ankrd27 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ankrd27
Entrez Gene ID	100493046
Full Name	ankyrin repeat domain 27
General protein information	Preferred Names ankyrin repeat domain 27 Names ankyrin repeat domain-containing protein 27 ankyrin repeat domain 27 (VPS9 domain)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934381.5	XP_002934427.3	ankyrin repeat domain-containing protein 27
XM_004913553.4	XP_004913610.2	ankyrin repeat domain-containing protein 27
XM_002934381.4	XP_002934427.2	ankyrin repeat domain-containing protein 27
XM_004913553.3	XP_004913610.1	ankyrin repeat domain-containing protein 27

Pathways from BioSystems

Homologies

	LOC716232	XP_001106852.1
Rattus norvegicus (Norway rat)	Ankrd27	NP_001258193.1
Pan troglodytes (chimpanzee)	ANKRD27	XP_001151730.1
Bos taurus (cattle)	ANKRD27	NP_001096002.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003151	XP_001688409.2
Homo sapiens (human)	ANKRD27	NP_115515.2
Danio rerio (zebrafish)	wu:fc41e04	XP_005163257.1
Xenopus tropicalis (tropical clawed frog)	ankrd27	XP_004913610.1
Canis lupus familiaris (dog)	ANKRD27	XP_005616956.1
Mus musculus (house mouse)	Ankrd27	NP_663608.3
Gallus gallus (chicken)	ANKRD27	NP_001129150.1

Gene Ontology

The following ankrd27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ankrd27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa56929	XM_004913553.4 Latest version!	Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa56929	XM_002934381.5 Latest version!	Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa08794	XM_004913553.3 Latest version!	Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa08794	XM_002934381.4 Latest version!	Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ankrd27 ( XM_002934381.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934427.3 Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa56929
Clone ID Related Accession (Same CDS sequence)	XM_002934381.5 , XM_004913553.4
Accession Version	XM_002934381.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat domain-containing protein 27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934381.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATGT ATGATGAAGA CATTTTGAAC AATCCCTTTT ATTTAGCTAT TCAGAAACAG 
AGACCTGATT TAATTGATAA AGTTGCAGAG GCACATGGAA TTGTCCTGGT TCCATGCAAG 
GGCAGTGTTT CCAACATCTT GAATTCTACA TGTCCTTTTG ATTCGTATAT TTTAAAGCCC 
ACAGAAGGGG GGTTTGAGAC AGAAAATGGA AAGGAGATCT TTATTCAAGG AAACCTGGTT 
AAACTGGGTT CAGGTTTTGC CTACCACCTC ACCATTCCAA TTCTTTTTGA GGAGACTTTC 
TACAATGAAA AAGAGGAAAG CTATAACATT TTGTGTATTG CGCGTTCATT AGAAAAAGAG 
GAAAATTCAG AAGAACACTC AACGCCCTCA AAATTTTGCA CTCTGAAAAA TATAGAAGAT 
GTTAAAGAAT TTTTAGGAAA GCACGCTGAG AAGTTTGACA AAAGTATTGC ATCTTTCCGA 
AGCACATTTA AAGTTGAAAG GAAAAGCCTT CGCCATTACA TTGACTCTGT AAATGCCCTG 
TACACAAAAT GTCTTCAGCA TTTACTGCGA GACTCACACT TGAGAGCAAT AGCAAAGCAA 
GAAACGCAAA TGAGCTTGAT GAAACAGGCA GTGGAGATGT ATGTACATCA TGGGATTTAT 
GATCTAATTT TCAAACAAGT TGGAACAATT GAAGCAAGTG AGGATGCAGC ATTTAACAAG 
ATTACAAGAA GCCTTCAGGA TCTCCAGCAA AAGGATATTG GTGTAAAGAC GGAGTTCAGC 
CTTAACATAC CCAGCGCAAA GCGTGTTCTA TGCCAGTTGA ACAAATGCAC GTCCCCCCAG 
CAAAAACTTT TATGCTTGCG GAAGGTGGTA CAGACTATCA TGCATTCTTC TAGTCAAAGG 
GTGAACATGG AGACAATGTG TGCTGATGAC CTTCTGTCGG TGTTGCTGTA CTTGTTAGTA 
AAAACAGAGA TACCCAACTG GATGGCAAAT TTAAGCTACA TAAAGAATTT TCGTTTTTGC 
AACTCAACAA AAGATGAGTT GGGATACTGC CTAACCTCTG TGGAAGCAGC AATTGAGTTT 
ATCCGTCAAG GAAATTTAAC AGAAACAGCA ACAGCAGAAA CTGAAGGAAA TGATAAGCCA 
TTTTTATGGC AAAGAATGAA CTTAATGTAC AGAGCCTCTT CTACTCCTAT TGATTGCTTA 
TTCAAGCACA TTGCTGCAGG AAATCAAAAA GAAGTAGAGA AGTTACTGAG CCAAGGTGAC 
AGTGATGAAG AAATTGTGCA AAAAATGTGC CACCCACTCT GCTCATGTGA CAACTGTGAA 
AAGCTGGTCT CTGGGAAACT AAATGATACT TCAATTGTTA CCCCATTCTC AAGAGATGAT 
CGAGGTTATA CTCCCCTCCA TATCGCAGCA CTTTATGGTC AGGCTCAGTG TGTTGACTTA 
CTGATATCTA AAGGAGCTGT AGTAAATGCC ACAGACTACC TTGGTTCAAC ACCACTTCAT 
CTTTCGTGCC AGAAAGGCCA CCAGAAGATT GCACTTCTGC TGTTGCATTT TAAGGCATCC 
AGTGACATAC AAGACAATAA CGGCAACACT CCACTACATT TAGCTTGTAC ATATGGACAT 
GAAGATTGTG TGAAAGCTTT AGTCTATTAT GATTTAAACT CCTGTAAAAT TGATACTGTT 
AATGAGAAGG GAGATACCCC TCTCCACATA GCTGCTCGAT GGGGCTACCA AGGAATTATT 
GAGGTCCTGT TAGAGAATGG AGCTAATACT GACATACAGA ACAAGAGAAA GGAAACTCCT 
ATGCAGTGTG CTTTAAATGA AAAGATCCTC ACACTCATGG AAACAGTCTA TGTTACAAAT 
GACCGGAGAC AGAGTATCTC CGAGTCATTG AGCAAATCCC TTCAGTCTGA AGATGCAATC 
AGTGAATCCT CACAATCCAG TTTGTCGTCT GCATCACCTG ACACCAGACC AGAAGATGTG 
AAAAGCAAAT ACAAAGAGGT TGAAAAACTT CTTAGAGCTG TTTCTGATGG TGATCTGGAG 
ATGGTCCGTT ACCTCTTGGA ATGGGTGGAT GATGACTTTG AAGAAGATGA TTCTCCAGTT 
GGGAACAGAG AATTTTGCCA TCCATTATGC CAGTGTACCA AATGTGGTCC TGCTCAGAAG 
AGACTAAGCA AGATTCCACA GAATGGGCTG AATGTCAATG TAACGAGCAG AGATGGCTTC 
ACTCCTCTGC ACATCTCTGC ACTGCAAGGG CATCTTGTTT TAGTGTGTCT CCTTTTAAAG 
CACGGTGCCA GTGTTGATGT AAAGAATGGT AATCGTGCAT TACCTTTGCA CTTGGCCTGC 
CATAAAGGTC ACCTTGAGGT GGTGAAGACT CTAATGGAAT ATAGTACAGG GAAGAATAAA 
AAAGACATGA ATGGAAATAC GCCCTTATTA TACGCATGCA TGGGGGGCCA TCTGGAAATT 
GCATTATTGT TGTTAGAGCA TGGCGCATCT GTTAATATTC GCAATGTAAA AGGGAACACC 
GCACTTCATG AGGCAGTGAG GAGGAATCAC GAGGGTTTAG TGCAGCAGTT GCTCATATAT 
GGTGCACTAG TGGATGCAAG GAACAAACGC CAATATACGC CTTTGGATTA TGCTAAAGAG 
AATTCTAGGA TTAAACAGCT TTTGCTGACA AGCGACAGGA TTGCTGGAGA TGGATTTACT 
ACAGATGAAC ACACCATCCG CATCAGGAAA AAATCAAGCA CCTCCAGTCC TAGAGGAGAA 
GAGGGAGGAC TTACACCAAT GCAACACGAG GTGGCTAAAT CAACAGGTTT GAAAAAAATA 
ATACGGAGAG ACAAAAGTAT GCCTAATATT GATGAGGACC TACTGAATAA GTTATCAGTA 
AAGCAGATAG AGGGCGGTAA CTTAAAACAA ATCAGCAATG ATAAAATGAG CAGAAGCAGC 
GCTGAAGTTC AAGAAGCCCT GGAAGGTGAT GACCTGCCGT CCCCACTTAA AACAAAACTT 
TTTCTGAGAA GACATAGCCT GAATGTTGCT TGTTTAGCAG ATACACAAGA GGACTCTAGT 
GAGGCCACCG ATACCTTAAA TGGGACAGAT GCAAGTTCCC CCTTGTCTGA AACTTGCGAC 
GTGGATTTAG AACATGAAGA TTGCCTAAAC AACCAGGAGT TGTCTGAAAT GAATTGGCAA 
AAGGAGCCCC TTTCTGCATG GACTGCTGTT AAGGAACTAC TAAAAACAGA TGAGTTTGAC 
TGTTCTGGAA AAAAAGACTG TTCTTGTGTG GCTTGTACTT TGGCATTAGA ATCTTCTAGG 
TCTGATCAGT CCTGCTGTGT GAGCTCCGAA CAGGACGGTT TTGATACAGT CCCTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934427.3
CDS	288..3647
Translation	MAMYDEDILNNPFYLAIQKQRPDLIDKVAEAHGIVLVPCKGSVSNILNSTCPFDSYILKPTEGGFETENGKEIFIQGNLVKLGSGFAYHLTIPILFEETFYNEKEESYNILCIARSLEKEENSEEHSTPSKFCTLKNIEDVKEFLGKHAEKFDKSIASFRSTFKVERKSLRHYIDSVNALYTKCLQHLLRDSHLRAIAKQETQMSLMKQAVEMYVHHGIYDLIFKQVGTIEASEDAAFNKITRSLQDLQQKDIGVKTEFSLNIPSAKRVLCQLNKCTSPQQKLLCLRKVVQTIMHSSSQRVNMETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFCNSTKDELGYCLTSVEAAIEFIRQGNLTETATAETEGNDKPFLWQRMNLMYRASSTPIDCLFKHIAAGNQKEVEKLLSQGDSDEEIVQKMCHPLCSCDNCEKLVSGKLNDTSIVTPFSRDDRGYTPLHIAALYGQAQCVDLLISKGAVVNATDYLGSTPLHLSCQKGHQKIALLLLHFKASSDIQDNNGNTPLHLACTYGHEDCVKALVYYDLNSCKIDTVNEKGDTPLHIAARWGYQGIIEVLLENGANTDIQNKRKETPMQCALNEKILTLMETVYVTNDRRQSISESLSKSLQSEDAISESSQSSLSSASPDTRPEDVKSKYKEVEKLLRAVSDGDLEMVRYLLEWVDDDFEEDDSPVGNREFCHPLCQCTKCGPAQKRLSKIPQNGLNVNVTSRDGFTPLHISALQGHLVLVCLLLKHGASVDVKNGNRALPLHLACHKGHLEVVKTLMEYSTGKNKKDMNGNTPLLYACMGGHLEIALLLLEHGASVNIRNVKGNTALHEAVRRNHEGLVQQLLIYGALVDARNKRQYTPLDYAKENSRIKQLLLTSDRIAGDGFTTDEHTIRIRKKSSTSSPRGEEGGLTPMQHEVAKSTGLKKIIRRDKSMPNIDEDLLNKLSVKQIEGGNLKQISNDKMSRSSAEVQEALEGDDLPSPLKTKLFLRRHSLNVACLADTQEDSSEATDTLNGTDASSPLSETCDVDLEHEDCLNNQELSEMNWQKEPLSAWTAVKELLKTDEFDCSGKKDCSCVACTLALESSRSDQSCCVSSEQDGFDTVPL

