GLI1 cDNA ORF clone, Gallus gallus(chicken)

The following GLI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa149629 XM_025145279.1
Latest version!
Gallus gallus GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa149629 XM_025145280.1
Latest version!
Gallus gallus GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa149630 XM_025145281.1
Latest version!
Gallus gallus GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa23251 NM_001305245.1
Latest version!
Gallus gallus GLI family zinc finger 1 (GLI1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa149629
    Clone ID Related Accession (Same CDS sequence) XM_025145279.1 , XM_025145280.1
    Accession Version XM_025145279.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008465.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCAACC CCGTCACGCC TCAGGCCCCC GGCTACGCCG AGCACTGCTG CCCGCGGCCG 
    CTGCACGGAG CCTCTGCGGG CACACCGGGG CTGCAAGGAC TCGATTTCCC AGTGTGCCAT
    CAGCCCAACC TGGCCAGCAG CCACCATGGC TATGGGCTGG TGCCCGGCAC TGAGCACCCG
    GGTGGTGCTG CTGATGGCTC GCGGTTCTCG ACGCCCCGTG GTGCCGGCAA GCTGGGCAAG
    AAGCGGGCAC TGTCCATCTC ACCCCTGTCA GACTCTAGCG TTGACCTGCA GACGGTGATC
    CGCACCTCGC CCAACTCCCT CGTTGCCTTC ATCAACTCCC GTTGCGCTTC CGCCGGTGGC
    TCCTATGGTC ACCTCTCCAT CAGCACCATC AGTCCATCGT TGGGGTACCA GAACCCTCCG
    GGGCAGCAGA AGGGCCAAGG GCAACTGTTT AGCCACACTC CACCCCTGCC ACCGTGTAGC
    TCCCACGAAA CCCTGTCCTC CCGCCCAGGG CTCCTGCATC CCACCCCGGC TCGTGGGACC
    ATCAAACACT GCCAGCAGCT AAAGTTGGAG AGGAGCCTGA GTAGCCCTCT GACAGCCAAA
    TACCCAGAGG AGAAATCGGA GGGTGACATT TCCAGTCCGG CCTCCACCGG CACACAGGAC
    CCCTTGCTGG GGATGCTCAG CGTTCGGGAT GATCTGGAGA AGGAGGATGG GAAACCTGAA
    TCGGAGACCA TCTATGAAAC AAACTGCTAC TGGGATGGCT GCGCCAAGGA GTTTGACACG
    CAGGAGCAGC TGGTGCATCA CATCAACAAC GAGCACATCC ACGGGGAGAA GAAGGAGTTT
    GTGTGCCACT GGGCAGCGTG CTCCCGCGAG CAGCGGCCCT TCAAGGCTCA GTACATGCTG
    GTGGTGCACA TGAGGCGCCA CACGGGCGAG AAGCCTCACA AGTGCACGTT TGAGGGCTGT
    AACAAAGCGT ACTCCCGCCT GGAGAACCTC AAGACCCATC TGCGCTCCCA CACGGGTGAG
    AAACCCTATG TGTGTGAGCA CGAGGGTTGT AACAAAGCCT TCTCCAACGC TTCCGACCGG
    GCCAAGCATC AGAACCGCAC CCACTCCAAT GAGAAGCCAT ACATCTGTAA GATCCCGGGC
    TGCACCAAGC GCTACACGGA TCCCAGCTCC CTGCGCAAAC ACGTGAAGAC GGTGCACGGC
    CCCGACGCCC ACGTCACCAA GAAGCACCGG GGCAGCGTTG TGCCTGGCCA TGCACTGCCT
    GCCTCTGCTG CACCCCAGGA CATGAAGCAG GAGAAAAACA CCAACGGCCC TGCTGAGATC
    CGCAAGGATG ACGGCAAGCT GCTGGTGCCT GACTTGGTGT CGAAGCCACA GCCCAGCCCT
    GGTGGGCAGT CATCGTGCAG CAGTGACCGC TCCCCCCTCG GCAGCACCAC CAACAACGAC
    AGCGGTGTGG AGATGACGGG CAACACGGGT GGCAGCTACG AGGATCTGTC CACGCTGGAG
    GATGTGGTGC CCGGGGATCC CATGGGCACC TCGGGGCTGA TGGCCCTCCA GAAGCTCGAG
    AACCTTCGCA TTGACAAACT GAAGCAGATG AGGAAGCCAT CAACCAAGGG CCTGAACCTG
    CCAGCCATCC CCGGAGCCGG TCTGCCCGGA GATGTGCCCA GGGTCTGCGT GCCGCCGCCG
    GCTGCGTCCC AGCGCCGCGT CGTGGAGCTG TCGGCTGCAG AGGTGGCCGT TCCGCCGAGC
    GAGCGCCGCA GCAGCGCCAC CAGCACCATC AGCTCAGCCT ACACCGTCAG CCGCCGCTCC
    TCCGCCGCCT CCCCGTACCT ACCGGGGCCG AACTTGCCCG GCGAGGGGGG GACGATGCCG
    GCACGCTCGG ATGGCTACGA CTGCATCTCT CCGGATGAGC TGCGGCGCTC CGGCAACGCC
    GATCCCTGCG GGGCGCTGCC GGGCGTGGGG AGCCTCACGG CGGCTCAGCA TTACCGTCTC
    AAAGCCAAAT ACGCCGCGGC CACGGGGGGG CCGCCCCCAA CTCCGCTGCC CGGCGTGGAG
    CCGCCGAACG TGGGCGGCCA CGGCGGTTTG CCCGGGGAGT GCCTCGAGCC GGCCGTGCCC
    TCCTTCTTCG CGAACGGTTT GCTGCGAAGG CACAGCTCCA ACGACCGCTA CGTGAGCAGC
    CTTCACCCTC ACCTGGCGCC TGGCAACGAC GTGAGGAGGG CCAGCGACCC GGCCCGGATT
    GCAGCCAACC CTCACTCTGT GCCCAAAGTG CAGCGTTTTA AAAGCGTGGG GAACGTGAAT
    GCGCCGGGAG GCGGCAGAGC TGCCCTGCAG CCCCTGGGTG CTTCCGACGG CAACCTGCAG
    CGCCGCGTCT TCTCCCCTCA CCCACCCAGC ATCAGTGAGA ATGTTTACCT GGAGAGCGTG
    AACCCGGACG GCCCCGGGCC TGGCGCGGGG TCTGGCTTGC TGGAAATGGA CCCGTTCTTG
    AACTACCCCG AGGAAAGCTT CCCGTGCCAG GGGGCAAACG TGCAGCTGCA GTGCGGGGGT
    CTGTATGGCA CCGCTCAGCG GACCCCGGGG GGCGGAATGC AGCTGAACCC GGGTGGGCAC
    AGGGAGATGG AGGAGGGATT GCTGCAGCCT GAGTTCTCCC TCCCGCAGTG CCAGATGAAC
    CAGCACTTCC ACGGCCTGCG TGGCGGCAGC GGGACCGTGC TGCCTCAATG GGAGGAGCCC
    CCCCAAAGCG ATCACCTGGA GATGGGCTCC GGGCAGCCAG CAGTGAGCGC CGTGCCTGAG
    ACGCACCGCC ACCGTCACAG CGCTCTGTAC CCACCGCCCA ACCACTGCGT GCAGCACCCC
    AAACCGATGA GCACGGGCCA CGATGGTGGC TTTTCAGATG GCCGTCGCTT GAACTGCCTG
    CAGATCAAAT CTGAGCAGCG GTACTCGGCT CCTGCCCCGG CCCTCACCCC GTGCCACGAT
    GCCAAAGCCA CGCGGCCGCC CGCGTTCGGC CGGGCTGTGA GTGGGTACCA GGCTGAGGAG
    CAGCCAGCCG GCGGCTACGC GGGCGTGCTG AGCCCACGGG GGAGGAGAGC CCACACCCCC
    ACTTCGCAGG CCAAAGAGGT GATGGTCCGC AGCTACGTGC AGGCGCAGCA GGCCCTGATG
    TGGGGAGAGC CCCCCAAGGG CGGTGAGGCC GGCGTGGGGC TGGGCGGTGA GTCTGGGCAG
    TACCAGGGCA CGCACGCAGC GCCGTACCTT GGCCCCAAGT ACTCTGGTTA CCAGCCCAAA
    GCCAACCACC TGCAGGGGCT GCTGGAGAAC CCCCACCTCC TGAGCCCCCC GTGCTTTAAC
    CCTGAGATGG TGCCACATCC CCCCAGCGGT GCCAAGCCAC CGGGCCACCA GAACAGCGTG
    GGCTATGGGG GCGACCTGGC AGATCCCATC CCCTCCTACG AGGCAGCGGG TGCCAACCCC
    CAGCGCGGGC TGCGCCTGCC CCCGGTCCAC CCTGCCCCCG AAGTGCCCGG TGATACACTG
