arhgef4 cDNA ORF clone, Danio rerio(zebrafish)

The following arhgef4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa06855 XM_682312.8
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa150441 XM_021467029.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa150442 XM_021467031.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42946 XM_009298437.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42947 XM_009298440.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42948 XM_009298441.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42949 XM_009298442.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42950 XM_009298443.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa42948 XM_009298441.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42947 XM_009298440.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa06855 XM_682312.7 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa42949 XM_009298442.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42946 XM_009298437.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42950 XM_009298443.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa06855
    Clone ID Related Accession (Same CDS sequence) XM_682312.7 , XM_682312.8
    Accession Version XM_682312.7 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_682312.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCGCACCC AGCACAGTGC CGGTGAGGTG GGCGTCCCGC TGGCAGGAGG AGGCTGCAGT 
    GGTGGCAGCT CAGAGCAGTC GCCTGGCTCC GACTTGGACT GTAGCCCCGC TCTGGGACTG
    GCCGGAGGAG TTCGAGCGAT AAGAGGGGTC ATGTCCCATT ACTGGAGCCT AGAGAGCCTG
    CACTCCACCA ACGCATTGGT GATAACTGAT GGGCCACAGG ATAAGTCTGG TCTTGCAGAT
    GAGGTGGGCA GTGAAGATGA TCTGTACAAT GAATTCCGCA GCTCCTCCAA CCGATTCGGA
    CACCCAGGAG GAGGAGGAGA ACAGCTGGCC ATAAATGAGC TGATCAGTGA TGGCAGTGTG
    TGCGCGGAGG CTCTATGGGA CCACGTCACC ATGGATGACC AGGAGCTGGG CTTTAAAGCT
    GGAGATGTCA TTGAAGTAGT GGACGCTACG AACAAGGAGT GGTGGTGGGG CAGGGTGCTG
    GACAGCGAGG GCTGGTTCCC CGCCAGCTTT GTTCGGTTAC GGGTGAACCA AGATGAGCCC
    ATGGAGGAAT ACCTAGCCCA TCTGGACGGG GCCTCAGAGG GGTGTGGGGC CAGCATGGGG
    GGACCCTTGG GACCTGGACT GCCATGCAAG GAGCAGATGA GAGCCAACGT CATCAATGAG
    ATCATGAGCA CAGAGAGAGA TTACATTAAA CACCTGAAGG ACATATGTGA GGGCTATATA
    AAACAATGTC GGAAGAGGAC AGATATGTTT ACAGAAGAGC AACTGCGCAC TATCTTTGGC
    AACATCGACG AGCTCTACCG ATTCCAGAAG AAGTTCCTCA AAGCCTTGGA GAAAAAATTC
    AACAAAGAGC ATCCTCACCT CAGCGAGATC GGCTCCTGCT TCTTAGAGCA GCAAACAAAC
    TTCCAGATCT ATTCTGAGTA TTGCAACAAC CACCCCAATG CCTGCGTGCA GCTCTCCAAA
    CTGATGAAGA TCAAGAAGTA TGTGTTCTTC TTTGAGGCTT GTCGCCTGCT CCAGAAGATG
    ATTGACATTT CGTTGGATGG ATTCCTACTT ACTCCTGTTC AGAAGATCTG CAAGTATCCT
    CTGCAGCTCG CTGAACTGCT CAAGTACACC AACCCACAGC ACAGAGATTA TAAGGATGTG
    GAAGCTGCCT TAAACGCTAT GAAGAATGTG GCCAGACTGA TCAATGAGAG AAAGAGGCGT
    CTAGAAAATA TTGACAAAAT CGCTCAGTGG CAGAGCTCCA TAGAAGACTG GGAGGGTGAA
    GACGTCTTGA GCAGGAGCTC TGATCTGATC TTTTCTGGAG ACCTGACCAA GATCTCCCAG
    CCACAGGCTA AAGGCCAGCA GAGAATGTTC TTCCTCTTTG ACCACCAACT GGTTTTCTGT
    AAGAAGGATC TGCTTCGGAG GGATATCTTG TACTACAAGG GTCGGTTAGA CATGGATGAG
    ATGGAGGTGG TGGATGTGGA GGATGGGAAG GATAAGGACT TTAATGTGAG TGTGAAGAAT
    GCCTTGAAAT TGTGTTCTCC TGGAGGAGAG GAGGTTCATC TCCTGTGTGC CAAGAAGCCC
    GAACAAAAGC AGCGCTGGCT GAGAGCCTTC ACAGATGAGC GGGAGCAGGT CCAGCATGAC
    CTCGAGACAG GTTTTACGAT TACAGAGGTC CAGAAGAAGC AGGCAATGTT GAATGCAACT
    AAGAGTCATC CAACAGGGAA GCCTAAAGCA TTAGCTTTAC AGCACCTTTT GGCACTCAGA
    GCTCCACTCC TTCACCCCAA AACCAAGTGC CTGCGTTTGT CCGAGCCTCC AGGACCTCTG
    GTTCCCAGGG CCACCCTAAC TTCACCTCTC CTCCCTGAAG GGCCTCAGCG CAGGCAGGGG
    CGGGGGGTTA TGGTGCCCCC TGGGGTCCTG CGCAGGGCTA TATCCTGGGT CTCCTCTCAA
    AGTAGAGAGC CCATCGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_687404.3
    CDS238..2178
    Translation

    Target ORF information:

    RefSeq Version XM_682312.7
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_682312.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCGCACCC AGCACAGTGC CGGTGAGGTG GGCGTCCCGC TGGCAGGAGG AGGCTGCAGT 
    GGTGGCAGCT CAGAGCAGTC GCCTGGCTCC GACTTGGACT GTAGCCCCGC TCTGGGACTG
    GCCGGAGGAG TTCGAGCGAT AAGAGGGGTC ATGTCCCATT ACTGGAGCCT AGAGAGCCTG
    CACTCCACCA ACGCATTGGT GATAACTGAT GGGCCACAGG ATAAGTCTGG TCTTGCAGAT
    GAGGTGGGCA GTGAAGATGA TCTGTACAAT GAATTCCGCA GCTCCTCCAA CCGATTCGGA
    CACCCAGGAG GAGGAGGAGA ACAGCTGGCC ATAAATGAGC TGATCAGTGA TGGCAGTGTG
    TGCGCGGAGG CTCTATGGGA CCACGTCACC ATGGATGACC AGGAGCTGGG CTTTAAAGCT
    GGAGATGTCA TTGAAGTAGT GGACGCTACG AACAAGGAGT GGTGGTGGGG CAGGGTGCTG
    GACAGCGAGG GCTGGTTCCC CGCCAGCTTT GTTCGGTTAC GGGTGAACCA AGATGAGCCC
    ATGGAGGAAT ACCTAGCCCA TCTGGACGGG GCCTCAGAGG GGTGTGGGGC CAGCATGGGG
    GGACCCTTGG GACCTGGACT GCCATGCAAG GAGCAGATGA GAGCCAACGT CATCAATGAG
    ATCATGAGCA CAGAGAGAGA TTACATTAAA CACCTGAAGG ACATATGTGA GGGCTATATA
    AAACAATGTC GGAAGAGGAC AGATATGTTT ACAGAAGAGC AACTGCGCAC TATCTTTGGC
    AACATCGACG AGCTCTACCG ATTCCAGAAG AAGTTCCTCA AAGCCTTGGA GAAAAAATTC
    AACAAAGAGC ATCCTCACCT CAGCGAGATC GGCTCCTGCT TCTTAGAGCA GCAAACAAAC
    TTCCAGATCT ATTCTGAGTA TTGCAACAAC CACCCCAATG CCTGCGTGCA GCTCTCCAAA
    CTGATGAAGA TCAAGAAGTA TGTGTTCTTC TTTGAGGCTT GTCGCCTGCT CCAGAAGATG
    ATTGACATTT CGTTGGATGG ATTCCTACTT ACTCCTGTTC AGAAGATCTG CAAGTATCCT
    CTGCAGCTCG CTGAACTGCT CAAGTACACC AACCCACAGC ACAGAGATTA TAAGGATGTG
    GAAGCTGCCT TAAACGCTAT GAAGAATGTG GCCAGACTGA TCAATGAGAG AAAGAGGCGT
    CTAGAAAATA TTGACAAAAT CGCTCAGTGG CAGAGCTCCA TAGAAGACTG GGAGGGTGAA
    GACGTCTTGA GCAGGAGCTC TGATCTGATC TTTTCTGGAG ACCTGACCAA GATCTCCCAG
    CCACAGGCTA AAGGCCAGCA GAGAATGTTC TTCCTCTTTG ACCACCAACT GGTTTTCTGT
    AAGAAGGATC TGCTTCGGAG GGATATCTTG TACTACAAGG GTCGGTTAGA CATGGATGAG
    ATGGAGGTGG TGGATGTGGA GGATGGGAAG GATAAGGACT TTAATGTGAG TGTGAAGAAT
    GCCTTGAAAT TGTGTTCTCC TGGAGGAGAG GAGGTTCATC TCCTGTGTGC CAAGAAGCCC
    GAACAAAAGC AGCGCTGGCT GAGAGCCTTC ACAGATGAGC GGGAGCAGGT CCAGCATGAC
    CTCGAGACAG GTTTTACGAT TACAGAGGTC CAGAAGAAGC AGGCAATGTT GAATGCAACT
    AAGAGTCATC CAACAGGGAA GCCTAAAGCA TTAGCTTTAC AGCACCTTTT GGCACTCAGA
    GCTCCACTCC TTCACCCCAA AACCAAGTGC CTGCGTTTGT CCGAGCCTCC AGGACCTCTG
    GTTCCCAGGG CCACCCTAAC TTCACCTCTC CTCCCTGAAG GGCCTCAGCG CAGGCAGGGG
    CGGGGGGTTA TGGTGCCCCC TGGGGTCCTG CGCAGGGCTA TATCCTGGGT CTCCTCTCAA
    AGTAGAGAGC CCATCGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06855
    Clone ID Related Accession (Same CDS sequence) XM_682312.7 , XM_682312.8
    Accession Version XM_682312.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_682312.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCGCACCC AGCACAGTGC CGGTGAGGTG GGCGTCCCGC TGGCAGGAGG AGGCTGCAGT 
    GGTGGCAGCT CAGAGCAGTC GCCTGGCTCC GACTTGGACT GTAGCCCCGC TCTGGGACTG
    GCCGGAGGAG TTCGAGCGAT AAGAGGGGTC ATGTCCCATT ACTGGAGCCT AGAGAGCCTG
    CACTCCACCA ACGCATTGGT GATAACTGAT GGGCCACAGG ATAAGTCTGG TCTTGCAGAT
    GAGGTGGGCA GTGAAGATGA TCTGTACAAT GAATTCCGCA GCTCCTCCAA CCGATTCGGA
    CACCCAGGAG GAGGAGGAGA ACAGCTGGCC ATAAATGAGC TGATCAGTGA TGGCAGTGTG
    TGCGCGGAGG CTCTATGGGA CCACGTCACC ATGGATGACC AGGAGCTGGG CTTTAAAGCT
    GGAGATGTCA TTGAAGTAGT GGACGCTACG AACAAGGAGT GGTGGTGGGG CAGGGTGCTG
    GACAGCGAGG GCTGGTTCCC CGCCAGCTTT GTTCGGTTAC GGGTGAACCA AGATGAGCCC
    ATGGAGGAAT ACCTAGCCCA TCTGGACGGG GCCTCAGAGG GGTGTGGGGC CAGCATGGGG
    GGACCCTTGG GACCTGGACT GCCATGCAAG GAGCAGATGA GAGCCAACGT CATCAATGAG
    ATCATGAGCA CAGAGAGAGA TTACATTAAA CACCTGAAGG ACATATGTGA GGGCTATATA
    AAACAATGTC GGAAGAGGAC AGATATGTTT ACAGAAGAGC AACTGCGCAC TATCTTTGGC
    AACATCGACG AGCTCTACCG ATTCCAGAAG AAGTTCCTCA AAGCCTTGGA GAAAAAATTC
    AACAAAGAGC ATCCTCACCT CAGCGAGATC GGCTCCTGCT TCTTAGAGCA GCAAACAAAC
    TTCCAGATCT ATTCTGAGTA TTGCAACAAC CACCCCAATG CCTGCGTGCA GCTCTCCAAA
    CTGATGAAGA TCAAGAAGTA TGTGTTCTTC TTTGAGGCTT GTCGCCTGCT CCAGAAGATG
    ATTGACATTT CGTTGGATGG ATTCCTACTT ACTCCTGTTC AGAAGATCTG CAAGTATCCT
    CTGCAGCTCG CTGAACTGCT CAAGTACACC AACCCACAGC ACAGAGATTA TAAGGATGTG
    GAAGCTGCCT TAAACGCTAT GAAGAATGTG GCCAGACTGA TCAATGAGAG AAAGAGGCGT
    CTAGAAAATA TTGACAAAAT CGCTCAGTGG CAGAGCTCCA TAGAAGACTG GGAGGGTGAA
    GACGTCTTGA GCAGGAGCTC TGATCTGATC TTTTCTGGAG ACCTGACCAA GATCTCCCAG
    CCACAGGCTA AAGGCCAGCA GAGAATGTTC TTCCTCTTTG ACCACCAACT GGTTTTCTGT
    AAGAAGGATC TGCTTCGGAG GGATATCTTG TACTACAAGG GTCGGTTAGA CATGGATGAG
    ATGGAGGTGG TGGATGTGGA GGATGGGAAG GATAAGGACT TTAATGTGAG TGTGAAGAAT
    GCCTTGAAAT TGTGTTCTCC TGGAGGAGAG GAGGTTCATC TCCTGTGTGC CAAGAAGCCC
    GAACAAAAGC AGCGCTGGCT GAGAGCCTTC ACAGATGAGC GGGAGCAGGT CCAGCATGAC
    CTCGAGACAG GTTTTACGAT TACAGAGGTC CAGAAGAAGC AGGCAATGTT GAATGCAACT
    AAGAGTCATC CAACAGGGAA GCCTAAAGCA TTAGCTTTAC AGCACCTTTT GGCACTCAGA
    GCTCCACTCC TTCACCCCAA AACCAAGTGC CTGCGTTTGT CCGAGCCTCC AGGACCTCTG
    GTTCCCAGGG CCACCCTAAC TTCACCTCTC CTCCCTGAAG GGCCTCAGCG CAGGCAGGGG
    CGGGGGGTTA TGGTGCCCCC TGGGGTCCTG CGCAGGGCTA TATCCTGGGT CTCCTCTCAA
    AGTAGAGAGC CCATCGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_687404.3
    CDS276..2216
    Translation

