIDGEKGEKGMMGSPGDDGPLGLEGQQGLTGPAGLDGAPGRKGDKGDRGPIGTSGLQGPKGDTGPVGDKGPPGPPGLVGNEGDSGPAGVAGDPGSKGEKGEIGPPGFTGLEGPWGDTGEQGEKGIKGAKGQIGMQGEPGLVGGVGHPGLKGAEGVPGHPGLVGPDGPAGSKGKTGPSGSPGEPGNPGPEGIAGPKGVRGDTGKGGTQGGVGLVGPIGPLGPKGLSGPEGEKGEPGDKGEPGLEGPDGLQGVQGPPGLPGLEGSQGEAGIRGPPGLPGAQGGVGKPGPDGREGMKGEKGDQGKNGAPGKLGHIGRRGKRGKAGLRATRGPRGEKGEKGDVGEKGLPGWGGSMGIQGPRGEPGDEGVKGAEGEKGDQGPFGSPGRDGIKGSLGPLGPPGPPGPKGDQGNIGPSGPRGTPGIPGLPGLFGDKGLKGFQGPPGPPGTRGLPGPPGPPGPPGISVNLTLIQIKDLMYSSDKPNFSLIQTLLDSLYHDLQLLVEPPDGTKQHPASTCLELWLCHPNYTSGFYYIDPNQGSPLDALLAYCNFSESGAETCLHPRDAHLPTRTWLNDSGNNSKFHWLSSLEGGFKFEYPALSVVQLRFLRLQSNRAEQRVTYSCYPGHRLGEIQRQVQFLTDAAKQSYLGALQDCVSAKEVDPGPRESVFQFEDMDLLPLRDIGIFGNSSQEFGFTIGPVCFS

Target ORF information:

RefSeq Version	XM_021473390.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473390.1

ORF Insert Sequence:

ATGGACACCA TGCTGCCCTC AGGATATGGA GATGCAATCG ACCTTCTTGA TCATCTGTGG 
CAGGTTGCTA ATCACAGTAA TGCATCGGTA ACGCAGGAAA GCAAAAGGTG TCCAGTTCTG 
CATGTAGGCC AGTACTCAAC ACTTTCTGTC CCACTACGGC AGGTGTTCGG ATACAGTTTT 
GGTGATGAGT TCAGTTTGCT GTTGCATCTG CGCAGCTCTC AGTCTGAAGA CCGCAGTGTG 
TTGACCTTCC TCTGTCCAGA TGGACACATT CTTCTGCAGA TCCGCATCAG TGCCTACACT 
TTTAGCTTCA TCAGCACTCA ACAGCGACAC TACGAATTCC CCGTGGGAGT ACTGTCTGAT 
GGAGGTTGGC ACCGTGTGGC AGTCAGCGTT TCCTCGAGTC GGTTGGCATT GTATGTGGAT 
TGCTCCATGG TGGAGAGTGT GGAATGGGCC TATAAGGGCG GTCTGGAAAT CTCCACCGAT 
GGCCTGGTTA TGTTGGGAGG AATCATTGAG GGGTTTGAGA CCCCTTTTGA GGGCGATTTG 
ATGCAAGTGG CATTTCTGAT TGGAGAACCA GATGCAGCTC AAGATCACTG TGCCTTTTTC 
CAGCCCCAGT GTGGTGGAAC TGGACACAAA CCACCTCGCT CTCCTCGCAT ACACACTGAA 
GAGGAACTGT TGCTATCCTC AAATGACCTG GAGGACTTGT TAGAGTCAGC AAAGAACAGT 
GACTCTATTC AGGCAAAACT GAATGGTCTT CGTAGACGTG GGATTATTCG TGGGGATGGG 
ACTTTGCCCA CTGGATCCAA GCGTCCTGCA ATTGTGGGCC GTGGAGATGT GTTTGAGGTG 
GATGAGGATA CAGACCTTCT GGACCAAAAC ATCTTGTCTA AAAATGGAGA TAAACCTTCC 
AAAACCTTCC CAACTGACAC TACAAGCAGC CGTGATGGTA AGCTAGAATC CACATCTAAA 
CATCTTGATG AAAACATCAC CGTTGACAAG CACAAACCTG GCAAACCTTT ACAAAAGAAG 
CCAGTGGACA CTACAATTAT CAATCTGGAT GTATCTGAGA AACCTACCGG AAGTACTGTT 
CCTAGTAGAG AAATCCACTT GATGACCACA TCCAGTCCTG AGGATGGAGA ATCCAACTTG 
TTAAAAGAGG ACTTCGAGGT CACCACACAG GCTCCTGGTA GGACTTCTTG GGACAATGTG 
TCAAAAGGCA GTGGAAGGAC ACCATCGAAA TCTCCAGATA TTCATGCAGG ATCTGGAGGG 
GGATCTGAGG AGCATCCGGA TACCATTGTG CTTACAGGCA GACAAGGAGA CATTGTGCGT 
GGGTCTGATG GGAAAATGTA TCGCATCCAA AGAGGGCCTA TGGGGCCAAT GGGGCCCCCA 
GGTGAGCCTG GGTGTCCTGG GAATCCTGGC TATCCTGGAC CCAAAGGAGA TAAGGGTGCC 
TTTGGTGTTA GGGGTCGTCC AGGTCGCCCT GGATTCCTGG GACCCCCAGG ACCACCCGGC 
CTGCCCACAT TCTACCTATG GAGAAACACA CCTGAAGACT GGGCTGCATT TCAGCAAACA 
TCATTCTTCC AGCTTCTTCT TGCAGGCTGG CCTAGAGCAC AAGGTCTGCC TGGTCCGGAA 
GGAGAGATGG GAAAACCTGG GGCACAGGGG CCACCTGGGG AACCAGGTGA ACGAGGACCC 
CCAGGACGAA TGGGAGACAT GGGTGACCGT GGTCCTAAAG GTGTGGTTGG AAGAGCAGGA 
GTTTATGGAA GAGATGGAGA GAACGGCCTG GACGGCCCTC AGGGGCCTTC AGGGATTCCA 
GGCCCAAAGG GTCCTCTGGG TTATAAAGGT GAATCAGGCT CACATGGAGA GAAAGGAGAG 
GAGGGCATTG CTGGATCAGA GGGTCCATGT GGAGATAAGG GGAAACCCGG TGAGAAAGGT 
TCAAAAGGAG CACCTGGCGC TCCTGGGGCT GTCGGCCCTC CTGGTCCTCA AGGCATAAGA 
GGGATGGAGG GCCTTGAAGG GCATCCAGGA CCAGATGGGG AGGATGGGAT GAATGGATCA 
CCAGGTCTTC CAGGTGCACC AGGAGCTCCT GGTTGGACAG GTTTGATTGG TGCCCAGGGG 
GCTAATGGGT CTCGTGGGGA ACCTGGGCCT TCTGGAGCTG TTGGACTTCC AGGGCCACAG 
GGGCCCCAGG GATTAGAAGG ACAGATCGGA CCTCCAGGAC AAAGAGGTCC ACCGGGGTTG 
TCAGGCCGAG AGGGTTTGCA TGGTCCCAAA GGAGAGCCTG GTCCTGTAGG ACCAGTTGGT 
ATCAGAGGGG ATCCAGGATT TGAAGGATTA AAGGGTGTTT TAGGAACGCC CGGTCCAAAA 
GGTTTCACTG GTATCAAAGG TAACAGAGGA CCTGATGGAG ACCAAGGGGA CATTGGACCT 
AAAGGAAACA AAGGTGCTAG AGGAGCAGCA GGTGTACAGG GCATCCAAGG TGAACAGGGA 
CCAATAGGCT TTCCTGGCTT TCCAGGCATC AGAGGCCCAC CAGGACCACA GGGAAACCAG 
GGAGAGGATG GAGAGGCTGG TCCACATGGT AAAGTGGGCA AAGAGGGGTC AAAGGGGAGC 
AGAGGTCCTG AAGGCCTCTC TGGAAAGCCT GGTCCTAGAG GCCTAAAGGG GCGAACAGGG 
CAGAGGGGTC ACACTGGCAT GCCTGGACCT CCTGGTTTAC CTGGCCCACC TGGACCATTC 
GGAGCTGAAG GAAAGCCTGG TACACAGGGA CTGCAAGGCC TTGTGGGTAA TGCCGGAAAT 
AAAGGATCAA CGGGATTGCA AGGTATTCCC GGAGAACGTG GTCAAGACGG ACCCCCAGGA 
ACTATGGGCC CACCTGGACG AGAGGGGCCT ATGGGCAGCC CAGGACCACC AGGAGAAAGG 
GGTTTTGGTG GAAAGACAGG AATGGAGGGA CCACAGGGCC CTGTTGGCAT GTATGGTTTT 
CCAGGAACCG TGGGTATGAG AGGAAGACCA GGCCACACTG GAGACAGAGG AGAGCCAGGC 
CCAAGAGGCC CTACTGGACC AATTGGAAAG ACTGGTGTAC CAGGTCCCCC TGGTCCAGTA 
GGAGAAAAAG GACTGCCAGG ACCCCAGGGC CGCCAAGGAG ATCCTGGGCA TCAGGGAACA 
GATGGCCCAT GTGGTCCTCC AGGACGTCAG GGAGTCAGTG GGGTCATTGG AAAACCAGGA 
CCTCGTGGAA GTATAGGTCC CCCAGGGGAA CCTGGGCCAA GTGGGCCCCC AGGAATACCA 
GGTCCACAGG GGGAAAATGG AGTGCCAGGT ATAGATGGAG AGAAAGGAGA AAAGGGAATG 
ATGGGTTCTC CTGGTGATGA TGGACCTCTT GGACTGGAAG GACAACAGGG TCTAACTGGT 
CCTGCAGGTT TGGATGGGGC TCCTGGAAGG AAGGGTGATA AGGGGGATAG AGGACCTATC 
GGAACATCTG GTCTTCAAGG ACCAAAAGGC GATACTGGTC CTGTAGGTGA TAAAGGTCCA 
CCGGGTCCAC CAGGTCTAGT AGGCAATGAG GGTGATTCTG GTCCTGCTGG TGTTGCTGGT 
GACCCCGGGT CAAAAGGAGA AAAGGGTGAA ATTGGGCCAC CAGGATTTAC TGGTTTAGAG 
GGTCCATGGG GAGACACAGG GGAGCAGGGA GAGAAGGGGA TCAAGGGAGC CAAGGGACAA 
ATCGGAATGC AGGGTGAGCC AGGACTAGTT GGTGGGGTTG GACATCCTGG CCTTAAAGGT 
GCAGAAGGTG TACCTGGCCA TCCAGGACTG GTTGGGCCAG ATGGACCTGC AGGAAGCAAG 
GGAAAGACCG GGCCAAGTGG TTCACCTGGT GAGCCAGGAA ATCCTGGACC TGAGGGAATT 
GCTGGTCCCA AAGGTGTGCG AGGTGACACA GGAAAAGGCG GGACACAGGG TGGTGTGGGT 
CTAGTTGGTC CCATAGGTCC TTTGGGCCCC AAAGGTCTTT CAGGGCCTGA AGGCGAGAAA 
GGAGAGCCAG GAGATAAAGG AGAACCAGGG TTAGAGGGAC CAGATGGCCT TCAGGGTGTA 
CAAGGACCAC CAGGATTGCC TGGTTTGGAG GGTTCACAAG GTGAGGCAGG GATAAGGGGC 
CCACCAGGTC TGCCAGGAGC TCAGGGCGGC GTGGGTAAGC CAGGACCTGA CGGAAGAGAA 
GGCATGAAAG GAGAGAAGGG GGATCAAGGT AAAAATGGAG CACCTGGAAA ATTGGGTCAC 
ATTGGACGAA GGGGTAAAAG AGGCAAGGCA GGTTTGAGGG CGACCAGAGG ACCAAGGGGA 
GAAAAGGGAG AGAAAGGAGA TGTTGGTGAG AAAGGACTTC CTGGATGGGG AGGGAGTATG 
GGAATCCAAG GGCCAAGAGG AGAGCCTGGA GATGAAGGTG TAAAAGGAGC AGAGGGTGAG 
AAAGGGGATC AGGGACCATT TGGTTCACCT GGGAGAGATG GCATAAAGGG AAGTCTTGGA 
CCTCTTGGCC CTCCTGGACC CCCAGGACCA AAGGGTGATC AGGGAAATAT TGGTCCTTCT 
GGTCCTAGAG GGACACCTGG AATACCTGGA CTGCCGGGCT TGTTTGGAGA CAAGGGACTG 
AAAGGATTTC AAGGGCCTCC TGGACCCCCT GGAACAAGGG GTCTGCCAGG TCCACCAGGG 
CCACCAGGAC CTCCTGGTAT CTCTGTGAAC CTGACTCTGA TCCAGATCAA GGACCTGATG 
TACAGTTCAG ACAAGCCCAA TTTCTCCCTG ATTCAGACAC TGCTGGACTC ACTGTACCAT 
GACCTCCAGC TGCTGGTGGA GCCTCCGGAC GGCACTAAGC AGCACCCAGC ATCAACCTGT 
CTGGAGCTGT GGCTGTGCCA TCCCAACTAC ACGAGTGGTT TCTACTACAT TGATCCAAAC 
CAAGGCAGTC CTTTAGATGC ACTTTTAGCC TACTGTAATT TCTCAGAGTC CGGAGCAGAG 
ACCTGTCTTC ACCCTAGAGA TGCACATCTG CCCACAAGGA CTTGGCTAAA TGACTCAGGA 
AATAACAGCA AGTTCCACTG GTTAAGCTCA CTAGAGGGGG GTTTTAAATT TGAGTATCCA 
GCCCTAAGTG TGGTTCAGCT GCGCTTTCTG AGACTCCAGA GCAACAGAGC GGAGCAGAGA 
GTGACGTACT CCTGTTACCC TGGACACAGA CTGGGAGAAA TACAGCGACA GGTCCAGTTC 
CTCACAGACG CCGCCAAGCA GAGCTATTTA GGAGCACTGC AGGACTGTGT GTCTGCAAAG 
GAAGTGGATC CTGGGCCGCG TGAGTCTGTG TTTCAGTTTG AGGACATGGA CCTGCTTCCT 
CTACGTGATA TAGGGATCTT TGGAAACAGC AGTCAAGAGT TTGGCTTCAC CATTGGCCCT 
GTATGCTTCA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-196i2.1 ( XM_009295261.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293536.1 Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X1, mRNA.

