gli1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following gli1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gli1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa06043 XM_002939425.5
Latest version!
Xenopus tropicalis GLI family zinc finger 1 (gli1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa35856 XM_012957827.2
Latest version!
Xenopus tropicalis GLI family zinc finger 1 (gli1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OXa06043 XM_002939425.4 Xenopus tropicalis GLI family zinc finger 1 (gli1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa06043
    Clone ID Related Accession (Same CDS sequence) XM_002939425.4 , XM_002939425.5
    Accession Version XM_002939425.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product zinc finger protein GLI1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939425.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGTCCC GCCAGTGTCC ACCAGCTGCT GTGTTTAACT CCATGAACCC TCCAGTCAAC 
    AGCTATGTGG AACATTGCTA CCTGAGGTCT CCTAATGTTA TGGCGGAAGG AATGAATGAA
    ATGCCCTATT GCCATCAGAC CAACCTTATG ACCAGTCACC ATGGATTTGG ATTGGCACAA
    GCCAGCGATC ACTTGGCAGG CACAGATGTA TCCCGTTTCT CTACACCTCG AAATACAATG
    AAGCTGAGCA AAAAAAGAGC TATGTCGATC TCCCCGTTGT CCGATGCCAG CATTGATCTA
    CAAACCATGA TCAGGACCTC CCCCAATTCC TTGGTTGCTT TCATTAATTC TCGATGTTCT
    TCCGCTAGTG GATCTTATGG ACATTTGTCT ATAGGAACTA TCAGCCCATC TCTTGGTTAT
    CAAAATTGCC TTAACCATCA GAGGCCACAA GGGGGATCTT ATGGAAACAA CCCACTCATG
    CCTTACAACT CCCATGACCA CATGTCCAGC AGAGGAGTGG GCATGCTCCA GCCTCGCAGC
    TCAATCAAGC ACTGCCAGTT GAAGTCGGAG CCATTGTCGA GCACTGGCTT GGATCCGATT
    GGGAGCAAAA GATTAGAGGA TGGGTCAGAA GGAGATATAT CTAGTCCTGC TTCAGTTGGA
    ACACAGGACC CACTACTTGG ATTACTGGAT GGGCGTGATG ACTTGGAGAA GGATGATGGC
    AAACATGAGC CAGAAACAGT GTATGAAACC AACTGTCACT GGGAAAGCTG TACTAAGGAG
    TTCGACACGC AGGAACATCT TGTCCATCAC ATAAACAATG AGCATATTCA CGGGGAAAAG
    AAGGAGTTTG TGTGTCACTG GCAGGACTGT TCACGAGAAC TCAGACCCTT TAAGGCGCAA
    TATATGCTGG TGGTCCATAT GAGGAGGCAC ACGGGGGAGA AACCCCACAA ATGCACATTT
    GAGGGATGTA ACAAAGCTTA TTCACGACTT GAGAACCTGA AGACTCACCT GAGGTCCCAC
    ACCGGCGAGA AACCTTATGT ATGTGAGCAT GAGGGCTGCA ACAAGGCCTT CTCTAATGCC
    TCCGACAGAG CCAAGCACCA AAATCGAACT CACTCCAATG AGAAACCATA TGTATGCAAG
    ATCCCCGGCT GCACCAAGCG CTATACAGAC CCTAGCTCTC TCAGGAAACA TGTTAAAACC
    GTACATGGGC CGGAGGCACA CATTACCAAG AAGCACCGTG GGGATGGGAT GCCTAGGACT
    CAATCTGGTC ATGAGGGATC TGGGAATCAT ACTGTTAAAG GGGAAAATCA GCTGGATATG
    GAAGCAAGCT CTGCTCGCAA GGAGGATGGA AGACTTGCTG TTCCAGATAT TACTTTGAAG
    TCACAGCCCA GCCCCGGTGG ACAGTCATCT TGTAGCAGTG AAAGGTCCCC TTTGGGAAGC
    ACCAACAATA ATGATAGCGG GGTGGAAATG AATGCCAACA CAGGAGGAAG CTTTGAAGAC
    CTCACCAACC TAGATGATAT TCCCTCTGTA GATTCAATAG GCACTGCAGG TGCTTCAGCC
    CTAAGAAAGC TGGAGAATCT TAGGATTGAC AAACTGAACC AGCTACGTAA AGCCCCATCC
    TCAGGAAAGA TGGTCAAACT GCCATCTATT CACAACAGTG GCCCTCAGGG TGATATGTCA
    GTTGTGTGCG GACCACTGGG TATGTCTCAC AATCAACATG GGATAGAACT GCCATCCAGC
    AGTCATGTTA ATCATCTTAA TGACAGGCGG AACAGCACTA CAAGCACCAT GAGCTCGGCC
    TATACTGTGA GCCGCAGATC CTCAGTTGTG TCTCCATACC TTCCCAACCA AAGAGCAGGT
    GATTCTGGCA ACATGGTGGA TTCATATGAT ATTTCTACAG ACCCTTCTGG TCATTCTAAT
    GAAGCTGTCT GTGCTAGCGG GCTTCCGGGC CTTACTCCTG CACAGCAGTA TAGATTGAAG
    GCAAAATATG CTGCAGCAAC TGGTGGTCCT CCTCCTACTC CACTACCCAA CATGGAGAGG
    ATGAATTCAA ATAATAGGAT GGCATTTGCT TCTAGTGATT ATCGTGGATC ATCCATGTCT
    TCCCTGCTGG CTAATAACAT TCATAGAAGA CATAGTAGCA ATGAGTATCA CAACTATGGA
    ACAGGTATTA TTCACCCAGC TCAAGCTCCA GGTGCTGGAA TAAGAAGAGC TAGTGACCCT
    GCACGAACTG GGGGAGATAT TCAAACAGTG CCAAAAGTGC AACGTTTCAA AAGTATGACA
    AATATGAATG TATCTATGAT GGGGAGACAA GCTAGTGGTA TTCAGCAAGC ATACGGAGGA
    TCTGATGCTA ATTTACAAAG ACATATGTTT TCTCCACGGC CTCCCAGCAT TAGTGAGAAT
    GTTTTTATGG AAACGGCTGG CCCTGATGGA GTATGTCATT CAAAGGACCA CAGCATTATC
    CAGCCTAATG AAATGCAGCA CTATGTGAAC TACCAGGGTC AAGGTTCTCA GCTGGCTGCA
    CCCAGTGACC ATATGAACTT TAATCACCAA ATTCATGGGA TAGATGGTCA AAGCCAAAAT
