pum1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following pum1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pum1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa05404 XM_002939357.5
Latest version!
Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa05405 XM_018091506.2
Latest version!
Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa05406 XM_004911559.4
Latest version!
Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa42710 XM_018091507.1
Latest version!
Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
View Old Accession Versions >>
OXa05405 XM_018091506.1 Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa05406 XM_004911559.3 Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa05404 XM_002939357.4 Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa05404
    Clone ID Related Accession (Same CDS sequence) XM_002939357.4 , XM_002939357.5
    Accession Version XM_002939357.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pumilio homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939357.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGGTATG
    TTTTCAGCAG GTTTAGCTCC AGCTGCCTTT GTTCCAAATC CCTACATAAT AAGTGCAGCG
    CCACCTGGAA CAGACCCCTA TGCAGCAGGG CTGGCAGCGG CGGCAACACT TGGTCCAGCT
    GTTGTCCCTC ACCAGTACTA TGGAGTGACG CCTTGGGGGG TGTATCCAGC CAACCTCTTT
    CAGCAGCAAG CAGCAGCTGC TGCTGCAGCA AGCAACTCTG CTTCACAGCA GAATAATCAA
    CAGAGTCAAC AGGGCCAACA GCAGGTGCTA CGTGCAGGGG GCAACCAGAG ACCATTAACA
    CCCAACCAAA ATCAGCAGGG GCAACAAACT GACCAGCTGG TGGCAGCAGC TGCAGTGAAT
    TCTGCACTTG CCTTTGGACA AGGACTAGCT GCAGGAATGC CAGGATACCC TGTGCTTGCA
    CCTGCTGCTT ACTATGATCA GACAGGTGCC CTGGTTGTGA ATACTGGAGC CAGGAATGGT
    TTGGGTGGGC CTGTTCGTCT AGTGGCACCA GCACCTGTTA TCATCAGCCC TTCAGCAGCA
    CAAGCAGCTG TTGCAGCAGC TGCTGCCTCT GCAAATGGTC CAGGTGGTAC CGCTAATGGA
    CCCTTTCGGC AGTTAAACTC TCAGCAGCCA CAGGGCCAGC CACAGCCTCA GGGCAACCAG
    AATCTAGCAT CCAGCTCATT CTATGGCAAC AGCTCTCTGA ACAGTAACTC ACAGAGCAGC
    TCATTGTTCT CACAGAGCTC TGGTCAGCCT GGGAACAGTT CTTTGGGATT TGGCAGCAGC
    GGTTCTTTAG GAGCCACACT GAGTTCAGCT CTTGGTGGAT TTGGGACTGC AGTTGCGAAT
    TCCAACACTG GCAGTGGATC TCGTCGTGAC TCTCTGACTG GCAGCAGTGA TATTTACAAA
    AGAACTTCTA GCAGTCTGAC ACCCATAGGT CACAGCTTCT ACAATGGACT GGGCTTTTCA
    TCTTCTCCAG GTCCTGTGGG AATGCCACTT CCCAGTCAGG GTCCCAATCA CTCCCAAACA
    CCACCTCCAT CCCTGTCTTC TCATGGGTCC TCCACCAGCC TCAATCTTGG AGGTTTAACA
    AATGGCAGTG GGCGATATAT TTCTGCTGCA CCAGGGGCAG AGGCAAAGTA CAGAAGTGCC
    AGCAGTGCAT CTAGCTTGTT TAGCCCAAGC AGCACTCTCT TCCCATCCTC CCGCTTGCGC
    TATGGAATGT CAGATGTCAT GCCCTCTGGA CGAAGCAGAC TACTTGAAGA TTTCAGGAAC
    AACCGCTACC CAAATTTACA GTTGCGAGAA ATTGCTGGCC ACATTATGGA GTTTTCCCAA
    GACCAGCATG GATCAAGGTT TATACAGTTA AAGCTGGAGC GAGCTACACC TGCAGAGAGA
    CAACTGGTGT TCAACGAGAT CCTTCAAGCA GCTTATCAAC TTATGGTGGA TGTATTTGGG
    AATTACGTCA TACAGAAATT TTTTGAGTTT GGCAGCCTAG AACAGAAGCT TGCTCTTGCT
    GAACGGATTC GTGGGCATGT ACTTTCACTG GCGCTACAAA TGTATGGATG TCGGGTGATA
    CAGAAGGCAC TGGAGTTTAT TCCCCCTGAT CAGCAAGTTA TTAATGAAAT GGTCAGAGAG
    TTGGATGGTC ACGTCTTGAA GTGTGTAAAG GACCAGAATG GGAATCATGT GGTTCAAAAA
    TGCATAGAGT GTGTACAGCC GCAGTCCCTT CAATTCATTA TTGATGCCTT CAAGAGCCAG
    GTATTTGCAC TCTCTACGCA TCCGTATGGC TGTCGAGTGA TCCAGCGAAT TTTGGAGCAC
    TGCCTTCCGG AGCAGACACT GCCCATTTTA GAAGAGTTAC ACCAGCACAC GGAACAGCTT
    GTTCAGGATC AGTATGGTAA CTACGTTATC CAGCATGTTT TGGAGCATGG TCGACCGGAG
    GACAAAAGTA AAATTGTTGC AGAGATTCGT GGAAATGTTC TAGTCCTGAG TCAGCACAAA
    TTTGCCAGTA ATGTAGTTGA AAAATGTGTG ACACATGCGT CTCGTACGGA AAGAGCCATG
    CTTATAGACG AGGTGTGCAC AATGAATGAT GGGCCTCATA GTGCCTTATA CACCATGATG
    AAGGACCAGT ACGCTAACTA CGTTGTACAG AAAATGATCG ATGTAGCAGA ACCAGCTCAG
    CGCAAGATTG TTATGCACAA GATCCGACCA CATATTGCCA CTCTCCGTAA ATACACGTAT
    GGCAAGCACA TCCTGGCCAA GTTGGAGAAG TACTACATGA AAAACGGCAT GGATCTGGGT
    CCAATGTGTG GACCCTCAAA TGGAATCATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939403.2
    CDS207..3779
    Translation

    Target ORF information:

    RefSeq Version XM_002939357.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939357.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGGTATG
    TTTTCAGCAG GTTTAGCTCC AGCTGCCTTT GTTCCAAATC CCTACATAAT AAGTGCAGCG
    CCACCTGGAA CAGACCCCTA TGCAGCAGGG CTGGCAGCGG CGGCAACACT TGGTCCAGCT
    GTTGTCCCTC ACCAGTACTA TGGAGTGACG CCTTGGGGGG TGTATCCAGC CAACCTCTTT
    CAGCAGCAAG CAGCAGCTGC TGCTGCAGCA AGCAACTCTG CTTCACAGCA GAATAATCAA
    CAGAGTCAAC AGGGCCAACA GCAGGTGCTA CGTGCAGGGG GCAACCAGAG ACCATTAACA
    CCCAACCAAA ATCAGCAGGG GCAACAAACT GACCAGCTGG TGGCAGCAGC TGCAGTGAAT
    TCTGCACTTG CCTTTGGACA AGGACTAGCT GCAGGAATGC CAGGATACCC TGTGCTTGCA
    CCTGCTGCTT ACTATGATCA GACAGGTGCC CTGGTTGTGA ATACTGGAGC CAGGAATGGT
    TTGGGTGGGC CTGTTCGTCT AGTGGCACCA GCACCTGTTA TCATCAGCCC TTCAGCAGCA
    CAAGCAGCTG TTGCAGCAGC TGCTGCCTCT GCAAATGGTC CAGGTGGTAC CGCTAATGGA
    CCCTTTCGGC AGTTAAACTC TCAGCAGCCA CAGGGCCAGC CACAGCCTCA GGGCAACCAG
    AATCTAGCAT CCAGCTCATT CTATGGCAAC AGCTCTCTGA ACAGTAACTC ACAGAGCAGC
    TCATTGTTCT CACAGAGCTC TGGTCAGCCT GGGAACAGTT CTTTGGGATT TGGCAGCAGC
    GGTTCTTTAG GAGCCACACT GAGTTCAGCT CTTGGTGGAT TTGGGACTGC AGTTGCGAAT
    TCCAACACTG GCAGTGGATC TCGTCGTGAC TCTCTGACTG GCAGCAGTGA TATTTACAAA
    AGAACTTCTA GCAGTCTGAC ACCCATAGGT CACAGCTTCT ACAATGGACT GGGCTTTTCA
    TCTTCTCCAG GTCCTGTGGG AATGCCACTT CCCAGTCAGG GTCCCAATCA CTCCCAAACA
    CCACCTCCAT CCCTGTCTTC TCATGGGTCC TCCACCAGCC TCAATCTTGG AGGTTTAACA
    AATGGCAGTG GGCGATATAT TTCTGCTGCA CCAGGGGCAG AGGCAAAGTA CAGAAGTGCC
