sema3b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema3b
Entrez Gene ID	100151737
Full Name	semaphorin 3B
Synonyms	luca-1,sema,sema5,semaa,semav
General protein information	Preferred Names semaphorin 3B Names semaphorin-3B sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012964329.2	XP_012819783.1	semaphorin-3B isoform X1
XM_031899870.1	XP_031755730.1	semaphorin-3B isoform X1
XM_012964329.3	XP_012819783.1	semaphorin-3B isoform X1
NM_001127436.1	NP_001120908.1	semaphorin-3B precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SEMA3B	NP_001192676.1
Danio rerio (zebrafish)	si:ch211-106g4.2	NP_001121818.1
Mus musculus (house mouse)	Sema3b	NP_033179.2
Xenopus tropicalis (tropical clawed frog)	sema3b	NP_001120908.1
Gallus gallus (chicken)	SEMA3B	XP_423897.4
Homo sapiens (human)	SEMA3B	NP_004627.1
Pan troglodytes (chimpanzee)	SEMA3B	XP_001149891.3
Canis lupus familiaris (dog)	SEMA3B	XP_005633247.1
Rattus norvegicus (Norway rat)	Sema3b	NP_001073411.1
	SEMA3B	XP_001102773.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sema3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa24403	NM_001127436.1 Latest version!	Xenopus tropicalis semaphorin 3B (sema3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa32103	XM_031899870.1 Latest version!	Xenopus tropicalis semaphorin 3B (sema3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa32103	XM_012964329.3 Latest version!	Xenopus tropicalis semaphorin 3B (sema3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa32103	XM_012964329.2	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (sema3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema3b ( NM_001127436.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001120908.1 Xenopus tropicalis semaphorin 3B (sema3b), mRNA.

Price & Availability↑

CloneID	OXa24403
Clone ID Related Accession (Same CDS sequence)	NM_001127436.1
Accession Version	NM_001127436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	semaphorin-3B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166183.1. ##Evidence-Data-START## Transcript exon combination :: BC166183.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028293, SAMD00028294 [ECO:0000348] ##Evidence-Data-END##

ATGAAACTCC AGCGTAGCCT CCTTTCTCTG TGCCTTCTGC TGTATTTTGG CCTCATTGGG 
AGCAACAAGG TCACGCCCCG GCTGAAATTG TCCTATCAGG ATCTGATGTC TCTGCATGGA 
CTCAGGACAT TTACTCTAGA GCGCTCTTGC TGTTATGACG CACTCTTCCT AGATGAGGAG 
CGTGGCCGTC TCTTTGTGGG GGGGAAAAAC TACCTGATGT CTCTCAGCCT GGATAATATC 
AGCAAACAAG AACTGGCAAT TCACTGGCCG GCACCGGTGG AGTGGAGAGA AGAGTGTAAC 
TGGGCTGGAA AGGATATCAA TGTAGACTGT ATGAACTACG TGAAGATCCT GCAGCCGTAC 
AACCGGACTC ATTTGTTCAC CTGTGGGACT GGAGCTTTCC ATCCAACTTG TGGATTTGTC 
CATGTTGGGC AGCGAACAGA TGACACAGTA TTTAAAATGG ATCTGAGAAG GATGGAGGAC 
GGGAAGGGGC GCTGTCCATA TGACCCCAAA CATATAGCAG CTGCCGTACT AGTAGGAGAT 
GAACTATATT CTGGAGTTGC AACGGACCTT ATGGGCCGGG ATTTCACCAT ATTTCGCAGC 
CTGGGTAGGC GCCCTCCCTT GCGCACAGAG CAGCATGATT CCCGTTGGCT AAACGAACCC 
AAGTTTGTGA AAGTTTTCTG GATTCCTGAG AGTGAAAATC CAGATGACGA TAAAATCTAC 
TTCTTCTTCA AGGAGACACC AGTGGAAGGA GCCGGTGGAG TGAAGCAGTC GTACGCTCGC 
ATTGGGCAAA TCTGCAGGAA TGACTTGGGA GGTCAGAGGA GCCTGGTGAA CAAATGGACA 
ACTTTCCTGA AGGCCCGACT TGTGTGTAGC GTACCTGGGA ATGAAATAGG AGGGGACACA 
CATTTTGATG AGCTCCGGGA TGTCTTCCTT TTACAAACTC GGGACAGAAG AAATCCTCTC 
ATTTATGCTT TGTTTTCCAC ATCAAGCTCT GTCTTCCGTG GCTCTGCCAT ATGTGTCTAC 
ACCATGAATG ATGTGCGTAG AGCTTTCCTG GGACCCTTTG CTCATAAGGA AGGACCTAAC 
TATCAGTGGG TGTCATATCA GGGCAAGGTG CCATATCCTC GTCCCGGGAT GTGTCCCAGT 
AAGACATTTG GAAGCTTTGA GTCCACAAAG AGTTTCCCAG ATGATGTCCT TCAGTTTGCT 
CGGAATCACC CACTCATGTT CAACCCTGTG GTACCTGGGT CGGGCAGGCC TGTCTTCTTG 
CGCAGTCATG GAGACCTCAG TTTCACGCAG CTGGCTGTGG ACAGAGTGGC TGCTGCTGAT 
GGAGAGTATG ATGTCATGTT TATTGGGACA GATGTTGGTT CAGTGCTGAA AGTGATATCA 
GTGCCCAAGC AAAGCTGGCA TCACGTGGAA CAAGTTGTAC TGGAGGAGCT ACAAGTCTTC 
AAGAATGCAT CTCCTATAAC TACCATGCAG ATCTCCTCCA AGCGGCAACA GATATACGTG 
GGATCTGCAA GTGGCATCTC CCAGCTGCCC CTGCACCGCT GTGGCATCTA TGGCAAGGCA 
TGTGCAGAGT GTTGCTTGGC CAGGGATCCC TATTGTGCTT GGGATGGTGC CAGCTGTACA 
CGTTTTCTGC CCAACACTAA GAGGAGATCT CGTCGCCAAG ATGTGAGGAA TGGAGACCCC 
AACACCCTTT GTTCAGGAGA GCAGCAGAGA TTTAGCGTGC AGGAGAAACG TCTGTTGGGG 
GTTGAAGGGA GCAGCATATT CCTTGAGTGT CTTCCAAAAT CTCTACGTGC TCAAGTCACT 
TGGACATATC AGAAGGCACC AGAATCTTCA AGAACAGAGG TTCAGTTTGA TGAGCGTGTG 
CTTCAGACAG ACCGTGGTAT TGTTGTACGC AATGTATTAC GCCGTGATGC AGGCACCTTC 
ATATGTTACT CTTCAGAACA TGGATTTACA CAAACCCTTC TCCGCCTTCA CTTAGAGGTT 
ATTCCTTCCT CCAGGACTGA AGAACACTTA GGGCTCCAGC GTGTTCCGCA CAATCGATGG 
TACCAGGATT TCATGCGTCT AGTACAGCAG CCAGGCTCTG TGGAGCAAAT GTGCGAGGAG 
ATATGGACCC GCAAGGGCCG AACCTCACAT CCACCTGGAC CCCAGGGGGC CCCTCATGTT 
CCAGGTTCCC AAGGACGAAG TCTCCCAGCT GTGCCACCCC TCAATATTAA AACCAAAGCT 
CAAAAGTGGA AGCACTTAGA GGATAAGAGG AAGGTGCGGA GCCGCCGGAC GCATGAGCAG 
CTTAGCCGGG CTGAACGTGG TCCCAGAAGT GCCCCAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120908.1
CDS	136..2457
Translation	MKLQRSLLSLCLLLYFGLIGSNKVTPRLKLSYQDLMSLHGLRTFTLERSCCYDALFLDEERGRLFVGGKNYLMSLSLDNISKQELAIHWPAPVEWREECNWAGKDINVDCMNYVKILQPYNRTHLFTCGTGAFHPTCGFVHVGQRTDDTVFKMDLRRMEDGKGRCPYDPKHIAAAVLVGDELYSGVATDLMGRDFTIFRSLGRRPPLRTEQHDSRWLNEPKFVKVFWIPESENPDDDKIYFFFKETPVEGAGGVKQSYARIGQICRNDLGGQRSLVNKWTTFLKARLVCSVPGNEIGGDTHFDELRDVFLLQTRDRRNPLIYALFSTSSSVFRGSAICVYTMNDVRRAFLGPFAHKEGPNYQWVSYQGKVPYPRPGMCPSKTFGSFESTKSFPDDVLQFARNHPLMFNPVVPGSGRPVFLRSHGDLSFTQLAVDRVAAADGEYDVMFIGTDVGSVLKVISVPKQSWHHVEQVVLEELQVFKNASPITTMQISSKRQQIYVGSASGISQLPLHRCGIYGKACAECCLARDPYCAWDGASCTRFLPNTKRRSRRQDVRNGDPNTLCSGEQQRFSVQEKRLLGVEGSSIFLECLPKSLRAQVTWTYQKAPESSRTEVQFDERVLQTDRGIVVRNVLRRDAGTFICYSSEHGFTQTLLRLHLEVIPSSRTEEHLGLQRVPHNRWYQDFMRLVQQPGSVEQMCEEIWTRKGRTSHPPGPQGAPHVPGSQGRSLPAVPPLNIKTKAQKWKHLEDKRKVRSRRTHEQLSRAERGPRSAPS

