PUM1 cDNA ORF clone, Canis lupus familiaris(dog)

The following PUM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe286889 XM_022412404.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe286890 XM_022412408.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe286891 XM_022412410.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe286892 XM_022412416.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe286877 XM_022412370.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe286878 XM_022412371.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe286879 XM_022412372.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe286880 XM_022412376.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe286881 XM_022412380.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe286882 XM_022412382.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe286883 XM_022412383.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe286884 XM_022412387.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe286885 XM_022412389.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe286886 XM_022412392.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe286887 XM_022412393.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe286888 XM_022412399.1
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe35441 XM_014109998.1
Latest version!
Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe35442 XM_014110005.1
Latest version!
Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe35443 XM_014110008.1
Latest version!
Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe03438 XM_005617672.2
Latest version!
Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe03438 XM_014109987.2
Latest version!
Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe03439 XM_014109994.1
Latest version!
Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe03440 XM_005617675.2
Latest version!
Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe03441 XM_005617677.2
Latest version!
Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe03442 XM_860226.4
Latest version!
Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe03444 XM_005617679.2
Latest version!
Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe03444 XM_014110015.1
Latest version!
Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe03445 XM_005617680.2
Latest version!
Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe03438 XM_014109987.1 Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe286889
    Clone ID Related Accession (Same CDS sequence) XM_022412404.1
    Accession Version XM_022412404.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CTGTTCCAAA GACCTAATGC ACTTGCTGTC CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG
    TATGCACTGG CAGCGGCTCA TCAACCTCAC ATCGGTGTGT TTTCAGCAGG TTTAGCGCCC
    GCTGCGTTTG TCCCCAATCC ATACATCATC AGTGCTGCTC CCCCGGGGAC GGACCCCTAC
    ACAGCTGGAC TGGCTGCAGC GGCGACACTA GGCCCGGCTG TGGTCCCTCA CCAGTATTAT
    GGAGTCACTC CCTGGGGAGT CTACCCAGCC AGTCTCTTCC AGCAGCAAGC AGCTGCTGCT
    GCGGCAGCAA CTAATTCTGC AAATCAGCAG ACTACCCCAC AGGCTCAGCA AGGGCAGCAG
    CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT CCTTTGACCC CAAATCAGAA CCAGCAGGGA
    CAGCAAACAG ATCCTCTGGT GGCAGCTGCA GCAGTGAATT CTGCCCTTGC TTTTGGACAA
    GGACTGGCTG CAGGCATGCC AGGTTACCCG GTCTTGGCTC CTGCTGCTTA CTATGACCAA
    ACCGGCGCCC TTGTGGTGAA TGCAGGAGCG AGAAATGGTC TTGGCGCTCC TGTTCGGCTG
    GTAGCTCCTG CCCCAGTCAT TATCAGTTCC TCGGCTGCAC AAGCAGCTGT TGCGGCGGCC
    GCAGCTTCAG CAAATGGAGC AGCCGGTGGT CTTGCTGGAA CAACAAATGG ACCATTTCGC
    CCTTTAGGAA CCCAGCAGCC CCAGCCCCAG CCCCAGCAGC AGCCCAGTAA CAGCCTGGCA
    TCCAGCTCTT TCTATGGCAA CAACTCTCTG AGCAGCAATT CACAGAGCAG CTCCCTCTTC
    TCCCAGGGCT CTGCCCAGCC TGCCAACACA TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC
    GGCGCCACCC TGGGATCAGC CCTTGGAGGG TTTGGAACAG CAGTTGCAAA CTCCAACACT
    GGCAGTGGCT CCCGCCGTGA CTCCCTGACT GGCAGCAGTG ACCTTTATAA GAGGACATCG
    AGCAGCTTGA CCCCCATTGG ACACAGTTTT TATAACGGCC TTAGCTTTTC CTCCTCTCCT
    GGACCCGTGG GCATGCCTCT CCCTAGTCAG GGACCAGGAC ATTCACAGAC ACCACCTCCT
    TCCCTCTCTT CACATGGATC CTCTTCAAGC TTAAACCTGG GAGGCCTCAC CAATGGCAGT
    GGGAGATACA TCTCTGCCGC GCCAGGCGCT GAAGCCAAGT ACCGCAGCGC GAGCAGTGCC
    TCCAGCCTCT TCAGCCCCAG CAGCACCCTG TTTTCTTCTT CTCGGTTGCG ATATGGAATG
    TCTGATGTCA TGCCCTCTGG CAGGAGCAGG CTTCTGGAAG ATTTCCGAAA TAACCGGTAC
    CCCAATTTAC AACTGCGGGA GATTGCTGGG CATATAATGG AGTTTTCCCA AGACCAGCAT
    GGGTCCAGAT TCATTCAGCT GAAACTAGAA CGAGCCACAC CAGCTGAACG CCAGCTTGTC
    TTCAATGAAA TCCTCCAGGC CGCTTACCAG CTAATGGTGG ATGTATTTGG AAATTACGTC
    ATTCAAAAGT TCTTTGAATT TGGCAGCCTA GAACAGAAGC TGGCTTTGGC AGAACGGATT
    CGAGGCCACG TCCTATCATT GGCACTGCAG ATGTATGGCT GCCGTGTTAT CCAGAAAGCT
    CTTGAATTTA TTCCCTCAGA CCAGCAGGTA ATTAATGAGA TGGTTCGGGA GCTAGACGGC
    CACGTCCTGA AGTGTGTGAA AGACCAGAAT GGTAATCACG TGGTTCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTTT GCAGTTTATT ATTGATGCGT TTAAGGGGCA GGTATTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TCCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTATGTGAT TCAGCACGTA TTGGAGCATG GCCGTCCTGA GGACAAAAGT
    AAAATTGTAG CAGAAATCCG AGGCAATGTG CTTGTATTGA GTCAGCACAA ATTTGCAAGC
    AACGTTGTGG AGAAGTGTGT CACTCATGCG TCCCGCACAG AGCGTGCTGT GCTTATCGAC
    GAGGTATGCA CCATGAATGA CGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GCGCAAGATC
    GTCATGCATA AGATCCGGCC CCACATTGCG ACTCTCCGCA AATACACCTA TGGCAAGCAC
    ATTTTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGT
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268112.1
    CDS78..3041
    Translation

    Target ORF information:

    RefSeq Version XM_022412404.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412404.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CTGTTCCAAA GACCTAATGC ACTTGCTGTC CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG
    TATGCACTGG CAGCGGCTCA TCAACCTCAC ATCGGTGTGT TTTCAGCAGG TTTAGCGCCC
    GCTGCGTTTG TCCCCAATCC ATACATCATC AGTGCTGCTC CCCCGGGGAC GGACCCCTAC
    ACAGCTGGAC TGGCTGCAGC GGCGACACTA GGCCCGGCTG TGGTCCCTCA CCAGTATTAT
    GGAGTCACTC CCTGGGGAGT CTACCCAGCC AGTCTCTTCC AGCAGCAAGC AGCTGCTGCT
    GCGGCAGCAA CTAATTCTGC AAATCAGCAG ACTACCCCAC AGGCTCAGCA AGGGCAGCAG
    CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT CCTTTGACCC CAAATCAGAA CCAGCAGGGA