    AGCAGTGCAT CTAGCTTGTT TAGCCCAAGC AGCACTCTCT TCCCATCCTC CCGCTTGCGC
    TATGGAATGT CAGATGTCAT GCCCTCTGGA CGAAGCAGAC TACTTGAAGA TTTCAGGAAC
    AACCGCTACC CAAATTTACA GTTGCGAGAA ATTGCTGGCC ACATTATGGA GTTTTCCCAA
    GACCAGCATG GATCAAGGTT TATACAGTTA AAGCTGGAGC GAGCTACACC TGCAGAGAGA
    CAACTGGTGT TCAACGAGAT CCTTCAAGCA GCTTATCAAC TTATGGTGGA TGTATTTGGG
    AATTACGTCA TACAGAAATT TTTTGAGTTT GGCAGCCTAG AACAGAAGCT TGCTCTTGCT
    GAACGGATTC GTGGGCATGT ACTTTCACTG GCGCTACAAA TGTATGGATG TCGGGTGATA
    CAGAAGGCAC TGGAGTTTAT TCCCCCTGAT CAGCAAGTTA TTAATGAAAT GGTCAGAGAG
    TTGGATGGTC ACGTCTTGAA GTGTGTAAAG GACCAGAATG GGAATCATGT GGTTCAAAAA
    TGCATAGAGT GTGTACAGCC GCAGTCCCTT CAATTCATTA TTGATGCCTT CAAGAGCCAG
    GTATTTGCAC TCTCTACGCA TCCGTATGGC TGTCGAGTGA TCCAGCGAAT TTTGGAGCAC
    TGCCTTCCGG AGCAGACACT GCCCATTTTA GAAGAGTTAC ACCAGCACAC GGAACAGCTT
    GTTCAGGATC AGTATGGTAA CTACGTTATC CAGCATGTTT TGGAGCATGG TCGACCGGAG
    GACAAAAGTA AAATTGTTGC AGAGATTCGT GGAAATGTTC TAGTCCTGAG TCAGCACAAA
    TTTGCCAGTA ATGTAGTTGA AAAATGTGTG ACACATGCGT CTCGTACGGA AAGAGCCATG
    CTTATAGACG AGGTGTGCAC AATGAATGAT GGGCCTCATA GTGCCTTATA CACCATGATG
    AAGGACCAGT ACGCTAACTA CGTTGTACAG AAAATGATCG ATGTAGCAGA ACCAGCTCAG
    CGCAAGATTG TTATGCACAA GATCCGACCA CATATTGCCA CTCTCCGTAA ATACACGTAT
    GGCAAGCACA TCCTGGCCAA GTTGGAGAAG TACTACATGA AAAACGGCAT GGATCTGGGT
    CCAATGTGTG GACCCTCAAA TGGAATCATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05405
    Clone ID Related Accession (Same CDS sequence) XM_018091506.2 , XM_018091506.1
    Accession Version XM_018091506.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pumilio homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGCAGGT
    TTAGCTCCAG CTGCCTTTGT TCCAAATCCC TACATAATAA GTGCAGCGCC ACCTGGAACA
    GACCCCTATG CAGCAGGGCT GGCAGCGGCG GCAACACTTG GTCCAGCTGT TGTCCCTCAC
    CAGTACTATG GAGTGACGCC TTGGGGGGTG TATCCAGCCA ACCTCTTTCA GCAGCAAGCA
    GCAGCTGCTG CTGCAGCAAG CAACTCTGCT TCACAGCAGA ATAATCAACA GAGTCAACAG
    GGCCAACAGC AGGTGCTACG TGCAGGGGGC AACCAGAGAC CATTAACACC CAACCAAAAT
    CAGCAGGGGC AACAAACTGA CCAGCTGGTG GCAGCAGCTG CAGTGAATTC TGCACTTGCC
    TTTGGACAAG GACTAGCTGC AGGAATGCCA GGATACCCTG TGCTTGCACC TGCTGCTTAC
    TATGATCAGA CAGGTGCCCT GGTTGTGAAT ACTGGAGCCA GGAATGGTTT GGGTGGGCCT
    GTTCGTCTAG TGGCACCAGC ACCTGTTATC ATCAGCCCTT CAGCAGCACA AGCAGCTGTT
    GCAGCAGCTG CTGCCTCTGC AAATGGTCCA GGTGGTACCG CTAATGGACC CTTTCGGCAG
    TTAAACTCTC AGCAGCCACA GGGCCAGCCA CAGCCTCAGG GCAACCAGAA TCTAGCATCC
    AGCTCATTCT ATGGCAACAG CTCTCTGAAC AGTAACTCAC AGAGCAGCTC ATTGTTCTCA
    CAGAGCTCTG GTCAGCCTGG GAACAGTTCT TTGGGATTTG GCAGCAGCGG TTCTTTAGGA
    GCCACACTGA GTTCAGCTCT TGGTGGATTT GGGACTGCAG TTGCGAATTC CAACACTGGC
    AGTGGATCTC GTCGTGACTC TCTGACTGGC AGCAGTGATA TTTACAAAAG AACTTCTAGC
    AGTCTGACAC CCATAGGTCA CAGCTTCTAC AATGGACTGG GCTTTTCATC TTCTCCAGGT
    CCTGTGGGAA TGCCACTTCC CAGTCAGGGT CCCAATCACT CCCAAACACC ACCTCCATCC
    CTGTCTTCTC ATGGGTCCTC CACCAGCCTC AATCTTGGAG GTTTAACAAA TGGCAGTGGG
    CGATATATTT CTGCTGCACC AGGGGCAGAG GCAAAGTACA GAAGTGCCAG CAGTGCATCT
    AGCTTGTTTA GCCCAAGCAG CACTCTCTTC CCATCCTCCC GCTTGCGCTA TGGAATGTCA
    GATGTCATGC CCTCTGGACG AAGCAGACTA CTTGAAGATT TCAGGAACAA CCGCTACCCA
    AATTTACAGT TGCGAGAAAT TGCTGGCCAC ATTATGGAGT TTTCCCAAGA CCAGCATGGA
    TCAAGGTTTA TACAGTTAAA GCTGGAGCGA GCTACACCTG CAGAGAGACA ACTGGTGTTC
    AACGAGATCC TTCAAGCAGC TTATCAACTT ATGGTGGATG TATTTGGGAA TTACGTCATA
    CAGAAATTTT TTGAGTTTGG CAGCCTAGAA CAGAAGCTTG CTCTTGCTGA ACGGATTCGT
    GGGCATGTAC TTTCACTGGC GCTACAAATG TATGGATGTC GGGTGATACA GAAGGCACTG
    GAGTTTATTC CCCCTGATCA GCAAGTTATT AATGAAATGG TCAGAGAGTT GGATGGTCAC
    GTCTTGAAGT GTGTAAAGGA CCAGAATGGG AATCATGTGG TTCAAAAATG CATAGAGTGT
    GTACAGCCGC AGTCCCTTCA ATTCATTATT GATGCCTTCA AGAGCCAGGT ATTTGCACTC
    TCTACGCATC CGTATGGCTG TCGAGTGATC CAGCGAATTT TGGAGCACTG CCTTCCGGAG
    CAGACACTGC CCATTTTAGA AGAGTTACAC CAGCACACGG AACAGCTTGT TCAGGATCAG
    TATGGTAACT ACGTTATCCA GCATGTTTTG GAGCATGGTC GACCGGAGGA CAAAAGTAAA
    ATTGTTGCAG AGATTCGTGG AAATGTTCTA GTCCTGAGTC AGCACAAATT TGCCAGTAAT
    GTAGTTGAAA AATGTGTGAC ACATGCGTCT CGTACGGAAA GAGCCATGCT TATAGACGAG
    GTGTGCACAA TGAATGATGG GCCTCATAGT GCCTTATACA CCATGATGAA GGACCAGTAC
    GCTAACTACG TTGTACAGAA AATGATCGAT GTAGCAGAAC CAGCTCAGCG CAAGATTGTT
    ATGCACAAGA TCCGACCACA TATTGCCACT CTCCGTAAAT ACACGTATGG CAAGCACATC
    CTGGCCAAGT TGGAGAAGTA CTACATGAAA AACGGCATGG ATCTGGGTCC AATGTGTGGA
    CCCTCAAATG GAATCATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946995.1
    CDS207..3767
    Translation

    Target ORF information:

    RefSeq Version XM_018091506.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091506.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGCAGGT
    TTAGCTCCAG CTGCCTTTGT TCCAAATCCC TACATAATAA GTGCAGCGCC ACCTGGAACA
    GACCCCTATG CAGCAGGGCT GGCAGCGGCG GCAACACTTG GTCCAGCTGT TGTCCCTCAC
    CAGTACTATG GAGTGACGCC TTGGGGGGTG TATCCAGCCA ACCTCTTTCA GCAGCAAGCA
    GCAGCTGCTG CTGCAGCAAG CAACTCTGCT TCACAGCAGA ATAATCAACA GAGTCAACAG
    GGCCAACAGC AGGTGCTACG TGCAGGGGGC AACCAGAGAC CATTAACACC CAACCAAAAT
    CAGCAGGGGC AACAAACTGA CCAGCTGGTG GCAGCAGCTG CAGTGAATTC TGCACTTGCC
    TTTGGACAAG GACTAGCTGC AGGAATGCCA GGATACCCTG TGCTTGCACC TGCTGCTTAC
    TATGATCAGA CAGGTGCCCT GGTTGTGAAT ACTGGAGCCA GGAATGGTTT GGGTGGGCCT
    GTTCGTCTAG TGGCACCAGC ACCTGTTATC ATCAGCCCTT CAGCAGCACA AGCAGCTGTT
    GCAGCAGCTG CTGCCTCTGC AAATGGTCCA GGTGGTACCG CTAATGGACC CTTTCGGCAG
    TTAAACTCTC AGCAGCCACA GGGCCAGCCA CAGCCTCAGG GCAACCAGAA TCTAGCATCC
    AGCTCATTCT ATGGCAACAG CTCTCTGAAC AGTAACTCAC AGAGCAGCTC ATTGTTCTCA