Target ORF information:

RefSeq Version	NM_001127436.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis semaphorin 3B (sema3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127436.1

ORF Insert Sequence:

ATGAAACTCC AGCGTAGCCT CCTTTCTCTG TGCCTTCTGC TGTATTTTGG CCTCATTGGG 
AGCAACAAGG TCACGCCCCG GCTGAAATTG TCCTATCAGG ATCTGATGTC TCTGCATGGA 
CTCAGGACAT TTACTCTAGA GCGCTCTTGC TGTTATGACG CACTCTTCCT AGATGAGGAG 
CGTGGCCGTC TCTTTGTGGG GGGGAAAAAC TACCTGATGT CTCTCAGCCT GGATAATATC 
AGCAAACAAG AACTGGCAAT TCACTGGCCG GCACCGGTGG AGTGGAGAGA AGAGTGTAAC 
TGGGCTGGAA AGGATATCAA TGTAGACTGT ATGAACTACG TGAAGATCCT GCAGCCGTAC 
AACCGGACTC ATTTGTTCAC CTGTGGGACT GGAGCTTTCC ATCCAACTTG TGGATTTGTC 
CATGTTGGGC AGCGAACAGA TGACACAGTA TTTAAAATGG ATCTGAGAAG GATGGAGGAC 
GGGAAGGGGC GCTGTCCATA TGACCCCAAA CATATAGCAG CTGCCGTACT AGTAGGAGAT 
GAACTATATT CTGGAGTTGC AACGGACCTT ATGGGCCGGG ATTTCACCAT ATTTCGCAGC 
CTGGGTAGGC GCCCTCCCTT GCGCACAGAG CAGCATGATT CCCGTTGGCT AAACGAACCC 
AAGTTTGTGA AAGTTTTCTG GATTCCTGAG AGTGAAAATC CAGATGACGA TAAAATCTAC 
TTCTTCTTCA AGGAGACACC AGTGGAAGGA GCCGGTGGAG TGAAGCAGTC GTACGCTCGC 
ATTGGGCAAA TCTGCAGGAA TGACTTGGGA GGTCAGAGGA GCCTGGTGAA CAAATGGACA 
ACTTTCCTGA AGGCCCGACT TGTGTGTAGC GTACCTGGGA ATGAAATAGG AGGGGACACA 
CATTTTGATG AGCTCCGGGA TGTCTTCCTT TTACAAACTC GGGACAGAAG AAATCCTCTC 
ATTTATGCTT TGTTTTCCAC ATCAAGCTCT GTCTTCCGTG GCTCTGCCAT ATGTGTCTAC 
ACCATGAATG ATGTGCGTAG AGCTTTCCTG GGACCCTTTG CTCATAAGGA AGGACCTAAC 
TATCAGTGGG TGTCATATCA GGGCAAGGTG CCATATCCTC GTCCCGGGAT GTGTCCCAGT 
AAGACATTTG GAAGCTTTGA GTCCACAAAG AGTTTCCCAG ATGATGTCCT TCAGTTTGCT 
CGGAATCACC CACTCATGTT CAACCCTGTG GTACCTGGGT CGGGCAGGCC TGTCTTCTTG 
CGCAGTCATG GAGACCTCAG TTTCACGCAG CTGGCTGTGG ACAGAGTGGC TGCTGCTGAT 
GGAGAGTATG ATGTCATGTT TATTGGGACA GATGTTGGTT CAGTGCTGAA AGTGATATCA 
GTGCCCAAGC AAAGCTGGCA TCACGTGGAA CAAGTTGTAC TGGAGGAGCT ACAAGTCTTC 
AAGAATGCAT CTCCTATAAC TACCATGCAG ATCTCCTCCA AGCGGCAACA GATATACGTG 
GGATCTGCAA GTGGCATCTC CCAGCTGCCC CTGCACCGCT GTGGCATCTA TGGCAAGGCA 
TGTGCAGAGT GTTGCTTGGC CAGGGATCCC TATTGTGCTT GGGATGGTGC CAGCTGTACA 
CGTTTTCTGC CCAACACTAA GAGGAGATCT CGTCGCCAAG ATGTGAGGAA TGGAGACCCC 
AACACCCTTT GTTCAGGAGA GCAGCAGAGA TTTAGCGTGC AGGAGAAACG TCTGTTGGGG 
GTTGAAGGGA GCAGCATATT CCTTGAGTGT CTTCCAAAAT CTCTACGTGC TCAAGTCACT 
TGGACATATC AGAAGGCACC AGAATCTTCA AGAACAGAGG TTCAGTTTGA TGAGCGTGTG 
CTTCAGACAG ACCGTGGTAT TGTTGTACGC AATGTATTAC GCCGTGATGC AGGCACCTTC 
ATATGTTACT CTTCAGAACA TGGATTTACA CAAACCCTTC TCCGCCTTCA CTTAGAGGTT 
ATTCCTTCCT CCAGGACTGA AGAACACTTA GGGCTCCAGC GTGTTCCGCA CAATCGATGG 
TACCAGGATT TCATGCGTCT AGTACAGCAG CCAGGCTCTG TGGAGCAAAT GTGCGAGGAG 
ATATGGACCC GCAAGGGCCG AACCTCACAT CCACCTGGAC CCCAGGGGGC CCCTCATGTT 
CCAGGTTCCC AAGGACGAAG TCTCCCAGCT GTGCCACCCC TCAATATTAA AACCAAAGCT 
CAAAAGTGGA AGCACTTAGA GGATAAGAGG AAGGTGCGGA GCCGCCGGAC GCATGAGCAG 
CTTAGCCGGG CTGAACGTGG TCCCAGAAGT GCCCCAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3b ( XM_012964329.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819783.1 Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (sema3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa32103
Clone ID Related Accession (Same CDS sequence)	XM_012964329.2 , XM_031899870.1 , XM_012964329.3
Accession Version	XM_012964329.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	semaphorin-3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTCC AGCGTAGCCT CCTTTCTCTG TGCCTTCTGC TGTATTTTGG CCTCATTGGG 