    GTGTATGGTC ATTCTCAGCG AGCTATGAGC AACATGCATC TAAATTCGGA AAATTACTCA
    AGCCAGAGCA ATGTTCCAAC ACAAACAAAT TTCAACCAGT GCCAAATGAC TGGTCAGAGC
    CAGCATTTTC AGAACACAAG GCAAGCGTAC AGCAGTTCTA ATCTGCCTGT ACAGTGGAAT
    GAAGTTAGTT CAGGGACCAT GGACAACCCT GGTCAGAGTC CAAATCACCA AATAATGCAT
    CAAAATATGC CACCAGGAAA TCATTGCCAG AGTCAATTCT CAAACTCTGT GGTCCCAGAA
    ACAACAAAAC AGGGTTGCCC TGTAAACAGA AGTTCATGCC AGCAGGGAAT GTATATGAAT
    AATCAGAAAT ATAACCATGG GGGTCAGGTG CAGGTTAAGC CTGAACAGCA GTTCCACCAT
    TCAGCTCCCA CTATGATGTC CTGTCAAAAC ATGAAGCATC CTTCTAGACA AGAGCATAAT
    TTTACTCAGT CAAATACAAT GCTTTTGTCA CCTGAATCCA CAAACTGTGA TTTCCAAGGG
    CAACAAGACA ACACTCAGAA CTCCTGCTTC AATGTTGGCC TTAACCCAAA TCTACTAAGC
    CCTCAAGGGC GTAGGTCTCA GACACCCATG ATGCAGGTGA AGGAAATGAT GGTCAGAAAC
    TATGTCCAGT CCCAACAAGC ACTAATGTGG GAGCAACATC CAAAAAGTAT GGCCATGATG
    AACAACAGTG GAGAAGATGT GGATACTGGG CAAAACCAGC ACAAGAATAC CCCAAATGCA
    GCTGTGTATA TGAGTCCTAA GTATATCAAT TATCAGTGCA AGCCATCACC AAACAACTTA
    ATGAGCCCAA GCTCCCAGGA CAGCCAATCA AGTCACACCA AAACAATGGG AAGCCCAAGC
    AGTCAGTGTT ACAACTATGA CATGTTGCCC CATCCTCCCT GTGGCCCCAA ACCTTTAAGC
    AGGCAACACA GTATATCAAG TCAGTCAACA TACATGGGAA GTCCGAATCA GCACAGTCCA
    TCTTACCAGC CATCTGAAAC AAGCCCAAGG AGAATGGCCT GTCTTCCTCC AATTCAGCCT
    CAGTCTGAAG TCAGCAATAA CACCAGCATG ATGTATTACA CAGGTCAAAT GGAAATGCAT
    CATGGTAAAG CAGGGGTCCA TAAACTTGCT ACATCACTAA ATCAAAACCA GACAAGCTGT
    GATGGACAAC AACACGGCCA GTACAGCTCT AATCATAGCT TTCTAAAGAC AGACTCTGTG
    CCATACACAA GTTCATGTCC AGGTGCCAAT ACATTAGACA GTCTTGATCT GGAGAATACA
    CAGATTGATT TTACAGCAAT TATAGATGAT GCAGATAATA CTTTGATGTC TGGCAATATA
    AGCCCGAATG GACTAGCAGG AACTTCTCCG GGTTCCTCAC ACTTGACCAC ACTGAGAAAC
    ACAGGAGCTG TTGTTCCAAA CATGGTGGTG GGTGACTTGA ACTCAATGTT AACTTCTCTG
    ACTGGAGAAA ATAAGTACCT AAACACAATC TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939471.1
    CDS711..4826
    Translation

    Target ORF information:

    RefSeq Version XM_002939425.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GLI family zinc finger 1 (gli1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939425.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGTCCC GCCAGTGTCC ACCAGCTGCT GTGTTTAACT CCATGAACCC TCCAGTCAAC 
    AGCTATGTGG AACATTGCTA CCTGAGGTCT CCTAATGTTA TGGCGGAAGG AATGAATGAA
    ATGCCCTATT GCCATCAGAC CAACCTTATG ACCAGTCACC ATGGATTTGG ATTGGCACAA
    GCCAGCGATC ACTTGGCAGG CACAGATGTA TCCCGTTTCT CTACACCTCG AAATACAATG
    AAGCTGAGCA AAAAAAGAGC TATGTCGATC TCCCCGTTGT CCGATGCCAG CATTGATCTA
    CAAACCATGA TCAGGACCTC CCCCAATTCC TTGGTTGCTT TCATTAATTC TCGATGTTCT
    TCCGCTAGTG GATCTTATGG ACATTTGTCT ATAGGAACTA TCAGCCCATC TCTTGGTTAT
    CAAAATTGCC TTAACCATCA GAGGCCACAA GGGGGATCTT ATGGAAACAA CCCACTCATG
    CCTTACAACT CCCATGACCA CATGTCCAGC AGAGGAGTGG GCATGCTCCA GCCTCGCAGC
    TCAATCAAGC ACTGCCAGTT GAAGTCGGAG CCATTGTCGA GCACTGGCTT GGATCCGATT
    GGGAGCAAAA GATTAGAGGA TGGGTCAGAA GGAGATATAT CTAGTCCTGC TTCAGTTGGA
    ACACAGGACC CACTACTTGG ATTACTGGAT GGGCGTGATG ACTTGGAGAA GGATGATGGC
    AAACATGAGC CAGAAACAGT GTATGAAACC AACTGTCACT GGGAAAGCTG TACTAAGGAG
    TTCGACACGC AGGAACATCT TGTCCATCAC ATAAACAATG AGCATATTCA CGGGGAAAAG
    AAGGAGTTTG TGTGTCACTG GCAGGACTGT TCACGAGAAC TCAGACCCTT TAAGGCGCAA
    TATATGCTGG TGGTCCATAT GAGGAGGCAC ACGGGGGAGA AACCCCACAA ATGCACATTT
    GAGGGATGTA ACAAAGCTTA TTCACGACTT GAGAACCTGA AGACTCACCT GAGGTCCCAC
    ACCGGCGAGA AACCTTATGT ATGTGAGCAT GAGGGCTGCA ACAAGGCCTT CTCTAATGCC
    TCCGACAGAG CCAAGCACCA AAATCGAACT CACTCCAATG AGAAACCATA TGTATGCAAG
    ATCCCCGGCT GCACCAAGCG CTATACAGAC CCTAGCTCTC TCAGGAAACA TGTTAAAACC
    GTACATGGGC CGGAGGCACA CATTACCAAG AAGCACCGTG GGGATGGGAT GCCTAGGACT
    CAATCTGGTC ATGAGGGATC TGGGAATCAT ACTGTTAAAG GGGAAAATCA GCTGGATATG
    GAAGCAAGCT CTGCTCGCAA GGAGGATGGA AGACTTGCTG TTCCAGATAT TACTTTGAAG
    TCACAGCCCA GCCCCGGTGG ACAGTCATCT TGTAGCAGTG AAAGGTCCCC TTTGGGAAGC
    ACCAACAATA ATGATAGCGG GGTGGAAATG AATGCCAACA CAGGAGGAAG CTTTGAAGAC
