pkmyt1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pkmyt1
Entrez Gene ID	100145798
Full Name	protein kinase, membrane associated tyrosine/threonine 1
General protein information	Preferred Names protein kinase, membrane associated tyrosine/threonine 1 Names membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012970107.2	XP_012825561.1	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X3
XM_012970107.3	XP_012825561.1	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X3
XM_012970105.2	XP_012825559.1	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X1
XM_012970106.2	XP_012825560.1	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X2
NM_001127159.1	NP_001120631.1	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
XM_012970106.3	XP_012825560.1	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X2
XM_012970105.3	XP_012825559.1	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PKMYT1	XP_005621713.1
Mus musculus (house mouse)	Pkmyt1	NP_075545.2
Homo sapiens (human)	PKMYT1	NP_004194.3
Rattus norvegicus (Norway rat)	Pkmyt1	NP_001099236.1
Danio rerio (zebrafish)	pkmyt1	NP_001091666.1
Xenopus tropicalis (tropical clawed frog)	pkmyt1	NP_001120631.1
Pan troglodytes (chimpanzee)	PKMYT1	XP_001164962.1
Bos taurus (cattle)	PKMYT1	XP_005224692.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pkmyt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pkmyt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa01536	NM_001127159.1 Latest version!	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa01536	XM_012970107.3 Latest version!	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa32054	XM_012970105.3 Latest version!	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa32055	XM_012970106.3 Latest version!	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OXa01536	XM_012970107.2	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa32055	XM_012970106.2	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa32054	XM_012970105.2	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pkmyt1 ( NM_001127159.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001120631.1 Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), mRNA.

Price & Availability↑

CloneID	OXa01536
Clone ID Related Accession (Same CDS sequence)	XM_012970107.2 , XM_012970107.3 , NM_001127159.1
Accession Version	NM_001127159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161784.1. ##Evidence-Data-START## Transcript exon combination :: BC161784.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGCCTGTTC CAGGAGATGA CATGGGAGAT ACTCCCCTTA CTCGCACCCC TATCCCTGTG 
CCTGCTCACT TCAGTCAGGC AGAGCAAAGC TTTTCACTTA AAAAAAGAGG GCGCTCCCTT 
TGCTATACCC TGCCACCCAG ACCTCCAGTT AAGAGCGCTC TGCCTATTAG CCGCCTATTC 
CCGAACAAAC AGCGGTCTTG GAGCCAGCCT AGGCCACAAA GTGTTTCTTT CCGAAGTCCC 
CAGAACAATC CCCCTGCAAG CGAGCTCTAT GACCAGAGCA AAGGAGAGAC CTTTTTCAAG 
CAGTGTTTTA GAAGCATTTG CAAATTAGGC CGAGGATCCT TTGGCGAGGT ATACAAGGTG 
CAAAGCCTTG AGGATGGATG CTTCTATGCC GTGAAGCGCT CAGTTTCTCC TTTCCGTGGC 
GAGTCAGACC GCCAACGGAA GCTTCAGGAG GTGAGGAAGC ATGAGAGAGT CGGGGTGCAC 
CCCAACTGCC TGCGTTTTGT GCGAGCATGG GAAGAGAAAC GATTGCTCTA CCTGCAGACG 
GAGTTATGTG CAGGGAGTCT GCAACAGCAC TCGGAAGAAT TTTCTGGGCC CCTCCCTCCA 
CGTAAAATAT GGAACATAAC TTGTGACCTG TTGCACGGTC TCAAACATCT TCATGATCGC 
AACCTTCTGC ACCTTGACAT TAAACCAGCT AACGTCTTCA TTTCATTTTC TGGTGTTTGC 
AAACTGGGTG ACTTTGGACT CATGGTAGAA TTAGATGGAA CCGAAGGAAG TGGAGAAGCG 
CAGGAAGGTG ACCCTCGATA CATGGCACCT GAACTCTTGG ATGGGATCTT TTCGAAGGCC 
GCTGATGTGT TCAGCCTTGG AATGAGTCTT CTAGAGGTCG CTTGCAATAT GGAGCTTCCA 
AAAAGTGGAG ACGGCTGGCA GCAGCTAAGA CAAGGGCGTC TCCCTACAGA GTTCACATCA 
GACCTGCCGC CAGACTTTCT GAAAGTTCTT GCTGCAATGC TGGAGCCTGA CTACCGCCGG 
CGTGCCACAG TGGACTGGCT GCTCTCCCTT CCTGCTATCC GTAATGCAGA GAGATGGAGG 
ATGGTGACAC TAGCTCAGGA AAGGACACTT GGAAAGATAA TTACAGTCTA TCAGTTCATC 
GTTTGGCTTG TATCTATCGT GTTTCAAGGT CTCAACCGCC CAGTTGTAGG ATTTTTACAT 
TACTGTGGAT TAAGAGCACT TCCAAGGTCC CCTCCCTGTT CTCCTTCTCC CAACCTAGGG 
GAGAGCAGCT TCTCTAGTGA CTGGGATGAT GAGAGTCTTG GTGACGATGT GTTTGAGGTT 
CCACCCAGCC CATTGGCCGC TCACCGGAAC TTTACATACC ATGGGCAGGA GCTTATTGGC 
AGACATTCCC CAGACCTGCT CTCAAGACCA TCACTTGGAA GCACCTCTAC CCCTCGGAAT 
TTGTCTCCTG AATTCAGCAT GAGAAAGAGG TCTGCTTTGC CTCTAACTCC TAATGTCAGC 
CGGATTAGTC AGGAGTCTTC AGGCAGTGTG AAGTCTACTA GCCCCTCCAC CAGTCATAGT 
TCCTCTGGAT TTGTCGATGC CGAGGTCCAA CGTTCTTCAT TTCTCCCTCG CAATCTGCTC 
AGCATGTTTG ACGATGCCAC CGAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120631.1
CDS	102..1751
Translation	MPVPGDDMGDTPLTRTPIPVPAHFSQAEQSFSLKKRGRSLCYTLPPRPPVKSALPISRLFPNKQRSWSQPRPQSVSFRSPQNNPPASELYDQSKGETFFKQCFRSICKLGRGSFGEVYKVQSLEDGCFYAVKRSVSPFRGESDRQRKLQEVRKHERVGVHPNCLRFVRAWEEKRLLYLQTELCAGSLQQHSEEFSGPLPPRKIWNITCDLLHGLKHLHDRNLLHLDIKPANVFISFSGVCKLGDFGLMVELDGTEGSGEAQEGDPRYMAPELLDGIFSKAADVFSLGMSLLEVACNMELPKSGDGWQQLRQGRLPTEFTSDLPPDFLKVLAAMLEPDYRRRATVDWLLSLPAIRNAERWRMVTLAQERTLGKIITVYQFIVWLVSIVFQGLNRPVVGFLHYCGLRALPRSPPCSPSPNLGESSFSSDWDDESLGDDVFEVPPSPLAAHRNFTYHGQELIGRHSPDLLSRPSLGSTSTPRNLSPEFSMRKRSALPLTPNVSRISQESSGSVKSTSPSTSHSSSGFVDAEVQRSSFLPRNLLSMFDDATEQ

Target ORF information:

RefSeq Version	NM_001127159.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127159.1

ORF Insert Sequence:

ATGCCTGTTC CAGGAGATGA CATGGGAGAT ACTCCCCTTA CTCGCACCCC TATCCCTGTG 
CCTGCTCACT TCAGTCAGGC AGAGCAAAGC TTTTCACTTA AAAAAAGAGG GCGCTCCCTT 
TGCTATACCC TGCCACCCAG ACCTCCAGTT AAGAGCGCTC TGCCTATTAG CCGCCTATTC 
CCGAACAAAC AGCGGTCTTG GAGCCAGCCT AGGCCACAAA GTGTTTCTTT CCGAAGTCCC 
CAGAACAATC CCCCTGCAAG CGAGCTCTAT GACCAGAGCA AAGGAGAGAC CTTTTTCAAG 
CAGTGTTTTA GAAGCATTTG CAAATTAGGC CGAGGATCCT TTGGCGAGGT ATACAAGGTG 
CAAAGCCTTG AGGATGGATG CTTCTATGCC GTGAAGCGCT CAGTTTCTCC TTTCCGTGGC 
GAGTCAGACC GCCAACGGAA GCTTCAGGAG GTGAGGAAGC ATGAGAGAGT CGGGGTGCAC 
CCCAACTGCC TGCGTTTTGT GCGAGCATGG GAAGAGAAAC GATTGCTCTA CCTGCAGACG 
GAGTTATGTG CAGGGAGTCT GCAACAGCAC TCGGAAGAAT TTTCTGGGCC CCTCCCTCCA 
CGTAAAATAT GGAACATAAC TTGTGACCTG TTGCACGGTC TCAAACATCT TCATGATCGC 
AACCTTCTGC ACCTTGACAT TAAACCAGCT AACGTCTTCA TTTCATTTTC TGGTGTTTGC 
AAACTGGGTG ACTTTGGACT CATGGTAGAA TTAGATGGAA CCGAAGGAAG TGGAGAAGCG 
CAGGAAGGTG ACCCTCGATA CATGGCACCT GAACTCTTGG ATGGGATCTT TTCGAAGGCC 
GCTGATGTGT TCAGCCTTGG AATGAGTCTT CTAGAGGTCG CTTGCAATAT GGAGCTTCCA 
AAAAGTGGAG ACGGCTGGCA GCAGCTAAGA CAAGGGCGTC TCCCTACAGA GTTCACATCA 
GACCTGCCGC CAGACTTTCT GAAAGTTCTT GCTGCAATGC TGGAGCCTGA CTACCGCCGG 
CGTGCCACAG TGGACTGGCT GCTCTCCCTT CCTGCTATCC GTAATGCAGA GAGATGGAGG 
ATGGTGACAC TAGCTCAGGA AAGGACACTT GGAAAGATAA TTACAGTCTA TCAGTTCATC 
GTTTGGCTTG TATCTATCGT GTTTCAAGGT CTCAACCGCC CAGTTGTAGG ATTTTTACAT 
TACTGTGGAT TAAGAGCACT TCCAAGGTCC CCTCCCTGTT CTCCTTCTCC CAACCTAGGG 
GAGAGCAGCT TCTCTAGTGA CTGGGATGAT GAGAGTCTTG GTGACGATGT GTTTGAGGTT 
CCACCCAGCC CATTGGCCGC TCACCGGAAC TTTACATACC ATGGGCAGGA GCTTATTGGC 
AGACATTCCC CAGACCTGCT CTCAAGACCA TCACTTGGAA GCACCTCTAC CCCTCGGAAT 
TTGTCTCCTG AATTCAGCAT GAGAAAGAGG TCTGCTTTGC CTCTAACTCC TAATGTCAGC 
CGGATTAGTC AGGAGTCTTC AGGCAGTGTG AAGTCTACTA GCCCCTCCAC CAGTCATAGT 
TCCTCTGGAT TTGTCGATGC CGAGGTCCAA CGTTCTTCAT TTCTCCCTCG CAATCTGCTC 
AGCATGTTTG ACGATGCCAC CGAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkmyt1 ( XM_012970107.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825561.1 Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa01536
Clone ID Related Accession (Same CDS sequence)	XM_012970107.2 , XM_012970107.3 , NM_001127159.1
Accession Version	XM_012970107.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTTC CAGGAGATGA CATGGGAGAT ACTCCCCTTA CTCGCACCCC TATCCCTGTG 
CCTGCTCACT TCAGTCAGGC AGAGCAAAGC TTTTCACTTA AAAAAAGAGG GCGCTCCCTT 
TGCTATACCC TGCCACCCAG ACCTCCAGTT AAGAGCGCTC TGCCTATTAG CCGCCTATTC 
CCGAACAAAC AGCGGTCTTG GAGCCAGCCT AGGCCACAAA GTGTTTCTTT CCGAAGTCCC 
CAGAACAATC CCCCTGCAAG CGAGCTCTAT GACCAGAGCA AAGGAGAGAC CTTTTTCAAG 
CAGTGTTTTA GAAGCATTTG CAAATTAGGC CGAGGATCCT TTGGCGAGGT ATACAAGGTG 
CAAAGCCTTG AGGATGGATG CTTCTATGCC GTGAAGCGCT CAGTTTCTCC TTTCCGTGGC 
GAGTCAGACC GCCAACGGAA GCTTCAGGAG GTGAGGAAGC ATGAGAGAGT CGGGGTGCAC 
CCCAACTGCC TGCGTTTTGT GCGAGCATGG GAAGAGAAAC GATTGCTCTA CCTGCAGACG 
GAGTTATGTG CAGGGAGTCT GCAACAGCAC TCGGAAGAAT TTTCTGGGCC CCTCCCTCCA 
CGTAAAATAT GGAACATAAC TTGTGACCTG TTGCACGGTC TCAAACATCT TCATGATCGC 
AACCTTCTGC ACCTTGACAT TAAACCAGCT AACGTCTTCA TTTCATTTTC TGGTGTTTGC 
AAACTGGGTG ACTTTGGACT CATGGTAGAA TTAGATGGAA CCGAAGGAAG TGGAGAAGCG 
CAGGAAGGTG ACCCTCGATA CATGGCACCT GAACTCTTGG ATGGGATCTT TTCGAAGGCC 
GCTGATGTGT TCAGCCTTGG AATGAGTCTT CTAGAGGTCG CTTGCAATAT GGAGCTTCCA 
AAAAGTGGAG ACGGCTGGCA GCAGCTAAGA CAAGGGCGTC TCCCTACAGA GTTCACATCA 
GACCTGCCGC CAGACTTTCT GAAAGTTCTT GCTGCAATGC TGGAGCCTGA CTACCGCCGG 
CGTGCCACAG TGGACTGGCT GCTCTCCCTT CCTGCTATCC GTAATGCAGA GAGATGGAGG 
ATGGTGACAC TAGCTCAGGA AAGGACACTT GGAAAGATAA TTACAGTCTA TCAGTTCATC 
GTTTGGCTTG TATCTATCGT GTTTCAAGGT CTCAACCGCC CAGTTGTAGG ATTTTTACAT 
TACTGTGGAT TAAGAGCACT TCCAAGGTCC CCTCCCTGTT CTCCTTCTCC CAACCTAGGG 
GAGAGCAGCT TCTCTAGTGA CTGGGATGAT GAGAGTCTTG GTGACGATGT GTTTGAGGTT 
CCACCCAGCC CATTGGCCGC TCACCGGAAC TTTACATACC ATGGGCAGGA GCTTATTGGC 
AGACATTCCC CAGACCTGCT CTCAAGACCA TCACTTGGAA GCACCTCTAC CCCTCGGAAT 
TTGTCTCCTG AATTCAGCAT GAGAAAGAGG TCTGCTTTGC CTCTAACTCC TAATGTCAGC 
CGGATTAGTC AGGAGTCTTC AGGCAGTGTG AAGTCTACTA GCCCCTCCAC CAGTCATAGT 
TCCTCTGGAT TTGTCGATGC CGAGGTCCAA CGTTCTTCAT TTCTCCCTCG CAATCTGCTC 
AGCATGTTTG ACGATGCCAC CGAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825561.1
CDS	964..2613
Translation	MPVPGDDMGDTPLTRTPIPVPAHFSQAEQSFSLKKRGRSLCYTLPPRPPVKSALPISRLFPNKQRSWSQPRPQSVSFRSPQNNPPASELYDQSKGETFFKQCFRSICKLGRGSFGEVYKVQSLEDGCFYAVKRSVSPFRGESDRQRKLQEVRKHERVGVHPNCLRFVRAWEEKRLLYLQTELCAGSLQQHSEEFSGPLPPRKIWNITCDLLHGLKHLHDRNLLHLDIKPANVFISFSGVCKLGDFGLMVELDGTEGSGEAQEGDPRYMAPELLDGIFSKAADVFSLGMSLLEVACNMELPKSGDGWQQLRQGRLPTEFTSDLPPDFLKVLAAMLEPDYRRRATVDWLLSLPAIRNAERWRMVTLAQERTLGKIITVYQFIVWLVSIVFQGLNRPVVGFLHYCGLRALPRSPPCSPSPNLGESSFSSDWDDESLGDDVFEVPPSPLAAHRNFTYHGQELIGRHSPDLLSRPSLGSTSTPRNLSPEFSMRKRSALPLTPNVSRISQESSGSVKSTSPSTSHSSSGFVDAEVQRSSFLPRNLLSMFDDATEQ

Target ORF information:

RefSeq Version	XM_012970107.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970107.2

ORF Insert Sequence:

ATGCCTGTTC CAGGAGATGA CATGGGAGAT ACTCCCCTTA CTCGCACCCC TATCCCTGTG 
CCTGCTCACT TCAGTCAGGC AGAGCAAAGC TTTTCACTTA AAAAAAGAGG GCGCTCCCTT 
TGCTATACCC TGCCACCCAG ACCTCCAGTT AAGAGCGCTC TGCCTATTAG CCGCCTATTC 
CCGAACAAAC AGCGGTCTTG GAGCCAGCCT AGGCCACAAA GTGTTTCTTT CCGAAGTCCC 
CAGAACAATC CCCCTGCAAG CGAGCTCTAT GACCAGAGCA AAGGAGAGAC CTTTTTCAAG 
CAGTGTTTTA GAAGCATTTG CAAATTAGGC CGAGGATCCT TTGGCGAGGT ATACAAGGTG 
CAAAGCCTTG AGGATGGATG CTTCTATGCC GTGAAGCGCT CAGTTTCTCC TTTCCGTGGC 
GAGTCAGACC GCCAACGGAA GCTTCAGGAG GTGAGGAAGC ATGAGAGAGT CGGGGTGCAC 
CCCAACTGCC TGCGTTTTGT GCGAGCATGG GAAGAGAAAC GATTGCTCTA CCTGCAGACG 
GAGTTATGTG CAGGGAGTCT GCAACAGCAC TCGGAAGAAT TTTCTGGGCC CCTCCCTCCA 
CGTAAAATAT GGAACATAAC TTGTGACCTG TTGCACGGTC TCAAACATCT TCATGATCGC 
AACCTTCTGC ACCTTGACAT TAAACCAGCT AACGTCTTCA TTTCATTTTC TGGTGTTTGC 
AAACTGGGTG ACTTTGGACT CATGGTAGAA TTAGATGGAA CCGAAGGAAG TGGAGAAGCG 
CAGGAAGGTG ACCCTCGATA CATGGCACCT GAACTCTTGG ATGGGATCTT TTCGAAGGCC 
GCTGATGTGT TCAGCCTTGG AATGAGTCTT CTAGAGGTCG CTTGCAATAT GGAGCTTCCA 
AAAAGTGGAG ACGGCTGGCA GCAGCTAAGA CAAGGGCGTC TCCCTACAGA GTTCACATCA 
GACCTGCCGC CAGACTTTCT GAAAGTTCTT GCTGCAATGC TGGAGCCTGA CTACCGCCGG 
CGTGCCACAG TGGACTGGCT GCTCTCCCTT CCTGCTATCC GTAATGCAGA GAGATGGAGG 
ATGGTGACAC TAGCTCAGGA AAGGACACTT GGAAAGATAA TTACAGTCTA TCAGTTCATC 
GTTTGGCTTG TATCTATCGT GTTTCAAGGT CTCAACCGCC CAGTTGTAGG ATTTTTACAT 
TACTGTGGAT TAAGAGCACT TCCAAGGTCC CCTCCCTGTT CTCCTTCTCC CAACCTAGGG 
GAGAGCAGCT TCTCTAGTGA CTGGGATGAT GAGAGTCTTG GTGACGATGT GTTTGAGGTT 
CCACCCAGCC CATTGGCCGC TCACCGGAAC TTTACATACC ATGGGCAGGA GCTTATTGGC 
AGACATTCCC CAGACCTGCT CTCAAGACCA TCACTTGGAA GCACCTCTAC CCCTCGGAAT 
TTGTCTCCTG AATTCAGCAT GAGAAAGAGG TCTGCTTTGC CTCTAACTCC TAATGTCAGC 
CGGATTAGTC AGGAGTCTTC AGGCAGTGTG AAGTCTACTA GCCCCTCCAC CAGTCATAGT 
TCCTCTGGAT TTGTCGATGC CGAGGTCCAA CGTTCTTCAT TTCTCCCTCG CAATCTGCTC 
AGCATGTTTG ACGATGCCAC CGAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkmyt1 ( XM_012970105.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825559.1 Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa32054
Clone ID Related Accession (Same CDS sequence)	XM_012970105.2 , XM_012970105.3
Accession Version	XM_012970105.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCGCA ACCGCGCAGC CGCGCTTGGA GGGGCGGGCC GCCATCTTGT GTGTGGCATA 
CCTGGGATAG TGGGCGGCGC TGCTTCCCCA CCTAAGTCTC CTGTATCTCC GAAGAGCGAA 
CAAGGACCGC CCTTACATTC ACCTTTAACG TGCCTGCTCA TTATGCCTGT TCCAGGAGAT 
GACATGGGAG ATACTCCCCT TACTCGCACC CCTATCCCTG TGCCTGCTCA CTTCAGTCAG 
GCAGAGCAAA GCTTTTCACT TAAAAAAAGA GGGCGCTCCC TTTGCTATAC CCTGCCACCC 
AGACCTCCAG TTAAGAGCGC TCTGCCTATT AGCCGCCTAT TCCCGAACAA ACAGCGGTCT 
TGGAGCCAGC CTAGGCCACA AAGTGTTTCT TTCCGAAGTC CCCAGAACAA TCCCCCTGCA 
AGCGAGCTCT ATGACCAGAG CAAAGGAGAG ACCTTTTTCA AGCAGTGTTT TAGAAGCATT 
TGCAAATTAG GCCGAGGATC CTTTGGCGAG GTATACAAGG TGCAAAGCCT TGAGGATGGA 
TGCTTCTATG CCGTGAAGCG CTCAGTTTCT CCTTTCCGTG GCGAGTCAGA CCGCCAACGG 
AAGCTTCAGG AGGTGAGGAA GCATGAGAGA GTCGGGGTGC ACCCCAACTG CCTGCGTTTT 
GTGCGAGCAT GGGAAGAGAA ACGATTGCTC TACCTGCAGA CGGAGTTATG TGCAGGGAGT 
CTGCAACAGC ACTCGGAAGA ATTTTCTGGG CCCCTCCCTC CACGTAAAAT ATGGAACATA 
ACTTGTGACC TGTTGCACGG TCTCAAACAT CTTCATGATC GCAACCTTCT GCACCTTGAC 
ATTAAACCAG CTAACGTCTT CATTTCATTT TCTGGTGTTT GCAAACTGGG TGACTTTGGA 
CTCATGGTAG AATTAGATGG AACCGAAGGA AGTGGAGAAG CGCAGGAAGG TGACCCTCGA 
TACATGGCAC CTGAACTCTT GGATGGGATC TTTTCGAAGG CCGCTGATGT GTTCAGCCTT 
GGAATGAGTC TTCTAGAGGT CGCTTGCAAT ATGGAGCTTC CAAAAAGTGG AGACGGCTGG 
CAGCAGCTAA GACAAGGGCG TCTCCCTACA GAGTTCACAT CAGACCTGCC GCCAGACTTT 
CTGAAAGTTC TTGCTGCAAT GCTGGAGCCT GACTACCGCC GGCGTGCCAC AGTGGACTGG 
CTGCTCTCCC TTCCTGCTAT CCGTAATGCA GAGAGATGGA GGATGGTGAC ACTAGCTCAG 
GAAAGGACAC TTGGAAAGAT AATTACAGTC TATCAGTTCA TCGTTTGGCT TGTATCTATC 
GTGTTTCAAG GTCTCAACCG CCCAGTTGTA GGATTTTTAC ATTACTGTGG ATTAAGAGCA 
CTTCCAAGGT CCCCTCCCTG TTCTCCTTCT CCCAACCTAG GGGAGAGCAG CTTCTCTAGT 
GACTGGGATG ATGAGAGTCT TGGTGACGAT GTGTTTGAGG TTCCACCCAG CCCATTGGCC 
GCTCACCGGA ACTTTACATA CCATGGGCAG GAGCTTATTG GCAGACATTC CCCAGACCTG 
CTCTCAAGAC CATCACTTGG AAGCACCTCT ACCCCTCGGA ATTTGTCTCC TGAATTCAGC 
ATGAGAAAGA GGTCTGCTTT GCCTCTAACT CCTAATGTCA GCCGGATTAG TCAGGAGTCT 
TCAGGCAGTG TGAAGTCTAC TAGCCCCTCC ACCAGTCATA GTTCCTCTGG ATTTGTCGAT 
GCCGAGGTCC AACGTTCTTC ATTTCTCCCT CGCAATCTGC TCAGCATGTT TGACGATGCC 
ACCGAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825559.1
CDS	25..1836
Translation	MHRNRAAALGGAGRHLVCGIPGIVGGAASPPKSPVSPKSEQGPPLHSPLTCLLIMPVPGDDMGDTPLTRTPIPVPAHFSQAEQSFSLKKRGRSLCYTLPPRPPVKSALPISRLFPNKQRSWSQPRPQSVSFRSPQNNPPASELYDQSKGETFFKQCFRSICKLGRGSFGEVYKVQSLEDGCFYAVKRSVSPFRGESDRQRKLQEVRKHERVGVHPNCLRFVRAWEEKRLLYLQTELCAGSLQQHSEEFSGPLPPRKIWNITCDLLHGLKHLHDRNLLHLDIKPANVFISFSGVCKLGDFGLMVELDGTEGSGEAQEGDPRYMAPELLDGIFSKAADVFSLGMSLLEVACNMELPKSGDGWQQLRQGRLPTEFTSDLPPDFLKVLAAMLEPDYRRRATVDWLLSLPAIRNAERWRMVTLAQERTLGKIITVYQFIVWLVSIVFQGLNRPVVGFLHYCGLRALPRSPPCSPSPNLGESSFSSDWDDESLGDDVFEVPPSPLAAHRNFTYHGQELIGRHSPDLLSRPSLGSTSTPRNLSPEFSMRKRSALPLTPNVSRISQESSGSVKSTSPSTSHSSSGFVDAEVQRSSFLPRNLLSMFDDATEQ

Target ORF information:

RefSeq Version	XM_012970105.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970105.2

ORF Insert Sequence:

ATGCACCGCA ACCGCGCAGC CGCGCTTGGA GGGGCGGGCC GCCATCTTGT GTGTGGCATA 
CCTGGGATAG TGGGCGGCGC TGCTTCCCCA CCTAAGTCTC CTGTATCTCC GAAGAGCGAA 
CAAGGACCGC CCTTACATTC ACCTTTAACG TGCCTGCTCA TTATGCCTGT TCCAGGAGAT 
GACATGGGAG ATACTCCCCT TACTCGCACC CCTATCCCTG TGCCTGCTCA CTTCAGTCAG 
GCAGAGCAAA GCTTTTCACT TAAAAAAAGA GGGCGCTCCC TTTGCTATAC CCTGCCACCC 
AGACCTCCAG TTAAGAGCGC TCTGCCTATT AGCCGCCTAT TCCCGAACAA ACAGCGGTCT 
TGGAGCCAGC CTAGGCCACA AAGTGTTTCT TTCCGAAGTC CCCAGAACAA TCCCCCTGCA 
AGCGAGCTCT ATGACCAGAG CAAAGGAGAG ACCTTTTTCA AGCAGTGTTT TAGAAGCATT 
TGCAAATTAG GCCGAGGATC CTTTGGCGAG GTATACAAGG TGCAAAGCCT TGAGGATGGA 
TGCTTCTATG CCGTGAAGCG CTCAGTTTCT CCTTTCCGTG GCGAGTCAGA CCGCCAACGG 
AAGCTTCAGG AGGTGAGGAA GCATGAGAGA GTCGGGGTGC ACCCCAACTG CCTGCGTTTT 
GTGCGAGCAT GGGAAGAGAA ACGATTGCTC TACCTGCAGA CGGAGTTATG TGCAGGGAGT 
CTGCAACAGC ACTCGGAAGA ATTTTCTGGG CCCCTCCCTC CACGTAAAAT ATGGAACATA 
ACTTGTGACC TGTTGCACGG TCTCAAACAT CTTCATGATC GCAACCTTCT GCACCTTGAC 
ATTAAACCAG CTAACGTCTT CATTTCATTT TCTGGTGTTT GCAAACTGGG TGACTTTGGA 
CTCATGGTAG AATTAGATGG AACCGAAGGA AGTGGAGAAG CGCAGGAAGG TGACCCTCGA 
TACATGGCAC CTGAACTCTT GGATGGGATC TTTTCGAAGG CCGCTGATGT GTTCAGCCTT 
GGAATGAGTC TTCTAGAGGT CGCTTGCAAT ATGGAGCTTC CAAAAAGTGG AGACGGCTGG 
CAGCAGCTAA GACAAGGGCG TCTCCCTACA GAGTTCACAT CAGACCTGCC GCCAGACTTT 
CTGAAAGTTC TTGCTGCAAT GCTGGAGCCT GACTACCGCC GGCGTGCCAC AGTGGACTGG 
CTGCTCTCCC TTCCTGCTAT CCGTAATGCA GAGAGATGGA GGATGGTGAC ACTAGCTCAG 
GAAAGGACAC TTGGAAAGAT AATTACAGTC TATCAGTTCA TCGTTTGGCT TGTATCTATC 
GTGTTTCAAG GTCTCAACCG CCCAGTTGTA GGATTTTTAC ATTACTGTGG ATTAAGAGCA 
CTTCCAAGGT CCCCTCCCTG TTCTCCTTCT CCCAACCTAG GGGAGAGCAG CTTCTCTAGT 
GACTGGGATG ATGAGAGTCT TGGTGACGAT GTGTTTGAGG TTCCACCCAG CCCATTGGCC 
GCTCACCGGA ACTTTACATA CCATGGGCAG GAGCTTATTG GCAGACATTC CCCAGACCTG 
CTCTCAAGAC CATCACTTGG AAGCACCTCT ACCCCTCGGA ATTTGTCTCC TGAATTCAGC 
ATGAGAAAGA GGTCTGCTTT GCCTCTAACT CCTAATGTCA GCCGGATTAG TCAGGAGTCT 
TCAGGCAGTG TGAAGTCTAC TAGCCCCTCC ACCAGTCATA GTTCCTCTGG ATTTGTCGAT 
GCCGAGGTCC AACGTTCTTC ATTTCTCCCT CGCAATCTGC TCAGCATGTT TGACGATGCC 
ACCGAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkmyt1 ( XM_012970106.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825560.1 Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X2, mRNA.

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CloneID	OXa32055
Clone ID Related Accession (Same CDS sequence)	XM_012970106.2 , XM_012970106.3
Accession Version	XM_012970106.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGGC GCCAATTTTC TCCTGTATCT CCGAAGAGCG AACAAGGACC GCCCTTACAT 
TCACCTTTAA CGTGCCTGCT CATTATGCCT GTTCCAGGAG ATGACATGGG AGATACTCCC 
CTTACTCGCA CCCCTATCCC TGTGCCTGCT CACTTCAGTC AGGCAGAGCA AAGCTTTTCA 
CTTAAAAAAA GAGGGCGCTC CCTTTGCTAT ACCCTGCCAC CCAGACCTCC AGTTAAGAGC 
GCTCTGCCTA TTAGCCGCCT ATTCCCGAAC AAACAGCGGT CTTGGAGCCA GCCTAGGCCA 
CAAAGTGTTT CTTTCCGAAG TCCCCAGAAC AATCCCCCTG CAAGCGAGCT CTATGACCAG 
AGCAAAGGAG AGACCTTTTT CAAGCAGTGT TTTAGAAGCA TTTGCAAATT AGGCCGAGGA 
TCCTTTGGCG AGGTATACAA GGTGCAAAGC CTTGAGGATG GATGCTTCTA TGCCGTGAAG 
CGCTCAGTTT CTCCTTTCCG TGGCGAGTCA GACCGCCAAC GGAAGCTTCA GGAGGTGAGG 
AAGCATGAGA GAGTCGGGGT GCACCCCAAC TGCCTGCGTT TTGTGCGAGC ATGGGAAGAG 
AAACGATTGC TCTACCTGCA GACGGAGTTA TGTGCAGGGA GTCTGCAACA GCACTCGGAA 
GAATTTTCTG GGCCCCTCCC TCCACGTAAA ATATGGAACA TAACTTGTGA CCTGTTGCAC 
GGTCTCAAAC ATCTTCATGA TCGCAACCTT CTGCACCTTG ACATTAAACC AGCTAACGTC 
TTCATTTCAT TTTCTGGTGT TTGCAAACTG GGTGACTTTG GACTCATGGT AGAATTAGAT 
GGAACCGAAG GAAGTGGAGA AGCGCAGGAA GGTGACCCTC GATACATGGC ACCTGAACTC 
TTGGATGGGA TCTTTTCGAA GGCCGCTGAT GTGTTCAGCC TTGGAATGAG TCTTCTAGAG 
GTCGCTTGCA ATATGGAGCT TCCAAAAAGT GGAGACGGCT GGCAGCAGCT AAGACAAGGG 
CGTCTCCCTA CAGAGTTCAC ATCAGACCTG CCGCCAGACT TTCTGAAAGT TCTTGCTGCA 
ATGCTGGAGC CTGACTACCG CCGGCGTGCC ACAGTGGACT GGCTGCTCTC CCTTCCTGCT 
ATCCGTAATG CAGAGAGATG GAGGATGGTG ACACTAGCTC AGGAAAGGAC ACTTGGAAAG 
ATAATTACAG TCTATCAGTT CATCGTTTGG CTTGTATCTA TCGTGTTTCA AGGTCTCAAC 
CGCCCAGTTG TAGGATTTTT ACATTACTGT GGATTAAGAG CACTTCCAAG GTCCCCTCCC 
TGTTCTCCTT CTCCCAACCT AGGGGAGAGC AGCTTCTCTA GTGACTGGGA TGATGAGAGT 
CTTGGTGACG ATGTGTTTGA GGTTCCACCC AGCCCATTGG CCGCTCACCG GAACTTTACA 
TACCATGGGC AGGAGCTTAT TGGCAGACAT TCCCCAGACC TGCTCTCAAG ACCATCACTT 
GGAAGCACCT CTACCCCTCG GAATTTGTCT CCTGAATTCA GCATGAGAAA GAGGTCTGCT 
TTGCCTCTAA CTCCTAATGT CAGCCGGATT AGTCAGGAGT CTTCAGGCAG TGTGAAGTCT 
ACTAGCCCCT CCACCAGTCA TAGTTCCTCT GGATTTGTCG ATGCCGAGGT CCAACGTTCT 
TCATTTCTCC CTCGCAATCT GCTCAGCATG TTTGACGATG CCACCGAGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825560.1
CDS	69..1802
Translation	MFWRQFSPVSPKSEQGPPLHSPLTCLLIMPVPGDDMGDTPLTRTPIPVPAHFSQAEQSFSLKKRGRSLCYTLPPRPPVKSALPISRLFPNKQRSWSQPRPQSVSFRSPQNNPPASELYDQSKGETFFKQCFRSICKLGRGSFGEVYKVQSLEDGCFYAVKRSVSPFRGESDRQRKLQEVRKHERVGVHPNCLRFVRAWEEKRLLYLQTELCAGSLQQHSEEFSGPLPPRKIWNITCDLLHGLKHLHDRNLLHLDIKPANVFISFSGVCKLGDFGLMVELDGTEGSGEAQEGDPRYMAPELLDGIFSKAADVFSLGMSLLEVACNMELPKSGDGWQQLRQGRLPTEFTSDLPPDFLKVLAAMLEPDYRRRATVDWLLSLPAIRNAERWRMVTLAQERTLGKIITVYQFIVWLVSIVFQGLNRPVVGFLHYCGLRALPRSPPCSPSPNLGESSFSSDWDDESLGDDVFEVPPSPLAAHRNFTYHGQELIGRHSPDLLSRPSLGSTSTPRNLSPEFSMRKRSALPLTPNVSRISQESSGSVKSTSPSTSHSSSGFVDAEVQRSSFLPRNLLSMFDDATEQ