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CloneID	ODa44242
Clone ID Related Accession (Same CDS sequence)	XM_009295261.3 , XM_009295261.2
Accession Version	XM_009295261.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(I) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGTGT CAGTAGTGTG GACAGCAGTT TTATTTACTC TGGTAACAGC CTCCATCACG 
GACACCAGTC CTACTGACGT ACCAGACACG GAGAGCGGTT CGGGGATGTT TCTACCAGAT 
GCAATCGACC TTCTTGATCA TCTATGGCAG GTTGCTAATC ACAGTAATGC ATCGGTAACG 
CATGAAAGCA AAAGGTGTCC AGTTCTGCAT GTAGGCCAGT ACTCAACACT TTCTGTCCCA 
CTACGGCAGG TGTTCGGATA CAGTTTTGGT GATGAGTTCA GTTTGCTGTT GCATCTGCGC 
AGCTCTCAGT CTGAAGACCG CAGTGTGTTG ACCTTCCTCT GTCCAGATGG ACACATTCTT 
CTGCAGATCC GCATCAGTGC CTACACTTTT AGCTTCATCA GCACTCAACA GCGACACTAC 
GAATTCCCCG TGGGAGTACT GTCTGATGGA GGTTGGCACC GTGTGGCAGT CAGCGTTTCC 
TCGAGTCGGT TGGCATTGTA TGTGGATTGC TCCATGGTGG AGAGTGTGGA ATGGGCCTAT 
AAGGGCGGTC TGGAAATCTC CACCGATGGC CTGGTTATGT TGGGAGGAAT CATTGAGGGG 
TTTGAGACCC CTTTTGAGGG CGATTTGATG CAAGTGGCAT TTCTGATTGG AGAACCAGAT 
GCAGCTCAAG ATCACTGTGC CTTTTTCCAG CCCCAGTGTG GTGGAACTGG ACACAAACCA 
CCTCGCTCTC CTCGCATACA CACTGAAGAG GAACTTTTGC TATCCTCTAA TGACCTGGAG 
GACTTGTTAG AGTCAGCAAA GAACAGTGAC TCTATTCGGG CAAAACTGAA TGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGTAC ACAGGGACTG 
CAAGGCCTTG TGGGTAATGC CGGAAATAAA GGATCAACGG GATTGCAAGG TATTCCCGGA 
GAACGTGGTC AAGACGGACC CCCAGGAACT ATGGGCCCAC CTGGACGAGA GGGGCCTATG 
GGCAGCCCAG GACCACCAGG AGAAAGGGGT TTTGGTGGAA AGACAGGAAT GAAGGGACCA 
CAGGGCCCTG TTGGCATGTA TGGTTTTCCA GGAACCGTGG GTATGAGAGG AAGACCAGGC 
CACACTGGAG ACAGAGGAGA GCCAGGCCCA AGAGGCCCTA CTGGACCAAT TGGAAAGACT 
GGCGTACCAG GTCCCCCTGG TCCAGTAGGA GAAAAAGGAC TGCCAGGACC CCAGGGCCGC 
CAAGGAGATC CTGGGCATCA GGGAACAGAT GGCCCATGTG GTCCTCCAGG ACGTCACGGA 
GTCAGTGGGG TCATTGGAAA ACCAGGACCT CGTGGAAGTA TAGGTCCCCC AGGGGAACCT 
GGGCCAAGTG GGCCCCCAGG AATACCAGGT CCACAGGGGG AAAACGGAGT GCCAGGTATA 
GATGGAGAGA AAGGAGAAAA GGGAATGATG GGTTCTCCAG GTGATGATGG ACCTCTTGGA 
CTGGAAGGAC AACAGGGTCT AACTGGTCCT GCAGGTTTGG ATGGGGCTCC TGGAAGGAAG 
GGTGATAAGG GGGATAGAGG ACCTATCGGA ACGTCTGGTC TTCAAGGACC AAAAGGCGAT 
ATTGGCCCTG TAGGTGATAA AGGTCCACCG GGTCCACCAG GCCTAGTAGG CAATGAGGGT 
GATTCTGGTC CTGCTGGTGT TGCTGGTGAC CCCGGGTCAA AAGGAGAAAA GGGTGATATT 
GGGCCACCAG GATTTACTGG TTTAGAGGGT CCATGGGGAG ACACAGGGGA GCAGGGAGAG 
AAGGGGATCA AGGGAGCCAA GGGACAAATC GGAATGCAGG GTGAGCCAGG CCTAGTTGGT 
GGGGTTGGAC ATCCTGGCCT TAAAGGTGCA GAAGGTGTAC CTGGCCATCC AGGACTGGTT 
GGGCCAGATG GACCTGCAGG AAGCAAGGGA AAGACCGGGC CAAGTGGTTC ACCTGGTGAG 
CCAGGAAATC CTGGACCTGA GGGAATTGCT GGTCCCAAAG GTGTGCGAGG TGACACAGGA 
AAAGGCGGGA CACAGGGTGG TGTGGGTCTA GTTGGTCCCA TAGGTCCTTT GGGCCCCAAA 
GGTCTTTCAG GGCCTGAAGG CGAGAAAGGA GAGCCAGGAG ATAAAGGAGA ACCAGGGTTA 
GAGGGACCAG ATGGCCTTCA GGGTGTACAA GGACCACCAG GACTGCCTGG TTTGGAGGGT 
TCGCAAGGTG AGGCAGGGAT GAGGGGCCCA CCAGGTCTGC CAGGAGCTCA GGGCGGCGTG 
GGTAAGCCAG GACCTGACGG AAGAGAAGGC ATGAAAGGAG AGAAGGGGGA TCAAGGTAAA 
AATGGAGCAC CTGGAAAATT GGGTCACATT GGACGAAGGG GTAAAAGAGG CAAGGCAGGT 
TTGAGGGCGA CCAGAGGACC AAGGGGAGAA AAGGGAGAGA AAGGAGATGT TGGTGAGAAA 
GGACTTCCTG GATGGGGAGG GAGTATGGGA ATCCAAGGGC CAAGAGGAGA GCCTGGAGAT 
GAAGGTGTAA AAGGAGCAGA GGGTGAGAAA GGGGATCAGG GACCATTTGG TTCACCTGGG 
AGAGATGGCA TAAAGGGAAG TCTTGGACCT CTTGGCCCTC CTGGACCCCC AGGACCAAAG 
GGTGATCAGG GAAATATTGG TCCTTCTGGT CCTAGAGGGA CACCTGGAAT ACCTGGACTG 
CCGGGCTTGT TTGGAGACAA GGGACTGAAA GGATTTCAAG GGCCTCCTGG ACCCCCTGGA 
ACAAGGGGTC TGCCAGGTCC ACCAGGGCCA CCAGGACCTC CTGGTATCTC TGTGAACCTG 
ACTCTGATCC AGATCAAGGA CCTGATGTAC AGTTCAGACA AGCCCAATTT CTCCCTGATA 
CAGACACTGC TGGACTCACT GTACCATGAC CTCCAGCTGC TGGTGGACCC TCCGGACGGC 
ACTAAGCAGC ACCCAGCATC AACCTGTCTG GAGCTGTGGC TGTGCCATCC CAACTACACG 
AGTGGTTTCT ACTACATTGA TCCAAACCAA GGCAGTCCTT TAGATGCACT TTTAGCCTAC 
TGTAATTTCT CTGAGTCCGG AGCAGAGACC TGTCTTCACC CGAGAGATGC ACATCTGCCC 
ACAAGGACTT GGCTAAATGA CTCAGGAAAT AACAGCAAGT TCCACTGGTT AAGCTCACTA 
GAGGGGGGTT TTAAATTTGA GTATCCAGCC CTAAGTGTGG TTCAGCTGCG CTTTCTGAGA 
CTCCAGAGCA ACAGAGCGGA GCAGAGAGTG ACGTACTCCT GTTACCCTGG ACACAGACTG 
GGAGAAACAC AGCGACAGGT CCAGTTCCTC ACAGACGCCG CCAAGCAGAG CTATTTAGGA 
GCACTGCAGG ACTGTGTGTC TGCAAAGGAA GTGGATCCTG GGCCGCGTGA GTCTGTGTTT 
CAGTTTGAGG ACATGGACCT GCTTCCTCTA CGTGATATAG GGATCTTTGG AAACAGCAGT 
CAAGAGTTTG GCTTCACCAT TGGCCCTGTA TGCTTCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293536.1
CDS	170..5611
Translation	MLVSVVWTAVLFTLVTASITDTSPTDVPDTESGSGMFLPDAIDLLDHLWQVANHSNASVTHESKRCPVLHVGQYSTLSVPLRQVFGYSFGDEFSLLLHLRSSQSEDRSVLTFLCPDGHILLQIRISAYTFSFISTQQRHYEFPVGVLSDGGWHRVAVSVSSSRLALYVDCSMVESVEWAYKGGLEISTDGLVMLGGIIEGFETPFEGDLMQVAFLIGEPDAAQDHCAFFQPQCGGTGHKPPRSPRIHTEEELLLSSNDLEDLLESAKNSDSIRAKLNGLRRRGIIRGDGTLPTGSKRPAIVGRGDVFEVDEDTDLLDQNILSKNGDKPSKTFPTDTTSSRDGKLESTSKHLDENITIDKHKPGKPLPKKPVDTTIINLDVSEKPTGSTVPSREIHLMTTSSPEDGESNLLKEDFEVTTQAPGRTSWDNVSKGSGRTPSKSPDIHAGSGGGSEEHPDTIVLTGRQGDIVRGSDGKMYRIQRGPMGPMGPPGEPGCPGNAGYPGPKGDKGAFGVRGRPGRPGFLGSPGPPGLPSFYLWRNTPEDWAAFQQTSFFQLLLAGWPRAQGLPGPEGEMGKPGAQGPPGEPGERGPPGRMGDMGDRGPKGVVGRAGVYGRDGENGLDGPQGPSGIPGPKGPLGYKGESGSHGEKGEEGITGSEGPCGDKGKPGEKGSKGAPGAPGAVGPPGPQGIRGMEGLEGHPGPDGEDGMNGSPGLPGAPGAPGWTGLIGAQGANGSRGEPGPSGAVGLPGPQGPQGLEGQIGPPGQRGPPGLSGREGLHGPKGEPGPVGPVGIRGDPGFEGLKGVLGTPGPKGFTGIKGNRGPDGDQGDIGPKGNKGARGAAGVQGIQGEQGPIGFPGFPGIRGPPGPQGNQGEDGEAGPHGKVGKEGSTGSRGPEGLSGKPGPRGLKGRTGQRGHTGMPGPPGLPGPPGPFGAEGKPGTQGLQGLVGNAGNKGSTGLQGIPGERGQDGPPGTMGPPGREGPMGSPGPPGERGFGGKTGMKGPQGPVGMYGFPGTVGMRGRPGHTGDRGEPGPRGPTGPIGKTGVPGPPGPVGEKGLPGPQGRQGDPGHQGTDGPCGPPGRHGVSGVIGKPGPRGSIGPPGEPGPSGPPGIPGPQGENGVPGIDGEKGEKGMMGSPGDDGPLGLEGQQGLTGPAGLDGAPGRKGDKGDRGPIGTSGLQGPKGDIGPVGDKGPPGPPGLVGNEGDSGPAGVAGDPGSKGEKGDIGPPGFTGLEGPWGDTGEQGEKGIKGAKGQIGMQGEPGLVGGVGHPGLKGAEGVPGHPGLVGPDGPAGSKGKTGPSGSPGEPGNPGPEGIAGPKGVRGDTGKGGTQGGVGLVGPIGPLGPKGLSGPEGEKGEPGDKGEPGLEGPDGLQGVQGPPGLPGLEGSQGEAGMRGPPGLPGAQGGVGKPGPDGREGMKGEKGDQGKNGAPGKLGHIGRRGKRGKAGLRATRGPRGEKGEKGDVGEKGLPGWGGSMGIQGPRGEPGDEGVKGAEGEKGDQGPFGSPGRDGIKGSLGPLGPPGPPGPKGDQGNIGPSGPRGTPGIPGLPGLFGDKGLKGFQGPPGPPGTRGLPGPPGPPGPPGISVNLTLIQIKDLMYSSDKPNFSLIQTLLDSLYHDLQLLVDPPDGTKQHPASTCLELWLCHPNYTSGFYYIDPNQGSPLDALLAYCNFSESGAETCLHPRDAHLPTRTWLNDSGNNSKFHWLSSLEGGFKFEYPALSVVQLRFLRLQSNRAEQRVTYSCYPGHRLGETQRQVQFLTDAAKQSYLGALQDCVSAKEVDPGPRESVFQFEDMDLLPLRDIGIFGNSSQEFGFTIGPVCFS