    CTCTGCTACC CGGGCCAGGT GGGCAAAGGC AGCCACAAGC TTCAGGGTCC GCCTGCCGCA
    AGCTGTGGGG GTCCCGGGCA CTGCACTGGG AGCCTGGGGG GCAGCAAAGG CAGCTCTTGT
    TTCTACCCAG ACTCGGGGCA GCAGGCATTC AACAGCCTGG ACTCGCTGGA CCTGGAGAAC
    ACTCACCTGG ACTTTGCTGC CATCGTGGAG GATGCAGAGA GCCCCACGCT CCTGCCCGGC
    CCCCCCAGCC CCACTGGGGG TCTCCTGCTG CCAGCGCCCG GCGGTGCCAA CATGGCCGTG
    GGTGACATGA GCTCCATGCT GAGCACTTTG GCTGGGGAGA GCCACTTCCT CAACTCCCTC
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001047.1
    CDS663..4508
    Translation

    Target ORF information:

    RefSeq Version XM_025145279.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145279.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCAACC CCGTCACGCC TCAGGCCCCC GGCTACGCCG AGCACTGCTG CCCGCGGCCG 
    CTGCACGGAG CCTCTGCGGG CACACCGGGG CTGCAAGGAC TCGATTTCCC AGTGTGCCAT
    CAGCCCAACC TGGCCAGCAG CCACCATGGC TATGGGCTGG TGCCCGGCAC TGAGCACCCG
    GGTGGTGCTG CTGATGGCTC GCGGTTCTCG ACGCCCCGTG GTGCCGGCAA GCTGGGCAAG
    AAGCGGGCAC TGTCCATCTC ACCCCTGTCA GACTCTAGCG TTGACCTGCA GACGGTGATC
    CGCACCTCGC CCAACTCCCT CGTTGCCTTC ATCAACTCCC GTTGCGCTTC CGCCGGTGGC
    TCCTATGGTC ACCTCTCCAT CAGCACCATC AGTCCATCGT TGGGGTACCA GAACCCTCCG
    GGGCAGCAGA AGGGCCAAGG GCAACTGTTT AGCCACACTC CACCCCTGCC ACCGTGTAGC
    TCCCACGAAA CCCTGTCCTC CCGCCCAGGG CTCCTGCATC CCACCCCGGC TCGTGGGACC
    ATCAAACACT GCCAGCAGCT AAAGTTGGAG AGGAGCCTGA GTAGCCCTCT GACAGCCAAA
    TACCCAGAGG AGAAATCGGA GGGTGACATT TCCAGTCCGG CCTCCACCGG CACACAGGAC
    CCCTTGCTGG GGATGCTCAG CGTTCGGGAT GATCTGGAGA AGGAGGATGG GAAACCTGAA
    TCGGAGACCA TCTATGAAAC AAACTGCTAC TGGGATGGCT GCGCCAAGGA GTTTGACACG
    CAGGAGCAGC TGGTGCATCA CATCAACAAC GAGCACATCC ACGGGGAGAA GAAGGAGTTT
    GTGTGCCACT GGGCAGCGTG CTCCCGCGAG CAGCGGCCCT TCAAGGCTCA GTACATGCTG
    GTGGTGCACA TGAGGCGCCA CACGGGCGAG AAGCCTCACA AGTGCACGTT TGAGGGCTGT
    AACAAAGCGT ACTCCCGCCT GGAGAACCTC AAGACCCATC TGCGCTCCCA CACGGGTGAG
    AAACCCTATG TGTGTGAGCA CGAGGGTTGT AACAAAGCCT TCTCCAACGC TTCCGACCGG
    GCCAAGCATC AGAACCGCAC CCACTCCAAT GAGAAGCCAT ACATCTGTAA GATCCCGGGC
    TGCACCAAGC GCTACACGGA TCCCAGCTCC CTGCGCAAAC ACGTGAAGAC GGTGCACGGC
    CCCGACGCCC ACGTCACCAA GAAGCACCGG GGCAGCGTTG TGCCTGGCCA TGCACTGCCT
    GCCTCTGCTG CACCCCAGGA CATGAAGCAG GAGAAAAACA CCAACGGCCC TGCTGAGATC
    CGCAAGGATG ACGGCAAGCT GCTGGTGCCT GACTTGGTGT CGAAGCCACA GCCCAGCCCT
    GGTGGGCAGT CATCGTGCAG CAGTGACCGC TCCCCCCTCG GCAGCACCAC CAACAACGAC
    AGCGGTGTGG AGATGACGGG CAACACGGGT GGCAGCTACG AGGATCTGTC CACGCTGGAG
    GATGTGGTGC CCGGGGATCC CATGGGCACC TCGGGGCTGA TGGCCCTCCA GAAGCTCGAG
    AACCTTCGCA TTGACAAACT GAAGCAGATG AGGAAGCCAT CAACCAAGGG CCTGAACCTG
    CCAGCCATCC CCGGAGCCGG TCTGCCCGGA GATGTGCCCA GGGTCTGCGT GCCGCCGCCG
    GCTGCGTCCC AGCGCCGCGT CGTGGAGCTG TCGGCTGCAG AGGTGGCCGT TCCGCCGAGC
    GAGCGCCGCA GCAGCGCCAC CAGCACCATC AGCTCAGCCT ACACCGTCAG CCGCCGCTCC
    TCCGCCGCCT CCCCGTACCT ACCGGGGCCG AACTTGCCCG GCGAGGGGGG GACGATGCCG
    GCACGCTCGG ATGGCTACGA CTGCATCTCT CCGGATGAGC TGCGGCGCTC CGGCAACGCC
    GATCCCTGCG GGGCGCTGCC GGGCGTGGGG AGCCTCACGG CGGCTCAGCA TTACCGTCTC
    AAAGCCAAAT ACGCCGCGGC CACGGGGGGG CCGCCCCCAA CTCCGCTGCC CGGCGTGGAG
    CCGCCGAACG TGGGCGGCCA CGGCGGTTTG CCCGGGGAGT GCCTCGAGCC GGCCGTGCCC
    TCCTTCTTCG CGAACGGTTT GCTGCGAAGG CACAGCTCCA ACGACCGCTA CGTGAGCAGC
    CTTCACCCTC ACCTGGCGCC TGGCAACGAC GTGAGGAGGG CCAGCGACCC GGCCCGGATT
    GCAGCCAACC CTCACTCTGT GCCCAAAGTG CAGCGTTTTA AAAGCGTGGG GAACGTGAAT
    GCGCCGGGAG GCGGCAGAGC TGCCCTGCAG CCCCTGGGTG CTTCCGACGG CAACCTGCAG
    CGCCGCGTCT TCTCCCCTCA CCCACCCAGC ATCAGTGAGA ATGTTTACCT GGAGAGCGTG
    AACCCGGACG GCCCCGGGCC TGGCGCGGGG TCTGGCTTGC TGGAAATGGA CCCGTTCTTG
    AACTACCCCG AGGAAAGCTT CCCGTGCCAG GGGGCAAACG TGCAGCTGCA GTGCGGGGGT
    CTGTATGGCA CCGCTCAGCG GACCCCGGGG GGCGGAATGC AGCTGAACCC GGGTGGGCAC
    AGGGAGATGG AGGAGGGATT GCTGCAGCCT GAGTTCTCCC TCCCGCAGTG CCAGATGAAC
    CAGCACTTCC ACGGCCTGCG TGGCGGCAGC GGGACCGTGC TGCCTCAATG GGAGGAGCCC
    CCCCAAAGCG ATCACCTGGA GATGGGCTCC GGGCAGCCAG CAGTGAGCGC CGTGCCTGAG
    ACGCACCGCC ACCGTCACAG CGCTCTGTAC CCACCGCCCA ACCACTGCGT GCAGCACCCC
    AAACCGATGA GCACGGGCCA CGATGGTGGC TTTTCAGATG GCCGTCGCTT GAACTGCCTG
    CAGATCAAAT CTGAGCAGCG GTACTCGGCT CCTGCCCCGG CCCTCACCCC GTGCCACGAT
    GCCAAAGCCA CGCGGCCGCC CGCGTTCGGC CGGGCTGTGA GTGGGTACCA GGCTGAGGAG
    CAGCCAGCCG GCGGCTACGC GGGCGTGCTG AGCCCACGGG GGAGGAGAGC CCACACCCCC
    ACTTCGCAGG CCAAAGAGGT GATGGTCCGC AGCTACGTGC AGGCGCAGCA GGCCCTGATG
    TGGGGAGAGC CCCCCAAGGG CGGTGAGGCC GGCGTGGGGC TGGGCGGTGA GTCTGGGCAG
    TACCAGGGCA CGCACGCAGC GCCGTACCTT GGCCCCAAGT ACTCTGGTTA CCAGCCCAAA
    GCCAACCACC TGCAGGGGCT GCTGGAGAAC CCCCACCTCC TGAGCCCCCC GTGCTTTAAC
    CCTGAGATGG TGCCACATCC CCCCAGCGGT GCCAAGCCAC CGGGCCACCA GAACAGCGTG
    GGCTATGGGG GCGACCTGGC AGATCCCATC CCCTCCTACG AGGCAGCGGG TGCCAACCCC
    CAGCGCGGGC TGCGCCTGCC CCCGGTCCAC CCTGCCCCCG AAGTGCCCGG TGATACACTG
    CTCTGCTACC CGGGCCAGGT GGGCAAAGGC AGCCACAAGC TTCAGGGTCC GCCTGCCGCA
    AGCTGTGGGG GTCCCGGGCA CTGCACTGGG AGCCTGGGGG GCAGCAAAGG CAGCTCTTGT