    Target ORF information:

    RefSeq Version XM_682312.8
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_682312.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCGCACCC AGCACAGTGC CGGTGAGGTG GGCGTCCCGC TGGCAGGAGG AGGCTGCAGT 
    GGTGGCAGCT CAGAGCAGTC GCCTGGCTCC GACTTGGACT GTAGCCCCGC TCTGGGACTG
    GCCGGAGGAG TTCGAGCGAT AAGAGGGGTC ATGTCCCATT ACTGGAGCCT AGAGAGCCTG
    CACTCCACCA ACGCATTGGT GATAACTGAT GGGCCACAGG ATAAGTCTGG TCTTGCAGAT
    GAGGTGGGCA GTGAAGATGA TCTGTACAAT GAATTCCGCA GCTCCTCCAA CCGATTCGGA
    CACCCAGGAG GAGGAGGAGA ACAGCTGGCC ATAAATGAGC TGATCAGTGA TGGCAGTGTG
    TGCGCGGAGG CTCTATGGGA CCACGTCACC ATGGATGACC AGGAGCTGGG CTTTAAAGCT
    GGAGATGTCA TTGAAGTAGT GGACGCTACG AACAAGGAGT GGTGGTGGGG CAGGGTGCTG
    GACAGCGAGG GCTGGTTCCC CGCCAGCTTT GTTCGGTTAC GGGTGAACCA AGATGAGCCC
    ATGGAGGAAT ACCTAGCCCA TCTGGACGGG GCCTCAGAGG GGTGTGGGGC CAGCATGGGG
    GGACCCTTGG GACCTGGACT GCCATGCAAG GAGCAGATGA GAGCCAACGT CATCAATGAG
    ATCATGAGCA CAGAGAGAGA TTACATTAAA CACCTGAAGG ACATATGTGA GGGCTATATA
    AAACAATGTC GGAAGAGGAC AGATATGTTT ACAGAAGAGC AACTGCGCAC TATCTTTGGC
    AACATCGACG AGCTCTACCG ATTCCAGAAG AAGTTCCTCA AAGCCTTGGA GAAAAAATTC
    AACAAAGAGC ATCCTCACCT CAGCGAGATC GGCTCCTGCT TCTTAGAGCA GCAAACAAAC
    TTCCAGATCT ATTCTGAGTA TTGCAACAAC CACCCCAATG CCTGCGTGCA GCTCTCCAAA
    CTGATGAAGA TCAAGAAGTA TGTGTTCTTC TTTGAGGCTT GTCGCCTGCT CCAGAAGATG
    ATTGACATTT CGTTGGATGG ATTCCTACTT ACTCCTGTTC AGAAGATCTG CAAGTATCCT
    CTGCAGCTCG CTGAACTGCT CAAGTACACC AACCCACAGC ACAGAGATTA TAAGGATGTG
    GAAGCTGCCT TAAACGCTAT GAAGAATGTG GCCAGACTGA TCAATGAGAG AAAGAGGCGT
    CTAGAAAATA TTGACAAAAT CGCTCAGTGG CAGAGCTCCA TAGAAGACTG GGAGGGTGAA
    GACGTCTTGA GCAGGAGCTC TGATCTGATC TTTTCTGGAG ACCTGACCAA GATCTCCCAG
    CCACAGGCTA AAGGCCAGCA GAGAATGTTC TTCCTCTTTG ACCACCAACT GGTTTTCTGT
    AAGAAGGATC TGCTTCGGAG GGATATCTTG TACTACAAGG GTCGGTTAGA CATGGATGAG
    ATGGAGGTGG TGGATGTGGA GGATGGGAAG GATAAGGACT TTAATGTGAG TGTGAAGAAT
    GCCTTGAAAT TGTGTTCTCC TGGAGGAGAG GAGGTTCATC TCCTGTGTGC CAAGAAGCCC
    GAACAAAAGC AGCGCTGGCT GAGAGCCTTC ACAGATGAGC GGGAGCAGGT CCAGCATGAC
    CTCGAGACAG GTTTTACGAT TACAGAGGTC CAGAAGAAGC AGGCAATGTT GAATGCAACT
    AAGAGTCATC CAACAGGGAA GCCTAAAGCA TTAGCTTTAC AGCACCTTTT GGCACTCAGA
    GCTCCACTCC TTCACCCCAA AACCAAGTGC CTGCGTTTGT CCGAGCCTCC AGGACCTCTG
    GTTCCCAGGG CCACCCTAAC TTCACCTCTC CTCCCTGAAG GGCCTCAGCG CAGGCAGGGG
    CGGGGGGTTA TGGTGCCCCC TGGGGTCCTG CGCAGGGCTA TATCCTGGGT CTCCTCTCAA
    AGTAGAGAGC CCATCGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150441
    Clone ID Related Accession (Same CDS sequence) XM_021467029.1
    Accession Version XM_021467029.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAATCACA GAACTCTGCT GATCACAGCC AGTCACAAGC TTCTTTAAAC
    TGCAGCCAAC CACAACTCTC AGAAGAATTC AGGCCATCAC AAGATTTTGT TGAACACAAC
    CAATCAAATA AAACTGTAGA TCTCAACCAG TCACAGGATT CAGTAAAACA CAGTCAGTTG
    CAGGATACAA TGGAATACAG TAAAACACAA GCCTCAGTAG AGGAGGTTCC AACCAAACAC
    AGCCAGACAG AGAATTTAGT AGAAGACAGG CAATCACAGT ATTCAATAGA ACACAGAAAA
    TCACAGACAT TAGTAGAAGA CAGTCCAACA CATGATTTTG TTGAACTTTG TCAATCAGGG
    CACTTAGCAG AACACAGCCA GTCAGATTGT TTGATAGAAT GCAGTCAATC TACGAATTCA
    GTTAAACACA GCCAATCACA GGACACAGCA GAGCACAACC AATCAGAAGC TTCTGTAATC
    TGCAGTCAAT CAAAGAATTC AATAGAACAC GGTCCATCAC AGGCCTCAGT AGAGAGGTCA
    TCGCAAGATT TTGTTGAAGA CTCACTAGAA CACAGTCAGT CACATGATAT CGCAGAGTGT
    ATTCTGTCGC AAAATGTAAT GGAACACAGC AAATCACAAG TCTTAGATGA TGACAGGCCA
    TCACAAGTTT ATGTTGGAGA CAACCAATCA GATAAATCTG TAGAAGTCAA CATTTCACAA
    GATTCAGAAG AAGACAGGTC ATTAACAGAT TTTGTAGTAC ACAGCCAATC AGAGAACTCA
    GTAGAACACA GCCAGTCACA GGATTTAGTA GAACCTAGGC AATCACATGA TTTAATAGAA
    CACTTCAAAT CACTAGACAA CACAGAGCAC AAGCAATCAG ACATGAGTTA TATAAACTGC
    AGTCAATCAC AGGCCTCAGT AGAAATCTTG CAATCACACG AGTCTGTTGA ACACAGCCAA
    TCAGAACAGT CAGTAAAACA CAGCCAGTCA CAAGACACTG ACAATTTTAG CCAGACACAG
    GATTTAATTA AGCACAGCCA ATCACAGACT TTCATAAAAT GCAAACACAA CCAATCACTG
    GATGGCGTAC AATATGATCA GTCAAAGATC TTAATAGAAC AAACCCAATC ACAGGATTCC
    GTAAAGGACA ATCCATCCCA AGATCTTGTC AAACATAGCC AATCACAGGA CACAGTAGAA
    CCGAGTCAAT CACAGGTCTC AGTAGAACAC ATCCAATCAC AGGATTCCCA AGACTGCAGC
    CATTCACAGA ACTCAACAGT ACACAGCCAA TCACAAACTT CAGTGGGACA AAGCCAATCC
    CAGGATTTGG TAGAACAAAG TCGATCACCA GCTGCAGCCG AAAACAGTCA ATCACAGACT
    TCAGTGGAAC ACAGCCAATC ACTGGATTCA GTGGAACACA TGAAATCTCA GACTTCGGTG
    GAACACAGGC AATCACAGGT TGAATTGATT AAGGCACATT CCATGAACAC CCGGTGGATG
    GTGACTGAGT CAGACACAGT AGCGTTTTAT GACAAGGTGC GATTAAAACA AGACTGCATG
    GATGTTTGCG CAGATTGTGA AGTCGTTGAA TCCTTGGGAG TTTTATCAAA ACATGCTGAC
    AATTTGAACT TCGAAAATAA CGAAAGACTA AATTTCAAAA CACAGGACTT AAATGACACT
    TCACCTGTTG TTTCTGAATA CGAGTGTTTC CCACATGTGC CTAATGCATC TTCTATTGGA
    GTGCCAGATT CACTGCATCA GTGCACGATT AGCAATTCAC CCTCAGACCT GCAGGTTAAA
    ACTCAGCAGA GTTGGCCAGT ATTACAAATA CACACCGAAA AAGACGATTA CTCAGTTAGT
    AGGTATCACA TGGATGGTTA CTGTTTGCAG GACAGTGAGC AAACAGAGTT TTACAAAACC
    TACCAGTGCC CTAACAGTAC CATTAATTTT AATACACAGG GTGAGTTCAC TGAAGCTCGG
    AAGGTTGTAT CAACTGAAGG TGTATTTACT AGAGGTTGTA TCAACACCAA ACTTTTTGAT
    GGCAAAAGTG TTGACATTGT AGGAGGACCG CAGAAGGACA GCAACAAGCA TCACGTTATG
    AAAGGGAAAG AATGTACAAT AGTTCCTTCA ATAGAGCAGT CAGTAATTTA CTCTGACAGT
    TCAGGATGCA TAGAATATAA AAGGCCCAAA ACGTTGAGCA TTGCTTGTCC TGACATGACG
    ACTGCTGAAT CTGAAGGGTG TCCTGTAGAC AGTCCTGTTG AATCACTTTC TACACTGTTT
    GAGATTCCAC ATGACAGATC TACAGTCAGC CCCACAGACA TTTGCTTTGA GTTTAGTGAT
    GGCCCTCATA TGAACTGTAC ACAAGTAGCA CAGAGTGTTA TAGAATATTC AGATGCCCCC
    AAACTATTTC ACCAGTTTGA CACCCTGTCT GACAAACAAA GAAGACCTAA TGAGTCAGCT
    GAAGCAATTG CGTCGAATGT TGATATTTTG TCTGGTGCAC GTTCCGATCC TTTCACAAAT