Target ORF information:

RefSeq Version	XM_012970106.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970106.2

ORF Insert Sequence:

ATGTTTTGGC GCCAATTTTC TCCTGTATCT CCGAAGAGCG AACAAGGACC GCCCTTACAT 
TCACCTTTAA CGTGCCTGCT CATTATGCCT GTTCCAGGAG ATGACATGGG AGATACTCCC 
CTTACTCGCA CCCCTATCCC TGTGCCTGCT CACTTCAGTC AGGCAGAGCA AAGCTTTTCA 
CTTAAAAAAA GAGGGCGCTC CCTTTGCTAT ACCCTGCCAC CCAGACCTCC AGTTAAGAGC 
GCTCTGCCTA TTAGCCGCCT ATTCCCGAAC AAACAGCGGT CTTGGAGCCA GCCTAGGCCA 
CAAAGTGTTT CTTTCCGAAG TCCCCAGAAC AATCCCCCTG CAAGCGAGCT CTATGACCAG 
AGCAAAGGAG AGACCTTTTT CAAGCAGTGT TTTAGAAGCA TTTGCAAATT AGGCCGAGGA 
TCCTTTGGCG AGGTATACAA GGTGCAAAGC CTTGAGGATG GATGCTTCTA TGCCGTGAAG 
CGCTCAGTTT CTCCTTTCCG TGGCGAGTCA GACCGCCAAC GGAAGCTTCA GGAGGTGAGG 
AAGCATGAGA GAGTCGGGGT GCACCCCAAC TGCCTGCGTT TTGTGCGAGC ATGGGAAGAG 
AAACGATTGC TCTACCTGCA GACGGAGTTA TGTGCAGGGA GTCTGCAACA GCACTCGGAA 
GAATTTTCTG GGCCCCTCCC TCCACGTAAA ATATGGAACA TAACTTGTGA CCTGTTGCAC 
GGTCTCAAAC ATCTTCATGA TCGCAACCTT CTGCACCTTG ACATTAAACC AGCTAACGTC 
TTCATTTCAT TTTCTGGTGT TTGCAAACTG GGTGACTTTG GACTCATGGT AGAATTAGAT 
GGAACCGAAG GAAGTGGAGA AGCGCAGGAA GGTGACCCTC GATACATGGC ACCTGAACTC 
TTGGATGGGA TCTTTTCGAA GGCCGCTGAT GTGTTCAGCC TTGGAATGAG TCTTCTAGAG 
GTCGCTTGCA ATATGGAGCT TCCAAAAAGT GGAGACGGCT GGCAGCAGCT AAGACAAGGG 
CGTCTCCCTA CAGAGTTCAC ATCAGACCTG CCGCCAGACT TTCTGAAAGT TCTTGCTGCA 
ATGCTGGAGC CTGACTACCG CCGGCGTGCC ACAGTGGACT GGCTGCTCTC CCTTCCTGCT 
ATCCGTAATG CAGAGAGATG GAGGATGGTG ACACTAGCTC AGGAAAGGAC ACTTGGAAAG 
ATAATTACAG TCTATCAGTT CATCGTTTGG CTTGTATCTA TCGTGTTTCA AGGTCTCAAC 
CGCCCAGTTG TAGGATTTTT ACATTACTGT GGATTAAGAG CACTTCCAAG GTCCCCTCCC 
TGTTCTCCTT CTCCCAACCT AGGGGAGAGC AGCTTCTCTA GTGACTGGGA TGATGAGAGT 
CTTGGTGACG ATGTGTTTGA GGTTCCACCC AGCCCATTGG CCGCTCACCG GAACTTTACA 
TACCATGGGC AGGAGCTTAT TGGCAGACAT TCCCCAGACC TGCTCTCAAG ACCATCACTT 
GGAAGCACCT CTACCCCTCG GAATTTGTCT CCTGAATTCA GCATGAGAAA GAGGTCTGCT 
TTGCCTCTAA CTCCTAATGT CAGCCGGATT AGTCAGGAGT CTTCAGGCAG TGTGAAGTCT 
ACTAGCCCCT CCACCAGTCA TAGTTCCTCT GGATTTGTCG ATGCCGAGGT CCAACGTTCT 
TCATTTCTCC CTCGCAATCT GCTCAGCATG TTTGACGATG CCACCGAGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkmyt1 ( XM_012970107.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825561.1 Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X3, mRNA.

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CloneID	OXa01536
Clone ID Related Accession (Same CDS sequence)	XM_012970107.2 , XM_012970107.3 , NM_001127159.1
Accession Version	XM_012970107.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970107.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTTC CAGGAGATGA CATGGGAGAT ACTCCCCTTA CTCGCACCCC TATCCCTGTG 
CCTGCTCACT TCAGTCAGGC AGAGCAAAGC TTTTCACTTA AAAAAAGAGG GCGCTCCCTT 
TGCTATACCC TGCCACCCAG ACCTCCAGTT AAGAGCGCTC TGCCTATTAG CCGCCTATTC 
CCGAACAAAC AGCGGTCTTG GAGCCAGCCT AGGCCACAAA GTGTTTCTTT CCGAAGTCCC 
CAGAACAATC CCCCTGCAAG CGAGCTCTAT GACCAGAGCA AAGGAGAGAC CTTTTTCAAG 
CAGTGTTTTA GAAGCATTTG CAAATTAGGC CGAGGATCCT TTGGCGAGGT ATACAAGGTG 
CAAAGCCTTG AGGATGGATG CTTCTATGCC GTGAAGCGCT CAGTTTCTCC TTTCCGTGGC 
GAGTCAGACC GCCAACGGAA GCTTCAGGAG GTGAGGAAGC ATGAGAGAGT CGGGGTGCAC 
CCCAACTGCC TGCGTTTTGT GCGAGCATGG GAAGAGAAAC GATTGCTCTA CCTGCAGACG 
GAGTTATGTG CAGGGAGTCT GCAACAGCAC TCGGAAGAAT TTTCTGGGCC CCTCCCTCCA 
CGTAAAATAT GGAACATAAC TTGTGACCTG TTGCACGGTC TCAAACATCT TCATGATCGC 
AACCTTCTGC ACCTTGACAT TAAACCAGCT AACGTCTTCA TTTCATTTTC TGGTGTTTGC 
AAACTGGGTG ACTTTGGACT CATGGTAGAA TTAGATGGAA CCGAAGGAAG TGGAGAAGCG 
CAGGAAGGTG ACCCTCGATA CATGGCACCT GAACTCTTGG ATGGGATCTT TTCGAAGGCC 
GCTGATGTGT TCAGCCTTGG AATGAGTCTT CTAGAGGTCG CTTGCAATAT GGAGCTTCCA 
AAAAGTGGAG ACGGCTGGCA GCAGCTAAGA CAAGGGCGTC TCCCTACAGA GTTCACATCA 
GACCTGCCGC CAGACTTTCT GAAAGTTCTT GCTGCAATGC TGGAGCCTGA CTACCGCCGG 
CGTGCCACAG TGGACTGGCT GCTCTCCCTT CCTGCTATCC GTAATGCAGA GAGATGGAGG 
ATGGTGACAC TAGCTCAGGA AAGGACACTT GGAAAGATAA TTACAGTCTA TCAGTTCATC 
GTTTGGCTTG TATCTATCGT GTTTCAAGGT CTCAACCGCC CAGTTGTAGG ATTTTTACAT 
TACTGTGGAT TAAGAGCACT TCCAAGGTCC CCTCCCTGTT CTCCTTCTCC CAACCTAGGG 
GAGAGCAGCT TCTCTAGTGA CTGGGATGAT GAGAGTCTTG GTGACGATGT GTTTGAGGTT 
CCACCCAGCC CATTGGCCGC TCACCGGAAC TTTACATACC ATGGGCAGGA GCTTATTGGC 
AGACATTCCC CAGACCTGCT CTCAAGACCA TCACTTGGAA GCACCTCTAC CCCTCGGAAT 
TTGTCTCCTG AATTCAGCAT GAGAAAGAGG TCTGCTTTGC CTCTAACTCC TAATGTCAGC 
CGGATTAGTC AGGAGTCTTC AGGCAGTGTG AAGTCTACTA GCCCCTCCAC CAGTCATAGT 
TCCTCTGGAT TTGTCGATGC CGAGGTCCAA CGTTCTTCAT TTCTCCCTCG CAATCTGCTC 
AGCATGTTTG ACGATGCCAC CGAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825561.1
CDS	1197..2846
Translation	MPVPGDDMGDTPLTRTPIPVPAHFSQAEQSFSLKKRGRSLCYTLPPRPPVKSALPISRLFPNKQRSWSQPRPQSVSFRSPQNNPPASELYDQSKGETFFKQCFRSICKLGRGSFGEVYKVQSLEDGCFYAVKRSVSPFRGESDRQRKLQEVRKHERVGVHPNCLRFVRAWEEKRLLYLQTELCAGSLQQHSEEFSGPLPPRKIWNITCDLLHGLKHLHDRNLLHLDIKPANVFISFSGVCKLGDFGLMVELDGTEGSGEAQEGDPRYMAPELLDGIFSKAADVFSLGMSLLEVACNMELPKSGDGWQQLRQGRLPTEFTSDLPPDFLKVLAAMLEPDYRRRATVDWLLSLPAIRNAERWRMVTLAQERTLGKIITVYQFIVWLVSIVFQGLNRPVVGFLHYCGLRALPRSPPCSPSPNLGESSFSSDWDDESLGDDVFEVPPSPLAAHRNFTYHGQELIGRHSPDLLSRPSLGSTSTPRNLSPEFSMRKRSALPLTPNVSRISQESSGSVKSTSPSTSHSSSGFVDAEVQRSSFLPRNLLSMFDDATEQ

