ano4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ano4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ano4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa07690 XM_012959858.2
Latest version!
Xenopus tropicalis anoctamin 4 (ano4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa54601 XM_012959858.3
Latest version!
Xenopus tropicalis anoctamin 4 (ano4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa54602 XM_031898856.1
Latest version!
Xenopus tropicalis anoctamin 4 (ano4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa54599 XM_012959855.3
Latest version!
Xenopus tropicalis anoctamin 4 (ano4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa54599 XM_004912893.4
Latest version!
Xenopus tropicalis anoctamin 4 (ano4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa54600 XM_012959856.3
Latest version!
Xenopus tropicalis anoctamin 4 (ano4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa07689 XM_004912893.3
Latest version!
Xenopus tropicalis anoctamin 4 (ano4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa07689 XM_012959855.2
Latest version!
Xenopus tropicalis anoctamin 4 (ano4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa34171 XM_012959856.2
Latest version!
Xenopus tropicalis anoctamin 4 (ano4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa07690
    Clone ID Related Accession (Same CDS sequence) XM_012959858.2
    Accession Version XM_012959858.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product anoctamin-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGGCCT CCGGCAAATG TGCACTCTCA AATGGGAACA CAGAAATTCA GTACCCAGAA 
    GTTAAAGATA TAAATATATT CTTTGATGAT TTAGAGGCTG CAAACAGTCC ATCAAAAGAT
    GATGATTCTT TGCTGCATGC TGGCAATTTA ACAAGCATGT CAGATGAAGT CAGCCGGTTG
    GACGCAGAAG GAGAGGTTTT GAGTGCTCCA GAAAAAAATA AAACAAGTGG CCTATTTTTT
    AATGATGGGA AGCGTCGTAT TGACTATATC CTGGTGTACA GAAAAACCAG TCTTCAGTCA
    GAAAAAAGGG AGATTTTTGA AAGAAATATT CGGGCGGAGG GATTGCATTT AGAGAAGGAG
    TCTTCCATTG TGAACAGTGA CATAATATTT GTAAAACTTC ATGCTCCATG GGAGGTACTA
    GGAAGATACG CAGAACTAAT GAATGTAAGG ATGCCTTTCA GGAGAAAAAT CTATTACCTG
    CACCGTCGGT ACAAGTTCAT GAGTAGGATG GAGAAGCAAT TGAGCAGGTT TCGAGGTTTG
    TTACCTAAAA AGCCCATGAG ACTGGACAAA GAGAAAATTC CAGACCTGGA GGAGAATGAC
    TGTTACACTG CGCCATTTAG TCAACAGAGG ATTCACCACT TCATAATAAA CAATAAAGAC
    ACATTTTTCA ACAATGCCAC AAGGACCAGA ATTGTACATC ATATTTTACA AAGAGTCAAA
    TATGAAGAGG GGAAAAATAA AATCGGACTG AACCGATTGC TCACAAACTG CACATATGAA
    GCTGCCTTCC CCCTACATGA GGGGAGTTAC AGAAGCAAAA ATTCAATAAA GACACATGGA
    GCAGAAAATC ACAGACATTT GCTTTTTGAA TGTTGGGCCT CCTGGGGAGT ATGGTATAAA
    TACCAACCAC TAGATCTGGT CAGGCGGTAT TTCGGAGAGA AGATAGGTCT GTATTTTGCA
    TGGCTTGGCT GGTACACAGG AATGCTTTTC CCAGCAGCCA TTGTTGGCTT GTTTGTTTTC
    CTCTATGGAA TGTTTACACT AGACAGCTGC CAAGTCAGTC GAGAAATCTG CCAAGCCACA
    AACATTACTA TGTGTCCACT ATGTGACAAA TATTGTCCAT ACATGAGGTT GTCAGACAGT
    TGCATCTATG CCAAGGTTAC CCATCTGTTT GACAACAGTG CAACAGTATT CTTTGCTGTT
    TTTATGGCAG TTTGGGCCAC AGTGTTTCTG GAGTTTTGGA AAAGAAGAAG AGCAGTAATT
    GCTTATGACT GGGACTTAAT AGACTGGGAA GAAGAAGAGG AAGAAATCCG CCCTCAGTTT
    GAAGCCAAAT ACTCAAAGAA AGAGCGTGTA AACCCAATAT CAGGAAAGCC AGAGCCTTAT
    CAAGCCTTTG CAGATAAATG CAGCAGACTT GTTGTTTCTG CTTCTGGAAT ATTTTTTATG
    ATATGTGTCG TAATTGCTGC AGTTTTCGGC ATTGTTATTT ATCGTGTTGT GACTGTCAGT
    ACGTTTGCTG CCTTTAAGTG GGCGTTAATA AGGAACAATT CTCAGGTTGC TACTACAGGG
    ACGGCGGTGT GCATAAACTT TTGCATTATA ATGCTTCTGA ATGTGCTCTA TGAAAGGGTT
    GCACTCTTCC TTACAAACCT AGAACAGCCT CGCACTGAAT CAGAATGGGA GAACAGCTTT
    ACTTTGAAAA TGTTTCTTTT TCAGTTTGTC AATCTGAACA GTTCCACCTT TTACATTGCA
    TTCTTTCTTG GGCGGTTTAC TGGCAGACCA GGGGCTTATT TAAGGCTCAT AAACAAATGG
    CGACTTGAAG AGTGTCACCC AAGTGGATGC CTTATTGACC TATGCCTGCA GATGGGAATA
    ATTATGGTTC TCAAACAGAC CTGGAATAAC TTCATGGAAC TTGGGTACCC ACTTATTCAG
    AACTGGTGGA CTCGAAGAAA ATTACGTCAA GAGCATGGAA TTCATGGAAA AACTGCCCTT
    CCACAGTGGG AAAAGGACTA CAACTTGCAG CCAATCAATC CATATGGACT CTTTGAAGAG
    TATCTGGAAA TGATTCTCCA GTTTGGCTTC ACAACCATCT TTGTGGCTGC CTTTCCATTA
    GCTCCTCTAC TTGCCCTACT TAACAACATC ATTGAAATCC GACTTGATGC ATATAAATTC
    ATCACTCAGT GGAGGCGGCC TCTAGCTTCC CGGGCAAAGG ATATAGGCAT ATGGTATGGA
    ATTCTTGAAG GCATCGGAAT TCTCTCAGTT ATCACCAACG CTTTTGTCAT AGCTGTGACC
    TCGGACTTCA TCCCTCGTCT GGTCTACGCT TACAAATATG GACCCTGTGC AGGTCAAGGA
    CGCGCTGGAG AAAAGTGCAT GGTCGGTTAT GTTAATGCCA GTCTTTCAGT ATTTTTGGTG
    ACTGATTTTG AAAACCATTC AAATTCTTCA CGCAATGGAA GAGAATTTGC TGAACAATAT
    GTAAAATATT GCAGGTACCG TGACTATAGA GACCCCCCAA GCGCTCCTGA GCCCTACGCT
    TATACACTGC AGTTCTGGCA TGTTTTAGCA GCTCGATTGG CATTCATTAT TGTATTTGAG
    CATCTTGTCT TTTTCATAAA GCATCTGATC TCTTACCTAA TACCAGACCT TCCCAAGGAC
    CTAAGGGACA GGATGAGAAG AGAAAAATAC CTTATACAGG AGATGATGTA TGAAGCAGAG
    CTAGAGAGAG TCCAAAAAGA AAAGAAAGAA AGAAAAAAAA ATGGAAAATG TCAACATAAT
    GAGTGGCCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815312.1
    CDS412..3183
    Translation

