arhgef4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgef4
Entrez Gene ID	100487089
Full Name	Rho guanine nucleotide exchange factor 4
General protein information	Preferred Names Rho guanine nucleotide exchange factor 4 Names rho guanine nucleotide exchange factor 4 Rho guanine nucleotide exchange factor (GEF) 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012970967.2	XP_012826421.1	rho guanine nucleotide exchange factor 4 isoform X3
XM_002933352.4	XP_002933398.2	rho guanine nucleotide exchange factor 4 isoform X2
XM_004917799.4	XP_004917856.1	rho guanine nucleotide exchange factor 4 isoform X1
XM_031902569.1	XP_031758429.1	rho guanine nucleotide exchange factor 4 isoform X2
XM_012970967.3	XP_012826421.1	rho guanine nucleotide exchange factor 4 isoform X4
XM_004917799.3	XP_004917856.1	rho guanine nucleotide exchange factor 4 isoform X1
XM_031902570.1	XP_031758430.1	rho guanine nucleotide exchange factor 4 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARHGEF4	NP_056135.2
Xenopus tropicalis (tropical clawed frog)	LOC100487089	XP_004917856.1
Bos taurus (cattle)	ARHGEF4	NP_001179002.1
Rattus norvegicus (Norway rat)	Arhgef4	XP_006244788.1
Danio rerio (zebrafish)	arhgef4	XP_687404.3
Pan troglodytes (chimpanzee)	ARHGEF4	XP_525919.2
Mus musculus (house mouse)	Arhgef4	NP_898840.2
	LOC703647	XP_002808100.1
Canis lupus familiaris (dog)	LOC483856	XP_540976.4
Gallus gallus (chicken)	ARHGEF4	XP_004937023.1

Gene Ontology

The following arhgef4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa33789	XM_012970967.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa58893	XM_031902570.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa58891	XM_004917799.4 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58892	XM_031902569.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa16058	XM_004917799.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa16059	XM_002933352.4 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OXa33789	XM_012970967.2	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgef4 ( XM_012970967.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826421.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa33789
Clone ID Related Accession (Same CDS sequence)	XM_012970967.2 , XM_012970967.3
Accession Version	XM_012970967.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCAG GTTACTTATC ACTGGGAAGG ACAGGCAGCT CAGCACTGAG CCAGCACTGC 
CAGGTACTTG TGCCTGCAAC AAGCAACAAG AGGCTTCTCT CAGCTGAGCT GTGCACCCAG 
TCTGCCCGGA TGAGGACTCG CCAATGCCCC AGTGATGCCA TGAACCCACA CAGCAGCAGT 
GGGAGTTCAG GGCTTTCCTC TGGCTCAGAT TCTGACAGCA GTAGCTGCCT TGGCTGGGGT 
GGTGCTGCTG CTGCTCCTGC TGCTGCTTCT GGGAGAGGAT TGCTGTCCAG ATACTGGAGC 
TTGGAAAGCC TTCACTCTGC TACCGTATTC AATGATGGGT CTACGAACAG ACCTTCGCTG 
TTTGATGACT TGGGGAGTGA TGAGGATTTT TATGAAGAAC TACACGTTTC TGGTCACCGC 
TTTGTTGGAG GGGGCGAGCA GCTGGCAATA AATGAGTTGA TAAGTGATGG CAGTGTGGTA 
TACGCCGAGG CTCTCTGGGA CCATGTCACT ATGGATGATG AAGAACTGGG TTTTAAGGCC 
GGAAGTGTCA TCGAAGTAAT GGATGCTACC AATAAAGAGT GGTGGTGGGG GAGGATACTT 
GATAGTGAAG GATGGTTTCC AGCCAGCTTT GTTAGGCTCC GTGTCAACCA GGATGAACCA 
ATGGAAGACT GTGCTACTAA AGCAGGGGAC AAAGAGCAGG ATTCTAGAAA TGTCCCAAGG 
AGAAACGGTT TTGGGCAGAA CAACAAAGAT CAAATGCGAA CGAATGTTAT TAATGAGATC 
TTAAATACAG AAAAACATTA TATTAAGCAC CTGAAGGATA TTTGTGAGGG CTACATCAAG 
CAGTGCCGTA AAAGGGACGA CATGTTTACA GAAGACCAAC TATGGACAAT CTTTGGAAAC 
ATTGAAGAGA TCTACAAATT CCAAAAGAAA TTCTTGAAAA CACTTGAAAA AAGAATTAAC 
AAAGATTCCC CGCATTTGAG TGAAATCGGC TCTTGCTTTT TGGAATATCA AACAGATTTC 
CAGATCTACT CAGAATATTG CAACAATCAT CCTAATGCCT GCACAGAACT TTCCAAACTC 
ACCAAGGTCA AGAAGTATGT CCATTTCTTT GAGACTTGCC GTTTATTACA GAAGATGATT 
GATATCTCTC TAGATGGGTT TCTTCTAACC CCGGTACAGA AGATCTGTAA ATATCCACTT 
CAGCTGGCGG AACTACTAAA GTACACTAAT TCCCAACACA GAGACTACAC AAACGTTGAG 
GCAGCCTTAG ATGCAATGAA AAACGTTGCC AGACTTATCA ATGAAAGAAA GCGGAGACTT 
GAGAACATTG ATAAAATAGC CCACTGGCAA AGTTCTATAG AGGACTGGGA GGGAGAAGAT 
ATCCTTGCCA GAAGCTCGGA GCTCATACAT TCTGGGGAAC TAACCAAGAT TTCTCACCTC 
CAGCCAAAGG GACAGCAGCG GATATTCTTT CTGTTTGATC ATCAAATTGT TTTCTGCAAG 
AAGGATCTGT TGCGACGTGA CATTCTGTAT TATAAAGGGA AGATCGACAT GGACGATATG 
GAGGTTCTGA ATGTAGAGGA TGGGAAAGAC AAAGACTTTA ACATTACTGT AAAAAATGCA 
TTCAAGCTGC AAGGCAAAGT CTCGGAAGAG ATTCACCTTT TCCTTGCAAA GAAACCAGAG 
CACAAACAGC GATGGCTACA GGCCTTTGAG GAAGAAAGGA AACAGGTGCT ATTGGATAAA 
GAGACAGGTT TCAGCATCAG TGAAATACAG AAGAAGCAGG CTATGATTAA TGCCAGTAAG 
CCTCACCCGG CAGGAAAGCC TAAAGGTGTC AATAGGACAT ACTATGATTT CTGGATGAAA 
CAAAAGCACC CCACTCTGCC TGCCAATCTC CCACAGCAAC AAGTCTTCAT GCTTGCCGAA 
CCCAAGCGCA AACCCTCTAA TTTCTGGCAG AATATCAGCC GACTGACCCC GTTCAGAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826421.1
CDS	42..2024
Translation	MMSGYLSLGRTGSSALSQHCQVLVPATSNKRLLSAELCTQSARMRTRQCPSDAMNPHSSSGSSGLSSGSDSDSSSCLGWGGAAAAPAAASGRGLLSRYWSLESLHSATVFNDGSTNRPSLFDDLGSDEDFYEELHVSGHRFVGGGEQLAINELISDGSVVYAEALWDHVTMDDEELGFKAGSVIEVMDATNKEWWWGRILDSEGWFPASFVRLRVNQDEPMEDCATKAGDKEQDSRNVPRRNGFGQNNKDQMRTNVINEILNTEKHYIKHLKDICEGYIKQCRKRDDMFTEDQLWTIFGNIEEIYKFQKKFLKTLEKRINKDSPHLSEIGSCFLEYQTDFQIYSEYCNNHPNACTELSKLTKVKKYVHFFETCRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNSQHRDYTNVEAALDAMKNVARLINERKRRLENIDKIAHWQSSIEDWEGEDILARSSELIHSGELTKISHLQPKGQQRIFFLFDHQIVFCKKDLLRRDILYYKGKIDMDDMEVLNVEDGKDKDFNITVKNAFKLQGKVSEEIHLFLAKKPEHKQRWLQAFEEERKQVLLDKETGFSISEIQKKQAMINASKPHPAGKPKGVNRTYYDFWMKQKHPTLPANLPQQQVFMLAEPKRKPSNFWQNISRLTPFRK

Target ORF information:

RefSeq Version	XM_012970967.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970967.2

ORF Insert Sequence:

ATGATGTCAG GTTACTTATC ACTGGGAAGG ACAGGCAGCT CAGCACTGAG CCAGCACTGC 
CAGGTACTTG TGCCTGCAAC AAGCAACAAG AGGCTTCTCT CAGCTGAGCT GTGCACCCAG 
TCTGCCCGGA TGAGGACTCG CCAATGCCCC AGTGATGCCA TGAACCCACA CAGCAGCAGT 
GGGAGTTCAG GGCTTTCCTC TGGCTCAGAT TCTGACAGCA GTAGCTGCCT TGGCTGGGGT 
GGTGCTGCTG CTGCTCCTGC TGCTGCTTCT GGGAGAGGAT TGCTGTCCAG ATACTGGAGC 
TTGGAAAGCC TTCACTCTGC TACCGTATTC AATGATGGGT CTACGAACAG ACCTTCGCTG 
TTTGATGACT TGGGGAGTGA TGAGGATTTT TATGAAGAAC TACACGTTTC TGGTCACCGC 
TTTGTTGGAG GGGGCGAGCA GCTGGCAATA AATGAGTTGA TAAGTGATGG CAGTGTGGTA 
TACGCCGAGG CTCTCTGGGA CCATGTCACT ATGGATGATG AAGAACTGGG TTTTAAGGCC 
GGAAGTGTCA TCGAAGTAAT GGATGCTACC AATAAAGAGT GGTGGTGGGG GAGGATACTT 
GATAGTGAAG GATGGTTTCC AGCCAGCTTT GTTAGGCTCC GTGTCAACCA GGATGAACCA 
ATGGAAGACT GTGCTACTAA AGCAGGGGAC AAAGAGCAGG ATTCTAGAAA TGTCCCAAGG 
AGAAACGGTT TTGGGCAGAA CAACAAAGAT CAAATGCGAA CGAATGTTAT TAATGAGATC 
TTAAATACAG AAAAACATTA TATTAAGCAC CTGAAGGATA TTTGTGAGGG CTACATCAAG 
CAGTGCCGTA AAAGGGACGA CATGTTTACA GAAGACCAAC TATGGACAAT CTTTGGAAAC 
ATTGAAGAGA TCTACAAATT CCAAAAGAAA TTCTTGAAAA CACTTGAAAA AAGAATTAAC 
AAAGATTCCC CGCATTTGAG TGAAATCGGC TCTTGCTTTT TGGAATATCA AACAGATTTC 
CAGATCTACT CAGAATATTG CAACAATCAT CCTAATGCCT GCACAGAACT TTCCAAACTC 
ACCAAGGTCA AGAAGTATGT CCATTTCTTT GAGACTTGCC GTTTATTACA GAAGATGATT 
GATATCTCTC TAGATGGGTT TCTTCTAACC CCGGTACAGA AGATCTGTAA ATATCCACTT 
CAGCTGGCGG AACTACTAAA GTACACTAAT TCCCAACACA GAGACTACAC AAACGTTGAG 
GCAGCCTTAG ATGCAATGAA AAACGTTGCC AGACTTATCA ATGAAAGAAA GCGGAGACTT 
GAGAACATTG ATAAAATAGC CCACTGGCAA AGTTCTATAG AGGACTGGGA GGGAGAAGAT 
ATCCTTGCCA GAAGCTCGGA GCTCATACAT TCTGGGGAAC TAACCAAGAT TTCTCACCTC 
CAGCCAAAGG GACAGCAGCG GATATTCTTT CTGTTTGATC ATCAAATTGT TTTCTGCAAG 
AAGGATCTGT TGCGACGTGA CATTCTGTAT TATAAAGGGA AGATCGACAT GGACGATATG 
GAGGTTCTGA ATGTAGAGGA TGGGAAAGAC AAAGACTTTA ACATTACTGT AAAAAATGCA 
TTCAAGCTGC AAGGCAAAGT CTCGGAAGAG ATTCACCTTT TCCTTGCAAA GAAACCAGAG 
CACAAACAGC GATGGCTACA GGCCTTTGAG GAAGAAAGGA AACAGGTGCT ATTGGATAAA 
GAGACAGGTT TCAGCATCAG TGAAATACAG AAGAAGCAGG CTATGATTAA TGCCAGTAAG 
CCTCACCCGG CAGGAAAGCC TAAAGGTGTC AATAGGACAT ACTATGATTT CTGGATGAAA 
CAAAAGCACC CCACTCTGCC TGCCAATCTC CCACAGCAAC AAGTCTTCAT GCTTGCCGAA 
CCCAAGCGCA AACCCTCTAA TTTCTGGCAG AATATCAGCC GACTGACCCC GTTCAGAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef4 ( XM_012970967.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826421.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa33789
Clone ID Related Accession (Same CDS sequence)	XM_012970967.2 , XM_012970967.3
Accession Version	XM_012970967.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970967.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCAG GTTACTTATC ACTGGGAAGG ACAGGCAGCT CAGCACTGAG CCAGCACTGC 
CAGGTACTTG TGCCTGCAAC AAGCAACAAG AGGCTTCTCT CAGCTGAGCT GTGCACCCAG 
TCTGCCCGGA TGAGGACTCG CCAATGCCCC AGTGATGCCA TGAACCCACA CAGCAGCAGT 
GGGAGTTCAG GGCTTTCCTC TGGCTCAGAT TCTGACAGCA GTAGCTGCCT TGGCTGGGGT 
GGTGCTGCTG CTGCTCCTGC TGCTGCTTCT GGGAGAGGAT TGCTGTCCAG ATACTGGAGC 
TTGGAAAGCC TTCACTCTGC TACCGTATTC AATGATGGGT CTACGAACAG ACCTTCGCTG 
TTTGATGACT TGGGGAGTGA TGAGGATTTT TATGAAGAAC TACACGTTTC TGGTCACCGC 
TTTGTTGGAG GGGGCGAGCA GCTGGCAATA AATGAGTTGA TAAGTGATGG CAGTGTGGTA 
TACGCCGAGG CTCTCTGGGA CCATGTCACT ATGGATGATG AAGAACTGGG TTTTAAGGCC 
GGAAGTGTCA TCGAAGTAAT GGATGCTACC AATAAAGAGT GGTGGTGGGG GAGGATACTT 
GATAGTGAAG GATGGTTTCC AGCCAGCTTT GTTAGGCTCC GTGTCAACCA GGATGAACCA 
ATGGAAGACT GTGCTACTAA AGCAGGGGAC AAAGAGCAGG ATTCTAGAAA TGTCCCAAGG 
AGAAACGGTT TTGGGCAGAA CAACAAAGAT CAAATGCGAA CGAATGTTAT TAATGAGATC 
TTAAATACAG AAAAACATTA TATTAAGCAC CTGAAGGATA TTTGTGAGGG CTACATCAAG 
CAGTGCCGTA AAAGGGACGA CATGTTTACA GAAGACCAAC TATGGACAAT CTTTGGAAAC 
ATTGAAGAGA TCTACAAATT CCAAAAGAAA TTCTTGAAAA CACTTGAAAA AAGAATTAAC 
AAAGATTCCC CGCATTTGAG TGAAATCGGC TCTTGCTTTT TGGAATATCA AACAGATTTC 
CAGATCTACT CAGAATATTG CAACAATCAT CCTAATGCCT GCACAGAACT TTCCAAACTC 
ACCAAGGTCA AGAAGTATGT CCATTTCTTT GAGACTTGCC GTTTATTACA GAAGATGATT 
GATATCTCTC TAGATGGGTT TCTTCTAACC CCGGTACAGA AGATCTGTAA ATATCCACTT 
CAGCTGGCGG AACTACTAAA GTACACTAAT TCCCAACACA GAGACTACAC AAACGTTGAG 
GCAGCCTTAG ATGCAATGAA AAACGTTGCC AGACTTATCA ATGAAAGAAA GCGGAGACTT 
GAGAACATTG ATAAAATAGC CCACTGGCAA AGTTCTATAG AGGACTGGGA GGGAGAAGAT 
ATCCTTGCCA GAAGCTCGGA GCTCATACAT TCTGGGGAAC TAACCAAGAT TTCTCACCTC 
CAGCCAAAGG GACAGCAGCG GATATTCTTT CTGTTTGATC ATCAAATTGT TTTCTGCAAG 
AAGGATCTGT TGCGACGTGA CATTCTGTAT TATAAAGGGA AGATCGACAT GGACGATATG 
GAGGTTCTGA ATGTAGAGGA TGGGAAAGAC AAAGACTTTA ACATTACTGT AAAAAATGCA 
TTCAAGCTGC AAGGCAAAGT CTCGGAAGAG ATTCACCTTT TCCTTGCAAA GAAACCAGAG 
CACAAACAGC GATGGCTACA GGCCTTTGAG GAAGAAAGGA AACAGGTGCT ATTGGATAAA 
GAGACAGGTT TCAGCATCAG TGAAATACAG AAGAAGCAGG CTATGATTAA TGCCAGTAAG 
CCTCACCCGG CAGGAAAGCC TAAAGGTGTC AATAGGACAT ACTATGATTT CTGGATGAAA 
CAAAAGCACC CCACTCTGCC TGCCAATCTC CCACAGCAAC AAGTCTTCAT GCTTGCCGAA 
CCCAAGCGCA AACCCTCTAA TTTCTGGCAG AATATCAGCC GACTGACCCC GTTCAGAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826421.1
CDS	174..2156
Translation	MMSGYLSLGRTGSSALSQHCQVLVPATSNKRLLSAELCTQSARMRTRQCPSDAMNPHSSSGSSGLSSGSDSDSSSCLGWGGAAAAPAAASGRGLLSRYWSLESLHSATVFNDGSTNRPSLFDDLGSDEDFYEELHVSGHRFVGGGEQLAINELISDGSVVYAEALWDHVTMDDEELGFKAGSVIEVMDATNKEWWWGRILDSEGWFPASFVRLRVNQDEPMEDCATKAGDKEQDSRNVPRRNGFGQNNKDQMRTNVINEILNTEKHYIKHLKDICEGYIKQCRKRDDMFTEDQLWTIFGNIEEIYKFQKKFLKTLEKRINKDSPHLSEIGSCFLEYQTDFQIYSEYCNNHPNACTELSKLTKVKKYVHFFETCRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNSQHRDYTNVEAALDAMKNVARLINERKRRLENIDKIAHWQSSIEDWEGEDILARSSELIHSGELTKISHLQPKGQQRIFFLFDHQIVFCKKDLLRRDILYYKGKIDMDDMEVLNVEDGKDKDFNITVKNAFKLQGKVSEEIHLFLAKKPEHKQRWLQAFEEERKQVLLDKETGFSISEIQKKQAMINASKPHPAGKPKGVNRTYYDFWMKQKHPTLPANLPQQQVFMLAEPKRKPSNFWQNISRLTPFRK

