rc3h1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rc3h1
Entrez Gene ID	100485305
Full Name	ring finger and CCCH-type domains 1
General protein information	Preferred Names ring finger and CCCH-type domains 1 Names roquin-1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002931435.4	XP_002931481.1	roquin-1 isoform X1
XM_004914022.3	XP_004914079.1	roquin-1 isoform X2
XM_004914023.3	XP_004914080.1	roquin-1 isoform X3
XM_004914024.3	XP_004914081.1	roquin-1 isoform X4
XM_004914024.4	XP_004914081.1	roquin-1 isoform X4
XM_002931435.5	XP_002931481.1	roquin-1 isoform X1
XM_004914022.4	XP_004914079.1	roquin-1 isoform X2
XM_004914023.4	XP_004914080.1	roquin-1 isoform X3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	RC3H1	XP_001234605.2
Homo sapiens (human)	RC3H1	NP_742068.1
Pan troglodytes (chimpanzee)	RC3H1	XP_001151649.2
	RC3H1	XP_001102746.1
Canis lupus familiaris (dog)	RC3H1	XP_537186.2
Mus musculus (house mouse)	Rc3h1	NP_001020123.1
Danio rerio (zebrafish)	si:dkey-60b12.1	NP_001108155.1
Xenopus tropicalis (tropical clawed frog)	rc3h1	XP_002931481.1

Gene Ontology

The following rc3h1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rc3h1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa09540	XM_002931435.5 Latest version!	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa09541	XM_004914022.4 Latest version!	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa09542	XM_004914023.4 Latest version!	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa09543	XM_004914024.4 Latest version!	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa09543	XM_004914024.3	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa09540	XM_002931435.4	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa09541	XM_004914022.3	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa09542	XM_004914023.3	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rc3h1 ( XM_002931435.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931481.1 Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa09540
Clone ID Related Accession (Same CDS sequence)	XM_002931435.4 , XM_002931435.5
Accession Version	XM_002931435.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roquin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931435.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGGAACC ATATATTCGT 
TTGCCTCCTC AGCACACAGC ACAGCCTCAC CCCAGCCTGG ATGAACTTCA TAGACGCCGT 
AAGGAGATCA TGCTTCAGCT AGAAGAAAGA AAAGTGATTT CCCCTCCTCC CTTTGCACCC 
TCACCTACTC TGCCTCACAG CTTCCATCCA GAAGAGTACC TTGATGAGGA ATTAAAAGTA 
CCTGGGAAAT TTAAAGGGAA CGATTACAGT CAGTATTCAC CCTGGTCCTG TGACACATTC 
GGCTCCTACA TTGGAACCAA AGACAACAAA TCCAAAGATG TTTCATCTGG TGGCAGCGTG 
GAAGTAGCTA ATGTTGACAG CAAATCCCTA CGAGACCAAC GCATTGAGCT TCAGCGAAGA 
GCTACAGAGC CTGGTGAGGA TGATCTGATT CCCTTTGGAG ACAGACCTAC CGTTTCTCGC 
TTTGGGGCCA TCTCCCGCAC ATCGAAGGCC CTATACCAAC CACCAGGCCC AATGCAGCAG 
GTCTTGGCAG CCCAAGGAGC TGTAAAATCT AGCATTACAG ATTACAGCTT GTACAGTCAC 
GGTGGCTTGA GTGCTGCATC ATATCCACCC CATCAAAACC TTTCTTCTCA GGGACAGTTA 
AGTGAGCGTG AAAGGATATC CAAGCCTTTA CCAACATCTG AAAGAGAACA GCTTAGGATG 
GAACTGCAGC AAGTTAACCA GCAGATCAGT CAGCAGACAC AAATGCGTGG CATGGAGGTA 
GCTACTAACC GCCTGCTGTT GCCAAGAGAG GCACCTTCAC TTGGAAACCA GCAGCCACCT 
CCACCACAAC CACCAGCCAA GTGGCCAAGT ATGATGTCCA GTGAACAGCT GAGTCTTGAA 
CTTCATCAAG TGGAACGGGA GATTGGGAAG AGAACACGTG AACTCAGCTT GGAAAGCCAT 
TCTTTGAGTA TGAAAAGCAA GGCAGATACC ATCAAAGTAG AAAATGGGCA ACTGGAGGCA 
CCGACAAGTG TTCTATCACA TGAACTCCCA CTAACATTCA GCAGTGAGGT GCCAAATGGA 
TCAGGTCTGA CACAGGACAT CTCCATGTTG TCTAGCAAGA CAGCATCTTT AACTCTTTCA 
GAAGATCCAC AGGCTGGAGG AGACAAGAGT GAGCCGCTGA GATCTGGAGT TAATTCCACT 
GCTGCTCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931481.1
CDS	290..3661
Translation	MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQVPEQQQITLCGGCGAEDTKHYEEARKCVEELALYLKPLSTARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLEDGLVAVKTVVHGLVDYIQNHSKKGTDQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPIGAPDEGPMDLPPRKPSGLPNGIVPGTQLISRSTDSGFESVLKQVKLDQLSSSAPGSPPELLDSVPKASMSALPVNAHPAASRVSSDMPPLPVSKPIQMVPRGSQIYAPPPADTFYQECRGGGPPFDAAPYAQGVYYPPQSSQCVSRFVRPPPAATEPAATYLDHYTPYLQDRVVSTQYGTPTQQYQTIVPTQYQPHYDNRRVYPSNAPPYQREEIVRASPVPLEMPPAAISPYVPESRERYQGIEGYYTHPGQIRPSYHREPYIRLPPQHTAQPHPSLDELHRRRKEIMLQLEERKVISPPPFAPSPTLPHSFHPEEYLDEELKVPGKFKGNDYSQYSPWSCDTFGSYIGTKDNKSKDVSSGGSVEVANVDSKSLRDQRIELQRRATEPGEDDLIPFGDRPTVSRFGAISRTSKALYQPPGPMQQVLAAQGAVKSSITDYSLYSHGGLSAASYPPHQNLSSQGQLSERERISKPLPTSEREQLRMELQQVNQQISQQTQMRGMEVATNRLLLPREAPSLGNQQPPPPQPPAKWPSMMSSEQLSLELHQVEREIGKRTRELSLESHSLSMKSKADTIKVENGQLEAPTSVLSHELPLTFSSEVPNGSGLTQDISMLSSKTASLTLSEDPQAGGDKSEPLRSGVNSTAAP

Target ORF information:

RefSeq Version	XM_002931435.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931435.5

ORF Insert Sequence:

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGGAACC ATATATTCGT 
TTGCCTCCTC AGCACACAGC ACAGCCTCAC CCCAGCCTGG ATGAACTTCA TAGACGCCGT 
AAGGAGATCA TGCTTCAGCT AGAAGAAAGA AAAGTGATTT CCCCTCCTCC CTTTGCACCC 
TCACCTACTC TGCCTCACAG CTTCCATCCA GAAGAGTACC TTGATGAGGA ATTAAAAGTA 
CCTGGGAAAT TTAAAGGGAA CGATTACAGT CAGTATTCAC CCTGGTCCTG TGACACATTC 
GGCTCCTACA TTGGAACCAA AGACAACAAA TCCAAAGATG TTTCATCTGG TGGCAGCGTG 
GAAGTAGCTA ATGTTGACAG CAAATCCCTA CGAGACCAAC GCATTGAGCT TCAGCGAAGA 
GCTACAGAGC CTGGTGAGGA TGATCTGATT CCCTTTGGAG ACAGACCTAC CGTTTCTCGC 
TTTGGGGCCA TCTCCCGCAC ATCGAAGGCC CTATACCAAC CACCAGGCCC AATGCAGCAG 
GTCTTGGCAG CCCAAGGAGC TGTAAAATCT AGCATTACAG ATTACAGCTT GTACAGTCAC 
GGTGGCTTGA GTGCTGCATC ATATCCACCC CATCAAAACC TTTCTTCTCA GGGACAGTTA 
AGTGAGCGTG AAAGGATATC CAAGCCTTTA CCAACATCTG AAAGAGAACA GCTTAGGATG 
GAACTGCAGC AAGTTAACCA GCAGATCAGT CAGCAGACAC AAATGCGTGG CATGGAGGTA 
GCTACTAACC GCCTGCTGTT GCCAAGAGAG GCACCTTCAC TTGGAAACCA GCAGCCACCT 
CCACCACAAC CACCAGCCAA GTGGCCAAGT ATGATGTCCA GTGAACAGCT GAGTCTTGAA 
CTTCATCAAG TGGAACGGGA GATTGGGAAG AGAACACGTG AACTCAGCTT GGAAAGCCAT 
TCTTTGAGTA TGAAAAGCAA GGCAGATACC ATCAAAGTAG AAAATGGGCA ACTGGAGGCA 
CCGACAAGTG TTCTATCACA TGAACTCCCA CTAACATTCA GCAGTGAGGT GCCAAATGGA 
TCAGGTCTGA CACAGGACAT CTCCATGTTG TCTAGCAAGA CAGCATCTTT AACTCTTTCA 
GAAGATCCAC AGGCTGGAGG AGACAAGAGT GAGCCGCTGA GATCTGGAGT TAATTCCACT 
GCTGCTCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rc3h1 ( XM_004914022.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914079.1 Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa09541
Clone ID Related Accession (Same CDS sequence)	XM_004914022.3 , XM_004914022.4
Accession Version	XM_004914022.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roquin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914022.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGGAACC ATATATTCGT 
TTGCCTCCTC AGCACACAGC ACAGCCTCAC CCCAGCCTGG ATGAACTTCA TAGACGCCGT 
AAGGAGATCA TGCTTCAGCT AGAAGAAAGA AAAGTGATTT CCCCTCCTCC CTTTGCACCC 
TCACCTACTC TGCCTCACAG CTTCCATCCA GAAGAGTACC TTGATGAGGA ATTAAAAGTA 
CCTGGGAAAT TTAAAGGGAA CGATTACAGT CAGTATTCAC CCTGGTCCTG TGACACATTC 
GGCTCCTACA TTGGAACCAA AGACAACAAA TCCAAAGATG TTTCATCTGG TGGCAGCGTG 
GAAGTAGCTA ATGTTGACAG CAAATCCCTA CGAGACCAAC GCATTGAGCT TCAGCGAAGA 
GCTACAGAGC CTGGTGAGGA TGATCTGATT CCCTTTGGAG ACAGACCTAC CGTTTCTCGC 
TTTGGGGCCA TCTCCCGCAC ATCGAAGGCC CTATACCAAC CACCAGGCCC AATGCAGCAG 
GTCTTGGCAG CCCAAGGAGC TGTAAAATCT AGCATTACAG ATTACAGCTT GTACAGTCAC 
GGTGGCTTGA GTGCTGCATC ATATCCACCC CATCAAAACC TTTCTTCTCA GGGACAGTTA 
AGTGAGCGTG AAAGGATATC CAAGCCTTTA CCAACATCTG AAAGAGAACA GCTTAGGATG 
GAACTGCAGC AAGTTAACCA GCAGATCAGT CAGCAGACAC AAATGCGTGG CATGGAGGTA 
GCTACTAACC GCCTGCTGTT GCCAAGAGAG GCACCTTCAC TTGGAAACCA GCAGCCACCT 
CCACCACAAC CACCAGCCAA GTGGCCAAGT ATGATGTCCA GTGAACAGCT GAGTCTTGAA 
CTTCATCAAG TGGAACGGGA GATTGGGAAG AGAACACGTG AACTCAGCTT GGAAAGCCAT 
TCTTTGAGTA TGAAAAGCAA GGCAGATACC ATCAAAGTAG AAAATGGGCA ACTGGAGGCA 
CCGACAAGTG TTCTATCACA TGAACTCCCA CTAACATTCA GTGAGGTGCC AAATGGATCA 
GGTCTGACAC AGGACATCTC CATGTTGTCT AGCAAGACAG CATCTTTAAC TCTTTCAGAA 
GATCCACAGG CTGGAGGAGA CAAGAGTGAG CCGCTGAGAT CTGGAGTTAA TTCCACTGCT 
GCTCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914079.1
CDS	291..3659
Translation	MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQVPEQQQITLCGGCGAEDTKHYEEARKCVEELALYLKPLSTARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLEDGLVAVKTVVHGLVDYIQNHSKKGTDQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPIGAPDEGPMDLPPRKPSGLPNGIVPGTQLISRSTDSGFESVLKQVKLDQLSSSAPGSPPELLDSVPKASMSALPVNAHPAASRVSSDMPPLPVSKPIQMVPRGSQIYAPPPADTFYQECRGGGPPFDAAPYAQGVYYPPQSSQCVSRFVRPPPAATEPAATYLDHYTPYLQDRVVSTQYGTPTQQYQTIVPTQYQPHYDNRRVYPSNAPPYQREEIVRASPVPLEMPPAAISPYVPESRERYQGIEGYYTHPGQIRPSYHREPYIRLPPQHTAQPHPSLDELHRRRKEIMLQLEERKVISPPPFAPSPTLPHSFHPEEYLDEELKVPGKFKGNDYSQYSPWSCDTFGSYIGTKDNKSKDVSSGGSVEVANVDSKSLRDQRIELQRRATEPGEDDLIPFGDRPTVSRFGAISRTSKALYQPPGPMQQVLAAQGAVKSSITDYSLYSHGGLSAASYPPHQNLSSQGQLSERERISKPLPTSEREQLRMELQQVNQQISQQTQMRGMEVATNRLLLPREAPSLGNQQPPPPQPPAKWPSMMSSEQLSLELHQVEREIGKRTRELSLESHSLSMKSKADTIKVENGQLEAPTSVLSHELPLTFSEVPNGSGLTQDISMLSSKTASLTLSEDPQAGGDKSEPLRSGVNSTAAP

Target ORF information:

RefSeq Version	XM_004914022.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914022.4

ORF Insert Sequence:

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGGAACC ATATATTCGT 
TTGCCTCCTC AGCACACAGC ACAGCCTCAC CCCAGCCTGG ATGAACTTCA TAGACGCCGT 
AAGGAGATCA TGCTTCAGCT AGAAGAAAGA AAAGTGATTT CCCCTCCTCC CTTTGCACCC 
TCACCTACTC TGCCTCACAG CTTCCATCCA GAAGAGTACC TTGATGAGGA ATTAAAAGTA 
CCTGGGAAAT TTAAAGGGAA CGATTACAGT CAGTATTCAC CCTGGTCCTG TGACACATTC 
GGCTCCTACA TTGGAACCAA AGACAACAAA TCCAAAGATG TTTCATCTGG TGGCAGCGTG 
GAAGTAGCTA ATGTTGACAG CAAATCCCTA CGAGACCAAC GCATTGAGCT TCAGCGAAGA 
GCTACAGAGC CTGGTGAGGA TGATCTGATT CCCTTTGGAG ACAGACCTAC CGTTTCTCGC 
TTTGGGGCCA TCTCCCGCAC ATCGAAGGCC CTATACCAAC CACCAGGCCC AATGCAGCAG 
GTCTTGGCAG CCCAAGGAGC TGTAAAATCT AGCATTACAG ATTACAGCTT GTACAGTCAC 
GGTGGCTTGA GTGCTGCATC ATATCCACCC CATCAAAACC TTTCTTCTCA GGGACAGTTA 
AGTGAGCGTG AAAGGATATC CAAGCCTTTA CCAACATCTG AAAGAGAACA GCTTAGGATG 
GAACTGCAGC AAGTTAACCA GCAGATCAGT CAGCAGACAC AAATGCGTGG CATGGAGGTA 
GCTACTAACC GCCTGCTGTT GCCAAGAGAG GCACCTTCAC TTGGAAACCA GCAGCCACCT 
CCACCACAAC CACCAGCCAA GTGGCCAAGT ATGATGTCCA GTGAACAGCT GAGTCTTGAA 
CTTCATCAAG TGGAACGGGA GATTGGGAAG AGAACACGTG AACTCAGCTT GGAAAGCCAT 
TCTTTGAGTA TGAAAAGCAA GGCAGATACC ATCAAAGTAG AAAATGGGCA ACTGGAGGCA 
CCGACAAGTG TTCTATCACA TGAACTCCCA CTAACATTCA GTGAGGTGCC AAATGGATCA 
GGTCTGACAC AGGACATCTC CATGTTGTCT AGCAAGACAG CATCTTTAAC TCTTTCAGAA 
GATCCACAGG CTGGAGGAGA CAAGAGTGAG CCGCTGAGAT CTGGAGTTAA TTCCACTGCT 
GCTCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rc3h1 ( XM_004914023.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914080.1 Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X3, mRNA.