    Target ORF information:

    RefSeq Version XM_012959858.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis anoctamin 4 (ano4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959858.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGGCCT CCGGCAAATG TGCACTCTCA AATGGGAACA CAGAAATTCA GTACCCAGAA 
    GTTAAAGATA TAAATATATT CTTTGATGAT TTAGAGGCTG CAAACAGTCC ATCAAAAGAT
    GATGATTCTT TGCTGCATGC TGGCAATTTA ACAAGCATGT CAGATGAAGT CAGCCGGTTG
    GACGCAGAAG GAGAGGTTTT GAGTGCTCCA GAAAAAAATA AAACAAGTGG CCTATTTTTT
    AATGATGGGA AGCGTCGTAT TGACTATATC CTGGTGTACA GAAAAACCAG TCTTCAGTCA
    GAAAAAAGGG AGATTTTTGA AAGAAATATT CGGGCGGAGG GATTGCATTT AGAGAAGGAG
    TCTTCCATTG TGAACAGTGA CATAATATTT GTAAAACTTC ATGCTCCATG GGAGGTACTA
    GGAAGATACG CAGAACTAAT GAATGTAAGG ATGCCTTTCA GGAGAAAAAT CTATTACCTG
    CACCGTCGGT ACAAGTTCAT GAGTAGGATG GAGAAGCAAT TGAGCAGGTT TCGAGGTTTG
    TTACCTAAAA AGCCCATGAG ACTGGACAAA GAGAAAATTC CAGACCTGGA GGAGAATGAC
    TGTTACACTG CGCCATTTAG TCAACAGAGG ATTCACCACT TCATAATAAA CAATAAAGAC
    ACATTTTTCA ACAATGCCAC AAGGACCAGA ATTGTACATC ATATTTTACA AAGAGTCAAA
    TATGAAGAGG GGAAAAATAA AATCGGACTG AACCGATTGC TCACAAACTG CACATATGAA
    GCTGCCTTCC CCCTACATGA GGGGAGTTAC AGAAGCAAAA ATTCAATAAA GACACATGGA
    GCAGAAAATC ACAGACATTT GCTTTTTGAA TGTTGGGCCT CCTGGGGAGT ATGGTATAAA
    TACCAACCAC TAGATCTGGT CAGGCGGTAT TTCGGAGAGA AGATAGGTCT GTATTTTGCA
    TGGCTTGGCT GGTACACAGG AATGCTTTTC CCAGCAGCCA TTGTTGGCTT GTTTGTTTTC
    CTCTATGGAA TGTTTACACT AGACAGCTGC CAAGTCAGTC GAGAAATCTG CCAAGCCACA
    AACATTACTA TGTGTCCACT ATGTGACAAA TATTGTCCAT ACATGAGGTT GTCAGACAGT
    TGCATCTATG CCAAGGTTAC CCATCTGTTT GACAACAGTG CAACAGTATT CTTTGCTGTT
    TTTATGGCAG TTTGGGCCAC AGTGTTTCTG GAGTTTTGGA AAAGAAGAAG AGCAGTAATT
    GCTTATGACT GGGACTTAAT AGACTGGGAA GAAGAAGAGG AAGAAATCCG CCCTCAGTTT
    GAAGCCAAAT ACTCAAAGAA AGAGCGTGTA AACCCAATAT CAGGAAAGCC AGAGCCTTAT
    CAAGCCTTTG CAGATAAATG CAGCAGACTT GTTGTTTCTG CTTCTGGAAT ATTTTTTATG
    ATATGTGTCG TAATTGCTGC AGTTTTCGGC ATTGTTATTT ATCGTGTTGT GACTGTCAGT
    ACGTTTGCTG CCTTTAAGTG GGCGTTAATA AGGAACAATT CTCAGGTTGC TACTACAGGG
    ACGGCGGTGT GCATAAACTT TTGCATTATA ATGCTTCTGA ATGTGCTCTA TGAAAGGGTT
    GCACTCTTCC TTACAAACCT AGAACAGCCT CGCACTGAAT CAGAATGGGA GAACAGCTTT
    ACTTTGAAAA TGTTTCTTTT TCAGTTTGTC AATCTGAACA GTTCCACCTT TTACATTGCA
    TTCTTTCTTG GGCGGTTTAC TGGCAGACCA GGGGCTTATT TAAGGCTCAT AAACAAATGG
    CGACTTGAAG AGTGTCACCC AAGTGGATGC CTTATTGACC TATGCCTGCA GATGGGAATA
    ATTATGGTTC TCAAACAGAC CTGGAATAAC TTCATGGAAC TTGGGTACCC ACTTATTCAG
    AACTGGTGGA CTCGAAGAAA ATTACGTCAA GAGCATGGAA TTCATGGAAA AACTGCCCTT
    CCACAGTGGG AAAAGGACTA CAACTTGCAG CCAATCAATC CATATGGACT CTTTGAAGAG
    TATCTGGAAA TGATTCTCCA GTTTGGCTTC ACAACCATCT TTGTGGCTGC CTTTCCATTA
    GCTCCTCTAC TTGCCCTACT TAACAACATC ATTGAAATCC GACTTGATGC ATATAAATTC
    ATCACTCAGT GGAGGCGGCC TCTAGCTTCC CGGGCAAAGG ATATAGGCAT ATGGTATGGA
    ATTCTTGAAG GCATCGGAAT TCTCTCAGTT ATCACCAACG CTTTTGTCAT AGCTGTGACC
    TCGGACTTCA TCCCTCGTCT GGTCTACGCT TACAAATATG GACCCTGTGC AGGTCAAGGA
    CGCGCTGGAG AAAAGTGCAT GGTCGGTTAT GTTAATGCCA GTCTTTCAGT ATTTTTGGTG
    ACTGATTTTG AAAACCATTC AAATTCTTCA CGCAATGGAA GAGAATTTGC TGAACAATAT
    GTAAAATATT GCAGGTACCG TGACTATAGA GACCCCCCAA GCGCTCCTGA GCCCTACGCT
    TATACACTGC AGTTCTGGCA TGTTTTAGCA GCTCGATTGG CATTCATTAT TGTATTTGAG
    CATCTTGTCT TTTTCATAAA GCATCTGATC TCTTACCTAA TACCAGACCT TCCCAAGGAC
    CTAAGGGACA GGATGAGAAG AGAAAAATAC CTTATACAGG AGATGATGTA TGAAGCAGAG
    CTAGAGAGAG TCCAAAAAGA AAAGAAAGAA AGAAAAAAAA ATGGAAAATG TCAACATAAT
    GAGTGGCCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54601
    Clone ID Related Accession (Same CDS sequence) XM_012959858.3
