Arhgef4 cDNA ORF clone, Mus musculus(house mouse)

The following Arhgef4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgef4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu08296 NM_183019.3
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $286.30
$409.00
OMu85142 XM_030253614.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu85139 XM_030253609.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu85140 XM_030253611.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu85141 XM_030253613.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu84554 NM_001368773.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu85135 XM_030253601.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu85136 XM_030253603.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu85137 XM_030253604.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu85138 XM_030253606.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
View Old Accession Versions >>
OMu08296 NM_183019.2 Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $286.30
$409.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu08296
    Clone ID Related Accession (Same CDS sequence) NM_183019.3 , NM_183019.2
    Accession Version NM_183019.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 4 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107738.16. On Feb 25, 2019 this sequence version replaced NM_183019.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.188481.1, SRR7345562.746938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGACC AGGAGCTGGG CTTCAAAGCT GGGGATGTCA TCGAAGTGAT GGATGCCACC 
    AACAGGGAAT GGTGGTGGGG CCGAGTGGCA GATGGCGAGG GGTGGTTCCC TGCCAGCTTT
    GTACGGCTGC GCGTGAACCA GGATGAGCCC GCGGATGACT ATGAGGCCCC GCGGGCCGGG
    GCTAGAGAGG CCGATGACAG CGGTCCAGAG GCTCAGAGCT GCAAGGACCA GATGCGCACA
    AATGTCATCA ATGAGATCCT CAGCACTGAG CGAGACTACA TCAAGCACCT TCGCGACATC
    TGCGAGGGCT ATGTCCGCCA GTGCCGCAAA CGGGAGGATA TGTTCAGTGA AGAGCAGCTG
    CGCACCATCT TTGGGAACAT CGAGGACATC TACCGCTGCC AAAAGGCATT TGTGAAGGCT
    TTGGAGCAGA AGTTCAACAC GGAGCGGCCA CACCTGAGCG AGTTGGGGGC CTGCTTTCTG
    GAACACCAAG CAGACTTCCA GATCTATTCT GAGTACTGCA ACAACCACCC CAATGCCTGC
    GTGGAGCTCT CCCGGCTCAC GAAGCTCAGC AAGTACGTGT ACTTCTTCGA GGCCTGCCGG
    CTGCTGCAAA GGATGATTGA CATCTCGCTG GATGGCTTTC TGCTGACCCC GGTGCAGAAG
    ATCTGCAAGT ATCCCCTCCA GCTGGCGGAG CTGCTCAAGT ACACACACCC CCAGCACAGG
    GACTTTAAGA ATGTTGAAGC TGCCTTGCAC GCCATGAAGA ATGTGGCCCA GCTCATCAAC
    GAACGGAAAC GAAGACTTGA AAACATCGAC AAGATTGCTC AGTGGCAGAG CTCCATAGAG
    GACTGGGAGG GGGAAGACCT TCTGGTCAGG AGCTCGGAAC TCATCCACTC GGGGGAGCTG
    ACCCGCGTGA CACAGCCTCA AGCCAGGAGT CAGCAGAGAA TGTTTTTTCT CTTTGACCGC
    CAGCTCATCT ACTGTAAAAA GGATCTGCTT CGCCGGGACG TGCTGTACTA CAAGGGCCGG
    CTGGACATGG ATGACCTGGA GGTGGTAGAT GTGGAAGATG GGAAGGACCG AGACCTCCAT
    GTGAGTGTCA AGAACGCCTT CCGTCTGTAC TGTGGTACCA CGGGGGACAG CCACCTACTG
    TGTGCCAGGA AGCCAGAACA GAAGCAGCGC TGGCTGAAGG CCTTTGCCAG AGAGAGGGAG
    CAGGTGCGGC TGGACCAGGA GACAGGCTTC TCCATCACCG AGCTGCAGAG GAAGCAGGCC
    ATGCTGAACG CCAGCAAGCA GCAGGCCACG GGGAAGCCTA AAGCTGTGGG AAGGCCAGGA
    TACCTGACCC GCCACAAGCA TCCATCATTG CCTGCCAGCC GGCCCCAGCA GCAAGTGTTG
    GTGCTAGCAG AGCCCAGGCG GAAGCCATCT AACTTCTGGC ACAGCATCAG CAGACTGGCG
    CCTTTCCGCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_898840.2
    CDS316..1770
    Translation

    Target ORF information:

    RefSeq Version NM_183019.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_183019.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGACC AGGAGCTGGG CTTCAAAGCT GGGGATGTCA TCGAAGTGAT GGATGCCACC 
    AACAGGGAAT GGTGGTGGGG CCGAGTGGCA GATGGCGAGG GGTGGTTCCC TGCCAGCTTT
    GTACGGCTGC GCGTGAACCA GGATGAGCCC GCGGATGACT ATGAGGCCCC GCGGGCCGGG
    GCTAGAGAGG CCGATGACAG CGGTCCAGAG GCTCAGAGCT GCAAGGACCA GATGCGCACA
    AATGTCATCA ATGAGATCCT CAGCACTGAG CGAGACTACA TCAAGCACCT TCGCGACATC
    TGCGAGGGCT ATGTCCGCCA GTGCCGCAAA CGGGAGGATA TGTTCAGTGA AGAGCAGCTG
    CGCACCATCT TTGGGAACAT CGAGGACATC TACCGCTGCC AAAAGGCATT TGTGAAGGCT
    TTGGAGCAGA AGTTCAACAC GGAGCGGCCA CACCTGAGCG AGTTGGGGGC CTGCTTTCTG
    GAACACCAAG CAGACTTCCA GATCTATTCT GAGTACTGCA ACAACCACCC CAATGCCTGC
    GTGGAGCTCT CCCGGCTCAC GAAGCTCAGC AAGTACGTGT ACTTCTTCGA GGCCTGCCGG
    CTGCTGCAAA GGATGATTGA CATCTCGCTG GATGGCTTTC TGCTGACCCC GGTGCAGAAG
    ATCTGCAAGT ATCCCCTCCA GCTGGCGGAG CTGCTCAAGT ACACACACCC CCAGCACAGG
    GACTTTAAGA ATGTTGAAGC TGCCTTGCAC GCCATGAAGA ATGTGGCCCA GCTCATCAAC
    GAACGGAAAC GAAGACTTGA AAACATCGAC AAGATTGCTC AGTGGCAGAG CTCCATAGAG
    GACTGGGAGG GGGAAGACCT TCTGGTCAGG AGCTCGGAAC TCATCCACTC GGGGGAGCTG
    ACCCGCGTGA CACAGCCTCA AGCCAGGAGT CAGCAGAGAA TGTTTTTTCT CTTTGACCGC
    CAGCTCATCT ACTGTAAAAA GGATCTGCTT CGCCGGGACG TGCTGTACTA CAAGGGCCGG
    CTGGACATGG ATGACCTGGA GGTGGTAGAT GTGGAAGATG GGAAGGACCG AGACCTCCAT
    GTGAGTGTCA AGAACGCCTT CCGTCTGTAC TGTGGTACCA CGGGGGACAG CCACCTACTG
    TGTGCCAGGA AGCCAGAACA GAAGCAGCGC TGGCTGAAGG CCTTTGCCAG AGAGAGGGAG
    CAGGTGCGGC TGGACCAGGA GACAGGCTTC TCCATCACCG AGCTGCAGAG GAAGCAGGCC
    ATGCTGAACG CCAGCAAGCA GCAGGCCACG GGGAAGCCTA AAGCTGTGGG AAGGCCAGGA
    TACCTGACCC GCCACAAGCA TCCATCATTG CCTGCCAGCC GGCCCCAGCA GCAAGTGTTG
    GTGCTAGCAG AGCCCAGGCG GAAGCCATCT AACTTCTGGC ACAGCATCAG CAGACTGGCG
    CCTTTCCGCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu08296
    Clone ID Related Accession (Same CDS sequence) NM_183019.3 , NM_183019.2
    Accession Version NM_183019.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-22
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB609907.1, BC055804.1 and AK034013.1. On Dec 17, 2004 this sequence version replaced NM_183019.1. ##Evidence-Data-START## Transcript exon combination :: AK169957.1, AK163424.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGACC AGGAGCTGGG CTTCAAAGCT GGGGATGTCA TCGAAGTGAT GGATGCCACC 
    AACAGGGAAT GGTGGTGGGG CCGAGTGGCA GATGGCGAGG GGTGGTTCCC TGCCAGCTTT
    GTACGGCTGC GCGTGAACCA GGATGAGCCC GCGGATGACT ATGAGGCCCC GCGGGCCGGG
    GCTAGAGAGG CCGATGACAG CGGTCCAGAG GCTCAGAGCT GCAAGGACCA GATGCGCACA
    AATGTCATCA ATGAGATCCT CAGCACTGAG CGAGACTACA TCAAGCACCT TCGCGACATC
    TGCGAGGGCT ATGTCCGCCA GTGCCGCAAA CGGGAGGATA TGTTCAGTGA AGAGCAGCTG
    CGCACCATCT TTGGGAACAT CGAGGACATC TACCGCTGCC AAAAGGCATT TGTGAAGGCT
    TTGGAGCAGA AGTTCAACAC GGAGCGGCCA CACCTGAGCG AGTTGGGGGC CTGCTTTCTG
    GAACACCAAG CAGACTTCCA GATCTATTCT GAGTACTGCA ACAACCACCC CAATGCCTGC
    GTGGAGCTCT CCCGGCTCAC GAAGCTCAGC AAGTACGTGT ACTTCTTCGA GGCCTGCCGG
    CTGCTGCAAA GGATGATTGA CATCTCGCTG GATGGCTTTC TGCTGACCCC GGTGCAGAAG
    ATCTGCAAGT ATCCCCTCCA GCTGGCGGAG CTGCTCAAGT ACACACACCC CCAGCACAGG
    GACTTTAAGA ATGTTGAAGC TGCCTTGCAC GCCATGAAGA ATGTGGCCCA GCTCATCAAC
    GAACGGAAAC GAAGACTTGA AAACATCGAC AAGATTGCTC AGTGGCAGAG CTCCATAGAG
    GACTGGGAGG GGGAAGACCT TCTGGTCAGG AGCTCGGAAC TCATCCACTC GGGGGAGCTG
    ACCCGCGTGA CACAGCCTCA AGCCAGGAGT CAGCAGAGAA TGTTTTTTCT CTTTGACCGC
    CAGCTCATCT ACTGTAAAAA GGATCTGCTT CGCCGGGACG TGCTGTACTA CAAGGGCCGG
    CTGGACATGG ATGACCTGGA GGTGGTAGAT GTGGAAGATG GGAAGGACCG AGACCTCCAT
    GTGAGTGTCA AGAACGCCTT CCGTCTGTAC TGTGGTACCA CGGGGGACAG CCACCTACTG
    TGTGCCAGGA AGCCAGAACA GAAGCAGCGC TGGCTGAAGG CCTTTGCCAG AGAGAGGGAG
    CAGGTGCGGC TGGACCAGGA GACAGGCTTC TCCATCACCG AGCTGCAGAG GAAGCAGGCC
    ATGCTGAACG CCAGCAAGCA GCAGGCCACG GGGAAGCCTA AAGCTGTGGG AAGGCCAGGA
    TACCTGACCC GCCACAAGCA TCCATCATTG CCTGCCAGCC GGCCCCAGCA GCAAGTGTTG
    GTGCTAGCAG AGCCCAGGCG GAAGCCATCT AACTTCTGGC ACAGCATCAG CAGACTGGCG
    CCTTTCCGCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_898840.2
    CDS335..1789
    Translation

    Target ORF information:

    RefSeq Version NM_183019.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_183019.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGACC AGGAGCTGGG CTTCAAAGCT GGGGATGTCA TCGAAGTGAT GGATGCCACC 
    AACAGGGAAT GGTGGTGGGG CCGAGTGGCA GATGGCGAGG GGTGGTTCCC TGCCAGCTTT
    GTACGGCTGC GCGTGAACCA GGATGAGCCC GCGGATGACT ATGAGGCCCC GCGGGCCGGG
    GCTAGAGAGG CCGATGACAG CGGTCCAGAG GCTCAGAGCT GCAAGGACCA GATGCGCACA
    AATGTCATCA ATGAGATCCT CAGCACTGAG CGAGACTACA TCAAGCACCT TCGCGACATC
    TGCGAGGGCT ATGTCCGCCA GTGCCGCAAA CGGGAGGATA TGTTCAGTGA AGAGCAGCTG
    CGCACCATCT TTGGGAACAT CGAGGACATC TACCGCTGCC AAAAGGCATT TGTGAAGGCT
    TTGGAGCAGA AGTTCAACAC GGAGCGGCCA CACCTGAGCG AGTTGGGGGC CTGCTTTCTG
    GAACACCAAG CAGACTTCCA GATCTATTCT GAGTACTGCA ACAACCACCC CAATGCCTGC
    GTGGAGCTCT CCCGGCTCAC GAAGCTCAGC AAGTACGTGT ACTTCTTCGA GGCCTGCCGG
    CTGCTGCAAA GGATGATTGA CATCTCGCTG GATGGCTTTC TGCTGACCCC GGTGCAGAAG
    ATCTGCAAGT ATCCCCTCCA GCTGGCGGAG CTGCTCAAGT ACACACACCC CCAGCACAGG
    GACTTTAAGA ATGTTGAAGC TGCCTTGCAC GCCATGAAGA ATGTGGCCCA GCTCATCAAC
    GAACGGAAAC GAAGACTTGA AAACATCGAC AAGATTGCTC AGTGGCAGAG CTCCATAGAG
    GACTGGGAGG GGGAAGACCT TCTGGTCAGG AGCTCGGAAC TCATCCACTC GGGGGAGCTG
    ACCCGCGTGA CACAGCCTCA AGCCAGGAGT CAGCAGAGAA TGTTTTTTCT CTTTGACCGC
    CAGCTCATCT ACTGTAAAAA GGATCTGCTT CGCCGGGACG TGCTGTACTA CAAGGGCCGG
    CTGGACATGG ATGACCTGGA GGTGGTAGAT GTGGAAGATG GGAAGGACCG AGACCTCCAT
    GTGAGTGTCA AGAACGCCTT CCGTCTGTAC TGTGGTACCA CGGGGGACAG CCACCTACTG
    TGTGCCAGGA AGCCAGAACA GAAGCAGCGC TGGCTGAAGG CCTTTGCCAG AGAGAGGGAG
    CAGGTGCGGC TGGACCAGGA GACAGGCTTC TCCATCACCG AGCTGCAGAG GAAGCAGGCC
    ATGCTGAACG CCAGCAAGCA GCAGGCCACG GGGAAGCCTA AAGCTGTGGG AAGGCCAGGA
    TACCTGACCC GCCACAAGCA TCCATCATTG CCTGCCAGCC GGCCCCAGCA GCAAGTGTTG
    GTGCTAGCAG AGCCCAGGCG GAAGCCATCT AACTTCTGGC ACAGCATCAG CAGACTGGCG
    CCTTTCCGCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85142
    Clone ID Related Accession (Same CDS sequence) XM_030253614.1
    Accession Version XM_030253614.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1245bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGATGACC AGGAGCTGGG CTTCAAAGCT GGGGATGTCA TCGAAGTGAT GGATGCCACC 
    AACAGGGAAT GGTGGTGGGG CCGAGTGGCA GATGGCGAGG GGTGGTTCCC TGCCAGCTTT
    GTACGGCTGC GCGTGAACCA GGATGAGCCC GCGGATGACT ATGAGGCCCC GCGGGCCGGG
    GCTAGAGAGG CCGATGACAG CGGTCCAGAG GCTCAGAGCT GCAAGGACCA GATGCGCACA
    AATGTCATCA ATGAGATCCT CAGCACTGAG CGAGACTACA TCAAGCACCT TCGCGACATC
    TGCGAGGGCT ATGTCCGCCA GTGCCGCAAA CGGGAGGATA TGTTCAGTGA AGAGCAGCTG
    CGCACCATCT TTGGGAACAT CGAGGACATC TACCGCTGCC AAAAGGCATT TGTGAAGGCT
    TTGGAGCAGA AGTTCAACAC GGAGCGGCCA CACCTGAGCG AGTTGGGGGC CTGCTTTCTG
    GAACACCAAG CAGACTTCCA GATCTATTCT GAGTACTGCA ACAACCACCC CAATGCCTGC
    GTGGAGCTCT CCCGGCTCAC GAAGCTCAGC AAGTACGTGT ACTTCTTCGA GGCCTGCCGG
    CTGCTGCAAA GGATGATTGA CATCTCGCTG GATGGCTTTC TGCTGACCCC GGTGCAGAAG
    ATCTGCAAGT ATCCCCTCCA GCTGGCGGAG CTGCTCAAGT ACACACACCC CCAGCACAGG
    GACTTTAAGA ATGTTGAAGC TGCCTTGCAC GCCATGAAGA ATGTGGCCCA GCTCATCAAC
    GAACGGAAAC GAAGACTTGA AAACATCGAC AAGATTGCTC AGTGGCAGAG CTCCATAGAG
    GACTGGGAGG GGGAAGACCT TCTGGTCAGG AGCTCGGAAC TCATCCACTC GGGGGAGCTG
    ACCCGCGTGA CACAGCCTCA AGCCAGGAGT CAGCAGAGAA TGTTTTTTCT CTTTGACCGC
    CAGCTCATCT ACTGTAAAAA GGATCTGCTT CGCCGGGACG TGCTGTACTA CAAGGGCCGG
    CTGGACATGG ATGACCTGGA GGTGGTAGAT GTGGAAGATG GGAAGGACCG AGACCTCCAT
    GTGAGTGTCA AGAACGCCTT CCGTCTGTAC TGTGGTACCA CGGGGGACAG CCACCTACTG
    TGTGCCAGGA AGCCAGAACA GAAGCAGCGC TGGCTGAAGG CCTTTGCCAG AGAGAGGGAG
    CAGGCTTCTC CATCACCGAG CTGCAGAGGA AGCAGGCCAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109474.1
    CDS394..1638
    Translation

    Target ORF information:

    RefSeq Version XM_030253614.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253614.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGATGACC AGGAGCTGGG CTTCAAAGCT GGGGATGTCA TCGAAGTGAT GGATGCCACC 
    AACAGGGAAT GGTGGTGGGG CCGAGTGGCA GATGGCGAGG GGTGGTTCCC TGCCAGCTTT
    GTACGGCTGC GCGTGAACCA GGATGAGCCC GCGGATGACT ATGAGGCCCC GCGGGCCGGG
    GCTAGAGAGG CCGATGACAG CGGTCCAGAG GCTCAGAGCT GCAAGGACCA GATGCGCACA
    AATGTCATCA ATGAGATCCT CAGCACTGAG CGAGACTACA TCAAGCACCT TCGCGACATC
    TGCGAGGGCT ATGTCCGCCA GTGCCGCAAA CGGGAGGATA TGTTCAGTGA AGAGCAGCTG
    CGCACCATCT TTGGGAACAT CGAGGACATC TACCGCTGCC AAAAGGCATT TGTGAAGGCT
    TTGGAGCAGA AGTTCAACAC GGAGCGGCCA CACCTGAGCG AGTTGGGGGC CTGCTTTCTG
    GAACACCAAG CAGACTTCCA GATCTATTCT GAGTACTGCA ACAACCACCC CAATGCCTGC
    GTGGAGCTCT CCCGGCTCAC GAAGCTCAGC AAGTACGTGT ACTTCTTCGA GGCCTGCCGG
    CTGCTGCAAA GGATGATTGA CATCTCGCTG GATGGCTTTC TGCTGACCCC GGTGCAGAAG
    ATCTGCAAGT ATCCCCTCCA GCTGGCGGAG CTGCTCAAGT ACACACACCC CCAGCACAGG
    GACTTTAAGA ATGTTGAAGC TGCCTTGCAC GCCATGAAGA ATGTGGCCCA GCTCATCAAC
    GAACGGAAAC GAAGACTTGA AAACATCGAC AAGATTGCTC AGTGGCAGAG CTCCATAGAG
    GACTGGGAGG GGGAAGACCT TCTGGTCAGG AGCTCGGAAC TCATCCACTC GGGGGAGCTG
    ACCCGCGTGA CACAGCCTCA AGCCAGGAGT CAGCAGAGAA TGTTTTTTCT CTTTGACCGC
    CAGCTCATCT ACTGTAAAAA GGATCTGCTT CGCCGGGACG TGCTGTACTA CAAGGGCCGG
    CTGGACATGG ATGACCTGGA GGTGGTAGAT GTGGAAGATG GGAAGGACCG AGACCTCCAT
    GTGAGTGTCA AGAACGCCTT CCGTCTGTAC TGTGGTACCA CGGGGGACAG CCACCTACTG
    TGTGCCAGGA AGCCAGAACA GAAGCAGCGC TGGCTGAAGG CCTTTGCCAG AGAGAGGGAG
    CAGGCTTCTC CATCACCGAG CTGCAGAGGA AGCAGGCCAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85139
    Clone ID Related Accession (Same CDS sequence) XM_030253609.1
    Accession Version XM_030253609.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGGGGG ACCCGAAGCT ACGGCAGTGC GGCGGGGATG CTGCTGGCGA CCCCGCCGGC 
    CTGTGGCTGG AGCTCGGAGC CGCTTGCCCC GGGGCGCCGC GCTGCCCCAG CGAGTCCAGT
    GGCCCTACCA GCGGCGATCT GGGCACAAGC AGCAGCAGCA GCACTGGGGT GTCCCCGGGT
    TCCGACTCGG ACAGCAGCGG CGTGGTGTGC AGTGGCCGCG CTGCGGGCAT GCGCGGCGCC
    CCGTCGCGCT CCCGGAGCCT GGAGAGCCTG CGCTCGGCCT CGGCGGGGAT GCCTGACGGA
    ACTCTGGACA CGGTGTGCGC CGAGGAGACA GGCAGTGAGG AGGACCTGTA TGAAGAGCTT
    CACAACTCTG GCCACCACTA CAGCCACCCT AGAGGAGGTG GAGAGCAACT GGCCATCAAT
    GAGCTCATCA GTGATGGCAG TGTGGTGTGT GCTGAAGCCC TGTGGGACCA TGTCACCATG
    GATGACCAGG AGCTGGGCTT CAAAGCTGGG GATGTCATCG AAGTGATGGA TGCCACCAAC
    AGGGAATGGT GGTGGGGCCG AGTGGCAGAT GGCGAGGGGT GGTTCCCTGC CAGCTTTGTA
    CGGCTGCGCG TGAACCAGGA TGAGCCCGCG GATGACTATG AGGCCCCGCG GGCCGGGGCT
    AGAGAGGCCG ATGACAGCGG TCCAGAGGCT CAGAGCTGCA AGGACCAGAT GCGCACAAAT
    GTCATCAATG AGATCCTCAG CACTGAGCGA GACTACATCA AGCACCTTCG CGACATCTGC
    GAGGGCTATG TCCGCCAGTG CCGCAAACGG GAGGATATGT TCAGTGAAGA GCAGCTGCGC
    ACCATCTTTG GGAACATCGA GGACATCTAC CGCTGCCAAA AGGCATTTGT GAAGGCTTTG
    GAGCAGAAGT TCAACACGGA GCGGCCACAC CTGAGCGAGT TGGGGGCCTG CTTTCTGGAA
    CACCAAGCAG ACTTCCAGAT CTATTCTGAG TACTGCAACA ACCACCCCAA TGCCTGCGTG
    GAGCTCTCCC GGCTCACGAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG
    CTGCAAAGGA TGATTGACAT CTCGCTGGAT GGCTTTCTGC TGACCCCGGT GCAGAAGATC
    TGCAAGTATC CCCTCCAGCT GGCGGAGCTG CTCAAGTACA CACACCCCCA GCACAGGGAC
    TTTAAGAATG TTGAAGCTGC CTTGCACGCC ATGAAGAATG TGGCCCAGCT CATCAACGAA
    CGGAAACGAA GACTTGAAAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC
    TGGGAGGGGG AAGACCTTCT GGTCAGGAGC TCGGAACTCA TCCACTCGGG GGAGCTGACC
    CGCGTGACAC AGCCTCAAGC CAGGAGTCAG CAGAGAATGT TTTTTCTCTT TGACCGCCAG
    CTCATCTACT GTAAAAAGGA TCTGCTTCGC CGGGACGTGC TGTACTACAA GGGCCGGCTG
    GACATGGATG ACCTGGAGGT GGTAGATGTG GAAGATGGGA AGGACCGAGA CCTCCATGTG
    AGTGTCAAGA ACGCCTTCCG TCTGTACTGT GGTACCACGG GGGACAGCCA CCTACTGTGT
    GCCAGGAAGC CAGAACAGAA GCAGCGCTGG CTGAAGGCCT TTGCCAGAGA GAGGGAGCAG
    GTGCGGCTGG ACCAGGAGAC AGGCTTCTCC ATCACCGAGC TGCAGAGGAA GCAGGCCATG
    CTGAACGCCA GCAAGCAGCA GGCCACGGGG AAGCCTAAAG CTGTGGGAAG GCCAGGATAC
    CTGACCCGCC ACAAGCATCC ATCATTGCCT GCCAGCCGGC CCCAGCAGCA AGTGTTGGTG
    CTAGCAGAGC CCAGGCGGAA GCCATCTAAC TTCTGGCACA GCATCAGCAG ACTGGCGCCT
    TTCCGCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109469.1
    CDS151..2082
    Translation