Target ORF information:

RefSeq Version	XM_002934381.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934381.5

ORF Insert Sequence:

ATGGCTATGT ATGATGAAGA CATTTTGAAC AATCCCTTTT ATTTAGCTAT TCAGAAACAG 
AGACCTGATT TAATTGATAA AGTTGCAGAG GCACATGGAA TTGTCCTGGT TCCATGCAAG 
GGCAGTGTTT CCAACATCTT GAATTCTACA TGTCCTTTTG ATTCGTATAT TTTAAAGCCC 
ACAGAAGGGG GGTTTGAGAC AGAAAATGGA AAGGAGATCT TTATTCAAGG AAACCTGGTT 
AAACTGGGTT CAGGTTTTGC CTACCACCTC ACCATTCCAA TTCTTTTTGA GGAGACTTTC 
TACAATGAAA AAGAGGAAAG CTATAACATT TTGTGTATTG CGCGTTCATT AGAAAAAGAG 
GAAAATTCAG AAGAACACTC AACGCCCTCA AAATTTTGCA CTCTGAAAAA TATAGAAGAT 
GTTAAAGAAT TTTTAGGAAA GCACGCTGAG AAGTTTGACA AAAGTATTGC ATCTTTCCGA 
AGCACATTTA AAGTTGAAAG GAAAAGCCTT CGCCATTACA TTGACTCTGT AAATGCCCTG 
TACACAAAAT GTCTTCAGCA TTTACTGCGA GACTCACACT TGAGAGCAAT AGCAAAGCAA 
GAAACGCAAA TGAGCTTGAT GAAACAGGCA GTGGAGATGT ATGTACATCA TGGGATTTAT 
GATCTAATTT TCAAACAAGT TGGAACAATT GAAGCAAGTG AGGATGCAGC ATTTAACAAG 
ATTACAAGAA GCCTTCAGGA TCTCCAGCAA AAGGATATTG GTGTAAAGAC GGAGTTCAGC 
CTTAACATAC CCAGCGCAAA GCGTGTTCTA TGCCAGTTGA ACAAATGCAC GTCCCCCCAG 
CAAAAACTTT TATGCTTGCG GAAGGTGGTA CAGACTATCA TGCATTCTTC TAGTCAAAGG 
GTGAACATGG AGACAATGTG TGCTGATGAC CTTCTGTCGG TGTTGCTGTA CTTGTTAGTA 
AAAACAGAGA TACCCAACTG GATGGCAAAT TTAAGCTACA TAAAGAATTT TCGTTTTTGC 
AACTCAACAA AAGATGAGTT GGGATACTGC CTAACCTCTG TGGAAGCAGC AATTGAGTTT 
ATCCGTCAAG GAAATTTAAC AGAAACAGCA ACAGCAGAAA CTGAAGGAAA TGATAAGCCA 
TTTTTATGGC AAAGAATGAA CTTAATGTAC AGAGCCTCTT CTACTCCTAT TGATTGCTTA 
TTCAAGCACA TTGCTGCAGG AAATCAAAAA GAAGTAGAGA AGTTACTGAG CCAAGGTGAC 
AGTGATGAAG AAATTGTGCA AAAAATGTGC CACCCACTCT GCTCATGTGA CAACTGTGAA 
AAGCTGGTCT CTGGGAAACT AAATGATACT TCAATTGTTA CCCCATTCTC AAGAGATGAT 
CGAGGTTATA CTCCCCTCCA TATCGCAGCA CTTTATGGTC AGGCTCAGTG TGTTGACTTA 
CTGATATCTA AAGGAGCTGT AGTAAATGCC ACAGACTACC TTGGTTCAAC ACCACTTCAT 
CTTTCGTGCC AGAAAGGCCA CCAGAAGATT GCACTTCTGC TGTTGCATTT TAAGGCATCC 
AGTGACATAC AAGACAATAA CGGCAACACT CCACTACATT TAGCTTGTAC ATATGGACAT 
GAAGATTGTG TGAAAGCTTT AGTCTATTAT GATTTAAACT CCTGTAAAAT TGATACTGTT 
AATGAGAAGG GAGATACCCC TCTCCACATA GCTGCTCGAT GGGGCTACCA AGGAATTATT 
GAGGTCCTGT TAGAGAATGG AGCTAATACT GACATACAGA ACAAGAGAAA GGAAACTCCT 
ATGCAGTGTG CTTTAAATGA AAAGATCCTC ACACTCATGG AAACAGTCTA TGTTACAAAT 
GACCGGAGAC AGAGTATCTC CGAGTCATTG AGCAAATCCC TTCAGTCTGA AGATGCAATC 
AGTGAATCCT CACAATCCAG TTTGTCGTCT GCATCACCTG ACACCAGACC AGAAGATGTG 
AAAAGCAAAT ACAAAGAGGT TGAAAAACTT CTTAGAGCTG TTTCTGATGG TGATCTGGAG 
ATGGTCCGTT ACCTCTTGGA ATGGGTGGAT GATGACTTTG AAGAAGATGA TTCTCCAGTT 
GGGAACAGAG AATTTTGCCA TCCATTATGC CAGTGTACCA AATGTGGTCC TGCTCAGAAG 
AGACTAAGCA AGATTCCACA GAATGGGCTG AATGTCAATG TAACGAGCAG AGATGGCTTC 
ACTCCTCTGC ACATCTCTGC ACTGCAAGGG CATCTTGTTT TAGTGTGTCT CCTTTTAAAG 
CACGGTGCCA GTGTTGATGT AAAGAATGGT AATCGTGCAT TACCTTTGCA CTTGGCCTGC 
CATAAAGGTC ACCTTGAGGT GGTGAAGACT CTAATGGAAT ATAGTACAGG GAAGAATAAA 
AAAGACATGA ATGGAAATAC GCCCTTATTA TACGCATGCA TGGGGGGCCA TCTGGAAATT 
GCATTATTGT TGTTAGAGCA TGGCGCATCT GTTAATATTC GCAATGTAAA AGGGAACACC 
GCACTTCATG AGGCAGTGAG GAGGAATCAC GAGGGTTTAG TGCAGCAGTT GCTCATATAT 
GGTGCACTAG TGGATGCAAG GAACAAACGC CAATATACGC CTTTGGATTA TGCTAAAGAG 
AATTCTAGGA TTAAACAGCT TTTGCTGACA AGCGACAGGA TTGCTGGAGA TGGATTTACT 
ACAGATGAAC ACACCATCCG CATCAGGAAA AAATCAAGCA CCTCCAGTCC TAGAGGAGAA 
GAGGGAGGAC TTACACCAAT GCAACACGAG GTGGCTAAAT CAACAGGTTT GAAAAAAATA 
ATACGGAGAG ACAAAAGTAT GCCTAATATT GATGAGGACC TACTGAATAA GTTATCAGTA 
AAGCAGATAG AGGGCGGTAA CTTAAAACAA ATCAGCAATG ATAAAATGAG CAGAAGCAGC 
GCTGAAGTTC AAGAAGCCCT GGAAGGTGAT GACCTGCCGT CCCCACTTAA AACAAAACTT 
TTTCTGAGAA GACATAGCCT GAATGTTGCT TGTTTAGCAG ATACACAAGA GGACTCTAGT 
GAGGCCACCG ATACCTTAAA TGGGACAGAT GCAAGTTCCC CCTTGTCTGA AACTTGCGAC 
GTGGATTTAG AACATGAAGA TTGCCTAAAC AACCAGGAGT TGTCTGAAAT GAATTGGCAA 
AAGGAGCCCC TTTCTGCATG GACTGCTGTT AAGGAACTAC TAAAAACAGA TGAGTTTGAC 
TGTTCTGGAA AAAAAGACTG TTCTTGTGTG GCTTGTACTT TGGCATTAGA ATCTTCTAGG 
TCTGATCAGT CCTGCTGTGT GAGCTCCGAA CAGGACGGTT TTGATACAGT CCCTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankrd27 ( XM_004913553.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913610.2 Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa56929
Clone ID Related Accession (Same CDS sequence)	XM_002934381.5 , XM_004913553.4
Accession Version	XM_004913553.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat domain-containing protein 27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913553.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATGT ATGATGAAGA CATTTTGAAC AATCCCTTTT ATTTAGCTAT TCAGAAACAG 
AGACCTGATT TAATTGATAA AGTTGCAGAG GCACATGGAA TTGTCCTGGT TCCATGCAAG 
GGCAGTGTTT CCAACATCTT GAATTCTACA TGTCCTTTTG ATTCGTATAT TTTAAAGCCC 
ACAGAAGGGG GGTTTGAGAC AGAAAATGGA AAGGAGATCT TTATTCAAGG AAACCTGGTT 