    CTCACCAACC TAGATGATAT TCCCTCTGTA GATTCAATAG GCACTGCAGG TGCTTCAGCC
    CTAAGAAAGC TGGAGAATCT TAGGATTGAC AAACTGAACC AGCTACGTAA AGCCCCATCC
    TCAGGAAAGA TGGTCAAACT GCCATCTATT CACAACAGTG GCCCTCAGGG TGATATGTCA
    GTTGTGTGCG GACCACTGGG TATGTCTCAC AATCAACATG GGATAGAACT GCCATCCAGC
    AGTCATGTTA ATCATCTTAA TGACAGGCGG AACAGCACTA CAAGCACCAT GAGCTCGGCC
    TATACTGTGA GCCGCAGATC CTCAGTTGTG TCTCCATACC TTCCCAACCA AAGAGCAGGT
    GATTCTGGCA ACATGGTGGA TTCATATGAT ATTTCTACAG ACCCTTCTGG TCATTCTAAT
    GAAGCTGTCT GTGCTAGCGG GCTTCCGGGC CTTACTCCTG CACAGCAGTA TAGATTGAAG
    GCAAAATATG CTGCAGCAAC TGGTGGTCCT CCTCCTACTC CACTACCCAA CATGGAGAGG
    ATGAATTCAA ATAATAGGAT GGCATTTGCT TCTAGTGATT ATCGTGGATC ATCCATGTCT
    TCCCTGCTGG CTAATAACAT TCATAGAAGA CATAGTAGCA ATGAGTATCA CAACTATGGA
    ACAGGTATTA TTCACCCAGC TCAAGCTCCA GGTGCTGGAA TAAGAAGAGC TAGTGACCCT
    GCACGAACTG GGGGAGATAT TCAAACAGTG CCAAAAGTGC AACGTTTCAA AAGTATGACA
    AATATGAATG TATCTATGAT GGGGAGACAA GCTAGTGGTA TTCAGCAAGC ATACGGAGGA
    TCTGATGCTA ATTTACAAAG ACATATGTTT TCTCCACGGC CTCCCAGCAT TAGTGAGAAT
    GTTTTTATGG AAACGGCTGG CCCTGATGGA GTATGTCATT CAAAGGACCA CAGCATTATC
    CAGCCTAATG AAATGCAGCA CTATGTGAAC TACCAGGGTC AAGGTTCTCA GCTGGCTGCA
    CCCAGTGACC ATATGAACTT TAATCACCAA ATTCATGGGA TAGATGGTCA AAGCCAAAAT
    GTGTATGGTC ATTCTCAGCG AGCTATGAGC AACATGCATC TAAATTCGGA AAATTACTCA
    AGCCAGAGCA ATGTTCCAAC ACAAACAAAT TTCAACCAGT GCCAAATGAC TGGTCAGAGC
    CAGCATTTTC AGAACACAAG GCAAGCGTAC AGCAGTTCTA ATCTGCCTGT ACAGTGGAAT
    GAAGTTAGTT CAGGGACCAT GGACAACCCT GGTCAGAGTC CAAATCACCA AATAATGCAT
    CAAAATATGC CACCAGGAAA TCATTGCCAG AGTCAATTCT CAAACTCTGT GGTCCCAGAA
    ACAACAAAAC AGGGTTGCCC TGTAAACAGA AGTTCATGCC AGCAGGGAAT GTATATGAAT
    AATCAGAAAT ATAACCATGG GGGTCAGGTG CAGGTTAAGC CTGAACAGCA GTTCCACCAT
    TCAGCTCCCA CTATGATGTC CTGTCAAAAC ATGAAGCATC CTTCTAGACA AGAGCATAAT
    TTTACTCAGT CAAATACAAT GCTTTTGTCA CCTGAATCCA CAAACTGTGA TTTCCAAGGG
    CAACAAGACA ACACTCAGAA CTCCTGCTTC AATGTTGGCC TTAACCCAAA TCTACTAAGC
    CCTCAAGGGC GTAGGTCTCA GACACCCATG ATGCAGGTGA AGGAAATGAT GGTCAGAAAC
    TATGTCCAGT CCCAACAAGC ACTAATGTGG GAGCAACATC CAAAAAGTAT GGCCATGATG
    AACAACAGTG GAGAAGATGT GGATACTGGG CAAAACCAGC ACAAGAATAC CCCAAATGCA
    GCTGTGTATA TGAGTCCTAA GTATATCAAT TATCAGTGCA AGCCATCACC AAACAACTTA
    ATGAGCCCAA GCTCCCAGGA CAGCCAATCA AGTCACACCA AAACAATGGG AAGCCCAAGC
    AGTCAGTGTT ACAACTATGA CATGTTGCCC CATCCTCCCT GTGGCCCCAA ACCTTTAAGC
    AGGCAACACA GTATATCAAG TCAGTCAACA TACATGGGAA GTCCGAATCA GCACAGTCCA
    TCTTACCAGC CATCTGAAAC AAGCCCAAGG AGAATGGCCT GTCTTCCTCC AATTCAGCCT
    CAGTCTGAAG TCAGCAATAA CACCAGCATG ATGTATTACA CAGGTCAAAT GGAAATGCAT
    CATGGTAAAG CAGGGGTCCA TAAACTTGCT ACATCACTAA ATCAAAACCA GACAAGCTGT
    GATGGACAAC AACACGGCCA GTACAGCTCT AATCATAGCT TTCTAAAGAC AGACTCTGTG
    CCATACACAA GTTCATGTCC AGGTGCCAAT ACATTAGACA GTCTTGATCT GGAGAATACA
    CAGATTGATT TTACAGCAAT TATAGATGAT GCAGATAATA CTTTGATGTC TGGCAATATA
    AGCCCGAATG GACTAGCAGG AACTTCTCCG GGTTCCTCAC ACTTGACCAC ACTGAGAAAC
    ACAGGAGCTG TTGTTCCAAA CATGGTGGTG GGTGACTTGA ACTCAATGTT AACTTCTCTG
    ACTGGAGAAA ATAAGTACCT AAACACAATC TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06043
    Clone ID Related Accession (Same CDS sequence) XM_002939425.4 , XM_002939425.5
    Accession Version XM_002939425.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939425.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGTCCC GCCAGTGTCC ACCAGCTGCT GTGTTTAACT CCATGAACCC TCCAGTCAAC 
    AGCTATGTGG AACATTGCTA CCTGAGGTCT CCTAATGTTA TGGCGGAAGG AATGAATGAA
    ATGCCCTATT GCCATCAGAC CAACCTTATG ACCAGTCACC ATGGATTTGG ATTGGCACAA
    GCCAGCGATC ACTTGGCAGG CACAGATGTA TCCCGTTTCT CTACACCTCG AAATACAATG
    AAGCTGAGCA AAAAAAGAGC TATGTCGATC TCCCCGTTGT CCGATGCCAG CATTGATCTA
    CAAACCATGA TCAGGACCTC CCCCAATTCC TTGGTTGCTT TCATTAATTC TCGATGTTCT
    TCCGCTAGTG GATCTTATGG ACATTTGTCT ATAGGAACTA TCAGCCCATC TCTTGGTTAT
    CAAAATTGCC TTAACCATCA GAGGCCACAA GGGGGATCTT ATGGAAACAA CCCACTCATG