    Target ORF information:

    RefSeq Version XM_030253609.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253609.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGGGGG ACCCGAAGCT ACGGCAGTGC GGCGGGGATG CTGCTGGCGA CCCCGCCGGC 
    CTGTGGCTGG AGCTCGGAGC CGCTTGCCCC GGGGCGCCGC GCTGCCCCAG CGAGTCCAGT
    GGCCCTACCA GCGGCGATCT GGGCACAAGC AGCAGCAGCA GCACTGGGGT GTCCCCGGGT
    TCCGACTCGG ACAGCAGCGG CGTGGTGTGC AGTGGCCGCG CTGCGGGCAT GCGCGGCGCC
    CCGTCGCGCT CCCGGAGCCT GGAGAGCCTG CGCTCGGCCT CGGCGGGGAT GCCTGACGGA
    ACTCTGGACA CGGTGTGCGC CGAGGAGACA GGCAGTGAGG AGGACCTGTA TGAAGAGCTT
    CACAACTCTG GCCACCACTA CAGCCACCCT AGAGGAGGTG GAGAGCAACT GGCCATCAAT
    GAGCTCATCA GTGATGGCAG TGTGGTGTGT GCTGAAGCCC TGTGGGACCA TGTCACCATG
    GATGACCAGG AGCTGGGCTT CAAAGCTGGG GATGTCATCG AAGTGATGGA TGCCACCAAC
    AGGGAATGGT GGTGGGGCCG AGTGGCAGAT GGCGAGGGGT GGTTCCCTGC CAGCTTTGTA
    CGGCTGCGCG TGAACCAGGA TGAGCCCGCG GATGACTATG AGGCCCCGCG GGCCGGGGCT
    AGAGAGGCCG ATGACAGCGG TCCAGAGGCT CAGAGCTGCA AGGACCAGAT GCGCACAAAT
    GTCATCAATG AGATCCTCAG CACTGAGCGA GACTACATCA AGCACCTTCG CGACATCTGC
    GAGGGCTATG TCCGCCAGTG CCGCAAACGG GAGGATATGT TCAGTGAAGA GCAGCTGCGC
    ACCATCTTTG GGAACATCGA GGACATCTAC CGCTGCCAAA AGGCATTTGT GAAGGCTTTG
    GAGCAGAAGT TCAACACGGA GCGGCCACAC CTGAGCGAGT TGGGGGCCTG CTTTCTGGAA
    CACCAAGCAG ACTTCCAGAT CTATTCTGAG TACTGCAACA ACCACCCCAA TGCCTGCGTG
    GAGCTCTCCC GGCTCACGAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG
    CTGCAAAGGA TGATTGACAT CTCGCTGGAT GGCTTTCTGC TGACCCCGGT GCAGAAGATC
    TGCAAGTATC CCCTCCAGCT GGCGGAGCTG CTCAAGTACA CACACCCCCA GCACAGGGAC
    TTTAAGAATG TTGAAGCTGC CTTGCACGCC ATGAAGAATG TGGCCCAGCT CATCAACGAA
    CGGAAACGAA GACTTGAAAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC
    TGGGAGGGGG AAGACCTTCT GGTCAGGAGC TCGGAACTCA TCCACTCGGG GGAGCTGACC
    CGCGTGACAC AGCCTCAAGC CAGGAGTCAG CAGAGAATGT TTTTTCTCTT TGACCGCCAG
    CTCATCTACT GTAAAAAGGA TCTGCTTCGC CGGGACGTGC TGTACTACAA GGGCCGGCTG
    GACATGGATG ACCTGGAGGT GGTAGATGTG GAAGATGGGA AGGACCGAGA CCTCCATGTG
    AGTGTCAAGA ACGCCTTCCG TCTGTACTGT GGTACCACGG GGGACAGCCA CCTACTGTGT
    GCCAGGAAGC CAGAACAGAA GCAGCGCTGG CTGAAGGCCT TTGCCAGAGA GAGGGAGCAG
    GTGCGGCTGG ACCAGGAGAC AGGCTTCTCC ATCACCGAGC TGCAGAGGAA GCAGGCCATG
    CTGAACGCCA GCAAGCAGCA GGCCACGGGG AAGCCTAAAG CTGTGGGAAG GCCAGGATAC
    CTGACCCGCC ACAAGCATCC ATCATTGCCT GCCAGCCGGC CCCAGCAGCA AGTGTTGGTG
    CTAGCAGAGC CCAGGCGGAA GCCATCTAAC TTCTGGCACA GCATCAGCAG ACTGGCGCCT
    TTCCGCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85140
    Clone ID Related Accession (Same CDS sequence) XM_030253611.1
    Accession Version XM_030253611.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAAGGAC AAGAGCATGC TCTTCTGCCT TGCCCAGTGT CGTTCTCATC TGAAATGTCT 
    GTAGCAGTCT TGAGCAGAAT TACAAGGACA CAGAGTGCTG TTGCTAGGAG GAAGTGGGAG
    GAGAGAAAAG GAAGAGAGGG TCAGCTTCTT TCTATCAAGA CTTCCCCTCA CACCTCTCAA
    GCTCCAGGGA TGCCTGACGG AACTCTGGAC ACGGTGTGCG CCGAGGAGAC AGGCAGTGAG
    GAGGACCTGT ATGAAGAGCT TCACAACTCT GGCCACCACT ACAGCCACCC TAGAGGAGGT
    GGAGAGCAAC TGGCCATCAA TGAGCTCATC AGTGATGGCA GTGTGGTGTG TGCTGAAGCC
    CTGTGGGACC ATGTCACCAT GGATGACCAG GAGCTGGGCT TCAAAGCTGG GGATGTCATC
    GAAGTGATGG ATGCCACCAA CAGGGAATGG TGGTGGGGCC GAGTGGCAGA TGGCGAGGGG
    TGGTTCCCTG CCAGCTTTGT ACGGCTGCGC GTGAACCAGG ATGAGCCCGC GGATGACTAT
    GAGGCCCCGC GGGCCGGGGC TAGAGAGGCC GATGACAGCG GTCCAGAGGC TCAGAGCTGC
    AAGGACCAGA TGCGCACAAA TGTCATCAAT GAGATCCTCA GCACTGAGCG AGACTACATC
    AAGCACCTTC GCGACATCTG CGAGGGCTAT GTCCGCCAGT GCCGCAAACG GGAGGATATG
    TTCAGTGAAG AGCAGCTGCG CACCATCTTT GGGAACATCG AGGACATCTA CCGCTGCCAA
    AAGGCATTTG TGAAGGCTTT GGAGCAGAAG TTCAACACGG AGCGGCCACA CCTGAGCGAG
    TTGGGGGCCT GCTTTCTGGA ACACCAAGCA GACTTCCAGA TCTATTCTGA GTACTGCAAC
    AACCACCCCA ATGCCTGCGT GGAGCTCTCC CGGCTCACGA AGCTCAGCAA GTACGTGTAC
    TTCTTCGAGG CCTGCCGGCT GCTGCAAAGG ATGATTGACA TCTCGCTGGA TGGCTTTCTG
    CTGACCCCGG TGCAGAAGAT CTGCAAGTAT CCCCTCCAGC TGGCGGAGCT GCTCAAGTAC
    ACACACCCCC AGCACAGGGA CTTTAAGAAT GTTGAAGCTG CCTTGCACGC CATGAAGAAT
    GTGGCCCAGC TCATCAACGA ACGGAAACGA AGACTTGAAA ACATCGACAA GATTGCTCAG
    TGGCAGAGCT CCATAGAGGA CTGGGAGGGG GAAGACCTTC TGGTCAGGAG CTCGGAACTC
    ATCCACTCGG GGGAGCTGAC CCGCGTGACA CAGCCTCAAG CCAGGAGTCA GCAGAGAATG
    TTTTTTCTCT TTGACCGCCA GCTCATCTAC TGTAAAAAGG ATCTGCTTCG CCGGGACGTG
    CTGTACTACA AGGGCCGGCT GGACATGGAT GACCTGGAGG TGGTAGATGT GGAAGATGGG
    AAGGACCGAG ACCTCCATGT GAGTGTCAAG AACGCCTTCC GTCTGTACTG TGGTACCACG
    GGGGACAGCC ACCTACTGTG TGCCAGGAAG CCAGAACAGA AGCAGCGCTG GCTGAAGGCC
    TTTGCCAGAG AGAGGGAGCA GGTGCGGCTG GACCAGGAGA CAGGCTTCTC CATCACCGAG
    CTGCAGAGGA AGCAGGCCAT GCTGAACGCC AGCAAGCAGC AGGCCACGGG GAAGCCTAAA
    GCTGTGGGAA GGCCAGGATA CCTGACCCGC CACAAGCATC CATCATTGCC TGCCAGCCGG
    CCCCAGCAGC AAGTGTTGGT GCTAGCAGAG CCCAGGCGGA AGCCATCTAA CTTCTGGCAC
    AGCATCAGCA GACTGGCGCC TTTCCGCAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109471.1
    CDS111..1943
    Translation

    Target ORF information:

    RefSeq Version XM_030253611.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253611.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAAGGAC AAGAGCATGC TCTTCTGCCT TGCCCAGTGT CGTTCTCATC TGAAATGTCT 
    GTAGCAGTCT TGAGCAGAAT TACAAGGACA CAGAGTGCTG TTGCTAGGAG GAAGTGGGAG
    GAGAGAAAAG GAAGAGAGGG TCAGCTTCTT TCTATCAAGA CTTCCCCTCA CACCTCTCAA
    GCTCCAGGGA TGCCTGACGG AACTCTGGAC ACGGTGTGCG CCGAGGAGAC AGGCAGTGAG
    GAGGACCTGT ATGAAGAGCT TCACAACTCT GGCCACCACT ACAGCCACCC TAGAGGAGGT
    GGAGAGCAAC TGGCCATCAA TGAGCTCATC AGTGATGGCA GTGTGGTGTG TGCTGAAGCC
    CTGTGGGACC ATGTCACCAT GGATGACCAG GAGCTGGGCT TCAAAGCTGG GGATGTCATC
    GAAGTGATGG ATGCCACCAA CAGGGAATGG TGGTGGGGCC GAGTGGCAGA TGGCGAGGGG
    TGGTTCCCTG CCAGCTTTGT ACGGCTGCGC GTGAACCAGG ATGAGCCCGC GGATGACTAT
    GAGGCCCCGC GGGCCGGGGC TAGAGAGGCC GATGACAGCG GTCCAGAGGC TCAGAGCTGC
    AAGGACCAGA TGCGCACAAA TGTCATCAAT GAGATCCTCA GCACTGAGCG AGACTACATC
    AAGCACCTTC GCGACATCTG CGAGGGCTAT GTCCGCCAGT GCCGCAAACG GGAGGATATG
    TTCAGTGAAG AGCAGCTGCG CACCATCTTT GGGAACATCG AGGACATCTA CCGCTGCCAA
    AAGGCATTTG TGAAGGCTTT GGAGCAGAAG TTCAACACGG AGCGGCCACA CCTGAGCGAG
    TTGGGGGCCT GCTTTCTGGA ACACCAAGCA GACTTCCAGA TCTATTCTGA GTACTGCAAC
    AACCACCCCA ATGCCTGCGT GGAGCTCTCC CGGCTCACGA AGCTCAGCAA GTACGTGTAC
    TTCTTCGAGG CCTGCCGGCT GCTGCAAAGG ATGATTGACA TCTCGCTGGA TGGCTTTCTG
    CTGACCCCGG TGCAGAAGAT CTGCAAGTAT CCCCTCCAGC TGGCGGAGCT GCTCAAGTAC
    ACACACCCCC AGCACAGGGA CTTTAAGAAT GTTGAAGCTG CCTTGCACGC CATGAAGAAT
    GTGGCCCAGC TCATCAACGA ACGGAAACGA AGACTTGAAA ACATCGACAA GATTGCTCAG
    TGGCAGAGCT CCATAGAGGA CTGGGAGGGG GAAGACCTTC TGGTCAGGAG CTCGGAACTC
    ATCCACTCGG GGGAGCTGAC CCGCGTGACA CAGCCTCAAG CCAGGAGTCA GCAGAGAATG
    TTTTTTCTCT TTGACCGCCA GCTCATCTAC TGTAAAAAGG ATCTGCTTCG CCGGGACGTG
    CTGTACTACA AGGGCCGGCT GGACATGGAT GACCTGGAGG TGGTAGATGT GGAAGATGGG
    AAGGACCGAG ACCTCCATGT GAGTGTCAAG AACGCCTTCC GTCTGTACTG TGGTACCACG
    GGGGACAGCC ACCTACTGTG TGCCAGGAAG CCAGAACAGA AGCAGCGCTG GCTGAAGGCC
    TTTGCCAGAG AGAGGGAGCA GGTGCGGCTG GACCAGGAGA CAGGCTTCTC CATCACCGAG
    CTGCAGAGGA AGCAGGCCAT GCTGAACGCC AGCAAGCAGC AGGCCACGGG GAAGCCTAAA
    GCTGTGGGAA GGCCAGGATA CCTGACCCGC CACAAGCATC CATCATTGCC TGCCAGCCGG
    CCCCAGCAGC AAGTGTTGGT GCTAGCAGAG CCCAGGCGGA AGCCATCTAA CTTCTGGCAC
    AGCATCAGCA GACTGGCGCC TTTCCGCAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85141
    Clone ID Related Accession (Same CDS sequence) XM_030253613.1
    Accession Version XM_030253613.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCGGGGG ACCCGAAGCT ACGGCAGTGC GGCGGGGATG CTGCTGGCGA CCCCGCCGGC 
    CTGTGGCTGG AGCTCGGAGC CGCTTGCCCC GGGGCGCCGC GCTGCCCCAG CGAGTCCAGT
    GGCCCTACCA GCGGCGATCT GGGCACAAGC AGCAGCAGCA GCACTGGGGT GTCCCCGGGT
    TCCGACTCGG ACAGCAGCGG CGTGGTGTGC AGTGGCCGCG CTGCGGGCAT GCGCGGCGCC
    CCGTCGCGCT CCCGGAGCCT GGAGAGCCTG CGCTCGGCCT CGGCGGGGAT GCCTGACGGA
    ACTCTGGACA CGGTGTGCGC CGAGGAGACA GGCAGTGAGG AGGACCTGTA TGAAGAGCTT
    CACAACTCTG GCCACCACTA CAGCCACCCT AGAGGAGGTG GAGAGCAACT GGCCATCAAT
    GAGCTCATCA GTGATGGCAG TGTGGTGTGT GCTGAAGCCC TGTGGGACCA TGTCACCATG
    GATGACCAGG AGCTGGGCTT CAAAGCTGGG GATGTCATCG AAGTGATGGA TGCCACCAAC
    AGGGAATGGT GGTGGGGCCG AGTGGCAGAT GGCGAGGGGT GGTTCCCTGC CAGCTTTGTA
    CGGCTGCGCG TGAACCAGGA TGAGCCCGCG GATGACTATG AGGCCCCGCG GGCCGGGGCT
    AGAGAGGCCG ATGACAGCGG TCCAGAGGCT CAGAGCTGCA AGGACCAGAT GCGCACAAAT
    GTCATCAATG AGATCCTCAG CACTGAGCGA GACTACATCA AGCACCTTCG CGACATCTGC
    GAGGGCTATG TCCGCCAGTG CCGCAAACGG GAGGATATGT TCAGTGAAGA GCAGCTGCGC
    ACCATCTTTG GGAACATCGA GGACATCTAC CGCTGCCAAA AGGCATTTGT GAAGGCTTTG
    GAGCAGAAGT TCAACACGGA GCGGCCACAC CTGAGCGAGT TGGGGGCCTG CTTTCTGGAA
    CACCAAGCAG ACTTCCAGAT CTATTCTGAG TACTGCAACA ACCACCCCAA TGCCTGCGTG
    GAGCTCTCCC GGCTCACGAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG
    CTGCAAAGGA TGATTGACAT CTCGCTGGAT GGCTTTCTGC TGACCCCGGT GCAGAAGATC
    TGCAAGTATC CCCTCCAGCT GGCGGAGCTG CTCAAGTACA CACACCCCCA GCACAGGGAC
    TTTAAGAATG TTGAAGCTGC CTTGCACGCC ATGAAGAATG TGGCCCAGCT CATCAACGAA
    CGGAAACGAA GACTTGAAAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC
    TGGGAGGGGG AAGACCTTCT GGTCAGGAGC TCGGAACTCA TCCACTCGGG GGAGCTGACC
    CGCGTGACAC AGCCTCAAGC CAGGAGTCAG CAGAGAATGT TTTTTCTCTT TGACCGCCAG
    CTCATCTACT GTAAAAAGGA TCTGCTTCGC CGGGACGTGC TGTACTACAA GGGCCGGCTG
    GACATGGATG ACCTGGAGGT GGTAGATGTG GAAGATGGGA AGGACCGAGA CCTCCATGTG
    AGTGTCAAGA ACGCCTTCCG TCTGTACTGT GGTACCACGG GGGACAGCCA CCTACTGTGT
    GCCAGGAAGC CAGAACAGAA GCAGCGCTGG CTGAAGGCCT TTGCCAGAGA GAGGGAGCAG
    GCTTCTCCAT CACCGAGCTG CAGAGGAAGC AGGCCATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109473.1
    CDS151..1872
    Translation

    Target ORF information:

    RefSeq Version XM_030253613.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253613.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCGGGGG ACCCGAAGCT ACGGCAGTGC GGCGGGGATG CTGCTGGCGA CCCCGCCGGC 
    CTGTGGCTGG AGCTCGGAGC CGCTTGCCCC GGGGCGCCGC GCTGCCCCAG CGAGTCCAGT
    GGCCCTACCA GCGGCGATCT GGGCACAAGC AGCAGCAGCA GCACTGGGGT GTCCCCGGGT
    TCCGACTCGG ACAGCAGCGG CGTGGTGTGC AGTGGCCGCG CTGCGGGCAT GCGCGGCGCC
    CCGTCGCGCT CCCGGAGCCT GGAGAGCCTG CGCTCGGCCT CGGCGGGGAT GCCTGACGGA
    ACTCTGGACA CGGTGTGCGC CGAGGAGACA GGCAGTGAGG AGGACCTGTA TGAAGAGCTT
    CACAACTCTG GCCACCACTA CAGCCACCCT AGAGGAGGTG GAGAGCAACT GGCCATCAAT
    GAGCTCATCA GTGATGGCAG TGTGGTGTGT GCTGAAGCCC TGTGGGACCA TGTCACCATG
    GATGACCAGG AGCTGGGCTT CAAAGCTGGG GATGTCATCG AAGTGATGGA TGCCACCAAC
    AGGGAATGGT GGTGGGGCCG AGTGGCAGAT GGCGAGGGGT GGTTCCCTGC CAGCTTTGTA
    CGGCTGCGCG TGAACCAGGA TGAGCCCGCG GATGACTATG AGGCCCCGCG GGCCGGGGCT
    AGAGAGGCCG ATGACAGCGG TCCAGAGGCT CAGAGCTGCA AGGACCAGAT GCGCACAAAT
    GTCATCAATG AGATCCTCAG CACTGAGCGA GACTACATCA AGCACCTTCG CGACATCTGC
    GAGGGCTATG TCCGCCAGTG CCGCAAACGG GAGGATATGT TCAGTGAAGA GCAGCTGCGC
    ACCATCTTTG GGAACATCGA GGACATCTAC CGCTGCCAAA AGGCATTTGT GAAGGCTTTG
    GAGCAGAAGT TCAACACGGA GCGGCCACAC CTGAGCGAGT TGGGGGCCTG CTTTCTGGAA
    CACCAAGCAG ACTTCCAGAT CTATTCTGAG TACTGCAACA ACCACCCCAA TGCCTGCGTG
    GAGCTCTCCC GGCTCACGAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG
    CTGCAAAGGA TGATTGACAT CTCGCTGGAT GGCTTTCTGC TGACCCCGGT GCAGAAGATC
    TGCAAGTATC CCCTCCAGCT GGCGGAGCTG CTCAAGTACA CACACCCCCA GCACAGGGAC
    TTTAAGAATG TTGAAGCTGC CTTGCACGCC ATGAAGAATG TGGCCCAGCT CATCAACGAA
    CGGAAACGAA GACTTGAAAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC
    TGGGAGGGGG AAGACCTTCT GGTCAGGAGC TCGGAACTCA TCCACTCGGG GGAGCTGACC
    CGCGTGACAC AGCCTCAAGC CAGGAGTCAG CAGAGAATGT TTTTTCTCTT TGACCGCCAG
    CTCATCTACT GTAAAAAGGA TCTGCTTCGC CGGGACGTGC TGTACTACAA GGGCCGGCTG
    GACATGGATG ACCTGGAGGT GGTAGATGTG GAAGATGGGA AGGACCGAGA CCTCCATGTG
    AGTGTCAAGA ACGCCTTCCG TCTGTACTGT GGTACCACGG GGGACAGCCA CCTACTGTGT
    GCCAGGAAGC CAGAACAGAA GCAGCGCTGG CTGAAGGCCT TTGCCAGAGA GAGGGAGCAG
    GCTTCTCCAT CACCGAGCTG CAGAGGAAGC AGGCCATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu84554
    Clone ID Related Accession (Same CDS sequence) NM_001368773.1
    Accession Version NM_001368773.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 4 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC157813.6, AC107766.14 and AC107738.16. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCTCGGTG TGGTGCACTT CCTTCGCAGC TTCTTTAAGA CTCCAGAGCC AGGTGTGCCC 
    CCTCCAGGTG AGGAAGAGTG TGAAGACAAC CAGCTCCACC CAGGCCCTGC AGACTCGGAG
    GAACAGGAAA CAAACCCCGA GGACTTGGAG ACACTGTCCC ATAAGAGCGA AAGTCGATCC
    GGATCCGATA CAAAAACAGA TCCCTTTGAA AGTGCTTCTG AGGCGGAGTC CCTCCCGGGT
    GACCTCACTG GTGGAGTTGG CCTCTCCGAA AGAGAAGCAG GCTGGGGATG CCCTGAGGAC
    TCAACAGTTA GACTTCCCCA AGGGCGGCAT TTGGTCTGTG ACGAAGGGCT CCATACAGAG
    GAAGAGCCGG ATCTACCCTC AGGCTTAAAG GCAGGTTCTC AGCTAAGTAA ATTGCACATG
    TTGACTGCTG GAAGACGGGA TGAAGCATGC CCCTGCAGCC GTGTGGCTAG CTTTGTACAG
    GAGTCTGTGC CAGCATCTGT GTGCTTCTCG AAGCATGCCA GCAGCAACAG CAGTCCCCAC
    CTGCAACAGG CCAGGTCTAG GGGCTATCTG GATGCCCCTG TGGTCTGTCC TTGCTTTCGT
    CGACACTGTG AACTAGGTCA GAGCTGGCCA CAAATCCACA TCAGGACTGC AGCAGCAAAG
    ATACCTCCCA CAGGCTCACA GCACAATGAC TTCCCTCTGT GGATCAGGAC AAAGAAGGGG
    CCTGCTCCGA GGTCCACAGT GGGCACAGAC TTGTTCTGGG TTCATCCCTT CCTTGACATA
    GCCTGTCAAC CTCGATTGGG CACACCTTAT CTCTTAGACA CCAAGTGTCA CCAGAGTACC
    CCAGAAAATC TTGGTGCCAA TGCCAAGCCA CCTCAAGGGT ACCTCTGCTG GCAGGTAAAG
    ACTCTGGTCA GGGCCCATTC TATAGCTTCA TTTCCTTTAG GTTGTCCTAA AGAGCCTCTG
    AAGCAGAAAG GTACAAATTG GCCCTACTTG CGGGAAGGGA CAGAAATAGA ACCCGACCAC
    CCAATCCTTC ACAAAACCCA GTCTCCTGGG CCCACCCAGC TGTCTCGTTT ACTTCCTACT
    TCTCAAAGCA GGGCCCCTCC TTTGCAGGGT GAGTGCAAGC TGAGTGATTG TAGCTTTAAG
    AGGGCATCTC CAGACACACC CTGTGTAGGC CTTCCACTGG AAAACCAGGT AGAACAGGCT
    TCTAGGTCAT GCCCAGCCCC TTCCCACCTT ACTTCAGAAT TGCTGAAGCT CAACAGCCAA
    GAGGAAGTGC CCAGGCCGGC TGAATGCAAG CCAGGGCACA GCCCAGAAAG CAGACTTGAG
    GAGCCCAAAG GTGTCCACCC TGATGATGTT ATCAGTAAAC AACAGTGCCT GCTAAACATC
    TCCGGAGAGG CAGGAAGCCA GACGGAGAAC TACCCATCAA TGAGTGCTCT CCAGGAGGAG
    AGAAAGCCGC CTGATAGGGC CTCCCTCTTC CCACAACACT GCTGCCATGG TGAGGTACCT
    GTGTGCAACA GAATGCTTGC AGGTTCCTGG GAAGAAGAGA GCGATAGCGC CTGTGCCCCA
    AGGACAGCCC TGAAATCAAG TGTCATTGGC TCTTTAGAAG CAGCAGAAGA GGGAGTGGTT
    CTAGACCTCC AACGCAATGG GAAGGCCTTG CAAAGTCTTT TGGACTTCCC AACATTGGAA
    GTATCTCACC GCAACCCTGG GAGTGAGTGT GCACAGGGAC CTGGGGATGA CCCGGAGCCT
    TCCCCTTCTG GCCAGCGGCA GGCTCCAGGA GCCCACGGGG CCTCCAGGCA CAGCTGCAAA
    TTCATTATGA TAGCAGTAAA GCAGGAAGGA AGGCAGACTT CTACAAGGAA GGCTCACTGT
    CTCATAGAAC CACAGTGTGG GCACCTTCCA GAGAAGGGGA CAAGCGCCAC GGCTTATGAG
    TCTTCCAGGG CCTCAGAAAT GCCCGTGTTT GGCAATGAAT GTGAGCTGCT GGGATGTCCA
    GGGGCAGAGG TGGATGGTGG GGCTCTTCAG CTGCTGGCCC AAGGCATAGA GCACGTGAGA
    GTGCCAGTCT CTAGGACTTC TTCGGATGAC ACTTTGAGCT CAGAGGAGAC ACTGGAGGGC
    GAGCTGCTTG GGGAAAGATT GGACTTAGGG TCTGAGTGTG TACACACCGA GCAGAACCAG
    GAGTTGCCCA GCTCAGATCG CAAACGAGGA AGGTGTGGCA CAGGCGATGC GGAGAATGCG
    GAAATCTGCG CCTGCGCGGG CGAGGTAGAG GGTCCCCTTT CAAGAGTCTT GGCTTCAAGC
    ACCACAACAC TGGAGTTCCA GGAACCATGC GGTGGGTTTC AGCAGAGTGT GCTGGAGTCA
    GAGCAGCACC CTGCCTGTCT TCAGATGACC CCTTTAAGAA CTGACATGCC CTGGTCCAGG
    GAGATGCCTG GCGGGGACAT ACCCTACAGC CAGGCCTTAA CCTCAGAGGA CTTGAACCCA
    GTTTGCCCTA GCCCCGAAGA AAGGGTCCCC CACAGTCCCT GCCAGGGGGA ACCACCTCTG
    CACCACGTGC ACCAGAGCGT ATCTCTGGAG ACCAAATCTG GTGTGATTCA AGTAGGGACA
    TCTGGGGACC TCAGCGGTGA AGGACCTTCC CCAGAAAGCT ACAACATCAA GAGACTGAAA
    ACAACAGAAA GCAAGATCAA AGCCAGGCTG GCCTTAGCCC ATAAGACCTT TACCAATTTT
    TTTGAAGCCA AAGTTATGGA GAAAACAAAT ACTGGCTCTG TCAAAGGCGA GAAAGAGAAG
    CGCAGGCGAG CCCGGGGCTC CTGGCGTGCG TTTCTGAGAA GCAAAGATGC GGAAGGCTCC
    AAACGACCTG CTTTAGTGAG TGGGGTTCCA GAACCAGGCT TTCCCCAACT CCTCTGCTCT
    TCCCCACCAG CCACCAGAGG CCACTGTGAG AAGTGGACGA TTGACACCGA CAGCCATGTA
    TTTGGGGAGC ACTGGACACC CCTAAACTCC CCTGCAACTC TGTCCTCCAG CCATCTGGCT
    TCTCCGGAAC ACCGGAGGAG AAGTGAACCG ACCATAAGAC GCACTGCTGC CCAGGAAAGC
    AGTACGCACC TCTCTTCTGG AGTCTTCCCA AAGTCTTCCT GGCTGACCTC TGCCACCAGC
    CCCGGAGCAG CGCAGATTGA CATCAGTTTT GCCCTCCCCT GCAGCTCTGC CTGCCATTTG
    ACCTATGAGA ACCAAGGCAT GCCCTGCAGA CCCACAAGCC CCAAACCCAG GAGTCCCATG
    GCTGGGGCGC AGAGGATGGA TCTCTGCCTT AGAGGCAGGA CTAGTGCTGT CTCCATGGTC
    TCTCTCAGAA GTGACAAGGA CGCCAACAGA AGTTCAGAAG TCCCCGAACG GCCCAAGGCA
    TCCAAGGCCA GGGCGAGCCT CTTACTCTCT CTGCAAACAC TAAACCAGAA CGACCAGAAG
    GAAGACAAGA GCCAGTGCCA GTGTTACCAT GGCCTGGGCA CAGTGCCCTT CCTTAGGGAC
    CATCCTGGGA ATGAGAGCTA CATCGCCCAG GAAAGCCCGC CAGACAAGAA GCGCAATTGT
    TCCCATGATC AGAAAACTTA CACAGAGCCT CCTCACAGGC CGGTATCTGC CACTGAAGTG
    ACACGGACCC TCCCCTCCAT GTCCGCAGAG CAAGTCCCCA GAGAGGTCCC TTTACAGTTC
    AGGAGGGCCC CTCAACACTC ACACTTCAGC TTCGATGACA TATGGCTCGA AAAGGCTCAG
    AGGAGGAAGC TGAAGAAACA GATGCTGCTT AGACGACAGA TGTGCGCAGA GTTAGTGCCT
    GAGGACCCAG TTCAGTGCTG GAGGAAGACA ACCATCACCT CTCCAGAGTC TCCGCATCTT
    CCTAGAAGAA GCCATCTGCT CTCCCAGAGT GCCCCAATGG GACTGGACCA CACAGGCTGG
    CCAGAGCCCA CGTCTGACAC TGGGATGCCT GACGGAACTC TGGACACGGT GTGCGCCGAG
    GAGACAGGCA GTGAGGAGGA CCTGTATGAA GAGCTTCACA ACTCTGGCCA CCACTACAGC
    CACCCTAGAG GAGGTGGAGA GCAACTGGCC ATCAATGAGC TCATCAGTGA TGGCAGTGTG
    GTGTGTGCTG AAGCCCTGTG GGACCATGTC ACCATGGATG ACCAGGAGCT GGGCTTCAAA
    GCTGGGGATG TCATCGAAGT GATGGATGCC ACCAACAGGG AATGGTGGTG GGGCCGAGTG
    GCAGATGGCG AGGGGTGGTT CCCTGCCAGC TTTGTACGGC TGCGCGTGAA CCAGGATGAG
    CCCGCGGATG ACTATGAGGC CCCGCGGGCC GGGGCTAGAG AGGCCGATGA CAGCGGTCCA
    GAGGCTCAGA GCTGCAAGGA CCAGATGCGC ACAAATGTCA TCAATGAGAT CCTCAGCACT
    GAGCGAGACT ACATCAAGCA CCTTCGCGAC ATCTGCGAGG GCTATGTCCG CCAGTGCCGC
    AAACGGGAGG ATATGTTCAG TGAAGAGCAG CTGCGCACCA TCTTTGGGAA CATCGAGGAC
    ATCTACCGCT GCCAAAAGGC ATTTGTGAAG GCTTTGGAGC AGAAGTTCAA CACGGAGCGG
    CCACACCTGA GCGAGTTGGG GGCCTGCTTT CTGGAACACC AAGCAGACTT CCAGATCTAT
    TCTGAGTACT GCAACAACCA CCCCAATGCC TGCGTGGAGC TCTCCCGGCT CACGAAGCTC
    AGCAAGTACG TGTACTTCTT CGAGGCCTGC CGGCTGCTGC AAAGGATGAT TGACATCTCG
    CTGGATGGCT TTCTGCTGAC CCCGGTGCAG AAGATCTGCA AGTATCCCCT CCAGCTGGCG
    GAGCTGCTCA AGTACACACA CCCCCAGCAC AGGGACTTTA AGAATGTTGA AGCTGCCTTG
    CACGCCATGA AGAATGTGGC CCAGCTCATC AACGAACGGA AACGAAGACT TGAAAACATC
    GACAAGATTG CTCAGTGGCA GAGCTCCATA GAGGACTGGG AGGGGGAAGA CCTTCTGGTC
    AGGAGCTCGG AACTCATCCA CTCGGGGGAG CTGACCCGCG TGACACAGCC TCAAGCCAGG
    AGTCAGCAGA GAATGTTTTT TCTCTTTGAC CGCCAGCTCA TCTACTGTAA AAAGGATCTG
    CTTCGCCGGG ACGTGCTGTA CTACAAGGGC CGGCTGGACA TGGATGACCT GGAGGTGGTA
    GATGTGGAAG ATGGGAAGGA CCGAGACCTC CATGTGAGTG TCAAGAACGC CTTCCGTCTG
    TACTGTGGTA CCACGGGGGA CAGCCACCTA CTGTGTGCCA GGAAGCCAGA ACAGAAGCAG
    CGCTGGCTGA AGGCCTTTGC CAGAGAGAGG GAGCAGGTGC GGCTGGACCA GGAGACAGGC
    TTCTCCATCA CCGAGCTGCA GAGGAAGCAG GCCATGCTGA ACGCCAGCAA GCAGCAGGCC
    ACGGGGAAGC CTAAAGCTGT GGGAAGGCCA GGATACCTGA CCCGCCACAA GCATCCATCA
    TTGCCTGCCA GCCGGCCCCA GCAGCAAGTG TTGGTGCTAG CAGAGCCCAG GCGGAAGCCA
    TCTAACTTCT GGCACAGCAT CAGCAGACTG GCGCCTTTCC GCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001355702.1
    CDS43..5610
    Translation

    Target ORF information:

    RefSeq Version NM_001368773.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001368773.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCTCGGTG TGGTGCACTT CCTTCGCAGC TTCTTTAAGA CTCCAGAGCC AGGTGTGCCC 
    CCTCCAGGTG AGGAAGAGTG TGAAGACAAC CAGCTCCACC CAGGCCCTGC AGACTCGGAG
    GAACAGGAAA CAAACCCCGA GGACTTGGAG ACACTGTCCC ATAAGAGCGA AAGTCGATCC
    GGATCCGATA CAAAAACAGA TCCCTTTGAA AGTGCTTCTG AGGCGGAGTC CCTCCCGGGT
    GACCTCACTG GTGGAGTTGG CCTCTCCGAA AGAGAAGCAG GCTGGGGATG CCCTGAGGAC
    TCAACAGTTA GACTTCCCCA AGGGCGGCAT TTGGTCTGTG ACGAAGGGCT CCATACAGAG
    GAAGAGCCGG ATCTACCCTC AGGCTTAAAG GCAGGTTCTC AGCTAAGTAA ATTGCACATG
    TTGACTGCTG GAAGACGGGA TGAAGCATGC CCCTGCAGCC GTGTGGCTAG CTTTGTACAG
    GAGTCTGTGC CAGCATCTGT GTGCTTCTCG AAGCATGCCA GCAGCAACAG CAGTCCCCAC
    CTGCAACAGG CCAGGTCTAG GGGCTATCTG GATGCCCCTG TGGTCTGTCC TTGCTTTCGT
    CGACACTGTG AACTAGGTCA GAGCTGGCCA CAAATCCACA TCAGGACTGC AGCAGCAAAG
    ATACCTCCCA CAGGCTCACA GCACAATGAC TTCCCTCTGT GGATCAGGAC AAAGAAGGGG
    CCTGCTCCGA GGTCCACAGT GGGCACAGAC TTGTTCTGGG TTCATCCCTT CCTTGACATA
    GCCTGTCAAC CTCGATTGGG CACACCTTAT CTCTTAGACA CCAAGTGTCA CCAGAGTACC
    CCAGAAAATC TTGGTGCCAA TGCCAAGCCA CCTCAAGGGT ACCTCTGCTG GCAGGTAAAG
    ACTCTGGTCA GGGCCCATTC TATAGCTTCA TTTCCTTTAG GTTGTCCTAA AGAGCCTCTG
    AAGCAGAAAG GTACAAATTG GCCCTACTTG CGGGAAGGGA CAGAAATAGA ACCCGACCAC
    CCAATCCTTC ACAAAACCCA GTCTCCTGGG CCCACCCAGC TGTCTCGTTT ACTTCCTACT
    TCTCAAAGCA GGGCCCCTCC TTTGCAGGGT GAGTGCAAGC TGAGTGATTG TAGCTTTAAG
    AGGGCATCTC CAGACACACC CTGTGTAGGC CTTCCACTGG AAAACCAGGT AGAACAGGCT
    TCTAGGTCAT GCCCAGCCCC TTCCCACCTT ACTTCAGAAT TGCTGAAGCT CAACAGCCAA
    GAGGAAGTGC CCAGGCCGGC TGAATGCAAG CCAGGGCACA GCCCAGAAAG CAGACTTGAG
    GAGCCCAAAG GTGTCCACCC TGATGATGTT ATCAGTAAAC AACAGTGCCT GCTAAACATC
    TCCGGAGAGG CAGGAAGCCA GACGGAGAAC TACCCATCAA TGAGTGCTCT CCAGGAGGAG
    AGAAAGCCGC CTGATAGGGC CTCCCTCTTC CCACAACACT GCTGCCATGG TGAGGTACCT
    GTGTGCAACA GAATGCTTGC AGGTTCCTGG GAAGAAGAGA GCGATAGCGC CTGTGCCCCA
    AGGACAGCCC TGAAATCAAG TGTCATTGGC TCTTTAGAAG CAGCAGAAGA GGGAGTGGTT
    CTAGACCTCC AACGCAATGG GAAGGCCTTG CAAAGTCTTT TGGACTTCCC AACATTGGAA
    GTATCTCACC GCAACCCTGG GAGTGAGTGT GCACAGGGAC CTGGGGATGA CCCGGAGCCT
    TCCCCTTCTG GCCAGCGGCA GGCTCCAGGA GCCCACGGGG CCTCCAGGCA CAGCTGCAAA
    TTCATTATGA TAGCAGTAAA GCAGGAAGGA AGGCAGACTT CTACAAGGAA GGCTCACTGT
    CTCATAGAAC CACAGTGTGG GCACCTTCCA GAGAAGGGGA CAAGCGCCAC GGCTTATGAG
    TCTTCCAGGG CCTCAGAAAT GCCCGTGTTT GGCAATGAAT GTGAGCTGCT GGGATGTCCA
    GGGGCAGAGG TGGATGGTGG GGCTCTTCAG CTGCTGGCCC AAGGCATAGA GCACGTGAGA
    GTGCCAGTCT CTAGGACTTC TTCGGATGAC ACTTTGAGCT CAGAGGAGAC ACTGGAGGGC
    GAGCTGCTTG GGGAAAGATT GGACTTAGGG TCTGAGTGTG TACACACCGA GCAGAACCAG
    GAGTTGCCCA GCTCAGATCG CAAACGAGGA AGGTGTGGCA CAGGCGATGC GGAGAATGCG
    GAAATCTGCG CCTGCGCGGG CGAGGTAGAG GGTCCCCTTT CAAGAGTCTT GGCTTCAAGC
    ACCACAACAC TGGAGTTCCA GGAACCATGC GGTGGGTTTC AGCAGAGTGT GCTGGAGTCA
    GAGCAGCACC CTGCCTGTCT TCAGATGACC CCTTTAAGAA CTGACATGCC CTGGTCCAGG
    GAGATGCCTG GCGGGGACAT ACCCTACAGC CAGGCCTTAA CCTCAGAGGA CTTGAACCCA
    GTTTGCCCTA GCCCCGAAGA AAGGGTCCCC CACAGTCCCT GCCAGGGGGA ACCACCTCTG
    CACCACGTGC ACCAGAGCGT ATCTCTGGAG ACCAAATCTG GTGTGATTCA AGTAGGGACA
    TCTGGGGACC TCAGCGGTGA AGGACCTTCC CCAGAAAGCT ACAACATCAA GAGACTGAAA
    ACAACAGAAA GCAAGATCAA AGCCAGGCTG GCCTTAGCCC ATAAGACCTT TACCAATTTT
    TTTGAAGCCA AAGTTATGGA GAAAACAAAT ACTGGCTCTG TCAAAGGCGA GAAAGAGAAG
    CGCAGGCGAG CCCGGGGCTC CTGGCGTGCG TTTCTGAGAA GCAAAGATGC GGAAGGCTCC
    AAACGACCTG CTTTAGTGAG TGGGGTTCCA GAACCAGGCT TTCCCCAACT CCTCTGCTCT
    TCCCCACCAG CCACCAGAGG CCACTGTGAG AAGTGGACGA TTGACACCGA CAGCCATGTA
    TTTGGGGAGC ACTGGACACC CCTAAACTCC CCTGCAACTC TGTCCTCCAG CCATCTGGCT
    TCTCCGGAAC ACCGGAGGAG AAGTGAACCG ACCATAAGAC GCACTGCTGC CCAGGAAAGC
    AGTACGCACC TCTCTTCTGG AGTCTTCCCA AAGTCTTCCT GGCTGACCTC TGCCACCAGC
    CCCGGAGCAG CGCAGATTGA CATCAGTTTT GCCCTCCCCT GCAGCTCTGC CTGCCATTTG
    ACCTATGAGA ACCAAGGCAT GCCCTGCAGA CCCACAAGCC CCAAACCCAG GAGTCCCATG
    GCTGGGGCGC AGAGGATGGA TCTCTGCCTT AGAGGCAGGA CTAGTGCTGT CTCCATGGTC
    TCTCTCAGAA GTGACAAGGA CGCCAACAGA AGTTCAGAAG TCCCCGAACG GCCCAAGGCA
    TCCAAGGCCA GGGCGAGCCT CTTACTCTCT CTGCAAACAC TAAACCAGAA CGACCAGAAG
    GAAGACAAGA GCCAGTGCCA GTGTTACCAT GGCCTGGGCA CAGTGCCCTT CCTTAGGGAC
    CATCCTGGGA ATGAGAGCTA CATCGCCCAG GAAAGCCCGC CAGACAAGAA GCGCAATTGT
    TCCCATGATC AGAAAACTTA CACAGAGCCT CCTCACAGGC CGGTATCTGC CACTGAAGTG
    ACACGGACCC TCCCCTCCAT GTCCGCAGAG CAAGTCCCCA GAGAGGTCCC TTTACAGTTC
    AGGAGGGCCC CTCAACACTC ACACTTCAGC TTCGATGACA TATGGCTCGA AAAGGCTCAG
    AGGAGGAAGC TGAAGAAACA GATGCTGCTT AGACGACAGA TGTGCGCAGA GTTAGTGCCT
    GAGGACCCAG TTCAGTGCTG GAGGAAGACA ACCATCACCT CTCCAGAGTC TCCGCATCTT
    CCTAGAAGAA GCCATCTGCT CTCCCAGAGT GCCCCAATGG GACTGGACCA CACAGGCTGG
    CCAGAGCCCA CGTCTGACAC TGGGATGCCT GACGGAACTC TGGACACGGT GTGCGCCGAG
    GAGACAGGCA GTGAGGAGGA CCTGTATGAA GAGCTTCACA ACTCTGGCCA CCACTACAGC
    CACCCTAGAG GAGGTGGAGA GCAACTGGCC ATCAATGAGC TCATCAGTGA TGGCAGTGTG
    GTGTGTGCTG AAGCCCTGTG GGACCATGTC ACCATGGATG ACCAGGAGCT GGGCTTCAAA
    GCTGGGGATG TCATCGAAGT GATGGATGCC ACCAACAGGG AATGGTGGTG GGGCCGAGTG
    GCAGATGGCG AGGGGTGGTT CCCTGCCAGC TTTGTACGGC TGCGCGTGAA CCAGGATGAG
    CCCGCGGATG ACTATGAGGC CCCGCGGGCC GGGGCTAGAG AGGCCGATGA CAGCGGTCCA
    GAGGCTCAGA GCTGCAAGGA CCAGATGCGC ACAAATGTCA TCAATGAGAT CCTCAGCACT
    GAGCGAGACT ACATCAAGCA CCTTCGCGAC ATCTGCGAGG GCTATGTCCG CCAGTGCCGC
    AAACGGGAGG ATATGTTCAG TGAAGAGCAG CTGCGCACCA TCTTTGGGAA CATCGAGGAC
    ATCTACCGCT GCCAAAAGGC ATTTGTGAAG GCTTTGGAGC AGAAGTTCAA CACGGAGCGG
    CCACACCTGA GCGAGTTGGG GGCCTGCTTT CTGGAACACC AAGCAGACTT CCAGATCTAT
    TCTGAGTACT GCAACAACCA CCCCAATGCC TGCGTGGAGC TCTCCCGGCT CACGAAGCTC
    AGCAAGTACG TGTACTTCTT CGAGGCCTGC CGGCTGCTGC AAAGGATGAT TGACATCTCG
    CTGGATGGCT TTCTGCTGAC CCCGGTGCAG AAGATCTGCA AGTATCCCCT CCAGCTGGCG
    GAGCTGCTCA AGTACACACA CCCCCAGCAC AGGGACTTTA AGAATGTTGA AGCTGCCTTG
    CACGCCATGA AGAATGTGGC CCAGCTCATC AACGAACGGA AACGAAGACT TGAAAACATC
    GACAAGATTG CTCAGTGGCA GAGCTCCATA GAGGACTGGG AGGGGGAAGA CCTTCTGGTC
    AGGAGCTCGG AACTCATCCA CTCGGGGGAG CTGACCCGCG TGACACAGCC TCAAGCCAGG
    AGTCAGCAGA GAATGTTTTT TCTCTTTGAC CGCCAGCTCA TCTACTGTAA AAAGGATCTG
    CTTCGCCGGG ACGTGCTGTA CTACAAGGGC CGGCTGGACA TGGATGACCT GGAGGTGGTA
    GATGTGGAAG ATGGGAAGGA CCGAGACCTC CATGTGAGTG TCAAGAACGC CTTCCGTCTG
    TACTGTGGTA CCACGGGGGA CAGCCACCTA CTGTGTGCCA GGAAGCCAGA ACAGAAGCAG
    CGCTGGCTGA AGGCCTTTGC CAGAGAGAGG GAGCAGGTGC GGCTGGACCA GGAGACAGGC
    TTCTCCATCA CCGAGCTGCA GAGGAAGCAG GCCATGCTGA ACGCCAGCAA GCAGCAGGCC
    ACGGGGAAGC CTAAAGCTGT GGGAAGGCCA GGATACCTGA CCCGCCACAA GCATCCATCA
    TTGCCTGCCA GCCGGCCCCA GCAGCAAGTG TTGGTGCTAG CAGAGCCCAG GCGGAAGCCA
    TCTAACTTCT GGCACAGCAT CAGCAGACTG GCGCCTTTCC GCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85135
    Clone ID Related Accession (Same CDS sequence) XM_030253601.1
    Accession Version XM_030253601.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGATGTGC TGGATCTGGA GACTCCAGAG CCAGGTGTGC CCCCTCCAGG TGAGGAAGAG 
    TGTGAAGACA ACCAGCTCCA CCCAGGCCCT GCAGACTCGG AGGAACAGGA AACAAACCCC
    GAGGACTTGG AGACACTGTC CCATAAGAGC GAAAGTCGAT CCGGATCCGA TACAAAAACA
    GATCCCTTTG AAAGTGCTTC TGAGGCGGAG TCCCTCCCGG GTGACCTCAC TGGTGGAGTT
    GGCCTCTCCG AAAGAGAAGC AGGCTGGGGA TGCCCTGAGG ACTCAACAGT TAGACTTCCC
    CAAGGGCGGC ATTTGGTCTG TGACGAAGGG CTCCATACAG AGGAAGAGCC GGATCTACCC
    TCAGGCTTAA AGGCAGGTTC TCAGCTAAGT AAATTGCACA TGTTGACTGC TGGAAGACGG
    GATGAAGCAT GCCCCTGCAG CCGTGTGGCT AGCTTTGTAC AGGAGTCTGT GCCAGCATCT
    GTGTGCTTCT CGAAGCATGC CAGCAGCAAC AGCAGTCCCC ACCTGCAACA GGCCAGGTCT
    AGGGGCTATC TGGATGCCCC TGTGGTCTGT CCTTGCTTTC GTCGACACTG TGAACTAGGT
    CAGAGCTGGC CACAAATCCA CATCAGGACT GCAGCAGCAA AGATACCTCC CACAGGCTCA
    CAGCACAATG ACTTCCCTCT GTGGATCAGG ACAAAGAAGG GGCCTGCTCC GAGGTCCACA
    GTGGGCACAG ACTTGTTCTG GGTTCATCCC TTCCTTGACA TAGCCTGTCA ACCTCGATTG
    GGCACACCTT ATCTCTTAGA CACCAAGTGT CACCAGAGTA CCCCAGAAAA TCTTGGTGCC
    AATGCCAAGC CACCTCAAGG GTACCTCTGC TGGCAGGTAA AGACTCTGGT CAGGGCCCAT
    TCTATAGCTT CATTTCCTTT AGGTTGTCCT AAAGAGCCTC TGAAGCAGAA AGGTACAAAT
    TGGCCCTACT TGCGGGAAGG GACAGAAATA GAACCCGACC ACCCAATCCT TCACAAAACC
    CAGTCTCCTG GGCCCACCCA GCTGTCTCGT TTACTTCCTA CTTCTCAAAG CAGGGCCCCT
    CCTTTGCAGG GTGAGTGCAA GCTGAGTGAT TGTAGCTTTA AGAGGGCATC TCCAGACACA
    CCCTGTGTAG GCCTTCCACT GGAAAACCAG GTAGAACAGG CTTCTAGGTC ATGCCCAGCC
    CCTTCCCACC TTACTTCAGA ATTGCTGAAG CTCAACAGCC AAGAGGAAGT GCCCAGGCCG
    GCTGAATGCA AGCCAGGGCA CAGCCCAGAA AGCAGACTTG AGGAGCCCAA AGGTGTCCAC
    CCTGATGATG TTATCAGTAA ACAACAGTGC CTGCTAAACA TCTCCGGAGA GGCAGGAAGC
    CAGACGGAGA ACTACCCATC AATGAGTGCT CTCCAGGAGG AGAGAAAGCC GCCTGATAGG
    GCCTCCCTCT TCCCACAACA CTGCTGCCAT GGTGAGGTAC CTGTGTGCAA CAGAATGCTT
    GCAGGTTCCT GGGAAGAAGA GAGCGATAGC GCCTGTGCCC CAAGGACAGC CCTGAAATCA
    AGTGTCATTG GCTCTTTAGA AGCAGCAGAA GAGGGAGTGG TTCTAGACCT CCAACGCAAT
    GGGAAGGCCT TGCAAAGTCT TTTGGACTTC CCAACATTGG AAGTATCTCA CCGCAACCCT
    GGGAGTGAGT GTGCACAGGG ACCTGGGGAT GACCCGGAGC CTTCCCCTTC TGGCCAGCGG
    CAGGCTCCAG GAGCCCACGG GGCCTCCAGG CACAGCTGCA AATTCATTAT GATAGCAGTA
    AAGCAGGAAG GAAGGCAGAC TTCTACAAGG AAGGCTCACT GTCTCATAGA ACCACAGTGT
    GGGCACCTTC CAGAGAAGGG GACAAGCGCC ACGGCTTATG AGTCTTCCAG GGCCTCAGAA
    ATGCCCGTGT TTGGCAATGA ATGTGAGCTG CTGGGATGTC CAGGGGCAGA GGTGGATGGT
    GGGGCTCTTC AGCTGCTGGC CCAAGGCATA GAGCACGTGA GAGTGCCAGT CTCTAGGACT
    TCTTCGGATG ACACTTTGAG CTCAGAGGAG ACACTGGAGG GCGAGCTGCT TGGGGAAAGA
    TTGGACTTAG GGTCTGAGTG TGTACACACC GAGCAGAACC AGGAGTTGCC CAGCTCAGAT
    CGCAAACGAG GAAGGTGTGG CACAGGCGAT GCGGAGAATG CGGAAATCTG CGCCTGCGCG
    GGCGAGGTAG AGGGTCCCCT TTCAAGAGTC TTGGCTTCAA GCACCACAAC ACTGGAGTTC
    CAGGAACCAT GCGGTGGGTT TCAGCAGAGT GTGCTGGAGT CAGAGCAGCA CCCTGCCTGT
    CTTCAGATGA CCCCTTTAAG AACTGACATG CCCTGGTCCA GGGAGATGCC TGGCGGGGAC
    ATACCCTACA GCCAGGCCTT AACCTCAGAG GACTTGAACC CAGTTTGCCC TAGCCCCGAA
    GAAAGGGTCC CCCACAGTCC CTGCCAGGGG GAACCACCTC TGCACCACGT GCACCAGAGC
    GTATCTCTGG AGACCAAATC TGGTGTGATT CAAGTAGGGA CATCTGGGGA CCTCAGCGGT
    GAAGGACCTT CCCCAGAAAG CTACAACATC AAGAGACTGA AAACAACAGA AAGCAAGATC
    AAAGCCAGGC TGGCCTTAGC CCATAAGACC TTTACCAATT TTTTTGAAGC CAAAGTTATG
    GAGAAAACAA ATACTGGCTC TGTCAAAGGC GAGAAAGAGA AGCGCAGGCG AGCCCGGGGC
    TCCTGGCGTG CGTTTCTGAG AAGCAAAGAT GCGGAAGGCT CCAAACGACC TGCTTTAGTG
    AGTGGGGTTC CAGAACCAGG CTTTCCCCAA CTCCTCTGCT CTTCCCCACC AGCCACCAGA
    GGCCACTGTG AGAAGTGGAC GATTGACACC GACAGCCATG TATTTGGGGA GCACTGGACA
    CCCCTAAACT CCCCTGCAAC TCTGTCCTCC AGCCATCTGG CTTCTCCGGA ACACCGGAGG
    AGAAGTGAAC CGACCATAAG ACGCACTGCT GCCCAGGAAA GCAGTACGCA CCTCTCTTCT
    GGAGTCTTCC CAAAGTCTTC CTGGCTGACC TCTGCCACCA GCCCCGGAGC AGCGCAGATT
    GACATCAGTT TTGCCCTCCC CTGCAGCTCT GCCTGCCATT TGACCTATGA GAACCAAGGC
    ATGCCCTGCA GACCCACAAG CCCCAAACCC AGGAGTCCCA TGGCTGGGGC GCAGAGGATG
    GATCTCTGCC TTAGAGGCAG GACTAGTGCT GTCTCCATGG TCTCTCTCAG AAGTGACAAG
    GACGCCAACA GAAGTTCAGA AGTCCCCGAA CGGCCCAAGG CATCCAAGGC CAGGGCGAGC
    CTCTTACTCT CTCTGCAAAC ACTAAACCAG AACGACCAGA AGGAAGACAA GAGCCAGTGC
    CAGTGTTACC ATGGCCTGGG CACAGTGCCC TTCCTTAGGG ACCATCCTGG GAATGAGAGC
    TACATCGCCC AGGAAAGCCC GCCAGACAAG AAGCGCAATT GTTCCCATGA TCAGAAAACT
    TACACAGAGC CTCCTCACAG GCCGGTATCT GCCACTGAAG TGACACGGAC CCTCCCCTCC
    ATGTCCGCAG AGCAAGTCCC CAGAGAGGTC CCTTTACAGT TCAGGAGGGC CCCTCAACAC
    TCACACTTCA GCTTCGATGA CATATGGCTC GAAAAGGCTC AGAGGAGGAA GCTGAAGAAA
    CAGATGCTGC TTAGACGACA GATGTGCGCA GAGTTAGTGC CTGAGGACCC AGTTCAGTGC
    TGGAGGAAGA CAACCATCAC CTCTCCAGAG TCTCCGCATC TTCCTAGAAG AAGCCATCTG
    CTCTCCCAGA GTGCCCCAAT GGGACTGGAC CACACAGGCT GGCCAGAGCC CACGTCTGAC
    ACTGGGATGC CTGACGGAAC TCTGGACACG GTGTGCGCCG AGGAGACAGG CAGTGAGGAG
    GACCTGTATG AAGAGCTTCA CAACTCTGGC CACCACTACA GCCACCCTAG AGGAGGTGGA
    GAGCAACTGG CCATCAATGA GCTCATCAGT GATGGCAGTG TGGTGTGTGC TGAAGCCCTG
    TGGGACCATG TCACCATGGA TGACCAGGAG CTGGGCTTCA AAGCTGGGGA TGTCATCGAA
    GTGATGGATG CCACCAACAG GGAATGGTGG TGGGGCCGAG TGGCAGATGG CGAGGGGTGG
    TTCCCTGCCA GCTTTGTACG GCTGCGCGTG AACCAGGATG AGCCCGCGGA TGACTATGAG
    GCCCCGCGGG CCGGGGCTAG AGAGGCCGAT GACAGCGGTC CAGAGGCTCA GAGCTGCAAG
    GACCAGATGC GCACAAATGT CATCAATGAG ATCCTCAGCA CTGAGCGAGA CTACATCAAG
    CACCTTCGCG ACATCTGCGA GGGCTATGTC CGCCAGTGCC GCAAACGGGA GGATATGTTC
    AGTGAAGAGC AGCTGCGCAC CATCTTTGGG AACATCGAGG ACATCTACCG CTGCCAAAAG
    GCATTTGTGA AGGCTTTGGA GCAGAAGTTC AACACGGAGC GGCCACACCT GAGCGAGTTG
    GGGGCCTGCT TTCTGGAACA CCAAGCAGAC TTCCAGATCT ATTCTGAGTA CTGCAACAAC
    CACCCCAATG CCTGCGTGGA GCTCTCCCGG CTCACGAAGC TCAGCAAGTA CGTGTACTTC
    TTCGAGGCCT GCCGGCTGCT GCAAAGGATG ATTGACATCT CGCTGGATGG CTTTCTGCTG
    ACCCCGGTGC AGAAGATCTG CAAGTATCCC CTCCAGCTGG CGGAGCTGCT CAAGTACACA
    CACCCCCAGC ACAGGGACTT TAAGAATGTT GAAGCTGCCT TGCACGCCAT GAAGAATGTG
    GCCCAGCTCA TCAACGAACG GAAACGAAGA CTTGAAAACA TCGACAAGAT TGCTCAGTGG
    CAGAGCTCCA TAGAGGACTG GGAGGGGGAA GACCTTCTGG TCAGGAGCTC GGAACTCATC
    CACTCGGGGG AGCTGACCCG CGTGACACAG CCTCAAGCCA GGAGTCAGCA GAGAATGTTT
    TTTCTCTTTG ACCGCCAGCT CATCTACTGT AAAAAGGATC TGCTTCGCCG GGACGTGCTG
    TACTACAAGG GCCGGCTGGA CATGGATGAC CTGGAGGTGG TAGATGTGGA AGATGGGAAG
    GACCGAGACC TCCATGTGAG TGTCAAGAAC GCCTTCCGTC TGTACTGTGG TACCACGGGG
    GACAGCCACC TACTGTGTGC CAGGAAGCCA GAACAGAAGC AGCGCTGGCT GAAGGCCTTT
    GCCAGAGAGA GGGAGCAGGT GCGGCTGGAC CAGGAGACAG GCTTCTCCAT CACCGAGCTG
    CAGAGGAAGC AGGCCATGCT GAACGCCAGC AAGCAGCAGG CCACGGGGAA GCCTAAAGCT
    GTGGGAAGGC CAGGATACCT GACCCGCCAC AAGCATCCAT CATTGCCTGC CAGCCGGCCC
    CAGCAGCAAG TGTTGGTGCT AGCAGAGCCC AGGCGGAAGC CATCTAACTT CTGGCACAGC
    ATCAGCAGAC TGGCGCCTTT CCGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109461.1
    CDS114..5663
    Translation

    Target ORF information:

    RefSeq Version XM_030253601.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253601.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGATGTGC TGGATCTGGA GACTCCAGAG CCAGGTGTGC CCCCTCCAGG TGAGGAAGAG 
    TGTGAAGACA ACCAGCTCCA CCCAGGCCCT GCAGACTCGG AGGAACAGGA AACAAACCCC
    GAGGACTTGG AGACACTGTC CCATAAGAGC GAAAGTCGAT CCGGATCCGA TACAAAAACA
    GATCCCTTTG AAAGTGCTTC TGAGGCGGAG TCCCTCCCGG GTGACCTCAC TGGTGGAGTT
    GGCCTCTCCG AAAGAGAAGC AGGCTGGGGA TGCCCTGAGG ACTCAACAGT TAGACTTCCC
    CAAGGGCGGC ATTTGGTCTG TGACGAAGGG CTCCATACAG AGGAAGAGCC GGATCTACCC
    TCAGGCTTAA AGGCAGGTTC TCAGCTAAGT AAATTGCACA TGTTGACTGC TGGAAGACGG
    GATGAAGCAT GCCCCTGCAG CCGTGTGGCT AGCTTTGTAC AGGAGTCTGT GCCAGCATCT
    GTGTGCTTCT CGAAGCATGC CAGCAGCAAC AGCAGTCCCC ACCTGCAACA GGCCAGGTCT
    AGGGGCTATC TGGATGCCCC TGTGGTCTGT CCTTGCTTTC GTCGACACTG TGAACTAGGT
    CAGAGCTGGC CACAAATCCA CATCAGGACT GCAGCAGCAA AGATACCTCC CACAGGCTCA
    CAGCACAATG ACTTCCCTCT GTGGATCAGG ACAAAGAAGG GGCCTGCTCC GAGGTCCACA
    GTGGGCACAG ACTTGTTCTG GGTTCATCCC TTCCTTGACA TAGCCTGTCA ACCTCGATTG
    GGCACACCTT ATCTCTTAGA CACCAAGTGT CACCAGAGTA CCCCAGAAAA TCTTGGTGCC
    AATGCCAAGC CACCTCAAGG GTACCTCTGC TGGCAGGTAA AGACTCTGGT CAGGGCCCAT
    TCTATAGCTT CATTTCCTTT AGGTTGTCCT AAAGAGCCTC TGAAGCAGAA AGGTACAAAT
    TGGCCCTACT TGCGGGAAGG GACAGAAATA GAACCCGACC ACCCAATCCT TCACAAAACC
    CAGTCTCCTG GGCCCACCCA GCTGTCTCGT TTACTTCCTA CTTCTCAAAG CAGGGCCCCT
    CCTTTGCAGG GTGAGTGCAA GCTGAGTGAT TGTAGCTTTA AGAGGGCATC TCCAGACACA
    CCCTGTGTAG GCCTTCCACT GGAAAACCAG GTAGAACAGG CTTCTAGGTC ATGCCCAGCC
    CCTTCCCACC TTACTTCAGA ATTGCTGAAG CTCAACAGCC AAGAGGAAGT GCCCAGGCCG
    GCTGAATGCA AGCCAGGGCA CAGCCCAGAA AGCAGACTTG AGGAGCCCAA AGGTGTCCAC
    CCTGATGATG TTATCAGTAA ACAACAGTGC CTGCTAAACA TCTCCGGAGA GGCAGGAAGC
    CAGACGGAGA ACTACCCATC AATGAGTGCT CTCCAGGAGG AGAGAAAGCC GCCTGATAGG
    GCCTCCCTCT TCCCACAACA CTGCTGCCAT GGTGAGGTAC CTGTGTGCAA CAGAATGCTT
    GCAGGTTCCT GGGAAGAAGA GAGCGATAGC GCCTGTGCCC CAAGGACAGC CCTGAAATCA
    AGTGTCATTG GCTCTTTAGA AGCAGCAGAA GAGGGAGTGG TTCTAGACCT CCAACGCAAT
    GGGAAGGCCT TGCAAAGTCT TTTGGACTTC CCAACATTGG AAGTATCTCA CCGCAACCCT
    GGGAGTGAGT GTGCACAGGG ACCTGGGGAT GACCCGGAGC CTTCCCCTTC TGGCCAGCGG
    CAGGCTCCAG GAGCCCACGG GGCCTCCAGG CACAGCTGCA AATTCATTAT GATAGCAGTA
    AAGCAGGAAG GAAGGCAGAC TTCTACAAGG AAGGCTCACT GTCTCATAGA ACCACAGTGT
    GGGCACCTTC CAGAGAAGGG GACAAGCGCC ACGGCTTATG AGTCTTCCAG GGCCTCAGAA
    ATGCCCGTGT TTGGCAATGA ATGTGAGCTG CTGGGATGTC CAGGGGCAGA GGTGGATGGT
    GGGGCTCTTC AGCTGCTGGC CCAAGGCATA GAGCACGTGA GAGTGCCAGT CTCTAGGACT
    TCTTCGGATG ACACTTTGAG CTCAGAGGAG ACACTGGAGG GCGAGCTGCT TGGGGAAAGA
    TTGGACTTAG GGTCTGAGTG TGTACACACC GAGCAGAACC AGGAGTTGCC CAGCTCAGAT
    CGCAAACGAG GAAGGTGTGG CACAGGCGAT GCGGAGAATG CGGAAATCTG CGCCTGCGCG
    GGCGAGGTAG AGGGTCCCCT TTCAAGAGTC TTGGCTTCAA GCACCACAAC ACTGGAGTTC
    CAGGAACCAT GCGGTGGGTT TCAGCAGAGT GTGCTGGAGT CAGAGCAGCA CCCTGCCTGT
    CTTCAGATGA CCCCTTTAAG AACTGACATG CCCTGGTCCA GGGAGATGCC TGGCGGGGAC
    ATACCCTACA GCCAGGCCTT AACCTCAGAG GACTTGAACC CAGTTTGCCC TAGCCCCGAA
    GAAAGGGTCC CCCACAGTCC CTGCCAGGGG GAACCACCTC TGCACCACGT GCACCAGAGC
    GTATCTCTGG AGACCAAATC TGGTGTGATT CAAGTAGGGA CATCTGGGGA CCTCAGCGGT
    GAAGGACCTT CCCCAGAAAG CTACAACATC AAGAGACTGA AAACAACAGA AAGCAAGATC
    AAAGCCAGGC TGGCCTTAGC CCATAAGACC TTTACCAATT TTTTTGAAGC CAAAGTTATG
    GAGAAAACAA ATACTGGCTC TGTCAAAGGC GAGAAAGAGA AGCGCAGGCG AGCCCGGGGC
    TCCTGGCGTG CGTTTCTGAG AAGCAAAGAT GCGGAAGGCT CCAAACGACC TGCTTTAGTG
    AGTGGGGTTC CAGAACCAGG CTTTCCCCAA CTCCTCTGCT CTTCCCCACC AGCCACCAGA
    GGCCACTGTG AGAAGTGGAC GATTGACACC GACAGCCATG TATTTGGGGA GCACTGGACA
    CCCCTAAACT CCCCTGCAAC TCTGTCCTCC AGCCATCTGG CTTCTCCGGA ACACCGGAGG
    AGAAGTGAAC CGACCATAAG ACGCACTGCT GCCCAGGAAA GCAGTACGCA CCTCTCTTCT
    GGAGTCTTCC CAAAGTCTTC CTGGCTGACC TCTGCCACCA GCCCCGGAGC AGCGCAGATT
    GACATCAGTT TTGCCCTCCC CTGCAGCTCT GCCTGCCATT TGACCTATGA GAACCAAGGC
    ATGCCCTGCA GACCCACAAG CCCCAAACCC AGGAGTCCCA TGGCTGGGGC GCAGAGGATG
    GATCTCTGCC TTAGAGGCAG GACTAGTGCT GTCTCCATGG TCTCTCTCAG AAGTGACAAG
    GACGCCAACA GAAGTTCAGA AGTCCCCGAA CGGCCCAAGG CATCCAAGGC CAGGGCGAGC
    CTCTTACTCT CTCTGCAAAC ACTAAACCAG AACGACCAGA AGGAAGACAA GAGCCAGTGC
    CAGTGTTACC ATGGCCTGGG CACAGTGCCC TTCCTTAGGG ACCATCCTGG GAATGAGAGC
    TACATCGCCC AGGAAAGCCC GCCAGACAAG AAGCGCAATT GTTCCCATGA TCAGAAAACT
    TACACAGAGC CTCCTCACAG GCCGGTATCT GCCACTGAAG TGACACGGAC CCTCCCCTCC
    ATGTCCGCAG AGCAAGTCCC CAGAGAGGTC CCTTTACAGT TCAGGAGGGC CCCTCAACAC
    TCACACTTCA GCTTCGATGA CATATGGCTC GAAAAGGCTC AGAGGAGGAA GCTGAAGAAA
    CAGATGCTGC TTAGACGACA GATGTGCGCA GAGTTAGTGC CTGAGGACCC AGTTCAGTGC
    TGGAGGAAGA CAACCATCAC CTCTCCAGAG TCTCCGCATC TTCCTAGAAG AAGCCATCTG
    CTCTCCCAGA GTGCCCCAAT GGGACTGGAC CACACAGGCT GGCCAGAGCC CACGTCTGAC
    ACTGGGATGC CTGACGGAAC TCTGGACACG GTGTGCGCCG AGGAGACAGG CAGTGAGGAG
    GACCTGTATG AAGAGCTTCA CAACTCTGGC CACCACTACA GCCACCCTAG AGGAGGTGGA
    GAGCAACTGG CCATCAATGA GCTCATCAGT GATGGCAGTG TGGTGTGTGC TGAAGCCCTG
    TGGGACCATG TCACCATGGA TGACCAGGAG CTGGGCTTCA AAGCTGGGGA TGTCATCGAA
    GTGATGGATG CCACCAACAG GGAATGGTGG TGGGGCCGAG TGGCAGATGG CGAGGGGTGG
    TTCCCTGCCA GCTTTGTACG GCTGCGCGTG AACCAGGATG AGCCCGCGGA TGACTATGAG
    GCCCCGCGGG CCGGGGCTAG AGAGGCCGAT GACAGCGGTC CAGAGGCTCA GAGCTGCAAG
    GACCAGATGC GCACAAATGT CATCAATGAG ATCCTCAGCA CTGAGCGAGA CTACATCAAG
    CACCTTCGCG ACATCTGCGA GGGCTATGTC CGCCAGTGCC GCAAACGGGA GGATATGTTC
    AGTGAAGAGC AGCTGCGCAC CATCTTTGGG AACATCGAGG ACATCTACCG CTGCCAAAAG
    GCATTTGTGA AGGCTTTGGA GCAGAAGTTC AACACGGAGC GGCCACACCT GAGCGAGTTG
    GGGGCCTGCT TTCTGGAACA CCAAGCAGAC TTCCAGATCT ATTCTGAGTA CTGCAACAAC
    CACCCCAATG CCTGCGTGGA GCTCTCCCGG CTCACGAAGC TCAGCAAGTA CGTGTACTTC
    TTCGAGGCCT GCCGGCTGCT GCAAAGGATG ATTGACATCT CGCTGGATGG CTTTCTGCTG
    ACCCCGGTGC AGAAGATCTG CAAGTATCCC CTCCAGCTGG CGGAGCTGCT CAAGTACACA
    CACCCCCAGC ACAGGGACTT TAAGAATGTT GAAGCTGCCT TGCACGCCAT GAAGAATGTG
    GCCCAGCTCA TCAACGAACG GAAACGAAGA CTTGAAAACA TCGACAAGAT TGCTCAGTGG
    CAGAGCTCCA TAGAGGACTG GGAGGGGGAA GACCTTCTGG TCAGGAGCTC GGAACTCATC
    CACTCGGGGG AGCTGACCCG CGTGACACAG CCTCAAGCCA GGAGTCAGCA GAGAATGTTT
    TTTCTCTTTG ACCGCCAGCT CATCTACTGT AAAAAGGATC TGCTTCGCCG GGACGTGCTG
    TACTACAAGG GCCGGCTGGA CATGGATGAC CTGGAGGTGG TAGATGTGGA AGATGGGAAG
    GACCGAGACC TCCATGTGAG TGTCAAGAAC GCCTTCCGTC TGTACTGTGG TACCACGGGG
    GACAGCCACC TACTGTGTGC CAGGAAGCCA GAACAGAAGC AGCGCTGGCT GAAGGCCTTT
    GCCAGAGAGA GGGAGCAGGT GCGGCTGGAC CAGGAGACAG GCTTCTCCAT CACCGAGCTG
    CAGAGGAAGC AGGCCATGCT GAACGCCAGC AAGCAGCAGG CCACGGGGAA GCCTAAAGCT
    GTGGGAAGGC CAGGATACCT GACCCGCCAC AAGCATCCAT CATTGCCTGC CAGCCGGCCC
    CAGCAGCAAG TGTTGGTGCT AGCAGAGCCC AGGCGGAAGC CATCTAACTT CTGGCACAGC
    ATCAGCAGAC TGGCGCCTTT CCGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85136
    Clone ID Related Accession (Same CDS sequence) XM_030253603.1
    Accession Version XM_030253603.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGTG TGGTGCACTT CCTTCGCAGC TTCTTTAAGA CTCCAGAGCC AGGTGTGCCC 
    CCTCCAGGTG AGGAAGAGTG TGAAGACAAC CAGCTCCACC CAGGCCCTGC AGACTCGGAG
    GAACAGGAAA CAAACCCCGA GGACTTGGAG ACACTGTCCC ATAAGAGCGA AAGTCGATCC
    GGATCCGATA CAAAAACAGA TCCCTTTGAA AGTGCTTCTG AGGCGGAGTC CCTCCCGGGT
    GACCTCACTG GTGGAGTTGG CCTCTCCGAA AGAGAAGCAG GCTGGGGATG CCCTGAGGAC
    TCAACAGTTA GACTTCCCCA AGGGCGGCAT TTGGTCTGTG ACGAAGGGCT CCATACAGAG
    GAAGAGCCGG ATCTACCCTC AGGCTTAAAG GCAGGTTCTC AGCTAAGTAA ATTGCACATG
    TTGACTGCTG GAAGACGGGA TGAAGCATGC CCCTGCAGCC GTGTGGCTAG CTTTGTACAG
    GAGTCTGTGC CAGCATCTGT GTGCTTCTCG AAGCATGCCA GCAGCAACAG CAGTCCCCAC
    CTGCAACAGG CCAGGTCTAG GGGCTATCTG GATGCCCCTG TGGTCTGTCC TTGCTTTCGT
    CGACACTGTG AACTAGGTCA GAGCTGGCCA CAAATCCACA TCAGGACTGC AGCAGCAAAG
    ATACCTCCCA CAGGCTCACA GCACAATGAC TTCCCTCTGT GGATCAGGAC AAAGAAGGGG
    CCTGCTCCGA GGTCCACAGT GGGCACAGAC TTGTTCTGGG TTCATCCCTT CCTTGACATA
    GCCTGTCAAC CTCGATTGGG CACACCTTAT CTCTTAGACA CCAAGTGTCA CCAGAGTACC
    CCAGAAAATC TTGGTGCCAA TGCCAAGCCA CCTCAAGGGT ACCTCTGCTG GCAGGTAAAG
    ACTCTGGTCA GGGCCCATTC TATAGCTTCA TTTCCTTTAG GTTGTCCTAA AGAGCCTCTG
    AAGCAGAAAG GTACAAATTG GCCCTACTTG CGGGAAGGGA CAGAAATAGA ACCCGACCAC
    CCAATCCTTC ACAAAACCCA GTCTCCTGGG CCCACCCAGC TGTCTCGTTT ACTTCCTACT
    TCTCAAAGCA GGGCCCCTCC TTTGCAGGGT GAGTGCAAGC TGAGTGATTG TAGCTTTAAG
    AGGGCATCTC CAGACACACC CTGTGTAGGC CTTCCACTGG AAAACCAGGT AGAACAGGCT
    TCTAGGTCAT GCCCAGCCCC TTCCCACCTT ACTTCAGAAT TGCTGAAGCT CAACAGCCAA
    GAGGAAGTGC CCAGGCCGGC TGAATGCAAG CCAGGGCACA GCCCAGAAAG CAGACTTGAG
    GAGCCCAAAG GTGTCCACCC TGATGATGTT ATCAGTAAAC AACAGTGCCT GCTAAACATC
    TCCGGAGAGG CAGGAAGCCA GACGGAGAAC TACCCATCAA TGAGTGCTCT CCAGGAGGAG
    AGAAAGCCGC CTGATAGGGC CTCCCTCTTC CCACAACACT GCTGCCATGG TGAGGTACCT
    GTGTGCAACA GAATGCTTGC AGGTTCCTGG GAAGAAGAGA GCGATAGCGC CTGTGCCCCA
    AGGACAGCCC TGAAATCAAG TGTCATTGGC TCTTTAGAAG CAGCAGAAGA GGGAGTGGTT
    CTAGACCTCC AACGCAATGG GAAGGCCTTG CAAAGTCTTT TGGACTTCCC AACATTGGAA
    GTATCTCACC GCAACCCTGG GAGTGAGTGT GCACAGGGAC CTGGGGATGA CCCGGAGCCT
    TCCCCTTCTG GCCAGCGGCA GGCTCCAGGA GCCCACGGGG CCTCCAGGCA CAGCTGCAAA
    TTCATTATGA TAGCAGTAAA GCAGGAAGGA AGGCAGACTT CTACAAGGAA GGCTCACTGT
    CTCATAGAAC CACAGTGTGG GCACCTTCCA GAGAAGGGGA CAAGCGCCAC GGCTTATGAG
    TCTTCCAGGG CCTCAGAAAT GCCCGTGTTT GGCAATGAAT GTGAGCTGCT GGGATGTCCA
    GGGGCAGAGG TGGATGGTGG GGCTCTTCAG CTGCTGGCCC AAGGCATAGA GCACGTGAGA
    GTGCCAGTCT CTAGGACTTC TTCGGATGAC ACTTTGAGCT CAGAGGAGAC ACTGGAGGGC
    GAGCTGCTTG GGGAAAGATT GGACTTAGGG TCTGAGTGTG TACACACCGA GCAGAACCAG
    GAGTTGCCCA GCTCAGATCG CAAACGAGGA AGGTGTGGCA CAGGCGATGC GGAGAATGCG
    GAAATCTGCG CCTGCGCGGG CGAGGTAGAG GGTCCCCTTT CAAGAGTCTT GGCTTCAAGC
    ACCACAACAC TGGAGTTCCA GGAACCATGC GGTGGGTTTC AGCAGAGTGT GCTGGAGTCA
    GAGCAGCACC CTGCCTGTCT TCAGATGACC CCTTTAAGAA CTGACATGCC CTGGTCCAGG
    GAGATGCCTG GCGGGGACAT ACCCTACAGC CAGGCCTTAA CCTCAGAGGA CTTGAACCCA
    GTTTGCCCTA GCCCCGAAGA AAGGGTCCCC CACAGTCCCT GCCAGGGGGA ACCACCTCTG
    CACCACGTGC ACCAGAGCGT ATCTCTGGAG ACCAAATCTG GTGTGATTCA AGTAGGGACA
    TCTGGGGACC TCAGCGGTGA AGGACCTTCC CCAGAAAGCT ACAACATCAA GAGACTGAAA
    ACAACAGAAA GCAAGATCAA AGCCAGGCTG GCCTTAGCCC ATAAGACCTT TACCAATTTT
    TTTGAAGCCA AAGTTATGGA GAAAACAAAT ACTGGCTCTG TCAAAGGCGA GAAAGAGAAG
    CGCAGGCGAG CCCGGGGCTC CTGGCGTGCG TTTCTGAGAA GCAAAGATGC GGAAGGCTCC
    AAACGACCTG CTTTAGTGAG TGGGGTTCCA GAACCAGGCT TTCCCCAACT CCTCTGCTCT
    TCCCCACCAG CCACCAGAGG CCACTGTGAG AAGTGGACGA TTGACACCGA CAGCCATGTA
    TTTGGGGAGC ACTGGACACC CCTAAACTCC CCTGCAACTC TGTCCTCCAG CCATCTGGCT
    TCTCCGGAAC ACCGGAGGAG AAGTGAACCG ACCATAAGAC GCACTGCTGC CCAGGAAAGC
    AGTACGCACC TCTCTTCTGG AGTCTTCCCA AAGTCTTCCT GGCTGACCTC TGCCACCAGC
    CCCGGAGCAG CGCAGATTGA CATCAGTTTT GCCCTCCCCT GCAGCTCTGC CTGCCATTTG
    ACCTATGAGA ACCAAGGCAT GCCCTGCAGA CCCACAAGCC CCAAACCCAG GAGTCCCATG
    GCTGGGGCGC AGAGGATGGA TCTCTGCCTT AGAGGCAGGA CTAGTGCTGT CTCCATGGTC
    TCTCTCAGAA GTGACAAGGA CGCCAACAGA AGTTCAGAAG TCCCCGAACG GCCCAAGGCA
    TCCAAGGCCA GGGCGAGCCT CTTACTCTCT CTGCAAACAC TAAACCAGAA CGACCAGAAG
    GAAGACAAGA GCCAGTGCCA GTGTTACCAT GGCCTGGGCA CAGTGCCCTT CCTTAGGGAC
    CATCCTGGGA ATGAGAGCTA CATCGCCCAG GAAAGCCCGC CAGACAAGAA GCGCAATTGT
    TCCCATGATC AGAAAACTTA CACAGAGCCT CCTCACAGGC CGGTATCTGC CACTGAAGTG
    ACACGGACCC TCCCCTCCAT GTCCGCAGAG CAAGTCCCCA GAGAGGTCCC TTTACAGTTC
    AGGAGGGCCC CTCAACACTC ACACTTCAGC TTCGATGACA TATGGCTCGA AAAGGCTCAG
    AGGAGGAAGC TGAAGAAACA GATGCTGCTT AGACGACAGA TGTGCGCAGA GTTAGTGCCT
    GAGGACCCAG TTCAGTGCTG GAGGAAGACA ACCATCACCT CTCCAGAGTC TCCGCATCTT
    CCTAGAAGAA GCCATCTGCT CTCCCAGAGT GCCCCAATGG GACTGGACCA CACAGGCTGG
    CCAGAGCCCA CGTCTGACAC TGGGATGCCT GACGGAACTC TGGACACGGT GTGCGCCGAG
    GAGACAGGCA GTGAGGAGGA CCTGTATGAA GAGCTTCACA ACTCTGGCCA CCACTACAGC
    CACCCTAGAG GAGGTGGAGA GCAACTGGCC ATCAATGAGC TCATCAGTGA TGGCAGTGTG
    GTGTGTGCTG AAGCCCTGTG GGACCATGTC ACCATGGATG ACCAGGAGCT GGGCTTCAAA
    GCTGGGGATG TCATCGAAGT GATGGATGCC ACCAACAGGG AATGGTGGTG GGGCCGAGTG
    GCAGATGGCG AGGGGTGGTT CCCTGCCAGC TTTGTACGGC TGCGCGTGAA CCAGGATGAG
    CCCGCGGATG ACTATGAGGC CCCGCGGGCC GGGGCTAGAG AGGCCGATGA CAGCGGTCCA
    GAGGCTCAGA GCTGCAAGGA CCAGATGCGC ACAAATGTCA TCAATGAGAT CCTCAGCACT
    GAGCGAGACT ACATCAAGCA CCTTCGCGAC ATCTGCGAGG GCTATGTCCG CCAGTGCCGC
    AAACGGGAGG ATATGTTCAG TGAAGAGCAG CTGCGCACCA TCTTTGGGAA CATCGAGGAC
    ATCTACCGCT GCCAAAAGGC ATTTGTGAAG GCTTTGGAGC AGAAGTTCAA CACGGAGCGG
    CCACACCTGA GCGAGTTGGG GGCCTGCTTT CTGGAACACC AAGCAGACTT CCAGATCTAT
    TCTGAGTACT GCAACAACCA CCCCAATGCC TGCGTGGAGC TCTCCCGGCT CACGAAGCTC
    AGCAAGTACG TGTACTTCTT CGAGGCCTGC CGGCTGCTGC AAAGGATGAT TGACATCTCG
    CTGGATGGCT TTCTGCTGAC CCCGGTGCAG AAGATCTGCA AGTATCCCCT CCAGCTGGCG
    GAGCTGCTCA AGTACACACA CCCCCAGCAC AGGGACTTTA AGAATGTTGA AGCTGCCTTG
    CACGCCATGA AGAATGTGGC CCAGCTCATC AACGAACGGA AACGAAGACT TGAAAACATC
    GACAAGATTG CTCAGTGGCA GAGCTCCATA GAGGACTGGG AGGGGGAAGA CCTTCTGGTC
    AGGAGCTCGG AACTCATCCA CTCGGGGGAG CTGACCCGCG TGACACAGCC TCAAGCCAGG
    AGTCAGCAGA GAATGTTTTT TCTCTTTGAC CGCCAGCTCA TCTACTGTAA AAAGGATCTG
    CTTCGCCGGG ACGTGCTGTA CTACAAGGGC CGGCTGGACA TGGATGACCT GGAGGTGGTA
    GATGTGGAAG ATGGGAAGGA CCGAGACCTC CATGTGAGTG TCAAGAACGC CTTCCGTCTG
    TACTGTGGTA CCACGGGGGA CAGCCACCTA CTGTGTGCCA GGAAGCCAGA ACAGAAGCAG
    CGCTGGCTGA AGGCCTTTGC CAGAGAGAGG GAGCAGGCTT CTCCATCACC GAGCTGCAGA
    GGAAGCAGGC CATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109463.1
    CDS122..5479
    Translation