    GCACAGACTC CTTTTGATTA TTCCCAGGTG AACAGCAGGA ACTATACACA GAGCTCAGAC
    ACAAATAATA ACATTAAAGC CATTACTTTA GAAGAGTGTG CCTCGGGAGT ACAAATGTCA
    TTGCCTAGTT CATTGCAGAA ATACAGTAGG TTTACAAATA ATGCTGAATG CTTAGTCTCA
    AAAAGGGGTG ATTACAAAAA CATTGATTCT TTTAGTGCTC GTAATACTCT GGCTACCCCC
    TTCAAAGCAC CTGAAATGAA GACTGAAGTG AACAGCAGAG AAAATGTTGT GAAATCTAGC
    AGTCACATAG ATGTTACACA GAGGAAGCTC TGTGATTGCT GCGGTGGAAA GGAGTGCCAG
    CTGCAGACAG CGGGTGTATT TCAGTTCAAG GGCACAGACG CCATCAGTCA GCGCAGAACT
    GCAGTGTTGC AAACCTTGAG TCCCCTCCAC ACAGATGACA GCACACATGA AGCGGTAAAA
    GTAGACTCTA CAAGCTGTGA ATTTTCACAT ATGGTCAGTT TGCTTACACA CAAACATCCG
    AATCTTGTCC AAGAAGACTT CGTATTACCA CTGACTGGAG CTCATAAGGC TGATGATACT
    CATGTATTAG AAAACATGGA GAGAAAGCAT GAAACTTTCC CAATAAATGC TCTTAAAAGC
    ACTGAATCAA TATGTGAAAC TTCTGATCCT CTTTTTAAGC AAACATTGCA ATTGCACAAC
    TCGGAGGTTG AGGGCGCATT TGTTTCATGT TGTCATATTT TGCCCATCAC CAACTTAGAG
    GCCATTTTAG AGACGGACCG TTCCCCGGAG AGCTCATTTG CAGTAGAAAA TGAACTGGAT
    GAGCAACAGA GAGGAAGTCC TTTATCAGAA GGTGGATTAA ATGAGGACCT AAGCTCTTGT
    GAGGATGTCA AAAACCGCCC ACATGACAGC AGTTGTGATA TGCATACAAA AGCCAATGTA
    GCCAATTTCA TGAATGAACC CGATGCTGGA GAAACAATGA ACTCTTTATC AAATGGGTTT
    GACAGCAGTG ATGACCATAG TGTTTCCAGT TTGGTGTCAC AAGACCTTGA CTCCAGTACC
    AACAGTGAGG AGACGGAAGA CACCATGCAG CCATCGTTTG GTCTTCGTGG TAATGCGAGA
    AATAAAAGAA CACAGTCAGC CAAGGGCTCT ATGTTTTCTG GTTTGATTCG AATGCCCACC
    TTCCGCAAGC CAAAACGAGA ACTCAAACGC AATAAAAACA GGGTTGATCC GAAAGTTAGG
    GCATCCAGTG GGGATGGAGA TGAACAAGAT GAGATGAAGA ACAACAGGTC ACCCACATGC
    AGCAAATCTC ACTCTTCACT GTACAAGACC CCTATATCTC AAAGTGCAGA TCACCTTACT
    GATATTACAA AGCATTCTGA CCACTCAAAT GATGATGTTT TTGAAAGGGC TTTTGCTTTA
    AATGTGCAAA ATAGAGAGAA ACACATGCAA CCTCAACACC CGTTTGAGCA GCAGCAGCAG
    AAAGATGGAG ATGCACTCAG TTTTAGAGCT GGCCAGACAA CAGAGGGGTT GCAACAAAAA
    AGATGCAAGA GCATTGATAA CTTAAACTTA CGCATGAAGC TGGCATTGGC TCACAAGTCT
    ATTTCTAGCC TGTTTGAGAC CAGATATCCT GAGAGGGACC AAGAGCAAAT CTTACAGTTA
    GAGAATGAGG ATGGGAAAAC CAAGCAATCA TGGAGAAAGA CCAAACGATG TAACGAAGCA
    GAACTTTGCA AAAGAACTCC TGTTCCTGGG ACGGTCTGTG ACAAGTGCAT ACATCAGAGC
    CAAATTGATT GTGCCTTCTG TGCTTATCAG AACCGGTCCA TATTAACCAA CTTACCAAAT
    GTCCAAGCAA GATTGAAGAA CACGTGCCAT TCTGACCCGC AGAGTTTGAA ATCTGCACCT
    CAGGAAGGGT TTGAAGATGG TAAATATCTG GAGGAAGGTG AGCAGCCTTG TGGTGCTTTC
    TCAGATTCAG ACCAAGCATC GGTAGATGGT TTTGAGTCCA GTGTAGAGGA CAGGTCGCTG
    TCACCTGCAC ACCCCACTGT TCTTGCACTT TCAAACCAAA TGTCACCTAC CTGGACAAGG
    TCCCTAAGCT GCTTCGAGAC AACTGATACA CCGACAAGGC CCATGAGCCC CAAACCACAC
    AGTCCTGGTC TGGGCTGGAC ACAGCGGAGG AGCTTCCGCT ACCCCTCAAG ATCTGTTGCA
    TCTTCTCTCT GCTTGCTTGG TCAGGGAGTG AGCACTGACG GTCTCTTAGA CCCACCTCAG
    AGGCCGACAT CTCTAAAACC AAGGACAACA CAACTCACCA CAGCTCACTC CTTCGATTCT
    GAGTACTTAC TGGAGGACAG CAGTTCAGAC AACCAGTCTC AGAATAGCCT GAATTCAGCC
    TCCCTGATCA GCAAAACAGA GCATGTACAG CACATTACTG AAGCAGAGAG AAGACCACAA
    GAAAATGCAT GTGTGCTTCG GGTGAAGTCC AGAGATCCAG GTACAGCTGC GCCCAGGCCT
    ATGTCAGACC TGTACGGCTG GACCATGTCT CTTCAGGGCA CCAGAGAGTT GGCGCTCGAC
    TTGGAGAGGA AGACTAACGC ACATCAGCGC TCATGCTCAT ATGAAACATT GACCGATGTG
    GATAACATCC GCAAAAAGAA GCTAAGCAAG CAGCAAAGTT TGCGGCAGAT TAGCACATCA
    ACATCTGAGA GGCATGAGAA ATTGCGAGCT AGACTTTCCC TCACATCCCC GGAGCAGTTC
    CCCTCTGTTC CTCTCAAAGA CCACTTCTTC TCTCAGAGCA CACCTGTTGG GCTGGACTAT
    CTGGGCTGGG CTCACCGTGC ATCCTTTTCA GAAACGAACC TCAGCGCACC GGAAAAAGAG
    GCACATGGAC AGGTCGCACC TCTCGCATTG GTGATAACTG ATGGGCCACA GGATAAGTCT
    GGTCTTGCAG ATGAGGTGGG CAGTGAAGAT GATCTGTACA ATGAATTCCG CAGCTCCTCC
    AACCGATTCG GACACCCAGG AGGAGGAGGA GAACAGCTGG CCATAAATGA GCTGATCAGT
    GATGGCAGTG TGTGCGCGGA GGCTCTATGG GACCACGTCA CCATGGATGA CCAGGAGCTG
    GGCTTTAAAG CTGGAGATGT CATTGAAGTA GTGGACGCTA CGAACAAGGA GTGGTGGTGG
    GGCAGGGTGC TGGACAGCGA GGGCTGGTTC CCCGCCAGCT TTGTTCGGTT ACGGGTGAAC
    CAAGATGAGC CCATGGAGGA ATACCTAGCC CATCTGGACG GGGCCTCAGA GGGGTGTGGG
    GCCAGCATGG GGGGACCCTT GGGACCTGGA CTGCCATGCA AGGAGCAGAT GAGAGCCAAC
    GTCATCAATG AGATCATGAG CACAGAGAGA GATTACATTA AACACCTGAA GGACATATGT
    GAGGGCTATA TAAAACAATG TCGGAAGAGG ACAGATATGT TTACAGAAGA GCAACTGCGC
    ACTATCTTTG GCAACATCGA CGAGCTCTAC CGATTCCAGA AGAAGTTCCT CAAAGCCTTG
    GAGAAAAAAT TCAACAAAGA GCATCCTCAC CTCAGCGAGA TCGGCTCCTG CTTCTTAGAG
    CAGCAAACAA ACTTCCAGAT CTATTCTGAG TATTGCAACA ACCACCCCAA TGCCTGCGTG
    CAGCTCTCCA AACTGATGAA GATCAAGAAG TATGTGTTCT TCTTTGAGGC TTGTCGCCTG
    CTCCAGAAGA TGATTGACAT TTCGTTGGAT GGATTCCTAC TTACTCCTGT TCAGAAGATC
    TGCAAGTATC CTCTGCAGCT CGCTGAACTG CTCAAGTACA CCAACCCACA GCACAGAGAT
    TATAAGGATG TGGAAGCTGC CTTAAACGCT ATGAAGAATG TGGCCAGACT GATCAATGAG
    AGAAAGAGGC GTCTAGAAAA TATTGACAAA ATCGCTCAGT GGCAGAGCTC CATAGAAGAC
    TGGGAGGGTG AAGACGTCTT GAGCAGGAGC TCTGATCTGA TCTTTTCTGG AGACCTGACC
    AAGATCTCCC AGCCACAGGC TAAAGGCCAG CAGAGAATGT TCTTCCTCTT TGACCACCAA
    CTGGTTTTCT GTAAGAAGGA TCTGCTTCGG AGGGATATCT TGTACTACAA GGGTCGGTTA
    GACATGGATG AGATGGAGGT GGTGGATGTG GAGGATGGGA AGGATAAGGA CTTTAATGTG
    AGTGTGAAGA ATGCCTTGAA ATTGTGTTCT CCTGGAGGAG AGGAGGTTCA TCTCCTGTGT
    GCCAAGAAGC CCGAACAAAA GCAGCGCTGG CTGAGAGCCT TCACAGATGA GCGGGAGCAG
    GTCCAGCATG ACCTCGAGAC AGGTTTTACG ATTACAGAGG TCCAGAAGAA GCAGGCAATG
    TTGAATGCAA CTAAGAGTCA TCCAACAGGG AAGCCTAAAG CATTAGCTTT ACAGCACCTT
    TTGGCACTCA GAGCTCCACT CCTTCACCCC AAAACCAAGT GCCTGCGTTT GTCCGAGCCT
    CCAGGACCTC TGGTTCCCAG GGCCACCCTA ACTTCACCTC TCCTCCCTGA AGGGCCTCAG
    CGCAGGCAGG GGCGGGGGGT TATGGTGCCC CCTGGGGTCC TGCGCAGGGC TATATCCTGG
    GTCTCCTCTC AAAGTAGAGA GCCCATCGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322704.1
    CDS347..9559
    Translation

