vwce cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	vwce
Entrez Gene ID	100488202
Full Name	von Willebrand factor C and EGF domains
General protein information	Preferred Names von Willebrand factor C and EGF domains Names von Willebrand factor C and EGF domain-containing protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018093327.1	XP_017948816.1	von Willebrand factor C and EGF domain-containing protein isoform X3
XM_031900559.1	XP_031756419.1	von Willebrand factor C and EGF domain-containing protein isoform X5
XM_018093326.1	XP_017948815.1	von Willebrand factor C and EGF domain-containing protein isoform X2
XM_018093326.2	XP_017948815.2	von Willebrand factor C and EGF domain-containing protein isoform X2
XM_018093325.1	XP_017948814.1	von Willebrand factor C and EGF domain-containing protein isoform X1
XM_018093325.2	XP_017948814.2	von Willebrand factor C and EGF domain-containing protein isoform X1
XM_018093327.2	XP_017948816.2	von Willebrand factor C and EGF domain-containing protein isoform X3
XM_031900558.1	XP_031756418.1	von Willebrand factor C and EGF domain-containing protein isoform X4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Vwce	NP_082189.1
Pan troglodytes (chimpanzee)	VWCE	XP_003313116.2
Xenopus tropicalis (tropical clawed frog)	LOC100488202	XP_002937703.2
Canis lupus familiaris (dog)	VWCE	XP_005631743.1
Bos taurus (cattle)	VWCE	NP_001178138.1
Homo sapiens (human)	VWCE	NP_689931.2
	VWCE	XP_001083081.2
Rattus norvegicus (Norway rat)	Vwce	NP_001258240.1

Gene Ontology

The following vwce gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vwce cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa44000	XM_018093325.1 Latest version!	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa44001	XM_018093326.1 Latest version!	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa44002	XM_018093327.1 Latest version!	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa56582	XM_018093325.2 Latest version!	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56583	XM_018093326.2 Latest version!	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56584	XM_018093327.2 Latest version!	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56585	XM_031900558.1 Latest version!	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa56586	XM_031900559.1 Latest version!	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vwce ( XM_018093325.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948814.1 Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa44000
Clone ID Related Accession (Same CDS sequence)	XM_018093325.1
Accession Version	XM_018093325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor C and EGF domain-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTAC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCTAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATTGAT GAATGTTCCT CTAAAAGATC CCTGTGCCAA 
CAAAAATGCA AGAACCTTCA GGGCAGCTAC AGGTGTTTGT GTGCGACGGG GTTCGTCCTT 
CAGAGCAATG GACACACCTG TGCAGACATA AATGAATGTC GGAGGCCAGG AACATCCCAT 
CTTTGCAAAC ACTTCTGCCA CAATACTCAT GGAAGTTTCT TCTGTTCTTG CCGGGCTGGG 
TTTCTGCTTG ATCCAGATAA GGTGTCATGT ATTGATGTGG ATGAATGTTT AGAAAATGCC 
ACCATATGCC CTCTGGGAAG CTGTGTGAAC ACACTGGGGT CCTATTTCTG TGCAGAGATT 
CCAGCGGGAA TGCAGACCAC AGGCCTCCCT TGGGAAACAC AGGCAGCACA TGGGAACTGG 
ATGCCATTCC AGCCAACATC AGTAATGCCC CAGACATCTG AGTCACTGAC TACTGGTGGC 
CCTCACCCTC TCAGGACCAG CACTGAGTTA GCACAAGGCA TTTTCAGCAC AACTACTACC 
TCAGCACCAC AAAGCACTTT GGCAAACACA AACATCATAC CACGTCCACA GCACCTCAGA 
ATTGGCCACC CTCCACTAGC TCAGAAAGCC TCTGGAATTT CCCATATGCC TCTGAAAGAA 
AACATGATAG CCCAATCAGG TACCACCACT GTATCATTCA CTAACGTGCC AGATTCCCAC 
TGCTGGCATA ACAAGGAGCT GCATTTAAGT GGTAGTACAT GGGCAGCACT GGGATGTACT 
GACTGCACTT GCCAGGATGG AAATATATCT TGTGAAAAAA GGATATGCAC CCCAAACTGT 
TCACACCCCG TCATGTATCC TGACAGCTGC TGCCCTATGT GTGACGGATG CCTTTTTGAG 
GGAGTTTCCA GGGTAGATGG AGAGCTTTTC CCAAATTCTC CAGACAATTG CACAATCTGC 
ATCTGCCTGG CTGGAAATGT CACATGTATC CCTCCTGTGT GCCCTCCTGT TACATGCACT 
GACCCCTTCC TATCAGACTG CTGCCTCAGA TGCCCAGATG GATGTGAATT CCAAGATCAT 
TTTTATCCCC ATGGATCCAA ATTTAGCAGG GATGATAATG GATGCACCTC TTGTTTATGT 
CAGAACGGCG CTGTTGAATG TTCGTTTATT CCATGTCCCA GTCTAGAATG TCCCAGAGAG 
AACTGGGTGC TGGAGGCCGG CCAGTGTTGC TTTAAATGCC AGGAACCCAT GCAAAGGACA 
GGGTGTCCTT TTGATGATAA TGGAATTGAA ATTCCTGTTG GGCAGATCTG GTCGCCGGGA 
GATCCCTGTG CAATCTGCAT TTGTCAGGCA GACAGCTCCA TCGTGTGCAA GAAAACAGAC 
TGCGTGGAGA CCTGTCCACA TCCAATCACA GTGCCAGGGC AATGCTGCCC AGATTGCTCA 
GCGGGTTGTT CTTATGGTAG AAGAACATTC AGGAACAATG AAAGCTTCCC CTCTAATTCA 
GACCCATGTC TCACTTGCAT TTGTCTGATG GGCACTGTAG CCTGTTCTCC AATTGAGTGT 
GCACTAAACT GCACGTACCC TTTCCACGAT GAAGGAGAGT GCTGCCCTGT TTGTCGAGAT 
TGCACATATG ATGGCAGAAA AGTTCTGAAT GGACAGACTT TCTACCTCGA GAGTGATCCT 
TGCACTCAGT GCACTTGTCA GCTTGGAGAG GTGCATTGTG AGGCAATCGT GTGCTCTGCT 
TCCTGCTCAC ATCCTTACGT CTTTCCTGGG GAATGTTGCT CCTCTTGTGA AGAGTGTCTC 
TTTGAAGGCC ATCTTTTAGA GAATGGAGGC TCCTACATAT TAAAGGTGGA TCCCTGCATT 
GTGTGTCATT GTTCTGGTGG AAATGTTCAA TGTGAGCAAA GGGATAATTC CTGTACTCCA 
TGTGTGCAGA AGACACAGGA TTGTCTAAAT GAAGTGCCAG ATATTTTTCA TTATAATCAG 
CTGCAAATCA TGCCTGCGGA TCTACATCAG TCTGCTGGAT CCCACAGTCC AAATATCGCA 
CTGAAGCTAC TGAATGATGC AGTGATTTCC CAACCCACCT ATATTCCTTT TTTCCAGAGA 
TTAGTATCCA GGCGAAATAC TGAGATCTTA GGAACAATTT CAGCTGGTCC AACTACTGTC 
CTTCATGGAA AAACACCTGC AGACTCAACT CCTCCTGGCT ATAATAAGGA TTATTTAACT 
ACAAACCCTG TTGACCCTTT GCCAAATGCA CACACAACAA TCACCCACAC AGGAGAAATA 
AAGTCTGTTT CAGCATCAGA GCCATTCAGA AACATTATTA TGCCTTCTTC AAAATTGCCT 
CAAAGAGAAG CTACATCATC AAACTCATCT CCACAACCTG AAGAATATTT GCATGAATCT 
TTTCTGGGTT TGCATACACA AAAGTTATCT ACACCTACCT CCTCTTTAGG CATTCCTAAT 
AAGTCTTTCC CTTCAGCAGT TATTTCTACC TCAACACTAA TGCCTCAAAT GTTTCCTTTT 
TCTGATATGT CTTCTGGTCC ATCCAAACTA ACTTCTGACA GCGCTGCCCT TCATCCACCT 
GCTTGGAATG CAGATAGTTC TGTTCAGTCA CCAGCACATG ATTTTATTTC TTCTTTCACC 
CATACACCGG ACTCACATTC TTTATTTACC CACTCACAAA GACCCCTCTC TTCTGGCACT 
TCTGATATAT CATCTATCCA TGCAGATACC ACTCAATTCT CTTTGTTAGC CAAAAACACT 
CTAGGTTATA CCTCGGCCCC TGCACCCCAT GCTGCTCACT TCCCAACCTC TGCAAACATC 
ATCCCGAGTG ATTCTGGGAT TCCTCTCATG CAGCTAAACC ACAGAGAGTC AGAGAGTGAT 
GAACTGGGGA ATGGACACGC AATTGGATGT TCTTTAAAAG GTCTTGAATT TTCCAGTGGC 
TCCATCTTTG TTGCTGACAT GGACTACTGC CTGCCCTGTG AATGTTTGAA TGGAGAGATC 
TACTGTTCAC ACCAAGATTT ATTCCCCAGT GGACTGTGTT GCCAGAGCTG TAGCCACCAG 
GGTGTTGATT CTTGTGCAGG GAAAATAAAC CAGGTCCATT CAGAAAGTAT GAGGAGTCAG 
AACCTTTGCA ATTGCAGAAA TCTCAGTCTT CACTGTGACT CTTGCTCGTT CAGAGAAGAA 
TGCAGAAGAA ATGGCTCTAC ACAAACCGCC AAGGAAGAAC AACTACCTGA GAACATCCAC 
TGGGCTCCAC TTATATTTGA AGGGGATGTT GAGCCTCAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948814.1
CDS	618..4742
Translation	MLPFWLGMSCLLLLISETGGRVYKGRKKPPALTAQRLRSGPHVCFSGMGAGCCPGWTVSPGTGQCLQPLCSFGCASGFCIAPNVCSCRDGHQGVTCADEFSSEHEKTLEDKGTPLTCLSALCDQNCRLVNGVPSCSCFHGYSLGKDGRTCYDVDECSRPQASSLCQQQCKNSIGSYRCLCFYGYQISPNGRSCIPTKNLNFIAGIAAPSPCGEYGCELMCNDGGCEHVSRVCPVGFRMAETVNGVTCTDIDECEASSCEGTCINTEGGFVCDCGPGMKLSTDRTSCYDIDECSSKRSLCQQKCKNLQGSYRCLCATGFVLQSNGHTCADINECRRPGTSHLCKHFCHNTHGSFFCSCRAGFLLDPDKVSCIDVDECLENATICPLGSCVNTLGSYFCAEIPAGMQTTGLPWETQAAHGNWMPFQPTSVMPQTSESLTTGGPHPLRTSTELAQGIFSTTTTSAPQSTLANTNIIPRPQHLRIGHPPLAQKASGISHMPLKENMIAQSGTTTVSFTNVPDSHCWHNKELHLSGSTWAALGCTDCTCQDGNISCEKRICTPNCSHPVMYPDSCCPMCDGCLFEGVSRVDGELFPNSPDNCTICICLAGNVTCIPPVCPPVTCTDPFLSDCCLRCPDGCEFQDHFYPHGSKFSRDDNGCTSCLCQNGAVECSFIPCPSLECPRENWVLEAGQCCFKCQEPMQRTGCPFDDNGIEIPVGQIWSPGDPCAICICQADSSIVCKKTDCVETCPHPITVPGQCCPDCSAGCSYGRRTFRNNESFPSNSDPCLTCICLMGTVACSPIECALNCTYPFHDEGECCPVCRDCTYDGRKVLNGQTFYLESDPCTQCTCQLGEVHCEAIVCSASCSHPYVFPGECCSSCEECLFEGHLLENGGSYILKVDPCIVCHCSGGNVQCEQRDNSCTPCVQKTQDCLNEVPDIFHYNQLQIMPADLHQSAGSHSPNIALKLLNDAVISQPTYIPFFQRLVSRRNTEILGTISAGPTTVLHGKTPADSTPPGYNKDYLTTNPVDPLPNAHTTITHTGEIKSVSASEPFRNIIMPSSKLPQREATSSNSSPQPEEYLHESFLGLHTQKLSTPTSSLGIPNKSFPSAVISTSTLMPQMFPFSDMSSGPSKLTSDSAALHPPAWNADSSVQSPAHDFISSFTHTPDSHSLFTHSQRPLSSGTSDISSIHADTTQFSLLAKNTLGYTSAPAPHAAHFPTSANIIPSDSGIPLMQLNHRESESDELGNGHAIGCSLKGLEFSSGSIFVADMDYCLPCECLNGEIYCSHQDLFPSGLCCQSCSHQGVDSCAGKINQVHSESMRSQNLCNCRNLSLHCDSCSFREECRRNGSTQTAKEEQLPENIHWAPLIFEGDVEPQR

Target ORF information:

RefSeq Version	XM_018093325.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093325.1

ORF Insert Sequence:

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTAC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCTAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATTGAT GAATGTTCCT CTAAAAGATC CCTGTGCCAA 
CAAAAATGCA AGAACCTTCA GGGCAGCTAC AGGTGTTTGT GTGCGACGGG GTTCGTCCTT 
CAGAGCAATG GACACACCTG TGCAGACATA AATGAATGTC GGAGGCCAGG AACATCCCAT 
CTTTGCAAAC ACTTCTGCCA CAATACTCAT GGAAGTTTCT TCTGTTCTTG CCGGGCTGGG 
TTTCTGCTTG ATCCAGATAA GGTGTCATGT ATTGATGTGG ATGAATGTTT AGAAAATGCC 
ACCATATGCC CTCTGGGAAG CTGTGTGAAC ACACTGGGGT CCTATTTCTG TGCAGAGATT 
CCAGCGGGAA TGCAGACCAC AGGCCTCCCT TGGGAAACAC AGGCAGCACA TGGGAACTGG 
ATGCCATTCC AGCCAACATC AGTAATGCCC CAGACATCTG AGTCACTGAC TACTGGTGGC 
CCTCACCCTC TCAGGACCAG CACTGAGTTA GCACAAGGCA TTTTCAGCAC AACTACTACC 
TCAGCACCAC AAAGCACTTT GGCAAACACA AACATCATAC CACGTCCACA GCACCTCAGA 
ATTGGCCACC CTCCACTAGC TCAGAAAGCC TCTGGAATTT CCCATATGCC TCTGAAAGAA 
AACATGATAG CCCAATCAGG TACCACCACT GTATCATTCA CTAACGTGCC AGATTCCCAC 
TGCTGGCATA ACAAGGAGCT GCATTTAAGT GGTAGTACAT GGGCAGCACT GGGATGTACT 
GACTGCACTT GCCAGGATGG AAATATATCT TGTGAAAAAA GGATATGCAC CCCAAACTGT 
TCACACCCCG TCATGTATCC TGACAGCTGC TGCCCTATGT GTGACGGATG CCTTTTTGAG 
GGAGTTTCCA GGGTAGATGG AGAGCTTTTC CCAAATTCTC CAGACAATTG CACAATCTGC 
ATCTGCCTGG CTGGAAATGT CACATGTATC CCTCCTGTGT GCCCTCCTGT TACATGCACT 
GACCCCTTCC TATCAGACTG CTGCCTCAGA TGCCCAGATG GATGTGAATT CCAAGATCAT 
TTTTATCCCC ATGGATCCAA ATTTAGCAGG GATGATAATG GATGCACCTC TTGTTTATGT 
CAGAACGGCG CTGTTGAATG TTCGTTTATT CCATGTCCCA GTCTAGAATG TCCCAGAGAG 
AACTGGGTGC TGGAGGCCGG CCAGTGTTGC TTTAAATGCC AGGAACCCAT GCAAAGGACA 
GGGTGTCCTT TTGATGATAA TGGAATTGAA ATTCCTGTTG GGCAGATCTG GTCGCCGGGA 
GATCCCTGTG CAATCTGCAT TTGTCAGGCA GACAGCTCCA TCGTGTGCAA GAAAACAGAC 
TGCGTGGAGA CCTGTCCACA TCCAATCACA GTGCCAGGGC AATGCTGCCC AGATTGCTCA 
GCGGGTTGTT CTTATGGTAG AAGAACATTC AGGAACAATG AAAGCTTCCC CTCTAATTCA 
GACCCATGTC TCACTTGCAT TTGTCTGATG GGCACTGTAG CCTGTTCTCC AATTGAGTGT 
GCACTAAACT GCACGTACCC TTTCCACGAT GAAGGAGAGT GCTGCCCTGT TTGTCGAGAT 
TGCACATATG ATGGCAGAAA AGTTCTGAAT GGACAGACTT TCTACCTCGA GAGTGATCCT 
TGCACTCAGT GCACTTGTCA GCTTGGAGAG GTGCATTGTG AGGCAATCGT GTGCTCTGCT 
TCCTGCTCAC ATCCTTACGT CTTTCCTGGG GAATGTTGCT CCTCTTGTGA AGAGTGTCTC 
TTTGAAGGCC ATCTTTTAGA GAATGGAGGC TCCTACATAT TAAAGGTGGA TCCCTGCATT 
GTGTGTCATT GTTCTGGTGG AAATGTTCAA TGTGAGCAAA GGGATAATTC CTGTACTCCA 
TGTGTGCAGA AGACACAGGA TTGTCTAAAT GAAGTGCCAG ATATTTTTCA TTATAATCAG 
CTGCAAATCA TGCCTGCGGA TCTACATCAG TCTGCTGGAT CCCACAGTCC AAATATCGCA 
CTGAAGCTAC TGAATGATGC AGTGATTTCC CAACCCACCT ATATTCCTTT TTTCCAGAGA 
TTAGTATCCA GGCGAAATAC TGAGATCTTA GGAACAATTT CAGCTGGTCC AACTACTGTC 
CTTCATGGAA AAACACCTGC AGACTCAACT CCTCCTGGCT ATAATAAGGA TTATTTAACT 
ACAAACCCTG TTGACCCTTT GCCAAATGCA CACACAACAA TCACCCACAC AGGAGAAATA 
AAGTCTGTTT CAGCATCAGA GCCATTCAGA AACATTATTA TGCCTTCTTC AAAATTGCCT 
CAAAGAGAAG CTACATCATC AAACTCATCT CCACAACCTG AAGAATATTT GCATGAATCT 
TTTCTGGGTT TGCATACACA AAAGTTATCT ACACCTACCT CCTCTTTAGG CATTCCTAAT 
AAGTCTTTCC CTTCAGCAGT TATTTCTACC TCAACACTAA TGCCTCAAAT GTTTCCTTTT 
TCTGATATGT CTTCTGGTCC ATCCAAACTA ACTTCTGACA GCGCTGCCCT TCATCCACCT 
GCTTGGAATG CAGATAGTTC TGTTCAGTCA CCAGCACATG ATTTTATTTC TTCTTTCACC 
CATACACCGG ACTCACATTC TTTATTTACC CACTCACAAA GACCCCTCTC TTCTGGCACT 
TCTGATATAT CATCTATCCA TGCAGATACC ACTCAATTCT CTTTGTTAGC CAAAAACACT 
CTAGGTTATA CCTCGGCCCC TGCACCCCAT GCTGCTCACT TCCCAACCTC TGCAAACATC 
ATCCCGAGTG ATTCTGGGAT TCCTCTCATG CAGCTAAACC ACAGAGAGTC AGAGAGTGAT 
GAACTGGGGA ATGGACACGC AATTGGATGT TCTTTAAAAG GTCTTGAATT TTCCAGTGGC 
TCCATCTTTG TTGCTGACAT GGACTACTGC CTGCCCTGTG AATGTTTGAA TGGAGAGATC 
TACTGTTCAC ACCAAGATTT ATTCCCCAGT GGACTGTGTT GCCAGAGCTG TAGCCACCAG 
GGTGTTGATT CTTGTGCAGG GAAAATAAAC CAGGTCCATT CAGAAAGTAT GAGGAGTCAG 
AACCTTTGCA ATTGCAGAAA TCTCAGTCTT CACTGTGACT CTTGCTCGTT CAGAGAAGAA 
TGCAGAAGAA ATGGCTCTAC ACAAACCGCC AAGGAAGAAC AACTACCTGA GAACATCCAC 
TGGGCTCCAC TTATATTTGA AGGGGATGTT GAGCCTCAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwce ( XM_018093326.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948815.1 Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa44001
Clone ID Related Accession (Same CDS sequence)	XM_018093326.1
Accession Version	XM_018093326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor C and EGF domain-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTAC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCTAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATTGAT GAATGTTCCT CTAAAAGATC CCTGTGCCAA 
CAAAAATGCA AGAACCTTCA GGGCAGCTAC AGGTGTTTGT GTGCGACGGG GTTCGTCCTT 
CAGAGCAATG GACACACCTG TGCAGACATA AATGAATGTC GGAGGCCAGG AACATCCCAT 
CTTTGCAAAC ACTTCTGCCA CAATACTCAT GGAAGTTTCT TCTGTTCTTG CCGGGCTGGG 
TTTCTGCTTG ATCCAGATAA GGTGTCATGT ATTGAGATTC CAGCGGGAAT GCAGACCACA 
GGCCTCCCTT GGGAAACACA GGCAGCACAT GGGAACTGGA TGCCATTCCA GCCAACATCA 
GTAATGCCCC AGACATCTGA GTCACTGACT ACTGGTGGCC CTCACCCTCT CAGGACCAGC 
ACTGAGTTAG CACAAGGCAT TTTCAGCACA ACTACTACCT CAGCACCACA AAGCACTTTG 
GCAAACACAA ACATCATACC ACGTCCACAG CACCTCAGAA TTGGCCACCC TCCACTAGCT 
CAGAAAGCCT CTGGAATTTC CCATATGCCT CTGAAAGAAA ACATGATAGC CCAATCAGGT 
ACCACCACTG TATCATTCAC TAACGTGCCA GATTCCCACT GCTGGCATAA CAAGGAGCTG 
CATTTAAGTG GTAGTACATG GGCAGCACTG GGATGTACTG ACTGCACTTG CCAGGATGGA 
AATATATCTT GTGAAAAAAG GATATGCACC CCAAACTGTT CACACCCCGT CATGTATCCT 
GACAGCTGCT GCCCTATGTG TGACGGATGC CTTTTTGAGG GAGTTTCCAG GGTAGATGGA 
GAGCTTTTCC CAAATTCTCC AGACAATTGC ACAATCTGCA TCTGCCTGGC TGGAAATGTC 
ACATGTATCC CTCCTGTGTG CCCTCCTGTT ACATGCACTG ACCCCTTCCT ATCAGACTGC 
TGCCTCAGAT GCCCAGATGG ATGTGAATTC CAAGATCATT TTTATCCCCA TGGATCCAAA 
TTTAGCAGGG ATGATAATGG ATGCACCTCT TGTTTATGTC AGAACGGCGC TGTTGAATGT 
TCGTTTATTC CATGTCCCAG TCTAGAATGT CCCAGAGAGA ACTGGGTGCT GGAGGCCGGC 
CAGTGTTGCT TTAAATGCCA GGAACCCATG CAAAGGACAG GGTGTCCTTT TGATGATAAT 
GGAATTGAAA TTCCTGTTGG GCAGATCTGG TCGCCGGGAG ATCCCTGTGC AATCTGCATT 
TGTCAGGCAG ACAGCTCCAT CGTGTGCAAG AAAACAGACT GCGTGGAGAC CTGTCCACAT 
CCAATCACAG TGCCAGGGCA ATGCTGCCCA GATTGCTCAG CGGGTTGTTC TTATGGTAGA 
AGAACATTCA GGAACAATGA AAGCTTCCCC TCTAATTCAG ACCCATGTCT CACTTGCATT 
TGTCTGATGG GCACTGTAGC CTGTTCTCCA ATTGAGTGTG CACTAAACTG CACGTACCCT 
TTCCACGATG AAGGAGAGTG CTGCCCTGTT TGTCGAGATT GCACATATGA TGGCAGAAAA 
GTTCTGAATG GACAGACTTT CTACCTCGAG AGTGATCCTT GCACTCAGTG CACTTGTCAG 
CTTGGAGAGG TGCATTGTGA GGCAATCGTG TGCTCTGCTT CCTGCTCACA TCCTTACGTC 
TTTCCTGGGG AATGTTGCTC CTCTTGTGAA GAGTGTCTCT TTGAAGGCCA TCTTTTAGAG 
AATGGAGGCT CCTACATATT AAAGGTGGAT CCCTGCATTG TGTGTCATTG TTCTGGTGGA 
AATGTTCAAT GTGAGCAAAG GGATAATTCC TGTACTCCAT GTGTGCAGAA GACACAGGAT 
TGTCTAAATG AAGTGCCAGA TATTTTTCAT TATAATCAGC TGCAAATCAT GCCTGCGGAT 
CTACATCAGT CTGCTGGATC CCACAGTCCA AATATCGCAC TGAAGCTACT GAATGATGCA 
GTGATTTCCC AACCCACCTA TATTCCTTTT TTCCAGAGAT TAGTATCCAG GCGAAATACT 
GAGATCTTAG GAACAATTTC AGCTGGTCCA ACTACTGTCC TTCATGGAAA AACACCTGCA 
GACTCAACTC CTCCTGGCTA TAATAAGGAT TATTTAACTA CAAACCCTGT TGACCCTTTG 
CCAAATGCAC ACACAACAAT CACCCACACA GGAGAAATAA AGTCTGTTTC AGCATCAGAG 
CCATTCAGAA ACATTATTAT GCCTTCTTCA AAATTGCCTC AAAGAGAAGC TACATCATCA 
AACTCATCTC CACAACCTGA AGAATATTTG CATGAATCTT TTCTGGGTTT GCATACACAA 
AAGTTATCTA CACCTACCTC CTCTTTAGGC ATTCCTAATA AGTCTTTCCC TTCAGCAGTT 
ATTTCTACCT CAACACTAAT GCCTCAAATG TTTCCTTTTT CTGATATGTC TTCTGGTCCA 
TCCAAACTAA CTTCTGACAG CGCTGCCCTT CATCCACCTG CTTGGAATGC AGATAGTTCT 
GTTCAGTCAC CAGCACATGA TTTTATTTCT TCTTTCACCC ATACACCGGA CTCACATTCT 
TTATTTACCC ACTCACAAAG ACCCCTCTCT TCTGGCACTT CTGATATATC ATCTATCCAT 
GCAGATACCA CTCAATTCTC TTTGTTAGCC AAAAACACTC TAGGTTATAC CTCGGCCCCT 
GCACCCCATG CTGCTCACTT CCCAACCTCT GCAAACATCA TCCCGAGTGA TTCTGGGATT 
CCTCTCATGC AGCTAAACCA CAGAGAGTCA GAGAGTGATG AACTGGGGAA TGGACACGCA 
ATTGGATGTT CTTTAAAAGG TCTTGAATTT TCCAGTGGCT CCATCTTTGT TGCTGACATG 
GACTACTGCC TGCCCTGTGA ATGTTTGAAT GGAGAGATCT ACTGTTCACA CCAAGATTTA 
TTCCCCAGTG GACTGTGTTG CCAGAGCTGT AGCCACCAGG GTGTTGATTC TTGTGCAGGG 
AAAATAAACC AGGTCCATTC AGAAAGTATG AGGAGTCAGA ACCTTTGCAA TTGCAGAAAT 
CTCAGTCTTC ACTGTGACTC TTGCTCGTTC AGAGAAGAAT GCAGAAGAAA TGGCTCTACA 
CAAACCGCCA AGGAAGAACA ACTACCTGAG AACATCCACT GGGCTCCACT TATATTTGAA 
GGGGATGTTG AGCCTCAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948815.1
CDS	618..4661
Translation	MLPFWLGMSCLLLLISETGGRVYKGRKKPPALTAQRLRSGPHVCFSGMGAGCCPGWTVSPGTGQCLQPLCSFGCASGFCIAPNVCSCRDGHQGVTCADEFSSEHEKTLEDKGTPLTCLSALCDQNCRLVNGVPSCSCFHGYSLGKDGRTCYDVDECSRPQASSLCQQQCKNSIGSYRCLCFYGYQISPNGRSCIPTKNLNFIAGIAAPSPCGEYGCELMCNDGGCEHVSRVCPVGFRMAETVNGVTCTDIDECEASSCEGTCINTEGGFVCDCGPGMKLSTDRTSCYDIDECSSKRSLCQQKCKNLQGSYRCLCATGFVLQSNGHTCADINECRRPGTSHLCKHFCHNTHGSFFCSCRAGFLLDPDKVSCIEIPAGMQTTGLPWETQAAHGNWMPFQPTSVMPQTSESLTTGGPHPLRTSTELAQGIFSTTTTSAPQSTLANTNIIPRPQHLRIGHPPLAQKASGISHMPLKENMIAQSGTTTVSFTNVPDSHCWHNKELHLSGSTWAALGCTDCTCQDGNISCEKRICTPNCSHPVMYPDSCCPMCDGCLFEGVSRVDGELFPNSPDNCTICICLAGNVTCIPPVCPPVTCTDPFLSDCCLRCPDGCEFQDHFYPHGSKFSRDDNGCTSCLCQNGAVECSFIPCPSLECPRENWVLEAGQCCFKCQEPMQRTGCPFDDNGIEIPVGQIWSPGDPCAICICQADSSIVCKKTDCVETCPHPITVPGQCCPDCSAGCSYGRRTFRNNESFPSNSDPCLTCICLMGTVACSPIECALNCTYPFHDEGECCPVCRDCTYDGRKVLNGQTFYLESDPCTQCTCQLGEVHCEAIVCSASCSHPYVFPGECCSSCEECLFEGHLLENGGSYILKVDPCIVCHCSGGNVQCEQRDNSCTPCVQKTQDCLNEVPDIFHYNQLQIMPADLHQSAGSHSPNIALKLLNDAVISQPTYIPFFQRLVSRRNTEILGTISAGPTTVLHGKTPADSTPPGYNKDYLTTNPVDPLPNAHTTITHTGEIKSVSASEPFRNIIMPSSKLPQREATSSNSSPQPEEYLHESFLGLHTQKLSTPTSSLGIPNKSFPSAVISTSTLMPQMFPFSDMSSGPSKLTSDSAALHPPAWNADSSVQSPAHDFISSFTHTPDSHSLFTHSQRPLSSGTSDISSIHADTTQFSLLAKNTLGYTSAPAPHAAHFPTSANIIPSDSGIPLMQLNHRESESDELGNGHAIGCSLKGLEFSSGSIFVADMDYCLPCECLNGEIYCSHQDLFPSGLCCQSCSHQGVDSCAGKINQVHSESMRSQNLCNCRNLSLHCDSCSFREECRRNGSTQTAKEEQLPENIHWAPLIFEGDVEPQR

Target ORF information:

RefSeq Version	XM_018093326.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093326.1

ORF Insert Sequence:

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTAC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCTAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATTGAT GAATGTTCCT CTAAAAGATC CCTGTGCCAA 
CAAAAATGCA AGAACCTTCA GGGCAGCTAC AGGTGTTTGT GTGCGACGGG GTTCGTCCTT 
CAGAGCAATG GACACACCTG TGCAGACATA AATGAATGTC GGAGGCCAGG AACATCCCAT 
CTTTGCAAAC ACTTCTGCCA CAATACTCAT GGAAGTTTCT TCTGTTCTTG CCGGGCTGGG 
TTTCTGCTTG ATCCAGATAA GGTGTCATGT ATTGAGATTC CAGCGGGAAT GCAGACCACA 
GGCCTCCCTT GGGAAACACA GGCAGCACAT GGGAACTGGA TGCCATTCCA GCCAACATCA 
GTAATGCCCC AGACATCTGA GTCACTGACT ACTGGTGGCC CTCACCCTCT CAGGACCAGC 
ACTGAGTTAG CACAAGGCAT TTTCAGCACA ACTACTACCT CAGCACCACA AAGCACTTTG 
GCAAACACAA ACATCATACC ACGTCCACAG CACCTCAGAA TTGGCCACCC TCCACTAGCT 
CAGAAAGCCT CTGGAATTTC CCATATGCCT CTGAAAGAAA ACATGATAGC CCAATCAGGT 
ACCACCACTG TATCATTCAC TAACGTGCCA GATTCCCACT GCTGGCATAA CAAGGAGCTG 
CATTTAAGTG GTAGTACATG GGCAGCACTG GGATGTACTG ACTGCACTTG CCAGGATGGA 
AATATATCTT GTGAAAAAAG GATATGCACC CCAAACTGTT CACACCCCGT CATGTATCCT 
GACAGCTGCT GCCCTATGTG TGACGGATGC CTTTTTGAGG GAGTTTCCAG GGTAGATGGA 
GAGCTTTTCC CAAATTCTCC AGACAATTGC ACAATCTGCA TCTGCCTGGC TGGAAATGTC 
ACATGTATCC CTCCTGTGTG CCCTCCTGTT ACATGCACTG ACCCCTTCCT ATCAGACTGC 
TGCCTCAGAT GCCCAGATGG ATGTGAATTC CAAGATCATT TTTATCCCCA TGGATCCAAA 
TTTAGCAGGG ATGATAATGG ATGCACCTCT TGTTTATGTC AGAACGGCGC TGTTGAATGT 
TCGTTTATTC CATGTCCCAG TCTAGAATGT CCCAGAGAGA ACTGGGTGCT GGAGGCCGGC 
CAGTGTTGCT TTAAATGCCA GGAACCCATG CAAAGGACAG GGTGTCCTTT TGATGATAAT 
GGAATTGAAA TTCCTGTTGG GCAGATCTGG TCGCCGGGAG ATCCCTGTGC AATCTGCATT 
TGTCAGGCAG ACAGCTCCAT CGTGTGCAAG AAAACAGACT GCGTGGAGAC CTGTCCACAT 
CCAATCACAG TGCCAGGGCA ATGCTGCCCA GATTGCTCAG CGGGTTGTTC TTATGGTAGA 
AGAACATTCA GGAACAATGA AAGCTTCCCC TCTAATTCAG ACCCATGTCT CACTTGCATT 
TGTCTGATGG GCACTGTAGC CTGTTCTCCA ATTGAGTGTG CACTAAACTG CACGTACCCT 
TTCCACGATG AAGGAGAGTG CTGCCCTGTT TGTCGAGATT GCACATATGA TGGCAGAAAA 
GTTCTGAATG GACAGACTTT CTACCTCGAG AGTGATCCTT GCACTCAGTG CACTTGTCAG 
CTTGGAGAGG TGCATTGTGA GGCAATCGTG TGCTCTGCTT CCTGCTCACA TCCTTACGTC 
TTTCCTGGGG AATGTTGCTC CTCTTGTGAA GAGTGTCTCT TTGAAGGCCA TCTTTTAGAG 
AATGGAGGCT CCTACATATT AAAGGTGGAT CCCTGCATTG TGTGTCATTG TTCTGGTGGA 
AATGTTCAAT GTGAGCAAAG GGATAATTCC TGTACTCCAT GTGTGCAGAA GACACAGGAT 
TGTCTAAATG AAGTGCCAGA TATTTTTCAT TATAATCAGC TGCAAATCAT GCCTGCGGAT 
CTACATCAGT CTGCTGGATC CCACAGTCCA AATATCGCAC TGAAGCTACT GAATGATGCA 
GTGATTTCCC AACCCACCTA TATTCCTTTT TTCCAGAGAT TAGTATCCAG GCGAAATACT 
GAGATCTTAG GAACAATTTC AGCTGGTCCA ACTACTGTCC TTCATGGAAA AACACCTGCA 
GACTCAACTC CTCCTGGCTA TAATAAGGAT TATTTAACTA CAAACCCTGT TGACCCTTTG 
CCAAATGCAC ACACAACAAT CACCCACACA GGAGAAATAA AGTCTGTTTC AGCATCAGAG 
CCATTCAGAA ACATTATTAT GCCTTCTTCA AAATTGCCTC AAAGAGAAGC TACATCATCA 
AACTCATCTC CACAACCTGA AGAATATTTG CATGAATCTT TTCTGGGTTT GCATACACAA 
AAGTTATCTA CACCTACCTC CTCTTTAGGC ATTCCTAATA AGTCTTTCCC TTCAGCAGTT 
ATTTCTACCT CAACACTAAT GCCTCAAATG TTTCCTTTTT CTGATATGTC TTCTGGTCCA 
TCCAAACTAA CTTCTGACAG CGCTGCCCTT CATCCACCTG CTTGGAATGC AGATAGTTCT 
GTTCAGTCAC CAGCACATGA TTTTATTTCT TCTTTCACCC ATACACCGGA CTCACATTCT 
TTATTTACCC ACTCACAAAG ACCCCTCTCT TCTGGCACTT CTGATATATC ATCTATCCAT 
GCAGATACCA CTCAATTCTC TTTGTTAGCC AAAAACACTC TAGGTTATAC CTCGGCCCCT 
GCACCCCATG CTGCTCACTT CCCAACCTCT GCAAACATCA TCCCGAGTGA TTCTGGGATT 
CCTCTCATGC AGCTAAACCA CAGAGAGTCA GAGAGTGATG AACTGGGGAA TGGACACGCA 
ATTGGATGTT CTTTAAAAGG TCTTGAATTT TCCAGTGGCT CCATCTTTGT TGCTGACATG 
GACTACTGCC TGCCCTGTGA ATGTTTGAAT GGAGAGATCT ACTGTTCACA CCAAGATTTA 
TTCCCCAGTG GACTGTGTTG CCAGAGCTGT AGCCACCAGG GTGTTGATTC TTGTGCAGGG 
AAAATAAACC AGGTCCATTC AGAAAGTATG AGGAGTCAGA ACCTTTGCAA TTGCAGAAAT 
CTCAGTCTTC ACTGTGACTC TTGCTCGTTC AGAGAAGAAT GCAGAAGAAA TGGCTCTACA 
CAAACCGCCA AGGAAGAACA ACTACCTGAG AACATCCACT GGGCTCCACT TATATTTGAA 
GGGGATGTTG AGCCTCAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwce ( XM_018093327.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948816.1 Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X3, mRNA.

