arhgef4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following arhgef4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa33789 XM_012970967.3
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OXa58893 XM_031902570.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa58891 XM_004917799.4
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa58892 XM_031902569.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa16058 XM_004917799.3
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa16059 XM_002933352.4
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OXa33789 XM_012970967.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa33789
    Clone ID Related Accession (Same CDS sequence) XM_012970967.2 , XM_012970967.3
    Accession Version XM_012970967.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGTCAG GTTACTTATC ACTGGGAAGG ACAGGCAGCT CAGCACTGAG CCAGCACTGC 
    CAGGTACTTG TGCCTGCAAC AAGCAACAAG AGGCTTCTCT CAGCTGAGCT GTGCACCCAG
    TCTGCCCGGA TGAGGACTCG CCAATGCCCC AGTGATGCCA TGAACCCACA CAGCAGCAGT
    GGGAGTTCAG GGCTTTCCTC TGGCTCAGAT TCTGACAGCA GTAGCTGCCT TGGCTGGGGT
    GGTGCTGCTG CTGCTCCTGC TGCTGCTTCT GGGAGAGGAT TGCTGTCCAG ATACTGGAGC
    TTGGAAAGCC TTCACTCTGC TACCGTATTC AATGATGGGT CTACGAACAG ACCTTCGCTG
    TTTGATGACT TGGGGAGTGA TGAGGATTTT TATGAAGAAC TACACGTTTC TGGTCACCGC
    TTTGTTGGAG GGGGCGAGCA GCTGGCAATA AATGAGTTGA TAAGTGATGG CAGTGTGGTA
    TACGCCGAGG CTCTCTGGGA CCATGTCACT ATGGATGATG AAGAACTGGG TTTTAAGGCC
    GGAAGTGTCA TCGAAGTAAT GGATGCTACC AATAAAGAGT GGTGGTGGGG GAGGATACTT
    GATAGTGAAG GATGGTTTCC AGCCAGCTTT GTTAGGCTCC GTGTCAACCA GGATGAACCA
    ATGGAAGACT GTGCTACTAA AGCAGGGGAC AAAGAGCAGG ATTCTAGAAA TGTCCCAAGG
    AGAAACGGTT TTGGGCAGAA CAACAAAGAT CAAATGCGAA CGAATGTTAT TAATGAGATC
    TTAAATACAG AAAAACATTA TATTAAGCAC CTGAAGGATA TTTGTGAGGG CTACATCAAG
    CAGTGCCGTA AAAGGGACGA CATGTTTACA GAAGACCAAC TATGGACAAT CTTTGGAAAC
    ATTGAAGAGA TCTACAAATT CCAAAAGAAA TTCTTGAAAA CACTTGAAAA AAGAATTAAC
    AAAGATTCCC CGCATTTGAG TGAAATCGGC TCTTGCTTTT TGGAATATCA AACAGATTTC
    CAGATCTACT CAGAATATTG CAACAATCAT CCTAATGCCT GCACAGAACT TTCCAAACTC
    ACCAAGGTCA AGAAGTATGT CCATTTCTTT GAGACTTGCC GTTTATTACA GAAGATGATT
    GATATCTCTC TAGATGGGTT TCTTCTAACC CCGGTACAGA AGATCTGTAA ATATCCACTT
    CAGCTGGCGG AACTACTAAA GTACACTAAT TCCCAACACA GAGACTACAC AAACGTTGAG
    GCAGCCTTAG ATGCAATGAA AAACGTTGCC AGACTTATCA ATGAAAGAAA GCGGAGACTT
    GAGAACATTG ATAAAATAGC CCACTGGCAA AGTTCTATAG AGGACTGGGA GGGAGAAGAT
    ATCCTTGCCA GAAGCTCGGA GCTCATACAT TCTGGGGAAC TAACCAAGAT TTCTCACCTC
    CAGCCAAAGG GACAGCAGCG GATATTCTTT CTGTTTGATC ATCAAATTGT TTTCTGCAAG
    AAGGATCTGT TGCGACGTGA CATTCTGTAT TATAAAGGGA AGATCGACAT GGACGATATG
    GAGGTTCTGA ATGTAGAGGA TGGGAAAGAC AAAGACTTTA ACATTACTGT AAAAAATGCA
    TTCAAGCTGC AAGGCAAAGT CTCGGAAGAG ATTCACCTTT TCCTTGCAAA GAAACCAGAG
    CACAAACAGC GATGGCTACA GGCCTTTGAG GAAGAAAGGA AACAGGTGCT ATTGGATAAA
    GAGACAGGTT TCAGCATCAG TGAAATACAG AAGAAGCAGG CTATGATTAA TGCCAGTAAG
    CCTCACCCGG CAGGAAAGCC TAAAGGTGTC AATAGGACAT ACTATGATTT CTGGATGAAA
    CAAAAGCACC CCACTCTGCC TGCCAATCTC CCACAGCAAC AAGTCTTCAT GCTTGCCGAA
    CCCAAGCGCA AACCCTCTAA TTTCTGGCAG AATATCAGCC GACTGACCCC GTTCAGAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826421.1
    CDS42..2024
    Translation

    Target ORF information:

    RefSeq Version XM_012970967.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970967.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGTCAG GTTACTTATC ACTGGGAAGG ACAGGCAGCT CAGCACTGAG CCAGCACTGC 
    CAGGTACTTG TGCCTGCAAC AAGCAACAAG AGGCTTCTCT CAGCTGAGCT GTGCACCCAG
    TCTGCCCGGA TGAGGACTCG CCAATGCCCC AGTGATGCCA TGAACCCACA CAGCAGCAGT
    GGGAGTTCAG GGCTTTCCTC TGGCTCAGAT TCTGACAGCA GTAGCTGCCT TGGCTGGGGT
    GGTGCTGCTG CTGCTCCTGC TGCTGCTTCT GGGAGAGGAT TGCTGTCCAG ATACTGGAGC
    TTGGAAAGCC TTCACTCTGC TACCGTATTC AATGATGGGT CTACGAACAG ACCTTCGCTG
    TTTGATGACT TGGGGAGTGA TGAGGATTTT TATGAAGAAC TACACGTTTC TGGTCACCGC
    TTTGTTGGAG GGGGCGAGCA GCTGGCAATA AATGAGTTGA TAAGTGATGG CAGTGTGGTA
    TACGCCGAGG CTCTCTGGGA CCATGTCACT ATGGATGATG AAGAACTGGG TTTTAAGGCC
    GGAAGTGTCA TCGAAGTAAT GGATGCTACC AATAAAGAGT GGTGGTGGGG GAGGATACTT
    GATAGTGAAG GATGGTTTCC AGCCAGCTTT GTTAGGCTCC GTGTCAACCA GGATGAACCA
    ATGGAAGACT GTGCTACTAA AGCAGGGGAC AAAGAGCAGG ATTCTAGAAA TGTCCCAAGG
    AGAAACGGTT TTGGGCAGAA CAACAAAGAT CAAATGCGAA CGAATGTTAT TAATGAGATC
    TTAAATACAG AAAAACATTA TATTAAGCAC CTGAAGGATA TTTGTGAGGG CTACATCAAG
    CAGTGCCGTA AAAGGGACGA CATGTTTACA GAAGACCAAC TATGGACAAT CTTTGGAAAC
    ATTGAAGAGA TCTACAAATT CCAAAAGAAA TTCTTGAAAA CACTTGAAAA AAGAATTAAC
    AAAGATTCCC CGCATTTGAG TGAAATCGGC TCTTGCTTTT TGGAATATCA AACAGATTTC
    CAGATCTACT CAGAATATTG CAACAATCAT CCTAATGCCT GCACAGAACT TTCCAAACTC
    ACCAAGGTCA AGAAGTATGT CCATTTCTTT GAGACTTGCC GTTTATTACA GAAGATGATT
    GATATCTCTC TAGATGGGTT TCTTCTAACC CCGGTACAGA AGATCTGTAA ATATCCACTT
    CAGCTGGCGG AACTACTAAA GTACACTAAT TCCCAACACA GAGACTACAC AAACGTTGAG
    GCAGCCTTAG ATGCAATGAA AAACGTTGCC AGACTTATCA ATGAAAGAAA GCGGAGACTT
    GAGAACATTG ATAAAATAGC CCACTGGCAA AGTTCTATAG AGGACTGGGA GGGAGAAGAT
    ATCCTTGCCA GAAGCTCGGA GCTCATACAT TCTGGGGAAC TAACCAAGAT TTCTCACCTC
    CAGCCAAAGG GACAGCAGCG GATATTCTTT CTGTTTGATC ATCAAATTGT TTTCTGCAAG
    AAGGATCTGT TGCGACGTGA CATTCTGTAT TATAAAGGGA AGATCGACAT GGACGATATG
    GAGGTTCTGA ATGTAGAGGA TGGGAAAGAC AAAGACTTTA ACATTACTGT AAAAAATGCA
    TTCAAGCTGC AAGGCAAAGT CTCGGAAGAG ATTCACCTTT TCCTTGCAAA GAAACCAGAG
    CACAAACAGC GATGGCTACA GGCCTTTGAG GAAGAAAGGA AACAGGTGCT ATTGGATAAA
    GAGACAGGTT TCAGCATCAG TGAAATACAG AAGAAGCAGG CTATGATTAA TGCCAGTAAG
    CCTCACCCGG CAGGAAAGCC TAAAGGTGTC AATAGGACAT ACTATGATTT CTGGATGAAA
    CAAAAGCACC CCACTCTGCC TGCCAATCTC CCACAGCAAC AAGTCTTCAT GCTTGCCGAA
    CCCAAGCGCA AACCCTCTAA TTTCTGGCAG AATATCAGCC GACTGACCCC GTTCAGAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33789
    Clone ID Related Accession (Same CDS sequence) XM_012970967.2 , XM_012970967.3
    Accession Version XM_012970967.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970967.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGTCAG GTTACTTATC ACTGGGAAGG ACAGGCAGCT CAGCACTGAG CCAGCACTGC 
    CAGGTACTTG TGCCTGCAAC AAGCAACAAG AGGCTTCTCT CAGCTGAGCT GTGCACCCAG
    TCTGCCCGGA TGAGGACTCG CCAATGCCCC AGTGATGCCA TGAACCCACA CAGCAGCAGT
    GGGAGTTCAG GGCTTTCCTC TGGCTCAGAT TCTGACAGCA GTAGCTGCCT TGGCTGGGGT
    GGTGCTGCTG CTGCTCCTGC TGCTGCTTCT GGGAGAGGAT TGCTGTCCAG ATACTGGAGC
    TTGGAAAGCC TTCACTCTGC TACCGTATTC AATGATGGGT CTACGAACAG ACCTTCGCTG
    TTTGATGACT TGGGGAGTGA TGAGGATTTT TATGAAGAAC TACACGTTTC TGGTCACCGC
    TTTGTTGGAG GGGGCGAGCA GCTGGCAATA AATGAGTTGA TAAGTGATGG CAGTGTGGTA
    TACGCCGAGG CTCTCTGGGA CCATGTCACT ATGGATGATG AAGAACTGGG TTTTAAGGCC
    GGAAGTGTCA TCGAAGTAAT GGATGCTACC AATAAAGAGT GGTGGTGGGG GAGGATACTT
    GATAGTGAAG GATGGTTTCC AGCCAGCTTT GTTAGGCTCC GTGTCAACCA GGATGAACCA
    ATGGAAGACT GTGCTACTAA AGCAGGGGAC AAAGAGCAGG ATTCTAGAAA TGTCCCAAGG
    AGAAACGGTT TTGGGCAGAA CAACAAAGAT CAAATGCGAA CGAATGTTAT TAATGAGATC
    TTAAATACAG AAAAACATTA TATTAAGCAC CTGAAGGATA TTTGTGAGGG CTACATCAAG
    CAGTGCCGTA AAAGGGACGA CATGTTTACA GAAGACCAAC TATGGACAAT CTTTGGAAAC
    ATTGAAGAGA TCTACAAATT CCAAAAGAAA TTCTTGAAAA CACTTGAAAA AAGAATTAAC
    AAAGATTCCC CGCATTTGAG TGAAATCGGC TCTTGCTTTT TGGAATATCA AACAGATTTC
    CAGATCTACT CAGAATATTG CAACAATCAT CCTAATGCCT GCACAGAACT TTCCAAACTC
    ACCAAGGTCA AGAAGTATGT CCATTTCTTT GAGACTTGCC GTTTATTACA GAAGATGATT
    GATATCTCTC TAGATGGGTT TCTTCTAACC CCGGTACAGA AGATCTGTAA ATATCCACTT
    CAGCTGGCGG AACTACTAAA GTACACTAAT TCCCAACACA GAGACTACAC AAACGTTGAG
    GCAGCCTTAG ATGCAATGAA AAACGTTGCC AGACTTATCA ATGAAAGAAA GCGGAGACTT
    GAGAACATTG ATAAAATAGC CCACTGGCAA AGTTCTATAG AGGACTGGGA GGGAGAAGAT
    ATCCTTGCCA GAAGCTCGGA GCTCATACAT TCTGGGGAAC TAACCAAGAT TTCTCACCTC
    CAGCCAAAGG GACAGCAGCG GATATTCTTT CTGTTTGATC ATCAAATTGT TTTCTGCAAG
    AAGGATCTGT TGCGACGTGA CATTCTGTAT TATAAAGGGA AGATCGACAT GGACGATATG