    Target ORF information:

    RefSeq Version XM_021467029.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467029.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAATCACA GAACTCTGCT GATCACAGCC AGTCACAAGC TTCTTTAAAC
    TGCAGCCAAC CACAACTCTC AGAAGAATTC AGGCCATCAC AAGATTTTGT TGAACACAAC
    CAATCAAATA AAACTGTAGA TCTCAACCAG TCACAGGATT CAGTAAAACA CAGTCAGTTG
    CAGGATACAA TGGAATACAG TAAAACACAA GCCTCAGTAG AGGAGGTTCC AACCAAACAC
    AGCCAGACAG AGAATTTAGT AGAAGACAGG CAATCACAGT ATTCAATAGA ACACAGAAAA
    TCACAGACAT TAGTAGAAGA CAGTCCAACA CATGATTTTG TTGAACTTTG TCAATCAGGG
    CACTTAGCAG AACACAGCCA GTCAGATTGT TTGATAGAAT GCAGTCAATC TACGAATTCA
    GTTAAACACA GCCAATCACA GGACACAGCA GAGCACAACC AATCAGAAGC TTCTGTAATC
    TGCAGTCAAT CAAAGAATTC AATAGAACAC GGTCCATCAC AGGCCTCAGT AGAGAGGTCA
    TCGCAAGATT TTGTTGAAGA CTCACTAGAA CACAGTCAGT CACATGATAT CGCAGAGTGT
    ATTCTGTCGC AAAATGTAAT GGAACACAGC AAATCACAAG TCTTAGATGA TGACAGGCCA
    TCACAAGTTT ATGTTGGAGA CAACCAATCA GATAAATCTG TAGAAGTCAA CATTTCACAA
    GATTCAGAAG AAGACAGGTC ATTAACAGAT TTTGTAGTAC ACAGCCAATC AGAGAACTCA
    GTAGAACACA GCCAGTCACA GGATTTAGTA GAACCTAGGC AATCACATGA TTTAATAGAA
    CACTTCAAAT CACTAGACAA CACAGAGCAC AAGCAATCAG ACATGAGTTA TATAAACTGC
    AGTCAATCAC AGGCCTCAGT AGAAATCTTG CAATCACACG AGTCTGTTGA ACACAGCCAA
    TCAGAACAGT CAGTAAAACA CAGCCAGTCA CAAGACACTG ACAATTTTAG CCAGACACAG
    GATTTAATTA AGCACAGCCA ATCACAGACT TTCATAAAAT GCAAACACAA CCAATCACTG
    GATGGCGTAC AATATGATCA GTCAAAGATC TTAATAGAAC AAACCCAATC ACAGGATTCC
    GTAAAGGACA ATCCATCCCA AGATCTTGTC AAACATAGCC AATCACAGGA CACAGTAGAA
    CCGAGTCAAT CACAGGTCTC AGTAGAACAC ATCCAATCAC AGGATTCCCA AGACTGCAGC
    CATTCACAGA ACTCAACAGT ACACAGCCAA TCACAAACTT CAGTGGGACA AAGCCAATCC
    CAGGATTTGG TAGAACAAAG TCGATCACCA GCTGCAGCCG AAAACAGTCA ATCACAGACT
    TCAGTGGAAC ACAGCCAATC ACTGGATTCA GTGGAACACA TGAAATCTCA GACTTCGGTG
    GAACACAGGC AATCACAGGT TGAATTGATT AAGGCACATT CCATGAACAC CCGGTGGATG
    GTGACTGAGT CAGACACAGT AGCGTTTTAT GACAAGGTGC GATTAAAACA AGACTGCATG
    GATGTTTGCG CAGATTGTGA AGTCGTTGAA TCCTTGGGAG TTTTATCAAA ACATGCTGAC
    AATTTGAACT TCGAAAATAA CGAAAGACTA AATTTCAAAA CACAGGACTT AAATGACACT
    TCACCTGTTG TTTCTGAATA CGAGTGTTTC CCACATGTGC CTAATGCATC TTCTATTGGA
    GTGCCAGATT CACTGCATCA GTGCACGATT AGCAATTCAC CCTCAGACCT GCAGGTTAAA
    ACTCAGCAGA GTTGGCCAGT ATTACAAATA CACACCGAAA AAGACGATTA CTCAGTTAGT
    AGGTATCACA TGGATGGTTA CTGTTTGCAG GACAGTGAGC AAACAGAGTT TTACAAAACC
    TACCAGTGCC CTAACAGTAC CATTAATTTT AATACACAGG GTGAGTTCAC TGAAGCTCGG
    AAGGTTGTAT CAACTGAAGG TGTATTTACT AGAGGTTGTA TCAACACCAA ACTTTTTGAT
    GGCAAAAGTG TTGACATTGT AGGAGGACCG CAGAAGGACA GCAACAAGCA TCACGTTATG
    AAAGGGAAAG AATGTACAAT AGTTCCTTCA ATAGAGCAGT CAGTAATTTA CTCTGACAGT
    TCAGGATGCA TAGAATATAA AAGGCCCAAA ACGTTGAGCA TTGCTTGTCC TGACATGACG
    ACTGCTGAAT CTGAAGGGTG TCCTGTAGAC AGTCCTGTTG AATCACTTTC TACACTGTTT
    GAGATTCCAC ATGACAGATC TACAGTCAGC CCCACAGACA TTTGCTTTGA GTTTAGTGAT
    GGCCCTCATA TGAACTGTAC ACAAGTAGCA CAGAGTGTTA TAGAATATTC AGATGCCCCC
    AAACTATTTC ACCAGTTTGA CACCCTGTCT GACAAACAAA GAAGACCTAA TGAGTCAGCT
    GAAGCAATTG CGTCGAATGT TGATATTTTG TCTGGTGCAC GTTCCGATCC TTTCACAAAT
    GCACAGACTC CTTTTGATTA TTCCCAGGTG AACAGCAGGA ACTATACACA GAGCTCAGAC
    ACAAATAATA ACATTAAAGC CATTACTTTA GAAGAGTGTG CCTCGGGAGT ACAAATGTCA
    TTGCCTAGTT CATTGCAGAA ATACAGTAGG TTTACAAATA ATGCTGAATG CTTAGTCTCA
    AAAAGGGGTG ATTACAAAAA CATTGATTCT TTTAGTGCTC GTAATACTCT GGCTACCCCC
    TTCAAAGCAC CTGAAATGAA GACTGAAGTG AACAGCAGAG AAAATGTTGT GAAATCTAGC
    AGTCACATAG ATGTTACACA GAGGAAGCTC TGTGATTGCT GCGGTGGAAA GGAGTGCCAG
    CTGCAGACAG CGGGTGTATT TCAGTTCAAG GGCACAGACG CCATCAGTCA GCGCAGAACT
    GCAGTGTTGC AAACCTTGAG TCCCCTCCAC ACAGATGACA GCACACATGA AGCGGTAAAA
    GTAGACTCTA CAAGCTGTGA ATTTTCACAT ATGGTCAGTT TGCTTACACA CAAACATCCG
    AATCTTGTCC AAGAAGACTT CGTATTACCA CTGACTGGAG CTCATAAGGC TGATGATACT
    CATGTATTAG AAAACATGGA GAGAAAGCAT GAAACTTTCC CAATAAATGC TCTTAAAAGC
    ACTGAATCAA TATGTGAAAC TTCTGATCCT CTTTTTAAGC AAACATTGCA ATTGCACAAC
    TCGGAGGTTG AGGGCGCATT TGTTTCATGT TGTCATATTT TGCCCATCAC CAACTTAGAG
    GCCATTTTAG AGACGGACCG TTCCCCGGAG AGCTCATTTG CAGTAGAAAA TGAACTGGAT
    GAGCAACAGA GAGGAAGTCC TTTATCAGAA GGTGGATTAA ATGAGGACCT AAGCTCTTGT
    GAGGATGTCA AAAACCGCCC ACATGACAGC AGTTGTGATA TGCATACAAA AGCCAATGTA
    GCCAATTTCA TGAATGAACC CGATGCTGGA GAAACAATGA ACTCTTTATC AAATGGGTTT
    GACAGCAGTG ATGACCATAG TGTTTCCAGT TTGGTGTCAC AAGACCTTGA CTCCAGTACC
    AACAGTGAGG AGACGGAAGA CACCATGCAG CCATCGTTTG GTCTTCGTGG TAATGCGAGA
    AATAAAAGAA CACAGTCAGC CAAGGGCTCT ATGTTTTCTG GTTTGATTCG AATGCCCACC
    TTCCGCAAGC CAAAACGAGA ACTCAAACGC AATAAAAACA GGGTTGATCC GAAAGTTAGG
    GCATCCAGTG GGGATGGAGA TGAACAAGAT GAGATGAAGA ACAACAGGTC ACCCACATGC
    AGCAAATCTC ACTCTTCACT GTACAAGACC CCTATATCTC AAAGTGCAGA TCACCTTACT
    GATATTACAA AGCATTCTGA CCACTCAAAT GATGATGTTT TTGAAAGGGC TTTTGCTTTA
    AATGTGCAAA ATAGAGAGAA ACACATGCAA CCTCAACACC CGTTTGAGCA GCAGCAGCAG
    AAAGATGGAG ATGCACTCAG TTTTAGAGCT GGCCAGACAA CAGAGGGGTT GCAACAAAAA
    AGATGCAAGA GCATTGATAA CTTAAACTTA CGCATGAAGC TGGCATTGGC TCACAAGTCT
    ATTTCTAGCC TGTTTGAGAC CAGATATCCT GAGAGGGACC AAGAGCAAAT CTTACAGTTA
    GAGAATGAGG ATGGGAAAAC CAAGCAATCA TGGAGAAAGA CCAAACGATG TAACGAAGCA
    GAACTTTGCA AAAGAACTCC TGTTCCTGGG ACGGTCTGTG ACAAGTGCAT ACATCAGAGC
    CAAATTGATT GTGCCTTCTG TGCTTATCAG AACCGGTCCA TATTAACCAA CTTACCAAAT
    GTCCAAGCAA GATTGAAGAA CACGTGCCAT TCTGACCCGC AGAGTTTGAA ATCTGCACCT
    CAGGAAGGGT TTGAAGATGG TAAATATCTG GAGGAAGGTG AGCAGCCTTG TGGTGCTTTC
    TCAGATTCAG ACCAAGCATC GGTAGATGGT TTTGAGTCCA GTGTAGAGGA CAGGTCGCTG
    TCACCTGCAC ACCCCACTGT TCTTGCACTT TCAAACCAAA TGTCACCTAC CTGGACAAGG
    TCCCTAAGCT GCTTCGAGAC AACTGATACA CCGACAAGGC CCATGAGCCC CAAACCACAC
    AGTCCTGGTC TGGGCTGGAC ACAGCGGAGG AGCTTCCGCT ACCCCTCAAG ATCTGTTGCA
    TCTTCTCTCT GCTTGCTTGG TCAGGGAGTG AGCACTGACG GTCTCTTAGA CCCACCTCAG
    AGGCCGACAT CTCTAAAACC AAGGACAACA CAACTCACCA CAGCTCACTC CTTCGATTCT
    GAGTACTTAC TGGAGGACAG CAGTTCAGAC AACCAGTCTC AGAATAGCCT GAATTCAGCC
    TCCCTGATCA GCAAAACAGA GCATGTACAG CACATTACTG AAGCAGAGAG AAGACCACAA
    GAAAATGCAT GTGTGCTTCG GGTGAAGTCC AGAGATCCAG GTACAGCTGC GCCCAGGCCT
