efcab8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	efcab8
Entrez Gene ID	100494912
Full Name	EF-hand calcium binding domain 8
General protein information	Preferred Names EF-hand calcium binding domain 8 Names WD repeat-containing protein 49-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012954179.2	XP_012809633.1	WD repeat-containing protein 49-like isoform X1
XM_031894727.1	XP_031750587.1	WD repeat-containing protein on Y chromosome isoform X1
XM_012954182.3	XP_012809636.2	WD repeat-containing protein on Y chromosome isoform X3
XM_012954181.2	XP_012809635.1	WD repeat-containing protein 49-like isoform X2
XM_012954182.2	XP_012809636.1	WD repeat-containing protein 49-like isoform X3
XM_031894726.1	XP_031750586.1	WD repeat-containing protein on Y chromosome isoform X1
XM_031894728.1	XP_031750588.1	WD repeat-containing protein on Y chromosome isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100494912	XP_002937050.2
Danio rerio (zebrafish)	si:dkey-202c14.3	XP_002663929.3

Gene Ontology

The following efcab8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the efcab8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa36143	XM_012954179.2 Latest version!	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa36144	XM_012954181.2 Latest version!	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa36145	XM_012954182.2 Latest version!	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa50838	XM_031894726.1 Latest version!	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa50838	XM_031894727.1 Latest version!	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa50839	XM_031894728.1 Latest version!	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa50840	XM_012954182.3 Latest version!	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

efcab8 ( XM_012954179.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809633.1 Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa36143
Clone ID Related Accession (Same CDS sequence)	XM_012954179.2
Accession Version	XM_012954179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	WD repeat-containing protein 49-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTTC CGACTCTTCT TCAGAAACGT GCCAATGCCC TACAGAGACA ACTCCTGTCT 
GTCACTCTAC TTCAAGAGCT AGAACACTTC CTAAAGAAGC AGGGGCTTCA AAAATGTTCT 
GGGCAAAAGG TGAAGTTCTG CAATGAGGAA AGCATCTTAA GCTTCGATCG CAATGAAAAC 
CTGCGCTTGG AGGAGAAGTT CCGTTTGGAT GACCTGCGCC TCCTGCAGGA AGCCTTCGCT 
AAGCACAATT CCGACGGCAA AGAGACCAAT TGCAAAGAGG AGTCCGACGA TCTCCGAGCG 
ACGAGGCGCA GAGCGAAACA GGAGGCGCGG CCTGCGGGAA ACATGACCCT CGATGAATTC 
CGCCGGGTTC TCTGCGACCT CTTCGGGTCG GAATGTTTCG ACGACGACAT GGAACTTCTG 
TTTAACGAGG TGGACACCTC CTGTGACGGC TACGTAGACT GGGGGGAGTT CTGCACTTAC 
CTGCTGCTGC AATACAGGAA GCGAGACTAC AGGATGATTG GCAGGGTGAC CTTCCTGGGA 
CAGCCAGTCA TACGGCTTTG CACTCGCAAC AAGCAAGAGC CAACCAGCAG GATCCTCGCC 
ATCAGCTCCC CCCCTCCCAT GTGGCTCCTC AGTGTGAGCA AAACCGGCGT CCTCACTACG 
TGGGATTTGG CCCTGCACCC CCAGAAATCC TACGAGGTTG CTGCTGAATA CCCAGACTCC 
CAAGCCGGGA AGAGGAGGTT CAAGTGCTGG GTAACGGACG CTGTGCATCT GCCCAACGCT 
AATAAAATCG CCATCGCCAC CACCAGCCGT GACATCCACT TCTTTGACGT CTCCACCATG 
AACTTGTTTG AGGAGTTTCA CCTGTTTGCT CTGAATCACG TTCCCACAAC CTTCTCATAT 
TGGTACAACA AACAGTCCCC AGAGAGTCGG TCCCTGCTGT TGTGGGGAGG AGATAATGGG 
GCGGTGAATG TTCTTTGGCT GCTCCAACCC CAGACGGGGG TATTTGAGAA ACCATTTAGC 
CAGCATCCGG GCCCTACTAA AATCTACATG CAGGAGCTGA AAGATCACAG CAAACTGCTG 
AGCTACCAGG TTATCCCCGA CGTTCACCCC GAGGCCATCA CCAAACTCAT ATACTGCCCG 
GAGAAGCAAC TGATCATTAC ATCATCGGGA AGCGCCCAAT CCTCGCTGCT CATTATGGAC 
ATTAACCAGA AGGGAAAGGT TTATACGTGG AAGATTAACA AGGGGGTGCA GTGTTTCGAC 
TACTGCCGGG CCTTGAATCT GCTGGTAACT GGGGGGTTCG ACCACAAAGT GCGACTATGG 
AATCAATACG TCACGTGTCG CCCCATTGCC GTCCTACCGG AACATTCCAT GGCCATCCTG 
GATGTGATGA TCTATCAGCC CCTGGGGCAA ATATTCAGCT TCTCCAAAGA CTCTGTGCTA 
AAGGTTTGGG ACATCCCCTC ACAGACGTGC CTGCACACCC TGGTGCTGAA GTTCCCCTGT 
ATCCAGCCCG GCAGGATCCA AGAGCAGGGC AGCTTCCCCT TCCTGCTGGT ACCCACCAAC 
CCCAGCCGCT TACTGGTCTG CTACTCCGAC TATATTGGCA TTCTGAGACT GGCACGGGCT 
GAGCCCACGG AGGGCACTTT CTTCACACAC AGGGCACCTG TTACCTGCCT CCTCTACAGC 
AGCTTCTGCC ATCTGATAGT TACGGGCAGC GAAGATTCCA CGGTTGCCGT GTGGGATGTG 
GAGACCGGCG CCAAATCTCT TCTACTCGGC AACGCGCACG GCCAGGAGGA AGTCACTTGC 
ATGGCGCTCG ACAAAGCGCA GAGGAGGCTC ATCACTGGGG CGAGGAACGG CACCATAAAG 
ATCTGGAACA TTCAGAACGG GCACAACCTG CACCAACTGG AGCCGGTGGA GGATGCGGAG 
ATCACTTGCA TCGTGGCCTC GCAGGAGAAT GTGTTCCTCA CTGTCGGCTG GAACCGGAAA 
ATTGTCACTT ACGACATCCC CAAAACCAAT GAGGTGTATG TGCCGGCCAA TCACTCGTGG 
AAAGGAGGGC AACTTCACCA GGAGGATATC CTGACTGCTG ATTTCTGCCC CTCCCTGGGG 
CTTCTTGCTA CAGCCAGTTT TGATGGAGAG ATCATTCTGT GGAACGCGGA GACTCAGAGA 
CTGTATCTGT ATCTGCGGCA GTGTCCCCAA GCAAGGTCTC CGGCACCGGT GGATAAACTC 
TTCTTCCTCC AGAAACGTTC GGCAAAGAGA GAAGCCGCCA TTCTGGTCAG TTCGGAGGCG 
GGGACCCTGC GTTGGTGGCG AGTGACCAAT GGTGGGCAGG AATGGGGGAG TTTCTATGCC 
CAGGATGAGG CAGACTGCTC TGTATTGGGG TTAGCAAGTG ACAAGGCAAA CAGGGTTCTG 
GTATCGGGAG ACACCCTGGG ATTGGTCCAG GTGTGGGACA TTTCCGCCTA TGGGCTACAG 
GCCGCCGCCC AGACCTACAC CTGTAGGCCA CCCCTACTTT ACTCTTGGGG GGCCCATGAC 
AGCGCCGTAG TCAGTGTGGA ACATTTCTTG TACGACTCGG AACCATTCAT TGCCAGCGGC 
TCCTCCGACA AAACGGCCAA ACTGTGGACA GTGGACGGGA AGCAAGTGGG AACGTTCGGC 
CAATCCAAAT GCTGGAAACT AAGTGACCCC ACCTCTTACC TGGACAGCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809633.1
CDS	189..2882
Translation	MPLPTLLQKRANALQRQLLSVTLLQELEHFLKKQGLQKCSGQKVKFCNEESILSFDRNENLRLEEKFRLDDLRLLQEAFAKHNSDGKETNCKEESDDLRATRRRAKQEARPAGNMTLDEFRRVLCDLFGSECFDDDMELLFNEVDTSCDGYVDWGEFCTYLLLQYRKRDYRMIGRVTFLGQPVIRLCTRNKQEPTSRILAISSPPPMWLLSVSKTGVLTTWDLALHPQKSYEVAAEYPDSQAGKRRFKCWVTDAVHLPNANKIAIATTSRDIHFFDVSTMNLFEEFHLFALNHVPTTFSYWYNKQSPESRSLLLWGGDNGAVNVLWLLQPQTGVFEKPFSQHPGPTKIYMQELKDHSKLLSYQVIPDVHPEAITKLIYCPEKQLIITSSGSAQSSLLIMDINQKGKVYTWKINKGVQCFDYCRALNLLVTGGFDHKVRLWNQYVTCRPIAVLPEHSMAILDVMIYQPLGQIFSFSKDSVLKVWDIPSQTCLHTLVLKFPCIQPGRIQEQGSFPFLLVPTNPSRLLVCYSDYIGILRLARAEPTEGTFFTHRAPVTCLLYSSFCHLIVTGSEDSTVAVWDVETGAKSLLLGNAHGQEEVTCMALDKAQRRLITGARNGTIKIWNIQNGHNLHQLEPVEDAEITCIVASQENVFLTVGWNRKIVTYDIPKTNEVYVPANHSWKGGQLHQEDILTADFCPSLGLLATASFDGEIILWNAETQRLYLYLRQCPQARSPAPVDKLFFLQKRSAKREAAILVSSEAGTLRWWRVTNGGQEWGSFYAQDEADCSVLGLASDKANRVLVSGDTLGLVQVWDISAYGLQAAAQTYTCRPPLLYSWGAHDSAVVSVEHFLYDSEPFIASGSSDKTAKLWTVDGKQVGTFGQSKCWKLSDPTSYLDSR

Target ORF information:

RefSeq Version	XM_012954179.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954179.2

ORF Insert Sequence:

ATGCCCCTTC CGACTCTTCT TCAGAAACGT GCCAATGCCC TACAGAGACA ACTCCTGTCT 
GTCACTCTAC TTCAAGAGCT AGAACACTTC CTAAAGAAGC AGGGGCTTCA AAAATGTTCT 
GGGCAAAAGG TGAAGTTCTG CAATGAGGAA AGCATCTTAA GCTTCGATCG CAATGAAAAC 
CTGCGCTTGG AGGAGAAGTT CCGTTTGGAT GACCTGCGCC TCCTGCAGGA AGCCTTCGCT 
AAGCACAATT CCGACGGCAA AGAGACCAAT TGCAAAGAGG AGTCCGACGA TCTCCGAGCG 
ACGAGGCGCA GAGCGAAACA GGAGGCGCGG CCTGCGGGAA ACATGACCCT CGATGAATTC 
CGCCGGGTTC TCTGCGACCT CTTCGGGTCG GAATGTTTCG ACGACGACAT GGAACTTCTG 
TTTAACGAGG TGGACACCTC CTGTGACGGC TACGTAGACT GGGGGGAGTT CTGCACTTAC 
CTGCTGCTGC AATACAGGAA GCGAGACTAC AGGATGATTG GCAGGGTGAC CTTCCTGGGA 
CAGCCAGTCA TACGGCTTTG CACTCGCAAC AAGCAAGAGC CAACCAGCAG GATCCTCGCC 
ATCAGCTCCC CCCCTCCCAT GTGGCTCCTC AGTGTGAGCA AAACCGGCGT CCTCACTACG 
TGGGATTTGG CCCTGCACCC CCAGAAATCC TACGAGGTTG CTGCTGAATA CCCAGACTCC 
CAAGCCGGGA AGAGGAGGTT CAAGTGCTGG GTAACGGACG CTGTGCATCT GCCCAACGCT 
AATAAAATCG CCATCGCCAC CACCAGCCGT GACATCCACT TCTTTGACGT CTCCACCATG 
AACTTGTTTG AGGAGTTTCA CCTGTTTGCT CTGAATCACG TTCCCACAAC CTTCTCATAT 
TGGTACAACA AACAGTCCCC AGAGAGTCGG TCCCTGCTGT TGTGGGGAGG AGATAATGGG 
GCGGTGAATG TTCTTTGGCT GCTCCAACCC CAGACGGGGG TATTTGAGAA ACCATTTAGC 
CAGCATCCGG GCCCTACTAA AATCTACATG CAGGAGCTGA AAGATCACAG CAAACTGCTG 
AGCTACCAGG TTATCCCCGA CGTTCACCCC GAGGCCATCA CCAAACTCAT ATACTGCCCG 
GAGAAGCAAC TGATCATTAC ATCATCGGGA AGCGCCCAAT CCTCGCTGCT CATTATGGAC 
ATTAACCAGA AGGGAAAGGT TTATACGTGG AAGATTAACA AGGGGGTGCA GTGTTTCGAC 
TACTGCCGGG CCTTGAATCT GCTGGTAACT GGGGGGTTCG ACCACAAAGT GCGACTATGG 
AATCAATACG TCACGTGTCG CCCCATTGCC GTCCTACCGG AACATTCCAT GGCCATCCTG 
GATGTGATGA TCTATCAGCC CCTGGGGCAA ATATTCAGCT TCTCCAAAGA CTCTGTGCTA 
AAGGTTTGGG ACATCCCCTC ACAGACGTGC CTGCACACCC TGGTGCTGAA GTTCCCCTGT 
ATCCAGCCCG GCAGGATCCA AGAGCAGGGC AGCTTCCCCT TCCTGCTGGT ACCCACCAAC 
CCCAGCCGCT TACTGGTCTG CTACTCCGAC TATATTGGCA TTCTGAGACT GGCACGGGCT 
GAGCCCACGG AGGGCACTTT CTTCACACAC AGGGCACCTG TTACCTGCCT CCTCTACAGC 
AGCTTCTGCC ATCTGATAGT TACGGGCAGC GAAGATTCCA CGGTTGCCGT GTGGGATGTG 
GAGACCGGCG CCAAATCTCT TCTACTCGGC AACGCGCACG GCCAGGAGGA AGTCACTTGC 
ATGGCGCTCG ACAAAGCGCA GAGGAGGCTC ATCACTGGGG CGAGGAACGG CACCATAAAG 
ATCTGGAACA TTCAGAACGG GCACAACCTG CACCAACTGG AGCCGGTGGA GGATGCGGAG 
ATCACTTGCA TCGTGGCCTC GCAGGAGAAT GTGTTCCTCA CTGTCGGCTG GAACCGGAAA 
ATTGTCACTT ACGACATCCC CAAAACCAAT GAGGTGTATG TGCCGGCCAA TCACTCGTGG 
AAAGGAGGGC AACTTCACCA GGAGGATATC CTGACTGCTG ATTTCTGCCC CTCCCTGGGG 
CTTCTTGCTA CAGCCAGTTT TGATGGAGAG ATCATTCTGT GGAACGCGGA GACTCAGAGA 
CTGTATCTGT ATCTGCGGCA GTGTCCCCAA GCAAGGTCTC CGGCACCGGT GGATAAACTC 
TTCTTCCTCC AGAAACGTTC GGCAAAGAGA GAAGCCGCCA TTCTGGTCAG TTCGGAGGCG 
GGGACCCTGC GTTGGTGGCG AGTGACCAAT GGTGGGCAGG AATGGGGGAG TTTCTATGCC 
CAGGATGAGG CAGACTGCTC TGTATTGGGG TTAGCAAGTG ACAAGGCAAA CAGGGTTCTG 
GTATCGGGAG ACACCCTGGG ATTGGTCCAG GTGTGGGACA TTTCCGCCTA TGGGCTACAG 
GCCGCCGCCC AGACCTACAC CTGTAGGCCA CCCCTACTTT ACTCTTGGGG GGCCCATGAC 
AGCGCCGTAG TCAGTGTGGA ACATTTCTTG TACGACTCGG AACCATTCAT TGCCAGCGGC 
TCCTCCGACA AAACGGCCAA ACTGTGGACA GTGGACGGGA AGCAAGTGGG AACGTTCGGC 
CAATCCAAAT GCTGGAAACT AAGTGACCCC ACCTCTTACC TGGACAGCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