    TTCTACCCAG ACTCGGGGCA GCAGGCATTC AACAGCCTGG ACTCGCTGGA CCTGGAGAAC
    ACTCACCTGG ACTTTGCTGC CATCGTGGAG GATGCAGAGA GCCCCACGCT CCTGCCCGGC
    CCCCCCAGCC CCACTGGGGG TCTCCTGCTG CCAGCGCCCG GCGGTGCCAA CATGGCCGTG
    GGTGACATGA GCTCCATGCT GAGCACTTTG GCTGGGGAGA GCCACTTCCT CAACTCCCTC
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149629
    Clone ID Related Accession (Same CDS sequence) XM_025145279.1 , XM_025145280.1
    Accession Version XM_025145280.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008465.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCAACC CCGTCACGCC TCAGGCCCCC GGCTACGCCG AGCACTGCTG CCCGCGGCCG 
    CTGCACGGAG CCTCTGCGGG CACACCGGGG CTGCAAGGAC TCGATTTCCC AGTGTGCCAT
    CAGCCCAACC TGGCCAGCAG CCACCATGGC TATGGGCTGG TGCCCGGCAC TGAGCACCCG
    GGTGGTGCTG CTGATGGCTC GCGGTTCTCG ACGCCCCGTG GTGCCGGCAA GCTGGGCAAG
    AAGCGGGCAC TGTCCATCTC ACCCCTGTCA GACTCTAGCG TTGACCTGCA GACGGTGATC
    CGCACCTCGC CCAACTCCCT CGTTGCCTTC ATCAACTCCC GTTGCGCTTC CGCCGGTGGC
    TCCTATGGTC ACCTCTCCAT CAGCACCATC AGTCCATCGT TGGGGTACCA GAACCCTCCG
    GGGCAGCAGA AGGGCCAAGG GCAACTGTTT AGCCACACTC CACCCCTGCC ACCGTGTAGC
    TCCCACGAAA CCCTGTCCTC CCGCCCAGGG CTCCTGCATC CCACCCCGGC TCGTGGGACC
    ATCAAACACT GCCAGCAGCT AAAGTTGGAG AGGAGCCTGA GTAGCCCTCT GACAGCCAAA
    TACCCAGAGG AGAAATCGGA GGGTGACATT TCCAGTCCGG CCTCCACCGG CACACAGGAC
    CCCTTGCTGG GGATGCTCAG CGTTCGGGAT GATCTGGAGA AGGAGGATGG GAAACCTGAA
    TCGGAGACCA TCTATGAAAC AAACTGCTAC TGGGATGGCT GCGCCAAGGA GTTTGACACG
    CAGGAGCAGC TGGTGCATCA CATCAACAAC GAGCACATCC ACGGGGAGAA GAAGGAGTTT
    GTGTGCCACT GGGCAGCGTG CTCCCGCGAG CAGCGGCCCT TCAAGGCTCA GTACATGCTG
    GTGGTGCACA TGAGGCGCCA CACGGGCGAG AAGCCTCACA AGTGCACGTT TGAGGGCTGT
    AACAAAGCGT ACTCCCGCCT GGAGAACCTC AAGACCCATC TGCGCTCCCA CACGGGTGAG
    AAACCCTATG TGTGTGAGCA CGAGGGTTGT AACAAAGCCT TCTCCAACGC TTCCGACCGG
    GCCAAGCATC AGAACCGCAC CCACTCCAAT GAGAAGCCAT ACATCTGTAA GATCCCGGGC
    TGCACCAAGC GCTACACGGA TCCCAGCTCC CTGCGCAAAC ACGTGAAGAC GGTGCACGGC
    CCCGACGCCC ACGTCACCAA GAAGCACCGG GGCAGCGTTG TGCCTGGCCA TGCACTGCCT
    GCCTCTGCTG CACCCCAGGA CATGAAGCAG GAGAAAAACA CCAACGGCCC TGCTGAGATC
    CGCAAGGATG ACGGCAAGCT GCTGGTGCCT GACTTGGTGT CGAAGCCACA GCCCAGCCCT
    GGTGGGCAGT CATCGTGCAG CAGTGACCGC TCCCCCCTCG GCAGCACCAC CAACAACGAC
    AGCGGTGTGG AGATGACGGG CAACACGGGT GGCAGCTACG AGGATCTGTC CACGCTGGAG
    GATGTGGTGC CCGGGGATCC CATGGGCACC TCGGGGCTGA TGGCCCTCCA GAAGCTCGAG
    AACCTTCGCA TTGACAAACT GAAGCAGATG AGGAAGCCAT CAACCAAGGG CCTGAACCTG
    CCAGCCATCC CCGGAGCCGG TCTGCCCGGA GATGTGCCCA GGGTCTGCGT GCCGCCGCCG
    GCTGCGTCCC AGCGCCGCGT CGTGGAGCTG TCGGCTGCAG AGGTGGCCGT TCCGCCGAGC
    GAGCGCCGCA GCAGCGCCAC CAGCACCATC AGCTCAGCCT ACACCGTCAG CCGCCGCTCC
    TCCGCCGCCT CCCCGTACCT ACCGGGGCCG AACTTGCCCG GCGAGGGGGG GACGATGCCG
    GCACGCTCGG ATGGCTACGA CTGCATCTCT CCGGATGAGC TGCGGCGCTC CGGCAACGCC
    GATCCCTGCG GGGCGCTGCC GGGCGTGGGG AGCCTCACGG CGGCTCAGCA TTACCGTCTC
    AAAGCCAAAT ACGCCGCGGC CACGGGGGGG CCGCCCCCAA CTCCGCTGCC CGGCGTGGAG
    CCGCCGAACG TGGGCGGCCA CGGCGGTTTG CCCGGGGAGT GCCTCGAGCC GGCCGTGCCC
    TCCTTCTTCG CGAACGGTTT GCTGCGAAGG CACAGCTCCA ACGACCGCTA CGTGAGCAGC
    CTTCACCCTC ACCTGGCGCC TGGCAACGAC GTGAGGAGGG CCAGCGACCC GGCCCGGATT
    GCAGCCAACC CTCACTCTGT GCCCAAAGTG CAGCGTTTTA AAAGCGTGGG GAACGTGAAT
    GCGCCGGGAG GCGGCAGAGC TGCCCTGCAG CCCCTGGGTG CTTCCGACGG CAACCTGCAG
    CGCCGCGTCT TCTCCCCTCA CCCACCCAGC ATCAGTGAGA ATGTTTACCT GGAGAGCGTG
    AACCCGGACG GCCCCGGGCC TGGCGCGGGG TCTGGCTTGC TGGAAATGGA CCCGTTCTTG
    AACTACCCCG AGGAAAGCTT CCCGTGCCAG GGGGCAAACG TGCAGCTGCA GTGCGGGGGT
    CTGTATGGCA CCGCTCAGCG GACCCCGGGG GGCGGAATGC AGCTGAACCC GGGTGGGCAC
    AGGGAGATGG AGGAGGGATT GCTGCAGCCT GAGTTCTCCC TCCCGCAGTG CCAGATGAAC
    CAGCACTTCC ACGGCCTGCG TGGCGGCAGC GGGACCGTGC TGCCTCAATG GGAGGAGCCC
    CCCCAAAGCG ATCACCTGGA GATGGGCTCC GGGCAGCCAG CAGTGAGCGC CGTGCCTGAG
    ACGCACCGCC ACCGTCACAG CGCTCTGTAC CCACCGCCCA ACCACTGCGT GCAGCACCCC
    AAACCGATGA GCACGGGCCA CGATGGTGGC TTTTCAGATG GCCGTCGCTT GAACTGCCTG
    CAGATCAAAT CTGAGCAGCG GTACTCGGCT CCTGCCCCGG CCCTCACCCC GTGCCACGAT
    GCCAAAGCCA CGCGGCCGCC CGCGTTCGGC CGGGCTGTGA GTGGGTACCA GGCTGAGGAG
    CAGCCAGCCG GCGGCTACGC GGGCGTGCTG AGCCCACGGG GGAGGAGAGC CCACACCCCC
    ACTTCGCAGG CCAAAGAGGT GATGGTCCGC AGCTACGTGC AGGCGCAGCA GGCCCTGATG
    TGGGGAGAGC CCCCCAAGGG CGGTGAGGCC GGCGTGGGGC TGGGCGGTGA GTCTGGGCAG
    TACCAGGGCA CGCACGCAGC GCCGTACCTT GGCCCCAAGT ACTCTGGTTA CCAGCCCAAA
    GCCAACCACC TGCAGGGGCT GCTGGAGAAC CCCCACCTCC TGAGCCCCCC GTGCTTTAAC
    CCTGAGATGG TGCCACATCC CCCCAGCGGT GCCAAGCCAC CGGGCCACCA GAACAGCGTG
    GGCTATGGGG GCGACCTGGC AGATCCCATC CCCTCCTACG AGGCAGCGGG TGCCAACCCC
    CAGCGCGGGC TGCGCCTGCC CCCGGTCCAC CCTGCCCCCG AAGTGCCCGG TGATACACTG
    CTCTGCTACC CGGGCCAGGT GGGCAAAGGC AGCCACAAGC TTCAGGGTCC GCCTGCCGCA
    AGCTGTGGGG GTCCCGGGCA CTGCACTGGG AGCCTGGGGG GCAGCAAAGG CAGCTCTTGT
    TTCTACCCAG ACTCGGGGCA GCAGGCATTC AACAGCCTGG ACTCGCTGGA CCTGGAGAAC
    ACTCACCTGG ACTTTGCTGC CATCGTGGAG GATGCAGAGA GCCCCACGCT CCTGCCCGGC
