ano4 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004912893.3	XP_004912950.1	anoctamin-4 isoform X1
XM_012959856.2	XP_012815310.1	anoctamin-4 isoform X2
XM_012959858.2	XP_012815312.1	anoctamin-4 isoform X3
XM_012959858.3	XP_012815312.2	anoctamin-4 isoform X3
XM_012959855.3	XP_012815309.2	anoctamin-4 isoform X1
XM_012959855.2	XP_012815309.1	anoctamin-4 isoform X1
XM_004912893.4	XP_004912950.2	anoctamin-4 isoform X1
XM_012959856.3	XP_012815310.2	anoctamin-4 isoform X2
XM_031898856.1	XP_031754716.1	anoctamin-4 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa07690	XM_012959858.2 Latest version!	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa54601	XM_012959858.3 Latest version!	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa54602	XM_031898856.1 Latest version!	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa54599	XM_012959855.3 Latest version!	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa54599	XM_004912893.4 Latest version!	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa54600	XM_012959856.3 Latest version!	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa07689	XM_004912893.3 Latest version!	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa07689	XM_012959855.2 Latest version!	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa34171	XM_012959856.2 Latest version!	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ano4 ( XM_012959858.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815312.1 Xenopus tropicalis anoctamin 4 (ano4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa07690
Clone ID Related Accession (Same CDS sequence)	XM_012959858.2
Accession Version	XM_012959858.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCT CCGGCAAATG TGCACTCTCA AATGGGAACA CAGAAATTCA GTACCCAGAA 
GTTAAAGATA TAAATATATT CTTTGATGAT TTAGAGGCTG CAAACAGTCC ATCAAAAGAT 
GATGATTCTT TGCTGCATGC TGGCAATTTA ACAAGCATGT CAGATGAAGT CAGCCGGTTG 
GACGCAGAAG GAGAGGTTTT GAGTGCTCCA GAAAAAAATA AAACAAGTGG CCTATTTTTT 
AATGATGGGA AGCGTCGTAT TGACTATATC CTGGTGTACA GAAAAACCAG TCTTCAGTCA 
GAAAAAAGGG AGATTTTTGA AAGAAATATT CGGGCGGAGG GATTGCATTT AGAGAAGGAG 
TCTTCCATTG TGAACAGTGA CATAATATTT GTAAAACTTC ATGCTCCATG GGAGGTACTA 
GGAAGATACG CAGAACTAAT GAATGTAAGG ATGCCTTTCA GGAGAAAAAT CTATTACCTG 
CACCGTCGGT ACAAGTTCAT GAGTAGGATG GAGAAGCAAT TGAGCAGGTT TCGAGGTTTG 
TTACCTAAAA AGCCCATGAG ACTGGACAAA GAGAAAATTC CAGACCTGGA GGAGAATGAC 
TGTTACACTG CGCCATTTAG TCAACAGAGG ATTCACCACT TCATAATAAA CAATAAAGAC 
ACATTTTTCA ACAATGCCAC AAGGACCAGA ATTGTACATC ATATTTTACA AAGAGTCAAA 
TATGAAGAGG GGAAAAATAA AATCGGACTG AACCGATTGC TCACAAACTG CACATATGAA 
GCTGCCTTCC CCCTACATGA GGGGAGTTAC AGAAGCAAAA ATTCAATAAA GACACATGGA 
GCAGAAAATC ACAGACATTT GCTTTTTGAA TGTTGGGCCT CCTGGGGAGT ATGGTATAAA 
TACCAACCAC TAGATCTGGT CAGGCGGTAT TTCGGAGAGA AGATAGGTCT GTATTTTGCA 
TGGCTTGGCT GGTACACAGG AATGCTTTTC CCAGCAGCCA TTGTTGGCTT GTTTGTTTTC 
CTCTATGGAA TGTTTACACT AGACAGCTGC CAAGTCAGTC GAGAAATCTG CCAAGCCACA 
AACATTACTA TGTGTCCACT ATGTGACAAA TATTGTCCAT ACATGAGGTT GTCAGACAGT 
TGCATCTATG CCAAGGTTAC CCATCTGTTT GACAACAGTG CAACAGTATT CTTTGCTGTT 
TTTATGGCAG TTTGGGCCAC AGTGTTTCTG GAGTTTTGGA AAAGAAGAAG AGCAGTAATT 
GCTTATGACT GGGACTTAAT AGACTGGGAA GAAGAAGAGG AAGAAATCCG CCCTCAGTTT 
GAAGCCAAAT ACTCAAAGAA AGAGCGTGTA AACCCAATAT CAGGAAAGCC AGAGCCTTAT 
CAAGCCTTTG CAGATAAATG CAGCAGACTT GTTGTTTCTG CTTCTGGAAT ATTTTTTATG 
ATATGTGTCG TAATTGCTGC AGTTTTCGGC ATTGTTATTT ATCGTGTTGT GACTGTCAGT 
ACGTTTGCTG CCTTTAAGTG GGCGTTAATA AGGAACAATT CTCAGGTTGC TACTACAGGG 
ACGGCGGTGT GCATAAACTT TTGCATTATA ATGCTTCTGA ATGTGCTCTA TGAAAGGGTT 
GCACTCTTCC TTACAAACCT AGAACAGCCT CGCACTGAAT CAGAATGGGA GAACAGCTTT 
ACTTTGAAAA TGTTTCTTTT TCAGTTTGTC AATCTGAACA GTTCCACCTT TTACATTGCA 
TTCTTTCTTG GGCGGTTTAC TGGCAGACCA GGGGCTTATT TAAGGCTCAT AAACAAATGG 
CGACTTGAAG AGTGTCACCC AAGTGGATGC CTTATTGACC TATGCCTGCA GATGGGAATA 
ATTATGGTTC TCAAACAGAC CTGGAATAAC TTCATGGAAC TTGGGTACCC ACTTATTCAG 
AACTGGTGGA CTCGAAGAAA ATTACGTCAA GAGCATGGAA TTCATGGAAA AACTGCCCTT 
CCACAGTGGG AAAAGGACTA CAACTTGCAG CCAATCAATC CATATGGACT CTTTGAAGAG 
TATCTGGAAA TGATTCTCCA GTTTGGCTTC ACAACCATCT TTGTGGCTGC CTTTCCATTA 
GCTCCTCTAC TTGCCCTACT TAACAACATC ATTGAAATCC GACTTGATGC ATATAAATTC 
ATCACTCAGT GGAGGCGGCC TCTAGCTTCC CGGGCAAAGG ATATAGGCAT ATGGTATGGA 
ATTCTTGAAG GCATCGGAAT TCTCTCAGTT ATCACCAACG CTTTTGTCAT AGCTGTGACC 
TCGGACTTCA TCCCTCGTCT GGTCTACGCT TACAAATATG GACCCTGTGC AGGTCAAGGA 
CGCGCTGGAG AAAAGTGCAT GGTCGGTTAT GTTAATGCCA GTCTTTCAGT ATTTTTGGTG 
ACTGATTTTG AAAACCATTC AAATTCTTCA CGCAATGGAA GAGAATTTGC TGAACAATAT 
GTAAAATATT GCAGGTACCG TGACTATAGA GACCCCCCAA GCGCTCCTGA GCCCTACGCT 
TATACACTGC AGTTCTGGCA TGTTTTAGCA GCTCGATTGG CATTCATTAT TGTATTTGAG 
CATCTTGTCT TTTTCATAAA GCATCTGATC TCTTACCTAA TACCAGACCT TCCCAAGGAC 
CTAAGGGACA GGATGAGAAG AGAAAAATAC CTTATACAGG AGATGATGTA TGAAGCAGAG 
CTAGAGAGAG TCCAAAAAGA AAAGAAAGAA AGAAAAAAAA ATGGAAAATG TCAACATAAT 
GAGTGGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815312.1
CDS	412..3183
Translation	MEASGKCALSNGNTEIQYPEVKDINIFFDDLEAANSPSKDDDSLLHAGNLTSMSDEVSRLDAEGEVLSAPEKNKTSGLFFNDGKRRIDYILVYRKTSLQSEKREIFERNIRAEGLHLEKESSIVNSDIIFVKLHAPWEVLGRYAELMNVRMPFRRKIYYLHRRYKFMSRMEKQLSRFRGLLPKKPMRLDKEKIPDLEENDCYTAPFSQQRIHHFIINNKDTFFNNATRTRIVHHILQRVKYEEGKNKIGLNRLLTNCTYEAAFPLHEGSYRSKNSIKTHGAENHRHLLFECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAIVGLFVFLYGMFTLDSCQVSREICQATNITMCPLCDKYCPYMRLSDSCIYAKVTHLFDNSATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERVNPISGKPEPYQAFADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYERVALFLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINKWRLEECHPSGCLIDLCLQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKLRQEHGIHGKTALPQWEKDYNLQPINPYGLFEEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFITQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCAGQGRAGEKCMVGYVNASLSVFLVTDFENHSNSSRNGREFAEQYVKYCRYRDYRDPPSAPEPYAYTLQFWHVLAARLAFIIVFEHLVFFIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERVQKEKKERKKNGKCQHNEWP

