ORF » Species Summary » Danio rerio » arhgef4 cDNA ORF clone

arhgef4 cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following arhgef4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa06855 XM_682312.8
Latest version!		 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa150441 XM_021467029.1
Latest version!		 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa150442 XM_021467031.1
Latest version!		 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42946 XM_009298437.3
Latest version!		 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42947 XM_009298440.3
Latest version!		 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42948 XM_009298441.3
Latest version!		 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42949 XM_009298442.3
Latest version!		 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42950 XM_009298443.3
Latest version!		 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa42948 XM_009298441.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42947 XM_009298440.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa06855 XM_682312.7 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa42949 XM_009298442.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42946 XM_009298437.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42950 XM_009298443.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    arhgef4 ( XM_682312.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_687404.3 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa06855
    Clone ID Related Accession (Same CDS sequence) XM_682312.7 , XM_682312.8
    Accession Version XM_682312.7 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_682312.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCGCACCC AGCACAGTGC CGGTGAGGTG GGCGTCCCGC TGGCAGGAGG AGGCTGCAGT 
    GGTGGCAGCT CAGAGCAGTC GCCTGGCTCC GACTTGGACT GTAGCCCCGC TCTGGGACTG 
    GCCGGAGGAG TTCGAGCGAT AAGAGGGGTC ATGTCCCATT ACTGGAGCCT AGAGAGCCTG 
    CACTCCACCA ACGCATTGGT GATAACTGAT GGGCCACAGG ATAAGTCTGG TCTTGCAGAT 
    GAGGTGGGCA GTGAAGATGA TCTGTACAAT GAATTCCGCA GCTCCTCCAA CCGATTCGGA 
    CACCCAGGAG GAGGAGGAGA ACAGCTGGCC ATAAATGAGC TGATCAGTGA TGGCAGTGTG 
    TGCGCGGAGG CTCTATGGGA CCACGTCACC ATGGATGACC AGGAGCTGGG CTTTAAAGCT 
    GGAGATGTCA TTGAAGTAGT GGACGCTACG AACAAGGAGT GGTGGTGGGG CAGGGTGCTG 
    GACAGCGAGG GCTGGTTCCC CGCCAGCTTT GTTCGGTTAC GGGTGAACCA AGATGAGCCC 
    ATGGAGGAAT ACCTAGCCCA TCTGGACGGG GCCTCAGAGG GGTGTGGGGC CAGCATGGGG 
    GGACCCTTGG GACCTGGACT GCCATGCAAG GAGCAGATGA GAGCCAACGT CATCAATGAG 
    ATCATGAGCA CAGAGAGAGA TTACATTAAA CACCTGAAGG ACATATGTGA GGGCTATATA 
    AAACAATGTC GGAAGAGGAC AGATATGTTT ACAGAAGAGC AACTGCGCAC TATCTTTGGC 
    AACATCGACG AGCTCTACCG ATTCCAGAAG AAGTTCCTCA AAGCCTTGGA GAAAAAATTC 
    AACAAAGAGC ATCCTCACCT CAGCGAGATC GGCTCCTGCT TCTTAGAGCA GCAAACAAAC 
    TTCCAGATCT ATTCTGAGTA TTGCAACAAC CACCCCAATG CCTGCGTGCA GCTCTCCAAA 
    CTGATGAAGA TCAAGAAGTA TGTGTTCTTC TTTGAGGCTT GTCGCCTGCT CCAGAAGATG 
    ATTGACATTT CGTTGGATGG ATTCCTACTT ACTCCTGTTC AGAAGATCTG CAAGTATCCT 
    CTGCAGCTCG CTGAACTGCT CAAGTACACC AACCCACAGC ACAGAGATTA TAAGGATGTG 
    GAAGCTGCCT TAAACGCTAT GAAGAATGTG GCCAGACTGA TCAATGAGAG AAAGAGGCGT 
    CTAGAAAATA TTGACAAAAT CGCTCAGTGG CAGAGCTCCA TAGAAGACTG GGAGGGTGAA 
    GACGTCTTGA GCAGGAGCTC TGATCTGATC TTTTCTGGAG ACCTGACCAA GATCTCCCAG 
    CCACAGGCTA AAGGCCAGCA GAGAATGTTC TTCCTCTTTG ACCACCAACT GGTTTTCTGT 
    AAGAAGGATC TGCTTCGGAG GGATATCTTG TACTACAAGG GTCGGTTAGA CATGGATGAG 
    ATGGAGGTGG TGGATGTGGA GGATGGGAAG GATAAGGACT TTAATGTGAG TGTGAAGAAT 
    GCCTTGAAAT TGTGTTCTCC TGGAGGAGAG GAGGTTCATC TCCTGTGTGC CAAGAAGCCC 
    GAACAAAAGC AGCGCTGGCT GAGAGCCTTC ACAGATGAGC GGGAGCAGGT CCAGCATGAC 
    CTCGAGACAG GTTTTACGAT TACAGAGGTC CAGAAGAAGC AGGCAATGTT GAATGCAACT 
    AAGAGTCATC CAACAGGGAA GCCTAAAGCA TTAGCTTTAC AGCACCTTTT GGCACTCAGA 
    GCTCCACTCC TTCACCCCAA AACCAAGTGC CTGCGTTTGT CCGAGCCTCC AGGACCTCTG 
    GTTCCCAGGG CCACCCTAAC TTCACCTCTC CTCCCTGAAG GGCCTCAGCG CAGGCAGGGG 
    CGGGGGGTTA TGGTGCCCCC TGGGGTCCTG CGCAGGGCTA TATCCTGGGT CTCCTCTCAA 
    AGTAGAGAGC CCATCGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_687404.3
    CDS238..2178
    Translation

    Target ORF information:

    RefSeq Version XM_682312.7
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_682312.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCGCACCC AGCACAGTGC CGGTGAGGTG GGCGTCCCGC TGGCAGGAGG AGGCTGCAGT 
    GGTGGCAGCT CAGAGCAGTC GCCTGGCTCC GACTTGGACT GTAGCCCCGC TCTGGGACTG 
    GCCGGAGGAG TTCGAGCGAT AAGAGGGGTC ATGTCCCATT ACTGGAGCCT AGAGAGCCTG 
    CACTCCACCA ACGCATTGGT GATAACTGAT GGGCCACAGG ATAAGTCTGG TCTTGCAGAT 
    GAGGTGGGCA GTGAAGATGA TCTGTACAAT GAATTCCGCA GCTCCTCCAA CCGATTCGGA 
    CACCCAGGAG GAGGAGGAGA ACAGCTGGCC ATAAATGAGC TGATCAGTGA TGGCAGTGTG 
    TGCGCGGAGG CTCTATGGGA CCACGTCACC ATGGATGACC AGGAGCTGGG CTTTAAAGCT 
    GGAGATGTCA TTGAAGTAGT GGACGCTACG AACAAGGAGT GGTGGTGGGG CAGGGTGCTG 
    GACAGCGAGG GCTGGTTCCC CGCCAGCTTT GTTCGGTTAC GGGTGAACCA AGATGAGCCC 
    ATGGAGGAAT ACCTAGCCCA TCTGGACGGG GCCTCAGAGG GGTGTGGGGC CAGCATGGGG 
    GGACCCTTGG GACCTGGACT GCCATGCAAG GAGCAGATGA GAGCCAACGT CATCAATGAG 
    ATCATGAGCA CAGAGAGAGA TTACATTAAA CACCTGAAGG ACATATGTGA GGGCTATATA 
    AAACAATGTC GGAAGAGGAC AGATATGTTT ACAGAAGAGC AACTGCGCAC TATCTTTGGC 
    AACATCGACG AGCTCTACCG ATTCCAGAAG AAGTTCCTCA AAGCCTTGGA GAAAAAATTC 
    AACAAAGAGC ATCCTCACCT CAGCGAGATC GGCTCCTGCT TCTTAGAGCA GCAAACAAAC 
    TTCCAGATCT ATTCTGAGTA TTGCAACAAC CACCCCAATG CCTGCGTGCA GCTCTCCAAA 
    CTGATGAAGA TCAAGAAGTA TGTGTTCTTC TTTGAGGCTT GTCGCCTGCT CCAGAAGATG 
    ATTGACATTT CGTTGGATGG ATTCCTACTT ACTCCTGTTC AGAAGATCTG CAAGTATCCT 
    CTGCAGCTCG CTGAACTGCT CAAGTACACC AACCCACAGC ACAGAGATTA TAAGGATGTG 
    GAAGCTGCCT TAAACGCTAT GAAGAATGTG GCCAGACTGA TCAATGAGAG AAAGAGGCGT 
    CTAGAAAATA TTGACAAAAT CGCTCAGTGG CAGAGCTCCA TAGAAGACTG GGAGGGTGAA 
    GACGTCTTGA GCAGGAGCTC TGATCTGATC TTTTCTGGAG ACCTGACCAA GATCTCCCAG 
    CCACAGGCTA AAGGCCAGCA GAGAATGTTC TTCCTCTTTG ACCACCAACT GGTTTTCTGT 
    AAGAAGGATC TGCTTCGGAG GGATATCTTG TACTACAAGG GTCGGTTAGA CATGGATGAG 
    ATGGAGGTGG TGGATGTGGA GGATGGGAAG GATAAGGACT TTAATGTGAG TGTGAAGAAT 
    GCCTTGAAAT TGTGTTCTCC TGGAGGAGAG GAGGTTCATC TCCTGTGTGC CAAGAAGCCC 
    GAACAAAAGC AGCGCTGGCT GAGAGCCTTC ACAGATGAGC GGGAGCAGGT CCAGCATGAC 
    CTCGAGACAG GTTTTACGAT TACAGAGGTC CAGAAGAAGC AGGCAATGTT GAATGCAACT 
    AAGAGTCATC CAACAGGGAA GCCTAAAGCA TTAGCTTTAC AGCACCTTTT GGCACTCAGA 
    GCTCCACTCC TTCACCCCAA AACCAAGTGC CTGCGTTTGT CCGAGCCTCC AGGACCTCTG 
    GTTCCCAGGG CCACCCTAAC TTCACCTCTC CTCCCTGAAG GGCCTCAGCG CAGGCAGGGG 
    CGGGGGGTTA TGGTGCCCCC TGGGGTCCTG CGCAGGGCTA TATCCTGGGT CTCCTCTCAA 
    AGTAGAGAGC CCATCGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_682312.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_687404.3 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID ODa06855
    Clone ID Related Accession (Same CDS sequence) XM_682312.7 , XM_682312.8
    Accession Version XM_682312.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_682312.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCGCACCC AGCACAGTGC CGGTGAGGTG GGCGTCCCGC TGGCAGGAGG AGGCTGCAGT 
    GGTGGCAGCT CAGAGCAGTC GCCTGGCTCC GACTTGGACT GTAGCCCCGC TCTGGGACTG 
    GCCGGAGGAG TTCGAGCGAT AAGAGGGGTC ATGTCCCATT ACTGGAGCCT AGAGAGCCTG 
    CACTCCACCA ACGCATTGGT GATAACTGAT GGGCCACAGG ATAAGTCTGG TCTTGCAGAT 
    GAGGTGGGCA GTGAAGATGA TCTGTACAAT GAATTCCGCA GCTCCTCCAA CCGATTCGGA 
    CACCCAGGAG GAGGAGGAGA ACAGCTGGCC ATAAATGAGC TGATCAGTGA TGGCAGTGTG 
    TGCGCGGAGG CTCTATGGGA CCACGTCACC ATGGATGACC AGGAGCTGGG CTTTAAAGCT 
    GGAGATGTCA TTGAAGTAGT GGACGCTACG AACAAGGAGT GGTGGTGGGG CAGGGTGCTG 
    GACAGCGAGG GCTGGTTCCC CGCCAGCTTT GTTCGGTTAC GGGTGAACCA AGATGAGCCC 
    ATGGAGGAAT ACCTAGCCCA TCTGGACGGG GCCTCAGAGG GGTGTGGGGC CAGCATGGGG 
    GGACCCTTGG GACCTGGACT GCCATGCAAG GAGCAGATGA GAGCCAACGT CATCAATGAG 
    ATCATGAGCA CAGAGAGAGA TTACATTAAA CACCTGAAGG ACATATGTGA GGGCTATATA 
    AAACAATGTC GGAAGAGGAC AGATATGTTT ACAGAAGAGC AACTGCGCAC TATCTTTGGC 
    AACATCGACG AGCTCTACCG ATTCCAGAAG AAGTTCCTCA AAGCCTTGGA GAAAAAATTC 
    AACAAAGAGC ATCCTCACCT CAGCGAGATC GGCTCCTGCT TCTTAGAGCA GCAAACAAAC 
    TTCCAGATCT ATTCTGAGTA TTGCAACAAC CACCCCAATG CCTGCGTGCA GCTCTCCAAA 
    CTGATGAAGA TCAAGAAGTA TGTGTTCTTC TTTGAGGCTT GTCGCCTGCT CCAGAAGATG 
    ATTGACATTT CGTTGGATGG ATTCCTACTT ACTCCTGTTC AGAAGATCTG CAAGTATCCT 
    CTGCAGCTCG CTGAACTGCT CAAGTACACC AACCCACAGC ACAGAGATTA TAAGGATGTG 
    GAAGCTGCCT TAAACGCTAT GAAGAATGTG GCCAGACTGA TCAATGAGAG AAAGAGGCGT 
    CTAGAAAATA TTGACAAAAT CGCTCAGTGG CAGAGCTCCA TAGAAGACTG GGAGGGTGAA 
    GACGTCTTGA GCAGGAGCTC TGATCTGATC TTTTCTGGAG ACCTGACCAA GATCTCCCAG 
    CCACAGGCTA AAGGCCAGCA GAGAATGTTC TTCCTCTTTG ACCACCAACT GGTTTTCTGT 
    AAGAAGGATC TGCTTCGGAG GGATATCTTG TACTACAAGG GTCGGTTAGA CATGGATGAG 
    ATGGAGGTGG TGGATGTGGA GGATGGGAAG GATAAGGACT TTAATGTGAG TGTGAAGAAT 
    GCCTTGAAAT TGTGTTCTCC TGGAGGAGAG GAGGTTCATC TCCTGTGTGC CAAGAAGCCC 
    GAACAAAAGC AGCGCTGGCT GAGAGCCTTC ACAGATGAGC GGGAGCAGGT CCAGCATGAC 
    CTCGAGACAG GTTTTACGAT TACAGAGGTC CAGAAGAAGC AGGCAATGTT GAATGCAACT 
    AAGAGTCATC CAACAGGGAA GCCTAAAGCA TTAGCTTTAC AGCACCTTTT GGCACTCAGA 
    GCTCCACTCC TTCACCCCAA AACCAAGTGC CTGCGTTTGT CCGAGCCTCC AGGACCTCTG 
    GTTCCCAGGG CCACCCTAAC TTCACCTCTC CTCCCTGAAG GGCCTCAGCG CAGGCAGGGG 
    CGGGGGGTTA TGGTGCCCCC TGGGGTCCTG CGCAGGGCTA TATCCTGGGT CTCCTCTCAA 
    AGTAGAGAGC CCATCGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_687404.3
    CDS276..2216
    Translation

    Target ORF information:

    RefSeq Version XM_682312.8
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_682312.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCGCACCC AGCACAGTGC CGGTGAGGTG GGCGTCCCGC TGGCAGGAGG AGGCTGCAGT 
    GGTGGCAGCT CAGAGCAGTC GCCTGGCTCC GACTTGGACT GTAGCCCCGC TCTGGGACTG 
    GCCGGAGGAG TTCGAGCGAT AAGAGGGGTC ATGTCCCATT ACTGGAGCCT AGAGAGCCTG 
    CACTCCACCA ACGCATTGGT GATAACTGAT GGGCCACAGG ATAAGTCTGG TCTTGCAGAT 
    GAGGTGGGCA GTGAAGATGA TCTGTACAAT GAATTCCGCA GCTCCTCCAA CCGATTCGGA 
    CACCCAGGAG GAGGAGGAGA ACAGCTGGCC ATAAATGAGC TGATCAGTGA TGGCAGTGTG 
    TGCGCGGAGG CTCTATGGGA CCACGTCACC ATGGATGACC AGGAGCTGGG CTTTAAAGCT 
    GGAGATGTCA TTGAAGTAGT GGACGCTACG AACAAGGAGT GGTGGTGGGG CAGGGTGCTG 
    GACAGCGAGG GCTGGTTCCC CGCCAGCTTT GTTCGGTTAC GGGTGAACCA AGATGAGCCC 
    ATGGAGGAAT ACCTAGCCCA TCTGGACGGG GCCTCAGAGG GGTGTGGGGC CAGCATGGGG 
    GGACCCTTGG GACCTGGACT GCCATGCAAG GAGCAGATGA GAGCCAACGT CATCAATGAG 
    ATCATGAGCA CAGAGAGAGA TTACATTAAA CACCTGAAGG ACATATGTGA GGGCTATATA 
    AAACAATGTC GGAAGAGGAC AGATATGTTT ACAGAAGAGC AACTGCGCAC TATCTTTGGC 
    AACATCGACG AGCTCTACCG ATTCCAGAAG AAGTTCCTCA AAGCCTTGGA GAAAAAATTC 
    AACAAAGAGC ATCCTCACCT CAGCGAGATC GGCTCCTGCT TCTTAGAGCA GCAAACAAAC 
    TTCCAGATCT ATTCTGAGTA TTGCAACAAC CACCCCAATG CCTGCGTGCA GCTCTCCAAA 
    CTGATGAAGA TCAAGAAGTA TGTGTTCTTC TTTGAGGCTT GTCGCCTGCT CCAGAAGATG 
    ATTGACATTT CGTTGGATGG ATTCCTACTT ACTCCTGTTC AGAAGATCTG CAAGTATCCT 
    CTGCAGCTCG CTGAACTGCT CAAGTACACC AACCCACAGC ACAGAGATTA TAAGGATGTG 
    GAAGCTGCCT TAAACGCTAT GAAGAATGTG GCCAGACTGA TCAATGAGAG AAAGAGGCGT 
    CTAGAAAATA TTGACAAAAT CGCTCAGTGG CAGAGCTCCA TAGAAGACTG GGAGGGTGAA 
    GACGTCTTGA GCAGGAGCTC TGATCTGATC TTTTCTGGAG ACCTGACCAA GATCTCCCAG 
    CCACAGGCTA AAGGCCAGCA GAGAATGTTC TTCCTCTTTG ACCACCAACT GGTTTTCTGT 
    AAGAAGGATC TGCTTCGGAG GGATATCTTG TACTACAAGG GTCGGTTAGA CATGGATGAG 
    ATGGAGGTGG TGGATGTGGA GGATGGGAAG GATAAGGACT TTAATGTGAG TGTGAAGAAT 
    GCCTTGAAAT TGTGTTCTCC TGGAGGAGAG GAGGTTCATC TCCTGTGTGC CAAGAAGCCC 
    GAACAAAAGC AGCGCTGGCT GAGAGCCTTC ACAGATGAGC GGGAGCAGGT CCAGCATGAC 
    CTCGAGACAG GTTTTACGAT TACAGAGGTC CAGAAGAAGC AGGCAATGTT GAATGCAACT 
    AAGAGTCATC CAACAGGGAA GCCTAAAGCA TTAGCTTTAC AGCACCTTTT GGCACTCAGA 
    GCTCCACTCC TTCACCCCAA AACCAAGTGC CTGCGTTTGT CCGAGCCTCC AGGACCTCTG 
    GTTCCCAGGG CCACCCTAAC TTCACCTCTC CTCCCTGAAG GGCCTCAGCG CAGGCAGGGG 
    CGGGGGGTTA TGGTGCCCCC TGGGGTCCTG CGCAGGGCTA TATCCTGGGT CTCCTCTCAA 
    AGTAGAGAGC CCATCGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_021467029.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322704.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa150441
    Clone ID Related Accession (Same CDS sequence) XM_021467029.1
    Accession Version XM_021467029.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAATCACA GAACTCTGCT GATCACAGCC AGTCACAAGC TTCTTTAAAC 
    TGCAGCCAAC CACAACTCTC AGAAGAATTC AGGCCATCAC AAGATTTTGT TGAACACAAC 
    CAATCAAATA AAACTGTAGA TCTCAACCAG TCACAGGATT CAGTAAAACA CAGTCAGTTG 
    CAGGATACAA TGGAATACAG TAAAACACAA GCCTCAGTAG AGGAGGTTCC AACCAAACAC 
    AGCCAGACAG AGAATTTAGT AGAAGACAGG CAATCACAGT ATTCAATAGA ACACAGAAAA 
    TCACAGACAT TAGTAGAAGA CAGTCCAACA CATGATTTTG TTGAACTTTG TCAATCAGGG 
    CACTTAGCAG AACACAGCCA GTCAGATTGT TTGATAGAAT GCAGTCAATC TACGAATTCA 
    GTTAAACACA GCCAATCACA GGACACAGCA GAGCACAACC AATCAGAAGC TTCTGTAATC 
    TGCAGTCAAT CAAAGAATTC AATAGAACAC GGTCCATCAC AGGCCTCAGT AGAGAGGTCA 
    TCGCAAGATT TTGTTGAAGA CTCACTAGAA CACAGTCAGT CACATGATAT CGCAGAGTGT 
    ATTCTGTCGC AAAATGTAAT GGAACACAGC AAATCACAAG TCTTAGATGA TGACAGGCCA 
    TCACAAGTTT ATGTTGGAGA CAACCAATCA GATAAATCTG TAGAAGTCAA CATTTCACAA 
    GATTCAGAAG AAGACAGGTC ATTAACAGAT TTTGTAGTAC ACAGCCAATC AGAGAACTCA 
    GTAGAACACA GCCAGTCACA GGATTTAGTA GAACCTAGGC AATCACATGA TTTAATAGAA 
    CACTTCAAAT CACTAGACAA CACAGAGCAC AAGCAATCAG ACATGAGTTA TATAAACTGC 
    AGTCAATCAC AGGCCTCAGT AGAAATCTTG CAATCACACG AGTCTGTTGA ACACAGCCAA 
    TCAGAACAGT CAGTAAAACA CAGCCAGTCA CAAGACACTG ACAATTTTAG CCAGACACAG 
    GATTTAATTA AGCACAGCCA ATCACAGACT TTCATAAAAT GCAAACACAA CCAATCACTG 
    GATGGCGTAC AATATGATCA GTCAAAGATC TTAATAGAAC AAACCCAATC ACAGGATTCC 
    GTAAAGGACA ATCCATCCCA AGATCTTGTC AAACATAGCC AATCACAGGA CACAGTAGAA 
    CCGAGTCAAT CACAGGTCTC AGTAGAACAC ATCCAATCAC AGGATTCCCA AGACTGCAGC 
    CATTCACAGA ACTCAACAGT ACACAGCCAA TCACAAACTT CAGTGGGACA AAGCCAATCC 
    CAGGATTTGG TAGAACAAAG TCGATCACCA GCTGCAGCCG AAAACAGTCA ATCACAGACT 
    TCAGTGGAAC ACAGCCAATC ACTGGATTCA GTGGAACACA TGAAATCTCA GACTTCGGTG 
    GAACACAGGC AATCACAGGT TGAATTGATT AAGGCACATT CCATGAACAC CCGGTGGATG 
    GTGACTGAGT CAGACACAGT AGCGTTTTAT GACAAGGTGC GATTAAAACA AGACTGCATG 
    GATGTTTGCG CAGATTGTGA AGTCGTTGAA TCCTTGGGAG TTTTATCAAA ACATGCTGAC 
    AATTTGAACT TCGAAAATAA CGAAAGACTA AATTTCAAAA CACAGGACTT AAATGACACT 
    TCACCTGTTG TTTCTGAATA CGAGTGTTTC CCACATGTGC CTAATGCATC TTCTATTGGA 
    GTGCCAGATT CACTGCATCA GTGCACGATT AGCAATTCAC CCTCAGACCT GCAGGTTAAA 
    ACTCAGCAGA GTTGGCCAGT ATTACAAATA CACACCGAAA AAGACGATTA CTCAGTTAGT 
    AGGTATCACA TGGATGGTTA CTGTTTGCAG GACAGTGAGC AAACAGAGTT TTACAAAACC 
    TACCAGTGCC CTAACAGTAC CATTAATTTT AATACACAGG GTGAGTTCAC TGAAGCTCGG 
    AAGGTTGTAT CAACTGAAGG TGTATTTACT AGAGGTTGTA TCAACACCAA ACTTTTTGAT 
    GGCAAAAGTG TTGACATTGT AGGAGGACCG CAGAAGGACA GCAACAAGCA TCACGTTATG 
    AAAGGGAAAG AATGTACAAT AGTTCCTTCA ATAGAGCAGT CAGTAATTTA CTCTGACAGT 
    TCAGGATGCA TAGAATATAA AAGGCCCAAA ACGTTGAGCA TTGCTTGTCC TGACATGACG 
    ACTGCTGAAT CTGAAGGGTG TCCTGTAGAC AGTCCTGTTG AATCACTTTC TACACTGTTT 
    GAGATTCCAC ATGACAGATC TACAGTCAGC CCCACAGACA TTTGCTTTGA GTTTAGTGAT 
    GGCCCTCATA TGAACTGTAC ACAAGTAGCA CAGAGTGTTA TAGAATATTC AGATGCCCCC 
    AAACTATTTC ACCAGTTTGA CACCCTGTCT GACAAACAAA GAAGACCTAA TGAGTCAGCT 
    GAAGCAATTG CGTCGAATGT TGATATTTTG TCTGGTGCAC GTTCCGATCC TTTCACAAAT 
    GCACAGACTC CTTTTGATTA TTCCCAGGTG AACAGCAGGA ACTATACACA GAGCTCAGAC 
    ACAAATAATA ACATTAAAGC CATTACTTTA GAAGAGTGTG CCTCGGGAGT ACAAATGTCA 
    TTGCCTAGTT CATTGCAGAA ATACAGTAGG TTTACAAATA ATGCTGAATG CTTAGTCTCA 
    AAAAGGGGTG ATTACAAAAA CATTGATTCT TTTAGTGCTC GTAATACTCT GGCTACCCCC 
    TTCAAAGCAC CTGAAATGAA GACTGAAGTG AACAGCAGAG AAAATGTTGT GAAATCTAGC 
    AGTCACATAG ATGTTACACA GAGGAAGCTC TGTGATTGCT GCGGTGGAAA GGAGTGCCAG 
    CTGCAGACAG CGGGTGTATT TCAGTTCAAG GGCACAGACG CCATCAGTCA GCGCAGAACT 
    GCAGTGTTGC AAACCTTGAG TCCCCTCCAC ACAGATGACA GCACACATGA AGCGGTAAAA 
    GTAGACTCTA CAAGCTGTGA ATTTTCACAT ATGGTCAGTT TGCTTACACA CAAACATCCG 
    AATCTTGTCC AAGAAGACTT CGTATTACCA CTGACTGGAG CTCATAAGGC TGATGATACT 
    CATGTATTAG AAAACATGGA GAGAAAGCAT GAAACTTTCC CAATAAATGC TCTTAAAAGC 
    ACTGAATCAA TATGTGAAAC TTCTGATCCT CTTTTTAAGC AAACATTGCA ATTGCACAAC 
    TCGGAGGTTG AGGGCGCATT TGTTTCATGT TGTCATATTT TGCCCATCAC CAACTTAGAG 
    GCCATTTTAG AGACGGACCG TTCCCCGGAG AGCTCATTTG CAGTAGAAAA TGAACTGGAT 
    GAGCAACAGA GAGGAAGTCC TTTATCAGAA GGTGGATTAA ATGAGGACCT AAGCTCTTGT 
    GAGGATGTCA AAAACCGCCC ACATGACAGC AGTTGTGATA TGCATACAAA AGCCAATGTA 
    GCCAATTTCA TGAATGAACC CGATGCTGGA GAAACAATGA ACTCTTTATC AAATGGGTTT 
    GACAGCAGTG ATGACCATAG TGTTTCCAGT TTGGTGTCAC AAGACCTTGA CTCCAGTACC 
    AACAGTGAGG AGACGGAAGA CACCATGCAG CCATCGTTTG GTCTTCGTGG TAATGCGAGA 
    AATAAAAGAA CACAGTCAGC CAAGGGCTCT ATGTTTTCTG GTTTGATTCG AATGCCCACC 
    TTCCGCAAGC CAAAACGAGA ACTCAAACGC AATAAAAACA GGGTTGATCC GAAAGTTAGG 
    GCATCCAGTG GGGATGGAGA TGAACAAGAT GAGATGAAGA ACAACAGGTC ACCCACATGC 
    AGCAAATCTC ACTCTTCACT GTACAAGACC CCTATATCTC AAAGTGCAGA TCACCTTACT 
    GATATTACAA AGCATTCTGA CCACTCAAAT GATGATGTTT TTGAAAGGGC TTTTGCTTTA 
    AATGTGCAAA ATAGAGAGAA ACACATGCAA CCTCAACACC CGTTTGAGCA GCAGCAGCAG 
    AAAGATGGAG ATGCACTCAG TTTTAGAGCT GGCCAGACAA CAGAGGGGTT GCAACAAAAA 
    AGATGCAAGA GCATTGATAA CTTAAACTTA CGCATGAAGC TGGCATTGGC TCACAAGTCT 
    ATTTCTAGCC TGTTTGAGAC CAGATATCCT GAGAGGGACC AAGAGCAAAT CTTACAGTTA 
    GAGAATGAGG ATGGGAAAAC CAAGCAATCA TGGAGAAAGA CCAAACGATG TAACGAAGCA 
    GAACTTTGCA AAAGAACTCC TGTTCCTGGG ACGGTCTGTG ACAAGTGCAT ACATCAGAGC 
    CAAATTGATT GTGCCTTCTG TGCTTATCAG AACCGGTCCA TATTAACCAA CTTACCAAAT 
    GTCCAAGCAA GATTGAAGAA CACGTGCCAT TCTGACCCGC AGAGTTTGAA ATCTGCACCT 
    CAGGAAGGGT TTGAAGATGG TAAATATCTG GAGGAAGGTG AGCAGCCTTG TGGTGCTTTC 
    TCAGATTCAG ACCAAGCATC GGTAGATGGT TTTGAGTCCA GTGTAGAGGA CAGGTCGCTG 
    TCACCTGCAC ACCCCACTGT TCTTGCACTT TCAAACCAAA TGTCACCTAC CTGGACAAGG 
    TCCCTAAGCT GCTTCGAGAC AACTGATACA CCGACAAGGC CCATGAGCCC CAAACCACAC 
    AGTCCTGGTC TGGGCTGGAC ACAGCGGAGG AGCTTCCGCT ACCCCTCAAG ATCTGTTGCA 
    TCTTCTCTCT GCTTGCTTGG TCAGGGAGTG AGCACTGACG GTCTCTTAGA CCCACCTCAG 
    AGGCCGACAT CTCTAAAACC AAGGACAACA CAACTCACCA CAGCTCACTC CTTCGATTCT 
    GAGTACTTAC TGGAGGACAG CAGTTCAGAC AACCAGTCTC AGAATAGCCT GAATTCAGCC 
    TCCCTGATCA GCAAAACAGA GCATGTACAG CACATTACTG AAGCAGAGAG AAGACCACAA 
    GAAAATGCAT GTGTGCTTCG GGTGAAGTCC AGAGATCCAG GTACAGCTGC GCCCAGGCCT 
    ATGTCAGACC TGTACGGCTG GACCATGTCT CTTCAGGGCA CCAGAGAGTT GGCGCTCGAC 
    TTGGAGAGGA AGACTAACGC ACATCAGCGC TCATGCTCAT ATGAAACATT GACCGATGTG 
    GATAACATCC GCAAAAAGAA GCTAAGCAAG CAGCAAAGTT TGCGGCAGAT TAGCACATCA 
    ACATCTGAGA GGCATGAGAA ATTGCGAGCT AGACTTTCCC TCACATCCCC GGAGCAGTTC 
    CCCTCTGTTC CTCTCAAAGA CCACTTCTTC TCTCAGAGCA CACCTGTTGG GCTGGACTAT 
    CTGGGCTGGG CTCACCGTGC ATCCTTTTCA GAAACGAACC TCAGCGCACC GGAAAAAGAG 
    GCACATGGAC AGGTCGCACC TCTCGCATTG GTGATAACTG ATGGGCCACA GGATAAGTCT 
    GGTCTTGCAG ATGAGGTGGG CAGTGAAGAT GATCTGTACA ATGAATTCCG CAGCTCCTCC 
    AACCGATTCG GACACCCAGG AGGAGGAGGA GAACAGCTGG CCATAAATGA GCTGATCAGT 
    GATGGCAGTG TGTGCGCGGA GGCTCTATGG GACCACGTCA CCATGGATGA CCAGGAGCTG 
    GGCTTTAAAG CTGGAGATGT CATTGAAGTA GTGGACGCTA CGAACAAGGA GTGGTGGTGG 
    GGCAGGGTGC TGGACAGCGA GGGCTGGTTC CCCGCCAGCT TTGTTCGGTT ACGGGTGAAC 
    CAAGATGAGC CCATGGAGGA ATACCTAGCC CATCTGGACG GGGCCTCAGA GGGGTGTGGG 
    GCCAGCATGG GGGGACCCTT GGGACCTGGA CTGCCATGCA AGGAGCAGAT GAGAGCCAAC 
    GTCATCAATG AGATCATGAG CACAGAGAGA GATTACATTA AACACCTGAA GGACATATGT 
    GAGGGCTATA TAAAACAATG TCGGAAGAGG ACAGATATGT TTACAGAAGA GCAACTGCGC 
    ACTATCTTTG GCAACATCGA CGAGCTCTAC CGATTCCAGA AGAAGTTCCT CAAAGCCTTG 
    GAGAAAAAAT TCAACAAAGA GCATCCTCAC CTCAGCGAGA TCGGCTCCTG CTTCTTAGAG 
    CAGCAAACAA ACTTCCAGAT CTATTCTGAG TATTGCAACA ACCACCCCAA TGCCTGCGTG 
    CAGCTCTCCA AACTGATGAA GATCAAGAAG TATGTGTTCT TCTTTGAGGC TTGTCGCCTG 
    CTCCAGAAGA TGATTGACAT TTCGTTGGAT GGATTCCTAC TTACTCCTGT TCAGAAGATC 
    TGCAAGTATC CTCTGCAGCT CGCTGAACTG CTCAAGTACA CCAACCCACA GCACAGAGAT 
    TATAAGGATG TGGAAGCTGC CTTAAACGCT ATGAAGAATG TGGCCAGACT GATCAATGAG 
    AGAAAGAGGC GTCTAGAAAA TATTGACAAA ATCGCTCAGT GGCAGAGCTC CATAGAAGAC 
    TGGGAGGGTG AAGACGTCTT GAGCAGGAGC TCTGATCTGA TCTTTTCTGG AGACCTGACC 
    AAGATCTCCC AGCCACAGGC TAAAGGCCAG CAGAGAATGT TCTTCCTCTT TGACCACCAA 
    CTGGTTTTCT GTAAGAAGGA TCTGCTTCGG AGGGATATCT TGTACTACAA GGGTCGGTTA 
    GACATGGATG AGATGGAGGT GGTGGATGTG GAGGATGGGA AGGATAAGGA CTTTAATGTG 
    AGTGTGAAGA ATGCCTTGAA ATTGTGTTCT CCTGGAGGAG AGGAGGTTCA TCTCCTGTGT 
    GCCAAGAAGC CCGAACAAAA GCAGCGCTGG CTGAGAGCCT TCACAGATGA GCGGGAGCAG 
    GTCCAGCATG ACCTCGAGAC AGGTTTTACG ATTACAGAGG TCCAGAAGAA GCAGGCAATG 
    TTGAATGCAA CTAAGAGTCA TCCAACAGGG AAGCCTAAAG CATTAGCTTT ACAGCACCTT 
    TTGGCACTCA GAGCTCCACT CCTTCACCCC AAAACCAAGT GCCTGCGTTT GTCCGAGCCT 
    CCAGGACCTC TGGTTCCCAG GGCCACCCTA ACTTCACCTC TCCTCCCTGA AGGGCCTCAG 
    CGCAGGCAGG GGCGGGGGGT TATGGTGCCC CCTGGGGTCC TGCGCAGGGC TATATCCTGG 
    GTCTCCTCTC AAAGTAGAGA GCCCATCGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322704.1
    CDS347..9559
    Translation

    Target ORF information:

    RefSeq Version XM_021467029.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021467029.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAATCACA GAACTCTGCT GATCACAGCC AGTCACAAGC TTCTTTAAAC 
    TGCAGCCAAC CACAACTCTC AGAAGAATTC AGGCCATCAC AAGATTTTGT TGAACACAAC 
    CAATCAAATA AAACTGTAGA TCTCAACCAG TCACAGGATT CAGTAAAACA CAGTCAGTTG 
    CAGGATACAA TGGAATACAG TAAAACACAA GCCTCAGTAG AGGAGGTTCC AACCAAACAC 
    AGCCAGACAG AGAATTTAGT AGAAGACAGG CAATCACAGT ATTCAATAGA ACACAGAAAA 
    TCACAGACAT TAGTAGAAGA CAGTCCAACA CATGATTTTG TTGAACTTTG TCAATCAGGG 
    CACTTAGCAG AACACAGCCA GTCAGATTGT TTGATAGAAT GCAGTCAATC TACGAATTCA 
    GTTAAACACA GCCAATCACA GGACACAGCA GAGCACAACC AATCAGAAGC TTCTGTAATC 
    TGCAGTCAAT CAAAGAATTC AATAGAACAC GGTCCATCAC AGGCCTCAGT AGAGAGGTCA 
    TCGCAAGATT TTGTTGAAGA CTCACTAGAA CACAGTCAGT CACATGATAT CGCAGAGTGT 
    ATTCTGTCGC AAAATGTAAT GGAACACAGC AAATCACAAG TCTTAGATGA TGACAGGCCA 
    TCACAAGTTT ATGTTGGAGA CAACCAATCA GATAAATCTG TAGAAGTCAA CATTTCACAA 
    GATTCAGAAG AAGACAGGTC ATTAACAGAT TTTGTAGTAC ACAGCCAATC AGAGAACTCA 
    GTAGAACACA GCCAGTCACA GGATTTAGTA GAACCTAGGC AATCACATGA TTTAATAGAA 
    CACTTCAAAT CACTAGACAA CACAGAGCAC AAGCAATCAG ACATGAGTTA TATAAACTGC 
    AGTCAATCAC AGGCCTCAGT AGAAATCTTG CAATCACACG AGTCTGTTGA ACACAGCCAA 
    TCAGAACAGT CAGTAAAACA CAGCCAGTCA CAAGACACTG ACAATTTTAG CCAGACACAG 
    GATTTAATTA AGCACAGCCA ATCACAGACT TTCATAAAAT GCAAACACAA CCAATCACTG 
    GATGGCGTAC AATATGATCA GTCAAAGATC TTAATAGAAC AAACCCAATC ACAGGATTCC 
    GTAAAGGACA ATCCATCCCA AGATCTTGTC AAACATAGCC AATCACAGGA CACAGTAGAA 
    CCGAGTCAAT CACAGGTCTC AGTAGAACAC ATCCAATCAC AGGATTCCCA AGACTGCAGC 
    CATTCACAGA ACTCAACAGT ACACAGCCAA TCACAAACTT CAGTGGGACA AAGCCAATCC 
    CAGGATTTGG TAGAACAAAG TCGATCACCA GCTGCAGCCG AAAACAGTCA ATCACAGACT 
    TCAGTGGAAC ACAGCCAATC ACTGGATTCA GTGGAACACA TGAAATCTCA GACTTCGGTG 
    GAACACAGGC AATCACAGGT TGAATTGATT AAGGCACATT CCATGAACAC CCGGTGGATG 
    GTGACTGAGT CAGACACAGT AGCGTTTTAT GACAAGGTGC GATTAAAACA AGACTGCATG 
    GATGTTTGCG CAGATTGTGA AGTCGTTGAA TCCTTGGGAG TTTTATCAAA ACATGCTGAC 
    AATTTGAACT TCGAAAATAA CGAAAGACTA AATTTCAAAA CACAGGACTT AAATGACACT 
    TCACCTGTTG TTTCTGAATA CGAGTGTTTC CCACATGTGC CTAATGCATC TTCTATTGGA 
    GTGCCAGATT CACTGCATCA GTGCACGATT AGCAATTCAC CCTCAGACCT GCAGGTTAAA 
    ACTCAGCAGA GTTGGCCAGT ATTACAAATA CACACCGAAA AAGACGATTA CTCAGTTAGT 
    AGGTATCACA TGGATGGTTA CTGTTTGCAG GACAGTGAGC AAACAGAGTT TTACAAAACC 
    TACCAGTGCC CTAACAGTAC CATTAATTTT AATACACAGG GTGAGTTCAC TGAAGCTCGG 
    AAGGTTGTAT CAACTGAAGG TGTATTTACT AGAGGTTGTA TCAACACCAA ACTTTTTGAT 
    GGCAAAAGTG TTGACATTGT AGGAGGACCG CAGAAGGACA GCAACAAGCA TCACGTTATG 
    AAAGGGAAAG AATGTACAAT AGTTCCTTCA ATAGAGCAGT CAGTAATTTA CTCTGACAGT 
    TCAGGATGCA TAGAATATAA AAGGCCCAAA ACGTTGAGCA TTGCTTGTCC TGACATGACG 
    ACTGCTGAAT CTGAAGGGTG TCCTGTAGAC AGTCCTGTTG AATCACTTTC TACACTGTTT 
    GAGATTCCAC ATGACAGATC TACAGTCAGC CCCACAGACA TTTGCTTTGA GTTTAGTGAT 
    GGCCCTCATA TGAACTGTAC ACAAGTAGCA CAGAGTGTTA TAGAATATTC AGATGCCCCC 
    AAACTATTTC ACCAGTTTGA CACCCTGTCT GACAAACAAA GAAGACCTAA TGAGTCAGCT 
    GAAGCAATTG CGTCGAATGT TGATATTTTG TCTGGTGCAC GTTCCGATCC TTTCACAAAT 
    GCACAGACTC CTTTTGATTA TTCCCAGGTG AACAGCAGGA ACTATACACA GAGCTCAGAC 
    ACAAATAATA ACATTAAAGC CATTACTTTA GAAGAGTGTG CCTCGGGAGT ACAAATGTCA 
    TTGCCTAGTT CATTGCAGAA ATACAGTAGG TTTACAAATA ATGCTGAATG CTTAGTCTCA 
    AAAAGGGGTG ATTACAAAAA CATTGATTCT TTTAGTGCTC GTAATACTCT GGCTACCCCC 
    TTCAAAGCAC CTGAAATGAA GACTGAAGTG AACAGCAGAG AAAATGTTGT GAAATCTAGC 
    AGTCACATAG ATGTTACACA GAGGAAGCTC TGTGATTGCT GCGGTGGAAA GGAGTGCCAG 
    CTGCAGACAG CGGGTGTATT TCAGTTCAAG GGCACAGACG CCATCAGTCA GCGCAGAACT 
    GCAGTGTTGC AAACCTTGAG TCCCCTCCAC ACAGATGACA GCACACATGA AGCGGTAAAA 
    GTAGACTCTA CAAGCTGTGA ATTTTCACAT ATGGTCAGTT TGCTTACACA CAAACATCCG 
    AATCTTGTCC AAGAAGACTT CGTATTACCA CTGACTGGAG CTCATAAGGC TGATGATACT 
    CATGTATTAG AAAACATGGA GAGAAAGCAT GAAACTTTCC CAATAAATGC TCTTAAAAGC 
    ACTGAATCAA TATGTGAAAC TTCTGATCCT CTTTTTAAGC AAACATTGCA ATTGCACAAC 
    TCGGAGGTTG AGGGCGCATT TGTTTCATGT TGTCATATTT TGCCCATCAC CAACTTAGAG 
    GCCATTTTAG AGACGGACCG TTCCCCGGAG AGCTCATTTG CAGTAGAAAA TGAACTGGAT 
    GAGCAACAGA GAGGAAGTCC TTTATCAGAA GGTGGATTAA ATGAGGACCT AAGCTCTTGT 
    GAGGATGTCA AAAACCGCCC ACATGACAGC AGTTGTGATA TGCATACAAA AGCCAATGTA 
    GCCAATTTCA TGAATGAACC CGATGCTGGA GAAACAATGA ACTCTTTATC AAATGGGTTT 
    GACAGCAGTG ATGACCATAG TGTTTCCAGT TTGGTGTCAC AAGACCTTGA CTCCAGTACC 
    AACAGTGAGG AGACGGAAGA CACCATGCAG CCATCGTTTG GTCTTCGTGG TAATGCGAGA 
    AATAAAAGAA CACAGTCAGC CAAGGGCTCT ATGTTTTCTG GTTTGATTCG AATGCCCACC 
    TTCCGCAAGC CAAAACGAGA ACTCAAACGC AATAAAAACA GGGTTGATCC GAAAGTTAGG 
    GCATCCAGTG GGGATGGAGA TGAACAAGAT GAGATGAAGA ACAACAGGTC ACCCACATGC 
    AGCAAATCTC ACTCTTCACT GTACAAGACC CCTATATCTC AAAGTGCAGA TCACCTTACT 
    GATATTACAA AGCATTCTGA CCACTCAAAT GATGATGTTT TTGAAAGGGC TTTTGCTTTA 
    AATGTGCAAA ATAGAGAGAA ACACATGCAA CCTCAACACC CGTTTGAGCA GCAGCAGCAG 
    AAAGATGGAG ATGCACTCAG TTTTAGAGCT GGCCAGACAA CAGAGGGGTT GCAACAAAAA 
    AGATGCAAGA GCATTGATAA CTTAAACTTA CGCATGAAGC TGGCATTGGC TCACAAGTCT 
    ATTTCTAGCC TGTTTGAGAC CAGATATCCT GAGAGGGACC AAGAGCAAAT CTTACAGTTA 
    GAGAATGAGG ATGGGAAAAC CAAGCAATCA TGGAGAAAGA CCAAACGATG TAACGAAGCA 
    GAACTTTGCA AAAGAACTCC TGTTCCTGGG ACGGTCTGTG ACAAGTGCAT ACATCAGAGC 
    CAAATTGATT GTGCCTTCTG TGCTTATCAG AACCGGTCCA TATTAACCAA CTTACCAAAT 
    GTCCAAGCAA GATTGAAGAA CACGTGCCAT TCTGACCCGC AGAGTTTGAA ATCTGCACCT 
    CAGGAAGGGT TTGAAGATGG TAAATATCTG GAGGAAGGTG AGCAGCCTTG TGGTGCTTTC 
    TCAGATTCAG ACCAAGCATC GGTAGATGGT TTTGAGTCCA GTGTAGAGGA CAGGTCGCTG 
    TCACCTGCAC ACCCCACTGT TCTTGCACTT TCAAACCAAA TGTCACCTAC CTGGACAAGG 
    TCCCTAAGCT GCTTCGAGAC AACTGATACA CCGACAAGGC CCATGAGCCC CAAACCACAC 
    AGTCCTGGTC TGGGCTGGAC ACAGCGGAGG AGCTTCCGCT ACCCCTCAAG ATCTGTTGCA 
    TCTTCTCTCT GCTTGCTTGG TCAGGGAGTG AGCACTGACG GTCTCTTAGA CCCACCTCAG 
    AGGCCGACAT CTCTAAAACC AAGGACAACA CAACTCACCA CAGCTCACTC CTTCGATTCT 
    GAGTACTTAC TGGAGGACAG CAGTTCAGAC AACCAGTCTC AGAATAGCCT GAATTCAGCC 
    TCCCTGATCA GCAAAACAGA GCATGTACAG CACATTACTG AAGCAGAGAG AAGACCACAA 
    GAAAATGCAT GTGTGCTTCG GGTGAAGTCC AGAGATCCAG GTACAGCTGC GCCCAGGCCT 
    ATGTCAGACC TGTACGGCTG GACCATGTCT CTTCAGGGCA CCAGAGAGTT GGCGCTCGAC 
    TTGGAGAGGA AGACTAACGC ACATCAGCGC TCATGCTCAT ATGAAACATT GACCGATGTG 
    GATAACATCC GCAAAAAGAA GCTAAGCAAG CAGCAAAGTT TGCGGCAGAT TAGCACATCA 
    ACATCTGAGA GGCATGAGAA ATTGCGAGCT AGACTTTCCC TCACATCCCC GGAGCAGTTC 
    CCCTCTGTTC CTCTCAAAGA CCACTTCTTC TCTCAGAGCA CACCTGTTGG GCTGGACTAT 
    CTGGGCTGGG CTCACCGTGC ATCCTTTTCA GAAACGAACC TCAGCGCACC GGAAAAAGAG 
    GCACATGGAC AGGTCGCACC TCTCGCATTG GTGATAACTG ATGGGCCACA GGATAAGTCT 
    GGTCTTGCAG ATGAGGTGGG CAGTGAAGAT GATCTGTACA ATGAATTCCG CAGCTCCTCC 
    AACCGATTCG GACACCCAGG AGGAGGAGGA GAACAGCTGG CCATAAATGA GCTGATCAGT 
    GATGGCAGTG TGTGCGCGGA GGCTCTATGG GACCACGTCA CCATGGATGA CCAGGAGCTG 
    GGCTTTAAAG CTGGAGATGT CATTGAAGTA GTGGACGCTA CGAACAAGGA GTGGTGGTGG 
    GGCAGGGTGC TGGACAGCGA GGGCTGGTTC CCCGCCAGCT TTGTTCGGTT ACGGGTGAAC 
    CAAGATGAGC CCATGGAGGA ATACCTAGCC CATCTGGACG GGGCCTCAGA GGGGTGTGGG 
    GCCAGCATGG GGGGACCCTT GGGACCTGGA CTGCCATGCA AGGAGCAGAT GAGAGCCAAC 
    GTCATCAATG AGATCATGAG CACAGAGAGA GATTACATTA AACACCTGAA GGACATATGT 
    GAGGGCTATA TAAAACAATG TCGGAAGAGG ACAGATATGT TTACAGAAGA GCAACTGCGC 
    ACTATCTTTG GCAACATCGA CGAGCTCTAC CGATTCCAGA AGAAGTTCCT CAAAGCCTTG 
    GAGAAAAAAT TCAACAAAGA GCATCCTCAC CTCAGCGAGA TCGGCTCCTG CTTCTTAGAG 
    CAGCAAACAA ACTTCCAGAT CTATTCTGAG TATTGCAACA ACCACCCCAA TGCCTGCGTG 
    CAGCTCTCCA AACTGATGAA GATCAAGAAG TATGTGTTCT TCTTTGAGGC TTGTCGCCTG 
    CTCCAGAAGA TGATTGACAT TTCGTTGGAT GGATTCCTAC TTACTCCTGT TCAGAAGATC 
    TGCAAGTATC CTCTGCAGCT CGCTGAACTG CTCAAGTACA CCAACCCACA GCACAGAGAT 
    TATAAGGATG TGGAAGCTGC CTTAAACGCT ATGAAGAATG TGGCCAGACT GATCAATGAG 
    AGAAAGAGGC GTCTAGAAAA TATTGACAAA ATCGCTCAGT GGCAGAGCTC CATAGAAGAC 
    TGGGAGGGTG AAGACGTCTT GAGCAGGAGC TCTGATCTGA TCTTTTCTGG AGACCTGACC 
    AAGATCTCCC AGCCACAGGC TAAAGGCCAG CAGAGAATGT TCTTCCTCTT TGACCACCAA 
    CTGGTTTTCT GTAAGAAGGA TCTGCTTCGG AGGGATATCT TGTACTACAA GGGTCGGTTA 
    GACATGGATG AGATGGAGGT GGTGGATGTG GAGGATGGGA AGGATAAGGA CTTTAATGTG 
    AGTGTGAAGA ATGCCTTGAA ATTGTGTTCT CCTGGAGGAG AGGAGGTTCA TCTCCTGTGT 
    GCCAAGAAGC CCGAACAAAA GCAGCGCTGG CTGAGAGCCT TCACAGATGA GCGGGAGCAG 
    GTCCAGCATG ACCTCGAGAC AGGTTTTACG ATTACAGAGG TCCAGAAGAA GCAGGCAATG 
    TTGAATGCAA CTAAGAGTCA TCCAACAGGG AAGCCTAAAG CATTAGCTTT ACAGCACCTT 
    TTGGCACTCA GAGCTCCACT CCTTCACCCC AAAACCAAGT GCCTGCGTTT GTCCGAGCCT 
    CCAGGACCTC TGGTTCCCAG GGCCACCCTA ACTTCACCTC TCCTCCCTGA AGGGCCTCAG 
    CGCAGGCAGG GGCGGGGGGT TATGGTGCCC CCTGGGGTCC TGCGCAGGGC TATATCCTGG 
    GTCTCCTCTC AAAGTAGAGA GCCCATCGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_021467031.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322706.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa150442
    Clone ID Related Accession (Same CDS sequence) XM_021467031.1
    Accession Version XM_021467031.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA ACCACAGATA 
    TCAGTAGAGC ACAGCCAACC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAT 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAT TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA TCCACAGATA TCAGTAGAGC ACAGCCGAAC ACATATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAACC AACCACAGAT GTCAGTAGAG 
