ccdc180 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ccdc180
Entrez Gene ID	100492567
Full Name	coiled-coil domain containing 180
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012968714.2	XP_012824168.1	coiled-coil domain-containing protein 180
XM_031891402.1	XP_031747262.1	coiled-coil domain-containing protein 180 isoform X1
XM_031891403.1	XP_031747263.1	coiled-coil domain-containing protein 180 isoform X2
XM_012968714.3	XP_012824168.2	coiled-coil domain-containing protein 180 isoform X1
XM_031891404.1	XP_031747264.1	coiled-coil domain-containing protein 180 isoform X3
XM_031891405.1	XP_031747265.1	coiled-coil domain-containing protein 180 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	CCDC180	NP_065944.2
Rattus norvegicus (Norway rat)	Ccdc180	XP_006238214.1
Danio rerio (zebrafish)	LOC100538012	XP_003198708.2
Pan troglodytes (chimpanzee)	LOC464596	XP_520133.2
Canis lupus familiaris (dog)	C11H9orf174	XP_005627048.1
Bos taurus (cattle)	CCDC180	XP_005210245.1
Mus musculus (house mouse)	E230008N13Rik	NP_941062.2
Xenopus tropicalis (tropical clawed frog)	LOC100492567	XP_002941145.2
Gallus gallus (chicken)	C17H9ORF174	XP_004945990.1

Gene Ontology

The following ccdc180 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ccdc180 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa64701	XM_031891402.1 Latest version!	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa64701	XM_012968714.3 Latest version!	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa64702	XM_031891403.1 Latest version!	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa64703	XM_031891404.1 Latest version!	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa64704	XM_031891405.1 Latest version!	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa35406	XM_012968714.2 Latest version!	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ccdc180 ( XM_031891402.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747262.1 Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa64701
Clone ID Related Accession (Same CDS sequence)	XM_031891402.1 , XM_012968714.3
Accession Version	XM_031891402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	coiled-coil domain-containing protein 180 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG TGGGACAGAT CCAGGCAGTT CCTAGTGGAA GAGTCTACAG GCAACTTTTT 
GATGCTGAGG TTCAGCTTGT GCGGTCCCTC GGAGAGGCTC GTGCTAGAGC GGTGCAGCAT 
GGAGGTGCAG TGGACACTGG GAACATTCCT CCAATCAGAA GCCGGGATAA CAACACAGCA 
TTGCCTGAGT CTCACCACCA GAGCACGGAT AAAATGACTC CACCTGCTAG GAAAAAGCAG 
GAAAGTGAGG ATGATATTGC AGCAAGAGAA GTGAGAGGTC TTTCTGATGT CATCGTTCCA 
GAAAAGAAAG ATACCGGCAT TCTTGAGCGG ATCGCTGCAA ACAGACTGGA ACGCCATGAA 
GTGGCACTGT CCAACCTGCA CAGGGAACTG GCTGAGATCA GCAAAGAGAT GGAGCTATCA 
GTATTGGAAG TGGGGAAGCA CCTGCAGCAA AAGATCTCGG AATCAGATGC AAATATTGAA 
CTTTTGTTTC AGAAACTGGA AATCGGTAAC GACAGCACCA CCACAAAGGA AATAAATGAG 
ACTTGGGATG CAGTGACCCA GGAATCTGCC CGTACAAGGC AGTGCATCAA AAGAACCGAA 
AGCACTTTAC TGGCGCTAGA AGAAAACAGA GCGAAACGAA TTTCTGAAGT CTTAAAGAAA 
TACACGTCCC TCTTAAAGGA CATCTGCCAT CTCATGCCCT CCGACGTGGA CAGATTTATT 
CATAATGAAG CCATGATGAT TAACCAGGCA ATTTTAGCTA ACCACCGAGC TCTGGCTAGA 
TTATCTGTAA ACCTTCTGGA AGCCAATCTG AAGAGGGAGA ATACCCAGCG CTTCCAATGG 
CAGGACCTGA AGAAGGCCTG GGAGTGTTAC CAGAGGGAGA TCATAATGAA GGAGTTCAGG 
GTGTTTGCTG AGAAAGAAAT GGAACATATA CCCACAAGTG TGAAAGTATT AGTGGATCAG 
CTGACCACCC AACTCAGCTC TCTCAAAGAA AGGAGACTTC AGATTCTGGG TTTAGCCAGA 
AGTTTTGTAC CTCCTACTTG CACGAGAACA GGTGTCATGG AATGGTATGA ATCGTTGGCA 
GCCCTGAACA GTCAAGCTGA GTCCATCAAC AAACAATTCT TAGAGAAGCT TCAGGAGCTG 
CGGGAGGAAT TCTGCCAAAA ATTTGAGGTG AAAGCAGAAA GCTGTAAAGA GCAGTTGGTC 
AGTGTGGATA TTTGTACCCC AGAGGAAGCT GAGGATATAA TAACCAAAGA ACTCAGCCCA 
GTGACGGCAC AGATCCACAT GAAATTTAAG GAAGAGCAAG AGAAGTGGGA AAAACTGCTG 
GTACATAGCT CTAAGTTAAC GATCCTTCAG ACCCAGAAGT TGCTGAAATT CTTGGAAGAT 
GTTGTTGATA TTTGGCAAAC GCTGGAACGT GGCCTCTCCA AACAGGAAAA TGCTGTGCAG 
CTAAGGATGG AGCAGTGTCG CTATAAATAT GATAGTGACA GCAAGGCCAA GGAGGCAAAT 
TTGGACAAGA TACTGGACAG ACTGAGACAA GACAGCACTA AGGAAACATT ACGTAGCACA 
TTGGGGAAAG CACTGGCGTC CTTGGAAACC ATAAAAGCAG GATACAAGAC TTTTCATTTG 
GACCAAGTCG CTGTAATTGA TTCCTACCCT GCCCTGGTGC TTGCAGAGTT GGCCTCCTAC 
AGCTGCGCTC TTAGCAATTA TTTTGGGGTC AAAGAGGTGT ATGGGCAGGT GCAGAAGAAG 
GATGGGGAGA ACCCCAGCAC CAGTATAACA ACGATTCCGG CAGATGGAGT GAGGTCTCAT 
TCTAGAGAGG GGATATCAGT AAACTCCGAA TTTAATTCCT ACAACTATTC TGCAGTAGAA 
AGTGTTTTGC TGGAAGGTGA ATGGCTGGAA GGGCCAGGAG ATGGAACCCA CAACACAAGA 
GAAGACACGG AAGTGCCTGG AATAGTACAG TGTGATTCTA CTATGGCACC AGTGCAAACC 
CCTTATCGGG AGAGCACCCA AGCAGCAGAG GGAGCTGGTG AGCAGACATC ACAGGGAGCC 
CAGATTCCAT TTGAGCTCTT CACAACATCT AAAGGGAACA CCTATACTGC TTTGCGTTTG 
GCATCAGAAG ATGACACGGC GGTGCCTGAG TTTCCAGGAG AACATTCCGA CACAAATATA 
TTCATGACGG AGGCGGTGCT GGACGATTCC GTTCCCTTAA ATCATGACAT AATGGTCATA 
CCCGAGCAAC AGTTGATTGT TATTAGGAAA AGCGTCCGGC TTGGGTTCTT TGAGCACCTG 
GAGACCTGGT TCGGTGAGAC CCTTTCCAGT GCCCAGGGTA TCGTCAGTGC CAAGAAGGAA 
GAGTTCAATT CTGAGTTAGC GCTTCGCTAT CACCTGCACG AGCCAAGGAG CCAACGGATC 
GAGATGGATA TCCATAACGT CAGAGCTGCC GAGCTGATTC TGCACTCCGA GAGAGTGGAT 
CGTCACTGTG ATGGGGTAAG CCATTCTCTG GAGCAGCTGA AGCGGGAGAG TTCCGTGCTG 
ATTGAGAACA TGAAGCAACA GACTCTCGGA TTCCGCAGCA AGATCCGGGC CATGGAGAGC 
ATGTTCCTCG GTGCCAACAA GTCTGATAAG CTTGTGGCCC TGACACAGTC TCTGCCGTCC 
ATTTTGGACA GCCACGTAAG CGGGGTTCAG ACTTCCATGC GCAACTACCG GCAGCATGTA 
GAGGAAATGC TCGGAACCCT GCGCGACACA AACTCGGACT TCATCAAATC ATTCAGATTG 
TTCTCAGAGG GCGGAAACTT TTCTCCCGAT GAAGTGGAGG TGTTTCGCAA GAAGCTGCAT 
AAAGCGTCAG ACACCATCGC CTCTTTCGAG GGCTCCATCA TGGTGGATTT GGAGGGCTTG 
GAGTCTGTGT GTTTAGAACA GGCCACTGAG ATTGTAAAGA AATTTGAGGA CAAATTCAAC 
ATGTTAACAA CGGATGCAAT TTTCCTAGAA AACATTCAGA AGCTGCTGAC GAACCTGCAA 
GTGAAAATCA AAGCCTTGGT TGTGAGCAGC AATTTTCAGA CTCAGCAGAT CAACTCCTCT 
CTGGAGCAGC TCCAACAGAA GACAGATGCC TGCGCTCATC CCAATATGGA TAAGGAAACA 
GTCACAGCTG AAGAACTGTA CTCCTTTGTG CAAATCCCCA TGGGTCAGCT GGCAAAGAGA 
AGTCTCTATC TGTCCTGCCT TCTAGAGCCC AGCCAAATAC TCTCTGAGAC CCCACTGCAA 
GGCCCTATTG CAACGGCATC ACGTTCGGAC ACCCCACTCC GTCAAGACGC GAAAGGAACC 
TTTACGACCC CCGACAACCT TCTTACTCCG AGCAGAGTGG GGAAGTTGGC TCTCGATGAT 
CCCGCTGTGA GTGTCATCAA GAACATTACC AGAACAAAGC GGGTTATGGA TTCACAACAG 
GACTCTGACC GTGCAAGTAG CTATCAGCGG CCGGGCACAG TCTCACATCA GGCTGCTGCC 
ATAGCGCCAC ATCCACCATC GCAGTCGGGT AAACCTCATC TGAAGAAAAA GAAACCCCCA 
TCAAGCAATG GTGAAAATCC TGGCCAGTCT GTTCAGGCTC CAAGTGTCCG GAAGCTTACA 
AAGCCCAGCA GGTTTGACAA GAAGTACCAA TTGTTTGGGG AGAACAGAGT CGAATCAGAC 
AACTTCAAGG GAATTCTCAC CTGTATACTG TGGGAGAGCA ATGACACCTT ACTGTACCTG 
GCTGAGGAGT TCTATAAGAA GAAGGACAGG CGGAACATTG GCCGGACAGA TCTTCTTCAA 
GAGACGTTTG AGGAGTGTGC AGATACGCTG GTGTTTAAAC TGCAGTCCTA CGAGAAGCAG 
GCCCTGGAGT ATCAGAACAA CTGCCTGCTG GAGTTCCGGG AGCAGCTACA AACTTTCGAG 
AGGCTGGTAA ATGAGGTGCC GCCGCTGGTC ATAGAGAGTC ACCGGAGGCA ACACCTGGAC 
AAACTGCTGG CGGCCACTGA CCAGATACGG AAGCAGTTTG CTGAGGACCT GCAGCGCTGG 
GACCAAGCCA AGGAGACACT GAAGAGTGTG CTGAGGCCAA ATTTAGGCCA CCCAGACAGC 
GAGCAGGCCC TGGGGGAGCT GTGCCAGCAG GAGGAACTAA GGCAGAGAGA GGAGGCAGAA 
GGGATTGACT GCAACACTAA ACAATTACAA GCGTGCGTCT CCGAGTGCAC CCAGCACTTC 
CTGATGTCTC TGGCCTCCCT CACCGAGAGA CTGCTGCTGG AACTCGATGA GACACTTACA 
GTCGATGATG TTTTAGCAGC TGATGTTGAG ATTCCCAAAG AAAAACTGTC AACGCTGATC 
AGACGCAAGC AGGCGGGGCT GCCGTTGGAA AGCACAGAGC ACAAGCCACT GGTGGAGAGA 
GGCAGCAGGG TCTGGCCAGG AATTGGAATT GTGGATCCTA CAGACCCTGA AGGGGACAGT 
GCGCGCCACA TCAGCGCATC GGTCACCACG GCCAAGACCA CAGTCGGCCA CATCTCAGCG 
GTGCAGGCTC GCGACTCCAC TCGCTCGCGG TTCCTGCAGG ACACAGAGGC GGTACTGACC 
CGTATCCGGG AGGAGAGCCG GCAGCAGCAC TTTGATGCCC AGCGCTGGAG GGATTGGTGG 
TGCCAGTCTG TACGCAATAT AAAAGCTCTG TATATGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747262.1
CDS	206..4867
Translation	MAAVGQIQAVPSGRVYRQLFDAEVQLVRSLGEARARAVQHGGAVDTGNIPPIRSRDNNTALPESHHQSTDKMTPPARKKQESEDDIAAREVRGLSDVIVPEKKDTGILERIAANRLERHEVALSNLHRELAEISKEMELSVLEVGKHLQQKISESDANIELLFQKLEIGNDSTTTKEINETWDAVTQESARTRQCIKRTESTLLALEENRAKRISEVLKKYTSLLKDICHLMPSDVDRFIHNEAMMINQAILANHRALARLSVNLLEANLKRENTQRFQWQDLKKAWECYQREIIMKEFRVFAEKEMEHIPTSVKVLVDQLTTQLSSLKERRLQILGLARSFVPPTCTRTGVMEWYESLAALNSQAESINKQFLEKLQELREEFCQKFEVKAESCKEQLVSVDICTPEEAEDIITKELSPVTAQIHMKFKEEQEKWEKLLVHSSKLTILQTQKLLKFLEDVVDIWQTLERGLSKQENAVQLRMEQCRYKYDSDSKAKEANLDKILDRLRQDSTKETLRSTLGKALASLETIKAGYKTFHLDQVAVIDSYPALVLAELASYSCALSNYFGVKEVYGQVQKKDGENPSTSITTIPADGVRSHSREGISVNSEFNSYNYSAVESVLLEGEWLEGPGDGTHNTREDTEVPGIVQCDSTMAPVQTPYRESTQAAEGAGEQTSQGAQIPFELFTTSKGNTYTALRLASEDDTAVPEFPGEHSDTNIFMTEAVLDDSVPLNHDIMVIPEQQLIVIRKSVRLGFFEHLETWFGETLSSAQGIVSAKKEEFNSELALRYHLHEPRSQRIEMDIHNVRAAELILHSERVDRHCDGVSHSLEQLKRESSVLIENMKQQTLGFRSKIRAMESMFLGANKSDKLVALTQSLPSILDSHVSGVQTSMRNYRQHVEEMLGTLRDTNSDFIKSFRLFSEGGNFSPDEVEVFRKKLHKASDTIASFEGSIMVDLEGLESVCLEQATEIVKKFEDKFNMLTTDAIFLENIQKLLTNLQVKIKALVVSSNFQTQQINSSLEQLQQKTDACAHPNMDKETVTAEELYSFVQIPMGQLAKRSLYLSCLLEPSQILSETPLQGPIATASRSDTPLRQDAKGTFTTPDNLLTPSRVGKLALDDPAVSVIKNITRTKRVMDSQQDSDRASSYQRPGTVSHQAAAIAPHPPSQSGKPHLKKKKPPSSNGENPGQSVQAPSVRKLTKPSRFDKKYQLFGENRVESDNFKGILTCILWESNDTLLYLAEEFYKKKDRRNIGRTDLLQETFEECADTLVFKLQSYEKQALEYQNNCLLEFREQLQTFERLVNEVPPLVIESHRRQHLDKLLAATDQIRKQFAEDLQRWDQAKETLKSVLRPNLGHPDSEQALGELCQQEELRQREEAEGIDCNTKQLQACVSECTQHFLMSLASLTERLLLELDETLTVDDVLAADVEIPKEKLSTLIRRKQAGLPLESTEHKPLVERGSRVWPGIGIVDPTDPEGDSARHISASVTTAKTTVGHISAVQARDSTRSRFLQDTEAVLTRIREESRQQHFDAQRWRDWWCQSVRNIKALYMG

Target ORF information:

RefSeq Version	XM_031891402.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891402.1

ORF Insert Sequence:

ATGGCAGCAG TGGGACAGAT CCAGGCAGTT CCTAGTGGAA GAGTCTACAG GCAACTTTTT 
GATGCTGAGG TTCAGCTTGT GCGGTCCCTC GGAGAGGCTC GTGCTAGAGC GGTGCAGCAT 
GGAGGTGCAG TGGACACTGG GAACATTCCT CCAATCAGAA GCCGGGATAA CAACACAGCA 
TTGCCTGAGT CTCACCACCA GAGCACGGAT AAAATGACTC CACCTGCTAG GAAAAAGCAG 
GAAAGTGAGG ATGATATTGC AGCAAGAGAA GTGAGAGGTC TTTCTGATGT CATCGTTCCA 
GAAAAGAAAG ATACCGGCAT TCTTGAGCGG ATCGCTGCAA ACAGACTGGA ACGCCATGAA 
GTGGCACTGT CCAACCTGCA CAGGGAACTG GCTGAGATCA GCAAAGAGAT GGAGCTATCA 
GTATTGGAAG TGGGGAAGCA CCTGCAGCAA AAGATCTCGG AATCAGATGC AAATATTGAA 
CTTTTGTTTC AGAAACTGGA AATCGGTAAC GACAGCACCA CCACAAAGGA AATAAATGAG 
ACTTGGGATG CAGTGACCCA GGAATCTGCC CGTACAAGGC AGTGCATCAA AAGAACCGAA 
AGCACTTTAC TGGCGCTAGA AGAAAACAGA GCGAAACGAA TTTCTGAAGT CTTAAAGAAA 
TACACGTCCC TCTTAAAGGA CATCTGCCAT CTCATGCCCT CCGACGTGGA CAGATTTATT 
CATAATGAAG CCATGATGAT TAACCAGGCA ATTTTAGCTA ACCACCGAGC TCTGGCTAGA 
TTATCTGTAA ACCTTCTGGA AGCCAATCTG AAGAGGGAGA ATACCCAGCG CTTCCAATGG 
CAGGACCTGA AGAAGGCCTG GGAGTGTTAC CAGAGGGAGA TCATAATGAA GGAGTTCAGG 
GTGTTTGCTG AGAAAGAAAT GGAACATATA CCCACAAGTG TGAAAGTATT AGTGGATCAG 
CTGACCACCC AACTCAGCTC TCTCAAAGAA AGGAGACTTC AGATTCTGGG TTTAGCCAGA 
AGTTTTGTAC CTCCTACTTG CACGAGAACA GGTGTCATGG AATGGTATGA ATCGTTGGCA 
GCCCTGAACA GTCAAGCTGA GTCCATCAAC AAACAATTCT TAGAGAAGCT TCAGGAGCTG 
CGGGAGGAAT TCTGCCAAAA ATTTGAGGTG AAAGCAGAAA GCTGTAAAGA GCAGTTGGTC 
AGTGTGGATA TTTGTACCCC AGAGGAAGCT GAGGATATAA TAACCAAAGA ACTCAGCCCA 
GTGACGGCAC AGATCCACAT GAAATTTAAG GAAGAGCAAG AGAAGTGGGA AAAACTGCTG 
GTACATAGCT CTAAGTTAAC GATCCTTCAG ACCCAGAAGT TGCTGAAATT CTTGGAAGAT 
GTTGTTGATA TTTGGCAAAC GCTGGAACGT GGCCTCTCCA AACAGGAAAA TGCTGTGCAG 
CTAAGGATGG AGCAGTGTCG CTATAAATAT GATAGTGACA GCAAGGCCAA GGAGGCAAAT 
TTGGACAAGA TACTGGACAG ACTGAGACAA GACAGCACTA AGGAAACATT ACGTAGCACA 
TTGGGGAAAG CACTGGCGTC CTTGGAAACC ATAAAAGCAG GATACAAGAC TTTTCATTTG 
GACCAAGTCG CTGTAATTGA TTCCTACCCT GCCCTGGTGC TTGCAGAGTT GGCCTCCTAC 
AGCTGCGCTC TTAGCAATTA TTTTGGGGTC AAAGAGGTGT ATGGGCAGGT GCAGAAGAAG 
GATGGGGAGA ACCCCAGCAC CAGTATAACA ACGATTCCGG CAGATGGAGT GAGGTCTCAT 
TCTAGAGAGG GGATATCAGT AAACTCCGAA TTTAATTCCT ACAACTATTC TGCAGTAGAA 
AGTGTTTTGC TGGAAGGTGA ATGGCTGGAA GGGCCAGGAG ATGGAACCCA CAACACAAGA 
GAAGACACGG AAGTGCCTGG AATAGTACAG TGTGATTCTA CTATGGCACC AGTGCAAACC 
CCTTATCGGG AGAGCACCCA AGCAGCAGAG GGAGCTGGTG AGCAGACATC ACAGGGAGCC 
CAGATTCCAT TTGAGCTCTT CACAACATCT AAAGGGAACA CCTATACTGC TTTGCGTTTG 
GCATCAGAAG ATGACACGGC GGTGCCTGAG TTTCCAGGAG AACATTCCGA CACAAATATA 
TTCATGACGG AGGCGGTGCT GGACGATTCC GTTCCCTTAA ATCATGACAT AATGGTCATA 
CCCGAGCAAC AGTTGATTGT TATTAGGAAA AGCGTCCGGC TTGGGTTCTT TGAGCACCTG 
GAGACCTGGT TCGGTGAGAC CCTTTCCAGT GCCCAGGGTA TCGTCAGTGC CAAGAAGGAA 
GAGTTCAATT CTGAGTTAGC GCTTCGCTAT CACCTGCACG AGCCAAGGAG CCAACGGATC 
GAGATGGATA TCCATAACGT CAGAGCTGCC GAGCTGATTC TGCACTCCGA GAGAGTGGAT 
CGTCACTGTG ATGGGGTAAG CCATTCTCTG GAGCAGCTGA AGCGGGAGAG TTCCGTGCTG 
ATTGAGAACA TGAAGCAACA GACTCTCGGA TTCCGCAGCA AGATCCGGGC CATGGAGAGC 
ATGTTCCTCG GTGCCAACAA GTCTGATAAG CTTGTGGCCC TGACACAGTC TCTGCCGTCC 
ATTTTGGACA GCCACGTAAG CGGGGTTCAG ACTTCCATGC GCAACTACCG GCAGCATGTA 
GAGGAAATGC TCGGAACCCT GCGCGACACA AACTCGGACT TCATCAAATC ATTCAGATTG 
TTCTCAGAGG GCGGAAACTT TTCTCCCGAT GAAGTGGAGG TGTTTCGCAA GAAGCTGCAT 
AAAGCGTCAG ACACCATCGC CTCTTTCGAG GGCTCCATCA TGGTGGATTT GGAGGGCTTG 
GAGTCTGTGT GTTTAGAACA GGCCACTGAG ATTGTAAAGA AATTTGAGGA CAAATTCAAC 
ATGTTAACAA CGGATGCAAT TTTCCTAGAA AACATTCAGA AGCTGCTGAC GAACCTGCAA 
GTGAAAATCA AAGCCTTGGT TGTGAGCAGC AATTTTCAGA CTCAGCAGAT CAACTCCTCT 
CTGGAGCAGC TCCAACAGAA GACAGATGCC TGCGCTCATC CCAATATGGA TAAGGAAACA 
GTCACAGCTG AAGAACTGTA CTCCTTTGTG CAAATCCCCA TGGGTCAGCT GGCAAAGAGA 
AGTCTCTATC TGTCCTGCCT TCTAGAGCCC AGCCAAATAC TCTCTGAGAC CCCACTGCAA 
GGCCCTATTG CAACGGCATC ACGTTCGGAC ACCCCACTCC GTCAAGACGC GAAAGGAACC 
TTTACGACCC CCGACAACCT TCTTACTCCG AGCAGAGTGG GGAAGTTGGC TCTCGATGAT 
CCCGCTGTGA GTGTCATCAA GAACATTACC AGAACAAAGC GGGTTATGGA TTCACAACAG 
GACTCTGACC GTGCAAGTAG CTATCAGCGG CCGGGCACAG TCTCACATCA GGCTGCTGCC 
ATAGCGCCAC ATCCACCATC GCAGTCGGGT AAACCTCATC TGAAGAAAAA GAAACCCCCA 
TCAAGCAATG GTGAAAATCC TGGCCAGTCT GTTCAGGCTC CAAGTGTCCG GAAGCTTACA 
AAGCCCAGCA GGTTTGACAA GAAGTACCAA TTGTTTGGGG AGAACAGAGT CGAATCAGAC 
AACTTCAAGG GAATTCTCAC CTGTATACTG TGGGAGAGCA ATGACACCTT ACTGTACCTG 
GCTGAGGAGT TCTATAAGAA GAAGGACAGG CGGAACATTG GCCGGACAGA TCTTCTTCAA 
GAGACGTTTG AGGAGTGTGC AGATACGCTG GTGTTTAAAC TGCAGTCCTA CGAGAAGCAG 
GCCCTGGAGT ATCAGAACAA CTGCCTGCTG GAGTTCCGGG AGCAGCTACA AACTTTCGAG 
AGGCTGGTAA ATGAGGTGCC GCCGCTGGTC ATAGAGAGTC ACCGGAGGCA ACACCTGGAC 
AAACTGCTGG CGGCCACTGA CCAGATACGG AAGCAGTTTG CTGAGGACCT GCAGCGCTGG 
GACCAAGCCA AGGAGACACT GAAGAGTGTG CTGAGGCCAA ATTTAGGCCA CCCAGACAGC 
GAGCAGGCCC TGGGGGAGCT GTGCCAGCAG GAGGAACTAA GGCAGAGAGA GGAGGCAGAA 
GGGATTGACT GCAACACTAA ACAATTACAA GCGTGCGTCT CCGAGTGCAC CCAGCACTTC 
CTGATGTCTC TGGCCTCCCT CACCGAGAGA CTGCTGCTGG AACTCGATGA GACACTTACA 
GTCGATGATG TTTTAGCAGC TGATGTTGAG ATTCCCAAAG AAAAACTGTC AACGCTGATC 
AGACGCAAGC AGGCGGGGCT GCCGTTGGAA AGCACAGAGC ACAAGCCACT GGTGGAGAGA 
GGCAGCAGGG TCTGGCCAGG AATTGGAATT GTGGATCCTA CAGACCCTGA AGGGGACAGT 
GCGCGCCACA TCAGCGCATC GGTCACCACG GCCAAGACCA CAGTCGGCCA CATCTCAGCG 
GTGCAGGCTC GCGACTCCAC TCGCTCGCGG TTCCTGCAGG ACACAGAGGC GGTACTGACC 
CGTATCCGGG AGGAGAGCCG GCAGCAGCAC TTTGATGCCC AGCGCTGGAG GGATTGGTGG 
TGCCAGTCTG TACGCAATAT AAAAGCTCTG TATATGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccdc180 ( XM_012968714.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824168.2 Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa64701
Clone ID Related Accession (Same CDS sequence)	XM_031891402.1 , XM_012968714.3
Accession Version	XM_012968714.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	coiled-coil domain-containing protein 180 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968714.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG TGGGACAGAT CCAGGCAGTT CCTAGTGGAA GAGTCTACAG GCAACTTTTT 
GATGCTGAGG TTCAGCTTGT GCGGTCCCTC GGAGAGGCTC GTGCTAGAGC GGTGCAGCAT 
GGAGGTGCAG TGGACACTGG GAACATTCCT CCAATCAGAA GCCGGGATAA CAACACAGCA 
TTGCCTGAGT CTCACCACCA GAGCACGGAT AAAATGACTC CACCTGCTAG GAAAAAGCAG 
GAAAGTGAGG ATGATATTGC AGCAAGAGAA GTGAGAGGTC TTTCTGATGT CATCGTTCCA 
GAAAAGAAAG ATACCGGCAT TCTTGAGCGG ATCGCTGCAA ACAGACTGGA ACGCCATGAA 
GTGGCACTGT CCAACCTGCA CAGGGAACTG GCTGAGATCA GCAAAGAGAT GGAGCTATCA 
GTATTGGAAG TGGGGAAGCA CCTGCAGCAA AAGATCTCGG AATCAGATGC AAATATTGAA 
CTTTTGTTTC AGAAACTGGA AATCGGTAAC GACAGCACCA CCACAAAGGA AATAAATGAG 
ACTTGGGATG CAGTGACCCA GGAATCTGCC CGTACAAGGC AGTGCATCAA AAGAACCGAA 
AGCACTTTAC TGGCGCTAGA AGAAAACAGA GCGAAACGAA TTTCTGAAGT CTTAAAGAAA 
TACACGTCCC TCTTAAAGGA CATCTGCCAT CTCATGCCCT CCGACGTGGA CAGATTTATT 
CATAATGAAG CCATGATGAT TAACCAGGCA ATTTTAGCTA ACCACCGAGC TCTGGCTAGA 
TTATCTGTAA ACCTTCTGGA AGCCAATCTG AAGAGGGAGA ATACCCAGCG CTTCCAATGG 
CAGGACCTGA AGAAGGCCTG GGAGTGTTAC CAGAGGGAGA TCATAATGAA GGAGTTCAGG 
GTGTTTGCTG AGAAAGAAAT GGAACATATA CCCACAAGTG TGAAAGTATT AGTGGATCAG 
CTGACCACCC AACTCAGCTC TCTCAAAGAA AGGAGACTTC AGATTCTGGG TTTAGCCAGA 
AGTTTTGTAC CTCCTACTTG CACGAGAACA GGTGTCATGG AATGGTATGA ATCGTTGGCA 
GCCCTGAACA GTCAAGCTGA GTCCATCAAC AAACAATTCT TAGAGAAGCT TCAGGAGCTG 
CGGGAGGAAT TCTGCCAAAA ATTTGAGGTG AAAGCAGAAA GCTGTAAAGA GCAGTTGGTC 
AGTGTGGATA TTTGTACCCC AGAGGAAGCT GAGGATATAA TAACCAAAGA ACTCAGCCCA 
GTGACGGCAC AGATCCACAT GAAATTTAAG GAAGAGCAAG AGAAGTGGGA AAAACTGCTG 
GTACATAGCT CTAAGTTAAC GATCCTTCAG ACCCAGAAGT TGCTGAAATT CTTGGAAGAT 
GTTGTTGATA TTTGGCAAAC GCTGGAACGT GGCCTCTCCA AACAGGAAAA TGCTGTGCAG 
CTAAGGATGG AGCAGTGTCG CTATAAATAT GATAGTGACA GCAAGGCCAA GGAGGCAAAT 
TTGGACAAGA TACTGGACAG ACTGAGACAA GACAGCACTA AGGAAACATT ACGTAGCACA 
TTGGGGAAAG CACTGGCGTC CTTGGAAACC ATAAAAGCAG GATACAAGAC TTTTCATTTG 
GACCAAGTCG CTGTAATTGA TTCCTACCCT GCCCTGGTGC TTGCAGAGTT GGCCTCCTAC 
AGCTGCGCTC TTAGCAATTA TTTTGGGGTC AAAGAGGTGT ATGGGCAGGT GCAGAAGAAG 
GATGGGGAGA ACCCCAGCAC CAGTATAACA ACGATTCCGG CAGATGGAGT GAGGTCTCAT 
TCTAGAGAGG GGATATCAGT AAACTCCGAA TTTAATTCCT ACAACTATTC TGCAGTAGAA 
AGTGTTTTGC TGGAAGGTGA ATGGCTGGAA GGGCCAGGAG ATGGAACCCA CAACACAAGA 
GAAGACACGG AAGTGCCTGG AATAGTACAG TGTGATTCTA CTATGGCACC AGTGCAAACC 
CCTTATCGGG AGAGCACCCA AGCAGCAGAG GGAGCTGGTG AGCAGACATC ACAGGGAGCC 
CAGATTCCAT TTGAGCTCTT CACAACATCT AAAGGGAACA CCTATACTGC TTTGCGTTTG 
GCATCAGAAG ATGACACGGC GGTGCCTGAG TTTCCAGGAG AACATTCCGA CACAAATATA 
TTCATGACGG AGGCGGTGCT GGACGATTCC GTTCCCTTAA ATCATGACAT AATGGTCATA 
CCCGAGCAAC AGTTGATTGT TATTAGGAAA AGCGTCCGGC TTGGGTTCTT TGAGCACCTG 
GAGACCTGGT TCGGTGAGAC CCTTTCCAGT GCCCAGGGTA TCGTCAGTGC CAAGAAGGAA 
GAGTTCAATT CTGAGTTAGC GCTTCGCTAT CACCTGCACG AGCCAAGGAG CCAACGGATC 
GAGATGGATA TCCATAACGT CAGAGCTGCC GAGCTGATTC TGCACTCCGA GAGAGTGGAT 
CGTCACTGTG ATGGGGTAAG CCATTCTCTG GAGCAGCTGA AGCGGGAGAG TTCCGTGCTG 
ATTGAGAACA TGAAGCAACA GACTCTCGGA TTCCGCAGCA AGATCCGGGC CATGGAGAGC 
ATGTTCCTCG GTGCCAACAA GTCTGATAAG CTTGTGGCCC TGACACAGTC TCTGCCGTCC 
ATTTTGGACA GCCACGTAAG CGGGGTTCAG ACTTCCATGC GCAACTACCG GCAGCATGTA 
GAGGAAATGC TCGGAACCCT GCGCGACACA AACTCGGACT TCATCAAATC ATTCAGATTG 
TTCTCAGAGG GCGGAAACTT TTCTCCCGAT GAAGTGGAGG TGTTTCGCAA GAAGCTGCAT 
AAAGCGTCAG ACACCATCGC CTCTTTCGAG GGCTCCATCA TGGTGGATTT GGAGGGCTTG 
GAGTCTGTGT GTTTAGAACA GGCCACTGAG ATTGTAAAGA AATTTGAGGA CAAATTCAAC 
ATGTTAACAA CGGATGCAAT TTTCCTAGAA AACATTCAGA AGCTGCTGAC GAACCTGCAA 
GTGAAAATCA AAGCCTTGGT TGTGAGCAGC AATTTTCAGA CTCAGCAGAT CAACTCCTCT 
CTGGAGCAGC TCCAACAGAA GACAGATGCC TGCGCTCATC CCAATATGGA TAAGGAAACA 
GTCACAGCTG AAGAACTGTA CTCCTTTGTG CAAATCCCCA TGGGTCAGCT GGCAAAGAGA 
AGTCTCTATC TGTCCTGCCT TCTAGAGCCC AGCCAAATAC TCTCTGAGAC CCCACTGCAA 
GGCCCTATTG CAACGGCATC ACGTTCGGAC ACCCCACTCC GTCAAGACGC GAAAGGAACC 
TTTACGACCC CCGACAACCT TCTTACTCCG AGCAGAGTGG GGAAGTTGGC TCTCGATGAT 
CCCGCTGTGA GTGTCATCAA GAACATTACC AGAACAAAGC GGGTTATGGA TTCACAACAG 
GACTCTGACC GTGCAAGTAG CTATCAGCGG CCGGGCACAG TCTCACATCA GGCTGCTGCC 
ATAGCGCCAC ATCCACCATC GCAGTCGGGT AAACCTCATC TGAAGAAAAA GAAACCCCCA 
TCAAGCAATG GTGAAAATCC TGGCCAGTCT GTTCAGGCTC CAAGTGTCCG GAAGCTTACA 
AAGCCCAGCA GGTTTGACAA GAAGTACCAA TTGTTTGGGG AGAACAGAGT CGAATCAGAC 
AACTTCAAGG GAATTCTCAC CTGTATACTG TGGGAGAGCA ATGACACCTT ACTGTACCTG 
GCTGAGGAGT TCTATAAGAA GAAGGACAGG CGGAACATTG GCCGGACAGA TCTTCTTCAA 
GAGACGTTTG AGGAGTGTGC AGATACGCTG GTGTTTAAAC TGCAGTCCTA CGAGAAGCAG 
GCCCTGGAGT ATCAGAACAA CTGCCTGCTG GAGTTCCGGG AGCAGCTACA AACTTTCGAG 
AGGCTGGTAA ATGAGGTGCC GCCGCTGGTC ATAGAGAGTC ACCGGAGGCA ACACCTGGAC 
AAACTGCTGG CGGCCACTGA CCAGATACGG AAGCAGTTTG CTGAGGACCT GCAGCGCTGG 
GACCAAGCCA AGGAGACACT GAAGAGTGTG CTGAGGCCAA ATTTAGGCCA CCCAGACAGC 
GAGCAGGCCC TGGGGGAGCT GTGCCAGCAG GAGGAACTAA GGCAGAGAGA GGAGGCAGAA 
GGGATTGACT GCAACACTAA ACAATTACAA GCGTGCGTCT CCGAGTGCAC CCAGCACTTC 
CTGATGTCTC TGGCCTCCCT CACCGAGAGA CTGCTGCTGG AACTCGATGA GACACTTACA 
GTCGATGATG TTTTAGCAGC TGATGTTGAG ATTCCCAAAG AAAAACTGTC AACGCTGATC 
AGACGCAAGC AGGCGGGGCT GCCGTTGGAA AGCACAGAGC ACAAGCCACT GGTGGAGAGA 
GGCAGCAGGG TCTGGCCAGG AATTGGAATT GTGGATCCTA CAGACCCTGA AGGGGACAGT 
GCGCGCCACA TCAGCGCATC GGTCACCACG GCCAAGACCA CAGTCGGCCA CATCTCAGCG 
GTGCAGGCTC GCGACTCCAC TCGCTCGCGG TTCCTGCAGG ACACAGAGGC GGTACTGACC 
CGTATCCGGG AGGAGAGCCG GCAGCAGCAC TTTGATGCCC AGCGCTGGAG GGATTGGTGG 
TGCCAGTCTG TACGCAATAT AAAAGCTCTG TATATGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824168.2
CDS	180..4841
Translation	MAAVGQIQAVPSGRVYRQLFDAEVQLVRSLGEARARAVQHGGAVDTGNIPPIRSRDNNTALPESHHQSTDKMTPPARKKQESEDDIAAREVRGLSDVIVPEKKDTGILERIAANRLERHEVALSNLHRELAEISKEMELSVLEVGKHLQQKISESDANIELLFQKLEIGNDSTTTKEINETWDAVTQESARTRQCIKRTESTLLALEENRAKRISEVLKKYTSLLKDICHLMPSDVDRFIHNEAMMINQAILANHRALARLSVNLLEANLKRENTQRFQWQDLKKAWECYQREIIMKEFRVFAEKEMEHIPTSVKVLVDQLTTQLSSLKERRLQILGLARSFVPPTCTRTGVMEWYESLAALNSQAESINKQFLEKLQELREEFCQKFEVKAESCKEQLVSVDICTPEEAEDIITKELSPVTAQIHMKFKEEQEKWEKLLVHSSKLTILQTQKLLKFLEDVVDIWQTLERGLSKQENAVQLRMEQCRYKYDSDSKAKEANLDKILDRLRQDSTKETLRSTLGKALASLETIKAGYKTFHLDQVAVIDSYPALVLAELASYSCALSNYFGVKEVYGQVQKKDGENPSTSITTIPADGVRSHSREGISVNSEFNSYNYSAVESVLLEGEWLEGPGDGTHNTREDTEVPGIVQCDSTMAPVQTPYRESTQAAEGAGEQTSQGAQIPFELFTTSKGNTYTALRLASEDDTAVPEFPGEHSDTNIFMTEAVLDDSVPLNHDIMVIPEQQLIVIRKSVRLGFFEHLETWFGETLSSAQGIVSAKKEEFNSELALRYHLHEPRSQRIEMDIHNVRAAELILHSERVDRHCDGVSHSLEQLKRESSVLIENMKQQTLGFRSKIRAMESMFLGANKSDKLVALTQSLPSILDSHVSGVQTSMRNYRQHVEEMLGTLRDTNSDFIKSFRLFSEGGNFSPDEVEVFRKKLHKASDTIASFEGSIMVDLEGLESVCLEQATEIVKKFEDKFNMLTTDAIFLENIQKLLTNLQVKIKALVVSSNFQTQQINSSLEQLQQKTDACAHPNMDKETVTAEELYSFVQIPMGQLAKRSLYLSCLLEPSQILSETPLQGPIATASRSDTPLRQDAKGTFTTPDNLLTPSRVGKLALDDPAVSVIKNITRTKRVMDSQQDSDRASSYQRPGTVSHQAAAIAPHPPSQSGKPHLKKKKPPSSNGENPGQSVQAPSVRKLTKPSRFDKKYQLFGENRVESDNFKGILTCILWESNDTLLYLAEEFYKKKDRRNIGRTDLLQETFEECADTLVFKLQSYEKQALEYQNNCLLEFREQLQTFERLVNEVPPLVIESHRRQHLDKLLAATDQIRKQFAEDLQRWDQAKETLKSVLRPNLGHPDSEQALGELCQQEELRQREEAEGIDCNTKQLQACVSECTQHFLMSLASLTERLLLELDETLTVDDVLAADVEIPKEKLSTLIRRKQAGLPLESTEHKPLVERGSRVWPGIGIVDPTDPEGDSARHISASVTTAKTTVGHISAVQARDSTRSRFLQDTEAVLTRIREESRQQHFDAQRWRDWWCQSVRNIKALYMG