    ATGTCAGACC TGTACGGCTG GACCATGTCT CTTCAGGGCA CCAGAGAGTT GGCGCTCGAC
    TTGGAGAGGA AGACTAACGC ACATCAGCGC TCATGCTCAT ATGAAACATT GACCGATGTG
    GATAACATCC GCAAAAAGAA GCTAAGCAAG CAGCAAAGTT TGCGGCAGAT TAGCACATCA
    ACATCTGAGA GGCATGAGAA ATTGCGAGCT AGACTTTCCC TCACATCCCC GGAGCAGTTC
    CCCTCTGTTC CTCTCAAAGA CCACTTCTTC TCTCAGAGCA CACCTGTTGG GCTGGACTAT
    CTGGGCTGGG CTCACCGTGC ATCCTTTTCA GAAACGAACC TCAGCGCACC GGAAAAAGAG
    GCACATGGAC AGGTCGCACC TCTCGCATTG GTGATAACTG ATGGGCCACA GGATAAGTCT
    GGTCTTGCAG ATGAGGTGGG CAGTGAAGAT GATCTGTACA ATGAATTCCG CAGCTCCTCC
    AACCGATTCG GACACCCAGG AGGAGGAGGA GAACAGCTGG CCATAAATGA GCTGATCAGT
    GATGGCAGTG TGTGCGCGGA GGCTCTATGG GACCACGTCA CCATGGATGA CCAGGAGCTG
    GGCTTTAAAG CTGGAGATGT CATTGAAGTA GTGGACGCTA CGAACAAGGA GTGGTGGTGG
    GGCAGGGTGC TGGACAGCGA GGGCTGGTTC CCCGCCAGCT TTGTTCGGTT ACGGGTGAAC
    CAAGATGAGC CCATGGAGGA ATACCTAGCC CATCTGGACG GGGCCTCAGA GGGGTGTGGG
    GCCAGCATGG GGGGACCCTT GGGACCTGGA CTGCCATGCA AGGAGCAGAT GAGAGCCAAC
    GTCATCAATG AGATCATGAG CACAGAGAGA GATTACATTA AACACCTGAA GGACATATGT
    GAGGGCTATA TAAAACAATG TCGGAAGAGG ACAGATATGT TTACAGAAGA GCAACTGCGC
    ACTATCTTTG GCAACATCGA CGAGCTCTAC CGATTCCAGA AGAAGTTCCT CAAAGCCTTG
    GAGAAAAAAT TCAACAAAGA GCATCCTCAC CTCAGCGAGA TCGGCTCCTG CTTCTTAGAG
    CAGCAAACAA ACTTCCAGAT CTATTCTGAG TATTGCAACA ACCACCCCAA TGCCTGCGTG
    CAGCTCTCCA AACTGATGAA GATCAAGAAG TATGTGTTCT TCTTTGAGGC TTGTCGCCTG
    CTCCAGAAGA TGATTGACAT TTCGTTGGAT GGATTCCTAC TTACTCCTGT TCAGAAGATC
    TGCAAGTATC CTCTGCAGCT CGCTGAACTG CTCAAGTACA CCAACCCACA GCACAGAGAT
    TATAAGGATG TGGAAGCTGC CTTAAACGCT ATGAAGAATG TGGCCAGACT GATCAATGAG
    AGAAAGAGGC GTCTAGAAAA TATTGACAAA ATCGCTCAGT GGCAGAGCTC CATAGAAGAC
    TGGGAGGGTG AAGACGTCTT GAGCAGGAGC TCTGATCTGA TCTTTTCTGG AGACCTGACC
    AAGATCTCCC AGCCACAGGC TAAAGGCCAG CAGAGAATGT TCTTCCTCTT TGACCACCAA
    CTGGTTTTCT GTAAGAAGGA TCTGCTTCGG AGGGATATCT TGTACTACAA GGGTCGGTTA
    GACATGGATG AGATGGAGGT GGTGGATGTG GAGGATGGGA AGGATAAGGA CTTTAATGTG
    AGTGTGAAGA ATGCCTTGAA ATTGTGTTCT CCTGGAGGAG AGGAGGTTCA TCTCCTGTGT
    GCCAAGAAGC CCGAACAAAA GCAGCGCTGG CTGAGAGCCT TCACAGATGA GCGGGAGCAG
    GTCCAGCATG ACCTCGAGAC AGGTTTTACG ATTACAGAGG TCCAGAAGAA GCAGGCAATG
    TTGAATGCAA CTAAGAGTCA TCCAACAGGG AAGCCTAAAG CATTAGCTTT ACAGCACCTT
    TTGGCACTCA GAGCTCCACT CCTTCACCCC AAAACCAAGT GCCTGCGTTT GTCCGAGCCT
    CCAGGACCTC TGGTTCCCAG GGCCACCCTA ACTTCACCTC TCCTCCCTGA AGGGCCTCAG
    CGCAGGCAGG GGCGGGGGGT TATGGTGCCC CCTGGGGTCC TGCGCAGGGC TATATCCTGG
    GTCTCCTCTC AAAGTAGAGA GCCCATCGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150442
    Clone ID Related Accession (Same CDS sequence) XM_021467031.1
    Accession Version XM_021467031.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA ACCACAGATA
    TCAGTAGAGC ACAGCCAACC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAT
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAT TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA TCCACAGATA TCAGTAGAGC ACAGCCGAAC ACATATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAACC AACCACAGAT GTCAGTAGAG
    CACAACCAAC CGCAGATGTC AGTAGACCAC AGCCAACCAC AGATATCAGT AGACCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGCAGAGCA CAGGCATTCA
    CAGATGTCAG TAGAGCACAG CCAAGCACAG ATGTCAGTAG ACCACAGTCA ACCACAGATG
    TCAGTAGGCC ACAGTCAATC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATGTC AGCAGAGCAC
    AGGCAATCAC AGATGTCAGT AGAGCACAGC CAACCTCAGA TGTTAGTAGA GCACAGCCAA
    TCACAGATGT CAGTAGAGCA CAGCCAATCA CAGATGTCAG TAGAGCACAG CCAATCGCAG
    ATGTCAGTAG AGCACAGCCA ACCACAGATG TTAGTAGAGC ACAGCCAACC ACAGTTATTT
    GTAGAGCACA GCCAATCACA GAACTCTGCT GATCACAGCC AGTCACAAGC TTCTTTAAAC
    TGCAGCCAAC CACAACTCTC AGAAGAATTC AGGCCATCAC AAGATTTTGT TGAACACAAC
    CAATCAAATA AAACTGTAGA TCTCAACCAG TCACAGGATT CAGTAAAACA CAGTCAGTTG
    CAGGATACAA TGGAATACAG TAAAACACAA GCCTCAGTAG AGGAGGTTCC AACCAAACAC
    AGCCAGACAG AGAATTTAGT AGAAGACAGG CAATCACAGT ATTCAATAGA ACACAGAAAA
    TCACAGACAT TAGTAGAAGA CAGTCCAACA CATGATTTTG TTGAACTTTG TCAATCAGGG
    CACTTAGCAG AACACAGCCA GTCAGATTGT TTGATAGAAT GCAGTCAATC TACGAATTCA
    GTTAAACACA GCCAATCACA GGACACAGCA GAGCACAACC AATCAGAAGC TTCTGTAATC
    TGCAGTCAAT CAAAGAATTC AATAGAACAC GGTCCATCAC AGGCCTCAGT AGAGAGGTCA
    TCGCAAGATT TTGTTGAAGA CTCACTAGAA CACAGTCAGT CACATGATAT CGCAGAGTGT
    ATTCTGTCGC AAAATGTAAT GGAACACAGC AAATCACAAG TCTTAGATGA TGACAGGCCA
    TCACAAGTTT ATGTTGGAGA CAACCAATCA GATAAATCTG TAGAAGTCAA CATTTCACAA
    GATTCAGAAG AAGACAGGTC ATTAACAGAT TTTGTAGTAC ACAGCCAATC AGAGAACTCA
    GTAGAACACA GCCAGTCACA GGATTTAGTA GAACCTAGGC AATCACATGA TTTAATAGAA
    CACTTCAAAT CACTAGACAA CACAGAGCAC AAGCAATCAG ACATGAGTTA TATAAACTGC
    AGTCAATCAC AGGCCTCAGT AGAAATCTTG CAATCACACG AGTCTGTTGA ACACAGCCAA
    TCAGAACAGT CAGTAAAACA CAGCCAGTCA CAAGACACTG ACAATTTTAG CCAGACACAG
    GATTTAATTA AGCACAGCCA ATCACAGACT TTCATAAAAT GCAAACACAA CCAATCACTG
    GATGGCGTAC AATATGATCA GTCAAAGATC TTAATAGAAC AAACCCAATC ACAGGATTCC
    GTAAAGGACA ATCCATCCCA AGATCTTGTC AAACATAGCC AATCACAGGA CACAGTAGAA
    CCGAGTCAAT CACAGGTCTC AGTAGAACAC ATCCAATCAC AGGATTCCCA AGACTGCAGC
    CATTCACAGA ACTCAACAGT ACACAGCCAA TCACAAACTT CAGTGGGACA AAGCCAATCC
    CAGGATTTGG TAGAACAAAG TCGATCACCA GCTGCAGCCG AAAACAGTCA ATCACAGACT
    TCAGTGGAAC ACAGCCAATC ACTGGATTCA GTGGAACACA TGAAATCTCA GACTTCGGTG
    GAACACAGGC AATCACAGGT TGAATTGATT AAGGCACATT CCATGAACAC CCGGTGGATG
    GTGACTGAGT CAGACACAGT AGCGTTTTAT GACAAGGTGC GATTAAAACA AGACTGCATG
    GATGTTTGCG CAGATTGTGA AGTCGTTGAA TCCTTGGGAG TTTTATCAAA ACATGCTGAC
    AATTTGAACT TCGAAAATAA CGAAAGACTA AATTTCAAAA CACAGGACTT AAATGACACT
    TCACCTGTTG TTTCTGAATA CGAGTGTTTC CCACATGTGC CTAATGCATC TTCTATTGGA
    GTGCCAGATT CACTGCATCA GTGCACGATT AGCAATTCAC CCTCAGACCT GCAGGTTAAA
    ACTCAGCAGA GTTGGCCAGT ATTACAAATA CACACCGAAA AAGACGATTA CTCAGTTAGT
    AGGTATCACA TGGATGGTTA CTGTTTGCAG GACAGTGAGC AAACAGAGTT TTACAAAACC
    TACCAGTGCC CTAACAGTAC CATTAATTTT AATACACAGG GTGAGTTCAC TGAAGCTCGG
    AAGGTTGTAT CAACTGAAGG TGTATTTACT AGAGGTTGTA TCAACACCAA ACTTTTTGAT
    GGCAAAAGTG TTGACATTGT AGGAGGACCG CAGAAGGACA GCAACAAGCA TCACGTTATG