AAACTGGGTT CAGGTTTTGC CTACCACCTC ACCATTCCAA TTCTTTTTGA GGAGACTTTC 
TACAATGAAA AAGAGGAAAG CTATAACATT TTGTGTATTG CGCGTTCATT AGAAAAAGAG 
GAAAATTCAG AAGAACACTC AACGCCCTCA AAATTTTGCA CTCTGAAAAA TATAGAAGAT 
GTTAAAGAAT TTTTAGGAAA GCACGCTGAG AAGTTTGACA AAAGTATTGC ATCTTTCCGA 
AGCACATTTA AAGTTGAAAG GAAAAGCCTT CGCCATTACA TTGACTCTGT AAATGCCCTG 
TACACAAAAT GTCTTCAGCA TTTACTGCGA GACTCACACT TGAGAGCAAT AGCAAAGCAA 
GAAACGCAAA TGAGCTTGAT GAAACAGGCA GTGGAGATGT ATGTACATCA TGGGATTTAT 
GATCTAATTT TCAAACAAGT TGGAACAATT GAAGCAAGTG AGGATGCAGC ATTTAACAAG 
ATTACAAGAA GCCTTCAGGA TCTCCAGCAA AAGGATATTG GTGTAAAGAC GGAGTTCAGC 
CTTAACATAC CCAGCGCAAA GCGTGTTCTA TGCCAGTTGA ACAAATGCAC GTCCCCCCAG 
CAAAAACTTT TATGCTTGCG GAAGGTGGTA CAGACTATCA TGCATTCTTC TAGTCAAAGG 
GTGAACATGG AGACAATGTG TGCTGATGAC CTTCTGTCGG TGTTGCTGTA CTTGTTAGTA 
AAAACAGAGA TACCCAACTG GATGGCAAAT TTAAGCTACA TAAAGAATTT TCGTTTTTGC 
AACTCAACAA AAGATGAGTT GGGATACTGC CTAACCTCTG TGGAAGCAGC AATTGAGTTT 
ATCCGTCAAG GAAATTTAAC AGAAACAGCA ACAGCAGAAA CTGAAGGAAA TGATAAGCCA 
TTTTTATGGC AAAGAATGAA CTTAATGTAC AGAGCCTCTT CTACTCCTAT TGATTGCTTA 
TTCAAGCACA TTGCTGCAGG AAATCAAAAA GAAGTAGAGA AGTTACTGAG CCAAGGTGAC 
AGTGATGAAG AAATTGTGCA AAAAATGTGC CACCCACTCT GCTCATGTGA CAACTGTGAA 
AAGCTGGTCT CTGGGAAACT AAATGATACT TCAATTGTTA CCCCATTCTC AAGAGATGAT 
CGAGGTTATA CTCCCCTCCA TATCGCAGCA CTTTATGGTC AGGCTCAGTG TGTTGACTTA 
CTGATATCTA AAGGAGCTGT AGTAAATGCC ACAGACTACC TTGGTTCAAC ACCACTTCAT 
CTTTCGTGCC AGAAAGGCCA CCAGAAGATT GCACTTCTGC TGTTGCATTT TAAGGCATCC 
AGTGACATAC AAGACAATAA CGGCAACACT CCACTACATT TAGCTTGTAC ATATGGACAT 
GAAGATTGTG TGAAAGCTTT AGTCTATTAT GATTTAAACT CCTGTAAAAT TGATACTGTT 
AATGAGAAGG GAGATACCCC TCTCCACATA GCTGCTCGAT GGGGCTACCA AGGAATTATT 
GAGGTCCTGT TAGAGAATGG AGCTAATACT GACATACAGA ACAAGAGAAA GGAAACTCCT 
ATGCAGTGTG CTTTAAATGA AAAGATCCTC ACACTCATGG AAACAGTCTA TGTTACAAAT 
GACCGGAGAC AGAGTATCTC CGAGTCATTG AGCAAATCCC TTCAGTCTGA AGATGCAATC 
AGTGAATCCT CACAATCCAG TTTGTCGTCT GCATCACCTG ACACCAGACC AGAAGATGTG 
AAAAGCAAAT ACAAAGAGGT TGAAAAACTT CTTAGAGCTG TTTCTGATGG TGATCTGGAG 
ATGGTCCGTT ACCTCTTGGA ATGGGTGGAT GATGACTTTG AAGAAGATGA TTCTCCAGTT 
GGGAACAGAG AATTTTGCCA TCCATTATGC CAGTGTACCA AATGTGGTCC TGCTCAGAAG 
AGACTAAGCA AGATTCCACA GAATGGGCTG AATGTCAATG TAACGAGCAG AGATGGCTTC 
ACTCCTCTGC ACATCTCTGC ACTGCAAGGG CATCTTGTTT TAGTGTGTCT CCTTTTAAAG 
CACGGTGCCA GTGTTGATGT AAAGAATGGT AATCGTGCAT TACCTTTGCA CTTGGCCTGC 
CATAAAGGTC ACCTTGAGGT GGTGAAGACT CTAATGGAAT ATAGTACAGG GAAGAATAAA 
AAAGACATGA ATGGAAATAC GCCCTTATTA TACGCATGCA TGGGGGGCCA TCTGGAAATT 
GCATTATTGT TGTTAGAGCA TGGCGCATCT GTTAATATTC GCAATGTAAA AGGGAACACC 
GCACTTCATG AGGCAGTGAG GAGGAATCAC GAGGGTTTAG TGCAGCAGTT GCTCATATAT 
GGTGCACTAG TGGATGCAAG GAACAAACGC CAATATACGC CTTTGGATTA TGCTAAAGAG 
AATTCTAGGA TTAAACAGCT TTTGCTGACA AGCGACAGGA TTGCTGGAGA TGGATTTACT 
ACAGATGAAC ACACCATCCG CATCAGGAAA AAATCAAGCA CCTCCAGTCC TAGAGGAGAA 
GAGGGAGGAC TTACACCAAT GCAACACGAG GTGGCTAAAT CAACAGGTTT GAAAAAAATA 
ATACGGAGAG ACAAAAGTAT GCCTAATATT GATGAGGACC TACTGAATAA GTTATCAGTA 
AAGCAGATAG AGGGCGGTAA CTTAAAACAA ATCAGCAATG ATAAAATGAG CAGAAGCAGC 
GCTGAAGTTC AAGAAGCCCT GGAAGGTGAT GACCTGCCGT CCCCACTTAA AACAAAACTT 
TTTCTGAGAA GACATAGCCT GAATGTTGCT TGTTTAGCAG ATACACAAGA GGACTCTAGT 
GAGGCCACCG ATACCTTAAA TGGGACAGAT GCAAGTTCCC CCTTGTCTGA AACTTGCGAC 
GTGGATTTAG AACATGAAGA TTGCCTAAAC AACCAGGAGT TGTCTGAAAT GAATTGGCAA 
AAGGAGCCCC TTTCTGCATG GACTGCTGTT AAGGAACTAC TAAAAACAGA TGAGTTTGAC 
TGTTCTGGAA AAAAAGACTG TTCTTGTGTG GCTTGTACTT TGGCATTAGA ATCTTCTAGG 
TCTGATCAGT CCTGCTGTGT GAGCTCCGAA CAGGACGGTT TTGATACAGT CCCTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913610.2
CDS	481..3840
Translation	MAMYDEDILNNPFYLAIQKQRPDLIDKVAEAHGIVLVPCKGSVSNILNSTCPFDSYILKPTEGGFETENGKEIFIQGNLVKLGSGFAYHLTIPILFEETFYNEKEESYNILCIARSLEKEENSEEHSTPSKFCTLKNIEDVKEFLGKHAEKFDKSIASFRSTFKVERKSLRHYIDSVNALYTKCLQHLLRDSHLRAIAKQETQMSLMKQAVEMYVHHGIYDLIFKQVGTIEASEDAAFNKITRSLQDLQQKDIGVKTEFSLNIPSAKRVLCQLNKCTSPQQKLLCLRKVVQTIMHSSSQRVNMETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFCNSTKDELGYCLTSVEAAIEFIRQGNLTETATAETEGNDKPFLWQRMNLMYRASSTPIDCLFKHIAAGNQKEVEKLLSQGDSDEEIVQKMCHPLCSCDNCEKLVSGKLNDTSIVTPFSRDDRGYTPLHIAALYGQAQCVDLLISKGAVVNATDYLGSTPLHLSCQKGHQKIALLLLHFKASSDIQDNNGNTPLHLACTYGHEDCVKALVYYDLNSCKIDTVNEKGDTPLHIAARWGYQGIIEVLLENGANTDIQNKRKETPMQCALNEKILTLMETVYVTNDRRQSISESLSKSLQSEDAISESSQSSLSSASPDTRPEDVKSKYKEVEKLLRAVSDGDLEMVRYLLEWVDDDFEEDDSPVGNREFCHPLCQCTKCGPAQKRLSKIPQNGLNVNVTSRDGFTPLHISALQGHLVLVCLLLKHGASVDVKNGNRALPLHLACHKGHLEVVKTLMEYSTGKNKKDMNGNTPLLYACMGGHLEIALLLLEHGASVNIRNVKGNTALHEAVRRNHEGLVQQLLIYGALVDARNKRQYTPLDYAKENSRIKQLLLTSDRIAGDGFTTDEHTIRIRKKSSTSSPRGEEGGLTPMQHEVAKSTGLKKIIRRDKSMPNIDEDLLNKLSVKQIEGGNLKQISNDKMSRSSAEVQEALEGDDLPSPLKTKLFLRRHSLNVACLADTQEDSSEATDTLNGTDASSPLSETCDVDLEHEDCLNNQELSEMNWQKEPLSAWTAVKELLKTDEFDCSGKKDCSCVACTLALESSRSDQSCCVSSEQDGFDTVPL

