synj1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following synj1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the synj1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa06437 XM_031896571.1
Latest version!
Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa33904 XM_031896570.1
Latest version!
Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa06435 XM_018091404.2
Latest version!
Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa06435 XM_004912146.4
Latest version!
Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa06436 XM_018091406.2
Latest version!
Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa33901 XM_018091405.2
Latest version!
Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa33902 XM_018091407.2
Latest version!
Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa33903 XM_018091409.2
Latest version!
Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa42637 XM_018091408.1
Latest version!
Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
View Old Accession Versions >>
OXa06436 XM_018091406.1 Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa33903 XM_018091409.1 Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa06435 XM_018091404.1 Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa06435 XM_004912146.3 Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa33901 XM_018091405.1 Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa33902 XM_018091407.1 Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa06437
    Clone ID Related Accession (Same CDS sequence) XM_031896571.1
    Accession Version XM_031896571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGGAGCAACC ATCAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752431.1
    CDS102..4001
    Translation

    Target ORF information:

    RefSeq Version XM_031896571.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGGAGCAACC ATCAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33904
    Clone ID Related Accession (Same CDS sequence) XM_031896570.1
    Accession Version XM_031896570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGCAGGAGCA ACCATCAGGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752430.1
    CDS102..4004
    Translation

    Target ORF information:

    RefSeq Version XM_031896570.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGCAGGAGCA ACCATCAGGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06435
    Clone ID Related Accession (Same CDS sequence) XM_004912146.3 , XM_018091404.1 , XM_018091404.2 , XM_004912146.4
    Accession Version XM_018091404.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091404.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGCTTCCAGC AAACGGAGTC
    GTTGGCTTTA AAAGCCAGTT CGACAATGAT CCTTTTGTGG ACTTTTCATT TGATCAACTT
    CCAAATTCAA AGCTTCAGGC TCCGGTTCCC ATATCCTCCT CAAGCCAAAA GGAGTCATTT
    AAGTTGCCTT CTGCTGTCCA TAATAATCCG AGTGGCATAA CTTGCATGGC TACATTACCT
    CCTGTCCCAG CCCGTACTGG ATTCCAAGAC AGCACACGCA TTTCACCAAA CCCTTTTGTA
    AACAGGGTCA ACACCATCAG CCCAACCAAC CCATTCACAG AAAGATCTTC TAATGCTGGG
    AATCCTTTTA CAGTCCCAGA AATGGCCGCA CATAATCCAC TTCAAACAAG GACTTTAGAT
    CAGTTTAGTT CTACCAGTGA ACCTGAACTT CTGCACAGCA AACCGACCTT TACACTGGGC
    CCTATGGAAA AAAGCCTTTC CCTACCAAAC AAGTCGTACT CGTTTAATGA TGCCAGTCAA
    AAAGGTTGGA TAACGTTTGA CGACGATAAC GGCACCGGCA AAATAAACAG GCATTCTGGC
    AACAGCTCGA CGGTGGATCA TGTAAGTTCA GAAAATGATC TGTTCCAGCT GGGATTTACT
    AATGACTGGA GTGTCACCTC CACTGCTTCC TCCCATACGT TGCCGCTAAG GAAGCCCCCT
    GCCATTCCAT CTCGAACAAA ACGTGAACAG CCATCCACCA ACGTCCCACC AAATAATTCT
    CCAACTCTAG AGTTTACAGA TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946893.1
    CDS100..4746
    Translation

    Target ORF information:

    RefSeq Version XM_018091404.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091404.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGCTTCCAGC AAACGGAGTC
    GTTGGCTTTA AAAGCCAGTT CGACAATGAT CCTTTTGTGG ACTTTTCATT TGATCAACTT
    CCAAATTCAA AGCTTCAGGC TCCGGTTCCC ATATCCTCCT CAAGCCAAAA GGAGTCATTT
    AAGTTGCCTT CTGCTGTCCA TAATAATCCG AGTGGCATAA CTTGCATGGC TACATTACCT
    CCTGTCCCAG CCCGTACTGG ATTCCAAGAC AGCACACGCA TTTCACCAAA CCCTTTTGTA
    AACAGGGTCA ACACCATCAG CCCAACCAAC CCATTCACAG AAAGATCTTC TAATGCTGGG
    AATCCTTTTA CAGTCCCAGA AATGGCCGCA CATAATCCAC TTCAAACAAG GACTTTAGAT
    CAGTTTAGTT CTACCAGTGA ACCTGAACTT CTGCACAGCA AACCGACCTT TACACTGGGC
    CCTATGGAAA AAAGCCTTTC CCTACCAAAC AAGTCGTACT CGTTTAATGA TGCCAGTCAA
    AAAGGTTGGA TAACGTTTGA CGACGATAAC GGCACCGGCA AAATAAACAG GCATTCTGGC
    AACAGCTCGA CGGTGGATCA TGTAAGTTCA GAAAATGATC TGTTCCAGCT GGGATTTACT
    AATGACTGGA GTGTCACCTC CACTGCTTCC TCCCATACGT TGCCGCTAAG GAAGCCCCCT
    GCCATTCCAT CTCGAACAAA ACGTGAACAG CCATCCACCA ACGTCCCACC AAATAATTCT
    CCAACTCTAG AGTTTACAGA TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06435
    Clone ID Related Accession (Same CDS sequence) XM_004912146.3 , XM_018091404.1 , XM_018091404.2 , XM_004912146.4
    Accession Version XM_004912146.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912146.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGCTTCCAGC AAACGGAGTC
    GTTGGCTTTA AAAGCCAGTT CGACAATGAT CCTTTTGTGG ACTTTTCATT TGATCAACTT
    CCAAATTCAA AGCTTCAGGC TCCGGTTCCC ATATCCTCCT CAAGCCAAAA GGAGTCATTT
    AAGTTGCCTT CTGCTGTCCA TAATAATCCG AGTGGCATAA CTTGCATGGC TACATTACCT
    CCTGTCCCAG CCCGTACTGG ATTCCAAGAC AGCACACGCA TTTCACCAAA CCCTTTTGTA
    AACAGGGTCA ACACCATCAG CCCAACCAAC CCATTCACAG AAAGATCTTC TAATGCTGGG
    AATCCTTTTA CAGTCCCAGA AATGGCCGCA CATAATCCAC TTCAAACAAG GACTTTAGAT
    CAGTTTAGTT CTACCAGTGA ACCTGAACTT CTGCACAGCA AACCGACCTT TACACTGGGC
    CCTATGGAAA AAAGCCTTTC CCTACCAAAC AAGTCGTACT CGTTTAATGA TGCCAGTCAA
    AAAGGTTGGA TAACGTTTGA CGACGATAAC GGCACCGGCA AAATAAACAG GCATTCTGGC
    AACAGCTCGA CGGTGGATCA TGTAAGTTCA GAAAATGATC TGTTCCAGCT GGGATTTACT
    AATGACTGGA GTGTCACCTC CACTGCTTCC TCCCATACGT TGCCGCTAAG GAAGCCCCCT
    GCCATTCCAT CTCGAACAAA ACGTGAACAG CCATCCACCA ACGTCCCACC AAATAATTCT
    CCAACTCTAG AGTTTACAGA TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912203.1
    CDS251..4897
    Translation

    Target ORF information:

    RefSeq Version XM_004912146.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912146.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGCTTCCAGC AAACGGAGTC
    GTTGGCTTTA AAAGCCAGTT CGACAATGAT CCTTTTGTGG ACTTTTCATT TGATCAACTT
    CCAAATTCAA AGCTTCAGGC TCCGGTTCCC ATATCCTCCT CAAGCCAAAA GGAGTCATTT
    AAGTTGCCTT CTGCTGTCCA TAATAATCCG AGTGGCATAA CTTGCATGGC TACATTACCT
    CCTGTCCCAG CCCGTACTGG ATTCCAAGAC AGCACACGCA TTTCACCAAA CCCTTTTGTA
    AACAGGGTCA ACACCATCAG CCCAACCAAC CCATTCACAG AAAGATCTTC TAATGCTGGG
    AATCCTTTTA CAGTCCCAGA AATGGCCGCA CATAATCCAC TTCAAACAAG GACTTTAGAT
    CAGTTTAGTT CTACCAGTGA ACCTGAACTT CTGCACAGCA AACCGACCTT TACACTGGGC
    CCTATGGAAA AAAGCCTTTC CCTACCAAAC AAGTCGTACT CGTTTAATGA TGCCAGTCAA
    AAAGGTTGGA TAACGTTTGA CGACGATAAC GGCACCGGCA AAATAAACAG GCATTCTGGC
    AACAGCTCGA CGGTGGATCA TGTAAGTTCA GAAAATGATC TGTTCCAGCT GGGATTTACT
    AATGACTGGA GTGTCACCTC CACTGCTTCC TCCCATACGT TGCCGCTAAG GAAGCCCCCT
    GCCATTCCAT CTCGAACAAA ACGTGAACAG CCATCCACCA ACGTCCCACC AAATAATTCT
    CCAACTCTAG AGTTTACAGA TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06436
    Clone ID Related Accession (Same CDS sequence) XM_018091406.1 , XM_018091406.2
    Accession Version XM_018091406.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091406.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGCATCCCCC AGAGTGCTAC GGTGTATGTG TGAGAACTAC
    TATAGGTATG CAAAGCCGAG GAAAATCCGC ATCTGTGTCG GCACTTGGAA CGTCAACGGT
    GGGAAGCAAT TCCGAAGCAT CGCTTACAAG AATTCAACAC TGACAGACTG GCTGTTAGAT
    GCACCAAGAA TTGCCGGTAT TCAGGAATTT CAAGATAGAC GGAGCAAGCC AGTTGATATA
    TTTGCCATTG GATTTGAAGA AATGGTTGAG TTAAATGCAG GAAACATTGT TAATGCAAGC
    ACAGCCAATC AAAAGCTGTG GGCTACAGAA TTGCAGAAGA CCGTATCAAG AGACCATAAA
    TATGTACTCC TGGCATCAGA ACAGCTTGTA GGTGTGTGCC TCTACATCTT CATCAGACCC
    CAGCATGCAC CATTTATAAG AGATGTTGCT GTTGATACTG TAAAGACCGG CATGGGTGGC
    GCTACAGGAA ACAAAGGAGC TGTTGCCATA CGAATGCTAT TTCATACAAC CAGCCTGTGC
    TTTGTCTGTA GCCACTTTGC AGCGGGGCAG TCTCAAGTCA AAGAGAGAAA TGAGGACTAT
    AATGAAATAG CCCGAAAACT CAGTTTTCCA ATGGGCAGAA TGCTGTTTTC TCACGACTAT
    GTGTTCTGGT GCGGAGACTT TAATTATCGG ATTGATCTGC CAAATGAAGA GGTCAAAGAA
    ATGATCCGAA ATCAAAACTG GGATTCTCTT ATTTTAGGAG ATCAGCTGAT CAACCAGAAG
    AACAGTGGGC AGGTTTTCCG TGGGTTTCAA GAGGGAAAAA TTAATTTTGC TCCTACATAC
    AAGTATGATC TGTTCTCAGA TGACTATGAC ACAAGTGAGA AGTGTCGGAC TCCAGCATGG
    ACAGACCGCG TCCTCTGGAG AAGAAGGAAA TGGCCTTTTG ACAGATCAGC TGAAGATTTA
    GACCTTCTAA ATTCAAGTTT ACAAGAAGGT AAAAATATTC CATATACCTG GAATCCAGGT
    ACATTACTGC ACTACGGCAG AGTTGAGCTG AAAACGTCAG ATCACAGGCC AATTGTGTCT
    CTGATTGATA TAGATATATT TGAAACTGAT GCAGAAGAGC GGCAGAAAAT TTATAAGGAG
    GTGGTTGCAT CCCAGGGCCC TCCAGATGGC ACCATTATGG TCTCTATAGA GAGCAGCTCA
    CCAGAAGGGA ACTATTTTGA TGATAACTTG ATTGACGAGT TGCTCCAGCA GTTTGCAGCT
    TATGGAGAAG TCATACTTAT AAGGTTCGTT GATGATAAAA TGTGGGTCAC GTTTCTGGAA
    GGAGGCTCAG CGCTTGCTGT TCTAAGCTTA AATGGTTCTC AGGTACAGGG AAAAGCAATC
    CATATCCAAA TGAAAAACCC TGATTGGATG GTAGAGTTAG AAGAGCAGAT CAATTTGGAA
    AAGATTAGTC TCAGTTTGCC TTCTTCAACA AGCTCAACCC TGCTCGGTGA AGATTCAGAA
    ATTTCTGCAG ACTATGACAT GGAAGGAGAT GTTGATGATT ACAGTGCAGA GGTAGAAGAA
    ATTCTTCCCC AGCATTTACA GCCATCTTCA TGTTCTACCC TGGGATCATC TCCTAGCTCT
    TCTCCTCGCT CAAGCCCTTG TCCGTCACCA ACAATGCCAG AAGGCCCTCC TCCATCACTC
    CCTGTACGAC CAAGTCGTGC CCCCCAGAAA ACTCCAGGGC CCCCTGTATC TTCATCTCAA
    GCTGAATTTC AACCAGTGGT ACAACAGAAT GCATCATTAA CTAGTGAACC AAAGCGCCCA
    CCGCCTCCCC GCCCTGGTGT GCCCCCTGCA CGCCCAGCAC CACCACAGCG TCCACCTCCT
    CCATCAGGGG CTAGGAGTCC AGCACCAGTT AGAAAGGAGG TTGGAGGTCC AGGGGCTCCT
    CCAAGCCCAG GAGTAGCTCG GAGAGATGTG GAAGTAAGAG GCCAGACTCC TTCACAGATG
    GGACCCCCAG CAATTGCCCC ATGCAGCTCA GTTAGACCGA CCATTCCTCC TCGTGCTGGA
    GTTATCAGTG CTCCACAAAG CCATGTTCGG CCTGTTCCTG CTCGAACAAC CCCTGAGGTC
    CCAAACAAGC CCACCGAACC TGCAAGAGGT GTTCCGTTAC TTCCTGAGCC CTTGAAGCCT
    CAACCTGCGG CTCCTCTTCA AACCAGTCCA AATTCCCAAC CACCCTGCCA AAAACTGCCT
    GAACCTATTC TACCTACAGC AGTACAGGCC GCATCCCAGA CTCCACCCTT ACAGCAAAAT
    CTAGACCCTC CTCCACTCCC TCCTCCAAGA AGCCGCTCCT CCCAGAACCT GCCCACTGAC
    CAAAATGCTT CTCAGCAGCT TCCAGCAAAC GGAGTCGTTG GCTTTAAAAG CCAGTTCGAC
    AATGATCCTT TTGTGGACTT TTCATTTGAT CAACTTCCAA ATTCAAAGCT TCAGGCTCCG
    GTTCCCATAT CCTCCTCAAG CCAAAAGGAG TCATTTAAGT TGCCTTCTGC TGTCCATAAT
    AATCCGAGTG GCATAACTTG CATGGCTACA TTACCTCCTG TCCCAGCCCG TACTGGATTC
    CAAGACAGCA CACGCATTTC ACCAAACCCT TTTGTAAACA GGGTCAACAC CATCAGCCCA
    ACCAACCCAT TCACAGAAAG ATCTTCTAAT GCTGGGAATC CTTTTACAGT CCCAGAAATG
    GCCGCACATA ATCCACTTCA AACAAGGACT TTAGATCAGT TTAGTTCTAC CAGTGAACCT
    GAACTTCTGC ACAGCAAACC GACCTTTACA CTGGGCCCTA TGGAAAAAAG CCTTTCCCTA
    CCAAACAAGT CGTACTCGTT TAATGATGCC AGTCAAAAAG GTTGGATAAC GTTTGACGAC
    GATAACGGCA CCGGCAAAAT AAACAGGCAT TCTGGCAACA GCTCGACGGT GGATCATGTA
    AGTTCAGAAA ATGATCTGTT CCAGCTGGGA TTTACTAATG ACTGGAGTGT CACCTCCACT
    GCTTCCTCCC ATACGTTGCC GCTAAGGAAG CCCCCTGCCA TTCCATCTCG AACAAAACGT
    GAACAGCCAT CCACCAACGT CCCACCAAAT AATTCTCCAA CTCTAGAGTT TACAGATCGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946895.1
    CDS99..4721
    Translation