    AAAGGGAAAG AATGTACAAT AGTTCCTTCA ATAGAGCAGT CAGTAATTTA CTCTGACAGT
    TCAGGATGCA TAGAATATAA AAGGCCCAAA ACGTTGAGCA TTGCTTGTCC TGACATGACG
    ACTGCTGAAT CTGAAGGGTG TCCTGTAGAC AGTCCTGTTG AATCACTTTC TACACTGTTT
    GAGATTCCAC ATGACAGATC TACAGTCAGC CCCACAGACA TTTGCTTTGA GTTTAGTGAT
    GGCCCTCATA TGAACTGTAC ACAAGTAGCA CAGAGTGTTA TAGAATATTC AGATGCCCCC
    AAACTATTTC ACCAGTTTGA CACCCTGTCT GACAAACAAA GAAGACCTAA TGAGTCAGCT
    GAAGCAATTG CGTCGAATGT TGATATTTTG TCTGGTGCAC GTTCCGATCC TTTCACAAAT
    GCACAGACTC CTTTTGATTA TTCCCAGGTG AACAGCAGGA ACTATACACA GAGCTCAGAC
    ACAAATAATA ACATTAAAGC CATTACTTTA GAAGAGTGTG CCTCGGGAGT ACAAATGTCA
    TTGCCTAGTT CATTGCAGAA ATACAGTAGG TTTACAAATA ATGCTGAATG CTTAGTCTCA
    AAAAGGGGTG ATTACAAAAA CATTGATTCT TTTAGTGCTC GTAATACTCT GGCTACCCCC
    TTCAAAGCAC CTGAAATGAA GACTGAAGTG AACAGCAGAG AAAATGTTGT GAAATCTAGC
    AGTCACATAG ATGTTACACA GAGGAAGCTC TGTGATTGCT GCGGTGGAAA GGAGTGCCAG
    CTGCAGACAG CGGGTGTATT TCAGTTCAAG GGCACAGACG CCATCAGTCA GCGCAGAACT
    GCAGTGTTGC AAACCTTGAG TCCCCTCCAC ACAGATGACA GCACACATGA AGCGGTAAAA
    GTAGACTCTA CAAGCTGTGA ATTTTCACAT ATGGTCAGTT TGCTTACACA CAAACATCCG
    AATCTTGTCC AAGAAGACTT CGTATTACCA CTGACTGGAG CTCATAAGGC TGATGATACT
    CATGTATTAG AAAACATGGA GAGAAAGCAT GAAACTTTCC CAATAAATGC TCTTAAAAGC
    ACTGAATCAA TATGTGAAAC TTCTGATCCT CTTTTTAAGC AAACATTGCA ATTGCACAAC
    TCGGAGGTTG AGGGCGCATT TGTTTCATGT TGTCATATTT TGCCCATCAC CAACTTAGAG
    GCCATTTTAG AGACGGACCG TTCCCCGGAG AGCTCATTTG CAGTAGAAAA TGAACTGGAT
    GAGCAACAGA GAGGAAGTCC TTTATCAGAA GGTGGATTAA ATGAGGACCT AAGCTCTTGT
    GAGGATGTCA AAAACCGCCC ACATGACAGC AGTTGTGATA TGCATACAAA AGCCAATGTA
    GCCAATTTCA TGAATGAACC CGATGCTGGA GAAACAATGA ACTCTTTATC AAATGGGTTT
    GACAGCAGTG ATGACCATAG TGTTTCCAGT TTGGTGTCAC AAGACCTTGA CTCCAGTACC
    AACAGTGAGG AGACGGAAGA CACCATGCAG CCATCGTTTG GTCTTCGTGG TAATGCGAGA
    AATAAAAGAA CACAGTCAGC CAAGGGCTCT ATGTTTTCTG GTTTGATTCG AATGCCCACC
    TTCCGCAAGC CAAAACGAGA ACTCAAACGC AATAAAAACA GGGTTGATCC GAAAGTTAGG
    GCATCCAGTG GGGATGGAGA TGAACAAGAT GAGATGAAGA ACAACAGGTC ACCCACATGC
    AGCAAATCTC ACTCTTCACT GTACAAGACC CCTATATCTC AAAGTGCAGA TCACCTTACT
    GATATTACAA AGCATTCTGA CCACTCAAAT GATGATGTTT TTGAAAGGGC TTTTGCTTTA
    AATGTGCAAA ATAGAGAGAA ACACATGCAA CCTCAACACC CGTTTGAGCA GCAGCAGCAG
    AAAGATGGAG ATGCACTCAG TTTTAGAGCT GGCCAGACAA CAGAGGGGTT GCAACAAAAA
    AGATGCAAGA GCATTGATAA CTTAAACTTA CGCATGAAGC TGGCATTGGC TCACAAGTCT
    ATTTCTAGCC TGTTTGAGAC CAGATATCCT GAGAGGGACC AAGAGCAAAT CTTACAGTTA
    GAGAATGAGG ATGGGAAAAC CAAGCAATCA TGGAGAAAGA CCAAACGATG TAACGAAGCA
    GAACTTTGCA AAAGAACTCC TGTTCCTGGG ACGGTCTGTG ACAAGTGCAT ACATCAGAGC
    CAAATTGATT GTGCCTTCTG TGCTTATCAG AACCGGTCCA TATTAACCAA CTTACCAAAT
    GTCCAAGCAA GATTGAAGAA CACGTGCCAT TCTGACCCGC AGAGTTTGAA ATCTGCACCT
    CAGGAAGGGT TTGAAGATGG TAAATATCTG GAGGAAGGTG AGCAGCCTTG TGGTGCTTTC
    TCAGATTCAG ACCAAGCATC GGTAGATGGT TTTGAGTCCA GTGTAGAGGA CAGGTCGCTG
    TCACCTGCAC ACCCCACTGT TCTTGCACTT TCAAACCAAA TGTCACCTAC CTGGACAAGG
    TCCCTAAGCT GCTTCGAGAC AACTGATACA CCGACAAGGC CCATGAGCCC CAAACCACAC
    AGTCCTGGTC TGGGCTGGAC ACAGCGGAGG AGCTTCCGCT ACCCCTCAAG ATCTGTTGCA
    TCTTCTCTCT GCTTGCTTGG TCAGGGAGTG AGCACTGACG GTCTCTTAGA CCCACCTCAG
    AGGCCGACAT CTCTAAAACC AAGGACAACA CAACTCACCA CAGCTCACTC CTTCGATTCT
    GAGTACTTAC TGGAGGACAG CAGTTCAGAC AACCAGTCTC AGAATAGCCT GAATTCAGCC
    TCCCTGATCA GCAAAACAGA GCATGTACAG CACATTACTG AAGCAGAGAG AAGACCACAA
    GAAAATGCAT GTGTGCTTCG GGTGAAGTCC AGAGATCCAG GTACAGCTGC GCCCAGGCCT
    ATGTCAGACC TGTACGGCTG GACCATGTCT CTTCAGGGCA CCAGAGAGTT GGCGCTCGAC
    TTGGAGAGGA AGACTAACGC ACATCAGCGC TCATGCTCAT ATGAAACATT GACCGATGTG
    GATAACATCC GCAAAAAGAA GCTAAGCAAG CAGCAAAGTT TGCGGCAGAT TAGCACATCA
    ACATCTGAGA GGCATGAGAA ATTGCGAGCT AGACTTTCCC TCACATCCCC GGAGCAGTTC
    CCCTCTGTTC CTCTCAAAGA CCACTTCTTC TCTCAGAGCA CACCTGTTGG GCTGGACTAT
    CTGGGCTGGG CTCACCGTGC ATCCTTTTCA GAAACGAACC TCAGCGCACC GGAAAAAGAG
    GCACATGGAC AGGTCGCACC TCTCGCATTG GTGATAACTG ATGGGCCACA GGATAAGTCT
    GGTCTTGCAG ATGAGGTGGG CAGTGAAGAT GATCTGTACA ATGAATTCCG CAGCTCCTCC
    AACCGATTCG GACACCCAGG AGGAGGAGGA GAACAGCTGG CCATAAATGA GCTGATCAGT
    GATGGCAGTG TGTGCGCGGA GGCTCTATGG GACCACGTCA CCATGGATGA CCAGGAGCTG
    GGCTTTAAAG CTGGAGATGT CATTGAAGTA GTGGACGCTA CGAACAAGGA GTGGTGGTGG
    GGCAGGGTGC TGGACAGCGA GGGCTGGTTC CCCGCCAGCT TTGTTCGGTT ACGGGTGAAC
    CAAGATGAGC CCATGGAGGA ATACCTAGCC CATCTGGACG GGGCCTCAGA GGGGTGTGGG
    GCCAGCATGG GGGGACCCTT GGGACCTGGA CTGCCATGCA AGGAGCAGAT GAGAGCCAAC
    GTCATCAATG AGATCATGAG CACAGAGAGA GATTACATTA AACACCTGAA GGACATATGT
    GAGGGCTATA TAAAACAATG TCGGAAGAGG ACAGATATGT TTACAGAAGA GCAACTGCGC
    ACTATCTTTG GCAACATCGA CGAGCTCTAC CGATTCCAGA AGAAGTTCCT CAAAGCCTTG
    GAGAAAAAAT TCAACAAAGA GCATCCTCAC CTCAGCGAGA TCGGCTCCTG CTTCTTAGAG
    CAGCAAACAA ACTTCCAGAT CTATTCTGAG TATTGCAACA ACCACCCCAA TGCCTGCGTG
    CAGCTCTCCA AACTGATGAA GATCAAGAAG TATGTGTTCT TCTTTGAGGC TTGTCGCCTG
    CTCCAGAAGA TGATTGACAT TTCGTTGGAT GGATTCCTAC TTACTCCTGT TCAGAAGATC
    TGCAAGTATC CTCTGCAGCT CGCTGAACTG CTCAAGTACA CCAACCCACA GCACAGAGAT
    TATAAGGATG TGGAAGCTGC CTTAAACGCT ATGAAGAATG TGGCCAGACT GATCAATGAG
    AGAAAGAGGC GTCTAGAAAA TATTGACAAA ATCGCTCAGT GGCAGAGCTC CATAGAAGAC
    TGGGAGGGTG AAGACGTCTT GAGCAGGAGC TCTGATCTGA TCTTTTCTGG AGACCTGACC
    AAGATCTCCC AGCCACAGGC TAAAGGCCAG CAGAGAATGT TCTTCCTCTT TGACCACCAA
    CTGGTTTTCT GTAAGAAGGA TCTGCTTCGG AGGGATATCT TGTACTACAA GGGTCGGTTA
    GACATGGATG AGATGGAGGT GGTGGATGTG GAGGATGGGA AGGATAAGGA CTTTAATGTG
    AGTGTGAAGA ATGCCTTGAA ATTGTGTTCT CCTGGAGGAG AGGAGGTTCA TCTCCTGTGT
    GCCAAGAAGC CCGAACAAAA GCAGCGCTGG CTGAGAGCCT TCACAGATGA GCGGGAGCAG
    GTCCAGCATG ACCTCGAGAC AGGTTTTACG ATTACAGAGG TCCAGAAGAA GCAGGCAATG
    TTGAATGCAA CTAAGAGTCA TCCAACAGGG AAGCCTAAAG CATTAGCTTT ACAGCACCTT
    TTGGCACTCA GAGCTCCACT CCTTCACCCC AAAACCAAGT GCCTGCGTTT GTCCGAGCCT
    CCAGGACCTC TGGTTCCCAG GGCCACCCTA ACTTCACCTC TCCTCCCTGA AGGGCCTCAG
    CGCAGGCAGG GGCGGGGGGT TATGGTGCCC CCTGGGGTCC TGCGCAGGGC TATATCCTGG
    GTCTCCTCTC AAAGTAGAGA GCCCATCGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322706.1
    CDS347..9559
    Translation