    Accession Version XM_012959858.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product anoctamin-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959858.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGGCCT CCGGCAAATG TGCACTCTCA AATGGGAACA CAGAAATTCA GTACCCAGGA 
    GTTAAAGATA TAAATATATT CTTTGATGAT TTAGAGGCTG CAAACAGTCC ATCAAAAGAT
    GATGATTCTT TGCTGCATGC TGGCAATTTA ACAAGCATGT CAGATGAAGT CAGCCGGTTG
    GACGCAGAAG GAGAGGTTTT GAGTGCTCCA GAAAAAAATA AAACAAGTGG CCTATTTTTT
    AATGATGGGA AGCGTCGTAT TGACTATATC CTGGTGTACA GAAAAACCAG TCTTCAGTCA
    GAAAAAAGGG AGATTTTTGA AAGAAATATT CGGGCGGAGG GATTGCATTT AGAGAAGGAG
    TCTTCCATTG TGAACAGTGA CATAATATTT GTAAAACTTC ATGCTCCATG GGAGGTACTA
    GGAAGATACG CAGAACTAAT GAATGTAAGG ATGCCTTTCA GGAGAAAAAT CTATTACCTG
    CACCGTCGGT ACAAGTTCAT GAGTAGGATG GAGAAGCAAT TGAGCAGGTT TCGAGGTTTG
    TTACCTAAAA AGCCCATGAG ACTGGACAAA GAGAAAATTC CAGACCTGGA GGAGAATGAC
    TGTTACACTG CGCCATTTAG TCAACAGAGG ATTCACCACT TCATAATAAA CAATAAAGAC
    ACATTTTTCA ACAATGCCAC AAGGACCAGA ATTGTACATC ATATTTTACA AAGAGTCAAA
    TATGAAGAGG GGAAAAATAA AATCGGACTG AACCGATTGC TCACAAACTG CACATATGAA
    GCTGCCTTCC CCCTACATGA GGGGAGTTAC AGAAGCAAAA ATTCAATAAA GACACATGGA
    GCAGAAAATC ACAGACATTT GCTTTTTGAA TGTTGGGCCT CCTGGGGAGT ATGGTATAAA
    TACCAACCAC TAGATCTGGT CAGGCGGTAT TTCGGAGAGA AGATAGGTCT GTATTTTGCA
    TGGCTTGGCT GGTACACAGG AATGCTTTTC CCAGCAGCCA TTGTTGGCTT GTTTGTTTTC
    CTCTATGGAA TGTTTACACT AGACAGCTGC CAAGTCAGTC GAGAAATCTG CCAAGCCACA
    AACATTACTA TGTGTCCACT ATGTGACAAA TATTGTCCAT ACATGAGGTT GTCAGACAGT
    TGCATCTATG CCAAGGTTAC CCATCTGTTT GACAACAGTG CAACAGTATT CTTTGCTGTT
    TTTATGGCAG TTTGGGCCAC AGTGTTTCTG GAGTTTTGGA AAAGAAGAAG AGCAGTAATT
    GCTTATGACT GGGACTTAAT AGACTGGGAA GAAGAAGAGG AAGAAATCCG CCCTCAGTTT
    GAAGCCAAAT ACTCAAAGAA AGAGCGTGTA AACCCAATAT CAGGAAAGCC AGAGCCTTAT
    CAAGCCTTTG CAGATAAATG CAGCAGACTT GTTGTTTCTG CTTCTGGAAT ATTTTTTATG
    ATATGTGTCG TAATTGCTGC AGTTTTCGGC ATTGTTATTT ATCGTGTTGT GACTGTCAGT
    ACGTTTGCTG CCTTTAAGTG GGCGTTAATA AGGAACAATT CTCAGGTTGC TACTACAGGG
    ACGGCGGTGT GCATAAACTT TTGCATTATA ATGCTTCTGA ATGTGCTCTA TGAAAGGGTT
    GCACTCTTCC TTACAAACCT AGAACAGCCT CGCACTGAAT CAGAATGGGA GAACAGCTTT
    ACTTTGAAAA TGTTTCTTTT TCAGTTTGTC AATCTGAACA GTTCCACCTT TTACATTGCA
    TTCTTTCTTG GGCGGTTTAC TGGCAGACCA GGGGCTTATT TAAGGCTCAT AAACAAATGG
    CGACTTGAAG AGTGTCACCC AAGTGGATGC CTTATTGACC TATGCCTGCA GATGGGAATA
    ATTATGGTTC TCAAACAGAC CTGGAATAAC TTCATGGAAC TTGGGTACCC ACTTATTCAG
    AACTGGTGGA CTCGAAGAAA ATTACGTCAA GAGCATGGAA TTCATGGAAA AACTGCCCTT
    CCACAGTGGG AAAAGGACTA CAACTTGCAG CCAATCAATC CATATGGACT CTTTGAAGAG
    TATCTGGAAA TGATTCTCCA GTTTGGCTTC ACAACCATCT TTGTGGCTGC CTTTCCATTA
    GCTCCTCTAC TTGCCCTACT TAACAACATC ATTGAAATCC GACTTGATGC ATATAAATTC
    ATCACTCAGT GGAGGCGGCC TCTAGCTTCC CGGGCAAAGG ATATAGGCAT ATGGTATGGA
    ATTCTTGAAG GCATCGGAAT TCTCTCAGTT ATCACCAACG CTTTTGTCAT AGCTGTGACC
    TCGGACTTCA TCCCTCGTCT GGTCTACGCT TACAAATATG GACCCTGTGC AGGTCAAGGA
    CGCGCTGGAG AAAAGTGCAT GGTCGGTTAT GTTAATGCCA GTCTTTCAGT ATTTTTGGTG
    ACTGATTTTG AAAACCATTC AAATTCTTCA CGCAATGGAA GAGAATTTGC TGAACAATAT
    GTAAAATATT GCAGGTACCG TGACTATAGA GACCCCCCAA GCGCTCCTGA GCCCTACGCT
    TATACACTGC AGTTCTGGCA TGTTTTAGCA GCTCGATTGG CATTCATTAT TGTATTTGAG
    CATCTTGTCT TTTTCATAAA GCATCTGATC TCTTACCTAA TACCAGACCT TCCCAAGGAC
    CTAAGGGACA GGATGAGAAG AGAAAAATAC CTTATACAGG AGATGATGTA TGAAGCAGAG
    CTAGAGAGAG TCCAAAAAGA AAAGAAAGAA AGAAAAAAAA ATGGAAAATG TCAACATAAT
    GAGTGGCCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815312.2
    CDS564..3335
    Translation

