mdc1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mdc1
Entrez Gene ID	100489926
Full Name	mediator of DNA damage checkpoint 1
Synonyms	nfbd1
General protein information	Preferred Names mediator of DNA damage checkpoint 1 Names mediator of DNA damage checkpoint protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012969222.2	XP_012824676.1	mediator of DNA damage checkpoint protein 1
XM_031890790.1	XP_031746650.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_031890791.1	XP_031746651.1	mediator of DNA damage checkpoint protein 1 isoform X3
XM_012969222.3	XP_012824676.2	mediator of DNA damage checkpoint protein 1 isoform X1
XM_002939467.5	XP_002939513.3	mediator of DNA damage checkpoint protein 1 isoform X2
XM_002939467.4	XP_002939513.2	mediator of DNA damage checkpoint protein 1
XM_004916906.3	XP_004916963.1	mediator of DNA damage checkpoint protein 1

Pathways from BioSystems
Gene Ontology

The following mdc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mdc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa64006	XM_031890791.1 Latest version!	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa14440	XM_002939467.4 Latest version!	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa14440	XM_004916906.3 Latest version!	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa14440	XM_012969222.2 Latest version!	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa64004	XM_031890790.1 Latest version!	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa64004	XM_012969222.3 Latest version!	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa64005	XM_002939467.5 Latest version!	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mdc1 ( XM_031890791.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746651.1 Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa64006
Clone ID Related Accession (Same CDS sequence)	XM_031890791.1
Accession Version	XM_031890791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGATA CTCAGCGCCT GCAGTGGGAC GATGAAGAAC AAGATGTGTC TTTTGATAGA 
GATAAACCAG TAGGAAATCT GCATATGTTC AATGGAATCC ATGGTCCAGC ACAAGACTTC 
CCTATTTACC CTGGTACGAA TATCATAGGA CGTCATACAA ATTGTGATGT GACTCTTCCA 
GCTCAGTCTG TCTCTAAGAA ACATGCTATT ATAGAGGTTG AGGCTGACTG CCATACCCTC 
TGTGACCAAG GAAGTCTAAA CAAAACTCGC CGTGGCAAGG CAGCCATTGC TCCTCATGTG 
AGATATGCTC TGTCTGATGG AGACTTATTG TTATTTGCTG ATGTAGCATG TCGTTACTCA 
TTCACAAAAA AACTAGAGGA GGAGGAGGCA GAGAATGAGG AAAGTGAAGA TGATATTTTG 
GTTCCTGGAA CACAGGCTAC TTTGTCAATT GAAAAAACTC CTGGGGTAGC AATACGCAGA 
ATGGGGAGAG GAGCAGTGCT GGCAATGGAC TCTGGTGATG AGGAAGAAGA GGAATTAAAA 
GGGAAAGATC TGAATTATGA AGCTAAAGGT GAGGAAGACA ATTACATACA AGAAGGTCTT 
AAAACATCCG GGGAGGAATG CAAGTCACCT GCCAGTGCTT CCTTTTCCCA CAACATGGAT 
ACTATTGTTC CTGAAAGTGA TGAGGAGAAC GACACATCTG TGTCTGAACC ACGTTTCCCT 
TCACTCAACC TCCGTTGTGA TAGTGATACA GACACTCATG ACACTCCCAT GAAGGCAGGC 
ACAAGCTACA CCCCCTCCCA AGATTGTGTG CTTTCTCCTT CTTTCAAAGA ACAAGTTGTG 
GGAGACCACA GTGAAATCAC AAAATCTACT TTGATTAGCA ACCAAGAGAT GTTTGCAGAT 
GAGCAAGTAA ACACTGAATC CAAAGTCTCG AAGTGTAGTC CTGATACAGC TGACAAAAAT 
GATAGAGAGA CAAAGGAAGA GAACAATACT CCTATAGTAA ACACTAATAT TGCTGAGGAA 
GGTGGAACAG GAGTAACAGA TGATCCATTG ATAGACGGTC TAAAAGTGGA AAACCAAAAA 
AAGGAAAATG ACTGTTATGA AACCCTGCAA GTTGACATCA AACAGCAAGA GAGTGTTAAA 
ATTATCTTGG ATAGTAATCA GGAGGAAAAC AGAACTTTGA AAGTAAAATT GGTAAACACA 
GGATCTGCAG ATATTATTCT AGACAAAAAT ATAGATGGGG AAGAAGGTTG CGTCAATACT 
AAGGAAATAG ACAACCAGAT CAGAGAAGGG GCTTTTCATT TGGACAGTGA CACAGATGTA 
GAGGAGAGCG ATACTTCAAT CCCTGGAATA AATGTACCCC GAGTGGAGAA TTCTGGGAAT 
AACACAGACA GTGATACAGA TACTGAAGAA GATAAACCTA AAATTTTGCA ACCTGCCATA 
CAACAGAATG TCGAGGAAGG GTTTAATTTG GACAGTGACA CGGATGTTGA GGGTAATGAC 
TCCAATTCTG TAAATAAAAG GTTGAAAAAT ACTGAAAAAG CAAAAATGAT CCAAGATAGT 
GATACTGATG CGGATGATTC GATTATGTCA AAGGTTGAAG AGAGGAGGAA CCCAGCAGGT 
ACAAAGAAAG ATTACAGTAG TACATCTCTA AACACAGAAG GTAAAAGGCA AGAAAATTAT 
CTATCCACCC TTGACAGTGA TACAGATGTT GAAGACATTG CTGATGGATC TTCCCCAAGT 
GTTGTGAAGA AGCAGGATAA GGCAGAAGCA GCTCATACTA ATGTAACCGA TACAACTGCT 
GGGGAGGAAA CTTGCACAGA TGCTGGAATA GAGAAGATTG TGGTGTCTGC AGATAGAAAT 
GAATGCCACA CAGGTGGAGT CACACAAGAA CAGGAGGCTA CATCTGAATT TCACCTAGAC 
AGTGATACAG ATATTGAGGA AGATGATAAT TTACTAGATA TGTCAAGTGC TAGTACTAGG 
GTAAAAGTCA CAGAGGTTCA CTCAGTTATC CTTGAAAAAC TAAGAACCTC ACCTGGAGAA 
AGAAAGGAAA AGAGGGCTAT TGAAATTGAT CTGGACAGCA ATACAGATGT GGATGAAGCT 
GATGAAACAG AAGTTGATAG GAAGGAAACA TTTCACATGG ACAGTGATAC AGATCTGGAG 
GAAAATGACA GTGGCCCTCC TGTGGTGGGT GGCATAGAAA AGAAATTTAC AGCCATAGCA 
CATTCTGATT GTACCTCAGA CACAGGAAGT AATGTGAAAA ATGTAACCTC TTCAATCACA 
GAAAATACAG AAAAAGGGGA GACTGATGGA ATAGACTGCG AAGATGCAGA ATATCAAATG 
ATGGCTACTC AGTGTTATCT AGAGCCCCAG GAAAAAGAGA CTGATATACA AGATGAAGAT 
GATTGTGCTG AGGAAGCTAC ACAAGCATTT ATCTTCAGTT CCACATGGGC TGAGCCGGAA 
CCATTTAAAC GTCCAGCCGA TCCTATTGGA GTTTTGCAAA TCTCAGCAGT GACTGTTAGT 
TCATCAGAGG AAGAACCAGA TGAAAATGCC ATTGCGGAGA CTCAGCCATT CTGCTGTGAG 
ATAAAGCAGC CAGAGGAACA CAGTGACCAA GAGCCAGCCA AAAAAAGTTC TATTGCTGAG 
GAAAAGAACA ATGTAGCTTG CTCTCTGCCT CAAGAAGAAA TATCTCAGGA TGATACCCAG 
CCAGTGTCTC AGTACTTGAA TAAAACAGCT TCAAGTAACT TGGTGGCACC AAATTTAACT 
TGCACAACAA ATTTTCAACA AGAAAAATAT CTTGATGGAA TTAATGAAGA AAAGGTCAAA 
CAGGCTAATG AAAGTGTTCC AAACAGGGAG GAACAGCTTG ATGAAGGTGT CCAAGACATC 
GAAGAGGATG CCACCCAACC ATATGTTTTA AGTTTACCGA CAGTGGGTGA CATGGCTACA 
CAGCCTTGTA GCATGAGTGA GTCTGTCTCC GGGGATGGTG CTAGTCAGTA TTTAAATACA 
GTTGTTCCAG CTGTAGCTAG TGTTGCTTCT CTACCCTGTA ACCCAAGCAT GCAATTATCT 
GATGGCGAAC CCATGCAGCC TTCCAGTCTA AATGAGGCTG ATGCAACCCA ATGTCATGGA 
TTTAGTATGC CCCTTAAAGA AGGTGATACA GTGAGCATTT CAGAAGAGAA AGTATCAGTA 
AAGCAAGGTC CTTGCAGAGG TGAGAGTAGC ATGATATCTG AGCAGTTAGA CCCTTCTAAC 
AATGTTATGA GCAAGCACCC AGGTGAAGTT CTTGTGCATT CTGTTCCTCC ATTGGAGAAA 
GCAACAGAGC AGGTTGCAGA GCAGCCTCAA GCAGCCACCA GAATGACAGA AGAAGACAGA 
GAAGAGAATA AAGGAAAGAG GAGATCAACA GAAGGAGAAG ATAGCAAGGA TTCAGCAGAA 
GTTACAACTT CCGTTACTAG GGGTAAAAAG AGAACCTGGA AAAAGACTGC ATCTCCAGCA 
TCAGAGGAGA CAAAAGGCAA AAAAACAGCT AAAATGCAGG TCATGGAAAA AGCATTACCT 
GAAGCAAAAA TGGAAGAAGA ATGTAAAGAA GAGCAGAACA ATAAGCCAAA CACTAGCTCT 
ATCCTTGCAC GTTTAAAAAC AGGTAGAAAG GGAAAACAAG ATATCGAAGA GCCTTTAGAT 
TACAATGTAG AAGGCTATAA TGTGAGCGTG CAACAGGTAG AAGAGGATAT GAAAAGATTT 
ACTTCAAGAA GATCAGTTAG AAAACCAAAG GATGGAAATG ATCAGGAGAC TGGGTCAAGA 
AATTTACCAG AAATAACCAG GCATTCCAGT TTAGACCTTA GTGAGTCAGT AGTGGAAAAA 
AAAGTAGATA TGTCTTCTGA TGAACACATG AAGTATCAGA AAGATGATGA ATTCTTAACT 
GCTAATGGAA CAACTATAAA TGTAAAAAGA AAGGGTGCAA AGAGAAATGT AAAGGAAGAG 
GAGAACAAGT TTGTGGTAGA TAACATTCCT GGAAAGCGAC CAACAAGGAA TAACAGGCCA 
AATGTTACAG AGAAAAATAA TGAGGAAGCA GATGATAAAC AGAGGATTAC AAGGAGGAAT 
TCAAGGCAGC TGGAGATAGT AGAAATCAAA CCAGACAATA CAGAGATTTT AGATTCTAAA 
AGACAAAACC AGTCCAGAAA ATCACCAAAT ATAAAGAAAA ATTTAAAAGT GGATGAATCA 
AAAGAGACAG AAAAATGTGA GGGCAAAACT AGAGAGAACA CTCCGAAAAG GACATCAAGG 
AGGACAAAGA AAAAATGCAC AGAGGAACTG AACATGGAAG AATGGGAAAA GTCAAAAGAA 
GAAGCAGACA GTAAACAGAG GGTCACAAGA AGGAACTCCA GGCTATCAGA GTCAGTAGAA 
AGCATACCAG AGGATACAGA TATTGTAGAT TCTGAGCAAC AAGACCAGTC CAGAAAATCT 
CGTAATATAA AGAAGAATTT GAAAAGGGGC GAGGAAGAAA AACTTGAGGG CACCAACAGA 
GACAACACTG AAAAATGGAC CTCAAGGAGG ACAAGAAAGG AATGCAAAGA GAAACTGAAC 
ATGGAGGACC ATGAAAAGTC GAAAGTAGAA CCATCCATTG AACAGTCTGA ACAAGAACAT 
ACTGAGGCTG AAGGACAAGA TCAATCTAGA AAATCTAGGA AGACAAAGAA GGATTTGCCA 
CAACCTGTTA TCAATGAGGA AGAAACCCTT GTAGAAACCA TCAGGAAAGA CTCTGTAGGA 
CGAACATCAA GGAGAAGAAA GAAGAGTTGC ATAGAGGAAG ATCTGATGGA CAGTGAGGTT 
CTAGTTATCA GTAAACCTGA ACAAGAAGAT CCACCCACAA AAAATGTTCC TAGAAGTAAA 
AGGAAGAATT TGAAAGATGA ACCTAAGGAG GGAGATGGTA GCATGACAAT AGACTCGCAC 
GAAGACGTTT CACCAACTGG AATGAGAAAA AGCAGGCGGA CAACAACCAA GGAGGAATTA 
CTCAAGTCTA CCACTCTGGC TCCAGTAAGG AAGAGAGGGC AACAGCCAAA AACTGGAGGA 
GATGAGGTCA AGAGAAAAAA AGTGAATGAA GAGTTTGAGC AGGAGAAAAC AGTGGGTAGA 
AGGGGCCAAG CACGGCAACC AAAGCTGGAG GATGAAAATA CTGACGGCAA CAGTTTTGGA 
AAGGACTCGG CCGTTCCTTT TCCATCACCC TCGGGGAACT CCCGTTTAAG GCCAAGCACA 
TCCTTTGAAA GCCCGACAGA AGGCCGGACA CCAAGACGTG CTGCTCGCAG CTTGACCACA 
AGCCCCTACA TGTCCCAACG GAGCATTGCA GCAAAGGTTC TATTTACAGG TGTGGTCGAC 
CCTGCAGGTG AGGAGACCAT TCGTAATCTA GGAGGGGAAG TTGCAGAATC CATATTTGAC 
TGCACTCATC TTGTAACTGA CCGTATCAGA CGTACCGTCA AATTTCTCTG TGCTCTTGCC 
AAGGGCATCC CTATTGTGAC ACTAGACTGG CTTGACAAGT GCAAAAAGAG CAGATGTTTT 
TTGTCCCCTG CTCAATTTCT GGTGAACGAT AAAGAACAGG AGAAAAGCTT CAACTTCGAT 
TTGTCAGAAT CATTGCAAAA AGCCAAAAAG AAACTGCTGT TTGAGGGCTA TGAAATTCAT 
GTCACCCCGA ATGTGAAACC TGAGCCTGAA CACATGAAAG ATATTATACA GTGCAGTGGG 
GCAACTTTCC TTCCTAAAAT GCCCAAAGTA TACAAGGACA AATGCATCAT TGTATCATGT 
AAGGAAGATG CCACTCGCTG CAAATCTGCT CCCTCCACTA TCCCTGTTAC TTCTGCGGAA 
TTTATATTAA CTGGGATCCT GCGTCAGGAA ATAAATCCAC ATGCTTATCT GCTAAGTACT 
GGACCTGAGG AAGCTTTGCC TACCCCTGCC AAACGGAGGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746651.1
CDS	232..6156
Translation	MDDTQRLQWDDEEQDVSFDRDKPVGNLHMFNGIHGPAQDFPIYPGTNIIGRHTNCDVTLPAQSVSKKHAIIEVEADCHTLCDQGSLNKTRRGKAAIAPHVRYALSDGDLLLFADVACRYSFTKKLEEEEAENEESEDDILVPGTQATLSIEKTPGVAIRRMGRGAVLAMDSGDEEEEELKGKDLNYEAKGEEDNYIQEGLKTSGEECKSPASASFSHNMDTIVPESDEENDTSVSEPRFPSLNLRCDSDTDTHDTPMKAGTSYTPSQDCVLSPSFKEQVVGDHSEITKSTLISNQEMFADEQVNTESKVSKCSPDTADKNDRETKEENNTPIVNTNIAEEGGTGVTDDPLIDGLKVENQKKENDCYETLQVDIKQQESVKIILDSNQEENRTLKVKLVNTGSADIILDKNIDGEEGCVNTKEIDNQIREGAFHLDSDTDVEESDTSIPGINVPRVENSGNNTDSDTDTEEDKPKILQPAIQQNVEEGFNLDSDTDVEGNDSNSVNKRLKNTEKAKMIQDSDTDADDSIMSKVEERRNPAGTKKDYSSTSLNTEGKRQENYLSTLDSDTDVEDIADGSSPSVVKKQDKAEAAHTNVTDTTAGEETCTDAGIEKIVVSADRNECHTGGVTQEQEATSEFHLDSDTDIEEDDNLLDMSSASTRVKVTEVHSVILEKLRTSPGERKEKRAIEIDLDSNTDVDEADETEVDRKETFHMDSDTDLEENDSGPPVVGGIEKKFTAIAHSDCTSDTGSNVKNVTSSITENTEKGETDGIDCEDAEYQMMATQCYLEPQEKETDIQDEDDCAEEATQAFIFSSTWAEPEPFKRPADPIGVLQISAVTVSSSEEEPDENAIAETQPFCCEIKQPEEHSDQEPAKKSSIAEEKNNVACSLPQEEISQDDTQPVSQYLNKTASSNLVAPNLTCTTNFQQEKYLDGINEEKVKQANESVPNREEQLDEGVQDIEEDATQPYVLSLPTVGDMATQPCSMSESVSGDGASQYLNTVVPAVASVASLPCNPSMQLSDGEPMQPSSLNEADATQCHGFSMPLKEGDTVSISEEKVSVKQGPCRGESSMISEQLDPSNNVMSKHPGEVLVHSVPPLEKATEQVAEQPQAATRMTEEDREENKGKRRSTEGEDSKDSAEVTTSVTRGKKRTWKKTASPASEETKGKKTAKMQVMEKALPEAKMEEECKEEQNNKPNTSSILARLKTGRKGKQDIEEPLDYNVEGYNVSVQQVEEDMKRFTSRRSVRKPKDGNDQETGSRNLPEITRHSSLDLSESVVEKKVDMSSDEHMKYQKDDEFLTANGTTINVKRKGAKRNVKEEENKFVVDNIPGKRPTRNNRPNVTEKNNEEADDKQRITRRNSRQLEIVEIKPDNTEILDSKRQNQSRKSPNIKKNLKVDESKETEKCEGKTRENTPKRTSRRTKKKCTEELNMEEWEKSKEEADSKQRVTRRNSRLSESVESIPEDTDIVDSEQQDQSRKSRNIKKNLKRGEEEKLEGTNRDNTEKWTSRRTRKECKEKLNMEDHEKSKVEPSIEQSEQEHTEAEGQDQSRKSRKTKKDLPQPVINEEETLVETIRKDSVGRTSRRRKKSCIEEDLMDSEVLVISKPEQEDPPTKNVPRSKRKNLKDEPKEGDGSMTIDSHEDVSPTGMRKSRRTTTKEELLKSTTLAPVRKRGQQPKTGGDEVKRKKVNEEFEQEKTVGRRGQARQPKLEDENTDGNSFGKDSAVPFPSPSGNSRLRPSTSFESPTEGRTPRRAARSLTTSPYMSQRSIAAKVLFTGVVDPAGEETIRNLGGEVAESIFDCTHLVTDRIRRTVKFLCALAKGIPIVTLDWLDKCKKSRCFLSPAQFLVNDKEQEKSFNFDLSESLQKAKKKLLFEGYEIHVTPNVKPEPEHMKDIIQCSGATFLPKMPKVYKDKCIIVSCKEDATRCKSAPSTIPVTSAEFILTGILRQEINPHAYLLSTGPEEALPTPAKRRR

Target ORF information:

RefSeq Version	XM_031890791.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890791.1

ORF Insert Sequence:

ATGGACGATA CTCAGCGCCT GCAGTGGGAC GATGAAGAAC AAGATGTGTC TTTTGATAGA 
GATAAACCAG TAGGAAATCT GCATATGTTC AATGGAATCC ATGGTCCAGC ACAAGACTTC 
CCTATTTACC CTGGTACGAA TATCATAGGA CGTCATACAA ATTGTGATGT GACTCTTCCA 
GCTCAGTCTG TCTCTAAGAA ACATGCTATT ATAGAGGTTG AGGCTGACTG CCATACCCTC 
TGTGACCAAG GAAGTCTAAA CAAAACTCGC CGTGGCAAGG CAGCCATTGC TCCTCATGTG 
AGATATGCTC TGTCTGATGG AGACTTATTG TTATTTGCTG ATGTAGCATG TCGTTACTCA 
TTCACAAAAA AACTAGAGGA GGAGGAGGCA GAGAATGAGG AAAGTGAAGA TGATATTTTG 
GTTCCTGGAA CACAGGCTAC TTTGTCAATT GAAAAAACTC CTGGGGTAGC AATACGCAGA 
ATGGGGAGAG GAGCAGTGCT GGCAATGGAC TCTGGTGATG AGGAAGAAGA GGAATTAAAA 
GGGAAAGATC TGAATTATGA AGCTAAAGGT GAGGAAGACA ATTACATACA AGAAGGTCTT 
AAAACATCCG GGGAGGAATG CAAGTCACCT GCCAGTGCTT CCTTTTCCCA CAACATGGAT 
ACTATTGTTC CTGAAAGTGA TGAGGAGAAC GACACATCTG TGTCTGAACC ACGTTTCCCT 
TCACTCAACC TCCGTTGTGA TAGTGATACA GACACTCATG ACACTCCCAT GAAGGCAGGC 
ACAAGCTACA CCCCCTCCCA AGATTGTGTG CTTTCTCCTT CTTTCAAAGA ACAAGTTGTG 
GGAGACCACA GTGAAATCAC AAAATCTACT TTGATTAGCA ACCAAGAGAT GTTTGCAGAT 
GAGCAAGTAA ACACTGAATC CAAAGTCTCG AAGTGTAGTC CTGATACAGC TGACAAAAAT 
GATAGAGAGA CAAAGGAAGA GAACAATACT CCTATAGTAA ACACTAATAT TGCTGAGGAA 
GGTGGAACAG GAGTAACAGA TGATCCATTG ATAGACGGTC TAAAAGTGGA AAACCAAAAA 
AAGGAAAATG ACTGTTATGA AACCCTGCAA GTTGACATCA AACAGCAAGA GAGTGTTAAA 
ATTATCTTGG ATAGTAATCA GGAGGAAAAC AGAACTTTGA AAGTAAAATT GGTAAACACA 
GGATCTGCAG ATATTATTCT AGACAAAAAT ATAGATGGGG AAGAAGGTTG CGTCAATACT 
AAGGAAATAG ACAACCAGAT CAGAGAAGGG GCTTTTCATT TGGACAGTGA CACAGATGTA 
GAGGAGAGCG ATACTTCAAT CCCTGGAATA AATGTACCCC GAGTGGAGAA TTCTGGGAAT 
AACACAGACA GTGATACAGA TACTGAAGAA GATAAACCTA AAATTTTGCA ACCTGCCATA 
CAACAGAATG TCGAGGAAGG GTTTAATTTG GACAGTGACA CGGATGTTGA GGGTAATGAC 
TCCAATTCTG TAAATAAAAG GTTGAAAAAT ACTGAAAAAG CAAAAATGAT CCAAGATAGT 
GATACTGATG CGGATGATTC GATTATGTCA AAGGTTGAAG AGAGGAGGAA CCCAGCAGGT 
ACAAAGAAAG ATTACAGTAG TACATCTCTA AACACAGAAG GTAAAAGGCA AGAAAATTAT 
CTATCCACCC TTGACAGTGA TACAGATGTT GAAGACATTG CTGATGGATC TTCCCCAAGT 
GTTGTGAAGA AGCAGGATAA GGCAGAAGCA GCTCATACTA ATGTAACCGA TACAACTGCT 
GGGGAGGAAA CTTGCACAGA TGCTGGAATA GAGAAGATTG TGGTGTCTGC AGATAGAAAT 
GAATGCCACA CAGGTGGAGT CACACAAGAA CAGGAGGCTA CATCTGAATT TCACCTAGAC 
AGTGATACAG ATATTGAGGA AGATGATAAT TTACTAGATA TGTCAAGTGC TAGTACTAGG 
GTAAAAGTCA CAGAGGTTCA CTCAGTTATC CTTGAAAAAC TAAGAACCTC ACCTGGAGAA 
AGAAAGGAAA AGAGGGCTAT TGAAATTGAT CTGGACAGCA ATACAGATGT GGATGAAGCT 
GATGAAACAG AAGTTGATAG GAAGGAAACA TTTCACATGG ACAGTGATAC AGATCTGGAG 
GAAAATGACA GTGGCCCTCC TGTGGTGGGT GGCATAGAAA AGAAATTTAC AGCCATAGCA 
CATTCTGATT GTACCTCAGA CACAGGAAGT AATGTGAAAA ATGTAACCTC TTCAATCACA 
GAAAATACAG AAAAAGGGGA GACTGATGGA ATAGACTGCG AAGATGCAGA ATATCAAATG 
ATGGCTACTC AGTGTTATCT AGAGCCCCAG GAAAAAGAGA CTGATATACA AGATGAAGAT 
GATTGTGCTG AGGAAGCTAC ACAAGCATTT ATCTTCAGTT CCACATGGGC TGAGCCGGAA 
CCATTTAAAC GTCCAGCCGA TCCTATTGGA GTTTTGCAAA TCTCAGCAGT GACTGTTAGT 
TCATCAGAGG AAGAACCAGA TGAAAATGCC ATTGCGGAGA CTCAGCCATT CTGCTGTGAG 
ATAAAGCAGC CAGAGGAACA CAGTGACCAA GAGCCAGCCA AAAAAAGTTC TATTGCTGAG 
GAAAAGAACA ATGTAGCTTG CTCTCTGCCT CAAGAAGAAA TATCTCAGGA TGATACCCAG 
CCAGTGTCTC AGTACTTGAA TAAAACAGCT TCAAGTAACT TGGTGGCACC AAATTTAACT 
TGCACAACAA ATTTTCAACA AGAAAAATAT CTTGATGGAA TTAATGAAGA AAAGGTCAAA 
CAGGCTAATG AAAGTGTTCC AAACAGGGAG GAACAGCTTG ATGAAGGTGT CCAAGACATC 
GAAGAGGATG CCACCCAACC ATATGTTTTA AGTTTACCGA CAGTGGGTGA CATGGCTACA 
CAGCCTTGTA GCATGAGTGA GTCTGTCTCC GGGGATGGTG CTAGTCAGTA TTTAAATACA 
GTTGTTCCAG CTGTAGCTAG TGTTGCTTCT CTACCCTGTA ACCCAAGCAT GCAATTATCT 
GATGGCGAAC CCATGCAGCC TTCCAGTCTA AATGAGGCTG ATGCAACCCA ATGTCATGGA 
TTTAGTATGC CCCTTAAAGA AGGTGATACA GTGAGCATTT CAGAAGAGAA AGTATCAGTA 
AAGCAAGGTC CTTGCAGAGG TGAGAGTAGC ATGATATCTG AGCAGTTAGA CCCTTCTAAC 
AATGTTATGA GCAAGCACCC AGGTGAAGTT CTTGTGCATT CTGTTCCTCC ATTGGAGAAA 
GCAACAGAGC AGGTTGCAGA GCAGCCTCAA GCAGCCACCA GAATGACAGA AGAAGACAGA 
GAAGAGAATA AAGGAAAGAG GAGATCAACA GAAGGAGAAG ATAGCAAGGA TTCAGCAGAA 
GTTACAACTT CCGTTACTAG GGGTAAAAAG AGAACCTGGA AAAAGACTGC ATCTCCAGCA 
TCAGAGGAGA CAAAAGGCAA AAAAACAGCT AAAATGCAGG TCATGGAAAA AGCATTACCT 
GAAGCAAAAA TGGAAGAAGA ATGTAAAGAA GAGCAGAACA ATAAGCCAAA CACTAGCTCT 
ATCCTTGCAC GTTTAAAAAC AGGTAGAAAG GGAAAACAAG ATATCGAAGA GCCTTTAGAT 
TACAATGTAG AAGGCTATAA TGTGAGCGTG CAACAGGTAG AAGAGGATAT GAAAAGATTT 
ACTTCAAGAA GATCAGTTAG AAAACCAAAG GATGGAAATG ATCAGGAGAC TGGGTCAAGA 
AATTTACCAG AAATAACCAG GCATTCCAGT TTAGACCTTA GTGAGTCAGT AGTGGAAAAA 
AAAGTAGATA TGTCTTCTGA TGAACACATG AAGTATCAGA AAGATGATGA ATTCTTAACT 
GCTAATGGAA CAACTATAAA TGTAAAAAGA AAGGGTGCAA AGAGAAATGT AAAGGAAGAG 
GAGAACAAGT TTGTGGTAGA TAACATTCCT GGAAAGCGAC CAACAAGGAA TAACAGGCCA 
AATGTTACAG AGAAAAATAA TGAGGAAGCA GATGATAAAC AGAGGATTAC AAGGAGGAAT 
TCAAGGCAGC TGGAGATAGT AGAAATCAAA CCAGACAATA CAGAGATTTT AGATTCTAAA 
AGACAAAACC AGTCCAGAAA ATCACCAAAT ATAAAGAAAA ATTTAAAAGT GGATGAATCA 
AAAGAGACAG AAAAATGTGA GGGCAAAACT AGAGAGAACA CTCCGAAAAG GACATCAAGG 
AGGACAAAGA AAAAATGCAC AGAGGAACTG AACATGGAAG AATGGGAAAA GTCAAAAGAA 
GAAGCAGACA GTAAACAGAG GGTCACAAGA AGGAACTCCA GGCTATCAGA GTCAGTAGAA 
AGCATACCAG AGGATACAGA TATTGTAGAT TCTGAGCAAC AAGACCAGTC CAGAAAATCT 
CGTAATATAA AGAAGAATTT GAAAAGGGGC GAGGAAGAAA AACTTGAGGG CACCAACAGA 
GACAACACTG AAAAATGGAC CTCAAGGAGG ACAAGAAAGG AATGCAAAGA GAAACTGAAC 
ATGGAGGACC ATGAAAAGTC GAAAGTAGAA CCATCCATTG AACAGTCTGA ACAAGAACAT 
ACTGAGGCTG AAGGACAAGA TCAATCTAGA AAATCTAGGA AGACAAAGAA GGATTTGCCA 
CAACCTGTTA TCAATGAGGA AGAAACCCTT GTAGAAACCA TCAGGAAAGA CTCTGTAGGA 
CGAACATCAA GGAGAAGAAA GAAGAGTTGC ATAGAGGAAG ATCTGATGGA CAGTGAGGTT 
CTAGTTATCA GTAAACCTGA ACAAGAAGAT CCACCCACAA AAAATGTTCC TAGAAGTAAA 
AGGAAGAATT TGAAAGATGA ACCTAAGGAG GGAGATGGTA GCATGACAAT AGACTCGCAC 
GAAGACGTTT CACCAACTGG AATGAGAAAA AGCAGGCGGA CAACAACCAA GGAGGAATTA 
CTCAAGTCTA CCACTCTGGC TCCAGTAAGG AAGAGAGGGC AACAGCCAAA AACTGGAGGA 
GATGAGGTCA AGAGAAAAAA AGTGAATGAA GAGTTTGAGC AGGAGAAAAC AGTGGGTAGA 
AGGGGCCAAG CACGGCAACC AAAGCTGGAG GATGAAAATA CTGACGGCAA CAGTTTTGGA 
AAGGACTCGG CCGTTCCTTT TCCATCACCC TCGGGGAACT CCCGTTTAAG GCCAAGCACA 
TCCTTTGAAA GCCCGACAGA AGGCCGGACA CCAAGACGTG CTGCTCGCAG CTTGACCACA 
AGCCCCTACA TGTCCCAACG GAGCATTGCA GCAAAGGTTC TATTTACAGG TGTGGTCGAC 
CCTGCAGGTG AGGAGACCAT TCGTAATCTA GGAGGGGAAG TTGCAGAATC CATATTTGAC 
TGCACTCATC TTGTAACTGA CCGTATCAGA CGTACCGTCA AATTTCTCTG TGCTCTTGCC 
AAGGGCATCC CTATTGTGAC ACTAGACTGG CTTGACAAGT GCAAAAAGAG CAGATGTTTT 
TTGTCCCCTG CTCAATTTCT GGTGAACGAT AAAGAACAGG AGAAAAGCTT CAACTTCGAT 
TTGTCAGAAT CATTGCAAAA AGCCAAAAAG AAACTGCTGT TTGAGGGCTA TGAAATTCAT 
GTCACCCCGA ATGTGAAACC TGAGCCTGAA CACATGAAAG ATATTATACA GTGCAGTGGG 
GCAACTTTCC TTCCTAAAAT GCCCAAAGTA TACAAGGACA AATGCATCAT TGTATCATGT 
AAGGAAGATG CCACTCGCTG CAAATCTGCT CCCTCCACTA TCCCTGTTAC TTCTGCGGAA 
TTTATATTAA CTGGGATCCT GCGTCAGGAA ATAAATCCAC ATGCTTATCT GCTAAGTACT 
GGACCTGAGG AAGCTTTGCC TACCCCTGCC AAACGGAGGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdc1 ( XM_012969222.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824676.1 Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X3, mRNA.