Target ORF information:

RefSeq Version	XM_012970967.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970967.3

ORF Insert Sequence:

ATGATGTCAG GTTACTTATC ACTGGGAAGG ACAGGCAGCT CAGCACTGAG CCAGCACTGC 
CAGGTACTTG TGCCTGCAAC AAGCAACAAG AGGCTTCTCT CAGCTGAGCT GTGCACCCAG 
TCTGCCCGGA TGAGGACTCG CCAATGCCCC AGTGATGCCA TGAACCCACA CAGCAGCAGT 
GGGAGTTCAG GGCTTTCCTC TGGCTCAGAT TCTGACAGCA GTAGCTGCCT TGGCTGGGGT 
GGTGCTGCTG CTGCTCCTGC TGCTGCTTCT GGGAGAGGAT TGCTGTCCAG ATACTGGAGC 
TTGGAAAGCC TTCACTCTGC TACCGTATTC AATGATGGGT CTACGAACAG ACCTTCGCTG 
TTTGATGACT TGGGGAGTGA TGAGGATTTT TATGAAGAAC TACACGTTTC TGGTCACCGC 
TTTGTTGGAG GGGGCGAGCA GCTGGCAATA AATGAGTTGA TAAGTGATGG CAGTGTGGTA 
TACGCCGAGG CTCTCTGGGA CCATGTCACT ATGGATGATG AAGAACTGGG TTTTAAGGCC 
GGAAGTGTCA TCGAAGTAAT GGATGCTACC AATAAAGAGT GGTGGTGGGG GAGGATACTT 
GATAGTGAAG GATGGTTTCC AGCCAGCTTT GTTAGGCTCC GTGTCAACCA GGATGAACCA 
ATGGAAGACT GTGCTACTAA AGCAGGGGAC AAAGAGCAGG ATTCTAGAAA TGTCCCAAGG 
AGAAACGGTT TTGGGCAGAA CAACAAAGAT CAAATGCGAA CGAATGTTAT TAATGAGATC 
TTAAATACAG AAAAACATTA TATTAAGCAC CTGAAGGATA TTTGTGAGGG CTACATCAAG 
CAGTGCCGTA AAAGGGACGA CATGTTTACA GAAGACCAAC TATGGACAAT CTTTGGAAAC 
ATTGAAGAGA TCTACAAATT CCAAAAGAAA TTCTTGAAAA CACTTGAAAA AAGAATTAAC 
AAAGATTCCC CGCATTTGAG TGAAATCGGC TCTTGCTTTT TGGAATATCA AACAGATTTC 
CAGATCTACT CAGAATATTG CAACAATCAT CCTAATGCCT GCACAGAACT TTCCAAACTC 
ACCAAGGTCA AGAAGTATGT CCATTTCTTT GAGACTTGCC GTTTATTACA GAAGATGATT 
GATATCTCTC TAGATGGGTT TCTTCTAACC CCGGTACAGA AGATCTGTAA ATATCCACTT 
CAGCTGGCGG AACTACTAAA GTACACTAAT TCCCAACACA GAGACTACAC AAACGTTGAG 
GCAGCCTTAG ATGCAATGAA AAACGTTGCC AGACTTATCA ATGAAAGAAA GCGGAGACTT 
GAGAACATTG ATAAAATAGC CCACTGGCAA AGTTCTATAG AGGACTGGGA GGGAGAAGAT 
ATCCTTGCCA GAAGCTCGGA GCTCATACAT TCTGGGGAAC TAACCAAGAT TTCTCACCTC 
CAGCCAAAGG GACAGCAGCG GATATTCTTT CTGTTTGATC ATCAAATTGT TTTCTGCAAG 
AAGGATCTGT TGCGACGTGA CATTCTGTAT TATAAAGGGA AGATCGACAT GGACGATATG 
GAGGTTCTGA ATGTAGAGGA TGGGAAAGAC AAAGACTTTA ACATTACTGT AAAAAATGCA 
TTCAAGCTGC AAGGCAAAGT CTCGGAAGAG ATTCACCTTT TCCTTGCAAA GAAACCAGAG 
CACAAACAGC GATGGCTACA GGCCTTTGAG GAAGAAAGGA AACAGGTGCT ATTGGATAAA 
GAGACAGGTT TCAGCATCAG TGAAATACAG AAGAAGCAGG CTATGATTAA TGCCAGTAAG 
CCTCACCCGG CAGGAAAGCC TAAAGGTGTC AATAGGACAT ACTATGATTT CTGGATGAAA 
CAAAAGCACC CCACTCTGCC TGCCAATCTC CCACAGCAAC AAGTCTTCAT GCTTGCCGAA 
CCCAAGCGCA AACCCTCTAA TTTCTGGCAG AATATCAGCC GACTGACCCC GTTCAGAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef4 ( XM_031902570.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758430.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa58893
Clone ID Related Accession (Same CDS sequence)	XM_031902570.1
Accession Version	XM_031902570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATGC AGTGGAAAGG AATGGCTACT TTAGAAGGAA ACAATCATTT CTCCAAGCAC 
CAGGATAGTA GACAACTTGT CCCATCATCG ATACTAAAGA TGCAAAGGGA AAGGAATGCT 
TTGCTTTACA AGAAGAGACC TACAGACCTG ACGGCTTCTC AGTACCTGCC TATCAACACT 
GAAAGTAGAA CACTGACACT GCCCCAGATG GCTTCCATGG GACACAAAAG GGATTCAGAA 
CCAACAAGGC CTAAAGCTTT ATTCAAGAGC CTGAGTTGTG ATAACTTGTG GGTATATGAG 
AGGAACCAAG AGAGGAAACT AGACAAGTCT ATAGATGCAG GAGATCTCAT GAGGAACCAG 
TCAAAAGCTT CTATGAGTGT CTCTATTGGG CCTCCAGAAA TATTCCGAAG TTTCCCAATG 
AAGGTGCACT CGTTTTCGCG GAGCACACCG AGCAGGCTTG ATCTTGTGGG CTGTGTGAGG 
CGCACCTCTC TCTCAGTATT CAATGATGGG TCTACGAACA GACCTTCGCT GTTTGATGAC 
TTGGGGAGTG ATGAGGATTT TTATGAAGAA CTACACGTTT CTGGTCACCG CTTTGTTGGA 
GGGGGCGAGC AGCTGGCAAT AAATGAGTTG ATAAGTGATG GCAGTGTGGT ATACGCCGAG 
GCTCTCTGGG ACCATGTCAC TATGGATGAT GAAGAACTGG GTTTTAAGGC CGGAAGTGTC 
ATCGAAGTAA TGGATGCTAC CAATAAAGAG TGGTGGTGGG GGAGGATACT TGATAGTGAA 
GGATGGTTTC CAGCCAGCTT TGTTAGGCTC CGTGTCAACC AGGATGAACC AATGGAAGAC 
TGTGCTACTA AAGCAGGGGA CAAAGAGCAG GATTCTAGAA ATGTCCCAAG GAGAAACGGT 
TTTGGGCAGA ACAACAAAGA TCAAATGCGA ACGAATGTTA TTAATGAGAT CTTAAATACA 
GAAAAACATT ATATTAAGCA CCTGAAGGAT ATTTGTGAGG GCTACATCAA GCAGTGCCGT 
AAAAGGGACG ACATGTTTAC AGAAGACCAA CTATGGACAA TCTTTGGAAA CATTGAAGAG 
ATCTACAAAT TCCAAAAGAA ATTCTTGAAA ACACTTGAAA AAAGAATTAA CAAAGATTCC 
CCGCATTTGA GTGAAATCGG CTCTTGCTTT TTGGAATATC AAACAGATTT CCAGATCTAC 
TCAGAATATT GCAACAATCA TCCTAATGCC TGCACAGAAC TTTCCAAACT CACCAAGGTC 
AAGAAGTATG TCCATTTCTT TGAGACTTGC CGTTTATTAC AGAAGATGAT TGATATCTCT 
CTAGATGGGT TTCTTCTAAC CCCGGTACAG AAGATCTGTA AATATCCACT TCAGCTGGCG 
GAACTACTAA AGTACACTAA TTCCCAACAC AGAGACTACA CAAACGTTGA GGCAGCCTTA 
GATGCAATGA AAAACGTTGC CAGACTTATC AATGAAAGAA AGCGGAGACT TGAGAACATT 
GATAAAATAG CCCACTGGCA AAGTTCTATA GAGGACTGGG AGGGAGAAGA TATCCTTGCC 
AGAAGCTCGG AGCTCATACA TTCTGGGGAA CTAACCAAGA TTTCTCACCT CCAGCCAAAG 
GGACAGCAGC GGATATTCTT TCTGTTTGAT CATCAAATTG TTTTCTGCAA GAAGGATCTG 
TTGCGACGTG ACATTCTGTA TTATAAAGGG AAGATCGACA TGGACGATAT GGAGGTTCTG 
AATGTAGAGG ATGGGAAAGA CAAAGACTTT AACATTACTG TAAAAAATGC ATTCAAGCTG 
CAAGGCAAAG TCTCGGAAGA GATTCACCTT TTCCTTGCAA AGAAACCAGA GCACAAACAG 
CGATGGCTAC AGGCCTTTGA GGAAGAAAGG AAACAGGTGC TATTGGATAA AGAGACAGGT 
TTCAGCATCA GTGAAATACA GAAGAAGCAG GCTATGATTA ATGCCAGTAA GCCTCACCCG 
GCAGGAAAGC CTAAAGGTGT CAATAGGACA TACTATGATT TCTGGATGAA ACAAAAGCAC 
CCCACTCTGC CTGCCAATCT CCCACAGCAA CAAGTCTTCA TGCTTGCCGA ACCCAAGCGC 
AAACCCTCTA ATTTCTGGCA GAATATCAGC CGACTGACCC CGTTCAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758430.1
CDS	16..2169
Translation	MRMQWKGMATLEGNNHFSKHQDSRQLVPSSILKMQRERNALLYKKRPTDLTASQYLPINTESRTLTLPQMASMGHKRDSEPTRPKALFKSLSCDNLWVYERNQERKLDKSIDAGDLMRNQSKASMSVSIGPPEIFRSFPMKVHSFSRSTPSRLDLVGCVRRTSLSVFNDGSTNRPSLFDDLGSDEDFYEELHVSGHRFVGGGEQLAINELISDGSVVYAEALWDHVTMDDEELGFKAGSVIEVMDATNKEWWWGRILDSEGWFPASFVRLRVNQDEPMEDCATKAGDKEQDSRNVPRRNGFGQNNKDQMRTNVINEILNTEKHYIKHLKDICEGYIKQCRKRDDMFTEDQLWTIFGNIEEIYKFQKKFLKTLEKRINKDSPHLSEIGSCFLEYQTDFQIYSEYCNNHPNACTELSKLTKVKKYVHFFETCRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNSQHRDYTNVEAALDAMKNVARLINERKRRLENIDKIAHWQSSIEDWEGEDILARSSELIHSGELTKISHLQPKGQQRIFFLFDHQIVFCKKDLLRRDILYYKGKIDMDDMEVLNVEDGKDKDFNITVKNAFKLQGKVSEEIHLFLAKKPEHKQRWLQAFEEERKQVLLDKETGFSISEIQKKQAMINASKPHPAGKPKGVNRTYYDFWMKQKHPTLPANLPQQQVFMLAEPKRKPSNFWQNISRLTPFRK