Target ORF information:

RefSeq Version	XM_009295261.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295261.3

ORF Insert Sequence:

ATGCTCGTGT CAGTAGTGTG GACAGCAGTT TTATTTACTC TGGTAACAGC CTCCATCACG 
GACACCAGTC CTACTGACGT ACCAGACACG GAGAGCGGTT CGGGGATGTT TCTACCAGAT 
GCAATCGACC TTCTTGATCA TCTATGGCAG GTTGCTAATC ACAGTAATGC ATCGGTAACG 
CATGAAAGCA AAAGGTGTCC AGTTCTGCAT GTAGGCCAGT ACTCAACACT TTCTGTCCCA 
CTACGGCAGG TGTTCGGATA CAGTTTTGGT GATGAGTTCA GTTTGCTGTT GCATCTGCGC 
AGCTCTCAGT CTGAAGACCG CAGTGTGTTG ACCTTCCTCT GTCCAGATGG ACACATTCTT 
CTGCAGATCC GCATCAGTGC CTACACTTTT AGCTTCATCA GCACTCAACA GCGACACTAC 
GAATTCCCCG TGGGAGTACT GTCTGATGGA GGTTGGCACC GTGTGGCAGT CAGCGTTTCC 
TCGAGTCGGT TGGCATTGTA TGTGGATTGC TCCATGGTGG AGAGTGTGGA ATGGGCCTAT 
AAGGGCGGTC TGGAAATCTC CACCGATGGC CTGGTTATGT TGGGAGGAAT CATTGAGGGG 
TTTGAGACCC CTTTTGAGGG CGATTTGATG CAAGTGGCAT TTCTGATTGG AGAACCAGAT 
GCAGCTCAAG ATCACTGTGC CTTTTTCCAG CCCCAGTGTG GTGGAACTGG ACACAAACCA 
CCTCGCTCTC CTCGCATACA CACTGAAGAG GAACTTTTGC TATCCTCTAA TGACCTGGAG 
GACTTGTTAG AGTCAGCAAA GAACAGTGAC TCTATTCGGG CAAAACTGAA TGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGTAC ACAGGGACTG 
CAAGGCCTTG TGGGTAATGC CGGAAATAAA GGATCAACGG GATTGCAAGG TATTCCCGGA 
GAACGTGGTC AAGACGGACC CCCAGGAACT ATGGGCCCAC CTGGACGAGA GGGGCCTATG 
GGCAGCCCAG GACCACCAGG AGAAAGGGGT TTTGGTGGAA AGACAGGAAT GAAGGGACCA 
CAGGGCCCTG TTGGCATGTA TGGTTTTCCA GGAACCGTGG GTATGAGAGG AAGACCAGGC 
CACACTGGAG ACAGAGGAGA GCCAGGCCCA AGAGGCCCTA CTGGACCAAT TGGAAAGACT 
GGCGTACCAG GTCCCCCTGG TCCAGTAGGA GAAAAAGGAC TGCCAGGACC CCAGGGCCGC 
CAAGGAGATC CTGGGCATCA GGGAACAGAT GGCCCATGTG GTCCTCCAGG ACGTCACGGA 
GTCAGTGGGG TCATTGGAAA ACCAGGACCT CGTGGAAGTA TAGGTCCCCC AGGGGAACCT 
GGGCCAAGTG GGCCCCCAGG AATACCAGGT CCACAGGGGG AAAACGGAGT GCCAGGTATA 
GATGGAGAGA AAGGAGAAAA GGGAATGATG GGTTCTCCAG GTGATGATGG ACCTCTTGGA 
CTGGAAGGAC AACAGGGTCT AACTGGTCCT GCAGGTTTGG ATGGGGCTCC TGGAAGGAAG 
GGTGATAAGG GGGATAGAGG ACCTATCGGA ACGTCTGGTC TTCAAGGACC AAAAGGCGAT 
ATTGGCCCTG TAGGTGATAA AGGTCCACCG GGTCCACCAG GCCTAGTAGG CAATGAGGGT 
GATTCTGGTC CTGCTGGTGT TGCTGGTGAC CCCGGGTCAA AAGGAGAAAA GGGTGATATT 
GGGCCACCAG GATTTACTGG TTTAGAGGGT CCATGGGGAG ACACAGGGGA GCAGGGAGAG 
AAGGGGATCA AGGGAGCCAA GGGACAAATC GGAATGCAGG GTGAGCCAGG CCTAGTTGGT 
GGGGTTGGAC ATCCTGGCCT TAAAGGTGCA GAAGGTGTAC CTGGCCATCC AGGACTGGTT 
GGGCCAGATG GACCTGCAGG AAGCAAGGGA AAGACCGGGC CAAGTGGTTC ACCTGGTGAG 
CCAGGAAATC CTGGACCTGA GGGAATTGCT GGTCCCAAAG GTGTGCGAGG TGACACAGGA 
AAAGGCGGGA CACAGGGTGG TGTGGGTCTA GTTGGTCCCA TAGGTCCTTT GGGCCCCAAA 
GGTCTTTCAG GGCCTGAAGG CGAGAAAGGA GAGCCAGGAG ATAAAGGAGA ACCAGGGTTA 
GAGGGACCAG ATGGCCTTCA GGGTGTACAA GGACCACCAG GACTGCCTGG TTTGGAGGGT 
TCGCAAGGTG AGGCAGGGAT GAGGGGCCCA CCAGGTCTGC CAGGAGCTCA GGGCGGCGTG 
GGTAAGCCAG GACCTGACGG AAGAGAAGGC ATGAAAGGAG AGAAGGGGGA TCAAGGTAAA 
AATGGAGCAC CTGGAAAATT GGGTCACATT GGACGAAGGG GTAAAAGAGG CAAGGCAGGT 
TTGAGGGCGA CCAGAGGACC AAGGGGAGAA AAGGGAGAGA AAGGAGATGT TGGTGAGAAA 
GGACTTCCTG GATGGGGAGG GAGTATGGGA ATCCAAGGGC CAAGAGGAGA GCCTGGAGAT 
GAAGGTGTAA AAGGAGCAGA GGGTGAGAAA GGGGATCAGG GACCATTTGG TTCACCTGGG 
AGAGATGGCA TAAAGGGAAG TCTTGGACCT CTTGGCCCTC CTGGACCCCC AGGACCAAAG 
GGTGATCAGG GAAATATTGG TCCTTCTGGT CCTAGAGGGA CACCTGGAAT ACCTGGACTG 
CCGGGCTTGT TTGGAGACAA GGGACTGAAA GGATTTCAAG GGCCTCCTGG ACCCCCTGGA 
ACAAGGGGTC TGCCAGGTCC ACCAGGGCCA CCAGGACCTC CTGGTATCTC TGTGAACCTG 
ACTCTGATCC AGATCAAGGA CCTGATGTAC AGTTCAGACA AGCCCAATTT CTCCCTGATA 
CAGACACTGC TGGACTCACT GTACCATGAC CTCCAGCTGC TGGTGGACCC TCCGGACGGC 
ACTAAGCAGC ACCCAGCATC AACCTGTCTG GAGCTGTGGC TGTGCCATCC CAACTACACG 
AGTGGTTTCT ACTACATTGA TCCAAACCAA GGCAGTCCTT TAGATGCACT TTTAGCCTAC 
TGTAATTTCT CTGAGTCCGG AGCAGAGACC TGTCTTCACC CGAGAGATGC ACATCTGCCC 
ACAAGGACTT GGCTAAATGA CTCAGGAAAT AACAGCAAGT TCCACTGGTT AAGCTCACTA 
GAGGGGGGTT TTAAATTTGA GTATCCAGCC CTAAGTGTGG TTCAGCTGCG CTTTCTGAGA 
CTCCAGAGCA ACAGAGCGGA GCAGAGAGTG ACGTACTCCT GTTACCCTGG ACACAGACTG 
GGAGAAACAC AGCGACAGGT CCAGTTCCTC ACAGACGCCG CCAAGCAGAG CTATTTAGGA 
GCACTGCAGG ACTGTGTGTC TGCAAAGGAA GTGGATCCTG GGCCGCGTGA GTCTGTGTTT 
CAGTTTGAGG ACATGGACCT GCTTCCTCTA CGTGATATAG GGATCTTTGG AAACAGCAGT 
CAAGAGTTTG GCTTCACCAT TGGCCCTGTA TGCTTCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-196i2.1 ( XM_009295261.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293536.1 Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X1, mRNA.