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CloneID	OXa14440
Clone ID Related Accession (Same CDS sequence)	XM_012969222.2 , XM_002939467.4 , XM_004916906.3
Accession Version	XM_012969222.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969222.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA TTGTTCCTGA AAGTGATGAG GAGAACGACA CATCTGTGTC TGAACCACGT 
TTCCCTTCAC TCAACCTCCG TTGTGATAGT GATACAGACA CTCATGACAC TCCCATGAAG 
GCAGGCACAA GCTACACCCC CTCCCAAGAT TGTGTGCTTT CTCCTTCTTT CAAAGAACAA 
GTTGTGGGAG ACCACAGTGA AATCACAAAA TCTACTTTGA TTAGCAACCA AGAGATGTTT 
GCAGATGAGC AAGTAAACAC TGAATCCAAA GTCTCGAAGT GTAGTCCTGA TACAGCTGAC 
AAAAATGATA GAGAGACAAA GGAAGAGAAC AATACTCCTA TAGTAAACAC TAATATTGCT 
GAGGAAGGTG GAACAGGAGT AACAGATGAT CCATTGATAG ACGGTCTAAA AGTGGAAAAC 
CAAAAAAAGG AAAATGACTG TTATGAAACC CTGCAAGTTG ACATCAAACA GCAAGAGAGT 
GTTAAAATTA TCTTGGATAG TAATCAGGAG GAAAACAGAA CTTTGAAAGT AAAATTGGTA 
AACACAGGAT CTGCAGATAT TATTCTAGAC AAAAATATAG ATGGGGAAGA AGGTTGCGTC 
AATACTAAGG AAATAGACAA CCAGATCAGA GAAGGGGCTT TTCATTTGGA CAGTGACACA 
GATGTAGAGG AGAGCGATAC TTCAATCCCT GGAATAAATG TACCCCGAGT GGAGAATTCT 
GGGAATAACA CAGACAGTGA TACAGATACT GAAGAAGATA AACCTAAAAT TTTGCAACCT 
GCCATACAAC AGAATGTCGA GGAAGGGTTT AATTTGGACA GTGACACGGA TGTTGAGGGT 
AATGACTCCA ATTCTGTAAA TAAAAGGTTG AAAAATACTG AAAAAGCAAA AATGATCCAA 
GATAGTGATA CTGATGCGGA TGATTCGATT ATGTCAAAGG TTGAAGAGAG GAGGAACCCA 
GCAGGTACAA AGAAAGATTA CAGTAGTACA TCTCTAAACA CAGAAGGTAA AAGGCAAGAA 
AATTATCTAT CCACCCTTGA CAGTGATACA GATGTTGAAG ACATTGCTGA TGGATCTTCC 
CCAAGTGTTG TGAAGAAGCA GGATAAGGCA GAAGCAGCTC ATACTAATGT AACCGATACA 
ACTGCTGGGG AGGAAACTTG CACAGATGCT GGAATAGAGA AGATTGTGGT GTCTGCAGAT 
AGAAATGAAT GCCACACAGG TGGAGTCACA CAAGAACAGG AGGCTACATC TGAATTTCAC 
CTAGACAGTG ATACAGATAT TGAGGAAGAT GATAATTTAC TAGATATGTC AAGTGCTAGT 
ACTAGGGTAA AAGTCACAGA GGTTCACTCA GTTATCCTTG AAAAACTAAG AACCTCACCT 
GGAGAAAGAA AGGAAAAGAG GGCTATTGAA ATTGATCTGG ACAGCAATAC AGATGTGGAT 
GAAGCTGATG AAACAGAAGT TGATAGGAAG GAAACATTTC ACATGGACAG TGATACAGAT 
CTGGAGGAAA ATGACAGTGG CCCTCCTGTG GTGGGTGGCA TAGAAAAGAA ATTTACAGCC 
ATAGCACATT CTGATTGTAC CTCAGACACA GGAAGTAATG TGAAAAATGT AACCTCTTCA 
ATCACAGAAA ATACAGAAAA AGGGGAGACT GATGGAATAG ACTGCGAAGA TGCAGAATAT 
CAAATGATGG CTACTCAGTG TTATCTAGAG CCCCAGGAAA AAGAGACTGA TATACAAGAT 
GAAGATGATT GTGCTGAGGA AGCTACACAA GCATTTATCT TCAGTTCCAC ATGGGCTGAG 
CCGGAACCAT TTAAACGTCC AGCCGATCCT ATTGGAGTTT TGCAAATCTC AGCAGTGACT 
GTTAGTTCAT CAGAGGAAGA ACCAGATGAA AATGCCATTG CGGAGACTCA GCCATTCTGC 
TGTGAGATAA AGCAGCCAGA GGAACACAGT GACCAAGAGC CAGCCAAAAA AAGTTCTATT 
GCTGAGGAAA AGAACAATGT AGCTTGCTCT CTGCCTCAAG AAGAAATATC TCAGGATGAT 
ACCCAGCCAG TGTCTCAGTA CTTGAATAAA ACAGCTTCAA GTAACTTGGT GGCACCAAAT 
TTAACTTGCA CAACAAATTT TCAACAAGAA AAATATCTTG ATGGAATTAA TGAAGAAAAG 
GTCAAACAGG CTAATGAAAG TGTTCCAAAC AGGGAGGAAC AGCTTGATGA AGGTGTCCAA 
GACATCGAAG AGGATGCCAC CCAACCATAT GTTTTAAGTT TACCGACAGT GGGTGACATG 
GCTACACAGC CTTGTAGCAT GAGTGAGTCT GTCTCCGGGG ATGGTGCTAG TCAGTATTTA 
AATACAGTTG TTCCAGCTGT AGCTAGTGTT GCTTCTCTAC CCTGTAACCC AAGCATGCAA 
TTATCTGATG GCGAACCCAT GCAGCCTTCC AGTCTAAATG AGGCTGATGC AACCCAATGT 
CATGGATTTA GTATGCCCCT TAAAGAAGGT GATACAGTGA GCATTTCAGA AGAGAAAGTA 
TCAGTAAAGC AAGGTCCTTG CAGAGGTGAG AGTAGCATGA TATCTGAGCA GTTAGACCCT 
TCTAACAATG TTATGAGCAA GCACCCAGGT GAAGTTCTTG TGCATTCTGT TCCTCCATTG 
GAGAAAGCAA CAGAGCAGGT TGCAGAGCAG CCTCAAGCAG CCACCAGAAT GACAGAAGAA 
GACAGAGAAG AGAATAAAGG AAAGAGGAGA TCAACAGAAG GAGAAGATAG CAAGGATTCA 
GCAGAAGTTA CAACTTCCGT TACTAGGGGT AAAAAGAGAA CCTGGAAAAA GACTGCATCT 
CCAGCATCAG AGGAGACAAA AGGCAAAAAA ACAGCTAAAA TGCAGGTCAT GGAAAAAGCA 
TTACCTGAAG CAAAAATGGA AGAAGAATGT AAAGAAGAGC AGAACAATAA GCCAAACACT 
AGCTCTAGCC TTGCACGTTT AAAAACAGGT AGAAAGGGAA AACAAGATAT CGAAGAGCCT 
TTAGATTACA ATGTAGAAGG CTATAATGTG AGCGTGCAAC AGGTAGAAGA GGATATGAAA 
AGATTTACTT CAAGAAGATC AGTTAGAAAA CCAAAGGATG GAAATGATCA GGAGACTGGG 
TCAAGAAATT TACCAGAAAT AACCAGGCAT TCCAGTTTAG ACCTTAGTGA GTCAGTAGTG 
GAAAAAAAAG TAGATATGTC TTCTGATGAA CACATGAAGT ATCAGAAAGA TGATGAATTC 
TTAACTGCTA ATGGAACAAC TATAAATGTA AAAAGAAAGG GTGCAAAGAG AAATGTAAAG 
GAAGAGGAGA ACAAGTTTGT GGTAGATAAC ATTCCTGGAA AGCGACCAAC AAGGAATAAC 
AGGCCAAATG TTACAGAGAA AAATAATGAG GAAGCAGATG ATAAACAGAG GATTACAAGG 
AGGAATTCAA GGCAGCTGGA GATAGTAGAA ACCAAACCAG ACAATACAGA GATTTTAGAT 
TCTAAAAGAC AAAACCAGTC CAGAAAATCA CCAAATATAA AGAAAAATTT AAAAGTGGAT 
GAATCAAAAG AGACAGAAAA ATGTGAGGGC AAAACTAGAG AGAACACTCC GAAAAGGACA 
TCAAGGAGGA CAAAGAAAAA ATGCACAGAG GAACTGAACA TGGAAGAATG GGAAAAGTCA 
AAAGAAGAAG CAGACAGTAA ACAGAGGGTC ACAAGAAGGA ACTCCAGGCT ATCAGAGTCA 
GTAGAAAGCA TACCAGAGGA TACAGATATT GTAGATTCTG AGCAACAAGA CCAGTCCAGA 
AAATCTCGTA ATATAAAGAA GAATTTGAAA AGGGGCGAGG AAGAAAAACT TGAGGGCACC 
AACAGAGACA ACACTGAAAA ATGGACCTCA AGGAGGACAA GAAAGGAATG CAAAGAGAAA 
CTGAACATGG AGGACCATGA AAAGTCGAAA GTAGAACCAT CCATTGAACA GTCTGAACAA 
GAACATACTG AGGCTGAAGG ACAAGATCAA TCTAGAAAAT CTAGGAAGAC AAAGAAGGAT 
TTGCCACAAC CTGTTATCAA TGAGGAAGAA ACCCTTGTAG AAACCATCAG GAAAGACTCT 
GTAGGACGAA CATCAAGGAG AAGAAAGAAG AGTTGCATAG AGGAAGATCT GATGGACAGT 
GAGGTTCTAG TTATCAGTAA ACCTGAACAA GAAGATCCAC CCACAAAAAA TGTTCCTAGA 
AGTAAAAGGA AGAATTTGAA AGATGAACCT AAGGAGGGAG ATACTGAGCA TAATGTATTT 
GTATCAAAGA ATGAAAATCT AGATGAAATG CAGACCACTC AAAGGAAAAG CAATTCAAAA 
GATGGAGATG TGCAAAACGA TAATAAAACA CTGTGTGAAG TCGACTCCAA GTCTGGTAAT 
TCAGATATAA GAACAAGGAA GAAAAAAAAA GTTGTCACAG GGACTCAAAA AACCACAGAG 
GTAGAAGGGG AAATGCTAGA TGGTAGCATG ACAATAGACT CGCACGAAGA CGTTTCACCA 
ACTGGAATGA GAAAAAGCAG GCGGACAACA ACCAAGGAGG AATTACTCAA GTCTACCACT 
CTGGCTCCAG TAAGGAAGAG AGGGCAACAG CCAAAAACTG GAGGAGATGA GGTCAAGAGA 
AAAAAAGTGA ATGAAGAGTT TGAGCAGGAG AAAACAGTGG GTAGAAGGGG CCAAGCACGG 
CAACCAAAGC TGGAGGATGA AAATACTGAC GGCAACAGTT TTGGAAAGGA CTCGGCCGTT 
CCTTTTCCAT CACCCTCGGG GAACTCCCGT TTAAGGCCAA GCACATCCTT TGAAAGCCCG 
ACAGAAGGCC GGACACCAAG ACGTGCTGCT CGCAGCTTGA CCACAAGCCC CTACATGTCC 
CAACGGAGCA TTGCAGCAAA GGTTCTATTT ACAGGTGTGG TCGACCCTGC AGGTGAGGAG 
ACCATTCGTA ATCTAGGAGG GGAAGTTGCA GAATCCATAT TTGACTGCAC TCATCTTGTA 
ACTGACCGTA TCAGACGTAC CGTCAAATTT CTCTGTGCTC TTGCCAAGGG CATCCCTATT 
GTGACACTAG ACTGGCTTGA CAAGTGCAAA AAGAGCAGAT GTTTTTTGTC CCCTGCTCAA 
TTTCTGGTGA ACGATAAAGA ACAGGAGAAA AGCTTCAACT TCGATTTGTC AGAATCATTG 
CAAAAAGCCA AAAAGAAACT GCTGTTTGAG GGCTATGAAA TTCATGTCAC CCCGAATGTG 
AAACCTGAGC CTGAACACAT GAAAGATATT ATACAGTGCA GTGGGGCAAC TTTCCTTCCT 
AAAATGCCCA AAGTATACAA GGACAAATGC ATCATTGTAT CATGTAAGGA AGATGCCACT 
CGCTGCAAAT CTGCTCCCTC CACTATCCCT GTTACTTCTG CGGAATTTAT ATTAACTGGG 
ATCCTGCGTC AGGAAATAAA TCCACATGCT TATCTGCTAA GTACTGGACC TGAGGAAGCT 
TTGCCTACCC CTGCCAAACG GAGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824676.1
CDS	883..6372
Translation	MDTIVPESDEENDTSVSEPRFPSLNLRCDSDTDTHDTPMKAGTSYTPSQDCVLSPSFKEQVVGDHSEITKSTLISNQEMFADEQVNTESKVSKCSPDTADKNDRETKEENNTPIVNTNIAEEGGTGVTDDPLIDGLKVENQKKENDCYETLQVDIKQQESVKIILDSNQEENRTLKVKLVNTGSADIILDKNIDGEEGCVNTKEIDNQIREGAFHLDSDTDVEESDTSIPGINVPRVENSGNNTDSDTDTEEDKPKILQPAIQQNVEEGFNLDSDTDVEGNDSNSVNKRLKNTEKAKMIQDSDTDADDSIMSKVEERRNPAGTKKDYSSTSLNTEGKRQENYLSTLDSDTDVEDIADGSSPSVVKKQDKAEAAHTNVTDTTAGEETCTDAGIEKIVVSADRNECHTGGVTQEQEATSEFHLDSDTDIEEDDNLLDMSSASTRVKVTEVHSVILEKLRTSPGERKEKRAIEIDLDSNTDVDEADETEVDRKETFHMDSDTDLEENDSGPPVVGGIEKKFTAIAHSDCTSDTGSNVKNVTSSITENTEKGETDGIDCEDAEYQMMATQCYLEPQEKETDIQDEDDCAEEATQAFIFSSTWAEPEPFKRPADPIGVLQISAVTVSSSEEEPDENAIAETQPFCCEIKQPEEHSDQEPAKKSSIAEEKNNVACSLPQEEISQDDTQPVSQYLNKTASSNLVAPNLTCTTNFQQEKYLDGINEEKVKQANESVPNREEQLDEGVQDIEEDATQPYVLSLPTVGDMATQPCSMSESVSGDGASQYLNTVVPAVASVASLPCNPSMQLSDGEPMQPSSLNEADATQCHGFSMPLKEGDTVSISEEKVSVKQGPCRGESSMISEQLDPSNNVMSKHPGEVLVHSVPPLEKATEQVAEQPQAATRMTEEDREENKGKRRSTEGEDSKDSAEVTTSVTRGKKRTWKKTASPASEETKGKKTAKMQVMEKALPEAKMEEECKEEQNNKPNTSSSLARLKTGRKGKQDIEEPLDYNVEGYNVSVQQVEEDMKRFTSRRSVRKPKDGNDQETGSRNLPEITRHSSLDLSESVVEKKVDMSSDEHMKYQKDDEFLTANGTTINVKRKGAKRNVKEEENKFVVDNIPGKRPTRNNRPNVTEKNNEEADDKQRITRRNSRQLEIVETKPDNTEILDSKRQNQSRKSPNIKKNLKVDESKETEKCEGKTRENTPKRTSRRTKKKCTEELNMEEWEKSKEEADSKQRVTRRNSRLSESVESIPEDTDIVDSEQQDQSRKSRNIKKNLKRGEEEKLEGTNRDNTEKWTSRRTRKECKEKLNMEDHEKSKVEPSIEQSEQEHTEAEGQDQSRKSRKTKKDLPQPVINEEETLVETIRKDSVGRTSRRRKKSCIEEDLMDSEVLVISKPEQEDPPTKNVPRSKRKNLKDEPKEGDTEHNVFVSKNENLDEMQTTQRKSNSKDGDVQNDNKTLCEVDSKSGNSDIRTRKKKKVVTGTQKTTEVEGEMLDGSMTIDSHEDVSPTGMRKSRRTTTKEELLKSTTLAPVRKRGQQPKTGGDEVKRKKVNEEFEQEKTVGRRGQARQPKLEDENTDGNSFGKDSAVPFPSPSGNSRLRPSTSFESPTEGRTPRRAARSLTTSPYMSQRSIAAKVLFTGVVDPAGEETIRNLGGEVAESIFDCTHLVTDRIRRTVKFLCALAKGIPIVTLDWLDKCKKSRCFLSPAQFLVNDKEQEKSFNFDLSESLQKAKKKLLFEGYEIHVTPNVKPEPEHMKDIIQCSGATFLPKMPKVYKDKCIIVSCKEDATRCKSAPSTIPVTSAEFILTGILRQEINPHAYLLSTGPEEALPTPAKRRR

Target ORF information:

RefSeq Version	XM_012969222.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969222.2

ORF Insert Sequence:

ATGGATACTA TTGTTCCTGA AAGTGATGAG GAGAACGACA CATCTGTGTC TGAACCACGT 
TTCCCTTCAC TCAACCTCCG TTGTGATAGT GATACAGACA CTCATGACAC TCCCATGAAG 
GCAGGCACAA GCTACACCCC CTCCCAAGAT TGTGTGCTTT CTCCTTCTTT CAAAGAACAA 
GTTGTGGGAG ACCACAGTGA AATCACAAAA TCTACTTTGA TTAGCAACCA AGAGATGTTT 
GCAGATGAGC AAGTAAACAC TGAATCCAAA GTCTCGAAGT GTAGTCCTGA TACAGCTGAC 
AAAAATGATA GAGAGACAAA GGAAGAGAAC AATACTCCTA TAGTAAACAC TAATATTGCT 
GAGGAAGGTG GAACAGGAGT AACAGATGAT CCATTGATAG ACGGTCTAAA AGTGGAAAAC 
CAAAAAAAGG AAAATGACTG TTATGAAACC CTGCAAGTTG ACATCAAACA GCAAGAGAGT 
GTTAAAATTA TCTTGGATAG TAATCAGGAG GAAAACAGAA CTTTGAAAGT AAAATTGGTA 
AACACAGGAT CTGCAGATAT TATTCTAGAC AAAAATATAG ATGGGGAAGA AGGTTGCGTC 
AATACTAAGG AAATAGACAA CCAGATCAGA GAAGGGGCTT TTCATTTGGA CAGTGACACA 
GATGTAGAGG AGAGCGATAC TTCAATCCCT GGAATAAATG TACCCCGAGT GGAGAATTCT 
GGGAATAACA CAGACAGTGA TACAGATACT GAAGAAGATA AACCTAAAAT TTTGCAACCT 
GCCATACAAC AGAATGTCGA GGAAGGGTTT AATTTGGACA GTGACACGGA TGTTGAGGGT 
AATGACTCCA ATTCTGTAAA TAAAAGGTTG AAAAATACTG AAAAAGCAAA AATGATCCAA 
GATAGTGATA CTGATGCGGA TGATTCGATT ATGTCAAAGG TTGAAGAGAG GAGGAACCCA 
GCAGGTACAA AGAAAGATTA CAGTAGTACA TCTCTAAACA CAGAAGGTAA AAGGCAAGAA 
AATTATCTAT CCACCCTTGA CAGTGATACA GATGTTGAAG ACATTGCTGA TGGATCTTCC 
CCAAGTGTTG TGAAGAAGCA GGATAAGGCA GAAGCAGCTC ATACTAATGT AACCGATACA 
ACTGCTGGGG AGGAAACTTG CACAGATGCT GGAATAGAGA AGATTGTGGT GTCTGCAGAT 
AGAAATGAAT GCCACACAGG TGGAGTCACA CAAGAACAGG AGGCTACATC TGAATTTCAC 
CTAGACAGTG ATACAGATAT TGAGGAAGAT GATAATTTAC TAGATATGTC AAGTGCTAGT 
ACTAGGGTAA AAGTCACAGA GGTTCACTCA GTTATCCTTG AAAAACTAAG AACCTCACCT 
GGAGAAAGAA AGGAAAAGAG GGCTATTGAA ATTGATCTGG ACAGCAATAC AGATGTGGAT 
GAAGCTGATG AAACAGAAGT TGATAGGAAG GAAACATTTC ACATGGACAG TGATACAGAT 
CTGGAGGAAA ATGACAGTGG CCCTCCTGTG GTGGGTGGCA TAGAAAAGAA ATTTACAGCC 
ATAGCACATT CTGATTGTAC CTCAGACACA GGAAGTAATG TGAAAAATGT AACCTCTTCA 
ATCACAGAAA ATACAGAAAA AGGGGAGACT GATGGAATAG ACTGCGAAGA TGCAGAATAT 
CAAATGATGG CTACTCAGTG TTATCTAGAG CCCCAGGAAA AAGAGACTGA TATACAAGAT 
GAAGATGATT GTGCTGAGGA AGCTACACAA GCATTTATCT TCAGTTCCAC ATGGGCTGAG 
CCGGAACCAT TTAAACGTCC AGCCGATCCT ATTGGAGTTT TGCAAATCTC AGCAGTGACT 
GTTAGTTCAT CAGAGGAAGA ACCAGATGAA AATGCCATTG CGGAGACTCA GCCATTCTGC 
TGTGAGATAA AGCAGCCAGA GGAACACAGT GACCAAGAGC CAGCCAAAAA AAGTTCTATT 
GCTGAGGAAA AGAACAATGT AGCTTGCTCT CTGCCTCAAG AAGAAATATC TCAGGATGAT 
ACCCAGCCAG TGTCTCAGTA CTTGAATAAA ACAGCTTCAA GTAACTTGGT GGCACCAAAT 
TTAACTTGCA CAACAAATTT TCAACAAGAA AAATATCTTG ATGGAATTAA TGAAGAAAAG 
GTCAAACAGG CTAATGAAAG TGTTCCAAAC AGGGAGGAAC AGCTTGATGA AGGTGTCCAA 
GACATCGAAG AGGATGCCAC CCAACCATAT GTTTTAAGTT TACCGACAGT GGGTGACATG 
GCTACACAGC CTTGTAGCAT GAGTGAGTCT GTCTCCGGGG ATGGTGCTAG TCAGTATTTA 
AATACAGTTG TTCCAGCTGT AGCTAGTGTT GCTTCTCTAC CCTGTAACCC AAGCATGCAA 
TTATCTGATG GCGAACCCAT GCAGCCTTCC AGTCTAAATG AGGCTGATGC AACCCAATGT 
CATGGATTTA GTATGCCCCT TAAAGAAGGT GATACAGTGA GCATTTCAGA AGAGAAAGTA 
TCAGTAAAGC AAGGTCCTTG CAGAGGTGAG AGTAGCATGA TATCTGAGCA GTTAGACCCT 
TCTAACAATG TTATGAGCAA GCACCCAGGT GAAGTTCTTG TGCATTCTGT TCCTCCATTG 
GAGAAAGCAA CAGAGCAGGT TGCAGAGCAG CCTCAAGCAG CCACCAGAAT GACAGAAGAA 
GACAGAGAAG AGAATAAAGG AAAGAGGAGA TCAACAGAAG GAGAAGATAG CAAGGATTCA 
GCAGAAGTTA CAACTTCCGT TACTAGGGGT AAAAAGAGAA CCTGGAAAAA GACTGCATCT 
CCAGCATCAG AGGAGACAAA AGGCAAAAAA ACAGCTAAAA TGCAGGTCAT GGAAAAAGCA 
TTACCTGAAG CAAAAATGGA AGAAGAATGT AAAGAAGAGC AGAACAATAA GCCAAACACT 
AGCTCTAGCC TTGCACGTTT AAAAACAGGT AGAAAGGGAA AACAAGATAT CGAAGAGCCT 
TTAGATTACA ATGTAGAAGG CTATAATGTG AGCGTGCAAC AGGTAGAAGA GGATATGAAA 
AGATTTACTT CAAGAAGATC AGTTAGAAAA CCAAAGGATG GAAATGATCA GGAGACTGGG 
TCAAGAAATT TACCAGAAAT AACCAGGCAT TCCAGTTTAG ACCTTAGTGA GTCAGTAGTG 
GAAAAAAAAG TAGATATGTC TTCTGATGAA CACATGAAGT ATCAGAAAGA TGATGAATTC 
TTAACTGCTA ATGGAACAAC TATAAATGTA AAAAGAAAGG GTGCAAAGAG AAATGTAAAG 
GAAGAGGAGA ACAAGTTTGT GGTAGATAAC ATTCCTGGAA AGCGACCAAC AAGGAATAAC 
AGGCCAAATG TTACAGAGAA AAATAATGAG GAAGCAGATG ATAAACAGAG GATTACAAGG 
AGGAATTCAA GGCAGCTGGA GATAGTAGAA ACCAAACCAG ACAATACAGA GATTTTAGAT 
TCTAAAAGAC AAAACCAGTC CAGAAAATCA CCAAATATAA AGAAAAATTT AAAAGTGGAT 
GAATCAAAAG AGACAGAAAA ATGTGAGGGC AAAACTAGAG AGAACACTCC GAAAAGGACA 
TCAAGGAGGA CAAAGAAAAA ATGCACAGAG GAACTGAACA TGGAAGAATG GGAAAAGTCA 
AAAGAAGAAG CAGACAGTAA ACAGAGGGTC ACAAGAAGGA ACTCCAGGCT ATCAGAGTCA 
GTAGAAAGCA TACCAGAGGA TACAGATATT GTAGATTCTG AGCAACAAGA CCAGTCCAGA 
AAATCTCGTA ATATAAAGAA GAATTTGAAA AGGGGCGAGG AAGAAAAACT TGAGGGCACC 
AACAGAGACA ACACTGAAAA ATGGACCTCA AGGAGGACAA GAAAGGAATG CAAAGAGAAA 
CTGAACATGG AGGACCATGA AAAGTCGAAA GTAGAACCAT CCATTGAACA GTCTGAACAA 
GAACATACTG AGGCTGAAGG ACAAGATCAA TCTAGAAAAT CTAGGAAGAC AAAGAAGGAT 
TTGCCACAAC CTGTTATCAA TGAGGAAGAA ACCCTTGTAG AAACCATCAG GAAAGACTCT 
GTAGGACGAA CATCAAGGAG AAGAAAGAAG AGTTGCATAG AGGAAGATCT GATGGACAGT 
GAGGTTCTAG TTATCAGTAA ACCTGAACAA GAAGATCCAC CCACAAAAAA TGTTCCTAGA 
AGTAAAAGGA AGAATTTGAA AGATGAACCT AAGGAGGGAG ATACTGAGCA TAATGTATTT 
GTATCAAAGA ATGAAAATCT AGATGAAATG CAGACCACTC AAAGGAAAAG CAATTCAAAA 
GATGGAGATG TGCAAAACGA TAATAAAACA CTGTGTGAAG TCGACTCCAA GTCTGGTAAT 
TCAGATATAA GAACAAGGAA GAAAAAAAAA GTTGTCACAG GGACTCAAAA AACCACAGAG 
GTAGAAGGGG AAATGCTAGA TGGTAGCATG ACAATAGACT CGCACGAAGA CGTTTCACCA 
ACTGGAATGA GAAAAAGCAG GCGGACAACA ACCAAGGAGG AATTACTCAA GTCTACCACT 
CTGGCTCCAG TAAGGAAGAG AGGGCAACAG CCAAAAACTG GAGGAGATGA GGTCAAGAGA 
AAAAAAGTGA ATGAAGAGTT TGAGCAGGAG AAAACAGTGG GTAGAAGGGG CCAAGCACGG 
CAACCAAAGC TGGAGGATGA AAATACTGAC GGCAACAGTT TTGGAAAGGA CTCGGCCGTT 
CCTTTTCCAT CACCCTCGGG GAACTCCCGT TTAAGGCCAA GCACATCCTT TGAAAGCCCG 
ACAGAAGGCC GGACACCAAG ACGTGCTGCT CGCAGCTTGA CCACAAGCCC CTACATGTCC 
CAACGGAGCA TTGCAGCAAA GGTTCTATTT ACAGGTGTGG TCGACCCTGC AGGTGAGGAG 
ACCATTCGTA ATCTAGGAGG GGAAGTTGCA GAATCCATAT TTGACTGCAC TCATCTTGTA 
ACTGACCGTA TCAGACGTAC CGTCAAATTT CTCTGTGCTC TTGCCAAGGG CATCCCTATT 
GTGACACTAG ACTGGCTTGA CAAGTGCAAA AAGAGCAGAT GTTTTTTGTC CCCTGCTCAA 
TTTCTGGTGA ACGATAAAGA ACAGGAGAAA AGCTTCAACT TCGATTTGTC AGAATCATTG 
CAAAAAGCCA AAAAGAAACT GCTGTTTGAG GGCTATGAAA TTCATGTCAC CCCGAATGTG 
AAACCTGAGC CTGAACACAT GAAAGATATT ATACAGTGCA GTGGGGCAAC TTTCCTTCCT 
AAAATGCCCA AAGTATACAA GGACAAATGC ATCATTGTAT CATGTAAGGA AGATGCCACT 
CGCTGCAAAT CTGCTCCCTC CACTATCCCT GTTACTTCTG CGGAATTTAT ATTAACTGGG 
ATCCTGCGTC AGGAAATAAA TCCACATGCT TATCTGCTAA GTACTGGACC TGAGGAAGCT 
TTGCCTACCC CTGCCAAACG GAGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdc1 ( XM_002939467.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939513.2 Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.

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CloneID	OXa14440
Clone ID Related Accession (Same CDS sequence)	XM_012969222.2 , XM_002939467.4 , XM_004916906.3
Accession Version	XM_002939467.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939467.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA TTGTTCCTGA AAGTGATGAG GAGAACGACA CATCTGTGTC TGAACCACGT 
TTCCCTTCAC TCAACCTCCG TTGTGATAGT GATACAGACA CTCATGACAC TCCCATGAAG 
GCAGGCACAA GCTACACCCC CTCCCAAGAT TGTGTGCTTT CTCCTTCTTT CAAAGAACAA 
GTTGTGGGAG ACCACAGTGA AATCACAAAA TCTACTTTGA TTAGCAACCA AGAGATGTTT 
GCAGATGAGC AAGTAAACAC TGAATCCAAA GTCTCGAAGT GTAGTCCTGA TACAGCTGAC 
AAAAATGATA GAGAGACAAA GGAAGAGAAC AATACTCCTA TAGTAAACAC TAATATTGCT 
GAGGAAGGTG GAACAGGAGT AACAGATGAT CCATTGATAG ACGGTCTAAA AGTGGAAAAC 
CAAAAAAAGG AAAATGACTG TTATGAAACC CTGCAAGTTG ACATCAAACA GCAAGAGAGT 
GTTAAAATTA TCTTGGATAG TAATCAGGAG GAAAACAGAA CTTTGAAAGT AAAATTGGTA 
AACACAGGAT CTGCAGATAT TATTCTAGAC AAAAATATAG ATGGGGAAGA AGGTTGCGTC 
AATACTAAGG AAATAGACAA CCAGATCAGA GAAGGGGCTT TTCATTTGGA CAGTGACACA 
GATGTAGAGG AGAGCGATAC TTCAATCCCT GGAATAAATG TACCCCGAGT GGAGAATTCT 
GGGAATAACA CAGACAGTGA TACAGATACT GAAGAAGATA AACCTAAAAT TTTGCAACCT 
GCCATACAAC AGAATGTCGA GGAAGGGTTT AATTTGGACA GTGACACGGA TGTTGAGGGT 
AATGACTCCA ATTCTGTAAA TAAAAGGTTG AAAAATACTG AAAAAGCAAA AATGATCCAA 
GATAGTGATA CTGATGCGGA TGATTCGATT ATGTCAAAGG TTGAAGAGAG GAGGAACCCA 
GCAGGTACAA AGAAAGATTA CAGTAGTACA TCTCTAAACA CAGAAGGTAA AAGGCAAGAA 
AATTATCTAT CCACCCTTGA CAGTGATACA GATGTTGAAG ACATTGCTGA TGGATCTTCC 
CCAAGTGTTG TGAAGAAGCA GGATAAGGCA GAAGCAGCTC ATACTAATGT AACCGATACA 
ACTGCTGGGG AGGAAACTTG CACAGATGCT GGAATAGAGA AGATTGTGGT GTCTGCAGAT 
AGAAATGAAT GCCACACAGG TGGAGTCACA CAAGAACAGG AGGCTACATC TGAATTTCAC 
CTAGACAGTG ATACAGATAT TGAGGAAGAT GATAATTTAC TAGATATGTC AAGTGCTAGT 
ACTAGGGTAA AAGTCACAGA GGTTCACTCA GTTATCCTTG AAAAACTAAG AACCTCACCT 
GGAGAAAGAA AGGAAAAGAG GGCTATTGAA ATTGATCTGG ACAGCAATAC AGATGTGGAT 
GAAGCTGATG AAACAGAAGT TGATAGGAAG GAAACATTTC ACATGGACAG TGATACAGAT 
CTGGAGGAAA ATGACAGTGG CCCTCCTGTG GTGGGTGGCA TAGAAAAGAA ATTTACAGCC 
ATAGCACATT CTGATTGTAC CTCAGACACA GGAAGTAATG TGAAAAATGT AACCTCTTCA 
ATCACAGAAA ATACAGAAAA AGGGGAGACT GATGGAATAG ACTGCGAAGA TGCAGAATAT 
CAAATGATGG CTACTCAGTG TTATCTAGAG CCCCAGGAAA AAGAGACTGA TATACAAGAT 
GAAGATGATT GTGCTGAGGA AGCTACACAA GCATTTATCT TCAGTTCCAC ATGGGCTGAG 
CCGGAACCAT TTAAACGTCC AGCCGATCCT ATTGGAGTTT TGCAAATCTC AGCAGTGACT 
GTTAGTTCAT CAGAGGAAGA ACCAGATGAA AATGCCATTG CGGAGACTCA GCCATTCTGC 
TGTGAGATAA AGCAGCCAGA GGAACACAGT GACCAAGAGC CAGCCAAAAA AAGTTCTATT 
GCTGAGGAAA AGAACAATGT AGCTTGCTCT CTGCCTCAAG AAGAAATATC TCAGGATGAT 
ACCCAGCCAG TGTCTCAGTA CTTGAATAAA ACAGCTTCAA GTAACTTGGT GGCACCAAAT 
TTAACTTGCA CAACAAATTT TCAACAAGAA AAATATCTTG ATGGAATTAA TGAAGAAAAG 
GTCAAACAGG CTAATGAAAG TGTTCCAAAC AGGGAGGAAC AGCTTGATGA AGGTGTCCAA 
GACATCGAAG AGGATGCCAC CCAACCATAT GTTTTAAGTT TACCGACAGT GGGTGACATG 
GCTACACAGC CTTGTAGCAT GAGTGAGTCT GTCTCCGGGG ATGGTGCTAG TCAGTATTTA 
AATACAGTTG TTCCAGCTGT AGCTAGTGTT GCTTCTCTAC CCTGTAACCC AAGCATGCAA 
TTATCTGATG GCGAACCCAT GCAGCCTTCC AGTCTAAATG AGGCTGATGC AACCCAATGT 
CATGGATTTA GTATGCCCCT TAAAGAAGGT GATACAGTGA GCATTTCAGA AGAGAAAGTA 
TCAGTAAAGC AAGGTCCTTG CAGAGGTGAG AGTAGCATGA TATCTGAGCA GTTAGACCCT 
TCTAACAATG TTATGAGCAA GCACCCAGGT GAAGTTCTTG TGCATTCTGT TCCTCCATTG 
GAGAAAGCAA CAGAGCAGGT TGCAGAGCAG CCTCAAGCAG CCACCAGAAT GACAGAAGAA 
GACAGAGAAG AGAATAAAGG AAAGAGGAGA TCAACAGAAG GAGAAGATAG CAAGGATTCA 
GCAGAAGTTA CAACTTCCGT TACTAGGGGT AAAAAGAGAA CCTGGAAAAA GACTGCATCT 
CCAGCATCAG AGGAGACAAA AGGCAAAAAA ACAGCTAAAA TGCAGGTCAT GGAAAAAGCA 
TTACCTGAAG CAAAAATGGA AGAAGAATGT AAAGAAGAGC AGAACAATAA GCCAAACACT 
AGCTCTAGCC TTGCACGTTT AAAAACAGGT AGAAAGGGAA AACAAGATAT CGAAGAGCCT 
TTAGATTACA ATGTAGAAGG CTATAATGTG AGCGTGCAAC AGGTAGAAGA GGATATGAAA 
AGATTTACTT CAAGAAGATC AGTTAGAAAA CCAAAGGATG GAAATGATCA GGAGACTGGG 
TCAAGAAATT TACCAGAAAT AACCAGGCAT TCCAGTTTAG ACCTTAGTGA GTCAGTAGTG 
GAAAAAAAAG TAGATATGTC TTCTGATGAA CACATGAAGT ATCAGAAAGA TGATGAATTC 
TTAACTGCTA ATGGAACAAC TATAAATGTA AAAAGAAAGG GTGCAAAGAG AAATGTAAAG 
GAAGAGGAGA ACAAGTTTGT GGTAGATAAC ATTCCTGGAA AGCGACCAAC AAGGAATAAC 
AGGCCAAATG TTACAGAGAA AAATAATGAG GAAGCAGATG ATAAACAGAG GATTACAAGG 
AGGAATTCAA GGCAGCTGGA GATAGTAGAA ACCAAACCAG ACAATACAGA GATTTTAGAT 
TCTAAAAGAC AAAACCAGTC CAGAAAATCA CCAAATATAA AGAAAAATTT AAAAGTGGAT 
GAATCAAAAG AGACAGAAAA ATGTGAGGGC AAAACTAGAG AGAACACTCC GAAAAGGACA 
TCAAGGAGGA CAAAGAAAAA ATGCACAGAG GAACTGAACA TGGAAGAATG GGAAAAGTCA 
AAAGAAGAAG CAGACAGTAA ACAGAGGGTC ACAAGAAGGA ACTCCAGGCT ATCAGAGTCA 
GTAGAAAGCA TACCAGAGGA TACAGATATT GTAGATTCTG AGCAACAAGA CCAGTCCAGA 
AAATCTCGTA ATATAAAGAA GAATTTGAAA AGGGGCGAGG AAGAAAAACT TGAGGGCACC 
AACAGAGACA ACACTGAAAA ATGGACCTCA AGGAGGACAA GAAAGGAATG CAAAGAGAAA 
CTGAACATGG AGGACCATGA AAAGTCGAAA GTAGAACCAT CCATTGAACA GTCTGAACAA 
GAACATACTG AGGCTGAAGG ACAAGATCAA TCTAGAAAAT CTAGGAAGAC AAAGAAGGAT 
TTGCCACAAC CTGTTATCAA TGAGGAAGAA ACCCTTGTAG AAACCATCAG GAAAGACTCT 
GTAGGACGAA CATCAAGGAG AAGAAAGAAG AGTTGCATAG AGGAAGATCT GATGGACAGT 
GAGGTTCTAG TTATCAGTAA ACCTGAACAA GAAGATCCAC CCACAAAAAA TGTTCCTAGA 
AGTAAAAGGA AGAATTTGAA AGATGAACCT AAGGAGGGAG ATACTGAGCA TAATGTATTT 
GTATCAAAGA ATGAAAATCT AGATGAAATG CAGACCACTC AAAGGAAAAG CAATTCAAAA 
GATGGAGATG TGCAAAACGA TAATAAAACA CTGTGTGAAG TCGACTCCAA GTCTGGTAAT 
TCAGATATAA GAACAAGGAA GAAAAAAAAA GTTGTCACAG GGACTCAAAA AACCACAGAG 
GTAGAAGGGG AAATGCTAGA TGGTAGCATG ACAATAGACT CGCACGAAGA CGTTTCACCA 
ACTGGAATGA GAAAAAGCAG GCGGACAACA ACCAAGGAGG AATTACTCAA GTCTACCACT 
CTGGCTCCAG TAAGGAAGAG AGGGCAACAG CCAAAAACTG GAGGAGATGA GGTCAAGAGA 
AAAAAAGTGA ATGAAGAGTT TGAGCAGGAG AAAACAGTGG GTAGAAGGGG CCAAGCACGG 
CAACCAAAGC TGGAGGATGA AAATACTGAC GGCAACAGTT TTGGAAAGGA CTCGGCCGTT 
CCTTTTCCAT CACCCTCGGG GAACTCCCGT TTAAGGCCAA GCACATCCTT TGAAAGCCCG 
ACAGAAGGCC GGACACCAAG ACGTGCTGCT CGCAGCTTGA CCACAAGCCC CTACATGTCC 
CAACGGAGCA TTGCAGCAAA GGTTCTATTT ACAGGTGTGG TCGACCCTGC AGGTGAGGAG 
ACCATTCGTA ATCTAGGAGG GGAAGTTGCA GAATCCATAT TTGACTGCAC TCATCTTGTA 
ACTGACCGTA TCAGACGTAC CGTCAAATTT CTCTGTGCTC TTGCCAAGGG CATCCCTATT 
GTGACACTAG ACTGGCTTGA CAAGTGCAAA AAGAGCAGAT GTTTTTTGTC CCCTGCTCAA 
TTTCTGGTGA ACGATAAAGA ACAGGAGAAA AGCTTCAACT TCGATTTGTC AGAATCATTG 
CAAAAAGCCA AAAAGAAACT GCTGTTTGAG GGCTATGAAA TTCATGTCAC CCCGAATGTG 
AAACCTGAGC CTGAACACAT GAAAGATATT ATACAGTGCA GTGGGGCAAC TTTCCTTCCT 
AAAATGCCCA AAGTATACAA GGACAAATGC ATCATTGTAT CATGTAAGGA AGATGCCACT 
CGCTGCAAAT CTGCTCCCTC CACTATCCCT GTTACTTCTG CGGAATTTAT ATTAACTGGG 
ATCCTGCGTC AGGAAATAAA TCCACATGCT TATCTGCTAA GTACTGGACC TGAGGAAGCT 
TTGCCTACCC CTGCCAAACG GAGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939513.2
CDS	856..6345
Translation	MDTIVPESDEENDTSVSEPRFPSLNLRCDSDTDTHDTPMKAGTSYTPSQDCVLSPSFKEQVVGDHSEITKSTLISNQEMFADEQVNTESKVSKCSPDTADKNDRETKEENNTPIVNTNIAEEGGTGVTDDPLIDGLKVENQKKENDCYETLQVDIKQQESVKIILDSNQEENRTLKVKLVNTGSADIILDKNIDGEEGCVNTKEIDNQIREGAFHLDSDTDVEESDTSIPGINVPRVENSGNNTDSDTDTEEDKPKILQPAIQQNVEEGFNLDSDTDVEGNDSNSVNKRLKNTEKAKMIQDSDTDADDSIMSKVEERRNPAGTKKDYSSTSLNTEGKRQENYLSTLDSDTDVEDIADGSSPSVVKKQDKAEAAHTNVTDTTAGEETCTDAGIEKIVVSADRNECHTGGVTQEQEATSEFHLDSDTDIEEDDNLLDMSSASTRVKVTEVHSVILEKLRTSPGERKEKRAIEIDLDSNTDVDEADETEVDRKETFHMDSDTDLEENDSGPPVVGGIEKKFTAIAHSDCTSDTGSNVKNVTSSITENTEKGETDGIDCEDAEYQMMATQCYLEPQEKETDIQDEDDCAEEATQAFIFSSTWAEPEPFKRPADPIGVLQISAVTVSSSEEEPDENAIAETQPFCCEIKQPEEHSDQEPAKKSSIAEEKNNVACSLPQEEISQDDTQPVSQYLNKTASSNLVAPNLTCTTNFQQEKYLDGINEEKVKQANESVPNREEQLDEGVQDIEEDATQPYVLSLPTVGDMATQPCSMSESVSGDGASQYLNTVVPAVASVASLPCNPSMQLSDGEPMQPSSLNEADATQCHGFSMPLKEGDTVSISEEKVSVKQGPCRGESSMISEQLDPSNNVMSKHPGEVLVHSVPPLEKATEQVAEQPQAATRMTEEDREENKGKRRSTEGEDSKDSAEVTTSVTRGKKRTWKKTASPASEETKGKKTAKMQVMEKALPEAKMEEECKEEQNNKPNTSSSLARLKTGRKGKQDIEEPLDYNVEGYNVSVQQVEEDMKRFTSRRSVRKPKDGNDQETGSRNLPEITRHSSLDLSESVVEKKVDMSSDEHMKYQKDDEFLTANGTTINVKRKGAKRNVKEEENKFVVDNIPGKRPTRNNRPNVTEKNNEEADDKQRITRRNSRQLEIVETKPDNTEILDSKRQNQSRKSPNIKKNLKVDESKETEKCEGKTRENTPKRTSRRTKKKCTEELNMEEWEKSKEEADSKQRVTRRNSRLSESVESIPEDTDIVDSEQQDQSRKSRNIKKNLKRGEEEKLEGTNRDNTEKWTSRRTRKECKEKLNMEDHEKSKVEPSIEQSEQEHTEAEGQDQSRKSRKTKKDLPQPVINEEETLVETIRKDSVGRTSRRRKKSCIEEDLMDSEVLVISKPEQEDPPTKNVPRSKRKNLKDEPKEGDTEHNVFVSKNENLDEMQTTQRKSNSKDGDVQNDNKTLCEVDSKSGNSDIRTRKKKKVVTGTQKTTEVEGEMLDGSMTIDSHEDVSPTGMRKSRRTTTKEELLKSTTLAPVRKRGQQPKTGGDEVKRKKVNEEFEQEKTVGRRGQARQPKLEDENTDGNSFGKDSAVPFPSPSGNSRLRPSTSFESPTEGRTPRRAARSLTTSPYMSQRSIAAKVLFTGVVDPAGEETIRNLGGEVAESIFDCTHLVTDRIRRTVKFLCALAKGIPIVTLDWLDKCKKSRCFLSPAQFLVNDKEQEKSFNFDLSESLQKAKKKLLFEGYEIHVTPNVKPEPEHMKDIIQCSGATFLPKMPKVYKDKCIIVSCKEDATRCKSAPSTIPVTSAEFILTGILRQEINPHAYLLSTGPEEALPTPAKRRR

Target ORF information:

RefSeq Version	XM_002939467.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939467.4

ORF Insert Sequence:

ATGGATACTA TTGTTCCTGA AAGTGATGAG GAGAACGACA CATCTGTGTC TGAACCACGT 
TTCCCTTCAC TCAACCTCCG TTGTGATAGT GATACAGACA CTCATGACAC TCCCATGAAG 
GCAGGCACAA GCTACACCCC CTCCCAAGAT TGTGTGCTTT CTCCTTCTTT CAAAGAACAA 
GTTGTGGGAG ACCACAGTGA AATCACAAAA TCTACTTTGA TTAGCAACCA AGAGATGTTT 
GCAGATGAGC AAGTAAACAC TGAATCCAAA GTCTCGAAGT GTAGTCCTGA TACAGCTGAC 
AAAAATGATA GAGAGACAAA GGAAGAGAAC AATACTCCTA TAGTAAACAC TAATATTGCT 
GAGGAAGGTG GAACAGGAGT AACAGATGAT CCATTGATAG ACGGTCTAAA AGTGGAAAAC 
CAAAAAAAGG AAAATGACTG TTATGAAACC CTGCAAGTTG ACATCAAACA GCAAGAGAGT 
GTTAAAATTA TCTTGGATAG TAATCAGGAG GAAAACAGAA CTTTGAAAGT AAAATTGGTA 
AACACAGGAT CTGCAGATAT TATTCTAGAC AAAAATATAG ATGGGGAAGA AGGTTGCGTC 
AATACTAAGG AAATAGACAA CCAGATCAGA GAAGGGGCTT TTCATTTGGA CAGTGACACA 
GATGTAGAGG AGAGCGATAC TTCAATCCCT GGAATAAATG TACCCCGAGT GGAGAATTCT 
GGGAATAACA CAGACAGTGA TACAGATACT GAAGAAGATA AACCTAAAAT TTTGCAACCT 
GCCATACAAC AGAATGTCGA GGAAGGGTTT AATTTGGACA GTGACACGGA TGTTGAGGGT 
AATGACTCCA ATTCTGTAAA TAAAAGGTTG AAAAATACTG AAAAAGCAAA AATGATCCAA 
GATAGTGATA CTGATGCGGA TGATTCGATT ATGTCAAAGG TTGAAGAGAG GAGGAACCCA 
GCAGGTACAA AGAAAGATTA CAGTAGTACA TCTCTAAACA CAGAAGGTAA AAGGCAAGAA 
AATTATCTAT CCACCCTTGA CAGTGATACA GATGTTGAAG ACATTGCTGA TGGATCTTCC 
CCAAGTGTTG TGAAGAAGCA GGATAAGGCA GAAGCAGCTC ATACTAATGT AACCGATACA 
ACTGCTGGGG AGGAAACTTG CACAGATGCT GGAATAGAGA AGATTGTGGT GTCTGCAGAT 
AGAAATGAAT GCCACACAGG TGGAGTCACA CAAGAACAGG AGGCTACATC TGAATTTCAC 
CTAGACAGTG ATACAGATAT TGAGGAAGAT GATAATTTAC TAGATATGTC AAGTGCTAGT 
ACTAGGGTAA AAGTCACAGA GGTTCACTCA GTTATCCTTG AAAAACTAAG AACCTCACCT 
GGAGAAAGAA AGGAAAAGAG GGCTATTGAA ATTGATCTGG ACAGCAATAC AGATGTGGAT 
GAAGCTGATG AAACAGAAGT TGATAGGAAG GAAACATTTC ACATGGACAG TGATACAGAT 
CTGGAGGAAA ATGACAGTGG CCCTCCTGTG GTGGGTGGCA TAGAAAAGAA ATTTACAGCC 
ATAGCACATT CTGATTGTAC CTCAGACACA GGAAGTAATG TGAAAAATGT AACCTCTTCA 
ATCACAGAAA ATACAGAAAA AGGGGAGACT GATGGAATAG ACTGCGAAGA TGCAGAATAT 
CAAATGATGG CTACTCAGTG TTATCTAGAG CCCCAGGAAA AAGAGACTGA TATACAAGAT 
GAAGATGATT GTGCTGAGGA AGCTACACAA GCATTTATCT TCAGTTCCAC ATGGGCTGAG 
CCGGAACCAT TTAAACGTCC AGCCGATCCT ATTGGAGTTT TGCAAATCTC AGCAGTGACT 
GTTAGTTCAT CAGAGGAAGA ACCAGATGAA AATGCCATTG CGGAGACTCA GCCATTCTGC 
TGTGAGATAA AGCAGCCAGA GGAACACAGT GACCAAGAGC CAGCCAAAAA AAGTTCTATT 
GCTGAGGAAA AGAACAATGT AGCTTGCTCT CTGCCTCAAG AAGAAATATC TCAGGATGAT 
ACCCAGCCAG TGTCTCAGTA CTTGAATAAA ACAGCTTCAA GTAACTTGGT GGCACCAAAT 
TTAACTTGCA CAACAAATTT TCAACAAGAA AAATATCTTG ATGGAATTAA TGAAGAAAAG 
GTCAAACAGG CTAATGAAAG TGTTCCAAAC AGGGAGGAAC AGCTTGATGA AGGTGTCCAA 
GACATCGAAG AGGATGCCAC CCAACCATAT GTTTTAAGTT TACCGACAGT GGGTGACATG 
GCTACACAGC CTTGTAGCAT GAGTGAGTCT GTCTCCGGGG ATGGTGCTAG TCAGTATTTA 
AATACAGTTG TTCCAGCTGT AGCTAGTGTT GCTTCTCTAC CCTGTAACCC AAGCATGCAA 
TTATCTGATG GCGAACCCAT GCAGCCTTCC AGTCTAAATG AGGCTGATGC AACCCAATGT 
CATGGATTTA GTATGCCCCT TAAAGAAGGT GATACAGTGA GCATTTCAGA AGAGAAAGTA 
TCAGTAAAGC AAGGTCCTTG CAGAGGTGAG AGTAGCATGA TATCTGAGCA GTTAGACCCT 
TCTAACAATG TTATGAGCAA GCACCCAGGT GAAGTTCTTG TGCATTCTGT TCCTCCATTG 
GAGAAAGCAA CAGAGCAGGT TGCAGAGCAG CCTCAAGCAG CCACCAGAAT GACAGAAGAA 
GACAGAGAAG AGAATAAAGG AAAGAGGAGA TCAACAGAAG GAGAAGATAG CAAGGATTCA 
GCAGAAGTTA CAACTTCCGT TACTAGGGGT AAAAAGAGAA CCTGGAAAAA GACTGCATCT 
CCAGCATCAG AGGAGACAAA AGGCAAAAAA ACAGCTAAAA TGCAGGTCAT GGAAAAAGCA 
TTACCTGAAG CAAAAATGGA AGAAGAATGT AAAGAAGAGC AGAACAATAA GCCAAACACT 
AGCTCTAGCC TTGCACGTTT AAAAACAGGT AGAAAGGGAA AACAAGATAT CGAAGAGCCT 
TTAGATTACA ATGTAGAAGG CTATAATGTG AGCGTGCAAC AGGTAGAAGA GGATATGAAA 
AGATTTACTT CAAGAAGATC AGTTAGAAAA CCAAAGGATG GAAATGATCA GGAGACTGGG 
TCAAGAAATT TACCAGAAAT AACCAGGCAT TCCAGTTTAG ACCTTAGTGA GTCAGTAGTG 
GAAAAAAAAG TAGATATGTC TTCTGATGAA CACATGAAGT ATCAGAAAGA TGATGAATTC 
TTAACTGCTA ATGGAACAAC TATAAATGTA AAAAGAAAGG GTGCAAAGAG AAATGTAAAG 
GAAGAGGAGA ACAAGTTTGT GGTAGATAAC ATTCCTGGAA AGCGACCAAC AAGGAATAAC 
AGGCCAAATG TTACAGAGAA AAATAATGAG GAAGCAGATG ATAAACAGAG GATTACAAGG 
AGGAATTCAA GGCAGCTGGA GATAGTAGAA ACCAAACCAG ACAATACAGA GATTTTAGAT 
TCTAAAAGAC AAAACCAGTC CAGAAAATCA CCAAATATAA AGAAAAATTT AAAAGTGGAT 
GAATCAAAAG AGACAGAAAA ATGTGAGGGC AAAACTAGAG AGAACACTCC GAAAAGGACA 
TCAAGGAGGA CAAAGAAAAA ATGCACAGAG GAACTGAACA TGGAAGAATG GGAAAAGTCA 
AAAGAAGAAG CAGACAGTAA ACAGAGGGTC ACAAGAAGGA ACTCCAGGCT ATCAGAGTCA 
GTAGAAAGCA TACCAGAGGA TACAGATATT GTAGATTCTG AGCAACAAGA CCAGTCCAGA 
AAATCTCGTA ATATAAAGAA GAATTTGAAA AGGGGCGAGG AAGAAAAACT TGAGGGCACC 
AACAGAGACA ACACTGAAAA ATGGACCTCA AGGAGGACAA GAAAGGAATG CAAAGAGAAA 
CTGAACATGG AGGACCATGA AAAGTCGAAA GTAGAACCAT CCATTGAACA GTCTGAACAA 
GAACATACTG AGGCTGAAGG ACAAGATCAA TCTAGAAAAT CTAGGAAGAC AAAGAAGGAT 
TTGCCACAAC CTGTTATCAA TGAGGAAGAA ACCCTTGTAG AAACCATCAG GAAAGACTCT 
GTAGGACGAA CATCAAGGAG AAGAAAGAAG AGTTGCATAG AGGAAGATCT GATGGACAGT 
GAGGTTCTAG TTATCAGTAA ACCTGAACAA GAAGATCCAC CCACAAAAAA TGTTCCTAGA 
AGTAAAAGGA AGAATTTGAA AGATGAACCT AAGGAGGGAG ATACTGAGCA TAATGTATTT 
GTATCAAAGA ATGAAAATCT AGATGAAATG CAGACCACTC AAAGGAAAAG CAATTCAAAA 
GATGGAGATG TGCAAAACGA TAATAAAACA CTGTGTGAAG TCGACTCCAA GTCTGGTAAT 
TCAGATATAA GAACAAGGAA GAAAAAAAAA GTTGTCACAG GGACTCAAAA AACCACAGAG 
GTAGAAGGGG AAATGCTAGA TGGTAGCATG ACAATAGACT CGCACGAAGA CGTTTCACCA 
ACTGGAATGA GAAAAAGCAG GCGGACAACA ACCAAGGAGG AATTACTCAA GTCTACCACT 
CTGGCTCCAG TAAGGAAGAG AGGGCAACAG CCAAAAACTG GAGGAGATGA GGTCAAGAGA 
AAAAAAGTGA ATGAAGAGTT TGAGCAGGAG AAAACAGTGG GTAGAAGGGG CCAAGCACGG 
CAACCAAAGC TGGAGGATGA AAATACTGAC GGCAACAGTT TTGGAAAGGA CTCGGCCGTT 
CCTTTTCCAT CACCCTCGGG GAACTCCCGT TTAAGGCCAA GCACATCCTT TGAAAGCCCG 
ACAGAAGGCC GGACACCAAG ACGTGCTGCT CGCAGCTTGA CCACAAGCCC CTACATGTCC 
CAACGGAGCA TTGCAGCAAA GGTTCTATTT ACAGGTGTGG TCGACCCTGC AGGTGAGGAG 
ACCATTCGTA ATCTAGGAGG GGAAGTTGCA GAATCCATAT TTGACTGCAC TCATCTTGTA 
ACTGACCGTA TCAGACGTAC CGTCAAATTT CTCTGTGCTC TTGCCAAGGG CATCCCTATT 
GTGACACTAG ACTGGCTTGA CAAGTGCAAA AAGAGCAGAT GTTTTTTGTC CCCTGCTCAA 
TTTCTGGTGA ACGATAAAGA ACAGGAGAAA AGCTTCAACT TCGATTTGTC AGAATCATTG 
CAAAAAGCCA AAAAGAAACT GCTGTTTGAG GGCTATGAAA TTCATGTCAC CCCGAATGTG 
AAACCTGAGC CTGAACACAT GAAAGATATT ATACAGTGCA GTGGGGCAAC TTTCCTTCCT 
AAAATGCCCA AAGTATACAA GGACAAATGC ATCATTGTAT CATGTAAGGA AGATGCCACT 
CGCTGCAAAT CTGCTCCCTC CACTATCCCT GTTACTTCTG CGGAATTTAT ATTAACTGGG 
ATCCTGCGTC AGGAAATAAA TCCACATGCT TATCTGCTAA GTACTGGACC TGAGGAAGCT 
TTGCCTACCC CTGCCAAACG GAGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdc1 ( XM_004916906.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916963.1 Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.

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CloneID	OXa14440
Clone ID Related Accession (Same CDS sequence)	XM_012969222.2 , XM_002939467.4 , XM_004916906.3
Accession Version	XM_004916906.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA TTGTTCCTGA AAGTGATGAG GAGAACGACA CATCTGTGTC TGAACCACGT 
TTCCCTTCAC TCAACCTCCG TTGTGATAGT GATACAGACA CTCATGACAC TCCCATGAAG 
GCAGGCACAA GCTACACCCC CTCCCAAGAT TGTGTGCTTT CTCCTTCTTT CAAAGAACAA 
GTTGTGGGAG ACCACAGTGA AATCACAAAA TCTACTTTGA TTAGCAACCA AGAGATGTTT 
GCAGATGAGC AAGTAAACAC TGAATCCAAA GTCTCGAAGT GTAGTCCTGA TACAGCTGAC 
AAAAATGATA GAGAGACAAA GGAAGAGAAC AATACTCCTA TAGTAAACAC TAATATTGCT 
GAGGAAGGTG GAACAGGAGT AACAGATGAT CCATTGATAG ACGGTCTAAA AGTGGAAAAC 
CAAAAAAAGG AAAATGACTG TTATGAAACC CTGCAAGTTG ACATCAAACA GCAAGAGAGT 
GTTAAAATTA TCTTGGATAG TAATCAGGAG GAAAACAGAA CTTTGAAAGT AAAATTGGTA 
AACACAGGAT CTGCAGATAT TATTCTAGAC AAAAATATAG ATGGGGAAGA AGGTTGCGTC 
AATACTAAGG AAATAGACAA CCAGATCAGA GAAGGGGCTT TTCATTTGGA CAGTGACACA 
GATGTAGAGG AGAGCGATAC TTCAATCCCT GGAATAAATG TACCCCGAGT GGAGAATTCT 
GGGAATAACA CAGACAGTGA TACAGATACT GAAGAAGATA AACCTAAAAT TTTGCAACCT 
GCCATACAAC AGAATGTCGA GGAAGGGTTT AATTTGGACA GTGACACGGA TGTTGAGGGT 
AATGACTCCA ATTCTGTAAA TAAAAGGTTG AAAAATACTG AAAAAGCAAA AATGATCCAA 
GATAGTGATA CTGATGCGGA TGATTCGATT ATGTCAAAGG TTGAAGAGAG GAGGAACCCA 
GCAGGTACAA AGAAAGATTA CAGTAGTACA TCTCTAAACA CAGAAGGTAA AAGGCAAGAA 
AATTATCTAT CCACCCTTGA CAGTGATACA GATGTTGAAG ACATTGCTGA TGGATCTTCC 
CCAAGTGTTG TGAAGAAGCA GGATAAGGCA GAAGCAGCTC ATACTAATGT AACCGATACA 
ACTGCTGGGG AGGAAACTTG CACAGATGCT GGAATAGAGA AGATTGTGGT GTCTGCAGAT 
AGAAATGAAT GCCACACAGG TGGAGTCACA CAAGAACAGG AGGCTACATC TGAATTTCAC 
CTAGACAGTG ATACAGATAT TGAGGAAGAT GATAATTTAC TAGATATGTC AAGTGCTAGT 
ACTAGGGTAA AAGTCACAGA GGTTCACTCA GTTATCCTTG AAAAACTAAG AACCTCACCT 
GGAGAAAGAA AGGAAAAGAG GGCTATTGAA ATTGATCTGG ACAGCAATAC AGATGTGGAT 
GAAGCTGATG AAACAGAAGT TGATAGGAAG GAAACATTTC ACATGGACAG TGATACAGAT 
CTGGAGGAAA ATGACAGTGG CCCTCCTGTG GTGGGTGGCA TAGAAAAGAA ATTTACAGCC 
ATAGCACATT CTGATTGTAC CTCAGACACA GGAAGTAATG TGAAAAATGT AACCTCTTCA 
ATCACAGAAA ATACAGAAAA AGGGGAGACT GATGGAATAG ACTGCGAAGA TGCAGAATAT 
CAAATGATGG CTACTCAGTG TTATCTAGAG CCCCAGGAAA AAGAGACTGA TATACAAGAT 
GAAGATGATT GTGCTGAGGA AGCTACACAA GCATTTATCT TCAGTTCCAC ATGGGCTGAG 
CCGGAACCAT TTAAACGTCC AGCCGATCCT ATTGGAGTTT TGCAAATCTC AGCAGTGACT 
GTTAGTTCAT CAGAGGAAGA ACCAGATGAA AATGCCATTG CGGAGACTCA GCCATTCTGC 
TGTGAGATAA AGCAGCCAGA GGAACACAGT GACCAAGAGC CAGCCAAAAA AAGTTCTATT 
GCTGAGGAAA AGAACAATGT AGCTTGCTCT CTGCCTCAAG AAGAAATATC TCAGGATGAT 
ACCCAGCCAG TGTCTCAGTA CTTGAATAAA ACAGCTTCAA GTAACTTGGT GGCACCAAAT 
TTAACTTGCA CAACAAATTT TCAACAAGAA AAATATCTTG ATGGAATTAA TGAAGAAAAG 
GTCAAACAGG CTAATGAAAG TGTTCCAAAC AGGGAGGAAC AGCTTGATGA AGGTGTCCAA 
GACATCGAAG AGGATGCCAC CCAACCATAT GTTTTAAGTT TACCGACAGT GGGTGACATG 
GCTACACAGC CTTGTAGCAT GAGTGAGTCT GTCTCCGGGG ATGGTGCTAG TCAGTATTTA 
AATACAGTTG TTCCAGCTGT AGCTAGTGTT GCTTCTCTAC CCTGTAACCC AAGCATGCAA 
TTATCTGATG GCGAACCCAT GCAGCCTTCC AGTCTAAATG AGGCTGATGC AACCCAATGT 
CATGGATTTA GTATGCCCCT TAAAGAAGGT GATACAGTGA GCATTTCAGA AGAGAAAGTA 
TCAGTAAAGC AAGGTCCTTG CAGAGGTGAG AGTAGCATGA TATCTGAGCA GTTAGACCCT 
TCTAACAATG TTATGAGCAA GCACCCAGGT GAAGTTCTTG TGCATTCTGT TCCTCCATTG 
GAGAAAGCAA CAGAGCAGGT TGCAGAGCAG CCTCAAGCAG CCACCAGAAT GACAGAAGAA 
GACAGAGAAG AGAATAAAGG AAAGAGGAGA TCAACAGAAG GAGAAGATAG CAAGGATTCA 
GCAGAAGTTA CAACTTCCGT TACTAGGGGT AAAAAGAGAA CCTGGAAAAA GACTGCATCT 
CCAGCATCAG AGGAGACAAA AGGCAAAAAA ACAGCTAAAA TGCAGGTCAT GGAAAAAGCA 
TTACCTGAAG CAAAAATGGA AGAAGAATGT AAAGAAGAGC AGAACAATAA GCCAAACACT 
AGCTCTAGCC TTGCACGTTT AAAAACAGGT AGAAAGGGAA AACAAGATAT CGAAGAGCCT 
TTAGATTACA ATGTAGAAGG CTATAATGTG AGCGTGCAAC AGGTAGAAGA GGATATGAAA 
AGATTTACTT CAAGAAGATC AGTTAGAAAA CCAAAGGATG GAAATGATCA GGAGACTGGG 
TCAAGAAATT TACCAGAAAT AACCAGGCAT TCCAGTTTAG ACCTTAGTGA GTCAGTAGTG 
GAAAAAAAAG TAGATATGTC TTCTGATGAA CACATGAAGT ATCAGAAAGA TGATGAATTC 
TTAACTGCTA ATGGAACAAC TATAAATGTA AAAAGAAAGG GTGCAAAGAG AAATGTAAAG 
GAAGAGGAGA ACAAGTTTGT GGTAGATAAC ATTCCTGGAA AGCGACCAAC AAGGAATAAC 
AGGCCAAATG TTACAGAGAA AAATAATGAG GAAGCAGATG ATAAACAGAG GATTACAAGG 
AGGAATTCAA GGCAGCTGGA GATAGTAGAA ACCAAACCAG ACAATACAGA GATTTTAGAT 
TCTAAAAGAC AAAACCAGTC CAGAAAATCA CCAAATATAA AGAAAAATTT AAAAGTGGAT 
GAATCAAAAG AGACAGAAAA ATGTGAGGGC AAAACTAGAG AGAACACTCC GAAAAGGACA 
TCAAGGAGGA CAAAGAAAAA ATGCACAGAG GAACTGAACA TGGAAGAATG GGAAAAGTCA 
AAAGAAGAAG CAGACAGTAA ACAGAGGGTC ACAAGAAGGA ACTCCAGGCT ATCAGAGTCA 
GTAGAAAGCA TACCAGAGGA TACAGATATT GTAGATTCTG AGCAACAAGA CCAGTCCAGA 
AAATCTCGTA ATATAAAGAA GAATTTGAAA AGGGGCGAGG AAGAAAAACT TGAGGGCACC 
AACAGAGACA ACACTGAAAA ATGGACCTCA AGGAGGACAA GAAAGGAATG CAAAGAGAAA 
CTGAACATGG AGGACCATGA AAAGTCGAAA GTAGAACCAT CCATTGAACA GTCTGAACAA 
GAACATACTG AGGCTGAAGG ACAAGATCAA TCTAGAAAAT CTAGGAAGAC AAAGAAGGAT 
TTGCCACAAC CTGTTATCAA TGAGGAAGAA ACCCTTGTAG AAACCATCAG GAAAGACTCT 
GTAGGACGAA CATCAAGGAG AAGAAAGAAG AGTTGCATAG AGGAAGATCT GATGGACAGT 
GAGGTTCTAG TTATCAGTAA ACCTGAACAA GAAGATCCAC CCACAAAAAA TGTTCCTAGA 
AGTAAAAGGA AGAATTTGAA AGATGAACCT AAGGAGGGAG ATACTGAGCA TAATGTATTT 
GTATCAAAGA ATGAAAATCT AGATGAAATG CAGACCACTC AAAGGAAAAG CAATTCAAAA 
GATGGAGATG TGCAAAACGA TAATAAAACA CTGTGTGAAG TCGACTCCAA GTCTGGTAAT 
TCAGATATAA GAACAAGGAA GAAAAAAAAA GTTGTCACAG GGACTCAAAA AACCACAGAG 
GTAGAAGGGG AAATGCTAGA TGGTAGCATG ACAATAGACT CGCACGAAGA CGTTTCACCA 
ACTGGAATGA GAAAAAGCAG GCGGACAACA ACCAAGGAGG AATTACTCAA GTCTACCACT 
CTGGCTCCAG TAAGGAAGAG AGGGCAACAG CCAAAAACTG GAGGAGATGA GGTCAAGAGA 
AAAAAAGTGA ATGAAGAGTT TGAGCAGGAG AAAACAGTGG GTAGAAGGGG CCAAGCACGG 
CAACCAAAGC TGGAGGATGA AAATACTGAC GGCAACAGTT TTGGAAAGGA CTCGGCCGTT 
CCTTTTCCAT CACCCTCGGG GAACTCCCGT TTAAGGCCAA GCACATCCTT TGAAAGCCCG 
ACAGAAGGCC GGACACCAAG ACGTGCTGCT CGCAGCTTGA CCACAAGCCC CTACATGTCC 
CAACGGAGCA TTGCAGCAAA GGTTCTATTT ACAGGTGTGG TCGACCCTGC AGGTGAGGAG 
ACCATTCGTA ATCTAGGAGG GGAAGTTGCA GAATCCATAT TTGACTGCAC TCATCTTGTA 
ACTGACCGTA TCAGACGTAC CGTCAAATTT CTCTGTGCTC TTGCCAAGGG CATCCCTATT 
GTGACACTAG ACTGGCTTGA CAAGTGCAAA AAGAGCAGAT GTTTTTTGTC CCCTGCTCAA 
TTTCTGGTGA ACGATAAAGA ACAGGAGAAA AGCTTCAACT TCGATTTGTC AGAATCATTG 
CAAAAAGCCA AAAAGAAACT GCTGTTTGAG GGCTATGAAA TTCATGTCAC CCCGAATGTG 
AAACCTGAGC CTGAACACAT GAAAGATATT ATACAGTGCA GTGGGGCAAC TTTCCTTCCT 
AAAATGCCCA AAGTATACAA GGACAAATGC ATCATTGTAT CATGTAAGGA AGATGCCACT 
CGCTGCAAAT CTGCTCCCTC CACTATCCCT GTTACTTCTG CGGAATTTAT ATTAACTGGG 
ATCCTGCGTC AGGAAATAAA TCCACATGCT TATCTGCTAA GTACTGGACC TGAGGAAGCT 
TTGCCTACCC CTGCCAAACG GAGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916963.1
CDS	707..6196
Translation	MDTIVPESDEENDTSVSEPRFPSLNLRCDSDTDTHDTPMKAGTSYTPSQDCVLSPSFKEQVVGDHSEITKSTLISNQEMFADEQVNTESKVSKCSPDTADKNDRETKEENNTPIVNTNIAEEGGTGVTDDPLIDGLKVENQKKENDCYETLQVDIKQQESVKIILDSNQEENRTLKVKLVNTGSADIILDKNIDGEEGCVNTKEIDNQIREGAFHLDSDTDVEESDTSIPGINVPRVENSGNNTDSDTDTEEDKPKILQPAIQQNVEEGFNLDSDTDVEGNDSNSVNKRLKNTEKAKMIQDSDTDADDSIMSKVEERRNPAGTKKDYSSTSLNTEGKRQENYLSTLDSDTDVEDIADGSSPSVVKKQDKAEAAHTNVTDTTAGEETCTDAGIEKIVVSADRNECHTGGVTQEQEATSEFHLDSDTDIEEDDNLLDMSSASTRVKVTEVHSVILEKLRTSPGERKEKRAIEIDLDSNTDVDEADETEVDRKETFHMDSDTDLEENDSGPPVVGGIEKKFTAIAHSDCTSDTGSNVKNVTSSITENTEKGETDGIDCEDAEYQMMATQCYLEPQEKETDIQDEDDCAEEATQAFIFSSTWAEPEPFKRPADPIGVLQISAVTVSSSEEEPDENAIAETQPFCCEIKQPEEHSDQEPAKKSSIAEEKNNVACSLPQEEISQDDTQPVSQYLNKTASSNLVAPNLTCTTNFQQEKYLDGINEEKVKQANESVPNREEQLDEGVQDIEEDATQPYVLSLPTVGDMATQPCSMSESVSGDGASQYLNTVVPAVASVASLPCNPSMQLSDGEPMQPSSLNEADATQCHGFSMPLKEGDTVSISEEKVSVKQGPCRGESSMISEQLDPSNNVMSKHPGEVLVHSVPPLEKATEQVAEQPQAATRMTEEDREENKGKRRSTEGEDSKDSAEVTTSVTRGKKRTWKKTASPASEETKGKKTAKMQVMEKALPEAKMEEECKEEQNNKPNTSSSLARLKTGRKGKQDIEEPLDYNVEGYNVSVQQVEEDMKRFTSRRSVRKPKDGNDQETGSRNLPEITRHSSLDLSESVVEKKVDMSSDEHMKYQKDDEFLTANGTTINVKRKGAKRNVKEEENKFVVDNIPGKRPTRNNRPNVTEKNNEEADDKQRITRRNSRQLEIVETKPDNTEILDSKRQNQSRKSPNIKKNLKVDESKETEKCEGKTRENTPKRTSRRTKKKCTEELNMEEWEKSKEEADSKQRVTRRNSRLSESVESIPEDTDIVDSEQQDQSRKSRNIKKNLKRGEEEKLEGTNRDNTEKWTSRRTRKECKEKLNMEDHEKSKVEPSIEQSEQEHTEAEGQDQSRKSRKTKKDLPQPVINEEETLVETIRKDSVGRTSRRRKKSCIEEDLMDSEVLVISKPEQEDPPTKNVPRSKRKNLKDEPKEGDTEHNVFVSKNENLDEMQTTQRKSNSKDGDVQNDNKTLCEVDSKSGNSDIRTRKKKKVVTGTQKTTEVEGEMLDGSMTIDSHEDVSPTGMRKSRRTTTKEELLKSTTLAPVRKRGQQPKTGGDEVKRKKVNEEFEQEKTVGRRGQARQPKLEDENTDGNSFGKDSAVPFPSPSGNSRLRPSTSFESPTEGRTPRRAARSLTTSPYMSQRSIAAKVLFTGVVDPAGEETIRNLGGEVAESIFDCTHLVTDRIRRTVKFLCALAKGIPIVTLDWLDKCKKSRCFLSPAQFLVNDKEQEKSFNFDLSESLQKAKKKLLFEGYEIHVTPNVKPEPEHMKDIIQCSGATFLPKMPKVYKDKCIIVSCKEDATRCKSAPSTIPVTSAEFILTGILRQEINPHAYLLSTGPEEALPTPAKRRR

Target ORF information:

RefSeq Version	XM_004916906.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916906.3

ORF Insert Sequence:

ATGGATACTA TTGTTCCTGA AAGTGATGAG GAGAACGACA CATCTGTGTC TGAACCACGT 
TTCCCTTCAC TCAACCTCCG TTGTGATAGT GATACAGACA CTCATGACAC TCCCATGAAG 
GCAGGCACAA GCTACACCCC CTCCCAAGAT TGTGTGCTTT CTCCTTCTTT CAAAGAACAA 
GTTGTGGGAG ACCACAGTGA AATCACAAAA TCTACTTTGA TTAGCAACCA AGAGATGTTT 
GCAGATGAGC AAGTAAACAC TGAATCCAAA GTCTCGAAGT GTAGTCCTGA TACAGCTGAC 
AAAAATGATA GAGAGACAAA GGAAGAGAAC AATACTCCTA TAGTAAACAC TAATATTGCT 
GAGGAAGGTG GAACAGGAGT AACAGATGAT CCATTGATAG ACGGTCTAAA AGTGGAAAAC 
CAAAAAAAGG AAAATGACTG TTATGAAACC CTGCAAGTTG ACATCAAACA GCAAGAGAGT 
GTTAAAATTA TCTTGGATAG TAATCAGGAG GAAAACAGAA CTTTGAAAGT AAAATTGGTA 
AACACAGGAT CTGCAGATAT TATTCTAGAC AAAAATATAG ATGGGGAAGA AGGTTGCGTC 
AATACTAAGG AAATAGACAA CCAGATCAGA GAAGGGGCTT TTCATTTGGA CAGTGACACA 
GATGTAGAGG AGAGCGATAC TTCAATCCCT GGAATAAATG TACCCCGAGT GGAGAATTCT 
GGGAATAACA CAGACAGTGA TACAGATACT GAAGAAGATA AACCTAAAAT TTTGCAACCT 
GCCATACAAC AGAATGTCGA GGAAGGGTTT AATTTGGACA GTGACACGGA TGTTGAGGGT 
AATGACTCCA ATTCTGTAAA TAAAAGGTTG AAAAATACTG AAAAAGCAAA AATGATCCAA 
GATAGTGATA CTGATGCGGA TGATTCGATT ATGTCAAAGG TTGAAGAGAG GAGGAACCCA 
GCAGGTACAA AGAAAGATTA CAGTAGTACA TCTCTAAACA CAGAAGGTAA AAGGCAAGAA 
AATTATCTAT CCACCCTTGA CAGTGATACA GATGTTGAAG ACATTGCTGA TGGATCTTCC 
CCAAGTGTTG TGAAGAAGCA GGATAAGGCA GAAGCAGCTC ATACTAATGT AACCGATACA 
ACTGCTGGGG AGGAAACTTG CACAGATGCT GGAATAGAGA AGATTGTGGT GTCTGCAGAT 
AGAAATGAAT GCCACACAGG TGGAGTCACA CAAGAACAGG AGGCTACATC TGAATTTCAC 
CTAGACAGTG ATACAGATAT TGAGGAAGAT GATAATTTAC TAGATATGTC AAGTGCTAGT 
ACTAGGGTAA AAGTCACAGA GGTTCACTCA GTTATCCTTG AAAAACTAAG AACCTCACCT 
GGAGAAAGAA AGGAAAAGAG GGCTATTGAA ATTGATCTGG ACAGCAATAC AGATGTGGAT 
GAAGCTGATG AAACAGAAGT TGATAGGAAG GAAACATTTC ACATGGACAG TGATACAGAT 
CTGGAGGAAA ATGACAGTGG CCCTCCTGTG GTGGGTGGCA TAGAAAAGAA ATTTACAGCC 
ATAGCACATT CTGATTGTAC CTCAGACACA GGAAGTAATG TGAAAAATGT AACCTCTTCA 
ATCACAGAAA ATACAGAAAA AGGGGAGACT GATGGAATAG ACTGCGAAGA TGCAGAATAT 
CAAATGATGG CTACTCAGTG TTATCTAGAG CCCCAGGAAA AAGAGACTGA TATACAAGAT 
GAAGATGATT GTGCTGAGGA AGCTACACAA GCATTTATCT TCAGTTCCAC ATGGGCTGAG 
CCGGAACCAT TTAAACGTCC AGCCGATCCT ATTGGAGTTT TGCAAATCTC AGCAGTGACT 
GTTAGTTCAT CAGAGGAAGA ACCAGATGAA AATGCCATTG CGGAGACTCA GCCATTCTGC 
TGTGAGATAA AGCAGCCAGA GGAACACAGT GACCAAGAGC CAGCCAAAAA AAGTTCTATT 
GCTGAGGAAA AGAACAATGT AGCTTGCTCT CTGCCTCAAG AAGAAATATC TCAGGATGAT 
ACCCAGCCAG TGTCTCAGTA CTTGAATAAA ACAGCTTCAA GTAACTTGGT GGCACCAAAT 
TTAACTTGCA CAACAAATTT TCAACAAGAA AAATATCTTG ATGGAATTAA TGAAGAAAAG 
GTCAAACAGG CTAATGAAAG TGTTCCAAAC AGGGAGGAAC AGCTTGATGA AGGTGTCCAA 
GACATCGAAG AGGATGCCAC CCAACCATAT GTTTTAAGTT TACCGACAGT GGGTGACATG 
GCTACACAGC CTTGTAGCAT GAGTGAGTCT GTCTCCGGGG ATGGTGCTAG TCAGTATTTA 
AATACAGTTG TTCCAGCTGT AGCTAGTGTT GCTTCTCTAC CCTGTAACCC AAGCATGCAA 
TTATCTGATG GCGAACCCAT GCAGCCTTCC AGTCTAAATG AGGCTGATGC AACCCAATGT 
CATGGATTTA GTATGCCCCT TAAAGAAGGT GATACAGTGA GCATTTCAGA AGAGAAAGTA 
TCAGTAAAGC AAGGTCCTTG CAGAGGTGAG AGTAGCATGA TATCTGAGCA GTTAGACCCT 
TCTAACAATG TTATGAGCAA GCACCCAGGT GAAGTTCTTG TGCATTCTGT TCCTCCATTG 
GAGAAAGCAA CAGAGCAGGT TGCAGAGCAG CCTCAAGCAG CCACCAGAAT GACAGAAGAA 
GACAGAGAAG AGAATAAAGG AAAGAGGAGA TCAACAGAAG GAGAAGATAG CAAGGATTCA 
GCAGAAGTTA CAACTTCCGT TACTAGGGGT AAAAAGAGAA CCTGGAAAAA GACTGCATCT 
CCAGCATCAG AGGAGACAAA AGGCAAAAAA ACAGCTAAAA TGCAGGTCAT GGAAAAAGCA 
TTACCTGAAG CAAAAATGGA AGAAGAATGT AAAGAAGAGC AGAACAATAA GCCAAACACT 
AGCTCTAGCC TTGCACGTTT AAAAACAGGT AGAAAGGGAA AACAAGATAT CGAAGAGCCT 
TTAGATTACA ATGTAGAAGG CTATAATGTG AGCGTGCAAC AGGTAGAAGA GGATATGAAA 
AGATTTACTT CAAGAAGATC AGTTAGAAAA CCAAAGGATG GAAATGATCA GGAGACTGGG 
TCAAGAAATT TACCAGAAAT AACCAGGCAT TCCAGTTTAG ACCTTAGTGA GTCAGTAGTG 
GAAAAAAAAG TAGATATGTC TTCTGATGAA CACATGAAGT ATCAGAAAGA TGATGAATTC 
TTAACTGCTA ATGGAACAAC TATAAATGTA AAAAGAAAGG GTGCAAAGAG AAATGTAAAG 
GAAGAGGAGA ACAAGTTTGT GGTAGATAAC ATTCCTGGAA AGCGACCAAC AAGGAATAAC 
AGGCCAAATG TTACAGAGAA AAATAATGAG GAAGCAGATG ATAAACAGAG GATTACAAGG 
AGGAATTCAA GGCAGCTGGA GATAGTAGAA ACCAAACCAG ACAATACAGA GATTTTAGAT 
TCTAAAAGAC AAAACCAGTC CAGAAAATCA CCAAATATAA AGAAAAATTT AAAAGTGGAT 
GAATCAAAAG AGACAGAAAA ATGTGAGGGC AAAACTAGAG AGAACACTCC GAAAAGGACA 
TCAAGGAGGA CAAAGAAAAA ATGCACAGAG GAACTGAACA TGGAAGAATG GGAAAAGTCA 
AAAGAAGAAG CAGACAGTAA ACAGAGGGTC ACAAGAAGGA ACTCCAGGCT ATCAGAGTCA 
GTAGAAAGCA TACCAGAGGA TACAGATATT GTAGATTCTG AGCAACAAGA CCAGTCCAGA 
AAATCTCGTA ATATAAAGAA GAATTTGAAA AGGGGCGAGG AAGAAAAACT TGAGGGCACC 
AACAGAGACA ACACTGAAAA ATGGACCTCA AGGAGGACAA GAAAGGAATG CAAAGAGAAA 
CTGAACATGG AGGACCATGA AAAGTCGAAA GTAGAACCAT CCATTGAACA GTCTGAACAA 
GAACATACTG AGGCTGAAGG ACAAGATCAA TCTAGAAAAT CTAGGAAGAC AAAGAAGGAT 
TTGCCACAAC CTGTTATCAA TGAGGAAGAA ACCCTTGTAG AAACCATCAG GAAAGACTCT 
GTAGGACGAA CATCAAGGAG AAGAAAGAAG AGTTGCATAG AGGAAGATCT GATGGACAGT 
GAGGTTCTAG TTATCAGTAA ACCTGAACAA GAAGATCCAC CCACAAAAAA TGTTCCTAGA 
AGTAAAAGGA AGAATTTGAA AGATGAACCT AAGGAGGGAG ATACTGAGCA TAATGTATTT 
GTATCAAAGA ATGAAAATCT AGATGAAATG CAGACCACTC AAAGGAAAAG CAATTCAAAA 
GATGGAGATG TGCAAAACGA TAATAAAACA CTGTGTGAAG TCGACTCCAA GTCTGGTAAT 
TCAGATATAA GAACAAGGAA GAAAAAAAAA GTTGTCACAG GGACTCAAAA AACCACAGAG 
GTAGAAGGGG AAATGCTAGA TGGTAGCATG ACAATAGACT CGCACGAAGA CGTTTCACCA 
ACTGGAATGA GAAAAAGCAG GCGGACAACA ACCAAGGAGG AATTACTCAA GTCTACCACT 
CTGGCTCCAG TAAGGAAGAG AGGGCAACAG CCAAAAACTG GAGGAGATGA GGTCAAGAGA 
AAAAAAGTGA ATGAAGAGTT TGAGCAGGAG AAAACAGTGG GTAGAAGGGG CCAAGCACGG 
CAACCAAAGC TGGAGGATGA AAATACTGAC GGCAACAGTT TTGGAAAGGA CTCGGCCGTT 
CCTTTTCCAT CACCCTCGGG GAACTCCCGT TTAAGGCCAA GCACATCCTT TGAAAGCCCG 
ACAGAAGGCC GGACACCAAG ACGTGCTGCT CGCAGCTTGA CCACAAGCCC CTACATGTCC 
CAACGGAGCA TTGCAGCAAA GGTTCTATTT ACAGGTGTGG TCGACCCTGC AGGTGAGGAG 
ACCATTCGTA ATCTAGGAGG GGAAGTTGCA GAATCCATAT TTGACTGCAC TCATCTTGTA 
ACTGACCGTA TCAGACGTAC CGTCAAATTT CTCTGTGCTC TTGCCAAGGG CATCCCTATT 
GTGACACTAG ACTGGCTTGA CAAGTGCAAA AAGAGCAGAT GTTTTTTGTC CCCTGCTCAA 
TTTCTGGTGA ACGATAAAGA ACAGGAGAAA AGCTTCAACT TCGATTTGTC AGAATCATTG 
CAAAAAGCCA AAAAGAAACT GCTGTTTGAG GGCTATGAAA TTCATGTCAC CCCGAATGTG 
AAACCTGAGC CTGAACACAT GAAAGATATT ATACAGTGCA GTGGGGCAAC TTTCCTTCCT 
AAAATGCCCA AAGTATACAA GGACAAATGC ATCATTGTAT CATGTAAGGA AGATGCCACT 
CGCTGCAAAT CTGCTCCCTC CACTATCCCT GTTACTTCTG CGGAATTTAT ATTAACTGGG 
ATCCTGCGTC AGGAAATAAA TCCACATGCT TATCTGCTAA GTACTGGACC TGAGGAAGCT 
TTGCCTACCC CTGCCAAACG GAGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdc1 ( XM_031890790.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746650.1 Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.

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CloneID	OXa64004
Clone ID Related Accession (Same CDS sequence)	XM_031890790.1 , XM_012969222.3
Accession Version	XM_031890790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGATA CTCAGCGCCT GCAGTGGGAC GATGAAGAAC AAGATGTGTC TTTTGATAGA 
GATAAACCAG TAGGAAATCT GCATATGTTC AATGGAATCC ATGGTCCAGC ACAAGACTTC 
CCTATTTACC CTGGTACGAA TATCATAGGA CGTCATACAA ATTGTGATGT GACTCTTCCA 
GCTCAGTCTG TCTCTAAGAA ACATGCTATT ATAGAGGTTG AGGCTGACTG CCATACCCTC 
TGTGACCAAG GAAGTCTAAA CAAAACTCGC CGTGGCAAGG CAGCCATTGC TCCTCATGTG 
AGATATGCTC TGTCTGATGG AGACTTATTG TTATTTGCTG ATGTAGCATG TCGTTACTCA 
TTCACAAAAA AACTAGAGGA GGAGGAGGCA GAGAATGAGG AAAGTGAAGA TGATATTTTG 
GTTCCTGGAA CACAGGCTAC TTTGTCAATT GAAAAAACTC CTGGGGTAGC AATACGCAGA 
ATGGGGAGAG GAGCAGTGCT GGCAATGGAC TCTGGTGATG AGGAAGAAGA GGAATTAAAA 
GGGAAAGATC TGAATTATGA AGCTAAAGGT GAGGAAGACA ATTACATACA AGAAGGTCTT 
AAAACATCCG GGGAGGAATG CAAGTCACCT GCCAGTGCTT CCTTTTCCCA CAACATGGAT 
ACTATTGTTC CTGAAAGTGA TGAGGAGAAC GACACATCTG TGTCTGAACC ACGTTTCCCT 
TCACTCAACC TCCGTTGTGA TAGTGATACA GACACTCATG ACACTCCCAT GAAGGCAGGC 
ACAAGCTACA CCCCCTCCCA AGATTGTGTG CTTTCTCCTT CTTTCAAAGA ACAAGTTGTG 
GGAGACCACA GTGAAATCAC AAAATCTACT TTGATTAGCA ACCAAGAGAT GTTTGCAGAT 
GAGCAAGTAA ACACTGAATC CAAAGTCTCG AAGTGTAGTC CTGATACAGC TGACAAAAAT 
GATAGAGAGA CAAAGGAAGA GAACAATACT CCTATAGTAA ACACTAATAT TGCTGAGGAA 
GGTGGAACAG GAGTAACAGA TGATCCATTG ATAGACGGTC TAAAAGTGGA AAACCAAAAA 
AAGGAAAATG ACTGTTATGA AACCCTGCAA GTTGACATCA AACAGCAAGA GAGTGTTAAA 
ATTATCTTGG ATAGTAATCA GGAGGAAAAC AGAACTTTGA AAGTAAAATT GGTAAACACA 
GGATCTGCAG ATATTATTCT AGACAAAAAT ATAGATGGGG AAGAAGGTTG CGTCAATACT 
AAGGAAATAG ACAACCAGAT CAGAGAAGGG GCTTTTCATT TGGACAGTGA CACAGATGTA 
GAGGAGAGCG ATACTTCAAT CCCTGGAATA AATGTACCCC GAGTGGAGAA TTCTGGGAAT 
AACACAGACA GTGATACAGA TACTGAAGAA GATAAACCTA AAATTTTGCA ACCTGCCATA 
CAACAGAATG TCGAGGAAGG GTTTAATTTG GACAGTGACA CGGATGTTGA GGGTAATGAC 
TCCAATTCTG TAAATAAAAG GTTGAAAAAT ACTGAAAAAG CAAAAATGAT CCAAGATAGT 
GATACTGATG CGGATGATTC GATTATGTCA AAGGTTGAAG AGAGGAGGAA CCCAGCAGGT 
ACAAAGAAAG ATTACAGTAG TACATCTCTA AACACAGAAG GTAAAAGGCA AGAAAATTAT 
CTATCCACCC TTGACAGTGA TACAGATGTT GAAGACATTG CTGATGGATC TTCCCCAAGT 
GTTGTGAAGA AGCAGGATAA GGCAGAAGCA GCTCATACTA ATGTAACCGA TACAACTGCT 
GGGGAGGAAA CTTGCACAGA TGCTGGAATA GAGAAGATTG TGGTGTCTGC AGATAGAAAT 
GAATGCCACA CAGGTGGAGT CACACAAGAA CAGGAGGCTA CATCTGAATT TCACCTAGAC 
AGTGATACAG ATATTGAGGA AGATGATAAT TTACTAGATA TGTCAAGTGC TAGTACTAGG 
GTAAAAGTCA CAGAGGTTCA CTCAGTTATC CTTGAAAAAC TAAGAACCTC ACCTGGAGAA 
AGAAAGGAAA AGAGGGCTAT TGAAATTGAT CTGGACAGCA ATACAGATGT GGATGAAGCT 
GATGAAACAG AAGTTGATAG GAAGGAAACA TTTCACATGG ACAGTGATAC AGATCTGGAG 
GAAAATGACA GTGGCCCTCC TGTGGTGGGT GGCATAGAAA AGAAATTTAC AGCCATAGCA 
CATTCTGATT GTACCTCAGA CACAGGAAGT AATGTGAAAA ATGTAACCTC TTCAATCACA 
GAAAATACAG AAAAAGGGGA GACTGATGGA ATAGACTGCG AAGATGCAGA ATATCAAATG 
ATGGCTACTC AGTGTTATCT AGAGCCCCAG GAAAAAGAGA CTGATATACA AGATGAAGAT 
GATTGTGCTG AGGAAGCTAC ACAAGCATTT ATCTTCAGTT CCACATGGGC TGAGCCGGAA 
CCATTTAAAC GTCCAGCCGA TCCTATTGGA GTTTTGCAAA TCTCAGCAGT GACTGTTAGT 
TCATCAGAGG AAGAACCAGA TGAAAATGCC ATTGCGGAGA CTCAGCCATT CTGCTGTGAG 
ATAAAGCAGC CAGAGGAACA CAGTGACCAA GAGCCAGCCA AAAAAAGTTC TATTGCTGAG 
GAAAAGAACA ATGTAGCTTG CTCTCTGCCT CAAGAAGAAA TATCTCAGGA TGATACCCAG 
CCAGTGTCTC AGTACTTGAA TAAAACAGCT TCAAGTAACT TGGTGGCACC AAATTTAACT 
TGCACAACAA ATTTTCAACA AGAAAAATAT CTTGATGGAA TTAATGAAGA AAAGGTCAAA 
CAGGCTAATG AAAGTGTTCC AAACAGGGAG GAACAGCTTG ATGAAGGTGT CCAAGACATC 
GAAGAGGATG CCACCCAACC ATATGTTTTA AGTTTACCGA CAGTGGGTGA CATGGCTACA 
CAGCCTTGTA GCATGAGTGA GTCTGTCTCC GGGGATGGTG CTAGTCAGTA TTTAAATACA 
GTTGTTCCAG CTGTAGCTAG TGTTGCTTCT CTACCCTGTA ACCCAAGCAT GCAATTATCT 
GATGGCGAAC CCATGCAGCC TTCCAGTCTA AATGAGGCTG ATGCAACCCA ATGTCATGGA 
TTTAGTATGC CCCTTAAAGA AGGTGATACA GTGAGCATTT CAGAAGAGAA AGTATCAGTA 
AAGCAAGGTC CTTGCAGAGG TGAGAGTAGC ATGATATCTG AGCAGTTAGA CCCTTCTAAC 
AATGTTATGA GCAAGCACCC AGGTGAAGTT CTTGTGCATT CTGTTCCTCC ATTGGAGAAA 
GCAACAGAGC AGGTTGCAGA GCAGCCTCAA GCAGCCACCA GAATGACAGA AGAAGACAGA 
GAAGAGAATA AAGGAAAGAG GAGATCAACA GAAGGAGAAG ATAGCAAGGA TTCAGCAGAA 
GTTACAACTT CCGTTACTAG GGGTAAAAAG AGAACCTGGA AAAAGACTGC ATCTCCAGCA 
TCAGAGGAGA CAAAAGGCAA AAAAACAGCT AAAATGCAGG TCATGGAAAA AGCATTACCT 
GAAGCAAAAA TGGAAGAAGA ATGTAAAGAA GAGCAGAACA ATAAGCCAAA CACTAGCTCT 
ATCCTTGCAC GTTTAAAAAC AGGTAGAAAG GGAAAACAAG ATATCGAAGA GCCTTTAGAT 
TACAATGTAG AAGGCTATAA TGTGAGCGTG CAACAGGTAG AAGAGGATAT GAAAAGATTT 
ACTTCAAGAA GATCAGTTAG AAAACCAAAG GATGGAAATG ATCAGGAGAC TGGGTCAAGA 
AATTTACCAG AAATAACCAG GCATTCCAGT TTAGACCTTA GTGAGTCAGT AGTGGAAAAA 
AAAGTAGATA TGTCTTCTGA TGAACACATG AAGTATCAGA AAGATGATGA ATTCTTAACT 
GCTAATGGAA CAACTATAAA TGTAAAAAGA AAGGGTGCAA AGAGAAATGT AAAGGAAGAG 
GAGAACAAGT TTGTGGTAGA TAACATTCCT GGAAAGCGAC CAACAAGGAA TAACAGGCCA 
AATGTTACAG AGAAAAATAA TGAGGAAGCA GATGATAAAC AGAGGATTAC AAGGAGGAAT 
TCAAGGCAGC TGGAGATAGT AGAAATCAAA CCAGACAATA CAGAGATTTT AGATTCTAAA 
AGACAAAACC AGTCCAGAAA ATCACCAAAT ATAAAGAAAA ATTTAAAAGT GGATGAATCA 
AAAGAGACAG AAAAATGTGA GGGCAAAACT AGAGAGAACA CTCCGAAAAG GACATCAAGG 
AGGACAAAGA AAAAATGCAC AGAGGAACTG AACATGGAAG AATGGGAAAA GTCAAAAGAA 
GAAGCAGACA GTAAACAGAG GGTCACAAGA AGGAACTCCA GGCTATCAGA GTCAGTAGAA 
AGCATACCAG AGGATACAGA TATTGTAGAT TCTGAGCAAC AAGACCAGTC CAGAAAATCT 
CGTAATATAA AGAAGAATTT GAAAAGGGGC GAGGAAGAAA AACTTGAGGG CACCAACAGA 
GACAACACTG AAAAATGGAC CTCAAGGAGG ACAAGAAAGG AATGCAAAGA GAAACTGAAC 
ATGGAGGACC ATGAAAAGTC GAAAGTAGAA CCATCCATTG AACAGTCTGA ACAAGAACAT 
ACTGAGGCTG AAGGACAAGA TCAATCTAGA AAATCTAGGA AGACAAAGAA GGATTTGCCA 
CAACCTGTTA TCAATGAGGA AGAAACCCTT GTAGAAACCA TCAGGAAAGA CTCTGTAGGA 
CGAACATCAA GGAGAAGAAA GAAGAGTTGC ATAGAGGAAG ATCTGATGGA CAGTGAGGTT 
CTAGTTATCA GTAAACCTGA ACAAGAAGAT CCACCCACAA AAAATGTTCC TAGAAGTAAA 
AGGAAGAATT TGAAAGATGA ACCTAAGGAG GGAGATACTG AGCATAATGT ATTTGTATCA 
AAGAATGAAA ATCTAGATGA AATGCAGACC ACTCAAAGGA AAAGCAATTC AAAAGATGGA 
GATGTGCAAA ACGATAATAA AACACTGTGT GAAGTCGACT CCAAGTCTGG TAATTCAGAT 
ATAAGAACAA GGAAGAAAAA AAAAGTTGTC ACAGGGACTC AAAAAACCAC AGAGGTAGAA 
GGGGAAATGC TAGATGGTAG CATGACAATA GACTCGCACG AAGACGTTTC ACCAACTGGA 
ATGAGAAAAA GCAGGCGGAC AACAACCAAG GAGGAATTAC TCAAGTCTAC CACTCTGGCT 
CCAGTAAGGA AGAGAGGGCA ACAGCCAAAA ACTGGAGGAG ATGAGGTCAA GAGAAAAAAA 
GTGAATGAAG AGTTTGAGCA GGAGAAAACA GTGGGTAGAA GGGGCCAAGC ACGGCAACCA 
AAGCTGGAGG ATGAAAATAC TGACGGCAAC AGTTTTGGAA AGGACTCGGC CGTTCCTTTT 
CCATCACCCT CGGGGAACTC CCGTTTAAGG CCAAGCACAT CCTTTGAAAG CCCGACAGAA 
GGCCGGACAC CAAGACGTGC TGCTCGCAGC TTGACCACAA GCCCCTACAT GTCCCAACGG 
AGCATTGCAG CAAAGGTTCT ATTTACAGGT GTGGTCGACC CTGCAGGTGA GGAGACCATT 
CGTAATCTAG GAGGGGAAGT TGCAGAATCC ATATTTGACT GCACTCATCT TGTAACTGAC 
CGTATCAGAC GTACCGTCAA ATTTCTCTGT GCTCTTGCCA AGGGCATCCC TATTGTGACA 
CTAGACTGGC TTGACAAGTG CAAAAAGAGC AGATGTTTTT TGTCCCCTGC TCAATTTCTG 
GTGAACGATA AAGAACAGGA GAAAAGCTTC AACTTCGATT TGTCAGAATC ATTGCAAAAA 
GCCAAAAAGA AACTGCTGTT TGAGGGCTAT GAAATTCATG TCACCCCGAA TGTGAAACCT 
GAGCCTGAAC ACATGAAAGA TATTATACAG TGCAGTGGGG CAACTTTCCT TCCTAAAATG 
CCCAAAGTAT ACAAGGACAA ATGCATCATT GTATCATGTA AGGAAGATGC CACTCGCTGC 
AAATCTGCTC CCTCCACTAT CCCTGTTACT TCTGCGGAAT TTATATTAAC TGGGATCCTG 
CGTCAGGAAA TAAATCCACA TGCTTATCTG CTAAGTACTG GACCTGAGGA AGCTTTGCCT 
ACCCCTGCCA AACGGAGGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746650.1
CDS	99..6242
Translation	MDDTQRLQWDDEEQDVSFDRDKPVGNLHMFNGIHGPAQDFPIYPGTNIIGRHTNCDVTLPAQSVSKKHAIIEVEADCHTLCDQGSLNKTRRGKAAIAPHVRYALSDGDLLLFADVACRYSFTKKLEEEEAENEESEDDILVPGTQATLSIEKTPGVAIRRMGRGAVLAMDSGDEEEEELKGKDLNYEAKGEEDNYIQEGLKTSGEECKSPASASFSHNMDTIVPESDEENDTSVSEPRFPSLNLRCDSDTDTHDTPMKAGTSYTPSQDCVLSPSFKEQVVGDHSEITKSTLISNQEMFADEQVNTESKVSKCSPDTADKNDRETKEENNTPIVNTNIAEEGGTGVTDDPLIDGLKVENQKKENDCYETLQVDIKQQESVKIILDSNQEENRTLKVKLVNTGSADIILDKNIDGEEGCVNTKEIDNQIREGAFHLDSDTDVEESDTSIPGINVPRVENSGNNTDSDTDTEEDKPKILQPAIQQNVEEGFNLDSDTDVEGNDSNSVNKRLKNTEKAKMIQDSDTDADDSIMSKVEERRNPAGTKKDYSSTSLNTEGKRQENYLSTLDSDTDVEDIADGSSPSVVKKQDKAEAAHTNVTDTTAGEETCTDAGIEKIVVSADRNECHTGGVTQEQEATSEFHLDSDTDIEEDDNLLDMSSASTRVKVTEVHSVILEKLRTSPGERKEKRAIEIDLDSNTDVDEADETEVDRKETFHMDSDTDLEENDSGPPVVGGIEKKFTAIAHSDCTSDTGSNVKNVTSSITENTEKGETDGIDCEDAEYQMMATQCYLEPQEKETDIQDEDDCAEEATQAFIFSSTWAEPEPFKRPADPIGVLQISAVTVSSSEEEPDENAIAETQPFCCEIKQPEEHSDQEPAKKSSIAEEKNNVACSLPQEEISQDDTQPVSQYLNKTASSNLVAPNLTCTTNFQQEKYLDGINEEKVKQANESVPNREEQLDEGVQDIEEDATQPYVLSLPTVGDMATQPCSMSESVSGDGASQYLNTVVPAVASVASLPCNPSMQLSDGEPMQPSSLNEADATQCHGFSMPLKEGDTVSISEEKVSVKQGPCRGESSMISEQLDPSNNVMSKHPGEVLVHSVPPLEKATEQVAEQPQAATRMTEEDREENKGKRRSTEGEDSKDSAEVTTSVTRGKKRTWKKTASPASEETKGKKTAKMQVMEKALPEAKMEEECKEEQNNKPNTSSILARLKTGRKGKQDIEEPLDYNVEGYNVSVQQVEEDMKRFTSRRSVRKPKDGNDQETGSRNLPEITRHSSLDLSESVVEKKVDMSSDEHMKYQKDDEFLTANGTTINVKRKGAKRNVKEEENKFVVDNIPGKRPTRNNRPNVTEKNNEEADDKQRITRRNSRQLEIVEIKPDNTEILDSKRQNQSRKSPNIKKNLKVDESKETEKCEGKTRENTPKRTSRRTKKKCTEELNMEEWEKSKEEADSKQRVTRRNSRLSESVESIPEDTDIVDSEQQDQSRKSRNIKKNLKRGEEEKLEGTNRDNTEKWTSRRTRKECKEKLNMEDHEKSKVEPSIEQSEQEHTEAEGQDQSRKSRKTKKDLPQPVINEEETLVETIRKDSVGRTSRRRKKSCIEEDLMDSEVLVISKPEQEDPPTKNVPRSKRKNLKDEPKEGDTEHNVFVSKNENLDEMQTTQRKSNSKDGDVQNDNKTLCEVDSKSGNSDIRTRKKKKVVTGTQKTTEVEGEMLDGSMTIDSHEDVSPTGMRKSRRTTTKEELLKSTTLAPVRKRGQQPKTGGDEVKRKKVNEEFEQEKTVGRRGQARQPKLEDENTDGNSFGKDSAVPFPSPSGNSRLRPSTSFESPTEGRTPRRAARSLTTSPYMSQRSIAAKVLFTGVVDPAGEETIRNLGGEVAESIFDCTHLVTDRIRRTVKFLCALAKGIPIVTLDWLDKCKKSRCFLSPAQFLVNDKEQEKSFNFDLSESLQKAKKKLLFEGYEIHVTPNVKPEPEHMKDIIQCSGATFLPKMPKVYKDKCIIVSCKEDATRCKSAPSTIPVTSAEFILTGILRQEINPHAYLLSTGPEEALPTPAKRRR