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CloneID	OXa44002
Clone ID Related Accession (Same CDS sequence)	XM_018093327.1
Accession Version	XM_018093327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor C and EGF domain-containing protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTAC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCTAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATAAAT GAATGTCGGA GGCCAGGAAC ATCCCATCTT 
TGCAAACACT TCTGCCACAA TACTCATGGA AGTTTCTTCT GTTCTTGCCG GGCTGGGTTT 
CTGCTTGATC CAGATAAGGT GTCATGTATT GATGTGGATG AATGTTTAGA AAATGCCACC 
ATATGCCCTC TGGGAAGCTG TGTGAACACA CTGGGGTCCT ATTTCTGTGC AGAGATTCCA 
GCGGGAATGC AGACCACAGG CCTCCCTTGG GAAACACAGG CAGCACATGG GAACTGGATG 
CCATTCCAGC CAACATCAGT AATGCCCCAG ACATCTGAGT CACTGACTAC TGGTGGCCCT 
CACCCTCTCA GGACCAGCAC TGAGTTAGCA CAAGGCATTT TCAGCACAAC TACTACCTCA 
GCACCACAAA GCACTTTGGC AAACACAAAC ATCATACCAC GTCCACAGCA CCTCAGAATT 
GGCCACCCTC CACTAGCTCA GAAAGCCTCT GGAATTTCCC ATATGCCTCT GAAAGAAAAC 
ATGATAGCCC AATCAGGTAC CACCACTGTA TCATTCACTA ACGTGCCAGA TTCCCACTGC 
TGGCATAACA AGGAGCTGCA TTTAAGTGGT AGTACATGGG CAGCACTGGG ATGTACTGAC 
TGCACTTGCC AGGATGGAAA TATATCTTGT GAAAAAAGGA TATGCACCCC AAACTGTTCA 
CACCCCGTCA TGTATCCTGA CAGCTGCTGC CCTATGTGTG ACGGATGCCT TTTTGAGGGA 
GTTTCCAGGG TAGATGGAGA GCTTTTCCCA AATTCTCCAG ACAATTGCAC AATCTGCATC 
TGCCTGGCTG GAAATGTCAC ATGTATCCCT CCTGTGTGCC CTCCTGTTAC ATGCACTGAC 
CCCTTCCTAT CAGACTGCTG CCTCAGATGC CCAGATGGAT GTGAATTCCA AGATCATTTT 
TATCCCCATG GATCCAAATT TAGCAGGGAT GATAATGGAT GCACCTCTTG TTTATGTCAG 
AACGGCGCTG TTGAATGTTC GTTTATTCCA TGTCCCAGTC TAGAATGTCC CAGAGAGAAC 
TGGGTGCTGG AGGCCGGCCA GTGTTGCTTT AAATGCCAGG AACCCATGCA AAGGACAGGG 
TGTCCTTTTG ATGATAATGG AATTGAAATT CCTGTTGGGC AGATCTGGTC GCCGGGAGAT 
CCCTGTGCAA TCTGCATTTG TCAGGCAGAC AGCTCCATCG TGTGCAAGAA AACAGACTGC 
GTGGAGACCT GTCCACATCC AATCACAGTG CCAGGGCAAT GCTGCCCAGA TTGCTCAGCG 
GGTTGTTCTT ATGGTAGAAG AACATTCAGG AACAATGAAA GCTTCCCCTC TAATTCAGAC 
CCATGTCTCA CTTGCATTTG TCTGATGGGC ACTGTAGCCT GTTCTCCAAT TGAGTGTGCA 
CTAAACTGCA CGTACCCTTT CCACGATGAA GGAGAGTGCT GCCCTGTTTG TCGAGATTGC 
ACATATGATG GCAGAAAAGT TCTGAATGGA CAGACTTTCT ACCTCGAGAG TGATCCTTGC 
ACTCAGTGCA CTTGTCAGCT TGGAGAGGTG CATTGTGAGG CAATCGTGTG CTCTGCTTCC 
TGCTCACATC CTTACGTCTT TCCTGGGGAA TGTTGCTCCT CTTGTGAAGA GTGTCTCTTT 
GAAGGCCATC TTTTAGAGAA TGGAGGCTCC TACATATTAA AGGTGGATCC CTGCATTGTG 
TGTCATTGTT CTGGTGGAAA TGTTCAATGT GAGCAAAGGG ATAATTCCTG TACTCCATGT 
GTGCAGAAGA CACAGGATTG TCTAAATGAA GTGCCAGATA TTTTTCATTA TAATCAGCTG 
CAAATCATGC CTGCGGATCT ACATCAGTCT GCTGGATCCC ACAGTCCAAA TATCGCACTG 
AAGCTACTGA ATGATGCAGT GATTTCCCAA CCCACCTATA TTCCTTTTTT CCAGAGATTA 
GTATCCAGGC GAAATACTGA GATCTTAGGA ACAATTTCAG CTGGTCCAAC TACTGTCCTT 
CATGGAAAAA CACCTGCAGA CTCAACTCCT CCTGGCTATA ATAAGGATTA TTTAACTACA 
AACCCTGTTG ACCCTTTGCC AAATGCACAC ACAACAATCA CCCACACAGG AGAAATAAAG 
TCTGTTTCAG CATCAGAGCC ATTCAGAAAC ATTATTATGC CTTCTTCAAA ATTGCCTCAA 
AGAGAAGCTA CATCATCAAA CTCATCTCCA CAACCTGAAG AATATTTGCA TGAATCTTTT 
CTGGGTTTGC ATACACAAAA GTTATCTACA CCTACCTCCT CTTTAGGCAT TCCTAATAAG 
TCTTTCCCTT CAGCAGTTAT TTCTACCTCA ACACTAATGC CTCAAATGTT TCCTTTTTCT 
GATATGTCTT CTGGTCCATC CAAACTAACT TCTGACAGCG CTGCCCTTCA TCCACCTGCT 
TGGAATGCAG ATAGTTCTGT TCAGTCACCA GCACATGATT TTATTTCTTC TTTCACCCAT 
ACACCGGACT CACATTCTTT ATTTACCCAC TCACAAAGAC CCCTCTCTTC TGGCACTTCT 
GATATATCAT CTATCCATGC AGATACCACT CAATTCTCTT TGTTAGCCAA AAACACTCTA 
GGTTATACCT CGGCCCCTGC ACCCCATGCT GCTCACTTCC CAACCTCTGC AAACATCATC 
CCGAGTGATT CTGGGATTCC TCTCATGCAG CTAAACCACA GAGAGTCAGA GAGTGATGAA 
CTGGGGAATG GACACGCAAT TGGATGTTCT TTAAAAGGTC TTGAATTTTC CAGTGGCTCC 
ATCTTTGTTG CTGACATGGA CTACTGCCTG CCCTGTGAAT GTTTGAATGG AGAGATCTAC 
TGTTCACACC AAGATTTATT CCCCAGTGGA CTGTGTTGCC AGAGCTGTAG CCACCAGGGT 
GTTGATTCTT GTGCAGGGAA AATAAACCAG GTCCATTCAG AAAGTATGAG GAGTCAGAAC 
CTTTGCAATT GCAGAAATCT CAGTCTTCAC TGTGACTCTT GCTCGTTCAG AGAAGAATGC 
AGAAGAAATG GCTCTACACA AACCGCCAAG GAAGAACAAC TACCTGAGAA CATCCACTGG 
GCTCCACTTA TATTTGAAGG GGATGTTGAG CCTCAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948816.1
CDS	618..4619
Translation	MLPFWLGMSCLLLLISETGGRVYKGRKKPPALTAQRLRSGPHVCFSGMGAGCCPGWTVSPGTGQCLQPLCSFGCASGFCIAPNVCSCRDGHQGVTCADEFSSEHEKTLEDKGTPLTCLSALCDQNCRLVNGVPSCSCFHGYSLGKDGRTCYDVDECSRPQASSLCQQQCKNSIGSYRCLCFYGYQISPNGRSCIPTKNLNFIAGIAAPSPCGEYGCELMCNDGGCEHVSRVCPVGFRMAETVNGVTCTDIDECEASSCEGTCINTEGGFVCDCGPGMKLSTDRTSCYDINECRRPGTSHLCKHFCHNTHGSFFCSCRAGFLLDPDKVSCIDVDECLENATICPLGSCVNTLGSYFCAEIPAGMQTTGLPWETQAAHGNWMPFQPTSVMPQTSESLTTGGPHPLRTSTELAQGIFSTTTTSAPQSTLANTNIIPRPQHLRIGHPPLAQKASGISHMPLKENMIAQSGTTTVSFTNVPDSHCWHNKELHLSGSTWAALGCTDCTCQDGNISCEKRICTPNCSHPVMYPDSCCPMCDGCLFEGVSRVDGELFPNSPDNCTICICLAGNVTCIPPVCPPVTCTDPFLSDCCLRCPDGCEFQDHFYPHGSKFSRDDNGCTSCLCQNGAVECSFIPCPSLECPRENWVLEAGQCCFKCQEPMQRTGCPFDDNGIEIPVGQIWSPGDPCAICICQADSSIVCKKTDCVETCPHPITVPGQCCPDCSAGCSYGRRTFRNNESFPSNSDPCLTCICLMGTVACSPIECALNCTYPFHDEGECCPVCRDCTYDGRKVLNGQTFYLESDPCTQCTCQLGEVHCEAIVCSASCSHPYVFPGECCSSCEECLFEGHLLENGGSYILKVDPCIVCHCSGGNVQCEQRDNSCTPCVQKTQDCLNEVPDIFHYNQLQIMPADLHQSAGSHSPNIALKLLNDAVISQPTYIPFFQRLVSRRNTEILGTISAGPTTVLHGKTPADSTPPGYNKDYLTTNPVDPLPNAHTTITHTGEIKSVSASEPFRNIIMPSSKLPQREATSSNSSPQPEEYLHESFLGLHTQKLSTPTSSLGIPNKSFPSAVISTSTLMPQMFPFSDMSSGPSKLTSDSAALHPPAWNADSSVQSPAHDFISSFTHTPDSHSLFTHSQRPLSSGTSDISSIHADTTQFSLLAKNTLGYTSAPAPHAAHFPTSANIIPSDSGIPLMQLNHRESESDELGNGHAIGCSLKGLEFSSGSIFVADMDYCLPCECLNGEIYCSHQDLFPSGLCCQSCSHQGVDSCAGKINQVHSESMRSQNLCNCRNLSLHCDSCSFREECRRNGSTQTAKEEQLPENIHWAPLIFEGDVEPQR

Target ORF information:

RefSeq Version	XM_018093327.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093327.1

ORF Insert Sequence:

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTAC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCTAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATAAAT GAATGTCGGA GGCCAGGAAC ATCCCATCTT 
TGCAAACACT TCTGCCACAA TACTCATGGA AGTTTCTTCT GTTCTTGCCG GGCTGGGTTT 
CTGCTTGATC CAGATAAGGT GTCATGTATT GATGTGGATG AATGTTTAGA AAATGCCACC 
ATATGCCCTC TGGGAAGCTG TGTGAACACA CTGGGGTCCT ATTTCTGTGC AGAGATTCCA 
GCGGGAATGC AGACCACAGG CCTCCCTTGG GAAACACAGG CAGCACATGG GAACTGGATG 
CCATTCCAGC CAACATCAGT AATGCCCCAG ACATCTGAGT CACTGACTAC TGGTGGCCCT 
CACCCTCTCA GGACCAGCAC TGAGTTAGCA CAAGGCATTT TCAGCACAAC TACTACCTCA 
GCACCACAAA GCACTTTGGC AAACACAAAC ATCATACCAC GTCCACAGCA CCTCAGAATT 
GGCCACCCTC CACTAGCTCA GAAAGCCTCT GGAATTTCCC ATATGCCTCT GAAAGAAAAC 
ATGATAGCCC AATCAGGTAC CACCACTGTA TCATTCACTA ACGTGCCAGA TTCCCACTGC 
TGGCATAACA AGGAGCTGCA TTTAAGTGGT AGTACATGGG CAGCACTGGG ATGTACTGAC 
TGCACTTGCC AGGATGGAAA TATATCTTGT GAAAAAAGGA TATGCACCCC AAACTGTTCA 
CACCCCGTCA TGTATCCTGA CAGCTGCTGC CCTATGTGTG ACGGATGCCT TTTTGAGGGA 
GTTTCCAGGG TAGATGGAGA GCTTTTCCCA AATTCTCCAG ACAATTGCAC AATCTGCATC 
TGCCTGGCTG GAAATGTCAC ATGTATCCCT CCTGTGTGCC CTCCTGTTAC ATGCACTGAC 
CCCTTCCTAT CAGACTGCTG CCTCAGATGC CCAGATGGAT GTGAATTCCA AGATCATTTT 
TATCCCCATG GATCCAAATT TAGCAGGGAT GATAATGGAT GCACCTCTTG TTTATGTCAG 
AACGGCGCTG TTGAATGTTC GTTTATTCCA TGTCCCAGTC TAGAATGTCC CAGAGAGAAC 
TGGGTGCTGG AGGCCGGCCA GTGTTGCTTT AAATGCCAGG AACCCATGCA AAGGACAGGG 
TGTCCTTTTG ATGATAATGG AATTGAAATT CCTGTTGGGC AGATCTGGTC GCCGGGAGAT 
CCCTGTGCAA TCTGCATTTG TCAGGCAGAC AGCTCCATCG TGTGCAAGAA AACAGACTGC 
GTGGAGACCT GTCCACATCC AATCACAGTG CCAGGGCAAT GCTGCCCAGA TTGCTCAGCG 
GGTTGTTCTT ATGGTAGAAG AACATTCAGG AACAATGAAA GCTTCCCCTC TAATTCAGAC 
CCATGTCTCA CTTGCATTTG TCTGATGGGC ACTGTAGCCT GTTCTCCAAT TGAGTGTGCA 
CTAAACTGCA CGTACCCTTT CCACGATGAA GGAGAGTGCT GCCCTGTTTG TCGAGATTGC 
ACATATGATG GCAGAAAAGT TCTGAATGGA CAGACTTTCT ACCTCGAGAG TGATCCTTGC 
ACTCAGTGCA CTTGTCAGCT TGGAGAGGTG CATTGTGAGG CAATCGTGTG CTCTGCTTCC 
TGCTCACATC CTTACGTCTT TCCTGGGGAA TGTTGCTCCT CTTGTGAAGA GTGTCTCTTT 
GAAGGCCATC TTTTAGAGAA TGGAGGCTCC TACATATTAA AGGTGGATCC CTGCATTGTG 
TGTCATTGTT CTGGTGGAAA TGTTCAATGT GAGCAAAGGG ATAATTCCTG TACTCCATGT 
GTGCAGAAGA CACAGGATTG TCTAAATGAA GTGCCAGATA TTTTTCATTA TAATCAGCTG 
CAAATCATGC CTGCGGATCT ACATCAGTCT GCTGGATCCC ACAGTCCAAA TATCGCACTG 
AAGCTACTGA ATGATGCAGT GATTTCCCAA CCCACCTATA TTCCTTTTTT CCAGAGATTA 
GTATCCAGGC GAAATACTGA GATCTTAGGA ACAATTTCAG CTGGTCCAAC TACTGTCCTT 
CATGGAAAAA CACCTGCAGA CTCAACTCCT CCTGGCTATA ATAAGGATTA TTTAACTACA 
AACCCTGTTG ACCCTTTGCC AAATGCACAC ACAACAATCA CCCACACAGG AGAAATAAAG 
TCTGTTTCAG CATCAGAGCC ATTCAGAAAC ATTATTATGC CTTCTTCAAA ATTGCCTCAA 
AGAGAAGCTA CATCATCAAA CTCATCTCCA CAACCTGAAG AATATTTGCA TGAATCTTTT 
CTGGGTTTGC ATACACAAAA GTTATCTACA CCTACCTCCT CTTTAGGCAT TCCTAATAAG 
TCTTTCCCTT CAGCAGTTAT TTCTACCTCA ACACTAATGC CTCAAATGTT TCCTTTTTCT 
GATATGTCTT CTGGTCCATC CAAACTAACT TCTGACAGCG CTGCCCTTCA TCCACCTGCT 
TGGAATGCAG ATAGTTCTGT TCAGTCACCA GCACATGATT TTATTTCTTC TTTCACCCAT 
ACACCGGACT CACATTCTTT ATTTACCCAC TCACAAAGAC CCCTCTCTTC TGGCACTTCT 
GATATATCAT CTATCCATGC AGATACCACT CAATTCTCTT TGTTAGCCAA AAACACTCTA 
GGTTATACCT CGGCCCCTGC ACCCCATGCT GCTCACTTCC CAACCTCTGC AAACATCATC 
CCGAGTGATT CTGGGATTCC TCTCATGCAG CTAAACCACA GAGAGTCAGA GAGTGATGAA 
CTGGGGAATG GACACGCAAT TGGATGTTCT TTAAAAGGTC TTGAATTTTC CAGTGGCTCC 
ATCTTTGTTG CTGACATGGA CTACTGCCTG CCCTGTGAAT GTTTGAATGG AGAGATCTAC 
TGTTCACACC AAGATTTATT CCCCAGTGGA CTGTGTTGCC AGAGCTGTAG CCACCAGGGT 
GTTGATTCTT GTGCAGGGAA AATAAACCAG GTCCATTCAG AAAGTATGAG GAGTCAGAAC 
CTTTGCAATT GCAGAAATCT CAGTCTTCAC TGTGACTCTT GCTCGTTCAG AGAAGAATGC 
AGAAGAAATG GCTCTACACA AACCGCCAAG GAAGAACAAC TACCTGAGAA CATCCACTGG 
GCTCCACTTA TATTTGAAGG GGATGTTGAG CCTCAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwce ( XM_018093325.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948814.2 Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X1, mRNA.