    CCCCCCAGCC CCACTGGGGG TCTCCTGCTG CCAGCGCCCG GCGGTGCCAA CATGGCCGTG
    GGTGACATGA GCTCCATGCT GAGCACTTTG GCTGGGGAGA GCCACTTCCT CAACTCCCTC
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001048.1
    CDS623..4468
    Translation

    Target ORF information:

    RefSeq Version XM_025145280.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145280.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCAACC CCGTCACGCC TCAGGCCCCC GGCTACGCCG AGCACTGCTG CCCGCGGCCG 
    CTGCACGGAG CCTCTGCGGG CACACCGGGG CTGCAAGGAC TCGATTTCCC AGTGTGCCAT
    CAGCCCAACC TGGCCAGCAG CCACCATGGC TATGGGCTGG TGCCCGGCAC TGAGCACCCG
    GGTGGTGCTG CTGATGGCTC GCGGTTCTCG ACGCCCCGTG GTGCCGGCAA GCTGGGCAAG
    AAGCGGGCAC TGTCCATCTC ACCCCTGTCA GACTCTAGCG TTGACCTGCA GACGGTGATC
    CGCACCTCGC CCAACTCCCT CGTTGCCTTC ATCAACTCCC GTTGCGCTTC CGCCGGTGGC
    TCCTATGGTC ACCTCTCCAT CAGCACCATC AGTCCATCGT TGGGGTACCA GAACCCTCCG
    GGGCAGCAGA AGGGCCAAGG GCAACTGTTT AGCCACACTC CACCCCTGCC ACCGTGTAGC
    TCCCACGAAA CCCTGTCCTC CCGCCCAGGG CTCCTGCATC CCACCCCGGC TCGTGGGACC
    ATCAAACACT GCCAGCAGCT AAAGTTGGAG AGGAGCCTGA GTAGCCCTCT GACAGCCAAA
    TACCCAGAGG AGAAATCGGA GGGTGACATT TCCAGTCCGG CCTCCACCGG CACACAGGAC
    CCCTTGCTGG GGATGCTCAG CGTTCGGGAT GATCTGGAGA AGGAGGATGG GAAACCTGAA
    TCGGAGACCA TCTATGAAAC AAACTGCTAC TGGGATGGCT GCGCCAAGGA GTTTGACACG
    CAGGAGCAGC TGGTGCATCA CATCAACAAC GAGCACATCC ACGGGGAGAA GAAGGAGTTT
    GTGTGCCACT GGGCAGCGTG CTCCCGCGAG CAGCGGCCCT TCAAGGCTCA GTACATGCTG
    GTGGTGCACA TGAGGCGCCA CACGGGCGAG AAGCCTCACA AGTGCACGTT TGAGGGCTGT
    AACAAAGCGT ACTCCCGCCT GGAGAACCTC AAGACCCATC TGCGCTCCCA CACGGGTGAG
    AAACCCTATG TGTGTGAGCA CGAGGGTTGT AACAAAGCCT TCTCCAACGC TTCCGACCGG
    GCCAAGCATC AGAACCGCAC CCACTCCAAT GAGAAGCCAT ACATCTGTAA GATCCCGGGC
    TGCACCAAGC GCTACACGGA TCCCAGCTCC CTGCGCAAAC ACGTGAAGAC GGTGCACGGC
    CCCGACGCCC ACGTCACCAA GAAGCACCGG GGCAGCGTTG TGCCTGGCCA TGCACTGCCT
    GCCTCTGCTG CACCCCAGGA CATGAAGCAG GAGAAAAACA CCAACGGCCC TGCTGAGATC
    CGCAAGGATG ACGGCAAGCT GCTGGTGCCT GACTTGGTGT CGAAGCCACA GCCCAGCCCT
    GGTGGGCAGT CATCGTGCAG CAGTGACCGC TCCCCCCTCG GCAGCACCAC CAACAACGAC
    AGCGGTGTGG AGATGACGGG CAACACGGGT GGCAGCTACG AGGATCTGTC CACGCTGGAG
    GATGTGGTGC CCGGGGATCC CATGGGCACC TCGGGGCTGA TGGCCCTCCA GAAGCTCGAG
    AACCTTCGCA TTGACAAACT GAAGCAGATG AGGAAGCCAT CAACCAAGGG CCTGAACCTG
    CCAGCCATCC CCGGAGCCGG TCTGCCCGGA GATGTGCCCA GGGTCTGCGT GCCGCCGCCG
    GCTGCGTCCC AGCGCCGCGT CGTGGAGCTG TCGGCTGCAG AGGTGGCCGT TCCGCCGAGC
    GAGCGCCGCA GCAGCGCCAC CAGCACCATC AGCTCAGCCT ACACCGTCAG CCGCCGCTCC
    TCCGCCGCCT CCCCGTACCT ACCGGGGCCG AACTTGCCCG GCGAGGGGGG GACGATGCCG
    GCACGCTCGG ATGGCTACGA CTGCATCTCT CCGGATGAGC TGCGGCGCTC CGGCAACGCC
    GATCCCTGCG GGGCGCTGCC GGGCGTGGGG AGCCTCACGG CGGCTCAGCA TTACCGTCTC
    AAAGCCAAAT ACGCCGCGGC CACGGGGGGG CCGCCCCCAA CTCCGCTGCC CGGCGTGGAG
    CCGCCGAACG TGGGCGGCCA CGGCGGTTTG CCCGGGGAGT GCCTCGAGCC GGCCGTGCCC
    TCCTTCTTCG CGAACGGTTT GCTGCGAAGG CACAGCTCCA ACGACCGCTA CGTGAGCAGC
    CTTCACCCTC ACCTGGCGCC TGGCAACGAC GTGAGGAGGG CCAGCGACCC GGCCCGGATT
    GCAGCCAACC CTCACTCTGT GCCCAAAGTG CAGCGTTTTA AAAGCGTGGG GAACGTGAAT
    GCGCCGGGAG GCGGCAGAGC TGCCCTGCAG CCCCTGGGTG CTTCCGACGG CAACCTGCAG
    CGCCGCGTCT TCTCCCCTCA CCCACCCAGC ATCAGTGAGA ATGTTTACCT GGAGAGCGTG
    AACCCGGACG GCCCCGGGCC TGGCGCGGGG TCTGGCTTGC TGGAAATGGA CCCGTTCTTG
    AACTACCCCG AGGAAAGCTT CCCGTGCCAG GGGGCAAACG TGCAGCTGCA GTGCGGGGGT
    CTGTATGGCA CCGCTCAGCG GACCCCGGGG GGCGGAATGC AGCTGAACCC GGGTGGGCAC
    AGGGAGATGG AGGAGGGATT GCTGCAGCCT GAGTTCTCCC TCCCGCAGTG CCAGATGAAC
    CAGCACTTCC ACGGCCTGCG TGGCGGCAGC GGGACCGTGC TGCCTCAATG GGAGGAGCCC
    CCCCAAAGCG ATCACCTGGA GATGGGCTCC GGGCAGCCAG CAGTGAGCGC CGTGCCTGAG
    ACGCACCGCC ACCGTCACAG CGCTCTGTAC CCACCGCCCA ACCACTGCGT GCAGCACCCC
    AAACCGATGA GCACGGGCCA CGATGGTGGC TTTTCAGATG GCCGTCGCTT GAACTGCCTG
    CAGATCAAAT CTGAGCAGCG GTACTCGGCT CCTGCCCCGG CCCTCACCCC GTGCCACGAT
    GCCAAAGCCA CGCGGCCGCC CGCGTTCGGC CGGGCTGTGA GTGGGTACCA GGCTGAGGAG
    CAGCCAGCCG GCGGCTACGC GGGCGTGCTG AGCCCACGGG GGAGGAGAGC CCACACCCCC
    ACTTCGCAGG CCAAAGAGGT GATGGTCCGC AGCTACGTGC AGGCGCAGCA GGCCCTGATG
    TGGGGAGAGC CCCCCAAGGG CGGTGAGGCC GGCGTGGGGC TGGGCGGTGA GTCTGGGCAG
    TACCAGGGCA CGCACGCAGC GCCGTACCTT GGCCCCAAGT ACTCTGGTTA CCAGCCCAAA
    GCCAACCACC TGCAGGGGCT GCTGGAGAAC CCCCACCTCC TGAGCCCCCC GTGCTTTAAC
    CCTGAGATGG TGCCACATCC CCCCAGCGGT GCCAAGCCAC CGGGCCACCA GAACAGCGTG
    GGCTATGGGG GCGACCTGGC AGATCCCATC CCCTCCTACG AGGCAGCGGG TGCCAACCCC
    CAGCGCGGGC TGCGCCTGCC CCCGGTCCAC CCTGCCCCCG AAGTGCCCGG TGATACACTG
    CTCTGCTACC CGGGCCAGGT GGGCAAAGGC AGCCACAAGC TTCAGGGTCC GCCTGCCGCA
    AGCTGTGGGG GTCCCGGGCA CTGCACTGGG AGCCTGGGGG GCAGCAAAGG CAGCTCTTGT
    TTCTACCCAG ACTCGGGGCA GCAGGCATTC AACAGCCTGG ACTCGCTGGA CCTGGAGAAC
    ACTCACCTGG ACTTTGCTGC CATCGTGGAG GATGCAGAGA GCCCCACGCT CCTGCCCGGC
    CCCCCCAGCC CCACTGGGGG TCTCCTGCTG CCAGCGCCCG GCGGTGCCAA CATGGCCGTG
    GGTGACATGA GCTCCATGCT GAGCACTTTG GCTGGGGAGA GCCACTTCCT CAACTCCCTC