Target ORF information:

RefSeq Version	XM_031902570.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902570.1

ORF Insert Sequence:

ATGAGAATGC AGTGGAAAGG AATGGCTACT TTAGAAGGAA ACAATCATTT CTCCAAGCAC 
CAGGATAGTA GACAACTTGT CCCATCATCG ATACTAAAGA TGCAAAGGGA AAGGAATGCT 
TTGCTTTACA AGAAGAGACC TACAGACCTG ACGGCTTCTC AGTACCTGCC TATCAACACT 
GAAAGTAGAA CACTGACACT GCCCCAGATG GCTTCCATGG GACACAAAAG GGATTCAGAA 
CCAACAAGGC CTAAAGCTTT ATTCAAGAGC CTGAGTTGTG ATAACTTGTG GGTATATGAG 
AGGAACCAAG AGAGGAAACT AGACAAGTCT ATAGATGCAG GAGATCTCAT GAGGAACCAG 
TCAAAAGCTT CTATGAGTGT CTCTATTGGG CCTCCAGAAA TATTCCGAAG TTTCCCAATG 
AAGGTGCACT CGTTTTCGCG GAGCACACCG AGCAGGCTTG ATCTTGTGGG CTGTGTGAGG 
CGCACCTCTC TCTCAGTATT CAATGATGGG TCTACGAACA GACCTTCGCT GTTTGATGAC 
TTGGGGAGTG ATGAGGATTT TTATGAAGAA CTACACGTTT CTGGTCACCG CTTTGTTGGA 
GGGGGCGAGC AGCTGGCAAT AAATGAGTTG ATAAGTGATG GCAGTGTGGT ATACGCCGAG 
GCTCTCTGGG ACCATGTCAC TATGGATGAT GAAGAACTGG GTTTTAAGGC CGGAAGTGTC 
ATCGAAGTAA TGGATGCTAC CAATAAAGAG TGGTGGTGGG GGAGGATACT TGATAGTGAA 
GGATGGTTTC CAGCCAGCTT TGTTAGGCTC CGTGTCAACC AGGATGAACC AATGGAAGAC 
TGTGCTACTA AAGCAGGGGA CAAAGAGCAG GATTCTAGAA ATGTCCCAAG GAGAAACGGT 
TTTGGGCAGA ACAACAAAGA TCAAATGCGA ACGAATGTTA TTAATGAGAT CTTAAATACA 
GAAAAACATT ATATTAAGCA CCTGAAGGAT ATTTGTGAGG GCTACATCAA GCAGTGCCGT 
AAAAGGGACG ACATGTTTAC AGAAGACCAA CTATGGACAA TCTTTGGAAA CATTGAAGAG 
ATCTACAAAT TCCAAAAGAA ATTCTTGAAA ACACTTGAAA AAAGAATTAA CAAAGATTCC 
CCGCATTTGA GTGAAATCGG CTCTTGCTTT TTGGAATATC AAACAGATTT CCAGATCTAC 
TCAGAATATT GCAACAATCA TCCTAATGCC TGCACAGAAC TTTCCAAACT CACCAAGGTC 
AAGAAGTATG TCCATTTCTT TGAGACTTGC CGTTTATTAC AGAAGATGAT TGATATCTCT 
CTAGATGGGT TTCTTCTAAC CCCGGTACAG AAGATCTGTA AATATCCACT TCAGCTGGCG 
GAACTACTAA AGTACACTAA TTCCCAACAC AGAGACTACA CAAACGTTGA GGCAGCCTTA 
GATGCAATGA AAAACGTTGC CAGACTTATC AATGAAAGAA AGCGGAGACT TGAGAACATT 
GATAAAATAG CCCACTGGCA AAGTTCTATA GAGGACTGGG AGGGAGAAGA TATCCTTGCC 
AGAAGCTCGG AGCTCATACA TTCTGGGGAA CTAACCAAGA TTTCTCACCT CCAGCCAAAG 
GGACAGCAGC GGATATTCTT TCTGTTTGAT CATCAAATTG TTTTCTGCAA GAAGGATCTG 
TTGCGACGTG ACATTCTGTA TTATAAAGGG AAGATCGACA TGGACGATAT GGAGGTTCTG 
AATGTAGAGG ATGGGAAAGA CAAAGACTTT AACATTACTG TAAAAAATGC ATTCAAGCTG 
CAAGGCAAAG TCTCGGAAGA GATTCACCTT TTCCTTGCAA AGAAACCAGA GCACAAACAG 
CGATGGCTAC AGGCCTTTGA GGAAGAAAGG AAACAGGTGC TATTGGATAA AGAGACAGGT 
TTCAGCATCA GTGAAATACA GAAGAAGCAG GCTATGATTA ATGCCAGTAA GCCTCACCCG 
GCAGGAAAGC CTAAAGGTGT CAATAGGACA TACTATGATT TCTGGATGAA ACAAAAGCAC 
CCCACTCTGC CTGCCAATCT CCCACAGCAA CAAGTCTTCA TGCTTGCCGA ACCCAAGCGC 
AAACCCTCTA ATTTCTGGCA GAATATCAGC CGACTGACCC CGTTCAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef4 ( XM_004917799.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917856.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X1, mRNA.

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CloneID	OXa58891
Clone ID Related Accession (Same CDS sequence)	XM_004917799.4
Accession Version	XM_004917799.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917799.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG 
GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC 
TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT 
AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA 
CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT 
CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC 
CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA 
CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT 
ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA 
TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA 
TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC 
CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT 
GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA 
GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA 
CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT 
TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA 
AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG 
GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA 
TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA 
AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA 
GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC 
CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA 
TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC 
AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA 
AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT 
GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA 
CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT 
CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG 
GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT 
GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT 
GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG 
AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG 
GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA 
TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA 
GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG 
GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT 
CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA 
ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG 
AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT 
GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA 
CCCCCCACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG 
GAAAGGTTGA TGTGGGCGCA TAAATCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT 
AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG 
CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT 
AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG 
GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC 
TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA 
CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA 
ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA 
AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC 
ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG 
GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA 
GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA 
GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC 
ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGATA GTAGACAACT TGTCCCATCA 
TCGATACTAA AGATGCAAAG GGAAAGGAAT GCTTTGCTTT ACAAGAAGAG ACCTACAGAC 
CTGACGGCTT CTCAGTACCT GCCTATCAAC ACTGAAAGTA GAACACTGAC ACTGCCCCAG 
ATGGCTTCCA TGGGACACAA AAGGGATTCA GAACCAACAA GGCCTAAAGC TTTATTCAAG 
AGCCTGAGTT GTGATAACTT GTGGGTATAT GAGAGGAACC AAGAGAGGAA ACTAGACAAG 
TCTATAGATG CAGGAGATCT CATGAGGAAC CAGTCAAAAG CTTCTATGAG TGTCTCTATT 
GGGCCTCCAG AAATATTCCG AAGTTTCCCA ATGAAGGTGC ACTCGTTTTC GCGGAGCACA 
CCGAGCAGGC TTGATCTTGT GGGCTGTGTG AGGCGCACCT CTCTCTCAGT ATTCAATGAT 
GGGTCTACGA ACAGACCTTC GCTGTTTGAT GACTTGGGGA GTGATGAGGA TTTTTATGAA 
GAACTACACG TTTCTGGTCA CCGCTTTGTT GGAGGGGGCG AGCAGCTGGC AATAAATGAG 
TTGATAAGTG ATGGCAGTGT GGTATACGCC GAGGCTCTCT GGGACCATGT CACTATGGAT 
GATGAAGAAC TGGGTTTTAA GGCCGGAAGT GTCATCGAAG TAATGGATGC TACCAATAAA 
GAGTGGTGGT GGGGGAGGAT ACTTGATAGT GAAGGATGGT TTCCAGCCAG CTTTGTTAGG 
CTCCGTGTCA ACCAGGATGA ACCAATGGAA GACTGTGCTA CTAAAGCAGG GGACAAAGAG 
CAGGATTCTA GAAATGTCCC AAGGAGAAAC GGTTTTGGGC AGAACAACAA AGATCAAATG 
CGAACGAATG TTATTAATGA GATCTTAAAT ACAGAAAAAC ATTATATTAA GCACCTGAAG 
GATATTTGTG AGGGCTACAT CAAGCAGTGC CGTAAAAGGG ACGACATGTT TACAGAAGAC 
CAACTATGGA CAATCTTTGG AAACATTGAA GAGATCTACA AATTCCAAAA GAAATTCTTG 
AAAACACTTG AAAAAAGAAT TAACAAAGAT TCCCCGCATT TGAGTGAAAT CGGCTCTTGC 
TTTTTGGAAT ATCAAACAGA TTTCCAGATC TACTCAGAAT ATTGCAACAA TCATCCTAAT 
GCCTGCACAG AACTTTCCAA ACTCACCAAG GTCAAGAAGT ATGTCCATTT CTTTGAGACT 
TGCCGTTTAT TACAGAAGAT GATTGATATC TCTCTAGATG GGTTTCTTCT AACCCCGGTA 
CAGAAGATCT GTAAATATCC ACTTCAGCTG GCGGAACTAC TAAAGTACAC TAATTCCCAA 
CACAGAGACT ACACAAACGT TGAGGCAGCC TTAGATGCAA TGAAAAACGT TGCCAGACTT 
ATCAATGAAA GAAAGCGGAG ACTTGAGAAC ATTGATAAAA TAGCCCACTG GCAAAGTTCT 
ATAGAGGACT GGGAGGGAGA AGATATCCTT GCCAGAAGCT CGGAGCTCAT ACATTCTGGG 
GAACTAACCA AGATTTCTCA CCTCCAGCCA AAGGGACAGC AGCGGATATT CTTTCTGTTT 
GATCATCAAA TTGTTTTCTG CAAGAAGGAT CTGTTGCGAC GTGACATTCT GTATTATAAA 
GGGAAGATCG ACATGGACGA TATGGAGGTT CTGAATGTAG AGGATGGGAA AGACAAAGAC 
TTTAACATTA CTGTAAAAAA TGCATTCAAG CTGCAAGGCA AAGTCTCGGA AGAGATTCAC 
CTTTTCCTTG CAAAGAAACC AGAGCACAAA CAGCGATGGC TACAGGCCTT TGAGGAAGAA 
AGGAAACAGG TGCTATTGGA TAAAGAGACA GGTTTCAGCA TCAGTGAAAT ACAGAAGAAG 
CAGGCTATGA TTAATGCCAG TAAGCCTCAC CCGGCAGGAA AGCCTAAAGG TGTCAATAGG 
ACATACTATG ATTTCTGGAT GAAACAAAAG CACCCCACTC TGCCTGCCAA TCTCCCACAG 
CAACAAGTCT TCATGCTTGC CGAACCCAAG CGCAAACCCT CTAATTTCTG GCAGAATATC 
AGCCGACTGA CCCCGTTCAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917856.1
CDS	178..5604
Translation	MLSVMYFLRSFFKTPEPNEDSNTKLGFNEDHWEEPCLQEVGTFGQEQDEDYFETRSNFSDCPSELKSEVFESISDAESVASEANGELIRRSDCLFLEKEEPLYSLNNDDNCLSSNIVESSHEECSKNAPAETASESNVLHHALTYDQEHLDIINDNEVTQLTDLFSPDSKQIGVSSNIIDISGSESNRSSHKDVFQCQAGLQLTGTLISAKLHGEIANSKSENCLNCTESERSIGTGVSSQSLPNISFDISHYSDVSSKHGIASALTQSSQQTCLKTSSLEEINLDCYLNSVSQDVGLLIHTETEKHISKDIVHLQDSYLSSQLCEEEHLLSPAGHNEFQSTLCVLADDRKETKGCMPEMDMKNSKEKVGGILSDSFQDIFKGYWEEDRHSRSSESEEKQNMTLGSIKNSSLLRKQVSRQEPMSTGHFEDFQAIGDSSSSLQDRSPVIVLSAHQPRTQTAWKEIEQNTYKHENGHSSHSNSDENLSGSRESLNQDLVYKARYSMGCVEQEEDEQNGPTFVDINRETPLFQKDSETIFVQEHTEAHGRHLWDILLDKEQHNPSQISIQTAKIKSLKQKSYKDPNYHSNGNMEDSNLDHGDSVFSAQRRYLTAPQDSDGDESDFEVSSSEENRNHCLATVEESRDGMCYYSTIKKIDTIDTVDDNESNCSSSNSEGTEGNMGYNWKSEINKIEDIKSDVKASESQKDSKIAELKDSRTEIKEEPNNKIKRMNIKNSDIFKKYLSVKTPVPNGSQLAAKPTGETSDFSDEDSYESSFLYNRSMRKASGSGRIDLDNFTRLRCGVAPSNTNDKSDNGQSQGFSKPPTENCETKSERNTESKRLKERLMWAHKSFSSMFDFKNNDKDSAAQSSEGTPREEKKKSRPLQSSWKTLRNSKNKDKDSFKRLSVLNLSAHIINTNMPRGASKENGNEHLPPILDPIKQPSSMEIKPCMTTDTNTESDLEPLEAKENHKKWSLENDDSLQTTCDDSAFFSQFDSNESHSLNKQPSITTLFNPFSNSEINPMPCRPMSPKPFSQWPSFQRRGLHNSRISATSMTSLANSSTVDGCPDSPETAVKTWGGYLTDRSSQKDDSSASSQSKPSLNTASSVMDILRDEDSRQLVPSSILKMQRERNALLYKKRPTDLTASQYLPINTESRTLTLPQMASMGHKRDSEPTRPKALFKSLSCDNLWVYERNQERKLDKSIDAGDLMRNQSKASMSVSIGPPEIFRSFPMKVHSFSRSTPSRLDLVGCVRRTSLSVFNDGSTNRPSLFDDLGSDEDFYEELHVSGHRFVGGGEQLAINELISDGSVVYAEALWDHVTMDDEELGFKAGSVIEVMDATNKEWWWGRILDSEGWFPASFVRLRVNQDEPMEDCATKAGDKEQDSRNVPRRNGFGQNNKDQMRTNVINEILNTEKHYIKHLKDICEGYIKQCRKRDDMFTEDQLWTIFGNIEEIYKFQKKFLKTLEKRINKDSPHLSEIGSCFLEYQTDFQIYSEYCNNHPNACTELSKLTKVKKYVHFFETCRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNSQHRDYTNVEAALDAMKNVARLINERKRRLENIDKIAHWQSSIEDWEGEDILARSSELIHSGELTKISHLQPKGQQRIFFLFDHQIVFCKKDLLRRDILYYKGKIDMDDMEVLNVEDGKDKDFNITVKNAFKLQGKVSEEIHLFLAKKPEHKQRWLQAFEEERKQVLLDKETGFSISEIQKKQAMINASKPHPAGKPKGVNRTYYDFWMKQKHPTLPANLPQQQVFMLAEPKRKPSNFWQNISRLTPFRK