    CAGAGCTCTG GTCAGCCTGG GAACAGTTCT TTGGGATTTG GCAGCAGCGG TTCTTTAGGA
    GCCACACTGA GTTCAGCTCT TGGTGGATTT GGGACTGCAG TTGCGAATTC CAACACTGGC
    AGTGGATCTC GTCGTGACTC TCTGACTGGC AGCAGTGATA TTTACAAAAG AACTTCTAGC
    AGTCTGACAC CCATAGGTCA CAGCTTCTAC AATGGACTGG GCTTTTCATC TTCTCCAGGT
    CCTGTGGGAA TGCCACTTCC CAGTCAGGGT CCCAATCACT CCCAAACACC ACCTCCATCC
    CTGTCTTCTC ATGGGTCCTC CACCAGCCTC AATCTTGGAG GTTTAACAAA TGGCAGTGGG
    CGATATATTT CTGCTGCACC AGGGGCAGAG GCAAAGTACA GAAGTGCCAG CAGTGCATCT
    AGCTTGTTTA GCCCAAGCAG CACTCTCTTC CCATCCTCCC GCTTGCGCTA TGGAATGTCA
    GATGTCATGC CCTCTGGACG AAGCAGACTA CTTGAAGATT TCAGGAACAA CCGCTACCCA
    AATTTACAGT TGCGAGAAAT TGCTGGCCAC ATTATGGAGT TTTCCCAAGA CCAGCATGGA
    TCAAGGTTTA TACAGTTAAA GCTGGAGCGA GCTACACCTG CAGAGAGACA ACTGGTGTTC
    AACGAGATCC TTCAAGCAGC TTATCAACTT ATGGTGGATG TATTTGGGAA TTACGTCATA
    CAGAAATTTT TTGAGTTTGG CAGCCTAGAA CAGAAGCTTG CTCTTGCTGA ACGGATTCGT
    GGGCATGTAC TTTCACTGGC GCTACAAATG TATGGATGTC GGGTGATACA GAAGGCACTG
    GAGTTTATTC CCCCTGATCA GCAAGTTATT AATGAAATGG TCAGAGAGTT GGATGGTCAC
    GTCTTGAAGT GTGTAAAGGA CCAGAATGGG AATCATGTGG TTCAAAAATG CATAGAGTGT
    GTACAGCCGC AGTCCCTTCA ATTCATTATT GATGCCTTCA AGAGCCAGGT ATTTGCACTC
    TCTACGCATC CGTATGGCTG TCGAGTGATC CAGCGAATTT TGGAGCACTG CCTTCCGGAG
    CAGACACTGC CCATTTTAGA AGAGTTACAC CAGCACACGG AACAGCTTGT TCAGGATCAG
    TATGGTAACT ACGTTATCCA GCATGTTTTG GAGCATGGTC GACCGGAGGA CAAAAGTAAA
    ATTGTTGCAG AGATTCGTGG AAATGTTCTA GTCCTGAGTC AGCACAAATT TGCCAGTAAT
    GTAGTTGAAA AATGTGTGAC ACATGCGTCT CGTACGGAAA GAGCCATGCT TATAGACGAG
    GTGTGCACAA TGAATGATGG GCCTCATAGT GCCTTATACA CCATGATGAA GGACCAGTAC
    GCTAACTACG TTGTACAGAA AATGATCGAT GTAGCAGAAC CAGCTCAGCG CAAGATTGTT
    ATGCACAAGA TCCGACCACA TATTGCCACT CTCCGTAAAT ACACGTATGG CAAGCACATC
    CTGGCCAAGT TGGAGAAGTA CTACATGAAA AACGGCATGG ATCTGGGTCC AATGTGTGGA
    CCCTCAAATG GAATCATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05406
    Clone ID Related Accession (Same CDS sequence) XM_004911559.4 , XM_004911559.3
    Accession Version XM_004911559.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pumilio homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911559.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGGTTTA
    GCTCCAGCTG CCTTTGTTCC AAATCCCTAC ATAATAAGTG CAGCGCCACC TGGAACAGAC
    CCCTATGCAG CAGGGCTGGC AGCGGCGGCA ACACTTGGTC CAGCTGTTGT CCCTCACCAG
    TACTATGGAG TGACGCCTTG GGGGGTGTAT CCAGCCAACC TCTTTCAGCA GCAAGCAGCA
    GCTGCTGCTG CAGCAAGCAA CTCTGCTTCA CAGCAGAATA ATCAACAGAG TCAACAGGGC
    CAACAGCAGG TGCTACGTGC AGGGGGCAAC CAGAGACCAT TAACACCCAA CCAAAATCAG
    CAGGGGCAAC AAACTGACCA GCTGGTGGCA GCAGCTGCAG TGAATTCTGC ACTTGCCTTT
    GGACAAGGAC TAGCTGCAGG AATGCCAGGA TACCCTGTGC TTGCACCTGC TGCTTACTAT
    GATCAGACAG GTGCCCTGGT TGTGAATACT GGAGCCAGGA ATGGTTTGGG TGGGCCTGTT
    CGTCTAGTGG CACCAGCACC TGTTATCATC AGCCCTTCAG CAGCACAAGC AGCTGTTGCA
    GCAGCTGCTG CCTCTGCAAA TGGTCCAGGT GGTACCGCTA ATGGACCCTT TCGGCAGTTA
    AACTCTCAGC AGCCACAGGG CCAGCCACAG CCTCAGGGCA ACCAGAATCT AGCATCCAGC
    TCATTCTATG GCAACAGCTC TCTGAACAGT AACTCACAGA GCAGCTCATT GTTCTCACAG
    AGCTCTGGTC AGCCTGGGAA CAGTTCTTTG GGATTTGGCA GCAGCGGTTC TTTAGGAGCC
    ACACTGAGTT CAGCTCTTGG TGGATTTGGG ACTGCAGTTG CGAATTCCAA CACTGGCAGT
    GGATCTCGTC GTGACTCTCT GACTGGCAGC AGTGATATTT ACAAAAGAAC TTCTAGCAGT
    CTGACACCCA TAGGTCACAG CTTCTACAAT GGACTGGGCT TTTCATCTTC TCCAGGTCCT
    GTGGGAATGC CACTTCCCAG TCAGGGTCCC AATCACTCCC AAACACCACC TCCATCCCTG
    TCTTCTCATG GGTCCTCCAC CAGCCTCAAT CTTGGAGGTT TAACAAATGG CAGTGGGCGA
    TATATTTCTG CTGCACCAGG GGCAGAGGCA AAGTACAGAA GTGCCAGCAG TGCATCTAGC
    TTGTTTAGCC CAAGCAGCAC TCTCTTCCCA TCCTCCCGCT TGCGCTATGG AATGTCAGAT
    GTCATGCCCT CTGGACGAAG CAGACTACTT GAAGATTTCA GGAACAACCG CTACCCAAAT
    TTACAGTTGC GAGAAATTGC TGGCCACATT ATGGAGTTTT CCCAAGACCA GCATGGATCA
    AGGTTTATAC AGTTAAAGCT GGAGCGAGCT ACACCTGCAG AGAGACAACT GGTGTTCAAC
    GAGATCCTTC AAGCAGCTTA TCAACTTATG GTGGATGTAT TTGGGAATTA CGTCATACAG
    AAATTTTTTG AGTTTGGCAG CCTAGAACAG AAGCTTGCTC TTGCTGAACG GATTCGTGGG
    CATGTACTTT CACTGGCGCT ACAAATGTAT GGATGTCGGG TGATACAGAA GGCACTGGAG
    TTTATTCCCC CTGATCAGCA AGTTATTAAT GAAATGGTCA GAGAGTTGGA TGGTCACGTC
    TTGAAGTGTG TAAAGGACCA GAATGGGAAT CATGTGGTTC AAAAATGCAT AGAGTGTGTA
    CAGCCGCAGT CCCTTCAATT CATTATTGAT GCCTTCAAGA GCCAGGTATT TGCACTCTCT
    ACGCATCCGT ATGGCTGTCG AGTGATCCAG CGAATTTTGG AGCACTGCCT TCCGGAGCAG
    ACACTGCCCA TTTTAGAAGA GTTACACCAG CACACGGAAC AGCTTGTTCA GGATCAGTAT
    GGTAACTACG TTATCCAGCA TGTTTTGGAG CATGGTCGAC CGGAGGACAA AAGTAAAATT
    GTTGCAGAGA TTCGTGGAAA TGTTCTAGTC CTGAGTCAGC ACAAATTTGC CAGTAATGTA
    GTTGAAAAAT GTGTGACACA TGCGTCTCGT ACGGAAAGAG CCATGCTTAT AGACGAGGTG
    TGCACAATGA ATGATGGGCC TCATAGTGCC TTATACACCA TGATGAAGGA CCAGTACGCT
    AACTACGTTG TACAGAAAAT GATCGATGTA GCAGAACCAG CTCAGCGCAA GATTGTTATG
    CACAAGATCC GACCACATAT TGCCACTCTC CGTAAATACA CGTATGGCAA GCACATCCTG
    GCCAAGTTGG AGAAGTACTA CATGAAAAAC GGCATGGATC TGGGTCCAAT GTGTGGACCC
    TCAAATGGAA TCATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911616.1
    CDS206..3763
    Translation

    Target ORF information:

    RefSeq Version XM_004911559.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911559.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGGTTTA
    GCTCCAGCTG CCTTTGTTCC AAATCCCTAC ATAATAAGTG CAGCGCCACC TGGAACAGAC
    CCCTATGCAG CAGGGCTGGC AGCGGCGGCA ACACTTGGTC CAGCTGTTGT CCCTCACCAG
    TACTATGGAG TGACGCCTTG GGGGGTGTAT CCAGCCAACC TCTTTCAGCA GCAAGCAGCA
    GCTGCTGCTG CAGCAAGCAA CTCTGCTTCA CAGCAGAATA ATCAACAGAG TCAACAGGGC