Target ORF information:

RefSeq Version	XM_031890790.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890790.1

ORF Insert Sequence:

ATGGACGATA CTCAGCGCCT GCAGTGGGAC GATGAAGAAC AAGATGTGTC TTTTGATAGA 
GATAAACCAG TAGGAAATCT GCATATGTTC AATGGAATCC ATGGTCCAGC ACAAGACTTC 
CCTATTTACC CTGGTACGAA TATCATAGGA CGTCATACAA ATTGTGATGT GACTCTTCCA 
GCTCAGTCTG TCTCTAAGAA ACATGCTATT ATAGAGGTTG AGGCTGACTG CCATACCCTC 
TGTGACCAAG GAAGTCTAAA CAAAACTCGC CGTGGCAAGG CAGCCATTGC TCCTCATGTG 
AGATATGCTC TGTCTGATGG AGACTTATTG TTATTTGCTG ATGTAGCATG TCGTTACTCA 
TTCACAAAAA AACTAGAGGA GGAGGAGGCA GAGAATGAGG AAAGTGAAGA TGATATTTTG 
GTTCCTGGAA CACAGGCTAC TTTGTCAATT GAAAAAACTC CTGGGGTAGC AATACGCAGA 
ATGGGGAGAG GAGCAGTGCT GGCAATGGAC TCTGGTGATG AGGAAGAAGA GGAATTAAAA 
GGGAAAGATC TGAATTATGA AGCTAAAGGT GAGGAAGACA ATTACATACA AGAAGGTCTT 
AAAACATCCG GGGAGGAATG CAAGTCACCT GCCAGTGCTT CCTTTTCCCA CAACATGGAT 
ACTATTGTTC CTGAAAGTGA TGAGGAGAAC GACACATCTG TGTCTGAACC ACGTTTCCCT 
TCACTCAACC TCCGTTGTGA TAGTGATACA GACACTCATG ACACTCCCAT GAAGGCAGGC 
ACAAGCTACA CCCCCTCCCA AGATTGTGTG CTTTCTCCTT CTTTCAAAGA ACAAGTTGTG 
GGAGACCACA GTGAAATCAC AAAATCTACT TTGATTAGCA ACCAAGAGAT GTTTGCAGAT 
GAGCAAGTAA ACACTGAATC CAAAGTCTCG AAGTGTAGTC CTGATACAGC TGACAAAAAT 
GATAGAGAGA CAAAGGAAGA GAACAATACT CCTATAGTAA ACACTAATAT TGCTGAGGAA 
GGTGGAACAG GAGTAACAGA TGATCCATTG ATAGACGGTC TAAAAGTGGA AAACCAAAAA 
AAGGAAAATG ACTGTTATGA AACCCTGCAA GTTGACATCA AACAGCAAGA GAGTGTTAAA 
ATTATCTTGG ATAGTAATCA GGAGGAAAAC AGAACTTTGA AAGTAAAATT GGTAAACACA 
GGATCTGCAG ATATTATTCT AGACAAAAAT ATAGATGGGG AAGAAGGTTG CGTCAATACT 
AAGGAAATAG ACAACCAGAT CAGAGAAGGG GCTTTTCATT TGGACAGTGA CACAGATGTA 
GAGGAGAGCG ATACTTCAAT CCCTGGAATA AATGTACCCC GAGTGGAGAA TTCTGGGAAT 
AACACAGACA GTGATACAGA TACTGAAGAA GATAAACCTA AAATTTTGCA ACCTGCCATA 
CAACAGAATG TCGAGGAAGG GTTTAATTTG GACAGTGACA CGGATGTTGA GGGTAATGAC 
TCCAATTCTG TAAATAAAAG GTTGAAAAAT ACTGAAAAAG CAAAAATGAT CCAAGATAGT 
GATACTGATG CGGATGATTC GATTATGTCA AAGGTTGAAG AGAGGAGGAA CCCAGCAGGT 
ACAAAGAAAG ATTACAGTAG TACATCTCTA AACACAGAAG GTAAAAGGCA AGAAAATTAT 
CTATCCACCC TTGACAGTGA TACAGATGTT GAAGACATTG CTGATGGATC TTCCCCAAGT 
GTTGTGAAGA AGCAGGATAA GGCAGAAGCA GCTCATACTA ATGTAACCGA TACAACTGCT 
GGGGAGGAAA CTTGCACAGA TGCTGGAATA GAGAAGATTG TGGTGTCTGC AGATAGAAAT 
GAATGCCACA CAGGTGGAGT CACACAAGAA CAGGAGGCTA CATCTGAATT TCACCTAGAC 
AGTGATACAG ATATTGAGGA AGATGATAAT TTACTAGATA TGTCAAGTGC TAGTACTAGG 
GTAAAAGTCA CAGAGGTTCA CTCAGTTATC CTTGAAAAAC TAAGAACCTC ACCTGGAGAA 
AGAAAGGAAA AGAGGGCTAT TGAAATTGAT CTGGACAGCA ATACAGATGT GGATGAAGCT 
GATGAAACAG AAGTTGATAG GAAGGAAACA TTTCACATGG ACAGTGATAC AGATCTGGAG 
GAAAATGACA GTGGCCCTCC TGTGGTGGGT GGCATAGAAA AGAAATTTAC AGCCATAGCA 
CATTCTGATT GTACCTCAGA CACAGGAAGT AATGTGAAAA ATGTAACCTC TTCAATCACA 
GAAAATACAG AAAAAGGGGA GACTGATGGA ATAGACTGCG AAGATGCAGA ATATCAAATG 
ATGGCTACTC AGTGTTATCT AGAGCCCCAG GAAAAAGAGA CTGATATACA AGATGAAGAT 
GATTGTGCTG AGGAAGCTAC ACAAGCATTT ATCTTCAGTT CCACATGGGC TGAGCCGGAA 
CCATTTAAAC GTCCAGCCGA TCCTATTGGA GTTTTGCAAA TCTCAGCAGT GACTGTTAGT 
TCATCAGAGG AAGAACCAGA TGAAAATGCC ATTGCGGAGA CTCAGCCATT CTGCTGTGAG 
ATAAAGCAGC CAGAGGAACA CAGTGACCAA GAGCCAGCCA AAAAAAGTTC TATTGCTGAG 
GAAAAGAACA ATGTAGCTTG CTCTCTGCCT CAAGAAGAAA TATCTCAGGA TGATACCCAG 
CCAGTGTCTC AGTACTTGAA TAAAACAGCT TCAAGTAACT TGGTGGCACC AAATTTAACT 
TGCACAACAA ATTTTCAACA AGAAAAATAT CTTGATGGAA TTAATGAAGA AAAGGTCAAA 
CAGGCTAATG AAAGTGTTCC AAACAGGGAG GAACAGCTTG ATGAAGGTGT CCAAGACATC 
GAAGAGGATG CCACCCAACC ATATGTTTTA AGTTTACCGA CAGTGGGTGA CATGGCTACA 
CAGCCTTGTA GCATGAGTGA GTCTGTCTCC GGGGATGGTG CTAGTCAGTA TTTAAATACA 
GTTGTTCCAG CTGTAGCTAG TGTTGCTTCT CTACCCTGTA ACCCAAGCAT GCAATTATCT 
GATGGCGAAC CCATGCAGCC TTCCAGTCTA AATGAGGCTG ATGCAACCCA ATGTCATGGA 
TTTAGTATGC CCCTTAAAGA AGGTGATACA GTGAGCATTT CAGAAGAGAA AGTATCAGTA 
AAGCAAGGTC CTTGCAGAGG TGAGAGTAGC ATGATATCTG AGCAGTTAGA CCCTTCTAAC 
AATGTTATGA GCAAGCACCC AGGTGAAGTT CTTGTGCATT CTGTTCCTCC ATTGGAGAAA 
GCAACAGAGC AGGTTGCAGA GCAGCCTCAA GCAGCCACCA GAATGACAGA AGAAGACAGA 
GAAGAGAATA AAGGAAAGAG GAGATCAACA GAAGGAGAAG ATAGCAAGGA TTCAGCAGAA 
GTTACAACTT CCGTTACTAG GGGTAAAAAG AGAACCTGGA AAAAGACTGC ATCTCCAGCA 
TCAGAGGAGA CAAAAGGCAA AAAAACAGCT AAAATGCAGG TCATGGAAAA AGCATTACCT 
GAAGCAAAAA TGGAAGAAGA ATGTAAAGAA GAGCAGAACA ATAAGCCAAA CACTAGCTCT 
ATCCTTGCAC GTTTAAAAAC AGGTAGAAAG GGAAAACAAG ATATCGAAGA GCCTTTAGAT 
TACAATGTAG AAGGCTATAA TGTGAGCGTG CAACAGGTAG AAGAGGATAT GAAAAGATTT 
ACTTCAAGAA GATCAGTTAG AAAACCAAAG GATGGAAATG ATCAGGAGAC TGGGTCAAGA 
AATTTACCAG AAATAACCAG GCATTCCAGT TTAGACCTTA GTGAGTCAGT AGTGGAAAAA 
AAAGTAGATA TGTCTTCTGA TGAACACATG AAGTATCAGA AAGATGATGA ATTCTTAACT 
GCTAATGGAA CAACTATAAA TGTAAAAAGA AAGGGTGCAA AGAGAAATGT AAAGGAAGAG 
GAGAACAAGT TTGTGGTAGA TAACATTCCT GGAAAGCGAC CAACAAGGAA TAACAGGCCA 
AATGTTACAG AGAAAAATAA TGAGGAAGCA GATGATAAAC AGAGGATTAC AAGGAGGAAT 
TCAAGGCAGC TGGAGATAGT AGAAATCAAA CCAGACAATA CAGAGATTTT AGATTCTAAA 
AGACAAAACC AGTCCAGAAA ATCACCAAAT ATAAAGAAAA ATTTAAAAGT GGATGAATCA 
AAAGAGACAG AAAAATGTGA GGGCAAAACT AGAGAGAACA CTCCGAAAAG GACATCAAGG 
AGGACAAAGA AAAAATGCAC AGAGGAACTG AACATGGAAG AATGGGAAAA GTCAAAAGAA 
GAAGCAGACA GTAAACAGAG GGTCACAAGA AGGAACTCCA GGCTATCAGA GTCAGTAGAA 
AGCATACCAG AGGATACAGA TATTGTAGAT TCTGAGCAAC AAGACCAGTC CAGAAAATCT 
CGTAATATAA AGAAGAATTT GAAAAGGGGC GAGGAAGAAA AACTTGAGGG CACCAACAGA 
GACAACACTG AAAAATGGAC CTCAAGGAGG ACAAGAAAGG AATGCAAAGA GAAACTGAAC 
ATGGAGGACC ATGAAAAGTC GAAAGTAGAA CCATCCATTG AACAGTCTGA ACAAGAACAT 
ACTGAGGCTG AAGGACAAGA TCAATCTAGA AAATCTAGGA AGACAAAGAA GGATTTGCCA 
CAACCTGTTA TCAATGAGGA AGAAACCCTT GTAGAAACCA TCAGGAAAGA CTCTGTAGGA 
CGAACATCAA GGAGAAGAAA GAAGAGTTGC ATAGAGGAAG ATCTGATGGA CAGTGAGGTT 
CTAGTTATCA GTAAACCTGA ACAAGAAGAT CCACCCACAA AAAATGTTCC TAGAAGTAAA 
AGGAAGAATT TGAAAGATGA ACCTAAGGAG GGAGATACTG AGCATAATGT ATTTGTATCA 
AAGAATGAAA ATCTAGATGA AATGCAGACC ACTCAAAGGA AAAGCAATTC AAAAGATGGA 
GATGTGCAAA ACGATAATAA AACACTGTGT GAAGTCGACT CCAAGTCTGG TAATTCAGAT 
ATAAGAACAA GGAAGAAAAA AAAAGTTGTC ACAGGGACTC AAAAAACCAC AGAGGTAGAA 
GGGGAAATGC TAGATGGTAG CATGACAATA GACTCGCACG AAGACGTTTC ACCAACTGGA 
ATGAGAAAAA GCAGGCGGAC AACAACCAAG GAGGAATTAC TCAAGTCTAC CACTCTGGCT 
CCAGTAAGGA AGAGAGGGCA ACAGCCAAAA ACTGGAGGAG ATGAGGTCAA GAGAAAAAAA 
GTGAATGAAG AGTTTGAGCA GGAGAAAACA GTGGGTAGAA GGGGCCAAGC ACGGCAACCA 
AAGCTGGAGG ATGAAAATAC TGACGGCAAC AGTTTTGGAA AGGACTCGGC CGTTCCTTTT 
CCATCACCCT CGGGGAACTC CCGTTTAAGG CCAAGCACAT CCTTTGAAAG CCCGACAGAA 
GGCCGGACAC CAAGACGTGC TGCTCGCAGC TTGACCACAA GCCCCTACAT GTCCCAACGG 
AGCATTGCAG CAAAGGTTCT ATTTACAGGT GTGGTCGACC CTGCAGGTGA GGAGACCATT 
CGTAATCTAG GAGGGGAAGT TGCAGAATCC ATATTTGACT GCACTCATCT TGTAACTGAC 
CGTATCAGAC GTACCGTCAA ATTTCTCTGT GCTCTTGCCA AGGGCATCCC TATTGTGACA 
CTAGACTGGC TTGACAAGTG CAAAAAGAGC AGATGTTTTT TGTCCCCTGC TCAATTTCTG 
GTGAACGATA AAGAACAGGA GAAAAGCTTC AACTTCGATT TGTCAGAATC ATTGCAAAAA 
GCCAAAAAGA AACTGCTGTT TGAGGGCTAT GAAATTCATG TCACCCCGAA TGTGAAACCT 
GAGCCTGAAC ACATGAAAGA TATTATACAG TGCAGTGGGG CAACTTTCCT TCCTAAAATG 
CCCAAAGTAT ACAAGGACAA ATGCATCATT GTATCATGTA AGGAAGATGC CACTCGCTGC 
AAATCTGCTC CCTCCACTAT CCCTGTTACT TCTGCGGAAT TTATATTAAC TGGGATCCTG 
CGTCAGGAAA TAAATCCACA TGCTTATCTG CTAAGTACTG GACCTGAGGA AGCTTTGCCT 
ACCCCTGCCA AACGGAGGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdc1 ( XM_012969222.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824676.2 Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.

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CloneID	OXa64004
Clone ID Related Accession (Same CDS sequence)	XM_031890790.1 , XM_012969222.3
Accession Version	XM_012969222.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGATA CTCAGCGCCT GCAGTGGGAC GATGAAGAAC AAGATGTGTC TTTTGATAGA 
GATAAACCAG TAGGAAATCT GCATATGTTC AATGGAATCC ATGGTCCAGC ACAAGACTTC 
CCTATTTACC CTGGTACGAA TATCATAGGA CGTCATACAA ATTGTGATGT GACTCTTCCA 
GCTCAGTCTG TCTCTAAGAA ACATGCTATT ATAGAGGTTG AGGCTGACTG CCATACCCTC 
TGTGACCAAG GAAGTCTAAA CAAAACTCGC CGTGGCAAGG CAGCCATTGC TCCTCATGTG 
AGATATGCTC TGTCTGATGG AGACTTATTG TTATTTGCTG ATGTAGCATG TCGTTACTCA 
TTCACAAAAA AACTAGAGGA GGAGGAGGCA GAGAATGAGG AAAGTGAAGA TGATATTTTG 
GTTCCTGGAA CACAGGCTAC TTTGTCAATT GAAAAAACTC CTGGGGTAGC AATACGCAGA 
ATGGGGAGAG GAGCAGTGCT GGCAATGGAC TCTGGTGATG AGGAAGAAGA GGAATTAAAA 
GGGAAAGATC TGAATTATGA AGCTAAAGGT GAGGAAGACA ATTACATACA AGAAGGTCTT 
AAAACATCCG GGGAGGAATG CAAGTCACCT GCCAGTGCTT CCTTTTCCCA CAACATGGAT 
ACTATTGTTC CTGAAAGTGA TGAGGAGAAC GACACATCTG TGTCTGAACC ACGTTTCCCT 
TCACTCAACC TCCGTTGTGA TAGTGATACA GACACTCATG ACACTCCCAT GAAGGCAGGC 
ACAAGCTACA CCCCCTCCCA AGATTGTGTG CTTTCTCCTT CTTTCAAAGA ACAAGTTGTG 
GGAGACCACA GTGAAATCAC AAAATCTACT TTGATTAGCA ACCAAGAGAT GTTTGCAGAT 
GAGCAAGTAA ACACTGAATC CAAAGTCTCG AAGTGTAGTC CTGATACAGC TGACAAAAAT 
GATAGAGAGA CAAAGGAAGA GAACAATACT CCTATAGTAA ACACTAATAT TGCTGAGGAA 
GGTGGAACAG GAGTAACAGA TGATCCATTG ATAGACGGTC TAAAAGTGGA AAACCAAAAA 
AAGGAAAATG ACTGTTATGA AACCCTGCAA GTTGACATCA AACAGCAAGA GAGTGTTAAA 
ATTATCTTGG ATAGTAATCA GGAGGAAAAC AGAACTTTGA AAGTAAAATT GGTAAACACA 
GGATCTGCAG ATATTATTCT AGACAAAAAT ATAGATGGGG AAGAAGGTTG CGTCAATACT 
AAGGAAATAG ACAACCAGAT CAGAGAAGGG GCTTTTCATT TGGACAGTGA CACAGATGTA 
GAGGAGAGCG ATACTTCAAT CCCTGGAATA AATGTACCCC GAGTGGAGAA TTCTGGGAAT 
AACACAGACA GTGATACAGA TACTGAAGAA GATAAACCTA AAATTTTGCA ACCTGCCATA 
CAACAGAATG TCGAGGAAGG GTTTAATTTG GACAGTGACA CGGATGTTGA GGGTAATGAC 
TCCAATTCTG TAAATAAAAG GTTGAAAAAT ACTGAAAAAG CAAAAATGAT CCAAGATAGT 
GATACTGATG CGGATGATTC GATTATGTCA AAGGTTGAAG AGAGGAGGAA CCCAGCAGGT 
ACAAAGAAAG ATTACAGTAG TACATCTCTA AACACAGAAG GTAAAAGGCA AGAAAATTAT 
CTATCCACCC TTGACAGTGA TACAGATGTT GAAGACATTG CTGATGGATC TTCCCCAAGT 
GTTGTGAAGA AGCAGGATAA GGCAGAAGCA GCTCATACTA ATGTAACCGA TACAACTGCT 
GGGGAGGAAA CTTGCACAGA TGCTGGAATA GAGAAGATTG TGGTGTCTGC AGATAGAAAT 
GAATGCCACA CAGGTGGAGT CACACAAGAA CAGGAGGCTA CATCTGAATT TCACCTAGAC 
AGTGATACAG ATATTGAGGA AGATGATAAT TTACTAGATA TGTCAAGTGC TAGTACTAGG 
GTAAAAGTCA CAGAGGTTCA CTCAGTTATC CTTGAAAAAC TAAGAACCTC ACCTGGAGAA 
AGAAAGGAAA AGAGGGCTAT TGAAATTGAT CTGGACAGCA ATACAGATGT GGATGAAGCT 
GATGAAACAG AAGTTGATAG GAAGGAAACA TTTCACATGG ACAGTGATAC AGATCTGGAG 
GAAAATGACA GTGGCCCTCC TGTGGTGGGT GGCATAGAAA AGAAATTTAC AGCCATAGCA 
CATTCTGATT GTACCTCAGA CACAGGAAGT AATGTGAAAA ATGTAACCTC TTCAATCACA 
GAAAATACAG AAAAAGGGGA GACTGATGGA ATAGACTGCG AAGATGCAGA ATATCAAATG 
ATGGCTACTC AGTGTTATCT AGAGCCCCAG GAAAAAGAGA CTGATATACA AGATGAAGAT 
GATTGTGCTG AGGAAGCTAC ACAAGCATTT ATCTTCAGTT CCACATGGGC TGAGCCGGAA 
CCATTTAAAC GTCCAGCCGA TCCTATTGGA GTTTTGCAAA TCTCAGCAGT GACTGTTAGT 
TCATCAGAGG AAGAACCAGA TGAAAATGCC ATTGCGGAGA CTCAGCCATT CTGCTGTGAG 
ATAAAGCAGC CAGAGGAACA CAGTGACCAA GAGCCAGCCA AAAAAAGTTC TATTGCTGAG 
GAAAAGAACA ATGTAGCTTG CTCTCTGCCT CAAGAAGAAA TATCTCAGGA TGATACCCAG 
CCAGTGTCTC AGTACTTGAA TAAAACAGCT TCAAGTAACT TGGTGGCACC AAATTTAACT 
TGCACAACAA ATTTTCAACA AGAAAAATAT CTTGATGGAA TTAATGAAGA AAAGGTCAAA 
CAGGCTAATG AAAGTGTTCC AAACAGGGAG GAACAGCTTG ATGAAGGTGT CCAAGACATC 
GAAGAGGATG CCACCCAACC ATATGTTTTA AGTTTACCGA CAGTGGGTGA CATGGCTACA 
CAGCCTTGTA GCATGAGTGA GTCTGTCTCC GGGGATGGTG CTAGTCAGTA TTTAAATACA 
GTTGTTCCAG CTGTAGCTAG TGTTGCTTCT CTACCCTGTA ACCCAAGCAT GCAATTATCT 
GATGGCGAAC CCATGCAGCC TTCCAGTCTA AATGAGGCTG ATGCAACCCA ATGTCATGGA 
TTTAGTATGC CCCTTAAAGA AGGTGATACA GTGAGCATTT CAGAAGAGAA AGTATCAGTA 
AAGCAAGGTC CTTGCAGAGG TGAGAGTAGC ATGATATCTG AGCAGTTAGA CCCTTCTAAC 
AATGTTATGA GCAAGCACCC AGGTGAAGTT CTTGTGCATT CTGTTCCTCC ATTGGAGAAA 
GCAACAGAGC AGGTTGCAGA GCAGCCTCAA GCAGCCACCA GAATGACAGA AGAAGACAGA 
GAAGAGAATA AAGGAAAGAG GAGATCAACA GAAGGAGAAG ATAGCAAGGA TTCAGCAGAA 
GTTACAACTT CCGTTACTAG GGGTAAAAAG AGAACCTGGA AAAAGACTGC ATCTCCAGCA 
TCAGAGGAGA CAAAAGGCAA AAAAACAGCT AAAATGCAGG TCATGGAAAA AGCATTACCT 
GAAGCAAAAA TGGAAGAAGA ATGTAAAGAA GAGCAGAACA ATAAGCCAAA CACTAGCTCT 
ATCCTTGCAC GTTTAAAAAC AGGTAGAAAG GGAAAACAAG ATATCGAAGA GCCTTTAGAT 
TACAATGTAG AAGGCTATAA TGTGAGCGTG CAACAGGTAG AAGAGGATAT GAAAAGATTT 
ACTTCAAGAA GATCAGTTAG AAAACCAAAG GATGGAAATG ATCAGGAGAC TGGGTCAAGA 
AATTTACCAG AAATAACCAG GCATTCCAGT TTAGACCTTA GTGAGTCAGT AGTGGAAAAA 
AAAGTAGATA TGTCTTCTGA TGAACACATG AAGTATCAGA AAGATGATGA ATTCTTAACT 
GCTAATGGAA CAACTATAAA TGTAAAAAGA AAGGGTGCAA AGAGAAATGT AAAGGAAGAG 
GAGAACAAGT TTGTGGTAGA TAACATTCCT GGAAAGCGAC CAACAAGGAA TAACAGGCCA 
AATGTTACAG AGAAAAATAA TGAGGAAGCA GATGATAAAC AGAGGATTAC AAGGAGGAAT 
TCAAGGCAGC TGGAGATAGT AGAAATCAAA CCAGACAATA CAGAGATTTT AGATTCTAAA 
AGACAAAACC AGTCCAGAAA ATCACCAAAT ATAAAGAAAA ATTTAAAAGT GGATGAATCA 
AAAGAGACAG AAAAATGTGA GGGCAAAACT AGAGAGAACA CTCCGAAAAG GACATCAAGG 
AGGACAAAGA AAAAATGCAC AGAGGAACTG AACATGGAAG AATGGGAAAA GTCAAAAGAA 
GAAGCAGACA GTAAACAGAG GGTCACAAGA AGGAACTCCA GGCTATCAGA GTCAGTAGAA 
AGCATACCAG AGGATACAGA TATTGTAGAT TCTGAGCAAC AAGACCAGTC CAGAAAATCT 
CGTAATATAA AGAAGAATTT GAAAAGGGGC GAGGAAGAAA AACTTGAGGG CACCAACAGA 
GACAACACTG AAAAATGGAC CTCAAGGAGG ACAAGAAAGG AATGCAAAGA GAAACTGAAC 
ATGGAGGACC ATGAAAAGTC GAAAGTAGAA CCATCCATTG AACAGTCTGA ACAAGAACAT 
ACTGAGGCTG AAGGACAAGA TCAATCTAGA AAATCTAGGA AGACAAAGAA GGATTTGCCA 
CAACCTGTTA TCAATGAGGA AGAAACCCTT GTAGAAACCA TCAGGAAAGA CTCTGTAGGA 
CGAACATCAA GGAGAAGAAA GAAGAGTTGC ATAGAGGAAG ATCTGATGGA CAGTGAGGTT 
CTAGTTATCA GTAAACCTGA ACAAGAAGAT CCACCCACAA AAAATGTTCC TAGAAGTAAA 
AGGAAGAATT TGAAAGATGA ACCTAAGGAG GGAGATACTG AGCATAATGT ATTTGTATCA 
AAGAATGAAA ATCTAGATGA AATGCAGACC ACTCAAAGGA AAAGCAATTC AAAAGATGGA 
GATGTGCAAA ACGATAATAA AACACTGTGT GAAGTCGACT CCAAGTCTGG TAATTCAGAT 
ATAAGAACAA GGAAGAAAAA AAAAGTTGTC ACAGGGACTC AAAAAACCAC AGAGGTAGAA 
GGGGAAATGC TAGATGGTAG CATGACAATA GACTCGCACG AAGACGTTTC ACCAACTGGA 
ATGAGAAAAA GCAGGCGGAC AACAACCAAG GAGGAATTAC TCAAGTCTAC CACTCTGGCT 
CCAGTAAGGA AGAGAGGGCA ACAGCCAAAA ACTGGAGGAG ATGAGGTCAA GAGAAAAAAA 
GTGAATGAAG AGTTTGAGCA GGAGAAAACA GTGGGTAGAA GGGGCCAAGC ACGGCAACCA 
AAGCTGGAGG ATGAAAATAC TGACGGCAAC AGTTTTGGAA AGGACTCGGC CGTTCCTTTT 
CCATCACCCT CGGGGAACTC CCGTTTAAGG CCAAGCACAT CCTTTGAAAG CCCGACAGAA 
GGCCGGACAC CAAGACGTGC TGCTCGCAGC TTGACCACAA GCCCCTACAT GTCCCAACGG 
AGCATTGCAG CAAAGGTTCT ATTTACAGGT GTGGTCGACC CTGCAGGTGA GGAGACCATT 
CGTAATCTAG GAGGGGAAGT TGCAGAATCC ATATTTGACT GCACTCATCT TGTAACTGAC 
CGTATCAGAC GTACCGTCAA ATTTCTCTGT GCTCTTGCCA AGGGCATCCC TATTGTGACA 
CTAGACTGGC TTGACAAGTG CAAAAAGAGC AGATGTTTTT TGTCCCCTGC TCAATTTCTG 
GTGAACGATA AAGAACAGGA GAAAAGCTTC AACTTCGATT TGTCAGAATC ATTGCAAAAA 
GCCAAAAAGA AACTGCTGTT TGAGGGCTAT GAAATTCATG TCACCCCGAA TGTGAAACCT 
GAGCCTGAAC ACATGAAAGA TATTATACAG TGCAGTGGGG CAACTTTCCT TCCTAAAATG 
CCCAAAGTAT ACAAGGACAA ATGCATCATT GTATCATGTA AGGAAGATGC CACTCGCTGC 
AAATCTGCTC CCTCCACTAT CCCTGTTACT TCTGCGGAAT TTATATTAAC TGGGATCCTG 
CGTCAGGAAA TAAATCCACA TGCTTATCTG CTAAGTACTG GACCTGAGGA AGCTTTGCCT 
ACCCCTGCCA AACGGAGGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824676.2
CDS	232..6375
Translation	MDDTQRLQWDDEEQDVSFDRDKPVGNLHMFNGIHGPAQDFPIYPGTNIIGRHTNCDVTLPAQSVSKKHAIIEVEADCHTLCDQGSLNKTRRGKAAIAPHVRYALSDGDLLLFADVACRYSFTKKLEEEEAENEESEDDILVPGTQATLSIEKTPGVAIRRMGRGAVLAMDSGDEEEEELKGKDLNYEAKGEEDNYIQEGLKTSGEECKSPASASFSHNMDTIVPESDEENDTSVSEPRFPSLNLRCDSDTDTHDTPMKAGTSYTPSQDCVLSPSFKEQVVGDHSEITKSTLISNQEMFADEQVNTESKVSKCSPDTADKNDRETKEENNTPIVNTNIAEEGGTGVTDDPLIDGLKVENQKKENDCYETLQVDIKQQESVKIILDSNQEENRTLKVKLVNTGSADIILDKNIDGEEGCVNTKEIDNQIREGAFHLDSDTDVEESDTSIPGINVPRVENSGNNTDSDTDTEEDKPKILQPAIQQNVEEGFNLDSDTDVEGNDSNSVNKRLKNTEKAKMIQDSDTDADDSIMSKVEERRNPAGTKKDYSSTSLNTEGKRQENYLSTLDSDTDVEDIADGSSPSVVKKQDKAEAAHTNVTDTTAGEETCTDAGIEKIVVSADRNECHTGGVTQEQEATSEFHLDSDTDIEEDDNLLDMSSASTRVKVTEVHSVILEKLRTSPGERKEKRAIEIDLDSNTDVDEADETEVDRKETFHMDSDTDLEENDSGPPVVGGIEKKFTAIAHSDCTSDTGSNVKNVTSSITENTEKGETDGIDCEDAEYQMMATQCYLEPQEKETDIQDEDDCAEEATQAFIFSSTWAEPEPFKRPADPIGVLQISAVTVSSSEEEPDENAIAETQPFCCEIKQPEEHSDQEPAKKSSIAEEKNNVACSLPQEEISQDDTQPVSQYLNKTASSNLVAPNLTCTTNFQQEKYLDGINEEKVKQANESVPNREEQLDEGVQDIEEDATQPYVLSLPTVGDMATQPCSMSESVSGDGASQYLNTVVPAVASVASLPCNPSMQLSDGEPMQPSSLNEADATQCHGFSMPLKEGDTVSISEEKVSVKQGPCRGESSMISEQLDPSNNVMSKHPGEVLVHSVPPLEKATEQVAEQPQAATRMTEEDREENKGKRRSTEGEDSKDSAEVTTSVTRGKKRTWKKTASPASEETKGKKTAKMQVMEKALPEAKMEEECKEEQNNKPNTSSILARLKTGRKGKQDIEEPLDYNVEGYNVSVQQVEEDMKRFTSRRSVRKPKDGNDQETGSRNLPEITRHSSLDLSESVVEKKVDMSSDEHMKYQKDDEFLTANGTTINVKRKGAKRNVKEEENKFVVDNIPGKRPTRNNRPNVTEKNNEEADDKQRITRRNSRQLEIVEIKPDNTEILDSKRQNQSRKSPNIKKNLKVDESKETEKCEGKTRENTPKRTSRRTKKKCTEELNMEEWEKSKEEADSKQRVTRRNSRLSESVESIPEDTDIVDSEQQDQSRKSRNIKKNLKRGEEEKLEGTNRDNTEKWTSRRTRKECKEKLNMEDHEKSKVEPSIEQSEQEHTEAEGQDQSRKSRKTKKDLPQPVINEEETLVETIRKDSVGRTSRRRKKSCIEEDLMDSEVLVISKPEQEDPPTKNVPRSKRKNLKDEPKEGDTEHNVFVSKNENLDEMQTTQRKSNSKDGDVQNDNKTLCEVDSKSGNSDIRTRKKKKVVTGTQKTTEVEGEMLDGSMTIDSHEDVSPTGMRKSRRTTTKEELLKSTTLAPVRKRGQQPKTGGDEVKRKKVNEEFEQEKTVGRRGQARQPKLEDENTDGNSFGKDSAVPFPSPSGNSRLRPSTSFESPTEGRTPRRAARSLTTSPYMSQRSIAAKVLFTGVVDPAGEETIRNLGGEVAESIFDCTHLVTDRIRRTVKFLCALAKGIPIVTLDWLDKCKKSRCFLSPAQFLVNDKEQEKSFNFDLSESLQKAKKKLLFEGYEIHVTPNVKPEPEHMKDIIQCSGATFLPKMPKVYKDKCIIVSCKEDATRCKSAPSTIPVTSAEFILTGILRQEINPHAYLLSTGPEEALPTPAKRRR