efcab8 ( XM_012954181.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809635.1 Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa36144
Clone ID Related Accession (Same CDS sequence)	XM_012954181.2
Accession Version	XM_012954181.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	WD repeat-containing protein 49-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGCA CAGTGAGGAG GAGAAGAAGA GCCAAGGCAC AGGGGCTTCA AAAATGTTCT 
GGGCAAAAGG TGAAGTTCTG CAATGAGGAA AGCATCTTAA GCTTCGATCG CAATGAAAAC 
CTGCGCTTGG AGGAGAAGTT CCGTTTGGAT GACCTGCGCC TCCTGCAGGA AGCCTTCGCT 
AAGCACAATT CCGACGGCAA AGAGACCAAT TGCAAAGAGG AGTCCGACGA TCTCCGAGCG 
ACGAGGCGCA GAGCGAAACA GGAGGCGCGG CCTGCGGGAA ACATGACCCT CGATGAATTC 
CGCCGGGTTC TCTGCGACCT CTTCGGGTCG GAATGTTTCG ACGACGACAT GGAACTTCTG 
TTTAACGAGG TGGACACCTC CTGTGACGGC TACGTAGACT GGGGGGAGTT CTGCACTTAC 
CTGCTGCTGC AATACAGGAA GCGAGACTAC AGGATGATTG GCAGGGTGAC CTTCCTGGGA 
CAGCCAGTCA TACGGCTTTG CACTCGCAAC AAGCAAGAGC CAACCAGCAG GATCCTCGCC 
ATCAGCTCCC CCCCTCCCAT GTGGCTCCTC AGTGTGAGCA AAACCGGCGT CCTCACTACG 
TGGGATTTGG CCCTGCACCC CCAGAAATCC TACGAGGTTG CTGCTGAATA CCCAGACTCC 
CAAGCCGGGA AGAGGAGGTT CAAGTGCTGG GTAACGGACG CTGTGCATCT GCCCAACGCT 
AATAAAATCG CCATCGCCAC CACCAGCCGT GACATCCACT TCTTTGACGT CTCCACCATG 
AACTTGTTTG AGGAGTTTCA CCTGTTTGCT CTGAATCACG TTCCCACAAC CTTCTCATAT 
TGGTACAACA AACAGTCCCC AGAGAGTCGG TCCCTGCTGT TGTGGGGAGG AGATAATGGG 
GCGGTGAATG TTCTTTGGCT GCTCCAACCC CAGACGGGGG TATTTGAGAA ACCATTTAGC 
CAGCATCCGG GCCCTACTAA AATCTACATG CAGGAGCTGA AAGATCACAG CAAACTGCTG 
AGCTACCAGG TTATCCCCGA CGTTCACCCC GAGGCCATCA CCAAACTCAT ATACTGCCCG 
GAGAAGCAAC TGATCATTAC ATCATCGGGA AGCGCCCAAT CCTCGCTGCT CATTATGGAC 
ATTAACCAGA AGGGAAAGGT TTATACGTGG AAGATTAACA AGGGGGTGCA GTGTTTCGAC 
TACTGCCGGG CCTTGAATCT GCTGGTAACT GGGGGGTTCG ACCACAAAGT GCGACTATGG 
AATCAATACG TCACGTGTCG CCCCATTGCC GTCCTACCGG AACATTCCAT GGCCATCCTG 
GATGTGATGA TCTATCAGCC CCTGGGGCAA ATATTCAGCT TCTCCAAAGA CTCTGTGCTA 
AAGGTTTGGG ACATCCCCTC ACAGACGTGC CTGCACACCC TGGTGCTGAA GTTCCCCTGT 
ATCCAGCCCG GCAGGATCCA AGAGCAGGGC AGCTTCCCCT TCCTGCTGGT ACCCACCAAC 
CCCAGCCGCT TACTGGTCTG CTACTCCGAC TATATTGGCA TTCTGAGACT GGCACGGGCT 
GAGCCCACGG AGGGCACTTT CTTCACACAC AGGGCACCTG TTACCTGCCT CCTCTACAGC 
AGCTTCTGCC ATCTGATAGT TACGGGCAGC GAAGATTCCA CGGTTGCCGT GTGGGATGTG 
GAGACCGGCG CCAAATCTCT TCTACTCGGC AACGCGCACG GCCAGGAGGA AGTCACTTGC 
ATGGCGCTCG ACAAAGCGCA GAGGAGGCTC ATCACTGGGG CGAGGAACGG CACCATAAAG 
ATCTGGAACA TTCAGAACGG GCACAACCTG CACCAACTGG AGCCGGTGGA GGATGCGGAG 
ATCACTTGCA TCGTGGCCTC GCAGGAGAAT GTGTTCCTCA CTGTCGGCTG GAACCGGAAA 
ATTGTCACTT ACGACATCCC CAAAACCAAT GAGGTGTATG TGCCGGCCAA TCACTCGTGG 
AAAGGAGGGC AACTTCACCA GGAGGATATC CTGACTGCTG ATTTCTGCCC CTCCCTGGGG 
CTTCTTGCTA CAGCCAGTTT TGATGGAGAG ATCATTCTGT GGAACGCGGA GACTCAGAGA 
CTGTATCTGT ATCTGCGGCA GTGTCCCCAA GCAAGGTCTC CGGCACCGGT GGATAAACTC 
TTCTTCCTCC AGAAACGTTC GGCAAAGAGA GAAGCCGCCA TTCTGGTCAG TTCGGAGGCG 
GGGACCCTGC GTTGGTGGCG AGTGACCAAT GGTGGGCAGG AATGGGGGAG TTTCTATGCC 
CAGGATGAGG CAGACTGCTC TGTATTGGGG TTAGCAAGTG ACAAGGCAAA CAGGGTTCTG 
GTATCGGGAG ACACCCTGGG ATTGGTCCAG GTGTGGGACA TTTCCGCCTA TGGGCTACAG 
GCCGCCGCCC AGACCTACAC CTGTAGGCCA CCCCTACTTT ACTCTTGGGG GGCCCATGAC 
AGCGCCGTAG TCAGTGTGGA ACATTTCTTG TACGACTCGG AACCATTCAT TGCCAGCGGC 
TCCTCCGACA AAACGGCCAA ACTGTGGACA GTGGACGGGA AGCAAGTGGG AACGTTCGGC 
CAATCCAAAT GCTGGAAACT AAGTGACCCC ACCTCTTACC TGGACAGCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809635.1
CDS	116..2749
Translation	MECTVRRRRRAKAQGLQKCSGQKVKFCNEESILSFDRNENLRLEEKFRLDDLRLLQEAFAKHNSDGKETNCKEESDDLRATRRRAKQEARPAGNMTLDEFRRVLCDLFGSECFDDDMELLFNEVDTSCDGYVDWGEFCTYLLLQYRKRDYRMIGRVTFLGQPVIRLCTRNKQEPTSRILAISSPPPMWLLSVSKTGVLTTWDLALHPQKSYEVAAEYPDSQAGKRRFKCWVTDAVHLPNANKIAIATTSRDIHFFDVSTMNLFEEFHLFALNHVPTTFSYWYNKQSPESRSLLLWGGDNGAVNVLWLLQPQTGVFEKPFSQHPGPTKIYMQELKDHSKLLSYQVIPDVHPEAITKLIYCPEKQLIITSSGSAQSSLLIMDINQKGKVYTWKINKGVQCFDYCRALNLLVTGGFDHKVRLWNQYVTCRPIAVLPEHSMAILDVMIYQPLGQIFSFSKDSVLKVWDIPSQTCLHTLVLKFPCIQPGRIQEQGSFPFLLVPTNPSRLLVCYSDYIGILRLARAEPTEGTFFTHRAPVTCLLYSSFCHLIVTGSEDSTVAVWDVETGAKSLLLGNAHGQEEVTCMALDKAQRRLITGARNGTIKIWNIQNGHNLHQLEPVEDAEITCIVASQENVFLTVGWNRKIVTYDIPKTNEVYVPANHSWKGGQLHQEDILTADFCPSLGLLATASFDGEIILWNAETQRLYLYLRQCPQARSPAPVDKLFFLQKRSAKREAAILVSSEAGTLRWWRVTNGGQEWGSFYAQDEADCSVLGLASDKANRVLVSGDTLGLVQVWDISAYGLQAAAQTYTCRPPLLYSWGAHDSAVVSVEHFLYDSEPFIASGSSDKTAKLWTVDGKQVGTFGQSKCWKLSDPTSYLDSR

Target ORF information:

RefSeq Version	XM_012954181.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954181.2

ORF Insert Sequence:

ATGGAGTGCA CAGTGAGGAG GAGAAGAAGA GCCAAGGCAC AGGGGCTTCA AAAATGTTCT 
GGGCAAAAGG TGAAGTTCTG CAATGAGGAA AGCATCTTAA GCTTCGATCG CAATGAAAAC 
CTGCGCTTGG AGGAGAAGTT CCGTTTGGAT GACCTGCGCC TCCTGCAGGA AGCCTTCGCT 
AAGCACAATT CCGACGGCAA AGAGACCAAT TGCAAAGAGG AGTCCGACGA TCTCCGAGCG 
ACGAGGCGCA GAGCGAAACA GGAGGCGCGG CCTGCGGGAA ACATGACCCT CGATGAATTC 
CGCCGGGTTC TCTGCGACCT CTTCGGGTCG GAATGTTTCG ACGACGACAT GGAACTTCTG 
TTTAACGAGG TGGACACCTC CTGTGACGGC TACGTAGACT GGGGGGAGTT CTGCACTTAC 
CTGCTGCTGC AATACAGGAA GCGAGACTAC AGGATGATTG GCAGGGTGAC CTTCCTGGGA 
CAGCCAGTCA TACGGCTTTG CACTCGCAAC AAGCAAGAGC CAACCAGCAG GATCCTCGCC 
ATCAGCTCCC CCCCTCCCAT GTGGCTCCTC AGTGTGAGCA AAACCGGCGT CCTCACTACG 
TGGGATTTGG CCCTGCACCC CCAGAAATCC TACGAGGTTG CTGCTGAATA CCCAGACTCC 
CAAGCCGGGA AGAGGAGGTT CAAGTGCTGG GTAACGGACG CTGTGCATCT GCCCAACGCT 
AATAAAATCG CCATCGCCAC CACCAGCCGT GACATCCACT TCTTTGACGT CTCCACCATG 
AACTTGTTTG AGGAGTTTCA CCTGTTTGCT CTGAATCACG TTCCCACAAC CTTCTCATAT 
TGGTACAACA AACAGTCCCC AGAGAGTCGG TCCCTGCTGT TGTGGGGAGG AGATAATGGG 
GCGGTGAATG TTCTTTGGCT GCTCCAACCC CAGACGGGGG TATTTGAGAA ACCATTTAGC 
CAGCATCCGG GCCCTACTAA AATCTACATG CAGGAGCTGA AAGATCACAG CAAACTGCTG 
AGCTACCAGG TTATCCCCGA CGTTCACCCC GAGGCCATCA CCAAACTCAT ATACTGCCCG 
GAGAAGCAAC TGATCATTAC ATCATCGGGA AGCGCCCAAT CCTCGCTGCT CATTATGGAC 
ATTAACCAGA AGGGAAAGGT TTATACGTGG AAGATTAACA AGGGGGTGCA GTGTTTCGAC 
TACTGCCGGG CCTTGAATCT GCTGGTAACT GGGGGGTTCG ACCACAAAGT GCGACTATGG 
AATCAATACG TCACGTGTCG CCCCATTGCC GTCCTACCGG AACATTCCAT GGCCATCCTG 
GATGTGATGA TCTATCAGCC CCTGGGGCAA ATATTCAGCT TCTCCAAAGA CTCTGTGCTA 
AAGGTTTGGG ACATCCCCTC ACAGACGTGC CTGCACACCC TGGTGCTGAA GTTCCCCTGT 
ATCCAGCCCG GCAGGATCCA AGAGCAGGGC AGCTTCCCCT TCCTGCTGGT ACCCACCAAC 
CCCAGCCGCT TACTGGTCTG CTACTCCGAC TATATTGGCA TTCTGAGACT GGCACGGGCT 
GAGCCCACGG AGGGCACTTT CTTCACACAC AGGGCACCTG TTACCTGCCT CCTCTACAGC 
AGCTTCTGCC ATCTGATAGT TACGGGCAGC GAAGATTCCA CGGTTGCCGT GTGGGATGTG 
GAGACCGGCG CCAAATCTCT TCTACTCGGC AACGCGCACG GCCAGGAGGA AGTCACTTGC 
ATGGCGCTCG ACAAAGCGCA GAGGAGGCTC ATCACTGGGG CGAGGAACGG CACCATAAAG 
ATCTGGAACA TTCAGAACGG GCACAACCTG CACCAACTGG AGCCGGTGGA GGATGCGGAG 
ATCACTTGCA TCGTGGCCTC GCAGGAGAAT GTGTTCCTCA CTGTCGGCTG GAACCGGAAA 
ATTGTCACTT ACGACATCCC CAAAACCAAT GAGGTGTATG TGCCGGCCAA TCACTCGTGG 
AAAGGAGGGC AACTTCACCA GGAGGATATC CTGACTGCTG ATTTCTGCCC CTCCCTGGGG 
CTTCTTGCTA CAGCCAGTTT TGATGGAGAG ATCATTCTGT GGAACGCGGA GACTCAGAGA 
CTGTATCTGT ATCTGCGGCA GTGTCCCCAA GCAAGGTCTC CGGCACCGGT GGATAAACTC 
TTCTTCCTCC AGAAACGTTC GGCAAAGAGA GAAGCCGCCA TTCTGGTCAG TTCGGAGGCG 
GGGACCCTGC GTTGGTGGCG AGTGACCAAT GGTGGGCAGG AATGGGGGAG TTTCTATGCC 
CAGGATGAGG CAGACTGCTC TGTATTGGGG TTAGCAAGTG ACAAGGCAAA CAGGGTTCTG 
GTATCGGGAG ACACCCTGGG ATTGGTCCAG GTGTGGGACA TTTCCGCCTA TGGGCTACAG 
GCCGCCGCCC AGACCTACAC CTGTAGGCCA CCCCTACTTT ACTCTTGGGG GGCCCATGAC 
AGCGCCGTAG TCAGTGTGGA ACATTTCTTG TACGACTCGG AACCATTCAT TGCCAGCGGC 
TCCTCCGACA AAACGGCCAA ACTGTGGACA GTGGACGGGA AGCAAGTGGG AACGTTCGGC 
CAATCCAAAT GCTGGAAACT AAGTGACCCC ACCTCTTACC TGGACAGCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