Target ORF information:

RefSeq Version	XM_012968714.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968714.3

ORF Insert Sequence:

ATGGCAGCAG TGGGACAGAT CCAGGCAGTT CCTAGTGGAA GAGTCTACAG GCAACTTTTT 
GATGCTGAGG TTCAGCTTGT GCGGTCCCTC GGAGAGGCTC GTGCTAGAGC GGTGCAGCAT 
GGAGGTGCAG TGGACACTGG GAACATTCCT CCAATCAGAA GCCGGGATAA CAACACAGCA 
TTGCCTGAGT CTCACCACCA GAGCACGGAT AAAATGACTC CACCTGCTAG GAAAAAGCAG 
GAAAGTGAGG ATGATATTGC AGCAAGAGAA GTGAGAGGTC TTTCTGATGT CATCGTTCCA 
GAAAAGAAAG ATACCGGCAT TCTTGAGCGG ATCGCTGCAA ACAGACTGGA ACGCCATGAA 
GTGGCACTGT CCAACCTGCA CAGGGAACTG GCTGAGATCA GCAAAGAGAT GGAGCTATCA 
GTATTGGAAG TGGGGAAGCA CCTGCAGCAA AAGATCTCGG AATCAGATGC AAATATTGAA 
CTTTTGTTTC AGAAACTGGA AATCGGTAAC GACAGCACCA CCACAAAGGA AATAAATGAG 
ACTTGGGATG CAGTGACCCA GGAATCTGCC CGTACAAGGC AGTGCATCAA AAGAACCGAA 
AGCACTTTAC TGGCGCTAGA AGAAAACAGA GCGAAACGAA TTTCTGAAGT CTTAAAGAAA 
TACACGTCCC TCTTAAAGGA CATCTGCCAT CTCATGCCCT CCGACGTGGA CAGATTTATT 
CATAATGAAG CCATGATGAT TAACCAGGCA ATTTTAGCTA ACCACCGAGC TCTGGCTAGA 
TTATCTGTAA ACCTTCTGGA AGCCAATCTG AAGAGGGAGA ATACCCAGCG CTTCCAATGG 
CAGGACCTGA AGAAGGCCTG GGAGTGTTAC CAGAGGGAGA TCATAATGAA GGAGTTCAGG 
GTGTTTGCTG AGAAAGAAAT GGAACATATA CCCACAAGTG TGAAAGTATT AGTGGATCAG 
CTGACCACCC AACTCAGCTC TCTCAAAGAA AGGAGACTTC AGATTCTGGG TTTAGCCAGA 
AGTTTTGTAC CTCCTACTTG CACGAGAACA GGTGTCATGG AATGGTATGA ATCGTTGGCA 
GCCCTGAACA GTCAAGCTGA GTCCATCAAC AAACAATTCT TAGAGAAGCT TCAGGAGCTG 
CGGGAGGAAT TCTGCCAAAA ATTTGAGGTG AAAGCAGAAA GCTGTAAAGA GCAGTTGGTC 
AGTGTGGATA TTTGTACCCC AGAGGAAGCT GAGGATATAA TAACCAAAGA ACTCAGCCCA 
GTGACGGCAC AGATCCACAT GAAATTTAAG GAAGAGCAAG AGAAGTGGGA AAAACTGCTG 
GTACATAGCT CTAAGTTAAC GATCCTTCAG ACCCAGAAGT TGCTGAAATT CTTGGAAGAT 
GTTGTTGATA TTTGGCAAAC GCTGGAACGT GGCCTCTCCA AACAGGAAAA TGCTGTGCAG 
CTAAGGATGG AGCAGTGTCG CTATAAATAT GATAGTGACA GCAAGGCCAA GGAGGCAAAT 
TTGGACAAGA TACTGGACAG ACTGAGACAA GACAGCACTA AGGAAACATT ACGTAGCACA 
TTGGGGAAAG CACTGGCGTC CTTGGAAACC ATAAAAGCAG GATACAAGAC TTTTCATTTG 
GACCAAGTCG CTGTAATTGA TTCCTACCCT GCCCTGGTGC TTGCAGAGTT GGCCTCCTAC 
AGCTGCGCTC TTAGCAATTA TTTTGGGGTC AAAGAGGTGT ATGGGCAGGT GCAGAAGAAG 
GATGGGGAGA ACCCCAGCAC CAGTATAACA ACGATTCCGG CAGATGGAGT GAGGTCTCAT 
TCTAGAGAGG GGATATCAGT AAACTCCGAA TTTAATTCCT ACAACTATTC TGCAGTAGAA 
AGTGTTTTGC TGGAAGGTGA ATGGCTGGAA GGGCCAGGAG ATGGAACCCA CAACACAAGA 
GAAGACACGG AAGTGCCTGG AATAGTACAG TGTGATTCTA CTATGGCACC AGTGCAAACC 
CCTTATCGGG AGAGCACCCA AGCAGCAGAG GGAGCTGGTG AGCAGACATC ACAGGGAGCC 
CAGATTCCAT TTGAGCTCTT CACAACATCT AAAGGGAACA CCTATACTGC TTTGCGTTTG 
GCATCAGAAG ATGACACGGC GGTGCCTGAG TTTCCAGGAG AACATTCCGA CACAAATATA 
TTCATGACGG AGGCGGTGCT GGACGATTCC GTTCCCTTAA ATCATGACAT AATGGTCATA 
CCCGAGCAAC AGTTGATTGT TATTAGGAAA AGCGTCCGGC TTGGGTTCTT TGAGCACCTG 
GAGACCTGGT TCGGTGAGAC CCTTTCCAGT GCCCAGGGTA TCGTCAGTGC CAAGAAGGAA 
GAGTTCAATT CTGAGTTAGC GCTTCGCTAT CACCTGCACG AGCCAAGGAG CCAACGGATC 
GAGATGGATA TCCATAACGT CAGAGCTGCC GAGCTGATTC TGCACTCCGA GAGAGTGGAT 
CGTCACTGTG ATGGGGTAAG CCATTCTCTG GAGCAGCTGA AGCGGGAGAG TTCCGTGCTG 
ATTGAGAACA TGAAGCAACA GACTCTCGGA TTCCGCAGCA AGATCCGGGC CATGGAGAGC 
ATGTTCCTCG GTGCCAACAA GTCTGATAAG CTTGTGGCCC TGACACAGTC TCTGCCGTCC 
ATTTTGGACA GCCACGTAAG CGGGGTTCAG ACTTCCATGC GCAACTACCG GCAGCATGTA 
GAGGAAATGC TCGGAACCCT GCGCGACACA AACTCGGACT TCATCAAATC ATTCAGATTG 
TTCTCAGAGG GCGGAAACTT TTCTCCCGAT GAAGTGGAGG TGTTTCGCAA GAAGCTGCAT 
AAAGCGTCAG ACACCATCGC CTCTTTCGAG GGCTCCATCA TGGTGGATTT GGAGGGCTTG 
GAGTCTGTGT GTTTAGAACA GGCCACTGAG ATTGTAAAGA AATTTGAGGA CAAATTCAAC 
ATGTTAACAA CGGATGCAAT TTTCCTAGAA AACATTCAGA AGCTGCTGAC GAACCTGCAA 
GTGAAAATCA AAGCCTTGGT TGTGAGCAGC AATTTTCAGA CTCAGCAGAT CAACTCCTCT 
CTGGAGCAGC TCCAACAGAA GACAGATGCC TGCGCTCATC CCAATATGGA TAAGGAAACA 
GTCACAGCTG AAGAACTGTA CTCCTTTGTG CAAATCCCCA TGGGTCAGCT GGCAAAGAGA 
AGTCTCTATC TGTCCTGCCT TCTAGAGCCC AGCCAAATAC TCTCTGAGAC CCCACTGCAA 
GGCCCTATTG CAACGGCATC ACGTTCGGAC ACCCCACTCC GTCAAGACGC GAAAGGAACC 
TTTACGACCC CCGACAACCT TCTTACTCCG AGCAGAGTGG GGAAGTTGGC TCTCGATGAT 
CCCGCTGTGA GTGTCATCAA GAACATTACC AGAACAAAGC GGGTTATGGA TTCACAACAG 
GACTCTGACC GTGCAAGTAG CTATCAGCGG CCGGGCACAG TCTCACATCA GGCTGCTGCC 
ATAGCGCCAC ATCCACCATC GCAGTCGGGT AAACCTCATC TGAAGAAAAA GAAACCCCCA 
TCAAGCAATG GTGAAAATCC TGGCCAGTCT GTTCAGGCTC CAAGTGTCCG GAAGCTTACA 
AAGCCCAGCA GGTTTGACAA GAAGTACCAA TTGTTTGGGG AGAACAGAGT CGAATCAGAC 
AACTTCAAGG GAATTCTCAC CTGTATACTG TGGGAGAGCA ATGACACCTT ACTGTACCTG 
GCTGAGGAGT TCTATAAGAA GAAGGACAGG CGGAACATTG GCCGGACAGA TCTTCTTCAA 
GAGACGTTTG AGGAGTGTGC AGATACGCTG GTGTTTAAAC TGCAGTCCTA CGAGAAGCAG 
GCCCTGGAGT ATCAGAACAA CTGCCTGCTG GAGTTCCGGG AGCAGCTACA AACTTTCGAG 
AGGCTGGTAA ATGAGGTGCC GCCGCTGGTC ATAGAGAGTC ACCGGAGGCA ACACCTGGAC 
AAACTGCTGG CGGCCACTGA CCAGATACGG AAGCAGTTTG CTGAGGACCT GCAGCGCTGG 
GACCAAGCCA AGGAGACACT GAAGAGTGTG CTGAGGCCAA ATTTAGGCCA CCCAGACAGC 
GAGCAGGCCC TGGGGGAGCT GTGCCAGCAG GAGGAACTAA GGCAGAGAGA GGAGGCAGAA 
GGGATTGACT GCAACACTAA ACAATTACAA GCGTGCGTCT CCGAGTGCAC CCAGCACTTC 
CTGATGTCTC TGGCCTCCCT CACCGAGAGA CTGCTGCTGG AACTCGATGA GACACTTACA 
GTCGATGATG TTTTAGCAGC TGATGTTGAG ATTCCCAAAG AAAAACTGTC AACGCTGATC 
AGACGCAAGC AGGCGGGGCT GCCGTTGGAA AGCACAGAGC ACAAGCCACT GGTGGAGAGA 
GGCAGCAGGG TCTGGCCAGG AATTGGAATT GTGGATCCTA CAGACCCTGA AGGGGACAGT 
GCGCGCCACA TCAGCGCATC GGTCACCACG GCCAAGACCA CAGTCGGCCA CATCTCAGCG 
GTGCAGGCTC GCGACTCCAC TCGCTCGCGG TTCCTGCAGG ACACAGAGGC GGTACTGACC 
CGTATCCGGG AGGAGAGCCG GCAGCAGCAC TTTGATGCCC AGCGCTGGAG GGATTGGTGG 
TGCCAGTCTG TACGCAATAT AAAAGCTCTG TATATGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccdc180 ( XM_031891403.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747263.1 Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X3, mRNA.