Target ORF information:

RefSeq Version	XM_012969222.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969222.3

ORF Insert Sequence:

ATGGACGATA CTCAGCGCCT GCAGTGGGAC GATGAAGAAC AAGATGTGTC TTTTGATAGA 
GATAAACCAG TAGGAAATCT GCATATGTTC AATGGAATCC ATGGTCCAGC ACAAGACTTC 
CCTATTTACC CTGGTACGAA TATCATAGGA CGTCATACAA ATTGTGATGT GACTCTTCCA 
GCTCAGTCTG TCTCTAAGAA ACATGCTATT ATAGAGGTTG AGGCTGACTG CCATACCCTC 
TGTGACCAAG GAAGTCTAAA CAAAACTCGC CGTGGCAAGG CAGCCATTGC TCCTCATGTG 
AGATATGCTC TGTCTGATGG AGACTTATTG TTATTTGCTG ATGTAGCATG TCGTTACTCA 
TTCACAAAAA AACTAGAGGA GGAGGAGGCA GAGAATGAGG AAAGTGAAGA TGATATTTTG 
GTTCCTGGAA CACAGGCTAC TTTGTCAATT GAAAAAACTC CTGGGGTAGC AATACGCAGA 
ATGGGGAGAG GAGCAGTGCT GGCAATGGAC TCTGGTGATG AGGAAGAAGA GGAATTAAAA 
GGGAAAGATC TGAATTATGA AGCTAAAGGT GAGGAAGACA ATTACATACA AGAAGGTCTT 
AAAACATCCG GGGAGGAATG CAAGTCACCT GCCAGTGCTT CCTTTTCCCA CAACATGGAT 
ACTATTGTTC CTGAAAGTGA TGAGGAGAAC GACACATCTG TGTCTGAACC ACGTTTCCCT 
TCACTCAACC TCCGTTGTGA TAGTGATACA GACACTCATG ACACTCCCAT GAAGGCAGGC 
ACAAGCTACA CCCCCTCCCA AGATTGTGTG CTTTCTCCTT CTTTCAAAGA ACAAGTTGTG 
GGAGACCACA GTGAAATCAC AAAATCTACT TTGATTAGCA ACCAAGAGAT GTTTGCAGAT 
GAGCAAGTAA ACACTGAATC CAAAGTCTCG AAGTGTAGTC CTGATACAGC TGACAAAAAT 
GATAGAGAGA CAAAGGAAGA GAACAATACT CCTATAGTAA ACACTAATAT TGCTGAGGAA 
GGTGGAACAG GAGTAACAGA TGATCCATTG ATAGACGGTC TAAAAGTGGA AAACCAAAAA 
AAGGAAAATG ACTGTTATGA AACCCTGCAA GTTGACATCA AACAGCAAGA GAGTGTTAAA 
ATTATCTTGG ATAGTAATCA GGAGGAAAAC AGAACTTTGA AAGTAAAATT GGTAAACACA 
GGATCTGCAG ATATTATTCT AGACAAAAAT ATAGATGGGG AAGAAGGTTG CGTCAATACT 
AAGGAAATAG ACAACCAGAT CAGAGAAGGG GCTTTTCATT TGGACAGTGA CACAGATGTA 
GAGGAGAGCG ATACTTCAAT CCCTGGAATA AATGTACCCC GAGTGGAGAA TTCTGGGAAT 
AACACAGACA GTGATACAGA TACTGAAGAA GATAAACCTA AAATTTTGCA ACCTGCCATA 
CAACAGAATG TCGAGGAAGG GTTTAATTTG GACAGTGACA CGGATGTTGA GGGTAATGAC 
TCCAATTCTG TAAATAAAAG GTTGAAAAAT ACTGAAAAAG CAAAAATGAT CCAAGATAGT 
GATACTGATG CGGATGATTC GATTATGTCA AAGGTTGAAG AGAGGAGGAA CCCAGCAGGT 
ACAAAGAAAG ATTACAGTAG TACATCTCTA AACACAGAAG GTAAAAGGCA AGAAAATTAT 
CTATCCACCC TTGACAGTGA TACAGATGTT GAAGACATTG CTGATGGATC TTCCCCAAGT 
GTTGTGAAGA AGCAGGATAA GGCAGAAGCA GCTCATACTA ATGTAACCGA TACAACTGCT 
GGGGAGGAAA CTTGCACAGA TGCTGGAATA GAGAAGATTG TGGTGTCTGC AGATAGAAAT 
GAATGCCACA CAGGTGGAGT CACACAAGAA CAGGAGGCTA CATCTGAATT TCACCTAGAC 
AGTGATACAG ATATTGAGGA AGATGATAAT TTACTAGATA TGTCAAGTGC TAGTACTAGG 
GTAAAAGTCA CAGAGGTTCA CTCAGTTATC CTTGAAAAAC TAAGAACCTC ACCTGGAGAA 
AGAAAGGAAA AGAGGGCTAT TGAAATTGAT CTGGACAGCA ATACAGATGT GGATGAAGCT 
GATGAAACAG AAGTTGATAG GAAGGAAACA TTTCACATGG ACAGTGATAC AGATCTGGAG 
GAAAATGACA GTGGCCCTCC TGTGGTGGGT GGCATAGAAA AGAAATTTAC AGCCATAGCA 
CATTCTGATT GTACCTCAGA CACAGGAAGT AATGTGAAAA ATGTAACCTC TTCAATCACA 
GAAAATACAG AAAAAGGGGA GACTGATGGA ATAGACTGCG AAGATGCAGA ATATCAAATG 
ATGGCTACTC AGTGTTATCT AGAGCCCCAG GAAAAAGAGA CTGATATACA AGATGAAGAT 
GATTGTGCTG AGGAAGCTAC ACAAGCATTT ATCTTCAGTT CCACATGGGC TGAGCCGGAA 
CCATTTAAAC GTCCAGCCGA TCCTATTGGA GTTTTGCAAA TCTCAGCAGT GACTGTTAGT 
TCATCAGAGG AAGAACCAGA TGAAAATGCC ATTGCGGAGA CTCAGCCATT CTGCTGTGAG 
ATAAAGCAGC CAGAGGAACA CAGTGACCAA GAGCCAGCCA AAAAAAGTTC TATTGCTGAG 
GAAAAGAACA ATGTAGCTTG CTCTCTGCCT CAAGAAGAAA TATCTCAGGA TGATACCCAG 
CCAGTGTCTC AGTACTTGAA TAAAACAGCT TCAAGTAACT TGGTGGCACC AAATTTAACT 
TGCACAACAA ATTTTCAACA AGAAAAATAT CTTGATGGAA TTAATGAAGA AAAGGTCAAA 
CAGGCTAATG AAAGTGTTCC AAACAGGGAG GAACAGCTTG ATGAAGGTGT CCAAGACATC 
GAAGAGGATG CCACCCAACC ATATGTTTTA AGTTTACCGA CAGTGGGTGA CATGGCTACA 
CAGCCTTGTA GCATGAGTGA GTCTGTCTCC GGGGATGGTG CTAGTCAGTA TTTAAATACA 
GTTGTTCCAG CTGTAGCTAG TGTTGCTTCT CTACCCTGTA ACCCAAGCAT GCAATTATCT 
GATGGCGAAC CCATGCAGCC TTCCAGTCTA AATGAGGCTG ATGCAACCCA ATGTCATGGA 
TTTAGTATGC CCCTTAAAGA AGGTGATACA GTGAGCATTT CAGAAGAGAA AGTATCAGTA 
AAGCAAGGTC CTTGCAGAGG TGAGAGTAGC ATGATATCTG AGCAGTTAGA CCCTTCTAAC 
AATGTTATGA GCAAGCACCC AGGTGAAGTT CTTGTGCATT CTGTTCCTCC ATTGGAGAAA 
GCAACAGAGC AGGTTGCAGA GCAGCCTCAA GCAGCCACCA GAATGACAGA AGAAGACAGA 
GAAGAGAATA AAGGAAAGAG GAGATCAACA GAAGGAGAAG ATAGCAAGGA TTCAGCAGAA 
GTTACAACTT CCGTTACTAG GGGTAAAAAG AGAACCTGGA AAAAGACTGC ATCTCCAGCA 
TCAGAGGAGA CAAAAGGCAA AAAAACAGCT AAAATGCAGG TCATGGAAAA AGCATTACCT 
GAAGCAAAAA TGGAAGAAGA ATGTAAAGAA GAGCAGAACA ATAAGCCAAA CACTAGCTCT 
ATCCTTGCAC GTTTAAAAAC AGGTAGAAAG GGAAAACAAG ATATCGAAGA GCCTTTAGAT 
TACAATGTAG AAGGCTATAA TGTGAGCGTG CAACAGGTAG AAGAGGATAT GAAAAGATTT 
ACTTCAAGAA GATCAGTTAG AAAACCAAAG GATGGAAATG ATCAGGAGAC TGGGTCAAGA 
AATTTACCAG AAATAACCAG GCATTCCAGT TTAGACCTTA GTGAGTCAGT AGTGGAAAAA 
AAAGTAGATA TGTCTTCTGA TGAACACATG AAGTATCAGA AAGATGATGA ATTCTTAACT 
GCTAATGGAA CAACTATAAA TGTAAAAAGA AAGGGTGCAA AGAGAAATGT AAAGGAAGAG 
GAGAACAAGT TTGTGGTAGA TAACATTCCT GGAAAGCGAC CAACAAGGAA TAACAGGCCA 
AATGTTACAG AGAAAAATAA TGAGGAAGCA GATGATAAAC AGAGGATTAC AAGGAGGAAT 
TCAAGGCAGC TGGAGATAGT AGAAATCAAA CCAGACAATA CAGAGATTTT AGATTCTAAA 
AGACAAAACC AGTCCAGAAA ATCACCAAAT ATAAAGAAAA ATTTAAAAGT GGATGAATCA 
AAAGAGACAG AAAAATGTGA GGGCAAAACT AGAGAGAACA CTCCGAAAAG GACATCAAGG 
AGGACAAAGA AAAAATGCAC AGAGGAACTG AACATGGAAG AATGGGAAAA GTCAAAAGAA 
GAAGCAGACA GTAAACAGAG GGTCACAAGA AGGAACTCCA GGCTATCAGA GTCAGTAGAA 
AGCATACCAG AGGATACAGA TATTGTAGAT TCTGAGCAAC AAGACCAGTC CAGAAAATCT 
CGTAATATAA AGAAGAATTT GAAAAGGGGC GAGGAAGAAA AACTTGAGGG CACCAACAGA 
GACAACACTG AAAAATGGAC CTCAAGGAGG ACAAGAAAGG AATGCAAAGA GAAACTGAAC 
ATGGAGGACC ATGAAAAGTC GAAAGTAGAA CCATCCATTG AACAGTCTGA ACAAGAACAT 
ACTGAGGCTG AAGGACAAGA TCAATCTAGA AAATCTAGGA AGACAAAGAA GGATTTGCCA 
CAACCTGTTA TCAATGAGGA AGAAACCCTT GTAGAAACCA TCAGGAAAGA CTCTGTAGGA 
CGAACATCAA GGAGAAGAAA GAAGAGTTGC ATAGAGGAAG ATCTGATGGA CAGTGAGGTT 
CTAGTTATCA GTAAACCTGA ACAAGAAGAT CCACCCACAA AAAATGTTCC TAGAAGTAAA 
AGGAAGAATT TGAAAGATGA ACCTAAGGAG GGAGATACTG AGCATAATGT ATTTGTATCA 
AAGAATGAAA ATCTAGATGA AATGCAGACC ACTCAAAGGA AAAGCAATTC AAAAGATGGA 
GATGTGCAAA ACGATAATAA AACACTGTGT GAAGTCGACT CCAAGTCTGG TAATTCAGAT 
ATAAGAACAA GGAAGAAAAA AAAAGTTGTC ACAGGGACTC AAAAAACCAC AGAGGTAGAA 
GGGGAAATGC TAGATGGTAG CATGACAATA GACTCGCACG AAGACGTTTC ACCAACTGGA 
ATGAGAAAAA GCAGGCGGAC AACAACCAAG GAGGAATTAC TCAAGTCTAC CACTCTGGCT 
CCAGTAAGGA AGAGAGGGCA ACAGCCAAAA ACTGGAGGAG ATGAGGTCAA GAGAAAAAAA 
GTGAATGAAG AGTTTGAGCA GGAGAAAACA GTGGGTAGAA GGGGCCAAGC ACGGCAACCA 
AAGCTGGAGG ATGAAAATAC TGACGGCAAC AGTTTTGGAA AGGACTCGGC CGTTCCTTTT 
CCATCACCCT CGGGGAACTC CCGTTTAAGG CCAAGCACAT CCTTTGAAAG CCCGACAGAA 
GGCCGGACAC CAAGACGTGC TGCTCGCAGC TTGACCACAA GCCCCTACAT GTCCCAACGG 
AGCATTGCAG CAAAGGTTCT ATTTACAGGT GTGGTCGACC CTGCAGGTGA GGAGACCATT 
CGTAATCTAG GAGGGGAAGT TGCAGAATCC ATATTTGACT GCACTCATCT TGTAACTGAC 
CGTATCAGAC GTACCGTCAA ATTTCTCTGT GCTCTTGCCA AGGGCATCCC TATTGTGACA 
CTAGACTGGC TTGACAAGTG CAAAAAGAGC AGATGTTTTT TGTCCCCTGC TCAATTTCTG 
GTGAACGATA AAGAACAGGA GAAAAGCTTC AACTTCGATT TGTCAGAATC ATTGCAAAAA 
GCCAAAAAGA AACTGCTGTT TGAGGGCTAT GAAATTCATG TCACCCCGAA TGTGAAACCT 
GAGCCTGAAC ACATGAAAGA TATTATACAG TGCAGTGGGG CAACTTTCCT TCCTAAAATG 
CCCAAAGTAT ACAAGGACAA ATGCATCATT GTATCATGTA AGGAAGATGC CACTCGCTGC 
AAATCTGCTC CCTCCACTAT CCCTGTTACT TCTGCGGAAT TTATATTAAC TGGGATCCTG 
CGTCAGGAAA TAAATCCACA TGCTTATCTG CTAAGTACTG GACCTGAGGA AGCTTTGCCT 
ACCCCTGCCA AACGGAGGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mdc1 ( XM_002939467.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939513.3 Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X3, mRNA.