efcab8 ( XM_012954182.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809636.1 Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X3, mRNA.

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CloneID	OXa36145
Clone ID Related Accession (Same CDS sequence)	XM_012954182.2
Accession Version	XM_012954182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	WD repeat-containing protein 49-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTACTA AAAGTAACGG ATTCTGTGTC TATGAAGAAG AAATTCCATC GAAAGTAAAA 
CGCTTCGATC GCAATGAAAA CCTGCGCTTG GAGGAGAAGT TCCGTTTGGA TGACCTGCGC 
CTCCTGCAGG AAGCCTTCGC TAAGCACAAT TCCGACGGCA AAGAGACCAA TTGCAAAGAG 
GAGTCCGACG ATCTCCGAGC GACGAGGCGC AGAGCGAAAC AGGAGGCGCG GCCTGCGGGA 
AACATGACCC TCGATGAATT CCGCCGGGTT CTCTGCGACC TCTTCGGGTC GGAATGTTTC 
GACGACGACA TGGAACTTCT GTTTAACGAG GTGGACACCT CCTGTGACGG CTACGTAGAC 
TGGGGGGAGT TCTGCACTTA CCTGCTGCTG CAATACAGGA AGCGAGACTA CAGGATGATT 
GGCAGGGTGA CCTTCCTGGG ACAGCCAGTC ATACGGCTTT GCACTCGCAA CAAGCAAGAG 
CCAACCAGCA GGATCCTCGC CATCAGCTCC CCCCCTCCCA TGTGGCTCCT CAGTGTGAGC 
AAAACCGGCG TCCTCACTAC GTGGGATTTG GCCCTGCACC CCCAGAAATC CTACGAGGTT 
GCTGCTGAAT ACCCAGACTC CCAAGCCGGG AAGAGGAGGT TCAAGTGCTG GGTAACGGAC 
GCTGTGCATC TGCCCAACGC TAATAAAATC GCCATCGCCA CCACCAGCCG TGACATCCAC 
TTCTTTGACG TCTCCACCAT GAACTTGTTT GAGGAGTTTC ACCTGTTTGC TCTGAATCAC 
GTTCCCACAA CCTTCTCATA TTGGTACAAC AAACAGTCCC CAGAGAGTCG GTCCCTGCTG 
TTGTGGGGAG GAGATAATGG GGCGGTGAAT GTTCTTTGGC TGCTCCAACC CCAGACGGGG 
GTATTTGAGA AACCATTTAG CCAGCATCCG GGCCCTACTA AAATCTACAT GCAGGAGCTG 
AAAGATCACA GCAAACTGCT GAGCTACCAG GTTATCCCCG ACGTTCACCC CGAGGCCATC 
ACCAAACTCA TATACTGCCC GGAGAAGCAA CTGATCATTA CATCATCGGG AAGCGCCCAA 
TCCTCGCTGC TCATTATGGA CATTAACCAG AAGGGAAAGG TTTATACGTG GAAGATTAAC 
AAGGGGGTGC AGTGTTTCGA CTACTGCCGG GCCTTGAATC TGCTGGTAAC TGGGGGGTTC 
GACCACAAAG TGCGACTATG GAATCAATAC GTCACGTGTC GCCCCATTGC CGTCCTACCG 
GAACATTCCA TGGCCATCCT GGATGTGATG ATCTATCAGC CCCTGGGGCA AATATTCAGC 
TTCTCCAAAG ACTCTGTGCT AAAGGTTTGG GACATCCCCT CACAGACGTG CCTGCACACC 
CTGGTGCTGA AGTTCCCCTG TATCCAGCCC GGCAGGATCC AAGAGCAGGG CAGCTTCCCC 
TTCCTGCTGG TACCCACCAA CCCCAGCCGC TTACTGGTCT GCTACTCCGA CTATATTGGC 
ATTCTGAGAC TGGCACGGGC TGAGCCCACG GAGGGCACTT TCTTCACACA CAGGGCACCT 
GTTACCTGCC TCCTCTACAG CAGCTTCTGC CATCTGATAG TTACGGGCAG CGAAGATTCC 
ACGGTTGCCG TGTGGGATGT GGAGACCGGC GCCAAATCTC TTCTACTCGG CAACGCGCAC 
GGCCAGGAGG AAGTCACTTG CATGGCGCTC GACAAAGCGC AGAGGAGGCT CATCACTGGG 
GCGAGGAACG GCACCATAAA GATCTGGAAC ATTCAGAACG GGCACAACCT GCACCAACTG 
GAGCCGGTGG AGGATGCGGA GATCACTTGC ATCGTGGCCT CGCAGGAGAA TGTGTTCCTC 
ACTGTCGGCT GGAACCGGAA AATTGTCACT TACGACATCC CCAAAACCAA TGAGGTGTAT 
GTGCCGGCCA ATCACTCGTG GAAAGGAGGG CAACTTCACC AGGAGGATAT CCTGACTGCT 
GATTTCTGCC CCTCCCTGGG GCTTCTTGCT ACAGCCAGTT TTGATGGAGA GATCATTCTG 
TGGAACGCGG AGACTCAGAG ACTGTATCTG TATCTGCGGC AGTGTCCCCA AGCAAGGTCT 
CCGGCACCGG TGGATAAACT CTTCTTCCTC CAGAAACGTT CGGCAAAGAG AGAAGCCGCC 
ATTCTGGTCA GTTCGGAGGC GGGGACCCTG CGTTGGTGGC GAGTGACCAA TGGTGGGCAG 
GAATGGGGGA GTTTCTATGC CCAGGATGAG GCAGACTGCT CTGTATTGGG GTTAGCAAGT 
GACAAGGCAA ACAGGGTTCT GGTATCGGGA GACACCCTGG GATTGGTCCA GGTGTGGGAC 
ATTTCCGCCT ATGGGCTACA GGCCGCCGCC CAGACCTACA CCTGTAGGCC ACCCCTACTT 
TACTCTTGGG GGGCCCATGA CAGCGCCGTA GTCAGTGTGG AACATTTCTT GTACGACTCG 
GAACCATTCA TTGCCAGCGG CTCCTCCGAC AAAACGGCCA AACTGTGGAC AGTGGACGGG 
AAGCAAGTGG GAACGTTCGG CCAATCCAAA TGCTGGAAAC TAAGTGACCC CACCTCTTAC 
CTGGACAGCA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809636.1
CDS	81..2675
Translation	MGTKSNGFCVYEEEIPSKVKRFDRNENLRLEEKFRLDDLRLLQEAFAKHNSDGKETNCKEESDDLRATRRRAKQEARPAGNMTLDEFRRVLCDLFGSECFDDDMELLFNEVDTSCDGYVDWGEFCTYLLLQYRKRDYRMIGRVTFLGQPVIRLCTRNKQEPTSRILAISSPPPMWLLSVSKTGVLTTWDLALHPQKSYEVAAEYPDSQAGKRRFKCWVTDAVHLPNANKIAIATTSRDIHFFDVSTMNLFEEFHLFALNHVPTTFSYWYNKQSPESRSLLLWGGDNGAVNVLWLLQPQTGVFEKPFSQHPGPTKIYMQELKDHSKLLSYQVIPDVHPEAITKLIYCPEKQLIITSSGSAQSSLLIMDINQKGKVYTWKINKGVQCFDYCRALNLLVTGGFDHKVRLWNQYVTCRPIAVLPEHSMAILDVMIYQPLGQIFSFSKDSVLKVWDIPSQTCLHTLVLKFPCIQPGRIQEQGSFPFLLVPTNPSRLLVCYSDYIGILRLARAEPTEGTFFTHRAPVTCLLYSSFCHLIVTGSEDSTVAVWDVETGAKSLLLGNAHGQEEVTCMALDKAQRRLITGARNGTIKIWNIQNGHNLHQLEPVEDAEITCIVASQENVFLTVGWNRKIVTYDIPKTNEVYVPANHSWKGGQLHQEDILTADFCPSLGLLATASFDGEIILWNAETQRLYLYLRQCPQARSPAPVDKLFFLQKRSAKREAAILVSSEAGTLRWWRVTNGGQEWGSFYAQDEADCSVLGLASDKANRVLVSGDTLGLVQVWDISAYGLQAAAQTYTCRPPLLYSWGAHDSAVVSVEHFLYDSEPFIASGSSDKTAKLWTVDGKQVGTFGQSKCWKLSDPTSYLDSR

Target ORF information:

RefSeq Version	XM_012954182.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954182.2

ORF Insert Sequence:

ATGGGTACTA AAAGTAACGG ATTCTGTGTC TATGAAGAAG AAATTCCATC GAAAGTAAAA 
CGCTTCGATC GCAATGAAAA CCTGCGCTTG GAGGAGAAGT TCCGTTTGGA TGACCTGCGC 
CTCCTGCAGG AAGCCTTCGC TAAGCACAAT TCCGACGGCA AAGAGACCAA TTGCAAAGAG 
GAGTCCGACG ATCTCCGAGC GACGAGGCGC AGAGCGAAAC AGGAGGCGCG GCCTGCGGGA 
AACATGACCC TCGATGAATT CCGCCGGGTT CTCTGCGACC TCTTCGGGTC GGAATGTTTC 
GACGACGACA TGGAACTTCT GTTTAACGAG GTGGACACCT CCTGTGACGG CTACGTAGAC 
TGGGGGGAGT TCTGCACTTA CCTGCTGCTG CAATACAGGA AGCGAGACTA CAGGATGATT 
GGCAGGGTGA CCTTCCTGGG ACAGCCAGTC ATACGGCTTT GCACTCGCAA CAAGCAAGAG 
CCAACCAGCA GGATCCTCGC CATCAGCTCC CCCCCTCCCA TGTGGCTCCT CAGTGTGAGC 
AAAACCGGCG TCCTCACTAC GTGGGATTTG GCCCTGCACC CCCAGAAATC CTACGAGGTT 
GCTGCTGAAT ACCCAGACTC CCAAGCCGGG AAGAGGAGGT TCAAGTGCTG GGTAACGGAC 
GCTGTGCATC TGCCCAACGC TAATAAAATC GCCATCGCCA CCACCAGCCG TGACATCCAC 
TTCTTTGACG TCTCCACCAT GAACTTGTTT GAGGAGTTTC ACCTGTTTGC TCTGAATCAC 
GTTCCCACAA CCTTCTCATA TTGGTACAAC AAACAGTCCC CAGAGAGTCG GTCCCTGCTG 
TTGTGGGGAG GAGATAATGG GGCGGTGAAT GTTCTTTGGC TGCTCCAACC CCAGACGGGG 
GTATTTGAGA AACCATTTAG CCAGCATCCG GGCCCTACTA AAATCTACAT GCAGGAGCTG 
AAAGATCACA GCAAACTGCT GAGCTACCAG GTTATCCCCG ACGTTCACCC CGAGGCCATC 
ACCAAACTCA TATACTGCCC GGAGAAGCAA CTGATCATTA CATCATCGGG AAGCGCCCAA 
TCCTCGCTGC TCATTATGGA CATTAACCAG AAGGGAAAGG TTTATACGTG GAAGATTAAC 
AAGGGGGTGC AGTGTTTCGA CTACTGCCGG GCCTTGAATC TGCTGGTAAC TGGGGGGTTC 
GACCACAAAG TGCGACTATG GAATCAATAC GTCACGTGTC GCCCCATTGC CGTCCTACCG 
GAACATTCCA TGGCCATCCT GGATGTGATG ATCTATCAGC CCCTGGGGCA AATATTCAGC 
TTCTCCAAAG ACTCTGTGCT AAAGGTTTGG GACATCCCCT CACAGACGTG CCTGCACACC 
CTGGTGCTGA AGTTCCCCTG TATCCAGCCC GGCAGGATCC AAGAGCAGGG CAGCTTCCCC 
TTCCTGCTGG TACCCACCAA CCCCAGCCGC TTACTGGTCT GCTACTCCGA CTATATTGGC 
ATTCTGAGAC TGGCACGGGC TGAGCCCACG GAGGGCACTT TCTTCACACA CAGGGCACCT 
GTTACCTGCC TCCTCTACAG CAGCTTCTGC CATCTGATAG TTACGGGCAG CGAAGATTCC 
ACGGTTGCCG TGTGGGATGT GGAGACCGGC GCCAAATCTC TTCTACTCGG CAACGCGCAC 
GGCCAGGAGG AAGTCACTTG CATGGCGCTC GACAAAGCGC AGAGGAGGCT CATCACTGGG 
GCGAGGAACG GCACCATAAA GATCTGGAAC ATTCAGAACG GGCACAACCT GCACCAACTG 
GAGCCGGTGG AGGATGCGGA GATCACTTGC ATCGTGGCCT CGCAGGAGAA TGTGTTCCTC 
ACTGTCGGCT GGAACCGGAA AATTGTCACT TACGACATCC CCAAAACCAA TGAGGTGTAT 
GTGCCGGCCA ATCACTCGTG GAAAGGAGGG CAACTTCACC AGGAGGATAT CCTGACTGCT 
GATTTCTGCC CCTCCCTGGG GCTTCTTGCT ACAGCCAGTT TTGATGGAGA GATCATTCTG 
TGGAACGCGG AGACTCAGAG ACTGTATCTG TATCTGCGGC AGTGTCCCCA AGCAAGGTCT 
CCGGCACCGG TGGATAAACT CTTCTTCCTC CAGAAACGTT CGGCAAAGAG AGAAGCCGCC 
ATTCTGGTCA GTTCGGAGGC GGGGACCCTG CGTTGGTGGC GAGTGACCAA TGGTGGGCAG 
GAATGGGGGA GTTTCTATGC CCAGGATGAG GCAGACTGCT CTGTATTGGG GTTAGCAAGT 
GACAAGGCAA ACAGGGTTCT GGTATCGGGA GACACCCTGG GATTGGTCCA GGTGTGGGAC 
ATTTCCGCCT ATGGGCTACA GGCCGCCGCC CAGACCTACA CCTGTAGGCC ACCCCTACTT 
TACTCTTGGG GGGCCCATGA CAGCGCCGTA GTCAGTGTGG AACATTTCTT GTACGACTCG 
GAACCATTCA TTGCCAGCGG CTCCTCCGAC AAAACGGCCA AACTGTGGAC AGTGGACGGG 
AAGCAAGTGG GAACGTTCGG CCAATCCAAA TGCTGGAAAC TAAGTGACCC CACCTCTTAC 
CTGGACAGCA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