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CloneID	OXa09542
Clone ID Related Accession (Same CDS sequence)	XM_004914023.3 , XM_004914023.4
Accession Version	XM_004914023.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roquin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914023.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGACCCC AGCCTCGTTT GCTCAGAGTG TCCAGGAACT GACCATTGCT 
TTGCAAAGGA CTGGAGACCC CGCAAATCTT AATAGACTTC GACCTCATTT GGAGTTGTTA 
GCGAACATAG ACCCAAGTCC AGATGCCCCT CCTCCAACAT GGGAGCAGTT AGAGGATGGT 
CTGGTGGCAG TGAAAACTGT AGTGCATGGA TTAGTGGATT ATATTCAGAA TCATAGCAAG 
AAGGGAACAG ACCAACAGCA GCCTCCACAG CACAGCAAGT ACAAAACATA CATGTGTAGA 
GATATGAAAC AGAGAGGAGG CTGTCCCCGT GGGGCAAGCT GCACCTTTGC CCATTCTCAA 
GAAGAGTTAG AAAAGTTTCG GAAAATGAAC AAACGTTTGG TTCCAAGACG GCCACTCAGT 
GCTTCGCTTG GTCAGTTGAA TGAGGTTGGA CTACCGATAG GAGCACCTGA TGAAGGTCCT 
ATGGACTTGC CACCCCGAAA GCCAAGTGGG CTTCCCAACG GCATTGTTCC GGGGACACAG 
CTCATCTCAA GGAGTACAGA CTCTGGCTTT GAGTCAGTGC TGAAACAAGT AAAATTGGAT 
CAATTGAGCA GCAGTGCACC TGGATCTCCT CCAGAACTGC TGGATTCTGT CCCCAAGGCA 
TCTATGTCTG CTTTGCCTGT CAATGCTCAT CCTGCTGCTT CGCGTGTATC CAGTGATATG 
CCACCATTGC CGGTTTCCAA GCCAATCCAA ATGGTGCCAC GTGGTTCACA AATATATGCC 
CCTCCTCCAG CAGATACGTT TTACCAAGAA TGTCGGGGTG GTGGACCTCC ATTTGATGCA 
GCTCCCTATG CACAAGGTGT TTATTACCCA CCTCAGTCCT CCCAGTGTGT TTCACGCTTT 
GTCAGACCTC CTCCTGCTGC CACTGAACCA GCCGCCACCT ACCTCGACCA CTATACTCCC 
TACTTACAGG ACAGAGTTGT CAGCACACAG TATGGCACTC CCACTCAGCA GTACCAGACA 
ATTGTACCCA CCCAGTACCA GCCACATTAT GACAACCGTC GTGTTTATCC CTCTAATGCT 
CCACCTTACC AAAGGGAGGA AATAGTTCGT GCAAGTCCGG TGCCTCTAGA AATGCCTCCT 
GCTGCTATCT CTCCCTATGT TCCTGAATCC AGAGAGAGAT ATCAGGGAAT TGAAGGTTAT 
TATACACATC CAGGACAGAT AAGGCCATCA TATCATAGGG AACCATATAT TCGTTTGCCT 
CCTCAGCACA CAGCACAGCC TCACCCCAGC CTGGATGAAC TTCATAGACG CCGTAAGGAG 
ATCATGCTTC AGCTAGAAGA AAGAAAAGTG ATTTCCCCTC CTCCCTTTGC ACCCTCACCT 
ACTCTGCCTC ACAGCTTCCA TCCAGAAGAG TACCTTGATG AGGAATTAAA AGTACCTGGG 
AAATTTAAAG GGAACGATTA CAGTCAGTAT TCACCCTGGT CCTGTGACAC ATTCGGCTCC 
TACATTGGAA CCAAAGACAA CAAATCCAAA GATGTTTCAT CTGGTGGCAG CGTGGAAGTA 
GCTAATGTTG ACAGCAAATC CCTACGAGAC CAACGCATTG AGCTTCAGCG AAGAGCTACA 
GAGCCTGGTG AGGATGATCT GATTCCCTTT GGAGACAGAC CTACCGTTTC TCGCTTTGGG 
GCCATCTCCC GCACATCGAA GGCCCTATAC CAACCACCAG GCCCAATGCA GCAGGTCTTG 
GCAGCCCAAG GAGCTGTAAA ATCTAGCATT ACAGATTACA GCTTGTACAG TCACGGTGGC 
TTGAGTGCTG CATCATATCC ACCCCATCAA AACCTTTCTT CTCAGGGACA GTTAAGTGAG 
CGTGAAAGGA TATCCAAGCC TTTACCAACA TCTGAAAGAG AACAGCTTAG GATGGAACTG 
CAGCAAGTTA ACCAGCAGAT CAGTCAGCAG ACACAAATGC GTGGCATGGA GGTAGCTACT 
AACCGCCTGC TGTTGCCAAG AGAGGCACCT TCACTTGGAA ACCAGCAGCC ACCTCCACCA 
CAACCACCAG CCAAGTGGCC AAGTATGATG TCCAGTGAAC AGCTGAGTCT TGAACTTCAT 
CAAGTGGAAC GGGAGATTGG GAAGAGAACA CGTGAACTCA GCTTGGAAAG CCATTCTTTG 
AGTATGAAAA GCAAGGCAGA TACCATCAAA GTAGAAAATG GGCAACTGGA GGCACCGACA 
AGTGTTCTAT CACATGAACT CCCACTAACA TTCAGCAGTG AGGTGCCAAA TGGATCAGGT 
CTGACACAGG ACATCTCCAT GTTGTCTAGC AAGACAGCAT CTTTAACTCT TTCAGAAGAT 
CCACAGGCTG GAGGAGACAA GAGTGAGCCG CTGAGATCTG GAGTTAATTC CACTGCTGCT 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914080.1
CDS	290..3655
Translation	MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQVPEQQQITLCGGCGAEDTKHYEEARKCVEELALYLKPLSTARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLEDGLVAVKTVVHGLVDYIQNHSKKGTDQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPIGAPDEGPMDLPPRKPSGLPNGIVPGTQLISRSTDSGFESVLKQVKLDQLSSSAPGSPPELLDSVPKASMSALPVNAHPAASRVSSDMPPLPVSKPIQMVPRGSQIYAPPPADTFYQECRGGGPPFDAAPYAQGVYYPPQSSQCVSRFVRPPPAATEPAATYLDHYTPYLQDRVVSTQYGTPTQQYQTIVPTQYQPHYDNRRVYPSNAPPYQREEIVRASPVPLEMPPAAISPYVPESRERYQGIEGYYTHPGQIRPSYHREPYIRLPPQHTAQPHPSLDELHRRRKEIMLQLEERKVISPPPFAPSPTLPHSFHPEEYLDEELKVPGKFKGNDYSQYSPWSCDTFGSYIGTKDNKSKDVSSGGSVEVANVDSKSLRDQRIELQRRATEPGEDDLIPFGDRPTVSRFGAISRTSKALYQPPGPMQQVLAAQGAVKSSITDYSLYSHGGLSAASYPPHQNLSSQGQLSERERISKPLPTSEREQLRMELQQVNQQISQQTQMRGMEVATNRLLLPREAPSLGNQQPPPPQPPAKWPSMMSSEQLSLELHQVEREIGKRTRELSLESHSLSMKSKADTIKVENGQLEAPTSVLSHELPLTFSSEVPNGSGLTQDISMLSSKTASLTLSEDPQAGGDKSEPLRSGVNSTAAP

Target ORF information:

RefSeq Version	XM_004914023.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914023.4

ORF Insert Sequence:

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGACCCC AGCCTCGTTT GCTCAGAGTG TCCAGGAACT GACCATTGCT 
TTGCAAAGGA CTGGAGACCC CGCAAATCTT AATAGACTTC GACCTCATTT GGAGTTGTTA 
GCGAACATAG ACCCAAGTCC AGATGCCCCT CCTCCAACAT GGGAGCAGTT AGAGGATGGT 
CTGGTGGCAG TGAAAACTGT AGTGCATGGA TTAGTGGATT ATATTCAGAA TCATAGCAAG 
AAGGGAACAG ACCAACAGCA GCCTCCACAG CACAGCAAGT ACAAAACATA CATGTGTAGA 
GATATGAAAC AGAGAGGAGG CTGTCCCCGT GGGGCAAGCT GCACCTTTGC CCATTCTCAA 
GAAGAGTTAG AAAAGTTTCG GAAAATGAAC AAACGTTTGG TTCCAAGACG GCCACTCAGT 
GCTTCGCTTG GTCAGTTGAA TGAGGTTGGA CTACCGATAG GAGCACCTGA TGAAGGTCCT 
ATGGACTTGC CACCCCGAAA GCCAAGTGGG CTTCCCAACG GCATTGTTCC GGGGACACAG 
CTCATCTCAA GGAGTACAGA CTCTGGCTTT GAGTCAGTGC TGAAACAAGT AAAATTGGAT 
CAATTGAGCA GCAGTGCACC TGGATCTCCT CCAGAACTGC TGGATTCTGT CCCCAAGGCA 
TCTATGTCTG CTTTGCCTGT CAATGCTCAT CCTGCTGCTT CGCGTGTATC CAGTGATATG 
CCACCATTGC CGGTTTCCAA GCCAATCCAA ATGGTGCCAC GTGGTTCACA AATATATGCC 
CCTCCTCCAG CAGATACGTT TTACCAAGAA TGTCGGGGTG GTGGACCTCC ATTTGATGCA 
GCTCCCTATG CACAAGGTGT TTATTACCCA CCTCAGTCCT CCCAGTGTGT TTCACGCTTT 
GTCAGACCTC CTCCTGCTGC CACTGAACCA GCCGCCACCT ACCTCGACCA CTATACTCCC 
TACTTACAGG ACAGAGTTGT CAGCACACAG TATGGCACTC CCACTCAGCA GTACCAGACA 
ATTGTACCCA CCCAGTACCA GCCACATTAT GACAACCGTC GTGTTTATCC CTCTAATGCT 
CCACCTTACC AAAGGGAGGA AATAGTTCGT GCAAGTCCGG TGCCTCTAGA AATGCCTCCT 
GCTGCTATCT CTCCCTATGT TCCTGAATCC AGAGAGAGAT ATCAGGGAAT TGAAGGTTAT 
TATACACATC CAGGACAGAT AAGGCCATCA TATCATAGGG AACCATATAT TCGTTTGCCT 
CCTCAGCACA CAGCACAGCC TCACCCCAGC CTGGATGAAC TTCATAGACG CCGTAAGGAG 
ATCATGCTTC AGCTAGAAGA AAGAAAAGTG ATTTCCCCTC CTCCCTTTGC ACCCTCACCT 
ACTCTGCCTC ACAGCTTCCA TCCAGAAGAG TACCTTGATG AGGAATTAAA AGTACCTGGG 
AAATTTAAAG GGAACGATTA CAGTCAGTAT TCACCCTGGT CCTGTGACAC ATTCGGCTCC 
TACATTGGAA CCAAAGACAA CAAATCCAAA GATGTTTCAT CTGGTGGCAG CGTGGAAGTA 
GCTAATGTTG ACAGCAAATC CCTACGAGAC CAACGCATTG AGCTTCAGCG AAGAGCTACA 
GAGCCTGGTG AGGATGATCT GATTCCCTTT GGAGACAGAC CTACCGTTTC TCGCTTTGGG 
GCCATCTCCC GCACATCGAA GGCCCTATAC CAACCACCAG GCCCAATGCA GCAGGTCTTG 
GCAGCCCAAG GAGCTGTAAA ATCTAGCATT ACAGATTACA GCTTGTACAG TCACGGTGGC 
TTGAGTGCTG CATCATATCC ACCCCATCAA AACCTTTCTT CTCAGGGACA GTTAAGTGAG 
CGTGAAAGGA TATCCAAGCC TTTACCAACA TCTGAAAGAG AACAGCTTAG GATGGAACTG 
CAGCAAGTTA ACCAGCAGAT CAGTCAGCAG ACACAAATGC GTGGCATGGA GGTAGCTACT 
AACCGCCTGC TGTTGCCAAG AGAGGCACCT TCACTTGGAA ACCAGCAGCC ACCTCCACCA 
CAACCACCAG CCAAGTGGCC AAGTATGATG TCCAGTGAAC AGCTGAGTCT TGAACTTCAT 
CAAGTGGAAC GGGAGATTGG GAAGAGAACA CGTGAACTCA GCTTGGAAAG CCATTCTTTG 
AGTATGAAAA GCAAGGCAGA TACCATCAAA GTAGAAAATG GGCAACTGGA GGCACCGACA 
AGTGTTCTAT CACATGAACT CCCACTAACA TTCAGCAGTG AGGTGCCAAA TGGATCAGGT 
CTGACACAGG ACATCTCCAT GTTGTCTAGC AAGACAGCAT CTTTAACTCT TTCAGAAGAT 
CCACAGGCTG GAGGAGACAA GAGTGAGCCG CTGAGATCTG GAGTTAATTC CACTGCTGCT 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rc3h1 ( XM_004914024.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914081.1 Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X4, mRNA.