    Target ORF information:

    RefSeq Version XM_012959858.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis anoctamin 4 (ano4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959858.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGGCCT CCGGCAAATG TGCACTCTCA AATGGGAACA CAGAAATTCA GTACCCAGGA 
    GTTAAAGATA TAAATATATT CTTTGATGAT TTAGAGGCTG CAAACAGTCC ATCAAAAGAT
    GATGATTCTT TGCTGCATGC TGGCAATTTA ACAAGCATGT CAGATGAAGT CAGCCGGTTG
    GACGCAGAAG GAGAGGTTTT GAGTGCTCCA GAAAAAAATA AAACAAGTGG CCTATTTTTT
    AATGATGGGA AGCGTCGTAT TGACTATATC CTGGTGTACA GAAAAACCAG TCTTCAGTCA
    GAAAAAAGGG AGATTTTTGA AAGAAATATT CGGGCGGAGG GATTGCATTT AGAGAAGGAG
    TCTTCCATTG TGAACAGTGA CATAATATTT GTAAAACTTC ATGCTCCATG GGAGGTACTA
    GGAAGATACG CAGAACTAAT GAATGTAAGG ATGCCTTTCA GGAGAAAAAT CTATTACCTG
    CACCGTCGGT ACAAGTTCAT GAGTAGGATG GAGAAGCAAT TGAGCAGGTT TCGAGGTTTG
    TTACCTAAAA AGCCCATGAG ACTGGACAAA GAGAAAATTC CAGACCTGGA GGAGAATGAC
    TGTTACACTG CGCCATTTAG TCAACAGAGG ATTCACCACT TCATAATAAA CAATAAAGAC
    ACATTTTTCA ACAATGCCAC AAGGACCAGA ATTGTACATC ATATTTTACA AAGAGTCAAA
    TATGAAGAGG GGAAAAATAA AATCGGACTG AACCGATTGC TCACAAACTG CACATATGAA
    GCTGCCTTCC CCCTACATGA GGGGAGTTAC AGAAGCAAAA ATTCAATAAA GACACATGGA
    GCAGAAAATC ACAGACATTT GCTTTTTGAA TGTTGGGCCT CCTGGGGAGT ATGGTATAAA
    TACCAACCAC TAGATCTGGT CAGGCGGTAT TTCGGAGAGA AGATAGGTCT GTATTTTGCA
    TGGCTTGGCT GGTACACAGG AATGCTTTTC CCAGCAGCCA TTGTTGGCTT GTTTGTTTTC
    CTCTATGGAA TGTTTACACT AGACAGCTGC CAAGTCAGTC GAGAAATCTG CCAAGCCACA
    AACATTACTA TGTGTCCACT ATGTGACAAA TATTGTCCAT ACATGAGGTT GTCAGACAGT
    TGCATCTATG CCAAGGTTAC CCATCTGTTT GACAACAGTG CAACAGTATT CTTTGCTGTT
    TTTATGGCAG TTTGGGCCAC AGTGTTTCTG GAGTTTTGGA AAAGAAGAAG AGCAGTAATT
    GCTTATGACT GGGACTTAAT AGACTGGGAA GAAGAAGAGG AAGAAATCCG CCCTCAGTTT
    GAAGCCAAAT ACTCAAAGAA AGAGCGTGTA AACCCAATAT CAGGAAAGCC AGAGCCTTAT
    CAAGCCTTTG CAGATAAATG CAGCAGACTT GTTGTTTCTG CTTCTGGAAT ATTTTTTATG
    ATATGTGTCG TAATTGCTGC AGTTTTCGGC ATTGTTATTT ATCGTGTTGT GACTGTCAGT
    ACGTTTGCTG CCTTTAAGTG GGCGTTAATA AGGAACAATT CTCAGGTTGC TACTACAGGG
    ACGGCGGTGT GCATAAACTT TTGCATTATA ATGCTTCTGA ATGTGCTCTA TGAAAGGGTT
    GCACTCTTCC TTACAAACCT AGAACAGCCT CGCACTGAAT CAGAATGGGA GAACAGCTTT
    ACTTTGAAAA TGTTTCTTTT TCAGTTTGTC AATCTGAACA GTTCCACCTT TTACATTGCA
    TTCTTTCTTG GGCGGTTTAC TGGCAGACCA GGGGCTTATT TAAGGCTCAT AAACAAATGG
    CGACTTGAAG AGTGTCACCC AAGTGGATGC CTTATTGACC TATGCCTGCA GATGGGAATA
    ATTATGGTTC TCAAACAGAC CTGGAATAAC TTCATGGAAC TTGGGTACCC ACTTATTCAG
    AACTGGTGGA CTCGAAGAAA ATTACGTCAA GAGCATGGAA TTCATGGAAA AACTGCCCTT
    CCACAGTGGG AAAAGGACTA CAACTTGCAG CCAATCAATC CATATGGACT CTTTGAAGAG
    TATCTGGAAA TGATTCTCCA GTTTGGCTTC ACAACCATCT TTGTGGCTGC CTTTCCATTA
    GCTCCTCTAC TTGCCCTACT TAACAACATC ATTGAAATCC GACTTGATGC ATATAAATTC
    ATCACTCAGT GGAGGCGGCC TCTAGCTTCC CGGGCAAAGG ATATAGGCAT ATGGTATGGA
    ATTCTTGAAG GCATCGGAAT TCTCTCAGTT ATCACCAACG CTTTTGTCAT AGCTGTGACC
    TCGGACTTCA TCCCTCGTCT GGTCTACGCT TACAAATATG GACCCTGTGC AGGTCAAGGA
    CGCGCTGGAG AAAAGTGCAT GGTCGGTTAT GTTAATGCCA GTCTTTCAGT ATTTTTGGTG
    ACTGATTTTG AAAACCATTC AAATTCTTCA CGCAATGGAA GAGAATTTGC TGAACAATAT
    GTAAAATATT GCAGGTACCG TGACTATAGA GACCCCCCAA GCGCTCCTGA GCCCTACGCT
    TATACACTGC AGTTCTGGCA TGTTTTAGCA GCTCGATTGG CATTCATTAT TGTATTTGAG
    CATCTTGTCT TTTTCATAAA GCATCTGATC TCTTACCTAA TACCAGACCT TCCCAAGGAC
    CTAAGGGACA GGATGAGAAG AGAAAAATAC CTTATACAGG AGATGATGTA TGAAGCAGAG
    CTAGAGAGAG TCCAAAAAGA AAAGAAAGAA AGAAAAAAAA ATGGAAAATG TCAACATAAT
    GAGTGGCCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54602
    Clone ID Related Accession (Same CDS sequence) XM_031898856.1
    Accession Version XM_031898856.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product anoctamin-4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGTTTTC TTCTCGACTG GCTTCATTTT TGCTGTTCTT CCAGGAGAAA AATCTATTAC 
    CTGCACCGTC GGTACAAGTT CATGAGTAGG ATGGAGAAGC AATTGAGCAG GTTTCGAGGT
    TTGTTACCTA AAAAGCCCAT GAGACTGGAC AAAGAGAAAA TTCCAGACCT GGAGGAGAAT
    GACTGTTACA CTGCGCCATT TAGTCAACAG AGGATTCACC ACTTCATAAT AAACAATAAA
    GACACATTTT TCAACAATGC CACAAGGACC AGAATTGTAC ATCATATTTT ACAAAGAGTC
    AAATATGAAG AGGGGAAAAA TAAAATCGGA CTGAACCGAT TGCTCACAAA CTGCACATAT
    GAAGCTGCCT TCCCCCTACA TGAGGGGAGT TACAGAAGCA AAAATTCAAT AAAGACACAT
    GGAGCAGAAA ATCACAGACA TTTGCTTTTT GAATGTTGGG CCTCCTGGGG AGTATGGTAT
    AAATACCAAC CACTAGATCT GGTCAGGCGG TATTTCGGAG AGAAGATAGG TCTGTATTTT
    GCATGGCTTG GCTGGTACAC AGGAATGCTT TTCCCAGCAG CCATTGTTGG CTTGTTTGTT
    TTCCTCTATG GAATGTTTAC ACTAGACAGC TGCCAAGTCA GTCGAGAAAT CTGCCAAGCC
    ACAAACATTA CTATGTGTCC ACTATGTGAC AAATATTGTC CATACATGAG GTTGTCAGAC
    AGTTGCATCT ATGCCAAGGT TACCCATCTG TTTGACAACA GTGCAACAGT ATTCTTTGCT
    GTTTTTATGG CAGTTTGGGC CACAGTGTTT CTGGAGTTTT GGAAAAGAAG AAGAGCAGTA
    ATTGCTTATG ACTGGGACTT AATAGACTGG GAAGAAGAAG AGGAAGAAAT CCGCCCTCAG
    TTTGAAGCCA AATACTCAAA GAAAGAGCGT GTAAACCCAA TATCAGGAAA GCCAGAGCCT
    TATCAAGCCT TTGCAGATAA ATGCAGCAGA CTTGTTGTTT CTGCTTCTGG AATATTTTTT
    ATGATATGTG TCGTAATTGC TGCAGTTTTC GGCATTGTTA TTTATCGTGT TGTGACTGTC
    AGTACGTTTG CTGCCTTTAA GTGGGCGTTA ATAAGGAACA ATTCTCAGGT TGCTACTACA
    GGGACGGCGG TGTGCATAAA CTTTTGCATT ATAATGCTTC TGAATGTGCT CTATGAAAGG
    GTTGCACTCT TCCTTACAAA CCTAGAACAG CCTCGCACTG AATCAGAATG GGAGAACAGC
    TTTACTTTGA AAATGTTTCT TTTTCAGTTT GTCAATCTGA ACAGTTCCAC CTTTTACATT
    GCATTCTTTC TTGGGCGGTT TACTGGCAGA CCAGGGGCTT ATTTAAGGCT CATAAACAAA
    TGGCGACTTG AAGAGTGTCA CCCAAGTGGA TGCCTTATTG ACCTATGCCT GCAGATGGGA
    ATAATTATGG TTCTCAAACA GACCTGGAAT AACTTCATGG AACTTGGGTA CCCACTTATT
    CAGAACTGGT GGACTCGAAG AAAATTACGT CAAGAGCATG GAATTCATGG AAAAACTGCC
    CTTCCACAGT GGGAAAAGGA CTACAACTTG CAGCCAATCA ATCCATATGG ACTCTTTGAA
    GAGTATCTGG AAATGATTCT CCAGTTTGGC TTCACAACCA TCTTTGTGGC TGCCTTTCCA
    TTAGCTCCTC TACTTGCCCT ACTTAACAAC ATCATTGAAA TCCGACTTGA TGCATATAAA
    TTCATCACTC AGTGGAGGCG GCCTCTAGCT TCCCGGGCAA AGGATATAGG CATATGGTAT
    GGAATTCTTG AAGGCATCGG AATTCTCTCA GTTATCACCA ACGCTTTTGT CATAGCTGTG
    ACCTCGGACT TCATCCCTCG TCTGGTCTAC GCTTACAAAT ATGGACCCTG TGCAGGTCAA
    GGACGCGCTG GAGAAAAGTG CATGGTCGGT TATGTTAATG CCAGTCTTTC AGTATTTTTG
    GTGACTGATT TTGAAAACCA TTCAAATTCT TCACGCAATG GAAGAGAATT TGCTGAACAA
    TATGTAAAAT ATTGCAGGTA CCGTGACTAT AGAGACCCCC CAAGCGCTCC TGAGCCCTAC
    GCTTATACAC TGCAGTTCTG GCATGTTTTA GCAGCTCGAT TGGCATTCAT TATTGTATTT
    GAGCATCTTG TCTTTTTCAT AAAGCATCTG ATCTCTTACC TAATACCAGA CCTTCCCAAG
    GACCTAAGGG ACAGGATGAG AAGAGAAAAA TACCTTATAC AGGAGATGAT GTATGAAGCA
    GAGCTAGAGA GAGTCCAAAA AGAAAAGAAA GAAAGAAAAA AAAATGGAAA ATGTCAACAT
    AATGAGTGGC CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754716.1
    CDS109..2463
    Translation