    Target ORF information:

    RefSeq Version XM_018091406.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091406.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGCATCCCCC AGAGTGCTAC GGTGTATGTG TGAGAACTAC
    TATAGGTATG CAAAGCCGAG GAAAATCCGC ATCTGTGTCG GCACTTGGAA CGTCAACGGT
    GGGAAGCAAT TCCGAAGCAT CGCTTACAAG AATTCAACAC TGACAGACTG GCTGTTAGAT
    GCACCAAGAA TTGCCGGTAT TCAGGAATTT CAAGATAGAC GGAGCAAGCC AGTTGATATA
    TTTGCCATTG GATTTGAAGA AATGGTTGAG TTAAATGCAG GAAACATTGT TAATGCAAGC
    ACAGCCAATC AAAAGCTGTG GGCTACAGAA TTGCAGAAGA CCGTATCAAG AGACCATAAA
    TATGTACTCC TGGCATCAGA ACAGCTTGTA GGTGTGTGCC TCTACATCTT CATCAGACCC
    CAGCATGCAC CATTTATAAG AGATGTTGCT GTTGATACTG TAAAGACCGG CATGGGTGGC
    GCTACAGGAA ACAAAGGAGC TGTTGCCATA CGAATGCTAT TTCATACAAC CAGCCTGTGC
    TTTGTCTGTA GCCACTTTGC AGCGGGGCAG TCTCAAGTCA AAGAGAGAAA TGAGGACTAT
    AATGAAATAG CCCGAAAACT CAGTTTTCCA ATGGGCAGAA TGCTGTTTTC TCACGACTAT
    GTGTTCTGGT GCGGAGACTT TAATTATCGG ATTGATCTGC CAAATGAAGA GGTCAAAGAA
    ATGATCCGAA ATCAAAACTG GGATTCTCTT ATTTTAGGAG ATCAGCTGAT CAACCAGAAG
    AACAGTGGGC AGGTTTTCCG TGGGTTTCAA GAGGGAAAAA TTAATTTTGC TCCTACATAC
    AAGTATGATC TGTTCTCAGA TGACTATGAC ACAAGTGAGA AGTGTCGGAC TCCAGCATGG
    ACAGACCGCG TCCTCTGGAG AAGAAGGAAA TGGCCTTTTG ACAGATCAGC TGAAGATTTA
    GACCTTCTAA ATTCAAGTTT ACAAGAAGGT AAAAATATTC CATATACCTG GAATCCAGGT
    ACATTACTGC ACTACGGCAG AGTTGAGCTG AAAACGTCAG ATCACAGGCC AATTGTGTCT
    CTGATTGATA TAGATATATT TGAAACTGAT GCAGAAGAGC GGCAGAAAAT TTATAAGGAG
    GTGGTTGCAT CCCAGGGCCC TCCAGATGGC ACCATTATGG TCTCTATAGA GAGCAGCTCA
    CCAGAAGGGA ACTATTTTGA TGATAACTTG ATTGACGAGT TGCTCCAGCA GTTTGCAGCT
    TATGGAGAAG TCATACTTAT AAGGTTCGTT GATGATAAAA TGTGGGTCAC GTTTCTGGAA
    GGAGGCTCAG CGCTTGCTGT TCTAAGCTTA AATGGTTCTC AGGTACAGGG AAAAGCAATC
    CATATCCAAA TGAAAAACCC TGATTGGATG GTAGAGTTAG AAGAGCAGAT CAATTTGGAA
    AAGATTAGTC TCAGTTTGCC TTCTTCAACA AGCTCAACCC TGCTCGGTGA AGATTCAGAA
    ATTTCTGCAG ACTATGACAT GGAAGGAGAT GTTGATGATT ACAGTGCAGA GGTAGAAGAA
    ATTCTTCCCC AGCATTTACA GCCATCTTCA TGTTCTACCC TGGGATCATC TCCTAGCTCT
    TCTCCTCGCT CAAGCCCTTG TCCGTCACCA ACAATGCCAG AAGGCCCTCC TCCATCACTC
    CCTGTACGAC CAAGTCGTGC CCCCCAGAAA ACTCCAGGGC CCCCTGTATC TTCATCTCAA
    GCTGAATTTC AACCAGTGGT ACAACAGAAT GCATCATTAA CTAGTGAACC AAAGCGCCCA
    CCGCCTCCCC GCCCTGGTGT GCCCCCTGCA CGCCCAGCAC CACCACAGCG TCCACCTCCT
    CCATCAGGGG CTAGGAGTCC AGCACCAGTT AGAAAGGAGG TTGGAGGTCC AGGGGCTCCT
    CCAAGCCCAG GAGTAGCTCG GAGAGATGTG GAAGTAAGAG GCCAGACTCC TTCACAGATG
    GGACCCCCAG CAATTGCCCC ATGCAGCTCA GTTAGACCGA CCATTCCTCC TCGTGCTGGA
    GTTATCAGTG CTCCACAAAG CCATGTTCGG CCTGTTCCTG CTCGAACAAC CCCTGAGGTC
    CCAAACAAGC CCACCGAACC TGCAAGAGGT GTTCCGTTAC TTCCTGAGCC CTTGAAGCCT
    CAACCTGCGG CTCCTCTTCA AACCAGTCCA AATTCCCAAC CACCCTGCCA AAAACTGCCT
    GAACCTATTC TACCTACAGC AGTACAGGCC GCATCCCAGA CTCCACCCTT ACAGCAAAAT
    CTAGACCCTC CTCCACTCCC TCCTCCAAGA AGCCGCTCCT CCCAGAACCT GCCCACTGAC
    CAAAATGCTT CTCAGCAGCT TCCAGCAAAC GGAGTCGTTG GCTTTAAAAG CCAGTTCGAC
    AATGATCCTT TTGTGGACTT TTCATTTGAT CAACTTCCAA ATTCAAAGCT TCAGGCTCCG
    GTTCCCATAT CCTCCTCAAG CCAAAAGGAG TCATTTAAGT TGCCTTCTGC TGTCCATAAT
    AATCCGAGTG GCATAACTTG CATGGCTACA TTACCTCCTG TCCCAGCCCG TACTGGATTC
    CAAGACAGCA CACGCATTTC ACCAAACCCT TTTGTAAACA GGGTCAACAC CATCAGCCCA
    ACCAACCCAT TCACAGAAAG ATCTTCTAAT GCTGGGAATC CTTTTACAGT CCCAGAAATG
    GCCGCACATA ATCCACTTCA AACAAGGACT TTAGATCAGT TTAGTTCTAC CAGTGAACCT
    GAACTTCTGC ACAGCAAACC GACCTTTACA CTGGGCCCTA TGGAAAAAAG CCTTTCCCTA
    CCAAACAAGT CGTACTCGTT TAATGATGCC AGTCAAAAAG GTTGGATAAC GTTTGACGAC
    GATAACGGCA CCGGCAAAAT AAACAGGCAT TCTGGCAACA GCTCGACGGT GGATCATGTA
    AGTTCAGAAA ATGATCTGTT CCAGCTGGGA TTTACTAATG ACTGGAGTGT CACCTCCACT
    GCTTCCTCCC ATACGTTGCC GCTAAGGAAG CCCCCTGCCA TTCCATCTCG AACAAAACGT
    GAACAGCCAT CCACCAACGT CCCACCAAAT AATTCTCCAA CTCTAGAGTT TACAGATCGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33901
    Clone ID Related Accession (Same CDS sequence) XM_018091405.2 , XM_018091405.1
    Accession Version XM_018091405.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091405.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGCTGA AAGATGGAGC ACGATCTGTG
    ACCAGAACCA TTCAGAATAA TTTTTTTGAT GGCTCCAAAC AAGAAGCGAT AGATGTTTTG
    CTTCTGGGAA ATACCCTAAA TAGTGATCTA GCCGACAAAG CAAGGGCGCT TCTGACCACT
    GGAAGTTTAC ATGTGTCAGA ACTGATGCTG CAGTCAGCAT CCCCCAGAGT GCTACGGTGT
    ATGTGTGAGA ACTACTATAG GTATGCAAAG CCGAGGAAAA TCCGCATCTG TGTCGGCACT
    TGGAACGTCA ACGGTGGGAA GCAATTCCGA AGCATCGCTT ACAAGAATTC AACACTGACA
    GACTGGCTGT TAGATGCACC AAGAATTGCC GGTATTCAGG AATTTCAAGA TAGACGGAGC
    AAGCCAGTTG ATATATTTGC CATTGGATTT GAAGAAATGG TTGAGTTAAA TGCAGGAAAC
    ATTGTTAATG CAAGCACAGC CAATCAAAAG CTGTGGGCTA CAGAATTGCA GAAGACCGTA
    TCAAGAGACC ATAAATATGT ACTCCTGGCA TCAGAACAGC TTGTAGGTGT GTGCCTCTAC
    ATCTTCATCA GACCCCAGCA TGCACCATTT ATAAGAGATG TTGCTGTTGA TACTGTAAAG
    ACCGGCATGG GTGGCGCTAC AGGAAACAAA GGAGCTGTTG CCATACGAAT GCTATTTCAT
    ACAACCAGCC TGTGCTTTGT CTGTAGCCAC TTTGCAGCGG GGCAGTCTCA AGTCAAAGAG
    AGAAATGAGG ACTATAATGA AATAGCCCGA AAACTCAGTT TTCCAATGGG CAGAATGCTG
    TTTTCTCACG ACTATGTGTT CTGGTGCGGA GACTTTAATT ATCGGATTGA TCTGCCAAAT
    GAAGAGGTCA AAGAAATGAT CCGAAATCAA AACTGGGATT CTCTTATTTT AGGAGATCAG
    CTGATCAACC AGAAGAACAG TGGGCAGGTT TTCCGTGGGT TTCAAGAGGG AAAAATTAAT
    TTTGCTCCTA CATACAAGTA TGATCTGTTC TCAGATGACT ATGACACAAG TGAGAAGTGT
    CGGACTCCAG CATGGACAGA CCGCGTCCTC TGGAGAAGAA GGAAATGGCC TTTTGACAGA
    TCAGCTGAAG ATTTAGACCT TCTAAATTCA AGTTTACAAG AAGGTAAAAA TATTCCATAT
    ACCTGGAATC CAGGTACATT ACTGCACTAC GGCAGAGTTG AGCTGAAAAC GTCAGATCAC
    AGGCCAATTG TGTCTCTGAT TGATATAGAT ATATTTGAAA CTGATGCAGA AGAGCGGCAG
    AAAATTTATA AGGAGGTGGT TGCATCCCAG GGCCCTCCAG ATGGCACCAT TATGGTCTCT
    ATAGAGAGCA GCTCACCAGA AGGGAACTAT TTTGATGATA ACTTGATTGA CGAGTTGCTC
    CAGCAGTTTG CAGCTTATGG AGAAGTCATA CTTATAAGGT TCGTTGATGA TAAAATGTGG
    GTCACGTTTC TGGAAGGAGG CTCAGCGCTT GCTGTTCTAA GCTTAAATGG TTCTCAGGTA
    CAGGGAAAAG CAATCCATAT CCAAATGAAA AACCCTGATT GGATGGTAGA GTTAGAAGAG
    CAGATCAATT TGGAAAAGAT TAGTCTCAGT TTGCCTTCTT CAACAAGCTC AACCCTGCTC
    GGTGAAGATT CAGAAATTTC TGCAGACTAT GACATGGAAG GAGATGTTGA TGATTACAGT
    GCAGAGGTAG AAGAAATTCT TCCCCAGCAT TTACAGCCAT CTTCATGTTC TACCCTGGGA
    TCATCTCCTA GCTCTTCTCC TCGCTCAAGC CCTTGTCCGT CACCAACAAT GCCAGAAGGC
    CCTCCTCCAT CACTCCCTGT ACGACCAAGT CGTGCCCCCC AGAAAACTCC AGGGCCCCCT
    GTATCTTCAT CTCAAGCTGA ATTTCAACCA GTGGTACAAC AGAATGCATC ATTAACTAGT
    GAACCAAAGC GCCCACCGCC TCCCCGCCCT GGTGTGCCCC CTGCACGCCC AGCACCACCA
    CAGCGTCCAC CTCCTCCATC AGGGGCTAGG AGTCCAGCAC CAGTTAGAAA GGAGGTTGGA
    GGTCCAGGGG CTCCTCCAAG CCCAGGAGTA GCTCGGAGAG ATGTGGAAGT AAGAGGCCAG
    ACTCCTTCAC AGATGGGACC CCCAGCAATT GCCCCATGCA GCTCAGTTAG ACCGACCATT
    CCTCCTCGTG CTGGAGTTAT CAGTGCTCCA CAAAGCCATG TTCGGCCTGT TCCTGCTCGA
    ACAACCCCTG AGGTCCCAAA CAAGCCCACC GAACCTGCAA GAGGTGTTCC GTTACTTCCT
    GAGCCCTTGA AGCCTCAACC TGCGGCTCCT CTTCAAACCA GTCCAAATTC CCAACCACCC
    TGCCAAAAAC TGCCTGAACC TATTCTACCT ACAGCAGTAC AGGCCGCATC CCAGACTCCA
    CCCTTACAGC AAAATCTAGA CCCTCCTCCA CTCCCTCCTC CAAGAAGCCG CTCCTCCCAG
    AACCTGCCCA CTGACCAAAA TGCTTCTCAG CAGCTTCCAG CAAACGGAGT CGTTGGCTTT
    AAAAGCCAGT TCGACAATGA TCCTTTTGTG GACTTTTCAT TTGATCAACT TCCAAATTCA
    AAGCTTCAGG CTCCGGTTCC CATATCCTCC TCAAGCCAAA AGGAGTCATT TAAGTTGCCT
    TCTGCTGTCC ATAATAATCC GAGTGGCATA ACTTGCATGG CTACATTACC TCCTGTCCCA
    GCCCGTACTG GATTCCAAGA CAGCACACGC ATTTCACCAA ACCCTTTTGT AAACAGGGTC
    AACACCATCA GCCCAACCAA CCCATTCACA GAAAGATCTT CTAATGCTGG GAATCCTTTT
    ACAGTCCCAG AAATGGCCGC ACATAATCCA CTTCAAACAA GGACTTTAGA TCAGTTTAGT
    TCTACCAGTG AACCTGAACT TCTGCACAGC AAACCGACCT TTACACTGGG CCCTATGGAA
    AAAAGCCTTT CCCTACCAAA CAAGTCGTAC TCGTTTAATG ATGCCAGTCA AAAAGGTTGG
    ATAACGTTTG ACGACGATAA CGGCACCGGC AAAATAAACA GGCATTCTGG CAACAGCTCG
    ACGGTGGATC ATGTAAGTTC AGAAAATGAT CTGTTCCAGC TGGGATTTAC TAATGACTGG
    AGTGTCACCT CCACTGCTTC CTCCCATACG TTGCCGCTAA GGAAGCCCCC TGCCATTCCA
    TCTCGAACAA AACGTGAACA GCCATCCACC AACGTCCCAC CAAATAATTC TCCAACTCTA
    GAGTTTACAG ATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946894.1
    CDS100..4737
    Translation