    Target ORF information:

    RefSeq Version XM_021467031.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA ACCACAGATA
    TCAGTAGAGC ACAGCCAACC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAT
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAT TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA TCCACAGATA TCAGTAGAGC ACAGCCGAAC ACATATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAACC AACCACAGAT GTCAGTAGAG
    CACAACCAAC CGCAGATGTC AGTAGACCAC AGCCAACCAC AGATATCAGT AGACCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGCAGAGCA CAGGCATTCA
    CAGATGTCAG TAGAGCACAG CCAAGCACAG ATGTCAGTAG ACCACAGTCA ACCACAGATG
    TCAGTAGGCC ACAGTCAATC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATGTC AGCAGAGCAC
    AGGCAATCAC AGATGTCAGT AGAGCACAGC CAACCTCAGA TGTTAGTAGA GCACAGCCAA
    TCACAGATGT CAGTAGAGCA CAGCCAATCA CAGATGTCAG TAGAGCACAG CCAATCGCAG
    ATGTCAGTAG AGCACAGCCA ACCACAGATG TTAGTAGAGC ACAGCCAACC ACAGTTATTT
    GTAGAGCACA GCCAATCACA GAACTCTGCT GATCACAGCC AGTCACAAGC TTCTTTAAAC
    TGCAGCCAAC CACAACTCTC AGAAGAATTC AGGCCATCAC AAGATTTTGT TGAACACAAC
    CAATCAAATA AAACTGTAGA TCTCAACCAG TCACAGGATT CAGTAAAACA CAGTCAGTTG
    CAGGATACAA TGGAATACAG TAAAACACAA GCCTCAGTAG AGGAGGTTCC AACCAAACAC
    AGCCAGACAG AGAATTTAGT AGAAGACAGG CAATCACAGT ATTCAATAGA ACACAGAAAA
    TCACAGACAT TAGTAGAAGA CAGTCCAACA CATGATTTTG TTGAACTTTG TCAATCAGGG
    CACTTAGCAG AACACAGCCA GTCAGATTGT TTGATAGAAT GCAGTCAATC TACGAATTCA
    GTTAAACACA GCCAATCACA GGACACAGCA GAGCACAACC AATCAGAAGC TTCTGTAATC
    TGCAGTCAAT CAAAGAATTC AATAGAACAC GGTCCATCAC AGGCCTCAGT AGAGAGGTCA
    TCGCAAGATT TTGTTGAAGA CTCACTAGAA CACAGTCAGT CACATGATAT CGCAGAGTGT
    ATTCTGTCGC AAAATGTAAT GGAACACAGC AAATCACAAG TCTTAGATGA TGACAGGCCA
    TCACAAGTTT ATGTTGGAGA CAACCAATCA GATAAATCTG TAGAAGTCAA CATTTCACAA
    GATTCAGAAG AAGACAGGTC ATTAACAGAT TTTGTAGTAC ACAGCCAATC AGAGAACTCA
    GTAGAACACA GCCAGTCACA GGATTTAGTA GAACCTAGGC AATCACATGA TTTAATAGAA
    CACTTCAAAT CACTAGACAA CACAGAGCAC AAGCAATCAG ACATGAGTTA TATAAACTGC
    AGTCAATCAC AGGCCTCAGT AGAAATCTTG CAATCACACG AGTCTGTTGA ACACAGCCAA
    TCAGAACAGT CAGTAAAACA CAGCCAGTCA CAAGACACTG ACAATTTTAG CCAGACACAG
    GATTTAATTA AGCACAGCCA ATCACAGACT TTCATAAAAT GCAAACACAA CCAATCACTG
    GATGGCGTAC AATATGATCA GTCAAAGATC TTAATAGAAC AAACCCAATC ACAGGATTCC
    GTAAAGGACA ATCCATCCCA AGATCTTGTC AAACATAGCC AATCACAGGA CACAGTAGAA
    CCGAGTCAAT CACAGGTCTC AGTAGAACAC ATCCAATCAC AGGATTCCCA AGACTGCAGC
    CATTCACAGA ACTCAACAGT ACACAGCCAA TCACAAACTT CAGTGGGACA AAGCCAATCC
    CAGGATTTGG TAGAACAAAG TCGATCACCA GCTGCAGCCG AAAACAGTCA ATCACAGACT
    TCAGTGGAAC ACAGCCAATC ACTGGATTCA GTGGAACACA TGAAATCTCA GACTTCGGTG
    GAACACAGGC AATCACAGGT TGAATTGATT AAGGCACATT CCATGAACAC CCGGTGGATG
    GTGACTGAGT CAGACACAGT AGCGTTTTAT GACAAGGTGC GATTAAAACA AGACTGCATG
    GATGTTTGCG CAGATTGTGA AGTCGTTGAA TCCTTGGGAG TTTTATCAAA ACATGCTGAC
    AATTTGAACT TCGAAAATAA CGAAAGACTA AATTTCAAAA CACAGGACTT AAATGACACT
    TCACCTGTTG TTTCTGAATA CGAGTGTTTC CCACATGTGC CTAATGCATC TTCTATTGGA
    GTGCCAGATT CACTGCATCA GTGCACGATT AGCAATTCAC CCTCAGACCT GCAGGTTAAA
    ACTCAGCAGA GTTGGCCAGT ATTACAAATA CACACCGAAA AAGACGATTA CTCAGTTAGT
    AGGTATCACA TGGATGGTTA CTGTTTGCAG GACAGTGAGC AAACAGAGTT TTACAAAACC
    TACCAGTGCC CTAACAGTAC CATTAATTTT AATACACAGG GTGAGTTCAC TGAAGCTCGG
    AAGGTTGTAT CAACTGAAGG TGTATTTACT AGAGGTTGTA TCAACACCAA ACTTTTTGAT
    GGCAAAAGTG TTGACATTGT AGGAGGACCG CAGAAGGACA GCAACAAGCA TCACGTTATG
    AAAGGGAAAG AATGTACAAT AGTTCCTTCA ATAGAGCAGT CAGTAATTTA CTCTGACAGT
    TCAGGATGCA TAGAATATAA AAGGCCCAAA ACGTTGAGCA TTGCTTGTCC TGACATGACG
    ACTGCTGAAT CTGAAGGGTG TCCTGTAGAC AGTCCTGTTG AATCACTTTC TACACTGTTT
    GAGATTCCAC ATGACAGATC TACAGTCAGC CCCACAGACA TTTGCTTTGA GTTTAGTGAT
    GGCCCTCATA TGAACTGTAC ACAAGTAGCA CAGAGTGTTA TAGAATATTC AGATGCCCCC
    AAACTATTTC ACCAGTTTGA CACCCTGTCT GACAAACAAA GAAGACCTAA TGAGTCAGCT
    GAAGCAATTG CGTCGAATGT TGATATTTTG TCTGGTGCAC GTTCCGATCC TTTCACAAAT
    GCACAGACTC CTTTTGATTA TTCCCAGGTG AACAGCAGGA ACTATACACA GAGCTCAGAC
    ACAAATAATA ACATTAAAGC CATTACTTTA GAAGAGTGTG CCTCGGGAGT ACAAATGTCA
    TTGCCTAGTT CATTGCAGAA ATACAGTAGG TTTACAAATA ATGCTGAATG CTTAGTCTCA
    AAAAGGGGTG ATTACAAAAA CATTGATTCT TTTAGTGCTC GTAATACTCT GGCTACCCCC
    TTCAAAGCAC CTGAAATGAA GACTGAAGTG AACAGCAGAG AAAATGTTGT GAAATCTAGC
    AGTCACATAG ATGTTACACA GAGGAAGCTC TGTGATTGCT GCGGTGGAAA GGAGTGCCAG
    CTGCAGACAG CGGGTGTATT TCAGTTCAAG GGCACAGACG CCATCAGTCA GCGCAGAACT
    GCAGTGTTGC AAACCTTGAG TCCCCTCCAC ACAGATGACA GCACACATGA AGCGGTAAAA
    GTAGACTCTA CAAGCTGTGA ATTTTCACAT ATGGTCAGTT TGCTTACACA CAAACATCCG
    AATCTTGTCC AAGAAGACTT CGTATTACCA CTGACTGGAG CTCATAAGGC TGATGATACT
    CATGTATTAG AAAACATGGA GAGAAAGCAT GAAACTTTCC CAATAAATGC TCTTAAAAGC
    ACTGAATCAA TATGTGAAAC TTCTGATCCT CTTTTTAAGC AAACATTGCA ATTGCACAAC
    TCGGAGGTTG AGGGCGCATT TGTTTCATGT TGTCATATTT TGCCCATCAC CAACTTAGAG
    GCCATTTTAG AGACGGACCG TTCCCCGGAG AGCTCATTTG CAGTAGAAAA TGAACTGGAT
    GAGCAACAGA GAGGAAGTCC TTTATCAGAA GGTGGATTAA ATGAGGACCT AAGCTCTTGT
    GAGGATGTCA AAAACCGCCC ACATGACAGC AGTTGTGATA TGCATACAAA AGCCAATGTA
    GCCAATTTCA TGAATGAACC CGATGCTGGA GAAACAATGA ACTCTTTATC AAATGGGTTT
    GACAGCAGTG ATGACCATAG TGTTTCCAGT TTGGTGTCAC AAGACCTTGA CTCCAGTACC
    AACAGTGAGG AGACGGAAGA CACCATGCAG CCATCGTTTG GTCTTCGTGG TAATGCGAGA
    AATAAAAGAA CACAGTCAGC CAAGGGCTCT ATGTTTTCTG GTTTGATTCG AATGCCCACC
    TTCCGCAAGC CAAAACGAGA ACTCAAACGC AATAAAAACA GGGTTGATCC GAAAGTTAGG
    GCATCCAGTG GGGATGGAGA TGAACAAGAT GAGATGAAGA ACAACAGGTC ACCCACATGC
    AGCAAATCTC ACTCTTCACT GTACAAGACC CCTATATCTC AAAGTGCAGA TCACCTTACT
    GATATTACAA AGCATTCTGA CCACTCAAAT GATGATGTTT TTGAAAGGGC TTTTGCTTTA
    AATGTGCAAA ATAGAGAGAA ACACATGCAA CCTCAACACC CGTTTGAGCA GCAGCAGCAG
    AAAGATGGAG ATGCACTCAG TTTTAGAGCT GGCCAGACAA CAGAGGGGTT GCAACAAAAA
    AGATGCAAGA GCATTGATAA CTTAAACTTA CGCATGAAGC TGGCATTGGC TCACAAGTCT
    ATTTCTAGCC TGTTTGAGAC CAGATATCCT GAGAGGGACC AAGAGCAAAT CTTACAGTTA
    GAGAATGAGG ATGGGAAAAC CAAGCAATCA TGGAGAAAGA CCAAACGATG TAACGAAGCA
    GAACTTTGCA AAAGAACTCC TGTTCCTGGG ACGGTCTGTG ACAAGTGCAT ACATCAGAGC
    CAAATTGATT GTGCCTTCTG TGCTTATCAG AACCGGTCCA TATTAACCAA CTTACCAAAT
    GTCCAAGCAA GATTGAAGAA CACGTGCCAT TCTGACCCGC AGAGTTTGAA ATCTGCACCT
    CAGGAAGGGT TTGAAGATGG TAAATATCTG GAGGAAGGTG AGCAGCCTTG TGGTGCTTTC
    TCAGATTCAG ACCAAGCATC GGTAGATGGT TTTGAGTCCA GTGTAGAGGA CAGGTCGCTG
    TCACCTGCAC ACCCCACTGT TCTTGCACTT TCAAACCAAA TGTCACCTAC CTGGACAAGG
    TCCCTAAGCT GCTTCGAGAC AACTGATACA CCGACAAGGC CCATGAGCCC CAAACCACAC
    AGTCCTGGTC TGGGCTGGAC ACAGCGGAGG AGCTTCCGCT ACCCCTCAAG ATCTGTTGCA
    TCTTCTCTCT GCTTGCTTGG TCAGGGAGTG AGCACTGACG GTCTCTTAGA CCCACCTCAG
    AGGCCGACAT CTCTAAAACC AAGGACAACA CAACTCACCA CAGCTCACTC CTTCGATTCT
    GAGTACTTAC TGGAGGACAG CAGTTCAGAC AACCAGTCTC AGAATAGCCT GAATTCAGCC
    TCCCTGATCA GCAAAACAGA GCATGTACAG CACATTACTG AAGCAGAGAG AAGACCACAA
    GAAAATGCAT GTGTGCTTCG GGTGAAGTCC AGAGATCCAG GTACAGCTGC GCCCAGGCCT
    ATGTCAGACC TGTACGGCTG GACCATGTCT CTTCAGGGCA CCAGAGAGTT GGCGCTCGAC
    TTGGAGAGGA AGACTAACGC ACATCAGCGC TCATGCTCAT ATGAAACATT GACCGATGTG
    GATAACATCC GCAAAAAGAA GCTAAGCAAG CAGCAAAGTT TGCGGCAGAT TAGCACATCA
    ACATCTGAGA GGCATGAGAA ATTGCGAGCT AGACTTTCCC TCACATCCCC GGAGCAGTTC
    CCCTCTGTTC CTCTCAAAGA CCACTTCTTC TCTCAGAGCA CACCTGTTGG GCTGGACTAT
    CTGGGCTGGG CTCACCGTGC ATCCTTTTCA GAAACGAACC TCAGCGCACC GGAAAAAGAG
    GCACATGGAC AGGTCGCACC TCTCGCATTG GTGATAACTG ATGGGCCACA GGATAAGTCT
    GGTCTTGCAG ATGAGGTGGG CAGTGAAGAT GATCTGTACA ATGAATTCCG CAGCTCCTCC
    AACCGATTCG GACACCCAGG AGGAGGAGGA GAACAGCTGG CCATAAATGA GCTGATCAGT
    GATGGCAGTG TGTGCGCGGA GGCTCTATGG GACCACGTCA CCATGGATGA CCAGGAGCTG
    GGCTTTAAAG CTGGAGATGT CATTGAAGTA GTGGACGCTA CGAACAAGGA GTGGTGGTGG
    GGCAGGGTGC TGGACAGCGA GGGCTGGTTC CCCGCCAGCT TTGTTCGGTT ACGGGTGAAC
    CAAGATGAGC CCATGGAGGA ATACCTAGCC CATCTGGACG GGGCCTCAGA GGGGTGTGGG
    GCCAGCATGG GGGGACCCTT GGGACCTGGA CTGCCATGCA AGGAGCAGAT GAGAGCCAAC
    GTCATCAATG AGATCATGAG CACAGAGAGA GATTACATTA AACACCTGAA GGACATATGT
    GAGGGCTATA TAAAACAATG TCGGAAGAGG ACAGATATGT TTACAGAAGA GCAACTGCGC
    ACTATCTTTG GCAACATCGA CGAGCTCTAC CGATTCCAGA AGAAGTTCCT CAAAGCCTTG
    GAGAAAAAAT TCAACAAAGA GCATCCTCAC CTCAGCGAGA TCGGCTCCTG CTTCTTAGAG
    CAGCAAACAA ACTTCCAGAT CTATTCTGAG TATTGCAACA ACCACCCCAA TGCCTGCGTG
    CAGCTCTCCA AACTGATGAA GATCAAGAAG TATGTGTTCT TCTTTGAGGC TTGTCGCCTG
    CTCCAGAAGA TGATTGACAT TTCGTTGGAT GGATTCCTAC TTACTCCTGT TCAGAAGATC
    TGCAAGTATC CTCTGCAGCT CGCTGAACTG CTCAAGTACA CCAACCCACA GCACAGAGAT
    TATAAGGATG TGGAAGCTGC CTTAAACGCT ATGAAGAATG TGGCCAGACT GATCAATGAG
    AGAAAGAGGC GTCTAGAAAA TATTGACAAA ATCGCTCAGT GGCAGAGCTC CATAGAAGAC
    TGGGAGGGTG AAGACGTCTT GAGCAGGAGC TCTGATCTGA TCTTTTCTGG AGACCTGACC
    AAGATCTCCC AGCCACAGGC TAAAGGCCAG CAGAGAATGT TCTTCCTCTT TGACCACCAA
    CTGGTTTTCT GTAAGAAGGA TCTGCTTCGG AGGGATATCT TGTACTACAA GGGTCGGTTA
    GACATGGATG AGATGGAGGT GGTGGATGTG GAGGATGGGA AGGATAAGGA CTTTAATGTG
    AGTGTGAAGA ATGCCTTGAA ATTGTGTTCT CCTGGAGGAG AGGAGGTTCA TCTCCTGTGT
    GCCAAGAAGC CCGAACAAAA GCAGCGCTGG CTGAGAGCCT TCACAGATGA GCGGGAGCAG
    GTCCAGCATG ACCTCGAGAC AGGTTTTACG ATTACAGAGG TCCAGAAGAA GCAGGCAATG
    TTGAATGCAA CTAAGAGTCA TCCAACAGGG AAGCCTAAAG CATTAGCTTT ACAGCACCTT
    TTGGCACTCA GAGCTCCACT CCTTCACCCC AAAACCAAGT GCCTGCGTTT GTCCGAGCCT
    CCAGGACCTC TGGTTCCCAG GGCCACCCTA ACTTCACCTC TCCTCCCTGA AGGGCCTCAG
    CGCAGGCAGG GGCGGGGGGT TATGGTGCCC CCTGGGGTCC TGCGCAGGGC TATATCCTGG
    GTCTCCTCTC AAAGTAGAGA GCCCATCGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42946
    Clone ID Related Accession (Same CDS sequence) XM_009298437.2 , XM_009298437.3
    Accession Version XM_009298437.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296712.1
    CDS372..9638
    Translation