    GAGGTTCTGA ATGTAGAGGA TGGGAAAGAC AAAGACTTTA ACATTACTGT AAAAAATGCA
    TTCAAGCTGC AAGGCAAAGT CTCGGAAGAG ATTCACCTTT TCCTTGCAAA GAAACCAGAG
    CACAAACAGC GATGGCTACA GGCCTTTGAG GAAGAAAGGA AACAGGTGCT ATTGGATAAA
    GAGACAGGTT TCAGCATCAG TGAAATACAG AAGAAGCAGG CTATGATTAA TGCCAGTAAG
    CCTCACCCGG CAGGAAAGCC TAAAGGTGTC AATAGGACAT ACTATGATTT CTGGATGAAA
    CAAAAGCACC CCACTCTGCC TGCCAATCTC CCACAGCAAC AAGTCTTCAT GCTTGCCGAA
    CCCAAGCGCA AACCCTCTAA TTTCTGGCAG AATATCAGCC GACTGACCCC GTTCAGAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826421.1
    CDS174..2156
    Translation

    Target ORF information:

    RefSeq Version XM_012970967.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970967.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGTCAG GTTACTTATC ACTGGGAAGG ACAGGCAGCT CAGCACTGAG CCAGCACTGC 
    CAGGTACTTG TGCCTGCAAC AAGCAACAAG AGGCTTCTCT CAGCTGAGCT GTGCACCCAG
    TCTGCCCGGA TGAGGACTCG CCAATGCCCC AGTGATGCCA TGAACCCACA CAGCAGCAGT
    GGGAGTTCAG GGCTTTCCTC TGGCTCAGAT TCTGACAGCA GTAGCTGCCT TGGCTGGGGT
    GGTGCTGCTG CTGCTCCTGC TGCTGCTTCT GGGAGAGGAT TGCTGTCCAG ATACTGGAGC
    TTGGAAAGCC TTCACTCTGC TACCGTATTC AATGATGGGT CTACGAACAG ACCTTCGCTG
    TTTGATGACT TGGGGAGTGA TGAGGATTTT TATGAAGAAC TACACGTTTC TGGTCACCGC
    TTTGTTGGAG GGGGCGAGCA GCTGGCAATA AATGAGTTGA TAAGTGATGG CAGTGTGGTA
    TACGCCGAGG CTCTCTGGGA CCATGTCACT ATGGATGATG AAGAACTGGG TTTTAAGGCC
    GGAAGTGTCA TCGAAGTAAT GGATGCTACC AATAAAGAGT GGTGGTGGGG GAGGATACTT
    GATAGTGAAG GATGGTTTCC AGCCAGCTTT GTTAGGCTCC GTGTCAACCA GGATGAACCA
    ATGGAAGACT GTGCTACTAA AGCAGGGGAC AAAGAGCAGG ATTCTAGAAA TGTCCCAAGG
    AGAAACGGTT TTGGGCAGAA CAACAAAGAT CAAATGCGAA CGAATGTTAT TAATGAGATC
    TTAAATACAG AAAAACATTA TATTAAGCAC CTGAAGGATA TTTGTGAGGG CTACATCAAG
    CAGTGCCGTA AAAGGGACGA CATGTTTACA GAAGACCAAC TATGGACAAT CTTTGGAAAC
    ATTGAAGAGA TCTACAAATT CCAAAAGAAA TTCTTGAAAA CACTTGAAAA AAGAATTAAC
    AAAGATTCCC CGCATTTGAG TGAAATCGGC TCTTGCTTTT TGGAATATCA AACAGATTTC
    CAGATCTACT CAGAATATTG CAACAATCAT CCTAATGCCT GCACAGAACT TTCCAAACTC
    ACCAAGGTCA AGAAGTATGT CCATTTCTTT GAGACTTGCC GTTTATTACA GAAGATGATT
    GATATCTCTC TAGATGGGTT TCTTCTAACC CCGGTACAGA AGATCTGTAA ATATCCACTT
    CAGCTGGCGG AACTACTAAA GTACACTAAT TCCCAACACA GAGACTACAC AAACGTTGAG
    GCAGCCTTAG ATGCAATGAA AAACGTTGCC AGACTTATCA ATGAAAGAAA GCGGAGACTT
    GAGAACATTG ATAAAATAGC CCACTGGCAA AGTTCTATAG AGGACTGGGA GGGAGAAGAT
    ATCCTTGCCA GAAGCTCGGA GCTCATACAT TCTGGGGAAC TAACCAAGAT TTCTCACCTC
    CAGCCAAAGG GACAGCAGCG GATATTCTTT CTGTTTGATC ATCAAATTGT TTTCTGCAAG
    AAGGATCTGT TGCGACGTGA CATTCTGTAT TATAAAGGGA AGATCGACAT GGACGATATG
    GAGGTTCTGA ATGTAGAGGA TGGGAAAGAC AAAGACTTTA ACATTACTGT AAAAAATGCA
    TTCAAGCTGC AAGGCAAAGT CTCGGAAGAG ATTCACCTTT TCCTTGCAAA GAAACCAGAG
    CACAAACAGC GATGGCTACA GGCCTTTGAG GAAGAAAGGA AACAGGTGCT ATTGGATAAA
    GAGACAGGTT TCAGCATCAG TGAAATACAG AAGAAGCAGG CTATGATTAA TGCCAGTAAG
    CCTCACCCGG CAGGAAAGCC TAAAGGTGTC AATAGGACAT ACTATGATTT CTGGATGAAA
    CAAAAGCACC CCACTCTGCC TGCCAATCTC CCACAGCAAC AAGTCTTCAT GCTTGCCGAA
    CCCAAGCGCA AACCCTCTAA TTTCTGGCAG AATATCAGCC GACTGACCCC GTTCAGAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58893
    Clone ID Related Accession (Same CDS sequence) XM_031902570.1
    Accession Version XM_031902570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGAATGC AGTGGAAAGG AATGGCTACT TTAGAAGGAA ACAATCATTT CTCCAAGCAC 
    CAGGATAGTA GACAACTTGT CCCATCATCG ATACTAAAGA TGCAAAGGGA AAGGAATGCT
    TTGCTTTACA AGAAGAGACC TACAGACCTG ACGGCTTCTC AGTACCTGCC TATCAACACT
    GAAAGTAGAA CACTGACACT GCCCCAGATG GCTTCCATGG GACACAAAAG GGATTCAGAA
    CCAACAAGGC CTAAAGCTTT ATTCAAGAGC CTGAGTTGTG ATAACTTGTG GGTATATGAG
    AGGAACCAAG AGAGGAAACT AGACAAGTCT ATAGATGCAG GAGATCTCAT GAGGAACCAG
    TCAAAAGCTT CTATGAGTGT CTCTATTGGG CCTCCAGAAA TATTCCGAAG TTTCCCAATG
    AAGGTGCACT CGTTTTCGCG GAGCACACCG AGCAGGCTTG ATCTTGTGGG CTGTGTGAGG
    CGCACCTCTC TCTCAGTATT CAATGATGGG TCTACGAACA GACCTTCGCT GTTTGATGAC
    TTGGGGAGTG ATGAGGATTT TTATGAAGAA CTACACGTTT CTGGTCACCG CTTTGTTGGA
    GGGGGCGAGC AGCTGGCAAT AAATGAGTTG ATAAGTGATG GCAGTGTGGT ATACGCCGAG
    GCTCTCTGGG ACCATGTCAC TATGGATGAT GAAGAACTGG GTTTTAAGGC CGGAAGTGTC
    ATCGAAGTAA TGGATGCTAC CAATAAAGAG TGGTGGTGGG GGAGGATACT TGATAGTGAA
    GGATGGTTTC CAGCCAGCTT TGTTAGGCTC CGTGTCAACC AGGATGAACC AATGGAAGAC
    TGTGCTACTA AAGCAGGGGA CAAAGAGCAG GATTCTAGAA ATGTCCCAAG GAGAAACGGT
    TTTGGGCAGA ACAACAAAGA TCAAATGCGA ACGAATGTTA TTAATGAGAT CTTAAATACA
    GAAAAACATT ATATTAAGCA CCTGAAGGAT ATTTGTGAGG GCTACATCAA GCAGTGCCGT
    AAAAGGGACG ACATGTTTAC AGAAGACCAA CTATGGACAA TCTTTGGAAA CATTGAAGAG
    ATCTACAAAT TCCAAAAGAA ATTCTTGAAA ACACTTGAAA AAAGAATTAA CAAAGATTCC
    CCGCATTTGA GTGAAATCGG CTCTTGCTTT TTGGAATATC AAACAGATTT CCAGATCTAC
    TCAGAATATT GCAACAATCA TCCTAATGCC TGCACAGAAC TTTCCAAACT CACCAAGGTC
    AAGAAGTATG TCCATTTCTT TGAGACTTGC CGTTTATTAC AGAAGATGAT TGATATCTCT
    CTAGATGGGT TTCTTCTAAC CCCGGTACAG AAGATCTGTA AATATCCACT TCAGCTGGCG
    GAACTACTAA AGTACACTAA TTCCCAACAC AGAGACTACA CAAACGTTGA GGCAGCCTTA
    GATGCAATGA AAAACGTTGC CAGACTTATC AATGAAAGAA AGCGGAGACT TGAGAACATT
    GATAAAATAG CCCACTGGCA AAGTTCTATA GAGGACTGGG AGGGAGAAGA TATCCTTGCC
    AGAAGCTCGG AGCTCATACA TTCTGGGGAA CTAACCAAGA TTTCTCACCT CCAGCCAAAG
    GGACAGCAGC GGATATTCTT TCTGTTTGAT CATCAAATTG TTTTCTGCAA GAAGGATCTG
    TTGCGACGTG ACATTCTGTA TTATAAAGGG AAGATCGACA TGGACGATAT GGAGGTTCTG
    AATGTAGAGG ATGGGAAAGA CAAAGACTTT AACATTACTG TAAAAAATGC ATTCAAGCTG
    CAAGGCAAAG TCTCGGAAGA GATTCACCTT TTCCTTGCAA AGAAACCAGA GCACAAACAG
    CGATGGCTAC AGGCCTTTGA GGAAGAAAGG AAACAGGTGC TATTGGATAA AGAGACAGGT
    TTCAGCATCA GTGAAATACA GAAGAAGCAG GCTATGATTA ATGCCAGTAA GCCTCACCCG
    GCAGGAAAGC CTAAAGGTGT CAATAGGACA TACTATGATT TCTGGATGAA ACAAAAGCAC
    CCCACTCTGC CTGCCAATCT CCCACAGCAA CAAGTCTTCA TGCTTGCCGA ACCCAAGCGC
    AAACCCTCTA ATTTCTGGCA GAATATCAGC CGACTGACCC CGTTCAGAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758430.1
    CDS16..2169
    Translation

    Target ORF information:

    RefSeq Version XM_031902570.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGAATGC AGTGGAAAGG AATGGCTACT TTAGAAGGAA ACAATCATTT CTCCAAGCAC 
    CAGGATAGTA GACAACTTGT CCCATCATCG ATACTAAAGA TGCAAAGGGA AAGGAATGCT
    TTGCTTTACA AGAAGAGACC TACAGACCTG ACGGCTTCTC AGTACCTGCC TATCAACACT
    GAAAGTAGAA CACTGACACT GCCCCAGATG GCTTCCATGG GACACAAAAG GGATTCAGAA
    CCAACAAGGC CTAAAGCTTT ATTCAAGAGC CTGAGTTGTG ATAACTTGTG GGTATATGAG
    AGGAACCAAG AGAGGAAACT AGACAAGTCT ATAGATGCAG GAGATCTCAT GAGGAACCAG
    TCAAAAGCTT CTATGAGTGT CTCTATTGGG CCTCCAGAAA TATTCCGAAG TTTCCCAATG
    AAGGTGCACT CGTTTTCGCG GAGCACACCG AGCAGGCTTG ATCTTGTGGG CTGTGTGAGG
    CGCACCTCTC TCTCAGTATT CAATGATGGG TCTACGAACA GACCTTCGCT GTTTGATGAC
    TTGGGGAGTG ATGAGGATTT TTATGAAGAA CTACACGTTT CTGGTCACCG CTTTGTTGGA
    GGGGGCGAGC AGCTGGCAAT AAATGAGTTG ATAAGTGATG GCAGTGTGGT ATACGCCGAG
    GCTCTCTGGG ACCATGTCAC TATGGATGAT GAAGAACTGG GTTTTAAGGC CGGAAGTGTC
    ATCGAAGTAA TGGATGCTAC CAATAAAGAG TGGTGGTGGG GGAGGATACT TGATAGTGAA
    GGATGGTTTC CAGCCAGCTT TGTTAGGCTC CGTGTCAACC AGGATGAACC AATGGAAGAC
    TGTGCTACTA AAGCAGGGGA CAAAGAGCAG GATTCTAGAA ATGTCCCAAG GAGAAACGGT
    TTTGGGCAGA ACAACAAAGA TCAAATGCGA ACGAATGTTA TTAATGAGAT CTTAAATACA
    GAAAAACATT ATATTAAGCA CCTGAAGGAT ATTTGTGAGG GCTACATCAA GCAGTGCCGT
    AAAAGGGACG ACATGTTTAC AGAAGACCAA CTATGGACAA TCTTTGGAAA CATTGAAGAG
    ATCTACAAAT TCCAAAAGAA ATTCTTGAAA ACACTTGAAA AAAGAATTAA CAAAGATTCC
    CCGCATTTGA GTGAAATCGG CTCTTGCTTT TTGGAATATC AAACAGATTT CCAGATCTAC
    TCAGAATATT GCAACAATCA TCCTAATGCC TGCACAGAAC TTTCCAAACT CACCAAGGTC
    AAGAAGTATG TCCATTTCTT TGAGACTTGC CGTTTATTAC AGAAGATGAT TGATATCTCT
    CTAGATGGGT TTCTTCTAAC CCCGGTACAG AAGATCTGTA AATATCCACT TCAGCTGGCG
    GAACTACTAA AGTACACTAA TTCCCAACAC AGAGACTACA CAAACGTTGA GGCAGCCTTA
    GATGCAATGA AAAACGTTGC CAGACTTATC AATGAAAGAA AGCGGAGACT TGAGAACATT
    GATAAAATAG CCCACTGGCA AAGTTCTATA GAGGACTGGG AGGGAGAAGA TATCCTTGCC