    Target ORF information:

    RefSeq Version XM_018091405.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091405.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGCTGA AAGATGGAGC ACGATCTGTG
    ACCAGAACCA TTCAGAATAA TTTTTTTGAT GGCTCCAAAC AAGAAGCGAT AGATGTTTTG
    CTTCTGGGAA ATACCCTAAA TAGTGATCTA GCCGACAAAG CAAGGGCGCT TCTGACCACT
    GGAAGTTTAC ATGTGTCAGA ACTGATGCTG CAGTCAGCAT CCCCCAGAGT GCTACGGTGT
    ATGTGTGAGA ACTACTATAG GTATGCAAAG CCGAGGAAAA TCCGCATCTG TGTCGGCACT
    TGGAACGTCA ACGGTGGGAA GCAATTCCGA AGCATCGCTT ACAAGAATTC AACACTGACA
    GACTGGCTGT TAGATGCACC AAGAATTGCC GGTATTCAGG AATTTCAAGA TAGACGGAGC
    AAGCCAGTTG ATATATTTGC CATTGGATTT GAAGAAATGG TTGAGTTAAA TGCAGGAAAC
    ATTGTTAATG CAAGCACAGC CAATCAAAAG CTGTGGGCTA CAGAATTGCA GAAGACCGTA
    TCAAGAGACC ATAAATATGT ACTCCTGGCA TCAGAACAGC TTGTAGGTGT GTGCCTCTAC
    ATCTTCATCA GACCCCAGCA TGCACCATTT ATAAGAGATG TTGCTGTTGA TACTGTAAAG
    ACCGGCATGG GTGGCGCTAC AGGAAACAAA GGAGCTGTTG CCATACGAAT GCTATTTCAT
    ACAACCAGCC TGTGCTTTGT CTGTAGCCAC TTTGCAGCGG GGCAGTCTCA AGTCAAAGAG
    AGAAATGAGG ACTATAATGA AATAGCCCGA AAACTCAGTT TTCCAATGGG CAGAATGCTG
    TTTTCTCACG ACTATGTGTT CTGGTGCGGA GACTTTAATT ATCGGATTGA TCTGCCAAAT
    GAAGAGGTCA AAGAAATGAT CCGAAATCAA AACTGGGATT CTCTTATTTT AGGAGATCAG
    CTGATCAACC AGAAGAACAG TGGGCAGGTT TTCCGTGGGT TTCAAGAGGG AAAAATTAAT
    TTTGCTCCTA CATACAAGTA TGATCTGTTC TCAGATGACT ATGACACAAG TGAGAAGTGT
    CGGACTCCAG CATGGACAGA CCGCGTCCTC TGGAGAAGAA GGAAATGGCC TTTTGACAGA
    TCAGCTGAAG ATTTAGACCT TCTAAATTCA AGTTTACAAG AAGGTAAAAA TATTCCATAT
    ACCTGGAATC CAGGTACATT ACTGCACTAC GGCAGAGTTG AGCTGAAAAC GTCAGATCAC
    AGGCCAATTG TGTCTCTGAT TGATATAGAT ATATTTGAAA CTGATGCAGA AGAGCGGCAG
    AAAATTTATA AGGAGGTGGT TGCATCCCAG GGCCCTCCAG ATGGCACCAT TATGGTCTCT
    ATAGAGAGCA GCTCACCAGA AGGGAACTAT TTTGATGATA ACTTGATTGA CGAGTTGCTC
    CAGCAGTTTG CAGCTTATGG AGAAGTCATA CTTATAAGGT TCGTTGATGA TAAAATGTGG
    GTCACGTTTC TGGAAGGAGG CTCAGCGCTT GCTGTTCTAA GCTTAAATGG TTCTCAGGTA
    CAGGGAAAAG CAATCCATAT CCAAATGAAA AACCCTGATT GGATGGTAGA GTTAGAAGAG
    CAGATCAATT TGGAAAAGAT TAGTCTCAGT TTGCCTTCTT CAACAAGCTC AACCCTGCTC
    GGTGAAGATT CAGAAATTTC TGCAGACTAT GACATGGAAG GAGATGTTGA TGATTACAGT
    GCAGAGGTAG AAGAAATTCT TCCCCAGCAT TTACAGCCAT CTTCATGTTC TACCCTGGGA
    TCATCTCCTA GCTCTTCTCC TCGCTCAAGC CCTTGTCCGT CACCAACAAT GCCAGAAGGC
    CCTCCTCCAT CACTCCCTGT ACGACCAAGT CGTGCCCCCC AGAAAACTCC AGGGCCCCCT
    GTATCTTCAT CTCAAGCTGA ATTTCAACCA GTGGTACAAC AGAATGCATC ATTAACTAGT
    GAACCAAAGC GCCCACCGCC TCCCCGCCCT GGTGTGCCCC CTGCACGCCC AGCACCACCA
    CAGCGTCCAC CTCCTCCATC AGGGGCTAGG AGTCCAGCAC CAGTTAGAAA GGAGGTTGGA
    GGTCCAGGGG CTCCTCCAAG CCCAGGAGTA GCTCGGAGAG ATGTGGAAGT AAGAGGCCAG
    ACTCCTTCAC AGATGGGACC CCCAGCAATT GCCCCATGCA GCTCAGTTAG ACCGACCATT
    CCTCCTCGTG CTGGAGTTAT CAGTGCTCCA CAAAGCCATG TTCGGCCTGT TCCTGCTCGA
    ACAACCCCTG AGGTCCCAAA CAAGCCCACC GAACCTGCAA GAGGTGTTCC GTTACTTCCT
    GAGCCCTTGA AGCCTCAACC TGCGGCTCCT CTTCAAACCA GTCCAAATTC CCAACCACCC
    TGCCAAAAAC TGCCTGAACC TATTCTACCT ACAGCAGTAC AGGCCGCATC CCAGACTCCA
    CCCTTACAGC AAAATCTAGA CCCTCCTCCA CTCCCTCCTC CAAGAAGCCG CTCCTCCCAG
    AACCTGCCCA CTGACCAAAA TGCTTCTCAG CAGCTTCCAG CAAACGGAGT CGTTGGCTTT
    AAAAGCCAGT TCGACAATGA TCCTTTTGTG GACTTTTCAT TTGATCAACT TCCAAATTCA
    AAGCTTCAGG CTCCGGTTCC CATATCCTCC TCAAGCCAAA AGGAGTCATT TAAGTTGCCT
    TCTGCTGTCC ATAATAATCC GAGTGGCATA ACTTGCATGG CTACATTACC TCCTGTCCCA
    GCCCGTACTG GATTCCAAGA CAGCACACGC ATTTCACCAA ACCCTTTTGT AAACAGGGTC
    AACACCATCA GCCCAACCAA CCCATTCACA GAAAGATCTT CTAATGCTGG GAATCCTTTT
    ACAGTCCCAG AAATGGCCGC ACATAATCCA CTTCAAACAA GGACTTTAGA TCAGTTTAGT
    TCTACCAGTG AACCTGAACT TCTGCACAGC AAACCGACCT TTACACTGGG CCCTATGGAA
    AAAAGCCTTT CCCTACCAAA CAAGTCGTAC TCGTTTAATG ATGCCAGTCA AAAAGGTTGG
    ATAACGTTTG ACGACGATAA CGGCACCGGC AAAATAAACA GGCATTCTGG CAACAGCTCG
    ACGGTGGATC ATGTAAGTTC AGAAAATGAT CTGTTCCAGC TGGGATTTAC TAATGACTGG
    AGTGTCACCT CCACTGCTTC CTCCCATACG TTGCCGCTAA GGAAGCCCCC TGCCATTCCA
    TCTCGAACAA AACGTGAACA GCCATCCACC AACGTCCCAC CAAATAATTC TCCAACTCTA
    GAGTTTACAG ATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33902
    Clone ID Related Accession (Same CDS sequence) XM_018091407.2 , XM_018091407.1
    Accession Version XM_018091407.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4608bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091407.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGTCCAGGGG CTCCTCCAAG CCCAGGAGTA
    GCTCGGAGAG ATGTGGAAGT AAGAGGCCAG ACTCCTTCAC AGATGGGACC CCCAGCAATT
    GCCCCATGCA GCTCAGTTAG ACCGACCATT CCTCCTCGTG CTGGAGTTAT CAGTGCTCCA
    CAAAGCCATG TTCGGCCTGT TCCTGCTCGA ACAACCCCTG AGGTCCCAAA CAAGCCCACC
    GAACCTGCAA GAGGTGTTCC GTTACTTCCT GAGCCCTTGA AGCCTCAACC TGCGGCTCCT
    CTTCAAACCA GTCCAAATTC CCAACCACCC TGCCAAAAAC TGCCTGAACC TATTCTACCT
    ACAGCAGTAC AGGCCGCATC CCAGACTCCA CCCTTACAGC AAAATCTAGA CCCTCCTCCA
    CTCCCTCCTC CAAGAAGCCG CTCCTCCCAG AACCTGCCCA CTGACCAAAA TGCTTCTCAG
    CAGCTTCCAG CAAACGGAGT CGTTGGCTTT AAAAGCCAGT TCGACAATGA TCCTTTTGTG
    GACTTTTCAT TTGATCAACT TCCAAATTCA AAGCTTCAGG CTCCGGTTCC CATATCCTCC
    TCAAGCCAAA AGGAGTCATT TAAGTTGCCT TCTGCTGTCC ATAATAATCC GAGTGGCATA
    ACTTGCATGG CTACATTACC TCCTGTCCCA GCCCGTACTG GATTCCAAGA CAGCACACGC
    ATTTCACCAA ACCCTTTTGT AAACAGGGTC AACACCATCA GCCCAACCAA CCCATTCACA
    GAAAGATCTT CTAATGCTGG GAATCCTTTT ACAGTCCCAG AAATGGCCGC ACATAATCCA
    CTTCAAACAA GGACTTTAGA TCAGTTTAGT TCTACCAGTG AACCTGAACT TCTGCACAGC
    AAACCGACCT TTACACTGGG CCCTATGGAA AAAAGCCTTT CCCTACCAAA CAAGTCGTAC
    TCGTTTAATG ATGCCAGTCA AAAAGGTTGG ATAACGTTTG ACGACGATAA CGGCACCGGC
    AAAATAAACA GGCATTCTGG CAACAGCTCG ACGGTGGATC ATGTAAGTTC AGAAAATGAT
    CTGTTCCAGC TGGGATTTAC TAATGACTGG AGTGTCACCT CCACTGCTTC CTCCCATACG
    TTGCCGCTAA GGAAGCCCCC TGCCATTCCA TCTCGAACAA AACGTGAACA GCCATCCACC
    AACGTCCCAC CAAATAATTC TCCAACTCTA GAGTTTACAG ATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946896.1
    CDS100..4707
    Translation

    Target ORF information:

    RefSeq Version XM_018091407.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091407.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGTCCAGGGG CTCCTCCAAG CCCAGGAGTA
    GCTCGGAGAG ATGTGGAAGT AAGAGGCCAG ACTCCTTCAC AGATGGGACC CCCAGCAATT
    GCCCCATGCA GCTCAGTTAG ACCGACCATT CCTCCTCGTG CTGGAGTTAT CAGTGCTCCA
    CAAAGCCATG TTCGGCCTGT TCCTGCTCGA ACAACCCCTG AGGTCCCAAA CAAGCCCACC
    GAACCTGCAA GAGGTGTTCC GTTACTTCCT GAGCCCTTGA AGCCTCAACC TGCGGCTCCT
    CTTCAAACCA GTCCAAATTC CCAACCACCC TGCCAAAAAC TGCCTGAACC TATTCTACCT
    ACAGCAGTAC AGGCCGCATC CCAGACTCCA CCCTTACAGC AAAATCTAGA CCCTCCTCCA
    CTCCCTCCTC CAAGAAGCCG CTCCTCCCAG AACCTGCCCA CTGACCAAAA TGCTTCTCAG
    CAGCTTCCAG CAAACGGAGT CGTTGGCTTT AAAAGCCAGT TCGACAATGA TCCTTTTGTG
    GACTTTTCAT TTGATCAACT TCCAAATTCA AAGCTTCAGG CTCCGGTTCC CATATCCTCC
    TCAAGCCAAA AGGAGTCATT TAAGTTGCCT TCTGCTGTCC ATAATAATCC GAGTGGCATA
    ACTTGCATGG CTACATTACC TCCTGTCCCA GCCCGTACTG GATTCCAAGA CAGCACACGC
    ATTTCACCAA ACCCTTTTGT AAACAGGGTC AACACCATCA GCCCAACCAA CCCATTCACA
    GAAAGATCTT CTAATGCTGG GAATCCTTTT ACAGTCCCAG AAATGGCCGC ACATAATCCA
    CTTCAAACAA GGACTTTAGA TCAGTTTAGT TCTACCAGTG AACCTGAACT TCTGCACAGC
    AAACCGACCT TTACACTGGG CCCTATGGAA AAAAGCCTTT CCCTACCAAA CAAGTCGTAC
    TCGTTTAATG ATGCCAGTCA AAAAGGTTGG ATAACGTTTG ACGACGATAA CGGCACCGGC
    AAAATAAACA GGCATTCTGG CAACAGCTCG ACGGTGGATC ATGTAAGTTC AGAAAATGAT
    CTGTTCCAGC TGGGATTTAC TAATGACTGG AGTGTCACCT CCACTGCTTC CTCCCATACG
    TTGCCGCTAA GGAAGCCCCC TGCCATTCCA TCTCGAACAA AACGTGAACA GCCATCCACC
    AACGTCCCAC CAAATAATTC TCCAACTCTA GAGTTTACAG ATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33903
    Clone ID Related Accession (Same CDS sequence) XM_018091409.1 , XM_018091409.2
    Accession Version XM_018091409.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GTAAGAGGCC AGACTCCTTC ACAGATGGGA
    CCCCCAGCAA TTGCCCCATG CAGCTCAGTT AGACCGACCA TTCCTCCTCG TGCTGGAGTT
    ATCAGTGCTC CACAAAGCCA TGTTCGGCCT GTTCCTGCTC GAACAACCCC TGAGGTCCCA
    AACAAGCCCA CCGAACCTGC AAGAGGTGTT CCGTTACTTC CTGAGCCCTT GAAGCCTCAA
    CCTGCGGCTC CTCTTCAAAC CAGTCCAAAT TCCCAACCAC CCTGCCAAAA ACTGCCTGAA
    CCTATTCTAC CTACAGCAGT ACAGGCCGCA TCCCAGACTC CACCCTTACA GCAAAATCTA
    GACCCTCCTC CACTCCCTCC TCCAAGAAGC CGCTCCTCCC AGAACCTGCC CACTGACCAA
    AATGCTTCTC AGCAGCTTCC AGCAAACGGA GTCGTTGGCT TTAAAAGCCA GTTCGACAAT
    GATCCTTTTG TGGACTTTTC ATTTGATCAA CTTCCAAATT CAAAGCTTCA GGCTCCGGTT
    CCCATATCCT CCTCAAGCCA AAAGGAGTCA TTTAAGTTGC CTTCTGCTGT CCATAATAAT
    CCGAGTGGCA TAACTTGCAT GGCTACATTA CCTCCTGTCC CAGCCCGTAC TGGATTCCAA
    GACAGCACAC GCATTTCACC AAACCCTTTT GTAAACAGGG TCAACACCAT CAGCCCAACC
    AACCCATTCA CAGAAAGATC TTCTAATGCT GGGAATCCTT TTACAGTCCC AGAAATGGCC
    GCACATAATC CACTTCAAAC AAGGACTTTA GATCAGTTTA GTTCTACCAG TGAACCTGAA
    CTTCTGCACA GCAAACCGAC CTTTACACTG GGCCCTATGG AAAAAAGCCT TTCCCTACCA
    AACAAGTCGT ACTCGTTTAA TGATGCCAGT CAAAAAGGTT GGATAACGTT TGACGACGAT
    AACGGCACCG GCAAAATAAA CAGGCATTCT GGCAACAGCT CGACGGTGGA TCATGTAAGT
    TCAGAAAATG ATCTGTTCCA GCTGGGATTT ACTAATGACT GGAGTGTCAC CTCCACTGCT
    TCCTCCCATA CGTTGCCGCT AAGGAAGCCC CCTGCCATTC CATCTCGAAC AAAACGTGAA
    CAGCCATCCA CCAACGTCCC ACCAAATAAT TCTCCAACTC TAGAGTTTAC AGATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946898.1
    CDS99..4658
    Translation

    Target ORF information:

    RefSeq Version XM_018091409.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091409.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GTAAGAGGCC AGACTCCTTC ACAGATGGGA
    CCCCCAGCAA TTGCCCCATG CAGCTCAGTT AGACCGACCA TTCCTCCTCG TGCTGGAGTT
    ATCAGTGCTC CACAAAGCCA TGTTCGGCCT GTTCCTGCTC GAACAACCCC TGAGGTCCCA
    AACAAGCCCA CCGAACCTGC AAGAGGTGTT CCGTTACTTC CTGAGCCCTT GAAGCCTCAA
    CCTGCGGCTC CTCTTCAAAC CAGTCCAAAT TCCCAACCAC CCTGCCAAAA ACTGCCTGAA
    CCTATTCTAC CTACAGCAGT ACAGGCCGCA TCCCAGACTC CACCCTTACA GCAAAATCTA
    GACCCTCCTC CACTCCCTCC TCCAAGAAGC CGCTCCTCCC AGAACCTGCC CACTGACCAA
    AATGCTTCTC AGCAGCTTCC AGCAAACGGA GTCGTTGGCT TTAAAAGCCA GTTCGACAAT
    GATCCTTTTG TGGACTTTTC ATTTGATCAA CTTCCAAATT CAAAGCTTCA GGCTCCGGTT
    CCCATATCCT CCTCAAGCCA AAAGGAGTCA TTTAAGTTGC CTTCTGCTGT CCATAATAAT
    CCGAGTGGCA TAACTTGCAT GGCTACATTA CCTCCTGTCC CAGCCCGTAC TGGATTCCAA
    GACAGCACAC GCATTTCACC AAACCCTTTT GTAAACAGGG TCAACACCAT CAGCCCAACC
    AACCCATTCA CAGAAAGATC TTCTAATGCT GGGAATCCTT TTACAGTCCC AGAAATGGCC
    GCACATAATC CACTTCAAAC AAGGACTTTA GATCAGTTTA GTTCTACCAG TGAACCTGAA
    CTTCTGCACA GCAAACCGAC CTTTACACTG GGCCCTATGG AAAAAAGCCT TTCCCTACCA
    AACAAGTCGT ACTCGTTTAA TGATGCCAGT CAAAAAGGTT GGATAACGTT TGACGACGAT
    AACGGCACCG GCAAAATAAA CAGGCATTCT GGCAACAGCT CGACGGTGGA TCATGTAAGT
    TCAGAAAATG ATCTGTTCCA GCTGGGATTT ACTAATGACT GGAGTGTCAC CTCCACTGCT
    TCCTCCCATA CGTTGCCGCT AAGGAAGCCC CCTGCCATTC CATCTCGAAC AAAACGTGAA
    CAGCCATCCA CCAACGTCCC ACCAAATAAT TCTCCAACTC TAGAGTTTAC AGATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42637
    Clone ID Related Accession (Same CDS sequence) XM_018091408.1
    Accession Version XM_018091408.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG TAAGAGGCCA GACTCCTTCA CAGATGGGAC CCCCAGCAAT TGCCCCATGC
    AGCTCAGTTA GACCGACCAT TCCTCCTCGT GCTGGAGTTA TCAGTGCTCC ACAAAGCCAT
    GTTCGGCCTG TTCCTGCTCG AACAACCCCT GAGGTCCCAA ACAAGCCCAC CGAACCTGCA
    AGAGGTGTTC CGTTACTTCC TGAGCCCTTG AAGCCTCAAC CTGCGGCTCC TCTTCAAACC
    AGTCCAAATT CCCAACCACC CTGCCAAAAA CTGCCTGAAC CTATTCTACC TACAGCAGTA
    CAGGCCGCAT CCCAGACTCC ACCCTTACAG CAAAATCTAG ACCCTCCTCC ACTCCCTCCT
    CCAAGAAGCC GCTCCTCCCA GAACCTGCCC ACTGACCAAA ATGCTTCTCA GCAGCTTCCA
    GCAAACGGAG TCGTTGGCTT TAAAAGCCAG TTCGACAATG ATCCTTTTGT GGACTTTTCA
    TTTGATCAAC TTCCAAATTC AAAGCTTCAG GCTCCGGTTC CCATATCCTC CTCAAGCCAA
    AAGGAGTCAT TTAAGTTGCC TTCTGCTGTC CATAATAATC CGAGTGGCAT AACTTGCATG
    GCTACATTAC CTCCTGTCCC AGCCCGTACT GGATTCCAAG ACAGCACACG CATTTCACCA
    AACCCTTTTG TAAACAGGGT CAACACCATC AGCCCAACCA ACCCATTCAC AGAAAGATCT
    TCTAATGCTG GGAATCCTTT TACAGTCCCA GAAATGGCCG CACATAATCC ACTTCAAACA
    AGGACTTTAG ATCAGTTTAG TTCTACCAGT GAACCTGAAC TTCTGCACAG CAAACCGACC
    TTTACACTGG GCCCTATGGA AAAAAGCCTT TCCCTACCAA ACAAGTCGTA CTCGTTTAAT
    GATGCCAGTC AAAAAGGTTG GATAACGTTT GACGACGATA ACGGCACCGG CAAAATAAAC
    AGGCATTCTG GCAACAGCTC GACGGTGGAT CATGTAAGTT CAGAAAATGA TCTGTTCCAG
    CTGGGATTTA CTAATGACTG GAGTGTCACC TCCACTGCTT CCTCCCATAC GTTGCCGCTA
    AGGAAGCCCC CTGCCATTCC ATCTCGAACA AAACGTGAAC AGCCATCCAC CAACGTCCCA
    CCAAATAATT CTCCAACTCT AGAGTTTACA GATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946897.1
    CDS99..4697
    Translation

    Target ORF information:

    RefSeq Version XM_018091408.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG TAAGAGGCCA GACTCCTTCA CAGATGGGAC CCCCAGCAAT TGCCCCATGC
    AGCTCAGTTA GACCGACCAT TCCTCCTCGT GCTGGAGTTA TCAGTGCTCC ACAAAGCCAT
    GTTCGGCCTG TTCCTGCTCG AACAACCCCT GAGGTCCCAA ACAAGCCCAC CGAACCTGCA
    AGAGGTGTTC CGTTACTTCC TGAGCCCTTG AAGCCTCAAC CTGCGGCTCC TCTTCAAACC
    AGTCCAAATT CCCAACCACC CTGCCAAAAA CTGCCTGAAC CTATTCTACC TACAGCAGTA
    CAGGCCGCAT CCCAGACTCC ACCCTTACAG CAAAATCTAG ACCCTCCTCC ACTCCCTCCT
    CCAAGAAGCC GCTCCTCCCA GAACCTGCCC ACTGACCAAA ATGCTTCTCA GCAGCTTCCA
    GCAAACGGAG TCGTTGGCTT TAAAAGCCAG TTCGACAATG ATCCTTTTGT GGACTTTTCA
    TTTGATCAAC TTCCAAATTC AAAGCTTCAG GCTCCGGTTC CCATATCCTC CTCAAGCCAA
    AAGGAGTCAT TTAAGTTGCC TTCTGCTGTC CATAATAATC CGAGTGGCAT AACTTGCATG
    GCTACATTAC CTCCTGTCCC AGCCCGTACT GGATTCCAAG ACAGCACACG CATTTCACCA
    AACCCTTTTG TAAACAGGGT CAACACCATC AGCCCAACCA ACCCATTCAC AGAAAGATCT
    TCTAATGCTG GGAATCCTTT TACAGTCCCA GAAATGGCCG CACATAATCC ACTTCAAACA
    AGGACTTTAG ATCAGTTTAG TTCTACCAGT GAACCTGAAC TTCTGCACAG CAAACCGACC
    TTTACACTGG GCCCTATGGA AAAAAGCCTT TCCCTACCAA ACAAGTCGTA CTCGTTTAAT
    GATGCCAGTC AAAAAGGTTG GATAACGTTT GACGACGATA ACGGCACCGG CAAAATAAAC
    AGGCATTCTG GCAACAGCTC GACGGTGGAT CATGTAAGTT CAGAAAATGA TCTGTTCCAG
    CTGGGATTTA CTAATGACTG GAGTGTCACC TCCACTGCTT CCTCCCATAC GTTGCCGCTA
    AGGAAGCCCC CTGCCATTCC ATCTCGAACA AAACGTGAAC AGCCATCCAC CAACGTCCCA
    CCAAATAATT CTCCAACTCT AGAGTTTACA GATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06435
    Clone ID Related Accession (Same CDS sequence) XM_004912146.3 , XM_018091404.1 , XM_018091404.2 , XM_004912146.4
    Accession Version XM_004912146.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912146.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGCTTCCAGC AAACGGAGTC
    GTTGGCTTTA AAAGCCAGTT CGACAATGAT CCTTTTGTGG ACTTTTCATT TGATCAACTT
    CCAAATTCAA AGCTTCAGGC TCCGGTTCCC ATATCCTCCT CAAGCCAAAA GGAGTCATTT
    AAGTTGCCTT CTGCTGTCCA TAATAATCCG AGTGGCATAA CTTGCATGGC TACATTACCT
    CCTGTCCCAG CCCGTACTGG ATTCCAAGAC AGCACACGCA TTTCACCAAA CCCTTTTGTA
    AACAGGGTCA ACACCATCAG CCCAACCAAC CCATTCACAG AAAGATCTTC TAATGCTGGG
    AATCCTTTTA CAGTCCCAGA AATGGCCGCA CATAATCCAC TTCAAACAAG GACTTTAGAT
    CAGTTTAGTT CTACCAGTGA ACCTGAACTT CTGCACAGCA AACCGACCTT TACACTGGGC
    CCTATGGAAA AAAGCCTTTC CCTACCAAAC AAGTCGTACT CGTTTAATGA TGCCAGTCAA
    AAAGGTTGGA TAACGTTTGA CGACGATAAC GGCACCGGCA AAATAAACAG GCATTCTGGC
    AACAGCTCGA CGGTGGATCA TGTAAGTTCA GAAAATGATC TGTTCCAGCT GGGATTTACT
    AATGACTGGA GTGTCACCTC CACTGCTTCC TCCCATACGT TGCCGCTAAG GAAGCCCCCT
    GCCATTCCAT CTCGAACAAA ACGTGAACAG CCATCCACCA ACGTCCCACC AAATAATTCT
    CCAACTCTAG AGTTTACAGA TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912203.1
    CDS299..4945
    Translation