Target ORF information:

RefSeq Version	XM_004913553.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913553.4

ORF Insert Sequence:

ATGGCTATGT ATGATGAAGA CATTTTGAAC AATCCCTTTT ATTTAGCTAT TCAGAAACAG 
AGACCTGATT TAATTGATAA AGTTGCAGAG GCACATGGAA TTGTCCTGGT TCCATGCAAG 
GGCAGTGTTT CCAACATCTT GAATTCTACA TGTCCTTTTG ATTCGTATAT TTTAAAGCCC 
ACAGAAGGGG GGTTTGAGAC AGAAAATGGA AAGGAGATCT TTATTCAAGG AAACCTGGTT 
AAACTGGGTT CAGGTTTTGC CTACCACCTC ACCATTCCAA TTCTTTTTGA GGAGACTTTC 
TACAATGAAA AAGAGGAAAG CTATAACATT TTGTGTATTG CGCGTTCATT AGAAAAAGAG 
GAAAATTCAG AAGAACACTC AACGCCCTCA AAATTTTGCA CTCTGAAAAA TATAGAAGAT 
GTTAAAGAAT TTTTAGGAAA GCACGCTGAG AAGTTTGACA AAAGTATTGC ATCTTTCCGA 
AGCACATTTA AAGTTGAAAG GAAAAGCCTT CGCCATTACA TTGACTCTGT AAATGCCCTG 
TACACAAAAT GTCTTCAGCA TTTACTGCGA GACTCACACT TGAGAGCAAT AGCAAAGCAA 
GAAACGCAAA TGAGCTTGAT GAAACAGGCA GTGGAGATGT ATGTACATCA TGGGATTTAT 
GATCTAATTT TCAAACAAGT TGGAACAATT GAAGCAAGTG AGGATGCAGC ATTTAACAAG 
ATTACAAGAA GCCTTCAGGA TCTCCAGCAA AAGGATATTG GTGTAAAGAC GGAGTTCAGC 
CTTAACATAC CCAGCGCAAA GCGTGTTCTA TGCCAGTTGA ACAAATGCAC GTCCCCCCAG 
CAAAAACTTT TATGCTTGCG GAAGGTGGTA CAGACTATCA TGCATTCTTC TAGTCAAAGG 
GTGAACATGG AGACAATGTG TGCTGATGAC CTTCTGTCGG TGTTGCTGTA CTTGTTAGTA 
AAAACAGAGA TACCCAACTG GATGGCAAAT TTAAGCTACA TAAAGAATTT TCGTTTTTGC 
AACTCAACAA AAGATGAGTT GGGATACTGC CTAACCTCTG TGGAAGCAGC AATTGAGTTT 
ATCCGTCAAG GAAATTTAAC AGAAACAGCA ACAGCAGAAA CTGAAGGAAA TGATAAGCCA 
TTTTTATGGC AAAGAATGAA CTTAATGTAC AGAGCCTCTT CTACTCCTAT TGATTGCTTA 
TTCAAGCACA TTGCTGCAGG AAATCAAAAA GAAGTAGAGA AGTTACTGAG CCAAGGTGAC 
AGTGATGAAG AAATTGTGCA AAAAATGTGC CACCCACTCT GCTCATGTGA CAACTGTGAA 
AAGCTGGTCT CTGGGAAACT AAATGATACT TCAATTGTTA CCCCATTCTC AAGAGATGAT 
CGAGGTTATA CTCCCCTCCA TATCGCAGCA CTTTATGGTC AGGCTCAGTG TGTTGACTTA 
CTGATATCTA AAGGAGCTGT AGTAAATGCC ACAGACTACC TTGGTTCAAC ACCACTTCAT 
CTTTCGTGCC AGAAAGGCCA CCAGAAGATT GCACTTCTGC TGTTGCATTT TAAGGCATCC 
AGTGACATAC AAGACAATAA CGGCAACACT CCACTACATT TAGCTTGTAC ATATGGACAT 
GAAGATTGTG TGAAAGCTTT AGTCTATTAT GATTTAAACT CCTGTAAAAT TGATACTGTT 
AATGAGAAGG GAGATACCCC TCTCCACATA GCTGCTCGAT GGGGCTACCA AGGAATTATT 
GAGGTCCTGT TAGAGAATGG AGCTAATACT GACATACAGA ACAAGAGAAA GGAAACTCCT 
ATGCAGTGTG CTTTAAATGA AAAGATCCTC ACACTCATGG AAACAGTCTA TGTTACAAAT 
GACCGGAGAC AGAGTATCTC CGAGTCATTG AGCAAATCCC TTCAGTCTGA AGATGCAATC 
AGTGAATCCT CACAATCCAG TTTGTCGTCT GCATCACCTG ACACCAGACC AGAAGATGTG 
AAAAGCAAAT ACAAAGAGGT TGAAAAACTT CTTAGAGCTG TTTCTGATGG TGATCTGGAG 
ATGGTCCGTT ACCTCTTGGA ATGGGTGGAT GATGACTTTG AAGAAGATGA TTCTCCAGTT 
GGGAACAGAG AATTTTGCCA TCCATTATGC CAGTGTACCA AATGTGGTCC TGCTCAGAAG 
AGACTAAGCA AGATTCCACA GAATGGGCTG AATGTCAATG TAACGAGCAG AGATGGCTTC 
ACTCCTCTGC ACATCTCTGC ACTGCAAGGG CATCTTGTTT TAGTGTGTCT CCTTTTAAAG 
CACGGTGCCA GTGTTGATGT AAAGAATGGT AATCGTGCAT TACCTTTGCA CTTGGCCTGC 
CATAAAGGTC ACCTTGAGGT GGTGAAGACT CTAATGGAAT ATAGTACAGG GAAGAATAAA 
AAAGACATGA ATGGAAATAC GCCCTTATTA TACGCATGCA TGGGGGGCCA TCTGGAAATT 
GCATTATTGT TGTTAGAGCA TGGCGCATCT GTTAATATTC GCAATGTAAA AGGGAACACC 
GCACTTCATG AGGCAGTGAG GAGGAATCAC GAGGGTTTAG TGCAGCAGTT GCTCATATAT 
GGTGCACTAG TGGATGCAAG GAACAAACGC CAATATACGC CTTTGGATTA TGCTAAAGAG 
AATTCTAGGA TTAAACAGCT TTTGCTGACA AGCGACAGGA TTGCTGGAGA TGGATTTACT 
ACAGATGAAC ACACCATCCG CATCAGGAAA AAATCAAGCA CCTCCAGTCC TAGAGGAGAA 
GAGGGAGGAC TTACACCAAT GCAACACGAG GTGGCTAAAT CAACAGGTTT GAAAAAAATA 
ATACGGAGAG ACAAAAGTAT GCCTAATATT GATGAGGACC TACTGAATAA GTTATCAGTA 
AAGCAGATAG AGGGCGGTAA CTTAAAACAA ATCAGCAATG ATAAAATGAG CAGAAGCAGC 
GCTGAAGTTC AAGAAGCCCT GGAAGGTGAT GACCTGCCGT CCCCACTTAA AACAAAACTT 
TTTCTGAGAA GACATAGCCT GAATGTTGCT TGTTTAGCAG ATACACAAGA GGACTCTAGT 
GAGGCCACCG ATACCTTAAA TGGGACAGAT GCAAGTTCCC CCTTGTCTGA AACTTGCGAC 
GTGGATTTAG AACATGAAGA TTGCCTAAAC AACCAGGAGT TGTCTGAAAT GAATTGGCAA 
AAGGAGCCCC TTTCTGCATG GACTGCTGTT AAGGAACTAC TAAAAACAGA TGAGTTTGAC 
TGTTCTGGAA AAAAAGACTG TTCTTGTGTG GCTTGTACTT TGGCATTAGA ATCTTCTAGG 
TCTGATCAGT CCTGCTGTGT GAGCTCCGAA CAGGACGGTT TTGATACAGT CCCTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankrd27 ( XM_002934381.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934427.2 Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa08794
Clone ID Related Accession (Same CDS sequence)	XM_002934381.4 , XM_004913553.3
Accession Version	XM_002934381.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat domain-containing protein 27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934381.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATGT ATGATGAAGA CATTTTGAAC AATCCCTTTT ATTTAGCTAT TCAGAAACAG 
AGACCTGATT TAATTGATAA AGTTGCAGAG GCACATGGAA TTGTCCTGGT TCCATGCAAG 
GGCAGTGTTT CCAACATCTT GAATTCTACA TGTCCTTTTG ATTCGTATAT TTTAAAGCCC 
ACAGAAGGGG GGTTTGAGAC AGAAAATGGA AAGGAGATCT TTATTCAAGG AAACCTGGTT 