    AGAAGCTCGG AGCTCATACA TTCTGGGGAA CTAACCAAGA TTTCTCACCT CCAGCCAAAG
    GGACAGCAGC GGATATTCTT TCTGTTTGAT CATCAAATTG TTTTCTGCAA GAAGGATCTG
    TTGCGACGTG ACATTCTGTA TTATAAAGGG AAGATCGACA TGGACGATAT GGAGGTTCTG
    AATGTAGAGG ATGGGAAAGA CAAAGACTTT AACATTACTG TAAAAAATGC ATTCAAGCTG
    CAAGGCAAAG TCTCGGAAGA GATTCACCTT TTCCTTGCAA AGAAACCAGA GCACAAACAG
    CGATGGCTAC AGGCCTTTGA GGAAGAAAGG AAACAGGTGC TATTGGATAA AGAGACAGGT
    TTCAGCATCA GTGAAATACA GAAGAAGCAG GCTATGATTA ATGCCAGTAA GCCTCACCCG
    GCAGGAAAGC CTAAAGGTGT CAATAGGACA TACTATGATT TCTGGATGAA ACAAAAGCAC
    CCCACTCTGC CTGCCAATCT CCCACAGCAA CAAGTCTTCA TGCTTGCCGA ACCCAAGCGC
    AAACCCTCTA ATTTCTGGCA GAATATCAGC CGACTGACCC CGTTCAGAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58891
    Clone ID Related Accession (Same CDS sequence) XM_004917799.4
    Accession Version XM_004917799.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917799.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
    TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG
    GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC
    TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT
    AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA
    CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT
    CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC
    CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA
    CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT
    ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA
    TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA
    TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC
    CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT
    GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA
    GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA
    CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT
    TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA
    AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG
    GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA
    TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA
    AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA
    GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC
    CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA
    TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC
    AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA
    AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT
    GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA
    CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT
    CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG
    GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT
    GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT
    GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG
    AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG
    GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA
    TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA
    GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG
    GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT
    CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA
    ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG
    AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT
    GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA
    CCCCCCACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG
    GAAAGGTTGA TGTGGGCGCA TAAATCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT
    AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG
    CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT
    AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG
    GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC
    TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA
    CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA
    ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA
    AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC
    ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG
    GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA
    GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA
    GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC
    ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGATA GTAGACAACT TGTCCCATCA
    TCGATACTAA AGATGCAAAG GGAAAGGAAT GCTTTGCTTT ACAAGAAGAG ACCTACAGAC
    CTGACGGCTT CTCAGTACCT GCCTATCAAC ACTGAAAGTA GAACACTGAC ACTGCCCCAG
    ATGGCTTCCA TGGGACACAA AAGGGATTCA GAACCAACAA GGCCTAAAGC TTTATTCAAG
    AGCCTGAGTT GTGATAACTT GTGGGTATAT GAGAGGAACC AAGAGAGGAA ACTAGACAAG
    TCTATAGATG CAGGAGATCT CATGAGGAAC CAGTCAAAAG CTTCTATGAG TGTCTCTATT
    GGGCCTCCAG AAATATTCCG AAGTTTCCCA ATGAAGGTGC ACTCGTTTTC GCGGAGCACA
    CCGAGCAGGC TTGATCTTGT GGGCTGTGTG AGGCGCACCT CTCTCTCAGT ATTCAATGAT
    GGGTCTACGA ACAGACCTTC GCTGTTTGAT GACTTGGGGA GTGATGAGGA TTTTTATGAA
    GAACTACACG TTTCTGGTCA CCGCTTTGTT GGAGGGGGCG AGCAGCTGGC AATAAATGAG
    TTGATAAGTG ATGGCAGTGT GGTATACGCC GAGGCTCTCT GGGACCATGT CACTATGGAT
    GATGAAGAAC TGGGTTTTAA GGCCGGAAGT GTCATCGAAG TAATGGATGC TACCAATAAA
    GAGTGGTGGT GGGGGAGGAT ACTTGATAGT GAAGGATGGT TTCCAGCCAG CTTTGTTAGG
    CTCCGTGTCA ACCAGGATGA ACCAATGGAA GACTGTGCTA CTAAAGCAGG GGACAAAGAG
    CAGGATTCTA GAAATGTCCC AAGGAGAAAC GGTTTTGGGC AGAACAACAA AGATCAAATG
    CGAACGAATG TTATTAATGA GATCTTAAAT ACAGAAAAAC ATTATATTAA GCACCTGAAG
    GATATTTGTG AGGGCTACAT CAAGCAGTGC CGTAAAAGGG ACGACATGTT TACAGAAGAC
    CAACTATGGA CAATCTTTGG AAACATTGAA GAGATCTACA AATTCCAAAA GAAATTCTTG
    AAAACACTTG AAAAAAGAAT TAACAAAGAT TCCCCGCATT TGAGTGAAAT CGGCTCTTGC
    TTTTTGGAAT ATCAAACAGA TTTCCAGATC TACTCAGAAT ATTGCAACAA TCATCCTAAT
    GCCTGCACAG AACTTTCCAA ACTCACCAAG GTCAAGAAGT ATGTCCATTT CTTTGAGACT
    TGCCGTTTAT TACAGAAGAT GATTGATATC TCTCTAGATG GGTTTCTTCT AACCCCGGTA
    CAGAAGATCT GTAAATATCC ACTTCAGCTG GCGGAACTAC TAAAGTACAC TAATTCCCAA
    CACAGAGACT ACACAAACGT TGAGGCAGCC TTAGATGCAA TGAAAAACGT TGCCAGACTT
    ATCAATGAAA GAAAGCGGAG ACTTGAGAAC ATTGATAAAA TAGCCCACTG GCAAAGTTCT
    ATAGAGGACT GGGAGGGAGA AGATATCCTT GCCAGAAGCT CGGAGCTCAT ACATTCTGGG
    GAACTAACCA AGATTTCTCA CCTCCAGCCA AAGGGACAGC AGCGGATATT CTTTCTGTTT
    GATCATCAAA TTGTTTTCTG CAAGAAGGAT CTGTTGCGAC GTGACATTCT GTATTATAAA
    GGGAAGATCG ACATGGACGA TATGGAGGTT CTGAATGTAG AGGATGGGAA AGACAAAGAC
    TTTAACATTA CTGTAAAAAA TGCATTCAAG CTGCAAGGCA AAGTCTCGGA AGAGATTCAC
    CTTTTCCTTG CAAAGAAACC AGAGCACAAA CAGCGATGGC TACAGGCCTT TGAGGAAGAA
    AGGAAACAGG TGCTATTGGA TAAAGAGACA GGTTTCAGCA TCAGTGAAAT ACAGAAGAAG
    CAGGCTATGA TTAATGCCAG TAAGCCTCAC CCGGCAGGAA AGCCTAAAGG TGTCAATAGG
    ACATACTATG ATTTCTGGAT GAAACAAAAG CACCCCACTC TGCCTGCCAA TCTCCCACAG
    CAACAAGTCT TCATGCTTGC CGAACCCAAG CGCAAACCCT CTAATTTCTG GCAGAATATC
    AGCCGACTGA CCCCGTTCAG AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917856.1
    CDS178..5604
    Translation

    Target ORF information:

    RefSeq Version XM_004917799.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917799.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
    TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG
    GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC
    TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT
    AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA
    CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT
    CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC
    CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA
    CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT
    ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA
    TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA
    TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC
    CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT
    GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA
    GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA
    CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT
    TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA
    AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG
    GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA
    TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA
    AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA
    GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC
    CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA
    TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC
    AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA
    AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT
    GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA
    CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT
    CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG
    GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT
    GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT
    GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG
    AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG
    GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA
    TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA
    GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG
    GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT
    CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA
    ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG
    AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT
    GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA
    CCCCCCACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG
    GAAAGGTTGA TGTGGGCGCA TAAATCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT
    AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG
    CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT
    AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG
    GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC
    TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA
    CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA
    ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA
    AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC
    ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG
    GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA
    GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA
    GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC
    ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGATA GTAGACAACT TGTCCCATCA
    TCGATACTAA AGATGCAAAG GGAAAGGAAT GCTTTGCTTT ACAAGAAGAG ACCTACAGAC
    CTGACGGCTT CTCAGTACCT GCCTATCAAC ACTGAAAGTA GAACACTGAC ACTGCCCCAG
    ATGGCTTCCA TGGGACACAA AAGGGATTCA GAACCAACAA GGCCTAAAGC TTTATTCAAG
    AGCCTGAGTT GTGATAACTT GTGGGTATAT GAGAGGAACC AAGAGAGGAA ACTAGACAAG
    TCTATAGATG CAGGAGATCT CATGAGGAAC CAGTCAAAAG CTTCTATGAG TGTCTCTATT
    GGGCCTCCAG AAATATTCCG AAGTTTCCCA ATGAAGGTGC ACTCGTTTTC GCGGAGCACA
    CCGAGCAGGC TTGATCTTGT GGGCTGTGTG AGGCGCACCT CTCTCTCAGT ATTCAATGAT
    GGGTCTACGA ACAGACCTTC GCTGTTTGAT GACTTGGGGA GTGATGAGGA TTTTTATGAA
    GAACTACACG TTTCTGGTCA CCGCTTTGTT GGAGGGGGCG AGCAGCTGGC AATAAATGAG
    TTGATAAGTG ATGGCAGTGT GGTATACGCC GAGGCTCTCT GGGACCATGT CACTATGGAT
    GATGAAGAAC TGGGTTTTAA GGCCGGAAGT GTCATCGAAG TAATGGATGC TACCAATAAA
    GAGTGGTGGT GGGGGAGGAT ACTTGATAGT GAAGGATGGT TTCCAGCCAG CTTTGTTAGG
    CTCCGTGTCA ACCAGGATGA ACCAATGGAA GACTGTGCTA CTAAAGCAGG GGACAAAGAG
    CAGGATTCTA GAAATGTCCC AAGGAGAAAC GGTTTTGGGC AGAACAACAA AGATCAAATG
    CGAACGAATG TTATTAATGA GATCTTAAAT ACAGAAAAAC ATTATATTAA GCACCTGAAG
    GATATTTGTG AGGGCTACAT CAAGCAGTGC CGTAAAAGGG ACGACATGTT TACAGAAGAC
    CAACTATGGA CAATCTTTGG AAACATTGAA GAGATCTACA AATTCCAAAA GAAATTCTTG
    AAAACACTTG AAAAAAGAAT TAACAAAGAT TCCCCGCATT TGAGTGAAAT CGGCTCTTGC
    TTTTTGGAAT ATCAAACAGA TTTCCAGATC TACTCAGAAT ATTGCAACAA TCATCCTAAT
    GCCTGCACAG AACTTTCCAA ACTCACCAAG GTCAAGAAGT ATGTCCATTT CTTTGAGACT
    TGCCGTTTAT TACAGAAGAT GATTGATATC TCTCTAGATG GGTTTCTTCT AACCCCGGTA
    CAGAAGATCT GTAAATATCC ACTTCAGCTG GCGGAACTAC TAAAGTACAC TAATTCCCAA
    CACAGAGACT ACACAAACGT TGAGGCAGCC TTAGATGCAA TGAAAAACGT TGCCAGACTT
    ATCAATGAAA GAAAGCGGAG ACTTGAGAAC ATTGATAAAA TAGCCCACTG GCAAAGTTCT
    ATAGAGGACT GGGAGGGAGA AGATATCCTT GCCAGAAGCT CGGAGCTCAT ACATTCTGGG
    GAACTAACCA AGATTTCTCA CCTCCAGCCA AAGGGACAGC AGCGGATATT CTTTCTGTTT
    GATCATCAAA TTGTTTTCTG CAAGAAGGAT CTGTTGCGAC GTGACATTCT GTATTATAAA
    GGGAAGATCG ACATGGACGA TATGGAGGTT CTGAATGTAG AGGATGGGAA AGACAAAGAC
    TTTAACATTA CTGTAAAAAA TGCATTCAAG CTGCAAGGCA AAGTCTCGGA AGAGATTCAC
    CTTTTCCTTG CAAAGAAACC AGAGCACAAA CAGCGATGGC TACAGGCCTT TGAGGAAGAA
    AGGAAACAGG TGCTATTGGA TAAAGAGACA GGTTTCAGCA TCAGTGAAAT ACAGAAGAAG
    CAGGCTATGA TTAATGCCAG TAAGCCTCAC CCGGCAGGAA AGCCTAAAGG TGTCAATAGG
    ACATACTATG ATTTCTGGAT GAAACAAAAG CACCCCACTC TGCCTGCCAA TCTCCCACAG
    CAACAAGTCT TCATGCTTGC CGAACCCAAG CGCAAACCCT CTAATTTCTG GCAGAATATC
    AGCCGACTGA CCCCGTTCAG AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58892
    Clone ID Related Accession (Same CDS sequence) XM_031902569.1
    Accession Version XM_031902569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
    TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG
    GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC
    TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT
    AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA
    CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT
    CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC
    CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA
    CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT
    ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA
    TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA
    TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC
    CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT
    GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA
    GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA
    CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT
    TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA
    AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG
    GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA
    TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA
    AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA
    GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC
    CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA
    TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC
    AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA
    AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT
    GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA
    CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT
    CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG
    GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT
    GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT
    GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG
    AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG
    GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA
    TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA
    GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG
    GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT
    CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA
    ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG
    AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT
    GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA
    CCCCCCACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG
    GAAAGGTTGA TGTGGGCGCA TAAATCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT
    AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG
    CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT
    AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG
    GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC
    TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA
    CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA
    ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA
    AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC
    ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG
    GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA
    GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA
    GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC
    ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGCTT CTATGAGTGT CTCTATTGGG
    CCTCCAGAAA TATTCCGAAG TTTCCCAATG AAGGTGCACT CGTTTTCGCG GAGCACACCG
    AGCAGGCTTG ATCTTGTGGG CTGTGTGAGG CGCACCTCTC TCTCAGTATT CAATGATGGG
    TCTACGAACA GACCTTCGCT GTTTGATGAC TTGGGGAGTG ATGAGGATTT TTATGAAGAA
    CTACACGTTT CTGGTCACCG CTTTGTTGGA GGGGGCGAGC AGCTGGCAAT AAATGAGTTG
    ATAAGTGATG GCAGTGTGGT ATACGCCGAG GCTCTCTGGG ACCATGTCAC TATGGATGAT
    GAAGAACTGG GTTTTAAGGC CGGAAGTGTC ATCGAAGTAA TGGATGCTAC CAATAAAGAG
    TGGTGGTGGG GGAGGATACT TGATAGTGAA GGATGGTTTC CAGCCAGCTT TGTTAGGCTC
    CGTGTCAACC AGGATGAACC AATGGAAGAC TGTGCTACTA AAGCAGGGGA CAAAGAGCAG
    GATTCTAGAA ATGTCCCAAG GAGAAACGGT TTTGGGCAGA ACAACAAAGA TCAAATGCGA
    ACGAATGTTA TTAATGAGAT CTTAAATACA GAAAAACATT ATATTAAGCA CCTGAAGGAT
    ATTTGTGAGG GCTACATCAA GCAGTGCCGT AAAAGGGACG ACATGTTTAC AGAAGACCAA
    CTATGGACAA TCTTTGGAAA CATTGAAGAG ATCTACAAAT TCCAAAAGAA ATTCTTGAAA
    ACACTTGAAA AAAGAATTAA CAAAGATTCC CCGCATTTGA GTGAAATCGG CTCTTGCTTT
    TTGGAATATC AAACAGATTT CCAGATCTAC TCAGAATATT GCAACAATCA TCCTAATGCC
    TGCACAGAAC TTTCCAAACT CACCAAGGTC AAGAAGTATG TCCATTTCTT TGAGACTTGC
    CGTTTATTAC AGAAGATGAT TGATATCTCT CTAGATGGGT TTCTTCTAAC CCCGGTACAG
    AAGATCTGTA AATATCCACT TCAGCTGGCG GAACTACTAA AGTACACTAA TTCCCAACAC
    AGAGACTACA CAAACGTTGA GGCAGCCTTA GATGCAATGA AAAACGTTGC CAGACTTATC
    AATGAAAGAA AGCGGAGACT TGAGAACATT GATAAAATAG CCCACTGGCA AAGTTCTATA
    GAGGACTGGG AGGGAGAAGA TATCCTTGCC AGAAGCTCGG AGCTCATACA TTCTGGGGAA
    CTAACCAAGA TTTCTCACCT CCAGCCAAAG GGACAGCAGC GGATATTCTT TCTGTTTGAT
    CATCAAATTG TTTTCTGCAA GAAGGATCTG TTGCGACGTG ACATTCTGTA TTATAAAGGG
    AAGATCGACA TGGACGATAT GGAGGTTCTG AATGTAGAGG ATGGGAAAGA CAAAGACTTT
    AACATTACTG TAAAAAATGC ATTCAAGCTG CAAGGCAAAG TCTCGGAAGA GATTCACCTT
    TTCCTTGCAA AGAAACCAGA GCACAAACAG CGATGGCTAC AGGCCTTTGA GGAAGAAAGG
    AAACAGGTGC TATTGGATAA AGAGACAGGT TTCAGCATCA GTGAAATACA GAAGAAGCAG
    GCTATGATTA ATGCCAGTAA GCCTCACCCG GCAGGAAAGC CTAAAGGTGT CAATAGGACA
    TACTATGATT TCTGGATGAA ACAAAAGCAC CCCACTCTGC CTGCCAATCT CCCACAGCAA
    CAAGTCTTCA TGCTTGCCGA ACCCAAGCGC AAACCCTCTA ATTTCTGGCA GAATATCAGC
    CGACTGACCC CGTTCAGAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758429.1
    CDS178..5301
    Translation

    Target ORF information:

    RefSeq Version XM_031902569.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
    TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG
    GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC
    TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT
    AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA
    CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT
    CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC
    CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA
    CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT
    ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA
    TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA
    TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC
    CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT
    GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA
    GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA
    CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT
    TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA
    AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG
    GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA
    TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA
    AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA
    GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC
    CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA
    TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC
    AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA
    AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT
    GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA
    CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT
    CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG
    GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT
    GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT
    GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG
    AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG
    GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA
    TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA
    GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG
    GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT
    CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA
    ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG
    AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT
    GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA
    CCCCCCACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG
    GAAAGGTTGA TGTGGGCGCA TAAATCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT
    AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG
    CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT
    AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG
    GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC
    TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA
    CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA
    ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA
    AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC
    ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG
    GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA
    GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA
    GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC
    ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGCTT CTATGAGTGT CTCTATTGGG
    CCTCCAGAAA TATTCCGAAG TTTCCCAATG AAGGTGCACT CGTTTTCGCG GAGCACACCG
    AGCAGGCTTG ATCTTGTGGG CTGTGTGAGG CGCACCTCTC TCTCAGTATT CAATGATGGG
    TCTACGAACA GACCTTCGCT GTTTGATGAC TTGGGGAGTG ATGAGGATTT TTATGAAGAA
    CTACACGTTT CTGGTCACCG CTTTGTTGGA GGGGGCGAGC AGCTGGCAAT AAATGAGTTG
    ATAAGTGATG GCAGTGTGGT ATACGCCGAG GCTCTCTGGG ACCATGTCAC TATGGATGAT
    GAAGAACTGG GTTTTAAGGC CGGAAGTGTC ATCGAAGTAA TGGATGCTAC CAATAAAGAG
    TGGTGGTGGG GGAGGATACT TGATAGTGAA GGATGGTTTC CAGCCAGCTT TGTTAGGCTC
    CGTGTCAACC AGGATGAACC AATGGAAGAC TGTGCTACTA AAGCAGGGGA CAAAGAGCAG
    GATTCTAGAA ATGTCCCAAG GAGAAACGGT TTTGGGCAGA ACAACAAAGA TCAAATGCGA
    ACGAATGTTA TTAATGAGAT CTTAAATACA GAAAAACATT ATATTAAGCA CCTGAAGGAT
    ATTTGTGAGG GCTACATCAA GCAGTGCCGT AAAAGGGACG ACATGTTTAC AGAAGACCAA
    CTATGGACAA TCTTTGGAAA CATTGAAGAG ATCTACAAAT TCCAAAAGAA ATTCTTGAAA
    ACACTTGAAA AAAGAATTAA CAAAGATTCC CCGCATTTGA GTGAAATCGG CTCTTGCTTT
    TTGGAATATC AAACAGATTT CCAGATCTAC TCAGAATATT GCAACAATCA TCCTAATGCC
    TGCACAGAAC TTTCCAAACT CACCAAGGTC AAGAAGTATG TCCATTTCTT TGAGACTTGC
    CGTTTATTAC AGAAGATGAT TGATATCTCT CTAGATGGGT TTCTTCTAAC CCCGGTACAG
    AAGATCTGTA AATATCCACT TCAGCTGGCG GAACTACTAA AGTACACTAA TTCCCAACAC
    AGAGACTACA CAAACGTTGA GGCAGCCTTA GATGCAATGA AAAACGTTGC CAGACTTATC
    AATGAAAGAA AGCGGAGACT TGAGAACATT GATAAAATAG CCCACTGGCA AAGTTCTATA
    GAGGACTGGG AGGGAGAAGA TATCCTTGCC AGAAGCTCGG AGCTCATACA TTCTGGGGAA
    CTAACCAAGA TTTCTCACCT CCAGCCAAAG GGACAGCAGC GGATATTCTT TCTGTTTGAT