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CloneID	OXa64702
Clone ID Related Accession (Same CDS sequence)	XM_031891403.1
Accession Version	XM_031891403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	coiled-coil domain-containing protein 180 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG TGGGACAGAT CCAGGCAGTT CCTAGTGGAA GAGTCTACAG GCAACTTTTT 
GATGCTGAGG TTCAGCTTGT GCGGTCCCTC GGAGAGGCTC GTGCTAGAGC GGTGCAGCAT 
GGAGGTGCAG TGGACACTGG GAACATTCCT CCAATCAGAA GCCGGGATAA CAACACAGCA 
TTGCCTGAGT CTCACCACCA GAGCACGGAT AAAATGACTC CACCTGCTAG GAAAAAGCAG 
GAAAGTGAGG ATGATATTGC AGCAAGAGAA GTGAGAGGTC TTTCTGATGT CATCGTTCCA 
GAAAAGAAAG ATACCGGCAT TCTTGAGCGG ATCGCTGCAA ACAGACTGGA ACGCCATGAA 
GTGGCACTGT CCAACCTGCA CAGGGAACTG GCTGAGATCA GCAAAGAGAT GGAGCTATCA 
GTATTGGAAG TGGGGAAGCA CCTGCAGCAA AAGATCTCGG AATCAGATGC AAATATTGAA 
CTTTTGTTTC AGAAACTGGA AATCGGTAAC GACAGCACCA CCACAAAGGA AATAAATGAG 
ACTTGGGATG CAGTGACCCA GGAATCTGCC CGTACAAGGC AGTGCATCAA AAGAACCGAA 
AGCACTTTAC TGGCGCTAGA AGAAAACAGA GCGAAACGAA TTTCTGAAGT CTTAAAGAAA 
TACACGTCCC TCTTAAAGGA CATCTGCCAT CTCATGCCCT CCGACGTGGA CAGATTTATT 
CATAATGAAG CCATGATGAT TAACCAGGCA ATTTTAGCTA ACCACCGAGC TCTGGCTAGA 
TTATCTGTAA ACCTTCTGGA AGCCAATCTG AAGAGGGAGA ATACCCAGCG CTTCCAATGG 
CAGGACCTGA AGAAGGCCTG GGAGTGTTAC CAGAGGGAGA TCATAATGAA GGAGTTCAGG 
GTGTTTGCTG AGAAAGAAAT GGAACATATA CCCACAAGTG TGAAAGTATT AGTGGATCAG 
CTGACCACCC AACTCAGCTC TCTCAAAGAA AGGAGACTTC AGATTCTGGG TTTAGCCAGT 
TTTGTACCTC CTACTTGCAC GAGAACAGGT GTCATGGAAT GGTATGAATC GTTGGCAGCC 
CTGAACAGTC AAGCTGAGTC CATCAACAAA CAATTCTTAG AGAAGCTTCA GGAGCTGCGG 
GAGGAATTCT GCCAAAAATT TGAGGTGAAA GCAGAAAGCT GTAAAGAGCA GTTGGTCAGT 
GTGGATATTT GTACCCCAGA GGAAGCTGAG GATATAATAA CCAAAGAACT CAGCCCAGTG 
ACGGCACAGA TCCACATGAA ATTTAAGGAA GAGCAAGAGA AGTGGGAAAA ACTGCTGGTA 
CATAGCTCTA AGTTAACGAT CCTTCAGACC CAGAAGTTGC TGAAATTCTT GGAAGATGTT 
GTTGATATTT GGCAAACGCT GGAACGTGGC CTCTCCAAAC AGGAAAATGC TGTGCAGCTA 
AGGATGGAGC AGTGTCGCTA TAAATATGAT AGTGACAGCA AGGCCAAGGA GGCAAATTTG 
GACAAGATAC TGGACAGACT GAGACAAGAC AGCACTAAGG AAACATTACG TAGCACATTG 
GGGAAAGCAC TGGCGTCCTT GGAAACCATA AAAGCAGGAT ACAAGACTTT TCATTTGGAC 
CAAGTCGCTG TAATTGATTC CTACCCTGCC CTGGTGCTTG CAGAGTTGGC CTCCTACAGC 
TGCGCTCTTA GCAATTATTT TGGGGTCAAA GAGGTGTATG GGCAGGTGCA GAAGAAGGAT 
GGGGAGAACC CCAGCACCAG TATAACAACG ATTCCGGCAG ATGGAGTGAG GTCTCATTCT 
AGAGAGGGGA TATCAGTAAA CTCCGAATTT AATTCCTACA ACTATTCTGC AGTAGAAAGT 
GTTTTGCTGG AAGGTGAATG GCTGGAAGGG CCAGGAGATG GAACCCACAA CACAAGAGAA 
GACACGGAAG TGCCTGGAAT AGTACAGTGT GATTCTACTA TGGCACCAGT GCAAACCCCT 
TATCGGGAGA GCACCCAAGC AGCAGAGGGA GCTGGTGAGC AGACATCACA GGGAGCCCAG 
ATTCCATTTG AGCTCTTCAC AACATCTAAA GGGAACACCT ATACTGCTTT GCGTTTGGCA 
TCAGAAGATG ACACGGCGGT GCCTGAGTTT CCAGGAGAAC ATTCCGACAC AAATATATTC 
ATGACGGAGG CGGTGCTGGA CGATTCCGTT CCCTTAAATC ATGACATAAT GGTCATACCC 
GAGCAACAGT TGATTGTTAT TAGGAAAAGC GTCCGGCTTG GGTTCTTTGA GCACCTGGAG 
ACCTGGTTCG GTGAGACCCT TTCCAGTGCC CAGGGTATCG TCAGTGCCAA GAAGGAAGAG 
TTCAATTCTG AGTTAGCGCT TCGCTATCAC CTGCACGAGC CAAGGAGCCA ACGGATCGAG 
ATGGATATCC ATAACGTCAG AGCTGCCGAG CTGATTCTGC ACTCCGAGAG AGTGGATCGT 
CACTGTGATG GGGTAAGCCA TTCTCTGGAG CAGCTGAAGC GGGAGAGTTC CGTGCTGATT 
GAGAACATGA AGCAACAGAC TCTCGGATTC CGCAGCAAGA TCCGGGCCAT GGAGAGCATG 
TTCCTCGGTG CCAACAAGTC TGATAAGCTT GTGGCCCTGA CACAGTCTCT GCCGTCCATT 
TTGGACAGCC ACGTAAGCGG GGTTCAGACT TCCATGCGCA ACTACCGGCA GCATGTAGAG 
GAAATGCTCG GAACCCTGCG CGACACAAAC TCGGACTTCA TCAAATCATT CAGATTGTTC 
TCAGAGGGCG GAAACTTTTC TCCCGATGAA GTGGAGGTGT TTCGCAAGAA GCTGCATAAA 
GCGTCAGACA CCATCGCCTC TTTCGAGGGC TCCATCATGG TGGATTTGGA GGGCTTGGAG 
TCTGTGTGTT TAGAACAGGC CACTGAGATT GTAAAGAAAT TTGAGGACAA ATTCAACATG 
TTAACAACGG ATGCAATTTT CCTAGAAAAC ATTCAGAAGC TGCTGACGAA CCTGCAAGTG 
AAAATCAAAG CCTTGGTTGT GAGCAGCAAT TTTCAGACTC AGCAGATCAA CTCCTCTCTG 
GAGCAGCTCC AACAGAAGAC AGATGCCTGC GCTCATCCCA ATATGGATAA GGAAACAGTC 
ACAGCTGAAG AACTGTACTC CTTTGTGCAA ATCCCCATGG GTCAGCTGGC AAAGAGAAGT 
CTCTATCTGT CCTGCCTTCT AGAGCCCAGC CAAATACTCT CTGAGACCCC ACTGCAAGGC 
CCTATTGCAA CGGCATCACG TTCGGACACC CCACTCCGTC AAGACGCGAA AGGAACCTTT 
ACGACCCCCG ACAACCTTCT TACTCCGAGC AGAGTGGGGA AGTTGGCTCT CGATGATCCC 
GCTGTGAGTG TCATCAAGAA CATTACCAGA ACAAAGCGGG TTATGGATTC ACAACAGGAC 
TCTGACCGTG CAAGTAGCTA TCAGCGGCCG GGCACAGTCT CACATCAGGC TGCTGCCATA 
GCGCCACATC CACCATCGCA GTCGGGTAAA CCTCATCTGA AGAAAAAGAA ACCCCCATCA 
AGCAATGGTG AAAATCCTGG CCAGTCTGTT CAGGCTCCAA GTGTCCGGAA GCTTACAAAG 
CCCAGCAGGT TTGACAAGAA GTACCAATTG TTTGGGGAGA ACAGAGTCGA ATCAGACAAC 
TTCAAGGGAA TTCTCACCTG TATACTGTGG GAGAGCAATG ACACCTTACT GTACCTGGCT 
GAGGAGTTCT ATAAGAAGAA GGACAGGCGG AACATTGGCC GGACAGATCT TCTTCAAGAG 
ACGTTTGAGG AGTGTGCAGA TACGCTGGTG TTTAAACTGC AGTCCTACGA GAAGCAGGCC 
CTGGAGTATC AGAACAACTG CCTGCTGGAG TTCCGGGAGC AGCTACAAAC TTTCGAGAGG 
CTGGTAAATG AGGTGCCGCC GCTGGTCATA GAGAGTCACC GGAGGCAACA CCTGGACAAA 
CTGCTGGCGG CCACTGACCA GATACGGAAG CAGTTTGCTG AGGACCTGCA GCGCTGGGAC 
CAAGCCAAGG AGACACTGAA GAGTGTGCTG AGGCCAAATT TAGGCCACCC AGACAGCGAG 
CAGGCCCTGG GGGAGCTGTG CCAGCAGGAG GAACTAAGGC AGAGAGAGGA GGCAGAAGGG 
ATTGACTGCA ACACTAAACA ATTACAAGCG TGCGTCTCCG AGTGCACCCA GCACTTCCTG 
ATGTCTCTGG CCTCCCTCAC CGAGAGACTG CTGCTGGAAC TCGATGAGAC ACTTACAGTC 
GATGATGTTT TAGCAGCTGA TGTTGAGATT CCCAAAGAAA AACTGTCAAC GCTGATCAGA 
CGCAAGCAGG CGGGGCTGCC GTTGGAAAGC ACAGAGCACA AGCCACTGGT GGAGAGAGGC 
AGCAGGGTCT GGCCAGGAAT TGGAATTGTG GATCCTACAG ACCCTGAAGG GGACAGTGCG 
CGCCACATCA GCGCATCGGT CACCACGGCC AAGACCACAG TCGGCCACAT CTCAGCGGTG 
CAGGCTCGCG ACTCCACTCG CTCGCGGTTC CTGCAGGACA CAGAGGCGGT ACTGACCCGT 
ATCCGGGAGG AGAGCCGGCA GCAGCACTTT GATGCCCAGC GCTGGAGGGA TTGGTGGTGC 
CAGTCTGTAC GCAATATAAA AGCTCTGTAT ATGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747263.1
CDS	180..4838
Translation	MAAVGQIQAVPSGRVYRQLFDAEVQLVRSLGEARARAVQHGGAVDTGNIPPIRSRDNNTALPESHHQSTDKMTPPARKKQESEDDIAAREVRGLSDVIVPEKKDTGILERIAANRLERHEVALSNLHRELAEISKEMELSVLEVGKHLQQKISESDANIELLFQKLEIGNDSTTTKEINETWDAVTQESARTRQCIKRTESTLLALEENRAKRISEVLKKYTSLLKDICHLMPSDVDRFIHNEAMMINQAILANHRALARLSVNLLEANLKRENTQRFQWQDLKKAWECYQREIIMKEFRVFAEKEMEHIPTSVKVLVDQLTTQLSSLKERRLQILGLASFVPPTCTRTGVMEWYESLAALNSQAESINKQFLEKLQELREEFCQKFEVKAESCKEQLVSVDICTPEEAEDIITKELSPVTAQIHMKFKEEQEKWEKLLVHSSKLTILQTQKLLKFLEDVVDIWQTLERGLSKQENAVQLRMEQCRYKYDSDSKAKEANLDKILDRLRQDSTKETLRSTLGKALASLETIKAGYKTFHLDQVAVIDSYPALVLAELASYSCALSNYFGVKEVYGQVQKKDGENPSTSITTIPADGVRSHSREGISVNSEFNSYNYSAVESVLLEGEWLEGPGDGTHNTREDTEVPGIVQCDSTMAPVQTPYRESTQAAEGAGEQTSQGAQIPFELFTTSKGNTYTALRLASEDDTAVPEFPGEHSDTNIFMTEAVLDDSVPLNHDIMVIPEQQLIVIRKSVRLGFFEHLETWFGETLSSAQGIVSAKKEEFNSELALRYHLHEPRSQRIEMDIHNVRAAELILHSERVDRHCDGVSHSLEQLKRESSVLIENMKQQTLGFRSKIRAMESMFLGANKSDKLVALTQSLPSILDSHVSGVQTSMRNYRQHVEEMLGTLRDTNSDFIKSFRLFSEGGNFSPDEVEVFRKKLHKASDTIASFEGSIMVDLEGLESVCLEQATEIVKKFEDKFNMLTTDAIFLENIQKLLTNLQVKIKALVVSSNFQTQQINSSLEQLQQKTDACAHPNMDKETVTAEELYSFVQIPMGQLAKRSLYLSCLLEPSQILSETPLQGPIATASRSDTPLRQDAKGTFTTPDNLLTPSRVGKLALDDPAVSVIKNITRTKRVMDSQQDSDRASSYQRPGTVSHQAAAIAPHPPSQSGKPHLKKKKPPSSNGENPGQSVQAPSVRKLTKPSRFDKKYQLFGENRVESDNFKGILTCILWESNDTLLYLAEEFYKKKDRRNIGRTDLLQETFEECADTLVFKLQSYEKQALEYQNNCLLEFREQLQTFERLVNEVPPLVIESHRRQHLDKLLAATDQIRKQFAEDLQRWDQAKETLKSVLRPNLGHPDSEQALGELCQQEELRQREEAEGIDCNTKQLQACVSECTQHFLMSLASLTERLLLELDETLTVDDVLAADVEIPKEKLSTLIRRKQAGLPLESTEHKPLVERGSRVWPGIGIVDPTDPEGDSARHISASVTTAKTTVGHISAVQARDSTRSRFLQDTEAVLTRIREESRQQHFDAQRWRDWWCQSVRNIKALYMG

Target ORF information:

RefSeq Version	XM_031891403.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891403.1

ORF Insert Sequence:

ATGGCAGCAG TGGGACAGAT CCAGGCAGTT CCTAGTGGAA GAGTCTACAG GCAACTTTTT 
GATGCTGAGG TTCAGCTTGT GCGGTCCCTC GGAGAGGCTC GTGCTAGAGC GGTGCAGCAT 
GGAGGTGCAG TGGACACTGG GAACATTCCT CCAATCAGAA GCCGGGATAA CAACACAGCA 
TTGCCTGAGT CTCACCACCA GAGCACGGAT AAAATGACTC CACCTGCTAG GAAAAAGCAG 
GAAAGTGAGG ATGATATTGC AGCAAGAGAA GTGAGAGGTC TTTCTGATGT CATCGTTCCA 
GAAAAGAAAG ATACCGGCAT TCTTGAGCGG ATCGCTGCAA ACAGACTGGA ACGCCATGAA 
GTGGCACTGT CCAACCTGCA CAGGGAACTG GCTGAGATCA GCAAAGAGAT GGAGCTATCA 
GTATTGGAAG TGGGGAAGCA CCTGCAGCAA AAGATCTCGG AATCAGATGC AAATATTGAA 
CTTTTGTTTC AGAAACTGGA AATCGGTAAC GACAGCACCA CCACAAAGGA AATAAATGAG 
ACTTGGGATG CAGTGACCCA GGAATCTGCC CGTACAAGGC AGTGCATCAA AAGAACCGAA 
AGCACTTTAC TGGCGCTAGA AGAAAACAGA GCGAAACGAA TTTCTGAAGT CTTAAAGAAA 
TACACGTCCC TCTTAAAGGA CATCTGCCAT CTCATGCCCT CCGACGTGGA CAGATTTATT 
CATAATGAAG CCATGATGAT TAACCAGGCA ATTTTAGCTA ACCACCGAGC TCTGGCTAGA 
TTATCTGTAA ACCTTCTGGA AGCCAATCTG AAGAGGGAGA ATACCCAGCG CTTCCAATGG 
CAGGACCTGA AGAAGGCCTG GGAGTGTTAC CAGAGGGAGA TCATAATGAA GGAGTTCAGG 
GTGTTTGCTG AGAAAGAAAT GGAACATATA CCCACAAGTG TGAAAGTATT AGTGGATCAG 
CTGACCACCC AACTCAGCTC TCTCAAAGAA AGGAGACTTC AGATTCTGGG TTTAGCCAGT 
TTTGTACCTC CTACTTGCAC GAGAACAGGT GTCATGGAAT GGTATGAATC GTTGGCAGCC 
CTGAACAGTC AAGCTGAGTC CATCAACAAA CAATTCTTAG AGAAGCTTCA GGAGCTGCGG 
GAGGAATTCT GCCAAAAATT TGAGGTGAAA GCAGAAAGCT GTAAAGAGCA GTTGGTCAGT 
GTGGATATTT GTACCCCAGA GGAAGCTGAG GATATAATAA CCAAAGAACT CAGCCCAGTG 
ACGGCACAGA TCCACATGAA ATTTAAGGAA GAGCAAGAGA AGTGGGAAAA ACTGCTGGTA 
CATAGCTCTA AGTTAACGAT CCTTCAGACC CAGAAGTTGC TGAAATTCTT GGAAGATGTT 
GTTGATATTT GGCAAACGCT GGAACGTGGC CTCTCCAAAC AGGAAAATGC TGTGCAGCTA 
AGGATGGAGC AGTGTCGCTA TAAATATGAT AGTGACAGCA AGGCCAAGGA GGCAAATTTG 
GACAAGATAC TGGACAGACT GAGACAAGAC AGCACTAAGG AAACATTACG TAGCACATTG 
GGGAAAGCAC TGGCGTCCTT GGAAACCATA AAAGCAGGAT ACAAGACTTT TCATTTGGAC 
CAAGTCGCTG TAATTGATTC CTACCCTGCC CTGGTGCTTG CAGAGTTGGC CTCCTACAGC 
TGCGCTCTTA GCAATTATTT TGGGGTCAAA GAGGTGTATG GGCAGGTGCA GAAGAAGGAT 
GGGGAGAACC CCAGCACCAG TATAACAACG ATTCCGGCAG ATGGAGTGAG GTCTCATTCT 
AGAGAGGGGA TATCAGTAAA CTCCGAATTT AATTCCTACA ACTATTCTGC AGTAGAAAGT 
GTTTTGCTGG AAGGTGAATG GCTGGAAGGG CCAGGAGATG GAACCCACAA CACAAGAGAA 
GACACGGAAG TGCCTGGAAT AGTACAGTGT GATTCTACTA TGGCACCAGT GCAAACCCCT 
TATCGGGAGA GCACCCAAGC AGCAGAGGGA GCTGGTGAGC AGACATCACA GGGAGCCCAG 
ATTCCATTTG AGCTCTTCAC AACATCTAAA GGGAACACCT ATACTGCTTT GCGTTTGGCA 
TCAGAAGATG ACACGGCGGT GCCTGAGTTT CCAGGAGAAC ATTCCGACAC AAATATATTC 
ATGACGGAGG CGGTGCTGGA CGATTCCGTT CCCTTAAATC ATGACATAAT GGTCATACCC 
GAGCAACAGT TGATTGTTAT TAGGAAAAGC GTCCGGCTTG GGTTCTTTGA GCACCTGGAG 
ACCTGGTTCG GTGAGACCCT TTCCAGTGCC CAGGGTATCG TCAGTGCCAA GAAGGAAGAG 
TTCAATTCTG AGTTAGCGCT TCGCTATCAC CTGCACGAGC CAAGGAGCCA ACGGATCGAG 
ATGGATATCC ATAACGTCAG AGCTGCCGAG CTGATTCTGC ACTCCGAGAG AGTGGATCGT 
CACTGTGATG GGGTAAGCCA TTCTCTGGAG CAGCTGAAGC GGGAGAGTTC CGTGCTGATT 
GAGAACATGA AGCAACAGAC TCTCGGATTC CGCAGCAAGA TCCGGGCCAT GGAGAGCATG 
TTCCTCGGTG CCAACAAGTC TGATAAGCTT GTGGCCCTGA CACAGTCTCT GCCGTCCATT 
TTGGACAGCC ACGTAAGCGG GGTTCAGACT TCCATGCGCA ACTACCGGCA GCATGTAGAG 
GAAATGCTCG GAACCCTGCG CGACACAAAC TCGGACTTCA TCAAATCATT CAGATTGTTC 
TCAGAGGGCG GAAACTTTTC TCCCGATGAA GTGGAGGTGT TTCGCAAGAA GCTGCATAAA 
GCGTCAGACA CCATCGCCTC TTTCGAGGGC TCCATCATGG TGGATTTGGA GGGCTTGGAG 
TCTGTGTGTT TAGAACAGGC CACTGAGATT GTAAAGAAAT TTGAGGACAA ATTCAACATG 
TTAACAACGG ATGCAATTTT CCTAGAAAAC ATTCAGAAGC TGCTGACGAA CCTGCAAGTG 
AAAATCAAAG CCTTGGTTGT GAGCAGCAAT TTTCAGACTC AGCAGATCAA CTCCTCTCTG 
GAGCAGCTCC AACAGAAGAC AGATGCCTGC GCTCATCCCA ATATGGATAA GGAAACAGTC 
ACAGCTGAAG AACTGTACTC CTTTGTGCAA ATCCCCATGG GTCAGCTGGC AAAGAGAAGT 
CTCTATCTGT CCTGCCTTCT AGAGCCCAGC CAAATACTCT CTGAGACCCC ACTGCAAGGC 
CCTATTGCAA CGGCATCACG TTCGGACACC CCACTCCGTC AAGACGCGAA AGGAACCTTT 
ACGACCCCCG ACAACCTTCT TACTCCGAGC AGAGTGGGGA AGTTGGCTCT CGATGATCCC 
GCTGTGAGTG TCATCAAGAA CATTACCAGA ACAAAGCGGG TTATGGATTC ACAACAGGAC 
TCTGACCGTG CAAGTAGCTA TCAGCGGCCG GGCACAGTCT CACATCAGGC TGCTGCCATA 
GCGCCACATC CACCATCGCA GTCGGGTAAA CCTCATCTGA AGAAAAAGAA ACCCCCATCA 
AGCAATGGTG AAAATCCTGG CCAGTCTGTT CAGGCTCCAA GTGTCCGGAA GCTTACAAAG 
CCCAGCAGGT TTGACAAGAA GTACCAATTG TTTGGGGAGA ACAGAGTCGA ATCAGACAAC 
TTCAAGGGAA TTCTCACCTG TATACTGTGG GAGAGCAATG ACACCTTACT GTACCTGGCT 
GAGGAGTTCT ATAAGAAGAA GGACAGGCGG AACATTGGCC GGACAGATCT TCTTCAAGAG 
ACGTTTGAGG AGTGTGCAGA TACGCTGGTG TTTAAACTGC AGTCCTACGA GAAGCAGGCC 
CTGGAGTATC AGAACAACTG CCTGCTGGAG TTCCGGGAGC AGCTACAAAC TTTCGAGAGG 
CTGGTAAATG AGGTGCCGCC GCTGGTCATA GAGAGTCACC GGAGGCAACA CCTGGACAAA 
CTGCTGGCGG CCACTGACCA GATACGGAAG CAGTTTGCTG AGGACCTGCA GCGCTGGGAC 
CAAGCCAAGG AGACACTGAA GAGTGTGCTG AGGCCAAATT TAGGCCACCC AGACAGCGAG 
CAGGCCCTGG GGGAGCTGTG CCAGCAGGAG GAACTAAGGC AGAGAGAGGA GGCAGAAGGG 
ATTGACTGCA ACACTAAACA ATTACAAGCG TGCGTCTCCG AGTGCACCCA GCACTTCCTG 
ATGTCTCTGG CCTCCCTCAC CGAGAGACTG CTGCTGGAAC TCGATGAGAC ACTTACAGTC 
GATGATGTTT TAGCAGCTGA TGTTGAGATT CCCAAAGAAA AACTGTCAAC GCTGATCAGA 
CGCAAGCAGG CGGGGCTGCC GTTGGAAAGC ACAGAGCACA AGCCACTGGT GGAGAGAGGC 
AGCAGGGTCT GGCCAGGAAT TGGAATTGTG GATCCTACAG ACCCTGAAGG GGACAGTGCG 
CGCCACATCA GCGCATCGGT CACCACGGCC AAGACCACAG TCGGCCACAT CTCAGCGGTG 
CAGGCTCGCG ACTCCACTCG CTCGCGGTTC CTGCAGGACA CAGAGGCGGT ACTGACCCGT 
ATCCGGGAGG AGAGCCGGCA GCAGCACTTT GATGCCCAGC GCTGGAGGGA TTGGTGGTGC 
CAGTCTGTAC GCAATATAAA AGCTCTGTAT ATGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccdc180 ( XM_031891404.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747264.1 Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X4, mRNA.