    CAGCAAACAG ATCCTCTGGT GGCAGCTGCA GCAGTGAATT CTGCCCTTGC TTTTGGACAA
    GGACTGGCTG CAGGCATGCC AGGTTACCCG GTCTTGGCTC CTGCTGCTTA CTATGACCAA
    ACCGGCGCCC TTGTGGTGAA TGCAGGAGCG AGAAATGGTC TTGGCGCTCC TGTTCGGCTG
    GTAGCTCCTG CCCCAGTCAT TATCAGTTCC TCGGCTGCAC AAGCAGCTGT TGCGGCGGCC
    GCAGCTTCAG CAAATGGAGC AGCCGGTGGT CTTGCTGGAA CAACAAATGG ACCATTTCGC
    CCTTTAGGAA CCCAGCAGCC CCAGCCCCAG CCCCAGCAGC AGCCCAGTAA CAGCCTGGCA
    TCCAGCTCTT TCTATGGCAA CAACTCTCTG AGCAGCAATT CACAGAGCAG CTCCCTCTTC
    TCCCAGGGCT CTGCCCAGCC TGCCAACACA TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC
    GGCGCCACCC TGGGATCAGC CCTTGGAGGG TTTGGAACAG CAGTTGCAAA CTCCAACACT
    GGCAGTGGCT CCCGCCGTGA CTCCCTGACT GGCAGCAGTG ACCTTTATAA GAGGACATCG
    AGCAGCTTGA CCCCCATTGG ACACAGTTTT TATAACGGCC TTAGCTTTTC CTCCTCTCCT
    GGACCCGTGG GCATGCCTCT CCCTAGTCAG GGACCAGGAC ATTCACAGAC ACCACCTCCT
    TCCCTCTCTT CACATGGATC CTCTTCAAGC TTAAACCTGG GAGGCCTCAC CAATGGCAGT
    GGGAGATACA TCTCTGCCGC GCCAGGCGCT GAAGCCAAGT ACCGCAGCGC GAGCAGTGCC
    TCCAGCCTCT TCAGCCCCAG CAGCACCCTG TTTTCTTCTT CTCGGTTGCG ATATGGAATG
    TCTGATGTCA TGCCCTCTGG CAGGAGCAGG CTTCTGGAAG ATTTCCGAAA TAACCGGTAC
    CCCAATTTAC AACTGCGGGA GATTGCTGGG CATATAATGG AGTTTTCCCA AGACCAGCAT
    GGGTCCAGAT TCATTCAGCT GAAACTAGAA CGAGCCACAC CAGCTGAACG CCAGCTTGTC
    TTCAATGAAA TCCTCCAGGC CGCTTACCAG CTAATGGTGG ATGTATTTGG AAATTACGTC
    ATTCAAAAGT TCTTTGAATT TGGCAGCCTA GAACAGAAGC TGGCTTTGGC AGAACGGATT
    CGAGGCCACG TCCTATCATT GGCACTGCAG ATGTATGGCT GCCGTGTTAT CCAGAAAGCT
    CTTGAATTTA TTCCCTCAGA CCAGCAGGTA ATTAATGAGA TGGTTCGGGA GCTAGACGGC
    CACGTCCTGA AGTGTGTGAA AGACCAGAAT GGTAATCACG TGGTTCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTTT GCAGTTTATT ATTGATGCGT TTAAGGGGCA GGTATTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TCCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTATGTGAT TCAGCACGTA TTGGAGCATG GCCGTCCTGA GGACAAAAGT
    AAAATTGTAG CAGAAATCCG AGGCAATGTG CTTGTATTGA GTCAGCACAA ATTTGCAAGC
    AACGTTGTGG AGAAGTGTGT CACTCATGCG TCCCGCACAG AGCGTGCTGT GCTTATCGAC
    GAGGTATGCA CCATGAATGA CGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GCGCAAGATC
    GTCATGCATA AGATCCGGCC CCACATTGCG ACTCTCCGCA AATACACCTA TGGCAAGCAC
    ATTTTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGT
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286890
    Clone ID Related Accession (Same CDS sequence) XM_022412408.1
    Accession Version XM_022412408.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CTGTTCCAAA GACCTAATGC ACTTGCTGTC CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG
    TATGCACTGG CAGCGGCTCA TCAACCTCAC ATCGCAGGTT TAGCGCCCGC TGCGTTTGTC
    CCCAATCCAT ACATCATCAG TGCTGCTCCC CCGGGGACGG ACCCCTACAC AGCTGGACTG
    GCTGCAGCGG CGACACTAGG CCCGGCTGTG GTCCCTCACC AGTATTATGG AGTCACTCCC
    TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG CAGCAAGCAG CTGCTGCTGC GGCAGCAACT
    AATTCTGCAA ATCAGCAGAC TACCCCACAG GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT
    GGAGGAGCCA GCCAGCGTCC TTTGACCCCA AATCAGAACC AGCAGGGACA GCAAACAGAT
    CCTCTGGTGG CAGCTGCAGC AGTGAATTCT GCCCTTGCTT TTGGACAAGG ACTGGCTGCA