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CloneID	OXa56582
Clone ID Related Accession (Same CDS sequence)	XM_018093325.2
Accession Version	XM_018093325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor C and EGF domain-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTGC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCAAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATTGAT GAATGTTCCT CTAAAAGATC CCTGTGCCAA 
CAAAAATGCA AGAACCTTCA GGGCAGCTAC AGGTGTTTGT GTGCGACGGG GTTCGTCCTT 
CAGAGCAATG GACACACCTG TGCAGACATA AATGAATGTC GGAGGCCAGG AACATCCCAT 
CTTTGCAAAC ACTTCTGCCA CAATACTCAT GGAAGTTTCT TCTGTTCTTG CCGGGCTGGG 
TTTCTGCTTG ATCCAGATAA GGTGTCATGT ATTGATGTGG ATGAATGTTT AGAAAATGCC 
ACCATATGCC CTCTGGGAAG CTGTGTGAAC ACACTGGGGT CCTATTTCTG TGCAGAGATT 
CCAGCGGGAA TGCAGACCAC AGGCCTCCCT TGGGAAACAC AGGCAGCACA TGGGAACTGG 
ATGCCATTCC AGCCAACATC AGTAATGCCC CAGACATCTG AGTCACTGAC TACTGGTGGC 
CCTCACCCTC TCAGGACCAG CACTGAGTTA GCACAAGGCA TTTTCAGCAC AACTACTACC 
TCAGCGCCAC AAAGCACTTT GGCAAACACA AACATCATAC CACGTCCACA GCACCTCAGA 
ATTGGCCACC CTCCACTAGC TCAGAAAGCC TCTGGAATTT CCCATATGCC TCTGAAAGAA 
AACATGATAG CCCAATCAGG TACCACCACT GTATCATTCA CTAACGTGCC AGATTCCCAC 
TGCTGGCATA ACAAGGAGCT GCATTTAAGT GGTAGTACAT GGGCAGCACT GGGATGTACT 
GACTGCACTT GCCAGGATGG AAATATATCT TGTGAAAAAA GGATATGCAC CCCAAACTGT 
TCACACCCCG TCATGTATCC TGACAGCTGC TGCCCTATGT GTGACGGATG CCTTTTTGAG 
GGAGTTTCCA GGGTAGATGG AGAGCTTTTC CCAAATTCTC CAGACAATTG CACAATCTGC 
ATCTGCCTGG CTGGAAATGT CACATGTATC CCTCCTGTGT GCCCTCCTGT TACATGCACT 
GACCCCTTCC TATCAGACTG CTGCCTCAGA TGCCCAGATG GATGTGAATT CCAAGATCAT 
TTTTATCCCC ATGGATCCAA ATTTAGCAGG GATGATAATG GATGCACCTC TTGTTTATGT 
CAGAACGGCG CTGTTGAATG TTCGTTTATT CCATGTCCCA GTCTAGAATG TCCCAGAGAG 
AACTGGGTGC TGGAGGCCGG CCAGTGTTGC TTTAAATGCC AGGAACCCAT GCAAAGGACA 
GGGTGTCCTT TTGATGATAA TGGAATTGAA ATTCCTGTTG GGCAGATCTG GTCGCCGGGA 
GATCCCTGTG CAATCTGCAT TTGTCAGGCA GACAGCTCCA TCGTGTGCAA GAAAACAGAC 
TGCGTGGAGA CCTGTCCACA TCCAATCACA GTGCCAGGGC AATGCTGCCC AGATTGCTCA 
GCGGGTTGTT CTTATGGTAG AAGAACATTC AGGAACAATG AAAGCTTCCC CTCTAATTCA 
GACCCATGTC TCACTTGCAT TTGTCTGATG GGCACTGTAG CCTGTTCTCC AATTGAGTGT 
GCAGTAAACT GCACGTACCC TTTCCACGAT GAAGGAGAGT GCTGCCCTGT TTGTCGAGAT 
TGCACATATG ATGGCAGAAA AGTTCTGAAT GAACAGACTT TCTACCTCGA GAGTGATCCT 
TGCACTCAGT GCACTTGTCA GCTTGGAGAG GTGCATTGTG AGGCAATCGT GTGCTCTGCT 
TCCTGCTCAC ATCCTTATGT CTTTCCTGGG GAATGTTGCT CCTCTTGTGA AGAGTGTCTC 
TTTGAAGGCC ATCTTTTAGA GAATGGAGGC TCCTACATAT TAAAGGTGGA TCCCTGCATT 
GTGTGTCATT GTTCTGGTGG AAATGTTCAA TGTGAGCAAA GGGATAATTC CTGTACTCCA 
TGTGTGCAGA AGACACAGGA TTGTCTAAAT GAAGTGCCAG ATATTTTTCA TTATAATCAG 
CTGCAAATCA TGCCTGCGGA TCTACATCAG TCTGCTGGAT CCCACAGTCC AAATATCGCA 
CTGAAGCTAC TGAATGATGC AGTGATTTCC CAACCCACCT ATATTCCTTT TTTCCAAAGA 
TTAGTATCCA GGCGAAATAC TGAGATCTTA GGAACAATTT CAGCTGGTCC AACTACTGTC 
CTTCATGGAA AAACACCTGC AGACTCAACT CCTCCTGGCT ATAATAAGGA TTATTTAACT 
ACAAACCCTG TTGACCCTTT GCCAAATGCA CACACAACAA TCACCCACAC AGGAGAAATA 
AAGTCTGTTT CAGCATCAGA GCCATTCAGA AACATTATTA TGCCTTCTTC AAAATTGCCT 
CAAAGAGAAG CTACATCATC AAACTCATCT CCACAACCTG AAGAATATTT GCATGAATCT 
TTTCTGGGTT TGCATACACA AAAGTTATCT ACACCTACCT CCTCTTTAGG CATTCCTAAT 
AAGTCTTTCC CTTCAGCAGT TATTTCTACC TCAACACTAA TGCCTCAAAT GTTTCCTTTT 
TCTGATATGT CTTCTGGTCC ATCCAAACTA ACTTCTGACA GCGCTGCCCT TCATCCACCT 
GCTTGGAATG CAGATAGTTC TGTTCAGTCA CCAGCACATG ATTTTATTTC TTCTTTCACC 
CATACACCGG ACTCACATTC TTTATTTACC CACTCACAAA GACCCCTCTC TTCTGGCACT 
TCTGATATAT CATCTATCCA TGCAGATACC ACTCAATTCT CTTTGTTAGC CAAAAACACT 
CTAGGCTATA CCTCGGCCCC TGCACCCCAT GCTGCTCACT TCCCAACCTC TGCAAACATC 
ATCCCGAGTG ATTCTGGGAT TCCTCTCATG CAGCTAAACC ACAGAGAGTC AGAGAGTGAT 
GAACTGGGGA ATGGACACGC AATTGGATGT TCTTTAAAAG GTCTTGAATT TTCCAGTGGC 
TCCATCTTTG TTGCTGACAT GGACTACTGC CTGCCCTGTG AATGTTTGAA TGGAGAGATC 
TACTGTTCAC ACCAAGATTT ATTCCCCAGT GGACTGTGTT GCCAGAGCTG TAGCCACCAG 
GGTGTTGATT CTTGTGCAGG GAAAATAAAC CAGGTCCATT CAGAAAGTAT GAGGAGTCAG 
AACCTTTGCA ATTGCAGAAA TCTCAGTCTT CACTGTGACT CTTGCTCGTT CAGAGAAGAA 
TGCAGAAGAA ATGGCTCTAC ACAAACCGCC AAGGAAGAAC AACTACCTGA GAACATCCAC 
TGGGCTCCAC TTATATTTGA AGGGGATGTT GAGCCTCAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948814.2
CDS	618..4742
Translation	MLPFWLGMSCLLLLISETGGRVYKGRKKPPALTAQRLRSGPHVCFSGMGAGCCPGWTVSPGTGQCLQPLCSFGCASGFCIAPNVCSCRDGHQGVTCADEFSSEHEKTLEDKGTPLTCLSALCDQNCRLVNGVPSCSCFHGYSLGKDGRTCYDVDECSRPQASSLCQQQCKNSIGSYRCLCFYGYQISPNGRSCIPTKNLNFIAGIAAPSPCGEYGCELMCNDGGCEHVSRVCPVGFRMAETVNGVTCTDIDECEASSCEGTCINTEGGFVCDCGPGMKLSTDRTSCYDIDECSSKRSLCQQKCKNLQGSYRCLCATGFVLQSNGHTCADINECRRPGTSHLCKHFCHNTHGSFFCSCRAGFLLDPDKVSCIDVDECLENATICPLGSCVNTLGSYFCAEIPAGMQTTGLPWETQAAHGNWMPFQPTSVMPQTSESLTTGGPHPLRTSTELAQGIFSTTTTSAPQSTLANTNIIPRPQHLRIGHPPLAQKASGISHMPLKENMIAQSGTTTVSFTNVPDSHCWHNKELHLSGSTWAALGCTDCTCQDGNISCEKRICTPNCSHPVMYPDSCCPMCDGCLFEGVSRVDGELFPNSPDNCTICICLAGNVTCIPPVCPPVTCTDPFLSDCCLRCPDGCEFQDHFYPHGSKFSRDDNGCTSCLCQNGAVECSFIPCPSLECPRENWVLEAGQCCFKCQEPMQRTGCPFDDNGIEIPVGQIWSPGDPCAICICQADSSIVCKKTDCVETCPHPITVPGQCCPDCSAGCSYGRRTFRNNESFPSNSDPCLTCICLMGTVACSPIECAVNCTYPFHDEGECCPVCRDCTYDGRKVLNEQTFYLESDPCTQCTCQLGEVHCEAIVCSASCSHPYVFPGECCSSCEECLFEGHLLENGGSYILKVDPCIVCHCSGGNVQCEQRDNSCTPCVQKTQDCLNEVPDIFHYNQLQIMPADLHQSAGSHSPNIALKLLNDAVISQPTYIPFFQRLVSRRNTEILGTISAGPTTVLHGKTPADSTPPGYNKDYLTTNPVDPLPNAHTTITHTGEIKSVSASEPFRNIIMPSSKLPQREATSSNSSPQPEEYLHESFLGLHTQKLSTPTSSLGIPNKSFPSAVISTSTLMPQMFPFSDMSSGPSKLTSDSAALHPPAWNADSSVQSPAHDFISSFTHTPDSHSLFTHSQRPLSSGTSDISSIHADTTQFSLLAKNTLGYTSAPAPHAAHFPTSANIIPSDSGIPLMQLNHRESESDELGNGHAIGCSLKGLEFSSGSIFVADMDYCLPCECLNGEIYCSHQDLFPSGLCCQSCSHQGVDSCAGKINQVHSESMRSQNLCNCRNLSLHCDSCSFREECRRNGSTQTAKEEQLPENIHWAPLIFEGDVEPQR

Target ORF information:

RefSeq Version	XM_018093325.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093325.2

ORF Insert Sequence:

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTGC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCAAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATTGAT GAATGTTCCT CTAAAAGATC CCTGTGCCAA 
CAAAAATGCA AGAACCTTCA GGGCAGCTAC AGGTGTTTGT GTGCGACGGG GTTCGTCCTT 
CAGAGCAATG GACACACCTG TGCAGACATA AATGAATGTC GGAGGCCAGG AACATCCCAT 
CTTTGCAAAC ACTTCTGCCA CAATACTCAT GGAAGTTTCT TCTGTTCTTG CCGGGCTGGG 
TTTCTGCTTG ATCCAGATAA GGTGTCATGT ATTGATGTGG ATGAATGTTT AGAAAATGCC 
ACCATATGCC CTCTGGGAAG CTGTGTGAAC ACACTGGGGT CCTATTTCTG TGCAGAGATT 
CCAGCGGGAA TGCAGACCAC AGGCCTCCCT TGGGAAACAC AGGCAGCACA TGGGAACTGG 
ATGCCATTCC AGCCAACATC AGTAATGCCC CAGACATCTG AGTCACTGAC TACTGGTGGC 
CCTCACCCTC TCAGGACCAG CACTGAGTTA GCACAAGGCA TTTTCAGCAC AACTACTACC 
TCAGCGCCAC AAAGCACTTT GGCAAACACA AACATCATAC CACGTCCACA GCACCTCAGA 
ATTGGCCACC CTCCACTAGC TCAGAAAGCC TCTGGAATTT CCCATATGCC TCTGAAAGAA 
AACATGATAG CCCAATCAGG TACCACCACT GTATCATTCA CTAACGTGCC AGATTCCCAC 
TGCTGGCATA ACAAGGAGCT GCATTTAAGT GGTAGTACAT GGGCAGCACT GGGATGTACT 
GACTGCACTT GCCAGGATGG AAATATATCT TGTGAAAAAA GGATATGCAC CCCAAACTGT 
TCACACCCCG TCATGTATCC TGACAGCTGC TGCCCTATGT GTGACGGATG CCTTTTTGAG 
GGAGTTTCCA GGGTAGATGG AGAGCTTTTC CCAAATTCTC CAGACAATTG CACAATCTGC 
ATCTGCCTGG CTGGAAATGT CACATGTATC CCTCCTGTGT GCCCTCCTGT TACATGCACT 
GACCCCTTCC TATCAGACTG CTGCCTCAGA TGCCCAGATG GATGTGAATT CCAAGATCAT 
TTTTATCCCC ATGGATCCAA ATTTAGCAGG GATGATAATG GATGCACCTC TTGTTTATGT 
CAGAACGGCG CTGTTGAATG TTCGTTTATT CCATGTCCCA GTCTAGAATG TCCCAGAGAG 
AACTGGGTGC TGGAGGCCGG CCAGTGTTGC TTTAAATGCC AGGAACCCAT GCAAAGGACA 
GGGTGTCCTT TTGATGATAA TGGAATTGAA ATTCCTGTTG GGCAGATCTG GTCGCCGGGA 
GATCCCTGTG CAATCTGCAT TTGTCAGGCA GACAGCTCCA TCGTGTGCAA GAAAACAGAC 
TGCGTGGAGA CCTGTCCACA TCCAATCACA GTGCCAGGGC AATGCTGCCC AGATTGCTCA 
GCGGGTTGTT CTTATGGTAG AAGAACATTC AGGAACAATG AAAGCTTCCC CTCTAATTCA 
GACCCATGTC TCACTTGCAT TTGTCTGATG GGCACTGTAG CCTGTTCTCC AATTGAGTGT 
GCAGTAAACT GCACGTACCC TTTCCACGAT GAAGGAGAGT GCTGCCCTGT TTGTCGAGAT 
TGCACATATG ATGGCAGAAA AGTTCTGAAT GAACAGACTT TCTACCTCGA GAGTGATCCT 
TGCACTCAGT GCACTTGTCA GCTTGGAGAG GTGCATTGTG AGGCAATCGT GTGCTCTGCT 
TCCTGCTCAC ATCCTTATGT CTTTCCTGGG GAATGTTGCT CCTCTTGTGA AGAGTGTCTC 
TTTGAAGGCC ATCTTTTAGA GAATGGAGGC TCCTACATAT TAAAGGTGGA TCCCTGCATT 
GTGTGTCATT GTTCTGGTGG AAATGTTCAA TGTGAGCAAA GGGATAATTC CTGTACTCCA 
TGTGTGCAGA AGACACAGGA TTGTCTAAAT GAAGTGCCAG ATATTTTTCA TTATAATCAG 
CTGCAAATCA TGCCTGCGGA TCTACATCAG TCTGCTGGAT CCCACAGTCC AAATATCGCA 
CTGAAGCTAC TGAATGATGC AGTGATTTCC CAACCCACCT ATATTCCTTT TTTCCAAAGA 
TTAGTATCCA GGCGAAATAC TGAGATCTTA GGAACAATTT CAGCTGGTCC AACTACTGTC 
CTTCATGGAA AAACACCTGC AGACTCAACT CCTCCTGGCT ATAATAAGGA TTATTTAACT 
ACAAACCCTG TTGACCCTTT GCCAAATGCA CACACAACAA TCACCCACAC AGGAGAAATA 
AAGTCTGTTT CAGCATCAGA GCCATTCAGA AACATTATTA TGCCTTCTTC AAAATTGCCT 
CAAAGAGAAG CTACATCATC AAACTCATCT CCACAACCTG AAGAATATTT GCATGAATCT 
TTTCTGGGTT TGCATACACA AAAGTTATCT ACACCTACCT CCTCTTTAGG CATTCCTAAT 
AAGTCTTTCC CTTCAGCAGT TATTTCTACC TCAACACTAA TGCCTCAAAT GTTTCCTTTT 
TCTGATATGT CTTCTGGTCC ATCCAAACTA ACTTCTGACA GCGCTGCCCT TCATCCACCT 
GCTTGGAATG CAGATAGTTC TGTTCAGTCA CCAGCACATG ATTTTATTTC TTCTTTCACC 
CATACACCGG ACTCACATTC TTTATTTACC CACTCACAAA GACCCCTCTC TTCTGGCACT 
TCTGATATAT CATCTATCCA TGCAGATACC ACTCAATTCT CTTTGTTAGC CAAAAACACT 
CTAGGCTATA CCTCGGCCCC TGCACCCCAT GCTGCTCACT TCCCAACCTC TGCAAACATC 
ATCCCGAGTG ATTCTGGGAT TCCTCTCATG CAGCTAAACC ACAGAGAGTC AGAGAGTGAT 
GAACTGGGGA ATGGACACGC AATTGGATGT TCTTTAAAAG GTCTTGAATT TTCCAGTGGC 
TCCATCTTTG TTGCTGACAT GGACTACTGC CTGCCCTGTG AATGTTTGAA TGGAGAGATC 
TACTGTTCAC ACCAAGATTT ATTCCCCAGT GGACTGTGTT GCCAGAGCTG TAGCCACCAG 
GGTGTTGATT CTTGTGCAGG GAAAATAAAC CAGGTCCATT CAGAAAGTAT GAGGAGTCAG 
AACCTTTGCA ATTGCAGAAA TCTCAGTCTT CACTGTGACT CTTGCTCGTT CAGAGAAGAA 
TGCAGAAGAA ATGGCTCTAC ACAAACCGCC AAGGAAGAAC AACTACCTGA GAACATCCAC 
TGGGCTCCAC TTATATTTGA AGGGGATGTT GAGCCTCAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwce ( XM_018093326.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948815.2 Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa56583
Clone ID Related Accession (Same CDS sequence)	XM_018093326.2
Accession Version	XM_018093326.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor C and EGF domain-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTGC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCAAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATTGAT GAATGTTCCT CTAAAAGATC CCTGTGCCAA 
CAAAAATGCA AGAACCTTCA GGGCAGCTAC AGGTGTTTGT GTGCGACGGG GTTCGTCCTT 
CAGAGCAATG GACACACCTG TGCAGACATA AATGAATGTC GGAGGCCAGG AACATCCCAT 
CTTTGCAAAC ACTTCTGCCA CAATACTCAT GGAAGTTTCT TCTGTTCTTG CCGGGCTGGG 
TTTCTGCTTG ATCCAGATAA GGTGTCATGT ATTGAGATTC CAGCGGGAAT GCAGACCACA 
GGCCTCCCTT GGGAAACACA GGCAGCACAT GGGAACTGGA TGCCATTCCA GCCAACATCA 
GTAATGCCCC AGACATCTGA GTCACTGACT ACTGGTGGCC CTCACCCTCT CAGGACCAGC 
ACTGAGTTAG CACAAGGCAT TTTCAGCACA ACTACTACCT CAGCGCCACA AAGCACTTTG 
GCAAACACAA ACATCATACC ACGTCCACAG CACCTCAGAA TTGGCCACCC TCCACTAGCT 
CAGAAAGCCT CTGGAATTTC CCATATGCCT CTGAAAGAAA ACATGATAGC CCAATCAGGT 
ACCACCACTG TATCATTCAC TAACGTGCCA GATTCCCACT GCTGGCATAA CAAGGAGCTG 
CATTTAAGTG GTAGTACATG GGCAGCACTG GGATGTACTG ACTGCACTTG CCAGGATGGA 
AATATATCTT GTGAAAAAAG GATATGCACC CCAAACTGTT CACACCCCGT CATGTATCCT 
GACAGCTGCT GCCCTATGTG TGACGGATGC CTTTTTGAGG GAGTTTCCAG GGTAGATGGA 
GAGCTTTTCC CAAATTCTCC AGACAATTGC ACAATCTGCA TCTGCCTGGC TGGAAATGTC 
ACATGTATCC CTCCTGTGTG CCCTCCTGTT ACATGCACTG ACCCCTTCCT ATCAGACTGC 
TGCCTCAGAT GCCCAGATGG ATGTGAATTC CAAGATCATT TTTATCCCCA TGGATCCAAA 
TTTAGCAGGG ATGATAATGG ATGCACCTCT TGTTTATGTC AGAACGGCGC TGTTGAATGT 
TCGTTTATTC CATGTCCCAG TCTAGAATGT CCCAGAGAGA ACTGGGTGCT GGAGGCCGGC 
CAGTGTTGCT TTAAATGCCA GGAACCCATG CAAAGGACAG GGTGTCCTTT TGATGATAAT 
GGAATTGAAA TTCCTGTTGG GCAGATCTGG TCGCCGGGAG ATCCCTGTGC AATCTGCATT 
TGTCAGGCAG ACAGCTCCAT CGTGTGCAAG AAAACAGACT GCGTGGAGAC CTGTCCACAT 
CCAATCACAG TGCCAGGGCA ATGCTGCCCA GATTGCTCAG CGGGTTGTTC TTATGGTAGA 
AGAACATTCA GGAACAATGA AAGCTTCCCC TCTAATTCAG ACCCATGTCT CACTTGCATT 
TGTCTGATGG GCACTGTAGC CTGTTCTCCA ATTGAGTGTG CAGTAAACTG CACGTACCCT 
TTCCACGATG AAGGAGAGTG CTGCCCTGTT TGTCGAGATT GCACATATGA TGGCAGAAAA 
GTTCTGAATG AACAGACTTT CTACCTCGAG AGTGATCCTT GCACTCAGTG CACTTGTCAG 
CTTGGAGAGG TGCATTGTGA GGCAATCGTG TGCTCTGCTT CCTGCTCACA TCCTTATGTC 
TTTCCTGGGG AATGTTGCTC CTCTTGTGAA GAGTGTCTCT TTGAAGGCCA TCTTTTAGAG 
AATGGAGGCT CCTACATATT AAAGGTGGAT CCCTGCATTG TGTGTCATTG TTCTGGTGGA 
AATGTTCAAT GTGAGCAAAG GGATAATTCC TGTACTCCAT GTGTGCAGAA GACACAGGAT 
TGTCTAAATG AAGTGCCAGA TATTTTTCAT TATAATCAGC TGCAAATCAT GCCTGCGGAT 
CTACATCAGT CTGCTGGATC CCACAGTCCA AATATCGCAC TGAAGCTACT GAATGATGCA 
GTGATTTCCC AACCCACCTA TATTCCTTTT TTCCAAAGAT TAGTATCCAG GCGAAATACT 
GAGATCTTAG GAACAATTTC AGCTGGTCCA ACTACTGTCC TTCATGGAAA AACACCTGCA 
GACTCAACTC CTCCTGGCTA TAATAAGGAT TATTTAACTA CAAACCCTGT TGACCCTTTG 
CCAAATGCAC ACACAACAAT CACCCACACA GGAGAAATAA AGTCTGTTTC AGCATCAGAG 
CCATTCAGAA ACATTATTAT GCCTTCTTCA AAATTGCCTC AAAGAGAAGC TACATCATCA 
AACTCATCTC CACAACCTGA AGAATATTTG CATGAATCTT TTCTGGGTTT GCATACACAA 
AAGTTATCTA CACCTACCTC CTCTTTAGGC ATTCCTAATA AGTCTTTCCC TTCAGCAGTT 
ATTTCTACCT CAACACTAAT GCCTCAAATG TTTCCTTTTT CTGATATGTC TTCTGGTCCA 
TCCAAACTAA CTTCTGACAG CGCTGCCCTT CATCCACCTG CTTGGAATGC AGATAGTTCT 
GTTCAGTCAC CAGCACATGA TTTTATTTCT TCTTTCACCC ATACACCGGA CTCACATTCT 
TTATTTACCC ACTCACAAAG ACCCCTCTCT TCTGGCACTT CTGATATATC ATCTATCCAT 
GCAGATACCA CTCAATTCTC TTTGTTAGCC AAAAACACTC TAGGCTATAC CTCGGCCCCT 
GCACCCCATG CTGCTCACTT CCCAACCTCT GCAAACATCA TCCCGAGTGA TTCTGGGATT 
CCTCTCATGC AGCTAAACCA CAGAGAGTCA GAGAGTGATG AACTGGGGAA TGGACACGCA 
ATTGGATGTT CTTTAAAAGG TCTTGAATTT TCCAGTGGCT CCATCTTTGT TGCTGACATG 
GACTACTGCC TGCCCTGTGA ATGTTTGAAT GGAGAGATCT ACTGTTCACA CCAAGATTTA 
TTCCCCAGTG GACTGTGTTG CCAGAGCTGT AGCCACCAGG GTGTTGATTC TTGTGCAGGG 
AAAATAAACC AGGTCCATTC AGAAAGTATG AGGAGTCAGA ACCTTTGCAA TTGCAGAAAT 
CTCAGTCTTC ACTGTGACTC TTGCTCGTTC AGAGAAGAAT GCAGAAGAAA TGGCTCTACA 
CAAACCGCCA AGGAAGAACA ACTACCTGAG AACATCCACT GGGCTCCACT TATATTTGAA 
GGGGATGTTG AGCCTCAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948815.2
CDS	619..4662
Translation	MLPFWLGMSCLLLLISETGGRVYKGRKKPPALTAQRLRSGPHVCFSGMGAGCCPGWTVSPGTGQCLQPLCSFGCASGFCIAPNVCSCRDGHQGVTCADEFSSEHEKTLEDKGTPLTCLSALCDQNCRLVNGVPSCSCFHGYSLGKDGRTCYDVDECSRPQASSLCQQQCKNSIGSYRCLCFYGYQISPNGRSCIPTKNLNFIAGIAAPSPCGEYGCELMCNDGGCEHVSRVCPVGFRMAETVNGVTCTDIDECEASSCEGTCINTEGGFVCDCGPGMKLSTDRTSCYDIDECSSKRSLCQQKCKNLQGSYRCLCATGFVLQSNGHTCADINECRRPGTSHLCKHFCHNTHGSFFCSCRAGFLLDPDKVSCIEIPAGMQTTGLPWETQAAHGNWMPFQPTSVMPQTSESLTTGGPHPLRTSTELAQGIFSTTTTSAPQSTLANTNIIPRPQHLRIGHPPLAQKASGISHMPLKENMIAQSGTTTVSFTNVPDSHCWHNKELHLSGSTWAALGCTDCTCQDGNISCEKRICTPNCSHPVMYPDSCCPMCDGCLFEGVSRVDGELFPNSPDNCTICICLAGNVTCIPPVCPPVTCTDPFLSDCCLRCPDGCEFQDHFYPHGSKFSRDDNGCTSCLCQNGAVECSFIPCPSLECPRENWVLEAGQCCFKCQEPMQRTGCPFDDNGIEIPVGQIWSPGDPCAICICQADSSIVCKKTDCVETCPHPITVPGQCCPDCSAGCSYGRRTFRNNESFPSNSDPCLTCICLMGTVACSPIECAVNCTYPFHDEGECCPVCRDCTYDGRKVLNEQTFYLESDPCTQCTCQLGEVHCEAIVCSASCSHPYVFPGECCSSCEECLFEGHLLENGGSYILKVDPCIVCHCSGGNVQCEQRDNSCTPCVQKTQDCLNEVPDIFHYNQLQIMPADLHQSAGSHSPNIALKLLNDAVISQPTYIPFFQRLVSRRNTEILGTISAGPTTVLHGKTPADSTPPGYNKDYLTTNPVDPLPNAHTTITHTGEIKSVSASEPFRNIIMPSSKLPQREATSSNSSPQPEEYLHESFLGLHTQKLSTPTSSLGIPNKSFPSAVISTSTLMPQMFPFSDMSSGPSKLTSDSAALHPPAWNADSSVQSPAHDFISSFTHTPDSHSLFTHSQRPLSSGTSDISSIHADTTQFSLLAKNTLGYTSAPAPHAAHFPTSANIIPSDSGIPLMQLNHRESESDELGNGHAIGCSLKGLEFSSGSIFVADMDYCLPCECLNGEIYCSHQDLFPSGLCCQSCSHQGVDSCAGKINQVHSESMRSQNLCNCRNLSLHCDSCSFREECRRNGSTQTAKEEQLPENIHWAPLIFEGDVEPQR

Target ORF information:

RefSeq Version	XM_018093326.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093326.2

ORF Insert Sequence:

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTGC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCAAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATTGAT GAATGTTCCT CTAAAAGATC CCTGTGCCAA 
CAAAAATGCA AGAACCTTCA GGGCAGCTAC AGGTGTTTGT GTGCGACGGG GTTCGTCCTT 
CAGAGCAATG GACACACCTG TGCAGACATA AATGAATGTC GGAGGCCAGG AACATCCCAT 
CTTTGCAAAC ACTTCTGCCA CAATACTCAT GGAAGTTTCT TCTGTTCTTG CCGGGCTGGG 
TTTCTGCTTG ATCCAGATAA GGTGTCATGT ATTGAGATTC CAGCGGGAAT GCAGACCACA 
GGCCTCCCTT GGGAAACACA GGCAGCACAT GGGAACTGGA TGCCATTCCA GCCAACATCA 
GTAATGCCCC AGACATCTGA GTCACTGACT ACTGGTGGCC CTCACCCTCT CAGGACCAGC 
ACTGAGTTAG CACAAGGCAT TTTCAGCACA ACTACTACCT CAGCGCCACA AAGCACTTTG 
GCAAACACAA ACATCATACC ACGTCCACAG CACCTCAGAA TTGGCCACCC TCCACTAGCT 
CAGAAAGCCT CTGGAATTTC CCATATGCCT CTGAAAGAAA ACATGATAGC CCAATCAGGT 
ACCACCACTG TATCATTCAC TAACGTGCCA GATTCCCACT GCTGGCATAA CAAGGAGCTG 
CATTTAAGTG GTAGTACATG GGCAGCACTG GGATGTACTG ACTGCACTTG CCAGGATGGA 
AATATATCTT GTGAAAAAAG GATATGCACC CCAAACTGTT CACACCCCGT CATGTATCCT 
GACAGCTGCT GCCCTATGTG TGACGGATGC CTTTTTGAGG GAGTTTCCAG GGTAGATGGA 
GAGCTTTTCC CAAATTCTCC AGACAATTGC ACAATCTGCA TCTGCCTGGC TGGAAATGTC 
ACATGTATCC CTCCTGTGTG CCCTCCTGTT ACATGCACTG ACCCCTTCCT ATCAGACTGC 
TGCCTCAGAT GCCCAGATGG ATGTGAATTC CAAGATCATT TTTATCCCCA TGGATCCAAA 
TTTAGCAGGG ATGATAATGG ATGCACCTCT TGTTTATGTC AGAACGGCGC TGTTGAATGT 
TCGTTTATTC CATGTCCCAG TCTAGAATGT CCCAGAGAGA ACTGGGTGCT GGAGGCCGGC 
CAGTGTTGCT TTAAATGCCA GGAACCCATG CAAAGGACAG GGTGTCCTTT TGATGATAAT 
GGAATTGAAA TTCCTGTTGG GCAGATCTGG TCGCCGGGAG ATCCCTGTGC AATCTGCATT 
TGTCAGGCAG ACAGCTCCAT CGTGTGCAAG AAAACAGACT GCGTGGAGAC CTGTCCACAT 
CCAATCACAG TGCCAGGGCA ATGCTGCCCA GATTGCTCAG CGGGTTGTTC TTATGGTAGA 
AGAACATTCA GGAACAATGA AAGCTTCCCC TCTAATTCAG ACCCATGTCT CACTTGCATT 
TGTCTGATGG GCACTGTAGC CTGTTCTCCA ATTGAGTGTG CAGTAAACTG CACGTACCCT 
TTCCACGATG AAGGAGAGTG CTGCCCTGTT TGTCGAGATT GCACATATGA TGGCAGAAAA 
GTTCTGAATG AACAGACTTT CTACCTCGAG AGTGATCCTT GCACTCAGTG CACTTGTCAG 
CTTGGAGAGG TGCATTGTGA GGCAATCGTG TGCTCTGCTT CCTGCTCACA TCCTTATGTC 
TTTCCTGGGG AATGTTGCTC CTCTTGTGAA GAGTGTCTCT TTGAAGGCCA TCTTTTAGAG 
AATGGAGGCT CCTACATATT AAAGGTGGAT CCCTGCATTG TGTGTCATTG TTCTGGTGGA 
AATGTTCAAT GTGAGCAAAG GGATAATTCC TGTACTCCAT GTGTGCAGAA GACACAGGAT 
TGTCTAAATG AAGTGCCAGA TATTTTTCAT TATAATCAGC TGCAAATCAT GCCTGCGGAT 
CTACATCAGT CTGCTGGATC CCACAGTCCA AATATCGCAC TGAAGCTACT GAATGATGCA 
GTGATTTCCC AACCCACCTA TATTCCTTTT TTCCAAAGAT TAGTATCCAG GCGAAATACT 
GAGATCTTAG GAACAATTTC AGCTGGTCCA ACTACTGTCC TTCATGGAAA AACACCTGCA 
GACTCAACTC CTCCTGGCTA TAATAAGGAT TATTTAACTA CAAACCCTGT TGACCCTTTG 
CCAAATGCAC ACACAACAAT CACCCACACA GGAGAAATAA AGTCTGTTTC AGCATCAGAG 
CCATTCAGAA ACATTATTAT GCCTTCTTCA AAATTGCCTC AAAGAGAAGC TACATCATCA 
AACTCATCTC CACAACCTGA AGAATATTTG CATGAATCTT TTCTGGGTTT GCATACACAA 
AAGTTATCTA CACCTACCTC CTCTTTAGGC ATTCCTAATA AGTCTTTCCC TTCAGCAGTT 
ATTTCTACCT CAACACTAAT GCCTCAAATG TTTCCTTTTT CTGATATGTC TTCTGGTCCA 
TCCAAACTAA CTTCTGACAG CGCTGCCCTT CATCCACCTG CTTGGAATGC AGATAGTTCT 
GTTCAGTCAC CAGCACATGA TTTTATTTCT TCTTTCACCC ATACACCGGA CTCACATTCT 
TTATTTACCC ACTCACAAAG ACCCCTCTCT TCTGGCACTT CTGATATATC ATCTATCCAT 
GCAGATACCA CTCAATTCTC TTTGTTAGCC AAAAACACTC TAGGCTATAC CTCGGCCCCT 
GCACCCCATG CTGCTCACTT CCCAACCTCT GCAAACATCA TCCCGAGTGA TTCTGGGATT 
CCTCTCATGC AGCTAAACCA CAGAGAGTCA GAGAGTGATG AACTGGGGAA TGGACACGCA 
ATTGGATGTT CTTTAAAAGG TCTTGAATTT TCCAGTGGCT CCATCTTTGT TGCTGACATG 
GACTACTGCC TGCCCTGTGA ATGTTTGAAT GGAGAGATCT ACTGTTCACA CCAAGATTTA 
TTCCCCAGTG GACTGTGTTG CCAGAGCTGT AGCCACCAGG GTGTTGATTC TTGTGCAGGG 
AAAATAAACC AGGTCCATTC AGAAAGTATG AGGAGTCAGA ACCTTTGCAA TTGCAGAAAT 
CTCAGTCTTC ACTGTGACTC TTGCTCGTTC AGAGAAGAAT GCAGAAGAAA TGGCTCTACA 
CAAACCGCCA AGGAAGAACA ACTACCTGAG AACATCCACT GGGCTCCACT TATATTTGAA 
GGGGATGTTG AGCCTCAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwce ( XM_018093327.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948816.2 Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X3, mRNA.