AGCAACAAGG TCACGCCCCG GCTGAAATTG TCCTATCAGG ATCTGATGTC TCTGCATGGA 
CTCAGGACAT TTACACTAGA GCGCTCTTGC TGTTATGACG CACTCTTCCT AGATGAGGAG 
CGTGGCCGTC TCTTTGTGGG GGGGAAAAAC TACCTGATGT CTCTCAGCCT GGATAATATC 
AGCAAACAAG AACTGGCAAT TCACTGGCCG GCACCGGTGG AGTGGAGAGA AGAGTGTAAC 
TGGGCTGGAA AGGATATCAA TATAGACTGT ATGAACTACG TGAAGATCCT GCAGCCGTAC 
AACCGGACTC ATTTGTTCAC CTGTGGGACT GGAGCTTTCC ATCCAACTTG TGGATTTGTC 
CATGTTGGGC AGCGAACAGA TGACACAGTA TTTAAAATGG ATCTGAGAAG GATGGAGGAC 
GGGAAGGGGC GCTGTCCATA TGACCCCAAA CATATAGCAG CTGCCGTACT AGTAGGAGAT 
GAACTATATT CTGGAGTTGC AACGGACCTT ATGGGCCGGG ATTTCACCAT ATTTCGCAGC 
CTGGGTAGGC GCCCTCCCTT GCGCACAGAG CAGCATGATT CCCGTTGGCT AAACGAACCC 
AAGTTTGTGA AAGTTTTCTG GATTCCTGAG AGTGAAAATC CAGATGACGA TAAAATCTAC 
TTCTTCTTCA AGGAGACACC AGTGGAAGGA GCCGGTGGAG TGAAGCAGTC GTACGCTCGC 
ATTGGGCAAA TCTGCAGGAA TGACTTGGGA GGTCAGAGGA GCCTGGTGAA CAAATGGACA 
ACTTTCCTGA AGGCCCGACT TGTGTGTAGC GTACCTGGGA ATGAAATAGG AGGGGACACA 
CATTTTGATG AGCTCCGGGA TGTCTTCCTT TTACAAACTC GGGACAGAAG AAATCCTCTC 
ATTTATGCTT TGTTTTCCAC ATCAAGCTCT GTCTTCCGTG GCTCTGCCAT ATGTGTCTAC 
ACCATGAATG ATGTGCGTAG AGCTTTCCTG GGACCCTTTG CTCATAAGGA AGGACCTAAC 
TATCAGTGGG TGTCATATCA GGGCAAGGTG CCATATCCTC GTCCCGGGAT GTGTCCCAGT 
AAGACATTTG GAAGCTTTGA GTCCACAAAG AGTTTCCCAG ATGATGTCCT TCAGTTTGCT 
CGGAATCACC CACTCATGTT CAACCCTGTG GTACCTGGGT CGGGCAGGCC TGTCTTCTTG 
CGCAGTCATG GAGACCTCAG TTTCACGCAG CTGGCTGTGG ACAGAGTGGC TGCTGCTGAT 
GGAGAGTATG ATGTCATGTT TATTGGGACA GATGTTGGTT CAGTGCTGAA AGTGATATCA 
GTGCCCAAGC AAAGCTGGCA TCACGTGGAA CAAGTTGTAC TGGAGGAGCT ACAAGTCTTC 
AAGAATGCAT CTCCTATAAC TACCATGCAG ATCTCCTCCA AGCGGCAACA GATATACGTG 
GGATCTGCAA GTGGCATCTC CCAGCTGCCC CTGCACCGCT GTGGCATCTA TGGCAAGGCA 
TGTGCAGAGT GTTGCTTGGC CAGGGATCCC TATTGTGCTT GGGATGGTGC CAGCTGTACA 
CGTTTTCTGC CCAACACTAA GAGGAGATCT CGTCGCCAAG ATGTGAGGAA TGGAGACCCC 
AACACCCTTT GTTCAGGAGA GCAGCAGAGA TTTAGCGTGC AGGAGAAACG TCTGTTGGGG 
GTTGAAGGGA GCAGCATATT CCTTGAGTGT CTTCCAAAAT CTCTACGTGC TCAAGTCACT 
TGGACATATC AGAAGGCACC AGAATCTTCA AGAACAGAGG TTCAGTTTGA TGAGCGTGTG 
CTTCAGACAG ACCGTGGTAT TGTTGTACGC AATGTATTAC GCCGTGATGC AGGCACCTTC 
ATATGTTACT CTTCAGAACA TGGATTTACA CAAACCCTTC TCCGCCTTCA CTTAGAGGTT 
ATTCCTTCCT CCAGGACTGA AGAACACTTA GGGCTCCAGC GTGTTCCGCA CAATCGATGG 
TACCAGGATT TCATGCGTCT AGTACAGCAG CCAGGCTCTG TGGAGCAAAT GTGCGAGGAG 
ATATGGACCC GCAAGGGCCG AACCTCACAT CCACCTGGAC CCCAGGGGGC CCCTCATGTT 
CCAGGTTCCC AAGGACGAAG TCTCCCAGCT GTGCCACCCC TCAATATTAA AACCAAAGCT 
CAAAAGTGGA AGCACTTAGA GGATAAGAGG AAGGTGCGGA GCCGCCGGAC GCATGAGCAG 
CTTAGCCGGG CTGAACGTGG TCCCAGAAGT GCCCCAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819783.1
CDS	142..2463
Translation	MKLQRSLLSLCLLLYFGLIGSNKVTPRLKLSYQDLMSLHGLRTFTLERSCCYDALFLDEERGRLFVGGKNYLMSLSLDNISKQELAIHWPAPVEWREECNWAGKDINIDCMNYVKILQPYNRTHLFTCGTGAFHPTCGFVHVGQRTDDTVFKMDLRRMEDGKGRCPYDPKHIAAAVLVGDELYSGVATDLMGRDFTIFRSLGRRPPLRTEQHDSRWLNEPKFVKVFWIPESENPDDDKIYFFFKETPVEGAGGVKQSYARIGQICRNDLGGQRSLVNKWTTFLKARLVCSVPGNEIGGDTHFDELRDVFLLQTRDRRNPLIYALFSTSSSVFRGSAICVYTMNDVRRAFLGPFAHKEGPNYQWVSYQGKVPYPRPGMCPSKTFGSFESTKSFPDDVLQFARNHPLMFNPVVPGSGRPVFLRSHGDLSFTQLAVDRVAAADGEYDVMFIGTDVGSVLKVISVPKQSWHHVEQVVLEELQVFKNASPITTMQISSKRQQIYVGSASGISQLPLHRCGIYGKACAECCLARDPYCAWDGASCTRFLPNTKRRSRRQDVRNGDPNTLCSGEQQRFSVQEKRLLGVEGSSIFLECLPKSLRAQVTWTYQKAPESSRTEVQFDERVLQTDRGIVVRNVLRRDAGTFICYSSEHGFTQTLLRLHLEVIPSSRTEEHLGLQRVPHNRWYQDFMRLVQQPGSVEQMCEEIWTRKGRTSHPPGPQGAPHVPGSQGRSLPAVPPLNIKTKAQKWKHLEDKRKVRSRRTHEQLSRAERGPRSAPS