efcab8 ( XM_031894727.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750587.1 Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa50838
Clone ID Related Accession (Same CDS sequence)	XM_031894727.1 , XM_031894726.1
Accession Version	XM_031894727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	WD repeat-containing protein on Y chromosome isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTTC CGACTCTTCT TCAGAAACGT GCCAATGCCC TACAGAGACA ACTCCTGTCT 
GTCACTCTAC TTCAAGAGCT AGAACACTTC CTAAAGAAGC AGGGGCTTCA AAAATGTTCT 
GGGCAAAAGG TGAAGTTCTG CAATGAGGAA AGCATCTTAA GCTTCGATCG CAATGAAAAC 
CTGCGCTTGG AGGAGAAGTT CCGTTTGGAT GACCTGCGCC TCCTGCAGGA AGCCTTCGCT 
AAGCACAATT CCGACGGCAA AGAGACCAAT TGCAAAGAGG AGTCCGACGA TCTCCGAGCG 
ACGAGGCGCA GAGCGAAACA GGAGGCGCGG CCTGCGGGAA ACATGACCCT CGATGAATTC 
CGCCGGGTTC TCTGCGACCT CTTCGGGTCG GAATGTTTCG ACGACGACAT GGAACTTCTG 
TTTAACGAGG TGGACACCTC CTGTGACGGC TACGTAGACT GGGGGGAGTT CTGCACTTAC 
CTGCTGCTGC AATACAGGAA GCGAGACTAC AGGATGATTG GCAGGGTGAC CTTCCTGGGA 
CAGCCAGTCA TACGGCTTTG CACTCGCAAC AAGCAAGAGC CAACCAGCAG GATCCTCGCC 
ATCAGCTCCC CCCCTCCCAT GTGGCTCCTC AGTGTGAGCA AAACCGGCGT CCTCACTACG 
TGGGATTTGG CCCTGCACCC CCAGAAATCC TACGAGGTTG CTGCTGAATA CCCAGACTCC 
CAAGCCGGGA AGAGGAGGTT CAAGTGCTGG GTAACGGACG CTGTGCATCT GCCCAACGCT 
AATAAAATCG CCATCGCCAC CACCAGCCGT GACATCCACT TCTTTGACGT CTCCACCATG 
AACTTGTTTG AGGAGTTTCA CCTGTTTGCT CTGAATCACG TTCCCACAAC CTTCTCATAT 
TGGTACAACA AACAGTCCCC AGAGAGTCGG TCCCTGCTGT TGTGGGGAGG AGATAATGGG 
GCGATGAATG TTCTTTGGCT GCTCCAACCC CAGACGGGGG TATTTGAGAA ACCATTTAGC 
CAGCATCCGG GCCCTACTAA AATCTACATG CAGGAGCTGA AAGATCACAG CAAACTGCTG 
AGCTACCAGG TTATCCCCGA CGTTCACCCC GAGGCCATCA CCAAACTCAT ATACTGCCCG 
GAGAAGCAAC TGATCATTAC ATCATCGGGA AGCGCCCAAT CCTCGCTGCT CATTATGGAC 
ATTAACCAGA AGGGAAAGGT TTATACGTGG AAGATTAACA AGGGGGTGCA GTGTTTCGAC 
TACTGCCGGG CCTTGAATCT GCTGGTAACT GGGGGGTTCG ACCACAAAGT GCGACTATGG 
AATCAATACG TCACGTGTCG CCCCATTGCC GTCCTACCGG AACATTCCAT GGCCATCCTG 
GATGTGATGA TCTATCAGCC CCTGGGGCAA ATATTCAGCT TCTCCAAAGA CTCTGTGCTA 
AAGGTTTGGG ACATCCCCTC ACAGACGTGC CTGCACACCC TGGTGCTGAA GTTCCCCTGT 
ATCCAGCCCG GCAGGATCCA AGAGCAGGGC AGCTTCCCCT TCCTGCTGGT ACCCACCAAC 
CCCAGCCGCT TACTGGTCTG CTACTCCGAC TATATTGGCA TTCTGAGACT GGCACGGGCT 
GAGCCCACGG AGGGCACTTT CTTCACACAC AGGGCACCTG TTACCTGCCT CCTCTACAGC 
AGCTTCTGCC ATCTGATAGT TACGGGCAGC GAAGATTCCA CGGTTGCCGT GTGGGATGTG 
GAGACCGGCG CCAAATCTCT TCTACTCGGC AACGCGCACG GCCAGGAGGA AGTCACTTGC 
ATGGCGCTCG ACAAAGCGCA GAGGAGGCTC ATCACTGGGG CGAGGAACGG CACCATAAAG 
ATCTGGAACA TTCAGAACGG GCACAACCTG CACCAACTGG AGCCGGTGGA GGATGCGGAG 
ATCACTTGCA TCGTGGCCTC GCAGGAGAAT GTGTTCCTCA CTGTCGGCTG GAACCGGAAA 
ATTGTCACTT ACGACATCCC CAAAACCAAT GAGGTGTATG TGCCGGCCAA TCACTCGTGG 
AAAGGAGGGC AACTTCACCA GGAGGATATC CTGACTGCTG ATTTCTGCCC CTCCCTGGGG 
CTTCTTGCTA CAGCCAGTTT TGATGGAGAG ATCATTCTGT GGAACGCGGA GACTCAGAGA 
CTGTATCTGT ATCTGCGGCA GTGTCCCCAA GCAAGGTCTC CGGCACCGGT GGATAAACTC 
TTCTTCCTCC AGAAACGTTC GGCAAAGAGA GAAGCCGCCA TTCTGGTCAG TTCGGAGGCG 
GGGACCCTGC GTTGGTGGCG AGTGACCAAT GGTGGGCAGG AATGGGGGAG TTTCTATGCC 
CAGGATGAGG CAGACTGCTC TGTATTGGGG TTAGCAAGTG ACAAGGCAAA CAGGGTTCTG 
GTATCGGGAG ACACCCTGGG ATTGGTCCAG GTGTGGGACA TTTCCGCCTA TGGGCTACAG 
GCCGCCGCCC AGACCTACAC CTGTAGGCCA CCCCTACTTT ACTCTTGGGG GGCCCATGAC 
AGCGCCGTAG TCAGTGTGGA ACATTTCTTG TACGACTCGG AACCATTCAT TGCCAGCGGC 
TCCTCCGACA AAACGGCCAA ACTGTGGACA GTGGACGGGA AGCAAGTGGG AACGTTCGGC 
CAATCCAAAT GCTGGAAACT AAGTGACCCC ACCTCTTACC TGGACAGCAG GTCGGAGGGA 
AGTGAAGGAA AGGGCCCCCG GGGCGCAGGA GGGCTGGAGA TCCTGCCGGG AGTAACTGAG 
ATACAGGAGG AGAATAGCGA GGGCAGAGCT CTAACACCCG CCCCCGAGGA TCTGCCTGAG 
ATACTGAGCC AAGAAATTCC TGACCTGGCA ACAGGATCAA GTTCCGAGGC CAGCTTGGGA 
AGTGCGCAGG AAGAGAGCAC AAACATGGCC GCCGCTAGGA AAGAGCGCAG GCAGCGCCTC 
ACTGACATCG ATGTGAATAA GTGCAACCGA TTTGGCCACA TCTGCTCCCC ATTCCAAGCC 
TTGATCACCC CTGCCGTACA GGAGATCAGC TTGCCCCCGG GCCTGCCCGT GATGATGAGG 
AGCAGCAGTG GTGCCAGCAG TGAGCGACAG GACTCAGGGA TTGGGGGCAG AATGGATCCA 
GGAGAATTCA ACCCACAAGG GAGTAAAAAC TCACAGACGC TACAAGAGAA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750587.1
CDS	927..4103
Translation	MPLPTLLQKRANALQRQLLSVTLLQELEHFLKKQGLQKCSGQKVKFCNEESILSFDRNENLRLEEKFRLDDLRLLQEAFAKHNSDGKETNCKEESDDLRATRRRAKQEARPAGNMTLDEFRRVLCDLFGSECFDDDMELLFNEVDTSCDGYVDWGEFCTYLLLQYRKRDYRMIGRVTFLGQPVIRLCTRNKQEPTSRILAISSPPPMWLLSVSKTGVLTTWDLALHPQKSYEVAAEYPDSQAGKRRFKCWVTDAVHLPNANKIAIATTSRDIHFFDVSTMNLFEEFHLFALNHVPTTFSYWYNKQSPESRSLLLWGGDNGAMNVLWLLQPQTGVFEKPFSQHPGPTKIYMQELKDHSKLLSYQVIPDVHPEAITKLIYCPEKQLIITSSGSAQSSLLIMDINQKGKVYTWKINKGVQCFDYCRALNLLVTGGFDHKVRLWNQYVTCRPIAVLPEHSMAILDVMIYQPLGQIFSFSKDSVLKVWDIPSQTCLHTLVLKFPCIQPGRIQEQGSFPFLLVPTNPSRLLVCYSDYIGILRLARAEPTEGTFFTHRAPVTCLLYSSFCHLIVTGSEDSTVAVWDVETGAKSLLLGNAHGQEEVTCMALDKAQRRLITGARNGTIKIWNIQNGHNLHQLEPVEDAEITCIVASQENVFLTVGWNRKIVTYDIPKTNEVYVPANHSWKGGQLHQEDILTADFCPSLGLLATASFDGEIILWNAETQRLYLYLRQCPQARSPAPVDKLFFLQKRSAKREAAILVSSEAGTLRWWRVTNGGQEWGSFYAQDEADCSVLGLASDKANRVLVSGDTLGLVQVWDISAYGLQAAAQTYTCRPPLLYSWGAHDSAVVSVEHFLYDSEPFIASGSSDKTAKLWTVDGKQVGTFGQSKCWKLSDPTSYLDSRSEGSEGKGPRGAGGLEILPGVTEIQEENSEGRALTPAPEDLPEILSQEIPDLATGSSSEASLGSAQEESTNMAAARKERRQRLTDIDVNKCNRFGHICSPFQALITPAVQEISLPPGLPVMMRSSSGASSERQDSGIGGRMDPGEFNPQGSKNSQTLQEKT

Target ORF information:

RefSeq Version	XM_031894727.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894727.1

ORF Insert Sequence:

ATGCCCCTTC CGACTCTTCT TCAGAAACGT GCCAATGCCC TACAGAGACA ACTCCTGTCT 
GTCACTCTAC TTCAAGAGCT AGAACACTTC CTAAAGAAGC AGGGGCTTCA AAAATGTTCT 
GGGCAAAAGG TGAAGTTCTG CAATGAGGAA AGCATCTTAA GCTTCGATCG CAATGAAAAC 
CTGCGCTTGG AGGAGAAGTT CCGTTTGGAT GACCTGCGCC TCCTGCAGGA AGCCTTCGCT 
AAGCACAATT CCGACGGCAA AGAGACCAAT TGCAAAGAGG AGTCCGACGA TCTCCGAGCG 
ACGAGGCGCA GAGCGAAACA GGAGGCGCGG CCTGCGGGAA ACATGACCCT CGATGAATTC 
CGCCGGGTTC TCTGCGACCT CTTCGGGTCG GAATGTTTCG ACGACGACAT GGAACTTCTG 
TTTAACGAGG TGGACACCTC CTGTGACGGC TACGTAGACT GGGGGGAGTT CTGCACTTAC 
CTGCTGCTGC AATACAGGAA GCGAGACTAC AGGATGATTG GCAGGGTGAC CTTCCTGGGA 
CAGCCAGTCA TACGGCTTTG CACTCGCAAC AAGCAAGAGC CAACCAGCAG GATCCTCGCC 
ATCAGCTCCC CCCCTCCCAT GTGGCTCCTC AGTGTGAGCA AAACCGGCGT CCTCACTACG 
TGGGATTTGG CCCTGCACCC CCAGAAATCC TACGAGGTTG CTGCTGAATA CCCAGACTCC 
CAAGCCGGGA AGAGGAGGTT CAAGTGCTGG GTAACGGACG CTGTGCATCT GCCCAACGCT 
AATAAAATCG CCATCGCCAC CACCAGCCGT GACATCCACT TCTTTGACGT CTCCACCATG 
AACTTGTTTG AGGAGTTTCA CCTGTTTGCT CTGAATCACG TTCCCACAAC CTTCTCATAT 
TGGTACAACA AACAGTCCCC AGAGAGTCGG TCCCTGCTGT TGTGGGGAGG AGATAATGGG 
GCGATGAATG TTCTTTGGCT GCTCCAACCC CAGACGGGGG TATTTGAGAA ACCATTTAGC 
CAGCATCCGG GCCCTACTAA AATCTACATG CAGGAGCTGA AAGATCACAG CAAACTGCTG 
AGCTACCAGG TTATCCCCGA CGTTCACCCC GAGGCCATCA CCAAACTCAT ATACTGCCCG 
GAGAAGCAAC TGATCATTAC ATCATCGGGA AGCGCCCAAT CCTCGCTGCT CATTATGGAC 
ATTAACCAGA AGGGAAAGGT TTATACGTGG AAGATTAACA AGGGGGTGCA GTGTTTCGAC 
TACTGCCGGG CCTTGAATCT GCTGGTAACT GGGGGGTTCG ACCACAAAGT GCGACTATGG 
AATCAATACG TCACGTGTCG CCCCATTGCC GTCCTACCGG AACATTCCAT GGCCATCCTG 
GATGTGATGA TCTATCAGCC CCTGGGGCAA ATATTCAGCT TCTCCAAAGA CTCTGTGCTA 
AAGGTTTGGG ACATCCCCTC ACAGACGTGC CTGCACACCC TGGTGCTGAA GTTCCCCTGT 
ATCCAGCCCG GCAGGATCCA AGAGCAGGGC AGCTTCCCCT TCCTGCTGGT ACCCACCAAC 
CCCAGCCGCT TACTGGTCTG CTACTCCGAC TATATTGGCA TTCTGAGACT GGCACGGGCT 
GAGCCCACGG AGGGCACTTT CTTCACACAC AGGGCACCTG TTACCTGCCT CCTCTACAGC 
AGCTTCTGCC ATCTGATAGT TACGGGCAGC GAAGATTCCA CGGTTGCCGT GTGGGATGTG 
GAGACCGGCG CCAAATCTCT TCTACTCGGC AACGCGCACG GCCAGGAGGA AGTCACTTGC 
ATGGCGCTCG ACAAAGCGCA GAGGAGGCTC ATCACTGGGG CGAGGAACGG CACCATAAAG 
ATCTGGAACA TTCAGAACGG GCACAACCTG CACCAACTGG AGCCGGTGGA GGATGCGGAG 
ATCACTTGCA TCGTGGCCTC GCAGGAGAAT GTGTTCCTCA CTGTCGGCTG GAACCGGAAA 
ATTGTCACTT ACGACATCCC CAAAACCAAT GAGGTGTATG TGCCGGCCAA TCACTCGTGG 
AAAGGAGGGC AACTTCACCA GGAGGATATC CTGACTGCTG ATTTCTGCCC CTCCCTGGGG 
CTTCTTGCTA CAGCCAGTTT TGATGGAGAG ATCATTCTGT GGAACGCGGA GACTCAGAGA 
CTGTATCTGT ATCTGCGGCA GTGTCCCCAA GCAAGGTCTC CGGCACCGGT GGATAAACTC 
TTCTTCCTCC AGAAACGTTC GGCAAAGAGA GAAGCCGCCA TTCTGGTCAG TTCGGAGGCG 
GGGACCCTGC GTTGGTGGCG AGTGACCAAT GGTGGGCAGG AATGGGGGAG TTTCTATGCC 
CAGGATGAGG CAGACTGCTC TGTATTGGGG TTAGCAAGTG ACAAGGCAAA CAGGGTTCTG 
GTATCGGGAG ACACCCTGGG ATTGGTCCAG GTGTGGGACA TTTCCGCCTA TGGGCTACAG 
GCCGCCGCCC AGACCTACAC CTGTAGGCCA CCCCTACTTT ACTCTTGGGG GGCCCATGAC 
AGCGCCGTAG TCAGTGTGGA ACATTTCTTG TACGACTCGG AACCATTCAT TGCCAGCGGC 
TCCTCCGACA AAACGGCCAA ACTGTGGACA GTGGACGGGA AGCAAGTGGG AACGTTCGGC 
CAATCCAAAT GCTGGAAACT AAGTGACCCC ACCTCTTACC TGGACAGCAG GTCGGAGGGA 
AGTGAAGGAA AGGGCCCCCG GGGCGCAGGA GGGCTGGAGA TCCTGCCGGG AGTAACTGAG 
ATACAGGAGG AGAATAGCGA GGGCAGAGCT CTAACACCCG CCCCCGAGGA TCTGCCTGAG 
ATACTGAGCC AAGAAATTCC TGACCTGGCA ACAGGATCAA GTTCCGAGGC CAGCTTGGGA 
AGTGCGCAGG AAGAGAGCAC AAACATGGCC GCCGCTAGGA AAGAGCGCAG GCAGCGCCTC 
ACTGACATCG ATGTGAATAA GTGCAACCGA TTTGGCCACA TCTGCTCCCC ATTCCAAGCC 
TTGATCACCC CTGCCGTACA GGAGATCAGC TTGCCCCCGG GCCTGCCCGT GATGATGAGG 
AGCAGCAGTG GTGCCAGCAG TGAGCGACAG GACTCAGGGA TTGGGGGCAG AATGGATCCA 
GGAGAATTCA ACCCACAAGG GAGTAAAAAC TCACAGACGC TACAAGAGAA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

efcab8 ( XM_031894726.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750586.1 Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa50838
Clone ID Related Accession (Same CDS sequence)	XM_031894727.1 , XM_031894726.1
Accession Version	XM_031894726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	WD repeat-containing protein on Y chromosome isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTTC CGACTCTTCT TCAGAAACGT GCCAATGCCC TACAGAGACA ACTCCTGTCT 
GTCACTCTAC TTCAAGAGCT AGAACACTTC CTAAAGAAGC AGGGGCTTCA AAAATGTTCT 
GGGCAAAAGG TGAAGTTCTG CAATGAGGAA AGCATCTTAA GCTTCGATCG CAATGAAAAC 
CTGCGCTTGG AGGAGAAGTT CCGTTTGGAT GACCTGCGCC TCCTGCAGGA AGCCTTCGCT 
AAGCACAATT CCGACGGCAA AGAGACCAAT TGCAAAGAGG AGTCCGACGA TCTCCGAGCG 
ACGAGGCGCA GAGCGAAACA GGAGGCGCGG CCTGCGGGAA ACATGACCCT CGATGAATTC 
CGCCGGGTTC TCTGCGACCT CTTCGGGTCG GAATGTTTCG ACGACGACAT GGAACTTCTG 
TTTAACGAGG TGGACACCTC CTGTGACGGC TACGTAGACT GGGGGGAGTT CTGCACTTAC 
CTGCTGCTGC AATACAGGAA GCGAGACTAC AGGATGATTG GCAGGGTGAC CTTCCTGGGA 
CAGCCAGTCA TACGGCTTTG CACTCGCAAC AAGCAAGAGC CAACCAGCAG GATCCTCGCC 
ATCAGCTCCC CCCCTCCCAT GTGGCTCCTC AGTGTGAGCA AAACCGGCGT CCTCACTACG 
TGGGATTTGG CCCTGCACCC CCAGAAATCC TACGAGGTTG CTGCTGAATA CCCAGACTCC 
CAAGCCGGGA AGAGGAGGTT CAAGTGCTGG GTAACGGACG CTGTGCATCT GCCCAACGCT 
AATAAAATCG CCATCGCCAC CACCAGCCGT GACATCCACT TCTTTGACGT CTCCACCATG 
AACTTGTTTG AGGAGTTTCA CCTGTTTGCT CTGAATCACG TTCCCACAAC CTTCTCATAT 
TGGTACAACA AACAGTCCCC AGAGAGTCGG TCCCTGCTGT TGTGGGGAGG AGATAATGGG 
GCGATGAATG TTCTTTGGCT GCTCCAACCC CAGACGGGGG TATTTGAGAA ACCATTTAGC 
CAGCATCCGG GCCCTACTAA AATCTACATG CAGGAGCTGA AAGATCACAG CAAACTGCTG 
AGCTACCAGG TTATCCCCGA CGTTCACCCC GAGGCCATCA CCAAACTCAT ATACTGCCCG 
GAGAAGCAAC TGATCATTAC ATCATCGGGA AGCGCCCAAT CCTCGCTGCT CATTATGGAC 
ATTAACCAGA AGGGAAAGGT TTATACGTGG AAGATTAACA AGGGGGTGCA GTGTTTCGAC 
TACTGCCGGG CCTTGAATCT GCTGGTAACT GGGGGGTTCG ACCACAAAGT GCGACTATGG 
AATCAATACG TCACGTGTCG CCCCATTGCC GTCCTACCGG AACATTCCAT GGCCATCCTG 
GATGTGATGA TCTATCAGCC CCTGGGGCAA ATATTCAGCT TCTCCAAAGA CTCTGTGCTA 
AAGGTTTGGG ACATCCCCTC ACAGACGTGC CTGCACACCC TGGTGCTGAA GTTCCCCTGT 
ATCCAGCCCG GCAGGATCCA AGAGCAGGGC AGCTTCCCCT TCCTGCTGGT ACCCACCAAC 
CCCAGCCGCT TACTGGTCTG CTACTCCGAC TATATTGGCA TTCTGAGACT GGCACGGGCT 
GAGCCCACGG AGGGCACTTT CTTCACACAC AGGGCACCTG TTACCTGCCT CCTCTACAGC 
AGCTTCTGCC ATCTGATAGT TACGGGCAGC GAAGATTCCA CGGTTGCCGT GTGGGATGTG 
GAGACCGGCG CCAAATCTCT TCTACTCGGC AACGCGCACG GCCAGGAGGA AGTCACTTGC 
ATGGCGCTCG ACAAAGCGCA GAGGAGGCTC ATCACTGGGG CGAGGAACGG CACCATAAAG 
ATCTGGAACA TTCAGAACGG GCACAACCTG CACCAACTGG AGCCGGTGGA GGATGCGGAG 
ATCACTTGCA TCGTGGCCTC GCAGGAGAAT GTGTTCCTCA CTGTCGGCTG GAACCGGAAA 
ATTGTCACTT ACGACATCCC CAAAACCAAT GAGGTGTATG TGCCGGCCAA TCACTCGTGG 
AAAGGAGGGC AACTTCACCA GGAGGATATC CTGACTGCTG ATTTCTGCCC CTCCCTGGGG 
CTTCTTGCTA CAGCCAGTTT TGATGGAGAG ATCATTCTGT GGAACGCGGA GACTCAGAGA 
CTGTATCTGT ATCTGCGGCA GTGTCCCCAA GCAAGGTCTC CGGCACCGGT GGATAAACTC 
TTCTTCCTCC AGAAACGTTC GGCAAAGAGA GAAGCCGCCA TTCTGGTCAG TTCGGAGGCG 
GGGACCCTGC GTTGGTGGCG AGTGACCAAT GGTGGGCAGG AATGGGGGAG TTTCTATGCC 
CAGGATGAGG CAGACTGCTC TGTATTGGGG TTAGCAAGTG ACAAGGCAAA CAGGGTTCTG 
GTATCGGGAG ACACCCTGGG ATTGGTCCAG GTGTGGGACA TTTCCGCCTA TGGGCTACAG 
GCCGCCGCCC AGACCTACAC CTGTAGGCCA CCCCTACTTT ACTCTTGGGG GGCCCATGAC 
AGCGCCGTAG TCAGTGTGGA ACATTTCTTG TACGACTCGG AACCATTCAT TGCCAGCGGC 
TCCTCCGACA AAACGGCCAA ACTGTGGACA GTGGACGGGA AGCAAGTGGG AACGTTCGGC 
CAATCCAAAT GCTGGAAACT AAGTGACCCC ACCTCTTACC TGGACAGCAG GTCGGAGGGA 
AGTGAAGGAA AGGGCCCCCG GGGCGCAGGA GGGCTGGAGA TCCTGCCGGG AGTAACTGAG 
ATACAGGAGG AGAATAGCGA GGGCAGAGCT CTAACACCCG CCCCCGAGGA TCTGCCTGAG 
ATACTGAGCC AAGAAATTCC TGACCTGGCA ACAGGATCAA GTTCCGAGGC CAGCTTGGGA 
AGTGCGCAGG AAGAGAGCAC AAACATGGCC GCCGCTAGGA AAGAGCGCAG GCAGCGCCTC 
ACTGACATCG ATGTGAATAA GTGCAACCGA TTTGGCCACA TCTGCTCCCC ATTCCAAGCC 
TTGATCACCC CTGCCGTACA GGAGATCAGC TTGCCCCCGG GCCTGCCCGT GATGATGAGG 
AGCAGCAGTG GTGCCAGCAG TGAGCGACAG GACTCAGGGA TTGGGGGCAG AATGGATCCA 
GGAGAATTCA ACCCACAAGG GAGTAAAAAC TCACAGACGC TACAAGAGAA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750586.1
CDS	189..3365
Translation	MPLPTLLQKRANALQRQLLSVTLLQELEHFLKKQGLQKCSGQKVKFCNEESILSFDRNENLRLEEKFRLDDLRLLQEAFAKHNSDGKETNCKEESDDLRATRRRAKQEARPAGNMTLDEFRRVLCDLFGSECFDDDMELLFNEVDTSCDGYVDWGEFCTYLLLQYRKRDYRMIGRVTFLGQPVIRLCTRNKQEPTSRILAISSPPPMWLLSVSKTGVLTTWDLALHPQKSYEVAAEYPDSQAGKRRFKCWVTDAVHLPNANKIAIATTSRDIHFFDVSTMNLFEEFHLFALNHVPTTFSYWYNKQSPESRSLLLWGGDNGAMNVLWLLQPQTGVFEKPFSQHPGPTKIYMQELKDHSKLLSYQVIPDVHPEAITKLIYCPEKQLIITSSGSAQSSLLIMDINQKGKVYTWKINKGVQCFDYCRALNLLVTGGFDHKVRLWNQYVTCRPIAVLPEHSMAILDVMIYQPLGQIFSFSKDSVLKVWDIPSQTCLHTLVLKFPCIQPGRIQEQGSFPFLLVPTNPSRLLVCYSDYIGILRLARAEPTEGTFFTHRAPVTCLLYSSFCHLIVTGSEDSTVAVWDVETGAKSLLLGNAHGQEEVTCMALDKAQRRLITGARNGTIKIWNIQNGHNLHQLEPVEDAEITCIVASQENVFLTVGWNRKIVTYDIPKTNEVYVPANHSWKGGQLHQEDILTADFCPSLGLLATASFDGEIILWNAETQRLYLYLRQCPQARSPAPVDKLFFLQKRSAKREAAILVSSEAGTLRWWRVTNGGQEWGSFYAQDEADCSVLGLASDKANRVLVSGDTLGLVQVWDISAYGLQAAAQTYTCRPPLLYSWGAHDSAVVSVEHFLYDSEPFIASGSSDKTAKLWTVDGKQVGTFGQSKCWKLSDPTSYLDSRSEGSEGKGPRGAGGLEILPGVTEIQEENSEGRALTPAPEDLPEILSQEIPDLATGSSSEASLGSAQEESTNMAAARKERRQRLTDIDVNKCNRFGHICSPFQALITPAVQEISLPPGLPVMMRSSSGASSERQDSGIGGRMDPGEFNPQGSKNSQTLQEKT