    Target ORF information:

    RefSeq Version XM_009298437.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298437.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42946
    Clone ID Related Accession (Same CDS sequence) XM_009298437.2 , XM_009298437.3
    Accession Version XM_009298437.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298437.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
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    301
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    481
    541
    601
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2641
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    4141
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    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296712.1
    CDS347..9613
    Translation

    Target ORF information:

    RefSeq Version XM_009298437.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298437.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42947
    Clone ID Related Accession (Same CDS sequence) XM_009298440.2 , XM_009298440.3
    Accession Version XM_009298440.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298440.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCTGTGA CCCGGCCCTA CTATGACTTC
    CTGCTGCGGC AGAAACACCC GACGCTGCCC ACCGCCCTGC CCCAGCAGCA GGTCATCATG
    CTGGCCGAAC CCAAACGCAA GACCTCCAAT TTCTGGCACA ACATCGGACG CTTGACACCG
    TTCAAAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296715.1
    CDS369..9560
    Translation

    Target ORF information:

    RefSeq Version XM_009298440.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298440.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCTGTGA CCCGGCCCTA CTATGACTTC
    CTGCTGCGGC AGAAACACCC GACGCTGCCC ACCGCCCTGC CCCAGCAGCA GGTCATCATG
    CTGGCCGAAC CCAAACGCAA GACCTCCAAT TTCTGGCACA ACATCGGACG CTTGACACCG
    TTCAAAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42947
    Clone ID Related Accession (Same CDS sequence) XM_009298440.2 , XM_009298440.3
    Accession Version XM_009298440.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCTGTGA CCCGGCCCTA CTATGACTTC
    CTGCTGCGGC AGAAACACCC GACGCTGCCC ACCGCCCTGC CCCAGCAGCA GGTCATCATG
    CTGGCCGAAC CCAAACGCAA GACCTCCAAT TTCTGGCACA ACATCGGACG CTTGACACCG
    TTCAAAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296715.1
    CDS342..9533
    Translation

    Target ORF information:

    RefSeq Version XM_009298440.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298440.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCTGTGA CCCGGCCCTA CTATGACTTC
    CTGCTGCGGC AGAAACACCC GACGCTGCCC ACCGCCCTGC CCCAGCAGCA GGTCATCATG
    CTGGCCGAAC CCAAACGCAA GACCTCCAAT TTCTGGCACA ACATCGGACG CTTGACACCG
    TTCAAAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42948
    Clone ID Related Accession (Same CDS sequence) XM_009298441.3 , XM_009298441.2
    Accession Version XM_009298441.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGCCAAACA GAGGCTGTGA CCACAGAGAC ATGGCACAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296716.1
    CDS242..9337
    Translation

    Target ORF information:

    RefSeq Version XM_009298441.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298441.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGCCAAACA GAGGCTGTGA CCACAGAGAC ATGGCACAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42948
    Clone ID Related Accession (Same CDS sequence) XM_009298441.3 , XM_009298441.2
    Accession Version XM_009298441.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGCCAAACA GAGGCTGTGA CCACAGAGAC ATGGCACAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296716.1
    CDS268..9363
    Translation

    Target ORF information:

    RefSeq Version XM_009298441.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298441.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGCCAAACA GAGGCTGTGA CCACAGAGAC ATGGCACAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42949
    Clone ID Related Accession (Same CDS sequence) XM_009298442.2 , XM_009298442.3
    Accession Version XM_009298442.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTTGAGTG CTATATACTT CCTACGAAGC TTCTTTAAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296717.1
    CDS163..9258
    Translation

    Target ORF information:

    RefSeq Version XM_009298442.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298442.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTTGAGTG CTATATACTT CCTACGAAGC TTCTTTAAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42949
    Clone ID Related Accession (Same CDS sequence) XM_009298442.2 , XM_009298442.3
    Accession Version XM_009298442.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTTGAGTG CTATATACTT CCTACGAAGC TTCTTTAAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296717.1
    CDS160..9255
    Translation

    Target ORF information:

    RefSeq Version XM_009298442.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298442.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTTGAGTG CTATATACTT CCTACGAAGC TTCTTTAAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42950
    Clone ID Related Accession (Same CDS sequence) XM_009298443.2 , XM_009298443.3
    Accession Version XM_009298443.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8967bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298443.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
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    301
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    541
    601
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
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    4141
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    8341
    8401
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    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGTTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296718.1
    CDS372..9338
    Translation

    Target ORF information:

    RefSeq Version XM_009298443.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298443.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGTTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42950
    Clone ID Related Accession (Same CDS sequence) XM_009298443.2 , XM_009298443.3
    Accession Version XM_009298443.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8967bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298443.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGTTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296718.1
    CDS347..9313
    Translation

    Target ORF information:

    RefSeq Version XM_009298443.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298443.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG
    ATCAGCAAAA CAGAGTTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.