Target ORF information:

RefSeq Version	XM_012964329.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (sema3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964329.2

ORF Insert Sequence:

ATGAAACTCC AGCGTAGCCT CCTTTCTCTG TGCCTTCTGC TGTATTTTGG CCTCATTGGG 
AGCAACAAGG TCACGCCCCG GCTGAAATTG TCCTATCAGG ATCTGATGTC TCTGCATGGA 
CTCAGGACAT TTACACTAGA GCGCTCTTGC TGTTATGACG CACTCTTCCT AGATGAGGAG 
CGTGGCCGTC TCTTTGTGGG GGGGAAAAAC TACCTGATGT CTCTCAGCCT GGATAATATC 
AGCAAACAAG AACTGGCAAT TCACTGGCCG GCACCGGTGG AGTGGAGAGA AGAGTGTAAC 
TGGGCTGGAA AGGATATCAA TATAGACTGT ATGAACTACG TGAAGATCCT GCAGCCGTAC 
AACCGGACTC ATTTGTTCAC CTGTGGGACT GGAGCTTTCC ATCCAACTTG TGGATTTGTC 
CATGTTGGGC AGCGAACAGA TGACACAGTA TTTAAAATGG ATCTGAGAAG GATGGAGGAC 
GGGAAGGGGC GCTGTCCATA TGACCCCAAA CATATAGCAG CTGCCGTACT AGTAGGAGAT 
GAACTATATT CTGGAGTTGC AACGGACCTT ATGGGCCGGG ATTTCACCAT ATTTCGCAGC 
CTGGGTAGGC GCCCTCCCTT GCGCACAGAG CAGCATGATT CCCGTTGGCT AAACGAACCC 
AAGTTTGTGA AAGTTTTCTG GATTCCTGAG AGTGAAAATC CAGATGACGA TAAAATCTAC 
TTCTTCTTCA AGGAGACACC AGTGGAAGGA GCCGGTGGAG TGAAGCAGTC GTACGCTCGC 
ATTGGGCAAA TCTGCAGGAA TGACTTGGGA GGTCAGAGGA GCCTGGTGAA CAAATGGACA 
ACTTTCCTGA AGGCCCGACT TGTGTGTAGC GTACCTGGGA ATGAAATAGG AGGGGACACA 
CATTTTGATG AGCTCCGGGA TGTCTTCCTT TTACAAACTC GGGACAGAAG AAATCCTCTC 
ATTTATGCTT TGTTTTCCAC ATCAAGCTCT GTCTTCCGTG GCTCTGCCAT ATGTGTCTAC 
ACCATGAATG ATGTGCGTAG AGCTTTCCTG GGACCCTTTG CTCATAAGGA AGGACCTAAC 
TATCAGTGGG TGTCATATCA GGGCAAGGTG CCATATCCTC GTCCCGGGAT GTGTCCCAGT 
AAGACATTTG GAAGCTTTGA GTCCACAAAG AGTTTCCCAG ATGATGTCCT TCAGTTTGCT 
CGGAATCACC CACTCATGTT CAACCCTGTG GTACCTGGGT CGGGCAGGCC TGTCTTCTTG 
CGCAGTCATG GAGACCTCAG TTTCACGCAG CTGGCTGTGG ACAGAGTGGC TGCTGCTGAT 
GGAGAGTATG ATGTCATGTT TATTGGGACA GATGTTGGTT CAGTGCTGAA AGTGATATCA 
GTGCCCAAGC AAAGCTGGCA TCACGTGGAA CAAGTTGTAC TGGAGGAGCT ACAAGTCTTC 
AAGAATGCAT CTCCTATAAC TACCATGCAG ATCTCCTCCA AGCGGCAACA GATATACGTG 
GGATCTGCAA GTGGCATCTC CCAGCTGCCC CTGCACCGCT GTGGCATCTA TGGCAAGGCA 
TGTGCAGAGT GTTGCTTGGC CAGGGATCCC TATTGTGCTT GGGATGGTGC CAGCTGTACA 
CGTTTTCTGC CCAACACTAA GAGGAGATCT CGTCGCCAAG ATGTGAGGAA TGGAGACCCC 
AACACCCTTT GTTCAGGAGA GCAGCAGAGA TTTAGCGTGC AGGAGAAACG TCTGTTGGGG 
GTTGAAGGGA GCAGCATATT CCTTGAGTGT CTTCCAAAAT CTCTACGTGC TCAAGTCACT 
TGGACATATC AGAAGGCACC AGAATCTTCA AGAACAGAGG TTCAGTTTGA TGAGCGTGTG 
CTTCAGACAG ACCGTGGTAT TGTTGTACGC AATGTATTAC GCCGTGATGC AGGCACCTTC 
ATATGTTACT CTTCAGAACA TGGATTTACA CAAACCCTTC TCCGCCTTCA CTTAGAGGTT 
ATTCCTTCCT CCAGGACTGA AGAACACTTA GGGCTCCAGC GTGTTCCGCA CAATCGATGG 
TACCAGGATT TCATGCGTCT AGTACAGCAG CCAGGCTCTG TGGAGCAAAT GTGCGAGGAG 
ATATGGACCC GCAAGGGCCG AACCTCACAT CCACCTGGAC CCCAGGGGGC CCCTCATGTT 
CCAGGTTCCC AAGGACGAAG TCTCCCAGCT GTGCCACCCC TCAATATTAA AACCAAAGCT 
CAAAAGTGGA AGCACTTAGA GGATAAGAGG AAGGTGCGGA GCCGCCGGAC GCATGAGCAG 
CTTAGCCGGG CTGAACGTGG TCCCAGAAGT GCCCCAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3b ( XM_031899870.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755730.1 Xenopus tropicalis semaphorin 3B (sema3b), transcript variant X1, mRNA.