    CAACAGCAGG TGCTACGTGC AGGGGGCAAC CAGAGACCAT TAACACCCAA CCAAAATCAG
    CAGGGGCAAC AAACTGACCA GCTGGTGGCA GCAGCTGCAG TGAATTCTGC ACTTGCCTTT
    GGACAAGGAC TAGCTGCAGG AATGCCAGGA TACCCTGTGC TTGCACCTGC TGCTTACTAT
    GATCAGACAG GTGCCCTGGT TGTGAATACT GGAGCCAGGA ATGGTTTGGG TGGGCCTGTT
    CGTCTAGTGG CACCAGCACC TGTTATCATC AGCCCTTCAG CAGCACAAGC AGCTGTTGCA
    GCAGCTGCTG CCTCTGCAAA TGGTCCAGGT GGTACCGCTA ATGGACCCTT TCGGCAGTTA
    AACTCTCAGC AGCCACAGGG CCAGCCACAG CCTCAGGGCA ACCAGAATCT AGCATCCAGC
    TCATTCTATG GCAACAGCTC TCTGAACAGT AACTCACAGA GCAGCTCATT GTTCTCACAG
    AGCTCTGGTC AGCCTGGGAA CAGTTCTTTG GGATTTGGCA GCAGCGGTTC TTTAGGAGCC
    ACACTGAGTT CAGCTCTTGG TGGATTTGGG ACTGCAGTTG CGAATTCCAA CACTGGCAGT
    GGATCTCGTC GTGACTCTCT GACTGGCAGC AGTGATATTT ACAAAAGAAC TTCTAGCAGT
    CTGACACCCA TAGGTCACAG CTTCTACAAT GGACTGGGCT TTTCATCTTC TCCAGGTCCT
    GTGGGAATGC CACTTCCCAG TCAGGGTCCC AATCACTCCC AAACACCACC TCCATCCCTG
    TCTTCTCATG GGTCCTCCAC CAGCCTCAAT CTTGGAGGTT TAACAAATGG CAGTGGGCGA
    TATATTTCTG CTGCACCAGG GGCAGAGGCA AAGTACAGAA GTGCCAGCAG TGCATCTAGC
    TTGTTTAGCC CAAGCAGCAC TCTCTTCCCA TCCTCCCGCT TGCGCTATGG AATGTCAGAT
    GTCATGCCCT CTGGACGAAG CAGACTACTT GAAGATTTCA GGAACAACCG CTACCCAAAT
    TTACAGTTGC GAGAAATTGC TGGCCACATT ATGGAGTTTT CCCAAGACCA GCATGGATCA
    AGGTTTATAC AGTTAAAGCT GGAGCGAGCT ACACCTGCAG AGAGACAACT GGTGTTCAAC
    GAGATCCTTC AAGCAGCTTA TCAACTTATG GTGGATGTAT TTGGGAATTA CGTCATACAG
    AAATTTTTTG AGTTTGGCAG CCTAGAACAG AAGCTTGCTC TTGCTGAACG GATTCGTGGG
    CATGTACTTT CACTGGCGCT ACAAATGTAT GGATGTCGGG TGATACAGAA GGCACTGGAG
    TTTATTCCCC CTGATCAGCA AGTTATTAAT GAAATGGTCA GAGAGTTGGA TGGTCACGTC
    TTGAAGTGTG TAAAGGACCA GAATGGGAAT CATGTGGTTC AAAAATGCAT AGAGTGTGTA
    CAGCCGCAGT CCCTTCAATT CATTATTGAT GCCTTCAAGA GCCAGGTATT TGCACTCTCT
    ACGCATCCGT ATGGCTGTCG AGTGATCCAG CGAATTTTGG AGCACTGCCT TCCGGAGCAG
    ACACTGCCCA TTTTAGAAGA GTTACACCAG CACACGGAAC AGCTTGTTCA GGATCAGTAT
    GGTAACTACG TTATCCAGCA TGTTTTGGAG CATGGTCGAC CGGAGGACAA AAGTAAAATT
    GTTGCAGAGA TTCGTGGAAA TGTTCTAGTC CTGAGTCAGC ACAAATTTGC CAGTAATGTA
    GTTGAAAAAT GTGTGACACA TGCGTCTCGT ACGGAAAGAG CCATGCTTAT AGACGAGGTG
    TGCACAATGA ATGATGGGCC TCATAGTGCC TTATACACCA TGATGAAGGA CCAGTACGCT
    AACTACGTTG TACAGAAAAT GATCGATGTA GCAGAACCAG CTCAGCGCAA GATTGTTATG
    CACAAGATCC GACCACATAT TGCCACTCTC CGTAAATACA CGTATGGCAA GCACATCCTG
    GCCAAGTTGG AGAAGTACTA CATGAAAAAC GGCATGGATC TGGGTCCAAT GTGTGGACCC
    TCAAATGGAA TCATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42710
    Clone ID Related Accession (Same CDS sequence) XM_018091507.1
    Accession Version XM_018091507.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pumilio homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGGTATG
    TTTTCAGCAG GTTTAGCTCC AGCTGCCTTT GTTCCAAATC CCTACATAAT AAGTGCAGCG
    CCACCTGGAA CAGACCCCTA TGCAGCAGGG CTGGCAGCGG CGGCAACACT TGGTCCAGCT
    GTTGTCCCTC ACCAGTACTA TGGAGTGACG CCTTGGGGGG TGTATCCAGC CAACCTCTTT
    CAGCAGCAAG CAGCAGCTGC TGCTGCAGCA AGCAACTCTG CTTCACAGCA GAATAATCAA
    CAGAGTCAAC AGGGCCAACA GCAGGTGCTA CGTGCAGGGG GCAACCAGAG ACCATTAACA
    CCCAACCAAA ATCAGCAGGG GCAACAAACT GACCAGCTGG TGGCAGCAGC TGCAGTGAAT
    TCTGCACTTG CCTTTGGACA AGGACTAGCT GCAGGAATGC CAGGATACCC TGTGCTTGCA
    CCTGCTGCTT ACTATGATCA GACAGGTGCC CTGGTTGTGA ATACTGGAGC CAGGAATGGT
    TTGGGTGGGC CTGTTCGTCT AGTGGCACCA GCACCTGTTA TCATCAGCCC TTCAGCAGCA
    CAAGCAGTTG CGAATTCCAA CACTGGCAGT GGATCTCGTC GTGACTCTCT GACTGGCAGC
    AGTGATATTT ACAAAAGAAC TTCTAGCAGT CTGACACCCA TAGGTCACAG CTTCTACAAT
    GGACTGGGCT TTTCATCTTC TCCAGGTCCT GTGGGAATGC CACTTCCCAG TCAGGGTCCC
    AATCACTCCC AAACACCACC TCCATCCCTG TCTTCTCATG GGTCCTCCAC CAGCCTCAAT
    CTTGGAGGTT TAACAAATGG CAGTGGGCGA TATATTTCTG CTGCACCAGG GGCAGAGGCA
    AAGTACAGAA GTGCCAGCAG TGCATCTAGC TTGTTTAGCC CAAGCAGCAC TCTCTTCCCA
    TCCTCCCGCT TGCGCTATGG AATGTCAGAT GTCATGCCCT CTGGACGAAG CAGACTACTT
    GAAGATTTCA GGAACAACCG CTACCCAAAT TTACAGTTGC GAGAAATTGC TGGCCACATT
    ATGGAGTTTT CCCAAGACCA GCATGGATCA AGGTTTATAC AGTTAAAGCT GGAGCGAGCT
    ACACCTGCAG AGAGACAACT GGTGTTCAAC GAGATCCTTC AAGCAGCTTA TCAACTTATG
    GTGGATGTAT TTGGGAATTA CGTCATACAG AAATTTTTTG AGTTTGGCAG CCTAGAACAG
    AAGCTTGCTC TTGCTGAACG GATTCGTGGG CATGTACTTT CACTGGCGCT ACAAATGTAT
    GGATGTCGGG TGATACAGAA GGCACTGGAG TTTATTCCCC CTGATCAGCA AGTTATTAAT
    GAAATGGTCA GAGAGTTGGA TGGTCACGTC TTGAAGTGTG TAAAGGACCA GAATGGGAAT
    CATGTGGTTC AAAAATGCAT AGAGTGTGTA CAGCCGCAGT CCCTTCAATT CATTATTGAT
    GCCTTCAAGA GCCAGGTATT TGCACTCTCT ACGCATCCGT ATGGCTGTCG AGTGATCCAG
    CGAATTTTGG AGCACTGCCT TCCGGAGCAG ACACTGCCCA TTTTAGAAGA GTTACACCAG
    CACACGGAAC AGCTTGTTCA GGATCAGTAT GGTAACTACG TTATCCAGCA TGTTTTGGAG
    CATGGTCGAC CGGAGGACAA AAGTAAAATT GTTGCAGAGA TTCGTGGAAA TGTTCTAGTC
    CTGAGTCAGC ACAAATTTGC CAGTAATGTA GTTGAAAAAT GTGTGACACA TGCGTCTCGT
    ACGGAAAGAG CCATGCTTAT AGACGAGGTG TGCACAATGA ATGATGGGCC TCATAGTGCC
    TTATACACCA TGATGAAGGA CCAGTACGCT AACTACGTTG TACAGAAAAT GATCGATGTA
    GCAGAACCAG CTCAGCGCAA GATTGTTATG CACAAGATCC GACCACATAT TGCCACTCTC
    CGTAAATACA CGTATGGCAA GCACATCCTG GCCAAGTTGG AGAAGTACTA CATGAAAAAC
    GGCATGGATC TGGGTCCAAT GTGTGGACCC TCAAATGGAA TCATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946996.1
    CDS12..3299
    Translation

    Target ORF information:

    RefSeq Version XM_018091507.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091507.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGGTATG
    TTTTCAGCAG GTTTAGCTCC AGCTGCCTTT GTTCCAAATC CCTACATAAT AAGTGCAGCG
    CCACCTGGAA CAGACCCCTA TGCAGCAGGG CTGGCAGCGG CGGCAACACT TGGTCCAGCT