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CloneID	OXa56584
Clone ID Related Accession (Same CDS sequence)	XM_018093327.2
Accession Version	XM_018093327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor C and EGF domain-containing protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTGC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCAAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATAAAT GAATGTCGGA GGCCAGGAAC ATCCCATCTT 
TGCAAACACT TCTGCCACAA TACTCATGGA AGTTTCTTCT GTTCTTGCCG GGCTGGGTTT 
CTGCTTGATC CAGATAAGGT GTCATGTATT GATGTGGATG AATGTTTAGA AAATGCCACC 
ATATGCCCTC TGGGAAGCTG TGTGAACACA CTGGGGTCCT ATTTCTGTGC AGAGATTCCA 
GCGGGAATGC AGACCACAGG CCTCCCTTGG GAAACACAGG CAGCACATGG GAACTGGATG 
CCATTCCAGC CAACATCAGT AATGCCCCAG ACATCTGAGT CACTGACTAC TGGTGGCCCT 
CACCCTCTCA GGACCAGCAC TGAGTTAGCA CAAGGCATTT TCAGCACAAC TACTACCTCA 
GCGCCACAAA GCACTTTGGC AAACACAAAC ATCATACCAC GTCCACAGCA CCTCAGAATT 
GGCCACCCTC CACTAGCTCA GAAAGCCTCT GGAATTTCCC ATATGCCTCT GAAAGAAAAC 
ATGATAGCCC AATCAGGTAC CACCACTGTA TCATTCACTA ACGTGCCAGA TTCCCACTGC 
TGGCATAACA AGGAGCTGCA TTTAAGTGGT AGTACATGGG CAGCACTGGG ATGTACTGAC 
TGCACTTGCC AGGATGGAAA TATATCTTGT GAAAAAAGGA TATGCACCCC AAACTGTTCA 
CACCCCGTCA TGTATCCTGA CAGCTGCTGC CCTATGTGTG ACGGATGCCT TTTTGAGGGA 
GTTTCCAGGG TAGATGGAGA GCTTTTCCCA AATTCTCCAG ACAATTGCAC AATCTGCATC 
TGCCTGGCTG GAAATGTCAC ATGTATCCCT CCTGTGTGCC CTCCTGTTAC ATGCACTGAC 
CCCTTCCTAT CAGACTGCTG CCTCAGATGC CCAGATGGAT GTGAATTCCA AGATCATTTT 
TATCCCCATG GATCCAAATT TAGCAGGGAT GATAATGGAT GCACCTCTTG TTTATGTCAG 
AACGGCGCTG TTGAATGTTC GTTTATTCCA TGTCCCAGTC TAGAATGTCC CAGAGAGAAC 
TGGGTGCTGG AGGCCGGCCA GTGTTGCTTT AAATGCCAGG AACCCATGCA AAGGACAGGG 
TGTCCTTTTG ATGATAATGG AATTGAAATT CCTGTTGGGC AGATCTGGTC GCCGGGAGAT 
CCCTGTGCAA TCTGCATTTG TCAGGCAGAC AGCTCCATCG TGTGCAAGAA AACAGACTGC 
GTGGAGACCT GTCCACATCC AATCACAGTG CCAGGGCAAT GCTGCCCAGA TTGCTCAGCG 
GGTTGTTCTT ATGGTAGAAG AACATTCAGG AACAATGAAA GCTTCCCCTC TAATTCAGAC 
CCATGTCTCA CTTGCATTTG TCTGATGGGC ACTGTAGCCT GTTCTCCAAT TGAGTGTGCA 
GTAAACTGCA CGTACCCTTT CCACGATGAA GGAGAGTGCT GCCCTGTTTG TCGAGATTGC 
ACATATGATG GCAGAAAAGT TCTGAATGAA CAGACTTTCT ACCTCGAGAG TGATCCTTGC 
ACTCAGTGCA CTTGTCAGCT TGGAGAGGTG CATTGTGAGG CAATCGTGTG CTCTGCTTCC 
TGCTCACATC CTTATGTCTT TCCTGGGGAA TGTTGCTCCT CTTGTGAAGA GTGTCTCTTT 
GAAGGCCATC TTTTAGAGAA TGGAGGCTCC TACATATTAA AGGTGGATCC CTGCATTGTG 
TGTCATTGTT CTGGTGGAAA TGTTCAATGT GAGCAAAGGG ATAATTCCTG TACTCCATGT 
GTGCAGAAGA CACAGGATTG TCTAAATGAA GTGCCAGATA TTTTTCATTA TAATCAGCTG 
CAAATCATGC CTGCGGATCT ACATCAGTCT GCTGGATCCC ACAGTCCAAA TATCGCACTG 
AAGCTACTGA ATGATGCAGT GATTTCCCAA CCCACCTATA TTCCTTTTTT CCAAAGATTA 
GTATCCAGGC GAAATACTGA GATCTTAGGA ACAATTTCAG CTGGTCCAAC TACTGTCCTT 
CATGGAAAAA CACCTGCAGA CTCAACTCCT CCTGGCTATA ATAAGGATTA TTTAACTACA 
AACCCTGTTG ACCCTTTGCC AAATGCACAC ACAACAATCA CCCACACAGG AGAAATAAAG 
TCTGTTTCAG CATCAGAGCC ATTCAGAAAC ATTATTATGC CTTCTTCAAA ATTGCCTCAA 
AGAGAAGCTA CATCATCAAA CTCATCTCCA CAACCTGAAG AATATTTGCA TGAATCTTTT 
CTGGGTTTGC ATACACAAAA GTTATCTACA CCTACCTCCT CTTTAGGCAT TCCTAATAAG 
TCTTTCCCTT CAGCAGTTAT TTCTACCTCA ACACTAATGC CTCAAATGTT TCCTTTTTCT 
GATATGTCTT CTGGTCCATC CAAACTAACT TCTGACAGCG CTGCCCTTCA TCCACCTGCT 
TGGAATGCAG ATAGTTCTGT TCAGTCACCA GCACATGATT TTATTTCTTC TTTCACCCAT 
ACACCGGACT CACATTCTTT ATTTACCCAC TCACAAAGAC CCCTCTCTTC TGGCACTTCT 
GATATATCAT CTATCCATGC AGATACCACT CAATTCTCTT TGTTAGCCAA AAACACTCTA 
GGCTATACCT CGGCCCCTGC ACCCCATGCT GCTCACTTCC CAACCTCTGC AAACATCATC 
CCGAGTGATT CTGGGATTCC TCTCATGCAG CTAAACCACA GAGAGTCAGA GAGTGATGAA 
CTGGGGAATG GACACGCAAT TGGATGTTCT TTAAAAGGTC TTGAATTTTC CAGTGGCTCC 
ATCTTTGTTG CTGACATGGA CTACTGCCTG CCCTGTGAAT GTTTGAATGG AGAGATCTAC 
TGTTCACACC AAGATTTATT CCCCAGTGGA CTGTGTTGCC AGAGCTGTAG CCACCAGGGT 
GTTGATTCTT GTGCAGGGAA AATAAACCAG GTCCATTCAG AAAGTATGAG GAGTCAGAAC 
CTTTGCAATT GCAGAAATCT CAGTCTTCAC TGTGACTCTT GCTCGTTCAG AGAAGAATGC 
AGAAGAAATG GCTCTACACA AACCGCCAAG GAAGAACAAC TACCTGAGAA CATCCACTGG 
GCTCCACTTA TATTTGAAGG GGATGTTGAG CCTCAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948816.2
CDS	618..4619
Translation	MLPFWLGMSCLLLLISETGGRVYKGRKKPPALTAQRLRSGPHVCFSGMGAGCCPGWTVSPGTGQCLQPLCSFGCASGFCIAPNVCSCRDGHQGVTCADEFSSEHEKTLEDKGTPLTCLSALCDQNCRLVNGVPSCSCFHGYSLGKDGRTCYDVDECSRPQASSLCQQQCKNSIGSYRCLCFYGYQISPNGRSCIPTKNLNFIAGIAAPSPCGEYGCELMCNDGGCEHVSRVCPVGFRMAETVNGVTCTDIDECEASSCEGTCINTEGGFVCDCGPGMKLSTDRTSCYDINECRRPGTSHLCKHFCHNTHGSFFCSCRAGFLLDPDKVSCIDVDECLENATICPLGSCVNTLGSYFCAEIPAGMQTTGLPWETQAAHGNWMPFQPTSVMPQTSESLTTGGPHPLRTSTELAQGIFSTTTTSAPQSTLANTNIIPRPQHLRIGHPPLAQKASGISHMPLKENMIAQSGTTTVSFTNVPDSHCWHNKELHLSGSTWAALGCTDCTCQDGNISCEKRICTPNCSHPVMYPDSCCPMCDGCLFEGVSRVDGELFPNSPDNCTICICLAGNVTCIPPVCPPVTCTDPFLSDCCLRCPDGCEFQDHFYPHGSKFSRDDNGCTSCLCQNGAVECSFIPCPSLECPRENWVLEAGQCCFKCQEPMQRTGCPFDDNGIEIPVGQIWSPGDPCAICICQADSSIVCKKTDCVETCPHPITVPGQCCPDCSAGCSYGRRTFRNNESFPSNSDPCLTCICLMGTVACSPIECAVNCTYPFHDEGECCPVCRDCTYDGRKVLNEQTFYLESDPCTQCTCQLGEVHCEAIVCSASCSHPYVFPGECCSSCEECLFEGHLLENGGSYILKVDPCIVCHCSGGNVQCEQRDNSCTPCVQKTQDCLNEVPDIFHYNQLQIMPADLHQSAGSHSPNIALKLLNDAVISQPTYIPFFQRLVSRRNTEILGTISAGPTTVLHGKTPADSTPPGYNKDYLTTNPVDPLPNAHTTITHTGEIKSVSASEPFRNIIMPSSKLPQREATSSNSSPQPEEYLHESFLGLHTQKLSTPTSSLGIPNKSFPSAVISTSTLMPQMFPFSDMSSGPSKLTSDSAALHPPAWNADSSVQSPAHDFISSFTHTPDSHSLFTHSQRPLSSGTSDISSIHADTTQFSLLAKNTLGYTSAPAPHAAHFPTSANIIPSDSGIPLMQLNHRESESDELGNGHAIGCSLKGLEFSSGSIFVADMDYCLPCECLNGEIYCSHQDLFPSGLCCQSCSHQGVDSCAGKINQVHSESMRSQNLCNCRNLSLHCDSCSFREECRRNGSTQTAKEEQLPENIHWAPLIFEGDVEPQR

Target ORF information:

RefSeq Version	XM_018093327.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093327.2

ORF Insert Sequence:

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTGC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCAAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATAAAT GAATGTCGGA GGCCAGGAAC ATCCCATCTT 
TGCAAACACT TCTGCCACAA TACTCATGGA AGTTTCTTCT GTTCTTGCCG GGCTGGGTTT 
CTGCTTGATC CAGATAAGGT GTCATGTATT GATGTGGATG AATGTTTAGA AAATGCCACC 
ATATGCCCTC TGGGAAGCTG TGTGAACACA CTGGGGTCCT ATTTCTGTGC AGAGATTCCA 
GCGGGAATGC AGACCACAGG CCTCCCTTGG GAAACACAGG CAGCACATGG GAACTGGATG 
CCATTCCAGC CAACATCAGT AATGCCCCAG ACATCTGAGT CACTGACTAC TGGTGGCCCT 
CACCCTCTCA GGACCAGCAC TGAGTTAGCA CAAGGCATTT TCAGCACAAC TACTACCTCA 
GCGCCACAAA GCACTTTGGC AAACACAAAC ATCATACCAC GTCCACAGCA CCTCAGAATT 
GGCCACCCTC CACTAGCTCA GAAAGCCTCT GGAATTTCCC ATATGCCTCT GAAAGAAAAC 
ATGATAGCCC AATCAGGTAC CACCACTGTA TCATTCACTA ACGTGCCAGA TTCCCACTGC 
TGGCATAACA AGGAGCTGCA TTTAAGTGGT AGTACATGGG CAGCACTGGG ATGTACTGAC 
TGCACTTGCC AGGATGGAAA TATATCTTGT GAAAAAAGGA TATGCACCCC AAACTGTTCA 
CACCCCGTCA TGTATCCTGA CAGCTGCTGC CCTATGTGTG ACGGATGCCT TTTTGAGGGA 
GTTTCCAGGG TAGATGGAGA GCTTTTCCCA AATTCTCCAG ACAATTGCAC AATCTGCATC 
TGCCTGGCTG GAAATGTCAC ATGTATCCCT CCTGTGTGCC CTCCTGTTAC ATGCACTGAC 
CCCTTCCTAT CAGACTGCTG CCTCAGATGC CCAGATGGAT GTGAATTCCA AGATCATTTT 
TATCCCCATG GATCCAAATT TAGCAGGGAT GATAATGGAT GCACCTCTTG TTTATGTCAG 
AACGGCGCTG TTGAATGTTC GTTTATTCCA TGTCCCAGTC TAGAATGTCC CAGAGAGAAC 
TGGGTGCTGG AGGCCGGCCA GTGTTGCTTT AAATGCCAGG AACCCATGCA AAGGACAGGG 
TGTCCTTTTG ATGATAATGG AATTGAAATT CCTGTTGGGC AGATCTGGTC GCCGGGAGAT 
CCCTGTGCAA TCTGCATTTG TCAGGCAGAC AGCTCCATCG TGTGCAAGAA AACAGACTGC 
GTGGAGACCT GTCCACATCC AATCACAGTG CCAGGGCAAT GCTGCCCAGA TTGCTCAGCG 
GGTTGTTCTT ATGGTAGAAG AACATTCAGG AACAATGAAA GCTTCCCCTC TAATTCAGAC 
CCATGTCTCA CTTGCATTTG TCTGATGGGC ACTGTAGCCT GTTCTCCAAT TGAGTGTGCA 
GTAAACTGCA CGTACCCTTT CCACGATGAA GGAGAGTGCT GCCCTGTTTG TCGAGATTGC 
ACATATGATG GCAGAAAAGT TCTGAATGAA CAGACTTTCT ACCTCGAGAG TGATCCTTGC 
ACTCAGTGCA CTTGTCAGCT TGGAGAGGTG CATTGTGAGG CAATCGTGTG CTCTGCTTCC 
TGCTCACATC CTTATGTCTT TCCTGGGGAA TGTTGCTCCT CTTGTGAAGA GTGTCTCTTT 
GAAGGCCATC TTTTAGAGAA TGGAGGCTCC TACATATTAA AGGTGGATCC CTGCATTGTG 
TGTCATTGTT CTGGTGGAAA TGTTCAATGT GAGCAAAGGG ATAATTCCTG TACTCCATGT 
GTGCAGAAGA CACAGGATTG TCTAAATGAA GTGCCAGATA TTTTTCATTA TAATCAGCTG 
CAAATCATGC CTGCGGATCT ACATCAGTCT GCTGGATCCC ACAGTCCAAA TATCGCACTG 
AAGCTACTGA ATGATGCAGT GATTTCCCAA CCCACCTATA TTCCTTTTTT CCAAAGATTA 
GTATCCAGGC GAAATACTGA GATCTTAGGA ACAATTTCAG CTGGTCCAAC TACTGTCCTT 
CATGGAAAAA CACCTGCAGA CTCAACTCCT CCTGGCTATA ATAAGGATTA TTTAACTACA 
AACCCTGTTG ACCCTTTGCC AAATGCACAC ACAACAATCA CCCACACAGG AGAAATAAAG 
TCTGTTTCAG CATCAGAGCC ATTCAGAAAC ATTATTATGC CTTCTTCAAA ATTGCCTCAA 
AGAGAAGCTA CATCATCAAA CTCATCTCCA CAACCTGAAG AATATTTGCA TGAATCTTTT 
CTGGGTTTGC ATACACAAAA GTTATCTACA CCTACCTCCT CTTTAGGCAT TCCTAATAAG 
TCTTTCCCTT CAGCAGTTAT TTCTACCTCA ACACTAATGC CTCAAATGTT TCCTTTTTCT 
GATATGTCTT CTGGTCCATC CAAACTAACT TCTGACAGCG CTGCCCTTCA TCCACCTGCT 
TGGAATGCAG ATAGTTCTGT TCAGTCACCA GCACATGATT TTATTTCTTC TTTCACCCAT 
ACACCGGACT CACATTCTTT ATTTACCCAC TCACAAAGAC CCCTCTCTTC TGGCACTTCT 
GATATATCAT CTATCCATGC AGATACCACT CAATTCTCTT TGTTAGCCAA AAACACTCTA 
GGCTATACCT CGGCCCCTGC ACCCCATGCT GCTCACTTCC CAACCTCTGC AAACATCATC 
CCGAGTGATT CTGGGATTCC TCTCATGCAG CTAAACCACA GAGAGTCAGA GAGTGATGAA 
CTGGGGAATG GACACGCAAT TGGATGTTCT TTAAAAGGTC TTGAATTTTC CAGTGGCTCC 
ATCTTTGTTG CTGACATGGA CTACTGCCTG CCCTGTGAAT GTTTGAATGG AGAGATCTAC 
TGTTCACACC AAGATTTATT CCCCAGTGGA CTGTGTTGCC AGAGCTGTAG CCACCAGGGT 
GTTGATTCTT GTGCAGGGAA AATAAACCAG GTCCATTCAG AAAGTATGAG GAGTCAGAAC 
CTTTGCAATT GCAGAAATCT CAGTCTTCAC TGTGACTCTT GCTCGTTCAG AGAAGAATGC 
AGAAGAAATG GCTCTACACA AACCGCCAAG GAAGAACAAC TACCTGAGAA CATCCACTGG 
GCTCCACTTA TATTTGAAGG GGATGTTGAG CCTCAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwce ( XM_031900558.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756418.1 Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X4, mRNA.