    CCTTACAACT CCCATGACCA CATGTCCAGC AGAGGAGTGG GCATGCTCCA GCCTCGCAGC
    TCAATCAAGC ACTGCCAGTT GAAGTCGGAG CCATTGTCGA GCACTGGCTT GGATCCGATT
    GGGAGCAAAA GATTAGAGGA TGGGTCAGAA GGAGATATAT CTAGTCCTGC TTCAGTTGGA
    ACACAGGACC CACTACTTGG ATTACTGGAT GGGCGTGATG ACTTGGAGAA GGATGATGGC
    AAACATGAGC CAGAAACAGT GTATGAAACC AACTGTCACT GGGAAAGCTG TACTAAGGAG
    TTCGACACGC AGGAACATCT TGTCCATCAC ATAAACAATG AGCATATTCA CGGGGAAAAG
    AAGGAGTTTG TGTGTCACTG GCAGGACTGT TCACGAGAAC TCAGACCCTT TAAGGCGCAA
    TATATGCTGG TGGTCCATAT GAGGAGGCAC ACGGGGGAGA AACCCCACAA ATGCACATTT
    GAGGGATGTA ACAAAGCTTA TTCACGACTT GAGAACCTGA AGACTCACCT GAGGTCCCAC
    ACCGGCGAGA AACCTTATGT ATGTGAGCAT GAGGGCTGCA ACAAGGCCTT CTCTAATGCC
    TCCGACAGAG CCAAGCACCA AAATCGAACT CACTCCAATG AGAAACCATA TGTATGCAAG
    ATCCCCGGCT GCACCAAGCG CTATACAGAC CCTAGCTCTC TCAGGAAACA TGTTAAAACC
    GTACATGGGC CGGAGGCACA CATTACCAAG AAGCACCGTG GGGATGGGAT GCCTAGGACT
    CAATCTGGTC ATGAGGGATC TGGGAATCAT ACTGTTAAAG GGGAAAATCA GCTGGATATG
    GAAGCAAGCT CTGCTCGCAA GGAGGATGGA AGACTTGCTG TTCCAGATAT TACTTTGAAG
    TCACAGCCCA GCCCCGGTGG ACAGTCATCT TGTAGCAGTG AAAGGTCCCC TTTGGGAAGC
    ACCAACAATA ATGATAGCGG GGTGGAAATG AATGCCAACA CAGGAGGAAG CTTTGAAGAC
    CTCACCAACC TAGATGATAT TCCCTCTGTA GATTCAATAG GCACTGCAGG TGCTTCAGCC
    CTAAGAAAGC TGGAGAATCT TAGGATTGAC AAACTGAACC AGCTACGTAA AGCCCCATCC
    TCAGGAAAGA TGGTCAAACT GCCATCTATT CACAACAGTG GCCCTCAGGG TGATATGTCA
    GTTGTGTGCG GACCACTGGG TATGTCTCAC AATCAACATG GGATAGAACT GCCATCCAGC
    AGTCATGTTA ATCATCTTAA TGACAGGCGG AACAGCACTA CAAGCACCAT GAGCTCGGCC
    TATACTGTGA GCCGCAGATC CTCAGTTGTG TCTCCATACC TTCCCAACCA AAGAGCAGGT
    GATTCTGGCA ACATGGTGGA TTCATATGAT ATTTCTACAG ACCCTTCTGG TCATTCTAAT
    GAAGCTGTCT GTGCTAGCGG GCTTCCGGGC CTTACTCCTG CACAGCAGTA TAGATTGAAG
    GCAAAATATG CTGCAGCAAC TGGTGGTCCT CCTCCTACTC CACTACCCAA CATGGAGAGG
    ATGAATTCAA ATAATAGGAT GGCATTTGCT TCTAGTGATT ATCGTGGATC ATCCATGTCT
    TCCCTGCTGG CTAATAACAT TCATAGAAGA CATAGTAGCA ATGAGTATCA CAACTATGGA
    ACAGGTATTA TTCACCCAGC TCAAGCTCCA GGTGCTGGAA TAAGAAGAGC TAGTGACCCT
    GCACGAACTG GGGGAGATAT TCAAACAGTG CCAAAAGTGC AACGTTTCAA AAGTATGACA
    AATATGAATG TATCTATGAT GGGGAGACAA GCTAGTGGTA TTCAGCAAGC ATACGGAGGA
    TCTGATGCTA ATTTACAAAG ACATATGTTT TCTCCACGGC CTCCCAGCAT TAGTGAGAAT
    GTTTTTATGG AAACGGCTGG CCCTGATGGA GTATGTCATT CAAAGGACCA CAGCATTATC
    CAGCCTAATG AAATGCAGCA CTATGTGAAC TACCAGGGTC AAGGTTCTCA GCTGGCTGCA
    CCCAGTGACC ATATGAACTT TAATCACCAA ATTCATGGGA TAGATGGTCA AAGCCAAAAT
    GTGTATGGTC ATTCTCAGCG AGCTATGAGC AACATGCATC TAAATTCGGA AAATTACTCA
    AGCCAGAGCA ATGTTCCAAC ACAAACAAAT TTCAACCAGT GCCAAATGAC TGGTCAGAGC
    CAGCATTTTC AGAACACAAG GCAAGCGTAC AGCAGTTCTA ATCTGCCTGT ACAGTGGAAT
    GAAGTTAGTT CAGGGACCAT GGACAACCCT GGTCAGAGTC CAAATCACCA AATAATGCAT
    CAAAATATGC CACCAGGAAA TCATTGCCAG AGTCAATTCT CAAACTCTGT GGTCCCAGAA
    ACAACAAAAC AGGGTTGCCC TGTAAACAGA AGTTCATGCC AGCAGGGAAT GTATATGAAT
    AATCAGAAAT ATAACCATGG GGGTCAGGTG CAGGTTAAGC CTGAACAGCA GTTCCACCAT
    TCAGCTCCCA CTATGATGTC CTGTCAAAAC ATGAAGCATC CTTCTAGACA AGAGCATAAT
    TTTACTCAGT CAAATACAAT GCTTTTGTCA CCTGAATCCA CAAACTGTGA TTTCCAAGGG
    CAACAAGACA ACACTCAGAA CTCCTGCTTC AATGTTGGCC TTAACCCAAA TCTACTAAGC
    CCTCAAGGGC GTAGGTCTCA GACACCCATG ATGCAGGTGA AGGAAATGAT GGTCAGAAAC
    TATGTCCAGT CCCAACAAGC ACTAATGTGG GAGCAACATC CAAAAAGTAT GGCCATGATG
    AACAACAGTG GAGAAGATGT GGATACTGGG CAAAACCAGC ACAAGAATAC CCCAAATGCA
    GCTGTGTATA TGAGTCCTAA GTATATCAAT TATCAGTGCA AGCCATCACC AAACAACTTA
    ATGAGCCCAA GCTCCCAGGA CAGCCAATCA AGTCACACCA AAACAATGGG AAGCCCAAGC
    AGTCAGTGTT ACAACTATGA CATGTTGCCC CATCCTCCCT GTGGCCCCAA ACCTTTAAGC
    AGGCAACACA GTATATCAAG TCAGTCAACA TACATGGGAA GTCCGAATCA GCACAGTCCA
    TCTTACCAGC CATCTGAAAC AAGCCCAAGG AGAATGGCCT GTCTTCCTCC AATTCAGCCT
    CAGTCTGAAG TCAGCAATAA CACCAGCATG ATGTATTACA CAGGTCAAAT GGAAATGCAT
    CATGGTAAAG CAGGGGTCCA TAAACTTGCT ACATCACTAA ATCAAAACCA GACAAGCTGT
    GATGGACAAC AACACGGCCA GTACAGCTCT AATCATAGCT TTCTAAAGAC AGACTCTGTG