    Target ORF information:

    RefSeq Version XM_030253603.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253603.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGTG TGGTGCACTT CCTTCGCAGC TTCTTTAAGA CTCCAGAGCC AGGTGTGCCC 
    CCTCCAGGTG AGGAAGAGTG TGAAGACAAC CAGCTCCACC CAGGCCCTGC AGACTCGGAG
    GAACAGGAAA CAAACCCCGA GGACTTGGAG ACACTGTCCC ATAAGAGCGA AAGTCGATCC
    GGATCCGATA CAAAAACAGA TCCCTTTGAA AGTGCTTCTG AGGCGGAGTC CCTCCCGGGT
    GACCTCACTG GTGGAGTTGG CCTCTCCGAA AGAGAAGCAG GCTGGGGATG CCCTGAGGAC
    TCAACAGTTA GACTTCCCCA AGGGCGGCAT TTGGTCTGTG ACGAAGGGCT CCATACAGAG
    GAAGAGCCGG ATCTACCCTC AGGCTTAAAG GCAGGTTCTC AGCTAAGTAA ATTGCACATG
    TTGACTGCTG GAAGACGGGA TGAAGCATGC CCCTGCAGCC GTGTGGCTAG CTTTGTACAG
    GAGTCTGTGC CAGCATCTGT GTGCTTCTCG AAGCATGCCA GCAGCAACAG CAGTCCCCAC
    CTGCAACAGG CCAGGTCTAG GGGCTATCTG GATGCCCCTG TGGTCTGTCC TTGCTTTCGT
    CGACACTGTG AACTAGGTCA GAGCTGGCCA CAAATCCACA TCAGGACTGC AGCAGCAAAG
    ATACCTCCCA CAGGCTCACA GCACAATGAC TTCCCTCTGT GGATCAGGAC AAAGAAGGGG
    CCTGCTCCGA GGTCCACAGT GGGCACAGAC TTGTTCTGGG TTCATCCCTT CCTTGACATA
    GCCTGTCAAC CTCGATTGGG CACACCTTAT CTCTTAGACA CCAAGTGTCA CCAGAGTACC
    CCAGAAAATC TTGGTGCCAA TGCCAAGCCA CCTCAAGGGT ACCTCTGCTG GCAGGTAAAG
    ACTCTGGTCA GGGCCCATTC TATAGCTTCA TTTCCTTTAG GTTGTCCTAA AGAGCCTCTG
    AAGCAGAAAG GTACAAATTG GCCCTACTTG CGGGAAGGGA CAGAAATAGA ACCCGACCAC
    CCAATCCTTC ACAAAACCCA GTCTCCTGGG CCCACCCAGC TGTCTCGTTT ACTTCCTACT
    TCTCAAAGCA GGGCCCCTCC TTTGCAGGGT GAGTGCAAGC TGAGTGATTG TAGCTTTAAG
    AGGGCATCTC CAGACACACC CTGTGTAGGC CTTCCACTGG AAAACCAGGT AGAACAGGCT
    TCTAGGTCAT GCCCAGCCCC TTCCCACCTT ACTTCAGAAT TGCTGAAGCT CAACAGCCAA
    GAGGAAGTGC CCAGGCCGGC TGAATGCAAG CCAGGGCACA GCCCAGAAAG CAGACTTGAG
    GAGCCCAAAG GTGTCCACCC TGATGATGTT ATCAGTAAAC AACAGTGCCT GCTAAACATC
    TCCGGAGAGG CAGGAAGCCA GACGGAGAAC TACCCATCAA TGAGTGCTCT CCAGGAGGAG
    AGAAAGCCGC CTGATAGGGC CTCCCTCTTC CCACAACACT GCTGCCATGG TGAGGTACCT
    GTGTGCAACA GAATGCTTGC AGGTTCCTGG GAAGAAGAGA GCGATAGCGC CTGTGCCCCA
    AGGACAGCCC TGAAATCAAG TGTCATTGGC TCTTTAGAAG CAGCAGAAGA GGGAGTGGTT
    CTAGACCTCC AACGCAATGG GAAGGCCTTG CAAAGTCTTT TGGACTTCCC AACATTGGAA
    GTATCTCACC GCAACCCTGG GAGTGAGTGT GCACAGGGAC CTGGGGATGA CCCGGAGCCT
    TCCCCTTCTG GCCAGCGGCA GGCTCCAGGA GCCCACGGGG CCTCCAGGCA CAGCTGCAAA
    TTCATTATGA TAGCAGTAAA GCAGGAAGGA AGGCAGACTT CTACAAGGAA GGCTCACTGT
    CTCATAGAAC CACAGTGTGG GCACCTTCCA GAGAAGGGGA CAAGCGCCAC GGCTTATGAG
    TCTTCCAGGG CCTCAGAAAT GCCCGTGTTT GGCAATGAAT GTGAGCTGCT GGGATGTCCA
    GGGGCAGAGG TGGATGGTGG GGCTCTTCAG CTGCTGGCCC AAGGCATAGA GCACGTGAGA
    GTGCCAGTCT CTAGGACTTC TTCGGATGAC ACTTTGAGCT CAGAGGAGAC ACTGGAGGGC
    GAGCTGCTTG GGGAAAGATT GGACTTAGGG TCTGAGTGTG TACACACCGA GCAGAACCAG
    GAGTTGCCCA GCTCAGATCG CAAACGAGGA AGGTGTGGCA CAGGCGATGC GGAGAATGCG
    GAAATCTGCG CCTGCGCGGG CGAGGTAGAG GGTCCCCTTT CAAGAGTCTT GGCTTCAAGC
    ACCACAACAC TGGAGTTCCA GGAACCATGC GGTGGGTTTC AGCAGAGTGT GCTGGAGTCA
    GAGCAGCACC CTGCCTGTCT TCAGATGACC CCTTTAAGAA CTGACATGCC CTGGTCCAGG
    GAGATGCCTG GCGGGGACAT ACCCTACAGC CAGGCCTTAA CCTCAGAGGA CTTGAACCCA
    GTTTGCCCTA GCCCCGAAGA AAGGGTCCCC CACAGTCCCT GCCAGGGGGA ACCACCTCTG
    CACCACGTGC ACCAGAGCGT ATCTCTGGAG ACCAAATCTG GTGTGATTCA AGTAGGGACA
    TCTGGGGACC TCAGCGGTGA AGGACCTTCC CCAGAAAGCT ACAACATCAA GAGACTGAAA
    ACAACAGAAA GCAAGATCAA AGCCAGGCTG GCCTTAGCCC ATAAGACCTT TACCAATTTT
    TTTGAAGCCA AAGTTATGGA GAAAACAAAT ACTGGCTCTG TCAAAGGCGA GAAAGAGAAG
    CGCAGGCGAG CCCGGGGCTC CTGGCGTGCG TTTCTGAGAA GCAAAGATGC GGAAGGCTCC
    AAACGACCTG CTTTAGTGAG TGGGGTTCCA GAACCAGGCT TTCCCCAACT CCTCTGCTCT
    TCCCCACCAG CCACCAGAGG CCACTGTGAG AAGTGGACGA TTGACACCGA CAGCCATGTA
    TTTGGGGAGC ACTGGACACC CCTAAACTCC CCTGCAACTC TGTCCTCCAG CCATCTGGCT
    TCTCCGGAAC ACCGGAGGAG AAGTGAACCG ACCATAAGAC GCACTGCTGC CCAGGAAAGC
    AGTACGCACC TCTCTTCTGG AGTCTTCCCA AAGTCTTCCT GGCTGACCTC TGCCACCAGC
    CCCGGAGCAG CGCAGATTGA CATCAGTTTT GCCCTCCCCT GCAGCTCTGC CTGCCATTTG
    ACCTATGAGA ACCAAGGCAT GCCCTGCAGA CCCACAAGCC CCAAACCCAG GAGTCCCATG
    GCTGGGGCGC AGAGGATGGA TCTCTGCCTT AGAGGCAGGA CTAGTGCTGT CTCCATGGTC
    TCTCTCAGAA GTGACAAGGA CGCCAACAGA AGTTCAGAAG TCCCCGAACG GCCCAAGGCA
    TCCAAGGCCA GGGCGAGCCT CTTACTCTCT CTGCAAACAC TAAACCAGAA CGACCAGAAG
    GAAGACAAGA GCCAGTGCCA GTGTTACCAT GGCCTGGGCA CAGTGCCCTT CCTTAGGGAC
    CATCCTGGGA ATGAGAGCTA CATCGCCCAG GAAAGCCCGC CAGACAAGAA GCGCAATTGT
    TCCCATGATC AGAAAACTTA CACAGAGCCT CCTCACAGGC CGGTATCTGC CACTGAAGTG
    ACACGGACCC TCCCCTCCAT GTCCGCAGAG CAAGTCCCCA GAGAGGTCCC TTTACAGTTC
    AGGAGGGCCC CTCAACACTC ACACTTCAGC TTCGATGACA TATGGCTCGA AAAGGCTCAG
    AGGAGGAAGC TGAAGAAACA GATGCTGCTT AGACGACAGA TGTGCGCAGA GTTAGTGCCT
    GAGGACCCAG TTCAGTGCTG GAGGAAGACA ACCATCACCT CTCCAGAGTC TCCGCATCTT
    CCTAGAAGAA GCCATCTGCT CTCCCAGAGT GCCCCAATGG GACTGGACCA CACAGGCTGG
    CCAGAGCCCA CGTCTGACAC TGGGATGCCT GACGGAACTC TGGACACGGT GTGCGCCGAG
    GAGACAGGCA GTGAGGAGGA CCTGTATGAA GAGCTTCACA ACTCTGGCCA CCACTACAGC
    CACCCTAGAG GAGGTGGAGA GCAACTGGCC ATCAATGAGC TCATCAGTGA TGGCAGTGTG
    GTGTGTGCTG AAGCCCTGTG GGACCATGTC ACCATGGATG ACCAGGAGCT GGGCTTCAAA
    GCTGGGGATG TCATCGAAGT GATGGATGCC ACCAACAGGG AATGGTGGTG GGGCCGAGTG
    GCAGATGGCG AGGGGTGGTT CCCTGCCAGC TTTGTACGGC TGCGCGTGAA CCAGGATGAG
    CCCGCGGATG ACTATGAGGC CCCGCGGGCC GGGGCTAGAG AGGCCGATGA CAGCGGTCCA
    GAGGCTCAGA GCTGCAAGGA CCAGATGCGC ACAAATGTCA TCAATGAGAT CCTCAGCACT
    GAGCGAGACT ACATCAAGCA CCTTCGCGAC ATCTGCGAGG GCTATGTCCG CCAGTGCCGC
    AAACGGGAGG ATATGTTCAG TGAAGAGCAG CTGCGCACCA TCTTTGGGAA CATCGAGGAC
    ATCTACCGCT GCCAAAAGGC ATTTGTGAAG GCTTTGGAGC AGAAGTTCAA CACGGAGCGG
    CCACACCTGA GCGAGTTGGG GGCCTGCTTT CTGGAACACC AAGCAGACTT CCAGATCTAT
    TCTGAGTACT GCAACAACCA CCCCAATGCC TGCGTGGAGC TCTCCCGGCT CACGAAGCTC
    AGCAAGTACG TGTACTTCTT CGAGGCCTGC CGGCTGCTGC AAAGGATGAT TGACATCTCG
    CTGGATGGCT TTCTGCTGAC CCCGGTGCAG AAGATCTGCA AGTATCCCCT CCAGCTGGCG
    GAGCTGCTCA AGTACACACA CCCCCAGCAC AGGGACTTTA AGAATGTTGA AGCTGCCTTG
    CACGCCATGA AGAATGTGGC CCAGCTCATC AACGAACGGA AACGAAGACT TGAAAACATC
    GACAAGATTG CTCAGTGGCA GAGCTCCATA GAGGACTGGG AGGGGGAAGA CCTTCTGGTC
    AGGAGCTCGG AACTCATCCA CTCGGGGGAG CTGACCCGCG TGACACAGCC TCAAGCCAGG
    AGTCAGCAGA GAATGTTTTT TCTCTTTGAC CGCCAGCTCA TCTACTGTAA AAAGGATCTG
    CTTCGCCGGG ACGTGCTGTA CTACAAGGGC CGGCTGGACA TGGATGACCT GGAGGTGGTA
    GATGTGGAAG ATGGGAAGGA CCGAGACCTC CATGTGAGTG TCAAGAACGC CTTCCGTCTG
    TACTGTGGTA CCACGGGGGA CAGCCACCTA CTGTGTGCCA GGAAGCCAGA ACAGAAGCAG
    CGCTGGCTGA AGGCCTTTGC CAGAGAGAGG GAGCAGGCTT CTCCATCACC GAGCTGCAGA
    GGAAGCAGGC CATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85137
    Clone ID Related Accession (Same CDS sequence) XM_030253604.1
    Accession Version XM_030253604.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCTCGGTG TGGTGCACTT CCTTCGCAGC TTCTTTAAGA CTCCAGAGCC AGGTGTGCCC 
    CCTCCAGGTG AGGAAGAGTG TGAAGACAAC CAGCTCCACC CAGGCCCTGC AGACTCGGAG
    GAACAGGAAA CAAACCCCGA GGACTTGGAG ACACTGTCCC ATAAGAGCGA AAGTCGATCC
    GGATCCGATA CAAAAACAGA TCCCTTTGAA AGTGCTTCTG AGGCGGAGTC CCTCCCGGGT
    GACCTCACTG GTGGAGTTGG CCTCTCCGAA AGAGAAGCAG GCTGGGGATG CCCTGAGGAC
    TCAACAGTTA GACTTCCCCA AGGGCGGCAT TTGGTCTGTG ACGAAGGGCT CCATACAGAG
    GAAGAGCCGG ATCTACCCTC AGGCTTAAAG GCAGGTTCTC AGCTAAGTAA ATTGCACATG
    TTGACTGCTG GAAGACGGGA TGAAGCATGC CCCTGCAGCC GTGTGGCTAG CTTTGTACAG
    GAGTCTGTGC CAGCATCTGT GTGCTTCTCG AAGCATGCCA GCAGCAACAG CAGTCCCCAC
    CTGCAACAGG CCAGGTCTAG GGGCTATCTG GATGCCCCTG TGGTCTGTCC TTGCTTTCGT
    CGACACTGTG AACTAGGTCA GAGCTGGCCA CAAATCCACA TCAGGACTGC AGCAGCAAAG
    ATACCTCCCA CAGGCTCACA GCACAATGAC TTCCCTCTGT GGATCAGGAC AAAGAAGGGG
    CCTGCTCCGA GGTCCACAGT GGGCACAGAC TTGTTCTGGG TTCATCCCTT CCTTGACATA
    GCCTGTCAAC CTCGATTGGG CACACCTTAT CTCTTAGACA CCAAGTGTCA CCAGAGTACC
    CCAGAAAATC TTGGTGCCAA TGCCAAGCCA CCTCAAGGGT ACCTCTGCTG GCAGGTAAAG
    ACTCTGGTCA GGGCCCATTC TATAGCTTCA TTTCCTTTAG GTTGTCCTAA AGAGCCTCTG
    AAGCAGAAAG GTACAAATTG GCCCTACTTG CGGGAAGGGA CAGAAATAGA ACCCGACCAC
    CCAATCCTTC ACAAAACCCA GTCTCCTGGG CCCACCCAGC TGTCTCGTTT ACTTCCTACT
    TCTCAAAGCA GGGCCCCTCC TTTGCAGGGT GAGTGCAAGC TGAGTGATTG TAGCTTTAAG
    AGGGCATCTC CAGACACACC CTGTGTAGGC CTTCCACTGG AAAACCAGGT AGAACAGGCT
    TCTAGGTCAT GCCCAGCCCC TTCCCACCTT ACTTCAGAAT TGCTGAAGCT CAACAGCCAA
    GAGGAAGTGC CCAGGCCGGC TGAATGCAAG CCAGGGCACA GCCCAGAAAG CAGACTTGAG
    GAGCCCAAAG GTGTCCACCC TGATGATGTT ATCAGTAAAC AACAGTGCCT GCTAAACATC
    TCCGGAGAGG CAGGAAGCCA GACGGAGAAC TACCCATCAA TGAGTGCTCT CCAGGAGGAG
    AGAAAGCCGC CTGATAGGGC CTCCCTCTTC CCACAACACT GCTGCCATGG TGAGGTACCT
    GTGTGCAACA GAATGCTTGC AGGTTCCTGG GAAGAAGAGA GCGATAGCGC CTGTGCCCCA
    AGGACAGCCC TGAAATCAAG TGTCATTGGC TCTTTAGAAG CAGCAGAAGA GGGAGTGGTT
    CTAGACCTCC AACGCAATGG GAAGGCCTTG CAAAGTCTTT TGGACTTCCC AACATTGGAA
    GTATCTCACC GCAACCCTGG GAGTGAGTGT GCACAGGGAC CTGGGGATGA CCCGGAGCCT
    TCCCCTTCTG GCCAGCGGCA GGCTCCAGGA GCCCACGGGG CCTCCAGGCA CAGCTGCAAA
    TTCATTATGA TAGCAGTAAA GCAGGAAGGA AGGCAGACTT CTACAAGGAA GGCTCACTGT
    CTCATAGAAC CACAGTGTGG GCACCTTCCA GAGAAGGGGA CAAGCGCCAC GGCTTATGAG
    TCTTCCAGGG CCTCAGAAAT GCCCGTGTTT GGCAATGAAT GTGAGCTGCT GGGATGTCCA
    GGGGCAGAGG TGGATGGTGG GGCTCTTCAG CTGCTGGCCC AAGGCATAGA GCACGTGAGA
    GTGCCAGTCT CTAGGACTTC TTCGGATGAC ACTTTGAGCT CAGAGGAGAC ACTGGAGGGC
    GAGCTGCTTG GGGAAAGATT GGACTTAGGG TCTGAGTGTG TACACACCGA GCAGAACCAG
    GAGTTGCCCA GCTCAGATCG CAAACGAGGA AGGTGTGGCA CAGGCGATGC GGAGAATGCG
    GAAATCTGCG CCTGCGCGGG CGAGGTAGAG GGTCCCCTTT CAAGAGTCTT GGCTTCAAGC
    ACCACAACAC TGGAGTTCCA GGAACCATGC GGTGGGTTTC AGCAGAGTGT GCTGGAGTCA
    GAGCAGCACC CTGCCTGTCT TCAGATGACC CCTTTAAGAA CTGACATGCC CTGGTCCAGG
    GAGATGCCTG GCGGGGACAT ACCCTACAGC CAGGCCTTAA CCTCAGAGGA CTTGAACCCA
    GTTTGCCCTA GCCCCGAAGA AAGGGTCCCC CACAGTCCCT GCCAGGGGGA ACCACCTCTG
    CACCACGTGC ACCAGAGCGT ATCTCTGGAG ACCAAATCTG GTGTGATTCA AGTAGGGACA
    TCTGGGGACC TCAGCGGTGA AGGACCTTCC CCAGAAAGCT ACAACATCAA GAGACTGAAA
    ACAACAGAAA GCAAGATCAA AGCCAGGCTG GCCTTAGCCC ATAAGACCTT TACCAATTTT
    TTTGAAGCCA AAGTTATGGA GAAAACAAAT ACTGGCTCTG TCAAAGGCGA GAAAGAGAAG
    CGCAGGCGAG CCCGGGGCTC CTGGCGTGCG TTTCTGAGAA GCAAAGATGC GGAAGGCTCC
    AAACGACCTG CTTTAGTGAG TGGGGTTCCA GAACCAGGCT TTCCCCAACT CCTCTGCTCT
    TCCCCACCAG CCACCAGAGG CCACTGTGAG AAGTGGACGA TTGACACCGA CAGCCATGTA
    TTTGGGGAGC ACTGGACACC CCTAAACTCC CCTGCAACTC TGTCCTCCAG CCATCTGGCT
    TCTCCGGAAC ACCGGAGGAG AAGTGAACCG ACCATAAGAC GCACTGCTGC CCAGGAAAGC
    AGTACGCACC TCTCTTCTGG AGTCTTCCCA AAGTCTTCCT GGCTGACCTC TGCCACCAGC
    CCCGGAGCAG CGCAGATTGA CATCAGTTTT GCCCTCCCCT GCAGCTCTGC CTGCCATTTG
    ACCTATGAGA ACCAAGGCAT GCCCTGCAGA CCCACAAGCC CCAAACCCAG GAGTCCCATG
    GCTGGGGCGC AGAGGATGGA TCTCTGCCTT AGAGGCAGGA CTAGTGCTGT CTCCATGGTC
    TCTCTCAGAA GTGACAAGGA CGCCAACAGA AGTTCAGAAG TCCCCGAACG GCCCAAGGCA
    TCCAAGGCCA GGGCGAGCCT CTTACTCTCT CTGCAAACAC TAAACCAGAA CGACCAGAAG
    GAAGACAAGA GCCAGTGCCA GTGTTACCAT GGCCTGGGCA CAGTGCCCTT CCTTAGGGAC
    CATCCTGGGA ATGAGTGCTG GAGGAAGACA ACCATCACCT CTCCAGAGTC TCCGCATCTT
    CCTAGAAGAA GCCATCTGCT CTCCCAGAGT GCCCCAATGG GACTGGACCA CACAGGCTGG
    CCAGAGCCCA CGTCTGACAC TGGGATGCCT GACGGAACTC TGGACACGGT GTGCGCCGAG
    GAGACAGGCA GTGAGGAGGA CCTGTATGAA GAGCTTCACA ACTCTGGCCA CCACTACAGC
    CACCCTAGAG GAGGTGGAGA GCAACTGGCC ATCAATGAGC TCATCAGTGA TGGCAGTGTG
    GTGTGTGCTG AAGCCCTGTG GGACCATGTC ACCATGGATG ACCAGGAGCT GGGCTTCAAA
    GCTGGGGATG TCATCGAAGT GATGGATGCC ACCAACAGGG AATGGTGGTG GGGCCGAGTG
    GCAGATGGCG AGGGGTGGTT CCCTGCCAGC TTTGTACGGC TGCGCGTGAA CCAGGATGAG
    CCCGCGGATG ACTATGAGGC CCCGCGGGCC GGGGCTAGAG AGGCCGATGA CAGCGGTCCA
    GAGGCTCAGA GCTGCAAGGA CCAGATGCGC ACAAATGTCA TCAATGAGAT CCTCAGCACT
    GAGCGAGACT ACATCAAGCA CCTTCGCGAC ATCTGCGAGG GCTATGTCCG CCAGTGCCGC
    AAACGGGAGG ATATGTTCAG TGAAGAGCAG CTGCGCACCA TCTTTGGGAA CATCGAGGAC
    ATCTACCGCT GCCAAAAGGC ATTTGTGAAG GCTTTGGAGC AGAAGTTCAA CACGGAGCGG
    CCACACCTGA GCGAGTTGGG GGCCTGCTTT CTGGAACACC AAGCAGACTT CCAGATCTAT
    TCTGAGTACT GCAACAACCA CCCCAATGCC TGCGTGGAGC TCTCCCGGCT CACGAAGCTC
    AGCAAGTACG TGTACTTCTT CGAGGCCTGC CGGCTGCTGC AAAGGATGAT TGACATCTCG
    CTGGATGGCT TTCTGCTGAC CCCGGTGCAG AAGATCTGCA AGTATCCCCT CCAGCTGGCG
    GAGCTGCTCA AGTACACACA CCCCCAGCAC AGGGACTTTA AGAATGTTGA AGCTGCCTTG
    CACGCCATGA AGAATGTGGC CCAGCTCATC AACGAACGGA AACGAAGACT TGAAAACATC
    GACAAGATTG CTCAGTGGCA GAGCTCCATA GAGGACTGGG AGGGGGAAGA CCTTCTGGTC
    AGGAGCTCGG AACTCATCCA CTCGGGGGAG CTGACCCGCG TGACACAGCC TCAAGCCAGG
    AGTCAGCAGA GAATGTTTTT TCTCTTTGAC CGCCAGCTCA TCTACTGTAA AAAGGATCTG
    CTTCGCCGGG ACGTGCTGTA CTACAAGGGC CGGCTGGACA TGGATGACCT GGAGGTGGTA
    GATGTGGAAG ATGGGAAGGA CCGAGACCTC CATGTGAGTG TCAAGAACGC CTTCCGTCTG
    TACTGTGGTA CCACGGGGGA CAGCCACCTA CTGTGTGCCA GGAAGCCAGA ACAGAAGCAG
    CGCTGGCTGA AGGCCTTTGC CAGAGAGAGG GAGCAGGTGC GGCTGGACCA GGAGACAGGC
    TTCTCCATCA CCGAGCTGCA GAGGAAGCAG GCCATGCTGA ACGCCAGCAA GCAGCAGGCC
    ACGGGGAAGC CTAAAGCTGT GGGAAGGCCA GGATACCTGA CCCGCCACAA GCATCCATCA
    TTGCCTGCCA GCCGGCCCCA GCAGCAAGTG TTGGTGCTAG CAGAGCCCAG GCGGAAGCCA
    TCTAACTTCT GGCACAGCAT CAGCAGACTG GCGCCTTTCC GCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109464.1
    CDS122..5389
    Translation