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CloneID	OXa64703
Clone ID Related Accession (Same CDS sequence)	XM_031891404.1
Accession Version	XM_031891404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	coiled-coil domain-containing protein 180 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG TGGGACAGAT CCAGGCAGTT CCTAGTGGAA GAGTCTACAG GCAACTTTTT 
GATGCTGAGG TTCAGCTTGT GCGGTCCCTC GGAGAGGCTC GTGCTAGAGC GGTGCAGCAT 
GGAGGTGCAG TGGACACTGG GAACATTCCT CCAATCAGAA GCCGGGATAA CAACACAGCA 
TTGCCTGAGT CTCACCACCA GAGCACGGAT AAAATGACTC CACCTGCTAG GAAAAAGCAG 
GAAAGTGAGG ATGATATTGC AGCAAGAGAA GTGAGAGGTC TTTCTGATGT CATCGTTCCA 
GAAAAGAAAG ATACCGGCAT TCTTGAGCGG ATCGCTGCAA ACAGACTGGA ACGCCATGAA 
GTGGCACTGT CCAACCTGCA CAGGGAACTG GCTGAGATCA GCAAAGAGAT GGAGCTATCA 
GTATTGGAAG TGGGGAAGCA CCTGCAGCAA AAGATCTCGG AATCAGATGC AAATATTGAA 
CTTTTGTTTC AGAAACTGGA AATCGGTAAC GACAGCACCA CCACAAAGGA AATAAATGAG 
ACTTGGGATG CAGTGACCCA GGAATCTGCC CGTACAAGGC AGTGCATCAA AAGAACCGAA 
AGCACTTTAC TGGCGCTAGA AGAAAACAGA GCGAAACGAA TTTCTGAAGT CTTAAAGAAA 
TACACGTCCC TCTTAAAGGA CATCTGCCAT CTCATGCCCT CCGACGTGGA CAGATTTATT 
CATAATGAAG CCATGATGAT TAACCAGGCA ATTTTAGCTA ACCACCGAGC TCTGGCTAGA 
TTATCTGTAA ACCTTCTGGA AGCCAATCTG AAGAGGGAGA ATACCCAGCG CTTCCAATGG 
CAGGACCTGA AGAAGGCCTG GGAGTGTTAC CAGAGGGAGA TCATAATGAA GGAGTTCAGG 
GTGTTTGCTG AGAAAGAAAT GGAACATATA CCCACAAGTG TGAAAGTATT AGTGGATCAG 
CTGACCACCC AACTCAGCTC TCTCAAAGAA AGGAGACTTC AGATTCTGGG TTTAGCCAGA 
AGTTTTGTAC CTCCTACTTG CACGAGAACA GGTGTCATGG AATGGTATGA ATCGTTGGCA 
GCCCTGAACA GTCAAGCTGA GTCCATCAAC AAACAATTCT TAGAGAAGCT TCAGGAGCTG 
CGGGAGGAAT TCTGCCAAAA ATTTGAGGTG AAAGCAGAAA GCTGTAAATT GGTCAGTGTG 
GATATTTGTA CCCCAGAGGA AGCTGAGGAT ATAATAACCA AAGAACTCAG CCCAGTGACG 
GCACAGATCC ACATGAAATT TAAGGAAGAG CAAGAGAAGT GGGAAAAACT GCTGGTACAT 
AGCTCTAAGT TAACGATCCT TCAGACCCAG AAGTTGCTGA AATTCTTGGA AGATGTTGTT 
GATATTTGGC AAACGCTGGA ACGTGGCCTC TCCAAACAGG AAAATGCTGT GCAGCTAAGG 
ATGGAGCAGT GTCGCTATAA ATATGATAGT GACAGCAAGG CCAAGGAGGC AAATTTGGAC 
AAGATACTGG ACAGACTGAG ACAAGACAGC ACTAAGGAAA CATTACGTAG CACATTGGGG 
AAAGCACTGG CGTCCTTGGA AACCATAAAA GCAGGATACA AGACTTTTCA TTTGGACCAA 
GTCGCTGTAA TTGATTCCTA CCCTGCCCTG GTGCTTGCAG AGTTGGCCTC CTACAGCTGC 
GCTCTTAGCA ATTATTTTGG GGTCAAAGAG GTGTATGGGC AGGTGCAGAA GAAGGATGGG 
GAGAACCCCA GCACCAGTAT AACAACGATT CCGGCAGATG GAGTGAGGTC TCATTCTAGA 
GAGGGGATAT CAGTAAACTC CGAATTTAAT TCCTACAACT ATTCTGCAGT AGAAAGTGTT 
TTGCTGGAAG GTGAATGGCT GGAAGGGCCA GGAGATGGAA CCCACAACAC AAGAGAAGAC 
ACGGAAGTGC CTGGAATAGT ACAGTGTGAT TCTACTATGG CACCAGTGCA AACCCCTTAT 
CGGGAGAGCA CCCAAGCAGC AGAGGGAGCT GGTGAGCAGA CATCACAGGG AGCCCAGATT 
CCATTTGAGC TCTTCACAAC ATCTAAAGGG AACACCTATA CTGCTTTGCG TTTGGCATCA 
GAAGATGACA CGGCGGTGCC TGAGTTTCCA GGAGAACATT CCGACACAAA TATATTCATG 
ACGGAGGCGG TGCTGGACGA TTCCGTTCCC TTAAATCATG ACATAATGGT CATACCCGAG 
CAACAGTTGA TTGTTATTAG GAAAAGCGTC CGGCTTGGGT TCTTTGAGCA CCTGGAGACC 
TGGTTCGGTG AGACCCTTTC CAGTGCCCAG GGTATCGTCA GTGCCAAGAA GGAAGAGTTC 
AATTCTGAGT TAGCGCTTCG CTATCACCTG CACGAGCCAA GGAGCCAACG GATCGAGATG 
GATATCCATA ACGTCAGAGC TGCCGAGCTG ATTCTGCACT CCGAGAGAGT GGATCGTCAC 
TGTGATGGGG TAAGCCATTC TCTGGAGCAG CTGAAGCGGG AGAGTTCCGT GCTGATTGAG 
AACATGAAGC AACAGACTCT CGGATTCCGC AGCAAGATCC GGGCCATGGA GAGCATGTTC 
CTCGGTGCCA ACAAGTCTGA TAAGCTTGTG GCCCTGACAC AGTCTCTGCC GTCCATTTTG 
GACAGCCACG TAAGCGGGGT TCAGACTTCC ATGCGCAACT ACCGGCAGCA TGTAGAGGAA 
ATGCTCGGAA CCCTGCGCGA CACAAACTCG GACTTCATCA AATCATTCAG ATTGTTCTCA 
GAGGGCGGAA ACTTTTCTCC CGATGAAGTG GAGGTGTTTC GCAAGAAGCT GCATAAAGCG 
TCAGACACCA TCGCCTCTTT CGAGGGCTCC ATCATGGTGG ATTTGGAGGG CTTGGAGTCT 
GTGTGTTTAG AACAGGCCAC TGAGATTGTA AAGAAATTTG AGGACAAATT CAACATGTTA 
ACAACGGATG CAATTTTCCT AGAAAACATT CAGAAGCTGC TGACGAACCT GCAAGTGAAA 
ATCAAAGCCT TGGTTGTGAG CAGCAATTTT CAGACTCAGC AGATCAACTC CTCTCTGGAG 
CAGCTCCAAC AGAAGACAGA TGCCTGCGCT CATCCCAATA TGGATAAGGA AACAGTCACA 
GCTGAAGAAC TGTACTCCTT TGTGCAAATC CCCATGGGTC AGCTGGCAAA GAGAAGTCTC 
TATCTGTCCT GCCTTCTAGA GCCCAGCCAA ATACTCTCTG AGACCCCACT GCAAGGCCCT 
ATTGCAACGG CATCACGTTC GGACACCCCA CTCCGTCAAG ACGCGAAAGG AACCTTTACG 
ACCCCCGACA ACCTTCTTAC TCCGAGCAGA GTGGGGAAGT TGGCTCTCGA TGATCCCGCT 
GTGAGTGTCA TCAAGAACAT TACCAGAACA AAGCGGGTTA TGGATTCACA ACAGGACTCT 
GACCGTGCAA GTAGCTATCA GCGGCCGGGC ACAGTCTCAC ATCAGGCTGC TGCCATAGCG 
CCACATCCAC CATCGCAGTC GGGTAAACCT CATCTGAAGA AAAAGAAACC CCCATCAAGC 
AATGGTGAAA ATCCTGGCCA GTCTGTTCAG GCTCCAAGTG TCCGGAAGCT TACAAAGCCC 
AGCAGGTTTG ACAAGAAGTA CCAATTGTTT GGGGAGAACA GAGTCGAATC AGACAACTTC 
AAGGGAATTC TCACCTGTAT ACTGTGGGAG AGCAATGACA CCTTACTGTA CCTGGCTGAG 
GAGTTCTATA AGAAGAAGGA CAGGCGGAAC ATTGGCCGGA CAGATCTTCT TCAAGAGACG 
TTTGAGGAGT GTGCAGATAC GCTGGTGTTT AAACTGCAGT CCTACGAGAA GCAGGCCCTG 
GAGTATCAGA ACAACTGCCT GCTGGAGTTC CGGGAGCAGC TACAAACTTT CGAGAGGCTG 
GTAAATGAGG TGCCGCCGCT GGTCATAGAG AGTCACCGGA GGCAACACCT GGACAAACTG 
CTGGCGGCCA CTGACCAGAT ACGGAAGCAG TTTGCTGAGG ACCTGCAGCG CTGGGACCAA 
GCCAAGGAGA CACTGAAGAG TGTGCTGAGG CCAAATTTAG GCCACCCAGA CAGCGAGCAG 
GCCCTGGGGG AGCTGTGCCA GCAGGAGGAA CTAAGGCAGA GAGAGGAGGC AGAAGGGATT 
GACTGCAACA CTAAACAATT ACAAGCGTGC GTCTCCGAGT GCACCCAGCA CTTCCTGATG 
TCTCTGGCCT CCCTCACCGA GAGACTGCTG CTGGAACTCG ATGAGACACT TACAGTCGAT 
GATGTTTTAG CAGCTGATGT TGAGATTCCC AAAGAAAAAC TGTCAACGCT GATCAGACGC 
AAGCAGGCGG GGCTGCCGTT GGAAAGCACA GAGCACAAGC CACTGGTGGA GAGAGGCAGC 
AGGGTCTGGC CAGGAATTGG AATTGTGGAT CCTACAGACC CTGAAGGGGA CAGTGCGCGC 
CACATCAGCG CATCGGTCAC CACGGCCAAG ACCACAGTCG GCCACATCTC AGCGGTGCAG 
GCTCGCGACT CCACTCGCTC GCGGTTCCTG CAGGACACAG AGGCGGTACT GACCCGTATC 
CGGGAGGAGA GCCGGCAGCA GCACTTTGAT GCCCAGCGCT GGAGGGATTG GTGGTGCCAG 
TCTGTACGCA ATATAAAAGC TCTGTATATG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747264.1
CDS	180..4835
Translation	MAAVGQIQAVPSGRVYRQLFDAEVQLVRSLGEARARAVQHGGAVDTGNIPPIRSRDNNTALPESHHQSTDKMTPPARKKQESEDDIAAREVRGLSDVIVPEKKDTGILERIAANRLERHEVALSNLHRELAEISKEMELSVLEVGKHLQQKISESDANIELLFQKLEIGNDSTTTKEINETWDAVTQESARTRQCIKRTESTLLALEENRAKRISEVLKKYTSLLKDICHLMPSDVDRFIHNEAMMINQAILANHRALARLSVNLLEANLKRENTQRFQWQDLKKAWECYQREIIMKEFRVFAEKEMEHIPTSVKVLVDQLTTQLSSLKERRLQILGLARSFVPPTCTRTGVMEWYESLAALNSQAESINKQFLEKLQELREEFCQKFEVKAESCKLVSVDICTPEEAEDIITKELSPVTAQIHMKFKEEQEKWEKLLVHSSKLTILQTQKLLKFLEDVVDIWQTLERGLSKQENAVQLRMEQCRYKYDSDSKAKEANLDKILDRLRQDSTKETLRSTLGKALASLETIKAGYKTFHLDQVAVIDSYPALVLAELASYSCALSNYFGVKEVYGQVQKKDGENPSTSITTIPADGVRSHSREGISVNSEFNSYNYSAVESVLLEGEWLEGPGDGTHNTREDTEVPGIVQCDSTMAPVQTPYRESTQAAEGAGEQTSQGAQIPFELFTTSKGNTYTALRLASEDDTAVPEFPGEHSDTNIFMTEAVLDDSVPLNHDIMVIPEQQLIVIRKSVRLGFFEHLETWFGETLSSAQGIVSAKKEEFNSELALRYHLHEPRSQRIEMDIHNVRAAELILHSERVDRHCDGVSHSLEQLKRESSVLIENMKQQTLGFRSKIRAMESMFLGANKSDKLVALTQSLPSILDSHVSGVQTSMRNYRQHVEEMLGTLRDTNSDFIKSFRLFSEGGNFSPDEVEVFRKKLHKASDTIASFEGSIMVDLEGLESVCLEQATEIVKKFEDKFNMLTTDAIFLENIQKLLTNLQVKIKALVVSSNFQTQQINSSLEQLQQKTDACAHPNMDKETVTAEELYSFVQIPMGQLAKRSLYLSCLLEPSQILSETPLQGPIATASRSDTPLRQDAKGTFTTPDNLLTPSRVGKLALDDPAVSVIKNITRTKRVMDSQQDSDRASSYQRPGTVSHQAAAIAPHPPSQSGKPHLKKKKPPSSNGENPGQSVQAPSVRKLTKPSRFDKKYQLFGENRVESDNFKGILTCILWESNDTLLYLAEEFYKKKDRRNIGRTDLLQETFEECADTLVFKLQSYEKQALEYQNNCLLEFREQLQTFERLVNEVPPLVIESHRRQHLDKLLAATDQIRKQFAEDLQRWDQAKETLKSVLRPNLGHPDSEQALGELCQQEELRQREEAEGIDCNTKQLQACVSECTQHFLMSLASLTERLLLELDETLTVDDVLAADVEIPKEKLSTLIRRKQAGLPLESTEHKPLVERGSRVWPGIGIVDPTDPEGDSARHISASVTTAKTTVGHISAVQARDSTRSRFLQDTEAVLTRIREESRQQHFDAQRWRDWWCQSVRNIKALYMG

Target ORF information:

RefSeq Version	XM_031891404.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891404.1

ORF Insert Sequence:

ATGGCAGCAG TGGGACAGAT CCAGGCAGTT CCTAGTGGAA GAGTCTACAG GCAACTTTTT 
GATGCTGAGG TTCAGCTTGT GCGGTCCCTC GGAGAGGCTC GTGCTAGAGC GGTGCAGCAT 
GGAGGTGCAG TGGACACTGG GAACATTCCT CCAATCAGAA GCCGGGATAA CAACACAGCA 
TTGCCTGAGT CTCACCACCA GAGCACGGAT AAAATGACTC CACCTGCTAG GAAAAAGCAG 
GAAAGTGAGG ATGATATTGC AGCAAGAGAA GTGAGAGGTC TTTCTGATGT CATCGTTCCA 
GAAAAGAAAG ATACCGGCAT TCTTGAGCGG ATCGCTGCAA ACAGACTGGA ACGCCATGAA 
GTGGCACTGT CCAACCTGCA CAGGGAACTG GCTGAGATCA GCAAAGAGAT GGAGCTATCA 
GTATTGGAAG TGGGGAAGCA CCTGCAGCAA AAGATCTCGG AATCAGATGC AAATATTGAA 
CTTTTGTTTC AGAAACTGGA AATCGGTAAC GACAGCACCA CCACAAAGGA AATAAATGAG 
ACTTGGGATG CAGTGACCCA GGAATCTGCC CGTACAAGGC AGTGCATCAA AAGAACCGAA 
AGCACTTTAC TGGCGCTAGA AGAAAACAGA GCGAAACGAA TTTCTGAAGT CTTAAAGAAA 
TACACGTCCC TCTTAAAGGA CATCTGCCAT CTCATGCCCT CCGACGTGGA CAGATTTATT 
CATAATGAAG CCATGATGAT TAACCAGGCA ATTTTAGCTA ACCACCGAGC TCTGGCTAGA 
TTATCTGTAA ACCTTCTGGA AGCCAATCTG AAGAGGGAGA ATACCCAGCG CTTCCAATGG 
CAGGACCTGA AGAAGGCCTG GGAGTGTTAC CAGAGGGAGA TCATAATGAA GGAGTTCAGG 
GTGTTTGCTG AGAAAGAAAT GGAACATATA CCCACAAGTG TGAAAGTATT AGTGGATCAG 
CTGACCACCC AACTCAGCTC TCTCAAAGAA AGGAGACTTC AGATTCTGGG TTTAGCCAGA 
AGTTTTGTAC CTCCTACTTG CACGAGAACA GGTGTCATGG AATGGTATGA ATCGTTGGCA 
GCCCTGAACA GTCAAGCTGA GTCCATCAAC AAACAATTCT TAGAGAAGCT TCAGGAGCTG 
CGGGAGGAAT TCTGCCAAAA ATTTGAGGTG AAAGCAGAAA GCTGTAAATT GGTCAGTGTG 
GATATTTGTA CCCCAGAGGA AGCTGAGGAT ATAATAACCA AAGAACTCAG CCCAGTGACG 
GCACAGATCC ACATGAAATT TAAGGAAGAG CAAGAGAAGT GGGAAAAACT GCTGGTACAT 
AGCTCTAAGT TAACGATCCT TCAGACCCAG AAGTTGCTGA AATTCTTGGA AGATGTTGTT 
GATATTTGGC AAACGCTGGA ACGTGGCCTC TCCAAACAGG AAAATGCTGT GCAGCTAAGG 
ATGGAGCAGT GTCGCTATAA ATATGATAGT GACAGCAAGG CCAAGGAGGC AAATTTGGAC 
AAGATACTGG ACAGACTGAG ACAAGACAGC ACTAAGGAAA CATTACGTAG CACATTGGGG 
AAAGCACTGG CGTCCTTGGA AACCATAAAA GCAGGATACA AGACTTTTCA TTTGGACCAA 
GTCGCTGTAA TTGATTCCTA CCCTGCCCTG GTGCTTGCAG AGTTGGCCTC CTACAGCTGC 
GCTCTTAGCA ATTATTTTGG GGTCAAAGAG GTGTATGGGC AGGTGCAGAA GAAGGATGGG 
GAGAACCCCA GCACCAGTAT AACAACGATT CCGGCAGATG GAGTGAGGTC TCATTCTAGA 
GAGGGGATAT CAGTAAACTC CGAATTTAAT TCCTACAACT ATTCTGCAGT AGAAAGTGTT 
TTGCTGGAAG GTGAATGGCT GGAAGGGCCA GGAGATGGAA CCCACAACAC AAGAGAAGAC 
ACGGAAGTGC CTGGAATAGT ACAGTGTGAT TCTACTATGG CACCAGTGCA AACCCCTTAT 
CGGGAGAGCA CCCAAGCAGC AGAGGGAGCT GGTGAGCAGA CATCACAGGG AGCCCAGATT 
CCATTTGAGC TCTTCACAAC ATCTAAAGGG AACACCTATA CTGCTTTGCG TTTGGCATCA 
GAAGATGACA CGGCGGTGCC TGAGTTTCCA GGAGAACATT CCGACACAAA TATATTCATG 
ACGGAGGCGG TGCTGGACGA TTCCGTTCCC TTAAATCATG ACATAATGGT CATACCCGAG 
CAACAGTTGA TTGTTATTAG GAAAAGCGTC CGGCTTGGGT TCTTTGAGCA CCTGGAGACC 
TGGTTCGGTG AGACCCTTTC CAGTGCCCAG GGTATCGTCA GTGCCAAGAA GGAAGAGTTC 
AATTCTGAGT TAGCGCTTCG CTATCACCTG CACGAGCCAA GGAGCCAACG GATCGAGATG 
GATATCCATA ACGTCAGAGC TGCCGAGCTG ATTCTGCACT CCGAGAGAGT GGATCGTCAC 
TGTGATGGGG TAAGCCATTC TCTGGAGCAG CTGAAGCGGG AGAGTTCCGT GCTGATTGAG 
AACATGAAGC AACAGACTCT CGGATTCCGC AGCAAGATCC GGGCCATGGA GAGCATGTTC 
CTCGGTGCCA ACAAGTCTGA TAAGCTTGTG GCCCTGACAC AGTCTCTGCC GTCCATTTTG 
GACAGCCACG TAAGCGGGGT TCAGACTTCC ATGCGCAACT ACCGGCAGCA TGTAGAGGAA 
ATGCTCGGAA CCCTGCGCGA CACAAACTCG GACTTCATCA AATCATTCAG ATTGTTCTCA 
GAGGGCGGAA ACTTTTCTCC CGATGAAGTG GAGGTGTTTC GCAAGAAGCT GCATAAAGCG 
TCAGACACCA TCGCCTCTTT CGAGGGCTCC ATCATGGTGG ATTTGGAGGG CTTGGAGTCT 
GTGTGTTTAG AACAGGCCAC TGAGATTGTA AAGAAATTTG AGGACAAATT CAACATGTTA 
ACAACGGATG CAATTTTCCT AGAAAACATT CAGAAGCTGC TGACGAACCT GCAAGTGAAA 
ATCAAAGCCT TGGTTGTGAG CAGCAATTTT CAGACTCAGC AGATCAACTC CTCTCTGGAG 
CAGCTCCAAC AGAAGACAGA TGCCTGCGCT CATCCCAATA TGGATAAGGA AACAGTCACA 
GCTGAAGAAC TGTACTCCTT TGTGCAAATC CCCATGGGTC AGCTGGCAAA GAGAAGTCTC 
TATCTGTCCT GCCTTCTAGA GCCCAGCCAA ATACTCTCTG AGACCCCACT GCAAGGCCCT 
ATTGCAACGG CATCACGTTC GGACACCCCA CTCCGTCAAG ACGCGAAAGG AACCTTTACG 
ACCCCCGACA ACCTTCTTAC TCCGAGCAGA GTGGGGAAGT TGGCTCTCGA TGATCCCGCT 
GTGAGTGTCA TCAAGAACAT TACCAGAACA AAGCGGGTTA TGGATTCACA ACAGGACTCT 
GACCGTGCAA GTAGCTATCA GCGGCCGGGC ACAGTCTCAC ATCAGGCTGC TGCCATAGCG 
CCACATCCAC CATCGCAGTC GGGTAAACCT CATCTGAAGA AAAAGAAACC CCCATCAAGC 
AATGGTGAAA ATCCTGGCCA GTCTGTTCAG GCTCCAAGTG TCCGGAAGCT TACAAAGCCC 
AGCAGGTTTG ACAAGAAGTA CCAATTGTTT GGGGAGAACA GAGTCGAATC AGACAACTTC 
AAGGGAATTC TCACCTGTAT ACTGTGGGAG AGCAATGACA CCTTACTGTA CCTGGCTGAG 
GAGTTCTATA AGAAGAAGGA CAGGCGGAAC ATTGGCCGGA CAGATCTTCT TCAAGAGACG 
TTTGAGGAGT GTGCAGATAC GCTGGTGTTT AAACTGCAGT CCTACGAGAA GCAGGCCCTG 
GAGTATCAGA ACAACTGCCT GCTGGAGTTC CGGGAGCAGC TACAAACTTT CGAGAGGCTG 
GTAAATGAGG TGCCGCCGCT GGTCATAGAG AGTCACCGGA GGCAACACCT GGACAAACTG 
CTGGCGGCCA CTGACCAGAT ACGGAAGCAG TTTGCTGAGG ACCTGCAGCG CTGGGACCAA 
GCCAAGGAGA CACTGAAGAG TGTGCTGAGG CCAAATTTAG GCCACCCAGA CAGCGAGCAG 
GCCCTGGGGG AGCTGTGCCA GCAGGAGGAA CTAAGGCAGA GAGAGGAGGC AGAAGGGATT 
GACTGCAACA CTAAACAATT ACAAGCGTGC GTCTCCGAGT GCACCCAGCA CTTCCTGATG 
TCTCTGGCCT CCCTCACCGA GAGACTGCTG CTGGAACTCG ATGAGACACT TACAGTCGAT 
GATGTTTTAG CAGCTGATGT TGAGATTCCC AAAGAAAAAC TGTCAACGCT GATCAGACGC 
AAGCAGGCGG GGCTGCCGTT GGAAAGCACA GAGCACAAGC CACTGGTGGA GAGAGGCAGC 
AGGGTCTGGC CAGGAATTGG AATTGTGGAT CCTACAGACC CTGAAGGGGA CAGTGCGCGC 
CACATCAGCG CATCGGTCAC CACGGCCAAG ACCACAGTCG GCCACATCTC AGCGGTGCAG 
GCTCGCGACT CCACTCGCTC GCGGTTCCTG CAGGACACAG AGGCGGTACT GACCCGTATC 
CGGGAGGAGA GCCGGCAGCA GCACTTTGAT GCCCAGCGCT GGAGGGATTG GTGGTGCCAG 
TCTGTACGCA ATATAAAAGC TCTGTATATG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccdc180 ( XM_031891405.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747265.1 Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X5, mRNA.

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CloneID	OXa64704
Clone ID Related Accession (Same CDS sequence)	XM_031891405.1
Accession Version	XM_031891405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	coiled-coil domain-containing protein 180 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGT TCAGCTTGTG CGGTCCCTCG GAGAGGCTCG TGCTAGAGCG GTGCAGCATG 
GAGGTGCAGT GGACACTGGG AACATTCCTC CAATCAGAAG CCGGGATAAC AACACAGCAT 
TGCCTGAGTC TCACCACCAG AGCACGTAGG GATAAAATGA CTCCACCTGC TAGGAAAAAG 
CAGGAAAGTG AGGATGATAT TGCAGCAAGA GAAGTGAGAG GTCTTTCTGA TGTCATCGTT 
CCAGAAAAGA AAGATACCGG CATTCTTGAG CGGATCGCTG CAAACAGACT GGAACGCCAT 
GAAGTGGCAC TGTCCAACCT GCACAGGGAA CTGGCTGAGA TCAGCAAAGA GATGGAGCTA 
TCAGTATTGG AAGTGGGGAA GCACCTGCAG CAAAAGATCT CGGAATCAGA TGCAAATATT 
GAACTTTTGT TTCAGAAACT GGAAATCGGT AACGACAGCA CCACCACAAA GGAAATAAAT 
GAGACTTGGG ATGCAGTGAC CCAGGAATCT GCCCGTACAA GGCAGTGCAT CAAAAGAACC 
GAAAGCACTT TACTGGCGCT AGAAGAAAAC AGAGCGAAAC GAATTTCTGA AGTCTTAAAG 
AAATACACGT CCCTCTTAAA GGACATCTGC CATCTCATGC CCTCCGACGT GGACAGATTT 
ATTCATAATG AAGCCATGAT GATTAACCAG GCAATTTTAG CTAACCACCG AGCTCTGGCT 
AGATTATCTG TAAACCTTCT GGAAGCCAAT CTGAAGAGGG AGAATACCCA GCGCTTCCAA 
TGGCAGGACC TGAAGAAGGC CTGGGAGTGT TACCAGAGGG AGATCATAAT GAAGGAGTTC 
AGGGTGTTTG CTGAGAAAGA AATGGAACAT ATACCCACAA GTGTGAAAGT ATTAGTGGAT 
CAGCTGACCA CCCAACTCAG CTCTCTCAAA GAAAGGAGAC TTCAGATTCT GGGTTTAGCC 
AGAAGTTTTG TACCTCCTAC TTGCACGAGA ACAGGTGTCA TGGAATGGTA TGAATCGTTG 
GCAGCCCTGA ACAGTCAAGC TGAGTCCATC AACAAACAAT TCTTAGAGAA GCTTCAGGAG 
CTGCGGGAGG AATTCTGCCA AAAATTTGAG GTGAAAGCAG AAAGCTGTAA AGAGCAGTTG 
GTCAGTGTGG ATATTTGTAC CCCAGAGGAA GCTGAGGATA TAATAACCAA AGAACTCAGC 
CCAGTGACGG CACAGATCCA CATGAAATTT AAGGAAGAGC AAGAGAAGTG GGAAAAACTG 
CTGGTACATA GCTCTAAGTT AACGATCCTT CAGACCCAGA AGTTGCTGAA ATTCTTGGAA 
GATGTTGTTG ATATTTGGCA AACGCTGGAA CGTGGCCTCT CCAAACAGGA AAATGCTGTG 
CAGCTAAGGA TGGAGCAGTG TCGCTATAAA TATGATAGTG ACAGCAAGGC CAAGGAGGCA 
AATTTGGACA AGATACTGGA CAGACTGAGA CAAGACAGCA CTAAGGAAAC ATTACGTAGC 
ACATTGGGGA AAGCACTGGC GTCCTTGGAA ACCATAAAAG CAGGATACAA GACTTTTCAT 
TTGGACCAAG TCGCTGTAAT TGATTCCTAC CCTGCCCTGG TGCTTGCAGA GTTGGCCTCC 
TACAGCTGCG CTCTTAGCAA TTATTTTGGG GTCAAAGAGG TGTATGGGCA GGTGCAGAAG 
AAGGATGGGG AGAACCCCAG CACCAGTATA ACAACGATTC CGGCAGATGG AGTGAGGTCT 
CATTCTAGAG AGGGGATATC AGTAAACTCC GAATTTAATT CCTACAACTA TTCTGCAGTA 
GAAAGTGTTT TGCTGGAAGG TGAATGGCTG GAAGGGCCAG GAGATGGAAC CCACAACACA 
AGAGAAGACA CGGAAGTGCC TGGAATAGTA CAGTGTGATT CTACTATGGC ACCAGTGCAA 
ACCCCTTATC GGGAGAGCAC CCAAGCAGCA GAGGGAGCTG GTGAGCAGAC ATCACAGGGA 
GCCCAGATTC CATTTGAGCT CTTCACAACA TCTAAAGGGA ACACCTATAC TGCTTTGCGT 
TTGGCATCAG AAGATGACAC GGCGGTGCCT GAGTTTCCAG GAGAACATTC CGACACAAAT 
ATATTCATGA CGGAGGCGGT GCTGGACGAT TCCGTTCCCT TAAATCATGA CATAATGGTC 
ATACCCGAGC AACAGTTGAT TGTTATTAGG AAAAGCGTCC GGCTTGGGTT CTTTGAGCAC 
CTGGAGACCT GGTTCGGTGA GACCCTTTCC AGTGCCCAGG GTATCGTCAG TGCCAAGAAG 
GAAGAGTTCA ATTCTGAGTT AGCGCTTCGC TATCACCTGC ACGAGCCAAG GAGCCAACGG 
ATCGAGATGG ATATCCATAA CGTCAGAGCT GCCGAGCTGA TTCTGCACTC CGAGAGAGTG 
GATCGTCACT GTGATGGGGT AAGCCATTCT CTGGAGCAGC TGAAGCGGGA GAGTTCCGTG 
CTGATTGAGA ACATGAAGCA ACAGACTCTC GGATTCCGCA GCAAGATCCG GGCCATGGAG 
AGCATGTTCC TCGGTGCCAA CAAGTCTGAT AAGCTTGTGG CCCTGACACA GTCTCTGCCG 
TCCATTTTGG ACAGCCACGT AAGCGGGGTT CAGACTTCCA TGCGCAACTA CCGGCAGCAT 
GTAGAGGAAA TGCTCGGAAC CCTGCGCGAC ACAAACTCGG ACTTCATCAA ATCATTCAGA 
TTGTTCTCAG AGGGCGGAAA CTTTTCTCCC GATGAAGTGG AGGTGTTTCG CAAGAAGCTG 
CATAAAGCGT CAGACACCAT CGCCTCTTTC GAGGGCTCCA TCATGGTGGA TTTGGAGGGC 
TTGGAGTCTG TGTGTTTAGA ACAGGCCACT GAGATTGTAA AGAAATTTGA GGACAAATTC 
AACATGTTAA CAACGGATGC AATTTTCCTA GAAAACATTC AGAAGCTGCT GACGAACCTG 
CAAGTGAAAA TCAAAGCCTT GGTTGTGAGC AGCAATTTTC AGACTCAGCA GATCAACTCC 
TCTCTGGAGC AGCTCCAACA GAAGACAGAT GCCTGCGCTC ATCCCAATAT GGATAAGGAA 
ACAGTCACAG CTGAAGAACT GTACTCCTTT GTGCAAATCC CCATGGGTCA GCTGGCAAAG 
AGAAGTCTCT ATCTGTCCTG CCTTCTAGAG CCCAGCCAAA TACTCTCTGA GACCCCACTG 
CAAGGCCCTA TTGCAACGGC ATCACGTTCG GACACCCCAC TCCGTCAAGA CGCGAAAGGA 
ACCTTTACGA CCCCCGACAA CCTTCTTACT CCGAGCAGAG TGGGGAAGTT GGCTCTCGAT 
GATCCCGCTG TGAGTGTCAT CAAGAACATT ACCAGAACAA AGCGGGTTAT GGATTCACAA 
CAGGACTCTG ACCGTGCAAG TAGCTATCAG CGGCCGGGCA CAGTCTCACA TCAGGCTGCT 
GCCATAGCGC CACATCCACC ATCGCAGTCG GGTAAACCTC ATCTGAAGAA AAAGAAACCC 
CCATCAAGCA ATGGTGAAAA TCCTGGCCAG TCTGTTCAGG CTCCAAGTGT CCGGAAGCTT 
ACAAAGCCCA GCAGGTTTGA CAAGAAGTAC CAATTGTTTG GGGAGAACAG AGTCGAATCA 
GACAACTTCA AGGGAATTCT CACCTGTATA CTGTGGGAGA GCAATGACAC CTTACTGTAC 
CTGGCTGAGG AGTTCTATAA GAAGAAGGAC AGGCGGAACA TTGGCCGGAC AGATCTTCTT 
CAAGAGACGT TTGAGGAGTG TGCAGATACG CTGGTGTTTA AACTGCAGTC CTACGAGAAG 
CAGGCCCTGG AGTATCAGAA CAACTGCCTG CTGGAGTTCC GGGAGCAGCT ACAAACTTTC 
GAGAGGCTGG TAAATGAGGT GCCGCCGCTG GTCATAGAGA GTCACCGGAG GCAACACCTG 
GACAAACTGC TGGCGGCCAC TGACCAGATA CGGAAGCAGT TTGCTGAGGA CCTGCAGCGC 
TGGGACCAAG CCAAGGAGAC ACTGAAGAGT GTGCTGAGGC CAAATTTAGG CCACCCAGAC 
AGCGAGCAGG CCCTGGGGGA GCTGTGCCAG CAGGAGGAAC TAAGGCAGAG AGAGGAGGCA 
GAAGGGATTG ACTGCAACAC TAAACAATTA CAAGCGTGCG TCTCCGAGTG CACCCAGCAC 
TTCCTGATGT CTCTGGCCTC CCTCACCGAG AGACTGCTGC TGGAACTCGA TGAGACACTT 
ACAGTCGATG ATGTTTTAGC AGCTGATGTT GAGATTCCCA AAGAAAAACT GTCAACGCTG 
ATCAGACGCA AGCAGGCGGG GCTGCCGTTG GAAAGCACAG AGCACAAGCC ACTGGTGGAG 
AGAGGCAGCA GGGTCTGGCC AGGAATTGGA ATTGTGGATC CTACAGACCC TGAAGGGGAC 
AGTGCGCGCC ACATCAGCGC ATCGGTCACC ACGGCCAAGA CCACAGTCGG CCACATCTCA 
GCGGTGCAGG CTCGCGACTC CACTCGCTCG CGGTTCCTGC AGGACACAGA GGCGGTACTG 
ACCCGTATCC GGGAGGAGAG CCGGCAGCAG CACTTTGATG CCCAGCGCTG GAGGGATTGG 
TGGTGCCAGT CTGTACGCAA TATAAAAGCT CTGTATATGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747265.1
CDS	241..4845
Translation	MLRFSLCGPSERLVLERCSMEVQWTLGTFLQSEAGITTQHCLSLTTRARRDKMTPPARKKQESEDDIAAREVRGLSDVIVPEKKDTGILERIAANRLERHEVALSNLHRELAEISKEMELSVLEVGKHLQQKISESDANIELLFQKLEIGNDSTTTKEINETWDAVTQESARTRQCIKRTESTLLALEENRAKRISEVLKKYTSLLKDICHLMPSDVDRFIHNEAMMINQAILANHRALARLSVNLLEANLKRENTQRFQWQDLKKAWECYQREIIMKEFRVFAEKEMEHIPTSVKVLVDQLTTQLSSLKERRLQILGLARSFVPPTCTRTGVMEWYESLAALNSQAESINKQFLEKLQELREEFCQKFEVKAESCKEQLVSVDICTPEEAEDIITKELSPVTAQIHMKFKEEQEKWEKLLVHSSKLTILQTQKLLKFLEDVVDIWQTLERGLSKQENAVQLRMEQCRYKYDSDSKAKEANLDKILDRLRQDSTKETLRSTLGKALASLETIKAGYKTFHLDQVAVIDSYPALVLAELASYSCALSNYFGVKEVYGQVQKKDGENPSTSITTIPADGVRSHSREGISVNSEFNSYNYSAVESVLLEGEWLEGPGDGTHNTREDTEVPGIVQCDSTMAPVQTPYRESTQAAEGAGEQTSQGAQIPFELFTTSKGNTYTALRLASEDDTAVPEFPGEHSDTNIFMTEAVLDDSVPLNHDIMVIPEQQLIVIRKSVRLGFFEHLETWFGETLSSAQGIVSAKKEEFNSELALRYHLHEPRSQRIEMDIHNVRAAELILHSERVDRHCDGVSHSLEQLKRESSVLIENMKQQTLGFRSKIRAMESMFLGANKSDKLVALTQSLPSILDSHVSGVQTSMRNYRQHVEEMLGTLRDTNSDFIKSFRLFSEGGNFSPDEVEVFRKKLHKASDTIASFEGSIMVDLEGLESVCLEQATEIVKKFEDKFNMLTTDAIFLENIQKLLTNLQVKIKALVVSSNFQTQQINSSLEQLQQKTDACAHPNMDKETVTAEELYSFVQIPMGQLAKRSLYLSCLLEPSQILSETPLQGPIATASRSDTPLRQDAKGTFTTPDNLLTPSRVGKLALDDPAVSVIKNITRTKRVMDSQQDSDRASSYQRPGTVSHQAAAIAPHPPSQSGKPHLKKKKPPSSNGENPGQSVQAPSVRKLTKPSRFDKKYQLFGENRVESDNFKGILTCILWESNDTLLYLAEEFYKKKDRRNIGRTDLLQETFEECADTLVFKLQSYEKQALEYQNNCLLEFREQLQTFERLVNEVPPLVIESHRRQHLDKLLAATDQIRKQFAEDLQRWDQAKETLKSVLRPNLGHPDSEQALGELCQQEELRQREEAEGIDCNTKQLQACVSECTQHFLMSLASLTERLLLELDETLTVDDVLAADVEIPKEKLSTLIRRKQAGLPLESTEHKPLVERGSRVWPGIGIVDPTDPEGDSARHISASVTTAKTTVGHISAVQARDSTRSRFLQDTEAVLTRIREESRQQHFDAQRWRDWWCQSVRNIKALYMG