AAACTGGGTT CAGGTTTTGC CTACCACCTC ACCATTCCAA TTCTTTTTGA GGAGACTTTC 
TACAATGAAA AAGAGGAAAG CTATAACATT TTGTGTATTG CACGTTCATT AGAAAAAGAG 
GAAAATTCAG AAGAACACTC AACGCCCTCA AAATTTTGCA CTCTGAAAAA TATAGAAGAT 
GTTAAAGAAT TTTTAGGAAA GCACGCTGAG AAGTTTGACA AAAGTATTGC ATCTTTCCGA 
AGCACATTTA AAGTTGAAAG GAAAAGCCTT CGCCATTACA TTGACTCTGT GAATGCCCTG 
TACACAAAAT GTCTTCAGCA TTTACTGCGA GACTCACACT TGAGAGCAAT AGCAAAGCAA 
GAAACGCAAA TGAGCTTGAT GAAACAGGCA GTGGAGATGT ATGTACATCA TGGGATTTAT 
GATCTAATTT TCAAACAAGT TGGAACAATT GAAGCAAGTG AGGATGCAGC ATTTAACAAG 
ATTACAAGAA GCCTTCAGGA TCTCCAGCAA AAGGATATTG GTGTAAAGAC GGAGTTCAGC 
CTTAACATAC CCAGCGCAAA GCGTGTTCTA TGCCAGTTGA ACAAATGCAC GTCCCCCCAG 
CAAAAACTTT TATGCTTGCG GAAGGTGGTA CAGACTATCA TGCATTCTTC TAGTCAAAGG 
GTGAACATGG AGACTATGTG TGCTGATGAC CTTCTGTCGG TGTTGCTGTA CTTGTTAGTA 
AAAACAGAGA TACCCAACTG GATGGCAAAT TTAAGCTACA TAAAGAATTT TCGTTTTTGC 
AACTCAACAA AAGATGAGTT GGGATACTGC CTAACCTCTG TGGAAGCAGC AATTGAGTTT 
ATCCGTCAAG GAAATTTAAC AGAAACAGCA ACAGCAGAAA CTGAAGGAAA TGATAAGCCA 
TTTTTATGGC AAAGAATGAA CTTAATGTAC AGAGCCTCTT CTACTCCTAT TGATTGCTTA 
TTCAAGCACA TTGCTGCAGG AAATCAAAAA GAAGTAGAGA AGTTACTGAG CCAAGGTGAC 
AGTGATGAAG AAATTGTGCA AAAAATGTGC CACCCACTCT GCTCATGTGA CAACTGTGAA 
AAGCTGGTCT CTGGGAAACT AAATGATACT TCAATTGTTA CCCCATTCTC AAGAGATGAT 
CGAGGTTATA CTCCCCTCCA TATCGCAGCA CTTTATGGTC AGGCTCAGTG TGTTGACTTA 
CTGATATCTA AAGGAGCTGT AGTAAATGCC ACAGACTACC TTGGTTCAAC ACCACTTCAT 
CTTTCGTGCC AGAAAGGCCA CCAGAAGATT GCACTTCTGC TGTTGCATTT TAAGGCATCC 
AGTGACATAC AAGACAATAA CGGCAACACT CCACTACATT TAGCTTGTAC ATATGGACAT 
GAAGATTGTG TGAAAGCTTT AGTCTATTAT GATTTAAACT CCTGTAAAAT TGATACTGTT 
AATGAGAAGG GAGATACCCC TCTCCACATA GCTGCTCGAT GGGGCTACCA AGGAATTATT 
GAGGTCCTGT TAGAGAATGG AGCTAATACT GACATACAGA ACAAGAGAAA GGAAACTCCT 
ATGCAGTGTG CTTTAAATGA AAAGATCCTC ACACTCATGG AAACAGTCTA TGTTACAAAT 
GACCGGAGAC AGAGTATCTC CGAGTCATTG AGCAAATCCC TTCAGTCTGA AGACGCAATC 
AGTGAATCCT CACAATCCAG TTTGTCGTCT GCATCACCTG ACACCAGACC AGAAGATGTG 
AAAAGCAAAT ACAAAGAGGT TGAAAAACTT CTTAGAGCTG TTTCTGATGG TGATCTGGAG 
ATGGTCCGTT ACCTCTTGGA ATGGGTGGAT GATGACTTTG AAGAAGATGA TTTTCCAGTT 
GGGAACAGAG AATTTTGCCA TCCATTATGC CAGTGTACCA AATGTGGTCC TGCTCAGAAG 
AGACTAAGCA AGATTCCACA GAATGGGCTG AATGTCAATG TAACGAGCAG AGATGGCTTC 
ACTCCTCTGC ACATCTCTGC ACTGCAAGGG CATCTTGTTT TAGTGTGTCT CCTTTTAAAG 
CACGGTGCCA GTGTTGATGT AAAGAATGGT AATCGTGCAT TACCTTTGCA CTTGGCCTGC 
CATAAAGGTC ACCTTGAGGT GGTGAAGACT CTAATGGAAT ATAGTACAGG AAAGAATAAA 
AAAGACATGA ATGGAAATAC GCCCTTATTA TACGCATGCA TGGGGGGCCA TCTGGAAATT 
GCATTATTGT TGTTAGAGCA TGGCGCATCT GTTAATATTC GCAATGTAAA AGGGAACACC 
GCACTTCATG AGGCAGTGAG GAGGAATCAC GAGGGTTTAG TGCAGCAGTT GCTCATATAT 
GGTGCACTAG TGGATGCAAG GAACAAACGC CAATATACGC CTTTGGATTA TGCTAAAGAG 
AATTCTAGGA TTAAACAGCT TTTGCTGACA AGCGACAGGA TTGCTGGAGA TGGATTTACT 
ACAGATGAAC ACACCATCCG CATCAGGAAA AAATCAAGCA CCTCCAGTCC TAGAGGAGAA 
GAGGGAGGAC TTACACCAAT GCAACACGAG GTGGCTAAAT CAACAGGTTT GAAAAAAATA 
ATACGGAGAG ACAAAAGTAT GCCTAATATT GATGAGGACC TACTGAATAA GTTATCAGTA 
AAGCAGATAG AGGGCGGTAA CTTAAAACAA ATCAGCAATG ATAAAATGAG CAGAAGCAGC 
GCTGAAGTTC AAGAAGCCCT GGAAGGTGAT GACCTGCCGT CCCCACTTAA AACAAAACTT 
TTTCTGAGAA GACATAGCCT GAATGTTGCT TGTTTAGCAG ATACACAAGA GGACTCTAGT 
GAGGCCACCG ATACCTTAAA TGGGACAGAT GCAAGTTCCC CCTTGTCTGA AACTTGCGAC 
GTGGATTTAG AACATGAAGA TTGCCTAAAC AACCAGGAGT TGTCTGAAAT GAATTGGCAA 
AAGGAGCCCC TTTCTGCATG GACTGCTGTT AAGGAACTAC TAAAAACAGA TGAGTTTGAC 
TGTTCTGGAA AAAAAGACTG TTCTTGTGTG GCTTGTACTT TGGCATTAGA ATCTTCTAGG 
TCTGATCAGT CCTGCTGTGT GAGCTCCGAA CAGGACGGTT TTGATACAGT CCCTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934427.2
CDS	226..3585
Translation	MAMYDEDILNNPFYLAIQKQRPDLIDKVAEAHGIVLVPCKGSVSNILNSTCPFDSYILKPTEGGFETENGKEIFIQGNLVKLGSGFAYHLTIPILFEETFYNEKEESYNILCIARSLEKEENSEEHSTPSKFCTLKNIEDVKEFLGKHAEKFDKSIASFRSTFKVERKSLRHYIDSVNALYTKCLQHLLRDSHLRAIAKQETQMSLMKQAVEMYVHHGIYDLIFKQVGTIEASEDAAFNKITRSLQDLQQKDIGVKTEFSLNIPSAKRVLCQLNKCTSPQQKLLCLRKVVQTIMHSSSQRVNMETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFCNSTKDELGYCLTSVEAAIEFIRQGNLTETATAETEGNDKPFLWQRMNLMYRASSTPIDCLFKHIAAGNQKEVEKLLSQGDSDEEIVQKMCHPLCSCDNCEKLVSGKLNDTSIVTPFSRDDRGYTPLHIAALYGQAQCVDLLISKGAVVNATDYLGSTPLHLSCQKGHQKIALLLLHFKASSDIQDNNGNTPLHLACTYGHEDCVKALVYYDLNSCKIDTVNEKGDTPLHIAARWGYQGIIEVLLENGANTDIQNKRKETPMQCALNEKILTLMETVYVTNDRRQSISESLSKSLQSEDAISESSQSSLSSASPDTRPEDVKSKYKEVEKLLRAVSDGDLEMVRYLLEWVDDDFEEDDFPVGNREFCHPLCQCTKCGPAQKRLSKIPQNGLNVNVTSRDGFTPLHISALQGHLVLVCLLLKHGASVDVKNGNRALPLHLACHKGHLEVVKTLMEYSTGKNKKDMNGNTPLLYACMGGHLEIALLLLEHGASVNIRNVKGNTALHEAVRRNHEGLVQQLLIYGALVDARNKRQYTPLDYAKENSRIKQLLLTSDRIAGDGFTTDEHTIRIRKKSSTSSPRGEEGGLTPMQHEVAKSTGLKKIIRRDKSMPNIDEDLLNKLSVKQIEGGNLKQISNDKMSRSSAEVQEALEGDDLPSPLKTKLFLRRHSLNVACLADTQEDSSEATDTLNGTDASSPLSETCDVDLEHEDCLNNQELSEMNWQKEPLSAWTAVKELLKTDEFDCSGKKDCSCVACTLALESSRSDQSCCVSSEQDGFDTVPL