    Target ORF information:

    RefSeq Version XM_030253604.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253604.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCTCGGTG TGGTGCACTT CCTTCGCAGC TTCTTTAAGA CTCCAGAGCC AGGTGTGCCC 
    CCTCCAGGTG AGGAAGAGTG TGAAGACAAC CAGCTCCACC CAGGCCCTGC AGACTCGGAG
    GAACAGGAAA CAAACCCCGA GGACTTGGAG ACACTGTCCC ATAAGAGCGA AAGTCGATCC
    GGATCCGATA CAAAAACAGA TCCCTTTGAA AGTGCTTCTG AGGCGGAGTC CCTCCCGGGT
    GACCTCACTG GTGGAGTTGG CCTCTCCGAA AGAGAAGCAG GCTGGGGATG CCCTGAGGAC
    TCAACAGTTA GACTTCCCCA AGGGCGGCAT TTGGTCTGTG ACGAAGGGCT CCATACAGAG
    GAAGAGCCGG ATCTACCCTC AGGCTTAAAG GCAGGTTCTC AGCTAAGTAA ATTGCACATG
    TTGACTGCTG GAAGACGGGA TGAAGCATGC CCCTGCAGCC GTGTGGCTAG CTTTGTACAG
    GAGTCTGTGC CAGCATCTGT GTGCTTCTCG AAGCATGCCA GCAGCAACAG CAGTCCCCAC
    CTGCAACAGG CCAGGTCTAG GGGCTATCTG GATGCCCCTG TGGTCTGTCC TTGCTTTCGT
    CGACACTGTG AACTAGGTCA GAGCTGGCCA CAAATCCACA TCAGGACTGC AGCAGCAAAG
    ATACCTCCCA CAGGCTCACA GCACAATGAC TTCCCTCTGT GGATCAGGAC AAAGAAGGGG
    CCTGCTCCGA GGTCCACAGT GGGCACAGAC TTGTTCTGGG TTCATCCCTT CCTTGACATA
    GCCTGTCAAC CTCGATTGGG CACACCTTAT CTCTTAGACA CCAAGTGTCA CCAGAGTACC
    CCAGAAAATC TTGGTGCCAA TGCCAAGCCA CCTCAAGGGT ACCTCTGCTG GCAGGTAAAG
    ACTCTGGTCA GGGCCCATTC TATAGCTTCA TTTCCTTTAG GTTGTCCTAA AGAGCCTCTG
    AAGCAGAAAG GTACAAATTG GCCCTACTTG CGGGAAGGGA CAGAAATAGA ACCCGACCAC
    CCAATCCTTC ACAAAACCCA GTCTCCTGGG CCCACCCAGC TGTCTCGTTT ACTTCCTACT
    TCTCAAAGCA GGGCCCCTCC TTTGCAGGGT GAGTGCAAGC TGAGTGATTG TAGCTTTAAG
    AGGGCATCTC CAGACACACC CTGTGTAGGC CTTCCACTGG AAAACCAGGT AGAACAGGCT
    TCTAGGTCAT GCCCAGCCCC TTCCCACCTT ACTTCAGAAT TGCTGAAGCT CAACAGCCAA
    GAGGAAGTGC CCAGGCCGGC TGAATGCAAG CCAGGGCACA GCCCAGAAAG CAGACTTGAG
    GAGCCCAAAG GTGTCCACCC TGATGATGTT ATCAGTAAAC AACAGTGCCT GCTAAACATC
    TCCGGAGAGG CAGGAAGCCA GACGGAGAAC TACCCATCAA TGAGTGCTCT CCAGGAGGAG
    AGAAAGCCGC CTGATAGGGC CTCCCTCTTC CCACAACACT GCTGCCATGG TGAGGTACCT
    GTGTGCAACA GAATGCTTGC AGGTTCCTGG GAAGAAGAGA GCGATAGCGC CTGTGCCCCA
    AGGACAGCCC TGAAATCAAG TGTCATTGGC TCTTTAGAAG CAGCAGAAGA GGGAGTGGTT
    CTAGACCTCC AACGCAATGG GAAGGCCTTG CAAAGTCTTT TGGACTTCCC AACATTGGAA
    GTATCTCACC GCAACCCTGG GAGTGAGTGT GCACAGGGAC CTGGGGATGA CCCGGAGCCT
    TCCCCTTCTG GCCAGCGGCA GGCTCCAGGA GCCCACGGGG CCTCCAGGCA CAGCTGCAAA
    TTCATTATGA TAGCAGTAAA GCAGGAAGGA AGGCAGACTT CTACAAGGAA GGCTCACTGT
    CTCATAGAAC CACAGTGTGG GCACCTTCCA GAGAAGGGGA CAAGCGCCAC GGCTTATGAG
    TCTTCCAGGG CCTCAGAAAT GCCCGTGTTT GGCAATGAAT GTGAGCTGCT GGGATGTCCA
    GGGGCAGAGG TGGATGGTGG GGCTCTTCAG CTGCTGGCCC AAGGCATAGA GCACGTGAGA
    GTGCCAGTCT CTAGGACTTC TTCGGATGAC ACTTTGAGCT CAGAGGAGAC ACTGGAGGGC
    GAGCTGCTTG GGGAAAGATT GGACTTAGGG TCTGAGTGTG TACACACCGA GCAGAACCAG
    GAGTTGCCCA GCTCAGATCG CAAACGAGGA AGGTGTGGCA CAGGCGATGC GGAGAATGCG
    GAAATCTGCG CCTGCGCGGG CGAGGTAGAG GGTCCCCTTT CAAGAGTCTT GGCTTCAAGC
    ACCACAACAC TGGAGTTCCA GGAACCATGC GGTGGGTTTC AGCAGAGTGT GCTGGAGTCA
    GAGCAGCACC CTGCCTGTCT TCAGATGACC CCTTTAAGAA CTGACATGCC CTGGTCCAGG
    GAGATGCCTG GCGGGGACAT ACCCTACAGC CAGGCCTTAA CCTCAGAGGA CTTGAACCCA
    GTTTGCCCTA GCCCCGAAGA AAGGGTCCCC CACAGTCCCT GCCAGGGGGA ACCACCTCTG
    CACCACGTGC ACCAGAGCGT ATCTCTGGAG ACCAAATCTG GTGTGATTCA AGTAGGGACA
    TCTGGGGACC TCAGCGGTGA AGGACCTTCC CCAGAAAGCT ACAACATCAA GAGACTGAAA
    ACAACAGAAA GCAAGATCAA AGCCAGGCTG GCCTTAGCCC ATAAGACCTT TACCAATTTT
    TTTGAAGCCA AAGTTATGGA GAAAACAAAT ACTGGCTCTG TCAAAGGCGA GAAAGAGAAG
    CGCAGGCGAG CCCGGGGCTC CTGGCGTGCG TTTCTGAGAA GCAAAGATGC GGAAGGCTCC
    AAACGACCTG CTTTAGTGAG TGGGGTTCCA GAACCAGGCT TTCCCCAACT CCTCTGCTCT
    TCCCCACCAG CCACCAGAGG CCACTGTGAG AAGTGGACGA TTGACACCGA CAGCCATGTA
    TTTGGGGAGC ACTGGACACC CCTAAACTCC CCTGCAACTC TGTCCTCCAG CCATCTGGCT
    TCTCCGGAAC ACCGGAGGAG AAGTGAACCG ACCATAAGAC GCACTGCTGC CCAGGAAAGC
    AGTACGCACC TCTCTTCTGG AGTCTTCCCA AAGTCTTCCT GGCTGACCTC TGCCACCAGC
    CCCGGAGCAG CGCAGATTGA CATCAGTTTT GCCCTCCCCT GCAGCTCTGC CTGCCATTTG
    ACCTATGAGA ACCAAGGCAT GCCCTGCAGA CCCACAAGCC CCAAACCCAG GAGTCCCATG
    GCTGGGGCGC AGAGGATGGA TCTCTGCCTT AGAGGCAGGA CTAGTGCTGT CTCCATGGTC
    TCTCTCAGAA GTGACAAGGA CGCCAACAGA AGTTCAGAAG TCCCCGAACG GCCCAAGGCA
    TCCAAGGCCA GGGCGAGCCT CTTACTCTCT CTGCAAACAC TAAACCAGAA CGACCAGAAG
    GAAGACAAGA GCCAGTGCCA GTGTTACCAT GGCCTGGGCA CAGTGCCCTT CCTTAGGGAC
    CATCCTGGGA ATGAGTGCTG GAGGAAGACA ACCATCACCT CTCCAGAGTC TCCGCATCTT
    CCTAGAAGAA GCCATCTGCT CTCCCAGAGT GCCCCAATGG GACTGGACCA CACAGGCTGG
    CCAGAGCCCA CGTCTGACAC TGGGATGCCT GACGGAACTC TGGACACGGT GTGCGCCGAG
    GAGACAGGCA GTGAGGAGGA CCTGTATGAA GAGCTTCACA ACTCTGGCCA CCACTACAGC
    CACCCTAGAG GAGGTGGAGA GCAACTGGCC ATCAATGAGC TCATCAGTGA TGGCAGTGTG
    GTGTGTGCTG AAGCCCTGTG GGACCATGTC ACCATGGATG ACCAGGAGCT GGGCTTCAAA
    GCTGGGGATG TCATCGAAGT GATGGATGCC ACCAACAGGG AATGGTGGTG GGGCCGAGTG
    GCAGATGGCG AGGGGTGGTT CCCTGCCAGC TTTGTACGGC TGCGCGTGAA CCAGGATGAG
    CCCGCGGATG ACTATGAGGC CCCGCGGGCC GGGGCTAGAG AGGCCGATGA CAGCGGTCCA
    GAGGCTCAGA GCTGCAAGGA CCAGATGCGC ACAAATGTCA TCAATGAGAT CCTCAGCACT
    GAGCGAGACT ACATCAAGCA CCTTCGCGAC ATCTGCGAGG GCTATGTCCG CCAGTGCCGC
    AAACGGGAGG ATATGTTCAG TGAAGAGCAG CTGCGCACCA TCTTTGGGAA CATCGAGGAC
    ATCTACCGCT GCCAAAAGGC ATTTGTGAAG GCTTTGGAGC AGAAGTTCAA CACGGAGCGG
    CCACACCTGA GCGAGTTGGG GGCCTGCTTT CTGGAACACC AAGCAGACTT CCAGATCTAT
    TCTGAGTACT GCAACAACCA CCCCAATGCC TGCGTGGAGC TCTCCCGGCT CACGAAGCTC
    AGCAAGTACG TGTACTTCTT CGAGGCCTGC CGGCTGCTGC AAAGGATGAT TGACATCTCG
    CTGGATGGCT TTCTGCTGAC CCCGGTGCAG AAGATCTGCA AGTATCCCCT CCAGCTGGCG
    GAGCTGCTCA AGTACACACA CCCCCAGCAC AGGGACTTTA AGAATGTTGA AGCTGCCTTG
    CACGCCATGA AGAATGTGGC CCAGCTCATC AACGAACGGA AACGAAGACT TGAAAACATC
    GACAAGATTG CTCAGTGGCA GAGCTCCATA GAGGACTGGG AGGGGGAAGA CCTTCTGGTC
    AGGAGCTCGG AACTCATCCA CTCGGGGGAG CTGACCCGCG TGACACAGCC TCAAGCCAGG
    AGTCAGCAGA GAATGTTTTT TCTCTTTGAC CGCCAGCTCA TCTACTGTAA AAAGGATCTG
    CTTCGCCGGG ACGTGCTGTA CTACAAGGGC CGGCTGGACA TGGATGACCT GGAGGTGGTA
    GATGTGGAAG ATGGGAAGGA CCGAGACCTC CATGTGAGTG TCAAGAACGC CTTCCGTCTG
    TACTGTGGTA CCACGGGGGA CAGCCACCTA CTGTGTGCCA GGAAGCCAGA ACAGAAGCAG
    CGCTGGCTGA AGGCCTTTGC CAGAGAGAGG GAGCAGGTGC GGCTGGACCA GGAGACAGGC
    TTCTCCATCA CCGAGCTGCA GAGGAAGCAG GCCATGCTGA ACGCCAGCAA GCAGCAGGCC
    ACGGGGAAGC CTAAAGCTGT GGGAAGGCCA GGATACCTGA CCCGCCACAA GCATCCATCA
    TTGCCTGCCA GCCGGCCCCA GCAGCAAGTG TTGGTGCTAG CAGAGCCCAG GCGGAAGCCA
    TCTAACTTCT GGCACAGCAT CAGCAGACTG GCGCCTTTCC GCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85138
    Clone ID Related Accession (Same CDS sequence) XM_030253606.1
    Accession Version XM_030253606.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTTGACTG CTGGAAGACG GGATGAAGCA TGCCCCTGCA GCCGTGTGGC TAGCTTTGTA 
    CAGGAGTCTG TGCCAGCATC TGTGTGCTTC TCGAAGCATG CCAGCAGCAA CAGCAGTCCC
    CACCTGCAAC AGGCCAGGTC TAGGGGCTAT CTGGATGCCC CTGTGGTCTG TCCTTGCTTT
    CGTCGACACT GTGAACTAGG TCAGAGCTGG CCACAAATCC ACATCAGGAC TGCAGCAGCA
    AAGATACCTC CCACAGGCTC ACAGCACAAT GACTTCCCTC TGTGGATCAG GACAAAGAAG
    GGGCCTGCTC CGAGGTCCAC AGTGGGCACA GACTTGTTCT GGGTTCATCC CTTCCTTGAC
    ATAGCCTGTC AACCTCGATT GGGCACACCT TATCTCTTAG ACACCAAGTG TCACCAGAGT
    ACCCCAGAAA ATCTTGGTGC CAATGCCAAG CCACCTCAAG GGTACCTCTG CTGGCAGGTA
    AAGACTCTGG TCAGGGCCCA TTCTATAGCT TCATTTCCTT TAGGTTGTCC TAAAGAGCCT
    CTGAAGCAGA AAGGTACAAA TTGGCCCTAC TTGCGGGAAG GGACAGAAAT AGAACCCGAC
    CACCCAATCC TTCACAAAAC CCAGTCTCCT GGGCCCACCC AGCTGTCTCG TTTACTTCCT
    ACTTCTCAAA GCAGGGCCCC TCCTTTGCAG GGTGAGTGCA AGCTGAGTGA TTGTAGCTTT
    AAGAGGGCAT CTCCAGACAC ACCCTGTGTA GGCCTTCCAC TGGAAAACCA GGTAGAACAG
    GCTTCTAGGT CATGCCCAGC CCCTTCCCAC CTTACTTCAG AATTGCTGAA GCTCAACAGC
    CAAGAGGAAG TGCCCAGGCC GGCTGAATGC AAGCCAGGGC ACAGCCCAGA AAGCAGACTT
    GAGGAGCCCA AAGGTGTCCA CCCTGATGAT GTTATCAGTA AACAACAGTG CCTGCTAAAC
    ATCTCCGGAG AGGCAGGAAG CCAGACGGAG AACTACCCAT CAATGAGTGC TCTCCAGGAG
    GAGAGAAAGC CGCCTGATAG GGCCTCCCTC TTCCCACAAC ACTGCTGCCA TGGTGAGGTA
    CCTGTGTGCA ACAGAATGCT TGCAGGTTCC TGGGAAGAAG AGAGCGATAG CGCCTGTGCC
    CCAAGGACAG CCCTGAAATC AAGTGTCATT GGCTCTTTAG AAGCAGCAGA AGAGGGAGTG
    GTTCTAGACC TCCAACGCAA TGGGAAGGCC TTGCAAAGTC TTTTGGACTT CCCAACATTG
    GAAGTATCTC ACCGCAACCC TGGGAGTGAG TGTGCACAGG GACCTGGGGA TGACCCGGAG
    CCTTCCCCTT CTGGCCAGCG GCAGGCTCCA GGAGCCCACG GGGCCTCCAG GCACAGCTGC
    AAATTCATTA TGATAGCAGT AAAGCAGGAA GGAAGGCAGA CTTCTACAAG GAAGGCTCAC
    TGTCTCATAG AACCACAGTG TGGGCACCTT CCAGAGAAGG GGACAAGCGC CACGGCTTAT
    GAGTCTTCCA GGGCCTCAGA AATGCCCGTG TTTGGCAATG AATGTGAGCT GCTGGGATGT
    CCAGGGGCAG AGGTGGATGG TGGGGCTCTT CAGCTGCTGG CCCAAGGCAT AGAGCACGTG
    AGAGTGCCAG TCTCTAGGAC TTCTTCGGAT GACACTTTGA GCTCAGAGGA GACACTGGAG
    GGCGAGCTGC TTGGGGAAAG ATTGGACTTA GGGTCTGAGT GTGTACACAC CGAGCAGAAC
    CAGGAGTTGC CCAGCTCAGA TCGCAAACGA GGAAGGTGTG GCACAGGCGA TGCGGAGAAT
    GCGGAAATCT GCGCCTGCGC GGGCGAGGTA GAGGGTCCCC TTTCAAGAGT CTTGGCTTCA
    AGCACCACAA CACTGGAGTT CCAGGAACCA TGCGGTGGGT TTCAGCAGAG TGTGCTGGAG
    TCAGAGCAGC ACCCTGCCTG TCTTCAGATG ACCCCTTTAA GAACTGACAT GCCCTGGTCC
    AGGGAGATGC CTGGCGGGGA CATACCCTAC AGCCAGGCCT TAACCTCAGA GGACTTGAAC
    CCAGTTTGCC CTAGCCCCGA AGAAAGGGTC CCCCACAGTC CCTGCCAGGG GGAACCACCT
    CTGCACCACG TGCACCAGAG CGTATCTCTG GAGACCAAAT CTGGTGTGAT TCAAGTAGGG
    ACATCTGGGG ACCTCAGCGG TGAAGGACCT TCCCCAGAAA GCTACAACAT CAAGAGACTG
    AAAACAACAG AAAGCAAGAT CAAAGCCAGG CTGGCCTTAG CCCATAAGAC CTTTACCAAT
    TTTTTTGAAG CCAAAGTTAT GGAGAAAACA AATACTGGCT CTGTCAAAGG CGAGAAAGAG
    AAGCGCAGGC GAGCCCGGGG CTCCTGGCGT GCGTTTCTGA GAAGCAAAGA TGCGGAAGGC
    TCCAAACGAC CTGCTTTAGT GAGTGGGGTT CCAGAACCAG GCTTTCCCCA ACTCCTCTGC
    TCTTCCCCAC CAGCCACCAG AGGCCACTGT GAGAAGTGGA CGATTGACAC CGACAGCCAT
    GTATTTGGGG AGCACTGGAC ACCCCTAAAC TCCCCTGCAA CTCTGTCCTC CAGCCATCTG
    GCTTCTCCGG AACACCGGAG GAGAAGTGAA CCGACCATAA GACGCACTGC TGCCCAGGAA
    AGCAGTACGC ACCTCTCTTC TGGAGTCTTC CCAAAGTCTT CCTGGCTGAC CTCTGCCACC
    AGCCCCGGAG CAGCGCAGAT TGACATCAGT TTTGCCCTCC CCTGCAGCTC TGCCTGCCAT
    TTGACCTATG AGAACCAAGG CATGCCCTGC AGACCCACAA GCCCCAAACC CAGGAGTCCC
    ATGGCTGGGG CGCAGAGGAT GGATCTCTGC CTTAGAGGCA GGACTAGTGC TGTCTCCATG
    GTCTCTCTCA GAAGTGACAA GGACGCCAAC AGAAGTTCAG AAGTCCCCGA ACGGCCCAAG
    GCATCCAAGG CCAGGGCGAG CCTCTTACTC TCTCTGCAAA CACTAAACCA GAACGACCAG
    AAGGAAGACA AGAGCCAGTG CCAGTGTTAC CATGGCCTGG GCACAGTGCC CTTCCTTAGG
    GACCATCCTG GGAATGAGAG CTACATCGCC CAGGAAAGCC CGCCAGACAA GAAGCGCAAT
    TGTTCCCATG ATCAGAAAAC TTACACAGAG CCTCCTCACA GGCCGGTATC TGCCACTGAA
    GTGACACGGA CCCTCCCCTC CATGTCCGCA GAGCAAGTCC CCAGAGAGGT CCCTTTACAG
    TTCAGGAGGG CCCCTCAACA CTCACACTTC AGCTTCGATG ACATATGGCT CGAAAAGGCT
    CAGAGGAGGA AGCTGAAGAA ACAGATGCTG CTTAGACGAC AGATGTGCGC AGAGTTAGTG
    CCTGAGGACC CAGTTCAGTG CTGGAGGAAG ACAACCATCA CCTCTCCAGA GTCTCCGCAT
    CTTCCTAGAA GAAGCCATCT GCTCTCCCAG AGTGCCCCAA TGGGACTGGA CCACACAGGC
    TGGCCAGAGC CCACGTCTGA CACTGGGATG CCTGACGGAA CTCTGGACAC GGTGTGCGCC
    GAGGAGACAG GCAGTGAGGA GGACCTGTAT GAAGAGCTTC ACAACTCTGG CCACCACTAC
    AGCCACCCTA GAGGAGGTGG AGAGCAACTG GCCATCAATG AGCTCATCAG TGATGGCAGT
    GTGGTGTGTG CTGAAGCCCT GTGGGACCAT GTCACCATGG ATGACCAGGA GCTGGGCTTC
    AAAGCTGGGG ATGTCATCGA AGTGATGGAT GCCACCAACA GGGAATGGTG GTGGGGCCGA
    GTGGCAGATG GCGAGGGGTG GTTCCCTGCC AGCTTTGTAC GGCTGCGCGT GAACCAGGAT
    GAGCCCGCGG ATGACTATGA GGCCCCGCGG GCCGGGGCTA GAGAGGCCGA TGACAGCGGT
    CCAGAGGCTC AGAGCTGCAA GGACCAGATG CGCACAAATG TCATCAATGA GATCCTCAGC
    ACTGAGCGAG ACTACATCAA GCACCTTCGC GACATCTGCG AGGGCTATGT CCGCCAGTGC
    CGCAAACGGG AGGATATGTT CAGTGAAGAG CAGCTGCGCA CCATCTTTGG GAACATCGAG
    GACATCTACC GCTGCCAAAA GGCATTTGTG AAGGCTTTGG AGCAGAAGTT CAACACGGAG
    CGGCCACACC TGAGCGAGTT GGGGGCCTGC TTTCTGGAAC ACCAAGCAGA CTTCCAGATC
    TATTCTGAGT ACTGCAACAA CCACCCCAAT GCCTGCGTGG AGCTCTCCCG GCTCACGAAG
    CTCAGCAAGT ACGTGTACTT CTTCGAGGCC TGCCGGCTGC TGCAAAGGAT GATTGACATC
    TCGCTGGATG GCTTTCTGCT GACCCCGGTG CAGAAGATCT GCAAGTATCC CCTCCAGCTG
    GCGGAGCTGC TCAAGTACAC ACACCCCCAG CACAGGGACT TTAAGAATGT TGAAGCTGCC
    TTGCACGCCA TGAAGAATGT GGCCCAGCTC ATCAACGAAC GGAAACGAAG ACTTGAAAAC
    ATCGACAAGA TTGCTCAGTG GCAGAGCTCC ATAGAGGACT GGGAGGGGGA AGACCTTCTG
    GTCAGGAGCT CGGAACTCAT CCACTCGGGG GAGCTGACCC GCGTGACACA GCCTCAAGCC
    AGGAGTCAGC AGAGAATGTT TTTTCTCTTT GACCGCCAGC TCATCTACTG TAAAAAGGAT
    CTGCTTCGCC GGGACGTGCT GTACTACAAG GGCCGGCTGG ACATGGATGA CCTGGAGGTG
    GTAGATGTGG AAGATGGGAA GGACCGAGAC CTCCATGTGA GTGTCAAGAA CGCCTTCCGT
    CTGTACTGTG GTACCACGGG GGACAGCCAC CTACTGTGTG CCAGGAAGCC AGAACAGAAG
    CAGCGCTGGC TGAAGGCCTT TGCCAGAGAG AGGGAGCAGG TGCGGCTGGA CCAGGAGACA
    GGCTTCTCCA TCACCGAGCT GCAGAGGAAG CAGGCCATGC TGAACGCCAG CAAGCAGCAG
    GCCACGGGGA AGCCTAAAGC TGTGGGAAGG CCAGGATACC TGACCCGCCA CAAGCATCCA
    TCATTGCCTG CCAGCCGGCC CCAGCAGCAA GTGTTGGTGC TAGCAGAGCC CAGGCGGAAG
    CCATCTAACT TCTGGCACAG CATCAGCAGA CTGGCGCCTT TCCGCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109466.1
    CDS545..5695
    Translation