Target ORF information:

RefSeq Version	XM_031894726.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894726.1

ORF Insert Sequence:

ATGCCCCTTC CGACTCTTCT TCAGAAACGT GCCAATGCCC TACAGAGACA ACTCCTGTCT 
GTCACTCTAC TTCAAGAGCT AGAACACTTC CTAAAGAAGC AGGGGCTTCA AAAATGTTCT 
GGGCAAAAGG TGAAGTTCTG CAATGAGGAA AGCATCTTAA GCTTCGATCG CAATGAAAAC 
CTGCGCTTGG AGGAGAAGTT CCGTTTGGAT GACCTGCGCC TCCTGCAGGA AGCCTTCGCT 
AAGCACAATT CCGACGGCAA AGAGACCAAT TGCAAAGAGG AGTCCGACGA TCTCCGAGCG 
ACGAGGCGCA GAGCGAAACA GGAGGCGCGG CCTGCGGGAA ACATGACCCT CGATGAATTC 
CGCCGGGTTC TCTGCGACCT CTTCGGGTCG GAATGTTTCG ACGACGACAT GGAACTTCTG 
TTTAACGAGG TGGACACCTC CTGTGACGGC TACGTAGACT GGGGGGAGTT CTGCACTTAC 
CTGCTGCTGC AATACAGGAA GCGAGACTAC AGGATGATTG GCAGGGTGAC CTTCCTGGGA 
CAGCCAGTCA TACGGCTTTG CACTCGCAAC AAGCAAGAGC CAACCAGCAG GATCCTCGCC 
ATCAGCTCCC CCCCTCCCAT GTGGCTCCTC AGTGTGAGCA AAACCGGCGT CCTCACTACG 
TGGGATTTGG CCCTGCACCC CCAGAAATCC TACGAGGTTG CTGCTGAATA CCCAGACTCC 
CAAGCCGGGA AGAGGAGGTT CAAGTGCTGG GTAACGGACG CTGTGCATCT GCCCAACGCT 
AATAAAATCG CCATCGCCAC CACCAGCCGT GACATCCACT TCTTTGACGT CTCCACCATG 
AACTTGTTTG AGGAGTTTCA CCTGTTTGCT CTGAATCACG TTCCCACAAC CTTCTCATAT 
TGGTACAACA AACAGTCCCC AGAGAGTCGG TCCCTGCTGT TGTGGGGAGG AGATAATGGG 
GCGATGAATG TTCTTTGGCT GCTCCAACCC CAGACGGGGG TATTTGAGAA ACCATTTAGC 
CAGCATCCGG GCCCTACTAA AATCTACATG CAGGAGCTGA AAGATCACAG CAAACTGCTG 
AGCTACCAGG TTATCCCCGA CGTTCACCCC GAGGCCATCA CCAAACTCAT ATACTGCCCG 
GAGAAGCAAC TGATCATTAC ATCATCGGGA AGCGCCCAAT CCTCGCTGCT CATTATGGAC 
ATTAACCAGA AGGGAAAGGT TTATACGTGG AAGATTAACA AGGGGGTGCA GTGTTTCGAC 
TACTGCCGGG CCTTGAATCT GCTGGTAACT GGGGGGTTCG ACCACAAAGT GCGACTATGG 
AATCAATACG TCACGTGTCG CCCCATTGCC GTCCTACCGG AACATTCCAT GGCCATCCTG 
GATGTGATGA TCTATCAGCC CCTGGGGCAA ATATTCAGCT TCTCCAAAGA CTCTGTGCTA 
AAGGTTTGGG ACATCCCCTC ACAGACGTGC CTGCACACCC TGGTGCTGAA GTTCCCCTGT 
ATCCAGCCCG GCAGGATCCA AGAGCAGGGC AGCTTCCCCT TCCTGCTGGT ACCCACCAAC 
CCCAGCCGCT TACTGGTCTG CTACTCCGAC TATATTGGCA TTCTGAGACT GGCACGGGCT 
GAGCCCACGG AGGGCACTTT CTTCACACAC AGGGCACCTG TTACCTGCCT CCTCTACAGC 
AGCTTCTGCC ATCTGATAGT TACGGGCAGC GAAGATTCCA CGGTTGCCGT GTGGGATGTG 
GAGACCGGCG CCAAATCTCT TCTACTCGGC AACGCGCACG GCCAGGAGGA AGTCACTTGC 
ATGGCGCTCG ACAAAGCGCA GAGGAGGCTC ATCACTGGGG CGAGGAACGG CACCATAAAG 
ATCTGGAACA TTCAGAACGG GCACAACCTG CACCAACTGG AGCCGGTGGA GGATGCGGAG 
ATCACTTGCA TCGTGGCCTC GCAGGAGAAT GTGTTCCTCA CTGTCGGCTG GAACCGGAAA 
ATTGTCACTT ACGACATCCC CAAAACCAAT GAGGTGTATG TGCCGGCCAA TCACTCGTGG 
AAAGGAGGGC AACTTCACCA GGAGGATATC CTGACTGCTG ATTTCTGCCC CTCCCTGGGG 
CTTCTTGCTA CAGCCAGTTT TGATGGAGAG ATCATTCTGT GGAACGCGGA GACTCAGAGA 
CTGTATCTGT ATCTGCGGCA GTGTCCCCAA GCAAGGTCTC CGGCACCGGT GGATAAACTC 
TTCTTCCTCC AGAAACGTTC GGCAAAGAGA GAAGCCGCCA TTCTGGTCAG TTCGGAGGCG 
GGGACCCTGC GTTGGTGGCG AGTGACCAAT GGTGGGCAGG AATGGGGGAG TTTCTATGCC 
CAGGATGAGG CAGACTGCTC TGTATTGGGG TTAGCAAGTG ACAAGGCAAA CAGGGTTCTG 
GTATCGGGAG ACACCCTGGG ATTGGTCCAG GTGTGGGACA TTTCCGCCTA TGGGCTACAG 
GCCGCCGCCC AGACCTACAC CTGTAGGCCA CCCCTACTTT ACTCTTGGGG GGCCCATGAC 
AGCGCCGTAG TCAGTGTGGA ACATTTCTTG TACGACTCGG AACCATTCAT TGCCAGCGGC 
TCCTCCGACA AAACGGCCAA ACTGTGGACA GTGGACGGGA AGCAAGTGGG AACGTTCGGC 
CAATCCAAAT GCTGGAAACT AAGTGACCCC ACCTCTTACC TGGACAGCAG GTCGGAGGGA 
AGTGAAGGAA AGGGCCCCCG GGGCGCAGGA GGGCTGGAGA TCCTGCCGGG AGTAACTGAG 
ATACAGGAGG AGAATAGCGA GGGCAGAGCT CTAACACCCG CCCCCGAGGA TCTGCCTGAG 
ATACTGAGCC AAGAAATTCC TGACCTGGCA ACAGGATCAA GTTCCGAGGC CAGCTTGGGA 
AGTGCGCAGG AAGAGAGCAC AAACATGGCC GCCGCTAGGA AAGAGCGCAG GCAGCGCCTC 
ACTGACATCG ATGTGAATAA GTGCAACCGA TTTGGCCACA TCTGCTCCCC ATTCCAAGCC 
TTGATCACCC CTGCCGTACA GGAGATCAGC TTGCCCCCGG GCCTGCCCGT GATGATGAGG 
AGCAGCAGTG GTGCCAGCAG TGAGCGACAG GACTCAGGGA TTGGGGGCAG AATGGATCCA 
GGAGAATTCA ACCCACAAGG GAGTAAAAAC TCACAGACGC TACAAGAGAA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

efcab8 ( XM_031894728.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750588.1 Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa50839
Clone ID Related Accession (Same CDS sequence)	XM_031894728.1
Accession Version	XM_031894728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	WD repeat-containing protein on Y chromosome isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGCA CAGTGAGGAG GAGAAGAAGA GCCAAGGCAC AGGGGCTTCA AAAATGTTCT 
GGGCAAAAGG TGAAGTTCTG CAATGAGGAA AGCATCTTAA GCTTCGATCG CAATGAAAAC 
CTGCGCTTGG AGGAGAAGTT CCGTTTGGAT GACCTGCGCC TCCTGCAGGA AGCCTTCGCT 
AAGCACAATT CCGACGGCAA AGAGACCAAT TGCAAAGAGG AGTCCGACGA TCTCCGAGCG 
ACGAGGCGCA GAGCGAAACA GGAGGCGCGG CCTGCGGGAA ACATGACCCT CGATGAATTC 
CGCCGGGTTC TCTGCGACCT CTTCGGGTCG GAATGTTTCG ACGACGACAT GGAACTTCTG 
TTTAACGAGG TGGACACCTC CTGTGACGGC TACGTAGACT GGGGGGAGTT CTGCACTTAC 
CTGCTGCTGC AATACAGGAA GCGAGACTAC AGGATGATTG GCAGGGTGAC CTTCCTGGGA 
CAGCCAGTCA TACGGCTTTG CACTCGCAAC AAGCAAGAGC CAACCAGCAG GATCCTCGCC 
ATCAGCTCCC CCCCTCCCAT GTGGCTCCTC AGTGTGAGCA AAACCGGCGT CCTCACTACG 
TGGGATTTGG CCCTGCACCC CCAGAAATCC TACGAGGTTG CTGCTGAATA CCCAGACTCC 
CAAGCCGGGA AGAGGAGGTT CAAGTGCTGG GTAACGGACG CTGTGCATCT GCCCAACGCT 
AATAAAATCG CCATCGCCAC CACCAGCCGT GACATCCACT TCTTTGACGT CTCCACCATG 
AACTTGTTTG AGGAGTTTCA CCTGTTTGCT CTGAATCACG TTCCCACAAC CTTCTCATAT 
TGGTACAACA AACAGTCCCC AGAGAGTCGG TCCCTGCTGT TGTGGGGAGG AGATAATGGG 
GCGATGAATG TTCTTTGGCT GCTCCAACCC CAGACGGGGG TATTTGAGAA ACCATTTAGC 
CAGCATCCGG GCCCTACTAA AATCTACATG CAGGAGCTGA AAGATCACAG CAAACTGCTG 
AGCTACCAGG TTATCCCCGA CGTTCACCCC GAGGCCATCA CCAAACTCAT ATACTGCCCG 
GAGAAGCAAC TGATCATTAC ATCATCGGGA AGCGCCCAAT CCTCGCTGCT CATTATGGAC 
ATTAACCAGA AGGGAAAGGT TTATACGTGG AAGATTAACA AGGGGGTGCA GTGTTTCGAC 
TACTGCCGGG CCTTGAATCT GCTGGTAACT GGGGGGTTCG ACCACAAAGT GCGACTATGG 
AATCAATACG TCACGTGTCG CCCCATTGCC GTCCTACCGG AACATTCCAT GGCCATCCTG 
GATGTGATGA TCTATCAGCC CCTGGGGCAA ATATTCAGCT TCTCCAAAGA CTCTGTGCTA 
AAGGTTTGGG ACATCCCCTC ACAGACGTGC CTGCACACCC TGGTGCTGAA GTTCCCCTGT 
ATCCAGCCCG GCAGGATCCA AGAGCAGGGC AGCTTCCCCT TCCTGCTGGT ACCCACCAAC 
CCCAGCCGCT TACTGGTCTG CTACTCCGAC TATATTGGCA TTCTGAGACT GGCACGGGCT 
GAGCCCACGG AGGGCACTTT CTTCACACAC AGGGCACCTG TTACCTGCCT CCTCTACAGC 
AGCTTCTGCC ATCTGATAGT TACGGGCAGC GAAGATTCCA CGGTTGCCGT GTGGGATGTG 
GAGACCGGCG CCAAATCTCT TCTACTCGGC AACGCGCACG GCCAGGAGGA AGTCACTTGC 
ATGGCGCTCG ACAAAGCGCA GAGGAGGCTC ATCACTGGGG CGAGGAACGG CACCATAAAG 
ATCTGGAACA TTCAGAACGG GCACAACCTG CACCAACTGG AGCCGGTGGA GGATGCGGAG 
ATCACTTGCA TCGTGGCCTC GCAGGAGAAT GTGTTCCTCA CTGTCGGCTG GAACCGGAAA 
ATTGTCACTT ACGACATCCC CAAAACCAAT GAGGTGTATG TGCCGGCCAA TCACTCGTGG 
AAAGGAGGGC AACTTCACCA GGAGGATATC CTGACTGCTG ATTTCTGCCC CTCCCTGGGG 
CTTCTTGCTA CAGCCAGTTT TGATGGAGAG ATCATTCTGT GGAACGCGGA GACTCAGAGA 
CTGTATCTGT ATCTGCGGCA GTGTCCCCAA GCAAGGTCTC CGGCACCGGT GGATAAACTC 
TTCTTCCTCC AGAAACGTTC GGCAAAGAGA GAAGCCGCCA TTCTGGTCAG TTCGGAGGCG 
GGGACCCTGC GTTGGTGGCG AGTGACCAAT GGTGGGCAGG AATGGGGGAG TTTCTATGCC 
CAGGATGAGG CAGACTGCTC TGTATTGGGG TTAGCAAGTG ACAAGGCAAA CAGGGTTCTG 
GTATCGGGAG ACACCCTGGG ATTGGTCCAG GTGTGGGACA TTTCCGCCTA TGGGCTACAG 
GCCGCCGCCC AGACCTACAC CTGTAGGCCA CCCCTACTTT ACTCTTGGGG GGCCCATGAC 
AGCGCCGTAG TCAGTGTGGA ACATTTCTTG TACGACTCGG AACCATTCAT TGCCAGCGGC 
TCCTCCGACA AAACGGCCAA ACTGTGGACA GTGGACGGGA AGCAAGTGGG AACGTTCGGC 
CAATCCAAAT GCTGGAAACT AAGTGACCCC ACCTCTTACC TGGACAGCAG GTCGGAGGGA 
AGTGAAGGAA AGGGCCCCCG GGGCGCAGGA GGGCTGGAGA TCCTGCCGGG AGTAACTGAG 
ATACAGGAGG AGAATAGCGA GGGCAGAGCT CTAACACCCG CCCCCGAGGA TCTGCCTGAG 
ATACTGAGCC AAGAAATTCC TGACCTGGCA ACAGGATCAA GTTCCGAGGC CAGCTTGGGA 
AGTGCGCAGG AAGAGAGCAC AAACATGGCC GCCGCTAGGA AAGAGCGCAG GCAGCGCCTC 
ACTGACATCG ATGTGAATAA GTGCAACCGA TTTGGCCACA TCTGCTCCCC ATTCCAAGCC 
TTGATCACCC CTGCCGTACA GGAGATCAGC TTGCCCCCGG GCCTGCCCGT GATGATGAGG 
AGCAGCAGTG GTGCCAGCAG TGAGCGACAG GACTCAGGGA TTGGGGGCAG AATGGATCCA 
GGAGAATTCA ACCCACAAGG GAGTAAAAAC TCACAGACGC TACAAGAGAA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750588.1
CDS	121..3237
Translation	MECTVRRRRRAKAQGLQKCSGQKVKFCNEESILSFDRNENLRLEEKFRLDDLRLLQEAFAKHNSDGKETNCKEESDDLRATRRRAKQEARPAGNMTLDEFRRVLCDLFGSECFDDDMELLFNEVDTSCDGYVDWGEFCTYLLLQYRKRDYRMIGRVTFLGQPVIRLCTRNKQEPTSRILAISSPPPMWLLSVSKTGVLTTWDLALHPQKSYEVAAEYPDSQAGKRRFKCWVTDAVHLPNANKIAIATTSRDIHFFDVSTMNLFEEFHLFALNHVPTTFSYWYNKQSPESRSLLLWGGDNGAMNVLWLLQPQTGVFEKPFSQHPGPTKIYMQELKDHSKLLSYQVIPDVHPEAITKLIYCPEKQLIITSSGSAQSSLLIMDINQKGKVYTWKINKGVQCFDYCRALNLLVTGGFDHKVRLWNQYVTCRPIAVLPEHSMAILDVMIYQPLGQIFSFSKDSVLKVWDIPSQTCLHTLVLKFPCIQPGRIQEQGSFPFLLVPTNPSRLLVCYSDYIGILRLARAEPTEGTFFTHRAPVTCLLYSSFCHLIVTGSEDSTVAVWDVETGAKSLLLGNAHGQEEVTCMALDKAQRRLITGARNGTIKIWNIQNGHNLHQLEPVEDAEITCIVASQENVFLTVGWNRKIVTYDIPKTNEVYVPANHSWKGGQLHQEDILTADFCPSLGLLATASFDGEIILWNAETQRLYLYLRQCPQARSPAPVDKLFFLQKRSAKREAAILVSSEAGTLRWWRVTNGGQEWGSFYAQDEADCSVLGLASDKANRVLVSGDTLGLVQVWDISAYGLQAAAQTYTCRPPLLYSWGAHDSAVVSVEHFLYDSEPFIASGSSDKTAKLWTVDGKQVGTFGQSKCWKLSDPTSYLDSRSEGSEGKGPRGAGGLEILPGVTEIQEENSEGRALTPAPEDLPEILSQEIPDLATGSSSEASLGSAQEESTNMAAARKERRQRLTDIDVNKCNRFGHICSPFQALITPAVQEISLPPGLPVMMRSSSGASSERQDSGIGGRMDPGEFNPQGSKNSQTLQEKT