    CACAACCAAC CGCAGATGTC AGTAGACCAC AGCCAACCAC AGATATCAGT AGACCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGCAGAGCA CAGGCATTCA 
    CAGATGTCAG TAGAGCACAG CCAAGCACAG ATGTCAGTAG ACCACAGTCA ACCACAGATG 
    TCAGTAGGCC ACAGTCAATC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATGTC AGCAGAGCAC 
    AGGCAATCAC AGATGTCAGT AGAGCACAGC CAACCTCAGA TGTTAGTAGA GCACAGCCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAATCA CAGATGTCAG TAGAGCACAG CCAATCGCAG 
    ATGTCAGTAG AGCACAGCCA ACCACAGATG TTAGTAGAGC ACAGCCAACC ACAGTTATTT 
    GTAGAGCACA GCCAATCACA GAACTCTGCT GATCACAGCC AGTCACAAGC TTCTTTAAAC 
    TGCAGCCAAC CACAACTCTC AGAAGAATTC AGGCCATCAC AAGATTTTGT TGAACACAAC 
    CAATCAAATA AAACTGTAGA TCTCAACCAG TCACAGGATT CAGTAAAACA CAGTCAGTTG 
    CAGGATACAA TGGAATACAG TAAAACACAA GCCTCAGTAG AGGAGGTTCC AACCAAACAC 
    AGCCAGACAG AGAATTTAGT AGAAGACAGG CAATCACAGT ATTCAATAGA ACACAGAAAA 
    TCACAGACAT TAGTAGAAGA CAGTCCAACA CATGATTTTG TTGAACTTTG TCAATCAGGG 
    CACTTAGCAG AACACAGCCA GTCAGATTGT TTGATAGAAT GCAGTCAATC TACGAATTCA 
    GTTAAACACA GCCAATCACA GGACACAGCA GAGCACAACC AATCAGAAGC TTCTGTAATC 
    TGCAGTCAAT CAAAGAATTC AATAGAACAC GGTCCATCAC AGGCCTCAGT AGAGAGGTCA 
    TCGCAAGATT TTGTTGAAGA CTCACTAGAA CACAGTCAGT CACATGATAT CGCAGAGTGT 
    ATTCTGTCGC AAAATGTAAT GGAACACAGC AAATCACAAG TCTTAGATGA TGACAGGCCA 
    TCACAAGTTT ATGTTGGAGA CAACCAATCA GATAAATCTG TAGAAGTCAA CATTTCACAA 
    GATTCAGAAG AAGACAGGTC ATTAACAGAT TTTGTAGTAC ACAGCCAATC AGAGAACTCA 
    GTAGAACACA GCCAGTCACA GGATTTAGTA GAACCTAGGC AATCACATGA TTTAATAGAA 
    CACTTCAAAT CACTAGACAA CACAGAGCAC AAGCAATCAG ACATGAGTTA TATAAACTGC 
    AGTCAATCAC AGGCCTCAGT AGAAATCTTG CAATCACACG AGTCTGTTGA ACACAGCCAA 
    TCAGAACAGT CAGTAAAACA CAGCCAGTCA CAAGACACTG ACAATTTTAG CCAGACACAG 
    GATTTAATTA AGCACAGCCA ATCACAGACT TTCATAAAAT GCAAACACAA CCAATCACTG 
    GATGGCGTAC AATATGATCA GTCAAAGATC TTAATAGAAC AAACCCAATC ACAGGATTCC 
    GTAAAGGACA ATCCATCCCA AGATCTTGTC AAACATAGCC AATCACAGGA CACAGTAGAA 
    CCGAGTCAAT CACAGGTCTC AGTAGAACAC ATCCAATCAC AGGATTCCCA AGACTGCAGC 
    CATTCACAGA ACTCAACAGT ACACAGCCAA TCACAAACTT CAGTGGGACA AAGCCAATCC 
    CAGGATTTGG TAGAACAAAG TCGATCACCA GCTGCAGCCG AAAACAGTCA ATCACAGACT 
    TCAGTGGAAC ACAGCCAATC ACTGGATTCA GTGGAACACA TGAAATCTCA GACTTCGGTG 
    GAACACAGGC AATCACAGGT TGAATTGATT AAGGCACATT CCATGAACAC CCGGTGGATG 
    GTGACTGAGT CAGACACAGT AGCGTTTTAT GACAAGGTGC GATTAAAACA AGACTGCATG 
    GATGTTTGCG CAGATTGTGA AGTCGTTGAA TCCTTGGGAG TTTTATCAAA ACATGCTGAC 
    AATTTGAACT TCGAAAATAA CGAAAGACTA AATTTCAAAA CACAGGACTT AAATGACACT 
    TCACCTGTTG TTTCTGAATA CGAGTGTTTC CCACATGTGC CTAATGCATC TTCTATTGGA 
    GTGCCAGATT CACTGCATCA GTGCACGATT AGCAATTCAC CCTCAGACCT GCAGGTTAAA 
    ACTCAGCAGA GTTGGCCAGT ATTACAAATA CACACCGAAA AAGACGATTA CTCAGTTAGT 
    AGGTATCACA TGGATGGTTA CTGTTTGCAG GACAGTGAGC AAACAGAGTT TTACAAAACC 
    TACCAGTGCC CTAACAGTAC CATTAATTTT AATACACAGG GTGAGTTCAC TGAAGCTCGG 
    AAGGTTGTAT CAACTGAAGG TGTATTTACT AGAGGTTGTA TCAACACCAA ACTTTTTGAT 
    GGCAAAAGTG TTGACATTGT AGGAGGACCG CAGAAGGACA GCAACAAGCA TCACGTTATG 
    AAAGGGAAAG AATGTACAAT AGTTCCTTCA ATAGAGCAGT CAGTAATTTA CTCTGACAGT 
    TCAGGATGCA TAGAATATAA AAGGCCCAAA ACGTTGAGCA TTGCTTGTCC TGACATGACG 
    ACTGCTGAAT CTGAAGGGTG TCCTGTAGAC AGTCCTGTTG AATCACTTTC TACACTGTTT 
    GAGATTCCAC ATGACAGATC TACAGTCAGC CCCACAGACA TTTGCTTTGA GTTTAGTGAT 
    GGCCCTCATA TGAACTGTAC ACAAGTAGCA CAGAGTGTTA TAGAATATTC AGATGCCCCC 
    AAACTATTTC ACCAGTTTGA CACCCTGTCT GACAAACAAA GAAGACCTAA TGAGTCAGCT 
    GAAGCAATTG CGTCGAATGT TGATATTTTG TCTGGTGCAC GTTCCGATCC TTTCACAAAT 
    GCACAGACTC CTTTTGATTA TTCCCAGGTG AACAGCAGGA ACTATACACA GAGCTCAGAC 
    ACAAATAATA ACATTAAAGC CATTACTTTA GAAGAGTGTG CCTCGGGAGT ACAAATGTCA 
    TTGCCTAGTT CATTGCAGAA ATACAGTAGG TTTACAAATA ATGCTGAATG CTTAGTCTCA 
    AAAAGGGGTG ATTACAAAAA CATTGATTCT TTTAGTGCTC GTAATACTCT GGCTACCCCC 
    TTCAAAGCAC CTGAAATGAA GACTGAAGTG AACAGCAGAG AAAATGTTGT GAAATCTAGC 
    AGTCACATAG ATGTTACACA GAGGAAGCTC TGTGATTGCT GCGGTGGAAA GGAGTGCCAG 
    CTGCAGACAG CGGGTGTATT TCAGTTCAAG GGCACAGACG CCATCAGTCA GCGCAGAACT 
    GCAGTGTTGC AAACCTTGAG TCCCCTCCAC ACAGATGACA GCACACATGA AGCGGTAAAA 
    GTAGACTCTA CAAGCTGTGA ATTTTCACAT ATGGTCAGTT TGCTTACACA CAAACATCCG 
    AATCTTGTCC AAGAAGACTT CGTATTACCA CTGACTGGAG CTCATAAGGC TGATGATACT 
    CATGTATTAG AAAACATGGA GAGAAAGCAT GAAACTTTCC CAATAAATGC TCTTAAAAGC 
    ACTGAATCAA TATGTGAAAC TTCTGATCCT CTTTTTAAGC AAACATTGCA ATTGCACAAC 
    TCGGAGGTTG AGGGCGCATT TGTTTCATGT TGTCATATTT TGCCCATCAC CAACTTAGAG 
    GCCATTTTAG AGACGGACCG TTCCCCGGAG AGCTCATTTG CAGTAGAAAA TGAACTGGAT 
    GAGCAACAGA GAGGAAGTCC TTTATCAGAA GGTGGATTAA ATGAGGACCT AAGCTCTTGT 
    GAGGATGTCA AAAACCGCCC ACATGACAGC AGTTGTGATA TGCATACAAA AGCCAATGTA 
    GCCAATTTCA TGAATGAACC CGATGCTGGA GAAACAATGA ACTCTTTATC AAATGGGTTT 
    GACAGCAGTG ATGACCATAG TGTTTCCAGT TTGGTGTCAC AAGACCTTGA CTCCAGTACC 
    AACAGTGAGG AGACGGAAGA CACCATGCAG CCATCGTTTG GTCTTCGTGG TAATGCGAGA 
    AATAAAAGAA CACAGTCAGC CAAGGGCTCT ATGTTTTCTG GTTTGATTCG AATGCCCACC 
    TTCCGCAAGC CAAAACGAGA ACTCAAACGC AATAAAAACA GGGTTGATCC GAAAGTTAGG 
    GCATCCAGTG GGGATGGAGA TGAACAAGAT GAGATGAAGA ACAACAGGTC ACCCACATGC 
    AGCAAATCTC ACTCTTCACT GTACAAGACC CCTATATCTC AAAGTGCAGA TCACCTTACT 
    GATATTACAA AGCATTCTGA CCACTCAAAT GATGATGTTT TTGAAAGGGC TTTTGCTTTA 
    AATGTGCAAA ATAGAGAGAA ACACATGCAA CCTCAACACC CGTTTGAGCA GCAGCAGCAG 
    AAAGATGGAG ATGCACTCAG TTTTAGAGCT GGCCAGACAA CAGAGGGGTT GCAACAAAAA 
    AGATGCAAGA GCATTGATAA CTTAAACTTA CGCATGAAGC TGGCATTGGC TCACAAGTCT 
    ATTTCTAGCC TGTTTGAGAC CAGATATCCT GAGAGGGACC AAGAGCAAAT CTTACAGTTA 
    GAGAATGAGG ATGGGAAAAC CAAGCAATCA TGGAGAAAGA CCAAACGATG TAACGAAGCA 
    GAACTTTGCA AAAGAACTCC TGTTCCTGGG ACGGTCTGTG ACAAGTGCAT ACATCAGAGC 
    CAAATTGATT GTGCCTTCTG TGCTTATCAG AACCGGTCCA TATTAACCAA CTTACCAAAT 
    GTCCAAGCAA GATTGAAGAA CACGTGCCAT TCTGACCCGC AGAGTTTGAA ATCTGCACCT 
    CAGGAAGGGT TTGAAGATGG TAAATATCTG GAGGAAGGTG AGCAGCCTTG TGGTGCTTTC 
    TCAGATTCAG ACCAAGCATC GGTAGATGGT TTTGAGTCCA GTGTAGAGGA CAGGTCGCTG 
    TCACCTGCAC ACCCCACTGT TCTTGCACTT TCAAACCAAA TGTCACCTAC CTGGACAAGG 
    TCCCTAAGCT GCTTCGAGAC AACTGATACA CCGACAAGGC CCATGAGCCC CAAACCACAC 
    AGTCCTGGTC TGGGCTGGAC ACAGCGGAGG AGCTTCCGCT ACCCCTCAAG ATCTGTTGCA 
    TCTTCTCTCT GCTTGCTTGG TCAGGGAGTG AGCACTGACG GTCTCTTAGA CCCACCTCAG 
    AGGCCGACAT CTCTAAAACC AAGGACAACA CAACTCACCA CAGCTCACTC CTTCGATTCT 
    GAGTACTTAC TGGAGGACAG CAGTTCAGAC AACCAGTCTC AGAATAGCCT GAATTCAGCC 
    TCCCTGATCA GCAAAACAGA GCATGTACAG CACATTACTG AAGCAGAGAG AAGACCACAA 
    GAAAATGCAT GTGTGCTTCG GGTGAAGTCC AGAGATCCAG GTACAGCTGC GCCCAGGCCT 
    ATGTCAGACC TGTACGGCTG GACCATGTCT CTTCAGGGCA CCAGAGAGTT GGCGCTCGAC 
    TTGGAGAGGA AGACTAACGC ACATCAGCGC TCATGCTCAT ATGAAACATT GACCGATGTG 
    GATAACATCC GCAAAAAGAA GCTAAGCAAG CAGCAAAGTT TGCGGCAGAT TAGCACATCA 
    ACATCTGAGA GGCATGAGAA ATTGCGAGCT AGACTTTCCC TCACATCCCC GGAGCAGTTC 
    CCCTCTGTTC CTCTCAAAGA CCACTTCTTC TCTCAGAGCA CACCTGTTGG GCTGGACTAT 
    CTGGGCTGGG CTCACCGTGC ATCCTTTTCA GAAACGAACC TCAGCGCACC GGAAAAAGAG 
    GCACATGGAC AGGTCGCACC TCTCGCATTG GTGATAACTG ATGGGCCACA GGATAAGTCT 
    GGTCTTGCAG ATGAGGTGGG CAGTGAAGAT GATCTGTACA ATGAATTCCG CAGCTCCTCC 
    AACCGATTCG GACACCCAGG AGGAGGAGGA GAACAGCTGG CCATAAATGA GCTGATCAGT 
    GATGGCAGTG TGTGCGCGGA GGCTCTATGG GACCACGTCA CCATGGATGA CCAGGAGCTG 
    GGCTTTAAAG CTGGAGATGT CATTGAAGTA GTGGACGCTA CGAACAAGGA GTGGTGGTGG 
    GGCAGGGTGC TGGACAGCGA GGGCTGGTTC CCCGCCAGCT TTGTTCGGTT ACGGGTGAAC 
    CAAGATGAGC CCATGGAGGA ATACCTAGCC CATCTGGACG GGGCCTCAGA GGGGTGTGGG 
    GCCAGCATGG GGGGACCCTT GGGACCTGGA CTGCCATGCA AGGAGCAGAT GAGAGCCAAC 
    GTCATCAATG AGATCATGAG CACAGAGAGA GATTACATTA AACACCTGAA GGACATATGT 
    GAGGGCTATA TAAAACAATG TCGGAAGAGG ACAGATATGT TTACAGAAGA GCAACTGCGC 
    ACTATCTTTG GCAACATCGA CGAGCTCTAC CGATTCCAGA AGAAGTTCCT CAAAGCCTTG 
    GAGAAAAAAT TCAACAAAGA GCATCCTCAC CTCAGCGAGA TCGGCTCCTG CTTCTTAGAG 
    CAGCAAACAA ACTTCCAGAT CTATTCTGAG TATTGCAACA ACCACCCCAA TGCCTGCGTG 
    CAGCTCTCCA AACTGATGAA GATCAAGAAG TATGTGTTCT TCTTTGAGGC TTGTCGCCTG 
    CTCCAGAAGA TGATTGACAT TTCGTTGGAT GGATTCCTAC TTACTCCTGT TCAGAAGATC 
    TGCAAGTATC CTCTGCAGCT CGCTGAACTG CTCAAGTACA CCAACCCACA GCACAGAGAT 
    TATAAGGATG TGGAAGCTGC CTTAAACGCT ATGAAGAATG TGGCCAGACT GATCAATGAG 
    AGAAAGAGGC GTCTAGAAAA TATTGACAAA ATCGCTCAGT GGCAGAGCTC CATAGAAGAC 
    TGGGAGGGTG AAGACGTCTT GAGCAGGAGC TCTGATCTGA TCTTTTCTGG AGACCTGACC 
    AAGATCTCCC AGCCACAGGC TAAAGGCCAG CAGAGAATGT TCTTCCTCTT TGACCACCAA 
    CTGGTTTTCT GTAAGAAGGA TCTGCTTCGG AGGGATATCT TGTACTACAA GGGTCGGTTA 
    GACATGGATG AGATGGAGGT GGTGGATGTG GAGGATGGGA AGGATAAGGA CTTTAATGTG 
    AGTGTGAAGA ATGCCTTGAA ATTGTGTTCT CCTGGAGGAG AGGAGGTTCA TCTCCTGTGT 
    GCCAAGAAGC CCGAACAAAA GCAGCGCTGG CTGAGAGCCT TCACAGATGA GCGGGAGCAG 
    GTCCAGCATG ACCTCGAGAC AGGTTTTACG ATTACAGAGG TCCAGAAGAA GCAGGCAATG 
    TTGAATGCAA CTAAGAGTCA TCCAACAGGG AAGCCTAAAG CATTAGCTTT ACAGCACCTT 
    TTGGCACTCA GAGCTCCACT CCTTCACCCC AAAACCAAGT GCCTGCGTTT GTCCGAGCCT 
    CCAGGACCTC TGGTTCCCAG GGCCACCCTA ACTTCACCTC TCCTCCCTGA AGGGCCTCAG 
    CGCAGGCAGG GGCGGGGGGT TATGGTGCCC CCTGGGGTCC TGCGCAGGGC TATATCCTGG 
    GTCTCCTCTC AAAGTAGAGA GCCCATCGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322706.1
    CDS347..9559
    Translation