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CloneID	OXa09543
Clone ID Related Accession (Same CDS sequence)	XM_004914024.3 , XM_004914024.4
Accession Version	XM_004914024.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roquin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914024.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGCACAC AGCACAGCCT 
CACCCCAGCC TGGATGAACT TCATAGACGC CGTAAGGAGA TCATGCTTCA GCTAGAAGAA 
AGAAAAGTGA TTTCCCCTCC TCCCTTTGCA CCCTCACCTA CTCTGCCTCA CAGCTTCCAT 
CCAGAAGAGT ACCTTGATGA GGAATTAAAA GTACCTGGGA AATTTAAAGG GAACGATTAC 
AGTCAGTATT CACCCTGGTC CTGTGACACA TTCGGCTCCT ACATTGGAAC CAAAGACAAC 
AAATCCAAAG ATGTTTCATC TGGTGGCAGC GTGGAAGTAG CTAATGTTGA CAGCAAATCC 
CTACGAGACC AACGCATTGA GCTTCAGCGA AGAGCTACAG AGCCTGGTGA GGATGATCTG 
ATTCCCTTTG GAGACAGACC TACCGTTTCT CGCTTTGGGG CCATCTCCCG CACATCGAAG 
GCCCTATACC AACCACCAGG CCCAATGCAG CAGGTCTTGG CAGCCCAAGG AGCTGTAAAA 
TCTAGCATTA CAGATTACAG CTTGTACAGT CACGGTGGCT TGAGTGCTGC ATCATATCCA 
CCCCATCAAA ACCTTTCTTC TCAGGGACAG TTAAGTGAGC GTGAAAGGAT ATCCAAGCCT 
TTACCAACAT CTGAAAGAGA ACAGCTTAGG ATGGAACTGC AGCAAGTTAA CCAGCAGATC 
AGTCAGCAGA CACAAATGCG TGGCATGGAG GTAGCTACTA ACCGCCTGCT GTTGCCAAGA 
GAGGCACCTT CACTTGGAAA CCAGCAGCCA CCTCCACCAC AACCACCAGC CAAGTGGCCA 
AGTATGATGT CCAGTGAACA GCTGAGTCTT GAACTTCATC AAGTGGAACG GGAGATTGGG 
AAGAGAACAC GTGAACTCAG CTTGGAAAGC CATTCTTTGA GTATGAAAAG CAAGGCAGAT 
ACCATCAAAG TAGAAAATGG GCAACTGGAG GCACCGACAA GTGTTCTATC ACATGAACTC 
CCACTAACAT TCAGCAGTGA GGTGCCAAAT GGATCAGGTC TGACACAGGA CATCTCCATG 
TTGTCTAGCA AGACAGCATC TTTAACTCTT TCAGAAGATC CACAGGCTGG AGGAGACAAG 
AGTGAGCCGC TGAGATCTGG AGTTAATTCC ACTGCTGCTC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914081.1
CDS	290..3634
Translation	MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQVPEQQQITLCGGCGAEDTKHYEEARKCVEELALYLKPLSTARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLEDGLVAVKTVVHGLVDYIQNHSKKGTDQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPIGAPDEGPMDLPPRKPSGLPNGIVPGTQLISRSTDSGFESVLKQVKLDQLSSSAPGSPPELLDSVPKASMSALPVNAHPAASRVSSDMPPLPVSKPIQMVPRGSQIYAPPPADTFYQECRGGGPPFDAAPYAQGVYYPPQSSQCVSRFVRPPPAATEPAATYLDHYTPYLQDRVVSTQYGTPTQQYQTIVPTQYQPHYDNRRVYPSNAPPYQREEIVRASPVPLEMPPAAISPYVPESRERYQGIEGYYTHPGQIRPSYHRHTAQPHPSLDELHRRRKEIMLQLEERKVISPPPFAPSPTLPHSFHPEEYLDEELKVPGKFKGNDYSQYSPWSCDTFGSYIGTKDNKSKDVSSGGSVEVANVDSKSLRDQRIELQRRATEPGEDDLIPFGDRPTVSRFGAISRTSKALYQPPGPMQQVLAAQGAVKSSITDYSLYSHGGLSAASYPPHQNLSSQGQLSERERISKPLPTSEREQLRMELQQVNQQISQQTQMRGMEVATNRLLLPREAPSLGNQQPPPPQPPAKWPSMMSSEQLSLELHQVEREIGKRTRELSLESHSLSMKSKADTIKVENGQLEAPTSVLSHELPLTFSSEVPNGSGLTQDISMLSSKTASLTLSEDPQAGGDKSEPLRSGVNSTAAP

Target ORF information:

RefSeq Version	XM_004914024.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914024.4

ORF Insert Sequence:

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGCACAC AGCACAGCCT 
CACCCCAGCC TGGATGAACT TCATAGACGC CGTAAGGAGA TCATGCTTCA GCTAGAAGAA 
AGAAAAGTGA TTTCCCCTCC TCCCTTTGCA CCCTCACCTA CTCTGCCTCA CAGCTTCCAT 
CCAGAAGAGT ACCTTGATGA GGAATTAAAA GTACCTGGGA AATTTAAAGG GAACGATTAC 
AGTCAGTATT CACCCTGGTC CTGTGACACA TTCGGCTCCT ACATTGGAAC CAAAGACAAC 
AAATCCAAAG ATGTTTCATC TGGTGGCAGC GTGGAAGTAG CTAATGTTGA CAGCAAATCC 
CTACGAGACC AACGCATTGA GCTTCAGCGA AGAGCTACAG AGCCTGGTGA GGATGATCTG 
ATTCCCTTTG GAGACAGACC TACCGTTTCT CGCTTTGGGG CCATCTCCCG CACATCGAAG 
GCCCTATACC AACCACCAGG CCCAATGCAG CAGGTCTTGG CAGCCCAAGG AGCTGTAAAA 
TCTAGCATTA CAGATTACAG CTTGTACAGT CACGGTGGCT TGAGTGCTGC ATCATATCCA 
CCCCATCAAA ACCTTTCTTC TCAGGGACAG TTAAGTGAGC GTGAAAGGAT ATCCAAGCCT 
TTACCAACAT CTGAAAGAGA ACAGCTTAGG ATGGAACTGC AGCAAGTTAA CCAGCAGATC 
AGTCAGCAGA CACAAATGCG TGGCATGGAG GTAGCTACTA ACCGCCTGCT GTTGCCAAGA 
GAGGCACCTT CACTTGGAAA CCAGCAGCCA CCTCCACCAC AACCACCAGC CAAGTGGCCA 
AGTATGATGT CCAGTGAACA GCTGAGTCTT GAACTTCATC AAGTGGAACG GGAGATTGGG 
AAGAGAACAC GTGAACTCAG CTTGGAAAGC CATTCTTTGA GTATGAAAAG CAAGGCAGAT 
ACCATCAAAG TAGAAAATGG GCAACTGGAG GCACCGACAA GTGTTCTATC ACATGAACTC 
CCACTAACAT TCAGCAGTGA GGTGCCAAAT GGATCAGGTC TGACACAGGA CATCTCCATG 
TTGTCTAGCA AGACAGCATC TTTAACTCTT TCAGAAGATC CACAGGCTGG AGGAGACAAG 
AGTGAGCCGC TGAGATCTGG AGTTAATTCC ACTGCTGCTC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rc3h1 ( XM_002931435.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931481.1 Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X1, mRNA.