Target ORF information:

RefSeq Version	XM_031894728.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894728.1

ORF Insert Sequence:

ATGGAGTGCA CAGTGAGGAG GAGAAGAAGA GCCAAGGCAC AGGGGCTTCA AAAATGTTCT 
GGGCAAAAGG TGAAGTTCTG CAATGAGGAA AGCATCTTAA GCTTCGATCG CAATGAAAAC 
CTGCGCTTGG AGGAGAAGTT CCGTTTGGAT GACCTGCGCC TCCTGCAGGA AGCCTTCGCT 
AAGCACAATT CCGACGGCAA AGAGACCAAT TGCAAAGAGG AGTCCGACGA TCTCCGAGCG 
ACGAGGCGCA GAGCGAAACA GGAGGCGCGG CCTGCGGGAA ACATGACCCT CGATGAATTC 
CGCCGGGTTC TCTGCGACCT CTTCGGGTCG GAATGTTTCG ACGACGACAT GGAACTTCTG 
TTTAACGAGG TGGACACCTC CTGTGACGGC TACGTAGACT GGGGGGAGTT CTGCACTTAC 
CTGCTGCTGC AATACAGGAA GCGAGACTAC AGGATGATTG GCAGGGTGAC CTTCCTGGGA 
CAGCCAGTCA TACGGCTTTG CACTCGCAAC AAGCAAGAGC CAACCAGCAG GATCCTCGCC 
ATCAGCTCCC CCCCTCCCAT GTGGCTCCTC AGTGTGAGCA AAACCGGCGT CCTCACTACG 
TGGGATTTGG CCCTGCACCC CCAGAAATCC TACGAGGTTG CTGCTGAATA CCCAGACTCC 
CAAGCCGGGA AGAGGAGGTT CAAGTGCTGG GTAACGGACG CTGTGCATCT GCCCAACGCT 
AATAAAATCG CCATCGCCAC CACCAGCCGT GACATCCACT TCTTTGACGT CTCCACCATG 
AACTTGTTTG AGGAGTTTCA CCTGTTTGCT CTGAATCACG TTCCCACAAC CTTCTCATAT 
TGGTACAACA AACAGTCCCC AGAGAGTCGG TCCCTGCTGT TGTGGGGAGG AGATAATGGG 
GCGATGAATG TTCTTTGGCT GCTCCAACCC CAGACGGGGG TATTTGAGAA ACCATTTAGC 
CAGCATCCGG GCCCTACTAA AATCTACATG CAGGAGCTGA AAGATCACAG CAAACTGCTG 
AGCTACCAGG TTATCCCCGA CGTTCACCCC GAGGCCATCA CCAAACTCAT ATACTGCCCG 
GAGAAGCAAC TGATCATTAC ATCATCGGGA AGCGCCCAAT CCTCGCTGCT CATTATGGAC 
ATTAACCAGA AGGGAAAGGT TTATACGTGG AAGATTAACA AGGGGGTGCA GTGTTTCGAC 
TACTGCCGGG CCTTGAATCT GCTGGTAACT GGGGGGTTCG ACCACAAAGT GCGACTATGG 
AATCAATACG TCACGTGTCG CCCCATTGCC GTCCTACCGG AACATTCCAT GGCCATCCTG 
GATGTGATGA TCTATCAGCC CCTGGGGCAA ATATTCAGCT TCTCCAAAGA CTCTGTGCTA 
AAGGTTTGGG ACATCCCCTC ACAGACGTGC CTGCACACCC TGGTGCTGAA GTTCCCCTGT 
ATCCAGCCCG GCAGGATCCA AGAGCAGGGC AGCTTCCCCT TCCTGCTGGT ACCCACCAAC 
CCCAGCCGCT TACTGGTCTG CTACTCCGAC TATATTGGCA TTCTGAGACT GGCACGGGCT 
GAGCCCACGG AGGGCACTTT CTTCACACAC AGGGCACCTG TTACCTGCCT CCTCTACAGC 
AGCTTCTGCC ATCTGATAGT TACGGGCAGC GAAGATTCCA CGGTTGCCGT GTGGGATGTG 
GAGACCGGCG CCAAATCTCT TCTACTCGGC AACGCGCACG GCCAGGAGGA AGTCACTTGC 
ATGGCGCTCG ACAAAGCGCA GAGGAGGCTC ATCACTGGGG CGAGGAACGG CACCATAAAG 
ATCTGGAACA TTCAGAACGG GCACAACCTG CACCAACTGG AGCCGGTGGA GGATGCGGAG 
ATCACTTGCA TCGTGGCCTC GCAGGAGAAT GTGTTCCTCA CTGTCGGCTG GAACCGGAAA 
ATTGTCACTT ACGACATCCC CAAAACCAAT GAGGTGTATG TGCCGGCCAA TCACTCGTGG 
AAAGGAGGGC AACTTCACCA GGAGGATATC CTGACTGCTG ATTTCTGCCC CTCCCTGGGG 
CTTCTTGCTA CAGCCAGTTT TGATGGAGAG ATCATTCTGT GGAACGCGGA GACTCAGAGA 
CTGTATCTGT ATCTGCGGCA GTGTCCCCAA GCAAGGTCTC CGGCACCGGT GGATAAACTC 
TTCTTCCTCC AGAAACGTTC GGCAAAGAGA GAAGCCGCCA TTCTGGTCAG TTCGGAGGCG 
GGGACCCTGC GTTGGTGGCG AGTGACCAAT GGTGGGCAGG AATGGGGGAG TTTCTATGCC 
CAGGATGAGG CAGACTGCTC TGTATTGGGG TTAGCAAGTG ACAAGGCAAA CAGGGTTCTG 
GTATCGGGAG ACACCCTGGG ATTGGTCCAG GTGTGGGACA TTTCCGCCTA TGGGCTACAG 
GCCGCCGCCC AGACCTACAC CTGTAGGCCA CCCCTACTTT ACTCTTGGGG GGCCCATGAC 
AGCGCCGTAG TCAGTGTGGA ACATTTCTTG TACGACTCGG AACCATTCAT TGCCAGCGGC 
TCCTCCGACA AAACGGCCAA ACTGTGGACA GTGGACGGGA AGCAAGTGGG AACGTTCGGC 
CAATCCAAAT GCTGGAAACT AAGTGACCCC ACCTCTTACC TGGACAGCAG GTCGGAGGGA 
AGTGAAGGAA AGGGCCCCCG GGGCGCAGGA GGGCTGGAGA TCCTGCCGGG AGTAACTGAG 
ATACAGGAGG AGAATAGCGA GGGCAGAGCT CTAACACCCG CCCCCGAGGA TCTGCCTGAG 
ATACTGAGCC AAGAAATTCC TGACCTGGCA ACAGGATCAA GTTCCGAGGC CAGCTTGGGA 
AGTGCGCAGG AAGAGAGCAC AAACATGGCC GCCGCTAGGA AAGAGCGCAG GCAGCGCCTC 
ACTGACATCG ATGTGAATAA GTGCAACCGA TTTGGCCACA TCTGCTCCCC ATTCCAAGCC 
TTGATCACCC CTGCCGTACA GGAGATCAGC TTGCCCCCGG GCCTGCCCGT GATGATGAGG 
AGCAGCAGTG GTGCCAGCAG TGAGCGACAG GACTCAGGGA TTGGGGGCAG AATGGATCCA 
GGAGAATTCA ACCCACAAGG GAGTAAAAAC TCACAGACGC TACAAGAGAA AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

efcab8 ( XM_012954182.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809636.2 Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X4, mRNA.