    Target ORF information:

    RefSeq Version XM_004912146.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912146.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGCTTCCAGC AAACGGAGTC
    GTTGGCTTTA AAAGCCAGTT CGACAATGAT CCTTTTGTGG ACTTTTCATT TGATCAACTT
    CCAAATTCAA AGCTTCAGGC TCCGGTTCCC ATATCCTCCT CAAGCCAAAA GGAGTCATTT
    AAGTTGCCTT CTGCTGTCCA TAATAATCCG AGTGGCATAA CTTGCATGGC TACATTACCT
    CCTGTCCCAG CCCGTACTGG ATTCCAAGAC AGCACACGCA TTTCACCAAA CCCTTTTGTA
    AACAGGGTCA ACACCATCAG CCCAACCAAC CCATTCACAG AAAGATCTTC TAATGCTGGG
    AATCCTTTTA CAGTCCCAGA AATGGCCGCA CATAATCCAC TTCAAACAAG GACTTTAGAT
    CAGTTTAGTT CTACCAGTGA ACCTGAACTT CTGCACAGCA AACCGACCTT TACACTGGGC
    CCTATGGAAA AAAGCCTTTC CCTACCAAAC AAGTCGTACT CGTTTAATGA TGCCAGTCAA
    AAAGGTTGGA TAACGTTTGA CGACGATAAC GGCACCGGCA AAATAAACAG GCATTCTGGC
    AACAGCTCGA CGGTGGATCA TGTAAGTTCA GAAAATGATC TGTTCCAGCT GGGATTTACT
    AATGACTGGA GTGTCACCTC CACTGCTTCC TCCCATACGT TGCCGCTAAG GAAGCCCCCT
    GCCATTCCAT CTCGAACAAA ACGTGAACAG CCATCCACCA ACGTCCCACC AAATAATTCT
    CCAACTCTAG AGTTTACAGA TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06435
    Clone ID Related Accession (Same CDS sequence) XM_004912146.3 , XM_018091404.1 , XM_018091404.2 , XM_004912146.4
    Accession Version XM_018091404.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGCTTCCAGC AAACGGAGTC
    GTTGGCTTTA AAAGCCAGTT CGACAATGAT CCTTTTGTGG ACTTTTCATT TGATCAACTT
    CCAAATTCAA AGCTTCAGGC TCCGGTTCCC ATATCCTCCT CAAGCCAAAA GGAGTCATTT
    AAGTTGCCTT CTGCTGTCCA TAATAATCCG AGTGGCATAA CTTGCATGGC TACATTACCT
    CCTGTCCCAG CCCGTACTGG ATTCCAAGAC AGCACACGCA TTTCACCAAA CCCTTTTGTA
    AACAGGGTCA ACACCATCAG CCCAACCAAC CCATTCACAG AAAGATCTTC TAATGCTGGG
    AATCCTTTTA CAGTCCCAGA AATGGCCGCA CATAATCCAC TTCAAACAAG GACTTTAGAT
    CAGTTTAGTT CTACCAGTGA ACCTGAACTT CTGCACAGCA AACCGACCTT TACACTGGGC
    CCTATGGAAA AAAGCCTTTC CCTACCAAAC AAGTCGTACT CGTTTAATGA TGCCAGTCAA
    AAAGGTTGGA TAACGTTTGA CGACGATAAC GGCACCGGCA AAATAAACAG GCATTCTGGC
    AACAGCTCGA CGGTGGATCA TGTAAGTTCA GAAAATGATC TGTTCCAGCT GGGATTTACT
    AATGACTGGA GTGTCACCTC CACTGCTTCC TCCCATACGT TGCCGCTAAG GAAGCCCCCT
    GCCATTCCAT CTCGAACAAA ACGTGAACAG CCATCCACCA ACGTCCCACC AAATAATTCT
    CCAACTCTAG AGTTTACAGA TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946893.1
    CDS99..4745
    Translation

    Target ORF information:

    RefSeq Version XM_018091404.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091404.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGGGCTAGGA GTCCAGCACC AGTTAGAAAG
    GAGGTTGGAG GTCCAGGGGC TCCTCCAAGC CCAGGAGTAG CTCGGAGAGA TGTGGAAGTA
    AGAGGCCAGA CTCCTTCACA GATGGGACCC CCAGCAATTG CCCCATGCAG CTCAGTTAGA
    CCGACCATTC CTCCTCGTGC TGGAGTTATC AGTGCTCCAC AAAGCCATGT TCGGCCTGTT
    CCTGCTCGAA CAACCCCTGA GGTCCCAAAC AAGCCCACCG AACCTGCAAG AGGTGTTCCG
    TTACTTCCTG AGCCCTTGAA GCCTCAACCT GCGGCTCCTC TTCAAACCAG TCCAAATTCC
    CAACCACCCT GCCAAAAACT GCCTGAACCT ATTCTACCTA CAGCAGTACA GGCCGCATCC
    CAGACTCCAC CCTTACAGCA AAATCTAGAC CCTCCTCCAC TCCCTCCTCC AAGAAGCCGC
    TCCTCCCAGA ACCTGCCCAC TGACCAAAAT GCTTCTCAGC AGCTTCCAGC AAACGGAGTC
    GTTGGCTTTA AAAGCCAGTT CGACAATGAT CCTTTTGTGG ACTTTTCATT TGATCAACTT
    CCAAATTCAA AGCTTCAGGC TCCGGTTCCC ATATCCTCCT CAAGCCAAAA GGAGTCATTT
    AAGTTGCCTT CTGCTGTCCA TAATAATCCG AGTGGCATAA CTTGCATGGC TACATTACCT
    CCTGTCCCAG CCCGTACTGG ATTCCAAGAC AGCACACGCA TTTCACCAAA CCCTTTTGTA
    AACAGGGTCA ACACCATCAG CCCAACCAAC CCATTCACAG AAAGATCTTC TAATGCTGGG
    AATCCTTTTA CAGTCCCAGA AATGGCCGCA CATAATCCAC TTCAAACAAG GACTTTAGAT
    CAGTTTAGTT CTACCAGTGA ACCTGAACTT CTGCACAGCA AACCGACCTT TACACTGGGC
    CCTATGGAAA AAAGCCTTTC CCTACCAAAC AAGTCGTACT CGTTTAATGA TGCCAGTCAA
    AAAGGTTGGA TAACGTTTGA CGACGATAAC GGCACCGGCA AAATAAACAG GCATTCTGGC
    AACAGCTCGA CGGTGGATCA TGTAAGTTCA GAAAATGATC TGTTCCAGCT GGGATTTACT
    AATGACTGGA GTGTCACCTC CACTGCTTCC TCCCATACGT TGCCGCTAAG GAAGCCCCCT
    GCCATTCCAT CTCGAACAAA ACGTGAACAG CCATCCACCA ACGTCCCACC AAATAATTCT
    CCAACTCTAG AGTTTACAGA TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06436
    Clone ID Related Accession (Same CDS sequence) XM_018091406.1 , XM_018091406.2
    Accession Version XM_018091406.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGCATCCCCC AGAGTGCTAC GGTGTATGTG TGAGAACTAC
    TATAGGTATG CAAAGCCGAG GAAAATCCGC ATCTGTGTCG GCACTTGGAA CGTCAACGGT
    GGGAAGCAAT TCCGAAGCAT CGCTTACAAG AATTCAACAC TGACAGACTG GCTGTTAGAT
    GCACCAAGAA TTGCCGGTAT TCAGGAATTT CAAGATAGAC GGAGCAAGCC AGTTGATATA
    TTTGCCATTG GATTTGAAGA AATGGTTGAG TTAAATGCAG GAAACATTGT TAATGCAAGC
    ACAGCCAATC AAAAGCTGTG GGCTACAGAA TTGCAGAAGA CCGTATCAAG AGACCATAAA
    TATGTACTCC TGGCATCAGA ACAGCTTGTA GGTGTGTGCC TCTACATCTT CATCAGACCC
    CAGCATGCAC CATTTATAAG AGATGTTGCT GTTGATACTG TAAAGACCGG CATGGGTGGC
    GCTACAGGAA ACAAAGGAGC TGTTGCCATA CGAATGCTAT TTCATACAAC CAGCCTGTGC
    TTTGTCTGTA GCCACTTTGC AGCGGGGCAG TCTCAAGTCA AAGAGAGAAA TGAGGACTAT
    AATGAAATAG CCCGAAAACT CAGTTTTCCA ATGGGCAGAA TGCTGTTTTC TCACGACTAT
    GTGTTCTGGT GCGGAGACTT TAATTATCGG ATTGATCTGC CAAATGAAGA GGTCAAAGAA
    ATGATCCGAA ATCAAAACTG GGATTCTCTT ATTTTAGGAG ATCAGCTGAT CAACCAGAAG
    AACAGTGGGC AGGTTTTCCG TGGGTTTCAA GAGGGAAAAA TTAATTTTGC TCCTACATAC
    AAGTATGATC TGTTCTCAGA TGACTATGAC ACAAGTGAGA AGTGTCGGAC TCCAGCATGG
    ACAGACCGCG TCCTCTGGAG AAGAAGGAAA TGGCCTTTTG ACAGATCAGC TGAAGATTTA
    GACCTTCTAA ATTCAAGTTT ACAAGAAGGT AAAAATATTC CATATACCTG GAATCCAGGT
    ACATTACTGC ACTACGGCAG AGTTGAGCTG AAAACGTCAG ATCACAGGCC AATTGTGTCT
    CTGATTGATA TAGATATATT TGAAACTGAT GCAGAAGAGC GGCAGAAAAT TTATAAGGAG
    GTGGTTGCAT CCCAGGGCCC TCCAGATGGC ACCATTATGG TCTCTATAGA GAGCAGCTCA
    CCAGAAGGGA ACTATTTTGA TGATAACTTG ATTGACGAGT TGCTCCAGCA GTTTGCAGCT
    TATGGAGAAG TCATACTTAT AAGGTTCGTT GATGATAAAA TGTGGGTCAC GTTTCTGGAA
    GGAGGCTCAG CGCTTGCTGT TCTAAGCTTA AATGGTTCTC AGGTACAGGG AAAAGCAATC
    CATATCCAAA TGAAAAACCC TGATTGGATG GTAGAGTTAG AAGAGCAGAT CAATTTGGAA
    AAGATTAGTC TCAGTTTGCC TTCTTCAACA AGCTCAACCC TGCTCGGTGA AGATTCAGAA
    ATTTCTGCAG ACTATGACAT GGAAGGAGAT GTTGATGATT ACAGTGCAGA GGTAGAAGAA
    ATTCTTCCCC AGCATTTACA GCCATCTTCA TGTTCTACCC TGGGATCATC TCCTAGCTCT
    TCTCCTCGCT CAAGCCCTTG TCCGTCACCA ACAATGCCAG AAGGCCCTCC TCCATCACTC
    CCTGTACGAC CAAGTCGTGC CCCCCAGAAA ACTCCAGGGC CCCCTGTATC TTCATCTCAA
    GCTGAATTTC AACCAGTGGT ACAACAGAAT GCATCATTAA CTAGTGAACC AAAGCGCCCA
    CCGCCTCCCC GCCCTGGTGT GCCCCCTGCA CGCCCAGCAC CACCACAGCG TCCACCTCCT
    CCATCAGGGG CTAGGAGTCC AGCACCAGTT AGAAAGGAGG TTGGAGGTCC AGGGGCTCCT
    CCAAGCCCAG GAGTAGCTCG GAGAGATGTG GAAGTAAGAG GCCAGACTCC TTCACAGATG
    GGACCCCCAG CAATTGCCCC ATGCAGCTCA GTTAGACCGA CCATTCCTCC TCGTGCTGGA
    GTTATCAGTG CTCCACAAAG CCATGTTCGG CCTGTTCCTG CTCGAACAAC CCCTGAGGTC
    CCAAACAAGC CCACCGAACC TGCAAGAGGT GTTCCGTTAC TTCCTGAGCC CTTGAAGCCT
    CAACCTGCGG CTCCTCTTCA AACCAGTCCA AATTCCCAAC CACCCTGCCA AAAACTGCCT
    GAACCTATTC TACCTACAGC AGTACAGGCC GCATCCCAGA CTCCACCCTT ACAGCAAAAT
    CTAGACCCTC CTCCACTCCC TCCTCCAAGA AGCCGCTCCT CCCAGAACCT GCCCACTGAC
    CAAAATGCTT CTCAGCAGCT TCCAGCAAAC GGAGTCGTTG GCTTTAAAAG CCAGTTCGAC
    AATGATCCTT TTGTGGACTT TTCATTTGAT CAACTTCCAA ATTCAAAGCT TCAGGCTCCG
    GTTCCCATAT CCTCCTCAAG CCAAAAGGAG TCATTTAAGT TGCCTTCTGC TGTCCATAAT
    AATCCGAGTG GCATAACTTG CATGGCTACA TTACCTCCTG TCCCAGCCCG TACTGGATTC
    CAAGACAGCA CACGCATTTC ACCAAACCCT TTTGTAAACA GGGTCAACAC CATCAGCCCA
    ACCAACCCAT TCACAGAAAG ATCTTCTAAT GCTGGGAATC CTTTTACAGT CCCAGAAATG
    GCCGCACATA ATCCACTTCA AACAAGGACT TTAGATCAGT TTAGTTCTAC CAGTGAACCT
    GAACTTCTGC ACAGCAAACC GACCTTTACA CTGGGCCCTA TGGAAAAAAG CCTTTCCCTA
    CCAAACAAGT CGTACTCGTT TAATGATGCC AGTCAAAAAG GTTGGATAAC GTTTGACGAC
    GATAACGGCA CCGGCAAAAT AAACAGGCAT TCTGGCAACA GCTCGACGGT GGATCATGTA
    AGTTCAGAAA ATGATCTGTT CCAGCTGGGA TTTACTAATG ACTGGAGTGT CACCTCCACT
    GCTTCCTCCC ATACGTTGCC GCTAAGGAAG CCCCCTGCCA TTCCATCTCG AACAAAACGT
    GAACAGCCAT CCACCAACGT CCCACCAAAT AATTCTCCAA CTCTAGAGTT TACAGATCGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946895.1
    CDS98..4720
    Translation