Target ORF information:

RefSeq Version	XM_012959858.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959858.2

ORF Insert Sequence:

ATGGAGGCCT CCGGCAAATG TGCACTCTCA AATGGGAACA CAGAAATTCA GTACCCAGAA 
GTTAAAGATA TAAATATATT CTTTGATGAT TTAGAGGCTG CAAACAGTCC ATCAAAAGAT 
GATGATTCTT TGCTGCATGC TGGCAATTTA ACAAGCATGT CAGATGAAGT CAGCCGGTTG 
GACGCAGAAG GAGAGGTTTT GAGTGCTCCA GAAAAAAATA AAACAAGTGG CCTATTTTTT 
AATGATGGGA AGCGTCGTAT TGACTATATC CTGGTGTACA GAAAAACCAG TCTTCAGTCA 
GAAAAAAGGG AGATTTTTGA AAGAAATATT CGGGCGGAGG GATTGCATTT AGAGAAGGAG 
TCTTCCATTG TGAACAGTGA CATAATATTT GTAAAACTTC ATGCTCCATG GGAGGTACTA 
GGAAGATACG CAGAACTAAT GAATGTAAGG ATGCCTTTCA GGAGAAAAAT CTATTACCTG 
CACCGTCGGT ACAAGTTCAT GAGTAGGATG GAGAAGCAAT TGAGCAGGTT TCGAGGTTTG 
TTACCTAAAA AGCCCATGAG ACTGGACAAA GAGAAAATTC CAGACCTGGA GGAGAATGAC 
TGTTACACTG CGCCATTTAG TCAACAGAGG ATTCACCACT TCATAATAAA CAATAAAGAC 
ACATTTTTCA ACAATGCCAC AAGGACCAGA ATTGTACATC ATATTTTACA AAGAGTCAAA 
TATGAAGAGG GGAAAAATAA AATCGGACTG AACCGATTGC TCACAAACTG CACATATGAA 
GCTGCCTTCC CCCTACATGA GGGGAGTTAC AGAAGCAAAA ATTCAATAAA GACACATGGA 
GCAGAAAATC ACAGACATTT GCTTTTTGAA TGTTGGGCCT CCTGGGGAGT ATGGTATAAA 
TACCAACCAC TAGATCTGGT CAGGCGGTAT TTCGGAGAGA AGATAGGTCT GTATTTTGCA 
TGGCTTGGCT GGTACACAGG AATGCTTTTC CCAGCAGCCA TTGTTGGCTT GTTTGTTTTC 
CTCTATGGAA TGTTTACACT AGACAGCTGC CAAGTCAGTC GAGAAATCTG CCAAGCCACA 
AACATTACTA TGTGTCCACT ATGTGACAAA TATTGTCCAT ACATGAGGTT GTCAGACAGT 
TGCATCTATG CCAAGGTTAC CCATCTGTTT GACAACAGTG CAACAGTATT CTTTGCTGTT 
TTTATGGCAG TTTGGGCCAC AGTGTTTCTG GAGTTTTGGA AAAGAAGAAG AGCAGTAATT 
GCTTATGACT GGGACTTAAT AGACTGGGAA GAAGAAGAGG AAGAAATCCG CCCTCAGTTT 
GAAGCCAAAT ACTCAAAGAA AGAGCGTGTA AACCCAATAT CAGGAAAGCC AGAGCCTTAT 
CAAGCCTTTG CAGATAAATG CAGCAGACTT GTTGTTTCTG CTTCTGGAAT ATTTTTTATG 
ATATGTGTCG TAATTGCTGC AGTTTTCGGC ATTGTTATTT ATCGTGTTGT GACTGTCAGT 
ACGTTTGCTG CCTTTAAGTG GGCGTTAATA AGGAACAATT CTCAGGTTGC TACTACAGGG 
ACGGCGGTGT GCATAAACTT TTGCATTATA ATGCTTCTGA ATGTGCTCTA TGAAAGGGTT 
GCACTCTTCC TTACAAACCT AGAACAGCCT CGCACTGAAT CAGAATGGGA GAACAGCTTT 
ACTTTGAAAA TGTTTCTTTT TCAGTTTGTC AATCTGAACA GTTCCACCTT TTACATTGCA 
TTCTTTCTTG GGCGGTTTAC TGGCAGACCA GGGGCTTATT TAAGGCTCAT AAACAAATGG 
CGACTTGAAG AGTGTCACCC AAGTGGATGC CTTATTGACC TATGCCTGCA GATGGGAATA 
ATTATGGTTC TCAAACAGAC CTGGAATAAC TTCATGGAAC TTGGGTACCC ACTTATTCAG 
AACTGGTGGA CTCGAAGAAA ATTACGTCAA GAGCATGGAA TTCATGGAAA AACTGCCCTT 
CCACAGTGGG AAAAGGACTA CAACTTGCAG CCAATCAATC CATATGGACT CTTTGAAGAG 
TATCTGGAAA TGATTCTCCA GTTTGGCTTC ACAACCATCT TTGTGGCTGC CTTTCCATTA 
GCTCCTCTAC TTGCCCTACT TAACAACATC ATTGAAATCC GACTTGATGC ATATAAATTC 
ATCACTCAGT GGAGGCGGCC TCTAGCTTCC CGGGCAAAGG ATATAGGCAT ATGGTATGGA 
ATTCTTGAAG GCATCGGAAT TCTCTCAGTT ATCACCAACG CTTTTGTCAT AGCTGTGACC 
TCGGACTTCA TCCCTCGTCT GGTCTACGCT TACAAATATG GACCCTGTGC AGGTCAAGGA 
CGCGCTGGAG AAAAGTGCAT GGTCGGTTAT GTTAATGCCA GTCTTTCAGT ATTTTTGGTG 
ACTGATTTTG AAAACCATTC AAATTCTTCA CGCAATGGAA GAGAATTTGC TGAACAATAT 
GTAAAATATT GCAGGTACCG TGACTATAGA GACCCCCCAA GCGCTCCTGA GCCCTACGCT 
TATACACTGC AGTTCTGGCA TGTTTTAGCA GCTCGATTGG CATTCATTAT TGTATTTGAG 
CATCTTGTCT TTTTCATAAA GCATCTGATC TCTTACCTAA TACCAGACCT TCCCAAGGAC 
CTAAGGGACA GGATGAGAAG AGAAAAATAC CTTATACAGG AGATGATGTA TGAAGCAGAG 
CTAGAGAGAG TCCAAAAAGA AAAGAAAGAA AGAAAAAAAA ATGGAAAATG TCAACATAAT 
GAGTGGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano4 ( XM_012959858.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815312.2 Xenopus tropicalis anoctamin 4 (ano4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa54601
Clone ID Related Accession (Same CDS sequence)	XM_012959858.3
Accession Version	XM_012959858.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959858.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCT CCGGCAAATG TGCACTCTCA AATGGGAACA CAGAAATTCA GTACCCAGGA 
GTTAAAGATA TAAATATATT CTTTGATGAT TTAGAGGCTG CAAACAGTCC ATCAAAAGAT 
GATGATTCTT TGCTGCATGC TGGCAATTTA ACAAGCATGT CAGATGAAGT CAGCCGGTTG 
GACGCAGAAG GAGAGGTTTT GAGTGCTCCA GAAAAAAATA AAACAAGTGG CCTATTTTTT 