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CloneID	OXa50840
Clone ID Related Accession (Same CDS sequence)	XM_012954182.3
Accession Version	XM_012954182.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	WD repeat-containing protein on Y chromosome isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012954182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTACTA AAAGTAACGG ATTCTGTGTC TATGAAGAAG AAATTCCATC GAAAGTAAAA 
CGCTTCGATC GCAATGAAAA CCTGCGCTTG GAGGAGAAGT TCCGTTTGGA TGACCTGCGC 
CTCCTGCAGG AAGCCTTCGC TAAGCACAAT TCCGACGGCA AAGAGACCAA TTGCAAAGAG 
GAGTCCGACG ATCTCCGAGC GACGAGGCGC AGAGCGAAAC AGGAGGCGCG GCCTGCGGGA 
AACATGACCC TCGATGAATT CCGCCGGGTT CTCTGCGACC TCTTCGGGTC GGAATGTTTC 
GACGACGACA TGGAACTTCT GTTTAACGAG GTGGACACCT CCTGTGACGG CTACGTAGAC 
TGGGGGGAGT TCTGCACTTA CCTGCTGCTG CAATACAGGA AGCGAGACTA CAGGATGATT 
GGCAGGGTGA CCTTCCTGGG ACAGCCAGTC ATACGGCTTT GCACTCGCAA CAAGCAAGAG 
CCAACCAGCA GGATCCTCGC CATCAGCTCC CCCCCTCCCA TGTGGCTCCT CAGTGTGAGC 
AAAACCGGCG TCCTCACTAC GTGGGATTTG GCCCTGCACC CCCAGAAATC CTACGAGGTT 
GCTGCTGAAT ACCCAGACTC CCAAGCCGGG AAGAGGAGGT TCAAGTGCTG GGTAACGGAC 
GCTGTGCATC TGCCCAACGC TAATAAAATC GCCATCGCCA CCACCAGCCG TGACATCCAC 
TTCTTTGACG TCTCCACCAT GAACTTGTTT GAGGAGTTTC ACCTGTTTGC TCTGAATCAC 
GTTCCCACAA CCTTCTCATA TTGGTACAAC AAACAGTCCC CAGAGAGTCG GTCCCTGCTG 
TTGTGGGGAG GAGATAATGG GGCGATGAAT GTTCTTTGGC TGCTCCAACC CCAGACGGGG 
GTATTTGAGA AACCATTTAG CCAGCATCCG GGCCCTACTA AAATCTACAT GCAGGAGCTG 
AAAGATCACA GCAAACTGCT GAGCTACCAG GTTATCCCCG ACGTTCACCC CGAGGCCATC 
ACCAAACTCA TATACTGCCC GGAGAAGCAA CTGATCATTA CATCATCGGG AAGCGCCCAA 
TCCTCGCTGC TCATTATGGA CATTAACCAG AAGGGAAAGG TTTATACGTG GAAGATTAAC 
AAGGGGGTGC AGTGTTTCGA CTACTGCCGG GCCTTGAATC TGCTGGTAAC TGGGGGGTTC 
GACCACAAAG TGCGACTATG GAATCAATAC GTCACGTGTC GCCCCATTGC CGTCCTACCG 
GAACATTCCA TGGCCATCCT GGATGTGATG ATCTATCAGC CCCTGGGGCA AATATTCAGC 
TTCTCCAAAG ACTCTGTGCT AAAGGTTTGG GACATCCCCT CACAGACGTG CCTGCACACC 
CTGGTGCTGA AGTTCCCCTG TATCCAGCCC GGCAGGATCC AAGAGCAGGG CAGCTTCCCC 
TTCCTGCTGG TACCCACCAA CCCCAGCCGC TTACTGGTCT GCTACTCCGA CTATATTGGC 
ATTCTGAGAC TGGCACGGGC TGAGCCCACG GAGGGCACTT TCTTCACACA CAGGGCACCT 
GTTACCTGCC TCCTCTACAG CAGCTTCTGC CATCTGATAG TTACGGGCAG CGAAGATTCC 
ACGGTTGCCG TGTGGGATGT GGAGACCGGC GCCAAATCTC TTCTACTCGG CAACGCGCAC 
GGCCAGGAGG AAGTCACTTG CATGGCGCTC GACAAAGCGC AGAGGAGGCT CATCACTGGG 
GCGAGGAACG GCACCATAAA GATCTGGAAC ATTCAGAACG GGCACAACCT GCACCAACTG 
GAGCCGGTGG AGGATGCGGA GATCACTTGC ATCGTGGCCT CGCAGGAGAA TGTGTTCCTC 
ACTGTCGGCT GGAACCGGAA AATTGTCACT TACGACATCC CCAAAACCAA TGAGGTGTAT 
GTGCCGGCCA ATCACTCGTG GAAAGGAGGG CAACTTCACC AGGAGGATAT CCTGACTGCT 
GATTTCTGCC CCTCCCTGGG GCTTCTTGCT ACAGCCAGTT TTGATGGAGA GATCATTCTG 
TGGAACGCGG AGACTCAGAG ACTGTATCTG TATCTGCGGC AGTGTCCCCA AGCAAGGTCT 
CCGGCACCGG TGGATAAACT CTTCTTCCTC CAGAAACGTT CGGCAAAGAG AGAAGCCGCC 
ATTCTGGTCA GTTCGGAGGC GGGGACCCTG CGTTGGTGGC GAGTGACCAA TGGTGGGCAG 
GAATGGGGGA GTTTCTATGC CCAGGATGAG GCAGACTGCT CTGTATTGGG GTTAGCAAGT 
GACAAGGCAA ACAGGGTTCT GGTATCGGGA GACACCCTGG GATTGGTCCA GGTGTGGGAC 
ATTTCCGCCT ATGGGCTACA GGCCGCCGCC CAGACCTACA CCTGTAGGCC ACCCCTACTT 
TACTCTTGGG GGGCCCATGA CAGCGCCGTA GTCAGTGTGG AACATTTCTT GTACGACTCG 
GAACCATTCA TTGCCAGCGG CTCCTCCGAC AAAACGGCCA AACTGTGGAC AGTGGACGGG 
AAGCAAGTGG GAACGTTCGG CCAATCCAAA TGCTGGAAAC TAAGTGACCC CACCTCTTAC 
CTGGACAGCA GGTCGGAGGG AAGTGAAGGA AAGGGCCCCC GGGGCGCAGG AGGGCTGGAG 
ATCCTGCCGG GAGTAACTGA GATACAGGAG GAGAATAGCG AGGGCAGAGC TCTAACACCC 
GCCCCCGAGG ATCTGCCTGA GATACTGAGC CAAGAAATTC CTGACCTGGC AACAGGATCA 
AGTTCCGAGG CCAGCTTGGG AAGTGCGCAG GAAGAGAGCA CAAACATGGC CGCCGCTAGG 
AAAGAGCGCA GGCAGCGCCT CACTGACATC GATGTGAATA AGTGCAACCG ATTTGGCCAC 
ATCTGCTCCC CATTCCAAGC CTTGATCACC CCTGCCGTAC AGGAGATCAG CTTGCCCCCG 
GGCCTGCCCG TGATGATGAG GAGCAGCAGT GGTGCCAGCA GTGAGCGACA GGACTCAGGG 
ATTGGGGGCA GAATGGATCC AGGAGAATTC AACCCACAAG GGAGTAAAAA CTCACAGACG 
CTACAAGAGA AAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809636.2
CDS	82..3159
Translation	MGTKSNGFCVYEEEIPSKVKRFDRNENLRLEEKFRLDDLRLLQEAFAKHNSDGKETNCKEESDDLRATRRRAKQEARPAGNMTLDEFRRVLCDLFGSECFDDDMELLFNEVDTSCDGYVDWGEFCTYLLLQYRKRDYRMIGRVTFLGQPVIRLCTRNKQEPTSRILAISSPPPMWLLSVSKTGVLTTWDLALHPQKSYEVAAEYPDSQAGKRRFKCWVTDAVHLPNANKIAIATTSRDIHFFDVSTMNLFEEFHLFALNHVPTTFSYWYNKQSPESRSLLLWGGDNGAMNVLWLLQPQTGVFEKPFSQHPGPTKIYMQELKDHSKLLSYQVIPDVHPEAITKLIYCPEKQLIITSSGSAQSSLLIMDINQKGKVYTWKINKGVQCFDYCRALNLLVTGGFDHKVRLWNQYVTCRPIAVLPEHSMAILDVMIYQPLGQIFSFSKDSVLKVWDIPSQTCLHTLVLKFPCIQPGRIQEQGSFPFLLVPTNPSRLLVCYSDYIGILRLARAEPTEGTFFTHRAPVTCLLYSSFCHLIVTGSEDSTVAVWDVETGAKSLLLGNAHGQEEVTCMALDKAQRRLITGARNGTIKIWNIQNGHNLHQLEPVEDAEITCIVASQENVFLTVGWNRKIVTYDIPKTNEVYVPANHSWKGGQLHQEDILTADFCPSLGLLATASFDGEIILWNAETQRLYLYLRQCPQARSPAPVDKLFFLQKRSAKREAAILVSSEAGTLRWWRVTNGGQEWGSFYAQDEADCSVLGLASDKANRVLVSGDTLGLVQVWDISAYGLQAAAQTYTCRPPLLYSWGAHDSAVVSVEHFLYDSEPFIASGSSDKTAKLWTVDGKQVGTFGQSKCWKLSDPTSYLDSRSEGSEGKGPRGAGGLEILPGVTEIQEENSEGRALTPAPEDLPEILSQEIPDLATGSSSEASLGSAQEESTNMAAARKERRQRLTDIDVNKCNRFGHICSPFQALITPAVQEISLPPGLPVMMRSSSGASSERQDSGIGGRMDPGEFNPQGSKNSQTLQEKT

Target ORF information:

RefSeq Version	XM_012954182.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EF-hand calcium binding domain 8 (efcab8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954182.3

ORF Insert Sequence:

ATGGGTACTA AAAGTAACGG ATTCTGTGTC TATGAAGAAG AAATTCCATC GAAAGTAAAA 
CGCTTCGATC GCAATGAAAA CCTGCGCTTG GAGGAGAAGT TCCGTTTGGA TGACCTGCGC 
CTCCTGCAGG AAGCCTTCGC TAAGCACAAT TCCGACGGCA AAGAGACCAA TTGCAAAGAG 
GAGTCCGACG ATCTCCGAGC GACGAGGCGC AGAGCGAAAC AGGAGGCGCG GCCTGCGGGA 
AACATGACCC TCGATGAATT CCGCCGGGTT CTCTGCGACC TCTTCGGGTC GGAATGTTTC 
GACGACGACA TGGAACTTCT GTTTAACGAG GTGGACACCT CCTGTGACGG CTACGTAGAC 
TGGGGGGAGT TCTGCACTTA CCTGCTGCTG CAATACAGGA AGCGAGACTA CAGGATGATT 
GGCAGGGTGA CCTTCCTGGG ACAGCCAGTC ATACGGCTTT GCACTCGCAA CAAGCAAGAG 
CCAACCAGCA GGATCCTCGC CATCAGCTCC CCCCCTCCCA TGTGGCTCCT CAGTGTGAGC 
AAAACCGGCG TCCTCACTAC GTGGGATTTG GCCCTGCACC CCCAGAAATC CTACGAGGTT 
GCTGCTGAAT ACCCAGACTC CCAAGCCGGG AAGAGGAGGT TCAAGTGCTG GGTAACGGAC 
GCTGTGCATC TGCCCAACGC TAATAAAATC GCCATCGCCA CCACCAGCCG TGACATCCAC 
TTCTTTGACG TCTCCACCAT GAACTTGTTT GAGGAGTTTC ACCTGTTTGC TCTGAATCAC 
GTTCCCACAA CCTTCTCATA TTGGTACAAC AAACAGTCCC CAGAGAGTCG GTCCCTGCTG 
TTGTGGGGAG GAGATAATGG GGCGATGAAT GTTCTTTGGC TGCTCCAACC CCAGACGGGG 
GTATTTGAGA AACCATTTAG CCAGCATCCG GGCCCTACTA AAATCTACAT GCAGGAGCTG 
AAAGATCACA GCAAACTGCT GAGCTACCAG GTTATCCCCG ACGTTCACCC CGAGGCCATC 
ACCAAACTCA TATACTGCCC GGAGAAGCAA CTGATCATTA CATCATCGGG AAGCGCCCAA 
TCCTCGCTGC TCATTATGGA CATTAACCAG AAGGGAAAGG TTTATACGTG GAAGATTAAC 
AAGGGGGTGC AGTGTTTCGA CTACTGCCGG GCCTTGAATC TGCTGGTAAC TGGGGGGTTC 
GACCACAAAG TGCGACTATG GAATCAATAC GTCACGTGTC GCCCCATTGC CGTCCTACCG 
GAACATTCCA TGGCCATCCT GGATGTGATG ATCTATCAGC CCCTGGGGCA AATATTCAGC 
TTCTCCAAAG ACTCTGTGCT AAAGGTTTGG GACATCCCCT CACAGACGTG CCTGCACACC 
CTGGTGCTGA AGTTCCCCTG TATCCAGCCC GGCAGGATCC AAGAGCAGGG CAGCTTCCCC 
TTCCTGCTGG TACCCACCAA CCCCAGCCGC TTACTGGTCT GCTACTCCGA CTATATTGGC 
ATTCTGAGAC TGGCACGGGC TGAGCCCACG GAGGGCACTT TCTTCACACA CAGGGCACCT 
GTTACCTGCC TCCTCTACAG CAGCTTCTGC CATCTGATAG TTACGGGCAG CGAAGATTCC 
ACGGTTGCCG TGTGGGATGT GGAGACCGGC GCCAAATCTC TTCTACTCGG CAACGCGCAC 
GGCCAGGAGG AAGTCACTTG CATGGCGCTC GACAAAGCGC AGAGGAGGCT CATCACTGGG 
GCGAGGAACG GCACCATAAA GATCTGGAAC ATTCAGAACG GGCACAACCT GCACCAACTG 
GAGCCGGTGG AGGATGCGGA GATCACTTGC ATCGTGGCCT CGCAGGAGAA TGTGTTCCTC 
ACTGTCGGCT GGAACCGGAA AATTGTCACT TACGACATCC CCAAAACCAA TGAGGTGTAT 
GTGCCGGCCA ATCACTCGTG GAAAGGAGGG CAACTTCACC AGGAGGATAT CCTGACTGCT 
GATTTCTGCC CCTCCCTGGG GCTTCTTGCT ACAGCCAGTT TTGATGGAGA GATCATTCTG 
TGGAACGCGG AGACTCAGAG ACTGTATCTG TATCTGCGGC AGTGTCCCCA AGCAAGGTCT 
CCGGCACCGG TGGATAAACT CTTCTTCCTC CAGAAACGTT CGGCAAAGAG AGAAGCCGCC 
ATTCTGGTCA GTTCGGAGGC GGGGACCCTG CGTTGGTGGC GAGTGACCAA TGGTGGGCAG 
GAATGGGGGA GTTTCTATGC CCAGGATGAG GCAGACTGCT CTGTATTGGG GTTAGCAAGT 
GACAAGGCAA ACAGGGTTCT GGTATCGGGA GACACCCTGG GATTGGTCCA GGTGTGGGAC 
ATTTCCGCCT ATGGGCTACA GGCCGCCGCC CAGACCTACA CCTGTAGGCC ACCCCTACTT 
TACTCTTGGG GGGCCCATGA CAGCGCCGTA GTCAGTGTGG AACATTTCTT GTACGACTCG 
GAACCATTCA TTGCCAGCGG CTCCTCCGAC AAAACGGCCA AACTGTGGAC AGTGGACGGG 
AAGCAAGTGG GAACGTTCGG CCAATCCAAA TGCTGGAAAC TAAGTGACCC CACCTCTTAC 
CTGGACAGCA GGTCGGAGGG AAGTGAAGGA AAGGGCCCCC GGGGCGCAGG AGGGCTGGAG 
ATCCTGCCGG GAGTAACTGA GATACAGGAG GAGAATAGCG AGGGCAGAGC TCTAACACCC 
GCCCCCGAGG ATCTGCCTGA GATACTGAGC CAAGAAATTC CTGACCTGGC AACAGGATCA 
AGTTCCGAGG CCAGCTTGGG AAGTGCGCAG GAAGAGAGCA CAAACATGGC CGCCGCTAGG 
AAAGAGCGCA GGCAGCGCCT CACTGACATC GATGTGAATA AGTGCAACCG ATTTGGCCAC 
ATCTGCTCCC CATTCCAAGC CTTGATCACC CCTGCCGTAC AGGAGATCAG CTTGCCCCCG 
GGCCTGCCCG TGATGATGAG GAGCAGCAGT GGTGCCAGCA GTGAGCGACA GGACTCAGGG 
ATTGGGGGCA GAATGGATCC AGGAGAATTC AACCCACAAG GGAGTAAAAA CTCACAGACG 
CTACAAGAGA AAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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efcab8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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