Target ORF information:

RefSeq Version	XM_012970107.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970107.3

ORF Insert Sequence:

ATGCCTGTTC CAGGAGATGA CATGGGAGAT ACTCCCCTTA CTCGCACCCC TATCCCTGTG 
CCTGCTCACT TCAGTCAGGC AGAGCAAAGC TTTTCACTTA AAAAAAGAGG GCGCTCCCTT 
TGCTATACCC TGCCACCCAG ACCTCCAGTT AAGAGCGCTC TGCCTATTAG CCGCCTATTC 
CCGAACAAAC AGCGGTCTTG GAGCCAGCCT AGGCCACAAA GTGTTTCTTT CCGAAGTCCC 
CAGAACAATC CCCCTGCAAG CGAGCTCTAT GACCAGAGCA AAGGAGAGAC CTTTTTCAAG 
CAGTGTTTTA GAAGCATTTG CAAATTAGGC CGAGGATCCT TTGGCGAGGT ATACAAGGTG 
CAAAGCCTTG AGGATGGATG CTTCTATGCC GTGAAGCGCT CAGTTTCTCC TTTCCGTGGC 
GAGTCAGACC GCCAACGGAA GCTTCAGGAG GTGAGGAAGC ATGAGAGAGT CGGGGTGCAC 
CCCAACTGCC TGCGTTTTGT GCGAGCATGG GAAGAGAAAC GATTGCTCTA CCTGCAGACG 
GAGTTATGTG CAGGGAGTCT GCAACAGCAC TCGGAAGAAT TTTCTGGGCC CCTCCCTCCA 
CGTAAAATAT GGAACATAAC TTGTGACCTG TTGCACGGTC TCAAACATCT TCATGATCGC 
AACCTTCTGC ACCTTGACAT TAAACCAGCT AACGTCTTCA TTTCATTTTC TGGTGTTTGC 
AAACTGGGTG ACTTTGGACT CATGGTAGAA TTAGATGGAA CCGAAGGAAG TGGAGAAGCG 
CAGGAAGGTG ACCCTCGATA CATGGCACCT GAACTCTTGG ATGGGATCTT TTCGAAGGCC 
GCTGATGTGT TCAGCCTTGG AATGAGTCTT CTAGAGGTCG CTTGCAATAT GGAGCTTCCA 
AAAAGTGGAG ACGGCTGGCA GCAGCTAAGA CAAGGGCGTC TCCCTACAGA GTTCACATCA 
GACCTGCCGC CAGACTTTCT GAAAGTTCTT GCTGCAATGC TGGAGCCTGA CTACCGCCGG 
CGTGCCACAG TGGACTGGCT GCTCTCCCTT CCTGCTATCC GTAATGCAGA GAGATGGAGG 
ATGGTGACAC TAGCTCAGGA AAGGACACTT GGAAAGATAA TTACAGTCTA TCAGTTCATC 
GTTTGGCTTG TATCTATCGT GTTTCAAGGT CTCAACCGCC CAGTTGTAGG ATTTTTACAT 
TACTGTGGAT TAAGAGCACT TCCAAGGTCC CCTCCCTGTT CTCCTTCTCC CAACCTAGGG 
GAGAGCAGCT TCTCTAGTGA CTGGGATGAT GAGAGTCTTG GTGACGATGT GTTTGAGGTT 
CCACCCAGCC CATTGGCCGC TCACCGGAAC TTTACATACC ATGGGCAGGA GCTTATTGGC 
AGACATTCCC CAGACCTGCT CTCAAGACCA TCACTTGGAA GCACCTCTAC CCCTCGGAAT 
TTGTCTCCTG AATTCAGCAT GAGAAAGAGG TCTGCTTTGC CTCTAACTCC TAATGTCAGC 
CGGATTAGTC AGGAGTCTTC AGGCAGTGTG AAGTCTACTA GCCCCTCCAC CAGTCATAGT 
TCCTCTGGAT TTGTCGATGC CGAGGTCCAA CGTTCTTCAT TTCTCCCTCG CAATCTGCTC 
AGCATGTTTG ACGATGCCAC CGAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkmyt1 ( XM_012970105.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825559.1 Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa32054
Clone ID Related Accession (Same CDS sequence)	XM_012970105.2 , XM_012970105.3
Accession Version	XM_012970105.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCGCA ACCGCGCAGC CGCGCTTGGA GGGGCGGGCC GCCATCTTGT GTGTGGCATA 
CCTGGGATAG TGGGCGGCGC TGCTTCCCCA CCTAAGTCTC CTGTATCTCC GAAGAGCGAA 
CAAGGACCGC CCTTACATTC ACCTTTAACG TGCCTGCTCA TTATGCCTGT TCCAGGAGAT 
GACATGGGAG ATACTCCCCT TACTCGCACC CCTATCCCTG TGCCTGCTCA CTTCAGTCAG 
GCAGAGCAAA GCTTTTCACT TAAAAAAAGA GGGCGCTCCC TTTGCTATAC CCTGCCACCC 
AGACCTCCAG TTAAGAGCGC TCTGCCTATT AGCCGCCTAT TCCCGAACAA ACAGCGGTCT 
TGGAGCCAGC CTAGGCCACA AAGTGTTTCT TTCCGAAGTC CCCAGAACAA TCCCCCTGCA 
AGCGAGCTCT ATGACCAGAG CAAAGGAGAG ACCTTTTTCA AGCAGTGTTT TAGAAGCATT 
TGCAAATTAG GCCGAGGATC CTTTGGCGAG GTATACAAGG TGCAAAGCCT TGAGGATGGA 
TGCTTCTATG CCGTGAAGCG CTCAGTTTCT CCTTTCCGTG GCGAGTCAGA CCGCCAACGG 
AAGCTTCAGG AGGTGAGGAA GCATGAGAGA GTCGGGGTGC ACCCCAACTG CCTGCGTTTT 
GTGCGAGCAT GGGAAGAGAA ACGATTGCTC TACCTGCAGA CGGAGTTATG TGCAGGGAGT 
CTGCAACAGC ACTCGGAAGA ATTTTCTGGG CCCCTCCCTC CACGTAAAAT ATGGAACATA 
ACTTGTGACC TGTTGCACGG TCTCAAACAT CTTCATGATC GCAACCTTCT GCACCTTGAC 
ATTAAACCAG CTAACGTCTT CATTTCATTT TCTGGTGTTT GCAAACTGGG TGACTTTGGA 
CTCATGGTAG AATTAGATGG AACCGAAGGA AGTGGAGAAG CGCAGGAAGG TGACCCTCGA 
TACATGGCAC CTGAACTCTT GGATGGGATC TTTTCGAAGG CCGCTGATGT GTTCAGCCTT 
GGAATGAGTC TTCTAGAGGT CGCTTGCAAT ATGGAGCTTC CAAAAAGTGG AGACGGCTGG 
CAGCAGCTAA GACAAGGGCG TCTCCCTACA GAGTTCACAT CAGACCTGCC GCCAGACTTT 
CTGAAAGTTC TTGCTGCAAT GCTGGAGCCT GACTACCGCC GGCGTGCCAC AGTGGACTGG 
CTGCTCTCCC TTCCTGCTAT CCGTAATGCA GAGAGATGGA GGATGGTGAC ACTAGCTCAG 
GAAAGGACAC TTGGAAAGAT AATTACAGTC TATCAGTTCA TCGTTTGGCT TGTATCTATC 
GTGTTTCAAG GTCTCAACCG CCCAGTTGTA GGATTTTTAC ATTACTGTGG ATTAAGAGCA 
CTTCCAAGGT CCCCTCCCTG TTCTCCTTCT CCCAACCTAG GGGAGAGCAG CTTCTCTAGT 
GACTGGGATG ATGAGAGTCT TGGTGACGAT GTGTTTGAGG TTCCACCCAG CCCATTGGCC 
GCTCACCGGA ACTTTACATA CCATGGGCAG GAGCTTATTG GCAGACATTC CCCAGACCTG 
CTCTCAAGAC CATCACTTGG AAGCACCTCT ACCCCTCGGA ATTTGTCTCC TGAATTCAGC 
ATGAGAAAGA GGTCTGCTTT GCCTCTAACT CCTAATGTCA GCCGGATTAG TCAGGAGTCT 
TCAGGCAGTG TGAAGTCTAC TAGCCCCTCC ACCAGTCATA GTTCCTCTGG ATTTGTCGAT 
GCCGAGGTCC AACGTTCTTC ATTTCTCCCT CGCAATCTGC TCAGCATGTT TGACGATGCC 
ACCGAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825559.1
CDS	26..1837
Translation	MHRNRAAALGGAGRHLVCGIPGIVGGAASPPKSPVSPKSEQGPPLHSPLTCLLIMPVPGDDMGDTPLTRTPIPVPAHFSQAEQSFSLKKRGRSLCYTLPPRPPVKSALPISRLFPNKQRSWSQPRPQSVSFRSPQNNPPASELYDQSKGETFFKQCFRSICKLGRGSFGEVYKVQSLEDGCFYAVKRSVSPFRGESDRQRKLQEVRKHERVGVHPNCLRFVRAWEEKRLLYLQTELCAGSLQQHSEEFSGPLPPRKIWNITCDLLHGLKHLHDRNLLHLDIKPANVFISFSGVCKLGDFGLMVELDGTEGSGEAQEGDPRYMAPELLDGIFSKAADVFSLGMSLLEVACNMELPKSGDGWQQLRQGRLPTEFTSDLPPDFLKVLAAMLEPDYRRRATVDWLLSLPAIRNAERWRMVTLAQERTLGKIITVYQFIVWLVSIVFQGLNRPVVGFLHYCGLRALPRSPPCSPSPNLGESSFSSDWDDESLGDDVFEVPPSPLAAHRNFTYHGQELIGRHSPDLLSRPSLGSTSTPRNLSPEFSMRKRSALPLTPNVSRISQESSGSVKSTSPSTSHSSSGFVDAEVQRSSFLPRNLLSMFDDATEQ