    Target ORF information:

    RefSeq Version XM_031898856.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis anoctamin 4 (ano4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898856.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGGTTTTC TTCTCGACTG GCTTCATTTT TGCTGTTCTT CCAGGAGAAA AATCTATTAC 
    CTGCACCGTC GGTACAAGTT CATGAGTAGG ATGGAGAAGC AATTGAGCAG GTTTCGAGGT
    TTGTTACCTA AAAAGCCCAT GAGACTGGAC AAAGAGAAAA TTCCAGACCT GGAGGAGAAT
    GACTGTTACA CTGCGCCATT TAGTCAACAG AGGATTCACC ACTTCATAAT AAACAATAAA
    GACACATTTT TCAACAATGC CACAAGGACC AGAATTGTAC ATCATATTTT ACAAAGAGTC
    AAATATGAAG AGGGGAAAAA TAAAATCGGA CTGAACCGAT TGCTCACAAA CTGCACATAT
    GAAGCTGCCT TCCCCCTACA TGAGGGGAGT TACAGAAGCA AAAATTCAAT AAAGACACAT
    GGAGCAGAAA ATCACAGACA TTTGCTTTTT GAATGTTGGG CCTCCTGGGG AGTATGGTAT
    AAATACCAAC CACTAGATCT GGTCAGGCGG TATTTCGGAG AGAAGATAGG TCTGTATTTT
    GCATGGCTTG GCTGGTACAC AGGAATGCTT TTCCCAGCAG CCATTGTTGG CTTGTTTGTT
    TTCCTCTATG GAATGTTTAC ACTAGACAGC TGCCAAGTCA GTCGAGAAAT CTGCCAAGCC
    ACAAACATTA CTATGTGTCC ACTATGTGAC AAATATTGTC CATACATGAG GTTGTCAGAC
    AGTTGCATCT ATGCCAAGGT TACCCATCTG TTTGACAACA GTGCAACAGT ATTCTTTGCT
    GTTTTTATGG CAGTTTGGGC CACAGTGTTT CTGGAGTTTT GGAAAAGAAG AAGAGCAGTA
    ATTGCTTATG ACTGGGACTT AATAGACTGG GAAGAAGAAG AGGAAGAAAT CCGCCCTCAG
    TTTGAAGCCA AATACTCAAA GAAAGAGCGT GTAAACCCAA TATCAGGAAA GCCAGAGCCT
    TATCAAGCCT TTGCAGATAA ATGCAGCAGA CTTGTTGTTT CTGCTTCTGG AATATTTTTT
    ATGATATGTG TCGTAATTGC TGCAGTTTTC GGCATTGTTA TTTATCGTGT TGTGACTGTC
    AGTACGTTTG CTGCCTTTAA GTGGGCGTTA ATAAGGAACA ATTCTCAGGT TGCTACTACA
    GGGACGGCGG TGTGCATAAA CTTTTGCATT ATAATGCTTC TGAATGTGCT CTATGAAAGG
    GTTGCACTCT TCCTTACAAA CCTAGAACAG CCTCGCACTG AATCAGAATG GGAGAACAGC
    TTTACTTTGA AAATGTTTCT TTTTCAGTTT GTCAATCTGA ACAGTTCCAC CTTTTACATT
    GCATTCTTTC TTGGGCGGTT TACTGGCAGA CCAGGGGCTT ATTTAAGGCT CATAAACAAA
    TGGCGACTTG AAGAGTGTCA CCCAAGTGGA TGCCTTATTG ACCTATGCCT GCAGATGGGA
    ATAATTATGG TTCTCAAACA GACCTGGAAT AACTTCATGG AACTTGGGTA CCCACTTATT
    CAGAACTGGT GGACTCGAAG AAAATTACGT CAAGAGCATG GAATTCATGG AAAAACTGCC
    CTTCCACAGT GGGAAAAGGA CTACAACTTG CAGCCAATCA ATCCATATGG ACTCTTTGAA
    GAGTATCTGG AAATGATTCT CCAGTTTGGC TTCACAACCA TCTTTGTGGC TGCCTTTCCA
    TTAGCTCCTC TACTTGCCCT ACTTAACAAC ATCATTGAAA TCCGACTTGA TGCATATAAA
    TTCATCACTC AGTGGAGGCG GCCTCTAGCT TCCCGGGCAA AGGATATAGG CATATGGTAT
    GGAATTCTTG AAGGCATCGG AATTCTCTCA GTTATCACCA ACGCTTTTGT CATAGCTGTG
    ACCTCGGACT TCATCCCTCG TCTGGTCTAC GCTTACAAAT ATGGACCCTG TGCAGGTCAA
    GGACGCGCTG GAGAAAAGTG CATGGTCGGT TATGTTAATG CCAGTCTTTC AGTATTTTTG
    GTGACTGATT TTGAAAACCA TTCAAATTCT TCACGCAATG GAAGAGAATT TGCTGAACAA
    TATGTAAAAT ATTGCAGGTA CCGTGACTAT AGAGACCCCC CAAGCGCTCC TGAGCCCTAC
    GCTTATACAC TGCAGTTCTG GCATGTTTTA GCAGCTCGAT TGGCATTCAT TATTGTATTT
    GAGCATCTTG TCTTTTTCAT AAAGCATCTG ATCTCTTACC TAATACCAGA CCTTCCCAAG
    GACCTAAGGG ACAGGATGAG AAGAGAAAAA TACCTTATAC AGGAGATGAT GTATGAAGCA
    GAGCTAGAGA GAGTCCAAAA AGAAAAGAAA GAAAGAAAAA AAAATGGAAA ATGTCAACAT
    AATGAGTGGC CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54599
    Clone ID Related Accession (Same CDS sequence) XM_012959855.3 , XM_004912893.4
    Accession Version XM_012959855.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product anoctamin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959855.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA
    AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC
    TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC
    CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA
    CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG
    CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA
    ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT
    CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC
    ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT
    TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC
    CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG
    CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA
    AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA
    AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA
    GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815309.2
    CDS269..3742
    Translation