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CloneID	OXa56585
Clone ID Related Accession (Same CDS sequence)	XM_031900558.1
Accession Version	XM_031900558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor C and EGF domain-containing protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTTT CTCAGGGATG GGAGCGGGTT GCTGCCCTGG CTGGACAGTA TCTCCTGGCA 
CAGGGCAGTG TCTTCAGCCC CTTTGTTCAT TTGGCTGCGC AAGTGGGTTC TGCATTGCCC 
CAAATGTATG TTCCTGCCGA GATGGGCATC AAGGTGTCAC TTGTGCAGAT GAATTTTCCA 
GTGAACATGA AAAGACTTTG GAAGACAAAG AACTGTAGAT TGGTGAATGG GGTTCCATCA 
TGTAGCTGTT TCCATGGATA CAGTCTTGGG AAAGATGGCA GAACCTGCTA TGATGTTGAT 
GAATGTTCCA GGCCACAGGC ATCCAGCCTC TGTCAACAGC AATGCAAGAA CAGTATTGGC 
AGCTATAGAT GCCTCTGTTT CTATGGGTAT CAAATATCAC CCAATGGTCG CTCTTGTATA 
CCAACCAAGA ACCTAAACTT TATCGCAGGC ATTGCAGCTC CTTCCCCTTG TGGAGAATAC 
GGCTGTGAAT TAATGTGCAA TGATGGCGGC TGTGAGCACG TGTCAAGGGT GTGTCCTGTT 
GGATTCCGAA TGGCAGAGAC TGTAAATGGA GTGACCTGCA CAGATATTGA TGAATGTGAG 
GCCTCTTCAT GTGAAGGAAC CTGCATAAAT ACAGAGGGAG GATTCGTGTG TGACTGTGGG 
CCAGGCATGA AGCTATCTAC TGACAGGACG AGCTGCTACG ACATTGATGA ATGTTCCTCT 
AAAAGATCCC TGTGCCAACA AAAATGCAAG AACCTTCAGG GCAGCTACAG GTGTTTGTGT 
GCGACGGGGT TCGTCCTTCA GAGCAATGGA CACACCTGTG CAGACATAAA TGAATGTCGG 
AGGCCAGGAA CATCCCATCT TTGCAAACAC TTCTGCCACA ATACTCATGG AAGTTTCTTC 
TGTTCTTGCC GGGCTGGGTT TCTGCTTGAT CCAGATAAGG TGTCATGTAT TGATGTGGAT 
GAATGTTTAG AAAATGCCAC CATATGCCCT CTGGGAAGCT GTGTGAACAC ACTGGGGTCC 
TATTTCTGTG CAGAGATTCC AGCGGGAATG CAGACCACAG GCCTCCCTTG GGAAACACAG 
GCAGCACATG GGAACTGGAT GCCATTCCAG CCAACATCAG TAATGCCCCA GACATCTGAG 
TCACTGACTA CTGGTGGCCC TCACCCTCTC AGGACCAGCA CTGAGTTAGC ACAAGGCATT 
TTCAGCACAA CTACTACCTC AGCGCCACAA AGCACTTTGG CAAACACAAA CATCATACCA 
CGTCCACAGC ACCTCAGAAT TGGCCACCCT CCACTAGCTC AGAAAGCCTC TGGAATTTCC 
CATATGCCTC TGAAAGAAAA CATGATAGCC CAATCAGGTA CCACCACTGT ATCATTCACT 
AACGTGCCAG ATTCCCACTG CTGGCATAAC AAGGAGCTGC ATTTAAGTGG TAGTACATGG 
GCAGCACTGG GATGTACTGA CTGCACTTGC CAGGATGGAA ATATATCTTG TGAAAAAAGG 
ATATGCACCC CAAACTGTTC ACACCCCGTC ATGTATCCTG ACAGCTGCTG CCCTATGTGT 
GACGGATGCC TTTTTGAGGG AGTTTCCAGG GTAGATGGAG AGCTTTTCCC AAATTCTCCA 
GACAATTGCA CAATCTGCAT CTGCCTGGCT GGAAATGTCA CATGTATCCC TCCTGTGTGC 
CCTCCTGTTA CATGCACTGA CCCCTTCCTA TCAGACTGCT GCCTCAGATG CCCAGATGGA 
TGTGAATTCC AAGATCATTT TTATCCCCAT GGATCCAAAT TTAGCAGGGA TGATAATGGA 
TGCACCTCTT GTTTATGTCA GAACGGCGCT GTTGAATGTT CGTTTATTCC ATGTCCCAGT 
CTAGAATGTC CCAGAGAGAA CTGGGTGCTG GAGGCCGGCC AGTGTTGCTT TAAATGCCAG 
GAACCCATGC AAAGGACAGG GTGTCCTTTT GATGATAATG GAATTGAAAT TCCTGTTGGG 
CAGATCTGGT CGCCGGGAGA TCCCTGTGCA ATCTGCATTT GTCAGGCAGA CAGCTCCATC 
GTGTGCAAGA AAACAGACTG CGTGGAGACC TGTCCACATC CAATCACAGT GCCAGGGCAA 
TGCTGCCCAG ATTGCTCAGC GGGTTGTTCT TATGGTAGAA GAACATTCAG GAACAATGAA 
AGCTTCCCCT CTAATTCAGA CCCATGTCTC ACTTGCATTT GTCTGATGGG CACTGTAGCC 
TGTTCTCCAA TTGAGTGTGC AGTAAACTGC ACGTACCCTT TCCACGATGA AGGAGAGTGC 
TGCCCTGTTT GTCGAGATTG CACATATGAT GGCAGAAAAG TTCTGAATGA ACAGACTTTC 
TACCTCGAGA GTGATCCTTG CACTCAGTGC ACTTGTCAGC TTGGAGAGGT GCATTGTGAG 
GCAATCGTGT GCTCTGCTTC CTGCTCACAT CCTTATGTCT TTCCTGGGGA ATGTTGCTCC 
TCTTGTGAAG AGTGTCTCTT TGAAGGCCAT CTTTTAGAGA ATGGAGGCTC CTACATATTA 
AAGGTGGATC CCTGCATTGT GTGTCATTGT TCTGGTGGAA ATGTTCAATG TGAGCAAAGG 
GATAATTCCT GTACTCCATG TGTGCAGAAG ACACAGGATT GTCTAAATGA AGTGCCAGAT 
ATTTTTCATT ATAATCAGCT GCAAATCATG CCTGCGGATC TACATCAGTC TGCTGGATCC 
CACAGTCCAA ATATCGCACT GAAGCTACTG AATGATGCAG TGATTTCCCA ACCCACCTAT 
ATTCCTTTTT TCCAAAGATT AGTATCCAGG CGAAATACTG AGATCTTAGG AACAATTTCA 
GCTGGTCCAA CTACTGTCCT TCATGGAAAA ACACCTGCAG ACTCAACTCC TCCTGGCTAT 
AATAAGGATT ATTTAACTAC AAACCCTGTT GACCCTTTGC CAAATGCACA CACAACAATC 
ACCCACACAG GAGAAATAAA GTCTGTTTCA GCATCAGAGC CATTCAGAAA CATTATTATG 
CCTTCTTCAA AATTGCCTCA AAGAGAAGCT ACATCATCAA ACTCATCTCC ACAACCTGAA 
GAATATTTGC ATGAATCTTT TCTGGGTTTG CATACACAAA AGTTATCTAC ACCTACCTCC 
TCTTTAGGCA TTCCTAATAA GTCTTTCCCT TCAGCAGTTA TTTCTACCTC AACACTAATG 
CCTCAAATGT TTCCTTTTTC TGATATGTCT TCTGGTCCAT CCAAACTAAC TTCTGACAGC 
GCTGCCCTTC ATCCACCTGC TTGGAATGCA GATAGTTCTG TTCAGTCACC AGCACATGAT 
TTTATTTCTT CTTTCACCCA TACACCGGAC TCACATTCTT TATTTACCCA CTCACAAAGA 
CCCCTCTCTT CTGGCACTTC TGATATATCA TCTATCCATG CAGATACCAC TCAATTCTCT 
TTGTTAGCCA AAAACACTCT AGGCTATACC TCGGCCCCTG CACCCCATGC TGCTCACTTC 
CCAACCTCTG CAAACATCAT CCCGAGTGAT TCTGGGATTC CTCTCATGCA GCTAAACCAC 
AGAGAGTCAG AGAGTGATGA ACTGGGGAAT GGACACGCAA TTGGATGTTC TTTAAAAGGT 
CTTGAATTTT CCAGTGGCTC CATCTTTGTT GCTGACATGG ACTACTGCCT GCCCTGTGAA 
TGTTTGAATG GAGAGATCTA CTGTTCACAC CAAGATTTAT TCCCCAGTGG ACTGTGTTGC 
CAGAGCTGTA GCCACCAGGG TGTTGATTCT TGTGCAGGGA AAATAAACCA GGTCCATTCA 
GAAAGTATGA GGAGTCAGAA CCTTTGCAAT TGCAGAAATC TCAGTCTTCA CTGTGACTCT 
TGCTCGTTCA GAGAAGAATG CAGAAGAAAT GGCTCTACAC AAACCGCCAA GGAAGAACAA 
CTACCTGAGA ACATCCACTG GGCTCCACTT ATATTTGAAG GGGATGTTGA GCCTCAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756418.1
CDS	107..4069
Translation	MFVSQGWERVAALAGQYLLAQGSVFSPFVHLAAQVGSALPQMYVPAEMGIKVSLVQMNFPVNMKRLWKTKNCRLVNGVPSCSCFHGYSLGKDGRTCYDVDECSRPQASSLCQQQCKNSIGSYRCLCFYGYQISPNGRSCIPTKNLNFIAGIAAPSPCGEYGCELMCNDGGCEHVSRVCPVGFRMAETVNGVTCTDIDECEASSCEGTCINTEGGFVCDCGPGMKLSTDRTSCYDIDECSSKRSLCQQKCKNLQGSYRCLCATGFVLQSNGHTCADINECRRPGTSHLCKHFCHNTHGSFFCSCRAGFLLDPDKVSCIDVDECLENATICPLGSCVNTLGSYFCAEIPAGMQTTGLPWETQAAHGNWMPFQPTSVMPQTSESLTTGGPHPLRTSTELAQGIFSTTTTSAPQSTLANTNIIPRPQHLRIGHPPLAQKASGISHMPLKENMIAQSGTTTVSFTNVPDSHCWHNKELHLSGSTWAALGCTDCTCQDGNISCEKRICTPNCSHPVMYPDSCCPMCDGCLFEGVSRVDGELFPNSPDNCTICICLAGNVTCIPPVCPPVTCTDPFLSDCCLRCPDGCEFQDHFYPHGSKFSRDDNGCTSCLCQNGAVECSFIPCPSLECPRENWVLEAGQCCFKCQEPMQRTGCPFDDNGIEIPVGQIWSPGDPCAICICQADSSIVCKKTDCVETCPHPITVPGQCCPDCSAGCSYGRRTFRNNESFPSNSDPCLTCICLMGTVACSPIECAVNCTYPFHDEGECCPVCRDCTYDGRKVLNEQTFYLESDPCTQCTCQLGEVHCEAIVCSASCSHPYVFPGECCSSCEECLFEGHLLENGGSYILKVDPCIVCHCSGGNVQCEQRDNSCTPCVQKTQDCLNEVPDIFHYNQLQIMPADLHQSAGSHSPNIALKLLNDAVISQPTYIPFFQRLVSRRNTEILGTISAGPTTVLHGKTPADSTPPGYNKDYLTTNPVDPLPNAHTTITHTGEIKSVSASEPFRNIIMPSSKLPQREATSSNSSPQPEEYLHESFLGLHTQKLSTPTSSLGIPNKSFPSAVISTSTLMPQMFPFSDMSSGPSKLTSDSAALHPPAWNADSSVQSPAHDFISSFTHTPDSHSLFTHSQRPLSSGTSDISSIHADTTQFSLLAKNTLGYTSAPAPHAAHFPTSANIIPSDSGIPLMQLNHRESESDELGNGHAIGCSLKGLEFSSGSIFVADMDYCLPCECLNGEIYCSHQDLFPSGLCCQSCSHQGVDSCAGKINQVHSESMRSQNLCNCRNLSLHCDSCSFREECRRNGSTQTAKEEQLPENIHWAPLIFEGDVEPQR

Target ORF information:

RefSeq Version	XM_031900558.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900558.1

ORF Insert Sequence:

ATGTTTGTTT CTCAGGGATG GGAGCGGGTT GCTGCCCTGG CTGGACAGTA TCTCCTGGCA 
CAGGGCAGTG TCTTCAGCCC CTTTGTTCAT TTGGCTGCGC AAGTGGGTTC TGCATTGCCC 
CAAATGTATG TTCCTGCCGA GATGGGCATC AAGGTGTCAC TTGTGCAGAT GAATTTTCCA 
GTGAACATGA AAAGACTTTG GAAGACAAAG AACTGTAGAT TGGTGAATGG GGTTCCATCA 
TGTAGCTGTT TCCATGGATA CAGTCTTGGG AAAGATGGCA GAACCTGCTA TGATGTTGAT 
GAATGTTCCA GGCCACAGGC ATCCAGCCTC TGTCAACAGC AATGCAAGAA CAGTATTGGC 
AGCTATAGAT GCCTCTGTTT CTATGGGTAT CAAATATCAC CCAATGGTCG CTCTTGTATA 
CCAACCAAGA ACCTAAACTT TATCGCAGGC ATTGCAGCTC CTTCCCCTTG TGGAGAATAC 
GGCTGTGAAT TAATGTGCAA TGATGGCGGC TGTGAGCACG TGTCAAGGGT GTGTCCTGTT 
GGATTCCGAA TGGCAGAGAC TGTAAATGGA GTGACCTGCA CAGATATTGA TGAATGTGAG 
GCCTCTTCAT GTGAAGGAAC CTGCATAAAT ACAGAGGGAG GATTCGTGTG TGACTGTGGG 
CCAGGCATGA AGCTATCTAC TGACAGGACG AGCTGCTACG ACATTGATGA ATGTTCCTCT 
AAAAGATCCC TGTGCCAACA AAAATGCAAG AACCTTCAGG GCAGCTACAG GTGTTTGTGT 
GCGACGGGGT TCGTCCTTCA GAGCAATGGA CACACCTGTG CAGACATAAA TGAATGTCGG 
AGGCCAGGAA CATCCCATCT TTGCAAACAC TTCTGCCACA ATACTCATGG AAGTTTCTTC 
TGTTCTTGCC GGGCTGGGTT TCTGCTTGAT CCAGATAAGG TGTCATGTAT TGATGTGGAT 
GAATGTTTAG AAAATGCCAC CATATGCCCT CTGGGAAGCT GTGTGAACAC ACTGGGGTCC 
TATTTCTGTG CAGAGATTCC AGCGGGAATG CAGACCACAG GCCTCCCTTG GGAAACACAG 
GCAGCACATG GGAACTGGAT GCCATTCCAG CCAACATCAG TAATGCCCCA GACATCTGAG 
TCACTGACTA CTGGTGGCCC TCACCCTCTC AGGACCAGCA CTGAGTTAGC ACAAGGCATT 
TTCAGCACAA CTACTACCTC AGCGCCACAA AGCACTTTGG CAAACACAAA CATCATACCA 
CGTCCACAGC ACCTCAGAAT TGGCCACCCT CCACTAGCTC AGAAAGCCTC TGGAATTTCC 
CATATGCCTC TGAAAGAAAA CATGATAGCC CAATCAGGTA CCACCACTGT ATCATTCACT 
AACGTGCCAG ATTCCCACTG CTGGCATAAC AAGGAGCTGC ATTTAAGTGG TAGTACATGG 
GCAGCACTGG GATGTACTGA CTGCACTTGC CAGGATGGAA ATATATCTTG TGAAAAAAGG 
ATATGCACCC CAAACTGTTC ACACCCCGTC ATGTATCCTG ACAGCTGCTG CCCTATGTGT 
GACGGATGCC TTTTTGAGGG AGTTTCCAGG GTAGATGGAG AGCTTTTCCC AAATTCTCCA 
GACAATTGCA CAATCTGCAT CTGCCTGGCT GGAAATGTCA CATGTATCCC TCCTGTGTGC 
CCTCCTGTTA CATGCACTGA CCCCTTCCTA TCAGACTGCT GCCTCAGATG CCCAGATGGA 
TGTGAATTCC AAGATCATTT TTATCCCCAT GGATCCAAAT TTAGCAGGGA TGATAATGGA 
TGCACCTCTT GTTTATGTCA GAACGGCGCT GTTGAATGTT CGTTTATTCC ATGTCCCAGT 
CTAGAATGTC CCAGAGAGAA CTGGGTGCTG GAGGCCGGCC AGTGTTGCTT TAAATGCCAG 
GAACCCATGC AAAGGACAGG GTGTCCTTTT GATGATAATG GAATTGAAAT TCCTGTTGGG 
CAGATCTGGT CGCCGGGAGA TCCCTGTGCA ATCTGCATTT GTCAGGCAGA CAGCTCCATC 
GTGTGCAAGA AAACAGACTG CGTGGAGACC TGTCCACATC CAATCACAGT GCCAGGGCAA 
TGCTGCCCAG ATTGCTCAGC GGGTTGTTCT TATGGTAGAA GAACATTCAG GAACAATGAA 
AGCTTCCCCT CTAATTCAGA CCCATGTCTC ACTTGCATTT GTCTGATGGG CACTGTAGCC 
TGTTCTCCAA TTGAGTGTGC AGTAAACTGC ACGTACCCTT TCCACGATGA AGGAGAGTGC 
TGCCCTGTTT GTCGAGATTG CACATATGAT GGCAGAAAAG TTCTGAATGA ACAGACTTTC 
TACCTCGAGA GTGATCCTTG CACTCAGTGC ACTTGTCAGC TTGGAGAGGT GCATTGTGAG 
GCAATCGTGT GCTCTGCTTC CTGCTCACAT CCTTATGTCT TTCCTGGGGA ATGTTGCTCC 
TCTTGTGAAG AGTGTCTCTT TGAAGGCCAT CTTTTAGAGA ATGGAGGCTC CTACATATTA 
AAGGTGGATC CCTGCATTGT GTGTCATTGT TCTGGTGGAA ATGTTCAATG TGAGCAAAGG 
GATAATTCCT GTACTCCATG TGTGCAGAAG ACACAGGATT GTCTAAATGA AGTGCCAGAT 
ATTTTTCATT ATAATCAGCT GCAAATCATG CCTGCGGATC TACATCAGTC TGCTGGATCC 
CACAGTCCAA ATATCGCACT GAAGCTACTG AATGATGCAG TGATTTCCCA ACCCACCTAT 
ATTCCTTTTT TCCAAAGATT AGTATCCAGG CGAAATACTG AGATCTTAGG AACAATTTCA 
GCTGGTCCAA CTACTGTCCT TCATGGAAAA ACACCTGCAG ACTCAACTCC TCCTGGCTAT 
AATAAGGATT ATTTAACTAC AAACCCTGTT GACCCTTTGC CAAATGCACA CACAACAATC 
ACCCACACAG GAGAAATAAA GTCTGTTTCA GCATCAGAGC CATTCAGAAA CATTATTATG 
CCTTCTTCAA AATTGCCTCA AAGAGAAGCT ACATCATCAA ACTCATCTCC ACAACCTGAA 
GAATATTTGC ATGAATCTTT TCTGGGTTTG CATACACAAA AGTTATCTAC ACCTACCTCC 
TCTTTAGGCA TTCCTAATAA GTCTTTCCCT TCAGCAGTTA TTTCTACCTC AACACTAATG 
CCTCAAATGT TTCCTTTTTC TGATATGTCT TCTGGTCCAT CCAAACTAAC TTCTGACAGC 
GCTGCCCTTC ATCCACCTGC TTGGAATGCA GATAGTTCTG TTCAGTCACC AGCACATGAT 
TTTATTTCTT CTTTCACCCA TACACCGGAC TCACATTCTT TATTTACCCA CTCACAAAGA 
CCCCTCTCTT CTGGCACTTC TGATATATCA TCTATCCATG CAGATACCAC TCAATTCTCT 
TTGTTAGCCA AAAACACTCT AGGCTATACC TCGGCCCCTG CACCCCATGC TGCTCACTTC 
CCAACCTCTG CAAACATCAT CCCGAGTGAT TCTGGGATTC CTCTCATGCA GCTAAACCAC 
AGAGAGTCAG AGAGTGATGA ACTGGGGAAT GGACACGCAA TTGGATGTTC TTTAAAAGGT 
CTTGAATTTT CCAGTGGCTC CATCTTTGTT GCTGACATGG ACTACTGCCT GCCCTGTGAA 
TGTTTGAATG GAGAGATCTA CTGTTCACAC CAAGATTTAT TCCCCAGTGG ACTGTGTTGC 
CAGAGCTGTA GCCACCAGGG TGTTGATTCT TGTGCAGGGA AAATAAACCA GGTCCATTCA 
GAAAGTATGA GGAGTCAGAA CCTTTGCAAT TGCAGAAATC TCAGTCTTCA CTGTGACTCT 
TGCTCGTTCA GAGAAGAATG CAGAAGAAAT GGCTCTACAC AAACCGCCAA GGAAGAACAA 
CTACCTGAGA ACATCCACTG GGCTCCACTT ATATTTGAAG GGGATGTTGA GCCTCAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwce ( XM_031900559.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756419.1 Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X5, mRNA.