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CloneID	OXa09540
Clone ID Related Accession (Same CDS sequence)	XM_002931435.4 , XM_002931435.5
Accession Version	XM_002931435.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roquin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931435.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGGAACC ATATATTCGT 
TTGCCTCCTC AGCACACAGC ACAGCCTCAC CCCAGCCTGG ATGAACTTCA TAGACGCCGT 
AAGGAGATCA TGCTTCAGCT AGAAGAAAGA AAAGTGATTT CCCCTCCTCC CTTTGCACCC 
TCACCTACTC TGCCTCACAG CTTCCATCCA GAAGAGTACC TTGATGAGGA ATTAAAAGTA 
CCTGGGAAAT TTAAAGGGAA CGATTACAGT CAGTATTCAC CCTGGTCCTG TGACACATTC 
GGCTCCTACA TTGGAACCAA AGACAACAAA TCCAAAGATG TTTCATCTGG TGGCAGCGTG 
GAAGTAGCTA ATGTTGACAG CAAATCCCTA CGAGACCAAC GCATTGAGCT TCAGCGAAGA 
GCTACAGAGC CTGGTGAGGA TGATCTGATT CCCTTTGGAG ACAGACCTAC CGTTTCTCGC 
TTTGGGGCCA TCTCCCGCAC ATCGAAGGCC CTATACCAAC CACCAGGCCC AATGCAGCAG 
GTCTTGGCAG CCCAAGGAGC TGTAAAATCT AGCATTACAG ATTACAGCTT GTACAGTCAC 
GGTGGCTTGA GTGCTGCATC ATATCCACCC CATCAAAACC TTTCTTCTCA GGGACAGTTA 
AGTGAGCGTG AAAGGATATC CAAGCCTTTA CCAACATCTG AAAGAGAACA GCTTAGGATG 
GAACTGCAGC AAGTTAACCA GCAGATCAGT CAGCAGACAC AAATGCGTGG CATGGAGGTA 
GCTACTAACC GCCTGCTGTT GCCAAGAGAG GCACCTTCAC TTGGAAACCA GCAGCCACCT 
CCACCACAAC CACCAGCCAA GTGGCCAAGT ATGATGTCCA GTGAACAGCT GAGTCTTGAA 
CTTCATCAAG TGGAACGGGA GATTGGGAAG AGAACACGTG AACTCAGCTT GGAAAGCCAT 
TCTTTGAGTA TGAAAAGCAA GGCAGATACC ATCAAAGTAG AAAATGGGCA ACTGGAGGCA 
CCGACAAGTG TTCTATCACA TGAACTCCCA CTAACATTCA GCAGTGAGGT GCCAAATGGA 
TCAGGTCTGA CACAGGACAT CTCCATGTTG TCTAGCAAGA CAGCATCTTT AACTCTTTCA 
GAAGATCCAC AGGCTGGAGG AGACAAGAGT GAGCCGCTGA GATCTGGAGT TAATTCCACT 
GCTGCTCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931481.1
CDS	294..3665
Translation	MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQVPEQQQITLCGGCGAEDTKHYEEARKCVEELALYLKPLSTARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLEDGLVAVKTVVHGLVDYIQNHSKKGTDQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPIGAPDEGPMDLPPRKPSGLPNGIVPGTQLISRSTDSGFESVLKQVKLDQLSSSAPGSPPELLDSVPKASMSALPVNAHPAASRVSSDMPPLPVSKPIQMVPRGSQIYAPPPADTFYQECRGGGPPFDAAPYAQGVYYPPQSSQCVSRFVRPPPAATEPAATYLDHYTPYLQDRVVSTQYGTPTQQYQTIVPTQYQPHYDNRRVYPSNAPPYQREEIVRASPVPLEMPPAAISPYVPESRERYQGIEGYYTHPGQIRPSYHREPYIRLPPQHTAQPHPSLDELHRRRKEIMLQLEERKVISPPPFAPSPTLPHSFHPEEYLDEELKVPGKFKGNDYSQYSPWSCDTFGSYIGTKDNKSKDVSSGGSVEVANVDSKSLRDQRIELQRRATEPGEDDLIPFGDRPTVSRFGAISRTSKALYQPPGPMQQVLAAQGAVKSSITDYSLYSHGGLSAASYPPHQNLSSQGQLSERERISKPLPTSEREQLRMELQQVNQQISQQTQMRGMEVATNRLLLPREAPSLGNQQPPPPQPPAKWPSMMSSEQLSLELHQVEREIGKRTRELSLESHSLSMKSKADTIKVENGQLEAPTSVLSHELPLTFSSEVPNGSGLTQDISMLSSKTASLTLSEDPQAGGDKSEPLRSGVNSTAAP

Target ORF information:

RefSeq Version	XM_002931435.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931435.4

ORF Insert Sequence:

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGGAACC ATATATTCGT 
TTGCCTCCTC AGCACACAGC ACAGCCTCAC CCCAGCCTGG ATGAACTTCA TAGACGCCGT 
AAGGAGATCA TGCTTCAGCT AGAAGAAAGA AAAGTGATTT CCCCTCCTCC CTTTGCACCC 
TCACCTACTC TGCCTCACAG CTTCCATCCA GAAGAGTACC TTGATGAGGA ATTAAAAGTA 
CCTGGGAAAT TTAAAGGGAA CGATTACAGT CAGTATTCAC CCTGGTCCTG TGACACATTC 
GGCTCCTACA TTGGAACCAA AGACAACAAA TCCAAAGATG TTTCATCTGG TGGCAGCGTG 
GAAGTAGCTA ATGTTGACAG CAAATCCCTA CGAGACCAAC GCATTGAGCT TCAGCGAAGA 
GCTACAGAGC CTGGTGAGGA TGATCTGATT CCCTTTGGAG ACAGACCTAC CGTTTCTCGC 
TTTGGGGCCA TCTCCCGCAC ATCGAAGGCC CTATACCAAC CACCAGGCCC AATGCAGCAG 
GTCTTGGCAG CCCAAGGAGC TGTAAAATCT AGCATTACAG ATTACAGCTT GTACAGTCAC 
GGTGGCTTGA GTGCTGCATC ATATCCACCC CATCAAAACC TTTCTTCTCA GGGACAGTTA 
AGTGAGCGTG AAAGGATATC CAAGCCTTTA CCAACATCTG AAAGAGAACA GCTTAGGATG 
GAACTGCAGC AAGTTAACCA GCAGATCAGT CAGCAGACAC AAATGCGTGG CATGGAGGTA 
GCTACTAACC GCCTGCTGTT GCCAAGAGAG GCACCTTCAC TTGGAAACCA GCAGCCACCT 
CCACCACAAC CACCAGCCAA GTGGCCAAGT ATGATGTCCA GTGAACAGCT GAGTCTTGAA 
CTTCATCAAG TGGAACGGGA GATTGGGAAG AGAACACGTG AACTCAGCTT GGAAAGCCAT 
TCTTTGAGTA TGAAAAGCAA GGCAGATACC ATCAAAGTAG AAAATGGGCA ACTGGAGGCA 
CCGACAAGTG TTCTATCACA TGAACTCCCA CTAACATTCA GCAGTGAGGT GCCAAATGGA 
TCAGGTCTGA CACAGGACAT CTCCATGTTG TCTAGCAAGA CAGCATCTTT AACTCTTTCA 
GAAGATCCAC AGGCTGGAGG AGACAAGAGT GAGCCGCTGA GATCTGGAGT TAATTCCACT 
GCTGCTCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rc3h1 ( XM_004914022.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914079.1 Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa09541
Clone ID Related Accession (Same CDS sequence)	XM_004914022.3 , XM_004914022.4
Accession Version	XM_004914022.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roquin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914022.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGGAACC ATATATTCGT 
TTGCCTCCTC AGCACACAGC ACAGCCTCAC CCCAGCCTGG ATGAACTTCA TAGACGCCGT 
AAGGAGATCA TGCTTCAGCT AGAAGAAAGA AAAGTGATTT CCCCTCCTCC CTTTGCACCC 
TCACCTACTC TGCCTCACAG CTTCCATCCA GAAGAGTACC TTGATGAGGA ATTAAAAGTA 
CCTGGGAAAT TTAAAGGGAA CGATTACAGT CAGTATTCAC CCTGGTCCTG TGACACATTC 
GGCTCCTACA TTGGAACCAA AGACAACAAA TCCAAAGATG TTTCATCTGG TGGCAGCGTG 
GAAGTAGCTA ATGTTGACAG CAAATCCCTA CGAGACCAAC GCATTGAGCT TCAGCGAAGA 
GCTACAGAGC CTGGTGAGGA TGATCTGATT CCCTTTGGAG ACAGACCTAC CGTTTCTCGC 
TTTGGGGCCA TCTCCCGCAC ATCGAAGGCC CTATACCAAC CACCAGGCCC AATGCAGCAG 
GTCTTGGCAG CCCAAGGAGC TGTAAAATCT AGCATTACAG ATTACAGCTT GTACAGTCAC 
GGTGGCTTGA GTGCTGCATC ATATCCACCC CATCAAAACC TTTCTTCTCA GGGACAGTTA 
AGTGAGCGTG AAAGGATATC CAAGCCTTTA CCAACATCTG AAAGAGAACA GCTTAGGATG 
GAACTGCAGC AAGTTAACCA GCAGATCAGT CAGCAGACAC AAATGCGTGG CATGGAGGTA 
GCTACTAACC GCCTGCTGTT GCCAAGAGAG GCACCTTCAC TTGGAAACCA GCAGCCACCT 
CCACCACAAC CACCAGCCAA GTGGCCAAGT ATGATGTCCA GTGAACAGCT GAGTCTTGAA 
CTTCATCAAG TGGAACGGGA GATTGGGAAG AGAACACGTG AACTCAGCTT GGAAAGCCAT 
TCTTTGAGTA TGAAAAGCAA GGCAGATACC ATCAAAGTAG AAAATGGGCA ACTGGAGGCA 
CCGACAAGTG TTCTATCACA TGAACTCCCA CTAACATTCA GTGAGGTGCC AAATGGATCA 
GGTCTGACAC AGGACATCTC CATGTTGTCT AGCAAGACAG CATCTTTAAC TCTTTCAGAA 
GATCCACAGG CTGGAGGAGA CAAGAGTGAG CCGCTGAGAT CTGGAGTTAA TTCCACTGCT 
GCTCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914079.1
CDS	295..3663
Translation	MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQVPEQQQITLCGGCGAEDTKHYEEARKCVEELALYLKPLSTARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLEDGLVAVKTVVHGLVDYIQNHSKKGTDQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPIGAPDEGPMDLPPRKPSGLPNGIVPGTQLISRSTDSGFESVLKQVKLDQLSSSAPGSPPELLDSVPKASMSALPVNAHPAASRVSSDMPPLPVSKPIQMVPRGSQIYAPPPADTFYQECRGGGPPFDAAPYAQGVYYPPQSSQCVSRFVRPPPAATEPAATYLDHYTPYLQDRVVSTQYGTPTQQYQTIVPTQYQPHYDNRRVYPSNAPPYQREEIVRASPVPLEMPPAAISPYVPESRERYQGIEGYYTHPGQIRPSYHREPYIRLPPQHTAQPHPSLDELHRRRKEIMLQLEERKVISPPPFAPSPTLPHSFHPEEYLDEELKVPGKFKGNDYSQYSPWSCDTFGSYIGTKDNKSKDVSSGGSVEVANVDSKSLRDQRIELQRRATEPGEDDLIPFGDRPTVSRFGAISRTSKALYQPPGPMQQVLAAQGAVKSSITDYSLYSHGGLSAASYPPHQNLSSQGQLSERERISKPLPTSEREQLRMELQQVNQQISQQTQMRGMEVATNRLLLPREAPSLGNQQPPPPQPPAKWPSMMSSEQLSLELHQVEREIGKRTRELSLESHSLSMKSKADTIKVENGQLEAPTSVLSHELPLTFSEVPNGSGLTQDISMLSSKTASLTLSEDPQAGGDKSEPLRSGVNSTAAP