Target ORF information:

RefSeq Version	XM_002934381.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934381.4

ORF Insert Sequence:

ATGGCTATGT ATGATGAAGA CATTTTGAAC AATCCCTTTT ATTTAGCTAT TCAGAAACAG 
AGACCTGATT TAATTGATAA AGTTGCAGAG GCACATGGAA TTGTCCTGGT TCCATGCAAG 
GGCAGTGTTT CCAACATCTT GAATTCTACA TGTCCTTTTG ATTCGTATAT TTTAAAGCCC 
ACAGAAGGGG GGTTTGAGAC AGAAAATGGA AAGGAGATCT TTATTCAAGG AAACCTGGTT 
AAACTGGGTT CAGGTTTTGC CTACCACCTC ACCATTCCAA TTCTTTTTGA GGAGACTTTC 
TACAATGAAA AAGAGGAAAG CTATAACATT TTGTGTATTG CACGTTCATT AGAAAAAGAG 
GAAAATTCAG AAGAACACTC AACGCCCTCA AAATTTTGCA CTCTGAAAAA TATAGAAGAT 
GTTAAAGAAT TTTTAGGAAA GCACGCTGAG AAGTTTGACA AAAGTATTGC ATCTTTCCGA 
AGCACATTTA AAGTTGAAAG GAAAAGCCTT CGCCATTACA TTGACTCTGT GAATGCCCTG 
TACACAAAAT GTCTTCAGCA TTTACTGCGA GACTCACACT TGAGAGCAAT AGCAAAGCAA 
GAAACGCAAA TGAGCTTGAT GAAACAGGCA GTGGAGATGT ATGTACATCA TGGGATTTAT 
GATCTAATTT TCAAACAAGT TGGAACAATT GAAGCAAGTG AGGATGCAGC ATTTAACAAG 
ATTACAAGAA GCCTTCAGGA TCTCCAGCAA AAGGATATTG GTGTAAAGAC GGAGTTCAGC 
CTTAACATAC CCAGCGCAAA GCGTGTTCTA TGCCAGTTGA ACAAATGCAC GTCCCCCCAG 
CAAAAACTTT TATGCTTGCG GAAGGTGGTA CAGACTATCA TGCATTCTTC TAGTCAAAGG 
GTGAACATGG AGACTATGTG TGCTGATGAC CTTCTGTCGG TGTTGCTGTA CTTGTTAGTA 
AAAACAGAGA TACCCAACTG GATGGCAAAT TTAAGCTACA TAAAGAATTT TCGTTTTTGC 
AACTCAACAA AAGATGAGTT GGGATACTGC CTAACCTCTG TGGAAGCAGC AATTGAGTTT 
ATCCGTCAAG GAAATTTAAC AGAAACAGCA ACAGCAGAAA CTGAAGGAAA TGATAAGCCA 
TTTTTATGGC AAAGAATGAA CTTAATGTAC AGAGCCTCTT CTACTCCTAT TGATTGCTTA 
TTCAAGCACA TTGCTGCAGG AAATCAAAAA GAAGTAGAGA AGTTACTGAG CCAAGGTGAC 
AGTGATGAAG AAATTGTGCA AAAAATGTGC CACCCACTCT GCTCATGTGA CAACTGTGAA 
AAGCTGGTCT CTGGGAAACT AAATGATACT TCAATTGTTA CCCCATTCTC AAGAGATGAT 
CGAGGTTATA CTCCCCTCCA TATCGCAGCA CTTTATGGTC AGGCTCAGTG TGTTGACTTA 
CTGATATCTA AAGGAGCTGT AGTAAATGCC ACAGACTACC TTGGTTCAAC ACCACTTCAT 
CTTTCGTGCC AGAAAGGCCA CCAGAAGATT GCACTTCTGC TGTTGCATTT TAAGGCATCC 
AGTGACATAC AAGACAATAA CGGCAACACT CCACTACATT TAGCTTGTAC ATATGGACAT 
GAAGATTGTG TGAAAGCTTT AGTCTATTAT GATTTAAACT CCTGTAAAAT TGATACTGTT 
AATGAGAAGG GAGATACCCC TCTCCACATA GCTGCTCGAT GGGGCTACCA AGGAATTATT 
GAGGTCCTGT TAGAGAATGG AGCTAATACT GACATACAGA ACAAGAGAAA GGAAACTCCT 
ATGCAGTGTG CTTTAAATGA AAAGATCCTC ACACTCATGG AAACAGTCTA TGTTACAAAT 
GACCGGAGAC AGAGTATCTC CGAGTCATTG AGCAAATCCC TTCAGTCTGA AGACGCAATC 
AGTGAATCCT CACAATCCAG TTTGTCGTCT GCATCACCTG ACACCAGACC AGAAGATGTG 
AAAAGCAAAT ACAAAGAGGT TGAAAAACTT CTTAGAGCTG TTTCTGATGG TGATCTGGAG 
ATGGTCCGTT ACCTCTTGGA ATGGGTGGAT GATGACTTTG AAGAAGATGA TTTTCCAGTT 
GGGAACAGAG AATTTTGCCA TCCATTATGC CAGTGTACCA AATGTGGTCC TGCTCAGAAG 
AGACTAAGCA AGATTCCACA GAATGGGCTG AATGTCAATG TAACGAGCAG AGATGGCTTC 
ACTCCTCTGC ACATCTCTGC ACTGCAAGGG CATCTTGTTT TAGTGTGTCT CCTTTTAAAG 
CACGGTGCCA GTGTTGATGT AAAGAATGGT AATCGTGCAT TACCTTTGCA CTTGGCCTGC 
CATAAAGGTC ACCTTGAGGT GGTGAAGACT CTAATGGAAT ATAGTACAGG AAAGAATAAA 
AAAGACATGA ATGGAAATAC GCCCTTATTA TACGCATGCA TGGGGGGCCA TCTGGAAATT 
GCATTATTGT TGTTAGAGCA TGGCGCATCT GTTAATATTC GCAATGTAAA AGGGAACACC 
GCACTTCATG AGGCAGTGAG GAGGAATCAC GAGGGTTTAG TGCAGCAGTT GCTCATATAT 
GGTGCACTAG TGGATGCAAG GAACAAACGC CAATATACGC CTTTGGATTA TGCTAAAGAG 
AATTCTAGGA TTAAACAGCT TTTGCTGACA AGCGACAGGA TTGCTGGAGA TGGATTTACT 
ACAGATGAAC ACACCATCCG CATCAGGAAA AAATCAAGCA CCTCCAGTCC TAGAGGAGAA 
GAGGGAGGAC TTACACCAAT GCAACACGAG GTGGCTAAAT CAACAGGTTT GAAAAAAATA 
ATACGGAGAG ACAAAAGTAT GCCTAATATT GATGAGGACC TACTGAATAA GTTATCAGTA 
AAGCAGATAG AGGGCGGTAA CTTAAAACAA ATCAGCAATG ATAAAATGAG CAGAAGCAGC 
GCTGAAGTTC AAGAAGCCCT GGAAGGTGAT GACCTGCCGT CCCCACTTAA AACAAAACTT 
TTTCTGAGAA GACATAGCCT GAATGTTGCT TGTTTAGCAG ATACACAAGA GGACTCTAGT 
GAGGCCACCG ATACCTTAAA TGGGACAGAT GCAAGTTCCC CCTTGTCTGA AACTTGCGAC 
GTGGATTTAG AACATGAAGA TTGCCTAAAC AACCAGGAGT TGTCTGAAAT GAATTGGCAA 
AAGGAGCCCC TTTCTGCATG GACTGCTGTT AAGGAACTAC TAAAAACAGA TGAGTTTGAC 
TGTTCTGGAA AAAAAGACTG TTCTTGTGTG GCTTGTACTT TGGCATTAGA ATCTTCTAGG 
TCTGATCAGT CCTGCTGTGT GAGCTCCGAA CAGGACGGTT TTGATACAGT CCCTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankrd27 ( XM_004913553.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913610.1 Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa08794
Clone ID Related Accession (Same CDS sequence)	XM_002934381.4 , XM_004913553.3
Accession Version	XM_004913553.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat domain-containing protein 27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913553.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATGT ATGATGAAGA CATTTTGAAC AATCCCTTTT ATTTAGCTAT TCAGAAACAG 
AGACCTGATT TAATTGATAA AGTTGCAGAG GCACATGGAA TTGTCCTGGT TCCATGCAAG 
GGCAGTGTTT CCAACATCTT GAATTCTACA TGTCCTTTTG ATTCGTATAT TTTAAAGCCC 
ACAGAAGGGG GGTTTGAGAC AGAAAATGGA AAGGAGATCT TTATTCAAGG AAACCTGGTT 