    GTTGTCCCTC ACCAGTACTA TGGAGTGACG CCTTGGGGGG TGTATCCAGC CAACCTCTTT
    CAGCAGCAAG CAGCAGCTGC TGCTGCAGCA AGCAACTCTG CTTCACAGCA GAATAATCAA
    CAGAGTCAAC AGGGCCAACA GCAGGTGCTA CGTGCAGGGG GCAACCAGAG ACCATTAACA
    CCCAACCAAA ATCAGCAGGG GCAACAAACT GACCAGCTGG TGGCAGCAGC TGCAGTGAAT
    TCTGCACTTG CCTTTGGACA AGGACTAGCT GCAGGAATGC CAGGATACCC TGTGCTTGCA
    CCTGCTGCTT ACTATGATCA GACAGGTGCC CTGGTTGTGA ATACTGGAGC CAGGAATGGT
    TTGGGTGGGC CTGTTCGTCT AGTGGCACCA GCACCTGTTA TCATCAGCCC TTCAGCAGCA
    CAAGCAGTTG CGAATTCCAA CACTGGCAGT GGATCTCGTC GTGACTCTCT GACTGGCAGC
    AGTGATATTT ACAAAAGAAC TTCTAGCAGT CTGACACCCA TAGGTCACAG CTTCTACAAT
    GGACTGGGCT TTTCATCTTC TCCAGGTCCT GTGGGAATGC CACTTCCCAG TCAGGGTCCC
    AATCACTCCC AAACACCACC TCCATCCCTG TCTTCTCATG GGTCCTCCAC CAGCCTCAAT
    CTTGGAGGTT TAACAAATGG CAGTGGGCGA TATATTTCTG CTGCACCAGG GGCAGAGGCA
    AAGTACAGAA GTGCCAGCAG TGCATCTAGC TTGTTTAGCC CAAGCAGCAC TCTCTTCCCA
    TCCTCCCGCT TGCGCTATGG AATGTCAGAT GTCATGCCCT CTGGACGAAG CAGACTACTT
    GAAGATTTCA GGAACAACCG CTACCCAAAT TTACAGTTGC GAGAAATTGC TGGCCACATT
    ATGGAGTTTT CCCAAGACCA GCATGGATCA AGGTTTATAC AGTTAAAGCT GGAGCGAGCT
    ACACCTGCAG AGAGACAACT GGTGTTCAAC GAGATCCTTC AAGCAGCTTA TCAACTTATG
    GTGGATGTAT TTGGGAATTA CGTCATACAG AAATTTTTTG AGTTTGGCAG CCTAGAACAG
    AAGCTTGCTC TTGCTGAACG GATTCGTGGG CATGTACTTT CACTGGCGCT ACAAATGTAT
    GGATGTCGGG TGATACAGAA GGCACTGGAG TTTATTCCCC CTGATCAGCA AGTTATTAAT
    GAAATGGTCA GAGAGTTGGA TGGTCACGTC TTGAAGTGTG TAAAGGACCA GAATGGGAAT
    CATGTGGTTC AAAAATGCAT AGAGTGTGTA CAGCCGCAGT CCCTTCAATT CATTATTGAT
    GCCTTCAAGA GCCAGGTATT TGCACTCTCT ACGCATCCGT ATGGCTGTCG AGTGATCCAG
    CGAATTTTGG AGCACTGCCT TCCGGAGCAG ACACTGCCCA TTTTAGAAGA GTTACACCAG
    CACACGGAAC AGCTTGTTCA GGATCAGTAT GGTAACTACG TTATCCAGCA TGTTTTGGAG
    CATGGTCGAC CGGAGGACAA AAGTAAAATT GTTGCAGAGA TTCGTGGAAA TGTTCTAGTC
    CTGAGTCAGC ACAAATTTGC CAGTAATGTA GTTGAAAAAT GTGTGACACA TGCGTCTCGT
    ACGGAAAGAG CCATGCTTAT AGACGAGGTG TGCACAATGA ATGATGGGCC TCATAGTGCC
    TTATACACCA TGATGAAGGA CCAGTACGCT AACTACGTTG TACAGAAAAT GATCGATGTA
    GCAGAACCAG CTCAGCGCAA GATTGTTATG CACAAGATCC GACCACATAT TGCCACTCTC
    CGTAAATACA CGTATGGCAA GCACATCCTG GCCAAGTTGG AGAAGTACTA CATGAAAAAC
    GGCATGGATC TGGGTCCAAT GTGTGGACCC TCAAATGGAA TCATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05404
    Clone ID Related Accession (Same CDS sequence) XM_002939357.4 , XM_002939357.5
    Accession Version XM_002939357.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pumilio homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939357.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGGTATG
    TTTTCAGCAG GTTTAGCTCC AGCTGCCTTT GTTCCAAATC CCTACATAAT AAGTGCAGCG
    CCACCTGGAA CAGACCCCTA TGCAGCAGGG CTGGCAGCGG CGGCAACACT TGGTCCAGCT
    GTTGTCCCTC ACCAGTACTA TGGAGTGACG CCTTGGGGGG TGTATCCAGC CAACCTCTTT
    CAGCAGCAAG CAGCAGCTGC TGCTGCAGCA AGCAACTCTG CTTCACAGCA GAATAATCAA
    CAGAGTCAAC AGGGCCAACA GCAGGTGCTA CGTGCAGGGG GCAACCAGAG ACCATTAACA
    CCCAACCAAA ATCAGCAGGG GCAACAAACT GACCAGCTGG TGGCAGCAGC TGCAGTGAAT
    TCTGCACTTG CCTTTGGACA AGGACTAGCT GCAGGAATGC CAGGATACCC TGTGCTTGCA
    CCTGCTGCTT ACTATGATCA GACAGGTGCC CTGGTTGTGA ATACTGGAGC CAGGAATGGT
    TTGGGTGGGC CTGTTCGTCT AGTGGCACCA GCACCTGTTA TCATCAGCCC TTCAGCAGCA
    CAAGCAGCTG TTGCAGCAGC TGCTGCCTCT GCAAATGGTC CAGGTGGTAC CGCTAATGGA
    CCCTTTCGGC AGTTAAACTC TCAGCAGCCA CAGGGCCAGC CACAGCCTCA GGGCAACCAG
    AATCTAGCAT CCAGCTCATT CTATGGCAAC AGCTCTCTGA ACAGTAACTC ACAGAGCAGC
    TCATTGTTCT CACAGAGCTC TGGTCAGCCT GGGAACAGTT CTTTGGGATT TGGCAGCAGC
    GGTTCTTTAG GAGCCACACT GAGTTCAGCT CTTGGTGGAT TTGGGACTGC AGTTGCGAAT
    TCCAACACTG GCAGTGGATC TCGTCGTGAC TCTCTGACTG GCAGCAGTGA TATTTACAAA
    AGAACTTCTA GCAGTCTGAC ACCCATAGGT CACAGCTTCT ACAATGGACT GGGCTTTTCA
    TCTTCTCCAG GTCCTGTGGG AATGCCACTT CCCAGTCAGG GTCCCAATCA CTCCCAAACA
    CCACCTCCAT CCCTGTCTTC TCATGGGTCC TCCACCAGCC TCAATCTTGG AGGTTTAACA
    AATGGCAGTG GGCGATATAT TTCTGCTGCA CCAGGGGCAG AGGCAAAGTA CAGAAGTGCC
    AGCAGTGCAT CTAGCTTGTT TAGCCCAAGC AGCACTCTCT TCCCATCCTC CCGCTTGCGC
    TATGGAATGT CAGATGTCAT GCCCTCTGGA CGAAGCAGAC TACTTGAAGA TTTCAGGAAC
    AACCGCTACC CAAATTTACA GTTGCGAGAA ATTGCTGGCC ACATTATGGA GTTTTCCCAA
    GACCAGCATG GATCAAGGTT TATACAGTTA AAGCTGGAGC GAGCTACACC TGCAGAGAGA
    CAACTGGTGT TCAACGAGAT CCTTCAAGCA GCTTATCAAC TTATGGTGGA TGTATTTGGG
    AATTACGTCA TACAGAAATT TTTTGAGTTT GGCAGCCTAG AACAGAAGCT TGCTCTTGCT
    GAACGGATTC GTGGGCATGT ACTTTCACTG GCGCTACAAA TGTATGGATG TCGGGTGATA
    CAGAAGGCAC TGGAGTTTAT TCCCCCTGAT CAGCAAGTTA TTAATGAAAT GGTCAGAGAG
    TTGGATGGTC ACGTCTTGAA GTGTGTAAAG GACCAGAATG GGAATCATGT GGTTCAAAAA
    TGCATAGAGT GTGTACAGCC GCAGTCCCTT CAATTCATTA TTGATGCCTT CAAGAGCCAG
    GTATTTGCAC TCTCTACGCA TCCGTATGGC TGTCGAGTGA TCCAGCGAAT TTTGGAGCAC
    TGCCTTCCGG AGCAGACACT GCCCATTTTA GAAGAGTTAC ACCAGCACAC GGAACAGCTT
    GTTCAGGATC AGTATGGTAA CTACGTTATC CAGCATGTTT TGGAGCATGG TCGACCGGAG
    GACAAAAGTA AAATTGTTGC AGAGATTCGT GGAAATGTTC TAGTCCTGAG TCAGCACAAA
    TTTGCCAGTA ATGTAGTTGA AAAATGTGTG ACACATGCGT CTCGTACGGA AAGAGCCATG
    CTTATAGACG AGGTGTGCAC AATGAATGAT GGGCCTCATA GTGCCTTATA CACCATGATG
    AAGGACCAGT ACGCTAACTA CGTTGTACAG AAAATGATCG ATGTAGCAGA ACCAGCTCAG
    CGCAAGATTG TTATGCACAA GATCCGACCA CATATTGCCA CTCTCCGTAA ATACACGTAT
    GGCAAGCACA TCCTGGCCAA GTTGGAGAAG TACTACATGA AAAACGGCAT GGATCTGGGT
    CCAATGTGTG GACCCTCAAA TGGAATCATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939403.2
    CDS212..3784
    Translation

    Target ORF information:

    RefSeq Version XM_002939357.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939357.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGGTATG
    TTTTCAGCAG GTTTAGCTCC AGCTGCCTTT GTTCCAAATC CCTACATAAT AAGTGCAGCG
    CCACCTGGAA CAGACCCCTA TGCAGCAGGG CTGGCAGCGG CGGCAACACT TGGTCCAGCT
    GTTGTCCCTC ACCAGTACTA TGGAGTGACG CCTTGGGGGG TGTATCCAGC CAACCTCTTT
    CAGCAGCAAG CAGCAGCTGC TGCTGCAGCA AGCAACTCTG CTTCACAGCA GAATAATCAA
    CAGAGTCAAC AGGGCCAACA GCAGGTGCTA CGTGCAGGGG GCAACCAGAG ACCATTAACA
    CCCAACCAAA ATCAGCAGGG GCAACAAACT GACCAGCTGG TGGCAGCAGC TGCAGTGAAT
    TCTGCACTTG CCTTTGGACA AGGACTAGCT GCAGGAATGC CAGGATACCC TGTGCTTGCA
    CCTGCTGCTT ACTATGATCA GACAGGTGCC CTGGTTGTGA ATACTGGAGC CAGGAATGGT
    TTGGGTGGGC CTGTTCGTCT AGTGGCACCA GCACCTGTTA TCATCAGCCC TTCAGCAGCA
    CAAGCAGCTG TTGCAGCAGC TGCTGCCTCT GCAAATGGTC CAGGTGGTAC CGCTAATGGA
    CCCTTTCGGC AGTTAAACTC TCAGCAGCCA CAGGGCCAGC CACAGCCTCA GGGCAACCAG
    AATCTAGCAT CCAGCTCATT CTATGGCAAC AGCTCTCTGA ACAGTAACTC ACAGAGCAGC
    TCATTGTTCT CACAGAGCTC TGGTCAGCCT GGGAACAGTT CTTTGGGATT TGGCAGCAGC
    GGTTCTTTAG GAGCCACACT GAGTTCAGCT CTTGGTGGAT TTGGGACTGC AGTTGCGAAT
    TCCAACACTG GCAGTGGATC TCGTCGTGAC TCTCTGACTG GCAGCAGTGA TATTTACAAA
    AGAACTTCTA GCAGTCTGAC ACCCATAGGT CACAGCTTCT ACAATGGACT GGGCTTTTCA
    TCTTCTCCAG GTCCTGTGGG AATGCCACTT CCCAGTCAGG GTCCCAATCA CTCCCAAACA
    CCACCTCCAT CCCTGTCTTC TCATGGGTCC TCCACCAGCC TCAATCTTGG AGGTTTAACA
    AATGGCAGTG GGCGATATAT TTCTGCTGCA CCAGGGGCAG AGGCAAAGTA CAGAAGTGCC
    AGCAGTGCAT CTAGCTTGTT TAGCCCAAGC AGCACTCTCT TCCCATCCTC CCGCTTGCGC
    TATGGAATGT CAGATGTCAT GCCCTCTGGA CGAAGCAGAC TACTTGAAGA TTTCAGGAAC
    AACCGCTACC CAAATTTACA GTTGCGAGAA ATTGCTGGCC ACATTATGGA GTTTTCCCAA
    GACCAGCATG GATCAAGGTT TATACAGTTA AAGCTGGAGC GAGCTACACC TGCAGAGAGA
    CAACTGGTGT TCAACGAGAT CCTTCAAGCA GCTTATCAAC TTATGGTGGA TGTATTTGGG
    AATTACGTCA TACAGAAATT TTTTGAGTTT GGCAGCCTAG AACAGAAGCT TGCTCTTGCT
    GAACGGATTC GTGGGCATGT ACTTTCACTG GCGCTACAAA TGTATGGATG TCGGGTGATA
    CAGAAGGCAC TGGAGTTTAT TCCCCCTGAT CAGCAAGTTA TTAATGAAAT GGTCAGAGAG
    TTGGATGGTC ACGTCTTGAA GTGTGTAAAG GACCAGAATG GGAATCATGT GGTTCAAAAA
    TGCATAGAGT GTGTACAGCC GCAGTCCCTT CAATTCATTA TTGATGCCTT CAAGAGCCAG
    GTATTTGCAC TCTCTACGCA TCCGTATGGC TGTCGAGTGA TCCAGCGAAT TTTGGAGCAC
    TGCCTTCCGG AGCAGACACT GCCCATTTTA GAAGAGTTAC ACCAGCACAC GGAACAGCTT
    GTTCAGGATC AGTATGGTAA CTACGTTATC CAGCATGTTT TGGAGCATGG TCGACCGGAG
    GACAAAAGTA AAATTGTTGC AGAGATTCGT GGAAATGTTC TAGTCCTGAG TCAGCACAAA
    TTTGCCAGTA ATGTAGTTGA AAAATGTGTG ACACATGCGT CTCGTACGGA AAGAGCCATG
    CTTATAGACG AGGTGTGCAC AATGAATGAT GGGCCTCATA GTGCCTTATA CACCATGATG
    AAGGACCAGT ACGCTAACTA CGTTGTACAG AAAATGATCG ATGTAGCAGA ACCAGCTCAG
    CGCAAGATTG TTATGCACAA GATCCGACCA CATATTGCCA CTCTCCGTAA ATACACGTAT
    GGCAAGCACA TCCTGGCCAA GTTGGAGAAG TACTACATGA AAAACGGCAT GGATCTGGGT
    CCAATGTGTG GACCCTCAAA TGGAATCATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05405
    Clone ID Related Accession (Same CDS sequence) XM_018091506.2 , XM_018091506.1
    Accession Version XM_018091506.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pumilio homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGCAGGT
    TTAGCTCCAG CTGCCTTTGT TCCAAATCCC TACATAATAA GTGCAGCGCC ACCTGGAACA
    GACCCCTATG CAGCAGGGCT GGCAGCGGCG GCAACACTTG GTCCAGCTGT TGTCCCTCAC
    CAGTACTATG GAGTGACGCC TTGGGGGGTG TATCCAGCCA ACCTCTTTCA GCAGCAAGCA
    GCAGCTGCTG CTGCAGCAAG CAACTCTGCT TCACAGCAGA ATAATCAACA GAGTCAACAG
    GGCCAACAGC AGGTGCTACG TGCAGGGGGC AACCAGAGAC CATTAACACC CAACCAAAAT
    CAGCAGGGGC AACAAACTGA CCAGCTGGTG GCAGCAGCTG CAGTGAATTC TGCACTTGCC
    TTTGGACAAG GACTAGCTGC AGGAATGCCA GGATACCCTG TGCTTGCACC TGCTGCTTAC
    TATGATCAGA CAGGTGCCCT GGTTGTGAAT ACTGGAGCCA GGAATGGTTT GGGTGGGCCT
    GTTCGTCTAG TGGCACCAGC ACCTGTTATC ATCAGCCCTT CAGCAGCACA AGCAGCTGTT
    GCAGCAGCTG CTGCCTCTGC AAATGGTCCA GGTGGTACCG CTAATGGACC CTTTCGGCAG
    TTAAACTCTC AGCAGCCACA GGGCCAGCCA CAGCCTCAGG GCAACCAGAA TCTAGCATCC
    AGCTCATTCT ATGGCAACAG CTCTCTGAAC AGTAACTCAC AGAGCAGCTC ATTGTTCTCA
    CAGAGCTCTG GTCAGCCTGG GAACAGTTCT TTGGGATTTG GCAGCAGCGG TTCTTTAGGA
    GCCACACTGA GTTCAGCTCT TGGTGGATTT GGGACTGCAG TTGCGAATTC CAACACTGGC
    AGTGGATCTC GTCGTGACTC TCTGACTGGC AGCAGTGATA TTTACAAAAG AACTTCTAGC
    AGTCTGACAC CCATAGGTCA CAGCTTCTAC AATGGACTGG GCTTTTCATC TTCTCCAGGT
    CCTGTGGGAA TGCCACTTCC CAGTCAGGGT CCCAATCACT CCCAAACACC ACCTCCATCC
    CTGTCTTCTC ATGGGTCCTC CACCAGCCTC AATCTTGGAG GTTTAACAAA TGGCAGTGGG
    CGATATATTT CTGCTGCACC AGGGGCAGAG GCAAAGTACA GAAGTGCCAG CAGTGCATCT
    AGCTTGTTTA GCCCAAGCAG CACTCTCTTC CCATCCTCCC GCTTGCGCTA TGGAATGTCA
    GATGTCATGC CCTCTGGACG AAGCAGACTA CTTGAAGATT TCAGGAACAA CCGCTACCCA
    AATTTACAGT TGCGAGAAAT TGCTGGCCAC ATTATGGAGT TTTCCCAAGA CCAGCATGGA
    TCAAGGTTTA TACAGTTAAA GCTGGAGCGA GCTACACCTG CAGAGAGACA ACTGGTGTTC
    AACGAGATCC TTCAAGCAGC TTATCAACTT ATGGTGGATG TATTTGGGAA TTACGTCATA
    CAGAAATTTT TTGAGTTTGG CAGCCTAGAA CAGAAGCTTG CTCTTGCTGA ACGGATTCGT
    GGGCATGTAC TTTCACTGGC GCTACAAATG TATGGATGTC GGGTGATACA GAAGGCACTG
    GAGTTTATTC CCCCTGATCA GCAAGTTATT AATGAAATGG TCAGAGAGTT GGATGGTCAC
    GTCTTGAAGT GTGTAAAGGA CCAGAATGGG AATCATGTGG TTCAAAAATG CATAGAGTGT
    GTACAGCCGC AGTCCCTTCA ATTCATTATT GATGCCTTCA AGAGCCAGGT ATTTGCACTC
    TCTACGCATC CGTATGGCTG TCGAGTGATC CAGCGAATTT TGGAGCACTG CCTTCCGGAG
    CAGACACTGC CCATTTTAGA AGAGTTACAC CAGCACACGG AACAGCTTGT TCAGGATCAG
    TATGGTAACT ACGTTATCCA GCATGTTTTG GAGCATGGTC GACCGGAGGA CAAAAGTAAA
    ATTGTTGCAG AGATTCGTGG AAATGTTCTA GTCCTGAGTC AGCACAAATT TGCCAGTAAT