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CloneID	ODa44242
Clone ID Related Accession (Same CDS sequence)	XM_009295261.3 , XM_009295261.2
Accession Version	XM_009295261.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(I) chain-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGTGT CAGTAGTGTG GACAGCAGTT TTATTTACTC TGGTAACAGC CTCCATCACG 
GACACCAGTC CTACTGACGT ACCAGACACG GAGAGCGGTT CGGGGATGTT TCTACCAGAT 
GCAATCGACC TTCTTGATCA TCTATGGCAG GTTGCTAATC ACAGTAATGC ATCGGTAACG 
CATGAAAGCA AAAGGTGTCC AGTTCTGCAT GTAGGCCAGT ACTCAACACT TTCTGTCCCA 
CTACGGCAGG TGTTCGGATA CAGTTTTGGT GATGAGTTCA GTTTGCTGTT GCATCTGCGC 
AGCTCTCAGT CTGAAGACCG CAGTGTGTTG ACCTTCCTCT GTCCAGATGG ACACATTCTT 
CTGCAGATCC GCATCAGTGC CTACACTTTT AGCTTCATCA GCACTCAACA GCGACACTAC 
GAATTCCCCG TGGGAGTACT GTCTGATGGA GGTTGGCACC GTGTGGCAGT CAGCGTTTCC 
TCGAGTCGGT TGGCATTGTA TGTGGATTGC TCCATGGTGG AGAGTGTGGA ATGGGCCTAT 
AAGGGCGGTC TGGAAATCTC CACCGATGGC CTGGTTATGT TGGGAGGAAT CATTGAGGGG 
TTTGAGACCC CTTTTGAGGG CGATTTGATG CAAGTGGCAT TTCTGATTGG AGAACCAGAT 
GCAGCTCAAG ATCACTGTGC CTTTTTCCAG CCCCAGTGTG GTGGAACTGG ACACAAACCA 
CCTCGCTCTC CTCGCATACA CACTGAAGAG GAACTTTTGC TATCCTCTAA TGACCTGGAG 
GACTTGTTAG AGTCAGCAAA GAACAGTGAC TCTATTCGGG CAAAACTGAA TGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGTAC ACAGGGACTG 
CAAGGCCTTG TGGGTAATGC CGGAAATAAA GGATCAACGG GATTGCAAGG TATTCCCGGA 
GAACGTGGTC AAGACGGACC CCCAGGAACT ATGGGCCCAC CTGGACGAGA GGGGCCTATG 
GGCAGCCCAG GACCACCAGG AGAAAGGGGT TTTGGTGGAA AGACAGGAAT GAAGGGACCA 
CAGGGCCCTG TTGGCATGTA TGGTTTTCCA GGAACCGTGG GTATGAGAGG AAGACCAGGC 
CACACTGGAG ACAGAGGAGA GCCAGGCCCA AGAGGCCCTA CTGGACCAAT TGGAAAGACT 
GGCGTACCAG GTCCCCCTGG TCCAGTAGGA GAAAAAGGAC TGCCAGGACC CCAGGGCCGC 
CAAGGAGATC CTGGGCATCA GGGAACAGAT GGCCCATGTG GTCCTCCAGG ACGTCACGGA 
GTCAGTGGGG TCATTGGAAA ACCAGGACCT CGTGGAAGTA TAGGTCCCCC AGGGGAACCT 
GGGCCAAGTG GGCCCCCAGG AATACCAGGT CCACAGGGGG AAAACGGAGT GCCAGGTATA 
GATGGAGAGA AAGGAGAAAA GGGAATGATG GGTTCTCCAG GTGATGATGG ACCTCTTGGA 
CTGGAAGGAC AACAGGGTCT AACTGGTCCT GCAGGTTTGG ATGGGGCTCC TGGAAGGAAG 
GGTGATAAGG GGGATAGAGG ACCTATCGGA ACGTCTGGTC TTCAAGGACC AAAAGGCGAT 
ATTGGCCCTG TAGGTGATAA AGGTCCACCG GGTCCACCAG GCCTAGTAGG CAATGAGGGT 
GATTCTGGTC CTGCTGGTGT TGCTGGTGAC CCCGGGTCAA AAGGAGAAAA GGGTGATATT 
GGGCCACCAG GATTTACTGG TTTAGAGGGT CCATGGGGAG ACACAGGGGA GCAGGGAGAG 
AAGGGGATCA AGGGAGCCAA GGGACAAATC GGAATGCAGG GTGAGCCAGG CCTAGTTGGT 
GGGGTTGGAC ATCCTGGCCT TAAAGGTGCA GAAGGTGTAC CTGGCCATCC AGGACTGGTT 
GGGCCAGATG GACCTGCAGG AAGCAAGGGA AAGACCGGGC CAAGTGGTTC ACCTGGTGAG 
CCAGGAAATC CTGGACCTGA GGGAATTGCT GGTCCCAAAG GTGTGCGAGG TGACACAGGA 
AAAGGCGGGA CACAGGGTGG TGTGGGTCTA GTTGGTCCCA TAGGTCCTTT GGGCCCCAAA 
GGTCTTTCAG GGCCTGAAGG CGAGAAAGGA GAGCCAGGAG ATAAAGGAGA ACCAGGGTTA 
GAGGGACCAG ATGGCCTTCA GGGTGTACAA GGACCACCAG GACTGCCTGG TTTGGAGGGT 
TCGCAAGGTG AGGCAGGGAT GAGGGGCCCA CCAGGTCTGC CAGGAGCTCA GGGCGGCGTG 
GGTAAGCCAG GACCTGACGG AAGAGAAGGC ATGAAAGGAG AGAAGGGGGA TCAAGGTAAA 
AATGGAGCAC CTGGAAAATT GGGTCACATT GGACGAAGGG GTAAAAGAGG CAAGGCAGGT 
TTGAGGGCGA CCAGAGGACC AAGGGGAGAA AAGGGAGAGA AAGGAGATGT TGGTGAGAAA 
GGACTTCCTG GATGGGGAGG GAGTATGGGA ATCCAAGGGC CAAGAGGAGA GCCTGGAGAT 
GAAGGTGTAA AAGGAGCAGA GGGTGAGAAA GGGGATCAGG GACCATTTGG TTCACCTGGG 
AGAGATGGCA TAAAGGGAAG TCTTGGACCT CTTGGCCCTC CTGGACCCCC AGGACCAAAG 
GGTGATCAGG GAAATATTGG TCCTTCTGGT CCTAGAGGGA CACCTGGAAT ACCTGGACTG 
CCGGGCTTGT TTGGAGACAA GGGACTGAAA GGATTTCAAG GGCCTCCTGG ACCCCCTGGA 
ACAAGGGGTC TGCCAGGTCC ACCAGGGCCA CCAGGACCTC CTGGTATCTC TGTGAACCTG 
ACTCTGATCC AGATCAAGGA CCTGATGTAC AGTTCAGACA AGCCCAATTT CTCCCTGATA 
CAGACACTGC TGGACTCACT GTACCATGAC CTCCAGCTGC TGGTGGACCC TCCGGACGGC 
ACTAAGCAGC ACCCAGCATC AACCTGTCTG GAGCTGTGGC TGTGCCATCC CAACTACACG 
AGTGGTTTCT ACTACATTGA TCCAAACCAA GGCAGTCCTT TAGATGCACT TTTAGCCTAC 
TGTAATTTCT CTGAGTCCGG AGCAGAGACC TGTCTTCACC CGAGAGATGC ACATCTGCCC 
ACAAGGACTT GGCTAAATGA CTCAGGAAAT AACAGCAAGT TCCACTGGTT AAGCTCACTA 
GAGGGGGGTT TTAAATTTGA GTATCCAGCC CTAAGTGTGG TTCAGCTGCG CTTTCTGAGA 
CTCCAGAGCA ACAGAGCGGA GCAGAGAGTG ACGTACTCCT GTTACCCTGG ACACAGACTG 
GGAGAAACAC AGCGACAGGT CCAGTTCCTC ACAGACGCCG CCAAGCAGAG CTATTTAGGA 
GCACTGCAGG ACTGTGTGTC TGCAAAGGAA GTGGATCCTG GGCCGCGTGA GTCTGTGTTT 
CAGTTTGAGG ACATGGACCT GCTTCCTCTA CGTGATATAG GGATCTTTGG AAACAGCAGT 
CAAGAGTTTG GCTTCACCAT TGGCCCTGTA TGCTTCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293536.1
CDS	167..5608
Translation	MLVSVVWTAVLFTLVTASITDTSPTDVPDTESGSGMFLPDAIDLLDHLWQVANHSNASVTHESKRCPVLHVGQYSTLSVPLRQVFGYSFGDEFSLLLHLRSSQSEDRSVLTFLCPDGHILLQIRISAYTFSFISTQQRHYEFPVGVLSDGGWHRVAVSVSSSRLALYVDCSMVESVEWAYKGGLEISTDGLVMLGGIIEGFETPFEGDLMQVAFLIGEPDAAQDHCAFFQPQCGGTGHKPPRSPRIHTEEELLLSSNDLEDLLESAKNSDSIRAKLNGLRRRGIIRGDGTLPTGSKRPAIVGRGDVFEVDEDTDLLDQNILSKNGDKPSKTFPTDTTSSRDGKLESTSKHLDENITIDKHKPGKPLPKKPVDTTIINLDVSEKPTGSTVPSREIHLMTTSSPEDGESNLLKEDFEVTTQAPGRTSWDNVSKGSGRTPSKSPDIHAGSGGGSEEHPDTIVLTGRQGDIVRGSDGKMYRIQRGPMGPMGPPGEPGCPGNAGYPGPKGDKGAFGVRGRPGRPGFLGSPGPPGLPSFYLWRNTPEDWAAFQQTSFFQLLLAGWPRAQGLPGPEGEMGKPGAQGPPGEPGERGPPGRMGDMGDRGPKGVVGRAGVYGRDGENGLDGPQGPSGIPGPKGPLGYKGESGSHGEKGEEGITGSEGPCGDKGKPGEKGSKGAPGAPGAVGPPGPQGIRGMEGLEGHPGPDGEDGMNGSPGLPGAPGAPGWTGLIGAQGANGSRGEPGPSGAVGLPGPQGPQGLEGQIGPPGQRGPPGLSGREGLHGPKGEPGPVGPVGIRGDPGFEGLKGVLGTPGPKGFTGIKGNRGPDGDQGDIGPKGNKGARGAAGVQGIQGEQGPIGFPGFPGIRGPPGPQGNQGEDGEAGPHGKVGKEGSTGSRGPEGLSGKPGPRGLKGRTGQRGHTGMPGPPGLPGPPGPFGAEGKPGTQGLQGLVGNAGNKGSTGLQGIPGERGQDGPPGTMGPPGREGPMGSPGPPGERGFGGKTGMKGPQGPVGMYGFPGTVGMRGRPGHTGDRGEPGPRGPTGPIGKTGVPGPPGPVGEKGLPGPQGRQGDPGHQGTDGPCGPPGRHGVSGVIGKPGPRGSIGPPGEPGPSGPPGIPGPQGENGVPGIDGEKGEKGMMGSPGDDGPLGLEGQQGLTGPAGLDGAPGRKGDKGDRGPIGTSGLQGPKGDIGPVGDKGPPGPPGLVGNEGDSGPAGVAGDPGSKGEKGDIGPPGFTGLEGPWGDTGEQGEKGIKGAKGQIGMQGEPGLVGGVGHPGLKGAEGVPGHPGLVGPDGPAGSKGKTGPSGSPGEPGNPGPEGIAGPKGVRGDTGKGGTQGGVGLVGPIGPLGPKGLSGPEGEKGEPGDKGEPGLEGPDGLQGVQGPPGLPGLEGSQGEAGMRGPPGLPGAQGGVGKPGPDGREGMKGEKGDQGKNGAPGKLGHIGRRGKRGKAGLRATRGPRGEKGEKGDVGEKGLPGWGGSMGIQGPRGEPGDEGVKGAEGEKGDQGPFGSPGRDGIKGSLGPLGPPGPPGPKGDQGNIGPSGPRGTPGIPGLPGLFGDKGLKGFQGPPGPPGTRGLPGPPGPPGPPGISVNLTLIQIKDLMYSSDKPNFSLIQTLLDSLYHDLQLLVDPPDGTKQHPASTCLELWLCHPNYTSGFYYIDPNQGSPLDALLAYCNFSESGAETCLHPRDAHLPTRTWLNDSGNNSKFHWLSSLEGGFKFEYPALSVVQLRFLRLQSNRAEQRVTYSCYPGHRLGETQRQVQFLTDAAKQSYLGALQDCVSAKEVDPGPRESVFQFEDMDLLPLRDIGIFGNSSQEFGFTIGPVCFS