    CCATACACAA GTTCATGTCC AGGTGCCAAT ACATTAGACA GTCTTGATCT GGAGAATACA
    CAGATTGATT TTACAGCAAT TATAGATGAT GCAGATAATA CTTTGATGTC TGGCAATATA
    AGCCCGAATG GACTAGCAGG AACTTCTCCG GGTTCCTCAC ACTTGACCAC ACTGAGAAAC
    ACAGGAGCTG TTGTTCCAAA CATGGTGGTG GGTGACTTGA ACTCAATGTT AACTTCTCTG
    ACTGGAGAAA ATAAGTACCT AAACACAATC TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939471.1
    CDS626..4741
    Translation

    Target ORF information:

    RefSeq Version XM_002939425.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GLI family zinc finger 1 (gli1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939425.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGTCCC GCCAGTGTCC ACCAGCTGCT GTGTTTAACT CCATGAACCC TCCAGTCAAC 
    AGCTATGTGG AACATTGCTA CCTGAGGTCT CCTAATGTTA TGGCGGAAGG AATGAATGAA
    ATGCCCTATT GCCATCAGAC CAACCTTATG ACCAGTCACC ATGGATTTGG ATTGGCACAA
    GCCAGCGATC ACTTGGCAGG CACAGATGTA TCCCGTTTCT CTACACCTCG AAATACAATG
    AAGCTGAGCA AAAAAAGAGC TATGTCGATC TCCCCGTTGT CCGATGCCAG CATTGATCTA
    CAAACCATGA TCAGGACCTC CCCCAATTCC TTGGTTGCTT TCATTAATTC TCGATGTTCT
    TCCGCTAGTG GATCTTATGG ACATTTGTCT ATAGGAACTA TCAGCCCATC TCTTGGTTAT
    CAAAATTGCC TTAACCATCA GAGGCCACAA GGGGGATCTT ATGGAAACAA CCCACTCATG
    CCTTACAACT CCCATGACCA CATGTCCAGC AGAGGAGTGG GCATGCTCCA GCCTCGCAGC
    TCAATCAAGC ACTGCCAGTT GAAGTCGGAG CCATTGTCGA GCACTGGCTT GGATCCGATT
    GGGAGCAAAA GATTAGAGGA TGGGTCAGAA GGAGATATAT CTAGTCCTGC TTCAGTTGGA
    ACACAGGACC CACTACTTGG ATTACTGGAT GGGCGTGATG ACTTGGAGAA GGATGATGGC
    AAACATGAGC CAGAAACAGT GTATGAAACC AACTGTCACT GGGAAAGCTG TACTAAGGAG
    TTCGACACGC AGGAACATCT TGTCCATCAC ATAAACAATG AGCATATTCA CGGGGAAAAG
    AAGGAGTTTG TGTGTCACTG GCAGGACTGT TCACGAGAAC TCAGACCCTT TAAGGCGCAA
    TATATGCTGG TGGTCCATAT GAGGAGGCAC ACGGGGGAGA AACCCCACAA ATGCACATTT
    GAGGGATGTA ACAAAGCTTA TTCACGACTT GAGAACCTGA AGACTCACCT GAGGTCCCAC
    ACCGGCGAGA AACCTTATGT ATGTGAGCAT GAGGGCTGCA ACAAGGCCTT CTCTAATGCC
    TCCGACAGAG CCAAGCACCA AAATCGAACT CACTCCAATG AGAAACCATA TGTATGCAAG
    ATCCCCGGCT GCACCAAGCG CTATACAGAC CCTAGCTCTC TCAGGAAACA TGTTAAAACC
    GTACATGGGC CGGAGGCACA CATTACCAAG AAGCACCGTG GGGATGGGAT GCCTAGGACT
    CAATCTGGTC ATGAGGGATC TGGGAATCAT ACTGTTAAAG GGGAAAATCA GCTGGATATG
    GAAGCAAGCT CTGCTCGCAA GGAGGATGGA AGACTTGCTG TTCCAGATAT TACTTTGAAG
    TCACAGCCCA GCCCCGGTGG ACAGTCATCT TGTAGCAGTG AAAGGTCCCC TTTGGGAAGC
    ACCAACAATA ATGATAGCGG GGTGGAAATG AATGCCAACA CAGGAGGAAG CTTTGAAGAC
    CTCACCAACC TAGATGATAT TCCCTCTGTA GATTCAATAG GCACTGCAGG TGCTTCAGCC
    CTAAGAAAGC TGGAGAATCT TAGGATTGAC AAACTGAACC AGCTACGTAA AGCCCCATCC
    TCAGGAAAGA TGGTCAAACT GCCATCTATT CACAACAGTG GCCCTCAGGG TGATATGTCA
    GTTGTGTGCG GACCACTGGG TATGTCTCAC AATCAACATG GGATAGAACT GCCATCCAGC
    AGTCATGTTA ATCATCTTAA TGACAGGCGG AACAGCACTA CAAGCACCAT GAGCTCGGCC
    TATACTGTGA GCCGCAGATC CTCAGTTGTG TCTCCATACC TTCCCAACCA AAGAGCAGGT
    GATTCTGGCA ACATGGTGGA TTCATATGAT ATTTCTACAG ACCCTTCTGG TCATTCTAAT
    GAAGCTGTCT GTGCTAGCGG GCTTCCGGGC CTTACTCCTG CACAGCAGTA TAGATTGAAG
    GCAAAATATG CTGCAGCAAC TGGTGGTCCT CCTCCTACTC CACTACCCAA CATGGAGAGG
    ATGAATTCAA ATAATAGGAT GGCATTTGCT TCTAGTGATT ATCGTGGATC ATCCATGTCT
    TCCCTGCTGG CTAATAACAT TCATAGAAGA CATAGTAGCA ATGAGTATCA CAACTATGGA
    ACAGGTATTA TTCACCCAGC TCAAGCTCCA GGTGCTGGAA TAAGAAGAGC TAGTGACCCT
    GCACGAACTG GGGGAGATAT TCAAACAGTG CCAAAAGTGC AACGTTTCAA AAGTATGACA
    AATATGAATG TATCTATGAT GGGGAGACAA GCTAGTGGTA TTCAGCAAGC ATACGGAGGA
    TCTGATGCTA ATTTACAAAG ACATATGTTT TCTCCACGGC CTCCCAGCAT TAGTGAGAAT
    GTTTTTATGG AAACGGCTGG CCCTGATGGA GTATGTCATT CAAAGGACCA CAGCATTATC
    CAGCCTAATG AAATGCAGCA CTATGTGAAC TACCAGGGTC AAGGTTCTCA GCTGGCTGCA
    CCCAGTGACC ATATGAACTT TAATCACCAA ATTCATGGGA TAGATGGTCA AAGCCAAAAT
    GTGTATGGTC ATTCTCAGCG AGCTATGAGC AACATGCATC TAAATTCGGA AAATTACTCA
    AGCCAGAGCA ATGTTCCAAC ACAAACAAAT TTCAACCAGT GCCAAATGAC TGGTCAGAGC
    CAGCATTTTC AGAACACAAG GCAAGCGTAC AGCAGTTCTA ATCTGCCTGT ACAGTGGAAT