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149630
    Clone ID Related Accession (Same CDS sequence) XM_025145281.1
    Accession Version XM_025145281.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein GLI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008465.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCAACC CCGTCACGCC TCAGGCCCCC GGCTACGCCG AGCACTGCTG CCCGCGGCCG 
    CTGCACGGAG CCTCTGCGGG CACACCGGGG CTGCAAGGAC TCGATTTCCC AGTGTGCCAT
    CAGCCCAACC TGGCCAGCAG CCACCATGGC TATGGGCTGG TGCCCGGCAC TGAGCACCCG
    GGTGGTGCTG CTGATGGCTC GCGGTTCTCG ACGCCCCGTG GTGCCGGCAA GCTGGGCAAG
    AAGCGGGCAC TGTCCATCTC ACCCCTGTCA GACTCTAGCG TTGACCTGCA GACGGTGATC
    CGCACCTCGC CCAACTCCCT CGTTGCCTTC ATCAACTCCC GTTGCGCTTC CGCCGGTGGC
    TCCTATGGTC ACCTCTCCAT CAGCACCATC AGTCCATCGT TGGGGTACCA GAACCCTCCG
    GGGCAGCAGA AGGGCCAAGG GCAACTGTTT AGCCACACTC CACCCCTGCC ACCGTGTAGC
    TCCCACGAAA CCCTGTCCTC CCGCCCAGGG CTCCTGCATC CCACCCCGGC TCGTGGGACC
    ATCAAACACT GCCAGCTAAA GTTGGAGAGG AGCCTGAGTA GCCCTCTGAC AGCCAAATAC
    CCAGAGGAGA AATCGGAGGG TGACATTTCC AGTCCGGCCT CCACCGGCAC ACAGGACCCC
    TTGCTGGGGA TGCTCAGCGT TCGGGATGAT CTGGAGAAGG AGGATGGGAA ACCTGAATCG
    GAGACCATCT ATGAAACAAA CTGCTACTGG GATGGCTGCG CCAAGGAGTT TGACACGCAG
    GAGCAGCTGG TGCATCACAT CAACAACGAG CACATCCACG GGGAGAAGAA GGAGTTTGTG
    TGCCACTGGG CAGCGTGCTC CCGCGAGCAG CGGCCCTTCA AGGCTCAGTA CATGCTGGTG
    GTGCACATGA GGCGCCACAC GGGCGAGAAG CCTCACAAGT GCACGTTTGA GGGCTGTAAC
    AAAGCGTACT CCCGCCTGGA GAACCTCAAG ACCCATCTGC GCTCCCACAC GGGTGAGAAA
    CCCTATGTGT GTGAGCACGA GGGTTGTAAC AAAGCCTTCT CCAACGCTTC CGACCGGGCC
    AAGCATCAGA ACCGCACCCA CTCCAATGAG AAGCCATACA TCTGTAAGAT CCCGGGCTGC
    ACCAAGCGCT ACACGGATCC CAGCTCCCTG CGCAAACACG TGAAGACGGT GCACGGCCCC
    GACGCCCACG TCACCAAGAA GCACCGGGGC AGCGTTGTGC CTGGCCATGC ACTGCCTGCC
    TCTGCTGCAC CCCAGGACAT GAAGCAGGAG AAAAACACCA ACGGCCCTGC TGAGATCCGC
    AAGGATGACG GCAAGCTGCT GGTGCCTGAC TTGGTGTCGA AGCCACAGCC CAGCCCTGGT
    GGGCAGTCAT CGTGCAGCAG TGACCGCTCC CCCCTCGGCA GCACCACCAA CAACGACAGC
    GGTGTGGAGA TGACGGGCAA CACGGGTGGC AGCTACGAGG ATCTGTCCAC GCTGGAGGAT
    GTGGTGCCCG GGGATCCCAT GGGCACCTCG GGGCTGATGG CCCTCCAGAA GCTCGAGAAC
    CTTCGCATTG ACAAACTGAA GCAGATGAGG AAGCCATCAA CCAAGGGCCT GAACCTGCCA
    GCCATCCCCG GAGCCGGTCT GCCCGGAGAT GTGCCCAGGG TCTGCGTGCC GCCGCCGGCT
    GCGTCCCAGC GCCGCGTCGT GGAGCTGTCG GCTGCAGAGG TGGCCGTTCC GCCGAGCGAG
    CGCCGCAGCA GCGCCACCAG CACCATCAGC TCAGCCTACA CCGTCAGCCG CCGCTCCTCC
    GCCGCCTCCC CGTACCTACC GGGGCCGAAC TTGCCCGGCG AGGGGGGGAC GATGCCGGCA
    CGCTCGGATG GCTACGACTG CATCTCTCCG GATGAGCTGC GGCGCTCCGG CAACGCCGAT
    CCCTGCGGGG CGCTGCCGGG CGTGGGGAGC CTCACGGCGG CTCAGCATTA CCGTCTCAAA
    GCCAAATACG CCGCGGCCAC GGGGGGGCCG CCCCCAACTC CGCTGCCCGG CGTGGAGCCG
    CCGAACGTGG GCGGCCACGG CGGTTTGCCC GGGGAGTGCC TCGAGCCGGC CGTGCCCTCC
    TTCTTCGCGA ACGGTTTGCT GCGAAGGCAC AGCTCCAACG ACCGCTACGT GAGCAGCCTT
    CACCCTCACC TGGCGCCTGG CAACGACGTG AGGAGGGCCA GCGACCCGGC CCGGATTGCA
    GCCAACCCTC ACTCTGTGCC CAAAGTGCAG CGTTTTAAAA GCGTGGGGAA CGTGAATGCG
    CCGGGAGGCG GCAGAGCTGC CCTGCAGCCC CTGGGTGCTT CCGACGGCAA CCTGCAGCGC
    CGCGTCTTCT CCCCTCACCC ACCCAGCATC AGTGAGAATG TTTACCTGGA GAGCGTGAAC
    CCGGACGGCC CCGGGCCTGG CGCGGGGTCT GGCTTGCTGG AAATGGACCC GTTCTTGAAC
    TACCCCGAGG AAAGCTTCCC GTGCCAGGGG GCAAACGTGC AGCTGCAGTG CGGGGGTCTG
    TATGGCACCG CTCAGCGGAC CCCGGGGGGC GGAATGCAGC TGAACCCGGG TGGGCACAGG
    GAGATGGAGG AGGGATTGCT GCAGCCTGAG TTCTCCCTCC CGCAGTGCCA GATGAACCAG
    CACTTCCACG GCCTGCGTGG CGGCAGCGGG ACCGTGCTGC CTCAATGGGA GGAGCCCCCC
    CAAAGCGATC ACCTGGAGAT GGGCTCCGGG CAGCCAGCAG TGAGCGCCGT GCCTGAGACG
    CACCGCCACC GTCACAGCGC TCTGTACCCA CCGCCCAACC ACTGCGTGCA GCACCCCAAA
    CCGATGAGCA CGGGCCACGA TGGTGGCTTT TCAGATGGCC GTCGCTTGAA CTGCCTGCAG
    ATCAAATCTG AGCAGCGGTA CTCGGCTCCT GCCCCGGCCC TCACCCCGTG CCACGATGCC
    AAAGCCACGC GGCCGCCCGC GTTCGGCCGG GCTGTGAGTG GGTACCAGGC TGAGGAGCAG
    CCAGCCGGCG GCTACGCGGG CGTGCTGAGC CCACGGGGGA GGAGAGCCCA CACCCCCACT
    TCGCAGGCCA AAGAGGTGAT GGTCCGCAGC TACGTGCAGG CGCAGCAGGC CCTGATGTGG
    GGAGAGCCCC CCAAGGGCGG TGAGGCCGGC GTGGGGCTGG GCGGTGAGTC TGGGCAGTAC
    CAGGGCACGC ACGCAGCGCC GTACCTTGGC CCCAAGTACT CTGGTTACCA GCCCAAAGCC
    AACCACCTGC AGGGGCTGCT GGAGAACCCC CACCTCCTGA GCCCCCCGTG CTTTAACCCT
    GAGATGGTGC CACATCCCCC CAGCGGTGCC AAGCCACCGG GCCACCAGAA CAGCGTGGGC
    TATGGGGGCG ACCTGGCAGA TCCCATCCCC TCCTACGAGG CAGCGGGTGC CAACCCCCAG
    CGCGGGCTGC GCCTGCCCCC GGTCCACCCT GCCCCCGAAG TGCCCGGTGA TACACTGCTC
    TGCTACCCGG GCCAGGTGGG CAAAGGCAGC CACAAGCTTC AGGGTCCGCC TGCCGCAAGC
    TGTGGGGGTC CCGGGCACTG CACTGGGAGC CTGGGGGGCA GCAAAGGCAG CTCTTGTTTC
    TACCCAGACT CGGGGCAGCA GGCATTCAAC AGCCTGGACT CGCTGGACCT GGAGAACACT
    CACCTGGACT TTGCTGCCAT CGTGGAGGAT GCAGAGAGCC CCACGCTCCT GCCCGGCCCC
    CCCAGCCCCA CTGGGGGTCT CCTGCTGCCA GCGCCCGGCG GTGCCAACAT GGCCGTGGGT
    GACATGAGCT CCATGCTGAG CACTTTGGCT GGGGAGAGCC ACTTCCTCAA CTCCCTCTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001049.1