Target ORF information:

RefSeq Version	XM_004914022.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914022.3

ORF Insert Sequence:

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGGAACC ATATATTCGT 
TTGCCTCCTC AGCACACAGC ACAGCCTCAC CCCAGCCTGG ATGAACTTCA TAGACGCCGT 
AAGGAGATCA TGCTTCAGCT AGAAGAAAGA AAAGTGATTT CCCCTCCTCC CTTTGCACCC 
TCACCTACTC TGCCTCACAG CTTCCATCCA GAAGAGTACC TTGATGAGGA ATTAAAAGTA 
CCTGGGAAAT TTAAAGGGAA CGATTACAGT CAGTATTCAC CCTGGTCCTG TGACACATTC 
GGCTCCTACA TTGGAACCAA AGACAACAAA TCCAAAGATG TTTCATCTGG TGGCAGCGTG 
GAAGTAGCTA ATGTTGACAG CAAATCCCTA CGAGACCAAC GCATTGAGCT TCAGCGAAGA 
GCTACAGAGC CTGGTGAGGA TGATCTGATT CCCTTTGGAG ACAGACCTAC CGTTTCTCGC 
TTTGGGGCCA TCTCCCGCAC ATCGAAGGCC CTATACCAAC CACCAGGCCC AATGCAGCAG 
GTCTTGGCAG CCCAAGGAGC TGTAAAATCT AGCATTACAG ATTACAGCTT GTACAGTCAC 
GGTGGCTTGA GTGCTGCATC ATATCCACCC CATCAAAACC TTTCTTCTCA GGGACAGTTA 
AGTGAGCGTG AAAGGATATC CAAGCCTTTA CCAACATCTG AAAGAGAACA GCTTAGGATG 
GAACTGCAGC AAGTTAACCA GCAGATCAGT CAGCAGACAC AAATGCGTGG CATGGAGGTA 
GCTACTAACC GCCTGCTGTT GCCAAGAGAG GCACCTTCAC TTGGAAACCA GCAGCCACCT 
CCACCACAAC CACCAGCCAA GTGGCCAAGT ATGATGTCCA GTGAACAGCT GAGTCTTGAA 
CTTCATCAAG TGGAACGGGA GATTGGGAAG AGAACACGTG AACTCAGCTT GGAAAGCCAT 
TCTTTGAGTA TGAAAAGCAA GGCAGATACC ATCAAAGTAG AAAATGGGCA ACTGGAGGCA 
CCGACAAGTG TTCTATCACA TGAACTCCCA CTAACATTCA GTGAGGTGCC AAATGGATCA 
GGTCTGACAC AGGACATCTC CATGTTGTCT AGCAAGACAG CATCTTTAAC TCTTTCAGAA 
GATCCACAGG CTGGAGGAGA CAAGAGTGAG CCGCTGAGAT CTGGAGTTAA TTCCACTGCT 
GCTCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rc3h1 ( XM_004914023.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914080.1 Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa09542
Clone ID Related Accession (Same CDS sequence)	XM_004914023.3 , XM_004914023.4
Accession Version	XM_004914023.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roquin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914023.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGACCCC AGCCTCGTTT GCTCAGAGTG TCCAGGAACT GACCATTGCT 
TTGCAAAGGA CTGGAGACCC CGCAAATCTT AATAGACTTC GACCTCATTT GGAGTTGTTA 
GCGAACATAG ACCCAAGTCC AGATGCCCCT CCTCCAACAT GGGAGCAGTT AGAGGATGGT 
CTGGTGGCAG TGAAAACTGT AGTGCATGGA TTAGTGGATT ATATTCAGAA TCATAGCAAG 
AAGGGAACAG ACCAACAGCA GCCTCCACAG CACAGCAAGT ACAAAACATA CATGTGTAGA 
GATATGAAAC AGAGAGGAGG CTGTCCCCGT GGGGCAAGCT GCACCTTTGC CCATTCTCAA 
GAAGAGTTAG AAAAGTTTCG GAAAATGAAC AAACGTTTGG TTCCAAGACG GCCACTCAGT 
GCTTCGCTTG GTCAGTTGAA TGAGGTTGGA CTACCGATAG GAGCACCTGA TGAAGGTCCT 
ATGGACTTGC CACCCCGAAA GCCAAGTGGG CTTCCCAACG GCATTGTTCC GGGGACACAG 
CTCATCTCAA GGAGTACAGA CTCTGGCTTT GAGTCAGTGC TGAAACAAGT AAAATTGGAT 
CAATTGAGCA GCAGTGCACC TGGATCTCCT CCAGAACTGC TGGATTCTGT CCCCAAGGCA 
TCTATGTCTG CTTTGCCTGT CAATGCTCAT CCTGCTGCTT CGCGTGTATC CAGTGATATG 
CCACCATTGC CGGTTTCCAA GCCAATCCAA ATGGTGCCAC GTGGTTCACA AATATATGCC 
CCTCCTCCAG CAGATACGTT TTACCAAGAA TGTCGGGGTG GTGGACCTCC ATTTGATGCA 
GCTCCCTATG CACAAGGTGT TTATTACCCA CCTCAGTCCT CCCAGTGTGT TTCACGCTTT 
GTCAGACCTC CTCCTGCTGC CACTGAACCA GCCGCCACCT ACCTCGACCA CTATACTCCC 
TACTTACAGG ACAGAGTTGT CAGCACACAG TATGGCACTC CCACTCAGCA GTACCAGACA 
ATTGTACCCA CCCAGTACCA GCCACATTAT GACAACCGTC GTGTTTATCC CTCTAATGCT 
CCACCTTACC AAAGGGAGGA AATAGTTCGT GCAAGTCCGG TGCCTCTAGA AATGCCTCCT 
GCTGCTATCT CTCCCTATGT TCCTGAATCC AGAGAGAGAT ATCAGGGAAT TGAAGGTTAT 
TATACACATC CAGGACAGAT AAGGCCATCA TATCATAGGG AACCATATAT TCGTTTGCCT 
CCTCAGCACA CAGCACAGCC TCACCCCAGC CTGGATGAAC TTCATAGACG CCGTAAGGAG 
ATCATGCTTC AGCTAGAAGA AAGAAAAGTG ATTTCCCCTC CTCCCTTTGC ACCCTCACCT 
ACTCTGCCTC ACAGCTTCCA TCCAGAAGAG TACCTTGATG AGGAATTAAA AGTACCTGGG 
AAATTTAAAG GGAACGATTA CAGTCAGTAT TCACCCTGGT CCTGTGACAC ATTCGGCTCC 
TACATTGGAA CCAAAGACAA CAAATCCAAA GATGTTTCAT CTGGTGGCAG CGTGGAAGTA 
GCTAATGTTG ACAGCAAATC CCTACGAGAC CAACGCATTG AGCTTCAGCG AAGAGCTACA 
GAGCCTGGTG AGGATGATCT GATTCCCTTT GGAGACAGAC CTACCGTTTC TCGCTTTGGG 
GCCATCTCCC GCACATCGAA GGCCCTATAC CAACCACCAG GCCCAATGCA GCAGGTCTTG 
GCAGCCCAAG GAGCTGTAAA ATCTAGCATT ACAGATTACA GCTTGTACAG TCACGGTGGC 
TTGAGTGCTG CATCATATCC ACCCCATCAA AACCTTTCTT CTCAGGGACA GTTAAGTGAG 
CGTGAAAGGA TATCCAAGCC TTTACCAACA TCTGAAAGAG AACAGCTTAG GATGGAACTG 
CAGCAAGTTA ACCAGCAGAT CAGTCAGCAG ACACAAATGC GTGGCATGGA GGTAGCTACT 
AACCGCCTGC TGTTGCCAAG AGAGGCACCT TCACTTGGAA ACCAGCAGCC ACCTCCACCA 
CAACCACCAG CCAAGTGGCC AAGTATGATG TCCAGTGAAC AGCTGAGTCT TGAACTTCAT 
CAAGTGGAAC GGGAGATTGG GAAGAGAACA CGTGAACTCA GCTTGGAAAG CCATTCTTTG 
AGTATGAAAA GCAAGGCAGA TACCATCAAA GTAGAAAATG GGCAACTGGA GGCACCGACA 
AGTGTTCTAT CACATGAACT CCCACTAACA TTCAGCAGTG AGGTGCCAAA TGGATCAGGT 
CTGACACAGG ACATCTCCAT GTTGTCTAGC AAGACAGCAT CTTTAACTCT TTCAGAAGAT 
CCACAGGCTG GAGGAGACAA GAGTGAGCCG CTGAGATCTG GAGTTAATTC CACTGCTGCT 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914080.1
CDS	294..3659
Translation	MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQVPEQQQITLCGGCGAEDTKHYEEARKCVEELALYLKPLSTARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLEDGLVAVKTVVHGLVDYIQNHSKKGTDQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPIGAPDEGPMDLPPRKPSGLPNGIVPGTQLISRSTDSGFESVLKQVKLDQLSSSAPGSPPELLDSVPKASMSALPVNAHPAASRVSSDMPPLPVSKPIQMVPRGSQIYAPPPADTFYQECRGGGPPFDAAPYAQGVYYPPQSSQCVSRFVRPPPAATEPAATYLDHYTPYLQDRVVSTQYGTPTQQYQTIVPTQYQPHYDNRRVYPSNAPPYQREEIVRASPVPLEMPPAAISPYVPESRERYQGIEGYYTHPGQIRPSYHREPYIRLPPQHTAQPHPSLDELHRRRKEIMLQLEERKVISPPPFAPSPTLPHSFHPEEYLDEELKVPGKFKGNDYSQYSPWSCDTFGSYIGTKDNKSKDVSSGGSVEVANVDSKSLRDQRIELQRRATEPGEDDLIPFGDRPTVSRFGAISRTSKALYQPPGPMQQVLAAQGAVKSSITDYSLYSHGGLSAASYPPHQNLSSQGQLSERERISKPLPTSEREQLRMELQQVNQQISQQTQMRGMEVATNRLLLPREAPSLGNQQPPPPQPPAKWPSMMSSEQLSLELHQVEREIGKRTRELSLESHSLSMKSKADTIKVENGQLEAPTSVLSHELPLTFSSEVPNGSGLTQDISMLSSKTASLTLSEDPQAGGDKSEPLRSGVNSTAAP