Target ORF information:

RefSeq Version	XM_009295261.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295261.2

ORF Insert Sequence:

ATGCTCGTGT CAGTAGTGTG GACAGCAGTT TTATTTACTC TGGTAACAGC CTCCATCACG 
GACACCAGTC CTACTGACGT ACCAGACACG GAGAGCGGTT CGGGGATGTT TCTACCAGAT 
GCAATCGACC TTCTTGATCA TCTATGGCAG GTTGCTAATC ACAGTAATGC ATCGGTAACG 
CATGAAAGCA AAAGGTGTCC AGTTCTGCAT GTAGGCCAGT ACTCAACACT TTCTGTCCCA 
CTACGGCAGG TGTTCGGATA CAGTTTTGGT GATGAGTTCA GTTTGCTGTT GCATCTGCGC 
AGCTCTCAGT CTGAAGACCG CAGTGTGTTG ACCTTCCTCT GTCCAGATGG ACACATTCTT 
CTGCAGATCC GCATCAGTGC CTACACTTTT AGCTTCATCA GCACTCAACA GCGACACTAC 
GAATTCCCCG TGGGAGTACT GTCTGATGGA GGTTGGCACC GTGTGGCAGT CAGCGTTTCC 
TCGAGTCGGT TGGCATTGTA TGTGGATTGC TCCATGGTGG AGAGTGTGGA ATGGGCCTAT 
AAGGGCGGTC TGGAAATCTC CACCGATGGC CTGGTTATGT TGGGAGGAAT CATTGAGGGG 
TTTGAGACCC CTTTTGAGGG CGATTTGATG CAAGTGGCAT TTCTGATTGG AGAACCAGAT 
GCAGCTCAAG ATCACTGTGC CTTTTTCCAG CCCCAGTGTG GTGGAACTGG ACACAAACCA 
CCTCGCTCTC CTCGCATACA CACTGAAGAG GAACTTTTGC TATCCTCTAA TGACCTGGAG 
GACTTGTTAG AGTCAGCAAA GAACAGTGAC TCTATTCGGG CAAAACTGAA TGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGTAC ACAGGGACTG 
CAAGGCCTTG TGGGTAATGC CGGAAATAAA GGATCAACGG GATTGCAAGG TATTCCCGGA 
GAACGTGGTC AAGACGGACC CCCAGGAACT ATGGGCCCAC CTGGACGAGA GGGGCCTATG 
GGCAGCCCAG GACCACCAGG AGAAAGGGGT TTTGGTGGAA AGACAGGAAT GAAGGGACCA 
CAGGGCCCTG TTGGCATGTA TGGTTTTCCA GGAACCGTGG GTATGAGAGG AAGACCAGGC 
CACACTGGAG ACAGAGGAGA GCCAGGCCCA AGAGGCCCTA CTGGACCAAT TGGAAAGACT 
GGCGTACCAG GTCCCCCTGG TCCAGTAGGA GAAAAAGGAC TGCCAGGACC CCAGGGCCGC 
CAAGGAGATC CTGGGCATCA GGGAACAGAT GGCCCATGTG GTCCTCCAGG ACGTCACGGA 
GTCAGTGGGG TCATTGGAAA ACCAGGACCT CGTGGAAGTA TAGGTCCCCC AGGGGAACCT 
GGGCCAAGTG GGCCCCCAGG AATACCAGGT CCACAGGGGG AAAACGGAGT GCCAGGTATA 
GATGGAGAGA AAGGAGAAAA GGGAATGATG GGTTCTCCAG GTGATGATGG ACCTCTTGGA 
CTGGAAGGAC AACAGGGTCT AACTGGTCCT GCAGGTTTGG ATGGGGCTCC TGGAAGGAAG 
GGTGATAAGG GGGATAGAGG ACCTATCGGA ACGTCTGGTC TTCAAGGACC AAAAGGCGAT 
ATTGGCCCTG TAGGTGATAA AGGTCCACCG GGTCCACCAG GCCTAGTAGG CAATGAGGGT 
GATTCTGGTC CTGCTGGTGT TGCTGGTGAC CCCGGGTCAA AAGGAGAAAA GGGTGATATT 
GGGCCACCAG GATTTACTGG TTTAGAGGGT CCATGGGGAG ACACAGGGGA GCAGGGAGAG 
AAGGGGATCA AGGGAGCCAA GGGACAAATC GGAATGCAGG GTGAGCCAGG CCTAGTTGGT 
GGGGTTGGAC ATCCTGGCCT TAAAGGTGCA GAAGGTGTAC CTGGCCATCC AGGACTGGTT 
GGGCCAGATG GACCTGCAGG AAGCAAGGGA AAGACCGGGC CAAGTGGTTC ACCTGGTGAG 
CCAGGAAATC CTGGACCTGA GGGAATTGCT GGTCCCAAAG GTGTGCGAGG TGACACAGGA 
AAAGGCGGGA CACAGGGTGG TGTGGGTCTA GTTGGTCCCA TAGGTCCTTT GGGCCCCAAA 
GGTCTTTCAG GGCCTGAAGG CGAGAAAGGA GAGCCAGGAG ATAAAGGAGA ACCAGGGTTA 
GAGGGACCAG ATGGCCTTCA GGGTGTACAA GGACCACCAG GACTGCCTGG TTTGGAGGGT 
TCGCAAGGTG AGGCAGGGAT GAGGGGCCCA CCAGGTCTGC CAGGAGCTCA GGGCGGCGTG 
GGTAAGCCAG GACCTGACGG AAGAGAAGGC ATGAAAGGAG AGAAGGGGGA TCAAGGTAAA 
AATGGAGCAC CTGGAAAATT GGGTCACATT GGACGAAGGG GTAAAAGAGG CAAGGCAGGT 
TTGAGGGCGA CCAGAGGACC AAGGGGAGAA AAGGGAGAGA AAGGAGATGT TGGTGAGAAA 
GGACTTCCTG GATGGGGAGG GAGTATGGGA ATCCAAGGGC CAAGAGGAGA GCCTGGAGAT 
GAAGGTGTAA AAGGAGCAGA GGGTGAGAAA GGGGATCAGG GACCATTTGG TTCACCTGGG 
AGAGATGGCA TAAAGGGAAG TCTTGGACCT CTTGGCCCTC CTGGACCCCC AGGACCAAAG 
GGTGATCAGG GAAATATTGG TCCTTCTGGT CCTAGAGGGA CACCTGGAAT ACCTGGACTG 
CCGGGCTTGT TTGGAGACAA GGGACTGAAA GGATTTCAAG GGCCTCCTGG ACCCCCTGGA 
ACAAGGGGTC TGCCAGGTCC ACCAGGGCCA CCAGGACCTC CTGGTATCTC TGTGAACCTG 
ACTCTGATCC AGATCAAGGA CCTGATGTAC AGTTCAGACA AGCCCAATTT CTCCCTGATA 
CAGACACTGC TGGACTCACT GTACCATGAC CTCCAGCTGC TGGTGGACCC TCCGGACGGC 
ACTAAGCAGC ACCCAGCATC AACCTGTCTG GAGCTGTGGC TGTGCCATCC CAACTACACG 
AGTGGTTTCT ACTACATTGA TCCAAACCAA GGCAGTCCTT TAGATGCACT TTTAGCCTAC 
TGTAATTTCT CTGAGTCCGG AGCAGAGACC TGTCTTCACC CGAGAGATGC ACATCTGCCC 
ACAAGGACTT GGCTAAATGA CTCAGGAAAT AACAGCAAGT TCCACTGGTT AAGCTCACTA 
GAGGGGGGTT TTAAATTTGA GTATCCAGCC CTAAGTGTGG TTCAGCTGCG CTTTCTGAGA 
CTCCAGAGCA ACAGAGCGGA GCAGAGAGTG ACGTACTCCT GTTACCCTGG ACACAGACTG 
GGAGAAACAC AGCGACAGGT CCAGTTCCTC ACAGACGCCG CCAAGCAGAG CTATTTAGGA 
GCACTGCAGG ACTGTGTGTC TGCAAAGGAA GTGGATCCTG GGCCGCGTGA GTCTGTGTTT 
CAGTTTGAGG ACATGGACCT GCTTCCTCTA CGTGATATAG GGATCTTTGG AAACAGCAGT 
CAAGAGTTTG GCTTCACCAT TGGCCCTGTA TGCTTCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-196i2.1 ( XM_009295262.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293537.1 Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X2, mRNA.