    CDS664..4506
    Translation

    Target ORF information:

    RefSeq Version XM_025145281.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145281.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCAACC CCGTCACGCC TCAGGCCCCC GGCTACGCCG AGCACTGCTG CCCGCGGCCG 
    CTGCACGGAG CCTCTGCGGG CACACCGGGG CTGCAAGGAC TCGATTTCCC AGTGTGCCAT
    CAGCCCAACC TGGCCAGCAG CCACCATGGC TATGGGCTGG TGCCCGGCAC TGAGCACCCG
    GGTGGTGCTG CTGATGGCTC GCGGTTCTCG ACGCCCCGTG GTGCCGGCAA GCTGGGCAAG
    AAGCGGGCAC TGTCCATCTC ACCCCTGTCA GACTCTAGCG TTGACCTGCA GACGGTGATC
    CGCACCTCGC CCAACTCCCT CGTTGCCTTC ATCAACTCCC GTTGCGCTTC CGCCGGTGGC
    TCCTATGGTC ACCTCTCCAT CAGCACCATC AGTCCATCGT TGGGGTACCA GAACCCTCCG
    GGGCAGCAGA AGGGCCAAGG GCAACTGTTT AGCCACACTC CACCCCTGCC ACCGTGTAGC
    TCCCACGAAA CCCTGTCCTC CCGCCCAGGG CTCCTGCATC CCACCCCGGC TCGTGGGACC
    ATCAAACACT GCCAGCTAAA GTTGGAGAGG AGCCTGAGTA GCCCTCTGAC AGCCAAATAC
    CCAGAGGAGA AATCGGAGGG TGACATTTCC AGTCCGGCCT CCACCGGCAC ACAGGACCCC
    TTGCTGGGGA TGCTCAGCGT TCGGGATGAT CTGGAGAAGG AGGATGGGAA ACCTGAATCG
    GAGACCATCT ATGAAACAAA CTGCTACTGG GATGGCTGCG CCAAGGAGTT TGACACGCAG
    GAGCAGCTGG TGCATCACAT CAACAACGAG CACATCCACG GGGAGAAGAA GGAGTTTGTG
    TGCCACTGGG CAGCGTGCTC CCGCGAGCAG CGGCCCTTCA AGGCTCAGTA CATGCTGGTG
    GTGCACATGA GGCGCCACAC GGGCGAGAAG CCTCACAAGT GCACGTTTGA GGGCTGTAAC
    AAAGCGTACT CCCGCCTGGA GAACCTCAAG ACCCATCTGC GCTCCCACAC GGGTGAGAAA
    CCCTATGTGT GTGAGCACGA GGGTTGTAAC AAAGCCTTCT CCAACGCTTC CGACCGGGCC
    AAGCATCAGA ACCGCACCCA CTCCAATGAG AAGCCATACA TCTGTAAGAT CCCGGGCTGC
    ACCAAGCGCT ACACGGATCC CAGCTCCCTG CGCAAACACG TGAAGACGGT GCACGGCCCC
    GACGCCCACG TCACCAAGAA GCACCGGGGC AGCGTTGTGC CTGGCCATGC ACTGCCTGCC
    TCTGCTGCAC CCCAGGACAT GAAGCAGGAG AAAAACACCA ACGGCCCTGC TGAGATCCGC
    AAGGATGACG GCAAGCTGCT GGTGCCTGAC TTGGTGTCGA AGCCACAGCC CAGCCCTGGT
    GGGCAGTCAT CGTGCAGCAG TGACCGCTCC CCCCTCGGCA GCACCACCAA CAACGACAGC
    GGTGTGGAGA TGACGGGCAA CACGGGTGGC AGCTACGAGG ATCTGTCCAC GCTGGAGGAT
    GTGGTGCCCG GGGATCCCAT GGGCACCTCG GGGCTGATGG CCCTCCAGAA GCTCGAGAAC
    CTTCGCATTG ACAAACTGAA GCAGATGAGG AAGCCATCAA CCAAGGGCCT GAACCTGCCA
    GCCATCCCCG GAGCCGGTCT GCCCGGAGAT GTGCCCAGGG TCTGCGTGCC GCCGCCGGCT
    GCGTCCCAGC GCCGCGTCGT GGAGCTGTCG GCTGCAGAGG TGGCCGTTCC GCCGAGCGAG
    CGCCGCAGCA GCGCCACCAG CACCATCAGC TCAGCCTACA CCGTCAGCCG CCGCTCCTCC
    GCCGCCTCCC CGTACCTACC GGGGCCGAAC TTGCCCGGCG AGGGGGGGAC GATGCCGGCA
    CGCTCGGATG GCTACGACTG CATCTCTCCG GATGAGCTGC GGCGCTCCGG CAACGCCGAT
    CCCTGCGGGG CGCTGCCGGG CGTGGGGAGC CTCACGGCGG CTCAGCATTA CCGTCTCAAA
    GCCAAATACG CCGCGGCCAC GGGGGGGCCG CCCCCAACTC CGCTGCCCGG CGTGGAGCCG
    CCGAACGTGG GCGGCCACGG CGGTTTGCCC GGGGAGTGCC TCGAGCCGGC CGTGCCCTCC
    TTCTTCGCGA ACGGTTTGCT GCGAAGGCAC AGCTCCAACG ACCGCTACGT GAGCAGCCTT
    CACCCTCACC TGGCGCCTGG CAACGACGTG AGGAGGGCCA GCGACCCGGC CCGGATTGCA
    GCCAACCCTC ACTCTGTGCC CAAAGTGCAG CGTTTTAAAA GCGTGGGGAA CGTGAATGCG
    CCGGGAGGCG GCAGAGCTGC CCTGCAGCCC CTGGGTGCTT CCGACGGCAA CCTGCAGCGC
    CGCGTCTTCT CCCCTCACCC ACCCAGCATC AGTGAGAATG TTTACCTGGA GAGCGTGAAC
    CCGGACGGCC CCGGGCCTGG CGCGGGGTCT GGCTTGCTGG AAATGGACCC GTTCTTGAAC
    TACCCCGAGG AAAGCTTCCC GTGCCAGGGG GCAAACGTGC AGCTGCAGTG CGGGGGTCTG
    TATGGCACCG CTCAGCGGAC CCCGGGGGGC GGAATGCAGC TGAACCCGGG TGGGCACAGG
    GAGATGGAGG AGGGATTGCT GCAGCCTGAG TTCTCCCTCC CGCAGTGCCA GATGAACCAG
    CACTTCCACG GCCTGCGTGG CGGCAGCGGG ACCGTGCTGC CTCAATGGGA GGAGCCCCCC
    CAAAGCGATC ACCTGGAGAT GGGCTCCGGG CAGCCAGCAG TGAGCGCCGT GCCTGAGACG
    CACCGCCACC GTCACAGCGC TCTGTACCCA CCGCCCAACC ACTGCGTGCA GCACCCCAAA
    CCGATGAGCA CGGGCCACGA TGGTGGCTTT TCAGATGGCC GTCGCTTGAA CTGCCTGCAG
    ATCAAATCTG AGCAGCGGTA CTCGGCTCCT GCCCCGGCCC TCACCCCGTG CCACGATGCC
    AAAGCCACGC GGCCGCCCGC GTTCGGCCGG GCTGTGAGTG GGTACCAGGC TGAGGAGCAG
    CCAGCCGGCG GCTACGCGGG CGTGCTGAGC CCACGGGGGA GGAGAGCCCA CACCCCCACT
    TCGCAGGCCA AAGAGGTGAT GGTCCGCAGC TACGTGCAGG CGCAGCAGGC CCTGATGTGG
    GGAGAGCCCC CCAAGGGCGG TGAGGCCGGC GTGGGGCTGG GCGGTGAGTC TGGGCAGTAC
    CAGGGCACGC ACGCAGCGCC GTACCTTGGC CCCAAGTACT CTGGTTACCA GCCCAAAGCC
    AACCACCTGC AGGGGCTGCT GGAGAACCCC CACCTCCTGA GCCCCCCGTG CTTTAACCCT
    GAGATGGTGC CACATCCCCC CAGCGGTGCC AAGCCACCGG GCCACCAGAA CAGCGTGGGC
    TATGGGGGCG ACCTGGCAGA TCCCATCCCC TCCTACGAGG CAGCGGGTGC CAACCCCCAG
    CGCGGGCTGC GCCTGCCCCC GGTCCACCCT GCCCCCGAAG TGCCCGGTGA TACACTGCTC
    TGCTACCCGG GCCAGGTGGG CAAAGGCAGC CACAAGCTTC AGGGTCCGCC TGCCGCAAGC
    TGTGGGGGTC CCGGGCACTG CACTGGGAGC CTGGGGGGCA GCAAAGGCAG CTCTTGTTTC
    TACCCAGACT CGGGGCAGCA GGCATTCAAC AGCCTGGACT CGCTGGACCT GGAGAACACT
    CACCTGGACT TTGCTGCCAT CGTGGAGGAT GCAGAGAGCC CCACGCTCCT GCCCGGCCCC
    CCCAGCCCCA CTGGGGGTCT CCTGCTGCCA GCGCCCGGCG GTGCCAACAT GGCCGTGGGT
    GACATGAGCT CCATGCTGAG CACTTTGGCT GGGGAGAGCC ACTTCCTCAA CTCCCTCTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa23251
    Clone ID Related Accession (Same CDS sequence) NM_001305245.1
    Accession Version NM_001305245.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-25