    Target ORF information:

    RefSeq Version XM_018091406.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091406.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGCATCCCCC AGAGTGCTAC GGTGTATGTG TGAGAACTAC
    TATAGGTATG CAAAGCCGAG GAAAATCCGC ATCTGTGTCG GCACTTGGAA CGTCAACGGT
    GGGAAGCAAT TCCGAAGCAT CGCTTACAAG AATTCAACAC TGACAGACTG GCTGTTAGAT
    GCACCAAGAA TTGCCGGTAT TCAGGAATTT CAAGATAGAC GGAGCAAGCC AGTTGATATA
    TTTGCCATTG GATTTGAAGA AATGGTTGAG TTAAATGCAG GAAACATTGT TAATGCAAGC
    ACAGCCAATC AAAAGCTGTG GGCTACAGAA TTGCAGAAGA CCGTATCAAG AGACCATAAA
    TATGTACTCC TGGCATCAGA ACAGCTTGTA GGTGTGTGCC TCTACATCTT CATCAGACCC
    CAGCATGCAC CATTTATAAG AGATGTTGCT GTTGATACTG TAAAGACCGG CATGGGTGGC
    GCTACAGGAA ACAAAGGAGC TGTTGCCATA CGAATGCTAT TTCATACAAC CAGCCTGTGC
    TTTGTCTGTA GCCACTTTGC AGCGGGGCAG TCTCAAGTCA AAGAGAGAAA TGAGGACTAT
    AATGAAATAG CCCGAAAACT CAGTTTTCCA ATGGGCAGAA TGCTGTTTTC TCACGACTAT
    GTGTTCTGGT GCGGAGACTT TAATTATCGG ATTGATCTGC CAAATGAAGA GGTCAAAGAA
    ATGATCCGAA ATCAAAACTG GGATTCTCTT ATTTTAGGAG ATCAGCTGAT CAACCAGAAG
    AACAGTGGGC AGGTTTTCCG TGGGTTTCAA GAGGGAAAAA TTAATTTTGC TCCTACATAC
    AAGTATGATC TGTTCTCAGA TGACTATGAC ACAAGTGAGA AGTGTCGGAC TCCAGCATGG
    ACAGACCGCG TCCTCTGGAG AAGAAGGAAA TGGCCTTTTG ACAGATCAGC TGAAGATTTA
    GACCTTCTAA ATTCAAGTTT ACAAGAAGGT AAAAATATTC CATATACCTG GAATCCAGGT
    ACATTACTGC ACTACGGCAG AGTTGAGCTG AAAACGTCAG ATCACAGGCC AATTGTGTCT
    CTGATTGATA TAGATATATT TGAAACTGAT GCAGAAGAGC GGCAGAAAAT TTATAAGGAG
    GTGGTTGCAT CCCAGGGCCC TCCAGATGGC ACCATTATGG TCTCTATAGA GAGCAGCTCA
    CCAGAAGGGA ACTATTTTGA TGATAACTTG ATTGACGAGT TGCTCCAGCA GTTTGCAGCT
    TATGGAGAAG TCATACTTAT AAGGTTCGTT GATGATAAAA TGTGGGTCAC GTTTCTGGAA
    GGAGGCTCAG CGCTTGCTGT TCTAAGCTTA AATGGTTCTC AGGTACAGGG AAAAGCAATC
    CATATCCAAA TGAAAAACCC TGATTGGATG GTAGAGTTAG AAGAGCAGAT CAATTTGGAA
    AAGATTAGTC TCAGTTTGCC TTCTTCAACA AGCTCAACCC TGCTCGGTGA AGATTCAGAA
    ATTTCTGCAG ACTATGACAT GGAAGGAGAT GTTGATGATT ACAGTGCAGA GGTAGAAGAA
    ATTCTTCCCC AGCATTTACA GCCATCTTCA TGTTCTACCC TGGGATCATC TCCTAGCTCT
    TCTCCTCGCT CAAGCCCTTG TCCGTCACCA ACAATGCCAG AAGGCCCTCC TCCATCACTC
    CCTGTACGAC CAAGTCGTGC CCCCCAGAAA ACTCCAGGGC CCCCTGTATC TTCATCTCAA
    GCTGAATTTC AACCAGTGGT ACAACAGAAT GCATCATTAA CTAGTGAACC AAAGCGCCCA
    CCGCCTCCCC GCCCTGGTGT GCCCCCTGCA CGCCCAGCAC CACCACAGCG TCCACCTCCT
    CCATCAGGGG CTAGGAGTCC AGCACCAGTT AGAAAGGAGG TTGGAGGTCC AGGGGCTCCT
    CCAAGCCCAG GAGTAGCTCG GAGAGATGTG GAAGTAAGAG GCCAGACTCC TTCACAGATG
    GGACCCCCAG CAATTGCCCC ATGCAGCTCA GTTAGACCGA CCATTCCTCC TCGTGCTGGA
    GTTATCAGTG CTCCACAAAG CCATGTTCGG CCTGTTCCTG CTCGAACAAC CCCTGAGGTC
    CCAAACAAGC CCACCGAACC TGCAAGAGGT GTTCCGTTAC TTCCTGAGCC CTTGAAGCCT
    CAACCTGCGG CTCCTCTTCA AACCAGTCCA AATTCCCAAC CACCCTGCCA AAAACTGCCT
    GAACCTATTC TACCTACAGC AGTACAGGCC GCATCCCAGA CTCCACCCTT ACAGCAAAAT
    CTAGACCCTC CTCCACTCCC TCCTCCAAGA AGCCGCTCCT CCCAGAACCT GCCCACTGAC
    CAAAATGCTT CTCAGCAGCT TCCAGCAAAC GGAGTCGTTG GCTTTAAAAG CCAGTTCGAC
    AATGATCCTT TTGTGGACTT TTCATTTGAT CAACTTCCAA ATTCAAAGCT TCAGGCTCCG
    GTTCCCATAT CCTCCTCAAG CCAAAAGGAG TCATTTAAGT TGCCTTCTGC TGTCCATAAT
    AATCCGAGTG GCATAACTTG CATGGCTACA TTACCTCCTG TCCCAGCCCG TACTGGATTC
    CAAGACAGCA CACGCATTTC ACCAAACCCT TTTGTAAACA GGGTCAACAC CATCAGCCCA
    ACCAACCCAT TCACAGAAAG ATCTTCTAAT GCTGGGAATC CTTTTACAGT CCCAGAAATG
    GCCGCACATA ATCCACTTCA AACAAGGACT TTAGATCAGT TTAGTTCTAC CAGTGAACCT
    GAACTTCTGC ACAGCAAACC GACCTTTACA CTGGGCCCTA TGGAAAAAAG CCTTTCCCTA
    CCAAACAAGT CGTACTCGTT TAATGATGCC AGTCAAAAAG GTTGGATAAC GTTTGACGAC
    GATAACGGCA CCGGCAAAAT AAACAGGCAT TCTGGCAACA GCTCGACGGT GGATCATGTA
    AGTTCAGAAA ATGATCTGTT CCAGCTGGGA TTTACTAATG ACTGGAGTGT CACCTCCACT
    GCTTCCTCCC ATACGTTGCC GCTAAGGAAG CCCCCTGCCA TTCCATCTCG AACAAAACGT
    GAACAGCCAT CCACCAACGT CCCACCAAAT AATTCTCCAA CTCTAGAGTT TACAGATCGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33901
    Clone ID Related Accession (Same CDS sequence) XM_018091405.2 , XM_018091405.1
    Accession Version XM_018091405.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGCTGA AAGATGGAGC ACGATCTGTG
    ACCAGAACCA TTCAGAATAA TTTTTTTGAT GGCTCCAAAC AAGAAGCGAT AGATGTTTTG
    CTTCTGGGAA ATACCCTAAA TAGTGATCTA GCCGACAAAG CAAGGGCGCT TCTGACCACT
    GGAAGTTTAC ATGTGTCAGA ACTGATGCTG CAGTCAGCAT CCCCCAGAGT GCTACGGTGT
    ATGTGTGAGA ACTACTATAG GTATGCAAAG CCGAGGAAAA TCCGCATCTG TGTCGGCACT
    TGGAACGTCA ACGGTGGGAA GCAATTCCGA AGCATCGCTT ACAAGAATTC AACACTGACA
    GACTGGCTGT TAGATGCACC AAGAATTGCC GGTATTCAGG AATTTCAAGA TAGACGGAGC
    AAGCCAGTTG ATATATTTGC CATTGGATTT GAAGAAATGG TTGAGTTAAA TGCAGGAAAC
    ATTGTTAATG CAAGCACAGC CAATCAAAAG CTGTGGGCTA CAGAATTGCA GAAGACCGTA
    TCAAGAGACC ATAAATATGT ACTCCTGGCA TCAGAACAGC TTGTAGGTGT GTGCCTCTAC
    ATCTTCATCA GACCCCAGCA TGCACCATTT ATAAGAGATG TTGCTGTTGA TACTGTAAAG
    ACCGGCATGG GTGGCGCTAC AGGAAACAAA GGAGCTGTTG CCATACGAAT GCTATTTCAT
    ACAACCAGCC TGTGCTTTGT CTGTAGCCAC TTTGCAGCGG GGCAGTCTCA AGTCAAAGAG
    AGAAATGAGG ACTATAATGA AATAGCCCGA AAACTCAGTT TTCCAATGGG CAGAATGCTG
    TTTTCTCACG ACTATGTGTT CTGGTGCGGA GACTTTAATT ATCGGATTGA TCTGCCAAAT
    GAAGAGGTCA AAGAAATGAT CCGAAATCAA AACTGGGATT CTCTTATTTT AGGAGATCAG
    CTGATCAACC AGAAGAACAG TGGGCAGGTT TTCCGTGGGT TTCAAGAGGG AAAAATTAAT
    TTTGCTCCTA CATACAAGTA TGATCTGTTC TCAGATGACT ATGACACAAG TGAGAAGTGT
    CGGACTCCAG CATGGACAGA CCGCGTCCTC TGGAGAAGAA GGAAATGGCC TTTTGACAGA
    TCAGCTGAAG ATTTAGACCT TCTAAATTCA AGTTTACAAG AAGGTAAAAA TATTCCATAT
    ACCTGGAATC CAGGTACATT ACTGCACTAC GGCAGAGTTG AGCTGAAAAC GTCAGATCAC
    AGGCCAATTG TGTCTCTGAT TGATATAGAT ATATTTGAAA CTGATGCAGA AGAGCGGCAG
    AAAATTTATA AGGAGGTGGT TGCATCCCAG GGCCCTCCAG ATGGCACCAT TATGGTCTCT
    ATAGAGAGCA GCTCACCAGA AGGGAACTAT TTTGATGATA ACTTGATTGA CGAGTTGCTC
    CAGCAGTTTG CAGCTTATGG AGAAGTCATA CTTATAAGGT TCGTTGATGA TAAAATGTGG
    GTCACGTTTC TGGAAGGAGG CTCAGCGCTT GCTGTTCTAA GCTTAAATGG TTCTCAGGTA
    CAGGGAAAAG CAATCCATAT CCAAATGAAA AACCCTGATT GGATGGTAGA GTTAGAAGAG
    CAGATCAATT TGGAAAAGAT TAGTCTCAGT TTGCCTTCTT CAACAAGCTC AACCCTGCTC
    GGTGAAGATT CAGAAATTTC TGCAGACTAT GACATGGAAG GAGATGTTGA TGATTACAGT
    GCAGAGGTAG AAGAAATTCT TCCCCAGCAT TTACAGCCAT CTTCATGTTC TACCCTGGGA
    TCATCTCCTA GCTCTTCTCC TCGCTCAAGC CCTTGTCCGT CACCAACAAT GCCAGAAGGC
    CCTCCTCCAT CACTCCCTGT ACGACCAAGT CGTGCCCCCC AGAAAACTCC AGGGCCCCCT
    GTATCTTCAT CTCAAGCTGA ATTTCAACCA GTGGTACAAC AGAATGCATC ATTAACTAGT
    GAACCAAAGC GCCCACCGCC TCCCCGCCCT GGTGTGCCCC CTGCACGCCC AGCACCACCA
    CAGCGTCCAC CTCCTCCATC AGGGGCTAGG AGTCCAGCAC CAGTTAGAAA GGAGGTTGGA
    GGTCCAGGGG CTCCTCCAAG CCCAGGAGTA GCTCGGAGAG ATGTGGAAGT AAGAGGCCAG
    ACTCCTTCAC AGATGGGACC CCCAGCAATT GCCCCATGCA GCTCAGTTAG ACCGACCATT
    CCTCCTCGTG CTGGAGTTAT CAGTGCTCCA CAAAGCCATG TTCGGCCTGT TCCTGCTCGA
    ACAACCCCTG AGGTCCCAAA CAAGCCCACC GAACCTGCAA GAGGTGTTCC GTTACTTCCT
    GAGCCCTTGA AGCCTCAACC TGCGGCTCCT CTTCAAACCA GTCCAAATTC CCAACCACCC
    TGCCAAAAAC TGCCTGAACC TATTCTACCT ACAGCAGTAC AGGCCGCATC CCAGACTCCA
    CCCTTACAGC AAAATCTAGA CCCTCCTCCA CTCCCTCCTC CAAGAAGCCG CTCCTCCCAG
    AACCTGCCCA CTGACCAAAA TGCTTCTCAG CAGCTTCCAG CAAACGGAGT CGTTGGCTTT
    AAAAGCCAGT TCGACAATGA TCCTTTTGTG GACTTTTCAT TTGATCAACT TCCAAATTCA
    AAGCTTCAGG CTCCGGTTCC CATATCCTCC TCAAGCCAAA AGGAGTCATT TAAGTTGCCT
    TCTGCTGTCC ATAATAATCC GAGTGGCATA ACTTGCATGG CTACATTACC TCCTGTCCCA
    GCCCGTACTG GATTCCAAGA CAGCACACGC ATTTCACCAA ACCCTTTTGT AAACAGGGTC
    AACACCATCA GCCCAACCAA CCCATTCACA GAAAGATCTT CTAATGCTGG GAATCCTTTT
    ACAGTCCCAG AAATGGCCGC ACATAATCCA CTTCAAACAA GGACTTTAGA TCAGTTTAGT
    TCTACCAGTG AACCTGAACT TCTGCACAGC AAACCGACCT TTACACTGGG CCCTATGGAA
    AAAAGCCTTT CCCTACCAAA CAAGTCGTAC TCGTTTAATG ATGCCAGTCA AAAAGGTTGG
    ATAACGTTTG ACGACGATAA CGGCACCGGC AAAATAAACA GGCATTCTGG CAACAGCTCG
    ACGGTGGATC ATGTAAGTTC AGAAAATGAT CTGTTCCAGC TGGGATTTAC TAATGACTGG
    AGTGTCACCT CCACTGCTTC CTCCCATACG TTGCCGCTAA GGAAGCCCCC TGCCATTCCA
    TCTCGAACAA AACGTGAACA GCCATCCACC AACGTCCCAC CAAATAATTC TCCAACTCTA
    GAGTTTACAG ATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946894.1
    CDS99..4736
    Translation