    GAAGTTAGTT CAGGGACCAT GGACAACCCT GGTCAGAGTC CAAATCACCA AATAATGCAT
    CAAAATATGC CACCAGGAAA TCATTGCCAG AGTCAATTCT CAAACTCTGT GGTCCCAGAA
    ACAACAAAAC AGGGTTGCCC TGTAAACAGA AGTTCATGCC AGCAGGGAAT GTATATGAAT
    AATCAGAAAT ATAACCATGG GGGTCAGGTG CAGGTTAAGC CTGAACAGCA GTTCCACCAT
    TCAGCTCCCA CTATGATGTC CTGTCAAAAC ATGAAGCATC CTTCTAGACA AGAGCATAAT
    TTTACTCAGT CAAATACAAT GCTTTTGTCA CCTGAATCCA CAAACTGTGA TTTCCAAGGG
    CAACAAGACA ACACTCAGAA CTCCTGCTTC AATGTTGGCC TTAACCCAAA TCTACTAAGC
    CCTCAAGGGC GTAGGTCTCA GACACCCATG ATGCAGGTGA AGGAAATGAT GGTCAGAAAC
    TATGTCCAGT CCCAACAAGC ACTAATGTGG GAGCAACATC CAAAAAGTAT GGCCATGATG
    AACAACAGTG GAGAAGATGT GGATACTGGG CAAAACCAGC ACAAGAATAC CCCAAATGCA
    GCTGTGTATA TGAGTCCTAA GTATATCAAT TATCAGTGCA AGCCATCACC AAACAACTTA
    ATGAGCCCAA GCTCCCAGGA CAGCCAATCA AGTCACACCA AAACAATGGG AAGCCCAAGC
    AGTCAGTGTT ACAACTATGA CATGTTGCCC CATCCTCCCT GTGGCCCCAA ACCTTTAAGC
    AGGCAACACA GTATATCAAG TCAGTCAACA TACATGGGAA GTCCGAATCA GCACAGTCCA
    TCTTACCAGC CATCTGAAAC AAGCCCAAGG AGAATGGCCT GTCTTCCTCC AATTCAGCCT
    CAGTCTGAAG TCAGCAATAA CACCAGCATG ATGTATTACA CAGGTCAAAT GGAAATGCAT
    CATGGTAAAG CAGGGGTCCA TAAACTTGCT ACATCACTAA ATCAAAACCA GACAAGCTGT
    GATGGACAAC AACACGGCCA GTACAGCTCT AATCATAGCT TTCTAAAGAC AGACTCTGTG
    CCATACACAA GTTCATGTCC AGGTGCCAAT ACATTAGACA GTCTTGATCT GGAGAATACA
    CAGATTGATT TTACAGCAAT TATAGATGAT GCAGATAATA CTTTGATGTC TGGCAATATA
    AGCCCGAATG GACTAGCAGG AACTTCTCCG GGTTCCTCAC ACTTGACCAC ACTGAGAAAC
    ACAGGAGCTG TTGTTCCAAA CATGGTGGTG GGTGACTTGA ACTCAATGTT AACTTCTCTG
    ACTGGAGAAA ATAAGTACCT AAACACAATC TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35856
    Clone ID Related Accession (Same CDS sequence) XM_012957827.2
    Accession Version XM_012957827.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product zinc finger protein GLI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957827.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGTCCC GCCAGTGTCC ACCAGCTGCT GTGTTTAACT CCATGAACCC TCCAGTCAAC 
    AGCTATGTGG AACATTGCTA CCTGAGGTCT CCTAATGTTA TGGCGGAAGT ATCCCGTTTC
    TCTACACCTC GAAATACAAT GAAGCTGAGC AAAAAAAGAG CTATGTCGAT CTCCCCGTTG
    TCCGATGCCA GCATTGATCT ACAAACCATG ATCAGGACCT CCCCCAATTC CTTGGTTGCT
    TTCATTAATT CTCGATGTTC TTCCGCTAGT GGATCTTATG GACATTTGTC TATAGGAACT
    ATCAGCCCAT CTCTTGGTTA TCAAAATTGC CTTAACCATC AGAGGCCACA AGGGGGATCT
    TATGGAAACA ACCCACTCAT GCCTTACAAC TCCCATGACC ACATGTCCAG CAGAGGAGTG
    GGCATGCTCC AGCCTCGCAG CTCAATCAAG CACTGCCAGT TGAAGTCGGA GCCATTGTCG
    AGCACTGGCT TGGATCCGAT TGGGAGCAAA AGATTAGAGG ATGGGTCAGA AGGAGATATA
    TCTAGTCCTG CTTCAGTTGG AACACAGGAC CCACTACTTG GATTACTGGA TGGGCGTGAT
    GACTTGGAGA AGGATGATGG CAAACATGAG CCAGAAACAG TGTATGAAAC CAACTGTCAC
    TGGGAAAGCT GTACTAAGGA GTTCGACACG CAGGAACATC TTGTCCATCA CATAAACAAT
    GAGCATATTC ACGGGGAAAA GAAGGAGTTT GTGTGTCACT GGCAGGACTG TTCACGAGAA
    CTCAGACCCT TTAAGGCGCA ATATATGCTG GTGGTCCATA TGAGGAGGCA CACGGGGGAG
    AAACCCCACA AATGCACATT TGAGGGATGT AACAAAGCTT ATTCACGACT TGAGAACCTG
    AAGACTCACC TGAGGTCCCA CACCGGCGAG AAACCTTATG TATGTGAGCA TGAGGGCTGC
    AACAAGGCCT TCTCTAATGC CTCCGACAGA GCCAAGCACC AAAATCGAAC TCACTCCAAT
    GAGAAACCAT ATGTATGCAA GATCCCCGGC TGCACCAAGC GCTATACAGA CCCTAGCTCT
    CTCAGGAAAC ATGTTAAAAC CGTACATGGG CCGGAGGCAC ACATTACCAA GAAGCACCGT
    GGGGATGGGA TGCCTAGGAC TCAATCTGGT CATGAGGGAT CTGGGAATCA TACTGTTAAA
    GGGGAAAATC AGCTGGATAT GGAAGCAAGC TCTGCTCGCA AGGAGGATGG AAGACTTGCT
    GTTCCAGATA TTACTTTGAA GTCACAGCCC AGCCCCGGTG GACAGTCATC TTGTAGCAGT
    GAAAGGTCCC CTTTGGGAAG CACCAACAAT AATGATAGCG GGGTGGAAAT GAATGCCAAC
    ACAGGAGGAA GCTTTGAAGA CCTCACCAAC CTAGATGATA TTCCCTCTGT AGATTCAATA
    GGCACTGCAG GTGCTTCAGC CCTAAGAAAG CTGGAGAATC TTAGGATTGA CAAACTGAAC
    CAGCTACGTA AAGCCCCATC CTCAGGAAAG ATGGTCAAAC TGCCATCTAT TCACAACAGT
    GGCCCTCAGG GTGATATGTC AGTTGTGTGC GGACCACTGG GTATGTCTCA CAATCAACAT
    GGGATAGAAC TGCCATCCAG CAGTCATGTT AATCATCTTA ATGACAGGCG GAACAGCACT