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CloneID	OXa32103
Clone ID Related Accession (Same CDS sequence)	XM_012964329.2 , XM_031899870.1 , XM_012964329.3
Accession Version	XM_031899870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	semaphorin-3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTCC AGCGTAGCCT CCTTTCTCTG TGCCTTCTGC TGTATTTTGG CCTCATTGGG 
AGCAACAAGG TCACGCCCCG GCTGAAATTG TCCTATCAGG ATCTGATGTC TCTGCATGGA 
CTCAGGACAT TTACACTAGA GCGCTCTTGC TGTTATGACG CACTCTTCCT AGATGAGGAG 
CGTGGCCGTC TCTTTGTGGG GGGGAAAAAC TACCTGATGT CTCTCAGCCT GGATAATATC 
AGCAAACAAG AACTGGCAAT TCACTGGCCG GCACCGGTGG AGTGGAGAGA AGAGTGTAAC 
TGGGCTGGAA AGGATATCAA TATAGACTGT ATGAACTACG TGAAGATCCT GCAGCCGTAC 
AACCGGACTC ATTTGTTCAC CTGTGGGACT GGAGCTTTCC ATCCAACTTG TGGATTTGTC 
CATGTTGGGC AGCGAACAGA TGACACAGTA TTTAAAATGG ATCTGAGAAG GATGGAGGAC 
GGGAAGGGGC GCTGTCCATA TGACCCCAAA CATATAGCAG CTGCCGTACT AGTAGGAGAT 
GAACTATATT CTGGAGTTGC AACGGACCTT ATGGGCCGGG ATTTCACCAT ATTTCGCAGC 
CTGGGTAGGC GCCCTCCCTT GCGCACAGAG CAGCATGATT CCCGTTGGCT AAACGAACCC 
AAGTTTGTGA AAGTTTTCTG GATTCCTGAG AGTGAAAATC CAGATGACGA TAAAATCTAC 
TTCTTCTTCA AGGAGACACC AGTGGAAGGA GCCGGTGGAG TGAAGCAGTC GTACGCTCGC 
ATTGGGCAAA TCTGCAGGAA TGACTTGGGA GGTCAGAGGA GCCTGGTGAA CAAATGGACA 
ACTTTCCTGA AGGCCCGACT TGTGTGTAGC GTACCTGGGA ATGAAATAGG AGGGGACACA 
CATTTTGATG AGCTCCGGGA TGTCTTCCTT TTACAAACTC GGGACAGAAG AAATCCTCTC 
ATTTATGCTT TGTTTTCCAC ATCAAGCTCT GTCTTCCGTG GCTCTGCCAT ATGTGTCTAC 
ACCATGAATG ATGTGCGTAG AGCTTTCCTG GGACCCTTTG CTCATAAGGA AGGACCTAAC 
TATCAGTGGG TGTCATATCA GGGCAAGGTG CCATATCCTC GTCCCGGGAT GTGTCCCAGT 
AAGACATTTG GAAGCTTTGA GTCCACAAAG AGTTTCCCAG ATGATGTCCT TCAGTTTGCT 
CGGAATCACC CACTCATGTT CAACCCTGTG GTACCTGGGT CGGGCAGGCC TGTCTTCTTG 
CGCAGTCATG GAGACCTCAG TTTCACGCAG CTGGCTGTGG ACAGAGTGGC TGCTGCTGAT 
GGAGAGTATG ATGTCATGTT TATTGGGACA GATGTTGGTT CAGTGCTGAA AGTGATATCA 
GTGCCCAAGC AAAGCTGGCA TCACGTGGAA CAAGTTGTAC TGGAGGAGCT ACAAGTCTTC 
AAGAATGCAT CTCCTATAAC TACCATGCAG ATCTCCTCCA AGCGGCAACA GATATACGTG 
GGATCTGCAA GTGGCATCTC CCAGCTGCCC CTGCACCGCT GTGGCATCTA TGGCAAGGCA 
TGTGCAGAGT GTTGCTTGGC CAGGGATCCC TATTGTGCTT GGGATGGTGC CAGCTGTACA 
CGTTTTCTGC CCAACACTAA GAGGAGATCT CGTCGCCAAG ATGTGAGGAA TGGAGACCCC 
AACACCCTTT GTTCAGGAGA GCAGCAGAGA TTTAGCGTGC AGGAGAAACG TCTGTTGGGG 
GTTGAAGGGA GCAGCATATT CCTTGAGTGT CTTCCAAAAT CTCTACGTGC TCAAGTCACT 
TGGACATATC AGAAGGCACC AGAATCTTCA AGAACAGAGG TTCAGTTTGA TGAGCGTGTG 
CTTCAGACAG ACCGTGGTAT TGTTGTACGC AATGTATTAC GCCGTGATGC AGGCACCTTC 
ATATGTTACT CTTCAGAACA TGGATTTACA CAAACCCTTC TCCGCCTTCA CTTAGAGGTT 
ATTCCTTCCT CCAGGACTGA AGAACACTTA GGGCTCCAGC GTGTTCCGCA CAATCGATGG 
TACCAGGATT TCATGCGTCT AGTACAGCAG CCAGGCTCTG TGGAGCAAAT GTGCGAGGAG 
ATATGGACCC GCAAGGGCCG AACCTCACAT CCACCTGGAC CCCAGGGGGC CCCTCATGTT 
CCAGGTTCCC AAGGACGAAG TCTCCCAGCT GTGCCACCCC TCAATATTAA AACCAAAGCT 
CAAAAGTGGA AGCACTTAGA GGATAAGAGG AAGGTGCGGA GCCGCCGGAC GCATGAGCAG 
CTTAGCCGGG CTGAACGTGG TCCCAGAAGT GCCCCAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755730.1
CDS	107..2428
Translation	MKLQRSLLSLCLLLYFGLIGSNKVTPRLKLSYQDLMSLHGLRTFTLERSCCYDALFLDEERGRLFVGGKNYLMSLSLDNISKQELAIHWPAPVEWREECNWAGKDINIDCMNYVKILQPYNRTHLFTCGTGAFHPTCGFVHVGQRTDDTVFKMDLRRMEDGKGRCPYDPKHIAAAVLVGDELYSGVATDLMGRDFTIFRSLGRRPPLRTEQHDSRWLNEPKFVKVFWIPESENPDDDKIYFFFKETPVEGAGGVKQSYARIGQICRNDLGGQRSLVNKWTTFLKARLVCSVPGNEIGGDTHFDELRDVFLLQTRDRRNPLIYALFSTSSSVFRGSAICVYTMNDVRRAFLGPFAHKEGPNYQWVSYQGKVPYPRPGMCPSKTFGSFESTKSFPDDVLQFARNHPLMFNPVVPGSGRPVFLRSHGDLSFTQLAVDRVAAADGEYDVMFIGTDVGSVLKVISVPKQSWHHVEQVVLEELQVFKNASPITTMQISSKRQQIYVGSASGISQLPLHRCGIYGKACAECCLARDPYCAWDGASCTRFLPNTKRRSRRQDVRNGDPNTLCSGEQQRFSVQEKRLLGVEGSSIFLECLPKSLRAQVTWTYQKAPESSRTEVQFDERVLQTDRGIVVRNVLRRDAGTFICYSSEHGFTQTLLRLHLEVIPSSRTEEHLGLQRVPHNRWYQDFMRLVQQPGSVEQMCEEIWTRKGRTSHPPGPQGAPHVPGSQGRSLPAVPPLNIKTKAQKWKHLEDKRKVRSRRTHEQLSRAERGPRSAPS