AATGATGGGA AGCGTCGTAT TGACTATATC CTGGTGTACA GAAAAACCAG TCTTCAGTCA 
GAAAAAAGGG AGATTTTTGA AAGAAATATT CGGGCGGAGG GATTGCATTT AGAGAAGGAG 
TCTTCCATTG TGAACAGTGA CATAATATTT GTAAAACTTC ATGCTCCATG GGAGGTACTA 
GGAAGATACG CAGAACTAAT GAATGTAAGG ATGCCTTTCA GGAGAAAAAT CTATTACCTG 
CACCGTCGGT ACAAGTTCAT GAGTAGGATG GAGAAGCAAT TGAGCAGGTT TCGAGGTTTG 
TTACCTAAAA AGCCCATGAG ACTGGACAAA GAGAAAATTC CAGACCTGGA GGAGAATGAC 
TGTTACACTG CGCCATTTAG TCAACAGAGG ATTCACCACT TCATAATAAA CAATAAAGAC 
ACATTTTTCA ACAATGCCAC AAGGACCAGA ATTGTACATC ATATTTTACA AAGAGTCAAA 
TATGAAGAGG GGAAAAATAA AATCGGACTG AACCGATTGC TCACAAACTG CACATATGAA 
GCTGCCTTCC CCCTACATGA GGGGAGTTAC AGAAGCAAAA ATTCAATAAA GACACATGGA 
GCAGAAAATC ACAGACATTT GCTTTTTGAA TGTTGGGCCT CCTGGGGAGT ATGGTATAAA 
TACCAACCAC TAGATCTGGT CAGGCGGTAT TTCGGAGAGA AGATAGGTCT GTATTTTGCA 
TGGCTTGGCT GGTACACAGG AATGCTTTTC CCAGCAGCCA TTGTTGGCTT GTTTGTTTTC 
CTCTATGGAA TGTTTACACT AGACAGCTGC CAAGTCAGTC GAGAAATCTG CCAAGCCACA 
AACATTACTA TGTGTCCACT ATGTGACAAA TATTGTCCAT ACATGAGGTT GTCAGACAGT 
TGCATCTATG CCAAGGTTAC CCATCTGTTT GACAACAGTG CAACAGTATT CTTTGCTGTT 
TTTATGGCAG TTTGGGCCAC AGTGTTTCTG GAGTTTTGGA AAAGAAGAAG AGCAGTAATT 
GCTTATGACT GGGACTTAAT AGACTGGGAA GAAGAAGAGG AAGAAATCCG CCCTCAGTTT 
GAAGCCAAAT ACTCAAAGAA AGAGCGTGTA AACCCAATAT CAGGAAAGCC AGAGCCTTAT 
CAAGCCTTTG CAGATAAATG CAGCAGACTT GTTGTTTCTG CTTCTGGAAT ATTTTTTATG 
ATATGTGTCG TAATTGCTGC AGTTTTCGGC ATTGTTATTT ATCGTGTTGT GACTGTCAGT 
ACGTTTGCTG CCTTTAAGTG GGCGTTAATA AGGAACAATT CTCAGGTTGC TACTACAGGG 
ACGGCGGTGT GCATAAACTT TTGCATTATA ATGCTTCTGA ATGTGCTCTA TGAAAGGGTT 
GCACTCTTCC TTACAAACCT AGAACAGCCT CGCACTGAAT CAGAATGGGA GAACAGCTTT 
ACTTTGAAAA TGTTTCTTTT TCAGTTTGTC AATCTGAACA GTTCCACCTT TTACATTGCA 
TTCTTTCTTG GGCGGTTTAC TGGCAGACCA GGGGCTTATT TAAGGCTCAT AAACAAATGG 
CGACTTGAAG AGTGTCACCC AAGTGGATGC CTTATTGACC TATGCCTGCA GATGGGAATA 
ATTATGGTTC TCAAACAGAC CTGGAATAAC TTCATGGAAC TTGGGTACCC ACTTATTCAG 
AACTGGTGGA CTCGAAGAAA ATTACGTCAA GAGCATGGAA TTCATGGAAA AACTGCCCTT 
CCACAGTGGG AAAAGGACTA CAACTTGCAG CCAATCAATC CATATGGACT CTTTGAAGAG 
TATCTGGAAA TGATTCTCCA GTTTGGCTTC ACAACCATCT TTGTGGCTGC CTTTCCATTA 
GCTCCTCTAC TTGCCCTACT TAACAACATC ATTGAAATCC GACTTGATGC ATATAAATTC 
ATCACTCAGT GGAGGCGGCC TCTAGCTTCC CGGGCAAAGG ATATAGGCAT ATGGTATGGA 
ATTCTTGAAG GCATCGGAAT TCTCTCAGTT ATCACCAACG CTTTTGTCAT AGCTGTGACC 
TCGGACTTCA TCCCTCGTCT GGTCTACGCT TACAAATATG GACCCTGTGC AGGTCAAGGA 
CGCGCTGGAG AAAAGTGCAT GGTCGGTTAT GTTAATGCCA GTCTTTCAGT ATTTTTGGTG 
ACTGATTTTG AAAACCATTC AAATTCTTCA CGCAATGGAA GAGAATTTGC TGAACAATAT 
GTAAAATATT GCAGGTACCG TGACTATAGA GACCCCCCAA GCGCTCCTGA GCCCTACGCT 
TATACACTGC AGTTCTGGCA TGTTTTAGCA GCTCGATTGG CATTCATTAT TGTATTTGAG 
CATCTTGTCT TTTTCATAAA GCATCTGATC TCTTACCTAA TACCAGACCT TCCCAAGGAC 
CTAAGGGACA GGATGAGAAG AGAAAAATAC CTTATACAGG AGATGATGTA TGAAGCAGAG 
CTAGAGAGAG TCCAAAAAGA AAAGAAAGAA AGAAAAAAAA ATGGAAAATG TCAACATAAT 
GAGTGGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815312.2
CDS	564..3335
Translation	MEASGKCALSNGNTEIQYPGVKDINIFFDDLEAANSPSKDDDSLLHAGNLTSMSDEVSRLDAEGEVLSAPEKNKTSGLFFNDGKRRIDYILVYRKTSLQSEKREIFERNIRAEGLHLEKESSIVNSDIIFVKLHAPWEVLGRYAELMNVRMPFRRKIYYLHRRYKFMSRMEKQLSRFRGLLPKKPMRLDKEKIPDLEENDCYTAPFSQQRIHHFIINNKDTFFNNATRTRIVHHILQRVKYEEGKNKIGLNRLLTNCTYEAAFPLHEGSYRSKNSIKTHGAENHRHLLFECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAIVGLFVFLYGMFTLDSCQVSREICQATNITMCPLCDKYCPYMRLSDSCIYAKVTHLFDNSATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERVNPISGKPEPYQAFADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYERVALFLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINKWRLEECHPSGCLIDLCLQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKLRQEHGIHGKTALPQWEKDYNLQPINPYGLFEEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFITQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCAGQGRAGEKCMVGYVNASLSVFLVTDFENHSNSSRNGREFAEQYVKYCRYRDYRDPPSAPEPYAYTLQFWHVLAARLAFIIVFEHLVFFIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERVQKEKKERKKNGKCQHNEWP

Target ORF information:

RefSeq Version	XM_012959858.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959858.3

ORF Insert Sequence:

ATGGAGGCCT CCGGCAAATG TGCACTCTCA AATGGGAACA CAGAAATTCA GTACCCAGGA 
GTTAAAGATA TAAATATATT CTTTGATGAT TTAGAGGCTG CAAACAGTCC ATCAAAAGAT 
GATGATTCTT TGCTGCATGC TGGCAATTTA ACAAGCATGT CAGATGAAGT CAGCCGGTTG 
GACGCAGAAG GAGAGGTTTT GAGTGCTCCA GAAAAAAATA AAACAAGTGG CCTATTTTTT 
AATGATGGGA AGCGTCGTAT TGACTATATC CTGGTGTACA GAAAAACCAG TCTTCAGTCA 
GAAAAAAGGG AGATTTTTGA AAGAAATATT CGGGCGGAGG GATTGCATTT AGAGAAGGAG 
TCTTCCATTG TGAACAGTGA CATAATATTT GTAAAACTTC ATGCTCCATG GGAGGTACTA 
GGAAGATACG CAGAACTAAT GAATGTAAGG ATGCCTTTCA GGAGAAAAAT CTATTACCTG 
CACCGTCGGT ACAAGTTCAT GAGTAGGATG GAGAAGCAAT TGAGCAGGTT TCGAGGTTTG 
TTACCTAAAA AGCCCATGAG ACTGGACAAA GAGAAAATTC CAGACCTGGA GGAGAATGAC 
TGTTACACTG CGCCATTTAG TCAACAGAGG ATTCACCACT TCATAATAAA CAATAAAGAC 
ACATTTTTCA ACAATGCCAC AAGGACCAGA ATTGTACATC ATATTTTACA AAGAGTCAAA 
TATGAAGAGG GGAAAAATAA AATCGGACTG AACCGATTGC TCACAAACTG CACATATGAA 
GCTGCCTTCC CCCTACATGA GGGGAGTTAC AGAAGCAAAA ATTCAATAAA GACACATGGA 
GCAGAAAATC ACAGACATTT GCTTTTTGAA TGTTGGGCCT CCTGGGGAGT ATGGTATAAA 
TACCAACCAC TAGATCTGGT CAGGCGGTAT TTCGGAGAGA AGATAGGTCT GTATTTTGCA 
TGGCTTGGCT GGTACACAGG AATGCTTTTC CCAGCAGCCA TTGTTGGCTT GTTTGTTTTC 
CTCTATGGAA TGTTTACACT AGACAGCTGC CAAGTCAGTC GAGAAATCTG CCAAGCCACA 
AACATTACTA TGTGTCCACT ATGTGACAAA TATTGTCCAT ACATGAGGTT GTCAGACAGT 
TGCATCTATG CCAAGGTTAC CCATCTGTTT GACAACAGTG CAACAGTATT CTTTGCTGTT 
TTTATGGCAG TTTGGGCCAC AGTGTTTCTG GAGTTTTGGA AAAGAAGAAG AGCAGTAATT 
GCTTATGACT GGGACTTAAT AGACTGGGAA GAAGAAGAGG AAGAAATCCG CCCTCAGTTT 
GAAGCCAAAT ACTCAAAGAA AGAGCGTGTA AACCCAATAT CAGGAAAGCC AGAGCCTTAT 
CAAGCCTTTG CAGATAAATG CAGCAGACTT GTTGTTTCTG CTTCTGGAAT ATTTTTTATG 
ATATGTGTCG TAATTGCTGC AGTTTTCGGC ATTGTTATTT ATCGTGTTGT GACTGTCAGT 
ACGTTTGCTG CCTTTAAGTG GGCGTTAATA AGGAACAATT CTCAGGTTGC TACTACAGGG 
ACGGCGGTGT GCATAAACTT TTGCATTATA ATGCTTCTGA ATGTGCTCTA TGAAAGGGTT 
GCACTCTTCC TTACAAACCT AGAACAGCCT CGCACTGAAT CAGAATGGGA GAACAGCTTT 
ACTTTGAAAA TGTTTCTTTT TCAGTTTGTC AATCTGAACA GTTCCACCTT TTACATTGCA 
TTCTTTCTTG GGCGGTTTAC TGGCAGACCA GGGGCTTATT TAAGGCTCAT AAACAAATGG 
CGACTTGAAG AGTGTCACCC AAGTGGATGC CTTATTGACC TATGCCTGCA GATGGGAATA 
ATTATGGTTC TCAAACAGAC CTGGAATAAC TTCATGGAAC TTGGGTACCC ACTTATTCAG 
AACTGGTGGA CTCGAAGAAA ATTACGTCAA GAGCATGGAA TTCATGGAAA AACTGCCCTT 
CCACAGTGGG AAAAGGACTA CAACTTGCAG CCAATCAATC CATATGGACT CTTTGAAGAG 
TATCTGGAAA TGATTCTCCA GTTTGGCTTC ACAACCATCT TTGTGGCTGC CTTTCCATTA 
GCTCCTCTAC TTGCCCTACT TAACAACATC ATTGAAATCC GACTTGATGC ATATAAATTC 
ATCACTCAGT GGAGGCGGCC TCTAGCTTCC CGGGCAAAGG ATATAGGCAT ATGGTATGGA 
ATTCTTGAAG GCATCGGAAT TCTCTCAGTT ATCACCAACG CTTTTGTCAT AGCTGTGACC 
TCGGACTTCA TCCCTCGTCT GGTCTACGCT TACAAATATG GACCCTGTGC AGGTCAAGGA 
CGCGCTGGAG AAAAGTGCAT GGTCGGTTAT GTTAATGCCA GTCTTTCAGT ATTTTTGGTG 
ACTGATTTTG AAAACCATTC AAATTCTTCA CGCAATGGAA GAGAATTTGC TGAACAATAT 
GTAAAATATT GCAGGTACCG TGACTATAGA GACCCCCCAA GCGCTCCTGA GCCCTACGCT 
TATACACTGC AGTTCTGGCA TGTTTTAGCA GCTCGATTGG CATTCATTAT TGTATTTGAG 
CATCTTGTCT TTTTCATAAA GCATCTGATC TCTTACCTAA TACCAGACCT TCCCAAGGAC 
CTAAGGGACA GGATGAGAAG AGAAAAATAC CTTATACAGG AGATGATGTA TGAAGCAGAG 
CTAGAGAGAG TCCAAAAAGA AAAGAAAGAA AGAAAAAAAA ATGGAAAATG TCAACATAAT 
GAGTGGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano4 ( XM_031898856.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754716.1 Xenopus tropicalis anoctamin 4 (ano4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa54602
Clone ID Related Accession (Same CDS sequence)	XM_031898856.1
Accession Version	XM_031898856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTTC TTCTCGACTG GCTTCATTTT TGCTGTTCTT CCAGGAGAAA AATCTATTAC 
CTGCACCGTC GGTACAAGTT CATGAGTAGG ATGGAGAAGC AATTGAGCAG GTTTCGAGGT 
TTGTTACCTA AAAAGCCCAT GAGACTGGAC AAAGAGAAAA TTCCAGACCT GGAGGAGAAT 
GACTGTTACA CTGCGCCATT TAGTCAACAG AGGATTCACC ACTTCATAAT AAACAATAAA 
GACACATTTT TCAACAATGC CACAAGGACC AGAATTGTAC ATCATATTTT ACAAAGAGTC 
AAATATGAAG AGGGGAAAAA TAAAATCGGA CTGAACCGAT TGCTCACAAA CTGCACATAT 
GAAGCTGCCT TCCCCCTACA TGAGGGGAGT TACAGAAGCA AAAATTCAAT AAAGACACAT 
GGAGCAGAAA ATCACAGACA TTTGCTTTTT GAATGTTGGG CCTCCTGGGG AGTATGGTAT 
AAATACCAAC CACTAGATCT GGTCAGGCGG TATTTCGGAG AGAAGATAGG TCTGTATTTT 
GCATGGCTTG GCTGGTACAC AGGAATGCTT TTCCCAGCAG CCATTGTTGG CTTGTTTGTT 
TTCCTCTATG GAATGTTTAC ACTAGACAGC TGCCAAGTCA GTCGAGAAAT CTGCCAAGCC 
ACAAACATTA CTATGTGTCC ACTATGTGAC AAATATTGTC CATACATGAG GTTGTCAGAC 
AGTTGCATCT ATGCCAAGGT TACCCATCTG TTTGACAACA GTGCAACAGT ATTCTTTGCT 
GTTTTTATGG CAGTTTGGGC CACAGTGTTT CTGGAGTTTT GGAAAAGAAG AAGAGCAGTA 
ATTGCTTATG ACTGGGACTT AATAGACTGG GAAGAAGAAG AGGAAGAAAT CCGCCCTCAG 
TTTGAAGCCA AATACTCAAA GAAAGAGCGT GTAAACCCAA TATCAGGAAA GCCAGAGCCT 
TATCAAGCCT TTGCAGATAA ATGCAGCAGA CTTGTTGTTT CTGCTTCTGG AATATTTTTT 
ATGATATGTG TCGTAATTGC TGCAGTTTTC GGCATTGTTA TTTATCGTGT TGTGACTGTC 
AGTACGTTTG CTGCCTTTAA GTGGGCGTTA ATAAGGAACA ATTCTCAGGT TGCTACTACA 
GGGACGGCGG TGTGCATAAA CTTTTGCATT ATAATGCTTC TGAATGTGCT CTATGAAAGG 
GTTGCACTCT TCCTTACAAA CCTAGAACAG CCTCGCACTG AATCAGAATG GGAGAACAGC 
TTTACTTTGA AAATGTTTCT TTTTCAGTTT GTCAATCTGA ACAGTTCCAC CTTTTACATT 
GCATTCTTTC TTGGGCGGTT TACTGGCAGA CCAGGGGCTT ATTTAAGGCT CATAAACAAA 
TGGCGACTTG AAGAGTGTCA CCCAAGTGGA TGCCTTATTG ACCTATGCCT GCAGATGGGA 
ATAATTATGG TTCTCAAACA GACCTGGAAT AACTTCATGG AACTTGGGTA CCCACTTATT 
CAGAACTGGT GGACTCGAAG AAAATTACGT CAAGAGCATG GAATTCATGG AAAAACTGCC 
CTTCCACAGT GGGAAAAGGA CTACAACTTG CAGCCAATCA ATCCATATGG ACTCTTTGAA 
GAGTATCTGG AAATGATTCT CCAGTTTGGC TTCACAACCA TCTTTGTGGC TGCCTTTCCA 
TTAGCTCCTC TACTTGCCCT ACTTAACAAC ATCATTGAAA TCCGACTTGA TGCATATAAA 
TTCATCACTC AGTGGAGGCG GCCTCTAGCT TCCCGGGCAA AGGATATAGG CATATGGTAT 
GGAATTCTTG AAGGCATCGG AATTCTCTCA GTTATCACCA ACGCTTTTGT CATAGCTGTG 
ACCTCGGACT TCATCCCTCG TCTGGTCTAC GCTTACAAAT ATGGACCCTG TGCAGGTCAA 
GGACGCGCTG GAGAAAAGTG CATGGTCGGT TATGTTAATG CCAGTCTTTC AGTATTTTTG 
GTGACTGATT TTGAAAACCA TTCAAATTCT TCACGCAATG GAAGAGAATT TGCTGAACAA 
TATGTAAAAT ATTGCAGGTA CCGTGACTAT AGAGACCCCC CAAGCGCTCC TGAGCCCTAC 
GCTTATACAC TGCAGTTCTG GCATGTTTTA GCAGCTCGAT TGGCATTCAT TATTGTATTT 
GAGCATCTTG TCTTTTTCAT AAAGCATCTG ATCTCTTACC TAATACCAGA CCTTCCCAAG 
GACCTAAGGG ACAGGATGAG AAGAGAAAAA TACCTTATAC AGGAGATGAT GTATGAAGCA 
GAGCTAGAGA GAGTCCAAAA AGAAAAGAAA GAAAGAAAAA AAAATGGAAA ATGTCAACAT 
AATGAGTGGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754716.1
CDS	109..2463
Translation	MGFLLDWLHFCCSSRRKIYYLHRRYKFMSRMEKQLSRFRGLLPKKPMRLDKEKIPDLEENDCYTAPFSQQRIHHFIINNKDTFFNNATRTRIVHHILQRVKYEEGKNKIGLNRLLTNCTYEAAFPLHEGSYRSKNSIKTHGAENHRHLLFECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAIVGLFVFLYGMFTLDSCQVSREICQATNITMCPLCDKYCPYMRLSDSCIYAKVTHLFDNSATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERVNPISGKPEPYQAFADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYERVALFLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINKWRLEECHPSGCLIDLCLQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKLRQEHGIHGKTALPQWEKDYNLQPINPYGLFEEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFITQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCAGQGRAGEKCMVGYVNASLSVFLVTDFENHSNSSRNGREFAEQYVKYCRYRDYRDPPSAPEPYAYTLQFWHVLAARLAFIIVFEHLVFFIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERVQKEKKERKKNGKCQHNEWP

Target ORF information:

RefSeq Version	XM_031898856.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898856.1

ORF Insert Sequence:

ATGGGTTTTC TTCTCGACTG GCTTCATTTT TGCTGTTCTT CCAGGAGAAA AATCTATTAC 
CTGCACCGTC GGTACAAGTT CATGAGTAGG ATGGAGAAGC AATTGAGCAG GTTTCGAGGT 
TTGTTACCTA AAAAGCCCAT GAGACTGGAC AAAGAGAAAA TTCCAGACCT GGAGGAGAAT 
GACTGTTACA CTGCGCCATT TAGTCAACAG AGGATTCACC ACTTCATAAT AAACAATAAA 
GACACATTTT TCAACAATGC CACAAGGACC AGAATTGTAC ATCATATTTT ACAAAGAGTC 
AAATATGAAG AGGGGAAAAA TAAAATCGGA CTGAACCGAT TGCTCACAAA CTGCACATAT 
GAAGCTGCCT TCCCCCTACA TGAGGGGAGT TACAGAAGCA AAAATTCAAT AAAGACACAT 
GGAGCAGAAA ATCACAGACA TTTGCTTTTT GAATGTTGGG CCTCCTGGGG AGTATGGTAT 
AAATACCAAC CACTAGATCT GGTCAGGCGG TATTTCGGAG AGAAGATAGG TCTGTATTTT 
GCATGGCTTG GCTGGTACAC AGGAATGCTT TTCCCAGCAG CCATTGTTGG CTTGTTTGTT 
TTCCTCTATG GAATGTTTAC ACTAGACAGC TGCCAAGTCA GTCGAGAAAT CTGCCAAGCC 
ACAAACATTA CTATGTGTCC ACTATGTGAC AAATATTGTC CATACATGAG GTTGTCAGAC 
AGTTGCATCT ATGCCAAGGT TACCCATCTG TTTGACAACA GTGCAACAGT ATTCTTTGCT 
GTTTTTATGG CAGTTTGGGC CACAGTGTTT CTGGAGTTTT GGAAAAGAAG AAGAGCAGTA 
ATTGCTTATG ACTGGGACTT AATAGACTGG GAAGAAGAAG AGGAAGAAAT CCGCCCTCAG 
TTTGAAGCCA AATACTCAAA GAAAGAGCGT GTAAACCCAA TATCAGGAAA GCCAGAGCCT 
TATCAAGCCT TTGCAGATAA ATGCAGCAGA CTTGTTGTTT CTGCTTCTGG AATATTTTTT 
ATGATATGTG TCGTAATTGC TGCAGTTTTC GGCATTGTTA TTTATCGTGT TGTGACTGTC 
AGTACGTTTG CTGCCTTTAA GTGGGCGTTA ATAAGGAACA ATTCTCAGGT TGCTACTACA 
GGGACGGCGG TGTGCATAAA CTTTTGCATT ATAATGCTTC TGAATGTGCT CTATGAAAGG 
GTTGCACTCT TCCTTACAAA CCTAGAACAG CCTCGCACTG AATCAGAATG GGAGAACAGC 
TTTACTTTGA AAATGTTTCT TTTTCAGTTT GTCAATCTGA ACAGTTCCAC CTTTTACATT 
GCATTCTTTC TTGGGCGGTT TACTGGCAGA CCAGGGGCTT ATTTAAGGCT CATAAACAAA 
TGGCGACTTG AAGAGTGTCA CCCAAGTGGA TGCCTTATTG ACCTATGCCT GCAGATGGGA 
ATAATTATGG TTCTCAAACA GACCTGGAAT AACTTCATGG AACTTGGGTA CCCACTTATT 
CAGAACTGGT GGACTCGAAG AAAATTACGT CAAGAGCATG GAATTCATGG AAAAACTGCC 
CTTCCACAGT GGGAAAAGGA CTACAACTTG CAGCCAATCA ATCCATATGG ACTCTTTGAA 
GAGTATCTGG AAATGATTCT CCAGTTTGGC TTCACAACCA TCTTTGTGGC TGCCTTTCCA 
TTAGCTCCTC TACTTGCCCT ACTTAACAAC ATCATTGAAA TCCGACTTGA TGCATATAAA 
TTCATCACTC AGTGGAGGCG GCCTCTAGCT TCCCGGGCAA AGGATATAGG CATATGGTAT 
GGAATTCTTG AAGGCATCGG AATTCTCTCA GTTATCACCA ACGCTTTTGT CATAGCTGTG 
ACCTCGGACT TCATCCCTCG TCTGGTCTAC GCTTACAAAT ATGGACCCTG TGCAGGTCAA 
GGACGCGCTG GAGAAAAGTG CATGGTCGGT TATGTTAATG CCAGTCTTTC AGTATTTTTG 
GTGACTGATT TTGAAAACCA TTCAAATTCT TCACGCAATG GAAGAGAATT TGCTGAACAA 
TATGTAAAAT ATTGCAGGTA CCGTGACTAT AGAGACCCCC CAAGCGCTCC TGAGCCCTAC 
GCTTATACAC TGCAGTTCTG GCATGTTTTA GCAGCTCGAT TGGCATTCAT TATTGTATTT 
GAGCATCTTG TCTTTTTCAT AAAGCATCTG ATCTCTTACC TAATACCAGA CCTTCCCAAG 
GACCTAAGGG ACAGGATGAG AAGAGAAAAA TACCTTATAC AGGAGATGAT GTATGAAGCA 
GAGCTAGAGA GAGTCCAAAA AGAAAAGAAA GAAAGAAAAA AAAATGGAAA ATGTCAACAT 
AATGAGTGGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano4 ( XM_012959855.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815309.2 Xenopus tropicalis anoctamin 4 (ano4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa54599
Clone ID Related Accession (Same CDS sequence)	XM_012959855.3 , XM_004912893.4
Accession Version	XM_012959855.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959855.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA 
AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC 
TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC 
CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA 
CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG 
CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA 
ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT 
CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC 
ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT 
TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC 
CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG 
CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA 
AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA 
AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA 
GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815309.2
CDS	269..3742
Translation	MHCPHKGKLSAKPRSPRFEFGGAALELRRYKSIDTKKGNAGGFNLGSPRLQAPISVPTSPASRACSSPRGSPLTGYDTDPASESYNNLSSGTSYYSCVSQITIGFEVNGPVSIGISHWKPPYVHNETYLPFDHSQHNAEIPANPVSCSLLAQMDLSASLSCPTSLSVIPAVSRSSSQDTLSQDSDINQGISISIHGSKNQLQIHQKGSVASHSLPNEQTVVGHSSLPPLRKAIKMEASGKCALSNGNTEIQYPGVKDINIFFDDLEAANSPSKDDDSLLHAGNLTSMSDEVSRLDAEGEVLSAPEKNKTSGLFFNDGKRRIDYILVYRKTSLQSEKREIFERNIRAEGLHLEKESSIVNSDIIFVKLHAPWEVLGRYAELMNVRMPFRRKIYYLHRRYKFMSRMEKQLSRFRGLLPKKPMRLDKEKIPDLEENDCYTAPFSQQRIHHFIINNKDTFFNNATRTRIVHHILQRVKYEEGKNKIGLNRLLTNCTYEAAFPLHEGSYRSKNSIKTHGAENHRHLLFECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAIVGLFVFLYGMFTLDSCQVSREICQATNITMCPLCDKYCPYMRLSDSCIYAKVTHLFDNSATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERVNPISGKPEPYQAFADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYERVALFLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINKWRLEECHPSGCLIDLCLQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKLRQEHGIHGKTALPQWEKDYNLQPINPYGLFEEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFITQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCAGQGRAGEKCMVGYVNASLSVFLVTDFENHSNSSRNGREFAEQYVKYCRYRDYRDPPSAPEPYAYTLQFWHVLAARLAFIIVFEHLVFFIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERVQKEKKERKKNGKCQHNEWP

Target ORF information:

RefSeq Version	XM_012959855.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959855.3

ORF Insert Sequence:

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA 
AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC 
TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC 
CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA 
CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG 
CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA 
ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT 
CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC 
ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT 
TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC 
CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG 
CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA 
AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA 
AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA 
GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano4 ( XM_004912893.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912950.2 Xenopus tropicalis anoctamin 4 (ano4), transcript variant X1, mRNA.

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CloneID	OXa54599
Clone ID Related Accession (Same CDS sequence)	XM_012959855.3 , XM_004912893.4
Accession Version	XM_004912893.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912893.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA 
AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC 
TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC 
CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA 
CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG 
CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA 
ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT 
CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC 
ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT 
TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC 
CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG 
CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA 
AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA 
AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA 
GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912950.2
CDS	269..3742
Translation	MHCPHKGKLSAKPRSPRFEFGGAALELRRYKSIDTKKGNAGGFNLGSPRLQAPISVPTSPASRACSSPRGSPLTGYDTDPASESYNNLSSGTSYYSCVSQITIGFEVNGPVSIGISHWKPPYVHNETYLPFDHSQHNAEIPANPVSCSLLAQMDLSASLSCPTSLSVIPAVSRSSSQDTLSQDSDINQGISISIHGSKNQLQIHQKGSVASHSLPNEQTVVGHSSLPPLRKAIKMEASGKCALSNGNTEIQYPGVKDINIFFDDLEAANSPSKDDDSLLHAGNLTSMSDEVSRLDAEGEVLSAPEKNKTSGLFFNDGKRRIDYILVYRKTSLQSEKREIFERNIRAEGLHLEKESSIVNSDIIFVKLHAPWEVLGRYAELMNVRMPFRRKIYYLHRRYKFMSRMEKQLSRFRGLLPKKPMRLDKEKIPDLEENDCYTAPFSQQRIHHFIINNKDTFFNNATRTRIVHHILQRVKYEEGKNKIGLNRLLTNCTYEAAFPLHEGSYRSKNSIKTHGAENHRHLLFECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAIVGLFVFLYGMFTLDSCQVSREICQATNITMCPLCDKYCPYMRLSDSCIYAKVTHLFDNSATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERVNPISGKPEPYQAFADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYERVALFLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINKWRLEECHPSGCLIDLCLQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKLRQEHGIHGKTALPQWEKDYNLQPINPYGLFEEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFITQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCAGQGRAGEKCMVGYVNASLSVFLVTDFENHSNSSRNGREFAEQYVKYCRYRDYRDPPSAPEPYAYTLQFWHVLAARLAFIIVFEHLVFFIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERVQKEKKERKKNGKCQHNEWP

Target ORF information:

RefSeq Version	XM_004912893.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912893.4

ORF Insert Sequence:

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA 
AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC 
TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC 
CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA 
CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG 
CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA 
ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT 
CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC 
ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT 
TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC 
CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG 
CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA 
AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA 
AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA 
GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano4 ( XM_012959856.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815310.2 Xenopus tropicalis anoctamin 4 (ano4), transcript variant X3, mRNA.

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CloneID	OXa54600
Clone ID Related Accession (Same CDS sequence)	XM_012959856.3
Accession Version	XM_012959856.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGACTT ATTCAGAACT GGTGGACTCG AAGAAAATTA 
CGTCAAGAGC ATGGAATTCA TGGAAAAACT GCCCTTCCAC AGTGGGAAAA GGACTACAAC 
TTGCAGCCAA TCAATCCATA TGGACTCTTT GAAGAGTATC TGGAAATGAT TCTCCAGTTT 
GGCTTCACAA CCATCTTTGT GGCTGCCTTT CCATTAGCTC CTCTACTTGC CCTACTTAAC 
AACATCATTG AAATCCGACT TGATGCATAT AAATTCATCA CTCAGTGGAG GCGGCCTCTA 
GCTTCCCGGG CAAAGGATAT AGGCATATGG TATGGAATTC TTGAAGGCAT CGGAATTCTC 
TCAGTTATCA CCAACGCTTT TGTCATAGCT GTGACCTCGG ACTTCATCCC TCGTCTGGTC 
TACGCTTACA AATATGGACC CTGTGCAGGT CAAGGACGCG CTGGAGAAAA GTGCATGGTC 
GGTTATGTTA ATGCCAGTCT TTCAGTATTT TTGGTGACTG ATTTTGAAAA CCATTCAAAT 
TCTTCACGCA ATGGAAGAGA ATTTGCTGAA CAATATGTAA AATATTGCAG GTACCGTGAC 
TATAGAGACC CCCCAAGCGC TCCTGAGCCC TACGCTTATA CACTGCAGTT CTGGCATGTT 
TTAGCAGCTC GATTGGCATT CATTATTGTA TTTGAGCATC TTGTCTTTTT CATAAAGCAT 
CTGATCTCTT ACCTAATACC AGACCTTCCC AAGGACCTAA GGGACAGGAT GAGAAGAGAA 
AAATACCTTA TACAGGAGAT GATGTATGAA GCAGAGCTAG AGAGAGTCCA AAAAGAAAAG 
AAAGAAAGAA AAAAAAATGG AAAATGTCAA CATAATGAGT GGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815310.2
CDS	269..3736
Translation	MHCPHKGKLSAKPRSPRFEFGGAALELRRYKSIDTKKGNAGGFNLGSPRLQAPISVPTSPASRACSSPRGSPLTGYDTDPASESYNNLSSGTSYYSCVSQITIGFEVNGPVSIGISHWKPPYVHNETYLPFDHSQHNAEIPANPVSCSLLAQMDLSASLSCPTSLSVIPAVSRSSSQDTLSQDSDINQGISISIHGSKNQLQIHQKGSVASHSLPNEQTVVGHSSLPPLRKAIKMEASGKCALSNGNTEIQYPGVKDINIFFDDLEAANSPSKDDDSLLHAGNLTSMSDEVSRLDAEGEVLSAPEKNKTSGLFFNDGKRRIDYILVYRKTSLQSEKREIFERNIRAEGLHLEKESSIVNSDIIFVKLHAPWEVLGRYAELMNVRMPFRRKIYYLHRRYKFMSRMEKQLSRFRGLLPKKPMRLDKEKIPDLEENDCYTAPFSQQRIHHFIINNKDTFFNNATRTRIVHHILQRVKYEEGKNKIGLNRLLTNCTYEAAFPLHEGSYRSKNSIKTHGAENHRHLLFECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAIVGLFVFLYGMFTLDSCQVSREICQATNITMCPLCDKYCPYMRLSDSCIYAKVTHLFDNSATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERVNPISGKPEPYQAFADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYERVALFLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINKWRLEECHPSGCLIDLCLQMGIIMVLKQTWNNFMELGLIQNWWTRRKLRQEHGIHGKTALPQWEKDYNLQPINPYGLFEEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFITQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCAGQGRAGEKCMVGYVNASLSVFLVTDFENHSNSSRNGREFAEQYVKYCRYRDYRDPPSAPEPYAYTLQFWHVLAARLAFIIVFEHLVFFIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERVQKEKKERKKNGKCQHNEWP

Target ORF information:

RefSeq Version	XM_012959856.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959856.3

ORF Insert Sequence:

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGACTT ATTCAGAACT GGTGGACTCG AAGAAAATTA 
CGTCAAGAGC ATGGAATTCA TGGAAAAACT GCCCTTCCAC AGTGGGAAAA GGACTACAAC 
TTGCAGCCAA TCAATCCATA TGGACTCTTT GAAGAGTATC TGGAAATGAT TCTCCAGTTT 
GGCTTCACAA CCATCTTTGT GGCTGCCTTT CCATTAGCTC CTCTACTTGC CCTACTTAAC 
AACATCATTG AAATCCGACT TGATGCATAT AAATTCATCA CTCAGTGGAG GCGGCCTCTA 
GCTTCCCGGG CAAAGGATAT AGGCATATGG TATGGAATTC TTGAAGGCAT CGGAATTCTC 
TCAGTTATCA CCAACGCTTT TGTCATAGCT GTGACCTCGG ACTTCATCCC TCGTCTGGTC 
TACGCTTACA AATATGGACC CTGTGCAGGT CAAGGACGCG CTGGAGAAAA GTGCATGGTC 
GGTTATGTTA ATGCCAGTCT TTCAGTATTT TTGGTGACTG ATTTTGAAAA CCATTCAAAT 
TCTTCACGCA ATGGAAGAGA ATTTGCTGAA CAATATGTAA AATATTGCAG GTACCGTGAC 
TATAGAGACC CCCCAAGCGC TCCTGAGCCC TACGCTTATA CACTGCAGTT CTGGCATGTT 
TTAGCAGCTC GATTGGCATT CATTATTGTA TTTGAGCATC TTGTCTTTTT CATAAAGCAT 
CTGATCTCTT ACCTAATACC AGACCTTCCC AAGGACCTAA GGGACAGGAT GAGAAGAGAA 
AAATACCTTA TACAGGAGAT GATGTATGAA GCAGAGCTAG AGAGAGTCCA AAAAGAAAAG 
AAAGAAAGAA AAAAAAATGG AAAATGTCAA CATAATGAGT GGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano4 ( XM_004912893.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912950.1 Xenopus tropicalis anoctamin 4 (ano4), transcript variant X1, mRNA.

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CloneID	OXa07689
Clone ID Related Accession (Same CDS sequence)	XM_004912893.3 , XM_012959855.2
Accession Version	XM_004912893.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA 
AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC 
TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC 
CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA 
CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG 
CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA 
ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT 
CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC 
ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT 
TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC 
CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG 
CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA 
AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA 
AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA 
GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912950.1
CDS	261..3734
Translation	MHCPHKGKLSAKPRSPRFEFGGAALELRRYKSIDTKKGNAGGFNLGSPRLQAPISVPTSPASRACSSPRGSPLTGYDTDPASESYNNLSSGTSYYSCVSQITIGFEVNGPVSIGISHWKPPYAHNETYLPFDHSQHNAEIPANPVSCSLLAQMDLSASLSCPTSLSVIPAVSRSSSQDTLSQDSDINQGISISIHGSKNQLQIHQKGSVASHSLPNEQTVVGHSSLPPLRKAIKMEASGKCALSNGNTEIQYPEVKDINIFFDDLEAANSPSKDDDSLLHAGNLTSMSDEVSRLDAEGEVLSAPEKNKTSGLFFNDGKRRIDYILVYRKTSLQSEKREIFERNIRAEGLHLEKESSIVNSDIIFVKLHAPWEVLGRYAELMNVRMPFRRKIYYLHRRYKFMSRMEKQLSRFRGLLPKKPMRLDKEKIPDLEENDCYTAPFSQQRIHHFIINNKDTFFNNATRTRIVHHILQRVKYEEGKNKIGLNRLLTNCTYEAAFPLHEGSYRSKNSIKTHGAENHRHLLFECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAIVGLFVFLYGMFTLDSCQVSREICQATNITMCPLCDKYCPYMRLSDSCIYAKVTHLFDNSATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERVNPISGKPEPYQAFADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYERVALFLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINKWRLEECHPSGCLIDLCLQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKLRQEHGIHGKTALPQWEKDYNLQPINPYGLFEEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFITQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCAGQGRAGEKCMVGYVNASLSVFLVTDFENHSNSSRNGREFAEQYVKYCRYRDYRDPPSAPEPYAYTLQFWHVLAARLAFIIVFEHLVFFIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERVQKEKKERKKNGKCQHNEWP

Target ORF information:

RefSeq Version	XM_004912893.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912893.3

ORF Insert Sequence:

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA 
AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC 
TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC 
CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA 
CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG 
CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA 
ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT 
CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC 
ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT 
TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC 
CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG 
CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA 
AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA 
AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA 
GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano4 ( XM_012959855.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815309.1 Xenopus tropicalis anoctamin 4 (ano4), transcript variant X2, mRNA.

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CloneID	OXa07689
Clone ID Related Accession (Same CDS sequence)	XM_004912893.3 , XM_012959855.2
Accession Version	XM_012959855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA 
AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC 
TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC 
CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA 
CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG 
CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA 
ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT 
CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC 
ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT 
TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC 
CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG 
CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA 
AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA 
AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA 
GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815309.1
CDS	261..3734
Translation	MHCPHKGKLSAKPRSPRFEFGGAALELRRYKSIDTKKGNAGGFNLGSPRLQAPISVPTSPASRACSSPRGSPLTGYDTDPASESYNNLSSGTSYYSCVSQITIGFEVNGPVSIGISHWKPPYAHNETYLPFDHSQHNAEIPANPVSCSLLAQMDLSASLSCPTSLSVIPAVSRSSSQDTLSQDSDINQGISISIHGSKNQLQIHQKGSVASHSLPNEQTVVGHSSLPPLRKAIKMEASGKCALSNGNTEIQYPEVKDINIFFDDLEAANSPSKDDDSLLHAGNLTSMSDEVSRLDAEGEVLSAPEKNKTSGLFFNDGKRRIDYILVYRKTSLQSEKREIFERNIRAEGLHLEKESSIVNSDIIFVKLHAPWEVLGRYAELMNVRMPFRRKIYYLHRRYKFMSRMEKQLSRFRGLLPKKPMRLDKEKIPDLEENDCYTAPFSQQRIHHFIINNKDTFFNNATRTRIVHHILQRVKYEEGKNKIGLNRLLTNCTYEAAFPLHEGSYRSKNSIKTHGAENHRHLLFECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAIVGLFVFLYGMFTLDSCQVSREICQATNITMCPLCDKYCPYMRLSDSCIYAKVTHLFDNSATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERVNPISGKPEPYQAFADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYERVALFLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINKWRLEECHPSGCLIDLCLQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKLRQEHGIHGKTALPQWEKDYNLQPINPYGLFEEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFITQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCAGQGRAGEKCMVGYVNASLSVFLVTDFENHSNSSRNGREFAEQYVKYCRYRDYRDPPSAPEPYAYTLQFWHVLAARLAFIIVFEHLVFFIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERVQKEKKERKKNGKCQHNEWP

Target ORF information:

RefSeq Version	XM_012959855.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959855.2

ORF Insert Sequence:

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA 
AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC 
TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC 
CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA 
CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG 
CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA 
ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT 
CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC 
ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT 
TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC 
CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG 
CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA 
AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA 
AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA 
GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano4 ( XM_012959856.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815310.1 Xenopus tropicalis anoctamin 4 (ano4), transcript variant X3, mRNA.