Target ORF information:

RefSeq Version	XM_031891405.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891405.1

ORF Insert Sequence:

ATGCTGAGGT TCAGCTTGTG CGGTCCCTCG GAGAGGCTCG TGCTAGAGCG GTGCAGCATG 
GAGGTGCAGT GGACACTGGG AACATTCCTC CAATCAGAAG CCGGGATAAC AACACAGCAT 
TGCCTGAGTC TCACCACCAG AGCACGTAGG GATAAAATGA CTCCACCTGC TAGGAAAAAG 
CAGGAAAGTG AGGATGATAT TGCAGCAAGA GAAGTGAGAG GTCTTTCTGA TGTCATCGTT 
CCAGAAAAGA AAGATACCGG CATTCTTGAG CGGATCGCTG CAAACAGACT GGAACGCCAT 
GAAGTGGCAC TGTCCAACCT GCACAGGGAA CTGGCTGAGA TCAGCAAAGA GATGGAGCTA 
TCAGTATTGG AAGTGGGGAA GCACCTGCAG CAAAAGATCT CGGAATCAGA TGCAAATATT 
GAACTTTTGT TTCAGAAACT GGAAATCGGT AACGACAGCA CCACCACAAA GGAAATAAAT 
GAGACTTGGG ATGCAGTGAC CCAGGAATCT GCCCGTACAA GGCAGTGCAT CAAAAGAACC 
GAAAGCACTT TACTGGCGCT AGAAGAAAAC AGAGCGAAAC GAATTTCTGA AGTCTTAAAG 
AAATACACGT CCCTCTTAAA GGACATCTGC CATCTCATGC CCTCCGACGT GGACAGATTT 
ATTCATAATG AAGCCATGAT GATTAACCAG GCAATTTTAG CTAACCACCG AGCTCTGGCT 
AGATTATCTG TAAACCTTCT GGAAGCCAAT CTGAAGAGGG AGAATACCCA GCGCTTCCAA 
TGGCAGGACC TGAAGAAGGC CTGGGAGTGT TACCAGAGGG AGATCATAAT GAAGGAGTTC 
AGGGTGTTTG CTGAGAAAGA AATGGAACAT ATACCCACAA GTGTGAAAGT ATTAGTGGAT 
CAGCTGACCA CCCAACTCAG CTCTCTCAAA GAAAGGAGAC TTCAGATTCT GGGTTTAGCC 
AGAAGTTTTG TACCTCCTAC TTGCACGAGA ACAGGTGTCA TGGAATGGTA TGAATCGTTG 
GCAGCCCTGA ACAGTCAAGC TGAGTCCATC AACAAACAAT TCTTAGAGAA GCTTCAGGAG 
CTGCGGGAGG AATTCTGCCA AAAATTTGAG GTGAAAGCAG AAAGCTGTAA AGAGCAGTTG 
GTCAGTGTGG ATATTTGTAC CCCAGAGGAA GCTGAGGATA TAATAACCAA AGAACTCAGC 
CCAGTGACGG CACAGATCCA CATGAAATTT AAGGAAGAGC AAGAGAAGTG GGAAAAACTG 
CTGGTACATA GCTCTAAGTT AACGATCCTT CAGACCCAGA AGTTGCTGAA ATTCTTGGAA 
GATGTTGTTG ATATTTGGCA AACGCTGGAA CGTGGCCTCT CCAAACAGGA AAATGCTGTG 
CAGCTAAGGA TGGAGCAGTG TCGCTATAAA TATGATAGTG ACAGCAAGGC CAAGGAGGCA 
AATTTGGACA AGATACTGGA CAGACTGAGA CAAGACAGCA CTAAGGAAAC ATTACGTAGC 
ACATTGGGGA AAGCACTGGC GTCCTTGGAA ACCATAAAAG CAGGATACAA GACTTTTCAT 
TTGGACCAAG TCGCTGTAAT TGATTCCTAC CCTGCCCTGG TGCTTGCAGA GTTGGCCTCC 
TACAGCTGCG CTCTTAGCAA TTATTTTGGG GTCAAAGAGG TGTATGGGCA GGTGCAGAAG 
AAGGATGGGG AGAACCCCAG CACCAGTATA ACAACGATTC CGGCAGATGG AGTGAGGTCT 
CATTCTAGAG AGGGGATATC AGTAAACTCC GAATTTAATT CCTACAACTA TTCTGCAGTA 
GAAAGTGTTT TGCTGGAAGG TGAATGGCTG GAAGGGCCAG GAGATGGAAC CCACAACACA 
AGAGAAGACA CGGAAGTGCC TGGAATAGTA CAGTGTGATT CTACTATGGC ACCAGTGCAA 
ACCCCTTATC GGGAGAGCAC CCAAGCAGCA GAGGGAGCTG GTGAGCAGAC ATCACAGGGA 
GCCCAGATTC CATTTGAGCT CTTCACAACA TCTAAAGGGA ACACCTATAC TGCTTTGCGT 
TTGGCATCAG AAGATGACAC GGCGGTGCCT GAGTTTCCAG GAGAACATTC CGACACAAAT 
ATATTCATGA CGGAGGCGGT GCTGGACGAT TCCGTTCCCT TAAATCATGA CATAATGGTC 
ATACCCGAGC AACAGTTGAT TGTTATTAGG AAAAGCGTCC GGCTTGGGTT CTTTGAGCAC 
CTGGAGACCT GGTTCGGTGA GACCCTTTCC AGTGCCCAGG GTATCGTCAG TGCCAAGAAG 
GAAGAGTTCA ATTCTGAGTT AGCGCTTCGC TATCACCTGC ACGAGCCAAG GAGCCAACGG 
ATCGAGATGG ATATCCATAA CGTCAGAGCT GCCGAGCTGA TTCTGCACTC CGAGAGAGTG 
GATCGTCACT GTGATGGGGT AAGCCATTCT CTGGAGCAGC TGAAGCGGGA GAGTTCCGTG 
CTGATTGAGA ACATGAAGCA ACAGACTCTC GGATTCCGCA GCAAGATCCG GGCCATGGAG 
AGCATGTTCC TCGGTGCCAA CAAGTCTGAT AAGCTTGTGG CCCTGACACA GTCTCTGCCG 
TCCATTTTGG ACAGCCACGT AAGCGGGGTT CAGACTTCCA TGCGCAACTA CCGGCAGCAT 
GTAGAGGAAA TGCTCGGAAC CCTGCGCGAC ACAAACTCGG ACTTCATCAA ATCATTCAGA 
TTGTTCTCAG AGGGCGGAAA CTTTTCTCCC GATGAAGTGG AGGTGTTTCG CAAGAAGCTG 
CATAAAGCGT CAGACACCAT CGCCTCTTTC GAGGGCTCCA TCATGGTGGA TTTGGAGGGC 
TTGGAGTCTG TGTGTTTAGA ACAGGCCACT GAGATTGTAA AGAAATTTGA GGACAAATTC 
AACATGTTAA CAACGGATGC AATTTTCCTA GAAAACATTC AGAAGCTGCT GACGAACCTG 
CAAGTGAAAA TCAAAGCCTT GGTTGTGAGC AGCAATTTTC AGACTCAGCA GATCAACTCC 
TCTCTGGAGC AGCTCCAACA GAAGACAGAT GCCTGCGCTC ATCCCAATAT GGATAAGGAA 
ACAGTCACAG CTGAAGAACT GTACTCCTTT GTGCAAATCC CCATGGGTCA GCTGGCAAAG 
AGAAGTCTCT ATCTGTCCTG CCTTCTAGAG CCCAGCCAAA TACTCTCTGA GACCCCACTG 
CAAGGCCCTA TTGCAACGGC ATCACGTTCG GACACCCCAC TCCGTCAAGA CGCGAAAGGA 
ACCTTTACGA CCCCCGACAA CCTTCTTACT CCGAGCAGAG TGGGGAAGTT GGCTCTCGAT 
GATCCCGCTG TGAGTGTCAT CAAGAACATT ACCAGAACAA AGCGGGTTAT GGATTCACAA 
CAGGACTCTG ACCGTGCAAG TAGCTATCAG CGGCCGGGCA CAGTCTCACA TCAGGCTGCT 
GCCATAGCGC CACATCCACC ATCGCAGTCG GGTAAACCTC ATCTGAAGAA AAAGAAACCC 
CCATCAAGCA ATGGTGAAAA TCCTGGCCAG TCTGTTCAGG CTCCAAGTGT CCGGAAGCTT 
ACAAAGCCCA GCAGGTTTGA CAAGAAGTAC CAATTGTTTG GGGAGAACAG AGTCGAATCA 
GACAACTTCA AGGGAATTCT CACCTGTATA CTGTGGGAGA GCAATGACAC CTTACTGTAC 
CTGGCTGAGG AGTTCTATAA GAAGAAGGAC AGGCGGAACA TTGGCCGGAC AGATCTTCTT 
CAAGAGACGT TTGAGGAGTG TGCAGATACG CTGGTGTTTA AACTGCAGTC CTACGAGAAG 
CAGGCCCTGG AGTATCAGAA CAACTGCCTG CTGGAGTTCC GGGAGCAGCT ACAAACTTTC 
GAGAGGCTGG TAAATGAGGT GCCGCCGCTG GTCATAGAGA GTCACCGGAG GCAACACCTG 
GACAAACTGC TGGCGGCCAC TGACCAGATA CGGAAGCAGT TTGCTGAGGA CCTGCAGCGC 
TGGGACCAAG CCAAGGAGAC ACTGAAGAGT GTGCTGAGGC CAAATTTAGG CCACCCAGAC 
AGCGAGCAGG CCCTGGGGGA GCTGTGCCAG CAGGAGGAAC TAAGGCAGAG AGAGGAGGCA 
GAAGGGATTG ACTGCAACAC TAAACAATTA CAAGCGTGCG TCTCCGAGTG CACCCAGCAC 
TTCCTGATGT CTCTGGCCTC CCTCACCGAG AGACTGCTGC TGGAACTCGA TGAGACACTT 
ACAGTCGATG ATGTTTTAGC AGCTGATGTT GAGATTCCCA AAGAAAAACT GTCAACGCTG 
ATCAGACGCA AGCAGGCGGG GCTGCCGTTG GAAAGCACAG AGCACAAGCC ACTGGTGGAG 
AGAGGCAGCA GGGTCTGGCC AGGAATTGGA ATTGTGGATC CTACAGACCC TGAAGGGGAC 
AGTGCGCGCC ACATCAGCGC ATCGGTCACC ACGGCCAAGA CCACAGTCGG CCACATCTCA 
GCGGTGCAGG CTCGCGACTC CACTCGCTCG CGGTTCCTGC AGGACACAGA GGCGGTACTG 
ACCCGTATCC GGGAGGAGAG CCGGCAGCAG CACTTTGATG CCCAGCGCTG GAGGGATTGG 
TGGTGCCAGT CTGTACGCAA TATAAAAGCT CTGTATATGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccdc180 ( XM_012968714.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824168.1 Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), mRNA.