    Organism Gallus gallus(chicken)
    Product zinc finger protein GLI1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN03016051.1. On Mar 5, 2015 this sequence version replaced XM_413682.3. ##Evidence-Data-START## Transcript exon combination :: U60762.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SRS380260 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCAACC CCGTCACGCC TCAGGCCCCC GGCTACGCCG AGCACTGCTG CCCGCGGCCG 
    CTGCATGGAG CCTCTGCGGG CACACCGGGG CTGCAAGGAC TCGATTTCCC AGTGTGCCAT
    CAGCCCAACC TGGCCAGCAG CCACCATGGC TATGGGCTGG TGCCCGGCAC TGAGCATCCG
    GGTGGTGCTG CTGATGGCTC GCGGTTCTCG ACGCCCCGTG GTGCCGGCAA GCTGGGCAAG
    AAGCGGGCAC TGTCCATCTC ACCCCTGTCA GACTCTAGCG TTGACCTGCA GACGGTGATC
    CGCACCTCGC CCAACTCCCT CGTTGCCTTC ATCAACTCCC GTTGCGCTTC CGCCGGTGGC
    TCCTATGGTC ACCTCTCCAT CAGCACCATC AGTCCATCGT TGGGGTACCA GAACCCTCCG
    GGGCAGCAGA AGGGCCAAGG GCAACTGTTT AGCCACACTC CACCCCTGCC ACCGTGTAGC
    TCCCACGAAA CCCTGTCCTC CCGCCCAGGG CTCCTGCATC CCACCCCGGC TCGTGGGACC
    ATCAAACACT GCCAGCAGCT AAAGTTGGAG AGGAGCCTGA GTAGCCCTCT GACAGCCAAA
    TACCCAGAGG AGAAATCGGA GGGTGACATT TCCAGTCCGG CCTCCACCGG CACACAGGAC
    CCCTTGCTGG GGATGCTCAG CGTTCGGGAT GATCTGGAGA AGGAGGATGG GAAACCTGAA
    TCGGAGACCA TCTATGAAAC AAACTGCTAC TGGGATGGCT GCGCCAAGGA GTTTGACACG
    CAGGAGCAGC TGGTGCATCA CATCAACAAC GAGCACATCC ACGGGGAGAA GAAGGAGTTT
    GTGTGCCACT GGGCAGCGTG CTCCCGCGAG CAGCGGCCCT TCAAGGCTCA GTACATGCTG
    GTGGTGCACA TGAGGCGCCA CACGGGCGAG AAGCCTCACA AGTGCACGTT TGAGGGCTGT
    AACAAAGCGT ACTCCCGCCT GGAGAACCTC AAGACCCATC TGCGCTCCCA CACGGGTGAG
    AAACCCTATG TGTGTGAGCA CGAGGGTTGT AACAAAGCCT TCTCCAACGC TTCCGACCGG
    GCCAAGCATC AGAACCGCAC CCACTCCAAT GAGAAGCCAT ACATCTGTAA GATCCCGGGC
    TGCACCAAGC GCTACACGGA TCCCAGCTCC CTGCGCAAAC ACGTGAAGAC GGTGCACGGC
    CCCGACGCCC ACGTCACCAA GAAGCACCGG GGCAGCGTTG TGCCTGGCCA TGCACTGCCT
    GCCTCTGCTG CACCCCAGGA CATGAAGCAG GAGAAAAACA CCAACGGCCC TGCTGAGATC
    CGCAAGGATG ACGGCAAGCT GCTGGTGCCT GACTTGGTGT CGAAGCCACA GCCCAGCCCT
    GGTGGGCAGT CATCGTGCAG CAGTGACCGC TCCCCCCTCG GCAGCACCAC CAACAACGAC
    AGCGGTGTGG AGATGACGGG CAACACGGGT GGCAGCTACG AGGATCTGTC CACGCTGGAG
    GATGTGGTGC CCGGGGATCC CATGGGCACC TCGGGGCTGA TGGCCCTCCA GAAGCTCGAG
    AACCTTCGCA TTGACAAACT GAAGCAGATG AGGAAGCCAT CAACCAAGGG CCTGAACCTG
    CCAGCCATCC CCGGAGCCGG TCTGCCCGGA GATGTGCCCA GGGTCTGCGT GCCGCCGCCG
    GCTGCGTCCC AGCGCCGCGT CGTGGAGCTG TCGGCTGCAG AGGTGGCCGT TCCGCCGAGC
    GAGCGCCGCA GCAGCGCCAC CAGCACCATC AGCTCAGCCT ACACCGTCAG CCGCCGCTCC
    TCCGCCGCCT CCCCGTACCT ACCGGGGCCG AACCTGCCCG GCGAGGGGGG GACGATGCCG
    GCACGCTCGG ATGGCTACGA CTGCATCTCT CCGGATGAGC TGCGGCGCTC CGGCAACGCC
    GATCCCTGCG GGGCGCTGCC GGGCGTGGGG AGCCTCACGG CGGCTCAGCA TTACCGTCTC
    AAAGCCAAAT ACGCCGCGGC CACGGGGGGG CCGCCCCCAA CTCCGCTGCC CGGCGTGGAG
    CCGCCGAACG TGGGCGGCCA CGGCGGTTTG CCCGGGGAGT GCCTCGAGCC GGCCGTGCCC
    TCCTTCTTCG CGAACGGTTT GCTGCGAAGG CACAGCTCCA ACGACCGCTA CGTGAGCAGC
    CTTCACCCTC ACCTGGCGCC TGGCAACGAC GTGAGGAGGG CCAGCGACCC GGCCCGGATT
    GCAGCCAACC CTCACTCTGT GCCCAAAGTG CAGCGTTTTA AAAGCGTGGG GAACGTGAAT
    GCGCCGGGAG GCGGCAGAGC TGCCCTGCAG CCCCTGGGTG CTTCCGACGG CAACCTGCAG
    CGCCGCGTCT TCTCCCCTCA CCCACCCAGC ATCAGTGAGA ATGTTTACCT GGAGAGCGTG
    AACCCGGACG GCCCCGGGCC TGGCGCGGGG TCTGGCTTGC TGGAAATGGA CCCGTTCTTG
    AACTACCCCG AGGAAAGCTT CCCGTGCCAG GGGGCAAACG TGCAGCTGCA GTGCGGGGGT
    CTGTATGGCA CCGCTCAGCG GACCCCGGGG GGCGGAATGC AGCTGAACCC GGGTGGGCAC
    AGGGAGATGG AGGAGGGATT GCTGCAGCCT GAGTTCTCCC TCCCGCAGTG CCAGATGAAC
    CAGCACTTCC ACGGCCTGCG TGGCGGCAGC GGGACCGTGC TGCCTCAATG GGAGGAGCCC
    CCCCAAAGCG ATCACCTGGA GATGGGCTCC GGGCAGCCAG CAGTGAGCGC CGTGCCTGAG
    ACGCACCGCC ACCGTCACAG CGCTCTGTAC CCACCGCCCA ACCACTGCGT GCAGCACCCC
    AAACCGATGA GCACGGGCCA CGATGGTGGC TTTTCAGATG GCCGTCGCTT GAACTGCCTG
    CAGATCAAAT CTGAGCAGCG GTACTCGGCT CCTGCCCCGG CCCTCACCCC GTGCCACGAT
    GCCAAAGCCA CGCGGCCGCC CGCGTTCGGC CGGGCTGTGA GTGGGTACCA GGCTGAGGAG
    CAGCCAGCCG GCGGCTACGC GGGCGTGCTG AGCCCACGGG GGAGGAGAGC CCACACCCCC
    ACTTCGCAGG CCAAAGAGGT GATGGTCCGC AGCTACGTGC AGGCGCAGCA GGCCCTGATG
    TGGGGAGAGC CCCCCAAGGG CGGTGAGGCC GGCGTGGGGC TGGGCGGTGA GTCTGGGCAG
    TACCAGGGCA CGCACGCAGC GCCGTACCTT GGCCCCAAGT ACTCTGGTTA CCAGCCCAAA
    GCCAACCACC TGCAGGGGCT GCTGGAGAAC CCCCACCTCC TGAGCCCCCC GTGCTTTAAC
    CCTGAGATGG TGCCACATCC CCCCAGCGGT GCCAAGCCAC CGGGCCACCA GAACAGCGTG
    GGCTATGGGG GCGACCTGGC AGATCCCATC CCCTCCTACG AGGCAGCGGG TGCCAACCCC
    CAGCGCGGGC TGCGCCTGCC CCCGGTCCAC CCTGCCCCCG AAGTGCCCGG TGATACACTG
    CTCTGCTACC CGGGCCAGGT GGGCAAAGGC AGCCACAAGC TTCAGGGTCC GCCTGCCGCA
    AGCTGTGGGG GTCCCGGGCA CTGCACTGGG AGCCTGGGGG GCAGCAAAGG CAGCTCTTGT
    TTCTACCCAG ACTCGGGGCA GCAGGCATTC AACAGCCTGG ACTCGCTGGA CCTGGAGAAC
    ACTCACCTGG ACTTTGCTGC CATCGTGGAG GATGCAGAGA GCCCCACGCT CCTGCCTGGC
    CCCCCCAGCC CCACTGGGGG TCTCCTGCTG CCAGCGCCCG GCGGTGCCAA CATGGCCGTG
    GGTGACATGA GCTCCATGCT GAGCACTTTG GCTGGGGAGA GCCACTTCCT CAACTCCCTC