Target ORF information:

RefSeq Version	XM_031899870.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis semaphorin 3B (sema3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899870.1

ORF Insert Sequence:

ATGAAACTCC AGCGTAGCCT CCTTTCTCTG TGCCTTCTGC TGTATTTTGG CCTCATTGGG 
AGCAACAAGG TCACGCCCCG GCTGAAATTG TCCTATCAGG ATCTGATGTC TCTGCATGGA 
CTCAGGACAT TTACACTAGA GCGCTCTTGC TGTTATGACG CACTCTTCCT AGATGAGGAG 
CGTGGCCGTC TCTTTGTGGG GGGGAAAAAC TACCTGATGT CTCTCAGCCT GGATAATATC 
AGCAAACAAG AACTGGCAAT TCACTGGCCG GCACCGGTGG AGTGGAGAGA AGAGTGTAAC 
TGGGCTGGAA AGGATATCAA TATAGACTGT ATGAACTACG TGAAGATCCT GCAGCCGTAC 
AACCGGACTC ATTTGTTCAC CTGTGGGACT GGAGCTTTCC ATCCAACTTG TGGATTTGTC 
CATGTTGGGC AGCGAACAGA TGACACAGTA TTTAAAATGG ATCTGAGAAG GATGGAGGAC 
GGGAAGGGGC GCTGTCCATA TGACCCCAAA CATATAGCAG CTGCCGTACT AGTAGGAGAT 
GAACTATATT CTGGAGTTGC AACGGACCTT ATGGGCCGGG ATTTCACCAT ATTTCGCAGC 
CTGGGTAGGC GCCCTCCCTT GCGCACAGAG CAGCATGATT CCCGTTGGCT AAACGAACCC 
AAGTTTGTGA AAGTTTTCTG GATTCCTGAG AGTGAAAATC CAGATGACGA TAAAATCTAC 
TTCTTCTTCA AGGAGACACC AGTGGAAGGA GCCGGTGGAG TGAAGCAGTC GTACGCTCGC 
ATTGGGCAAA TCTGCAGGAA TGACTTGGGA GGTCAGAGGA GCCTGGTGAA CAAATGGACA 
ACTTTCCTGA AGGCCCGACT TGTGTGTAGC GTACCTGGGA ATGAAATAGG AGGGGACACA 
CATTTTGATG AGCTCCGGGA TGTCTTCCTT TTACAAACTC GGGACAGAAG AAATCCTCTC 
ATTTATGCTT TGTTTTCCAC ATCAAGCTCT GTCTTCCGTG GCTCTGCCAT ATGTGTCTAC 
ACCATGAATG ATGTGCGTAG AGCTTTCCTG GGACCCTTTG CTCATAAGGA AGGACCTAAC 
TATCAGTGGG TGTCATATCA GGGCAAGGTG CCATATCCTC GTCCCGGGAT GTGTCCCAGT 
AAGACATTTG GAAGCTTTGA GTCCACAAAG AGTTTCCCAG ATGATGTCCT TCAGTTTGCT 
CGGAATCACC CACTCATGTT CAACCCTGTG GTACCTGGGT CGGGCAGGCC TGTCTTCTTG 
CGCAGTCATG GAGACCTCAG TTTCACGCAG CTGGCTGTGG ACAGAGTGGC TGCTGCTGAT 
GGAGAGTATG ATGTCATGTT TATTGGGACA GATGTTGGTT CAGTGCTGAA AGTGATATCA 
GTGCCCAAGC AAAGCTGGCA TCACGTGGAA CAAGTTGTAC TGGAGGAGCT ACAAGTCTTC 
AAGAATGCAT CTCCTATAAC TACCATGCAG ATCTCCTCCA AGCGGCAACA GATATACGTG 
GGATCTGCAA GTGGCATCTC CCAGCTGCCC CTGCACCGCT GTGGCATCTA TGGCAAGGCA 
TGTGCAGAGT GTTGCTTGGC CAGGGATCCC TATTGTGCTT GGGATGGTGC CAGCTGTACA 
CGTTTTCTGC CCAACACTAA GAGGAGATCT CGTCGCCAAG ATGTGAGGAA TGGAGACCCC 
AACACCCTTT GTTCAGGAGA GCAGCAGAGA TTTAGCGTGC AGGAGAAACG TCTGTTGGGG 
GTTGAAGGGA GCAGCATATT CCTTGAGTGT CTTCCAAAAT CTCTACGTGC TCAAGTCACT 
TGGACATATC AGAAGGCACC AGAATCTTCA AGAACAGAGG TTCAGTTTGA TGAGCGTGTG 
CTTCAGACAG ACCGTGGTAT TGTTGTACGC AATGTATTAC GCCGTGATGC AGGCACCTTC 
ATATGTTACT CTTCAGAACA TGGATTTACA CAAACCCTTC TCCGCCTTCA CTTAGAGGTT 
ATTCCTTCCT CCAGGACTGA AGAACACTTA GGGCTCCAGC GTGTTCCGCA CAATCGATGG 
TACCAGGATT TCATGCGTCT AGTACAGCAG CCAGGCTCTG TGGAGCAAAT GTGCGAGGAG 
ATATGGACCC GCAAGGGCCG AACCTCACAT CCACCTGGAC CCCAGGGGGC CCCTCATGTT 
CCAGGTTCCC AAGGACGAAG TCTCCCAGCT GTGCCACCCC TCAATATTAA AACCAAAGCT 
CAAAAGTGGA AGCACTTAGA GGATAAGAGG AAGGTGCGGA GCCGCCGGAC GCATGAGCAG 
CTTAGCCGGG CTGAACGTGG TCCCAGAAGT GCCCCAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3b ( XM_012964329.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819783.1 Xenopus tropicalis semaphorin 3B (sema3b), transcript variant X2, mRNA.