AAACTGGGTT CAGGTTTTGC CTACCACCTC ACCATTCCAA TTCTTTTTGA GGAGACTTTC 
TACAATGAAA AAGAGGAAAG CTATAACATT TTGTGTATTG CACGTTCATT AGAAAAAGAG 
GAAAATTCAG AAGAACACTC AACGCCCTCA AAATTTTGCA CTCTGAAAAA TATAGAAGAT 
GTTAAAGAAT TTTTAGGAAA GCACGCTGAG AAGTTTGACA AAAGTATTGC ATCTTTCCGA 
AGCACATTTA AAGTTGAAAG GAAAAGCCTT CGCCATTACA TTGACTCTGT GAATGCCCTG 
TACACAAAAT GTCTTCAGCA TTTACTGCGA GACTCACACT TGAGAGCAAT AGCAAAGCAA 
GAAACGCAAA TGAGCTTGAT GAAACAGGCA GTGGAGATGT ATGTACATCA TGGGATTTAT 
GATCTAATTT TCAAACAAGT TGGAACAATT GAAGCAAGTG AGGATGCAGC ATTTAACAAG 
ATTACAAGAA GCCTTCAGGA TCTCCAGCAA AAGGATATTG GTGTAAAGAC GGAGTTCAGC 
CTTAACATAC CCAGCGCAAA GCGTGTTCTA TGCCAGTTGA ACAAATGCAC GTCCCCCCAG 
CAAAAACTTT TATGCTTGCG GAAGGTGGTA CAGACTATCA TGCATTCTTC TAGTCAAAGG 
GTGAACATGG AGACTATGTG TGCTGATGAC CTTCTGTCGG TGTTGCTGTA CTTGTTAGTA 
AAAACAGAGA TACCCAACTG GATGGCAAAT TTAAGCTACA TAAAGAATTT TCGTTTTTGC 
AACTCAACAA AAGATGAGTT GGGATACTGC CTAACCTCTG TGGAAGCAGC AATTGAGTTT 
ATCCGTCAAG GAAATTTAAC AGAAACAGCA ACAGCAGAAA CTGAAGGAAA TGATAAGCCA 
TTTTTATGGC AAAGAATGAA CTTAATGTAC AGAGCCTCTT CTACTCCTAT TGATTGCTTA 
TTCAAGCACA TTGCTGCAGG AAATCAAAAA GAAGTAGAGA AGTTACTGAG CCAAGGTGAC 
AGTGATGAAG AAATTGTGCA AAAAATGTGC CACCCACTCT GCTCATGTGA CAACTGTGAA 
AAGCTGGTCT CTGGGAAACT AAATGATACT TCAATTGTTA CCCCATTCTC AAGAGATGAT 
CGAGGTTATA CTCCCCTCCA TATCGCAGCA CTTTATGGTC AGGCTCAGTG TGTTGACTTA 
CTGATATCTA AAGGAGCTGT AGTAAATGCC ACAGACTACC TTGGTTCAAC ACCACTTCAT 
CTTTCGTGCC AGAAAGGCCA CCAGAAGATT GCACTTCTGC TGTTGCATTT TAAGGCATCC 
AGTGACATAC AAGACAATAA CGGCAACACT CCACTACATT TAGCTTGTAC ATATGGACAT 
GAAGATTGTG TGAAAGCTTT AGTCTATTAT GATTTAAACT CCTGTAAAAT TGATACTGTT 
AATGAGAAGG GAGATACCCC TCTCCACATA GCTGCTCGAT GGGGCTACCA AGGAATTATT 
GAGGTCCTGT TAGAGAATGG AGCTAATACT GACATACAGA ACAAGAGAAA GGAAACTCCT 
ATGCAGTGTG CTTTAAATGA AAAGATCCTC ACACTCATGG AAACAGTCTA TGTTACAAAT 
GACCGGAGAC AGAGTATCTC CGAGTCATTG AGCAAATCCC TTCAGTCTGA AGACGCAATC 
AGTGAATCCT CACAATCCAG TTTGTCGTCT GCATCACCTG ACACCAGACC AGAAGATGTG 
AAAAGCAAAT ACAAAGAGGT TGAAAAACTT CTTAGAGCTG TTTCTGATGG TGATCTGGAG 
ATGGTCCGTT ACCTCTTGGA ATGGGTGGAT GATGACTTTG AAGAAGATGA TTTTCCAGTT 
GGGAACAGAG AATTTTGCCA TCCATTATGC CAGTGTACCA AATGTGGTCC TGCTCAGAAG 
AGACTAAGCA AGATTCCACA GAATGGGCTG AATGTCAATG TAACGAGCAG AGATGGCTTC 
ACTCCTCTGC ACATCTCTGC ACTGCAAGGG CATCTTGTTT TAGTGTGTCT CCTTTTAAAG 
CACGGTGCCA GTGTTGATGT AAAGAATGGT AATCGTGCAT TACCTTTGCA CTTGGCCTGC 
CATAAAGGTC ACCTTGAGGT GGTGAAGACT CTAATGGAAT ATAGTACAGG AAAGAATAAA 
AAAGACATGA ATGGAAATAC GCCCTTATTA TACGCATGCA TGGGGGGCCA TCTGGAAATT 
GCATTATTGT TGTTAGAGCA TGGCGCATCT GTTAATATTC GCAATGTAAA AGGGAACACC 
GCACTTCATG AGGCAGTGAG GAGGAATCAC GAGGGTTTAG TGCAGCAGTT GCTCATATAT 
GGTGCACTAG TGGATGCAAG GAACAAACGC CAATATACGC CTTTGGATTA TGCTAAAGAG 
AATTCTAGGA TTAAACAGCT TTTGCTGACA AGCGACAGGA TTGCTGGAGA TGGATTTACT 
ACAGATGAAC ACACCATCCG CATCAGGAAA AAATCAAGCA CCTCCAGTCC TAGAGGAGAA 
GAGGGAGGAC TTACACCAAT GCAACACGAG GTGGCTAAAT CAACAGGTTT GAAAAAAATA 
ATACGGAGAG ACAAAAGTAT GCCTAATATT GATGAGGACC TACTGAATAA GTTATCAGTA 
AAGCAGATAG AGGGCGGTAA CTTAAAACAA ATCAGCAATG ATAAAATGAG CAGAAGCAGC 
GCTGAAGTTC AAGAAGCCCT GGAAGGTGAT GACCTGCCGT CCCCACTTAA AACAAAACTT 
TTTCTGAGAA GACATAGCCT GAATGTTGCT TGTTTAGCAG ATACACAAGA GGACTCTAGT 
GAGGCCACCG ATACCTTAAA TGGGACAGAT GCAAGTTCCC CCTTGTCTGA AACTTGCGAC 
GTGGATTTAG AACATGAAGA TTGCCTAAAC AACCAGGAGT TGTCTGAAAT GAATTGGCAA 
AAGGAGCCCC TTTCTGCATG GACTGCTGTT AAGGAACTAC TAAAAACAGA TGAGTTTGAC 
TGTTCTGGAA AAAAAGACTG TTCTTGTGTG GCTTGTACTT TGGCATTAGA ATCTTCTAGG 
TCTGATCAGT CCTGCTGTGT GAGCTCCGAA CAGGACGGTT TTGATACAGT CCCTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913610.1
CDS	412..3771
Translation	MAMYDEDILNNPFYLAIQKQRPDLIDKVAEAHGIVLVPCKGSVSNILNSTCPFDSYILKPTEGGFETENGKEIFIQGNLVKLGSGFAYHLTIPILFEETFYNEKEESYNILCIARSLEKEENSEEHSTPSKFCTLKNIEDVKEFLGKHAEKFDKSIASFRSTFKVERKSLRHYIDSVNALYTKCLQHLLRDSHLRAIAKQETQMSLMKQAVEMYVHHGIYDLIFKQVGTIEASEDAAFNKITRSLQDLQQKDIGVKTEFSLNIPSAKRVLCQLNKCTSPQQKLLCLRKVVQTIMHSSSQRVNMETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFCNSTKDELGYCLTSVEAAIEFIRQGNLTETATAETEGNDKPFLWQRMNLMYRASSTPIDCLFKHIAAGNQKEVEKLLSQGDSDEEIVQKMCHPLCSCDNCEKLVSGKLNDTSIVTPFSRDDRGYTPLHIAALYGQAQCVDLLISKGAVVNATDYLGSTPLHLSCQKGHQKIALLLLHFKASSDIQDNNGNTPLHLACTYGHEDCVKALVYYDLNSCKIDTVNEKGDTPLHIAARWGYQGIIEVLLENGANTDIQNKRKETPMQCALNEKILTLMETVYVTNDRRQSISESLSKSLQSEDAISESSQSSLSSASPDTRPEDVKSKYKEVEKLLRAVSDGDLEMVRYLLEWVDDDFEEDDFPVGNREFCHPLCQCTKCGPAQKRLSKIPQNGLNVNVTSRDGFTPLHISALQGHLVLVCLLLKHGASVDVKNGNRALPLHLACHKGHLEVVKTLMEYSTGKNKKDMNGNTPLLYACMGGHLEIALLLLEHGASVNIRNVKGNTALHEAVRRNHEGLVQQLLIYGALVDARNKRQYTPLDYAKENSRIKQLLLTSDRIAGDGFTTDEHTIRIRKKSSTSSPRGEEGGLTPMQHEVAKSTGLKKIIRRDKSMPNIDEDLLNKLSVKQIEGGNLKQISNDKMSRSSAEVQEALEGDDLPSPLKTKLFLRRHSLNVACLADTQEDSSEATDTLNGTDASSPLSETCDVDLEHEDCLNNQELSEMNWQKEPLSAWTAVKELLKTDEFDCSGKKDCSCVACTLALESSRSDQSCCVSSEQDGFDTVPL