    CATCAAATTG TTTTCTGCAA GAAGGATCTG TTGCGACGTG ACATTCTGTA TTATAAAGGG
    AAGATCGACA TGGACGATAT GGAGGTTCTG AATGTAGAGG ATGGGAAAGA CAAAGACTTT
    AACATTACTG TAAAAAATGC ATTCAAGCTG CAAGGCAAAG TCTCGGAAGA GATTCACCTT
    TTCCTTGCAA AGAAACCAGA GCACAAACAG CGATGGCTAC AGGCCTTTGA GGAAGAAAGG
    AAACAGGTGC TATTGGATAA AGAGACAGGT TTCAGCATCA GTGAAATACA GAAGAAGCAG
    GCTATGATTA ATGCCAGTAA GCCTCACCCG GCAGGAAAGC CTAAAGGTGT CAATAGGACA
    TACTATGATT TCTGGATGAA ACAAAAGCAC CCCACTCTGC CTGCCAATCT CCCACAGCAA
    CAAGTCTTCA TGCTTGCCGA ACCCAAGCGC AAACCCTCTA ATTTCTGGCA GAATATCAGC
    CGACTGACCC CGTTCAGAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa16058
    Clone ID Related Accession (Same CDS sequence) XM_004917799.3
    Accession Version XM_004917799.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917799.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
    TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG
    GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC
    TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT
    AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA
    CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT
    CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC
    CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA
    CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT
    ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA
    TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA
    TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC
    CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT
    GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA
    GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA
    CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT
    TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA
    AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG
    GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA
    TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA
    AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA
    GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC
    CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA
    TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC
    AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA
    AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT
    GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA
    CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT
    CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG
    GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT
    GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT
    GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG
    AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG
    GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA
    TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA
    GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG
    GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT
    CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA
    ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG
    AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT
    GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA
    CCCCCAACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG
    GAAAGGTTGA TGTGGGCGCA TAAGTCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT
    AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG
    CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT
    AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG
    GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC
    TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA
    CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA
    ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA
    AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC
    ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG
    GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA
    GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA
    GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC
    ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGATA GTAGACAACT TGTCCCATCA
    TCGATACTAA AGATGCAAAG GGAAAGGAAT GCTTTGCTTT ACAAGAAGAG ACCTACAGAC
    CTGACGGCTT CTCAGTACCT GCCTATCAAC ACTGAAAGTA GAACACTGAC ACTGCCCCAG
    ATGGCTTCCA TGGGACACAA AAGGGATTCA GAACCAACAA GGCCTAAAGC TTTATTCAAG
    AGCCTGAGTT GTGATAACTT GTGGGTATAT GAGAGGAACC AAGAGAGGAA ACTAGACAAG
    TCTATAGATG CAGGAGATCT CATGAGGAAC CAGTCAAAAG CTTCTATGAG TGTCTCTATT
    GGGCCTCCAG AAATATTCCG AAGTTTCCCA ATGAAGGTGC ACTCGTTTTC GCGGAGCACA
    CCGAGCAGGC TTGATCTTGT GGGCTGTGTG AGGCGCACCT CTCTCTCAGT ATTCAATGAT
    GGGTCTACGA ACAGACCTTC GCTGTTTGAT GACTTGGGGA GTGATGAGGA TTTTTATGAA
    GAACTACACG TTTCTGGTCA CCGCTTTGTT GGAGGGGGCG AGCAGCTGGC AATAAATGAG
    TTGATAAGTG ATGGCAGTGT GGTATACGCC GAGGCTCTCT GGGACCATGT CACTATGGAT
    GATGAAGAAC TGGGTTTTAA GGCCGGAAGT GTCATCGAAG TAATGGATGC TACCAATAAA
    GAGTGGTGGT GGGGGAGGAT ACTTGATAGT GAAGGATGGT TTCCAGCCAG CTTTGTTAGG
    CTCCGTGTCA ACCAGGATGA ACCAATGGAA GACTGTGCTA CTAAAGCAGG GGACAAAGAG
    CAGGATTCTA GAAATGTCCC AAGGAGAAAC GGTTTTGGGC AGAACAACAA AGATCAAATG
    CGAACGAATG TTATTAATGA GATCTTAAAT ACAGAAAAAC ATTATATTAA GCACCTGAAG
    GATATTTGTG AGGGCTACAT CAAGCAGTGC CGTAAAAGGG ACGACATGTT TACAGAAGAC
    CAACTATGGA CAATCTTTGG AAACATTGAA GAGATCTACA AATTCCAAAA GAAATTCTTG
    AAAACACTTG AAAAAAGAAT TAACAAAGAT TCCCCGCATT TGAGTGAAAT CGGCTCTTGC
    TTTTTGGAAT ATCAAACAGA TTTCCAGATC TACTCAGAAT ATTGCAACAA TCATCCTAAT
    GCCTGCACAG AACTTTCCAA ACTCACCAAG GTCAAGAAGT ATGTCCATTT CTTTGAGACT
    TGCCGTTTAT TACAGAAGAT GATTGATATC TCTCTAGATG GGTTTCTTCT AACCCCGGTA
    CAGAAGATCT GTAAATATCC ACTTCAGCTG GCGGAACTAC TAAAGTACAC TAATTCCCAA
    CACAGAGACT ACACAAACGT TGAGGCAGCC TTAGATGCAA TGAAAAACGT TGCCAGACTT
    ATCAATGAAA GAAAGCGGAG ACTTGAGAAC ATTGATAAAA TAGCCCACTG GCAAAGTTCT
    ATAGAGGACT GGGAGGGAGA AGATATCCTT GCCAGAAGCT CGGAGCTCAT ACATTCTGGG
    GAACTAACCA AGATTTCTCA CCTCCAGCCA AAGGGACAGC AGCGGATATT CTTTCTGTTT
    GATCATCAAA TTGTTTTCTG CAAGAAGGAT CTGTTGCGAC GTGACATTCT GTATTATAAA
    GGGAAGATCG ACATGGACGA TATGGAGGTT CTGAATGTAG AGGATGGGAA AGACAAAGAC
    TTTAACATTA CTGTAAAAAA TGCATTCAAG CTGCAAGGCA AAGTCTCGGA AGAGATTCAC
    CTTTTCCTTG CAAAGAAACC AGAGCACAAA CAGCGATGGC TACAGGCCTT TGAGGAAGAA
    AGGAAACAGG TGCTATTGGA TAAAGAGACA GGTTTCAGCA TCAGTGAAAT ACAGAAGAAG
    CAGGCTATGA TTAATGCCAG TAAGCCTCAC CCGGCAGGAA AGCCTAAAGG TGTCAATAGG
    ACATACTATG ATTTCTGGAT GAAACAAAAG CACCCCACTC TGCCTGCCAA TCTCCCACAG
    CAACAAGTCT TCATGCTTGC CGAACCCAAG CGCAAACCCT CTAATTTCTG GCAGAATATC
    AGCCGACTGA CCCCGTTCAG AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917856.1
    CDS165..5591
    Translation

    Target ORF information:

    RefSeq Version XM_004917799.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917799.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
    TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG
    GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC
    TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT
    AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA
    CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT
    CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC
    CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA
    CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT
    ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA
    TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA
    TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC
    CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT
    GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA
    GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA
    CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT
    TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA
    AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG
    GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA
    TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA
    AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA
    GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC
    CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA
    TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC
    AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA
    AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT
    GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA
    CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT
    CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG
    GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT
    GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT
    GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG
    AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG
    GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA
    TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA
    GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG
    GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT
    CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA
    ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG
    AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT
    GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA
    CCCCCAACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG
    GAAAGGTTGA TGTGGGCGCA TAAGTCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT
    AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG
    CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT
    AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG
    GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC
    TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA
    CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA
    ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA
    AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC
    ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG
    GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA
    GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA
    GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC
    ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGATA GTAGACAACT TGTCCCATCA
    TCGATACTAA AGATGCAAAG GGAAAGGAAT GCTTTGCTTT ACAAGAAGAG ACCTACAGAC
    CTGACGGCTT CTCAGTACCT GCCTATCAAC ACTGAAAGTA GAACACTGAC ACTGCCCCAG
    ATGGCTTCCA TGGGACACAA AAGGGATTCA GAACCAACAA GGCCTAAAGC TTTATTCAAG
    AGCCTGAGTT GTGATAACTT GTGGGTATAT GAGAGGAACC AAGAGAGGAA ACTAGACAAG
    TCTATAGATG CAGGAGATCT CATGAGGAAC CAGTCAAAAG CTTCTATGAG TGTCTCTATT
    GGGCCTCCAG AAATATTCCG AAGTTTCCCA ATGAAGGTGC ACTCGTTTTC GCGGAGCACA
    CCGAGCAGGC TTGATCTTGT GGGCTGTGTG AGGCGCACCT CTCTCTCAGT ATTCAATGAT
    GGGTCTACGA ACAGACCTTC GCTGTTTGAT GACTTGGGGA GTGATGAGGA TTTTTATGAA
    GAACTACACG TTTCTGGTCA CCGCTTTGTT GGAGGGGGCG AGCAGCTGGC AATAAATGAG
    TTGATAAGTG ATGGCAGTGT GGTATACGCC GAGGCTCTCT GGGACCATGT CACTATGGAT
    GATGAAGAAC TGGGTTTTAA GGCCGGAAGT GTCATCGAAG TAATGGATGC TACCAATAAA
    GAGTGGTGGT GGGGGAGGAT ACTTGATAGT GAAGGATGGT TTCCAGCCAG CTTTGTTAGG
    CTCCGTGTCA ACCAGGATGA ACCAATGGAA GACTGTGCTA CTAAAGCAGG GGACAAAGAG
    CAGGATTCTA GAAATGTCCC AAGGAGAAAC GGTTTTGGGC AGAACAACAA AGATCAAATG
    CGAACGAATG TTATTAATGA GATCTTAAAT ACAGAAAAAC ATTATATTAA GCACCTGAAG
    GATATTTGTG AGGGCTACAT CAAGCAGTGC CGTAAAAGGG ACGACATGTT TACAGAAGAC
    CAACTATGGA CAATCTTTGG AAACATTGAA GAGATCTACA AATTCCAAAA GAAATTCTTG
    AAAACACTTG AAAAAAGAAT TAACAAAGAT TCCCCGCATT TGAGTGAAAT CGGCTCTTGC
    TTTTTGGAAT ATCAAACAGA TTTCCAGATC TACTCAGAAT ATTGCAACAA TCATCCTAAT
    GCCTGCACAG AACTTTCCAA ACTCACCAAG GTCAAGAAGT ATGTCCATTT CTTTGAGACT
    TGCCGTTTAT TACAGAAGAT GATTGATATC TCTCTAGATG GGTTTCTTCT AACCCCGGTA
    CAGAAGATCT GTAAATATCC ACTTCAGCTG GCGGAACTAC TAAAGTACAC TAATTCCCAA
    CACAGAGACT ACACAAACGT TGAGGCAGCC TTAGATGCAA TGAAAAACGT TGCCAGACTT
    ATCAATGAAA GAAAGCGGAG ACTTGAGAAC ATTGATAAAA TAGCCCACTG GCAAAGTTCT
    ATAGAGGACT GGGAGGGAGA AGATATCCTT GCCAGAAGCT CGGAGCTCAT ACATTCTGGG
    GAACTAACCA AGATTTCTCA CCTCCAGCCA AAGGGACAGC AGCGGATATT CTTTCTGTTT
    GATCATCAAA TTGTTTTCTG CAAGAAGGAT CTGTTGCGAC GTGACATTCT GTATTATAAA
    GGGAAGATCG ACATGGACGA TATGGAGGTT CTGAATGTAG AGGATGGGAA AGACAAAGAC
    TTTAACATTA CTGTAAAAAA TGCATTCAAG CTGCAAGGCA AAGTCTCGGA AGAGATTCAC
    CTTTTCCTTG CAAAGAAACC AGAGCACAAA CAGCGATGGC TACAGGCCTT TGAGGAAGAA
    AGGAAACAGG TGCTATTGGA TAAAGAGACA GGTTTCAGCA TCAGTGAAAT ACAGAAGAAG
    CAGGCTATGA TTAATGCCAG TAAGCCTCAC CCGGCAGGAA AGCCTAAAGG TGTCAATAGG
    ACATACTATG ATTTCTGGAT GAAACAAAAG CACCCCACTC TGCCTGCCAA TCTCCCACAG
    CAACAAGTCT TCATGCTTGC CGAACCCAAG CGCAAACCCT CTAATTTCTG GCAGAATATC
    AGCCGACTGA CCCCGTTCAG AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa16059
    Clone ID Related Accession (Same CDS sequence) XM_002933352.4
    Accession Version XM_002933352.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933352.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCAGAAA TGGACATGAA GAATTCAAAA GAAAAGGTGG GTGGTATTTT ATCTGATTCT 
    TTTCAAGACA TATTTAAAGG TTATTGGGAA GAGGACAGGC ACTCACGATC CAGTGAATCT
    GAAGAAAAAC AAAATATGAC ACTAGGCAGT ATTAAAAACA GCAGCCTGTT AAGGAAACAG
    GTTAGCAGAC AAGAGCCAAT GAGTACTGGC CATTTTGAAG ACTTTCAAGC TATTGGTGAC
    TCCTCAAGTA GCCTACAAGA CAGATCGCCT GTTATTGTAC TATCTGCCCA TCAGCCAAGA
    ACACAAACAG CATGGAAGGA AATTGAGCAG AATACATATA AACATGAAAA TGGCCATTCT
    TCACATTCAA ACAGCGATGA AAATTTAAGT GGTAGCAGAG AGTCTTTAAA CCAGGACCTA
    GTTTATAAAG CAAGATATTC AATGGGATGT GTGGAACAAG AAGAAGATGA GCAAAATGGC
    CCCACTTTCG TTGATATAAA CAGGGAAACT CCTCTCTTTC AAAAAGACTC AGAAACAATA
    TTTGTTCAAG AACATACAGA AGCCCATGGC AGGCACTTGT GGGACATATT GTTAGATAAA
    GAACAACACA ATCCCTCTCA AATAAGTATT CAAACAGCTA AAATAAAGTC CCTGAAGCAA
    AAAAGTTATA AGGATCCAAA CTACCATAGT AATGGTAATA TGGAGGATTC TAATCTTGAC
    CATGGTGACA GTGTCTTCTC TGCACAAAGA CGATATCTCA CAGCCCCACA AGATTCTGAT
    GGAGATGAAA GTGACTTTGA AGTTTCATCC TCGGAAGAAA ATAGAAATCA TTGTCTGGCA
    ACAGTTGAGG AGAGCAGAGA CGGCATGTGT TATTACAGTA CTATAAAGAA AATAGACACT
    ATAGACACAG TGGATGATAA TGAAAGCAAC TGCAGCAGCT CTAACAGTGA AGGCACTGAA
    GGCAACATGG GATACAACTG GAAAAGTGAG ATCAACAAAA TAGAAGACAT TAAAAGTGAC
    GTAAAGGCAT CAGAAAGCCA AAAAGACTCC AAAATAGCAG AATTAAAAGA TTCCAGAACA
    GAAATAAAAG AGGAGCCTAA CAATAAAATA AAACGGATGA ATATAAAAAA TAGCGACATT
    TTTAAAAAAT ATCTGTCGGT TAAAACACCT GTGCCAAATG GCTCACAACT CGCTGCTAAA
    CCTACAGGGG AAACGTCGGA CTTCTCTGAT GAGGATTCAT ATGAAAGTAG CTTTCTATAC
    AACAGGAGTA TGAGGAAGGC ATCAGGGTCC GGGAGAATTG ATCTAGATAA TTTTACCAGA
    TTAAGATGTG GTGTTGCGCC ATCAAATACC AATGATAAGT CAGACAATGG GCAAAGTCAG
    GGGTTCTCCA AACCCCCAAC AGAAAACTGT GAAACGAAGA GTGAAAGAAA CACCGAGAGT
    AAACGGTTAA AGGAAAGGTT GATGTGGGCG CATAAGTCTT TCTCTAGTAT GTTTGATTTT
    AAGAATAATG ATAAAGATAG TGCCGCCCAG AGTTCAGAGG GCACCCCTAG AGAGGAGAAG
    AAAAAGTCTA GGCCACTTCA GTCATCCTGG AAAACACTCC GAAATTCCAA AAACAAAGAT
    AAAGACTCAT TTAAGAGGCT CAGCGTGTTG AATTTATCAG CACATATTAT AAATACAAAC
    ATGCCAAGGG GGGCTTCAAA GGAAAATGGC AATGAACATC TACCCCCGAT TCTTGATCCA
    ATAAAACAAC CCTCCTCTAT GGAAATAAAG CCTTGTATGA CTACTGATAC AAACACAGAG
    TCAGATTTGG AACCTCTGGA AGCCAAGGAA AACCATAAAA AATGGAGCCT TGAAAATGAT
    GATTCTTTAC AAACTACTTG TGATGACTCA GCTTTTTTTT CACAATTTGA CTCAAACGAA
    TCACATTCGT TAAACAAACA GCCCTCTATT ACAACTTTAT TTAACCCATT CTCAAATTCT
    GAAATAAATC CCATGCCATG TCGACCAATG AGTCCAAAGC CTTTTAGCCA ATGGCCCAGC
    TTTCAGCGTA GGGGCTTGCA CAATTCCAGG ATAAGTGCTA CTTCGATGAC CTCTCTAGCA
    AACAGTTCTA CAGTGGATGG GTGCCCAGAT TCCCCAGAGA CAGCAGTAAA AACATGGGGA
    GGGTATTTAA CAGACAGAAG TAGTCAGAAG GATGACAGCA GTGCCAGCAG CCAATCAAAG
    CCCAGCCTTA ACACTGCTTC TTCTGTAATG GACATACTAA GAGATGAGGA TAGTAGACAA