    Target ORF information:

    RefSeq Version XM_018091405.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091405.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGCTGA AAGATGGAGC ACGATCTGTG
    ACCAGAACCA TTCAGAATAA TTTTTTTGAT GGCTCCAAAC AAGAAGCGAT AGATGTTTTG
    CTTCTGGGAA ATACCCTAAA TAGTGATCTA GCCGACAAAG CAAGGGCGCT TCTGACCACT
    GGAAGTTTAC ATGTGTCAGA ACTGATGCTG CAGTCAGCAT CCCCCAGAGT GCTACGGTGT
    ATGTGTGAGA ACTACTATAG GTATGCAAAG CCGAGGAAAA TCCGCATCTG TGTCGGCACT
    TGGAACGTCA ACGGTGGGAA GCAATTCCGA AGCATCGCTT ACAAGAATTC AACACTGACA
    GACTGGCTGT TAGATGCACC AAGAATTGCC GGTATTCAGG AATTTCAAGA TAGACGGAGC
    AAGCCAGTTG ATATATTTGC CATTGGATTT GAAGAAATGG TTGAGTTAAA TGCAGGAAAC
    ATTGTTAATG CAAGCACAGC CAATCAAAAG CTGTGGGCTA CAGAATTGCA GAAGACCGTA
    TCAAGAGACC ATAAATATGT ACTCCTGGCA TCAGAACAGC TTGTAGGTGT GTGCCTCTAC
    ATCTTCATCA GACCCCAGCA TGCACCATTT ATAAGAGATG TTGCTGTTGA TACTGTAAAG
    ACCGGCATGG GTGGCGCTAC AGGAAACAAA GGAGCTGTTG CCATACGAAT GCTATTTCAT
    ACAACCAGCC TGTGCTTTGT CTGTAGCCAC TTTGCAGCGG GGCAGTCTCA AGTCAAAGAG
    AGAAATGAGG ACTATAATGA AATAGCCCGA AAACTCAGTT TTCCAATGGG CAGAATGCTG
    TTTTCTCACG ACTATGTGTT CTGGTGCGGA GACTTTAATT ATCGGATTGA TCTGCCAAAT
    GAAGAGGTCA AAGAAATGAT CCGAAATCAA AACTGGGATT CTCTTATTTT AGGAGATCAG
    CTGATCAACC AGAAGAACAG TGGGCAGGTT TTCCGTGGGT TTCAAGAGGG AAAAATTAAT
    TTTGCTCCTA CATACAAGTA TGATCTGTTC TCAGATGACT ATGACACAAG TGAGAAGTGT
    CGGACTCCAG CATGGACAGA CCGCGTCCTC TGGAGAAGAA GGAAATGGCC TTTTGACAGA
    TCAGCTGAAG ATTTAGACCT TCTAAATTCA AGTTTACAAG AAGGTAAAAA TATTCCATAT
    ACCTGGAATC CAGGTACATT ACTGCACTAC GGCAGAGTTG AGCTGAAAAC GTCAGATCAC
    AGGCCAATTG TGTCTCTGAT TGATATAGAT ATATTTGAAA CTGATGCAGA AGAGCGGCAG
    AAAATTTATA AGGAGGTGGT TGCATCCCAG GGCCCTCCAG ATGGCACCAT TATGGTCTCT
    ATAGAGAGCA GCTCACCAGA AGGGAACTAT TTTGATGATA ACTTGATTGA CGAGTTGCTC
    CAGCAGTTTG CAGCTTATGG AGAAGTCATA CTTATAAGGT TCGTTGATGA TAAAATGTGG
    GTCACGTTTC TGGAAGGAGG CTCAGCGCTT GCTGTTCTAA GCTTAAATGG TTCTCAGGTA
    CAGGGAAAAG CAATCCATAT CCAAATGAAA AACCCTGATT GGATGGTAGA GTTAGAAGAG
    CAGATCAATT TGGAAAAGAT TAGTCTCAGT TTGCCTTCTT CAACAAGCTC AACCCTGCTC
    GGTGAAGATT CAGAAATTTC TGCAGACTAT GACATGGAAG GAGATGTTGA TGATTACAGT
    GCAGAGGTAG AAGAAATTCT TCCCCAGCAT TTACAGCCAT CTTCATGTTC TACCCTGGGA
    TCATCTCCTA GCTCTTCTCC TCGCTCAAGC CCTTGTCCGT CACCAACAAT GCCAGAAGGC
    CCTCCTCCAT CACTCCCTGT ACGACCAAGT CGTGCCCCCC AGAAAACTCC AGGGCCCCCT
    GTATCTTCAT CTCAAGCTGA ATTTCAACCA GTGGTACAAC AGAATGCATC ATTAACTAGT
    GAACCAAAGC GCCCACCGCC TCCCCGCCCT GGTGTGCCCC CTGCACGCCC AGCACCACCA
    CAGCGTCCAC CTCCTCCATC AGGGGCTAGG AGTCCAGCAC CAGTTAGAAA GGAGGTTGGA
    GGTCCAGGGG CTCCTCCAAG CCCAGGAGTA GCTCGGAGAG ATGTGGAAGT AAGAGGCCAG
    ACTCCTTCAC AGATGGGACC CCCAGCAATT GCCCCATGCA GCTCAGTTAG ACCGACCATT
    CCTCCTCGTG CTGGAGTTAT CAGTGCTCCA CAAAGCCATG TTCGGCCTGT TCCTGCTCGA
    ACAACCCCTG AGGTCCCAAA CAAGCCCACC GAACCTGCAA GAGGTGTTCC GTTACTTCCT
    GAGCCCTTGA AGCCTCAACC TGCGGCTCCT CTTCAAACCA GTCCAAATTC CCAACCACCC
    TGCCAAAAAC TGCCTGAACC TATTCTACCT ACAGCAGTAC AGGCCGCATC CCAGACTCCA
    CCCTTACAGC AAAATCTAGA CCCTCCTCCA CTCCCTCCTC CAAGAAGCCG CTCCTCCCAG
    AACCTGCCCA CTGACCAAAA TGCTTCTCAG CAGCTTCCAG CAAACGGAGT CGTTGGCTTT
    AAAAGCCAGT TCGACAATGA TCCTTTTGTG GACTTTTCAT TTGATCAACT TCCAAATTCA
    AAGCTTCAGG CTCCGGTTCC CATATCCTCC TCAAGCCAAA AGGAGTCATT TAAGTTGCCT
    TCTGCTGTCC ATAATAATCC GAGTGGCATA ACTTGCATGG CTACATTACC TCCTGTCCCA
    GCCCGTACTG GATTCCAAGA CAGCACACGC ATTTCACCAA ACCCTTTTGT AAACAGGGTC
    AACACCATCA GCCCAACCAA CCCATTCACA GAAAGATCTT CTAATGCTGG GAATCCTTTT
    ACAGTCCCAG AAATGGCCGC ACATAATCCA CTTCAAACAA GGACTTTAGA TCAGTTTAGT
    TCTACCAGTG AACCTGAACT TCTGCACAGC AAACCGACCT TTACACTGGG CCCTATGGAA
    AAAAGCCTTT CCCTACCAAA CAAGTCGTAC TCGTTTAATG ATGCCAGTCA AAAAGGTTGG
    ATAACGTTTG ACGACGATAA CGGCACCGGC AAAATAAACA GGCATTCTGG CAACAGCTCG
    ACGGTGGATC ATGTAAGTTC AGAAAATGAT CTGTTCCAGC TGGGATTTAC TAATGACTGG
    AGTGTCACCT CCACTGCTTC CTCCCATACG TTGCCGCTAA GGAAGCCCCC TGCCATTCCA
    TCTCGAACAA AACGTGAACA GCCATCCACC AACGTCCCAC CAAATAATTC TCCAACTCTA
    GAGTTTACAG ATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33902
    Clone ID Related Accession (Same CDS sequence) XM_018091407.2 , XM_018091407.1
    Accession Version XM_018091407.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4608bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGTCCAGGGG CTCCTCCAAG CCCAGGAGTA
    GCTCGGAGAG ATGTGGAAGT AAGAGGCCAG ACTCCTTCAC AGATGGGACC CCCAGCAATT
    GCCCCATGCA GCTCAGTTAG ACCGACCATT CCTCCTCGTG CTGGAGTTAT CAGTGCTCCA
    CAAAGCCATG TTCGGCCTGT TCCTGCTCGA ACAACCCCTG AGGTCCCAAA CAAGCCCACC
    GAACCTGCAA GAGGTGTTCC GTTACTTCCT GAGCCCTTGA AGCCTCAACC TGCGGCTCCT
    CTTCAAACCA GTCCAAATTC CCAACCACCC TGCCAAAAAC TGCCTGAACC TATTCTACCT
    ACAGCAGTAC AGGCCGCATC CCAGACTCCA CCCTTACAGC AAAATCTAGA CCCTCCTCCA
    CTCCCTCCTC CAAGAAGCCG CTCCTCCCAG AACCTGCCCA CTGACCAAAA TGCTTCTCAG
    CAGCTTCCAG CAAACGGAGT CGTTGGCTTT AAAAGCCAGT TCGACAATGA TCCTTTTGTG
    GACTTTTCAT TTGATCAACT TCCAAATTCA AAGCTTCAGG CTCCGGTTCC CATATCCTCC
    TCAAGCCAAA AGGAGTCATT TAAGTTGCCT TCTGCTGTCC ATAATAATCC GAGTGGCATA
    ACTTGCATGG CTACATTACC TCCTGTCCCA GCCCGTACTG GATTCCAAGA CAGCACACGC
    ATTTCACCAA ACCCTTTTGT AAACAGGGTC AACACCATCA GCCCAACCAA CCCATTCACA
    GAAAGATCTT CTAATGCTGG GAATCCTTTT ACAGTCCCAG AAATGGCCGC ACATAATCCA
    CTTCAAACAA GGACTTTAGA TCAGTTTAGT TCTACCAGTG AACCTGAACT TCTGCACAGC
    AAACCGACCT TTACACTGGG CCCTATGGAA AAAAGCCTTT CCCTACCAAA CAAGTCGTAC
    TCGTTTAATG ATGCCAGTCA AAAAGGTTGG ATAACGTTTG ACGACGATAA CGGCACCGGC
    AAAATAAACA GGCATTCTGG CAACAGCTCG ACGGTGGATC ATGTAAGTTC AGAAAATGAT
    CTGTTCCAGC TGGGATTTAC TAATGACTGG AGTGTCACCT CCACTGCTTC CTCCCATACG
    TTGCCGCTAA GGAAGCCCCC TGCCATTCCA TCTCGAACAA AACGTGAACA GCCATCCACC
    AACGTCCCAC CAAATAATTC TCCAACTCTA GAGTTTACAG ATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946896.1
    CDS98..4705
    Translation

    Target ORF information:

    RefSeq Version XM_018091407.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091407.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GGTCCAGGGG CTCCTCCAAG CCCAGGAGTA
    GCTCGGAGAG ATGTGGAAGT AAGAGGCCAG ACTCCTTCAC AGATGGGACC CCCAGCAATT
    GCCCCATGCA GCTCAGTTAG ACCGACCATT CCTCCTCGTG CTGGAGTTAT CAGTGCTCCA
    CAAAGCCATG TTCGGCCTGT TCCTGCTCGA ACAACCCCTG AGGTCCCAAA CAAGCCCACC
    GAACCTGCAA GAGGTGTTCC GTTACTTCCT GAGCCCTTGA AGCCTCAACC TGCGGCTCCT
    CTTCAAACCA GTCCAAATTC CCAACCACCC TGCCAAAAAC TGCCTGAACC TATTCTACCT
    ACAGCAGTAC AGGCCGCATC CCAGACTCCA CCCTTACAGC AAAATCTAGA CCCTCCTCCA
    CTCCCTCCTC CAAGAAGCCG CTCCTCCCAG AACCTGCCCA CTGACCAAAA TGCTTCTCAG
    CAGCTTCCAG CAAACGGAGT CGTTGGCTTT AAAAGCCAGT TCGACAATGA TCCTTTTGTG
    GACTTTTCAT TTGATCAACT TCCAAATTCA AAGCTTCAGG CTCCGGTTCC CATATCCTCC
    TCAAGCCAAA AGGAGTCATT TAAGTTGCCT TCTGCTGTCC ATAATAATCC GAGTGGCATA
    ACTTGCATGG CTACATTACC TCCTGTCCCA GCCCGTACTG GATTCCAAGA CAGCACACGC
    ATTTCACCAA ACCCTTTTGT AAACAGGGTC AACACCATCA GCCCAACCAA CCCATTCACA
    GAAAGATCTT CTAATGCTGG GAATCCTTTT ACAGTCCCAG AAATGGCCGC ACATAATCCA
    CTTCAAACAA GGACTTTAGA TCAGTTTAGT TCTACCAGTG AACCTGAACT TCTGCACAGC
    AAACCGACCT TTACACTGGG CCCTATGGAA AAAAGCCTTT CCCTACCAAA CAAGTCGTAC
    TCGTTTAATG ATGCCAGTCA AAAAGGTTGG ATAACGTTTG ACGACGATAA CGGCACCGGC
    AAAATAAACA GGCATTCTGG CAACAGCTCG ACGGTGGATC ATGTAAGTTC AGAAAATGAT
    CTGTTCCAGC TGGGATTTAC TAATGACTGG AGTGTCACCT CCACTGCTTC CTCCCATACG
    TTGCCGCTAA GGAAGCCCCC TGCCATTCCA TCTCGAACAA AACGTGAACA GCCATCCACC
    AACGTCCCAC CAAATAATTC TCCAACTCTA GAGTTTACAG ATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33903
    Clone ID Related Accession (Same CDS sequence) XM_018091409.1 , XM_018091409.2
    Accession Version XM_018091409.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product synaptojanin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GTAAGAGGCC AGACTCCTTC ACAGATGGGA
    CCCCCAGCAA TTGCCCCATG CAGCTCAGTT AGACCGACCA TTCCTCCTCG TGCTGGAGTT
    ATCAGTGCTC CACAAAGCCA TGTTCGGCCT GTTCCTGCTC GAACAACCCC TGAGGTCCCA
    AACAAGCCCA CCGAACCTGC AAGAGGTGTT CCGTTACTTC CTGAGCCCTT GAAGCCTCAA
    CCTGCGGCTC CTCTTCAAAC CAGTCCAAAT TCCCAACCAC CCTGCCAAAA ACTGCCTGAA
    CCTATTCTAC CTACAGCAGT ACAGGCCGCA TCCCAGACTC CACCCTTACA GCAAAATCTA
    GACCCTCCTC CACTCCCTCC TCCAAGAAGC CGCTCCTCCC AGAACCTGCC CACTGACCAA
    AATGCTTCTC AGCAGCTTCC AGCAAACGGA GTCGTTGGCT TTAAAAGCCA GTTCGACAAT
    GATCCTTTTG TGGACTTTTC ATTTGATCAA CTTCCAAATT CAAAGCTTCA GGCTCCGGTT
    CCCATATCCT CCTCAAGCCA AAAGGAGTCA TTTAAGTTGC CTTCTGCTGT CCATAATAAT
    CCGAGTGGCA TAACTTGCAT GGCTACATTA CCTCCTGTCC CAGCCCGTAC TGGATTCCAA
    GACAGCACAC GCATTTCACC AAACCCTTTT GTAAACAGGG TCAACACCAT CAGCCCAACC
    AACCCATTCA CAGAAAGATC TTCTAATGCT GGGAATCCTT TTACAGTCCC AGAAATGGCC
    GCACATAATC CACTTCAAAC AAGGACTTTA GATCAGTTTA GTTCTACCAG TGAACCTGAA
    CTTCTGCACA GCAAACCGAC CTTTACACTG GGCCCTATGG AAAAAAGCCT TTCCCTACCA
    AACAAGTCGT ACTCGTTTAA TGATGCCAGT CAAAAAGGTT GGATAACGTT TGACGACGAT
    AACGGCACCG GCAAAATAAA CAGGCATTCT GGCAACAGCT CGACGGTGGA TCATGTAAGT
    TCAGAAAATG ATCTGTTCCA GCTGGGATTT ACTAATGACT GGAGTGTCAC CTCCACTGCT
    TCCTCCCATA CGTTGCCGCT AAGGAAGCCC CCTGCCATTC CATCTCGAAC AAAACGTGAA
    CAGCCATCCA CCAACGTCCC ACCAAATAAT TCTCCAACTC TAGAGTTTAC AGATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946898.1
    CDS99..4658
    Translation

    Target ORF information:

    RefSeq Version XM_018091409.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis synaptojanin 1 (synj1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091409.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTTTCA GTAAAGGATA TCGGATCTTT CACAAACTGG ATCCAACCCC ATTCAGCGTT 
    ATTGTGGAGG CCAGAAACAG AGAGGAGTGT CTTTTGTTTG AATCTGGAGC AGTTGCCGTT
    CTGTCATCTG CGGAAAAAGA AGCAATAAAG AACAGTTACA CTAAAGTTAT GGATGCGTAT
    GGGCTGCTGG GGGTTCTGCG ATTAAATCTT GGAGATTCCA TGTCCTATTA TCTAGTGCTG
    GTAACTGGGT GTATGTCAGT AGGGAAAATC CAAGATTCCG AGGTCTTCCG GGTGACTTCC
    ACGGATTTCA TTTCATTGCG AATGGATCCT ATGGCTGAAG ATCGCATGTC AGAAGTGCGC
    AAAGTATTAA ATTCTGGCAG TTTCTATTTT GCTTGGTCTT CTACCGGAGT CAGCTTAGAT
    CTTAGTCTCA ATGCTCACCG TAGCACCCAG GAGCACAGAA CAGACAACAG GTTTTTCTGG
    AACCAGTCTC TTCATTTGCA CCTAAAGCAT TATGGTGTAA ACTGTGATGA GTGGCTGCTG
    CGTCTAATGT GCGGTGGCGT AGAGATTAGG ACTATTTATG CCGGGCACCA GCAGGCCAAG
    GCCTGTATCA TTTCCCGTCT CAGCTGTGAA AGAGCAGGCA CTAGGTTCAA TGTACGAGGA
    ACAAATGATT ATGGGCAAGC TGCTAACTTT GTGGAAACCG AACAGGTTAT ATACCTTGAT
    GATTGCGTCT CTTCCTTTAT ACAGATTCGG GGTTCCATAC CTCTGTTTTG GGAACAACCA
    GGCTTGCAAG TGGGATCGCA CCGTGTTAGA ATGTCAAGGG GATTTGAAGC CAACGCACCA
    GCCTTTGATA GACATTTCCT GACTTTGAAG AGGCTTTATG GAAAACAATT TATAGTAAAT
    TTACTAGGGT CCAAGGAGGG AGAGAGCATG CTGAGTAGAG CCTTTCAGAA TCATTTAAAA
    GCCTCTGAGC ACTCTGCATC TATCCAGATG ATGAATTTTG ACTATCATCA GATGGTTAGA
    GGGGGCAAAG CGGAGAAGCT CCACAGCGTT TTGAAGCCTA AAGTTAACAC CTTCTTGGAG
    GAGTGTGGAT TTTTCTTTCT TGACAAGAAT GGAGTTCACA GGACTCAAAT TGGAACCATC
    AGATCCAACT GCCTTGATTG TCTAGATAGA ACCAACAGTG TACAGGCATT CTTAGGTCTA
    GAGATGCTGT TAAAGCAGTT AGAAGTTCTA GGCTTGGCTG AAAAACCACA ACTAGTCACA
    CGTTTCCAAG AAGTCTTTCG TTCCATGTGG TCAACAAATG GAGACTCCAT AAGCAAGATT
    TACGCAGGCA CTGGTGCCTT AGAAGGAAAG GCTAAGGGCG GAAAGCTGAA AGATGGAGCA
    CGATCTGTGA CCAGAACCAT TCAGAATAAT TTTTTTGATG GCTCCAAACA AGAAGCGATA
    GATGTTTTGC TTCTGGGAAA TACCCTAAAT AGTGATCTAG CCGACAAAGC AAGGGCGCTT
    CTGACCACTG GAAGTTTACA TGTGTCAGAA CTGATGCTGC AGTCAGCATC CCCCAGAGTG
    CTACGGTGTA TGTGTGAGAA CTACTATAGG TATGCAAAGC CGAGGAAAAT CCGCATCTGT
    GTCGGCACTT GGAACGTCAA CGGTGGGAAG CAATTCCGAA GCATCGCTTA CAAGAATTCA
    ACACTGACAG ACTGGCTGTT AGATGCACCA AGAATTGCCG GTATTCAGGA ATTTCAAGAT
    AGACGGAGCA AGCCAGTTGA TATATTTGCC ATTGGATTTG AAGAAATGGT TGAGTTAAAT
    GCAGGAAACA TTGTTAATGC AAGCACAGCC AATCAAAAGC TGTGGGCTAC AGAATTGCAG
    AAGACCGTAT CAAGAGACCA TAAATATGTA CTCCTGGCAT CAGAACAGCT TGTAGGTGTG
    TGCCTCTACA TCTTCATCAG ACCCCAGCAT GCACCATTTA TAAGAGATGT TGCTGTTGAT
    ACTGTAAAGA CCGGCATGGG TGGCGCTACA GGAAACAAAG GAGCTGTTGC CATACGAATG
    CTATTTCATA CAACCAGCCT GTGCTTTGTC TGTAGCCACT TTGCAGCGGG GCAGTCTCAA
    GTCAAAGAGA GAAATGAGGA CTATAATGAA ATAGCCCGAA AACTCAGTTT TCCAATGGGC
    AGAATGCTGT TTTCTCACGA CTATGTGTTC TGGTGCGGAG ACTTTAATTA TCGGATTGAT
    CTGCCAAATG AAGAGGTCAA AGAAATGATC CGAAATCAAA ACTGGGATTC TCTTATTTTA
    GGAGATCAGC TGATCAACCA GAAGAACAGT GGGCAGGTTT TCCGTGGGTT TCAAGAGGGA
    AAAATTAATT TTGCTCCTAC ATACAAGTAT GATCTGTTCT CAGATGACTA TGACACAAGT
    GAGAAGTGTC GGACTCCAGC ATGGACAGAC CGCGTCCTCT GGAGAAGAAG GAAATGGCCT
    TTTGACAGAT CAGCTGAAGA TTTAGACCTT CTAAATTCAA GTTTACAAGA AGGTAAAAAT
    ATTCCATATA CCTGGAATCC AGGTACATTA CTGCACTACG GCAGAGTTGA GCTGAAAACG
    TCAGATCACA GGCCAATTGT GTCTCTGATT GATATAGATA TATTTGAAAC TGATGCAGAA
    GAGCGGCAGA AAATTTATAA GGAGGTGGTT GCATCCCAGG GCCCTCCAGA TGGCACCATT
    ATGGTCTCTA TAGAGAGCAG CTCACCAGAA GGGAACTATT TTGATGATAA CTTGATTGAC
    GAGTTGCTCC AGCAGTTTGC AGCTTATGGA GAAGTCATAC TTATAAGGTT CGTTGATGAT
    AAAATGTGGG TCACGTTTCT GGAAGGAGGC TCAGCGCTTG CTGTTCTAAG CTTAAATGGT
    TCTCAGGTAC AGGGAAAAGC AATCCATATC CAAATGAAAA ACCCTGATTG GATGGTAGAG
    TTAGAAGAGC AGATCAATTT GGAAAAGATT AGTCTCAGTT TGCCTTCTTC AACAAGCTCA
    ACCCTGCTCG GTGAAGATTC AGAAATTTCT GCAGACTATG ACATGGAAGG AGATGTTGAT
    GATTACAGTG CAGAGGTAGA AGAAATTCTT CCCCAGCATT TACAGCCATC TTCATGTTCT
    ACCCTGGGAT CATCTCCTAG CTCTTCTCCT CGCTCAAGCC CTTGTCCGTC ACCAACAATG
    CCAGAAGGCC CTCCTCCATC ACTCCCTGTA CGACCAAGTC GTGCCCCCCA GAAAACTCCA
    GGGCCCCCTG TATCTTCATC TCAAGCTGAA TTTCAACCAG TGGTACAACA GAATGCATCA
    TTAACTAGTG AACCAAAGCG CCCACCGCCT CCCCGCCCTG GTGTGCCCCC TGCACGCCCA
    GCACCACCAC AGCGTCCACC TCCTCCATCA GTAAGAGGCC AGACTCCTTC ACAGATGGGA
    CCCCCAGCAA TTGCCCCATG CAGCTCAGTT AGACCGACCA TTCCTCCTCG TGCTGGAGTT
    ATCAGTGCTC CACAAAGCCA TGTTCGGCCT GTTCCTGCTC GAACAACCCC TGAGGTCCCA
    AACAAGCCCA CCGAACCTGC AAGAGGTGTT CCGTTACTTC CTGAGCCCTT GAAGCCTCAA
    CCTGCGGCTC CTCTTCAAAC CAGTCCAAAT TCCCAACCAC CCTGCCAAAA ACTGCCTGAA
    CCTATTCTAC CTACAGCAGT ACAGGCCGCA TCCCAGACTC CACCCTTACA GCAAAATCTA
    GACCCTCCTC CACTCCCTCC TCCAAGAAGC CGCTCCTCCC AGAACCTGCC CACTGACCAA
    AATGCTTCTC AGCAGCTTCC AGCAAACGGA GTCGTTGGCT TTAAAAGCCA GTTCGACAAT
    GATCCTTTTG TGGACTTTTC ATTTGATCAA CTTCCAAATT CAAAGCTTCA GGCTCCGGTT
    CCCATATCCT CCTCAAGCCA AAAGGAGTCA TTTAAGTTGC CTTCTGCTGT CCATAATAAT
    CCGAGTGGCA TAACTTGCAT GGCTACATTA CCTCCTGTCC CAGCCCGTAC TGGATTCCAA
    GACAGCACAC GCATTTCACC AAACCCTTTT GTAAACAGGG TCAACACCAT CAGCCCAACC
    AACCCATTCA CAGAAAGATC TTCTAATGCT GGGAATCCTT TTACAGTCCC AGAAATGGCC
    GCACATAATC CACTTCAAAC AAGGACTTTA GATCAGTTTA GTTCTACCAG TGAACCTGAA
    CTTCTGCACA GCAAACCGAC CTTTACACTG GGCCCTATGG AAAAAAGCCT TTCCCTACCA
    AACAAGTCGT ACTCGTTTAA TGATGCCAGT CAAAAAGGTT GGATAACGTT TGACGACGAT
    AACGGCACCG GCAAAATAAA CAGGCATTCT GGCAACAGCT CGACGGTGGA TCATGTAAGT
    TCAGAAAATG ATCTGTTCCA GCTGGGATTT ACTAATGACT GGAGTGTCAC CTCCACTGCT
    TCCTCCCATA CGTTGCCGCT AAGGAAGCCC CCTGCCATTC CATCTCGAAC AAAACGTGAA
    CAGCCATCCA CCAACGTCCC ACCAAATAAT TCTCCAACTC TAGAGTTTAC AGATCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.