Target ORF information:

RefSeq Version	XM_004913553.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat domain 27 (ankrd27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913553.3

ORF Insert Sequence:

ATGGCTATGT ATGATGAAGA CATTTTGAAC AATCCCTTTT ATTTAGCTAT TCAGAAACAG 
AGACCTGATT TAATTGATAA AGTTGCAGAG GCACATGGAA TTGTCCTGGT TCCATGCAAG 
GGCAGTGTTT CCAACATCTT GAATTCTACA TGTCCTTTTG ATTCGTATAT TTTAAAGCCC 
ACAGAAGGGG GGTTTGAGAC AGAAAATGGA AAGGAGATCT TTATTCAAGG AAACCTGGTT 
AAACTGGGTT CAGGTTTTGC CTACCACCTC ACCATTCCAA TTCTTTTTGA GGAGACTTTC 
TACAATGAAA AAGAGGAAAG CTATAACATT TTGTGTATTG CACGTTCATT AGAAAAAGAG 
GAAAATTCAG AAGAACACTC AACGCCCTCA AAATTTTGCA CTCTGAAAAA TATAGAAGAT 
GTTAAAGAAT TTTTAGGAAA GCACGCTGAG AAGTTTGACA AAAGTATTGC ATCTTTCCGA 
AGCACATTTA AAGTTGAAAG GAAAAGCCTT CGCCATTACA TTGACTCTGT GAATGCCCTG 
TACACAAAAT GTCTTCAGCA TTTACTGCGA GACTCACACT TGAGAGCAAT AGCAAAGCAA 
GAAACGCAAA TGAGCTTGAT GAAACAGGCA GTGGAGATGT ATGTACATCA TGGGATTTAT 
GATCTAATTT TCAAACAAGT TGGAACAATT GAAGCAAGTG AGGATGCAGC ATTTAACAAG 
ATTACAAGAA GCCTTCAGGA TCTCCAGCAA AAGGATATTG GTGTAAAGAC GGAGTTCAGC 
CTTAACATAC CCAGCGCAAA GCGTGTTCTA TGCCAGTTGA ACAAATGCAC GTCCCCCCAG 
CAAAAACTTT TATGCTTGCG GAAGGTGGTA CAGACTATCA TGCATTCTTC TAGTCAAAGG 
GTGAACATGG AGACTATGTG TGCTGATGAC CTTCTGTCGG TGTTGCTGTA CTTGTTAGTA 
AAAACAGAGA TACCCAACTG GATGGCAAAT TTAAGCTACA TAAAGAATTT TCGTTTTTGC 
AACTCAACAA AAGATGAGTT GGGATACTGC CTAACCTCTG TGGAAGCAGC AATTGAGTTT 
ATCCGTCAAG GAAATTTAAC AGAAACAGCA ACAGCAGAAA CTGAAGGAAA TGATAAGCCA 
TTTTTATGGC AAAGAATGAA CTTAATGTAC AGAGCCTCTT CTACTCCTAT TGATTGCTTA 
TTCAAGCACA TTGCTGCAGG AAATCAAAAA GAAGTAGAGA AGTTACTGAG CCAAGGTGAC 
AGTGATGAAG AAATTGTGCA AAAAATGTGC CACCCACTCT GCTCATGTGA CAACTGTGAA 
AAGCTGGTCT CTGGGAAACT AAATGATACT TCAATTGTTA CCCCATTCTC AAGAGATGAT 
CGAGGTTATA CTCCCCTCCA TATCGCAGCA CTTTATGGTC AGGCTCAGTG TGTTGACTTA 
CTGATATCTA AAGGAGCTGT AGTAAATGCC ACAGACTACC TTGGTTCAAC ACCACTTCAT 
CTTTCGTGCC AGAAAGGCCA CCAGAAGATT GCACTTCTGC TGTTGCATTT TAAGGCATCC 
AGTGACATAC AAGACAATAA CGGCAACACT CCACTACATT TAGCTTGTAC ATATGGACAT 
GAAGATTGTG TGAAAGCTTT AGTCTATTAT GATTTAAACT CCTGTAAAAT TGATACTGTT 
AATGAGAAGG GAGATACCCC TCTCCACATA GCTGCTCGAT GGGGCTACCA AGGAATTATT 
GAGGTCCTGT TAGAGAATGG AGCTAATACT GACATACAGA ACAAGAGAAA GGAAACTCCT 
ATGCAGTGTG CTTTAAATGA AAAGATCCTC ACACTCATGG AAACAGTCTA TGTTACAAAT 
GACCGGAGAC AGAGTATCTC CGAGTCATTG AGCAAATCCC TTCAGTCTGA AGACGCAATC 
AGTGAATCCT CACAATCCAG TTTGTCGTCT GCATCACCTG ACACCAGACC AGAAGATGTG 
AAAAGCAAAT ACAAAGAGGT TGAAAAACTT CTTAGAGCTG TTTCTGATGG TGATCTGGAG 
ATGGTCCGTT ACCTCTTGGA ATGGGTGGAT GATGACTTTG AAGAAGATGA TTTTCCAGTT 
GGGAACAGAG AATTTTGCCA TCCATTATGC CAGTGTACCA AATGTGGTCC TGCTCAGAAG 
AGACTAAGCA AGATTCCACA GAATGGGCTG AATGTCAATG TAACGAGCAG AGATGGCTTC 
ACTCCTCTGC ACATCTCTGC ACTGCAAGGG CATCTTGTTT TAGTGTGTCT CCTTTTAAAG 
CACGGTGCCA GTGTTGATGT AAAGAATGGT AATCGTGCAT TACCTTTGCA CTTGGCCTGC 
CATAAAGGTC ACCTTGAGGT GGTGAAGACT CTAATGGAAT ATAGTACAGG AAAGAATAAA 
AAAGACATGA ATGGAAATAC GCCCTTATTA TACGCATGCA TGGGGGGCCA TCTGGAAATT 
GCATTATTGT TGTTAGAGCA TGGCGCATCT GTTAATATTC GCAATGTAAA AGGGAACACC 
GCACTTCATG AGGCAGTGAG GAGGAATCAC GAGGGTTTAG TGCAGCAGTT GCTCATATAT 
GGTGCACTAG TGGATGCAAG GAACAAACGC CAATATACGC CTTTGGATTA TGCTAAAGAG 
AATTCTAGGA TTAAACAGCT TTTGCTGACA AGCGACAGGA TTGCTGGAGA TGGATTTACT 
ACAGATGAAC ACACCATCCG CATCAGGAAA AAATCAAGCA CCTCCAGTCC TAGAGGAGAA 
GAGGGAGGAC TTACACCAAT GCAACACGAG GTGGCTAAAT CAACAGGTTT GAAAAAAATA 
ATACGGAGAG ACAAAAGTAT GCCTAATATT GATGAGGACC TACTGAATAA GTTATCAGTA 
AAGCAGATAG AGGGCGGTAA CTTAAAACAA ATCAGCAATG ATAAAATGAG CAGAAGCAGC 
GCTGAAGTTC AAGAAGCCCT GGAAGGTGAT GACCTGCCGT CCCCACTTAA AACAAAACTT 
TTTCTGAGAA GACATAGCCT GAATGTTGCT TGTTTAGCAG ATACACAAGA GGACTCTAGT 
GAGGCCACCG ATACCTTAAA TGGGACAGAT GCAAGTTCCC CCTTGTCTGA AACTTGCGAC 
GTGGATTTAG AACATGAAGA TTGCCTAAAC AACCAGGAGT TGTCTGAAAT GAATTGGCAA 
AAGGAGCCCC TTTCTGCATG GACTGCTGTT AAGGAACTAC TAAAAACAGA TGAGTTTGAC 
TGTTCTGGAA AAAAAGACTG TTCTTGTGTG GCTTGTACTT TGGCATTAGA ATCTTCTAGG 
TCTGATCAGT CCTGCTGTGT GAGCTCCGAA CAGGACGGTT TTGATACAGT CCCTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ankrd27 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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