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CloneID	OXa32103
Clone ID Related Accession (Same CDS sequence)	XM_012964329.2 , XM_031899870.1 , XM_012964329.3
Accession Version	XM_012964329.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	semaphorin-3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964329.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTCC AGCGTAGCCT CCTTTCTCTG TGCCTTCTGC TGTATTTTGG CCTCATTGGG 
AGCAACAAGG TCACGCCCCG GCTGAAATTG TCCTATCAGG ATCTGATGTC TCTGCATGGA 
CTCAGGACAT TTACACTAGA GCGCTCTTGC TGTTATGACG CACTCTTCCT AGATGAGGAG 
CGTGGCCGTC TCTTTGTGGG GGGGAAAAAC TACCTGATGT CTCTCAGCCT GGATAATATC 
AGCAAACAAG AACTGGCAAT TCACTGGCCG GCACCGGTGG AGTGGAGAGA AGAGTGTAAC 
TGGGCTGGAA AGGATATCAA TATAGACTGT ATGAACTACG TGAAGATCCT GCAGCCGTAC 
AACCGGACTC ATTTGTTCAC CTGTGGGACT GGAGCTTTCC ATCCAACTTG TGGATTTGTC 
CATGTTGGGC AGCGAACAGA TGACACAGTA TTTAAAATGG ATCTGAGAAG GATGGAGGAC 
GGGAAGGGGC GCTGTCCATA TGACCCCAAA CATATAGCAG CTGCCGTACT AGTAGGAGAT 
GAACTATATT CTGGAGTTGC AACGGACCTT ATGGGCCGGG ATTTCACCAT ATTTCGCAGC 
CTGGGTAGGC GCCCTCCCTT GCGCACAGAG CAGCATGATT CCCGTTGGCT AAACGAACCC 
AAGTTTGTGA AAGTTTTCTG GATTCCTGAG AGTGAAAATC CAGATGACGA TAAAATCTAC 
TTCTTCTTCA AGGAGACACC AGTGGAAGGA GCCGGTGGAG TGAAGCAGTC GTACGCTCGC 
ATTGGGCAAA TCTGCAGGAA TGACTTGGGA GGTCAGAGGA GCCTGGTGAA CAAATGGACA 
ACTTTCCTGA AGGCCCGACT TGTGTGTAGC GTACCTGGGA ATGAAATAGG AGGGGACACA 
CATTTTGATG AGCTCCGGGA TGTCTTCCTT TTACAAACTC GGGACAGAAG AAATCCTCTC 
ATTTATGCTT TGTTTTCCAC ATCAAGCTCT GTCTTCCGTG GCTCTGCCAT ATGTGTCTAC 
ACCATGAATG ATGTGCGTAG AGCTTTCCTG GGACCCTTTG CTCATAAGGA AGGACCTAAC 
TATCAGTGGG TGTCATATCA GGGCAAGGTG CCATATCCTC GTCCCGGGAT GTGTCCCAGT 
AAGACATTTG GAAGCTTTGA GTCCACAAAG AGTTTCCCAG ATGATGTCCT TCAGTTTGCT 
CGGAATCACC CACTCATGTT CAACCCTGTG GTACCTGGGT CGGGCAGGCC TGTCTTCTTG 
CGCAGTCATG GAGACCTCAG TTTCACGCAG CTGGCTGTGG ACAGAGTGGC TGCTGCTGAT 
GGAGAGTATG ATGTCATGTT TATTGGGACA GATGTTGGTT CAGTGCTGAA AGTGATATCA 
GTGCCCAAGC AAAGCTGGCA TCACGTGGAA CAAGTTGTAC TGGAGGAGCT ACAAGTCTTC 
AAGAATGCAT CTCCTATAAC TACCATGCAG ATCTCCTCCA AGCGGCAACA GATATACGTG 
GGATCTGCAA GTGGCATCTC CCAGCTGCCC CTGCACCGCT GTGGCATCTA TGGCAAGGCA 
TGTGCAGAGT GTTGCTTGGC CAGGGATCCC TATTGTGCTT GGGATGGTGC CAGCTGTACA 
CGTTTTCTGC CCAACACTAA GAGGAGATCT CGTCGCCAAG ATGTGAGGAA TGGAGACCCC 
AACACCCTTT GTTCAGGAGA GCAGCAGAGA TTTAGCGTGC AGGAGAAACG TCTGTTGGGG 
GTTGAAGGGA GCAGCATATT CCTTGAGTGT CTTCCAAAAT CTCTACGTGC TCAAGTCACT 
TGGACATATC AGAAGGCACC AGAATCTTCA AGAACAGAGG TTCAGTTTGA TGAGCGTGTG 
CTTCAGACAG ACCGTGGTAT TGTTGTACGC AATGTATTAC GCCGTGATGC AGGCACCTTC 
ATATGTTACT CTTCAGAACA TGGATTTACA CAAACCCTTC TCCGCCTTCA CTTAGAGGTT 
ATTCCTTCCT CCAGGACTGA AGAACACTTA GGGCTCCAGC GTGTTCCGCA CAATCGATGG 
TACCAGGATT TCATGCGTCT AGTACAGCAG CCAGGCTCTG TGGAGCAAAT GTGCGAGGAG 
ATATGGACCC GCAAGGGCCG AACCTCACAT CCACCTGGAC CCCAGGGGGC CCCTCATGTT 
CCAGGTTCCC AAGGACGAAG TCTCCCAGCT GTGCCACCCC TCAATATTAA AACCAAAGCT 
CAAAAGTGGA AGCACTTAGA GGATAAGAGG AAGGTGCGGA GCCGCCGGAC GCATGAGCAG 
CTTAGCCGGG CTGAACGTGG TCCCAGAAGT GCCCCAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819783.1
CDS	135..2456
Translation	MKLQRSLLSLCLLLYFGLIGSNKVTPRLKLSYQDLMSLHGLRTFTLERSCCYDALFLDEERGRLFVGGKNYLMSLSLDNISKQELAIHWPAPVEWREECNWAGKDINIDCMNYVKILQPYNRTHLFTCGTGAFHPTCGFVHVGQRTDDTVFKMDLRRMEDGKGRCPYDPKHIAAAVLVGDELYSGVATDLMGRDFTIFRSLGRRPPLRTEQHDSRWLNEPKFVKVFWIPESENPDDDKIYFFFKETPVEGAGGVKQSYARIGQICRNDLGGQRSLVNKWTTFLKARLVCSVPGNEIGGDTHFDELRDVFLLQTRDRRNPLIYALFSTSSSVFRGSAICVYTMNDVRRAFLGPFAHKEGPNYQWVSYQGKVPYPRPGMCPSKTFGSFESTKSFPDDVLQFARNHPLMFNPVVPGSGRPVFLRSHGDLSFTQLAVDRVAAADGEYDVMFIGTDVGSVLKVISVPKQSWHHVEQVVLEELQVFKNASPITTMQISSKRQQIYVGSASGISQLPLHRCGIYGKACAECCLARDPYCAWDGASCTRFLPNTKRRSRRQDVRNGDPNTLCSGEQQRFSVQEKRLLGVEGSSIFLECLPKSLRAQVTWTYQKAPESSRTEVQFDERVLQTDRGIVVRNVLRRDAGTFICYSSEHGFTQTLLRLHLEVIPSSRTEEHLGLQRVPHNRWYQDFMRLVQQPGSVEQMCEEIWTRKGRTSHPPGPQGAPHVPGSQGRSLPAVPPLNIKTKAQKWKHLEDKRKVRSRRTHEQLSRAERGPRSAPS