Target ORF information:

RefSeq Version	XM_004917799.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917799.4

ORF Insert Sequence:

ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG 
GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC 
TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT 
AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA 
CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT 
CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC 
CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA 
CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT 
ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA 
TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA 
TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC 
CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT 
GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA 
GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA 
CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT 
TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA 
AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG 
GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA 
TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA 
AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA 
GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC 
CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA 
TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC 
AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA 
AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT 
GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA 
CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT 
CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG 
GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT 
GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT 
GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG 
AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG 
GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA 
TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA 
GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG 
GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT 
CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA 
ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG 
AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT 
GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA 
CCCCCCACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG 
GAAAGGTTGA TGTGGGCGCA TAAATCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT 
AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG 
CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT 
AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG 
GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC 
TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA 
CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA 
ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA 
AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC 
ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG 
GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA 
GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA 
GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC 
ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGATA GTAGACAACT TGTCCCATCA 
TCGATACTAA AGATGCAAAG GGAAAGGAAT GCTTTGCTTT ACAAGAAGAG ACCTACAGAC 
CTGACGGCTT CTCAGTACCT GCCTATCAAC ACTGAAAGTA GAACACTGAC ACTGCCCCAG 
ATGGCTTCCA TGGGACACAA AAGGGATTCA GAACCAACAA GGCCTAAAGC TTTATTCAAG 
AGCCTGAGTT GTGATAACTT GTGGGTATAT GAGAGGAACC AAGAGAGGAA ACTAGACAAG 
TCTATAGATG CAGGAGATCT CATGAGGAAC CAGTCAAAAG CTTCTATGAG TGTCTCTATT 
GGGCCTCCAG AAATATTCCG AAGTTTCCCA ATGAAGGTGC ACTCGTTTTC GCGGAGCACA 
CCGAGCAGGC TTGATCTTGT GGGCTGTGTG AGGCGCACCT CTCTCTCAGT ATTCAATGAT 
GGGTCTACGA ACAGACCTTC GCTGTTTGAT GACTTGGGGA GTGATGAGGA TTTTTATGAA 
GAACTACACG TTTCTGGTCA CCGCTTTGTT GGAGGGGGCG AGCAGCTGGC AATAAATGAG 
TTGATAAGTG ATGGCAGTGT GGTATACGCC GAGGCTCTCT GGGACCATGT CACTATGGAT 
GATGAAGAAC TGGGTTTTAA GGCCGGAAGT GTCATCGAAG TAATGGATGC TACCAATAAA 
GAGTGGTGGT GGGGGAGGAT ACTTGATAGT GAAGGATGGT TTCCAGCCAG CTTTGTTAGG 
CTCCGTGTCA ACCAGGATGA ACCAATGGAA GACTGTGCTA CTAAAGCAGG GGACAAAGAG 
CAGGATTCTA GAAATGTCCC AAGGAGAAAC GGTTTTGGGC AGAACAACAA AGATCAAATG 
CGAACGAATG TTATTAATGA GATCTTAAAT ACAGAAAAAC ATTATATTAA GCACCTGAAG 
GATATTTGTG AGGGCTACAT CAAGCAGTGC CGTAAAAGGG ACGACATGTT TACAGAAGAC 
CAACTATGGA CAATCTTTGG AAACATTGAA GAGATCTACA AATTCCAAAA GAAATTCTTG 
AAAACACTTG AAAAAAGAAT TAACAAAGAT TCCCCGCATT TGAGTGAAAT CGGCTCTTGC 
TTTTTGGAAT ATCAAACAGA TTTCCAGATC TACTCAGAAT ATTGCAACAA TCATCCTAAT 
GCCTGCACAG AACTTTCCAA ACTCACCAAG GTCAAGAAGT ATGTCCATTT CTTTGAGACT 
TGCCGTTTAT TACAGAAGAT GATTGATATC TCTCTAGATG GGTTTCTTCT AACCCCGGTA 
CAGAAGATCT GTAAATATCC ACTTCAGCTG GCGGAACTAC TAAAGTACAC TAATTCCCAA 
CACAGAGACT ACACAAACGT TGAGGCAGCC TTAGATGCAA TGAAAAACGT TGCCAGACTT 
ATCAATGAAA GAAAGCGGAG ACTTGAGAAC ATTGATAAAA TAGCCCACTG GCAAAGTTCT 
ATAGAGGACT GGGAGGGAGA AGATATCCTT GCCAGAAGCT CGGAGCTCAT ACATTCTGGG 
GAACTAACCA AGATTTCTCA CCTCCAGCCA AAGGGACAGC AGCGGATATT CTTTCTGTTT 
GATCATCAAA TTGTTTTCTG CAAGAAGGAT CTGTTGCGAC GTGACATTCT GTATTATAAA 
GGGAAGATCG ACATGGACGA TATGGAGGTT CTGAATGTAG AGGATGGGAA AGACAAAGAC 
TTTAACATTA CTGTAAAAAA TGCATTCAAG CTGCAAGGCA AAGTCTCGGA AGAGATTCAC 
CTTTTCCTTG CAAAGAAACC AGAGCACAAA CAGCGATGGC TACAGGCCTT TGAGGAAGAA 
AGGAAACAGG TGCTATTGGA TAAAGAGACA GGTTTCAGCA TCAGTGAAAT ACAGAAGAAG 
CAGGCTATGA TTAATGCCAG TAAGCCTCAC CCGGCAGGAA AGCCTAAAGG TGTCAATAGG 
ACATACTATG ATTTCTGGAT GAAACAAAAG CACCCCACTC TGCCTGCCAA TCTCCCACAG 
CAACAAGTCT TCATGCTTGC CGAACCCAAG CGCAAACCCT CTAATTTCTG GCAGAATATC 
AGCCGACTGA CCCCGTTCAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef4 ( XM_031902569.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758429.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X2, mRNA.