Target ORF information:

RefSeq Version	XM_012970105.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970105.3

ORF Insert Sequence:

ATGCACCGCA ACCGCGCAGC CGCGCTTGGA GGGGCGGGCC GCCATCTTGT GTGTGGCATA 
CCTGGGATAG TGGGCGGCGC TGCTTCCCCA CCTAAGTCTC CTGTATCTCC GAAGAGCGAA 
CAAGGACCGC CCTTACATTC ACCTTTAACG TGCCTGCTCA TTATGCCTGT TCCAGGAGAT 
GACATGGGAG ATACTCCCCT TACTCGCACC CCTATCCCTG TGCCTGCTCA CTTCAGTCAG 
GCAGAGCAAA GCTTTTCACT TAAAAAAAGA GGGCGCTCCC TTTGCTATAC CCTGCCACCC 
AGACCTCCAG TTAAGAGCGC TCTGCCTATT AGCCGCCTAT TCCCGAACAA ACAGCGGTCT 
TGGAGCCAGC CTAGGCCACA AAGTGTTTCT TTCCGAAGTC CCCAGAACAA TCCCCCTGCA 
AGCGAGCTCT ATGACCAGAG CAAAGGAGAG ACCTTTTTCA AGCAGTGTTT TAGAAGCATT 
TGCAAATTAG GCCGAGGATC CTTTGGCGAG GTATACAAGG TGCAAAGCCT TGAGGATGGA 
TGCTTCTATG CCGTGAAGCG CTCAGTTTCT CCTTTCCGTG GCGAGTCAGA CCGCCAACGG 
AAGCTTCAGG AGGTGAGGAA GCATGAGAGA GTCGGGGTGC ACCCCAACTG CCTGCGTTTT 
GTGCGAGCAT GGGAAGAGAA ACGATTGCTC TACCTGCAGA CGGAGTTATG TGCAGGGAGT 
CTGCAACAGC ACTCGGAAGA ATTTTCTGGG CCCCTCCCTC CACGTAAAAT ATGGAACATA 
ACTTGTGACC TGTTGCACGG TCTCAAACAT CTTCATGATC GCAACCTTCT GCACCTTGAC 
ATTAAACCAG CTAACGTCTT CATTTCATTT TCTGGTGTTT GCAAACTGGG TGACTTTGGA 
CTCATGGTAG AATTAGATGG AACCGAAGGA AGTGGAGAAG CGCAGGAAGG TGACCCTCGA 
TACATGGCAC CTGAACTCTT GGATGGGATC TTTTCGAAGG CCGCTGATGT GTTCAGCCTT 
GGAATGAGTC TTCTAGAGGT CGCTTGCAAT ATGGAGCTTC CAAAAAGTGG AGACGGCTGG 
CAGCAGCTAA GACAAGGGCG TCTCCCTACA GAGTTCACAT CAGACCTGCC GCCAGACTTT 
CTGAAAGTTC TTGCTGCAAT GCTGGAGCCT GACTACCGCC GGCGTGCCAC AGTGGACTGG 
CTGCTCTCCC TTCCTGCTAT CCGTAATGCA GAGAGATGGA GGATGGTGAC ACTAGCTCAG 
GAAAGGACAC TTGGAAAGAT AATTACAGTC TATCAGTTCA TCGTTTGGCT TGTATCTATC 
GTGTTTCAAG GTCTCAACCG CCCAGTTGTA GGATTTTTAC ATTACTGTGG ATTAAGAGCA 
CTTCCAAGGT CCCCTCCCTG TTCTCCTTCT CCCAACCTAG GGGAGAGCAG CTTCTCTAGT 
GACTGGGATG ATGAGAGTCT TGGTGACGAT GTGTTTGAGG TTCCACCCAG CCCATTGGCC 
GCTCACCGGA ACTTTACATA CCATGGGCAG GAGCTTATTG GCAGACATTC CCCAGACCTG 
CTCTCAAGAC CATCACTTGG AAGCACCTCT ACCCCTCGGA ATTTGTCTCC TGAATTCAGC 
ATGAGAAAGA GGTCTGCTTT GCCTCTAACT CCTAATGTCA GCCGGATTAG TCAGGAGTCT 
TCAGGCAGTG TGAAGTCTAC TAGCCCCTCC ACCAGTCATA GTTCCTCTGG ATTTGTCGAT 
GCCGAGGTCC AACGTTCTTC ATTTCTCCCT CGCAATCTGC TCAGCATGTT TGACGATGCC 
ACCGAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkmyt1 ( XM_012970106.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825560.1 Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X2, mRNA.