    ACAAGCACCA TGAGCTCGGC CTATACTGTG AGCCGCAGAT CCTCAGTTGT GTCTCCATAC
    CTTCCCAACC AAAGAGCAGG TGATTCTGGC AACATGGTGG ATTCATATGA TATTTCTACA
    GACCCTTCTG GTCATTCTAA TGAAGCTGTC TGTGCTAGCG GGCTTCCGGG CCTTACTCCT
    GCACAGCAGT ATAGATTGAA GGCAAAATAT GCTGCAGCAA CTGGTGGTCC TCCTCCTACT
    CCACTACCCA ACATGGAGAG GATGAATTCA AATAATAGGA TGGCATTTGC TTCTAGTGAT
    TATCGTGGAT CATCCATGTC TTCCCTGCTG GCTAATAACA TTCATAGAAG ACATAGTAGC
    AATGAGTATC ACAACTATGG AACAGGTATT ATTCACCCAG CTCAAGCTCC AGGTGCTGGA
    ATAAGAAGAG CTAGTGACCC TGCACGAACT GGGGGAGATA TTCAAACAGT GCCAAAAGTG
    CAACGTTTCA AAAGTATGAC AAATATGAAT GTATCTATGA TGGGGAGACA AGCTAGTGGT
    ATTCAGCAAG CATACGGAGG ATCTGATGCT AATTTACAAA GACATATGTT TTCTCCACGG
    CCTCCCAGCA TTAGTGAGAA TGTTTTTATG GAAACGGCTG GCCCTGATGG AGTATGTCAT
    TCAAAGGACC ACAGCATTAT CCAGCCTAAT GAAATGCAGC ACTATGTGAA CTACCAGGGT
    CAAGGTTCTC AGCTGGCTGC ACCCAGTGAC CATATGAACT TTAATCACCA AATTCATGGG
    ATAGATGGTC AAAGCCAAAA TGTGTATGGT CATTCTCAGC GAGCTATGAG CAACATGCAT
    CTAAATTCGG AAAATTACTC AAGCCAGAGC AATGTTCCAA CACAAACAAA TTTCAACCAG
    TGCCAAATGA CTGGTCAGAG CCAGCATTTT CAGAACACAA GGCAAGCGTA CAGCAGTTCT
    AATCTGCCTG TACAGTGGAA TGAAGTTAGT TCAGGGACCA TGGACAACCC TGGTCAGAGT
    CCAAATCACC AAATAATGCA TCAAAATATG CCACCAGGAA ATCATTGCCA GAGTCAATTC
    TCAAACTCTG TGGTCCCAGA AACAACAAAA CAGGGTTGCC CTGTAAACAG AAGTTCATGC
    CAGCAGGGAA TGTATATGAA TAATCAGAAA TATAACCATG GGGGTCAGGT GCAGGTTAAG
    CCTGAACAGC AGTTCCACCA TTCAGCTCCC ACTATGATGT CCTGTCAAAA CATGAAGCAT
    CCTTCTAGAC AAGAGCATAA TTTTACTCAG TCAAATACAA TGCTTTTGTC ACCTGAATCC
    ACAAACTGTG ATTTCCAAGG GCAACAAGAC AACACTCAGA ACTCCTGCTT CAATGTTGGC
    CTTAACCCAA ATCTACTAAG CCCTCAAGGG CGTAGGTCTC AGACACCCAT GATGCAGGTG
    AAGGAAATGA TGGTCAGAAA CTATGTCCAG TCCCAACAAG CACTAATGTG GGAGCAACAT
    CCAAAAAGTA TGGCCATGAT GAACAACAGT GGAGAAGATG TGGATACTGG GCAAAACCAG
    CACAAGAATA CCCCAAATGC AGCTGTGTAT ATGAGTCCTA AGTATATCAA TTATCAGTGC
    AAGCCATCAC CAAACAACTT AATGAGCCCA AGCTCCCAGG ACAGCCAATC AAGTCACACC
    AAAACAATGG GAAGCCCAAG CAGTCAGTGT TACAACTATG ACATGTTGCC CCATCCTCCC
    TGTGGCCCCA AACCTTTAAG CAGGCAACAC AGTATATCAA GTCAGTCAAC ATACATGGGA
    AGTCCGAATC AGCACAGTCC ATCTTACCAG CCATCTGAAA CAAGCCCAAG GAGAATGGCC
    TGTCTTCCTC CAATTCAGCC TCAGTCTGAA GTCAGCAATA ACACCAGCAT GATGTATTAC
    ACAGGTCAAA TGGAAATGCA TCATGGTAAA GCAGGGGTCC ATAAACTTGC TACATCACTA
    AATCAAAACC AGACAAGCTG TGATGGACAA CAACACGGCC AGTACAGCTC TAATCATAGC
    TTTCTAAAGA CAGACTCTGT GCCATACACA AGTTCATGTC CAGGTGCCAA TACATTAGAC
    AGTCTTGATC TGGAGAATAC ACAGATTGAT TTTACAGCAA TTATAGATGA TGCAGATAAT
    ACTTTGATGT CTGGCAATAT AAGCCCGAAT GGACTAGCAG GAACTTCTCC GGGTTCCTCA
    CACTTGACCA CACTGAGAAA CACAGGAGCT GTTGTTCCAA ACATGGTGGT GGGTGACTTG
    AACTCAATGT TAACTTCTCT GACTGGAGAA AATAAGTACC TAAACACAAT CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813281.1
    CDS627..4643
    Translation

    Target ORF information:

    RefSeq Version XM_012957827.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GLI family zinc finger 1 (gli1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957827.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGTCCC GCCAGTGTCC ACCAGCTGCT GTGTTTAACT CCATGAACCC TCCAGTCAAC 
    AGCTATGTGG AACATTGCTA CCTGAGGTCT CCTAATGTTA TGGCGGAAGT ATCCCGTTTC
    TCTACACCTC GAAATACAAT GAAGCTGAGC AAAAAAAGAG CTATGTCGAT CTCCCCGTTG
    TCCGATGCCA GCATTGATCT ACAAACCATG ATCAGGACCT CCCCCAATTC CTTGGTTGCT
    TTCATTAATT CTCGATGTTC TTCCGCTAGT GGATCTTATG GACATTTGTC TATAGGAACT
    ATCAGCCCAT CTCTTGGTTA TCAAAATTGC CTTAACCATC AGAGGCCACA AGGGGGATCT
    TATGGAAACA ACCCACTCAT GCCTTACAAC TCCCATGACC ACATGTCCAG CAGAGGAGTG
    GGCATGCTCC AGCCTCGCAG CTCAATCAAG CACTGCCAGT TGAAGTCGGA GCCATTGTCG
    AGCACTGGCT TGGATCCGAT TGGGAGCAAA AGATTAGAGG ATGGGTCAGA AGGAGATATA
    TCTAGTCCTG CTTCAGTTGG AACACAGGAC CCACTACTTG GATTACTGGA TGGGCGTGAT
    GACTTGGAGA AGGATGATGG CAAACATGAG CCAGAAACAG TGTATGAAAC CAACTGTCAC
    TGGGAAAGCT GTACTAAGGA GTTCGACACG CAGGAACATC TTGTCCATCA CATAAACAAT