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CloneID	OXa58892
Clone ID Related Accession (Same CDS sequence)	XM_031902569.1
Accession Version	XM_031902569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG 
GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC 
TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT 
AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA 
CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT 
CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC 
CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA 
CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT 
ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA 
TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA 
TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC 
CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT 
GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA 
GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA 
CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT 
TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA 
AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG 
GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA 
TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA 
AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA 
GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC 
CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA 
TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC 
AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA 
AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT 
GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA 
CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT 
CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG 
GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT 
GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT 
GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG 
AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG 
GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA 
TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA 
GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG 
GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT 
CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA 
ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG 
AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT 
GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA 
CCCCCCACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG 
GAAAGGTTGA TGTGGGCGCA TAAATCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT 
AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG 
CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT 
AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG 
GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC 
TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA 
CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA 
ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA 
AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC 
ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG 
GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA 
GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA 
GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC 
ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGCTT CTATGAGTGT CTCTATTGGG 
CCTCCAGAAA TATTCCGAAG TTTCCCAATG AAGGTGCACT CGTTTTCGCG GAGCACACCG 
AGCAGGCTTG ATCTTGTGGG CTGTGTGAGG CGCACCTCTC TCTCAGTATT CAATGATGGG 
TCTACGAACA GACCTTCGCT GTTTGATGAC TTGGGGAGTG ATGAGGATTT TTATGAAGAA 
CTACACGTTT CTGGTCACCG CTTTGTTGGA GGGGGCGAGC AGCTGGCAAT AAATGAGTTG 
ATAAGTGATG GCAGTGTGGT ATACGCCGAG GCTCTCTGGG ACCATGTCAC TATGGATGAT 
GAAGAACTGG GTTTTAAGGC CGGAAGTGTC ATCGAAGTAA TGGATGCTAC CAATAAAGAG 
TGGTGGTGGG GGAGGATACT TGATAGTGAA GGATGGTTTC CAGCCAGCTT TGTTAGGCTC 
CGTGTCAACC AGGATGAACC AATGGAAGAC TGTGCTACTA AAGCAGGGGA CAAAGAGCAG 
GATTCTAGAA ATGTCCCAAG GAGAAACGGT TTTGGGCAGA ACAACAAAGA TCAAATGCGA 
ACGAATGTTA TTAATGAGAT CTTAAATACA GAAAAACATT ATATTAAGCA CCTGAAGGAT 
ATTTGTGAGG GCTACATCAA GCAGTGCCGT AAAAGGGACG ACATGTTTAC AGAAGACCAA 
CTATGGACAA TCTTTGGAAA CATTGAAGAG ATCTACAAAT TCCAAAAGAA ATTCTTGAAA 
ACACTTGAAA AAAGAATTAA CAAAGATTCC CCGCATTTGA GTGAAATCGG CTCTTGCTTT 
TTGGAATATC AAACAGATTT CCAGATCTAC TCAGAATATT GCAACAATCA TCCTAATGCC 
TGCACAGAAC TTTCCAAACT CACCAAGGTC AAGAAGTATG TCCATTTCTT TGAGACTTGC 
CGTTTATTAC AGAAGATGAT TGATATCTCT CTAGATGGGT TTCTTCTAAC CCCGGTACAG 
AAGATCTGTA AATATCCACT TCAGCTGGCG GAACTACTAA AGTACACTAA TTCCCAACAC 
AGAGACTACA CAAACGTTGA GGCAGCCTTA GATGCAATGA AAAACGTTGC CAGACTTATC 
AATGAAAGAA AGCGGAGACT TGAGAACATT GATAAAATAG CCCACTGGCA AAGTTCTATA 
GAGGACTGGG AGGGAGAAGA TATCCTTGCC AGAAGCTCGG AGCTCATACA TTCTGGGGAA 
CTAACCAAGA TTTCTCACCT CCAGCCAAAG GGACAGCAGC GGATATTCTT TCTGTTTGAT 
CATCAAATTG TTTTCTGCAA GAAGGATCTG TTGCGACGTG ACATTCTGTA TTATAAAGGG 
AAGATCGACA TGGACGATAT GGAGGTTCTG AATGTAGAGG ATGGGAAAGA CAAAGACTTT 
AACATTACTG TAAAAAATGC ATTCAAGCTG CAAGGCAAAG TCTCGGAAGA GATTCACCTT 
TTCCTTGCAA AGAAACCAGA GCACAAACAG CGATGGCTAC AGGCCTTTGA GGAAGAAAGG 
AAACAGGTGC TATTGGATAA AGAGACAGGT TTCAGCATCA GTGAAATACA GAAGAAGCAG 
GCTATGATTA ATGCCAGTAA GCCTCACCCG GCAGGAAAGC CTAAAGGTGT CAATAGGACA 
TACTATGATT TCTGGATGAA ACAAAAGCAC CCCACTCTGC CTGCCAATCT CCCACAGCAA 
CAAGTCTTCA TGCTTGCCGA ACCCAAGCGC AAACCCTCTA ATTTCTGGCA GAATATCAGC 
CGACTGACCC CGTTCAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758429.1
CDS	178..5301
Translation	MLSVMYFLRSFFKTPEPNEDSNTKLGFNEDHWEEPCLQEVGTFGQEQDEDYFETRSNFSDCPSELKSEVFESISDAESVASEANGELIRRSDCLFLEKEEPLYSLNNDDNCLSSNIVESSHEECSKNAPAETASESNVLHHALTYDQEHLDIINDNEVTQLTDLFSPDSKQIGVSSNIIDISGSESNRSSHKDVFQCQAGLQLTGTLISAKLHGEIANSKSENCLNCTESERSIGTGVSSQSLPNISFDISHYSDVSSKHGIASALTQSSQQTCLKTSSLEEINLDCYLNSVSQDVGLLIHTETEKHISKDIVHLQDSYLSSQLCEEEHLLSPAGHNEFQSTLCVLADDRKETKGCMPEMDMKNSKEKVGGILSDSFQDIFKGYWEEDRHSRSSESEEKQNMTLGSIKNSSLLRKQVSRQEPMSTGHFEDFQAIGDSSSSLQDRSPVIVLSAHQPRTQTAWKEIEQNTYKHENGHSSHSNSDENLSGSRESLNQDLVYKARYSMGCVEQEEDEQNGPTFVDINRETPLFQKDSETIFVQEHTEAHGRHLWDILLDKEQHNPSQISIQTAKIKSLKQKSYKDPNYHSNGNMEDSNLDHGDSVFSAQRRYLTAPQDSDGDESDFEVSSSEENRNHCLATVEESRDGMCYYSTIKKIDTIDTVDDNESNCSSSNSEGTEGNMGYNWKSEINKIEDIKSDVKASESQKDSKIAELKDSRTEIKEEPNNKIKRMNIKNSDIFKKYLSVKTPVPNGSQLAAKPTGETSDFSDEDSYESSFLYNRSMRKASGSGRIDLDNFTRLRCGVAPSNTNDKSDNGQSQGFSKPPTENCETKSERNTESKRLKERLMWAHKSFSSMFDFKNNDKDSAAQSSEGTPREEKKKSRPLQSSWKTLRNSKNKDKDSFKRLSVLNLSAHIINTNMPRGASKENGNEHLPPILDPIKQPSSMEIKPCMTTDTNTESDLEPLEAKENHKKWSLENDDSLQTTCDDSAFFSQFDSNESHSLNKQPSITTLFNPFSNSEINPMPCRPMSPKPFSQWPSFQRRGLHNSRISATSMTSLANSSTVDGCPDSPETAVKTWGGYLTDRSSQKDDSSASSQSKPSLNTASSVMDILRDEASMSVSIGPPEIFRSFPMKVHSFSRSTPSRLDLVGCVRRTSLSVFNDGSTNRPSLFDDLGSDEDFYEELHVSGHRFVGGGEQLAINELISDGSVVYAEALWDHVTMDDEELGFKAGSVIEVMDATNKEWWWGRILDSEGWFPASFVRLRVNQDEPMEDCATKAGDKEQDSRNVPRRNGFGQNNKDQMRTNVINEILNTEKHYIKHLKDICEGYIKQCRKRDDMFTEDQLWTIFGNIEEIYKFQKKFLKTLEKRINKDSPHLSEIGSCFLEYQTDFQIYSEYCNNHPNACTELSKLTKVKKYVHFFETCRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNSQHRDYTNVEAALDAMKNVARLINERKRRLENIDKIAHWQSSIEDWEGEDILARSSELIHSGELTKISHLQPKGQQRIFFLFDHQIVFCKKDLLRRDILYYKGKIDMDDMEVLNVEDGKDKDFNITVKNAFKLQGKVSEEIHLFLAKKPEHKQRWLQAFEEERKQVLLDKETGFSISEIQKKQAMINASKPHPAGKPKGVNRTYYDFWMKQKHPTLPANLPQQQVFMLAEPKRKPSNFWQNISRLTPFRK

Target ORF information:

RefSeq Version	XM_031902569.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902569.1

ORF Insert Sequence:

ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG 
GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC 
TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT 
AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA 
CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT 
CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC 
CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA 
CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT 
ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA 
TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA 
TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC 
CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT 
GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA 
GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA 
CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT 
TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA 
AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG 
GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA 
TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA 
AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA 
GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC 
CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA 
TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC 
AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA 
AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT 
GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA 
CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT 
CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG 
GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT 
GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT 
GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG 
AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG 
GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA 
TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA 
GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG 
GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT 
CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA 
ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG 
AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT 
GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA 
CCCCCCACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG 
GAAAGGTTGA TGTGGGCGCA TAAATCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT 
AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG 
CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT 
AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG 
GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC 
TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA 
CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA 
ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA 
AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC 
ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG 
GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA 
GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA 
GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC 
ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGCTT CTATGAGTGT CTCTATTGGG 
CCTCCAGAAA TATTCCGAAG TTTCCCAATG AAGGTGCACT CGTTTTCGCG GAGCACACCG 
AGCAGGCTTG ATCTTGTGGG CTGTGTGAGG CGCACCTCTC TCTCAGTATT CAATGATGGG 
TCTACGAACA GACCTTCGCT GTTTGATGAC TTGGGGAGTG ATGAGGATTT TTATGAAGAA 
CTACACGTTT CTGGTCACCG CTTTGTTGGA GGGGGCGAGC AGCTGGCAAT AAATGAGTTG 
ATAAGTGATG GCAGTGTGGT ATACGCCGAG GCTCTCTGGG ACCATGTCAC TATGGATGAT 
GAAGAACTGG GTTTTAAGGC CGGAAGTGTC ATCGAAGTAA TGGATGCTAC CAATAAAGAG 
TGGTGGTGGG GGAGGATACT TGATAGTGAA GGATGGTTTC CAGCCAGCTT TGTTAGGCTC 
CGTGTCAACC AGGATGAACC AATGGAAGAC TGTGCTACTA AAGCAGGGGA CAAAGAGCAG 
GATTCTAGAA ATGTCCCAAG GAGAAACGGT TTTGGGCAGA ACAACAAAGA TCAAATGCGA 
ACGAATGTTA TTAATGAGAT CTTAAATACA GAAAAACATT ATATTAAGCA CCTGAAGGAT 
ATTTGTGAGG GCTACATCAA GCAGTGCCGT AAAAGGGACG ACATGTTTAC AGAAGACCAA 
CTATGGACAA TCTTTGGAAA CATTGAAGAG ATCTACAAAT TCCAAAAGAA ATTCTTGAAA 
ACACTTGAAA AAAGAATTAA CAAAGATTCC CCGCATTTGA GTGAAATCGG CTCTTGCTTT 
TTGGAATATC AAACAGATTT CCAGATCTAC TCAGAATATT GCAACAATCA TCCTAATGCC 
TGCACAGAAC TTTCCAAACT CACCAAGGTC AAGAAGTATG TCCATTTCTT TGAGACTTGC 
CGTTTATTAC AGAAGATGAT TGATATCTCT CTAGATGGGT TTCTTCTAAC CCCGGTACAG 
AAGATCTGTA AATATCCACT TCAGCTGGCG GAACTACTAA AGTACACTAA TTCCCAACAC 
AGAGACTACA CAAACGTTGA GGCAGCCTTA GATGCAATGA AAAACGTTGC CAGACTTATC 
AATGAAAGAA AGCGGAGACT TGAGAACATT GATAAAATAG CCCACTGGCA AAGTTCTATA 
GAGGACTGGG AGGGAGAAGA TATCCTTGCC AGAAGCTCGG AGCTCATACA TTCTGGGGAA 
CTAACCAAGA TTTCTCACCT CCAGCCAAAG GGACAGCAGC GGATATTCTT TCTGTTTGAT 
CATCAAATTG TTTTCTGCAA GAAGGATCTG TTGCGACGTG ACATTCTGTA TTATAAAGGG 
AAGATCGACA TGGACGATAT GGAGGTTCTG AATGTAGAGG ATGGGAAAGA CAAAGACTTT 
AACATTACTG TAAAAAATGC ATTCAAGCTG CAAGGCAAAG TCTCGGAAGA GATTCACCTT 
TTCCTTGCAA AGAAACCAGA GCACAAACAG CGATGGCTAC AGGCCTTTGA GGAAGAAAGG 
AAACAGGTGC TATTGGATAA AGAGACAGGT TTCAGCATCA GTGAAATACA GAAGAAGCAG 
GCTATGATTA ATGCCAGTAA GCCTCACCCG GCAGGAAAGC CTAAAGGTGT CAATAGGACA 
TACTATGATT TCTGGATGAA ACAAAAGCAC CCCACTCTGC CTGCCAATCT CCCACAGCAA 
CAAGTCTTCA TGCTTGCCGA ACCCAAGCGC AAACCCTCTA ATTTCTGGCA GAATATCAGC 
CGACTGACCC CGTTCAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef4 ( XM_004917799.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917856.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X1, mRNA.