Target ORF information:

RefSeq Version	XM_004914023.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914023.3

ORF Insert Sequence:

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGACCCC AGCCTCGTTT GCTCAGAGTG TCCAGGAACT GACCATTGCT 
TTGCAAAGGA CTGGAGACCC CGCAAATCTT AATAGACTTC GACCTCATTT GGAGTTGTTA 
GCGAACATAG ACCCAAGTCC AGATGCCCCT CCTCCAACAT GGGAGCAGTT AGAGGATGGT 
CTGGTGGCAG TGAAAACTGT AGTGCATGGA TTAGTGGATT ATATTCAGAA TCATAGCAAG 
AAGGGAACAG ACCAACAGCA GCCTCCACAG CACAGCAAGT ACAAAACATA CATGTGTAGA 
GATATGAAAC AGAGAGGAGG CTGTCCCCGT GGGGCAAGCT GCACCTTTGC CCATTCTCAA 
GAAGAGTTAG AAAAGTTTCG GAAAATGAAC AAACGTTTGG TTCCAAGACG GCCACTCAGT 
GCTTCGCTTG GTCAGTTGAA TGAGGTTGGA CTACCGATAG GAGCACCTGA TGAAGGTCCT 
ATGGACTTGC CACCCCGAAA GCCAAGTGGG CTTCCCAACG GCATTGTTCC GGGGACACAG 
CTCATCTCAA GGAGTACAGA CTCTGGCTTT GAGTCAGTGC TGAAACAAGT AAAATTGGAT 
CAATTGAGCA GCAGTGCACC TGGATCTCCT CCAGAACTGC TGGATTCTGT CCCCAAGGCA 
TCTATGTCTG CTTTGCCTGT CAATGCTCAT CCTGCTGCTT CGCGTGTATC CAGTGATATG 
CCACCATTGC CGGTTTCCAA GCCAATCCAA ATGGTGCCAC GTGGTTCACA AATATATGCC 
CCTCCTCCAG CAGATACGTT TTACCAAGAA TGTCGGGGTG GTGGACCTCC ATTTGATGCA 
GCTCCCTATG CACAAGGTGT TTATTACCCA CCTCAGTCCT CCCAGTGTGT TTCACGCTTT 
GTCAGACCTC CTCCTGCTGC CACTGAACCA GCCGCCACCT ACCTCGACCA CTATACTCCC 
TACTTACAGG ACAGAGTTGT CAGCACACAG TATGGCACTC CCACTCAGCA GTACCAGACA 
ATTGTACCCA CCCAGTACCA GCCACATTAT GACAACCGTC GTGTTTATCC CTCTAATGCT 
CCACCTTACC AAAGGGAGGA AATAGTTCGT GCAAGTCCGG TGCCTCTAGA AATGCCTCCT 
GCTGCTATCT CTCCCTATGT TCCTGAATCC AGAGAGAGAT ATCAGGGAAT TGAAGGTTAT 
TATACACATC CAGGACAGAT AAGGCCATCA TATCATAGGG AACCATATAT TCGTTTGCCT 
CCTCAGCACA CAGCACAGCC TCACCCCAGC CTGGATGAAC TTCATAGACG CCGTAAGGAG 
ATCATGCTTC AGCTAGAAGA AAGAAAAGTG ATTTCCCCTC CTCCCTTTGC ACCCTCACCT 
ACTCTGCCTC ACAGCTTCCA TCCAGAAGAG TACCTTGATG AGGAATTAAA AGTACCTGGG 
AAATTTAAAG GGAACGATTA CAGTCAGTAT TCACCCTGGT CCTGTGACAC ATTCGGCTCC 
TACATTGGAA CCAAAGACAA CAAATCCAAA GATGTTTCAT CTGGTGGCAG CGTGGAAGTA 
GCTAATGTTG ACAGCAAATC CCTACGAGAC CAACGCATTG AGCTTCAGCG AAGAGCTACA 
GAGCCTGGTG AGGATGATCT GATTCCCTTT GGAGACAGAC CTACCGTTTC TCGCTTTGGG 
GCCATCTCCC GCACATCGAA GGCCCTATAC CAACCACCAG GCCCAATGCA GCAGGTCTTG 
GCAGCCCAAG GAGCTGTAAA ATCTAGCATT ACAGATTACA GCTTGTACAG TCACGGTGGC 
TTGAGTGCTG CATCATATCC ACCCCATCAA AACCTTTCTT CTCAGGGACA GTTAAGTGAG 
CGTGAAAGGA TATCCAAGCC TTTACCAACA TCTGAAAGAG AACAGCTTAG GATGGAACTG 
CAGCAAGTTA ACCAGCAGAT CAGTCAGCAG ACACAAATGC GTGGCATGGA GGTAGCTACT 
AACCGCCTGC TGTTGCCAAG AGAGGCACCT TCACTTGGAA ACCAGCAGCC ACCTCCACCA 
CAACCACCAG CCAAGTGGCC AAGTATGATG TCCAGTGAAC AGCTGAGTCT TGAACTTCAT 
CAAGTGGAAC GGGAGATTGG GAAGAGAACA CGTGAACTCA GCTTGGAAAG CCATTCTTTG 
AGTATGAAAA GCAAGGCAGA TACCATCAAA GTAGAAAATG GGCAACTGGA GGCACCGACA 
AGTGTTCTAT CACATGAACT CCCACTAACA TTCAGCAGTG AGGTGCCAAA TGGATCAGGT 
CTGACACAGG ACATCTCCAT GTTGTCTAGC AAGACAGCAT CTTTAACTCT TTCAGAAGAT 
CCACAGGCTG GAGGAGACAA GAGTGAGCCG CTGAGATCTG GAGTTAATTC CACTGCTGCT 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rc3h1 ( XM_004914024.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914081.1 Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X4, mRNA.

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CloneID	OXa09543
Clone ID Related Accession (Same CDS sequence)	XM_004914024.3 , XM_004914024.4
Accession Version	XM_004914024.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roquin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914024.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGCACAC AGCACAGCCT 
CACCCCAGCC TGGATGAACT TCATAGACGC CGTAAGGAGA TCATGCTTCA GCTAGAAGAA 
AGAAAAGTGA TTTCCCCTCC TCCCTTTGCA CCCTCACCTA CTCTGCCTCA CAGCTTCCAT 
CCAGAAGAGT ACCTTGATGA GGAATTAAAA GTACCTGGGA AATTTAAAGG GAACGATTAC 
AGTCAGTATT CACCCTGGTC CTGTGACACA TTCGGCTCCT ACATTGGAAC CAAAGACAAC 
AAATCCAAAG ATGTTTCATC TGGTGGCAGC GTGGAAGTAG CTAATGTTGA CAGCAAATCC 
CTACGAGACC AACGCATTGA GCTTCAGCGA AGAGCTACAG AGCCTGGTGA GGATGATCTG 
ATTCCCTTTG GAGACAGACC TACCGTTTCT CGCTTTGGGG CCATCTCCCG CACATCGAAG 
GCCCTATACC AACCACCAGG CCCAATGCAG CAGGTCTTGG CAGCCCAAGG AGCTGTAAAA 
TCTAGCATTA CAGATTACAG CTTGTACAGT CACGGTGGCT TGAGTGCTGC ATCATATCCA 
CCCCATCAAA ACCTTTCTTC TCAGGGACAG TTAAGTGAGC GTGAAAGGAT ATCCAAGCCT 
TTACCAACAT CTGAAAGAGA ACAGCTTAGG ATGGAACTGC AGCAAGTTAA CCAGCAGATC 
AGTCAGCAGA CACAAATGCG TGGCATGGAG GTAGCTACTA ACCGCCTGCT GTTGCCAAGA 
GAGGCACCTT CACTTGGAAA CCAGCAGCCA CCTCCACCAC AACCACCAGC CAAGTGGCCA 
AGTATGATGT CCAGTGAACA GCTGAGTCTT GAACTTCATC AAGTGGAACG GGAGATTGGG 
AAGAGAACAC GTGAACTCAG CTTGGAAAGC CATTCTTTGA GTATGAAAAG CAAGGCAGAT 
ACCATCAAAG TAGAAAATGG GCAACTGGAG GCACCGACAA GTGTTCTATC ACATGAACTC 
CCACTAACAT TCAGCAGTGA GGTGCCAAAT GGATCAGGTC TGACACAGGA CATCTCCATG 
TTGTCTAGCA AGACAGCATC TTTAACTCTT TCAGAAGATC CACAGGCTGG AGGAGACAAG 
AGTGAGCCGC TGAGATCTGG AGTTAATTCC ACTGCTGCTC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914081.1
CDS	294..3638
Translation	MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQVPEQQQITLCGGCGAEDTKHYEEARKCVEELALYLKPLSTARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLEDGLVAVKTVVHGLVDYIQNHSKKGTDQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPIGAPDEGPMDLPPRKPSGLPNGIVPGTQLISRSTDSGFESVLKQVKLDQLSSSAPGSPPELLDSVPKASMSALPVNAHPAASRVSSDMPPLPVSKPIQMVPRGSQIYAPPPADTFYQECRGGGPPFDAAPYAQGVYYPPQSSQCVSRFVRPPPAATEPAATYLDHYTPYLQDRVVSTQYGTPTQQYQTIVPTQYQPHYDNRRVYPSNAPPYQREEIVRASPVPLEMPPAAISPYVPESRERYQGIEGYYTHPGQIRPSYHRHTAQPHPSLDELHRRRKEIMLQLEERKVISPPPFAPSPTLPHSFHPEEYLDEELKVPGKFKGNDYSQYSPWSCDTFGSYIGTKDNKSKDVSSGGSVEVANVDSKSLRDQRIELQRRATEPGEDDLIPFGDRPTVSRFGAISRTSKALYQPPGPMQQVLAAQGAVKSSITDYSLYSHGGLSAASYPPHQNLSSQGQLSERERISKPLPTSEREQLRMELQQVNQQISQQTQMRGMEVATNRLLLPREAPSLGNQQPPPPQPPAKWPSMMSSEQLSLELHQVEREIGKRTRELSLESHSLSMKSKADTIKVENGQLEAPTSVLSHELPLTFSSEVPNGSGLTQDISMLSSKTASLTLSEDPQAGGDKSEPLRSGVNSTAAP