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CloneID	OXa64005
Clone ID Related Accession (Same CDS sequence)	XM_002939467.5
Accession Version	XM_002939467.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of DNA damage checkpoint protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939467.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGATA CTCAGCGCCT GCAGTGGGAC GATGAAGAAC AAGATGTGTC TTTTGATAGA 
GATAAACCAG TAGGAAATCT GCATATGTTC AATGGAATCC ATGGTCCAGC ACAAGACTTC 
CCTATTTACC CTGGTACGAA TATCATAGGA CGTCATACAA ATTGTGATGT GACTCTTCCA 
GCTCAGTCTG TCTCTAAGAA ACATGCTATT ATAGAGGTTG AGGCTGACTG CCATACCCTC 
TGTGACCAAG GAAGTCTAAA CAAAACTCGC CGTGGCAAGG CAGCCATTGC TCCTCATGTG 
AGATATGCTC TGTCTGATGG AGACTTATTG TTATTTGCTG ATGTAGCATG TCGTTACTCA 
TTCACAAAAA AACTAGAGGA GGAGGAGGCA GAGAATGAGG AAAGTGAAGA TGATATTTTG 
GTTCCTGGAA CACAGGCTAC TTTGTCAATT GAAAAAACTC CTGGGGTAGC AATACGCAGA 
ATGGGGAGAG GAGCAGTGCT GGCAATGGAC TCTGGTGATG AGGAAGAAGA GGAATTAAAA 
GGGAAAGATC TGAATTATGA AGCTAAAGGT CTTAAAACAT CCGGGGAGGA ATGCAAGTCA 
CCTGCCAGTG CTTCCTTTTC CCACAACATG GATACTATTG TTCCTGAAAG TGATGAGGAG 
AACGACACAT CTGTGTCTGA ACCACGTTTC CCTTCACTCA ACCTCCGTTG TGATAGTGAT 
ACAGACACTC ATGACACTCC CATGAAGGCA GGCACAAGCT ACACCCCCTC CCAAGATTGT 
GTGCTTTCTC CTTCTTTCAA AGAACAAGTT GTGGGAGACC ACAGTGAAAT CACAAAATCT 
ACTTTGATTA GCAACCAAGA GATGTTTGCA GATGAGCAAG TAAACACTGA ATCCAAAGTC 
TCGAAGTGTA GTCCTGATAC AGCTGACAAA AATGATAGAG AGACAAAGGA AGAGAACAAT 
ACTCCTATAG TAAACACTAA TATTGCTGAG GAAGGTGGAA CAGGAGTAAC AGATGATCCA 
TTGATAGACG GTCTAAAAGT GGAAAACCAA AAAAAGGAAA ATGACTGTTA TGAAACCCTG 
CAAGTTGACA TCAAACAGCA AGAGAGTGTT AAAATTATCT TGGATAGTAA TCAGGAGGAA 
AACAGAACTT TGAAAGTAAA ATTGGTAAAC ACAGGATCTG CAGATATTAT TCTAGACAAA 
AATATAGATG GGGAAGAAGG TTGCGTCAAT ACTAAGGAAA TAGACAACCA GATCAGAGAA 
GGGGCTTTTC ATTTGGACAG TGACACAGAT GTAGAGGAGA GCGATACTTC AATCCCTGGA 
ATAAATGTAC CCCGAGTGGA GAATTCTGGG AATAACACAG ACAGTGATAC AGATACTGAA 
GAAGATAAAC CTAAAATTTT GCAACCTGCC ATACAACAGA ATGTCGAGGA AGGGTTTAAT 
TTGGACAGTG ACACGGATGT TGAGGGTAAT GACTCCAATT CTGTAAATAA AAGGTTGAAA 
AATACTGAAA AAGCAAAAAT GATCCAAGAT AGTGATACTG ATGCGGATGA TTCGATTATG 
TCAAAGGTTG AAGAGAGGAG GAACCCAGCA GGTACAAAGA AAGATTACAG TAGTACATCT 
CTAAACACAG AAGGTAAAAG GCAAGAAAAT TATCTATCCA CCCTTGACAG TGATACAGAT 
GTTGAAGACA TTGCTGATGG ATCTTCCCCA AGTGTTGTGA AGAAGCAGGA TAAGGCAGAA 
GCAGCTCATA CTAATGTAAC CGATACAACT GCTGGGGAGG AAACTTGCAC AGATGCTGGA 
ATAGAGAAGA TTGTGGTGTC TGCAGATAGA AATGAATGCC ACACAGGTGG AGTCACACAA 
GAACAGGAGG CTACATCTGA ATTTCACCTA GACAGTGATA CAGATATTGA GGAAGATGAT 
AATTTACTAG ATATGTCAAG TGCTAGTACT AGGGTAAAAG TCACAGAGGT TCACTCAGTT 
ATCCTTGAAA AACTAAGAAC CTCACCTGGA GAAAGAAAGG AAAAGAGGGC TATTGAAATT 
GATCTGGACA GCAATACAGA TGTGGATGAA GCTGATGAAA CAGAAGTTGA TAGGAAGGAA 
ACATTTCACA TGGACAGTGA TACAGATCTG GAGGAAAATG ACAGTGGCCC TCCTGTGGTG 
GGTGGCATAG AAAAGAAATT TACAGCCATA GCACATTCTG ATTGTACCTC AGACACAGGA 
AGTAATGTGA AAAATGTAAC CTCTTCAATC ACAGAAAATA CAGAAAAAGG GGAGACTGAT 
GGAATAGACT GCGAAGATGC AGAATATCAA ATGATGGCTA CTCAGTGTTA TCTAGAGCCC 
CAGGAAAAAG AGACTGATAT ACAAGATGAA GATGATTGTG CTGAGGAAGC TACACAAGCA 
TTTATCTTCA GTTCCACATG GGCTGAGCCG GAACCATTTA AACGTCCAGC CGATCCTATT 
GGAGTTTTGC AAATCTCAGC AGTGACTGTT AGTTCATCAG AGGAAGAACC AGATGAAAAT 
GCCATTGCGG AGACTCAGCC ATTCTGCTGT GAGATAAAGC AGCCAGAGGA ACACAGTGAC 
CAAGAGCCAG CCAAAAAAAG TTCTATTGCT GAGGAAAAGA ACAATGTAGC TTGCTCTCTG 
CCTCAAGAAG AAATATCTCA GGATGATACC CAGCCAGTGT CTCAGTACTT GAATAAAACA 
GCTTCAAGTA ACTTGGTGGC ACCAAATTTA ACTTGCACAA CAAATTTTCA ACAAGAAAAA 
TATCTTGATG GAATTAATGA AGAAAAGGTC AAACAGGCTA ATGAAAGTGT TCCAAACAGG 
GAGGAACAGC TTGATGAAGG TGTCCAAGAC ATCGAAGAGG ATGCCACCCA ACCATATGTT 
TTAAGTTTAC CGACAGTGGG TGACATGGCT ACACAGCCTT GTAGCATGAG TGAGTCTGTC 
TCCGGGGATG GTGCTAGTCA GTATTTAAAT ACAGTTGTTC CAGCTGTAGC TAGTGTTGCT 
TCTCTACCCT GTAACCCAAG CATGCAATTA TCTGATGGCG AACCCATGCA GCCTTCCAGT 
CTAAATGAGG CTGATGCAAC CCAATGTCAT GGATTTAGTA TGCCCCTTAA AGAAGGTGAT 
ACAGTGAGCA TTTCAGAAGA GAAAGTATCA GTAAAGCAAG GTCCTTGCAG AGGTGAGAGT 
AGCATGATAT CTGAGCAGTT AGACCCTTCT AACAATGTTA TGAGCAAGCA CCCAGGTGAA 
GTTCTTGTGC ATTCTGTTCC TCCATTGGAG AAAGCAACAG AGCAGGTTGC AGAGCAGCCT 
CAAGCAGCCA CCAGAATGAC AGAAGAAGAC AGAGAAGAGA ATAAAGGAAA GAGGAGATCA 
ACAGAAGGAG AAGATAGCAA GGATTCAGCA GAAGTTACAA CTTCCGTTAC TAGGGGTAAA 
AAGAGAACCT GGAAAAAGAC TGCATCTCCA GCATCAGAGG AGACAAAAGG CAAAAAAACA 
GCTAAAATGC AGGTCATGGA AAAAGCATTA CCTGAAGCAA AAATGGAAGA AGAATGTAAA 
GAAGAGCAGA ACAATAAGCC AAACACTAGC TCTATCCTTG CACGTTTAAA AACAGGTAGA 
AAGGGAAAAC AAGATATCGA AGAGCCTTTA GATTACAATG TAGAAGGCTA TAATGTGAGC 
GTGCAACAGG TAGAAGAGGA TATGAAAAGA TTTACTTCAA GAAGATCAGT TAGAAAACCA 
AAGGATGGAA ATGATCAGGA GACTGGGTCA AGAAATTTAC CAGAAATAAC CAGGCATTCC 
AGTTTAGACC TTAGTGAGTC AGTAGTGGAA AAAAAAGTAG ATATGTCTTC TGATGAACAC 
ATGAAGTATC AGAAAGATGA TGAATTCTTA ACTGCTAATG GAACAACTAT AAATGTAAAA 
AGAAAGGGTG CAAAGAGAAA TGTAAAGGAA GAGGAGAACA AGTTTGTGGT AGATAACATT 
CCTGGAAAGC GACCAACAAG GAATAACAGG CCAAATGTTA CAGAGAAAAA TAATGAGGAA 
GCAGATGATA AACAGAGGAT TACAAGGAGG AATTCAAGGC AGCTGGAGAT AGTAGAAATC 
AAACCAGACA ATACAGAGAT TTTAGATTCT AAAAGACAAA ACCAGTCCAG AAAATCACCA 
AATATAAAGA AAAATTTAAA AGTGGATGAA TCAAAAGAGA CAGAAAAATG TGAGGGCAAA 
ACTAGAGAGA ACACTCCGAA AAGGACATCA AGGAGGACAA AGAAAAAATG CACAGAGGAA 
CTGAACATGG AAGAATGGGA AAAGTCAAAA GAAGAAGCAG ACAGTAAACA GAGGGTCACA 
AGAAGGAACT CCAGGCTATC AGAGTCAGTA GAAAGCATAC CAGAGGATAC AGATATTGTA 
GATTCTGAGC AACAAGACCA GTCCAGAAAA TCTCGTAATA TAAAGAAGAA TTTGAAAAGG 
GGCGAGGAAG AAAAACTTGA GGGCACCAAC AGAGACAACA CTGAAAAATG GACCTCAAGG 
AGGACAAGAA AGGAATGCAA AGAGAAACTG AACATGGAGG ACCATGAAAA GTCGAAAGTA 
GAACCATCCA TTGAACAGTC TGAACAAGAA CATACTGAGG CTGAAGGACA AGATCAATCT 
AGAAAATCTA GGAAGACAAA GAAGGATTTG CCACAACCTG TTATCAATGA GGAAGAAACC 
CTTGTAGAAA CCATCAGGAA AGACTCTGTA GGACGAACAT CAAGGAGAAG AAAGAAGAGT 
TGCATAGAGG AAGATCTGAT GGACAGTGAG GTTCTAGTTA TCAGTAAACC TGAACAAGAA 
GATCCACCCA CAAAAAATGT TCCTAGAAGT AAAAGGAAGA ATTTGAAAGA TGAACCTAAG 
GAGGGAGATA CTGAGCATAA TGTATTTGTA TCAAAGAATG AAAATCTAGA TGAAATGCAG 
ACCACTCAAA GGAAAAGCAA TTCAAAAGAT GGAGATGTGC AAAACGATAA TAAAACACTG 
TGTGAAGTCG ACTCCAAGTC TGGTAATTCA GATATAAGAA CAAGGAAGAA AAAAAAAGTT 
GTCACAGGGA CTCAAAAAAC CACAGAGGTA GAAGGGGAAA TGCTAGATGG TAGCATGACA 
ATAGACTCGC ACGAAGACGT TTCACCAACT GGAATGAGAA AAAGCAGGCG GACAACAACC 
AAGGAGGAAT TACTCAAGTC TACCACTCTG GCTCCAGTAA GGAAGAGAGG GCAACAGCCA 
AAAACTGGAG GAGATGAGGT CAAGAGAAAA AAAGTGAATG AAGAGTTTGA GCAGGAGAAA 
ACAGTGGGTA GAAGGGGCCA AGCACGGCAA CCAAAGCTGG AGGATGAAAA TACTGACGGC 
AACAGTTTTG GAAAGGACTC GGCCGTTCCT TTTCCATCAC CCTCGGGGAA CTCCCGTTTA 
AGGCCAAGCA CATCCTTTGA AAGCCCGACA GAAGGCCGGA CACCAAGACG TGCTGCTCGC 
AGCTTGACCA CAAGCCCCTA CATGTCCCAA CGGAGCATTG CAGCAAAGGT TCTATTTACA 
GGTGTGGTCG ACCCTGCAGG TGAGGAGACC ATTCGTAATC TAGGAGGGGA AGTTGCAGAA 
TCCATATTTG ACTGCACTCA TCTTGTAACT GACCGTATCA GACGTACCGT CAAATTTCTC 
TGTGCTCTTG CCAAGGGCAT CCCTATTGTG ACACTAGACT GGCTTGACAA GTGCAAAAAG 
AGCAGATGTT TTTTGTCCCC TGCTCAATTT CTGGTGAACG ATAAAGAACA GGAGAAAAGC 
TTCAACTTCG ATTTGTCAGA ATCATTGCAA AAAGCCAAAA AGAAACTGCT GTTTGAGGGC 
TATGAAATTC ATGTCACCCC GAATGTGAAA CCTGAGCCTG AACACATGAA AGATATTATA 
CAGTGCAGTG GGGCAACTTT CCTTCCTAAA ATGCCCAAAG TATACAAGGA CAAATGCATC 
ATTGTATCAT GTAAGGAAGA TGCCACTCGC TGCAAATCTG CTCCCTCCAC TATCCCTGTT 
ACTTCTGCGG AATTTATATT AACTGGGATC CTGCGTCAGG AAATAAATCC ACATGCTTAT 
CTGCTAAGTA CTGGACCTGA GGAAGCTTTG CCTACCCCTG CCAAACGGAG GCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939513.3
CDS	232..6348
Translation	MDDTQRLQWDDEEQDVSFDRDKPVGNLHMFNGIHGPAQDFPIYPGTNIIGRHTNCDVTLPAQSVSKKHAIIEVEADCHTLCDQGSLNKTRRGKAAIAPHVRYALSDGDLLLFADVACRYSFTKKLEEEEAENEESEDDILVPGTQATLSIEKTPGVAIRRMGRGAVLAMDSGDEEEEELKGKDLNYEAKGLKTSGEECKSPASASFSHNMDTIVPESDEENDTSVSEPRFPSLNLRCDSDTDTHDTPMKAGTSYTPSQDCVLSPSFKEQVVGDHSEITKSTLISNQEMFADEQVNTESKVSKCSPDTADKNDRETKEENNTPIVNTNIAEEGGTGVTDDPLIDGLKVENQKKENDCYETLQVDIKQQESVKIILDSNQEENRTLKVKLVNTGSADIILDKNIDGEEGCVNTKEIDNQIREGAFHLDSDTDVEESDTSIPGINVPRVENSGNNTDSDTDTEEDKPKILQPAIQQNVEEGFNLDSDTDVEGNDSNSVNKRLKNTEKAKMIQDSDTDADDSIMSKVEERRNPAGTKKDYSSTSLNTEGKRQENYLSTLDSDTDVEDIADGSSPSVVKKQDKAEAAHTNVTDTTAGEETCTDAGIEKIVVSADRNECHTGGVTQEQEATSEFHLDSDTDIEEDDNLLDMSSASTRVKVTEVHSVILEKLRTSPGERKEKRAIEIDLDSNTDVDEADETEVDRKETFHMDSDTDLEENDSGPPVVGGIEKKFTAIAHSDCTSDTGSNVKNVTSSITENTEKGETDGIDCEDAEYQMMATQCYLEPQEKETDIQDEDDCAEEATQAFIFSSTWAEPEPFKRPADPIGVLQISAVTVSSSEEEPDENAIAETQPFCCEIKQPEEHSDQEPAKKSSIAEEKNNVACSLPQEEISQDDTQPVSQYLNKTASSNLVAPNLTCTTNFQQEKYLDGINEEKVKQANESVPNREEQLDEGVQDIEEDATQPYVLSLPTVGDMATQPCSMSESVSGDGASQYLNTVVPAVASVASLPCNPSMQLSDGEPMQPSSLNEADATQCHGFSMPLKEGDTVSISEEKVSVKQGPCRGESSMISEQLDPSNNVMSKHPGEVLVHSVPPLEKATEQVAEQPQAATRMTEEDREENKGKRRSTEGEDSKDSAEVTTSVTRGKKRTWKKTASPASEETKGKKTAKMQVMEKALPEAKMEEECKEEQNNKPNTSSILARLKTGRKGKQDIEEPLDYNVEGYNVSVQQVEEDMKRFTSRRSVRKPKDGNDQETGSRNLPEITRHSSLDLSESVVEKKVDMSSDEHMKYQKDDEFLTANGTTINVKRKGAKRNVKEEENKFVVDNIPGKRPTRNNRPNVTEKNNEEADDKQRITRRNSRQLEIVEIKPDNTEILDSKRQNQSRKSPNIKKNLKVDESKETEKCEGKTRENTPKRTSRRTKKKCTEELNMEEWEKSKEEADSKQRVTRRNSRLSESVESIPEDTDIVDSEQQDQSRKSRNIKKNLKRGEEEKLEGTNRDNTEKWTSRRTRKECKEKLNMEDHEKSKVEPSIEQSEQEHTEAEGQDQSRKSRKTKKDLPQPVINEEETLVETIRKDSVGRTSRRRKKSCIEEDLMDSEVLVISKPEQEDPPTKNVPRSKRKNLKDEPKEGDTEHNVFVSKNENLDEMQTTQRKSNSKDGDVQNDNKTLCEVDSKSGNSDIRTRKKKKVVTGTQKTTEVEGEMLDGSMTIDSHEDVSPTGMRKSRRTTTKEELLKSTTLAPVRKRGQQPKTGGDEVKRKKVNEEFEQEKTVGRRGQARQPKLEDENTDGNSFGKDSAVPFPSPSGNSRLRPSTSFESPTEGRTPRRAARSLTTSPYMSQRSIAAKVLFTGVVDPAGEETIRNLGGEVAESIFDCTHLVTDRIRRTVKFLCALAKGIPIVTLDWLDKCKKSRCFLSPAQFLVNDKEQEKSFNFDLSESLQKAKKKLLFEGYEIHVTPNVKPEPEHMKDIIQCSGATFLPKMPKVYKDKCIIVSCKEDATRCKSAPSTIPVTSAEFILTGILRQEINPHAYLLSTGPEEALPTPAKRRR

Target ORF information:

RefSeq Version	XM_002939467.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator of DNA damage checkpoint 1 (mdc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939467.5

ORF Insert Sequence:

ATGGACGATA CTCAGCGCCT GCAGTGGGAC GATGAAGAAC AAGATGTGTC TTTTGATAGA 
GATAAACCAG TAGGAAATCT GCATATGTTC AATGGAATCC ATGGTCCAGC ACAAGACTTC 
CCTATTTACC CTGGTACGAA TATCATAGGA CGTCATACAA ATTGTGATGT GACTCTTCCA 
GCTCAGTCTG TCTCTAAGAA ACATGCTATT ATAGAGGTTG AGGCTGACTG CCATACCCTC 
TGTGACCAAG GAAGTCTAAA CAAAACTCGC CGTGGCAAGG CAGCCATTGC TCCTCATGTG 
AGATATGCTC TGTCTGATGG AGACTTATTG TTATTTGCTG ATGTAGCATG TCGTTACTCA 
TTCACAAAAA AACTAGAGGA GGAGGAGGCA GAGAATGAGG AAAGTGAAGA TGATATTTTG 
GTTCCTGGAA CACAGGCTAC TTTGTCAATT GAAAAAACTC CTGGGGTAGC AATACGCAGA 
ATGGGGAGAG GAGCAGTGCT GGCAATGGAC TCTGGTGATG AGGAAGAAGA GGAATTAAAA 
GGGAAAGATC TGAATTATGA AGCTAAAGGT CTTAAAACAT CCGGGGAGGA ATGCAAGTCA 
CCTGCCAGTG CTTCCTTTTC CCACAACATG GATACTATTG TTCCTGAAAG TGATGAGGAG 
AACGACACAT CTGTGTCTGA ACCACGTTTC CCTTCACTCA ACCTCCGTTG TGATAGTGAT 
ACAGACACTC ATGACACTCC CATGAAGGCA GGCACAAGCT ACACCCCCTC CCAAGATTGT 
GTGCTTTCTC CTTCTTTCAA AGAACAAGTT GTGGGAGACC ACAGTGAAAT CACAAAATCT 
ACTTTGATTA GCAACCAAGA GATGTTTGCA GATGAGCAAG TAAACACTGA ATCCAAAGTC 
TCGAAGTGTA GTCCTGATAC AGCTGACAAA AATGATAGAG AGACAAAGGA AGAGAACAAT 
ACTCCTATAG TAAACACTAA TATTGCTGAG GAAGGTGGAA CAGGAGTAAC AGATGATCCA 
TTGATAGACG GTCTAAAAGT GGAAAACCAA AAAAAGGAAA ATGACTGTTA TGAAACCCTG 
CAAGTTGACA TCAAACAGCA AGAGAGTGTT AAAATTATCT TGGATAGTAA TCAGGAGGAA 
AACAGAACTT TGAAAGTAAA ATTGGTAAAC ACAGGATCTG CAGATATTAT TCTAGACAAA 
AATATAGATG GGGAAGAAGG TTGCGTCAAT ACTAAGGAAA TAGACAACCA GATCAGAGAA 
GGGGCTTTTC ATTTGGACAG TGACACAGAT GTAGAGGAGA GCGATACTTC AATCCCTGGA 
ATAAATGTAC CCCGAGTGGA GAATTCTGGG AATAACACAG ACAGTGATAC AGATACTGAA 
GAAGATAAAC CTAAAATTTT GCAACCTGCC ATACAACAGA ATGTCGAGGA AGGGTTTAAT 
TTGGACAGTG ACACGGATGT TGAGGGTAAT GACTCCAATT CTGTAAATAA AAGGTTGAAA 
AATACTGAAA AAGCAAAAAT GATCCAAGAT AGTGATACTG ATGCGGATGA TTCGATTATG 
TCAAAGGTTG AAGAGAGGAG GAACCCAGCA GGTACAAAGA AAGATTACAG TAGTACATCT 
CTAAACACAG AAGGTAAAAG GCAAGAAAAT TATCTATCCA CCCTTGACAG TGATACAGAT 
GTTGAAGACA TTGCTGATGG ATCTTCCCCA AGTGTTGTGA AGAAGCAGGA TAAGGCAGAA 
GCAGCTCATA CTAATGTAAC CGATACAACT GCTGGGGAGG AAACTTGCAC AGATGCTGGA 
ATAGAGAAGA TTGTGGTGTC TGCAGATAGA AATGAATGCC ACACAGGTGG AGTCACACAA 
GAACAGGAGG CTACATCTGA ATTTCACCTA GACAGTGATA CAGATATTGA GGAAGATGAT 
AATTTACTAG ATATGTCAAG TGCTAGTACT AGGGTAAAAG TCACAGAGGT TCACTCAGTT 
ATCCTTGAAA AACTAAGAAC CTCACCTGGA GAAAGAAAGG AAAAGAGGGC TATTGAAATT 
GATCTGGACA GCAATACAGA TGTGGATGAA GCTGATGAAA CAGAAGTTGA TAGGAAGGAA 
ACATTTCACA TGGACAGTGA TACAGATCTG GAGGAAAATG ACAGTGGCCC TCCTGTGGTG 
GGTGGCATAG AAAAGAAATT TACAGCCATA GCACATTCTG ATTGTACCTC AGACACAGGA 
AGTAATGTGA AAAATGTAAC CTCTTCAATC ACAGAAAATA CAGAAAAAGG GGAGACTGAT 
GGAATAGACT GCGAAGATGC AGAATATCAA ATGATGGCTA CTCAGTGTTA TCTAGAGCCC 
CAGGAAAAAG AGACTGATAT ACAAGATGAA GATGATTGTG CTGAGGAAGC TACACAAGCA 
TTTATCTTCA GTTCCACATG GGCTGAGCCG GAACCATTTA AACGTCCAGC CGATCCTATT 
GGAGTTTTGC AAATCTCAGC AGTGACTGTT AGTTCATCAG AGGAAGAACC AGATGAAAAT 
GCCATTGCGG AGACTCAGCC ATTCTGCTGT GAGATAAAGC AGCCAGAGGA ACACAGTGAC 
CAAGAGCCAG CCAAAAAAAG TTCTATTGCT GAGGAAAAGA ACAATGTAGC TTGCTCTCTG 
CCTCAAGAAG AAATATCTCA GGATGATACC CAGCCAGTGT CTCAGTACTT GAATAAAACA 
GCTTCAAGTA ACTTGGTGGC ACCAAATTTA ACTTGCACAA CAAATTTTCA ACAAGAAAAA 
TATCTTGATG GAATTAATGA AGAAAAGGTC AAACAGGCTA ATGAAAGTGT TCCAAACAGG 
GAGGAACAGC TTGATGAAGG TGTCCAAGAC ATCGAAGAGG ATGCCACCCA ACCATATGTT 
TTAAGTTTAC CGACAGTGGG TGACATGGCT ACACAGCCTT GTAGCATGAG TGAGTCTGTC 
TCCGGGGATG GTGCTAGTCA GTATTTAAAT ACAGTTGTTC CAGCTGTAGC TAGTGTTGCT 
TCTCTACCCT GTAACCCAAG CATGCAATTA TCTGATGGCG AACCCATGCA GCCTTCCAGT 
CTAAATGAGG CTGATGCAAC CCAATGTCAT GGATTTAGTA TGCCCCTTAA AGAAGGTGAT 
ACAGTGAGCA TTTCAGAAGA GAAAGTATCA GTAAAGCAAG GTCCTTGCAG AGGTGAGAGT 
AGCATGATAT CTGAGCAGTT AGACCCTTCT AACAATGTTA TGAGCAAGCA CCCAGGTGAA 
GTTCTTGTGC ATTCTGTTCC TCCATTGGAG AAAGCAACAG AGCAGGTTGC AGAGCAGCCT 
CAAGCAGCCA CCAGAATGAC AGAAGAAGAC AGAGAAGAGA ATAAAGGAAA GAGGAGATCA 
ACAGAAGGAG AAGATAGCAA GGATTCAGCA GAAGTTACAA CTTCCGTTAC TAGGGGTAAA 
AAGAGAACCT GGAAAAAGAC TGCATCTCCA GCATCAGAGG AGACAAAAGG CAAAAAAACA 
GCTAAAATGC AGGTCATGGA AAAAGCATTA CCTGAAGCAA AAATGGAAGA AGAATGTAAA 
GAAGAGCAGA ACAATAAGCC AAACACTAGC TCTATCCTTG CACGTTTAAA AACAGGTAGA 
AAGGGAAAAC AAGATATCGA AGAGCCTTTA GATTACAATG TAGAAGGCTA TAATGTGAGC 
GTGCAACAGG TAGAAGAGGA TATGAAAAGA TTTACTTCAA GAAGATCAGT TAGAAAACCA 
AAGGATGGAA ATGATCAGGA GACTGGGTCA AGAAATTTAC CAGAAATAAC CAGGCATTCC 
AGTTTAGACC TTAGTGAGTC AGTAGTGGAA AAAAAAGTAG ATATGTCTTC TGATGAACAC 
ATGAAGTATC AGAAAGATGA TGAATTCTTA ACTGCTAATG GAACAACTAT AAATGTAAAA 
AGAAAGGGTG CAAAGAGAAA TGTAAAGGAA GAGGAGAACA AGTTTGTGGT AGATAACATT 
CCTGGAAAGC GACCAACAAG GAATAACAGG CCAAATGTTA CAGAGAAAAA TAATGAGGAA 
GCAGATGATA AACAGAGGAT TACAAGGAGG AATTCAAGGC AGCTGGAGAT AGTAGAAATC 
AAACCAGACA ATACAGAGAT TTTAGATTCT AAAAGACAAA ACCAGTCCAG AAAATCACCA 
AATATAAAGA AAAATTTAAA AGTGGATGAA TCAAAAGAGA CAGAAAAATG TGAGGGCAAA 
ACTAGAGAGA ACACTCCGAA AAGGACATCA AGGAGGACAA AGAAAAAATG CACAGAGGAA 
CTGAACATGG AAGAATGGGA AAAGTCAAAA GAAGAAGCAG ACAGTAAACA GAGGGTCACA 
AGAAGGAACT CCAGGCTATC AGAGTCAGTA GAAAGCATAC CAGAGGATAC AGATATTGTA 
GATTCTGAGC AACAAGACCA GTCCAGAAAA TCTCGTAATA TAAAGAAGAA TTTGAAAAGG 
GGCGAGGAAG AAAAACTTGA GGGCACCAAC AGAGACAACA CTGAAAAATG GACCTCAAGG 
AGGACAAGAA AGGAATGCAA AGAGAAACTG AACATGGAGG ACCATGAAAA GTCGAAAGTA 
GAACCATCCA TTGAACAGTC TGAACAAGAA CATACTGAGG CTGAAGGACA AGATCAATCT 
AGAAAATCTA GGAAGACAAA GAAGGATTTG CCACAACCTG TTATCAATGA GGAAGAAACC 
CTTGTAGAAA CCATCAGGAA AGACTCTGTA GGACGAACAT CAAGGAGAAG AAAGAAGAGT 
TGCATAGAGG AAGATCTGAT GGACAGTGAG GTTCTAGTTA TCAGTAAACC TGAACAAGAA 
GATCCACCCA CAAAAAATGT TCCTAGAAGT AAAAGGAAGA ATTTGAAAGA TGAACCTAAG 
GAGGGAGATA CTGAGCATAA TGTATTTGTA TCAAAGAATG AAAATCTAGA TGAAATGCAG 
ACCACTCAAA GGAAAAGCAA TTCAAAAGAT GGAGATGTGC AAAACGATAA TAAAACACTG 
TGTGAAGTCG ACTCCAAGTC TGGTAATTCA GATATAAGAA CAAGGAAGAA AAAAAAAGTT 
GTCACAGGGA CTCAAAAAAC CACAGAGGTA GAAGGGGAAA TGCTAGATGG TAGCATGACA 
ATAGACTCGC ACGAAGACGT TTCACCAACT GGAATGAGAA AAAGCAGGCG GACAACAACC 
AAGGAGGAAT TACTCAAGTC TACCACTCTG GCTCCAGTAA GGAAGAGAGG GCAACAGCCA 
AAAACTGGAG GAGATGAGGT CAAGAGAAAA AAAGTGAATG AAGAGTTTGA GCAGGAGAAA 
ACAGTGGGTA GAAGGGGCCA AGCACGGCAA CCAAAGCTGG AGGATGAAAA TACTGACGGC 
AACAGTTTTG GAAAGGACTC GGCCGTTCCT TTTCCATCAC CCTCGGGGAA CTCCCGTTTA 
AGGCCAAGCA CATCCTTTGA AAGCCCGACA GAAGGCCGGA CACCAAGACG TGCTGCTCGC 
AGCTTGACCA CAAGCCCCTA CATGTCCCAA CGGAGCATTG CAGCAAAGGT TCTATTTACA 
GGTGTGGTCG ACCCTGCAGG TGAGGAGACC ATTCGTAATC TAGGAGGGGA AGTTGCAGAA 
TCCATATTTG ACTGCACTCA TCTTGTAACT GACCGTATCA GACGTACCGT CAAATTTCTC 
TGTGCTCTTG CCAAGGGCAT CCCTATTGTG ACACTAGACT GGCTTGACAA GTGCAAAAAG 
AGCAGATGTT TTTTGTCCCC TGCTCAATTT CTGGTGAACG ATAAAGAACA GGAGAAAAGC 
TTCAACTTCG ATTTGTCAGA ATCATTGCAA AAAGCCAAAA AGAAACTGCT GTTTGAGGGC 
TATGAAATTC ATGTCACCCC GAATGTGAAA CCTGAGCCTG AACACATGAA AGATATTATA 
CAGTGCAGTG GGGCAACTTT CCTTCCTAAA ATGCCCAAAG TATACAAGGA CAAATGCATC 
ATTGTATCAT GTAAGGAAGA TGCCACTCGC TGCAAATCTG CTCCCTCCAC TATCCCTGTT 
ACTTCTGCGG AATTTATATT AACTGGGATC CTGCGTCAGG AAATAAATCC ACATGCTTAT 
CTGCTAAGTA CTGGACCTGA GGAAGCTTTG CCTACCCCTG CCAAACGGAG GCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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mdc1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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