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CloneID	OXa16058
Clone ID Related Accession (Same CDS sequence)	XM_004917799.3
Accession Version	XM_004917799.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917799.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG 
GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC 
TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT 
AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA 
CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT 
CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC 
CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA 
CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT 
ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA 
TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA 
TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC 
CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT 
GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA 
GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA 
CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT 
TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA 
AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG 
GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA 
TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA 
AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA 
GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC 
CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA 
TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC 
AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA 
AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT 
GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA 
CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT 
CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG 
GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT 
GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT 
GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG 
AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG 
GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA 
TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA 
GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG 
GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT 
CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA 
ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG 
AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT 
GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA 
CCCCCAACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG 
GAAAGGTTGA TGTGGGCGCA TAAGTCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT 
AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG 
CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT 
AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG 
GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC 
TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA 
CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA 
ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA 
AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC 
ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG 
GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA 
GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA 
GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC 
ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGATA GTAGACAACT TGTCCCATCA 
TCGATACTAA AGATGCAAAG GGAAAGGAAT GCTTTGCTTT ACAAGAAGAG ACCTACAGAC 
CTGACGGCTT CTCAGTACCT GCCTATCAAC ACTGAAAGTA GAACACTGAC ACTGCCCCAG 
ATGGCTTCCA TGGGACACAA AAGGGATTCA GAACCAACAA GGCCTAAAGC TTTATTCAAG 
AGCCTGAGTT GTGATAACTT GTGGGTATAT GAGAGGAACC AAGAGAGGAA ACTAGACAAG 
TCTATAGATG CAGGAGATCT CATGAGGAAC CAGTCAAAAG CTTCTATGAG TGTCTCTATT 
GGGCCTCCAG AAATATTCCG AAGTTTCCCA ATGAAGGTGC ACTCGTTTTC GCGGAGCACA 
CCGAGCAGGC TTGATCTTGT GGGCTGTGTG AGGCGCACCT CTCTCTCAGT ATTCAATGAT 
GGGTCTACGA ACAGACCTTC GCTGTTTGAT GACTTGGGGA GTGATGAGGA TTTTTATGAA 
GAACTACACG TTTCTGGTCA CCGCTTTGTT GGAGGGGGCG AGCAGCTGGC AATAAATGAG 
TTGATAAGTG ATGGCAGTGT GGTATACGCC GAGGCTCTCT GGGACCATGT CACTATGGAT 
GATGAAGAAC TGGGTTTTAA GGCCGGAAGT GTCATCGAAG TAATGGATGC TACCAATAAA 
GAGTGGTGGT GGGGGAGGAT ACTTGATAGT GAAGGATGGT TTCCAGCCAG CTTTGTTAGG 
CTCCGTGTCA ACCAGGATGA ACCAATGGAA GACTGTGCTA CTAAAGCAGG GGACAAAGAG 
CAGGATTCTA GAAATGTCCC AAGGAGAAAC GGTTTTGGGC AGAACAACAA AGATCAAATG 
CGAACGAATG TTATTAATGA GATCTTAAAT ACAGAAAAAC ATTATATTAA GCACCTGAAG 
GATATTTGTG AGGGCTACAT CAAGCAGTGC CGTAAAAGGG ACGACATGTT TACAGAAGAC 
CAACTATGGA CAATCTTTGG AAACATTGAA GAGATCTACA AATTCCAAAA GAAATTCTTG 
AAAACACTTG AAAAAAGAAT TAACAAAGAT TCCCCGCATT TGAGTGAAAT CGGCTCTTGC 
TTTTTGGAAT ATCAAACAGA TTTCCAGATC TACTCAGAAT ATTGCAACAA TCATCCTAAT 
GCCTGCACAG AACTTTCCAA ACTCACCAAG GTCAAGAAGT ATGTCCATTT CTTTGAGACT 
TGCCGTTTAT TACAGAAGAT GATTGATATC TCTCTAGATG GGTTTCTTCT AACCCCGGTA 
CAGAAGATCT GTAAATATCC ACTTCAGCTG GCGGAACTAC TAAAGTACAC TAATTCCCAA 
CACAGAGACT ACACAAACGT TGAGGCAGCC TTAGATGCAA TGAAAAACGT TGCCAGACTT 
ATCAATGAAA GAAAGCGGAG ACTTGAGAAC ATTGATAAAA TAGCCCACTG GCAAAGTTCT 
ATAGAGGACT GGGAGGGAGA AGATATCCTT GCCAGAAGCT CGGAGCTCAT ACATTCTGGG 
GAACTAACCA AGATTTCTCA CCTCCAGCCA AAGGGACAGC AGCGGATATT CTTTCTGTTT 
GATCATCAAA TTGTTTTCTG CAAGAAGGAT CTGTTGCGAC GTGACATTCT GTATTATAAA 
GGGAAGATCG ACATGGACGA TATGGAGGTT CTGAATGTAG AGGATGGGAA AGACAAAGAC 
TTTAACATTA CTGTAAAAAA TGCATTCAAG CTGCAAGGCA AAGTCTCGGA AGAGATTCAC 
CTTTTCCTTG CAAAGAAACC AGAGCACAAA CAGCGATGGC TACAGGCCTT TGAGGAAGAA 
AGGAAACAGG TGCTATTGGA TAAAGAGACA GGTTTCAGCA TCAGTGAAAT ACAGAAGAAG 
CAGGCTATGA TTAATGCCAG TAAGCCTCAC CCGGCAGGAA AGCCTAAAGG TGTCAATAGG 
ACATACTATG ATTTCTGGAT GAAACAAAAG CACCCCACTC TGCCTGCCAA TCTCCCACAG 
CAACAAGTCT TCATGCTTGC CGAACCCAAG CGCAAACCCT CTAATTTCTG GCAGAATATC 
AGCCGACTGA CCCCGTTCAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917856.1
CDS	165..5591
Translation	MLSVMYFLRSFFKTPEPNEDSNTKLGFNEDHWEEPCLQEVGTFGQEQDEDYFETRSNFSDCPSELKSEVFESISDAESVASEANGELIRRSDCLFLEKEEPLYSLNNDDNCLSSNIVESSHEECSKNAPAETASESNVLHHALTYDQEHLDIINDNEVTQLTDLFSPDSKQIGVSSNIIDISGSESNRSSHKDVFQCQAGLQLTGTLISAKLHGEIANSKSENCLNCTESERSIGTGVSSQSLPNISFDISHYSDVSSKHGIASALTQSSQQTCLKTSSLEEINLDCYLNSVSQDVGLLIHTETEKHISKDIVHLQDSYLSSQLCEEEHLLSPAGHNEFQSTLCVLADDRKETKGCMPEMDMKNSKEKVGGILSDSFQDIFKGYWEEDRHSRSSESEEKQNMTLGSIKNSSLLRKQVSRQEPMSTGHFEDFQAIGDSSSSLQDRSPVIVLSAHQPRTQTAWKEIEQNTYKHENGHSSHSNSDENLSGSRESLNQDLVYKARYSMGCVEQEEDEQNGPTFVDINRETPLFQKDSETIFVQEHTEAHGRHLWDILLDKEQHNPSQISIQTAKIKSLKQKSYKDPNYHSNGNMEDSNLDHGDSVFSAQRRYLTAPQDSDGDESDFEVSSSEENRNHCLATVEESRDGMCYYSTIKKIDTIDTVDDNESNCSSSNSEGTEGNMGYNWKSEINKIEDIKSDVKASESQKDSKIAELKDSRTEIKEEPNNKIKRMNIKNSDIFKKYLSVKTPVPNGSQLAAKPTGETSDFSDEDSYESSFLYNRSMRKASGSGRIDLDNFTRLRCGVAPSNTNDKSDNGQSQGFSKPPTENCETKSERNTESKRLKERLMWAHKSFSSMFDFKNNDKDSAAQSSEGTPREEKKKSRPLQSSWKTLRNSKNKDKDSFKRLSVLNLSAHIINTNMPRGASKENGNEHLPPILDPIKQPSSMEIKPCMTTDTNTESDLEPLEAKENHKKWSLENDDSLQTTCDDSAFFSQFDSNESHSLNKQPSITTLFNPFSNSEINPMPCRPMSPKPFSQWPSFQRRGLHNSRISATSMTSLANSSTVDGCPDSPETAVKTWGGYLTDRSSQKDDSSASSQSKPSLNTASSVMDILRDEDSRQLVPSSILKMQRERNALLYKKRPTDLTASQYLPINTESRTLTLPQMASMGHKRDSEPTRPKALFKSLSCDNLWVYERNQERKLDKSIDAGDLMRNQSKASMSVSIGPPEIFRSFPMKVHSFSRSTPSRLDLVGCVRRTSLSVFNDGSTNRPSLFDDLGSDEDFYEELHVSGHRFVGGGEQLAINELISDGSVVYAEALWDHVTMDDEELGFKAGSVIEVMDATNKEWWWGRILDSEGWFPASFVRLRVNQDEPMEDCATKAGDKEQDSRNVPRRNGFGQNNKDQMRTNVINEILNTEKHYIKHLKDICEGYIKQCRKRDDMFTEDQLWTIFGNIEEIYKFQKKFLKTLEKRINKDSPHLSEIGSCFLEYQTDFQIYSEYCNNHPNACTELSKLTKVKKYVHFFETCRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNSQHRDYTNVEAALDAMKNVARLINERKRRLENIDKIAHWQSSIEDWEGEDILARSSELIHSGELTKISHLQPKGQQRIFFLFDHQIVFCKKDLLRRDILYYKGKIDMDDMEVLNVEDGKDKDFNITVKNAFKLQGKVSEEIHLFLAKKPEHKQRWLQAFEEERKQVLLDKETGFSISEIQKKQAMINASKPHPAGKPKGVNRTYYDFWMKQKHPTLPANLPQQQVFMLAEPKRKPSNFWQNISRLTPFRK

Target ORF information:

RefSeq Version	XM_004917799.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917799.3

ORF Insert Sequence:

ATGCTAAGTG TAATGTACTT TCTGCGGAGC TTCTTTAAGA CCCCTGAGCC CAACGAAGAC 
TCTAACACTA AATTAGGTTT CAACGAAGAC CATTGGGAAG AACCATGCTT GCAAGAGGTG 
GGAACTTTCG GTCAGGAACA AGATGAAGAT TATTTTGAAA CTAGGTCAAA TTTCAGTGAC 
TGTCCATCAG AATTAAAAAG TGAAGTATTT GAAAGTATTT CAGACGCTGA GTCAGTTGCT 
AGTGAAGCGA ATGGTGAACT AATCAGGAGA TCTGATTGCC TTTTCCTGGA AAAAGAAGAA 
CCTTTGTATT CATTGAATAA CGATGACAAC TGCCTTTCTT CCAACATCGT TGAGAGCAGT 
CATGAAGAAT GCAGTAAAAA TGCGCCGGCC GAGACTGCTT CTGAATCAAA TGTTTTACAC 
CACGCTTTAA CATATGACCA AGAACATTTG GATATAATTA ATGACAATGA GGTCACTCAA 
CTAACGGATT TATTTAGCCC AGATTCAAAA CAAATAGGTG TTAGCTCAAA TATTATAGAT 
ATCAGTGGAT CAGAAAGTAA CAGATCCAGT CATAAGGATG TATTTCAATG CCAGGCAGGA 
TTACAATTAA CTGGAACATT AATATCTGCC AAACTACATG GAGAAATTGC AAATTCAAAA 
TCAGAAAACT GCCTTAATTG TACAGAGTCA GAGAGATCCA TTGGTACAGG TGTGTCCAGC 
CAAAGCTTGC CTAATATCAG TTTTGACATT TCTCATTATT CTGACGTCTC TAGCAAGCAT 
GGAATTGCAA GTGCACTAAC ACAGAGTAGC CAGCAAACAT GTTTAAAGAC TTCATCTCTA 
GAGGAAATTA ATCTCGACTG TTATTTAAAT TCTGTATCTC AGGATGTGGG ACTCTTGATA 
CATACTGAGA CAGAAAAACA CATTTCCAAG GATATTGTTC ATTTGCAAGA TTCCTATCTT 
TCTTCTCAGC TATGTGAGGA GGAACATCTG TTATCACCTG CAGGACACAA TGAATTTCAA 
AGCACTCTGT GTGTTTTAGC TGATGATAGA AAAGAAACTA AAGGTTGCAT GCCAGAAATG 
GACATGAAGA ATTCAAAAGA AAAGGTGGGT GGTATTTTAT CTGATTCTTT TCAAGACATA 
TTTAAAGGTT ATTGGGAAGA GGACAGGCAC TCACGATCCA GTGAATCTGA AGAAAAACAA 
AATATGACAC TAGGCAGTAT TAAAAACAGC AGCCTGTTAA GGAAACAGGT TAGCAGACAA 
GAGCCAATGA GTACTGGCCA TTTTGAAGAC TTTCAAGCTA TTGGTGACTC CTCAAGTAGC 
CTACAAGACA GATCGCCTGT TATTGTACTA TCTGCCCATC AGCCAAGAAC ACAAACAGCA 
TGGAAGGAAA TTGAGCAGAA TACATATAAA CATGAAAATG GCCATTCTTC ACATTCAAAC 
AGCGATGAAA ATTTAAGTGG TAGCAGAGAG TCTTTAAACC AGGACCTAGT TTATAAAGCA 
AGATATTCAA TGGGATGTGT GGAACAAGAA GAAGATGAGC AAAATGGCCC CACTTTCGTT 
GATATAAACA GGGAAACTCC TCTCTTTCAA AAAGACTCAG AAACAATATT TGTTCAAGAA 
CATACAGAAG CCCATGGCAG GCACTTGTGG GACATATTGT TAGATAAAGA ACAACACAAT 
CCCTCTCAAA TAAGTATTCA AACAGCTAAA ATAAAGTCCC TGAAGCAAAA AAGTTATAAG 
GATCCAAACT ACCATAGTAA TGGTAATATG GAGGATTCTA ATCTTGACCA TGGTGACAGT 
GTCTTCTCTG CACAAAGACG ATATCTCACA GCCCCACAAG ATTCTGATGG AGATGAAAGT 
GACTTTGAAG TTTCATCCTC GGAAGAAAAT AGAAATCATT GTCTGGCAAC AGTTGAGGAG 
AGCAGAGACG GCATGTGTTA TTACAGTACT ATAAAGAAAA TAGACACTAT AGACACAGTG 
GATGATAATG AAAGCAACTG CAGCAGCTCT AACAGTGAAG GCACTGAAGG CAACATGGGA 
TACAACTGGA AAAGTGAGAT CAACAAAATA GAAGACATTA AAAGTGACGT AAAGGCATCA 
GAAAGCCAAA AAGACTCCAA AATAGCAGAA TTAAAAGATT CCAGAACAGA AATAAAAGAG 
GAGCCTAACA ATAAAATAAA ACGGATGAAT ATAAAAAATA GCGACATTTT TAAAAAATAT 
CTGTCGGTTA AAACACCTGT GCCAAATGGC TCACAACTCG CTGCTAAACC TACAGGGGAA 
ACGTCGGACT TCTCTGATGA GGATTCATAT GAAAGTAGCT TTCTATACAA CAGGAGTATG 
AGGAAGGCAT CAGGGTCCGG GAGAATTGAT CTAGATAATT TTACCAGATT AAGATGTGGT 
GTTGCGCCAT CAAATACCAA TGATAAGTCA GACAATGGGC AAAGTCAGGG GTTCTCCAAA 
CCCCCAACAG AAAACTGTGA AACGAAGAGT GAAAGAAACA CCGAGAGTAA ACGGTTAAAG 
GAAAGGTTGA TGTGGGCGCA TAAGTCTTTC TCTAGTATGT TTGATTTTAA GAATAATGAT 
AAAGATAGTG CCGCCCAGAG TTCAGAGGGC ACCCCTAGAG AGGAGAAGAA AAAGTCTAGG 
CCACTTCAGT CATCCTGGAA AACACTCCGA AATTCCAAAA ACAAAGATAA AGACTCATTT 
AAGAGGCTCA GCGTGTTGAA TTTATCAGCA CATATTATAA ATACAAACAT GCCAAGGGGG 
GCTTCAAAGG AAAATGGCAA TGAACATCTA CCCCCGATTC TTGATCCAAT AAAACAACCC 
TCCTCTATGG AAATAAAGCC TTGTATGACT ACTGATACAA ACACAGAGTC AGATTTGGAA 
CCTCTGGAAG CCAAGGAAAA CCATAAAAAA TGGAGCCTTG AAAATGATGA TTCTTTACAA 
ACTACTTGTG ATGACTCAGC TTTTTTTTCA CAATTTGACT CAAACGAATC ACATTCGTTA 
AACAAACAGC CCTCTATTAC AACTTTATTT AACCCATTCT CAAATTCTGA AATAAATCCC 
ATGCCATGTC GACCAATGAG TCCAAAGCCT TTTAGCCAAT GGCCCAGCTT TCAGCGTAGG 
GGCTTGCACA ATTCCAGGAT AAGTGCTACT TCGATGACCT CTCTAGCAAA CAGTTCTACA 
GTGGATGGGT GCCCAGATTC CCCAGAGACA GCAGTAAAAA CATGGGGAGG GTATTTAACA 
GACAGAAGTA GTCAGAAGGA TGACAGCAGT GCCAGCAGCC AATCAAAGCC CAGCCTTAAC 
ACTGCTTCTT CTGTAATGGA CATACTAAGA GATGAGGATA GTAGACAACT TGTCCCATCA 
TCGATACTAA AGATGCAAAG GGAAAGGAAT GCTTTGCTTT ACAAGAAGAG ACCTACAGAC 
CTGACGGCTT CTCAGTACCT GCCTATCAAC ACTGAAAGTA GAACACTGAC ACTGCCCCAG 
ATGGCTTCCA TGGGACACAA AAGGGATTCA GAACCAACAA GGCCTAAAGC TTTATTCAAG 
AGCCTGAGTT GTGATAACTT GTGGGTATAT GAGAGGAACC AAGAGAGGAA ACTAGACAAG 
TCTATAGATG CAGGAGATCT CATGAGGAAC CAGTCAAAAG CTTCTATGAG TGTCTCTATT 
GGGCCTCCAG AAATATTCCG AAGTTTCCCA ATGAAGGTGC ACTCGTTTTC GCGGAGCACA 
CCGAGCAGGC TTGATCTTGT GGGCTGTGTG AGGCGCACCT CTCTCTCAGT ATTCAATGAT 
GGGTCTACGA ACAGACCTTC GCTGTTTGAT GACTTGGGGA GTGATGAGGA TTTTTATGAA 
GAACTACACG TTTCTGGTCA CCGCTTTGTT GGAGGGGGCG AGCAGCTGGC AATAAATGAG 
TTGATAAGTG ATGGCAGTGT GGTATACGCC GAGGCTCTCT GGGACCATGT CACTATGGAT 
GATGAAGAAC TGGGTTTTAA GGCCGGAAGT GTCATCGAAG TAATGGATGC TACCAATAAA 
GAGTGGTGGT GGGGGAGGAT ACTTGATAGT GAAGGATGGT TTCCAGCCAG CTTTGTTAGG 
CTCCGTGTCA ACCAGGATGA ACCAATGGAA GACTGTGCTA CTAAAGCAGG GGACAAAGAG 
CAGGATTCTA GAAATGTCCC AAGGAGAAAC GGTTTTGGGC AGAACAACAA AGATCAAATG 
CGAACGAATG TTATTAATGA GATCTTAAAT ACAGAAAAAC ATTATATTAA GCACCTGAAG 
GATATTTGTG AGGGCTACAT CAAGCAGTGC CGTAAAAGGG ACGACATGTT TACAGAAGAC 
CAACTATGGA CAATCTTTGG AAACATTGAA GAGATCTACA AATTCCAAAA GAAATTCTTG 
AAAACACTTG AAAAAAGAAT TAACAAAGAT TCCCCGCATT TGAGTGAAAT CGGCTCTTGC 
TTTTTGGAAT ATCAAACAGA TTTCCAGATC TACTCAGAAT ATTGCAACAA TCATCCTAAT 
GCCTGCACAG AACTTTCCAA ACTCACCAAG GTCAAGAAGT ATGTCCATTT CTTTGAGACT 
TGCCGTTTAT TACAGAAGAT GATTGATATC TCTCTAGATG GGTTTCTTCT AACCCCGGTA 
CAGAAGATCT GTAAATATCC ACTTCAGCTG GCGGAACTAC TAAAGTACAC TAATTCCCAA 
CACAGAGACT ACACAAACGT TGAGGCAGCC TTAGATGCAA TGAAAAACGT TGCCAGACTT 
ATCAATGAAA GAAAGCGGAG ACTTGAGAAC ATTGATAAAA TAGCCCACTG GCAAAGTTCT 
ATAGAGGACT GGGAGGGAGA AGATATCCTT GCCAGAAGCT CGGAGCTCAT ACATTCTGGG 
GAACTAACCA AGATTTCTCA CCTCCAGCCA AAGGGACAGC AGCGGATATT CTTTCTGTTT 
GATCATCAAA TTGTTTTCTG CAAGAAGGAT CTGTTGCGAC GTGACATTCT GTATTATAAA 
GGGAAGATCG ACATGGACGA TATGGAGGTT CTGAATGTAG AGGATGGGAA AGACAAAGAC 
TTTAACATTA CTGTAAAAAA TGCATTCAAG CTGCAAGGCA AAGTCTCGGA AGAGATTCAC 
CTTTTCCTTG CAAAGAAACC AGAGCACAAA CAGCGATGGC TACAGGCCTT TGAGGAAGAA 
AGGAAACAGG TGCTATTGGA TAAAGAGACA GGTTTCAGCA TCAGTGAAAT ACAGAAGAAG 
CAGGCTATGA TTAATGCCAG TAAGCCTCAC CCGGCAGGAA AGCCTAAAGG TGTCAATAGG 
ACATACTATG ATTTCTGGAT GAAACAAAAG CACCCCACTC TGCCTGCCAA TCTCCCACAG 
CAACAAGTCT TCATGCTTGC CGAACCCAAG CGCAAACCCT CTAATTTCTG GCAGAATATC 
AGCCGACTGA CCCCGTTCAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef4 ( XM_002933352.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933398.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X2, mRNA.