Target ORF information:

RefSeq Version	XM_004914024.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger and CCCH-type domains 1 (rc3h1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914024.3

ORF Insert Sequence:

ATGCCTGTAC AAGCACCGCA ATGGACCGAC TTTCTGTCCT GCCCTATTTG CACCCAAACT 
TTCGACGAAA CAATCCGGAA GCCCATCAGT CTAGGCTGTG GACACACAGT TTGCAAGATG 
TGCCTAAACA AGCTTCACCG CAAGGCTTGT CCATTTGATC AGACCACCAT CAACACAGAT 
ATTGAGCTTC TTCCTGTCAA CTCTGCTCTC CTGCAACTGG TAGGAGCTCA GGTGCCTGAA 
CAACAACAGA TCACCCTCTG TGGAGGGTGT GGAGCAGAGG ACACCAAACA TTATGAGGAA 
GCTAGGAAAT GTGTAGAAGA ATTAGCTCTA TATTTAAAAC CCCTTAGCAC TGCAAGAGGT 
GTAGGTCTAA ACAGCACAAC TCAAAGTGTA TTGAGTCGTC CAATGCAAAG GAAACTGGTG 
ACACTTGTGC ATTGTCAGTT GGTTGAGGAG GAAGGACGTA TTAGAGCTAT GAGAGCAGCT 
CGTTCGCTGG GAGAGAGAAC AGTCACTGAG CTTATCTTGC AACACCAAAA TCCTCAGCAG 
CTGTCTTCCA ATCTATGGGC AGCTGTCCGT GCCCGTGGCT GCCAGTTTCT AGGACCAGCC 
ATGCAGGAAG AGGCACTTAA GCTTGTCCTC CTGGCTCTGG AAGATGGATC TGCGCTCTCA 
CGGAAAGTCC TTGTTTTATT TGTGGTGCAG AGACTCGAAC CACGGTTCCC CCAAGCCTCG 
AAAACTAGCA TTGGGCATGT TGTCCAGCTG CTCTACCGAG CCTCTTGCTT TAAGGTAACA 
AAACGTGATG AAGACTCCTC CCTAATGCAG CTGAAGGAGG AATTTCGCAC CTATGAAGCA 
CTGCGTCGAG AGCATGATTC TCAGATTGTT CAAATAGCCA TGGAAGCAGG ATTGCGCATT 
GCTCCTGATC AGTGGTCCTC CCTCCTGTAT GGAGATCAGT CGCATAAATC CCACATGCAG 
TCTATCATTG ACAAGTTGCA GACCCCAGCC TCGTTTGCTC AGAGTGTCCA GGAACTGACC 
ATTGCTTTGC AAAGGACTGG AGACCCCGCA AATCTTAATA GACTTCGACC TCATTTGGAG 
TTGTTAGCGA ACATAGACCC AAGTCCAGAT GCCCCTCCTC CAACATGGGA GCAGTTAGAG 
GATGGTCTGG TGGCAGTGAA AACTGTAGTG CATGGATTAG TGGATTATAT TCAGAATCAT 
AGCAAGAAGG GAACAGACCA ACAGCAGCCT CCACAGCACA GCAAGTACAA AACATACATG 
TGTAGAGATA TGAAACAGAG AGGAGGCTGT CCCCGTGGGG CAAGCTGCAC CTTTGCCCAT 
TCTCAAGAAG AGTTAGAAAA GTTTCGGAAA ATGAACAAAC GTTTGGTTCC AAGACGGCCA 
CTCAGTGCTT CGCTTGGTCA GTTGAATGAG GTTGGACTAC CGATAGGAGC ACCTGATGAA 
GGTCCTATGG ACTTGCCACC CCGAAAGCCA AGTGGGCTTC CCAACGGCAT TGTTCCGGGG 
ACACAGCTCA TCTCAAGGAG TACAGACTCT GGCTTTGAGT CAGTGCTGAA ACAAGTAAAA 
TTGGATCAAT TGAGCAGCAG TGCACCTGGA TCTCCTCCAG AACTGCTGGA TTCTGTCCCC 
AAGGCATCTA TGTCTGCTTT GCCTGTCAAT GCTCATCCTG CTGCTTCGCG TGTATCCAGT 
GATATGCCAC CATTGCCGGT TTCCAAGCCA ATCCAAATGG TGCCACGTGG TTCACAAATA 
TATGCCCCTC CTCCAGCAGA TACGTTTTAC CAAGAATGTC GGGGTGGTGG ACCTCCATTT 
GATGCAGCTC CCTATGCACA AGGTGTTTAT TACCCACCTC AGTCCTCCCA GTGTGTTTCA 
CGCTTTGTCA GACCTCCTCC TGCTGCCACT GAACCAGCCG CCACCTACCT CGACCACTAT 
ACTCCCTACT TACAGGACAG AGTTGTCAGC ACACAGTATG GCACTCCCAC TCAGCAGTAC 
CAGACAATTG TACCCACCCA GTACCAGCCA CATTATGACA ACCGTCGTGT TTATCCCTCT 
AATGCTCCAC CTTACCAAAG GGAGGAAATA GTTCGTGCAA GTCCGGTGCC TCTAGAAATG 
CCTCCTGCTG CTATCTCTCC CTATGTTCCT GAATCCAGAG AGAGATATCA GGGAATTGAA 
GGTTATTATA CACATCCAGG ACAGATAAGG CCATCATATC ATAGGCACAC AGCACAGCCT 
CACCCCAGCC TGGATGAACT TCATAGACGC CGTAAGGAGA TCATGCTTCA GCTAGAAGAA 
AGAAAAGTGA TTTCCCCTCC TCCCTTTGCA CCCTCACCTA CTCTGCCTCA CAGCTTCCAT 
CCAGAAGAGT ACCTTGATGA GGAATTAAAA GTACCTGGGA AATTTAAAGG GAACGATTAC 
AGTCAGTATT CACCCTGGTC CTGTGACACA TTCGGCTCCT ACATTGGAAC CAAAGACAAC 
AAATCCAAAG ATGTTTCATC TGGTGGCAGC GTGGAAGTAG CTAATGTTGA CAGCAAATCC 
CTACGAGACC AACGCATTGA GCTTCAGCGA AGAGCTACAG AGCCTGGTGA GGATGATCTG 
ATTCCCTTTG GAGACAGACC TACCGTTTCT CGCTTTGGGG CCATCTCCCG CACATCGAAG 
GCCCTATACC AACCACCAGG CCCAATGCAG CAGGTCTTGG CAGCCCAAGG AGCTGTAAAA 
TCTAGCATTA CAGATTACAG CTTGTACAGT CACGGTGGCT TGAGTGCTGC ATCATATCCA 
CCCCATCAAA ACCTTTCTTC TCAGGGACAG TTAAGTGAGC GTGAAAGGAT ATCCAAGCCT 
TTACCAACAT CTGAAAGAGA ACAGCTTAGG ATGGAACTGC AGCAAGTTAA CCAGCAGATC 
AGTCAGCAGA CACAAATGCG TGGCATGGAG GTAGCTACTA ACCGCCTGCT GTTGCCAAGA 
GAGGCACCTT CACTTGGAAA CCAGCAGCCA CCTCCACCAC AACCACCAGC CAAGTGGCCA 
AGTATGATGT CCAGTGAACA GCTGAGTCTT GAACTTCATC AAGTGGAACG GGAGATTGGG 
AAGAGAACAC GTGAACTCAG CTTGGAAAGC CATTCTTTGA GTATGAAAAG CAAGGCAGAT 
ACCATCAAAG TAGAAAATGG GCAACTGGAG GCACCGACAA GTGTTCTATC ACATGAACTC 
CCACTAACAT TCAGCAGTGA GGTGCCAAAT GGATCAGGTC TGACACAGGA CATCTCCATG 
TTGTCTAGCA AGACAGCATC TTTAACTCTT TCAGAAGATC CACAGGCTGG AGGAGACAAG 
AGTGAGCCGC TGAGATCTGG AGTTAATTCC ACTGCTGCTC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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rc3h1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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