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CloneID	ODa44243
Clone ID Related Accession (Same CDS sequence)	XM_009295262.2 , XM_009295262.3
Accession Version	XM_009295262.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(I) chain-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGAAT GTGTGTGTGC CATCATGAGG ATCTGGATGT GTTTGCTAGC ATGCTTTCTG 
TTGGATTTAA TGAGATTTGG AGCGCAAGGT CAGAGGAATC GCATTCCAAA GGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGTAC ACAGGGACTG 
CAAGGCCTTG TGGGTAATGC CGGAAATAAA GGATCAACGG GATTGCAAGG TATTCCCGGA 
GAACGTGGTC AAGACGGACC CCCAGGAACT ATGGGCCCAC CTGGACGAGA GGGGCCTATG 
GGCAGCCCAG GACCACCAGG AGAAAGGGGT TTTGGTGGAA AGACAGGAAT GAAGGGACCA 
CAGGGCCCTG TTGGCATGTA TGGTTTTCCA GGAACCGTGG GTATGAGAGG AAGACCAGGC 
CACACTGGAG ACAGAGGAGA GCCAGGCCCA AGAGGCCCTA CTGGACCAAT TGGAAAGACT 
GGCGTACCAG GTCCCCCTGG TCCAGTAGGA GAAAAAGGAC TGCCAGGACC CCAGGGCCGC 
CAAGGAGATC CTGGGCATCA GGGAACAGAT GGCCCATGTG GTCCTCCAGG ACGTCACGGA 
GTCAGTGGGG TCATTGGAAA ACCAGGACCT CGTGGAAGTA TAGGTCCCCC AGGGGAACCT 
GGGCCAAGTG GGCCCCCAGG AATACCAGGT CCACAGGGGG AAAACGGAGT GCCAGGTATA 
GATGGAGAGA AAGGAGAAAA GGGAATGATG GGTTCTCCAG GTGATGATGG ACCTCTTGGA 
CTGGAAGGAC AACAGGGTCT AACTGGTCCT GCAGGTTTGG ATGGGGCTCC TGGAAGGAAG 
GGTGATAAGG GGGATAGAGG ACCTATCGGA ACGTCTGGTC TTCAAGGACC AAAAGGCGAT 
ATTGGCCCTG TAGGTGATAA AGGTCCACCG GGTCCACCAG GCCTAGTAGG CAATGAGGGT 
GATTCTGGTC CTGCTGGTGT TGCTGGTGAC CCCGGGTCAA AAGGAGAAAA GGGTGATATT 
GGGCCACCAG GATTTACTGG TTTAGAGGGT CCATGGGGAG ACACAGGGGA GCAGGGAGAG 
AAGGGGATCA AGGGAGCCAA GGGACAAATC GGAATGCAGG GTGAGCCAGG CCTAGTTGGT 
GGGGTTGGAC ATCCTGGCCT TAAAGGTGCA GAAGGTGTAC CTGGCCATCC AGGACTGGTT 
GGGCCAGATG GACCTGCAGG AAGCAAGGGA AAGACCGGGC CAAGTGGTTC ACCTGGTGAG 
CCAGGAAATC CTGGACCTGA GGGAATTGCT GGTCCCAAAG GTGTGCGAGG TGACACAGGA 
AAAGGCGGGA CACAGGGTGG TGTGGGTCTA GTTGGTCCCA TAGGTCCTTT GGGCCCCAAA 
GGTCTTTCAG GGCCTGAAGG CGAGAAAGGA GAGCCAGGAG ATAAAGGAGA ACCAGGGTTA 
GAGGGACCAG ATGGCCTTCA GGGTGTACAA GGACCACCAG GACTGCCTGG TTTGGAGGGT 
TCGCAAGGTG AGGCAGGGAT GAGGGGCCCA CCAGGTCTGC CAGGAGCTCA GGGCGGCGTG 
GGTAAGCCAG GACCTGACGG AAGAGAAGGC ATGAAAGGAG AGAAGGGGGA TCAAGGTAAA 
AATGGAGCAC CTGGAAAATT GGGTCACATT GGACGAAGGG GTAAAAGAGG CAAGGCAGGT 
TTGAGGGCGA CCAGAGGACC AAGGGGAGAA AAGGGAGAGA AAGGAGATGT TGGTGAGAAA 
GGACTTCCTG GATGGGGAGG GAGTATGGGA ATCCAAGGGC CAAGAGGAGA GCCTGGAGAT 
GAAGGTGTAA AAGGAGCAGA GGGTGAGAAA GGGGATCAGG GACCATTTGG TTCACCTGGG 
AGAGATGGCA TAAAGGGAAG TCTTGGACCT CTTGGCCCTC CTGGACCCCC AGGACCAAAG 
GGTGATCAGG GAAATATTGG TCCTTCTGGT CCTAGAGGGA CACCTGGAAT ACCTGGACTG 
CCGGGCTTGT TTGGAGACAA GGGACTGAAA GGATTTCAAG GGCCTCCTGG ACCCCCTGGA 
ACAAGGGGTC TGCCAGGTCC ACCAGGGCCA CCAGGACCTC CTGGTATCTC TGTGAACCTG 
ACTCTGATCC AGATCAAGGA CCTGATGTAC AGTTCAGACA AGCCCAATTT CTCCCTGATA 
CAGACACTGC TGGACTCACT GTACCATGAC CTCCAGCTGC TGGTGGACCC TCCGGACGGC 
ACTAAGCAGC ACCCAGCATC AACCTGTCTG GAGCTGTGGC TGTGCCATCC CAACTACACG 
AGTGGTTTCT ACTACATTGA TCCAAACCAA GGCAGTCCTT TAGATGCACT TTTAGCCTAC 
TGTAATTTCT CTGAGTCCGG AGCAGAGACC TGTCTTCACC CGAGAGATGC ACATCTGCCC 
ACAAGGACTT GGCTAAATGA CTCAGGAAAT AACAGCAAGT TCCACTGGTT AAGCTCACTA 
GAGGGGGGTT TTAAATTTGA GTATCCAGCC CTAAGTGTGG TTCAGCTGCG CTTTCTGAGA 
CTCCAGAGCA ACAGAGCGGA GCAGAGAGTG ACGTACTCCT GTTACCCTGG ACACAGACTG 
GGAGAAACAC AGCGACAGGT CCAGTTCCTC ACAGACGCCG CCAAGCAGAG CTATTTAGGA 
GCACTGCAGG ACTGTGTGTC TGCAAAGGAA GTGGATCCTG GGCCGCGTGA GTCTGTGTTT 
CAGTTTGAGG ACATGGACCT GCTTCCTCTA CGTGATATAG GGATCTTTGG AAACAGCAGT 
CAAGAGTTTG GCTTCACCAT TGGCCCTGTA TGCTTCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293537.1
CDS	19..4740
Translation	MCECVCAIMRIWMCLLACFLLDLMRFGAQGQRNRIPKGLRRRGIIRGDGTLPTGSKRPAIVGRGDVFEVDEDTDLLDQNILSKNGDKPSKTFPTDTTSSRDGKLESTSKHLDENITIDKHKPGKPLPKKPVDTTIINLDVSEKPTGSTVPSREIHLMTTSSPEDGESNLLKEDFEVTTQAPGRTSWDNVSKGSGRTPSKSPDIHAGSGGGSEEHPDTIVLTGRQGDIVRGSDGKMYRIQRGPMGPMGPPGEPGCPGNAGYPGPKGDKGAFGVRGRPGRPGFLGSPGPPGLPSFYLWRNTPEDWAAFQQTSFFQLLLAGWPRAQGLPGPEGEMGKPGAQGPPGEPGERGPPGRMGDMGDRGPKGVVGRAGVYGRDGENGLDGPQGPSGIPGPKGPLGYKGESGSHGEKGEEGITGSEGPCGDKGKPGEKGSKGAPGAPGAVGPPGPQGIRGMEGLEGHPGPDGEDGMNGSPGLPGAPGAPGWTGLIGAQGANGSRGEPGPSGAVGLPGPQGPQGLEGQIGPPGQRGPPGLSGREGLHGPKGEPGPVGPVGIRGDPGFEGLKGVLGTPGPKGFTGIKGNRGPDGDQGDIGPKGNKGARGAAGVQGIQGEQGPIGFPGFPGIRGPPGPQGNQGEDGEAGPHGKVGKEGSTGSRGPEGLSGKPGPRGLKGRTGQRGHTGMPGPPGLPGPPGPFGAEGKPGTQGLQGLVGNAGNKGSTGLQGIPGERGQDGPPGTMGPPGREGPMGSPGPPGERGFGGKTGMKGPQGPVGMYGFPGTVGMRGRPGHTGDRGEPGPRGPTGPIGKTGVPGPPGPVGEKGLPGPQGRQGDPGHQGTDGPCGPPGRHGVSGVIGKPGPRGSIGPPGEPGPSGPPGIPGPQGENGVPGIDGEKGEKGMMGSPGDDGPLGLEGQQGLTGPAGLDGAPGRKGDKGDRGPIGTSGLQGPKGDIGPVGDKGPPGPPGLVGNEGDSGPAGVAGDPGSKGEKGDIGPPGFTGLEGPWGDTGEQGEKGIKGAKGQIGMQGEPGLVGGVGHPGLKGAEGVPGHPGLVGPDGPAGSKGKTGPSGSPGEPGNPGPEGIAGPKGVRGDTGKGGTQGGVGLVGPIGPLGPKGLSGPEGEKGEPGDKGEPGLEGPDGLQGVQGPPGLPGLEGSQGEAGMRGPPGLPGAQGGVGKPGPDGREGMKGEKGDQGKNGAPGKLGHIGRRGKRGKAGLRATRGPRGEKGEKGDVGEKGLPGWGGSMGIQGPRGEPGDEGVKGAEGEKGDQGPFGSPGRDGIKGSLGPLGPPGPPGPKGDQGNIGPSGPRGTPGIPGLPGLFGDKGLKGFQGPPGPPGTRGLPGPPGPPGPPGISVNLTLIQIKDLMYSSDKPNFSLIQTLLDSLYHDLQLLVDPPDGTKQHPASTCLELWLCHPNYTSGFYYIDPNQGSPLDALLAYCNFSESGAETCLHPRDAHLPTRTWLNDSGNNSKFHWLSSLEGGFKFEYPALSVVQLRFLRLQSNRAEQRVTYSCYPGHRLGETQRQVQFLTDAAKQSYLGALQDCVSAKEVDPGPRESVFQFEDMDLLPLRDIGIFGNSSQEFGFTIGPVCFS

Target ORF information:

RefSeq Version	XM_009295262.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295262.2

ORF Insert Sequence:

ATGTGTGAAT GTGTGTGTGC CATCATGAGG ATCTGGATGT GTTTGCTAGC ATGCTTTCTG 
TTGGATTTAA TGAGATTTGG AGCGCAAGGT CAGAGGAATC GCATTCCAAA GGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGTAC ACAGGGACTG 
CAAGGCCTTG TGGGTAATGC CGGAAATAAA GGATCAACGG GATTGCAAGG TATTCCCGGA 
GAACGTGGTC AAGACGGACC CCCAGGAACT ATGGGCCCAC CTGGACGAGA GGGGCCTATG 
GGCAGCCCAG GACCACCAGG AGAAAGGGGT TTTGGTGGAA AGACAGGAAT GAAGGGACCA 
CAGGGCCCTG TTGGCATGTA TGGTTTTCCA GGAACCGTGG GTATGAGAGG AAGACCAGGC 
CACACTGGAG ACAGAGGAGA GCCAGGCCCA AGAGGCCCTA CTGGACCAAT TGGAAAGACT 
GGCGTACCAG GTCCCCCTGG TCCAGTAGGA GAAAAAGGAC TGCCAGGACC CCAGGGCCGC 
CAAGGAGATC CTGGGCATCA GGGAACAGAT GGCCCATGTG GTCCTCCAGG ACGTCACGGA 
GTCAGTGGGG TCATTGGAAA ACCAGGACCT CGTGGAAGTA TAGGTCCCCC AGGGGAACCT 
GGGCCAAGTG GGCCCCCAGG AATACCAGGT CCACAGGGGG AAAACGGAGT GCCAGGTATA 
GATGGAGAGA AAGGAGAAAA GGGAATGATG GGTTCTCCAG GTGATGATGG ACCTCTTGGA 
CTGGAAGGAC AACAGGGTCT AACTGGTCCT GCAGGTTTGG ATGGGGCTCC TGGAAGGAAG 
GGTGATAAGG GGGATAGAGG ACCTATCGGA ACGTCTGGTC TTCAAGGACC AAAAGGCGAT 
ATTGGCCCTG TAGGTGATAA AGGTCCACCG GGTCCACCAG GCCTAGTAGG CAATGAGGGT 
GATTCTGGTC CTGCTGGTGT TGCTGGTGAC CCCGGGTCAA AAGGAGAAAA GGGTGATATT 
GGGCCACCAG GATTTACTGG TTTAGAGGGT CCATGGGGAG ACACAGGGGA GCAGGGAGAG 
AAGGGGATCA AGGGAGCCAA GGGACAAATC GGAATGCAGG GTGAGCCAGG CCTAGTTGGT 
GGGGTTGGAC ATCCTGGCCT TAAAGGTGCA GAAGGTGTAC CTGGCCATCC AGGACTGGTT 
GGGCCAGATG GACCTGCAGG AAGCAAGGGA AAGACCGGGC CAAGTGGTTC ACCTGGTGAG 
CCAGGAAATC CTGGACCTGA GGGAATTGCT GGTCCCAAAG GTGTGCGAGG TGACACAGGA 
AAAGGCGGGA CACAGGGTGG TGTGGGTCTA GTTGGTCCCA TAGGTCCTTT GGGCCCCAAA 
GGTCTTTCAG GGCCTGAAGG CGAGAAAGGA GAGCCAGGAG ATAAAGGAGA ACCAGGGTTA 
GAGGGACCAG ATGGCCTTCA GGGTGTACAA GGACCACCAG GACTGCCTGG TTTGGAGGGT 
TCGCAAGGTG AGGCAGGGAT GAGGGGCCCA CCAGGTCTGC CAGGAGCTCA GGGCGGCGTG 
GGTAAGCCAG GACCTGACGG AAGAGAAGGC ATGAAAGGAG AGAAGGGGGA TCAAGGTAAA 
AATGGAGCAC CTGGAAAATT GGGTCACATT GGACGAAGGG GTAAAAGAGG CAAGGCAGGT 
TTGAGGGCGA CCAGAGGACC AAGGGGAGAA AAGGGAGAGA AAGGAGATGT TGGTGAGAAA 
GGACTTCCTG GATGGGGAGG GAGTATGGGA ATCCAAGGGC CAAGAGGAGA GCCTGGAGAT 
GAAGGTGTAA AAGGAGCAGA GGGTGAGAAA GGGGATCAGG GACCATTTGG TTCACCTGGG 
AGAGATGGCA TAAAGGGAAG TCTTGGACCT CTTGGCCCTC CTGGACCCCC AGGACCAAAG 
GGTGATCAGG GAAATATTGG TCCTTCTGGT CCTAGAGGGA CACCTGGAAT ACCTGGACTG 
CCGGGCTTGT TTGGAGACAA GGGACTGAAA GGATTTCAAG GGCCTCCTGG ACCCCCTGGA 
ACAAGGGGTC TGCCAGGTCC ACCAGGGCCA CCAGGACCTC CTGGTATCTC TGTGAACCTG 
ACTCTGATCC AGATCAAGGA CCTGATGTAC AGTTCAGACA AGCCCAATTT CTCCCTGATA 
CAGACACTGC TGGACTCACT GTACCATGAC CTCCAGCTGC TGGTGGACCC TCCGGACGGC 
ACTAAGCAGC ACCCAGCATC AACCTGTCTG GAGCTGTGGC TGTGCCATCC CAACTACACG 
AGTGGTTTCT ACTACATTGA TCCAAACCAA GGCAGTCCTT TAGATGCACT TTTAGCCTAC 
TGTAATTTCT CTGAGTCCGG AGCAGAGACC TGTCTTCACC CGAGAGATGC ACATCTGCCC 
ACAAGGACTT GGCTAAATGA CTCAGGAAAT AACAGCAAGT TCCACTGGTT AAGCTCACTA 
GAGGGGGGTT TTAAATTTGA GTATCCAGCC CTAAGTGTGG TTCAGCTGCG CTTTCTGAGA 
CTCCAGAGCA ACAGAGCGGA GCAGAGAGTG ACGTACTCCT GTTACCCTGG ACACAGACTG 
GGAGAAACAC AGCGACAGGT CCAGTTCCTC ACAGACGCCG CCAAGCAGAG CTATTTAGGA 
GCACTGCAGG ACTGTGTGTC TGCAAAGGAA GTGGATCCTG GGCCGCGTGA GTCTGTGTTT 
CAGTTTGAGG ACATGGACCT GCTTCCTCTA CGTGATATAG GGATCTTTGG AAACAGCAGT 
CAAGAGTTTG GCTTCACCAT TGGCCCTGTA TGCTTCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-196i2.1 ( XM_009295262.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293537.1 Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X2, mRNA.