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CloneID	OXa34171
Clone ID Related Accession (Same CDS sequence)	XM_012959856.2
Accession Version	XM_012959856.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGACTT ATTCAGAACT GGTGGACTCG AAGAAAATTA 
CGTCAAGAGC ATGGAATTCA TGGAAAAACT GCCCTTCCAC AGTGGGAAAA GGACTACAAC 
TTGCAGCCAA TCAATCCATA TGGACTCTTT GAAGAGTATC TGGAAATGAT TCTCCAGTTT 
GGCTTCACAA CCATCTTTGT GGCTGCCTTT CCATTAGCTC CTCTACTTGC CCTACTTAAC 
AACATCATTG AAATCCGACT TGATGCATAT AAATTCATCA CTCAGTGGAG GCGGCCTCTA 
GCTTCCCGGG CAAAGGATAT AGGCATATGG TATGGAATTC TTGAAGGCAT CGGAATTCTC 
TCAGTTATCA CCAACGCTTT TGTCATAGCT GTGACCTCGG ACTTCATCCC TCGTCTGGTC 
TACGCTTACA AATATGGACC CTGTGCAGGT CAAGGACGCG CTGGAGAAAA GTGCATGGTC 
GGTTATGTTA ATGCCAGTCT TTCAGTATTT TTGGTGACTG ATTTTGAAAA CCATTCAAAT 
TCTTCACGCA ATGGAAGAGA ATTTGCTGAA CAATATGTAA AATATTGCAG GTACCGTGAC 
TATAGAGACC CCCCAAGCGC TCCTGAGCCC TACGCTTATA CACTGCAGTT CTGGCATGTT 
TTAGCAGCTC GATTGGCATT CATTATTGTA TTTGAGCATC TTGTCTTTTT CATAAAGCAT 
CTGATCTCTT ACCTAATACC AGACCTTCCC AAGGACCTAA GGGACAGGAT GAGAAGAGAA 
AAATACCTTA TACAGGAGAT GATGTATGAA GCAGAGCTAG AGAGAGTCCA AAAAGAAAAG 
AAAGAAAGAA AAAAAAATGG AAAATGTCAA CATAATGAGT GGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815310.1
CDS	261..3728
Translation	MHCPHKGKLSAKPRSPRFEFGGAALELRRYKSIDTKKGNAGGFNLGSPRLQAPISVPTSPASRACSSPRGSPLTGYDTDPASESYNNLSSGTSYYSCVSQITIGFEVNGPVSIGISHWKPPYAHNETYLPFDHSQHNAEIPANPVSCSLLAQMDLSASLSCPTSLSVIPAVSRSSSQDTLSQDSDINQGISISIHGSKNQLQIHQKGSVASHSLPNEQTVVGHSSLPPLRKAIKMEASGKCALSNGNTEIQYPEVKDINIFFDDLEAANSPSKDDDSLLHAGNLTSMSDEVSRLDAEGEVLSAPEKNKTSGLFFNDGKRRIDYILVYRKTSLQSEKREIFERNIRAEGLHLEKESSIVNSDIIFVKLHAPWEVLGRYAELMNVRMPFRRKIYYLHRRYKFMSRMEKQLSRFRGLLPKKPMRLDKEKIPDLEENDCYTAPFSQQRIHHFIINNKDTFFNNATRTRIVHHILQRVKYEEGKNKIGLNRLLTNCTYEAAFPLHEGSYRSKNSIKTHGAENHRHLLFECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAIVGLFVFLYGMFTLDSCQVSREICQATNITMCPLCDKYCPYMRLSDSCIYAKVTHLFDNSATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERVNPISGKPEPYQAFADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYERVALFLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINKWRLEECHPSGCLIDLCLQMGIIMVLKQTWNNFMELGLIQNWWTRRKLRQEHGIHGKTALPQWEKDYNLQPINPYGLFEEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFITQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCAGQGRAGEKCMVGYVNASLSVFLVTDFENHSNSSRNGREFAEQYVKYCRYRDYRDPPSAPEPYAYTLQFWHVLAARLAFIIVFEHLVFFIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERVQKEKKERKKNGKCQHNEWP

Target ORF information:

RefSeq Version	XM_012959856.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 4 (ano4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959856.2

ORF Insert Sequence:

ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC 
GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG 
GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT 
GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG 
ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT 
CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT 
CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT 
TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA 
AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG 
CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG 
GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA 
TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA 
TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT 
GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT 
TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT 
ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT 
GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT 
GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA 
ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC 
ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG 
AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT 
AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC 
ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT 
AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT 
GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT 
TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG 
GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA 
GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA 
CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA 
CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT 
ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC 
ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA 
ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG 
AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA 
TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT 
GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG 
TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC 
TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC 
CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT 
TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT 
ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC 
CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG 
ACCTGGAATA ACTTCATGGA ACTTGGACTT ATTCAGAACT GGTGGACTCG AAGAAAATTA 
CGTCAAGAGC ATGGAATTCA TGGAAAAACT GCCCTTCCAC AGTGGGAAAA GGACTACAAC 
TTGCAGCCAA TCAATCCATA TGGACTCTTT GAAGAGTATC TGGAAATGAT TCTCCAGTTT 
GGCTTCACAA CCATCTTTGT GGCTGCCTTT CCATTAGCTC CTCTACTTGC CCTACTTAAC 
AACATCATTG AAATCCGACT TGATGCATAT AAATTCATCA CTCAGTGGAG GCGGCCTCTA 
GCTTCCCGGG CAAAGGATAT AGGCATATGG TATGGAATTC TTGAAGGCAT CGGAATTCTC 
TCAGTTATCA CCAACGCTTT TGTCATAGCT GTGACCTCGG ACTTCATCCC TCGTCTGGTC 
TACGCTTACA AATATGGACC CTGTGCAGGT CAAGGACGCG CTGGAGAAAA GTGCATGGTC 
GGTTATGTTA ATGCCAGTCT TTCAGTATTT TTGGTGACTG ATTTTGAAAA CCATTCAAAT 
TCTTCACGCA ATGGAAGAGA ATTTGCTGAA CAATATGTAA AATATTGCAG GTACCGTGAC 
TATAGAGACC CCCCAAGCGC TCCTGAGCCC TACGCTTATA CACTGCAGTT CTGGCATGTT 
TTAGCAGCTC GATTGGCATT CATTATTGTA TTTGAGCATC TTGTCTTTTT CATAAAGCAT 
CTGATCTCTT ACCTAATACC AGACCTTCCC AAGGACCTAA GGGACAGGAT GAGAAGAGAA 
AAATACCTTA TACAGGAGAT GATGTATGAA GCAGAGCTAG AGAGAGTCCA AAAAGAAAAG 
AAAGAAAGAA AAAAAAATGG AAAATGTCAA CATAATGAGT GGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ano4
Entrez Gene ID	100488512
Full Name	anoctamin 4
Synonyms	tmem16d
General protein information	Preferred Names anoctamin 4 Names anoctamin-4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Canis lupus familiaris (dog)	ANO4	XP_003432088.1
Gallus gallus (chicken)	ANO4	XP_425452.4
Pan troglodytes (chimpanzee)	ANO4	XP_509302.2
Mus musculus (house mouse)	Ano4	NP_001264117.1
	ANO4	XP_001090523.1
Bos taurus (cattle)	ANO4	NP_001095520.1
Xenopus tropicalis (tropical clawed frog)	ano4	XP_002936887.2
Homo sapiens (human)	ANO4	NP_001273544.1
Rattus norvegicus (Norway rat)	Ano4	NP_001100248.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ano4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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