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CloneID	OXa56586
Clone ID Related Accession (Same CDS sequence)	XM_031900559.1
Accession Version	XM_031900559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor C and EGF domain-containing protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTGC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCAAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATTGAT GAATGTTCCT CTAAAAGATC CCTGTGCCAA 
CAAAAATGCA AGAACCTTCA GGGCAGCTAC AGGTGTTTGT GTGCGACGGG GTTCGTCCTT 
CAGAGCAATG GACACACCTG TGCAGACATA AATGAATGTC GGAGGCCAGG AACATCCCAT 
CTTTGCAAAC ACTTCTGCCA CAATACTCAT GGAAGTTTCT TCTGTTCTTG CCGGGCTGGG 
TTTCTGCTTG ATCCAGATAA GGTGTCATGT ATTGATGTGG ATGAATGTTT AGAAAATGCC 
ACCATATGCC CTCTGGGAAG CTGTGTGAAC ACACTGGGGT CCTATTTCTG TGCAGAGATT 
CCAGCGGGAA TGCAGACCAC AGGCCTCCCT TGGGAAACAC AGGCAGCACA TGGGAACTGG 
ATGCCATTCC AGCCAACATC AGTAATGCCC CAGACATCTG AGTCACTGAC TACTGGTGGC 
CCTCACCCTC TCAGGACCAG CACTGAGTTA GCACAAGGCA TTTTCAGCAC AACTACTACC 
TCAGCGCCAC AAAGCACTTT GGCAAACACA AACATCATAC CACGTCCACA GCACCTCAGA 
ATTGGCCACC CTCCACTAGC TCAGAAAGCC TCTGGAATTT CCCATATGCC TCTGAAAGAA 
AACATGATAG CCCAATCAGG TACCACCACT GTATCATTCA CTAACGTGCC AGATTCCCAC 
TGCTGGCATA ACAAGGAGCT GCATTTAAGT GGTAGTACAT GGGCAGCACT GGGATGTACT 
GACTGCACTT GCCAGGATGG AAATATATCT TGTGAAAAAA GGATATGCAC CCCAAACTGT 
TCACACCCCG TCATGTATCC TGACAGCTGC TGCCCTATGT GTGACGGATG CCTTTTTGAG 
GGAGTTTCCA GGGTAGATGG AGAGCTTTTC CCAAATTCTC CAGACAATTG CACAATCTGC 
ATCTGCCTGG CTGGAAATGT CACATGTATC CCTCCTGTGT GCCCTCCTGT TACATGCACT 
GACCCCTTCC TATCAGACTG CTGCCTCAGA TGCCCAGATG GATGTGAATT CCAAGATCAT 
TTTTATCCCC ATGGATCCAA ATTTAGCAGG GATGATAATG GATGCACCTC TTGTTTATGT 
CAGAACGGCG CTGTTGAATG TTCGTTTATT CCATGTCCCA GTCTAGAATG TCCCAGAGAG 
AACTGGGTGC TGGAGGCCGG CCAGTGTTGC TTTAAATGCC AGGAACCCAT GCAAAGGACA 
GGGTGTCCTT TTGATGATAA TGGAATTGAA ATTCCTGTTG GGCAGATCTG GTCGCCGGGA 
GATCCCTGTG CAATCTGCAT TTGTCAGGCA GACAGCTCCA TCGTGTGCAA GAAAACAGAC 
TGCGTGGAGA CCTGTCCACA TCCAATCACA GTGCCAGGGC AATGCTGCCC AGATTGCTCA 
GCGGGTTGTT CTTATGGTAG AAGAACATTC AGGAACAATG AAAGCTTCCC CTCTAATTCA 
GACCCATGTC TCACTTGCAT TTGTCTGATG GGCACTGTAG CCTGTTCTCC AATTGAGTGT 
GCAGTAAACT GCACGTACCC TTTCCACGAT GAAGGAGAGT GCTGCCCTGT TTGTCGAGAT 
TGCACATATG ATGGCAGAAA AGTTCTGAAT GAACAGACTT TCTACCTCGA GAGTGATCCT 
TGCACTCAGT GCACTTGTCA GCTTGGAGAG GTGCATTGTG AGGCAATCGT GTGCTCTGCT 
TCCTGCTCAC ATCCTTATGT CTTTCCTGGG GAATGTTGCT CCTCTTGTGA AGAGTGTCTC 
TTTGAAGGCC ATCTTTTAGA GAATGGAGGC TCCTACATAT TAAAGGTGGA TCCCTGCATT 
GTGTGTCATT GTTCTGGTGG AAATGTTCAA TGTGAGCAAA GGGATAATTC CTGTACTCCA 
TGTGTGCAGA AGACACAGGA TTGTCTAAAT GAAGTGCCAG ATATTTTTCA TTATAATCAG 
CTGCAAATCA TGCCTGCGGA TCTACATCAG TCTGCTGGAT CCCACAGTCC AAATATCGCA 
CTGAAGCTAC TGAATGATGC AGTGATTTCC CAACCCACCT ATATTCCTTT TTTCCAAAGA 
TTAGTATCCA GGCGAAATAC TGAGATCTTA GGAACAATTT CAGCTGGTCC AACTACTGTC 
CTTCATGGAA AAACACCTGC AGACTCAACT CCTCCTGGCT ATAATAAGGA TTATTTAACT 
ACAAACCCTG TTGACCCTTT GCCAAATGCA CACACAACAA TCACCCACAC AGGAGAAATA 
AAGTCTGTTT CAGCATCAGA GCCATTCAGA AACATTATTA TGCCTTCTTC AAAATTGCCT 
CAAAGAGAAG CTACATCATC AAACTCATCT CCACAACCTG AAGAATATTT GCATGAATCT 
TTTCTGGGTT TGCATACACA AAAGTTATCT ACACCTACCT CCTCTTTAGG CATTCCTAAT 
AAGTCTTTCC CTTCAGCAGT TATTTCTACC TCAACACTAA TGCCTCAAAT GTTTCCTTTT 
TCTGATATGT CTTCTGGTCC ATCCAAACTA ACTTCTGACA GCGCTGCCCT TCATCCACCT 
GCTTGGAATG CAGATAGTTC TGTTCAGTCA CCAGCACATG ATTTTATTTC TTCTTTCACC 
CATACACCGG ACTCACATTC TTTATTTACC CACTCACAAA GACCCCTCTC TTCTGGCACT 
TCTGATATAT CATCTATCCA TGCAGATACC ACTCAATTCT CTTTGTTAGC CAAAAACACT 
CTAGGCTATA CCTCGGCCCC TGCACCCCAT GCTGCTCACT TCCCAACCTC TGCAAACATC 
ATCCCGAGTG ATTCTGGGAT TCCTCTCATG CAGCTAAACC ACAGAGAGTC AGAGAGTGAT 
GAACTGGGGA ATGGACACGC AATTGGATGT TCTTTAAAAG GTCTTGAATT TTCCAGTGGC 
TCCATCTTTG TTGCTGACAT GGACTACTGC CTGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756419.1
CDS	619..4437
Translation	MLPFWLGMSCLLLLISETGGRVYKGRKKPPALTAQRLRSGPHVCFSGMGAGCCPGWTVSPGTGQCLQPLCSFGCASGFCIAPNVCSCRDGHQGVTCADEFSSEHEKTLEDKGTPLTCLSALCDQNCRLVNGVPSCSCFHGYSLGKDGRTCYDVDECSRPQASSLCQQQCKNSIGSYRCLCFYGYQISPNGRSCIPTKNLNFIAGIAAPSPCGEYGCELMCNDGGCEHVSRVCPVGFRMAETVNGVTCTDIDECEASSCEGTCINTEGGFVCDCGPGMKLSTDRTSCYDIDECSSKRSLCQQKCKNLQGSYRCLCATGFVLQSNGHTCADINECRRPGTSHLCKHFCHNTHGSFFCSCRAGFLLDPDKVSCIDVDECLENATICPLGSCVNTLGSYFCAEIPAGMQTTGLPWETQAAHGNWMPFQPTSVMPQTSESLTTGGPHPLRTSTELAQGIFSTTTTSAPQSTLANTNIIPRPQHLRIGHPPLAQKASGISHMPLKENMIAQSGTTTVSFTNVPDSHCWHNKELHLSGSTWAALGCTDCTCQDGNISCEKRICTPNCSHPVMYPDSCCPMCDGCLFEGVSRVDGELFPNSPDNCTICICLAGNVTCIPPVCPPVTCTDPFLSDCCLRCPDGCEFQDHFYPHGSKFSRDDNGCTSCLCQNGAVECSFIPCPSLECPRENWVLEAGQCCFKCQEPMQRTGCPFDDNGIEIPVGQIWSPGDPCAICICQADSSIVCKKTDCVETCPHPITVPGQCCPDCSAGCSYGRRTFRNNESFPSNSDPCLTCICLMGTVACSPIECAVNCTYPFHDEGECCPVCRDCTYDGRKVLNEQTFYLESDPCTQCTCQLGEVHCEAIVCSASCSHPYVFPGECCSSCEECLFEGHLLENGGSYILKVDPCIVCHCSGGNVQCEQRDNSCTPCVQKTQDCLNEVPDIFHYNQLQIMPADLHQSAGSHSPNIALKLLNDAVISQPTYIPFFQRLVSRRNTEILGTISAGPTTVLHGKTPADSTPPGYNKDYLTTNPVDPLPNAHTTITHTGEIKSVSASEPFRNIIMPSSKLPQREATSSNSSPQPEEYLHESFLGLHTQKLSTPTSSLGIPNKSFPSAVISTSTLMPQMFPFSDMSSGPSKLTSDSAALHPPAWNADSSVQSPAHDFISSFTHTPDSHSLFTHSQRPLSSGTSDISSIHADTTQFSLLAKNTLGYTSAPAPHAAHFPTSANIIPSDSGIPLMQLNHRESESDELGNGHAIGCSLKGLEFSSGSIFVADMDYCLP

Target ORF information:

RefSeq Version	XM_031900559.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor C and EGF domains (vwce), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900559.1

ORF Insert Sequence:

ATGCTCCCAT TTTGGCTGGG GATGTCCTGC TTACTGCTGC TCATTTCTGA AACAGGAGGG 
AGAGTTTATA AGGGGAGGAA GAAACCCCCG GCACTGACAG CGCAAAGGCT CCGATCTGGA 
CCTCATGTTT GTTTCTCAGG GATGGGAGCG GGTTGCTGCC CTGGCTGGAC AGTATCTCCT 
GGCACAGGGC AGTGTCTTCA GCCCCTTTGT TCATTTGGCT GCGCAAGTGG GTTCTGCATT 
GCCCCAAATG TATGTTCCTG CCGAGATGGG CATCAAGGTG TCACTTGTGC AGATGAATTT 
TCCAGTGAAC ATGAAAAGAC TTTGGAAGAC AAAGGTACTC CTCTCACCTG CCTATCTGCT 
TTGTGTGATC AGAACTGTAG ATTGGTGAAT GGGGTTCCAT CATGTAGCTG TTTCCATGGA 
TACAGTCTTG GGAAAGATGG CAGAACCTGC TATGATGTTG ATGAATGTTC CAGGCCACAG 
GCATCCAGCC TCTGTCAACA GCAATGCAAG AACAGTATTG GCAGCTATAG ATGCCTCTGT 
TTCTATGGGT ATCAAATATC ACCCAATGGT CGCTCTTGTA TACCAACCAA GAACCTAAAC 
TTTATCGCAG GCATTGCAGC TCCTTCCCCT TGTGGAGAAT ACGGCTGTGA ATTAATGTGC 
AATGATGGCG GCTGTGAGCA CGTGTCAAGG GTGTGTCCTG TTGGATTCCG AATGGCAGAG 
ACTGTAAATG GAGTGACCTG CACAGATATT GATGAATGTG AGGCCTCTTC ATGTGAAGGA 
ACCTGCATAA ATACAGAGGG AGGATTCGTG TGTGACTGTG GGCCAGGCAT GAAGCTATCT 
ACTGACAGGA CGAGCTGCTA CGACATTGAT GAATGTTCCT CTAAAAGATC CCTGTGCCAA 
CAAAAATGCA AGAACCTTCA GGGCAGCTAC AGGTGTTTGT GTGCGACGGG GTTCGTCCTT 
CAGAGCAATG GACACACCTG TGCAGACATA AATGAATGTC GGAGGCCAGG AACATCCCAT 
CTTTGCAAAC ACTTCTGCCA CAATACTCAT GGAAGTTTCT TCTGTTCTTG CCGGGCTGGG 
TTTCTGCTTG ATCCAGATAA GGTGTCATGT ATTGATGTGG ATGAATGTTT AGAAAATGCC 
ACCATATGCC CTCTGGGAAG CTGTGTGAAC ACACTGGGGT CCTATTTCTG TGCAGAGATT 
CCAGCGGGAA TGCAGACCAC AGGCCTCCCT TGGGAAACAC AGGCAGCACA TGGGAACTGG 
ATGCCATTCC AGCCAACATC AGTAATGCCC CAGACATCTG AGTCACTGAC TACTGGTGGC 
CCTCACCCTC TCAGGACCAG CACTGAGTTA GCACAAGGCA TTTTCAGCAC AACTACTACC 
TCAGCGCCAC AAAGCACTTT GGCAAACACA AACATCATAC CACGTCCACA GCACCTCAGA 
ATTGGCCACC CTCCACTAGC TCAGAAAGCC TCTGGAATTT CCCATATGCC TCTGAAAGAA 
AACATGATAG CCCAATCAGG TACCACCACT GTATCATTCA CTAACGTGCC AGATTCCCAC 
TGCTGGCATA ACAAGGAGCT GCATTTAAGT GGTAGTACAT GGGCAGCACT GGGATGTACT 
GACTGCACTT GCCAGGATGG AAATATATCT TGTGAAAAAA GGATATGCAC CCCAAACTGT 
TCACACCCCG TCATGTATCC TGACAGCTGC TGCCCTATGT GTGACGGATG CCTTTTTGAG 
GGAGTTTCCA GGGTAGATGG AGAGCTTTTC CCAAATTCTC CAGACAATTG CACAATCTGC 
ATCTGCCTGG CTGGAAATGT CACATGTATC CCTCCTGTGT GCCCTCCTGT TACATGCACT 
GACCCCTTCC TATCAGACTG CTGCCTCAGA TGCCCAGATG GATGTGAATT CCAAGATCAT 
TTTTATCCCC ATGGATCCAA ATTTAGCAGG GATGATAATG GATGCACCTC TTGTTTATGT 
CAGAACGGCG CTGTTGAATG TTCGTTTATT CCATGTCCCA GTCTAGAATG TCCCAGAGAG 
AACTGGGTGC TGGAGGCCGG CCAGTGTTGC TTTAAATGCC AGGAACCCAT GCAAAGGACA 
GGGTGTCCTT TTGATGATAA TGGAATTGAA ATTCCTGTTG GGCAGATCTG GTCGCCGGGA 
GATCCCTGTG CAATCTGCAT TTGTCAGGCA GACAGCTCCA TCGTGTGCAA GAAAACAGAC 
TGCGTGGAGA CCTGTCCACA TCCAATCACA GTGCCAGGGC AATGCTGCCC AGATTGCTCA 
GCGGGTTGTT CTTATGGTAG AAGAACATTC AGGAACAATG AAAGCTTCCC CTCTAATTCA 
GACCCATGTC TCACTTGCAT TTGTCTGATG GGCACTGTAG CCTGTTCTCC AATTGAGTGT 
GCAGTAAACT GCACGTACCC TTTCCACGAT GAAGGAGAGT GCTGCCCTGT TTGTCGAGAT 
TGCACATATG ATGGCAGAAA AGTTCTGAAT GAACAGACTT TCTACCTCGA GAGTGATCCT 
TGCACTCAGT GCACTTGTCA GCTTGGAGAG GTGCATTGTG AGGCAATCGT GTGCTCTGCT 
TCCTGCTCAC ATCCTTATGT CTTTCCTGGG GAATGTTGCT CCTCTTGTGA AGAGTGTCTC 
TTTGAAGGCC ATCTTTTAGA GAATGGAGGC TCCTACATAT TAAAGGTGGA TCCCTGCATT 
GTGTGTCATT GTTCTGGTGG AAATGTTCAA TGTGAGCAAA GGGATAATTC CTGTACTCCA 
TGTGTGCAGA AGACACAGGA TTGTCTAAAT GAAGTGCCAG ATATTTTTCA TTATAATCAG 
CTGCAAATCA TGCCTGCGGA TCTACATCAG TCTGCTGGAT CCCACAGTCC AAATATCGCA 
CTGAAGCTAC TGAATGATGC AGTGATTTCC CAACCCACCT ATATTCCTTT TTTCCAAAGA 
TTAGTATCCA GGCGAAATAC TGAGATCTTA GGAACAATTT CAGCTGGTCC AACTACTGTC 
CTTCATGGAA AAACACCTGC AGACTCAACT CCTCCTGGCT ATAATAAGGA TTATTTAACT 
ACAAACCCTG TTGACCCTTT GCCAAATGCA CACACAACAA TCACCCACAC AGGAGAAATA 
AAGTCTGTTT CAGCATCAGA GCCATTCAGA AACATTATTA TGCCTTCTTC AAAATTGCCT 
CAAAGAGAAG CTACATCATC AAACTCATCT CCACAACCTG AAGAATATTT GCATGAATCT 
TTTCTGGGTT TGCATACACA AAAGTTATCT ACACCTACCT CCTCTTTAGG CATTCCTAAT 
AAGTCTTTCC CTTCAGCAGT TATTTCTACC TCAACACTAA TGCCTCAAAT GTTTCCTTTT 
TCTGATATGT CTTCTGGTCC ATCCAAACTA ACTTCTGACA GCGCTGCCCT TCATCCACCT 
GCTTGGAATG CAGATAGTTC TGTTCAGTCA CCAGCACATG ATTTTATTTC TTCTTTCACC 
CATACACCGG ACTCACATTC TTTATTTACC CACTCACAAA GACCCCTCTC TTCTGGCACT 
TCTGATATAT CATCTATCCA TGCAGATACC ACTCAATTCT CTTTGTTAGC CAAAAACACT 
CTAGGCTATA CCTCGGCCCC TGCACCCCAT GCTGCTCACT TCCCAACCTC TGCAAACATC 
ATCCCGAGTG ATTCTGGGAT TCCTCTCATG CAGCTAAACC ACAGAGAGTC AGAGAGTGAT 
GAACTGGGGA ATGGACACGC AATTGGATGT TCTTTAAAAG GTCTTGAATT TTCCAGTGGC 
TCCATCTTTG TTGCTGACAT GGACTACTGC CTGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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vwce cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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