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CloneID	ODa44243
Clone ID Related Accession (Same CDS sequence)	XM_009295262.2 , XM_009295262.3
Accession Version	XM_009295262.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(I) chain-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295262.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGAAT GTGTGTGTGC CATCATGAGG ATCTGGATGT GTTTGCTAGC ATGCTTTCTG 
TTGGATTTAA TGAGATTTGG AGCGCAAGGT CAGAGGAATC GCATTCCAAA GGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGTAC ACAGGGACTG 
CAAGGCCTTG TGGGTAATGC CGGAAATAAA GGATCAACGG GATTGCAAGG TATTCCCGGA 
GAACGTGGTC AAGACGGACC CCCAGGAACT ATGGGCCCAC CTGGACGAGA GGGGCCTATG 
GGCAGCCCAG GACCACCAGG AGAAAGGGGT TTTGGTGGAA AGACAGGAAT GAAGGGACCA 
CAGGGCCCTG TTGGCATGTA TGGTTTTCCA GGAACCGTGG GTATGAGAGG AAGACCAGGC 
CACACTGGAG ACAGAGGAGA GCCAGGCCCA AGAGGCCCTA CTGGACCAAT TGGAAAGACT 
GGCGTACCAG GTCCCCCTGG TCCAGTAGGA GAAAAAGGAC TGCCAGGACC CCAGGGCCGC 
CAAGGAGATC CTGGGCATCA GGGAACAGAT GGCCCATGTG GTCCTCCAGG ACGTCACGGA 
GTCAGTGGGG TCATTGGAAA ACCAGGACCT CGTGGAAGTA TAGGTCCCCC AGGGGAACCT 
GGGCCAAGTG GGCCCCCAGG AATACCAGGT CCACAGGGGG AAAACGGAGT GCCAGGTATA 
GATGGAGAGA AAGGAGAAAA GGGAATGATG GGTTCTCCAG GTGATGATGG ACCTCTTGGA 
CTGGAAGGAC AACAGGGTCT AACTGGTCCT GCAGGTTTGG ATGGGGCTCC TGGAAGGAAG 
GGTGATAAGG GGGATAGAGG ACCTATCGGA ACGTCTGGTC TTCAAGGACC AAAAGGCGAT 
ATTGGCCCTG TAGGTGATAA AGGTCCACCG GGTCCACCAG GCCTAGTAGG CAATGAGGGT 
GATTCTGGTC CTGCTGGTGT TGCTGGTGAC CCCGGGTCAA AAGGAGAAAA GGGTGATATT 
GGGCCACCAG GATTTACTGG TTTAGAGGGT CCATGGGGAG ACACAGGGGA GCAGGGAGAG 
AAGGGGATCA AGGGAGCCAA GGGACAAATC GGAATGCAGG GTGAGCCAGG CCTAGTTGGT 
GGGGTTGGAC ATCCTGGCCT TAAAGGTGCA GAAGGTGTAC CTGGCCATCC AGGACTGGTT 
GGGCCAGATG GACCTGCAGG AAGCAAGGGA AAGACCGGGC CAAGTGGTTC ACCTGGTGAG 
CCAGGAAATC CTGGACCTGA GGGAATTGCT GGTCCCAAAG GTGTGCGAGG TGACACAGGA 
AAAGGCGGGA CACAGGGTGG TGTGGGTCTA GTTGGTCCCA TAGGTCCTTT GGGCCCCAAA 
GGTCTTTCAG GGCCTGAAGG CGAGAAAGGA GAGCCAGGAG ATAAAGGAGA ACCAGGGTTA 
GAGGGACCAG ATGGCCTTCA GGGTGTACAA GGACCACCAG GACTGCCTGG TTTGGAGGGT 
TCGCAAGGTG AGGCAGGGAT GAGGGGCCCA CCAGGTCTGC CAGGAGCTCA GGGCGGCGTG 
GGTAAGCCAG GACCTGACGG AAGAGAAGGC ATGAAAGGAG AGAAGGGGGA TCAAGGTAAA 
AATGGAGCAC CTGGAAAATT GGGTCACATT GGACGAAGGG GTAAAAGAGG CAAGGCAGGT 
TTGAGGGCGA CCAGAGGACC AAGGGGAGAA AAGGGAGAGA AAGGAGATGT TGGTGAGAAA 
GGACTTCCTG GATGGGGAGG GAGTATGGGA ATCCAAGGGC CAAGAGGAGA GCCTGGAGAT 
GAAGGTGTAA AAGGAGCAGA GGGTGAGAAA GGGGATCAGG GACCATTTGG TTCACCTGGG 
AGAGATGGCA TAAAGGGAAG TCTTGGACCT CTTGGCCCTC CTGGACCCCC AGGACCAAAG 
GGTGATCAGG GAAATATTGG TCCTTCTGGT CCTAGAGGGA CACCTGGAAT ACCTGGACTG 
CCGGGCTTGT TTGGAGACAA GGGACTGAAA GGATTTCAAG GGCCTCCTGG ACCCCCTGGA 
ACAAGGGGTC TGCCAGGTCC ACCAGGGCCA CCAGGACCTC CTGGTATCTC TGTGAACCTG 
ACTCTGATCC AGATCAAGGA CCTGATGTAC AGTTCAGACA AGCCCAATTT CTCCCTGATA 
CAGACACTGC TGGACTCACT GTACCATGAC CTCCAGCTGC TGGTGGACCC TCCGGACGGC 
ACTAAGCAGC ACCCAGCATC AACCTGTCTG GAGCTGTGGC TGTGCCATCC CAACTACACG 
AGTGGTTTCT ACTACATTGA TCCAAACCAA GGCAGTCCTT TAGATGCACT TTTAGCCTAC 
TGTAATTTCT CTGAGTCCGG AGCAGAGACC TGTCTTCACC CGAGAGATGC ACATCTGCCC 
ACAAGGACTT GGCTAAATGA CTCAGGAAAT AACAGCAAGT TCCACTGGTT AAGCTCACTA 
GAGGGGGGTT TTAAATTTGA GTATCCAGCC CTAAGTGTGG TTCAGCTGCG CTTTCTGAGA 
CTCCAGAGCA ACAGAGCGGA GCAGAGAGTG ACGTACTCCT GTTACCCTGG ACACAGACTG 
GGAGAAACAC AGCGACAGGT CCAGTTCCTC ACAGACGCCG CCAAGCAGAG CTATTTAGGA 
GCACTGCAGG ACTGTGTGTC TGCAAAGGAA GTGGATCCTG GGCCGCGTGA GTCTGTGTTT 
CAGTTTGAGG ACATGGACCT GCTTCCTCTA CGTGATATAG GGATCTTTGG AAACAGCAGT 
CAAGAGTTTG GCTTCACCAT TGGCCCTGTA TGCTTCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293537.1
CDS	49..4770
Translation	MCECVCAIMRIWMCLLACFLLDLMRFGAQGQRNRIPKGLRRRGIIRGDGTLPTGSKRPAIVGRGDVFEVDEDTDLLDQNILSKNGDKPSKTFPTDTTSSRDGKLESTSKHLDENITIDKHKPGKPLPKKPVDTTIINLDVSEKPTGSTVPSREIHLMTTSSPEDGESNLLKEDFEVTTQAPGRTSWDNVSKGSGRTPSKSPDIHAGSGGGSEEHPDTIVLTGRQGDIVRGSDGKMYRIQRGPMGPMGPPGEPGCPGNAGYPGPKGDKGAFGVRGRPGRPGFLGSPGPPGLPSFYLWRNTPEDWAAFQQTSFFQLLLAGWPRAQGLPGPEGEMGKPGAQGPPGEPGERGPPGRMGDMGDRGPKGVVGRAGVYGRDGENGLDGPQGPSGIPGPKGPLGYKGESGSHGEKGEEGITGSEGPCGDKGKPGEKGSKGAPGAPGAVGPPGPQGIRGMEGLEGHPGPDGEDGMNGSPGLPGAPGAPGWTGLIGAQGANGSRGEPGPSGAVGLPGPQGPQGLEGQIGPPGQRGPPGLSGREGLHGPKGEPGPVGPVGIRGDPGFEGLKGVLGTPGPKGFTGIKGNRGPDGDQGDIGPKGNKGARGAAGVQGIQGEQGPIGFPGFPGIRGPPGPQGNQGEDGEAGPHGKVGKEGSTGSRGPEGLSGKPGPRGLKGRTGQRGHTGMPGPPGLPGPPGPFGAEGKPGTQGLQGLVGNAGNKGSTGLQGIPGERGQDGPPGTMGPPGREGPMGSPGPPGERGFGGKTGMKGPQGPVGMYGFPGTVGMRGRPGHTGDRGEPGPRGPTGPIGKTGVPGPPGPVGEKGLPGPQGRQGDPGHQGTDGPCGPPGRHGVSGVIGKPGPRGSIGPPGEPGPSGPPGIPGPQGENGVPGIDGEKGEKGMMGSPGDDGPLGLEGQQGLTGPAGLDGAPGRKGDKGDRGPIGTSGLQGPKGDIGPVGDKGPPGPPGLVGNEGDSGPAGVAGDPGSKGEKGDIGPPGFTGLEGPWGDTGEQGEKGIKGAKGQIGMQGEPGLVGGVGHPGLKGAEGVPGHPGLVGPDGPAGSKGKTGPSGSPGEPGNPGPEGIAGPKGVRGDTGKGGTQGGVGLVGPIGPLGPKGLSGPEGEKGEPGDKGEPGLEGPDGLQGVQGPPGLPGLEGSQGEAGMRGPPGLPGAQGGVGKPGPDGREGMKGEKGDQGKNGAPGKLGHIGRRGKRGKAGLRATRGPRGEKGEKGDVGEKGLPGWGGSMGIQGPRGEPGDEGVKGAEGEKGDQGPFGSPGRDGIKGSLGPLGPPGPPGPKGDQGNIGPSGPRGTPGIPGLPGLFGDKGLKGFQGPPGPPGTRGLPGPPGPPGPPGISVNLTLIQIKDLMYSSDKPNFSLIQTLLDSLYHDLQLLVDPPDGTKQHPASTCLELWLCHPNYTSGFYYIDPNQGSPLDALLAYCNFSESGAETCLHPRDAHLPTRTWLNDSGNNSKFHWLSSLEGGFKFEYPALSVVQLRFLRLQSNRAEQRVTYSCYPGHRLGETQRQVQFLTDAAKQSYLGALQDCVSAKEVDPGPRESVFQFEDMDLLPLRDIGIFGNSSQEFGFTIGPVCFS