    GTAGTTGAAA AATGTGTGAC ACATGCGTCT CGTACGGAAA GAGCCATGCT TATAGACGAG
    GTGTGCACAA TGAATGATGG GCCTCATAGT GCCTTATACA CCATGATGAA GGACCAGTAC
    GCTAACTACG TTGTACAGAA AATGATCGAT GTAGCAGAAC CAGCTCAGCG CAAGATTGTT
    ATGCACAAGA TCCGACCACA TATTGCCACT CTCCGTAAAT ACACGTATGG CAAGCACATC
    CTGGCCAAGT TGGAGAAGTA CTACATGAAA AACGGCATGG ATCTGGGTCC AATGTGTGGA
    CCCTCAAATG GAATCATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946995.1
    CDS12..3572
    Translation

    Target ORF information:

    RefSeq Version XM_018091506.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091506.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGCAGGT
    TTAGCTCCAG CTGCCTTTGT TCCAAATCCC TACATAATAA GTGCAGCGCC ACCTGGAACA
    GACCCCTATG CAGCAGGGCT GGCAGCGGCG GCAACACTTG GTCCAGCTGT TGTCCCTCAC
    CAGTACTATG GAGTGACGCC TTGGGGGGTG TATCCAGCCA ACCTCTTTCA GCAGCAAGCA
    GCAGCTGCTG CTGCAGCAAG CAACTCTGCT TCACAGCAGA ATAATCAACA GAGTCAACAG
    GGCCAACAGC AGGTGCTACG TGCAGGGGGC AACCAGAGAC CATTAACACC CAACCAAAAT
    CAGCAGGGGC AACAAACTGA CCAGCTGGTG GCAGCAGCTG CAGTGAATTC TGCACTTGCC
    TTTGGACAAG GACTAGCTGC AGGAATGCCA GGATACCCTG TGCTTGCACC TGCTGCTTAC
    TATGATCAGA CAGGTGCCCT GGTTGTGAAT ACTGGAGCCA GGAATGGTTT GGGTGGGCCT
    GTTCGTCTAG TGGCACCAGC ACCTGTTATC ATCAGCCCTT CAGCAGCACA AGCAGCTGTT
    GCAGCAGCTG CTGCCTCTGC AAATGGTCCA GGTGGTACCG CTAATGGACC CTTTCGGCAG
    TTAAACTCTC AGCAGCCACA GGGCCAGCCA CAGCCTCAGG GCAACCAGAA TCTAGCATCC
    AGCTCATTCT ATGGCAACAG CTCTCTGAAC AGTAACTCAC AGAGCAGCTC ATTGTTCTCA
    CAGAGCTCTG GTCAGCCTGG GAACAGTTCT TTGGGATTTG GCAGCAGCGG TTCTTTAGGA
    GCCACACTGA GTTCAGCTCT TGGTGGATTT GGGACTGCAG TTGCGAATTC CAACACTGGC
    AGTGGATCTC GTCGTGACTC TCTGACTGGC AGCAGTGATA TTTACAAAAG AACTTCTAGC
    AGTCTGACAC CCATAGGTCA CAGCTTCTAC AATGGACTGG GCTTTTCATC TTCTCCAGGT
    CCTGTGGGAA TGCCACTTCC CAGTCAGGGT CCCAATCACT CCCAAACACC ACCTCCATCC
    CTGTCTTCTC ATGGGTCCTC CACCAGCCTC AATCTTGGAG GTTTAACAAA TGGCAGTGGG
    CGATATATTT CTGCTGCACC AGGGGCAGAG GCAAAGTACA GAAGTGCCAG CAGTGCATCT
    AGCTTGTTTA GCCCAAGCAG CACTCTCTTC CCATCCTCCC GCTTGCGCTA TGGAATGTCA
    GATGTCATGC CCTCTGGACG AAGCAGACTA CTTGAAGATT TCAGGAACAA CCGCTACCCA
    AATTTACAGT TGCGAGAAAT TGCTGGCCAC ATTATGGAGT TTTCCCAAGA CCAGCATGGA
    TCAAGGTTTA TACAGTTAAA GCTGGAGCGA GCTACACCTG CAGAGAGACA ACTGGTGTTC
    AACGAGATCC TTCAAGCAGC TTATCAACTT ATGGTGGATG TATTTGGGAA TTACGTCATA
    CAGAAATTTT TTGAGTTTGG CAGCCTAGAA CAGAAGCTTG CTCTTGCTGA ACGGATTCGT
    GGGCATGTAC TTTCACTGGC GCTACAAATG TATGGATGTC GGGTGATACA GAAGGCACTG
    GAGTTTATTC CCCCTGATCA GCAAGTTATT AATGAAATGG TCAGAGAGTT GGATGGTCAC
    GTCTTGAAGT GTGTAAAGGA CCAGAATGGG AATCATGTGG TTCAAAAATG CATAGAGTGT
    GTACAGCCGC AGTCCCTTCA ATTCATTATT GATGCCTTCA AGAGCCAGGT ATTTGCACTC
    TCTACGCATC CGTATGGCTG TCGAGTGATC CAGCGAATTT TGGAGCACTG CCTTCCGGAG
    CAGACACTGC CCATTTTAGA AGAGTTACAC CAGCACACGG AACAGCTTGT TCAGGATCAG
    TATGGTAACT ACGTTATCCA GCATGTTTTG GAGCATGGTC GACCGGAGGA CAAAAGTAAA
    ATTGTTGCAG AGATTCGTGG AAATGTTCTA GTCCTGAGTC AGCACAAATT TGCCAGTAAT
    GTAGTTGAAA AATGTGTGAC ACATGCGTCT CGTACGGAAA GAGCCATGCT TATAGACGAG
    GTGTGCACAA TGAATGATGG GCCTCATAGT GCCTTATACA CCATGATGAA GGACCAGTAC
    GCTAACTACG TTGTACAGAA AATGATCGAT GTAGCAGAAC CAGCTCAGCG CAAGATTGTT
    ATGCACAAGA TCCGACCACA TATTGCCACT CTCCGTAAAT ACACGTATGG CAAGCACATC
    CTGGCCAAGT TGGAGAAGTA CTACATGAAA AACGGCATGG ATCTGGGTCC AATGTGTGGA
    CCCTCAAATG GAATCATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05406
    Clone ID Related Accession (Same CDS sequence) XM_004911559.4 , XM_004911559.3
    Accession Version XM_004911559.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pumilio homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911559.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGGTTTA
    GCTCCAGCTG CCTTTGTTCC AAATCCCTAC ATAATAAGTG CAGCGCCACC TGGAACAGAC
    CCCTATGCAG CAGGGCTGGC AGCGGCGGCA ACACTTGGTC CAGCTGTTGT CCCTCACCAG
    TACTATGGAG TGACGCCTTG GGGGGTGTAT CCAGCCAACC TCTTTCAGCA GCAAGCAGCA
    GCTGCTGCTG CAGCAAGCAA CTCTGCTTCA CAGCAGAATA ATCAACAGAG TCAACAGGGC
    CAACAGCAGG TGCTACGTGC AGGGGGCAAC CAGAGACCAT TAACACCCAA CCAAAATCAG
    CAGGGGCAAC AAACTGACCA GCTGGTGGCA GCAGCTGCAG TGAATTCTGC ACTTGCCTTT
    GGACAAGGAC TAGCTGCAGG AATGCCAGGA TACCCTGTGC TTGCACCTGC TGCTTACTAT
    GATCAGACAG GTGCCCTGGT TGTGAATACT GGAGCCAGGA ATGGTTTGGG TGGGCCTGTT
    CGTCTAGTGG CACCAGCACC TGTTATCATC AGCCCTTCAG CAGCACAAGC AGCTGTTGCA
    GCAGCTGCTG CCTCTGCAAA TGGTCCAGGT GGTACCGCTA ATGGACCCTT TCGGCAGTTA
    AACTCTCAGC AGCCACAGGG CCAGCCACAG CCTCAGGGCA ACCAGAATCT AGCATCCAGC
    TCATTCTATG GCAACAGCTC TCTGAACAGT AACTCACAGA GCAGCTCATT GTTCTCACAG
    AGCTCTGGTC AGCCTGGGAA CAGTTCTTTG GGATTTGGCA GCAGCGGTTC TTTAGGAGCC
    ACACTGAGTT CAGCTCTTGG TGGATTTGGG ACTGCAGTTG CGAATTCCAA CACTGGCAGT
    GGATCTCGTC GTGACTCTCT GACTGGCAGC AGTGATATTT ACAAAAGAAC TTCTAGCAGT
    CTGACACCCA TAGGTCACAG CTTCTACAAT GGACTGGGCT TTTCATCTTC TCCAGGTCCT
    GTGGGAATGC CACTTCCCAG TCAGGGTCCC AATCACTCCC AAACACCACC TCCATCCCTG
    TCTTCTCATG GGTCCTCCAC CAGCCTCAAT CTTGGAGGTT TAACAAATGG CAGTGGGCGA
    TATATTTCTG CTGCACCAGG GGCAGAGGCA AAGTACAGAA GTGCCAGCAG TGCATCTAGC
    TTGTTTAGCC CAAGCAGCAC TCTCTTCCCA TCCTCCCGCT TGCGCTATGG AATGTCAGAT
    GTCATGCCCT CTGGACGAAG CAGACTACTT GAAGATTTCA GGAACAACCG CTACCCAAAT
    TTACAGTTGC GAGAAATTGC TGGCCACATT ATGGAGTTTT CCCAAGACCA GCATGGATCA
    AGGTTTATAC AGTTAAAGCT GGAGCGAGCT ACACCTGCAG AGAGACAACT GGTGTTCAAC
    GAGATCCTTC AAGCAGCTTA TCAACTTATG GTGGATGTAT TTGGGAATTA CGTCATACAG
    AAATTTTTTG AGTTTGGCAG CCTAGAACAG AAGCTTGCTC TTGCTGAACG GATTCGTGGG