    GAGCATATTC ACGGGGAAAA GAAGGAGTTT GTGTGTCACT GGCAGGACTG TTCACGAGAA
    CTCAGACCCT TTAAGGCGCA ATATATGCTG GTGGTCCATA TGAGGAGGCA CACGGGGGAG
    AAACCCCACA AATGCACATT TGAGGGATGT AACAAAGCTT ATTCACGACT TGAGAACCTG
    AAGACTCACC TGAGGTCCCA CACCGGCGAG AAACCTTATG TATGTGAGCA TGAGGGCTGC
    AACAAGGCCT TCTCTAATGC CTCCGACAGA GCCAAGCACC AAAATCGAAC TCACTCCAAT
    GAGAAACCAT ATGTATGCAA GATCCCCGGC TGCACCAAGC GCTATACAGA CCCTAGCTCT
    CTCAGGAAAC ATGTTAAAAC CGTACATGGG CCGGAGGCAC ACATTACCAA GAAGCACCGT
    GGGGATGGGA TGCCTAGGAC TCAATCTGGT CATGAGGGAT CTGGGAATCA TACTGTTAAA
    GGGGAAAATC AGCTGGATAT GGAAGCAAGC TCTGCTCGCA AGGAGGATGG AAGACTTGCT
    GTTCCAGATA TTACTTTGAA GTCACAGCCC AGCCCCGGTG GACAGTCATC TTGTAGCAGT
    GAAAGGTCCC CTTTGGGAAG CACCAACAAT AATGATAGCG GGGTGGAAAT GAATGCCAAC
    ACAGGAGGAA GCTTTGAAGA CCTCACCAAC CTAGATGATA TTCCCTCTGT AGATTCAATA
    GGCACTGCAG GTGCTTCAGC CCTAAGAAAG CTGGAGAATC TTAGGATTGA CAAACTGAAC
    CAGCTACGTA AAGCCCCATC CTCAGGAAAG ATGGTCAAAC TGCCATCTAT TCACAACAGT
    GGCCCTCAGG GTGATATGTC AGTTGTGTGC GGACCACTGG GTATGTCTCA CAATCAACAT
    GGGATAGAAC TGCCATCCAG CAGTCATGTT AATCATCTTA ATGACAGGCG GAACAGCACT
    ACAAGCACCA TGAGCTCGGC CTATACTGTG AGCCGCAGAT CCTCAGTTGT GTCTCCATAC
    CTTCCCAACC AAAGAGCAGG TGATTCTGGC AACATGGTGG ATTCATATGA TATTTCTACA
    GACCCTTCTG GTCATTCTAA TGAAGCTGTC TGTGCTAGCG GGCTTCCGGG CCTTACTCCT
    GCACAGCAGT ATAGATTGAA GGCAAAATAT GCTGCAGCAA CTGGTGGTCC TCCTCCTACT
    CCACTACCCA ACATGGAGAG GATGAATTCA AATAATAGGA TGGCATTTGC TTCTAGTGAT
    TATCGTGGAT CATCCATGTC TTCCCTGCTG GCTAATAACA TTCATAGAAG ACATAGTAGC
    AATGAGTATC ACAACTATGG AACAGGTATT ATTCACCCAG CTCAAGCTCC AGGTGCTGGA
    ATAAGAAGAG CTAGTGACCC TGCACGAACT GGGGGAGATA TTCAAACAGT GCCAAAAGTG
    CAACGTTTCA AAAGTATGAC AAATATGAAT GTATCTATGA TGGGGAGACA AGCTAGTGGT
    ATTCAGCAAG CATACGGAGG ATCTGATGCT AATTTACAAA GACATATGTT TTCTCCACGG
    CCTCCCAGCA TTAGTGAGAA TGTTTTTATG GAAACGGCTG GCCCTGATGG AGTATGTCAT
    TCAAAGGACC ACAGCATTAT CCAGCCTAAT GAAATGCAGC ACTATGTGAA CTACCAGGGT
    CAAGGTTCTC AGCTGGCTGC ACCCAGTGAC CATATGAACT TTAATCACCA AATTCATGGG
    ATAGATGGTC AAAGCCAAAA TGTGTATGGT CATTCTCAGC GAGCTATGAG CAACATGCAT
    CTAAATTCGG AAAATTACTC AAGCCAGAGC AATGTTCCAA CACAAACAAA TTTCAACCAG
    TGCCAAATGA CTGGTCAGAG CCAGCATTTT CAGAACACAA GGCAAGCGTA CAGCAGTTCT
    AATCTGCCTG TACAGTGGAA TGAAGTTAGT TCAGGGACCA TGGACAACCC TGGTCAGAGT
    CCAAATCACC AAATAATGCA TCAAAATATG CCACCAGGAA ATCATTGCCA GAGTCAATTC
    TCAAACTCTG TGGTCCCAGA AACAACAAAA CAGGGTTGCC CTGTAAACAG AAGTTCATGC
    CAGCAGGGAA TGTATATGAA TAATCAGAAA TATAACCATG GGGGTCAGGT GCAGGTTAAG
    CCTGAACAGC AGTTCCACCA TTCAGCTCCC ACTATGATGT CCTGTCAAAA CATGAAGCAT
    CCTTCTAGAC AAGAGCATAA TTTTACTCAG TCAAATACAA TGCTTTTGTC ACCTGAATCC
    ACAAACTGTG ATTTCCAAGG GCAACAAGAC AACACTCAGA ACTCCTGCTT CAATGTTGGC
    CTTAACCCAA ATCTACTAAG CCCTCAAGGG CGTAGGTCTC AGACACCCAT GATGCAGGTG
    AAGGAAATGA TGGTCAGAAA CTATGTCCAG TCCCAACAAG CACTAATGTG GGAGCAACAT
    CCAAAAAGTA TGGCCATGAT GAACAACAGT GGAGAAGATG TGGATACTGG GCAAAACCAG
    CACAAGAATA CCCCAAATGC AGCTGTGTAT ATGAGTCCTA AGTATATCAA TTATCAGTGC
    AAGCCATCAC CAAACAACTT AATGAGCCCA AGCTCCCAGG ACAGCCAATC AAGTCACACC
    AAAACAATGG GAAGCCCAAG CAGTCAGTGT TACAACTATG ACATGTTGCC CCATCCTCCC
    TGTGGCCCCA AACCTTTAAG CAGGCAACAC AGTATATCAA GTCAGTCAAC ATACATGGGA
    AGTCCGAATC AGCACAGTCC ATCTTACCAG CCATCTGAAA CAAGCCCAAG GAGAATGGCC
    TGTCTTCCTC CAATTCAGCC TCAGTCTGAA GTCAGCAATA ACACCAGCAT GATGTATTAC
    ACAGGTCAAA TGGAAATGCA TCATGGTAAA GCAGGGGTCC ATAAACTTGC TACATCACTA
    AATCAAAACC AGACAAGCTG TGATGGACAA CAACACGGCC AGTACAGCTC TAATCATAGC
    TTTCTAAAGA CAGACTCTGT GCCATACACA AGTTCATGTC CAGGTGCCAA TACATTAGAC
    AGTCTTGATC TGGAGAATAC ACAGATTGAT TTTACAGCAA TTATAGATGA TGCAGATAAT
    ACTTTGATGT CTGGCAATAT AAGCCCGAAT GGACTAGCAG GAACTTCTCC GGGTTCCTCA
    CACTTGACCA CACTGAGAAA CACAGGAGCT GTTGTTCCAA ACATGGTGGT GGGTGACTTG
    AACTCAATGT TAACTTCTCT GACTGGAGAA AATAAGTACC TAAACACAAT CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.