Target ORF information:

RefSeq Version	XM_009295262.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-196i2.1 (si:ch211-196i2.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295262.3

ORF Insert Sequence:

ATGTGTGAAT GTGTGTGTGC CATCATGAGG ATCTGGATGT GTTTGCTAGC ATGCTTTCTG 
TTGGATTTAA TGAGATTTGG AGCGCAAGGT CAGAGGAATC GCATTCCAAA GGGTCTTCGT 
AGACGTGGGA TTATTCGTGG GGATGGGACT TTGCCCACTG GATCCAAGCG TCCTGCAATT 
GTGGGCCGTG GAGATGTGTT TGAGGTGGAT GAGGATACAG ACCTTCTGGA CCAAAACATC 
TTGTCTAAAA ATGGAGATAA ACCTTCCAAA ACCTTCCCAA CTGACACTAC AAGCAGCCGT 
GATGGTAAGC TAGAATCCAC ATCTAAACAT CTTGATGAAA ACATCACCAT TGACAAGCAC 
AAACCTGGCA AACCTTTACC AAAGAAGCCA GTGGACACTA CAATTATCAA TCTGGATGTA 
TCTGAGAAAC CTACTGGAAG TACTGTTCCT AGTAGAGAAA TCCACTTGAT GACCACATCC 
AGTCCTGAGG ATGGAGAATC CAACTTGTTA AAAGAGGACT TCGAGGTCAC CACACAGGCT 
CCTGGTAGGA CTTCTTGGGA CAATGTGTCA AAAGGCAGTG GAAGGACACC ATCGAAATCT 
CCAGATATTC ATGCAGGATC TGGAGGGGGA TCTGAGGAGC ATCCGGATAC CATTGTGCTT 
ACAGGCAGAC AAGGAGACAT TGTGCGTGGG TCTGATGGGA AAATGTATCG CATCCAAAGA 
GGGCCTATGG GGCCAATGGG GCCCCCAGGT GAGCCTGGGT GTCCTGGGAA TGCTGGCTAT 
CCTGGACCCA AAGGAGATAA GGGTGCCTTT GGTGTTAGGG GTCGTCCAGG TCGCCCTGGA 
TTCCTGGGAT CCCCAGGACC ACCCGGCCTG CCCTCATTCT ACCTATGGAG AAACACACCT 
GAAGACTGGG CTGCATTTCA GCAAACATCA TTCTTCCAGC TTCTTCTTGC AGGCTGGCCT 
AGAGCACAAG GTCTGCCTGG TCCGGAAGGA GAGATGGGAA AACCTGGGGC ACAGGGGCCA 
CCTGGGGAAC CAGGTGAACG AGGACCCCCA GGACGAATGG GAGACATGGG TGACCGTGGT 
CCTAAAGGTG TGGTTGGAAG AGCGGGAGTT TATGGAAGAG ATGGAGAGAA CGGCCTGGAC 
GGCCCTCAGG GGCCTTCAGG GATTCCAGGC CCAAAGGGTC CTCTGGGTTA TAAAGGTGAA 
TCAGGCTCAC ATGGAGAGAA AGGAGAGGAG GGCATTACTG GATCAGAGGG TCCATGTGGA 
GATAAGGGGA AACCCGGTGA GAAAGGTTCA AAAGGAGCAC CTGGCGCTCC TGGGGCTGTC 
GGCCCTCCTG GTCCTCAAGG CATAAGAGGG ATGGAGGGCC TTGAAGGGCA TCCAGGACCA 
GATGGGGAGG ATGGGATGAA TGGATCACCA GGTCTTCCAG GTGCACCAGG AGCTCCTGGT 
TGGACAGGTT TGATTGGTGC CCAGGGGGCT AATGGGTCTC GTGGGGAACC TGGGCCTTCT 
GGAGCTGTTG GACTTCCAGG GCCACAGGGG CCCCAGGGAT TAGAAGGACA GATCGGACCT 
CCAGGACAAA GAGGTCCACC GGGGTTGTCA GGCCGAGAGG GTTTGCATGG TCCCAAAGGA 
GAGCCTGGTC CTGTAGGACC AGTTGGTATC AGAGGGGATC CAGGATTTGA AGGATTAAAG 
GGTGTTTTAG GAACGCCCGG TCCAAAAGGT TTCACTGGTA TCAAAGGTAA CAGAGGACCT 
GATGGAGACC AAGGGGACAT TGGACCTAAA GGAAACAAAG GTGCTAGAGG AGCAGCAGGT 
GTACAGGGCA TCCAAGGTGA ACAGGGACCA ATAGGCTTTC CTGGCTTTCC AGGCATCAGA 
GGCCCACCAG GACCACAGGG AAACCAGGGA GAGGATGGAG AGGCTGGTCC ACATGGTAAA 
GTGGGCAAAG AGGGGTCAAC GGGGAGCAGA GGTCCTGAAG GCCTCTCTGG AAAGCCTGGT 
CCTAGAGGCC TAAAGGGGCG AACAGGGCAG AGGGGTCACA CTGGCATGCC TGGACCTCCT 
GGTTTACCTG GCCCACCTGG ACCATTCGGA GCTGAAGGAA AGCCTGGTAC ACAGGGACTG 
CAAGGCCTTG TGGGTAATGC CGGAAATAAA GGATCAACGG GATTGCAAGG TATTCCCGGA 
GAACGTGGTC AAGACGGACC CCCAGGAACT ATGGGCCCAC CTGGACGAGA GGGGCCTATG 
GGCAGCCCAG GACCACCAGG AGAAAGGGGT TTTGGTGGAA AGACAGGAAT GAAGGGACCA 
CAGGGCCCTG TTGGCATGTA TGGTTTTCCA GGAACCGTGG GTATGAGAGG AAGACCAGGC 
CACACTGGAG ACAGAGGAGA GCCAGGCCCA AGAGGCCCTA CTGGACCAAT TGGAAAGACT 
GGCGTACCAG GTCCCCCTGG TCCAGTAGGA GAAAAAGGAC TGCCAGGACC CCAGGGCCGC 
CAAGGAGATC CTGGGCATCA GGGAACAGAT GGCCCATGTG GTCCTCCAGG ACGTCACGGA 
GTCAGTGGGG TCATTGGAAA ACCAGGACCT CGTGGAAGTA TAGGTCCCCC AGGGGAACCT 
GGGCCAAGTG GGCCCCCAGG AATACCAGGT CCACAGGGGG AAAACGGAGT GCCAGGTATA 
GATGGAGAGA AAGGAGAAAA GGGAATGATG GGTTCTCCAG GTGATGATGG ACCTCTTGGA 
CTGGAAGGAC AACAGGGTCT AACTGGTCCT GCAGGTTTGG ATGGGGCTCC TGGAAGGAAG 
GGTGATAAGG GGGATAGAGG ACCTATCGGA ACGTCTGGTC TTCAAGGACC AAAAGGCGAT 
ATTGGCCCTG TAGGTGATAA AGGTCCACCG GGTCCACCAG GCCTAGTAGG CAATGAGGGT 
GATTCTGGTC CTGCTGGTGT TGCTGGTGAC CCCGGGTCAA AAGGAGAAAA GGGTGATATT 
GGGCCACCAG GATTTACTGG TTTAGAGGGT CCATGGGGAG ACACAGGGGA GCAGGGAGAG 
AAGGGGATCA AGGGAGCCAA GGGACAAATC GGAATGCAGG GTGAGCCAGG CCTAGTTGGT 
GGGGTTGGAC ATCCTGGCCT TAAAGGTGCA GAAGGTGTAC CTGGCCATCC AGGACTGGTT 
GGGCCAGATG GACCTGCAGG AAGCAAGGGA AAGACCGGGC CAAGTGGTTC ACCTGGTGAG 
CCAGGAAATC CTGGACCTGA GGGAATTGCT GGTCCCAAAG GTGTGCGAGG TGACACAGGA 
AAAGGCGGGA CACAGGGTGG TGTGGGTCTA GTTGGTCCCA TAGGTCCTTT GGGCCCCAAA 
GGTCTTTCAG GGCCTGAAGG CGAGAAAGGA GAGCCAGGAG ATAAAGGAGA ACCAGGGTTA 
GAGGGACCAG ATGGCCTTCA GGGTGTACAA GGACCACCAG GACTGCCTGG TTTGGAGGGT 
TCGCAAGGTG AGGCAGGGAT GAGGGGCCCA CCAGGTCTGC CAGGAGCTCA GGGCGGCGTG 
GGTAAGCCAG GACCTGACGG AAGAGAAGGC ATGAAAGGAG AGAAGGGGGA TCAAGGTAAA 
AATGGAGCAC CTGGAAAATT GGGTCACATT GGACGAAGGG GTAAAAGAGG CAAGGCAGGT 
TTGAGGGCGA CCAGAGGACC AAGGGGAGAA AAGGGAGAGA AAGGAGATGT TGGTGAGAAA 
GGACTTCCTG GATGGGGAGG GAGTATGGGA ATCCAAGGGC CAAGAGGAGA GCCTGGAGAT 
GAAGGTGTAA AAGGAGCAGA GGGTGAGAAA GGGGATCAGG GACCATTTGG TTCACCTGGG 
AGAGATGGCA TAAAGGGAAG TCTTGGACCT CTTGGCCCTC CTGGACCCCC AGGACCAAAG 
GGTGATCAGG GAAATATTGG TCCTTCTGGT CCTAGAGGGA CACCTGGAAT ACCTGGACTG 
CCGGGCTTGT TTGGAGACAA GGGACTGAAA GGATTTCAAG GGCCTCCTGG ACCCCCTGGA 
ACAAGGGGTC TGCCAGGTCC ACCAGGGCCA CCAGGACCTC CTGGTATCTC TGTGAACCTG 
ACTCTGATCC AGATCAAGGA CCTGATGTAC AGTTCAGACA AGCCCAATTT CTCCCTGATA 
CAGACACTGC TGGACTCACT GTACCATGAC CTCCAGCTGC TGGTGGACCC TCCGGACGGC 
ACTAAGCAGC ACCCAGCATC AACCTGTCTG GAGCTGTGGC TGTGCCATCC CAACTACACG 
AGTGGTTTCT ACTACATTGA TCCAAACCAA GGCAGTCCTT TAGATGCACT TTTAGCCTAC 
TGTAATTTCT CTGAGTCCGG AGCAGAGACC TGTCTTCACC CGAGAGATGC ACATCTGCCC 
ACAAGGACTT GGCTAAATGA CTCAGGAAAT AACAGCAAGT TCCACTGGTT AAGCTCACTA 
GAGGGGGGTT TTAAATTTGA GTATCCAGCC CTAAGTGTGG TTCAGCTGCG CTTTCTGAGA 
CTCCAGAGCA ACAGAGCGGA GCAGAGAGTG ACGTACTCCT GTTACCCTGG ACACAGACTG 
GGAGAAACAC AGCGACAGGT CCAGTTCCTC ACAGACGCCG CCAAGCAGAG CTATTTAGGA 
GCACTGCAGG ACTGTGTGTC TGCAAAGGAA GTGGATCCTG GGCCGCGTGA GTCTGTGTTT 
CAGTTTGAGG ACATGGACCT GCTTCCTCTA CGTGATATAG GGATCTTTGG AAACAGCAGT 
CAAGAGTTTG GCTTCACCAT TGGCCCTGTA TGCTTCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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si:ch211-196i2.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

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