    CATGTACTTT CACTGGCGCT ACAAATGTAT GGATGTCGGG TGATACAGAA GGCACTGGAG
    TTTATTCCCC CTGATCAGCA AGTTATTAAT GAAATGGTCA GAGAGTTGGA TGGTCACGTC
    TTGAAGTGTG TAAAGGACCA GAATGGGAAT CATGTGGTTC AAAAATGCAT AGAGTGTGTA
    CAGCCGCAGT CCCTTCAATT CATTATTGAT GCCTTCAAGA GCCAGGTATT TGCACTCTCT
    ACGCATCCGT ATGGCTGTCG AGTGATCCAG CGAATTTTGG AGCACTGCCT TCCGGAGCAG
    ACACTGCCCA TTTTAGAAGA GTTACACCAG CACACGGAAC AGCTTGTTCA GGATCAGTAT
    GGTAACTACG TTATCCAGCA TGTTTTGGAG CATGGTCGAC CGGAGGACAA AAGTAAAATT
    GTTGCAGAGA TTCGTGGAAA TGTTCTAGTC CTGAGTCAGC ACAAATTTGC CAGTAATGTA
    GTTGAAAAAT GTGTGACACA TGCGTCTCGT ACGGAAAGAG CCATGCTTAT AGACGAGGTG
    TGCACAATGA ATGATGGGCC TCATAGTGCC TTATACACCA TGATGAAGGA CCAGTACGCT
    AACTACGTTG TACAGAAAAT GATCGATGTA GCAGAACCAG CTCAGCGCAA GATTGTTATG
    CACAAGATCC GACCACATAT TGCCACTCTC CGTAAATACA CGTATGGCAA GCACATCCTG
    GCCAAGTTGG AGAAGTACTA CATGAAAAAC GGCATGGATC TGGGTCCAAT GTGTGGACCC
    TCAAATGGAA TCATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911616.1
    CDS212..3769
    Translation

    Target ORF information:

    RefSeq Version XM_004911559.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pumilio RNA binding family member 1 (pum1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911559.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
    CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACCTCT
    GGACCGGTGG GCCAGCAGCA GCCACTGCAG CCCCCCACTC ATTCTGCCTT GGCAACAGGA
    CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGTTCACA GGATGACGCC
    ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TTGGAGGTGG CGGGAGTGGT
    GGAGGAGGTT ACAATAATAG CAAGCATCGC TGGCCCACTG GTGATAACAT CCACGCAGAG
    CATCAGGTTC GTTCAATGGA CGAGCTAAAT CATGATTTTC AAGCACTTGC ATTGGAAGGA
    AGAGCAATGG GAGAACAACT GTTGACGGGC AAAAAATTTT GGGAACCTGA TGATTCAAAC
    AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GAGAGAGCAC ATGGGGGGCT
    TCAGATCATT CAGTTTCCCA ACCAATTATG GTGCAGCGGA GGCCTGGCCA AGGCTTTCAT
    GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGTGTC
    AGCATGGTAG AATATGTGCT TAGCTCCTCG CCTGGAGACT CATGCCTGCG GAAAGGAGCC
    TTTGGTCCAC GAGACACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAACAAAGGT
    GTCTATGAGG GGGACAAATT AGGAGATCTA AAAGAGGAAG TAGACAGCAT GGATAAATCC
    AATGGCTTAC CAGTCCAAAA TGGGATTGAT GGAGATGTAA AGGATTTCAG CCGTACACCA
    GGAAACTGTC AAGCCTCATC AAATGAAGTA GACCTCCTTG GGCCCAGCCA GAATGGCTCA
    GAAGTGCTTG CCCAATTGAC TAATAACAAT AGTGCCAAAC CAGTAGAGGA TTTTGGTGGC
    ATTGAGTCTC AGAGTGTTCA GATAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG
    TTTGACTACC AGGGCAACCA AGTACAAGTA GATTCAGGAG CTGCCACTGT GGGCCTATTT
    GACTACAATT CACAGCAACA GCTATTCCAA AGACCAAATG CTCTGGCAGT TCAGCAGCTG
    ACAGCTGCAC AACAACAACA GTATGCACTC GCAGCAGCGC ATCAGCCACA CATAGGTTTA
    GCTCCAGCTG CCTTTGTTCC AAATCCCTAC ATAATAAGTG CAGCGCCACC TGGAACAGAC
    CCCTATGCAG CAGGGCTGGC AGCGGCGGCA ACACTTGGTC CAGCTGTTGT CCCTCACCAG
    TACTATGGAG TGACGCCTTG GGGGGTGTAT CCAGCCAACC TCTTTCAGCA GCAAGCAGCA
    GCTGCTGCTG CAGCAAGCAA CTCTGCTTCA CAGCAGAATA ATCAACAGAG TCAACAGGGC
    CAACAGCAGG TGCTACGTGC AGGGGGCAAC CAGAGACCAT TAACACCCAA CCAAAATCAG
    CAGGGGCAAC AAACTGACCA GCTGGTGGCA GCAGCTGCAG TGAATTCTGC ACTTGCCTTT
    GGACAAGGAC TAGCTGCAGG AATGCCAGGA TACCCTGTGC TTGCACCTGC TGCTTACTAT
    GATCAGACAG GTGCCCTGGT TGTGAATACT GGAGCCAGGA ATGGTTTGGG TGGGCCTGTT
    CGTCTAGTGG CACCAGCACC TGTTATCATC AGCCCTTCAG CAGCACAAGC AGCTGTTGCA
    GCAGCTGCTG CCTCTGCAAA TGGTCCAGGT GGTACCGCTA ATGGACCCTT TCGGCAGTTA
    AACTCTCAGC AGCCACAGGG CCAGCCACAG CCTCAGGGCA ACCAGAATCT AGCATCCAGC
    TCATTCTATG GCAACAGCTC TCTGAACAGT AACTCACAGA GCAGCTCATT GTTCTCACAG
    AGCTCTGGTC AGCCTGGGAA CAGTTCTTTG GGATTTGGCA GCAGCGGTTC TTTAGGAGCC
    ACACTGAGTT CAGCTCTTGG TGGATTTGGG ACTGCAGTTG CGAATTCCAA CACTGGCAGT
    GGATCTCGTC GTGACTCTCT GACTGGCAGC AGTGATATTT ACAAAAGAAC TTCTAGCAGT
    CTGACACCCA TAGGTCACAG CTTCTACAAT GGACTGGGCT TTTCATCTTC TCCAGGTCCT
    GTGGGAATGC CACTTCCCAG TCAGGGTCCC AATCACTCCC AAACACCACC TCCATCCCTG
    TCTTCTCATG GGTCCTCCAC CAGCCTCAAT CTTGGAGGTT TAACAAATGG CAGTGGGCGA
    TATATTTCTG CTGCACCAGG GGCAGAGGCA AAGTACAGAA GTGCCAGCAG TGCATCTAGC
    TTGTTTAGCC CAAGCAGCAC TCTCTTCCCA TCCTCCCGCT TGCGCTATGG AATGTCAGAT
    GTCATGCCCT CTGGACGAAG CAGACTACTT GAAGATTTCA GGAACAACCG CTACCCAAAT
    TTACAGTTGC GAGAAATTGC TGGCCACATT ATGGAGTTTT CCCAAGACCA GCATGGATCA
    AGGTTTATAC AGTTAAAGCT GGAGCGAGCT ACACCTGCAG AGAGACAACT GGTGTTCAAC
    GAGATCCTTC AAGCAGCTTA TCAACTTATG GTGGATGTAT TTGGGAATTA CGTCATACAG
    AAATTTTTTG AGTTTGGCAG CCTAGAACAG AAGCTTGCTC TTGCTGAACG GATTCGTGGG
    CATGTACTTT CACTGGCGCT ACAAATGTAT GGATGTCGGG TGATACAGAA GGCACTGGAG
    TTTATTCCCC CTGATCAGCA AGTTATTAAT GAAATGGTCA GAGAGTTGGA TGGTCACGTC
    TTGAAGTGTG TAAAGGACCA GAATGGGAAT CATGTGGTTC AAAAATGCAT AGAGTGTGTA
    CAGCCGCAGT CCCTTCAATT CATTATTGAT GCCTTCAAGA GCCAGGTATT TGCACTCTCT
    ACGCATCCGT ATGGCTGTCG AGTGATCCAG CGAATTTTGG AGCACTGCCT TCCGGAGCAG
    ACACTGCCCA TTTTAGAAGA GTTACACCAG CACACGGAAC AGCTTGTTCA GGATCAGTAT
    GGTAACTACG TTATCCAGCA TGTTTTGGAG CATGGTCGAC CGGAGGACAA AAGTAAAATT
    GTTGCAGAGA TTCGTGGAAA TGTTCTAGTC CTGAGTCAGC ACAAATTTGC CAGTAATGTA
    GTTGAAAAAT GTGTGACACA TGCGTCTCGT ACGGAAAGAG CCATGCTTAT AGACGAGGTG
    TGCACAATGA ATGATGGGCC TCATAGTGCC TTATACACCA TGATGAAGGA CCAGTACGCT
    AACTACGTTG TACAGAAAAT GATCGATGTA GCAGAACCAG CTCAGCGCAA GATTGTTATG
    CACAAGATCC GACCACATAT TGCCACTCTC CGTAAATACA CGTATGGCAA GCACATCCTG
    GCCAAGTTGG AGAAGTACTA CATGAAAAAC GGCATGGATC TGGGTCCAAT GTGTGGACCC
    TCAAATGGAA TCATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.