Target ORF information:

RefSeq Version	XM_012964329.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis semaphorin 3B (sema3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964329.3

ORF Insert Sequence:

ATGAAACTCC AGCGTAGCCT CCTTTCTCTG TGCCTTCTGC TGTATTTTGG CCTCATTGGG 
AGCAACAAGG TCACGCCCCG GCTGAAATTG TCCTATCAGG ATCTGATGTC TCTGCATGGA 
CTCAGGACAT TTACACTAGA GCGCTCTTGC TGTTATGACG CACTCTTCCT AGATGAGGAG 
CGTGGCCGTC TCTTTGTGGG GGGGAAAAAC TACCTGATGT CTCTCAGCCT GGATAATATC 
AGCAAACAAG AACTGGCAAT TCACTGGCCG GCACCGGTGG AGTGGAGAGA AGAGTGTAAC 
TGGGCTGGAA AGGATATCAA TATAGACTGT ATGAACTACG TGAAGATCCT GCAGCCGTAC 
AACCGGACTC ATTTGTTCAC CTGTGGGACT GGAGCTTTCC ATCCAACTTG TGGATTTGTC 
CATGTTGGGC AGCGAACAGA TGACACAGTA TTTAAAATGG ATCTGAGAAG GATGGAGGAC 
GGGAAGGGGC GCTGTCCATA TGACCCCAAA CATATAGCAG CTGCCGTACT AGTAGGAGAT 
GAACTATATT CTGGAGTTGC AACGGACCTT ATGGGCCGGG ATTTCACCAT ATTTCGCAGC 
CTGGGTAGGC GCCCTCCCTT GCGCACAGAG CAGCATGATT CCCGTTGGCT AAACGAACCC 
AAGTTTGTGA AAGTTTTCTG GATTCCTGAG AGTGAAAATC CAGATGACGA TAAAATCTAC 
TTCTTCTTCA AGGAGACACC AGTGGAAGGA GCCGGTGGAG TGAAGCAGTC GTACGCTCGC 
ATTGGGCAAA TCTGCAGGAA TGACTTGGGA GGTCAGAGGA GCCTGGTGAA CAAATGGACA 
ACTTTCCTGA AGGCCCGACT TGTGTGTAGC GTACCTGGGA ATGAAATAGG AGGGGACACA 
CATTTTGATG AGCTCCGGGA TGTCTTCCTT TTACAAACTC GGGACAGAAG AAATCCTCTC 
ATTTATGCTT TGTTTTCCAC ATCAAGCTCT GTCTTCCGTG GCTCTGCCAT ATGTGTCTAC 
ACCATGAATG ATGTGCGTAG AGCTTTCCTG GGACCCTTTG CTCATAAGGA AGGACCTAAC 
TATCAGTGGG TGTCATATCA GGGCAAGGTG CCATATCCTC GTCCCGGGAT GTGTCCCAGT 
AAGACATTTG GAAGCTTTGA GTCCACAAAG AGTTTCCCAG ATGATGTCCT TCAGTTTGCT 
CGGAATCACC CACTCATGTT CAACCCTGTG GTACCTGGGT CGGGCAGGCC TGTCTTCTTG 
CGCAGTCATG GAGACCTCAG TTTCACGCAG CTGGCTGTGG ACAGAGTGGC TGCTGCTGAT 
GGAGAGTATG ATGTCATGTT TATTGGGACA GATGTTGGTT CAGTGCTGAA AGTGATATCA 
GTGCCCAAGC AAAGCTGGCA TCACGTGGAA CAAGTTGTAC TGGAGGAGCT ACAAGTCTTC 
AAGAATGCAT CTCCTATAAC TACCATGCAG ATCTCCTCCA AGCGGCAACA GATATACGTG 
GGATCTGCAA GTGGCATCTC CCAGCTGCCC CTGCACCGCT GTGGCATCTA TGGCAAGGCA 
TGTGCAGAGT GTTGCTTGGC CAGGGATCCC TATTGTGCTT GGGATGGTGC CAGCTGTACA 
CGTTTTCTGC CCAACACTAA GAGGAGATCT CGTCGCCAAG ATGTGAGGAA TGGAGACCCC 
AACACCCTTT GTTCAGGAGA GCAGCAGAGA TTTAGCGTGC AGGAGAAACG TCTGTTGGGG 
GTTGAAGGGA GCAGCATATT CCTTGAGTGT CTTCCAAAAT CTCTACGTGC TCAAGTCACT 
TGGACATATC AGAAGGCACC AGAATCTTCA AGAACAGAGG TTCAGTTTGA TGAGCGTGTG 
CTTCAGACAG ACCGTGGTAT TGTTGTACGC AATGTATTAC GCCGTGATGC AGGCACCTTC 
ATATGTTACT CTTCAGAACA TGGATTTACA CAAACCCTTC TCCGCCTTCA CTTAGAGGTT 
ATTCCTTCCT CCAGGACTGA AGAACACTTA GGGCTCCAGC GTGTTCCGCA CAATCGATGG 
TACCAGGATT TCATGCGTCT AGTACAGCAG CCAGGCTCTG TGGAGCAAAT GTGCGAGGAG 
ATATGGACCC GCAAGGGCCG AACCTCACAT CCACCTGGAC CCCAGGGGGC CCCTCATGTT 
CCAGGTTCCC AAGGACGAAG TCTCCCAGCT GTGCCACCCC TCAATATTAA AACCAAAGCT 
CAAAAGTGGA AGCACTTAGA GGATAAGAGG AAGGTGCGGA GCCGCCGGAC GCATGAGCAG 
CTTAGCCGGG CTGAACGTGG TCCCAGAAGT GCCCCAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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sema3b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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