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CloneID	OXa35406
Clone ID Related Accession (Same CDS sequence)	XM_012968714.2
Accession Version	XM_012968714.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	coiled-coil domain-containing protein 180
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683403.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968714.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGCAG TGGGACAGAT CCAGGCAGTT CCTAGTGGAA GAGTCTACAG GCAACTTTTT 
GATGCTGAGG TTCAGCTTGT GCGGTCCCTC GGAGAGGCTC GTGCTAGAGC GGTGCAGCAT 
GGAGGTGCAG TGGACACTGG GAACATTCCT CCAATCAGAA GCCGGGATAA CAACACAGCA 
TTGCCTGAGT CTCACCACCA GAGCACGGAT AAAATGACTC CACCTGCTAG GAAAAAGCAG 
GAAAGTGAGG ATGATATTGC AGCAAGAGAA GTGAGAGGTC TTTCTGATGT CATCGTTCCA 
GAAAAGAAAG ATACCGGCAT TCTTGAGCGG ATCGCTGCAA ACAGACTGGA ACGCCATGAA 
GTGGCACTGT CCAACCTGCA CAGGGAACTG GCTGAGATCA GCAAAGAGAT GGAGCTATCA 
GTATTGGAAG TGGGGAAGCA CCTGCAGCAA AAGATCTCGG AATCAGATGC AAATATTGAA 
CTTTTGTTTC AGAAACTGGA AATCGGTAAC GACAGCACCA CCACAAAGGA AATAAATGAG 
ACTTGGGATG CAGTGACCCA GGAATCTGCC CGTACAAGGC AGTGCATCAA AAGAACCGAA 
AGCACTTTAC TGGCGCTAGA AGAAAACAGA GCGAAACGAA TTTCTGAAGT CTTAAAGAAA 
TACACGTCCC TCTTAAAGGA CATCTGCCAT CTCATGCCCT CCGACGTGGA CAGATTTATT 
CATAATGAAG CCATGATGAT TAACCAGGCA ATTTTAGCTA ACCACCGAGC TCTGGCTAGA 
TTATCTGTAA ACCTTCTGGA AGCCAATCTG AAGAGGGAGA ATACCCAGCG CTTCCAATGG 
CAGGACCTGA AGAAGGCCTG GGAGTGTTAC CAGAGGGAGA TCATAATGAA GGAGTTCAGG 
GTGTTTGCTG AGAAAGAAAT GGAACATATA CCCACAAGTG TGAAAGTATT AGTGGATCAG 
CTGACCACCC AACTCAGCTC TCTCAAAGAA AGGAGACTTC AGATTCTGGG TTTAGCCAGA 
AGTTTTGTAC CTCCTACTTG CACGAGAACA GGTGTCATGG AATGGTATGA ATCGTTGGCA 
GCCCTGAACA GTCAAGCTGA GTCCATCAAC AAACAATTCT TAGAGAAGCT TCAGGAGCTG 
CGGGAGGAAT TCTGCCAAAA ATTTGAGGTG AAAGCAGAAA GCTGTAAAGA GCAGTTGGTC 
AGTGTGGATA TTTGTACCCC AGAGGAAGCT GAGGATATAA TAACCAAAGA ACTCAGCCCA 
GTGACGGCAC AGATCCACAT GAAATTTAAG GAAGAGCAAG AGAAGTGGGA AAAACTGCTG 
GTACATAGCT CTAAGTTAAC GATCCTTCAG ACCCAGAAGT TGCTGAAATT CTTGGAAGAT 
GTTGTTGATA TTTGGCAAAC GCTGGAACGT GGCCTCTCCA AACAGGAAAA TGCTGTGCAG 
CTAAGGATGG AGCAGTGTCG CTATAAATAT GATAGTGACA GCAAGGCCAA GGAGGCAAAT 
TTGGACAAGA TACTGGACAG ACTGAGACAA GACAGCACTA AGGAAACATT ACGTAGCACA 
TTGGGGAAAG CACTGGCGTC CTTGGAAACC ATAAAAGCAG GATACAAGAC TTTTCATTTG 
GACCAAGTCG CTGTAATTGA TTCCTACCCT GCCCTGGTGC TTGCAGAGTT GGCCTCCTAC 
AGCTGCGCTC TTAGCAATTA TTTTGGGGTC AAAGAGGTGT ATGGGCAGGT GCAGAAGAAG 
GATGGGGAGA ACCCCAGCAC CAGTATAACA ACGATTCCGG CAGATGGAGT GAGGTCTCAT 
TCTAGAGAGG GGATATCAGT AAACTCCGAA TTTAATTCCT ACAACTATTC TGCAGTAGAA 
AGTGTTTTGC TGGAAGGTGA ATGGCTGGAA GGGCCAGGAG ATGGAACCCA CAACACAAGA 
GAAGACACGG AAGTGCCTGG AATAGTACAG TGTGATTCTA CTATGGCACC AGTGCAAACC 
CCTTATCGGG AGAGCACCCA AGCAGCAGAG GGAGCTGGTG AGCAGACATC ACAGGGAGCC 
CAGATTCCAT TTGAGCTCTT CACAACATCT AAAGGGAACA CCTATACTGC TTTGCGTTTG 
GCATCAGAAG ATGACACGGC GGTGCCTGAG TTTCCAGGAG AACATTCCGA CACAAATATA 
TTCATGACGG AGGCGGTGCT GGACGATTCC GTTCCCTTAA ATCATGACAT AATGGTCATA 
CCCGAGCAAC AGTTGATTGT TATTAGGAAA AGCGTCCGGC TTGGGTTCTT TGAGCACCTG 
GAGACCTGGT TCGGTGAGAC CCTTTCCAGT GCCCAGGGTA TCGTCAGTGC CAAGAAGGAA 
GAGTTCAATT CTGAGTTAGC GCTTCGCTAT CACCTGCACG AGCCAAGGAG CCAACGGATC 
GAGATGGATA TCCATAACGT CAGAGCTGCC GAGCTGATTC TGCACTCCGA GAGAGTGGAT 
CGTCACTGTG ATGGGGTAAG CCATTCTCTG GAGCAGCTGA AGCGGGAGAG TTCCGTGCTG 
ATTGAGAACA TGAAGCAACA GACTCTCGGA TTCCGCAGCA AGATCCGGGC CATGGAGAGC 
ATGTTCCTCG GTGCCAACAA GTCTGATAAG CTTGTGGCCC TGACACAGTC TCTGCCGTCC 
ATTTTGGACA GCCACGTAAG CGGGGTTCAG ACTTCCATGC GCAACTACCG GCAGCATGTA 
GAGGAAATGC TCGGAACCCT GCGCGACACA AACTCGGACT TCATCAAATC ATTCAGATTG 
TTCTCAGAGG GCGGAAACTT TTCTCCCGAT GAAGTGGAGG TGTTTCGCAA GAAGCTGCAT 
AAAGCGTCAG ACACCATCGC CTCTTTCGAG GGCTCCATCA TGGTGGATTT GGAGGGCTTG 
GAGTCTGTGT GTTTAGAACA GGCCACTGAG ATTGTAAAGA AATTTGAGGA CAAATTCAAC 
ATGTTAACAA CGGATGCAAT TTTCCTAGAA AACATTCAGA AGCTGCTGAC GAACCTGCAA 
GTGAAAATCA AAGCCTTGGT TGTGAGCAGC AATTTTCAGA CTCAGCAGAT CAACTCCTCT 
CTGGAGCAGC TCCAACAGAA GACAGATGCC TGCGCTCATC CCAATATGGA TAAGGAAACA 
GTCACAGCTG AAGAACTGTA CTCCTTTGTG CAAATCCCCA TGGGTCAGCT GGCAAAGAGA 
AGTCTCTATC TGTCCTGCCT TCTAGAGCCC AGCCAAATAC TCTCTGAGAC CCCACTGCAA 
GGCCCTATTG CAACGGCATC ACGTTCGGAC ACCCCACTCC GTCAAGACGC GAAAGGAACC 
TTTACGACCC CCGACAACCT TCTTACTCCG AGCAGAGTGG GGAAGTTGGC TCTCGATGAT 
CCCGCTGTGA GTGTCATCAA GAACATTACC AGAACAAAGC GGGTTATGGA TTCACAACAG 
GACTCTGACC GTGCAAGTAG CTATCAGCGG CCGGGCACAG TCTCACATCA GGCTGCTGCC 
ATAGCGCCAC ATCCACCATC GCAGTCGGGT AAACCTCATC TGAAGAAAAA GAAACCCCCA 
TCAAGCAATG GTGAAAATCC TGGCCAGTCT GTTCAGGCTC CAAGTGTCCG GAAGCTTACA 
AAGCCCAGCA GGTTTGACAA GAAGTACCAA TTGTTTGGGG AGAACAGAGT CGAATCAGAC 
AACTTCAAGG GAATTCTCAC CTGTATACTG TGGGAGAGCA ATGACACCTT ACTGTACCTG 
GCTGAGGAGT TCTATAAGAA GAAGGACAGG CGGAACATTG GCCGGACAGA TCTTCTTCAA 
GAGACGTTTG AGGAGTGTGC AGATACGCTG GTGTTTAAAC TGCAGTCCTA CGAGAAGCAG 
GCCCTGGAGT ATCAGAACAA CTGCCTGCTG GAGTTCCGGG AGCAGCTACA AACTTTCGAG 
AGGCTGGTAA ATGAGGTGCC GCCGCTGGTC ATAGAGAGTC ACCGGAGGCA ACACCTGGAC 
AAACTGCTGG CGGCCACTGA CCAGATACGG AAGCAGTTTG CTGAGGACCT GCAGCGCTGG 
GACCAAGCCA AGGAGACACT GAAGAGTGTG CTGAGGCCAA ATTTAGGCCA CCCAGACAGC 
GAGCAGGCCC TGGGGGAGCT GTGCCAGCAG GAGGAACTAA GGCAGAGAGA GGAGGCAGAA 
GGGATTGACT GCAACACTAA ACAATTACAA GCGTGCGTCT CCGAGGGAAT GGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824168.1
CDS	168..4364
Translation	MAAVGQIQAVPSGRVYRQLFDAEVQLVRSLGEARARAVQHGGAVDTGNIPPIRSRDNNTALPESHHQSTDKMTPPARKKQESEDDIAAREVRGLSDVIVPEKKDTGILERIAANRLERHEVALSNLHRELAEISKEMELSVLEVGKHLQQKISESDANIELLFQKLEIGNDSTTTKEINETWDAVTQESARTRQCIKRTESTLLALEENRAKRISEVLKKYTSLLKDICHLMPSDVDRFIHNEAMMINQAILANHRALARLSVNLLEANLKRENTQRFQWQDLKKAWECYQREIIMKEFRVFAEKEMEHIPTSVKVLVDQLTTQLSSLKERRLQILGLARSFVPPTCTRTGVMEWYESLAALNSQAESINKQFLEKLQELREEFCQKFEVKAESCKEQLVSVDICTPEEAEDIITKELSPVTAQIHMKFKEEQEKWEKLLVHSSKLTILQTQKLLKFLEDVVDIWQTLERGLSKQENAVQLRMEQCRYKYDSDSKAKEANLDKILDRLRQDSTKETLRSTLGKALASLETIKAGYKTFHLDQVAVIDSYPALVLAELASYSCALSNYFGVKEVYGQVQKKDGENPSTSITTIPADGVRSHSREGISVNSEFNSYNYSAVESVLLEGEWLEGPGDGTHNTREDTEVPGIVQCDSTMAPVQTPYRESTQAAEGAGEQTSQGAQIPFELFTTSKGNTYTALRLASEDDTAVPEFPGEHSDTNIFMTEAVLDDSVPLNHDIMVIPEQQLIVIRKSVRLGFFEHLETWFGETLSSAQGIVSAKKEEFNSELALRYHLHEPRSQRIEMDIHNVRAAELILHSERVDRHCDGVSHSLEQLKRESSVLIENMKQQTLGFRSKIRAMESMFLGANKSDKLVALTQSLPSILDSHVSGVQTSMRNYRQHVEEMLGTLRDTNSDFIKSFRLFSEGGNFSPDEVEVFRKKLHKASDTIASFEGSIMVDLEGLESVCLEQATEIVKKFEDKFNMLTTDAIFLENIQKLLTNLQVKIKALVVSSNFQTQQINSSLEQLQQKTDACAHPNMDKETVTAEELYSFVQIPMGQLAKRSLYLSCLLEPSQILSETPLQGPIATASRSDTPLRQDAKGTFTTPDNLLTPSRVGKLALDDPAVSVIKNITRTKRVMDSQQDSDRASSYQRPGTVSHQAAAIAPHPPSQSGKPHLKKKKPPSSNGENPGQSVQAPSVRKLTKPSRFDKKYQLFGENRVESDNFKGILTCILWESNDTLLYLAEEFYKKKDRRNIGRTDLLQETFEECADTLVFKLQSYEKQALEYQNNCLLEFREQLQTFERLVNEVPPLVIESHRRQHLDKLLAATDQIRKQFAEDLQRWDQAKETLKSVLRPNLGHPDSEQALGELCQQEELRQREEAEGIDCNTKQLQACVSEGMG

Target ORF information:

RefSeq Version	XM_012968714.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis coiled-coil domain containing 180 (ccdc180), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968714.2

ORF Insert Sequence:

ATGGCAGCAG TGGGACAGAT CCAGGCAGTT CCTAGTGGAA GAGTCTACAG GCAACTTTTT 
GATGCTGAGG TTCAGCTTGT GCGGTCCCTC GGAGAGGCTC GTGCTAGAGC GGTGCAGCAT 
GGAGGTGCAG TGGACACTGG GAACATTCCT CCAATCAGAA GCCGGGATAA CAACACAGCA 
TTGCCTGAGT CTCACCACCA GAGCACGGAT AAAATGACTC CACCTGCTAG GAAAAAGCAG 
GAAAGTGAGG ATGATATTGC AGCAAGAGAA GTGAGAGGTC TTTCTGATGT CATCGTTCCA 
GAAAAGAAAG ATACCGGCAT TCTTGAGCGG ATCGCTGCAA ACAGACTGGA ACGCCATGAA 
GTGGCACTGT CCAACCTGCA CAGGGAACTG GCTGAGATCA GCAAAGAGAT GGAGCTATCA 
GTATTGGAAG TGGGGAAGCA CCTGCAGCAA AAGATCTCGG AATCAGATGC AAATATTGAA 
CTTTTGTTTC AGAAACTGGA AATCGGTAAC GACAGCACCA CCACAAAGGA AATAAATGAG 
ACTTGGGATG CAGTGACCCA GGAATCTGCC CGTACAAGGC AGTGCATCAA AAGAACCGAA 
AGCACTTTAC TGGCGCTAGA AGAAAACAGA GCGAAACGAA TTTCTGAAGT CTTAAAGAAA 
TACACGTCCC TCTTAAAGGA CATCTGCCAT CTCATGCCCT CCGACGTGGA CAGATTTATT 
CATAATGAAG CCATGATGAT TAACCAGGCA ATTTTAGCTA ACCACCGAGC TCTGGCTAGA 
TTATCTGTAA ACCTTCTGGA AGCCAATCTG AAGAGGGAGA ATACCCAGCG CTTCCAATGG 
CAGGACCTGA AGAAGGCCTG GGAGTGTTAC CAGAGGGAGA TCATAATGAA GGAGTTCAGG 
GTGTTTGCTG AGAAAGAAAT GGAACATATA CCCACAAGTG TGAAAGTATT AGTGGATCAG 
CTGACCACCC AACTCAGCTC TCTCAAAGAA AGGAGACTTC AGATTCTGGG TTTAGCCAGA 
AGTTTTGTAC CTCCTACTTG CACGAGAACA GGTGTCATGG AATGGTATGA ATCGTTGGCA 
GCCCTGAACA GTCAAGCTGA GTCCATCAAC AAACAATTCT TAGAGAAGCT TCAGGAGCTG 
CGGGAGGAAT TCTGCCAAAA ATTTGAGGTG AAAGCAGAAA GCTGTAAAGA GCAGTTGGTC 
AGTGTGGATA TTTGTACCCC AGAGGAAGCT GAGGATATAA TAACCAAAGA ACTCAGCCCA 
GTGACGGCAC AGATCCACAT GAAATTTAAG GAAGAGCAAG AGAAGTGGGA AAAACTGCTG 
GTACATAGCT CTAAGTTAAC GATCCTTCAG ACCCAGAAGT TGCTGAAATT CTTGGAAGAT 
GTTGTTGATA TTTGGCAAAC GCTGGAACGT GGCCTCTCCA AACAGGAAAA TGCTGTGCAG 
CTAAGGATGG AGCAGTGTCG CTATAAATAT GATAGTGACA GCAAGGCCAA GGAGGCAAAT 
TTGGACAAGA TACTGGACAG ACTGAGACAA GACAGCACTA AGGAAACATT ACGTAGCACA 
TTGGGGAAAG CACTGGCGTC CTTGGAAACC ATAAAAGCAG GATACAAGAC TTTTCATTTG 
GACCAAGTCG CTGTAATTGA TTCCTACCCT GCCCTGGTGC TTGCAGAGTT GGCCTCCTAC 
AGCTGCGCTC TTAGCAATTA TTTTGGGGTC AAAGAGGTGT ATGGGCAGGT GCAGAAGAAG 
GATGGGGAGA ACCCCAGCAC CAGTATAACA ACGATTCCGG CAGATGGAGT GAGGTCTCAT 
TCTAGAGAGG GGATATCAGT AAACTCCGAA TTTAATTCCT ACAACTATTC TGCAGTAGAA 
AGTGTTTTGC TGGAAGGTGA ATGGCTGGAA GGGCCAGGAG ATGGAACCCA CAACACAAGA 
GAAGACACGG AAGTGCCTGG AATAGTACAG TGTGATTCTA CTATGGCACC AGTGCAAACC 
CCTTATCGGG AGAGCACCCA AGCAGCAGAG GGAGCTGGTG AGCAGACATC ACAGGGAGCC 
CAGATTCCAT TTGAGCTCTT CACAACATCT AAAGGGAACA CCTATACTGC TTTGCGTTTG 
GCATCAGAAG ATGACACGGC GGTGCCTGAG TTTCCAGGAG AACATTCCGA CACAAATATA 
TTCATGACGG AGGCGGTGCT GGACGATTCC GTTCCCTTAA ATCATGACAT AATGGTCATA 
CCCGAGCAAC AGTTGATTGT TATTAGGAAA AGCGTCCGGC TTGGGTTCTT TGAGCACCTG 
GAGACCTGGT TCGGTGAGAC CCTTTCCAGT GCCCAGGGTA TCGTCAGTGC CAAGAAGGAA 
GAGTTCAATT CTGAGTTAGC GCTTCGCTAT CACCTGCACG AGCCAAGGAG CCAACGGATC 
GAGATGGATA TCCATAACGT CAGAGCTGCC GAGCTGATTC TGCACTCCGA GAGAGTGGAT 
CGTCACTGTG ATGGGGTAAG CCATTCTCTG GAGCAGCTGA AGCGGGAGAG TTCCGTGCTG 
ATTGAGAACA TGAAGCAACA GACTCTCGGA TTCCGCAGCA AGATCCGGGC CATGGAGAGC 
ATGTTCCTCG GTGCCAACAA GTCTGATAAG CTTGTGGCCC TGACACAGTC TCTGCCGTCC 
ATTTTGGACA GCCACGTAAG CGGGGTTCAG ACTTCCATGC GCAACTACCG GCAGCATGTA 
GAGGAAATGC TCGGAACCCT GCGCGACACA AACTCGGACT TCATCAAATC ATTCAGATTG 
TTCTCAGAGG GCGGAAACTT TTCTCCCGAT GAAGTGGAGG TGTTTCGCAA GAAGCTGCAT 
AAAGCGTCAG ACACCATCGC CTCTTTCGAG GGCTCCATCA TGGTGGATTT GGAGGGCTTG 
GAGTCTGTGT GTTTAGAACA GGCCACTGAG ATTGTAAAGA AATTTGAGGA CAAATTCAAC 
ATGTTAACAA CGGATGCAAT TTTCCTAGAA AACATTCAGA AGCTGCTGAC GAACCTGCAA 
GTGAAAATCA AAGCCTTGGT TGTGAGCAGC AATTTTCAGA CTCAGCAGAT CAACTCCTCT 
CTGGAGCAGC TCCAACAGAA GACAGATGCC TGCGCTCATC CCAATATGGA TAAGGAAACA 
GTCACAGCTG AAGAACTGTA CTCCTTTGTG CAAATCCCCA TGGGTCAGCT GGCAAAGAGA 
AGTCTCTATC TGTCCTGCCT TCTAGAGCCC AGCCAAATAC TCTCTGAGAC CCCACTGCAA 
GGCCCTATTG CAACGGCATC ACGTTCGGAC ACCCCACTCC GTCAAGACGC GAAAGGAACC 
TTTACGACCC CCGACAACCT TCTTACTCCG AGCAGAGTGG GGAAGTTGGC TCTCGATGAT 
CCCGCTGTGA GTGTCATCAA GAACATTACC AGAACAAAGC GGGTTATGGA TTCACAACAG 
GACTCTGACC GTGCAAGTAG CTATCAGCGG CCGGGCACAG TCTCACATCA GGCTGCTGCC 
ATAGCGCCAC ATCCACCATC GCAGTCGGGT AAACCTCATC TGAAGAAAAA GAAACCCCCA 
TCAAGCAATG GTGAAAATCC TGGCCAGTCT GTTCAGGCTC CAAGTGTCCG GAAGCTTACA 
AAGCCCAGCA GGTTTGACAA GAAGTACCAA TTGTTTGGGG AGAACAGAGT CGAATCAGAC 
AACTTCAAGG GAATTCTCAC CTGTATACTG TGGGAGAGCA ATGACACCTT ACTGTACCTG 
GCTGAGGAGT TCTATAAGAA GAAGGACAGG CGGAACATTG GCCGGACAGA TCTTCTTCAA 
GAGACGTTTG AGGAGTGTGC AGATACGCTG GTGTTTAAAC TGCAGTCCTA CGAGAAGCAG 
GCCCTGGAGT ATCAGAACAA CTGCCTGCTG GAGTTCCGGG AGCAGCTACA AACTTTCGAG 
AGGCTGGTAA ATGAGGTGCC GCCGCTGGTC ATAGAGAGTC ACCGGAGGCA ACACCTGGAC 
AAACTGCTGG CGGCCACTGA CCAGATACGG AAGCAGTTTG CTGAGGACCT GCAGCGCTGG 
GACCAAGCCA AGGAGACACT GAAGAGTGTG CTGAGGCCAA ATTTAGGCCA CCCAGACAGC 
GAGCAGGCCC TGGGGGAGCT GTGCCAGCAG GAGGAACTAA GGCAGAGAGA GGAGGCAGAA 
GGGATTGACT GCAACACTAA ACAATTACAA GCGTGCGTCT CCGAGGGAAT GGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ccdc180 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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