    CTTGTCCCAT CATCGATACT AAAGATGCAA AGGGAAAGGA ATGCTTTGCT TTACAAGAAG
    AGACCTACAG ACCTGACGGC TTCTCAGTAC CTGCCTATCA ACACTGAAAG TAGAACACTG
    ACACTGCCCC AGATGGCTTC CATGGGACAC AAAAGGGATT CAGAACCAAC AAGGCCTAAA
    GCTTTATTCA AGAGCCTGAG TTGTGATAAC TTGTGGGTAT ATGAGAGGAA CCAAGAGAGG
    AAACTAGACA AGTCTATAGA TGCAGGAGAT CTCATGAGGA ACCAGTCAAA AGCTTCTATG
    AGTGTCTCTA TTGGGCCTCC AGAAATATTC CGAAGTTTCC CAATGAAGGT GCACTCGTTT
    TCGCGGAGCA CACCGAGCAG GCTTGATCTT GTGGGCTGTG TGAGGCGCAC CTCTCTCTCA
    GTATTCAATG ATGGGTCTAC GAACAGACCT TCGCTGTTTG ATGACTTGGG GAGTGATGAG
    GATTTTTATG AAGAACTACA CGTTTCTGGT CACCGCTTTG TTGGAGGGGG CGAGCAGCTG
    GCAATAAATG AGTTGATAAG TGATGGCAGT GTGGTATACG CCGAGGCTCT CTGGGACCAT
    GTCACTATGG ATGATGAAGA ACTGGGTTTT AAGGCCGGAA GTGTCATCGA AGTAATGGAT
    GCTACCAATA AAGAGTGGTG GTGGGGGAGG ATACTTGATA GTGAAGGATG GTTTCCAGCC
    AGCTTTGTTA GGCTCCGTGT CAACCAGGAT GAACCAATGG AAGACTGTGC TACTAAAGCA
    GGGGACAAAG AGCAGGATTC TAGAAATGTC CCAAGGAGAA ACGGTTTTGG GCAGAACAAC
    AAAGATCAAA TGCGAACGAA TGTTATTAAT GAGATCTTAA ATACAGAAAA ACATTATATT
    AAGCACCTGA AGGATATTTG TGAGGGCTAC ATCAAGCAGT GCCGTAAAAG GGACGACATG
    TTTACAGAAG ACCAACTATG GACAATCTTT GGAAACATTG AAGAGATCTA CAAATTCCAA
    AAGAAATTCT TGAAAACACT TGAAAAAAGA ATTAACAAAG ATTCCCCGCA TTTGAGTGAA
    ATCGGCTCTT GCTTTTTGGA ATATCAAACA GATTTCCAGA TCTACTCAGA ATATTGCAAC
    AATCATCCTA ATGCCTGCAC AGAACTTTCC AAACTCACCA AGGTCAAGAA GTATGTCCAT
    TTCTTTGAGA CTTGCCGTTT ATTACAGAAG ATGATTGATA TCTCTCTAGA TGGGTTTCTT
    CTAACCCCGG TACAGAAGAT CTGTAAATAT CCACTTCAGC TGGCGGAACT ACTAAAGTAC
    ACTAATTCCC AACACAGAGA CTACACAAAC GTTGAGGCAG CCTTAGATGC AATGAAAAAC
    GTTGCCAGAC TTATCAATGA AAGAAAGCGG AGACTTGAGA ACATTGATAA AATAGCCCAC
    TGGCAAAGTT CTATAGAGGA CTGGGAGGGA GAAGATATCC TTGCCAGAAG CTCGGAGCTC
    ATACATTCTG GGGAACTAAC CAAGATTTCT CACCTCCAGC CAAAGGGACA GCAGCGGATA
    TTCTTTCTGT TTGATCATCA AATTGTTTTC TGCAAGAAGG ATCTGTTGCG ACGTGACATT
    CTGTATTATA AAGGGAAGAT CGACATGGAC GATATGGAGG TTCTGAATGT AGAGGATGGG
    AAAGACAAAG ACTTTAACAT TACTGTAAAA AATGCATTCA AGCTGCAAGG CAAAGTCTCG
    GAAGAGATTC ACCTTTTCCT TGCAAAGAAA CCAGAGCACA AACAGCGATG GCTACAGGCC
    TTTGAGGAAG AAAGGAAACA GGTGCTATTG GATAAAGAGA CAGGTTTCAG CATCAGTGAA
    ATACAGAAGA AGCAGGCTAT GATTAATGCC AGTAAGCCTC ACCCGGCAGG AAAGCCTAAA
    GGTGTCAATA GGACATACTA TGATTTCTGG ATGAAACAAA AGCACCCCAC TCTGCCTGCC
    AATCTCCCAC AGCAACAAGT CTTCATGCTT GCCGAACCCA AGCGCAAACC CTCTAATTTC
    TGGCAGAATA TCAGCCGACT GACCCCGTTC AGAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002933398.2
    CDS1075..5433
    Translation

    Target ORF information:

    RefSeq Version XM_002933352.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002933352.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCAGAAA TGGACATGAA GAATTCAAAA GAAAAGGTGG GTGGTATTTT ATCTGATTCT 
    TTTCAAGACA TATTTAAAGG TTATTGGGAA GAGGACAGGC ACTCACGATC CAGTGAATCT
    GAAGAAAAAC AAAATATGAC ACTAGGCAGT ATTAAAAACA GCAGCCTGTT AAGGAAACAG
    GTTAGCAGAC AAGAGCCAAT GAGTACTGGC CATTTTGAAG ACTTTCAAGC TATTGGTGAC
    TCCTCAAGTA GCCTACAAGA CAGATCGCCT GTTATTGTAC TATCTGCCCA TCAGCCAAGA
    ACACAAACAG CATGGAAGGA AATTGAGCAG AATACATATA AACATGAAAA TGGCCATTCT
    TCACATTCAA ACAGCGATGA AAATTTAAGT GGTAGCAGAG AGTCTTTAAA CCAGGACCTA
    GTTTATAAAG CAAGATATTC AATGGGATGT GTGGAACAAG AAGAAGATGA GCAAAATGGC
    CCCACTTTCG TTGATATAAA CAGGGAAACT CCTCTCTTTC AAAAAGACTC AGAAACAATA
    TTTGTTCAAG AACATACAGA AGCCCATGGC AGGCACTTGT GGGACATATT GTTAGATAAA
    GAACAACACA ATCCCTCTCA AATAAGTATT CAAACAGCTA AAATAAAGTC CCTGAAGCAA
    AAAAGTTATA AGGATCCAAA CTACCATAGT AATGGTAATA TGGAGGATTC TAATCTTGAC
    CATGGTGACA GTGTCTTCTC TGCACAAAGA CGATATCTCA CAGCCCCACA AGATTCTGAT
    GGAGATGAAA GTGACTTTGA AGTTTCATCC TCGGAAGAAA ATAGAAATCA TTGTCTGGCA
    ACAGTTGAGG AGAGCAGAGA CGGCATGTGT TATTACAGTA CTATAAAGAA AATAGACACT
    ATAGACACAG TGGATGATAA TGAAAGCAAC TGCAGCAGCT CTAACAGTGA AGGCACTGAA
    GGCAACATGG GATACAACTG GAAAAGTGAG ATCAACAAAA TAGAAGACAT TAAAAGTGAC
    GTAAAGGCAT CAGAAAGCCA AAAAGACTCC AAAATAGCAG AATTAAAAGA TTCCAGAACA
    GAAATAAAAG AGGAGCCTAA CAATAAAATA AAACGGATGA ATATAAAAAA TAGCGACATT
    TTTAAAAAAT ATCTGTCGGT TAAAACACCT GTGCCAAATG GCTCACAACT CGCTGCTAAA
    CCTACAGGGG AAACGTCGGA CTTCTCTGAT GAGGATTCAT ATGAAAGTAG CTTTCTATAC
    AACAGGAGTA TGAGGAAGGC ATCAGGGTCC GGGAGAATTG ATCTAGATAA TTTTACCAGA
    TTAAGATGTG GTGTTGCGCC ATCAAATACC AATGATAAGT CAGACAATGG GCAAAGTCAG
    GGGTTCTCCA AACCCCCAAC AGAAAACTGT GAAACGAAGA GTGAAAGAAA CACCGAGAGT
    AAACGGTTAA AGGAAAGGTT GATGTGGGCG CATAAGTCTT TCTCTAGTAT GTTTGATTTT
    AAGAATAATG ATAAAGATAG TGCCGCCCAG AGTTCAGAGG GCACCCCTAG AGAGGAGAAG
    AAAAAGTCTA GGCCACTTCA GTCATCCTGG AAAACACTCC GAAATTCCAA AAACAAAGAT
    AAAGACTCAT TTAAGAGGCT CAGCGTGTTG AATTTATCAG CACATATTAT AAATACAAAC
    ATGCCAAGGG GGGCTTCAAA GGAAAATGGC AATGAACATC TACCCCCGAT TCTTGATCCA
    ATAAAACAAC CCTCCTCTAT GGAAATAAAG CCTTGTATGA CTACTGATAC AAACACAGAG
    TCAGATTTGG AACCTCTGGA AGCCAAGGAA AACCATAAAA AATGGAGCCT TGAAAATGAT
    GATTCTTTAC AAACTACTTG TGATGACTCA GCTTTTTTTT CACAATTTGA CTCAAACGAA
    TCACATTCGT TAAACAAACA GCCCTCTATT ACAACTTTAT TTAACCCATT CTCAAATTCT
    GAAATAAATC CCATGCCATG TCGACCAATG AGTCCAAAGC CTTTTAGCCA ATGGCCCAGC
    TTTCAGCGTA GGGGCTTGCA CAATTCCAGG ATAAGTGCTA CTTCGATGAC CTCTCTAGCA
    AACAGTTCTA CAGTGGATGG GTGCCCAGAT TCCCCAGAGA CAGCAGTAAA AACATGGGGA
    GGGTATTTAA CAGACAGAAG TAGTCAGAAG GATGACAGCA GTGCCAGCAG CCAATCAAAG
    CCCAGCCTTA ACACTGCTTC TTCTGTAATG GACATACTAA GAGATGAGGA TAGTAGACAA
    CTTGTCCCAT CATCGATACT AAAGATGCAA AGGGAAAGGA ATGCTTTGCT TTACAAGAAG
    AGACCTACAG ACCTGACGGC TTCTCAGTAC CTGCCTATCA ACACTGAAAG TAGAACACTG
    ACACTGCCCC AGATGGCTTC CATGGGACAC AAAAGGGATT CAGAACCAAC AAGGCCTAAA
    GCTTTATTCA AGAGCCTGAG TTGTGATAAC TTGTGGGTAT ATGAGAGGAA CCAAGAGAGG
    AAACTAGACA AGTCTATAGA TGCAGGAGAT CTCATGAGGA ACCAGTCAAA AGCTTCTATG
    AGTGTCTCTA TTGGGCCTCC AGAAATATTC CGAAGTTTCC CAATGAAGGT GCACTCGTTT
    TCGCGGAGCA CACCGAGCAG GCTTGATCTT GTGGGCTGTG TGAGGCGCAC CTCTCTCTCA
    GTATTCAATG ATGGGTCTAC GAACAGACCT TCGCTGTTTG ATGACTTGGG GAGTGATGAG
    GATTTTTATG AAGAACTACA CGTTTCTGGT CACCGCTTTG TTGGAGGGGG CGAGCAGCTG
    GCAATAAATG AGTTGATAAG TGATGGCAGT GTGGTATACG CCGAGGCTCT CTGGGACCAT
    GTCACTATGG ATGATGAAGA ACTGGGTTTT AAGGCCGGAA GTGTCATCGA AGTAATGGAT
    GCTACCAATA AAGAGTGGTG GTGGGGGAGG ATACTTGATA GTGAAGGATG GTTTCCAGCC
    AGCTTTGTTA GGCTCCGTGT CAACCAGGAT GAACCAATGG AAGACTGTGC TACTAAAGCA
    GGGGACAAAG AGCAGGATTC TAGAAATGTC CCAAGGAGAA ACGGTTTTGG GCAGAACAAC
    AAAGATCAAA TGCGAACGAA TGTTATTAAT GAGATCTTAA ATACAGAAAA ACATTATATT
    AAGCACCTGA AGGATATTTG TGAGGGCTAC ATCAAGCAGT GCCGTAAAAG GGACGACATG
    TTTACAGAAG ACCAACTATG GACAATCTTT GGAAACATTG AAGAGATCTA CAAATTCCAA
    AAGAAATTCT TGAAAACACT TGAAAAAAGA ATTAACAAAG ATTCCCCGCA TTTGAGTGAA
    ATCGGCTCTT GCTTTTTGGA ATATCAAACA GATTTCCAGA TCTACTCAGA ATATTGCAAC
    AATCATCCTA ATGCCTGCAC AGAACTTTCC AAACTCACCA AGGTCAAGAA GTATGTCCAT
    TTCTTTGAGA CTTGCCGTTT ATTACAGAAG ATGATTGATA TCTCTCTAGA TGGGTTTCTT
    CTAACCCCGG TACAGAAGAT CTGTAAATAT CCACTTCAGC TGGCGGAACT ACTAAAGTAC
    ACTAATTCCC AACACAGAGA CTACACAAAC GTTGAGGCAG CCTTAGATGC AATGAAAAAC
    GTTGCCAGAC TTATCAATGA AAGAAAGCGG AGACTTGAGA ACATTGATAA AATAGCCCAC
    TGGCAAAGTT CTATAGAGGA CTGGGAGGGA GAAGATATCC TTGCCAGAAG CTCGGAGCTC
    ATACATTCTG GGGAACTAAC CAAGATTTCT CACCTCCAGC CAAAGGGACA GCAGCGGATA
    TTCTTTCTGT TTGATCATCA AATTGTTTTC TGCAAGAAGG ATCTGTTGCG ACGTGACATT
    CTGTATTATA AAGGGAAGAT CGACATGGAC GATATGGAGG TTCTGAATGT AGAGGATGGG
    AAAGACAAAG ACTTTAACAT TACTGTAAAA AATGCATTCA AGCTGCAAGG CAAAGTCTCG
    GAAGAGATTC ACCTTTTCCT TGCAAAGAAA CCAGAGCACA AACAGCGATG GCTACAGGCC
    TTTGAGGAAG AAAGGAAACA GGTGCTATTG GATAAAGAGA CAGGTTTCAG CATCAGTGAA
    ATACAGAAGA AGCAGGCTAT GATTAATGCC AGTAAGCCTC ACCCGGCAGG AAAGCCTAAA
    GGTGTCAATA GGACATACTA TGATTTCTGG ATGAAACAAA AGCACCCCAC TCTGCCTGCC
    AATCTCCCAC AGCAACAAGT CTTCATGCTT GCCGAACCCA AGCGCAAACC CTCTAATTTC
    TGGCAGAATA TCAGCCGACT GACCCCGTTC AGAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.