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001292174.1
    CDS77..3922
    Translation

    Target ORF information:

    RefSeq Version NM_001305245.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 1 (GLI1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001305245.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCAACC CCGTCACGCC TCAGGCCCCC GGCTACGCCG AGCACTGCTG CCCGCGGCCG 
    CTGCATGGAG CCTCTGCGGG CACACCGGGG CTGCAAGGAC TCGATTTCCC AGTGTGCCAT
    CAGCCCAACC TGGCCAGCAG CCACCATGGC TATGGGCTGG TGCCCGGCAC TGAGCATCCG
    GGTGGTGCTG CTGATGGCTC GCGGTTCTCG ACGCCCCGTG GTGCCGGCAA GCTGGGCAAG
    AAGCGGGCAC TGTCCATCTC ACCCCTGTCA GACTCTAGCG TTGACCTGCA GACGGTGATC
    CGCACCTCGC CCAACTCCCT CGTTGCCTTC ATCAACTCCC GTTGCGCTTC CGCCGGTGGC
    TCCTATGGTC ACCTCTCCAT CAGCACCATC AGTCCATCGT TGGGGTACCA GAACCCTCCG
    GGGCAGCAGA AGGGCCAAGG GCAACTGTTT AGCCACACTC CACCCCTGCC ACCGTGTAGC
    TCCCACGAAA CCCTGTCCTC CCGCCCAGGG CTCCTGCATC CCACCCCGGC TCGTGGGACC
    ATCAAACACT GCCAGCAGCT AAAGTTGGAG AGGAGCCTGA GTAGCCCTCT GACAGCCAAA
    TACCCAGAGG AGAAATCGGA GGGTGACATT TCCAGTCCGG CCTCCACCGG CACACAGGAC
    CCCTTGCTGG GGATGCTCAG CGTTCGGGAT GATCTGGAGA AGGAGGATGG GAAACCTGAA
    TCGGAGACCA TCTATGAAAC AAACTGCTAC TGGGATGGCT GCGCCAAGGA GTTTGACACG
    CAGGAGCAGC TGGTGCATCA CATCAACAAC GAGCACATCC ACGGGGAGAA GAAGGAGTTT
    GTGTGCCACT GGGCAGCGTG CTCCCGCGAG CAGCGGCCCT TCAAGGCTCA GTACATGCTG
    GTGGTGCACA TGAGGCGCCA CACGGGCGAG AAGCCTCACA AGTGCACGTT TGAGGGCTGT
    AACAAAGCGT ACTCCCGCCT GGAGAACCTC AAGACCCATC TGCGCTCCCA CACGGGTGAG
    AAACCCTATG TGTGTGAGCA CGAGGGTTGT AACAAAGCCT TCTCCAACGC TTCCGACCGG
    GCCAAGCATC AGAACCGCAC CCACTCCAAT GAGAAGCCAT ACATCTGTAA GATCCCGGGC
    TGCACCAAGC GCTACACGGA TCCCAGCTCC CTGCGCAAAC ACGTGAAGAC GGTGCACGGC
    CCCGACGCCC ACGTCACCAA GAAGCACCGG GGCAGCGTTG TGCCTGGCCA TGCACTGCCT
    GCCTCTGCTG CACCCCAGGA CATGAAGCAG GAGAAAAACA CCAACGGCCC TGCTGAGATC
    CGCAAGGATG ACGGCAAGCT GCTGGTGCCT GACTTGGTGT CGAAGCCACA GCCCAGCCCT
    GGTGGGCAGT CATCGTGCAG CAGTGACCGC TCCCCCCTCG GCAGCACCAC CAACAACGAC
    AGCGGTGTGG AGATGACGGG CAACACGGGT GGCAGCTACG AGGATCTGTC CACGCTGGAG
    GATGTGGTGC CCGGGGATCC CATGGGCACC TCGGGGCTGA TGGCCCTCCA GAAGCTCGAG
    AACCTTCGCA TTGACAAACT GAAGCAGATG AGGAAGCCAT CAACCAAGGG CCTGAACCTG
    CCAGCCATCC CCGGAGCCGG TCTGCCCGGA GATGTGCCCA GGGTCTGCGT GCCGCCGCCG
    GCTGCGTCCC AGCGCCGCGT CGTGGAGCTG TCGGCTGCAG AGGTGGCCGT TCCGCCGAGC
    GAGCGCCGCA GCAGCGCCAC CAGCACCATC AGCTCAGCCT ACACCGTCAG CCGCCGCTCC
    TCCGCCGCCT CCCCGTACCT ACCGGGGCCG AACCTGCCCG GCGAGGGGGG GACGATGCCG
    GCACGCTCGG ATGGCTACGA CTGCATCTCT CCGGATGAGC TGCGGCGCTC CGGCAACGCC
    GATCCCTGCG GGGCGCTGCC GGGCGTGGGG AGCCTCACGG CGGCTCAGCA TTACCGTCTC
    AAAGCCAAAT ACGCCGCGGC CACGGGGGGG CCGCCCCCAA CTCCGCTGCC CGGCGTGGAG
    CCGCCGAACG TGGGCGGCCA CGGCGGTTTG CCCGGGGAGT GCCTCGAGCC GGCCGTGCCC
    TCCTTCTTCG CGAACGGTTT GCTGCGAAGG CACAGCTCCA ACGACCGCTA CGTGAGCAGC
    CTTCACCCTC ACCTGGCGCC TGGCAACGAC GTGAGGAGGG CCAGCGACCC GGCCCGGATT
    GCAGCCAACC CTCACTCTGT GCCCAAAGTG CAGCGTTTTA AAAGCGTGGG GAACGTGAAT
    GCGCCGGGAG GCGGCAGAGC TGCCCTGCAG CCCCTGGGTG CTTCCGACGG CAACCTGCAG
    CGCCGCGTCT TCTCCCCTCA CCCACCCAGC ATCAGTGAGA ATGTTTACCT GGAGAGCGTG
    AACCCGGACG GCCCCGGGCC TGGCGCGGGG TCTGGCTTGC TGGAAATGGA CCCGTTCTTG
    AACTACCCCG AGGAAAGCTT CCCGTGCCAG GGGGCAAACG TGCAGCTGCA GTGCGGGGGT
    CTGTATGGCA CCGCTCAGCG GACCCCGGGG GGCGGAATGC AGCTGAACCC GGGTGGGCAC
    AGGGAGATGG AGGAGGGATT GCTGCAGCCT GAGTTCTCCC TCCCGCAGTG CCAGATGAAC
    CAGCACTTCC ACGGCCTGCG TGGCGGCAGC GGGACCGTGC TGCCTCAATG GGAGGAGCCC
    CCCCAAAGCG ATCACCTGGA GATGGGCTCC GGGCAGCCAG CAGTGAGCGC CGTGCCTGAG
    ACGCACCGCC ACCGTCACAG CGCTCTGTAC CCACCGCCCA ACCACTGCGT GCAGCACCCC
    AAACCGATGA GCACGGGCCA CGATGGTGGC TTTTCAGATG GCCGTCGCTT GAACTGCCTG
    CAGATCAAAT CTGAGCAGCG GTACTCGGCT CCTGCCCCGG CCCTCACCCC GTGCCACGAT
    GCCAAAGCCA CGCGGCCGCC CGCGTTCGGC CGGGCTGTGA GTGGGTACCA GGCTGAGGAG
    CAGCCAGCCG GCGGCTACGC GGGCGTGCTG AGCCCACGGG GGAGGAGAGC CCACACCCCC
    ACTTCGCAGG CCAAAGAGGT GATGGTCCGC AGCTACGTGC AGGCGCAGCA GGCCCTGATG
    TGGGGAGAGC CCCCCAAGGG CGGTGAGGCC GGCGTGGGGC TGGGCGGTGA GTCTGGGCAG
    TACCAGGGCA CGCACGCAGC GCCGTACCTT GGCCCCAAGT ACTCTGGTTA CCAGCCCAAA
    GCCAACCACC TGCAGGGGCT GCTGGAGAAC CCCCACCTCC TGAGCCCCCC GTGCTTTAAC
    CCTGAGATGG TGCCACATCC CCCCAGCGGT GCCAAGCCAC CGGGCCACCA GAACAGCGTG
    GGCTATGGGG GCGACCTGGC AGATCCCATC CCCTCCTACG AGGCAGCGGG TGCCAACCCC
    CAGCGCGGGC TGCGCCTGCC CCCGGTCCAC CCTGCCCCCG AAGTGCCCGG TGATACACTG
    CTCTGCTACC CGGGCCAGGT GGGCAAAGGC AGCCACAAGC TTCAGGGTCC GCCTGCCGCA
    AGCTGTGGGG GTCCCGGGCA CTGCACTGGG AGCCTGGGGG GCAGCAAAGG CAGCTCTTGT
    TTCTACCCAG ACTCGGGGCA GCAGGCATTC AACAGCCTGG ACTCGCTGGA CCTGGAGAAC
    ACTCACCTGG ACTTTGCTGC CATCGTGGAG GATGCAGAGA GCCCCACGCT CCTGCCTGGC
    CCCCCCAGCC CCACTGGGGG TCTCCTGCTG CCAGCGCCCG GCGGTGCCAA CATGGCCGTG
    GGTGACATGA GCTCCATGCT GAGCACTTTG GCTGGGGAGA GCCACTTCCT CAACTCCCTC
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.