    Target ORF information:

    RefSeq Version XM_021467031.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021467031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA ACCACAGATA 
    TCAGTAGAGC ACAGCCAACC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAT 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAT TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA TCCACAGATA TCAGTAGAGC ACAGCCGAAC ACATATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAACC AACCACAGAT GTCAGTAGAG 
    CACAACCAAC CGCAGATGTC AGTAGACCAC AGCCAACCAC AGATATCAGT AGACCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGCAGAGCA CAGGCATTCA 
    CAGATGTCAG TAGAGCACAG CCAAGCACAG ATGTCAGTAG ACCACAGTCA ACCACAGATG 
    TCAGTAGGCC ACAGTCAATC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATGTC AGCAGAGCAC 
    AGGCAATCAC AGATGTCAGT AGAGCACAGC CAACCTCAGA TGTTAGTAGA GCACAGCCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAATCA CAGATGTCAG TAGAGCACAG CCAATCGCAG 
    ATGTCAGTAG AGCACAGCCA ACCACAGATG TTAGTAGAGC ACAGCCAACC ACAGTTATTT 
    GTAGAGCACA GCCAATCACA GAACTCTGCT GATCACAGCC AGTCACAAGC TTCTTTAAAC 
    TGCAGCCAAC CACAACTCTC AGAAGAATTC AGGCCATCAC AAGATTTTGT TGAACACAAC 
    CAATCAAATA AAACTGTAGA TCTCAACCAG TCACAGGATT CAGTAAAACA CAGTCAGTTG 
    CAGGATACAA TGGAATACAG TAAAACACAA GCCTCAGTAG AGGAGGTTCC AACCAAACAC 
    AGCCAGACAG AGAATTTAGT AGAAGACAGG CAATCACAGT ATTCAATAGA ACACAGAAAA 
    TCACAGACAT TAGTAGAAGA CAGTCCAACA CATGATTTTG TTGAACTTTG TCAATCAGGG 
    CACTTAGCAG AACACAGCCA GTCAGATTGT TTGATAGAAT GCAGTCAATC TACGAATTCA 
    GTTAAACACA GCCAATCACA GGACACAGCA GAGCACAACC AATCAGAAGC TTCTGTAATC 
    TGCAGTCAAT CAAAGAATTC AATAGAACAC GGTCCATCAC AGGCCTCAGT AGAGAGGTCA 
    TCGCAAGATT TTGTTGAAGA CTCACTAGAA CACAGTCAGT CACATGATAT CGCAGAGTGT 
    ATTCTGTCGC AAAATGTAAT GGAACACAGC AAATCACAAG TCTTAGATGA TGACAGGCCA 
    TCACAAGTTT ATGTTGGAGA CAACCAATCA GATAAATCTG TAGAAGTCAA CATTTCACAA 
    GATTCAGAAG AAGACAGGTC ATTAACAGAT TTTGTAGTAC ACAGCCAATC AGAGAACTCA 
    GTAGAACACA GCCAGTCACA GGATTTAGTA GAACCTAGGC AATCACATGA TTTAATAGAA 
    CACTTCAAAT CACTAGACAA CACAGAGCAC AAGCAATCAG ACATGAGTTA TATAAACTGC 
    AGTCAATCAC AGGCCTCAGT AGAAATCTTG CAATCACACG AGTCTGTTGA ACACAGCCAA 
    TCAGAACAGT CAGTAAAACA CAGCCAGTCA CAAGACACTG ACAATTTTAG CCAGACACAG 
    GATTTAATTA AGCACAGCCA ATCACAGACT TTCATAAAAT GCAAACACAA CCAATCACTG 
    GATGGCGTAC AATATGATCA GTCAAAGATC TTAATAGAAC AAACCCAATC ACAGGATTCC 
    GTAAAGGACA ATCCATCCCA AGATCTTGTC AAACATAGCC AATCACAGGA CACAGTAGAA 
    CCGAGTCAAT CACAGGTCTC AGTAGAACAC ATCCAATCAC AGGATTCCCA AGACTGCAGC 
    CATTCACAGA ACTCAACAGT ACACAGCCAA TCACAAACTT CAGTGGGACA AAGCCAATCC 
    CAGGATTTGG TAGAACAAAG TCGATCACCA GCTGCAGCCG AAAACAGTCA ATCACAGACT 
    TCAGTGGAAC ACAGCCAATC ACTGGATTCA GTGGAACACA TGAAATCTCA GACTTCGGTG 
    GAACACAGGC AATCACAGGT TGAATTGATT AAGGCACATT CCATGAACAC CCGGTGGATG 
    GTGACTGAGT CAGACACAGT AGCGTTTTAT GACAAGGTGC GATTAAAACA AGACTGCATG 
    GATGTTTGCG CAGATTGTGA AGTCGTTGAA TCCTTGGGAG TTTTATCAAA ACATGCTGAC 
    AATTTGAACT TCGAAAATAA CGAAAGACTA AATTTCAAAA CACAGGACTT AAATGACACT 
    TCACCTGTTG TTTCTGAATA CGAGTGTTTC CCACATGTGC CTAATGCATC TTCTATTGGA 
    GTGCCAGATT CACTGCATCA GTGCACGATT AGCAATTCAC CCTCAGACCT GCAGGTTAAA 
    ACTCAGCAGA GTTGGCCAGT ATTACAAATA CACACCGAAA AAGACGATTA CTCAGTTAGT 
    AGGTATCACA TGGATGGTTA CTGTTTGCAG GACAGTGAGC AAACAGAGTT TTACAAAACC 
    TACCAGTGCC CTAACAGTAC CATTAATTTT AATACACAGG GTGAGTTCAC TGAAGCTCGG 
    AAGGTTGTAT CAACTGAAGG TGTATTTACT AGAGGTTGTA TCAACACCAA ACTTTTTGAT 
    GGCAAAAGTG TTGACATTGT AGGAGGACCG CAGAAGGACA GCAACAAGCA TCACGTTATG 
    AAAGGGAAAG AATGTACAAT AGTTCCTTCA ATAGAGCAGT CAGTAATTTA CTCTGACAGT 
    TCAGGATGCA TAGAATATAA AAGGCCCAAA ACGTTGAGCA TTGCTTGTCC TGACATGACG 
    ACTGCTGAAT CTGAAGGGTG TCCTGTAGAC AGTCCTGTTG AATCACTTTC TACACTGTTT 
    GAGATTCCAC ATGACAGATC TACAGTCAGC CCCACAGACA TTTGCTTTGA GTTTAGTGAT 
    GGCCCTCATA TGAACTGTAC ACAAGTAGCA CAGAGTGTTA TAGAATATTC AGATGCCCCC 
    AAACTATTTC ACCAGTTTGA CACCCTGTCT GACAAACAAA GAAGACCTAA TGAGTCAGCT 
    GAAGCAATTG CGTCGAATGT TGATATTTTG TCTGGTGCAC GTTCCGATCC TTTCACAAAT 
    GCACAGACTC CTTTTGATTA TTCCCAGGTG AACAGCAGGA ACTATACACA GAGCTCAGAC 
    ACAAATAATA ACATTAAAGC CATTACTTTA GAAGAGTGTG CCTCGGGAGT ACAAATGTCA 
    TTGCCTAGTT CATTGCAGAA ATACAGTAGG TTTACAAATA ATGCTGAATG CTTAGTCTCA 
    AAAAGGGGTG ATTACAAAAA CATTGATTCT TTTAGTGCTC GTAATACTCT GGCTACCCCC 
    TTCAAAGCAC CTGAAATGAA GACTGAAGTG AACAGCAGAG AAAATGTTGT GAAATCTAGC 
    AGTCACATAG ATGTTACACA GAGGAAGCTC TGTGATTGCT GCGGTGGAAA GGAGTGCCAG 
    CTGCAGACAG CGGGTGTATT TCAGTTCAAG GGCACAGACG CCATCAGTCA GCGCAGAACT 
    GCAGTGTTGC AAACCTTGAG TCCCCTCCAC ACAGATGACA GCACACATGA AGCGGTAAAA 
    GTAGACTCTA CAAGCTGTGA ATTTTCACAT ATGGTCAGTT TGCTTACACA CAAACATCCG 
    AATCTTGTCC AAGAAGACTT CGTATTACCA CTGACTGGAG CTCATAAGGC TGATGATACT 
    CATGTATTAG AAAACATGGA GAGAAAGCAT GAAACTTTCC CAATAAATGC TCTTAAAAGC 
    ACTGAATCAA TATGTGAAAC TTCTGATCCT CTTTTTAAGC AAACATTGCA ATTGCACAAC 
    TCGGAGGTTG AGGGCGCATT TGTTTCATGT TGTCATATTT TGCCCATCAC CAACTTAGAG 
    GCCATTTTAG AGACGGACCG TTCCCCGGAG AGCTCATTTG CAGTAGAAAA TGAACTGGAT 
    GAGCAACAGA GAGGAAGTCC TTTATCAGAA GGTGGATTAA ATGAGGACCT AAGCTCTTGT 
    GAGGATGTCA AAAACCGCCC ACATGACAGC AGTTGTGATA TGCATACAAA AGCCAATGTA 
    GCCAATTTCA TGAATGAACC CGATGCTGGA GAAACAATGA ACTCTTTATC AAATGGGTTT 
    GACAGCAGTG ATGACCATAG TGTTTCCAGT TTGGTGTCAC AAGACCTTGA CTCCAGTACC 
    AACAGTGAGG AGACGGAAGA CACCATGCAG CCATCGTTTG GTCTTCGTGG TAATGCGAGA 
    AATAAAAGAA CACAGTCAGC CAAGGGCTCT ATGTTTTCTG GTTTGATTCG AATGCCCACC 
    TTCCGCAAGC CAAAACGAGA ACTCAAACGC AATAAAAACA GGGTTGATCC GAAAGTTAGG 
    GCATCCAGTG GGGATGGAGA TGAACAAGAT GAGATGAAGA ACAACAGGTC ACCCACATGC 
    AGCAAATCTC ACTCTTCACT GTACAAGACC CCTATATCTC AAAGTGCAGA TCACCTTACT 
    GATATTACAA AGCATTCTGA CCACTCAAAT GATGATGTTT TTGAAAGGGC TTTTGCTTTA 
    AATGTGCAAA ATAGAGAGAA ACACATGCAA CCTCAACACC CGTTTGAGCA GCAGCAGCAG 
    AAAGATGGAG ATGCACTCAG TTTTAGAGCT GGCCAGACAA CAGAGGGGTT GCAACAAAAA 
    AGATGCAAGA GCATTGATAA CTTAAACTTA CGCATGAAGC TGGCATTGGC TCACAAGTCT 
    ATTTCTAGCC TGTTTGAGAC CAGATATCCT GAGAGGGACC AAGAGCAAAT CTTACAGTTA 
    GAGAATGAGG ATGGGAAAAC CAAGCAATCA TGGAGAAAGA CCAAACGATG TAACGAAGCA 
    GAACTTTGCA AAAGAACTCC TGTTCCTGGG ACGGTCTGTG ACAAGTGCAT ACATCAGAGC 
    CAAATTGATT GTGCCTTCTG TGCTTATCAG AACCGGTCCA TATTAACCAA CTTACCAAAT 
    GTCCAAGCAA GATTGAAGAA CACGTGCCAT TCTGACCCGC AGAGTTTGAA ATCTGCACCT 
    CAGGAAGGGT TTGAAGATGG TAAATATCTG GAGGAAGGTG AGCAGCCTTG TGGTGCTTTC 
    TCAGATTCAG ACCAAGCATC GGTAGATGGT TTTGAGTCCA GTGTAGAGGA CAGGTCGCTG 
    TCACCTGCAC ACCCCACTGT TCTTGCACTT TCAAACCAAA TGTCACCTAC CTGGACAAGG 
    TCCCTAAGCT GCTTCGAGAC AACTGATACA CCGACAAGGC CCATGAGCCC CAAACCACAC 
    AGTCCTGGTC TGGGCTGGAC ACAGCGGAGG AGCTTCCGCT ACCCCTCAAG ATCTGTTGCA 
    TCTTCTCTCT GCTTGCTTGG TCAGGGAGTG AGCACTGACG GTCTCTTAGA CCCACCTCAG 
    AGGCCGACAT CTCTAAAACC AAGGACAACA CAACTCACCA CAGCTCACTC CTTCGATTCT 
    GAGTACTTAC TGGAGGACAG CAGTTCAGAC AACCAGTCTC AGAATAGCCT GAATTCAGCC 
    TCCCTGATCA GCAAAACAGA GCATGTACAG CACATTACTG AAGCAGAGAG AAGACCACAA 
    GAAAATGCAT GTGTGCTTCG GGTGAAGTCC AGAGATCCAG GTACAGCTGC GCCCAGGCCT 
    ATGTCAGACC TGTACGGCTG GACCATGTCT CTTCAGGGCA CCAGAGAGTT GGCGCTCGAC 
    TTGGAGAGGA AGACTAACGC ACATCAGCGC TCATGCTCAT ATGAAACATT GACCGATGTG 
    GATAACATCC GCAAAAAGAA GCTAAGCAAG CAGCAAAGTT TGCGGCAGAT TAGCACATCA 
    ACATCTGAGA GGCATGAGAA ATTGCGAGCT AGACTTTCCC TCACATCCCC GGAGCAGTTC 
    CCCTCTGTTC CTCTCAAAGA CCACTTCTTC TCTCAGAGCA CACCTGTTGG GCTGGACTAT 
    CTGGGCTGGG CTCACCGTGC ATCCTTTTCA GAAACGAACC TCAGCGCACC GGAAAAAGAG 
    GCACATGGAC AGGTCGCACC TCTCGCATTG GTGATAACTG ATGGGCCACA GGATAAGTCT 
    GGTCTTGCAG ATGAGGTGGG CAGTGAAGAT GATCTGTACA ATGAATTCCG CAGCTCCTCC 
    AACCGATTCG GACACCCAGG AGGAGGAGGA GAACAGCTGG CCATAAATGA GCTGATCAGT 
    GATGGCAGTG TGTGCGCGGA GGCTCTATGG GACCACGTCA CCATGGATGA CCAGGAGCTG 
    GGCTTTAAAG CTGGAGATGT CATTGAAGTA GTGGACGCTA CGAACAAGGA GTGGTGGTGG 
    GGCAGGGTGC TGGACAGCGA GGGCTGGTTC CCCGCCAGCT TTGTTCGGTT ACGGGTGAAC 
    CAAGATGAGC CCATGGAGGA ATACCTAGCC CATCTGGACG GGGCCTCAGA GGGGTGTGGG 
    GCCAGCATGG GGGGACCCTT GGGACCTGGA CTGCCATGCA AGGAGCAGAT GAGAGCCAAC 
    GTCATCAATG AGATCATGAG CACAGAGAGA GATTACATTA AACACCTGAA GGACATATGT 
    GAGGGCTATA TAAAACAATG TCGGAAGAGG ACAGATATGT TTACAGAAGA GCAACTGCGC 
    ACTATCTTTG GCAACATCGA CGAGCTCTAC CGATTCCAGA AGAAGTTCCT CAAAGCCTTG 
    GAGAAAAAAT TCAACAAAGA GCATCCTCAC CTCAGCGAGA TCGGCTCCTG CTTCTTAGAG 
    CAGCAAACAA ACTTCCAGAT CTATTCTGAG TATTGCAACA ACCACCCCAA TGCCTGCGTG 
    CAGCTCTCCA AACTGATGAA GATCAAGAAG TATGTGTTCT TCTTTGAGGC TTGTCGCCTG 
    CTCCAGAAGA TGATTGACAT TTCGTTGGAT GGATTCCTAC TTACTCCTGT TCAGAAGATC 
    TGCAAGTATC CTCTGCAGCT CGCTGAACTG CTCAAGTACA CCAACCCACA GCACAGAGAT 
    TATAAGGATG TGGAAGCTGC CTTAAACGCT ATGAAGAATG TGGCCAGACT GATCAATGAG 
    AGAAAGAGGC GTCTAGAAAA TATTGACAAA ATCGCTCAGT GGCAGAGCTC CATAGAAGAC 
    TGGGAGGGTG AAGACGTCTT GAGCAGGAGC TCTGATCTGA TCTTTTCTGG AGACCTGACC 
    AAGATCTCCC AGCCACAGGC TAAAGGCCAG CAGAGAATGT TCTTCCTCTT TGACCACCAA 
    CTGGTTTTCT GTAAGAAGGA TCTGCTTCGG AGGGATATCT TGTACTACAA GGGTCGGTTA 
    GACATGGATG AGATGGAGGT GGTGGATGTG GAGGATGGGA AGGATAAGGA CTTTAATGTG 
    AGTGTGAAGA ATGCCTTGAA ATTGTGTTCT CCTGGAGGAG AGGAGGTTCA TCTCCTGTGT 
    GCCAAGAAGC CCGAACAAAA GCAGCGCTGG CTGAGAGCCT TCACAGATGA GCGGGAGCAG 
    GTCCAGCATG ACCTCGAGAC AGGTTTTACG ATTACAGAGG TCCAGAAGAA GCAGGCAATG 
    TTGAATGCAA CTAAGAGTCA TCCAACAGGG AAGCCTAAAG CATTAGCTTT ACAGCACCTT 
    TTGGCACTCA GAGCTCCACT CCTTCACCCC AAAACCAAGT GCCTGCGTTT GTCCGAGCCT 
    CCAGGACCTC TGGTTCCCAG GGCCACCCTA ACTTCACCTC TCCTCCCTGA AGGGCCTCAG 
    CGCAGGCAGG GGCGGGGGGT TATGGTGCCC CCTGGGGTCC TGCGCAGGGC TATATCCTGG 
    GTCTCCTCTC AAAGTAGAGA GCCCATCGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_009298437.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296712.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa42946
    Clone ID Related Accession (Same CDS sequence) XM_009298437.2 , XM_009298437.3
    Accession Version XM_009298437.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT 
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA 
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG 
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC 
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT 
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA 
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA 
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG 
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC 
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296712.1
    CDS372..9638
    Translation

    Target ORF information:

    RefSeq Version XM_009298437.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298437.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT 
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA 
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG 
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC 
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT 
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA 
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA 
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG 
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC 
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_009298437.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296712.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa42946
    Clone ID Related Accession (Same CDS sequence) XM_009298437.2 , XM_009298437.3
    Accession Version XM_009298437.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298437.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT 
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA 
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG 
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC 
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT 
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA 
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA 
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG 
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC 
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296712.1
    CDS347..9613
    Translation

    Target ORF information:

    RefSeq Version XM_009298437.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298437.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT 
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA 
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG 
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC 
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT 
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA 
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA 
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG 
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC 
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_009298440.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296715.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa42947
    Clone ID Related Accession (Same CDS sequence) XM_009298440.2 , XM_009298440.3
    Accession Version XM_009298440.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298440.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT 
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA 
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG 
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC 
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT 
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCTGTGA CCCGGCCCTA CTATGACTTC 
    CTGCTGCGGC AGAAACACCC GACGCTGCCC ACCGCCCTGC CCCAGCAGCA GGTCATCATG 
    CTGGCCGAAC CCAAACGCAA GACCTCCAAT TTCTGGCACA ACATCGGACG CTTGACACCG 
    TTCAAAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296715.1
    CDS369..9560
    Translation

    Target ORF information:

    RefSeq Version XM_009298440.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298440.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT 
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA 
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG 
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC 
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT 
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCTGTGA CCCGGCCCTA CTATGACTTC 
    CTGCTGCGGC AGAAACACCC GACGCTGCCC ACCGCCCTGC CCCAGCAGCA GGTCATCATG 
    CTGGCCGAAC CCAAACGCAA GACCTCCAAT TTCTGGCACA ACATCGGACG CTTGACACCG 
    TTCAAAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_009298440.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296715.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa42947
    Clone ID Related Accession (Same CDS sequence) XM_009298440.2 , XM_009298440.3
    Accession Version XM_009298440.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT 
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA 
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG 
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC 
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT 
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCTGTGA CCCGGCCCTA CTATGACTTC 
    CTGCTGCGGC AGAAACACCC GACGCTGCCC ACCGCCCTGC CCCAGCAGCA GGTCATCATG 
    CTGGCCGAAC CCAAACGCAA GACCTCCAAT TTCTGGCACA ACATCGGACG CTTGACACCG 
    TTCAAAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296715.1
    CDS342..9533
    Translation

    Target ORF information:

    RefSeq Version XM_009298440.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298440.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGCATGT ACAGCACATT ACTGAAGCAG AGAGAAGACC ACAAGAAAAT 
    GCATGTGTGC TTCGGGTGAA GTCCAGAGAT CCAGGTACAG CTGCGCCCAG GCCTATGTCA 
    GACCTGTACG GCTGGACCAT GTCTCTTCAG GGCACCAGAG AGTTGGCGCT CGACTTGGAG 
    AGGAAGACTA ACGCACATCA GCGCTCATGC TCATATGAAA CATTGACCGA TGTGGATAAC 
    ATCCGCAAAA AGAAGCTAAG CAAGCAGCAA AGTTTGCGGC AGATTAGCAC ATCAACATCT 
    GAGAGGCATG AGAAATTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCTGTGA CCCGGCCCTA CTATGACTTC 
    CTGCTGCGGC AGAAACACCC GACGCTGCCC ACCGCCCTGC CCCAGCAGCA GGTCATCATG 
    CTGGCCGAAC CCAAACGCAA GACCTCCAAT TTCTGGCACA ACATCGGACG CTTGACACCG 
    TTCAAAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_009298441.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296716.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa42948
    Clone ID Related Accession (Same CDS sequence) XM_009298441.3 , XM_009298441.2
    Accession Version XM_009298441.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGCCAAACA GAGGCTGTGA CCACAGAGAC ATGGCACAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG 
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA 
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG 
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT 
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT 
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT 
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG 
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA 
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT 
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT 
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG 
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA 
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC 
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC 
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA 
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT 
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG 
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC 
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA 
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG 
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA 
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC 
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT 
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG 
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG 
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC 
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG 
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA 
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA 
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC 
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG 
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA 
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA 
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA 
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT 
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA 
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG 
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG 
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG 
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA 
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC 
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA 
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC 
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC 
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA 
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA 
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT 
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA 
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC 
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA 
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG 
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG 
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG 
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC 
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT 
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC 
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT 
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT 
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT 
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA 
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT 
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT 
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT 
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC 
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG 
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG 
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT 
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC 
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA 
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA 
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA 
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG 
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC 
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC 
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT 
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC 
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA 
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA 
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT 
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT 
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA 
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC 
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA 
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG 
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT 
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT 
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG 
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT 
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG 
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC 
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT 
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA 
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT 
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT 
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG 
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA 
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG 
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG 
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA 
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG 
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA 
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA 
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT 
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG 
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA 
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA 
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT 
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT 
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT 
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA 
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA 
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG 
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC 
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT 
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA 
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG 
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC 
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA 
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG 
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC 
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC 
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG 
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG 
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG 
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT 
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC 
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA 
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG 
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC 
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA 
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC 
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC 
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG 
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA 
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT 
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA 
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG 
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC 
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG 
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT 
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG 
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG 
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA 
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC 
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG 
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT 
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC 
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296716.1
    CDS242..9337
    Translation

    Target ORF information:

    RefSeq Version XM_009298441.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298441.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGCCAAACA GAGGCTGTGA CCACAGAGAC ATGGCACAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG 
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA 
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG 
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT 
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT 
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT 
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG 
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA 
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT 
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT 
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG 
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA 
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC 
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC 
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA 
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT 
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG 
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC 
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA 
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG 
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA 
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC 
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT 
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG 
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG 
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC 
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG 
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA 
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA 
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC 
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG 
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA 
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA 
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA 
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT 
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA 
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG 
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG 
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG 
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA 
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC 
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA 
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC 
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC 
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA 
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA 
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT 
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA 
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC 
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA 
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG 
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG 
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG 
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC 
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT 
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC 
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT 
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT 
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT 
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA 
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT 
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT 
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT 
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC 
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG 
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG 
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT 
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC 
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA 
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA 
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA 
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG 
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC 
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC 
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT 
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC 
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA 
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA 
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT 
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT 
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA 
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC 
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA 
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG 
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT 
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT 
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG 
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT 
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG 
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC 
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT 
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA 
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT 
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT 
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG 
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA 
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG 
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG 
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA 
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG 
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA 
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA 
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT 
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG 
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA 
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA 
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT 
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT 
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT 
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA 
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA 
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG 
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC 
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT 
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA 
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG 
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC 
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA 
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG 
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC 
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC 
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG 
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG 
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG 
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT 
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC 
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA 
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG 
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC 
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA 
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC 
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC 
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG 
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA 
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT 
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA 
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG 
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC 
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG 
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT 
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG 
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG 
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA 
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC 
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG 
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT 
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC 
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_009298441.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296716.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa42948
    Clone ID Related Accession (Same CDS sequence) XM_009298441.3 , XM_009298441.2
    Accession Version XM_009298441.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    9001
    9061
    ATGCCAAACA GAGGCTGTGA CCACAGAGAC ATGGCACAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG 
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA 
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG 
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT 
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT 
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT 
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG 
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA 
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT 
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT 
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG 
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA 
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC 
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC 
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA 
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT 
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG 
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC 
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA 
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG 
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA 
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC 
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT 
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG 
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG 
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC 
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG 
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA 
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA 
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC 
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG 
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA 
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA 
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA 
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT 
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA 
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG 
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG 
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG 
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA 
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC 
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA 
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC 
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC 
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA 
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA 
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT 
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA 
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC 
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA 
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG 
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG 
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG 
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC 
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT 
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC 
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT 
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT 
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT 
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA 
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT 
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT 
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT 
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC 
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG 
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG 
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT 
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC 
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA 
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA 
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA 
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG 
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC 
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC 
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT 
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC 
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA 
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA 
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT 
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT 
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA 
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC 
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA 
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG 
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT 
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT 
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG 
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT 
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG 
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC 
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT 
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA 
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT 
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT 
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG 
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA 
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG 
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG 
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA 
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG 
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA 
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA 
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT 
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG 
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA 
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA 
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT 
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT 
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT 
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA 
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA 
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG 
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC 
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT 
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA 
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG 
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC 
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA 
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG 
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC 
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC 
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG 
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG 
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG 
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT 
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC 
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA 
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG 
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC 
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA 
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC 
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC 
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG 
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA 
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT 
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA 
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG 
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC 
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG 
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT 
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG 
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG 
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA 
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC 
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG 
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT 
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC 
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296716.1
    CDS268..9363
    Translation

    Target ORF information:

    RefSeq Version XM_009298441.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298441.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGCCAAACA GAGGCTGTGA CCACAGAGAC ATGGCACAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG 
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA 
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG 
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT 
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT 
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT 
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG 
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA 
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT 
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT 
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG 
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA 
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC 
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC 
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA 
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT 
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG 
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC 
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA 
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG 
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA 
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC 
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT 
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG 
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG 
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC 
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG 
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA 
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA 
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC 
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG 
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA 
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA 
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA 
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT 
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA 
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG 
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG 
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG 
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA 
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC 
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA 
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC 
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC 
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA 
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA 
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT 
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA 
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC 
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA 
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG 
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG 
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG 
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC 
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT 
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC 
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT 
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT 
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT 
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA 
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT 
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT 
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT 
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC 
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG 
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG 
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT 
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC 
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA 
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA 
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA 
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG 
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC 
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC 
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT 
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC 
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA 
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA 
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT 
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT 
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA 
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC 
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA 
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG 
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT 
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT 
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG 
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT 
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG 
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC 
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT 
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA 
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT 
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT 
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG 
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA 
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG 
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG 
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA 
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG 
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA 
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA 
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT 
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG 
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA 
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA 
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT 
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT 
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT 
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA 
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA 
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG 
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC 
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT 
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA 
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG 
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC 
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA 
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG 
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC 
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC 
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG 
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG 
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG 
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT 
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC 
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA 
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG 
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC 
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA 
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC 
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC 
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG 
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA 
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT 
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA 
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG 
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC 
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG 
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT 
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG 
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG 
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA 
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC 
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG 
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT 
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC 
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_009298442.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296717.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa42949
    Clone ID Related Accession (Same CDS sequence) XM_009298442.2 , XM_009298442.3
    Accession Version XM_009298442.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTTGAGTG CTATATACTT CCTACGAAGC TTCTTTAAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG 
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA 
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG 
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT 
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT 
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT 
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG 
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA 
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT 
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT 
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG 
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA 
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC 
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC 
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA 
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT 
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG 
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC 
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA 
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG 
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA 
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC 
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT 
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG 
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG 
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC 
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG 
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA 
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA 
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC 
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG 
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA 
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA 
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA 
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT 
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA 
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG 
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG 
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG 
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA 
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC 
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA 
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC 
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC 
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA 
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA 
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT 
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA 
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC 
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA 
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG 
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG 
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG 
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC 
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT 
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC 
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT 
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT 
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT 
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA 
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT 
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT 
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT 
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC 
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG 
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG 
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT 
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC 
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA 
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA 
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA 
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG 
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC 
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC 
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT 
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC 
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA 
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA 
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT 
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT 
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA 
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC 
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA 
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG 
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT 
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT 
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG 
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT 
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG 
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC 
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT 
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA 
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT 
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT 
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG 
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA 
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG 
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG 
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA 
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG 
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA 
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA 
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT 
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG 
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA 
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA 
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT 
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT 
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT 
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA 
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA 
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG 
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC 
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT 
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA 
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG 
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC 
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA 
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG 
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC 
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC 
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG 
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG 
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG 
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT 
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC 
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA 
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG 
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC 
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA 
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC 
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC 
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG 
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA 
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT 
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA 
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG 
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC 
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG 
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT 
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG 
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG 
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA 
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC 
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG 
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT 
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC 
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296717.1
    CDS163..9258
    Translation

    Target ORF information:

    RefSeq Version XM_009298442.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298442.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTTGAGTG CTATATACTT CCTACGAAGC TTCTTTAAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG 
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA 
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG 
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT 
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT 
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT 
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG 
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA 
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT 
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT 
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG 
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA 
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC 
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC 
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA 
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT 
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG 
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC 
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA 
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG 
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA 
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC 
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT 
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG 
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG 
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC 
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG 
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA 
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA 
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC 
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG 
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA 
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA 
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA 
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT 
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA 
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG 
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG 
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG 
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA 
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC 
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA 
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC 
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC 
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA 
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA 
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT 
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA 
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC 
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA 
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG 
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG 
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG 
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC 
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT 
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC 
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT 
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT 
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT 
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA 
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT 
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT 
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT 
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC 
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG 
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG 
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT 
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC 
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA 
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA 
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA 
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG 
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC 
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC 
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT 
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC 
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA 
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA 
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT 
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT 
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA 
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC 
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA 
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG 
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT 
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT 
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG 
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT 
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG 
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC 
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT 
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA 
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT 
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT 
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG 
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA 
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG 
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG 
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA 
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG 
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA 
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA 
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT 
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG 
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA 
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA 
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT 
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT 
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT 
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA 
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA 
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG 
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC 
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT 
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA 
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG 
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC 
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA 
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG 
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC 
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC 
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG 
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG 
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG 
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT 
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC 
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA 
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG 
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC 
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA 
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC 
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC 
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG 
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA 
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT 
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA 
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG 
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC 
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG 
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT 
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG 
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG 
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA 
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC 
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG 
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT 
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC 
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_009298442.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296717.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa42949
    Clone ID Related Accession (Same CDS sequence) XM_009298442.2 , XM_009298442.3
    Accession Version XM_009298442.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTTGAGTG CTATATACTT CCTACGAAGC TTCTTTAAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG 
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA 
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG 
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT 
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT 
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT 
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG 
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA 
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT 
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT 
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG 
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA 
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC 
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC 
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA 
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT 
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG 
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC 
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA 
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG 
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA 
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC 
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT 
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG 
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG 
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC 
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG 
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA 
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA 
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC 
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG 
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA 
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA 
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA 
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT 
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA 
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG 
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG 
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG 
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA 
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC 
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA 
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC 
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC 
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA 
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA 
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT 
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA 
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC 
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA 
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG 
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG 
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG 
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC 
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT 
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC 
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT 
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT 
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT 
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA 
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT 
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT 
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT 
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC 
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG 
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG 
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT 
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC 
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA 
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA 
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA 
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG 
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC 
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC 
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT 
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC 
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA 
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA 
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT 
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT 
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA 
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC 
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA 
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG 
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT 
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT 
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG 
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT 
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG 
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC 
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT 
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA 
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT 
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT 
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG 
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA 
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG 
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG 
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA 
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG 
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA 
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA 
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT 
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG 
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA 
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA 
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT 
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT 
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT 
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA 
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA 
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG 
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC 
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT 
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA 
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG 
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC 
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA 
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG 
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC 
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC 
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG 
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG 
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG 
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT 
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC 
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA 
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG 
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC 
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA 
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC 
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC 
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG 
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA 
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT 
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA 
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG 
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC 
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG 
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT 
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG 
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG 
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA 
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC 
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG 
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT 
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC 
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296717.1
    CDS160..9255
    Translation

    Target ORF information:

    RefSeq Version XM_009298442.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298442.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTTGAGTG CTATATACTT CCTACGAAGC TTCTTTAAGG CCCCCGCTTT AGAGGACTCT 
    GGGAGTGAAG CAAGCTGTCT GGAGGATGGG GACTCCCAGC AGGAGGAATG TGGAGAGCAG 
    CAGGACCACC AGGAATGGTG TGATCCGGTT TCTGAAGCAG CACAGGATAC AAAAGAAGAA 
    TATTTCGACA CACACCCATG GCACAGTGAC ACTTTCTCTG ACTCTAGTCA TGAAACTAAG 
    TACTGCTGCA GGACCTCTGA GACTGGTGCT GTGGGTCATC TGGACTCACA CGTTTCATTT 
    GTTAAACCCA CACCGTCAGC TAGAGAGAGA GATCCCTCTT TAGCTAAACG CCATCATGCT 
    GTCTTTGACA GTCTCAGTGG GACAGTGGAT AGAGTGCAAT TGCAAACACA GACAGGAAAT 
    AACAAAACCA TGCTCATGGG AGTTGATGGC ACTAAACGAG AATGTGAGGA GGCTTTTATG 
    AATTTTCCAG CAGGACCTGG ACTTGATTCT TCTTCAACGA ATGACCTATC TCATGTAGCA 
    ACACCCCTAG GGGATCTAAA GGACTCTGTA GAACTAAAAA TATTCCCCAA GCCAAGTGCT 
    AAAGACTCAT TATTCACCAT AACAGATGGA TGTGATGAAG CCGAGTTAGG GGGCACCTTT 
    GTTTCAACAT CAAAATGGGA TCAAACAAGT GCTCAGTCAA AACATAGTAA GCCACACATG 
    CAGTTCATAA ACTCAGACAA GAAAGAGTTA CAGCAAAAGG ATAAAAGTAA TATGGAAATA 
    CCATTGATTC CAAATCCACC TGTTCTGAAA TGTGCAGAAG CTGAACACAG TGAGTTACAC 
    AGTCAACATC ATAAGCTAAA TGTTGATGAC ACTGAGATCA GTTTATCTCC ATCACAACAC 
    ATTTCTGTAT ATGAGTCTTT ATCATTTGAG AAGCCTGTTG AACAGAAAGT TTTATCATCA 
    GAACACTCAC TTCACTCAGT AGAGCACAGC CAACCACAGA CATCAGTAGA GCACAGCCAT 
    CCACAGATAT CAGTAGAGCA CAGCCATCCA CAGATATCAG TAGAGCACAG CCAACCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT ATCAGTAGAG 
    CACAGCCAAC CACAGATATC AGTAGAGCAC AGCCAACCAC AGATATCAGT AGAGCACAGC 
    CATCCACAGA TATCAGTAGA GCACAGCCAA CCACAGATAT CATTAGAGCA CAGCCATCCA 
    CAGATATCAG TAGAGCACAG CCATCCACAG ATATCAGTAG AGCACAGCCG AACACATATG 
    TCAGTAGAGC ACAGCCAACC ACAGATGTTA GTAGAGCACA ACCAACCACA GATGTCAGTA 
    GAGCACAACC AACCGCAGAT GTCAGTAGAC CACAGCCAAC CACAGATATC AGTAGACCAC 
    AACCAACCGC AGATGTCAGT AGACCACAGC CAACCACAGA TATCAGCAGA GCACAGGCAT 
    TCACAGATGT CAGTAGAGCA CAGCCAAGCA CAGATGTCAG TAGACCACAG TCAACCACAG 
    ATGTCAGTAG GCCACAGTCA ATCACAGATA TCAGTAGAGC ACAGCCAACC ACAGATATCA 
    GTAGAGCACA GCCAACCACA GATATCAGTA GAGCACAGCC AACCACAGAT GTCAGCAGAG 
    CACAGGCAAT CACAGATGTC AGTAGAGCAC AGCCAACCTC AGATGTTAGT AGAGCACAGC 
    CAATCACAGA TGTCAGTAGA GCACAGCCAA TCACAGATGT CAGTAGAGCA CAGCCAATCG 
    CAGATGTCAG TAGAGCACAG CCAACCACAG ATGTTAGTAG AGCACAGCCA ACCACAGTTA 
    TTTGTAGAGC ACAGCCAATC ACAGAACTCT GCTGATCACA GCCAGTCACA AGCTTCTTTA 
    AACTGCAGCC AACCACAACT CTCAGAAGAA TTCAGGCCAT CACAAGATTT TGTTGAACAC 
    AACCAATCAA ATAAAACTGT AGATCTCAAC CAGTCACAGG ATTCAGTAAA ACACAGTCAG 
    TTGCAGGATA CAATGGAATA CAGTAAAACA CAAGCCTCAG TAGAGGAGGT TCCAACCAAA 
    CACAGCCAGA CAGAGAATTT AGTAGAAGAC AGGCAATCAC AGTATTCAAT AGAACACAGA 
    AAATCACAGA CATTAGTAGA AGACAGTCCA ACACATGATT TTGTTGAACT TTGTCAATCA 
    GGGCACTTAG CAGAACACAG CCAGTCAGAT TGTTTGATAG AATGCAGTCA ATCTACGAAT 
    TCAGTTAAAC ACAGCCAATC ACAGGACACA GCAGAGCACA ACCAATCAGA AGCTTCTGTA 
    ATCTGCAGTC AATCAAAGAA TTCAATAGAA CACGGTCCAT CACAGGCCTC AGTAGAGAGG 
    TCATCGCAAG ATTTTGTTGA AGACTCACTA GAACACAGTC AGTCACATGA TATCGCAGAG 
    TGTATTCTGT CGCAAAATGT AATGGAACAC AGCAAATCAC AAGTCTTAGA TGATGACAGG 
    CCATCACAAG TTTATGTTGG AGACAACCAA TCAGATAAAT CTGTAGAAGT CAACATTTCA 
    CAAGATTCAG AAGAAGACAG GTCATTAACA GATTTTGTAG TACACAGCCA ATCAGAGAAC 
    TCAGTAGAAC ACAGCCAGTC ACAGGATTTA GTAGAACCTA GGCAATCACA TGATTTAATA 
    GAACACTTCA AATCACTAGA CAACACAGAG CACAAGCAAT CAGACATGAG TTATATAAAC 
    TGCAGTCAAT CACAGGCCTC AGTAGAAATC TTGCAATCAC ACGAGTCTGT TGAACACAGC 
    CAATCAGAAC AGTCAGTAAA ACACAGCCAG TCACAAGACA CTGACAATTT TAGCCAGACA 
    CAGGATTTAA TTAAGCACAG CCAATCACAG ACTTTCATAA AATGCAAACA CAACCAATCA 
    CTGGATGGCG TACAATATGA TCAGTCAAAG ATCTTAATAG AACAAACCCA ATCACAGGAT 
    TCCGTAAAGG ACAATCCATC CCAAGATCTT GTCAAACATA GCCAATCACA GGACACAGTA 
    GAACCGAGTC AATCACAGGT CTCAGTAGAA CACATCCAAT CACAGGATTC CCAAGACTGC 
    AGCCATTCAC AGAACTCAAC AGTACACAGC CAATCACAAA CTTCAGTGGG ACAAAGCCAA 
    TCCCAGGATT TGGTAGAACA AAGTCGATCA CCAGCTGCAG CCGAAAACAG TCAATCACAG 
    ACTTCAGTGG AACACAGCCA ATCACTGGAT TCAGTGGAAC ACATGAAATC TCAGACTTCG 
    GTGGAACACA GGCAATCACA GGTTGAATTG ATTAAGGCAC ATTCCATGAA CACCCGGTGG 
    ATGGTGACTG AGTCAGACAC AGTAGCGTTT TATGACAAGG TGCGATTAAA ACAAGACTGC 
    ATGGATGTTT GCGCAGATTG TGAAGTCGTT GAATCCTTGG GAGTTTTATC AAAACATGCT 
    GACAATTTGA ACTTCGAAAA TAACGAAAGA CTAAATTTCA AAACACAGGA CTTAAATGAC 
    ACTTCACCTG TTGTTTCTGA ATACGAGTGT TTCCCACATG TGCCTAATGC ATCTTCTATT 
    GGAGTGCCAG ATTCACTGCA TCAGTGCACG ATTAGCAATT CACCCTCAGA CCTGCAGGTT 
    AAAACTCAGC AGAGTTGGCC AGTATTACAA ATACACACCG AAAAAGACGA TTACTCAGTT 
    AGTAGGTATC ACATGGATGG TTACTGTTTG CAGGACAGTG AGCAAACAGA GTTTTACAAA 
    ACCTACCAGT GCCCTAACAG TACCATTAAT TTTAATACAC AGGGTGAGTT CACTGAAGCT 
    CGGAAGGTTG TATCAACTGA AGGTGTATTT ACTAGAGGTT GTATCAACAC CAAACTTTTT 
    GATGGCAAAA GTGTTGACAT TGTAGGAGGA CCGCAGAAGG ACAGCAACAA GCATCACGTT 
    ATGAAAGGGA AAGAATGTAC AATAGTTCCT TCAATAGAGC AGTCAGTAAT TTACTCTGAC 
    AGTTCAGGAT GCATAGAATA TAAAAGGCCC AAAACGTTGA GCATTGCTTG TCCTGACATG 
    ACGACTGCTG AATCTGAAGG GTGTCCTGTA GACAGTCCTG TTGAATCACT TTCTACACTG 
    TTTGAGATTC CACATGACAG ATCTACAGTC AGCCCCACAG ACATTTGCTT TGAGTTTAGT 
    GATGGCCCTC ATATGAACTG TACACAAGTA GCACAGAGTG TTATAGAATA TTCAGATGCC 
    CCCAAACTAT TTCACCAGTT TGACACCCTG TCTGACAAAC AAAGAAGACC TAATGAGTCA 
    GCTGAAGCAA TTGCGTCGAA TGTTGATATT TTGTCTGGTG CACGTTCCGA TCCTTTCACA 
    AATGCACAGA CTCCTTTTGA TTATTCCCAG GTGAACAGCA GGAACTATAC ACAGAGCTCA 
    GACACAAATA ATAACATTAA AGCCATTACT TTAGAAGAGT GTGCCTCGGG AGTACAAATG 
    TCATTGCCTA GTTCATTGCA GAAATACAGT AGGTTTACAA ATAATGCTGA ATGCTTAGTC 
    TCAAAAAGGG GTGATTACAA AAACATTGAT TCTTTTAGTG CTCGTAATAC TCTGGCTACC 
    CCCTTCAAAG CACCTGAAAT GAAGACTGAA GTGAACAGCA GAGAAAATGT TGTGAAATCT 
    AGCAGTCACA TAGATGTTAC ACAGAGGAAG CTCTGTGATT GCTGCGGTGG AAAGGAGTGC 
    CAGCTGCAGA CAGCGGGTGT ATTTCAGTTC AAGGGCACAG ACGCCATCAG TCAGCGCAGA 
    ACTGCAGTGT TGCAAACCTT GAGTCCCCTC CACACAGATG ACAGCACACA TGAAGCGGTA 
    AAAGTAGACT CTACAAGCTG TGAATTTTCA CATATGGTCA GTTTGCTTAC ACACAAACAT 
    CCGAATCTTG TCCAAGAAGA CTTCGTATTA CCACTGACTG GAGCTCATAA GGCTGATGAT 
    ACTCATGTAT TAGAAAACAT GGAGAGAAAG CATGAAACTT TCCCAATAAA TGCTCTTAAA 
    AGCACTGAAT CAATATGTGA AACTTCTGAT CCTCTTTTTA AGCAAACATT GCAATTGCAC 
    AACTCGGAGG TTGAGGGCGC ATTTGTTTCA TGTTGTCATA TTTTGCCCAT CACCAACTTA 
    GAGGCCATTT TAGAGACGGA CCGTTCCCCG GAGAGCTCAT TTGCAGTAGA AAATGAACTG 
    GATGAGCAAC AGAGAGGAAG TCCTTTATCA GAAGGTGGAT TAAATGAGGA CCTAAGCTCT 
    TGTGAGGATG TCAAAAACCG CCCACATGAC AGCAGTTGTG ATATGCATAC AAAAGCCAAT 
    GTAGCCAATT TCATGAATGA ACCCGATGCT GGAGAAACAA TGAACTCTTT ATCAAATGGG 
    TTTGACAGCA GTGATGACCA TAGTGTTTCC AGTTTGGTGT CACAAGACCT TGACTCCAGT 
    ACCAACAGTG AGGAGACGGA AGACACCATG CAGCCATCGT TTGGTCTTCG TGGTAATGCG 
    AGAAATAAAA GAACACAGTC AGCCAAGGGC TCTATGTTTT CTGGTTTGAT TCGAATGCCC 
    ACCTTCCGCA AGCCAAAACG AGAACTCAAA CGCAATAAAA ACAGGGTTGA TCCGAAAGTT 
    AGGGCATCCA GTGGGGATGG AGATGAACAA GATGAGATGA AGAACAACAG GTCACCCACA 
    TGCAGCAAAT CTCACTCTTC ACTGTACAAG ACCCCTATAT CTCAAAGTGC AGATCACCTT 
    ACTGATATTA CAAAGCATTC TGACCACTCA AATGATGATG TTTTTGAAAG GGCTTTTGCT 
    TTAAATGTGC AAAATAGAGA GAAACACATG CAACCTCAAC ACCCGTTTGA GCAGCAGCAG 
    CAGAAAGATG GAGATGCACT CAGTTTTAGA GCTGGCCAGA CAACAGAGGG GTTGCAACAA 
    AAAAGATGCA AGAGCATTGA TAACTTAAAC TTACGCATGA AGCTGGCATT GGCTCACAAG 
    TCTATTTCTA GCCTGTTTGA GACCAGATAT CCTGAGAGGG ACCAAGAGCA AATCTTACAG 
    TTAGAGAATG AGGATGGGAA AACCAAGCAA TCATGGAGAA AGACCAAACG ATGTAACGAA 
    GCAGAACTTT GCAAAAGAAC TCCTGTTCCT GGGACGGTCT GTGACAAGTG CATACATCAG 
    AGCCAAATTG ATTGTGCCTT CTGTGCTTAT CAGAACCGGT CCATATTAAC CAACTTACCA 
    AATGTCCAAG CAAGATTGAA GAACACGTGC CATTCTGACC CGCAGAGTTT GAAATCTGCA 
    CCTCAGGAAG GGTTTGAAGA TGGTAAATAT CTGGAGGAAG GTGAGCAGCC TTGTGGTGCT 
    TTCTCAGATT CAGACCAAGC ATCGGTAGAT GGTTTTGAGT CCAGTGTAGA GGACAGGTCG 
    CTGTCACCTG CACACCCCAC TGTTCTTGCA CTTTCAAACC AAATGTCACC TACCTGGACA 
    AGGTCCCTAA GCTGCTTCGA GACAACTGAT ACACCGACAA GGCCCATGAG CCCCAAACCA 
    CACAGTCCTG GTCTGGGCTG GACACAGCGG AGGAGCTTCC GCTACCCCTC AAGATCTGTT 
    GCATCTTCTC TCTGCTTGCT TGGTCAGGGA GTGAGCACTG ACGGTCTCTT AGACCCACCT 
    CAGAGGCCGA CATCTCTAAA ACCAAGGACA ACACAACTCA CCACAGCTCA CTCCTTCGAT 
    TCTGAGTACT TACTGGAGGA CAGCAGTTCA GACAACCAGT CTCAGAATAG CCTGAATTCA 
    GCCTCCCTGA TCAGCAAAAC AGAGCATGTA CAGCACATTA CTGAAGCAGA GAGAAGACCA 
    CAAGAAAATG CATGTGTGCT TCGGGTGAAG TCCAGAGATC CAGGTACAGC TGCGCCCAGG 
    CCTATGTCAG ACCTGTACGG CTGGACCATG TCTCTTCAGG GCACCAGAGA GTTGGCGCTC 
    GACTTGGAGA GGAAGACTAA CGCACATCAG CGCTCATGCT CATATGAAAC ATTGACCGAT 
    GTGGATAACA TCCGCAAAAA GAAGCTAAGC AAGCAGCAAA GTTTGCGGCA GATTAGCACA 
    TCAACATCTG AGAGGCATGA GAAATTGCGA GCTAGACTTT CCCTCACATC CCCGGAGCAG 
    TTCCCCTCTG TTCCTCTCAA AGACCACTTC TTCTCTCAGA GCACACCTGT TGGGCTGGAC 
    TATCTGGGCT GGGCTCACCG TGCATCCTTT TCAGAAACGA ACCTCAGCGC ACCGGAAAAA 
    GAGGCACATG GACAGGTCGC ACCTCTCGCA TTGGTGATAA CTGATGGGCC ACAGGATAAG 
    TCTGGTCTTG CAGATGAGGT GGGCAGTGAA GATGATCTGT ACAATGAATT CCGCAGCTCC 
    TCCAACCGAT TCGGACACCC AGGAGGAGGA GGAGAACAGC TGGCCATAAA TGAGCTGATC 
    AGTGATGGCA GTGTGTGCGC GGAGGCTCTA TGGGACCACG TCACCATGGA TGACCAGGAG 
    CTGGGCTTTA AAGCTGGAGA TGTCATTGAA GTAGTGGACG CTACGAACAA GGAGTGGTGG 
    TGGGGCAGGG TGCTGGACAG CGAGGGCTGG TTCCCCGCCA GCTTTGTTCG GTTACGGGTG 
    AACCAAGATG AGCCCATGGA GGAATACCTA GCCCATCTGG ACGGGGCCTC AGAGGGGTGT 
    GGGGCCAGCA TGGGGGGACC CTTGGGACCT GGACTGCCAT GCAAGGAGCA GATGAGAGCC 
    AACGTCATCA ATGAGATCAT GAGCACAGAG AGAGATTACA TTAAACACCT GAAGGACATA 
    TGTGAGGGCT ATATAAAACA ATGTCGGAAG AGGACAGATA TGTTTACAGA AGAGCAACTG 
    CGCACTATCT TTGGCAACAT CGACGAGCTC TACCGATTCC AGAAGAAGTT CCTCAAAGCC 
    TTGGAGAAAA AATTCAACAA AGAGCATCCT CACCTCAGCG AGATCGGCTC CTGCTTCTTA 
    GAGCAGCAAA CAAACTTCCA GATCTATTCT GAGTATTGCA ACAACCACCC CAATGCCTGC 
    GTGCAGCTCT CCAAACTGAT GAAGATCAAG AAGTATGTGT TCTTCTTTGA GGCTTGTCGC 
    CTGCTCCAGA AGATGATTGA CATTTCGTTG GATGGATTCC TACTTACTCC TGTTCAGAAG 
    ATCTGCAAGT ATCCTCTGCA GCTCGCTGAA CTGCTCAAGT ACACCAACCC ACAGCACAGA 
    GATTATAAGG ATGTGGAAGC TGCCTTAAAC GCTATGAAGA ATGTGGCCAG ACTGATCAAT 
    GAGAGAAAGA GGCGTCTAGA AAATATTGAC AAAATCGCTC AGTGGCAGAG CTCCATAGAA 
    GACTGGGAGG GTGAAGACGT CTTGAGCAGG AGCTCTGATC TGATCTTTTC TGGAGACCTG 
    ACCAAGATCT CCCAGCCACA GGCTAAAGGC CAGCAGAGAA TGTTCTTCCT CTTTGACCAC 
    CAACTGGTTT TCTGTAAGAA GGATCTGCTT CGGAGGGATA TCTTGTACTA CAAGGGTCGG 
    TTAGACATGG ATGAGATGGA GGTGGTGGAT GTGGAGGATG GGAAGGATAA GGACTTTAAT 
    GTGAGTGTGA AGAATGCCTT GAAATTGTGT TCTCCTGGAG GAGAGGAGGT TCATCTCCTG 
    TGTGCCAAGA AGCCCGAACA AAAGCAGCGC TGGCTGAGAG CCTTCACAGA TGAGCGGGAG 
    CAGGTCCAGC ATGACCTCGA GACAGGTTTT ACGATTACAG AGGTCCAGAA GAAGCAGGCA 
    ATGTTGAATG CAACTAAGAG TCATCCAACA GGGAAGCCTA AAGCATTAGC TTTACAGCAC 
    CTTTTGGCAC TCAGAGCTCC ACTCCTTCAC CCCAAAACCA AGTGCCTGCG TTTGTCCGAG 
    CCTCCAGGAC CTCTGGTTCC CAGGGCCACC CTAACTTCAC CTCTCCTCCC TGAAGGGCCT 
    CAGCGCAGGC AGGGGCGGGG GGTTATGGTG CCCCCTGGGG TCCTGCGCAG GGCTATATCC 
    TGGGTCTCCT CTCAAAGTAG AGAGCCCATC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_009298443.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296718.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa42950
    Clone ID Related Accession (Same CDS sequence) XM_009298443.2 , XM_009298443.3
    Accession Version XM_009298443.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8967bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298443.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGTTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA 
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA 
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG 
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC 
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296718.1
    CDS372..9338
    Translation

    Target ORF information:

    RefSeq Version XM_009298443.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298443.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGTTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA 
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA 
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG 
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC 
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    arhgef4 ( XM_009298443.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296718.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID ODa42950
    Clone ID Related Accession (Same CDS sequence) XM_009298443.2 , XM_009298443.3
    Accession Version XM_009298443.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8967bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298443.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGTTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA 
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA 
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG 
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC 
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296718.1
    CDS347..9313
    Translation

    Target ORF information:

    RefSeq Version XM_009298443.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 4 (arhgef4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298443.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGACGAGG CGGAGGCTGC AGACGGGAGC GGAGCGCGGG GCGGTGAACT CGAGCGTCCC 
    GCTGACGGAG CGCTGGATGA GGTGCAGATG CATCTCGCCT GCTTACCGGA GCGATACATG 
    AGGGCGAAGT TTTCCCTCTC CGCGTACATA TTTTGCTGGA CAGTTGTGAA GTTGTGTCAG 
    AGATTACGAC CGTCGCTGGC TCTTCCTCGG GCCCCCGCTT TAGAGGACTC TGGGAGTGAA 
    GCAAGCTGTC TGGAGGATGG GGACTCCCAG CAGGAGGAAT GTGGAGAGCA GCAGGACCAC 
    CAGGAATGGT GTGATCCGGT TTCTGAAGCA GCACAGGATA CAAAAGAAGA ATATTTCGAC 
    ACACACCCAT GGCACAGTGA CACTTTCTCT GACTCTAGTC ATGAAACTAA GTACTGCTGC 
    AGGACCTCTG AGACTGGTGC TGTGGGTCAT CTGGACTCAC ACGTTTCATT TGTTAAACCC 
    ACACCGTCAG CTAGAGAGAG AGATCCCTCT TTAGCTAAAC GCCATCATGC TGTCTTTGAC 
    AGTCTCAGTG GGACAGTGGA TAGAGTGCAA TTGCAAACAC AGACAGGAAA TAACAAAACC 
    ATGCTCATGG GAGTTGATGG CACTAAACGA GAATGTGAGG AGGCTTTTAT GAATTTTCCA 
    GCAGGACCTG GACTTGATTC TTCTTCAACG AATGACCTAT CTCATGTAGC AACACCCCTA 
    GGGGATCTAA AGGACTCTGT AGAACTAAAA ATATTCCCCA AGCCAAGTGC TAAAGACTCA 
    TTATTCACCA TAACAGATGG ATGTGATGAA GCCGAGTTAG GGGGCACCTT TGTTTCAACA 
    TCAAAATGGG ATCAAACAAG TGCTCAGTCA AAACATAGTA AGCCACACAT GCAGTTCATA 
    AACTCAGACA AGAAAGAGTT ACAGCAAAAG GATAAAAGTA ATATGGAAAT ACCATTGATT 
    CCAAATCCAC CTGTTCTGAA ATGTGCAGAA GCTGAACACA GTGAGTTACA CAGTCAACAT 
    CATAAGCTAA ATGTTGATGA CACTGAGATC AGTTTATCTC CATCACAACA CATTTCTGTA 
    TATGAGTCTT TATCATTTGA GAAGCCTGTT GAACAGAAAG TTTTATCATC AGAACACTCA 
    CTTCACTCAG TAGAGCACAG CCAACCACAG ACATCAGTAG AGCACAGCCA TCCACAGATA 
    TCAGTAGAGC ACAGCCATCC ACAGATATCA GTAGAGCACA GCCAACCACA GATATCAGTA 
    GAGCACAGCC AACCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TATCAGTAGA GCACAGCCAA 
    CCACAGATAT CAGTAGAGCA CAGCCAACCA CAGATATCAG TAGAGCACAG CCATCCACAG 
    ATATCAGTAG AGCACAGCCA ACCACAGATA TCATTAGAGC ACAGCCATCC ACAGATATCA 
    GTAGAGCACA GCCATCCACA GATATCAGTA GAGCACAGCC GAACACATAT GTCAGTAGAG 
    CACAGCCAAC CACAGATGTT AGTAGAGCAC AACCAACCAC AGATGTCAGT AGAGCACAAC 
    CAACCGCAGA TGTCAGTAGA CCACAGCCAA CCACAGATAT CAGTAGACCA CAACCAACCG 
    CAGATGTCAG TAGACCACAG CCAACCACAG ATATCAGCAG AGCACAGGCA TTCACAGATG 
    TCAGTAGAGC ACAGCCAAGC ACAGATGTCA GTAGACCACA GTCAACCACA GATGTCAGTA 
    GGCCACAGTC AATCACAGAT ATCAGTAGAG CACAGCCAAC CACAGATATC AGTAGAGCAC 
    AGCCAACCAC AGATATCAGT AGAGCACAGC CAACCACAGA TGTCAGCAGA GCACAGGCAA 
    TCACAGATGT CAGTAGAGCA CAGCCAACCT CAGATGTTAG TAGAGCACAG CCAATCACAG 
    ATGTCAGTAG AGCACAGCCA ATCACAGATG TCAGTAGAGC ACAGCCAATC GCAGATGTCA 
    GTAGAGCACA GCCAACCACA GATGTTAGTA GAGCACAGCC AACCACAGTT ATTTGTAGAG 
    CACAGCCAAT CACAGAACTC TGCTGATCAC AGCCAGTCAC AAGCTTCTTT AAACTGCAGC 
    CAACCACAAC TCTCAGAAGA ATTCAGGCCA TCACAAGATT TTGTTGAACA CAACCAATCA 
    AATAAAACTG TAGATCTCAA CCAGTCACAG GATTCAGTAA AACACAGTCA GTTGCAGGAT 
    ACAATGGAAT ACAGTAAAAC ACAAGCCTCA GTAGAGGAGG TTCCAACCAA ACACAGCCAG 
    ACAGAGAATT TAGTAGAAGA CAGGCAATCA CAGTATTCAA TAGAACACAG AAAATCACAG 
    ACATTAGTAG AAGACAGTCC AACACATGAT TTTGTTGAAC TTTGTCAATC AGGGCACTTA 
    GCAGAACACA GCCAGTCAGA TTGTTTGATA GAATGCAGTC AATCTACGAA TTCAGTTAAA 
    CACAGCCAAT CACAGGACAC AGCAGAGCAC AACCAATCAG AAGCTTCTGT AATCTGCAGT 
    CAATCAAAGA ATTCAATAGA ACACGGTCCA TCACAGGCCT CAGTAGAGAG GTCATCGCAA 
    GATTTTGTTG AAGACTCACT AGAACACAGT CAGTCACATG ATATCGCAGA GTGTATTCTG 
    TCGCAAAATG TAATGGAACA CAGCAAATCA CAAGTCTTAG ATGATGACAG GCCATCACAA 
    GTTTATGTTG GAGACAACCA ATCAGATAAA TCTGTAGAAG TCAACATTTC ACAAGATTCA 
    GAAGAAGACA GGTCATTAAC AGATTTTGTA GTACACAGCC AATCAGAGAA CTCAGTAGAA 
    CACAGCCAGT CACAGGATTT AGTAGAACCT AGGCAATCAC ATGATTTAAT AGAACACTTC 
    AAATCACTAG ACAACACAGA GCACAAGCAA TCAGACATGA GTTATATAAA CTGCAGTCAA 
    TCACAGGCCT CAGTAGAAAT CTTGCAATCA CACGAGTCTG TTGAACACAG CCAATCAGAA 
    CAGTCAGTAA AACACAGCCA GTCACAAGAC ACTGACAATT TTAGCCAGAC ACAGGATTTA 
    ATTAAGCACA GCCAATCACA GACTTTCATA AAATGCAAAC ACAACCAATC ACTGGATGGC 
    GTACAATATG ATCAGTCAAA GATCTTAATA GAACAAACCC AATCACAGGA TTCCGTAAAG 
    GACAATCCAT CCCAAGATCT TGTCAAACAT AGCCAATCAC AGGACACAGT AGAACCGAGT 
    CAATCACAGG TCTCAGTAGA ACACATCCAA TCACAGGATT CCCAAGACTG CAGCCATTCA 
    CAGAACTCAA CAGTACACAG CCAATCACAA ACTTCAGTGG GACAAAGCCA ATCCCAGGAT 
    TTGGTAGAAC AAAGTCGATC ACCAGCTGCA GCCGAAAACA GTCAATCACA GACTTCAGTG 
    GAACACAGCC AATCACTGGA TTCAGTGGAA CACATGAAAT CTCAGACTTC GGTGGAACAC 
    AGGCAATCAC AGGTTGAATT GATTAAGGCA CATTCCATGA ACACCCGGTG GATGGTGACT 
    GAGTCAGACA CAGTAGCGTT TTATGACAAG GTGCGATTAA AACAAGACTG CATGGATGTT 
    TGCGCAGATT GTGAAGTCGT TGAATCCTTG GGAGTTTTAT CAAAACATGC TGACAATTTG 
    AACTTCGAAA ATAACGAAAG ACTAAATTTC AAAACACAGG ACTTAAATGA CACTTCACCT 
    GTTGTTTCTG AATACGAGTG TTTCCCACAT GTGCCTAATG CATCTTCTAT TGGAGTGCCA 
    GATTCACTGC ATCAGTGCAC GATTAGCAAT TCACCCTCAG ACCTGCAGGT TAAAACTCAG 
    CAGAGTTGGC CAGTATTACA AATACACACC GAAAAAGACG ATTACTCAGT TAGTAGGTAT 
    CACATGGATG GTTACTGTTT GCAGGACAGT GAGCAAACAG AGTTTTACAA AACCTACCAG 
    TGCCCTAACA GTACCATTAA TTTTAATACA CAGGGTGAGT TCACTGAAGC TCGGAAGGTT 
    GTATCAACTG AAGGTGTATT TACTAGAGGT TGTATCAACA CCAAACTTTT TGATGGCAAA 
    AGTGTTGACA TTGTAGGAGG ACCGCAGAAG GACAGCAACA AGCATCACGT TATGAAAGGG 
    AAAGAATGTA CAATAGTTCC TTCAATAGAG CAGTCAGTAA TTTACTCTGA CAGTTCAGGA 
    TGCATAGAAT ATAAAAGGCC CAAAACGTTG AGCATTGCTT GTCCTGACAT GACGACTGCT 
    GAATCTGAAG GGTGTCCTGT AGACAGTCCT GTTGAATCAC TTTCTACACT GTTTGAGATT 
    CCACATGACA GATCTACAGT CAGCCCCACA GACATTTGCT TTGAGTTTAG TGATGGCCCT 
    CATATGAACT GTACACAAGT AGCACAGAGT GTTATAGAAT ATTCAGATGC CCCCAAACTA 
    TTTCACCAGT TTGACACCCT GTCTGACAAA CAAAGAAGAC CTAATGAGTC AGCTGAAGCA 
    ATTGCGTCGA ATGTTGATAT TTTGTCTGGT GCACGTTCCG ATCCTTTCAC AAATGCACAG 
    ACTCCTTTTG ATTATTCCCA GGTGAACAGC AGGAACTATA CACAGAGCTC AGACACAAAT 
    AATAACATTA AAGCCATTAC TTTAGAAGAG TGTGCCTCGG GAGTACAAAT GTCATTGCCT 
    AGTTCATTGC AGAAATACAG TAGGTTTACA AATAATGCTG AATGCTTAGT CTCAAAAAGG 
    GGTGATTACA AAAACATTGA TTCTTTTAGT GCTCGTAATA CTCTGGCTAC CCCCTTCAAA 
    GCACCTGAAA TGAAGACTGA AGTGAACAGC AGAGAAAATG TTGTGAAATC TAGCAGTCAC 
    ATAGATGTTA CACAGAGGAA GCTCTGTGAT TGCTGCGGTG GAAAGGAGTG CCAGCTGCAG 
    ACAGCGGGTG TATTTCAGTT CAAGGGCACA GACGCCATCA GTCAGCGCAG AACTGCAGTG 
    TTGCAAACCT TGAGTCCCCT CCACACAGAT GACAGCACAC ATGAAGCGGT AAAAGTAGAC 
    TCTACAAGCT GTGAATTTTC ACATATGGTC AGTTTGCTTA CACACAAACA TCCGAATCTT 
    GTCCAAGAAG ACTTCGTATT ACCACTGACT GGAGCTCATA AGGCTGATGA TACTCATGTA 
    TTAGAAAACA TGGAGAGAAA GCATGAAACT TTCCCAATAA ATGCTCTTAA AAGCACTGAA 
    TCAATATGTG AAACTTCTGA TCCTCTTTTT AAGCAAACAT TGCAATTGCA CAACTCGGAG 
    GTTGAGGGCG CATTTGTTTC ATGTTGTCAT ATTTTGCCCA TCACCAACTT AGAGGCCATT 
    TTAGAGACGG ACCGTTCCCC GGAGAGCTCA TTTGCAGTAG AAAATGAACT GGATGAGCAA 
    CAGAGAGGAA GTCCTTTATC AGAAGGTGGA TTAAATGAGG ACCTAAGCTC TTGTGAGGAT 
    GTCAAAAACC GCCCACATGA CAGCAGTTGT GATATGCATA CAAAAGCCAA TGTAGCCAAT 
    TTCATGAATG AACCCGATGC TGGAGAAACA ATGAACTCTT TATCAAATGG GTTTGACAGC 
    AGTGATGACC ATAGTGTTTC CAGTTTGGTG TCACAAGACC TTGACTCCAG TACCAACAGT 
    GAGGAGACGG AAGACACCAT GCAGCCATCG TTTGGTCTTC GTGGTAATGC GAGAAATAAA 
    AGAACACAGT CAGCCAAGGG CTCTATGTTT TCTGGTTTGA TTCGAATGCC CACCTTCCGC 
    AAGCCAAAAC GAGAACTCAA ACGCAATAAA AACAGGGTTG ATCCGAAAGT TAGGGCATCC 
    AGTGGGGATG GAGATGAACA AGATGAGATG AAGAACAACA GGTCACCCAC ATGCAGCAAA 
    TCTCACTCTT CACTGTACAA GACCCCTATA TCTCAAAGTG CAGATCACCT TACTGATATT 
    ACAAAGCATT CTGACCACTC AAATGATGAT GTTTTTGAAA GGGCTTTTGC TTTAAATGTG 
    CAAAATAGAG AGAAACACAT GCAACCTCAA CACCCGTTTG AGCAGCAGCA GCAGAAAGAT 
    GGAGATGCAC TCAGTTTTAG AGCTGGCCAG ACAACAGAGG GGTTGCAACA AAAAAGATGC 
    AAGAGCATTG ATAACTTAAA CTTACGCATG AAGCTGGCAT TGGCTCACAA GTCTATTTCT 
    AGCCTGTTTG AGACCAGATA TCCTGAGAGG GACCAAGAGC AAATCTTACA GTTAGAGAAT 
    GAGGATGGGA AAACCAAGCA ATCATGGAGA AAGACCAAAC GATGTAACGA AGCAGAACTT 
    TGCAAAAGAA CTCCTGTTCC TGGGACGGTC TGTGACAAGT GCATACATCA GAGCCAAATT 
    GATTGTGCCT TCTGTGCTTA TCAGAACCGG TCCATATTAA CCAACTTACC AAATGTCCAA 
    GCAAGATTGA AGAACACGTG CCATTCTGAC CCGCAGAGTT TGAAATCTGC ACCTCAGGAA 
    GGGTTTGAAG ATGGTAAATA TCTGGAGGAA GGTGAGCAGC CTTGTGGTGC TTTCTCAGAT 
    TCAGACCAAG CATCGGTAGA TGGTTTTGAG TCCAGTGTAG AGGACAGGTC GCTGTCACCT 
    GCACACCCCA CTGTTCTTGC ACTTTCAAAC CAAATGTCAC CTACCTGGAC AAGGTCCCTA 
    AGCTGCTTCG AGACAACTGA TACACCGACA AGGCCCATGA GCCCCAAACC ACACAGTCCT 
    GGTCTGGGCT GGACACAGCG GAGGAGCTTC CGCTACCCCT CAAGATCTGT TGCATCTTCT 
    CTCTGCTTGC TTGGTCAGGG AGTGAGCACT GACGGTCTCT TAGACCCACC TCAGAGGCCG 
    ACATCTCTAA AACCAAGGAC AACACAACTC ACCACAGCTC ACTCCTTCGA TTCTGAGTAC 
    TTACTGGAGG ACAGCAGTTC AGACAACCAG TCTCAGAATA GCCTGAATTC AGCCTCCCTG 
    ATCAGCAAAA CAGAGTTGCG AGCTAGACTT TCCCTCACAT CCCCGGAGCA GTTCCCCTCT 
    GTTCCTCTCA AAGACCACTT CTTCTCTCAG AGCACACCTG TTGGGCTGGA CTATCTGGGC 
    TGGGCTCACC GTGCATCCTT TTCAGAAACG AACCTCAGCG CACCGGAAAA AGAGGCACAT 
    GGACAGGTCG CACCTCTCGC ATTGGTGATA ACTGATGGGC CACAGGATAA GTCTGGTCTT 
    GCAGATGAGG TGGGCAGTGA AGATGATCTG TACAATGAAT TCCGCAGCTC CTCCAACCGA 
    TTCGGACACC CAGGAGGAGG AGGAGAACAG CTGGCCATAA ATGAGCTGAT CAGTGATGGC 
    AGTGTGTGCG CGGAGGCTCT ATGGGACCAC GTCACCATGG ATGACCAGGA GCTGGGCTTT 
    AAAGCTGGAG ATGTCATTGA AGTAGTGGAC GCTACGAACA AGGAGTGGTG GTGGGGCAGG 
    GTGCTGGACA GCGAGGGCTG GTTCCCCGCC AGCTTTGTTC GGTTACGGGT GAACCAAGAT 
    GAGCCCATGG AGGAATACCT AGCCCATCTG GACGGGGCCT CAGAGGGGTG TGGGGCCAGC 
    ATGGGGGGAC CCTTGGGACC TGGACTGCCA TGCAAGGAGC AGATGAGAGC CAACGTCATC 
    AATGAGATCA TGAGCACAGA GAGAGATTAC ATTAAACACC TGAAGGACAT ATGTGAGGGC 
    TATATAAAAC AATGTCGGAA GAGGACAGAT ATGTTTACAG AAGAGCAACT GCGCACTATC 
    TTTGGCAACA TCGACGAGCT CTACCGATTC CAGAAGAAGT TCCTCAAAGC CTTGGAGAAA 
    AAATTCAACA AAGAGCATCC TCACCTCAGC GAGATCGGCT CCTGCTTCTT AGAGCAGCAA 
    ACAAACTTCC AGATCTATTC TGAGTATTGC AACAACCACC CCAATGCCTG CGTGCAGCTC 
    TCCAAACTGA TGAAGATCAA GAAGTATGTG TTCTTCTTTG AGGCTTGTCG CCTGCTCCAG 
    AAGATGATTG ACATTTCGTT GGATGGATTC CTACTTACTC CTGTTCAGAA GATCTGCAAG 
    TATCCTCTGC AGCTCGCTGA ACTGCTCAAG TACACCAACC CACAGCACAG AGATTATAAG 
    GATGTGGAAG CTGCCTTAAA CGCTATGAAG AATGTGGCCA GACTGATCAA TGAGAGAAAG 
    AGGCGTCTAG AAAATATTGA CAAAATCGCT CAGTGGCAGA GCTCCATAGA AGACTGGGAG 
    GGTGAAGACG TCTTGAGCAG GAGCTCTGAT CTGATCTTTT CTGGAGACCT GACCAAGATC 
    TCCCAGCCAC AGGCTAAAGG CCAGCAGAGA ATGTTCTTCC TCTTTGACCA CCAACTGGTT 
    TTCTGTAAGA AGGATCTGCT TCGGAGGGAT ATCTTGTACT ACAAGGGTCG GTTAGACATG 
    GATGAGATGG AGGTGGTGGA TGTGGAGGAT GGGAAGGATA AGGACTTTAA TGTGAGTGTG 
    AAGAATGCCT TGAAATTGTG TTCTCCTGGA GGAGAGGAGG TTCATCTCCT GTGTGCCAAG 
    AAGCCCGAAC AAAAGCAGCG CTGGCTGAGA GCCTTCACAG ATGAGCGGGA GCAGGTCCAG 
    CATGACCTCG AGACAGGTTT TACGATTACA GAGGTCCAGA AGAAGCAGGC AATGTTGAAT 
    GCAACTAAGA GTCATCCAAC AGGGAAGCCT AAAGCATTAG CTTTACAGCA CCTTTTGGCA 
    CTCAGAGCTC CACTCCTTCA CCCCAAAACC AAGTGCCTGC GTTTGTCCGA GCCTCCAGGA 
    CCTCTGGTTC CCAGGGCCAC CCTAACTTCA CCTCTCCTCC CTGAAGGGCC TCAGCGCAGG 
    CAGGGGCGGG GGGTTATGGT GCCCCCTGGG GTCCTGCGCA GGGCTATATC CTGGGTCTCC 
    TCTCAAAGTA GAGAGCCCAT CGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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