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CloneID	OXa32055
Clone ID Related Accession (Same CDS sequence)	XM_012970106.2 , XM_012970106.3
Accession Version	XM_012970106.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970106.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGGC GCCAATTTTC TCCTGTATCT CCGAAGAGCG AACAAGGACC GCCCTTACAT 
TCACCTTTAA CGTGCCTGCT CATTATGCCT GTTCCAGGAG ATGACATGGG AGATACTCCC 
CTTACTCGCA CCCCTATCCC TGTGCCTGCT CACTTCAGTC AGGCAGAGCA AAGCTTTTCA 
CTTAAAAAAA GAGGGCGCTC CCTTTGCTAT ACCCTGCCAC CCAGACCTCC AGTTAAGAGC 
GCTCTGCCTA TTAGCCGCCT ATTCCCGAAC AAACAGCGGT CTTGGAGCCA GCCTAGGCCA 
CAAAGTGTTT CTTTCCGAAG TCCCCAGAAC AATCCCCCTG CAAGCGAGCT CTATGACCAG 
AGCAAAGGAG AGACCTTTTT CAAGCAGTGT TTTAGAAGCA TTTGCAAATT AGGCCGAGGA 
TCCTTTGGCG AGGTATACAA GGTGCAAAGC CTTGAGGATG GATGCTTCTA TGCCGTGAAG 
CGCTCAGTTT CTCCTTTCCG TGGCGAGTCA GACCGCCAAC GGAAGCTTCA GGAGGTGAGG 
AAGCATGAGA GAGTCGGGGT GCACCCCAAC TGCCTGCGTT TTGTGCGAGC ATGGGAAGAG 
AAACGATTGC TCTACCTGCA GACGGAGTTA TGTGCAGGGA GTCTGCAACA GCACTCGGAA 
GAATTTTCTG GGCCCCTCCC TCCACGTAAA ATATGGAACA TAACTTGTGA CCTGTTGCAC 
GGTCTCAAAC ATCTTCATGA TCGCAACCTT CTGCACCTTG ACATTAAACC AGCTAACGTC 
TTCATTTCAT TTTCTGGTGT TTGCAAACTG GGTGACTTTG GACTCATGGT AGAATTAGAT 
GGAACCGAAG GAAGTGGAGA AGCGCAGGAA GGTGACCCTC GATACATGGC ACCTGAACTC 
TTGGATGGGA TCTTTTCGAA GGCCGCTGAT GTGTTCAGCC TTGGAATGAG TCTTCTAGAG 
GTCGCTTGCA ATATGGAGCT TCCAAAAAGT GGAGACGGCT GGCAGCAGCT AAGACAAGGG 
CGTCTCCCTA CAGAGTTCAC ATCAGACCTG CCGCCAGACT TTCTGAAAGT TCTTGCTGCA 
ATGCTGGAGC CTGACTACCG CCGGCGTGCC ACAGTGGACT GGCTGCTCTC CCTTCCTGCT 
ATCCGTAATG CAGAGAGATG GAGGATGGTG ACACTAGCTC AGGAAAGGAC ACTTGGAAAG 
ATAATTACAG TCTATCAGTT CATCGTTTGG CTTGTATCTA TCGTGTTTCA AGGTCTCAAC 
CGCCCAGTTG TAGGATTTTT ACATTACTGT GGATTAAGAG CACTTCCAAG GTCCCCTCCC 
TGTTCTCCTT CTCCCAACCT AGGGGAGAGC AGCTTCTCTA GTGACTGGGA TGATGAGAGT 
CTTGGTGACG ATGTGTTTGA GGTTCCACCC AGCCCATTGG CCGCTCACCG GAACTTTACA 
TACCATGGGC AGGAGCTTAT TGGCAGACAT TCCCCAGACC TGCTCTCAAG ACCATCACTT 
GGAAGCACCT CTACCCCTCG GAATTTGTCT CCTGAATTCA GCATGAGAAA GAGGTCTGCT 
TTGCCTCTAA CTCCTAATGT CAGCCGGATT AGTCAGGAGT CTTCAGGCAG TGTGAAGTCT 
ACTAGCCCCT CCACCAGTCA TAGTTCCTCT GGATTTGTCG ATGCCGAGGT CCAACGTTCT 
TCATTTCTCC CTCGCAATCT GCTCAGCATG TTTGACGATG CCACCGAGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825560.1
CDS	66..1799
Translation	MFWRQFSPVSPKSEQGPPLHSPLTCLLIMPVPGDDMGDTPLTRTPIPVPAHFSQAEQSFSLKKRGRSLCYTLPPRPPVKSALPISRLFPNKQRSWSQPRPQSVSFRSPQNNPPASELYDQSKGETFFKQCFRSICKLGRGSFGEVYKVQSLEDGCFYAVKRSVSPFRGESDRQRKLQEVRKHERVGVHPNCLRFVRAWEEKRLLYLQTELCAGSLQQHSEEFSGPLPPRKIWNITCDLLHGLKHLHDRNLLHLDIKPANVFISFSGVCKLGDFGLMVELDGTEGSGEAQEGDPRYMAPELLDGIFSKAADVFSLGMSLLEVACNMELPKSGDGWQQLRQGRLPTEFTSDLPPDFLKVLAAMLEPDYRRRATVDWLLSLPAIRNAERWRMVTLAQERTLGKIITVYQFIVWLVSIVFQGLNRPVVGFLHYCGLRALPRSPPCSPSPNLGESSFSSDWDDESLGDDVFEVPPSPLAAHRNFTYHGQELIGRHSPDLLSRPSLGSTSTPRNLSPEFSMRKRSALPLTPNVSRISQESSGSVKSTSPSTSHSSSGFVDAEVQRSSFLPRNLLSMFDDATEQ

Target ORF information:

RefSeq Version	XM_012970106.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein kinase, membrane associated tyrosine/threonine 1 (pkmyt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970106.3

ORF Insert Sequence:

ATGTTTTGGC GCCAATTTTC TCCTGTATCT CCGAAGAGCG AACAAGGACC GCCCTTACAT 
TCACCTTTAA CGTGCCTGCT CATTATGCCT GTTCCAGGAG ATGACATGGG AGATACTCCC 
CTTACTCGCA CCCCTATCCC TGTGCCTGCT CACTTCAGTC AGGCAGAGCA AAGCTTTTCA 
CTTAAAAAAA GAGGGCGCTC CCTTTGCTAT ACCCTGCCAC CCAGACCTCC AGTTAAGAGC 
GCTCTGCCTA TTAGCCGCCT ATTCCCGAAC AAACAGCGGT CTTGGAGCCA GCCTAGGCCA 
CAAAGTGTTT CTTTCCGAAG TCCCCAGAAC AATCCCCCTG CAAGCGAGCT CTATGACCAG 
AGCAAAGGAG AGACCTTTTT CAAGCAGTGT TTTAGAAGCA TTTGCAAATT AGGCCGAGGA 
TCCTTTGGCG AGGTATACAA GGTGCAAAGC CTTGAGGATG GATGCTTCTA TGCCGTGAAG 
CGCTCAGTTT CTCCTTTCCG TGGCGAGTCA GACCGCCAAC GGAAGCTTCA GGAGGTGAGG 
AAGCATGAGA GAGTCGGGGT GCACCCCAAC TGCCTGCGTT TTGTGCGAGC ATGGGAAGAG 
AAACGATTGC TCTACCTGCA GACGGAGTTA TGTGCAGGGA GTCTGCAACA GCACTCGGAA 
GAATTTTCTG GGCCCCTCCC TCCACGTAAA ATATGGAACA TAACTTGTGA CCTGTTGCAC 
GGTCTCAAAC ATCTTCATGA TCGCAACCTT CTGCACCTTG ACATTAAACC AGCTAACGTC 
TTCATTTCAT TTTCTGGTGT TTGCAAACTG GGTGACTTTG GACTCATGGT AGAATTAGAT 
GGAACCGAAG GAAGTGGAGA AGCGCAGGAA GGTGACCCTC GATACATGGC ACCTGAACTC 
TTGGATGGGA TCTTTTCGAA GGCCGCTGAT GTGTTCAGCC TTGGAATGAG TCTTCTAGAG 
GTCGCTTGCA ATATGGAGCT TCCAAAAAGT GGAGACGGCT GGCAGCAGCT AAGACAAGGG 
CGTCTCCCTA CAGAGTTCAC ATCAGACCTG CCGCCAGACT TTCTGAAAGT TCTTGCTGCA 
ATGCTGGAGC CTGACTACCG CCGGCGTGCC ACAGTGGACT GGCTGCTCTC CCTTCCTGCT 
ATCCGTAATG CAGAGAGATG GAGGATGGTG ACACTAGCTC AGGAAAGGAC ACTTGGAAAG 
ATAATTACAG TCTATCAGTT CATCGTTTGG CTTGTATCTA TCGTGTTTCA AGGTCTCAAC 
CGCCCAGTTG TAGGATTTTT ACATTACTGT GGATTAAGAG CACTTCCAAG GTCCCCTCCC 
TGTTCTCCTT CTCCCAACCT AGGGGAGAGC AGCTTCTCTA GTGACTGGGA TGATGAGAGT 
CTTGGTGACG ATGTGTTTGA GGTTCCACCC AGCCCATTGG CCGCTCACCG GAACTTTACA 
TACCATGGGC AGGAGCTTAT TGGCAGACAT TCCCCAGACC TGCTCTCAAG ACCATCACTT 
GGAAGCACCT CTACCCCTCG GAATTTGTCT CCTGAATTCA GCATGAGAAA GAGGTCTGCT 
TTGCCTCTAA CTCCTAATGT CAGCCGGATT AGTCAGGAGT CTTCAGGCAG TGTGAAGTCT 
ACTAGCCCCT CCACCAGTCA TAGTTCCTCT GGATTTGTCG ATGCCGAGGT CCAACGTTCT 
TCATTTCTCC CTCGCAATCT GCTCAGCATG TTTGACGATG CCACCGAGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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pkmyt1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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