    Target ORF information:

    RefSeq Version XM_030253606.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253606.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTTGACTG CTGGAAGACG GGATGAAGCA TGCCCCTGCA GCCGTGTGGC TAGCTTTGTA 
    CAGGAGTCTG TGCCAGCATC TGTGTGCTTC TCGAAGCATG CCAGCAGCAA CAGCAGTCCC
    CACCTGCAAC AGGCCAGGTC TAGGGGCTAT CTGGATGCCC CTGTGGTCTG TCCTTGCTTT
    CGTCGACACT GTGAACTAGG TCAGAGCTGG CCACAAATCC ACATCAGGAC TGCAGCAGCA
    AAGATACCTC CCACAGGCTC ACAGCACAAT GACTTCCCTC TGTGGATCAG GACAAAGAAG
    GGGCCTGCTC CGAGGTCCAC AGTGGGCACA GACTTGTTCT GGGTTCATCC CTTCCTTGAC
    ATAGCCTGTC AACCTCGATT GGGCACACCT TATCTCTTAG ACACCAAGTG TCACCAGAGT
    ACCCCAGAAA ATCTTGGTGC CAATGCCAAG CCACCTCAAG GGTACCTCTG CTGGCAGGTA
    AAGACTCTGG TCAGGGCCCA TTCTATAGCT TCATTTCCTT TAGGTTGTCC TAAAGAGCCT
    CTGAAGCAGA AAGGTACAAA TTGGCCCTAC TTGCGGGAAG GGACAGAAAT AGAACCCGAC
    CACCCAATCC TTCACAAAAC CCAGTCTCCT GGGCCCACCC AGCTGTCTCG TTTACTTCCT
    ACTTCTCAAA GCAGGGCCCC TCCTTTGCAG GGTGAGTGCA AGCTGAGTGA TTGTAGCTTT
    AAGAGGGCAT CTCCAGACAC ACCCTGTGTA GGCCTTCCAC TGGAAAACCA GGTAGAACAG
    GCTTCTAGGT CATGCCCAGC CCCTTCCCAC CTTACTTCAG AATTGCTGAA GCTCAACAGC
    CAAGAGGAAG TGCCCAGGCC GGCTGAATGC AAGCCAGGGC ACAGCCCAGA AAGCAGACTT
    GAGGAGCCCA AAGGTGTCCA CCCTGATGAT GTTATCAGTA AACAACAGTG CCTGCTAAAC
    ATCTCCGGAG AGGCAGGAAG CCAGACGGAG AACTACCCAT CAATGAGTGC TCTCCAGGAG
    GAGAGAAAGC CGCCTGATAG GGCCTCCCTC TTCCCACAAC ACTGCTGCCA TGGTGAGGTA
    CCTGTGTGCA ACAGAATGCT TGCAGGTTCC TGGGAAGAAG AGAGCGATAG CGCCTGTGCC
    CCAAGGACAG CCCTGAAATC AAGTGTCATT GGCTCTTTAG AAGCAGCAGA AGAGGGAGTG
    GTTCTAGACC TCCAACGCAA TGGGAAGGCC TTGCAAAGTC TTTTGGACTT CCCAACATTG
    GAAGTATCTC ACCGCAACCC TGGGAGTGAG TGTGCACAGG GACCTGGGGA TGACCCGGAG
    CCTTCCCCTT CTGGCCAGCG GCAGGCTCCA GGAGCCCACG GGGCCTCCAG GCACAGCTGC
    AAATTCATTA TGATAGCAGT AAAGCAGGAA GGAAGGCAGA CTTCTACAAG GAAGGCTCAC
    TGTCTCATAG AACCACAGTG TGGGCACCTT CCAGAGAAGG GGACAAGCGC CACGGCTTAT
    GAGTCTTCCA GGGCCTCAGA AATGCCCGTG TTTGGCAATG AATGTGAGCT GCTGGGATGT
    CCAGGGGCAG AGGTGGATGG TGGGGCTCTT CAGCTGCTGG CCCAAGGCAT AGAGCACGTG
    AGAGTGCCAG TCTCTAGGAC TTCTTCGGAT GACACTTTGA GCTCAGAGGA GACACTGGAG
    GGCGAGCTGC TTGGGGAAAG ATTGGACTTA GGGTCTGAGT GTGTACACAC CGAGCAGAAC
    CAGGAGTTGC CCAGCTCAGA TCGCAAACGA GGAAGGTGTG GCACAGGCGA TGCGGAGAAT
    GCGGAAATCT GCGCCTGCGC GGGCGAGGTA GAGGGTCCCC TTTCAAGAGT CTTGGCTTCA
    AGCACCACAA CACTGGAGTT CCAGGAACCA TGCGGTGGGT TTCAGCAGAG TGTGCTGGAG
    TCAGAGCAGC ACCCTGCCTG TCTTCAGATG ACCCCTTTAA GAACTGACAT GCCCTGGTCC
    AGGGAGATGC CTGGCGGGGA CATACCCTAC AGCCAGGCCT TAACCTCAGA GGACTTGAAC
    CCAGTTTGCC CTAGCCCCGA AGAAAGGGTC CCCCACAGTC CCTGCCAGGG GGAACCACCT
    CTGCACCACG TGCACCAGAG CGTATCTCTG GAGACCAAAT CTGGTGTGAT TCAAGTAGGG
    ACATCTGGGG ACCTCAGCGG TGAAGGACCT TCCCCAGAAA GCTACAACAT CAAGAGACTG
    AAAACAACAG AAAGCAAGAT CAAAGCCAGG CTGGCCTTAG CCCATAAGAC CTTTACCAAT
    TTTTTTGAAG CCAAAGTTAT GGAGAAAACA AATACTGGCT CTGTCAAAGG CGAGAAAGAG
    AAGCGCAGGC GAGCCCGGGG CTCCTGGCGT GCGTTTCTGA GAAGCAAAGA TGCGGAAGGC
    TCCAAACGAC CTGCTTTAGT GAGTGGGGTT CCAGAACCAG GCTTTCCCCA ACTCCTCTGC
    TCTTCCCCAC CAGCCACCAG AGGCCACTGT GAGAAGTGGA CGATTGACAC CGACAGCCAT
    GTATTTGGGG AGCACTGGAC ACCCCTAAAC TCCCCTGCAA CTCTGTCCTC CAGCCATCTG
    GCTTCTCCGG AACACCGGAG GAGAAGTGAA CCGACCATAA GACGCACTGC TGCCCAGGAA
    AGCAGTACGC ACCTCTCTTC TGGAGTCTTC CCAAAGTCTT CCTGGCTGAC CTCTGCCACC
    AGCCCCGGAG CAGCGCAGAT TGACATCAGT TTTGCCCTCC CCTGCAGCTC TGCCTGCCAT
    TTGACCTATG AGAACCAAGG CATGCCCTGC AGACCCACAA GCCCCAAACC CAGGAGTCCC
    ATGGCTGGGG CGCAGAGGAT GGATCTCTGC CTTAGAGGCA GGACTAGTGC TGTCTCCATG
    GTCTCTCTCA GAAGTGACAA GGACGCCAAC AGAAGTTCAG AAGTCCCCGA ACGGCCCAAG
    GCATCCAAGG CCAGGGCGAG CCTCTTACTC TCTCTGCAAA CACTAAACCA GAACGACCAG
    AAGGAAGACA AGAGCCAGTG CCAGTGTTAC CATGGCCTGG GCACAGTGCC CTTCCTTAGG
    GACCATCCTG GGAATGAGAG CTACATCGCC CAGGAAAGCC CGCCAGACAA GAAGCGCAAT
    TGTTCCCATG ATCAGAAAAC TTACACAGAG CCTCCTCACA GGCCGGTATC TGCCACTGAA
    GTGACACGGA CCCTCCCCTC CATGTCCGCA GAGCAAGTCC CCAGAGAGGT CCCTTTACAG
    TTCAGGAGGG CCCCTCAACA CTCACACTTC AGCTTCGATG ACATATGGCT CGAAAAGGCT
    CAGAGGAGGA AGCTGAAGAA ACAGATGCTG CTTAGACGAC AGATGTGCGC AGAGTTAGTG
    CCTGAGGACC CAGTTCAGTG CTGGAGGAAG ACAACCATCA CCTCTCCAGA GTCTCCGCAT
    CTTCCTAGAA GAAGCCATCT GCTCTCCCAG AGTGCCCCAA TGGGACTGGA CCACACAGGC
    TGGCCAGAGC CCACGTCTGA CACTGGGATG CCTGACGGAA CTCTGGACAC GGTGTGCGCC
    GAGGAGACAG GCAGTGAGGA GGACCTGTAT GAAGAGCTTC ACAACTCTGG CCACCACTAC
    AGCCACCCTA GAGGAGGTGG AGAGCAACTG GCCATCAATG AGCTCATCAG TGATGGCAGT
    GTGGTGTGTG CTGAAGCCCT GTGGGACCAT GTCACCATGG ATGACCAGGA GCTGGGCTTC
    AAAGCTGGGG ATGTCATCGA AGTGATGGAT GCCACCAACA GGGAATGGTG GTGGGGCCGA
    GTGGCAGATG GCGAGGGGTG GTTCCCTGCC AGCTTTGTAC GGCTGCGCGT GAACCAGGAT
    GAGCCCGCGG ATGACTATGA GGCCCCGCGG GCCGGGGCTA GAGAGGCCGA TGACAGCGGT
    CCAGAGGCTC AGAGCTGCAA GGACCAGATG CGCACAAATG TCATCAATGA GATCCTCAGC
    ACTGAGCGAG ACTACATCAA GCACCTTCGC GACATCTGCG AGGGCTATGT CCGCCAGTGC
    CGCAAACGGG AGGATATGTT CAGTGAAGAG CAGCTGCGCA CCATCTTTGG GAACATCGAG
    GACATCTACC GCTGCCAAAA GGCATTTGTG AAGGCTTTGG AGCAGAAGTT CAACACGGAG
    CGGCCACACC TGAGCGAGTT GGGGGCCTGC TTTCTGGAAC ACCAAGCAGA CTTCCAGATC
    TATTCTGAGT ACTGCAACAA CCACCCCAAT GCCTGCGTGG AGCTCTCCCG GCTCACGAAG
    CTCAGCAAGT ACGTGTACTT CTTCGAGGCC TGCCGGCTGC TGCAAAGGAT GATTGACATC
    TCGCTGGATG GCTTTCTGCT GACCCCGGTG CAGAAGATCT GCAAGTATCC CCTCCAGCTG
    GCGGAGCTGC TCAAGTACAC ACACCCCCAG CACAGGGACT TTAAGAATGT TGAAGCTGCC
    TTGCACGCCA TGAAGAATGT GGCCCAGCTC ATCAACGAAC GGAAACGAAG ACTTGAAAAC
    ATCGACAAGA TTGCTCAGTG GCAGAGCTCC ATAGAGGACT GGGAGGGGGA AGACCTTCTG
    GTCAGGAGCT CGGAACTCAT CCACTCGGGG GAGCTGACCC GCGTGACACA GCCTCAAGCC
    AGGAGTCAGC AGAGAATGTT TTTTCTCTTT GACCGCCAGC TCATCTACTG TAAAAAGGAT
    CTGCTTCGCC GGGACGTGCT GTACTACAAG GGCCGGCTGG ACATGGATGA CCTGGAGGTG
    GTAGATGTGG AAGATGGGAA GGACCGAGAC CTCCATGTGA GTGTCAAGAA CGCCTTCCGT
    CTGTACTGTG GTACCACGGG GGACAGCCAC CTACTGTGTG CCAGGAAGCC AGAACAGAAG
    CAGCGCTGGC TGAAGGCCTT TGCCAGAGAG AGGGAGCAGG TGCGGCTGGA CCAGGAGACA
    GGCTTCTCCA TCACCGAGCT GCAGAGGAAG CAGGCCATGC TGAACGCCAG CAAGCAGCAG
    GCCACGGGGA AGCCTAAAGC TGTGGGAAGG CCAGGATACC TGACCCGCCA CAAGCATCCA
    TCATTGCCTG CCAGCCGGCC CCAGCAGCAA GTGTTGGTGC TAGCAGAGCC CAGGCGGAAG
    CCATCTAACT TCTGGCACAG CATCAGCAGA CTGGCGCCTT TCCGCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.