    Target ORF information:

    RefSeq Version XM_012959855.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis anoctamin 4 (ano4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959855.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA
    AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC
    TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC
    CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA
    CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG
    CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA
    ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT
    CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC
    ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT
    TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC
    CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG
    CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA
    AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA
    AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA
    GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54599
    Clone ID Related Accession (Same CDS sequence) XM_012959855.3 , XM_004912893.4
    Accession Version XM_004912893.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product anoctamin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912893.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA
    AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC
    TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC
    CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA
    CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG
    CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA
    ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT
    CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC
    ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT
    TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC
    CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG
    CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA
    AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA
    AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA
    GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912950.2
    CDS269..3742
    Translation

    Target ORF information:

    RefSeq Version XM_004912893.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis anoctamin 4 (ano4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912893.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA
    AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC
    TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC
    CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA
    CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG
    CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA
    ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT
    CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC
    ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT
    TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC
    CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG
    CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA
    AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA
    AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA
    GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54600
    Clone ID Related Accession (Same CDS sequence) XM_012959856.3
    Accession Version XM_012959856.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product anoctamin-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGACTT ATTCAGAACT GGTGGACTCG AAGAAAATTA
    CGTCAAGAGC ATGGAATTCA TGGAAAAACT GCCCTTCCAC AGTGGGAAAA GGACTACAAC
    TTGCAGCCAA TCAATCCATA TGGACTCTTT GAAGAGTATC TGGAAATGAT TCTCCAGTTT
    GGCTTCACAA CCATCTTTGT GGCTGCCTTT CCATTAGCTC CTCTACTTGC CCTACTTAAC
    AACATCATTG AAATCCGACT TGATGCATAT AAATTCATCA CTCAGTGGAG GCGGCCTCTA
    GCTTCCCGGG CAAAGGATAT AGGCATATGG TATGGAATTC TTGAAGGCAT CGGAATTCTC
    TCAGTTATCA CCAACGCTTT TGTCATAGCT GTGACCTCGG ACTTCATCCC TCGTCTGGTC
    TACGCTTACA AATATGGACC CTGTGCAGGT CAAGGACGCG CTGGAGAAAA GTGCATGGTC
    GGTTATGTTA ATGCCAGTCT TTCAGTATTT TTGGTGACTG ATTTTGAAAA CCATTCAAAT
    TCTTCACGCA ATGGAAGAGA ATTTGCTGAA CAATATGTAA AATATTGCAG GTACCGTGAC
    TATAGAGACC CCCCAAGCGC TCCTGAGCCC TACGCTTATA CACTGCAGTT CTGGCATGTT
    TTAGCAGCTC GATTGGCATT CATTATTGTA TTTGAGCATC TTGTCTTTTT CATAAAGCAT
    CTGATCTCTT ACCTAATACC AGACCTTCCC AAGGACCTAA GGGACAGGAT GAGAAGAGAA
    AAATACCTTA TACAGGAGAT GATGTATGAA GCAGAGCTAG AGAGAGTCCA AAAAGAAAAG
    AAAGAAAGAA AAAAAAATGG AAAATGTCAA CATAATGAGT GGCCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815310.2
    CDS269..3736
    Translation

    Target ORF information:

    RefSeq Version XM_012959856.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis anoctamin 4 (ano4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959856.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGTGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG GAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGACTT ATTCAGAACT GGTGGACTCG AAGAAAATTA
    CGTCAAGAGC ATGGAATTCA TGGAAAAACT GCCCTTCCAC AGTGGGAAAA GGACTACAAC
    TTGCAGCCAA TCAATCCATA TGGACTCTTT GAAGAGTATC TGGAAATGAT TCTCCAGTTT
    GGCTTCACAA CCATCTTTGT GGCTGCCTTT CCATTAGCTC CTCTACTTGC CCTACTTAAC
    AACATCATTG AAATCCGACT TGATGCATAT AAATTCATCA CTCAGTGGAG GCGGCCTCTA
    GCTTCCCGGG CAAAGGATAT AGGCATATGG TATGGAATTC TTGAAGGCAT CGGAATTCTC
    TCAGTTATCA CCAACGCTTT TGTCATAGCT GTGACCTCGG ACTTCATCCC TCGTCTGGTC
    TACGCTTACA AATATGGACC CTGTGCAGGT CAAGGACGCG CTGGAGAAAA GTGCATGGTC
    GGTTATGTTA ATGCCAGTCT TTCAGTATTT TTGGTGACTG ATTTTGAAAA CCATTCAAAT
    TCTTCACGCA ATGGAAGAGA ATTTGCTGAA CAATATGTAA AATATTGCAG GTACCGTGAC
    TATAGAGACC CCCCAAGCGC TCCTGAGCCC TACGCTTATA CACTGCAGTT CTGGCATGTT
    TTAGCAGCTC GATTGGCATT CATTATTGTA TTTGAGCATC TTGTCTTTTT CATAAAGCAT
    CTGATCTCTT ACCTAATACC AGACCTTCCC AAGGACCTAA GGGACAGGAT GAGAAGAGAA
    AAATACCTTA TACAGGAGAT GATGTATGAA GCAGAGCTAG AGAGAGTCCA AAAAGAAAAG
    AAAGAAAGAA AAAAAAATGG AAAATGTCAA CATAATGAGT GGCCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07689
    Clone ID Related Accession (Same CDS sequence) XM_004912893.3 , XM_012959855.2
    Accession Version XM_004912893.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product anoctamin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA
    AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC
    TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC
    CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA
    CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG
    CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA
    ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT
    CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC
    ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT
    TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC
    CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG
    CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA
    AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA
    AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA
    GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912950.1
    CDS261..3734
    Translation

    Target ORF information:

    RefSeq Version XM_004912893.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis anoctamin 4 (ano4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912893.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA
    AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC
    TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC
    CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA
    CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG
    CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA
    ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT
    CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC
    ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT
    TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC
    CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG
    CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA
    AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA
    AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA
    GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07689
    Clone ID Related Accession (Same CDS sequence) XM_004912893.3 , XM_012959855.2
    Accession Version XM_012959855.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product anoctamin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA
    AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC
    TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC
    CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA
    CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG
    CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA
    ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT
    CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC
    ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT
    TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC
    CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG
    CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA
    AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA
    AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA
    GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815309.1
    CDS261..3734
    Translation

    Target ORF information:

    RefSeq Version XM_012959855.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis anoctamin 4 (ano4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959855.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGGTAC CCACTTATTC AGAACTGGTG GACTCGAAGA
    AAATTACGTC AAGAGCATGG AATTCATGGA AAAACTGCCC TTCCACAGTG GGAAAAGGAC
    TACAACTTGC AGCCAATCAA TCCATATGGA CTCTTTGAAG AGTATCTGGA AATGATTCTC
    CAGTTTGGCT TCACAACCAT CTTTGTGGCT GCCTTTCCAT TAGCTCCTCT ACTTGCCCTA
    CTTAACAACA TCATTGAAAT CCGACTTGAT GCATATAAAT TCATCACTCA GTGGAGGCGG
    CCTCTAGCTT CCCGGGCAAA GGATATAGGC ATATGGTATG GAATTCTTGA AGGCATCGGA
    ATTCTCTCAG TTATCACCAA CGCTTTTGTC ATAGCTGTGA CCTCGGACTT CATCCCTCGT
    CTGGTCTACG CTTACAAATA TGGACCCTGT GCAGGTCAAG GACGCGCTGG AGAAAAGTGC
    ATGGTCGGTT ATGTTAATGC CAGTCTTTCA GTATTTTTGG TGACTGATTT TGAAAACCAT
    TCAAATTCTT CACGCAATGG AAGAGAATTT GCTGAACAAT ATGTAAAATA TTGCAGGTAC
    CGTGACTATA GAGACCCCCC AAGCGCTCCT GAGCCCTACG CTTATACACT GCAGTTCTGG
    CATGTTTTAG CAGCTCGATT GGCATTCATT ATTGTATTTG AGCATCTTGT CTTTTTCATA
    AAGCATCTGA TCTCTTACCT AATACCAGAC CTTCCCAAGG ACCTAAGGGA CAGGATGAGA
    AGAGAAAAAT ACCTTATACA GGAGATGATG TATGAAGCAG AGCTAGAGAG AGTCCAAAAA
    GAAAAGAAAG AAAGAAAAAA AAATGGAAAA TGTCAACATA ATGAGTGGCC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa34171
    Clone ID Related Accession (Same CDS sequence) XM_012959856.2
    Accession Version XM_012959856.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product anoctamin-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGACTT ATTCAGAACT GGTGGACTCG AAGAAAATTA
    CGTCAAGAGC ATGGAATTCA TGGAAAAACT GCCCTTCCAC AGTGGGAAAA GGACTACAAC
    TTGCAGCCAA TCAATCCATA TGGACTCTTT GAAGAGTATC TGGAAATGAT TCTCCAGTTT
    GGCTTCACAA CCATCTTTGT GGCTGCCTTT CCATTAGCTC CTCTACTTGC CCTACTTAAC
    AACATCATTG AAATCCGACT TGATGCATAT AAATTCATCA CTCAGTGGAG GCGGCCTCTA
    GCTTCCCGGG CAAAGGATAT AGGCATATGG TATGGAATTC TTGAAGGCAT CGGAATTCTC
    TCAGTTATCA CCAACGCTTT TGTCATAGCT GTGACCTCGG ACTTCATCCC TCGTCTGGTC
    TACGCTTACA AATATGGACC CTGTGCAGGT CAAGGACGCG CTGGAGAAAA GTGCATGGTC
    GGTTATGTTA ATGCCAGTCT TTCAGTATTT TTGGTGACTG ATTTTGAAAA CCATTCAAAT
    TCTTCACGCA ATGGAAGAGA ATTTGCTGAA CAATATGTAA AATATTGCAG GTACCGTGAC
    TATAGAGACC CCCCAAGCGC TCCTGAGCCC TACGCTTATA CACTGCAGTT CTGGCATGTT
    TTAGCAGCTC GATTGGCATT CATTATTGTA TTTGAGCATC TTGTCTTTTT CATAAAGCAT
    CTGATCTCTT ACCTAATACC AGACCTTCCC AAGGACCTAA GGGACAGGAT GAGAAGAGAA
    AAATACCTTA TACAGGAGAT GATGTATGAA GCAGAGCTAG AGAGAGTCCA AAAAGAAAAG
    AAAGAAAGAA AAAAAAATGG AAAATGTCAA CATAATGAGT GGCCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815310.1
    CDS261..3728
    Translation

    Target ORF information:

    RefSeq Version XM_012959856.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis anoctamin 4 (ano4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959856.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCACTGCC CACATAAGGG AAAGCTAAGC GCTAAGCCGC GCAGCCCCAG GTTCGAGTTC 
    GGGGGAGCTG CGCTGGAGCT AAGGCGCTAC AAGTCCATAG ACACCAAGAA GGGAAATGCC
    GGGGGGTTCA ACTTGGGCTC TCCGCGCCTG CAAGCGCCCA TCTCTGTGCC TACCAGCCCG
    GCTTCTCGGG CATGCAGCAG CCCAAGGGGG TCTCCCCTCA CCGGGTATGA CACAGATCCT
    GCCAGCGAGA GCTACAATAA TCTTTCCAGT GGAACAAGCT ACTACAGCTG TGTCAGCCAG
    ATCACTATAG GATTTGAAGT AAATGGACCA GTTTCAATAG GAATATCACA CTGGAAGCCT
    CCATATGCGC ACAATGAAAC ATATCTCCCC TTCGACCACA GCCAGCACAA TGCAGAGATT
    CCTGCTAACC CAGTCTCATG TTCCTTATTG GCACAGATGG ATTTATCAGC ATCTTTATCT
    TGCCCCACCT CTCTGTCAGT CATACCAGCA GTTTCCAGGA GCAGCAGTCA GGATACACTA
    AGCCAGGACT CTGACATTAA TCAGGGAATA AGCATATCTA TTCATGGGTC TAAAAACCAG
    CTGCAAATCC ATCAAAAGGG AAGTGTGGCC AGCCATTCTC TTCCTAACGA ACAGACTGTG
    GTCGGGCATT CCTCACTTCC GCCACTCAGA AAAGCAATAA AAATGGAGGC CTCCGGCAAA
    TGTGCACTCT CAAATGGGAA CACAGAAATT CAGTACCCAG AAGTTAAAGA TATAAATATA
    TTCTTTGATG ATTTAGAGGC TGCAAACAGT CCATCAAAAG ATGATGATTC TTTGCTGCAT
    GCTGGCAATT TAACAAGCAT GTCAGATGAA GTCAGCCGGT TGGACGCAGA AGGAGAGGTT
    TTGAGTGCTC CAGAAAAAAA TAAAACAAGT GGCCTATTTT TTAATGATGG GAAGCGTCGT
    ATTGACTATA TCCTGGTGTA CAGAAAAACC AGTCTTCAGT CAGAAAAAAG GGAGATTTTT
    GAAAGAAATA TTCGGGCGGA GGGATTGCAT TTAGAGAAGG AGTCTTCCAT TGTGAACAGT
    GACATAATAT TTGTAAAACT TCATGCTCCA TGGGAGGTAC TAGGAAGATA CGCAGAACTA
    ATGAATGTAA GGATGCCTTT CAGGAGAAAA ATCTATTACC TGCACCGTCG GTACAAGTTC
    ATGAGTAGGA TGGAGAAGCA ATTGAGCAGG TTTCGAGGTT TGTTACCTAA AAAGCCCATG
    AGACTGGACA AAGAGAAAAT TCCAGACCTG GAGGAGAATG ACTGTTACAC TGCGCCATTT
    AGTCAACAGA GGATTCACCA CTTCATAATA AACAATAAAG ACACATTTTT CAACAATGCC
    ACAAGGACCA GAATTGTACA TCATATTTTA CAAAGAGTCA AATATGAAGA GGGGAAAAAT
    AAAATCGGAC TGAACCGATT GCTCACAAAC TGCACATATG AAGCTGCCTT CCCCCTACAT
    GAGGGGAGTT ACAGAAGCAA AAATTCAATA AAGACACATG GAGCAGAAAA TCACAGACAT
    TTGCTTTTTG AATGTTGGGC CTCCTGGGGA GTATGGTATA AATACCAACC ACTAGATCTG
    GTCAGGCGGT ATTTCGGAGA GAAGATAGGT CTGTATTTTG CATGGCTTGG CTGGTACACA
    GGAATGCTTT TCCCAGCAGC CATTGTTGGC TTGTTTGTTT TCCTCTATGG AATGTTTACA
    CTAGACAGCT GCCAAGTCAG TCGAGAAATC TGCCAAGCCA CAAACATTAC TATGTGTCCA
    CTATGTGACA AATATTGTCC ATACATGAGG TTGTCAGACA GTTGCATCTA TGCCAAGGTT
    ACCCATCTGT TTGACAACAG TGCAACAGTA TTCTTTGCTG TTTTTATGGC AGTTTGGGCC
    ACAGTGTTTC TGGAGTTTTG GAAAAGAAGA AGAGCAGTAA TTGCTTATGA CTGGGACTTA
    ATAGACTGGG AAGAAGAAGA GGAAGAAATC CGCCCTCAGT TTGAAGCCAA ATACTCAAAG
    AAAGAGCGTG TAAACCCAAT ATCAGGAAAG CCAGAGCCTT ATCAAGCCTT TGCAGATAAA
    TGCAGCAGAC TTGTTGTTTC TGCTTCTGGA ATATTTTTTA TGATATGTGT CGTAATTGCT
    GCAGTTTTCG GCATTGTTAT TTATCGTGTT GTGACTGTCA GTACGTTTGC TGCCTTTAAG
    TGGGCGTTAA TAAGGAACAA TTCTCAGGTT GCTACTACAG GGACGGCGGT GTGCATAAAC
    TTTTGCATTA TAATGCTTCT GAATGTGCTC TATGAAAGGG TTGCACTCTT CCTTACAAAC
    CTAGAACAGC CTCGCACTGA ATCAGAATGG GAGAACAGCT TTACTTTGAA AATGTTTCTT
    TTTCAGTTTG TCAATCTGAA CAGTTCCACC TTTTACATTG CATTCTTTCT TGGGCGGTTT
    ACTGGCAGAC CAGGGGCTTA TTTAAGGCTC ATAAACAAAT GGCGACTTGA AGAGTGTCAC
    CCAAGTGGAT GCCTTATTGA CCTATGCCTG CAGATGGGAA TAATTATGGT TCTCAAACAG
    ACCTGGAATA ACTTCATGGA ACTTGGACTT ATTCAGAACT GGTGGACTCG AAGAAAATTA
    CGTCAAGAGC ATGGAATTCA TGGAAAAACT GCCCTTCCAC AGTGGGAAAA GGACTACAAC
    TTGCAGCCAA TCAATCCATA TGGACTCTTT GAAGAGTATC TGGAAATGAT TCTCCAGTTT
    GGCTTCACAA CCATCTTTGT GGCTGCCTTT CCATTAGCTC CTCTACTTGC CCTACTTAAC
    AACATCATTG AAATCCGACT TGATGCATAT AAATTCATCA CTCAGTGGAG GCGGCCTCTA
    GCTTCCCGGG CAAAGGATAT AGGCATATGG TATGGAATTC TTGAAGGCAT CGGAATTCTC
    TCAGTTATCA CCAACGCTTT TGTCATAGCT GTGACCTCGG ACTTCATCCC TCGTCTGGTC
    TACGCTTACA AATATGGACC CTGTGCAGGT CAAGGACGCG CTGGAGAAAA GTGCATGGTC
    GGTTATGTTA ATGCCAGTCT TTCAGTATTT TTGGTGACTG ATTTTGAAAA CCATTCAAAT
    TCTTCACGCA ATGGAAGAGA ATTTGCTGAA CAATATGTAA AATATTGCAG GTACCGTGAC
    TATAGAGACC CCCCAAGCGC TCCTGAGCCC TACGCTTATA CACTGCAGTT CTGGCATGTT
    TTAGCAGCTC GATTGGCATT CATTATTGTA TTTGAGCATC TTGTCTTTTT CATAAAGCAT
    CTGATCTCTT ACCTAATACC AGACCTTCCC AAGGACCTAA GGGACAGGAT GAGAAGAGAA
    AAATACCTTA TACAGGAGAT GATGTATGAA GCAGAGCTAG AGAGAGTCCA AAAAGAAAAG
    AAAGAAAGAA AAAAAAATGG AAAATGTCAA CATAATGAGT GGCCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.