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CloneID	OXa16059
Clone ID Related Accession (Same CDS sequence)	XM_002933352.4
Accession Version	XM_002933352.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933352.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGAAA TGGACATGAA GAATTCAAAA GAAAAGGTGG GTGGTATTTT ATCTGATTCT 
TTTCAAGACA TATTTAAAGG TTATTGGGAA GAGGACAGGC ACTCACGATC CAGTGAATCT 
GAAGAAAAAC AAAATATGAC ACTAGGCAGT ATTAAAAACA GCAGCCTGTT AAGGAAACAG 
GTTAGCAGAC AAGAGCCAAT GAGTACTGGC CATTTTGAAG ACTTTCAAGC TATTGGTGAC 
TCCTCAAGTA GCCTACAAGA CAGATCGCCT GTTATTGTAC TATCTGCCCA TCAGCCAAGA 
ACACAAACAG CATGGAAGGA AATTGAGCAG AATACATATA AACATGAAAA TGGCCATTCT 
TCACATTCAA ACAGCGATGA AAATTTAAGT GGTAGCAGAG AGTCTTTAAA CCAGGACCTA 
GTTTATAAAG CAAGATATTC AATGGGATGT GTGGAACAAG AAGAAGATGA GCAAAATGGC 
CCCACTTTCG TTGATATAAA CAGGGAAACT CCTCTCTTTC AAAAAGACTC AGAAACAATA 
TTTGTTCAAG AACATACAGA AGCCCATGGC AGGCACTTGT GGGACATATT GTTAGATAAA 
GAACAACACA ATCCCTCTCA AATAAGTATT CAAACAGCTA AAATAAAGTC CCTGAAGCAA 
AAAAGTTATA AGGATCCAAA CTACCATAGT AATGGTAATA TGGAGGATTC TAATCTTGAC 
CATGGTGACA GTGTCTTCTC TGCACAAAGA CGATATCTCA CAGCCCCACA AGATTCTGAT 
GGAGATGAAA GTGACTTTGA AGTTTCATCC TCGGAAGAAA ATAGAAATCA TTGTCTGGCA 
ACAGTTGAGG AGAGCAGAGA CGGCATGTGT TATTACAGTA CTATAAAGAA AATAGACACT 
ATAGACACAG TGGATGATAA TGAAAGCAAC TGCAGCAGCT CTAACAGTGA AGGCACTGAA 
GGCAACATGG GATACAACTG GAAAAGTGAG ATCAACAAAA TAGAAGACAT TAAAAGTGAC 
GTAAAGGCAT CAGAAAGCCA AAAAGACTCC AAAATAGCAG AATTAAAAGA TTCCAGAACA 
GAAATAAAAG AGGAGCCTAA CAATAAAATA AAACGGATGA ATATAAAAAA TAGCGACATT 
TTTAAAAAAT ATCTGTCGGT TAAAACACCT GTGCCAAATG GCTCACAACT CGCTGCTAAA 
CCTACAGGGG AAACGTCGGA CTTCTCTGAT GAGGATTCAT ATGAAAGTAG CTTTCTATAC 
AACAGGAGTA TGAGGAAGGC ATCAGGGTCC GGGAGAATTG ATCTAGATAA TTTTACCAGA 
TTAAGATGTG GTGTTGCGCC ATCAAATACC AATGATAAGT CAGACAATGG GCAAAGTCAG 
GGGTTCTCCA AACCCCCAAC AGAAAACTGT GAAACGAAGA GTGAAAGAAA CACCGAGAGT 
AAACGGTTAA AGGAAAGGTT GATGTGGGCG CATAAGTCTT TCTCTAGTAT GTTTGATTTT 
AAGAATAATG ATAAAGATAG TGCCGCCCAG AGTTCAGAGG GCACCCCTAG AGAGGAGAAG 
AAAAAGTCTA GGCCACTTCA GTCATCCTGG AAAACACTCC GAAATTCCAA AAACAAAGAT 
AAAGACTCAT TTAAGAGGCT CAGCGTGTTG AATTTATCAG CACATATTAT AAATACAAAC 
ATGCCAAGGG GGGCTTCAAA GGAAAATGGC AATGAACATC TACCCCCGAT TCTTGATCCA 
ATAAAACAAC CCTCCTCTAT GGAAATAAAG CCTTGTATGA CTACTGATAC AAACACAGAG 
TCAGATTTGG AACCTCTGGA AGCCAAGGAA AACCATAAAA AATGGAGCCT TGAAAATGAT 
GATTCTTTAC AAACTACTTG TGATGACTCA GCTTTTTTTT CACAATTTGA CTCAAACGAA 
TCACATTCGT TAAACAAACA GCCCTCTATT ACAACTTTAT TTAACCCATT CTCAAATTCT 
GAAATAAATC CCATGCCATG TCGACCAATG AGTCCAAAGC CTTTTAGCCA ATGGCCCAGC 
TTTCAGCGTA GGGGCTTGCA CAATTCCAGG ATAAGTGCTA CTTCGATGAC CTCTCTAGCA 
AACAGTTCTA CAGTGGATGG GTGCCCAGAT TCCCCAGAGA CAGCAGTAAA AACATGGGGA 
GGGTATTTAA CAGACAGAAG TAGTCAGAAG GATGACAGCA GTGCCAGCAG CCAATCAAAG 
CCCAGCCTTA ACACTGCTTC TTCTGTAATG GACATACTAA GAGATGAGGA TAGTAGACAA 
CTTGTCCCAT CATCGATACT AAAGATGCAA AGGGAAAGGA ATGCTTTGCT TTACAAGAAG 
AGACCTACAG ACCTGACGGC TTCTCAGTAC CTGCCTATCA ACACTGAAAG TAGAACACTG 
ACACTGCCCC AGATGGCTTC CATGGGACAC AAAAGGGATT CAGAACCAAC AAGGCCTAAA 
GCTTTATTCA AGAGCCTGAG TTGTGATAAC TTGTGGGTAT ATGAGAGGAA CCAAGAGAGG 
AAACTAGACA AGTCTATAGA TGCAGGAGAT CTCATGAGGA ACCAGTCAAA AGCTTCTATG 
AGTGTCTCTA TTGGGCCTCC AGAAATATTC CGAAGTTTCC CAATGAAGGT GCACTCGTTT 
TCGCGGAGCA CACCGAGCAG GCTTGATCTT GTGGGCTGTG TGAGGCGCAC CTCTCTCTCA 
GTATTCAATG ATGGGTCTAC GAACAGACCT TCGCTGTTTG ATGACTTGGG GAGTGATGAG 
GATTTTTATG AAGAACTACA CGTTTCTGGT CACCGCTTTG TTGGAGGGGG CGAGCAGCTG 
GCAATAAATG AGTTGATAAG TGATGGCAGT GTGGTATACG CCGAGGCTCT CTGGGACCAT 
GTCACTATGG ATGATGAAGA ACTGGGTTTT AAGGCCGGAA GTGTCATCGA AGTAATGGAT 
GCTACCAATA AAGAGTGGTG GTGGGGGAGG ATACTTGATA GTGAAGGATG GTTTCCAGCC 
AGCTTTGTTA GGCTCCGTGT CAACCAGGAT GAACCAATGG AAGACTGTGC TACTAAAGCA 
GGGGACAAAG AGCAGGATTC TAGAAATGTC CCAAGGAGAA ACGGTTTTGG GCAGAACAAC 
AAAGATCAAA TGCGAACGAA TGTTATTAAT GAGATCTTAA ATACAGAAAA ACATTATATT 
AAGCACCTGA AGGATATTTG TGAGGGCTAC ATCAAGCAGT GCCGTAAAAG GGACGACATG 
TTTACAGAAG ACCAACTATG GACAATCTTT GGAAACATTG AAGAGATCTA CAAATTCCAA 
AAGAAATTCT TGAAAACACT TGAAAAAAGA ATTAACAAAG ATTCCCCGCA TTTGAGTGAA 
ATCGGCTCTT GCTTTTTGGA ATATCAAACA GATTTCCAGA TCTACTCAGA ATATTGCAAC 
AATCATCCTA ATGCCTGCAC AGAACTTTCC AAACTCACCA AGGTCAAGAA GTATGTCCAT 
TTCTTTGAGA CTTGCCGTTT ATTACAGAAG ATGATTGATA TCTCTCTAGA TGGGTTTCTT 
CTAACCCCGG TACAGAAGAT CTGTAAATAT CCACTTCAGC TGGCGGAACT ACTAAAGTAC 
ACTAATTCCC AACACAGAGA CTACACAAAC GTTGAGGCAG CCTTAGATGC AATGAAAAAC 
GTTGCCAGAC TTATCAATGA AAGAAAGCGG AGACTTGAGA ACATTGATAA AATAGCCCAC 
TGGCAAAGTT CTATAGAGGA CTGGGAGGGA GAAGATATCC TTGCCAGAAG CTCGGAGCTC 
ATACATTCTG GGGAACTAAC CAAGATTTCT CACCTCCAGC CAAAGGGACA GCAGCGGATA 
TTCTTTCTGT TTGATCATCA AATTGTTTTC TGCAAGAAGG ATCTGTTGCG ACGTGACATT 
CTGTATTATA AAGGGAAGAT CGACATGGAC GATATGGAGG TTCTGAATGT AGAGGATGGG 
AAAGACAAAG ACTTTAACAT TACTGTAAAA AATGCATTCA AGCTGCAAGG CAAAGTCTCG 
GAAGAGATTC ACCTTTTCCT TGCAAAGAAA CCAGAGCACA AACAGCGATG GCTACAGGCC 
TTTGAGGAAG AAAGGAAACA GGTGCTATTG GATAAAGAGA CAGGTTTCAG CATCAGTGAA 
ATACAGAAGA AGCAGGCTAT GATTAATGCC AGTAAGCCTC ACCCGGCAGG AAAGCCTAAA 
GGTGTCAATA GGACATACTA TGATTTCTGG ATGAAACAAA AGCACCCCAC TCTGCCTGCC 
AATCTCCCAC AGCAACAAGT CTTCATGCTT GCCGAACCCA AGCGCAAACC CTCTAATTTC 
TGGCAGAATA TCAGCCGACT GACCCCGTTC AGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933398.2
CDS	1075..5433
Translation	MPEMDMKNSKEKVGGILSDSFQDIFKGYWEEDRHSRSSESEEKQNMTLGSIKNSSLLRKQVSRQEPMSTGHFEDFQAIGDSSSSLQDRSPVIVLSAHQPRTQTAWKEIEQNTYKHENGHSSHSNSDENLSGSRESLNQDLVYKARYSMGCVEQEEDEQNGPTFVDINRETPLFQKDSETIFVQEHTEAHGRHLWDILLDKEQHNPSQISIQTAKIKSLKQKSYKDPNYHSNGNMEDSNLDHGDSVFSAQRRYLTAPQDSDGDESDFEVSSSEENRNHCLATVEESRDGMCYYSTIKKIDTIDTVDDNESNCSSSNSEGTEGNMGYNWKSEINKIEDIKSDVKASESQKDSKIAELKDSRTEIKEEPNNKIKRMNIKNSDIFKKYLSVKTPVPNGSQLAAKPTGETSDFSDEDSYESSFLYNRSMRKASGSGRIDLDNFTRLRCGVAPSNTNDKSDNGQSQGFSKPPTENCETKSERNTESKRLKERLMWAHKSFSSMFDFKNNDKDSAAQSSEGTPREEKKKSRPLQSSWKTLRNSKNKDKDSFKRLSVLNLSAHIINTNMPRGASKENGNEHLPPILDPIKQPSSMEIKPCMTTDTNTESDLEPLEAKENHKKWSLENDDSLQTTCDDSAFFSQFDSNESHSLNKQPSITTLFNPFSNSEINPMPCRPMSPKPFSQWPSFQRRGLHNSRISATSMTSLANSSTVDGCPDSPETAVKTWGGYLTDRSSQKDDSSASSQSKPSLNTASSVMDILRDEDSRQLVPSSILKMQRERNALLYKKRPTDLTASQYLPINTESRTLTLPQMASMGHKRDSEPTRPKALFKSLSCDNLWVYERNQERKLDKSIDAGDLMRNQSKASMSVSIGPPEIFRSFPMKVHSFSRSTPSRLDLVGCVRRTSLSVFNDGSTNRPSLFDDLGSDEDFYEELHVSGHRFVGGGEQLAINELISDGSVVYAEALWDHVTMDDEELGFKAGSVIEVMDATNKEWWWGRILDSEGWFPASFVRLRVNQDEPMEDCATKAGDKEQDSRNVPRRNGFGQNNKDQMRTNVINEILNTEKHYIKHLKDICEGYIKQCRKRDDMFTEDQLWTIFGNIEEIYKFQKKFLKTLEKRINKDSPHLSEIGSCFLEYQTDFQIYSEYCNNHPNACTELSKLTKVKKYVHFFETCRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNSQHRDYTNVEAALDAMKNVARLINERKRRLENIDKIAHWQSSIEDWEGEDILARSSELIHSGELTKISHLQPKGQQRIFFLFDHQIVFCKKDLLRRDILYYKGKIDMDDMEVLNVEDGKDKDFNITVKNAFKLQGKVSEEIHLFLAKKPEHKQRWLQAFEEERKQVLLDKETGFSISEIQKKQAMINASKPHPAGKPKGVNRTYYDFWMKQKHPTLPANLPQQQVFMLAEPKRKPSNFWQNISRLTPFRK

Target ORF information:

RefSeq Version	XM_002933352.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 4 (arhgef4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933352.4

ORF Insert Sequence:

ATGCCAGAAA TGGACATGAA GAATTCAAAA GAAAAGGTGG GTGGTATTTT ATCTGATTCT 
TTTCAAGACA TATTTAAAGG TTATTGGGAA GAGGACAGGC ACTCACGATC CAGTGAATCT 
GAAGAAAAAC AAAATATGAC ACTAGGCAGT ATTAAAAACA GCAGCCTGTT AAGGAAACAG 
GTTAGCAGAC AAGAGCCAAT GAGTACTGGC CATTTTGAAG ACTTTCAAGC TATTGGTGAC 
TCCTCAAGTA GCCTACAAGA CAGATCGCCT GTTATTGTAC TATCTGCCCA TCAGCCAAGA 
ACACAAACAG CATGGAAGGA AATTGAGCAG AATACATATA AACATGAAAA TGGCCATTCT 
TCACATTCAA ACAGCGATGA AAATTTAAGT GGTAGCAGAG AGTCTTTAAA CCAGGACCTA 
GTTTATAAAG CAAGATATTC AATGGGATGT GTGGAACAAG AAGAAGATGA GCAAAATGGC 
CCCACTTTCG TTGATATAAA CAGGGAAACT CCTCTCTTTC AAAAAGACTC AGAAACAATA 
TTTGTTCAAG AACATACAGA AGCCCATGGC AGGCACTTGT GGGACATATT GTTAGATAAA 
GAACAACACA ATCCCTCTCA AATAAGTATT CAAACAGCTA AAATAAAGTC CCTGAAGCAA 
AAAAGTTATA AGGATCCAAA CTACCATAGT AATGGTAATA TGGAGGATTC TAATCTTGAC 
CATGGTGACA GTGTCTTCTC TGCACAAAGA CGATATCTCA CAGCCCCACA AGATTCTGAT 
GGAGATGAAA GTGACTTTGA AGTTTCATCC TCGGAAGAAA ATAGAAATCA TTGTCTGGCA 
ACAGTTGAGG AGAGCAGAGA CGGCATGTGT TATTACAGTA CTATAAAGAA AATAGACACT 
ATAGACACAG TGGATGATAA TGAAAGCAAC TGCAGCAGCT CTAACAGTGA AGGCACTGAA 
GGCAACATGG GATACAACTG GAAAAGTGAG ATCAACAAAA TAGAAGACAT TAAAAGTGAC 
GTAAAGGCAT CAGAAAGCCA AAAAGACTCC AAAATAGCAG AATTAAAAGA TTCCAGAACA 
GAAATAAAAG AGGAGCCTAA CAATAAAATA AAACGGATGA ATATAAAAAA TAGCGACATT 
TTTAAAAAAT ATCTGTCGGT TAAAACACCT GTGCCAAATG GCTCACAACT CGCTGCTAAA 
CCTACAGGGG AAACGTCGGA CTTCTCTGAT GAGGATTCAT ATGAAAGTAG CTTTCTATAC 
AACAGGAGTA TGAGGAAGGC ATCAGGGTCC GGGAGAATTG ATCTAGATAA TTTTACCAGA 
TTAAGATGTG GTGTTGCGCC ATCAAATACC AATGATAAGT CAGACAATGG GCAAAGTCAG 
GGGTTCTCCA AACCCCCAAC AGAAAACTGT GAAACGAAGA GTGAAAGAAA CACCGAGAGT 
AAACGGTTAA AGGAAAGGTT GATGTGGGCG CATAAGTCTT TCTCTAGTAT GTTTGATTTT 
AAGAATAATG ATAAAGATAG TGCCGCCCAG AGTTCAGAGG GCACCCCTAG AGAGGAGAAG 
AAAAAGTCTA GGCCACTTCA GTCATCCTGG AAAACACTCC GAAATTCCAA AAACAAAGAT 
AAAGACTCAT TTAAGAGGCT CAGCGTGTTG AATTTATCAG CACATATTAT AAATACAAAC 
ATGCCAAGGG GGGCTTCAAA GGAAAATGGC AATGAACATC TACCCCCGAT TCTTGATCCA 
ATAAAACAAC CCTCCTCTAT GGAAATAAAG CCTTGTATGA CTACTGATAC AAACACAGAG 
TCAGATTTGG AACCTCTGGA AGCCAAGGAA AACCATAAAA AATGGAGCCT TGAAAATGAT 
GATTCTTTAC AAACTACTTG TGATGACTCA GCTTTTTTTT CACAATTTGA CTCAAACGAA 
TCACATTCGT TAAACAAACA GCCCTCTATT ACAACTTTAT TTAACCCATT CTCAAATTCT 
GAAATAAATC CCATGCCATG TCGACCAATG AGTCCAAAGC CTTTTAGCCA ATGGCCCAGC 
TTTCAGCGTA GGGGCTTGCA CAATTCCAGG ATAAGTGCTA CTTCGATGAC CTCTCTAGCA 
AACAGTTCTA CAGTGGATGG GTGCCCAGAT TCCCCAGAGA CAGCAGTAAA AACATGGGGA 
GGGTATTTAA CAGACAGAAG TAGTCAGAAG GATGACAGCA GTGCCAGCAG CCAATCAAAG 
CCCAGCCTTA ACACTGCTTC TTCTGTAATG GACATACTAA GAGATGAGGA TAGTAGACAA 
CTTGTCCCAT CATCGATACT AAAGATGCAA AGGGAAAGGA ATGCTTTGCT TTACAAGAAG 
AGACCTACAG ACCTGACGGC TTCTCAGTAC CTGCCTATCA ACACTGAAAG TAGAACACTG 
ACACTGCCCC AGATGGCTTC CATGGGACAC AAAAGGGATT CAGAACCAAC AAGGCCTAAA 
GCTTTATTCA AGAGCCTGAG TTGTGATAAC TTGTGGGTAT ATGAGAGGAA CCAAGAGAGG 
AAACTAGACA AGTCTATAGA TGCAGGAGAT CTCATGAGGA ACCAGTCAAA AGCTTCTATG 
AGTGTCTCTA TTGGGCCTCC AGAAATATTC CGAAGTTTCC CAATGAAGGT GCACTCGTTT 
TCGCGGAGCA CACCGAGCAG GCTTGATCTT GTGGGCTGTG TGAGGCGCAC CTCTCTCTCA 
GTATTCAATG ATGGGTCTAC GAACAGACCT TCGCTGTTTG ATGACTTGGG GAGTGATGAG 
GATTTTTATG AAGAACTACA CGTTTCTGGT CACCGCTTTG TTGGAGGGGG CGAGCAGCTG 
GCAATAAATG AGTTGATAAG TGATGGCAGT GTGGTATACG CCGAGGCTCT CTGGGACCAT 
GTCACTATGG ATGATGAAGA ACTGGGTTTT AAGGCCGGAA GTGTCATCGA AGTAATGGAT 
GCTACCAATA AAGAGTGGTG GTGGGGGAGG ATACTTGATA GTGAAGGATG GTTTCCAGCC 
AGCTTTGTTA GGCTCCGTGT CAACCAGGAT GAACCAATGG AAGACTGTGC TACTAAAGCA 
GGGGACAAAG AGCAGGATTC TAGAAATGTC CCAAGGAGAA ACGGTTTTGG GCAGAACAAC 
AAAGATCAAA TGCGAACGAA TGTTATTAAT GAGATCTTAA ATACAGAAAA ACATTATATT 
AAGCACCTGA AGGATATTTG TGAGGGCTAC ATCAAGCAGT GCCGTAAAAG GGACGACATG 
TTTACAGAAG ACCAACTATG GACAATCTTT GGAAACATTG AAGAGATCTA CAAATTCCAA 
AAGAAATTCT TGAAAACACT TGAAAAAAGA ATTAACAAAG ATTCCCCGCA TTTGAGTGAA 
ATCGGCTCTT GCTTTTTGGA ATATCAAACA GATTTCCAGA TCTACTCAGA ATATTGCAAC 
AATCATCCTA ATGCCTGCAC AGAACTTTCC AAACTCACCA AGGTCAAGAA GTATGTCCAT 
TTCTTTGAGA CTTGCCGTTT ATTACAGAAG ATGATTGATA TCTCTCTAGA TGGGTTTCTT 
CTAACCCCGG TACAGAAGAT CTGTAAATAT CCACTTCAGC TGGCGGAACT ACTAAAGTAC 
ACTAATTCCC AACACAGAGA CTACACAAAC GTTGAGGCAG CCTTAGATGC AATGAAAAAC 
GTTGCCAGAC TTATCAATGA AAGAAAGCGG AGACTTGAGA ACATTGATAA AATAGCCCAC 
TGGCAAAGTT CTATAGAGGA CTGGGAGGGA GAAGATATCC TTGCCAGAAG CTCGGAGCTC 
ATACATTCTG GGGAACTAAC CAAGATTTCT CACCTCCAGC CAAAGGGACA GCAGCGGATA 
TTCTTTCTGT TTGATCATCA AATTGTTTTC TGCAAGAAGG ATCTGTTGCG ACGTGACATT 
CTGTATTATA AAGGGAAGAT CGACATGGAC GATATGGAGG TTCTGAATGT AGAGGATGGG 
AAAGACAAAG ACTTTAACAT TACTGTAAAA AATGCATTCA AGCTGCAAGG CAAAGTCTCG 
GAAGAGATTC ACCTTTTCCT TGCAAAGAAA CCAGAGCACA AACAGCGATG GCTACAGGCC 
TTTGAGGAAG AAAGGAAACA GGTGCTATTG GATAAAGAGA CAGGTTTCAG CATCAGTGAA 
ATACAGAAGA AGCAGGCTAT GATTAATGCC AGTAAGCCTC ACCCGGCAGG AAAGCCTAAA 
GGTGTCAATA GGACATACTA TGATTTCTGG ATGAAACAAA AGCACCCCAC TCTGCCTGCC 
AATCTCCCAC AGCAACAAGT CTTCATGCTT GCCGAACCCA AGCGCAAACC CTCTAATTTC 
TGGCAGAATA TCAGCCGACT GACCCCGTTC AGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

arhgef4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

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