    GGCATGCCAG GTTACCCGGT CTTGGCTCCT GCTGCTTACT ATGACCAAAC CGGCGCCCTT
    GTGGTGAATG CAGGAGCGAG AAATGGTCTT GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC
    CCAGTCATTA TCAGTTCCTC GGCTGCACAA GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA
    AATGGAGCAG CCGGTGGTCT TGCTGGAACA ACAAATGGAC CATTTCGCCC TTTAGGAACC
    CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG CCCAGTAACA GCCTGGCATC CAGCTCTTTC
    TATGGCAACA ACTCTCTGAG CAGCAATTCA CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT
    GCCCAGCCTG CCAACACATC CTTGGGATTT GGAAGTAGCA GTTCTCTCGG CGCCACCCTG
    GGATCAGCCC TTGGAGGGTT TGGAACAGCA GTTGCAAACT CCAACACTGG CAGTGGCTCC
    CGCCGTGACT CCCTGACTGG CAGCAGTGAC CTTTATAAGA GGACATCGAG CAGCTTGACC
    CCCATTGGAC ACAGTTTTTA TAACGGCCTT AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC
    ATGCCTCTCC CTAGTCAGGG ACCAGGACAT TCACAGACAC CACCTCCTTC CCTCTCTTCA
    CATGGATCCT CTTCAAGCTT AAACCTGGGA GGCCTCACCA ATGGCAGTGG GAGATACATC
    TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC
    AGCCCCAGCA GCACCCTGTT TTCTTCTTCT CGGTTGCGAT ATGGAATGTC TGATGTCATG
    CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT TTCCGAAATA ACCGGTACCC CAATTTACAA
    CTGCGGGAGA TTGCTGGGCA TATAATGGAG TTTTCCCAAG ACCAGCATGG GTCCAGATTC
    ATTCAGCTGA AACTAGAACG AGCCACACCA GCTGAACGCC AGCTTGTCTT CAATGAAATC
    CTCCAGGCCG CTTACCAGCT AATGGTGGAT GTATTTGGAA ATTACGTCAT TCAAAAGTTC
    TTTGAATTTG GCAGCCTAGA ACAGAAGCTG GCTTTGGCAG AACGGATTCG AGGCCACGTC
    CTATCATTGG CACTGCAGAT GTATGGCTGC CGTGTTATCC AGAAAGCTCT TGAATTTATT
    CCCTCAGACC AGCAGGTAAT TAATGAGATG GTTCGGGAGC TAGACGGCCA CGTCCTGAAG
    TGTGTGAAAG ACCAGAATGG TAATCACGTG GTTCAGAAGT GCATCGAATG TGTGCAGCCC
    CAGTCTTTGC AGTTTATTAT TGATGCGTTT AAGGGGCAGG TATTTGCTTT GTCCACGCAT
    CCTTATGGCT GCCGAGTGAT TCAGAGGATC CTGGAGCACT GTCTCCCTGA CCAGACACTC
    CCTATTTTAG AGGAGCTTCA CCAGCACACA GAGCAACTTG TACAGGATCA GTATGGAAAT
    TATGTGATTC AGCACGTATT GGAGCATGGC CGTCCTGAGG ACAAAAGTAA AATTGTAGCA
    GAAATCCGAG GCAATGTGCT TGTATTGAGT CAGCACAAAT TTGCAAGCAA CGTTGTGGAG
    AAGTGTGTCA CTCATGCGTC CCGCACAGAG CGTGCTGTGC TTATCGACGA GGTATGCACC
    ATGAATGACG GTCCCCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAACTAC
    GTGGTCCAGA AGATGATCGA TGTGGCAGAG CCAGCCCAGC GCAAGATCGT CATGCATAAG
    ATCCGGCCCC ACATTGCGAC TCTCCGCAAA TACACCTATG GCAAGCACAT TTTGGCCAAG
    CTGGAGAAAT ACTACATGAA GAATGGCGTT GACTTAGGGC CCATCTGTGG CCCCCCTAAT
    GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268116.1
    CDS77..3028
    Translation

    Target ORF information:

    RefSeq Version XM_022412408.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CTGTTCCAAA GACCTAATGC ACTTGCTGTC CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG
    TATGCACTGG CAGCGGCTCA TCAACCTCAC ATCGCAGGTT TAGCGCCCGC TGCGTTTGTC
    CCCAATCCAT ACATCATCAG TGCTGCTCCC CCGGGGACGG ACCCCTACAC AGCTGGACTG
    GCTGCAGCGG CGACACTAGG CCCGGCTGTG GTCCCTCACC AGTATTATGG AGTCACTCCC
    TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG CAGCAAGCAG CTGCTGCTGC GGCAGCAACT
    AATTCTGCAA ATCAGCAGAC TACCCCACAG GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT
    GGAGGAGCCA GCCAGCGTCC TTTGACCCCA AATCAGAACC AGCAGGGACA GCAAACAGAT
    CCTCTGGTGG CAGCTGCAGC AGTGAATTCT GCCCTTGCTT TTGGACAAGG ACTGGCTGCA
    GGCATGCCAG GTTACCCGGT CTTGGCTCCT GCTGCTTACT ATGACCAAAC CGGCGCCCTT
    GTGGTGAATG CAGGAGCGAG AAATGGTCTT GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC
    CCAGTCATTA TCAGTTCCTC GGCTGCACAA GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA
    AATGGAGCAG CCGGTGGTCT TGCTGGAACA ACAAATGGAC CATTTCGCCC TTTAGGAACC
    CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG CCCAGTAACA GCCTGGCATC CAGCTCTTTC
    TATGGCAACA ACTCTCTGAG CAGCAATTCA CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT
    GCCCAGCCTG CCAACACATC CTTGGGATTT GGAAGTAGCA GTTCTCTCGG CGCCACCCTG
    GGATCAGCCC TTGGAGGGTT TGGAACAGCA GTTGCAAACT CCAACACTGG CAGTGGCTCC
    CGCCGTGACT CCCTGACTGG CAGCAGTGAC CTTTATAAGA GGACATCGAG CAGCTTGACC
    CCCATTGGAC ACAGTTTTTA TAACGGCCTT AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC
    ATGCCTCTCC CTAGTCAGGG ACCAGGACAT TCACAGACAC CACCTCCTTC CCTCTCTTCA
    CATGGATCCT CTTCAAGCTT AAACCTGGGA GGCCTCACCA ATGGCAGTGG GAGATACATC
    TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC
    AGCCCCAGCA GCACCCTGTT TTCTTCTTCT CGGTTGCGAT ATGGAATGTC TGATGTCATG
    CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT TTCCGAAATA ACCGGTACCC CAATTTACAA
    CTGCGGGAGA TTGCTGGGCA TATAATGGAG TTTTCCCAAG ACCAGCATGG GTCCAGATTC
    ATTCAGCTGA AACTAGAACG AGCCACACCA GCTGAACGCC AGCTTGTCTT CAATGAAATC
    CTCCAGGCCG CTTACCAGCT AATGGTGGAT GTATTTGGAA ATTACGTCAT TCAAAAGTTC
    TTTGAATTTG GCAGCCTAGA ACAGAAGCTG GCTTTGGCAG AACGGATTCG AGGCCACGTC
    CTATCATTGG CACTGCAGAT GTATGGCTGC CGTGTTATCC AGAAAGCTCT TGAATTTATT
    CCCTCAGACC AGCAGGTAAT TAATGAGATG GTTCGGGAGC TAGACGGCCA CGTCCTGAAG
    TGTGTGAAAG ACCAGAATGG TAATCACGTG GTTCAGAAGT GCATCGAATG TGTGCAGCCC
    CAGTCTTTGC AGTTTATTAT TGATGCGTTT AAGGGGCAGG TATTTGCTTT GTCCACGCAT
    CCTTATGGCT GCCGAGTGAT TCAGAGGATC CTGGAGCACT GTCTCCCTGA CCAGACACTC
    CCTATTTTAG AGGAGCTTCA CCAGCACACA GAGCAACTTG TACAGGATCA GTATGGAAAT
    TATGTGATTC AGCACGTATT GGAGCATGGC CGTCCTGAGG ACAAAAGTAA AATTGTAGCA
    GAAATCCGAG GCAATGTGCT TGTATTGAGT CAGCACAAAT TTGCAAGCAA CGTTGTGGAG
    AAGTGTGTCA CTCATGCGTC CCGCACAGAG CGTGCTGTGC TTATCGACGA GGTATGCACC
    ATGAATGACG GTCCCCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAACTAC
    GTGGTCCAGA AGATGATCGA TGTGGCAGAG CCAGCCCAGC GCAAGATCGT CATGCATAAG
    ATCCGGCCCC ACATTGCGAC TCTCCGCAAA TACACCTATG GCAAGCACAT TTTGGCCAAG
    CTGGAGAAAT ACTACATGAA GAATGGCGTT GACTTAGGGC CCATCTGTGG CCCCCCTAAT
    GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286891
    Clone ID Related Accession (Same CDS sequence) XM_022412410.1
    Accession Version XM_022412410.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CTGTTCCAAA GACCTAATGC ACTTGCTGTC CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG
    TATGCACTGG CAGCGGCTCA TCAACCTCAC ATCGGTTTAG CGCCCGCTGC GTTTGTCCCC
    AATCCATACA TCATCAGTGC TGCTCCCCCG GGGACGGACC CCTACACAGC TGGACTGGCT
    GCAGCGGCGA CACTAGGCCC GGCTGTGGTC CCTCACCAGT ATTATGGAGT CACTCCCTGG
    GGAGTCTACC CAGCCAGTCT CTTCCAGCAG CAAGCAGCTG CTGCTGCGGC AGCAACTAAT
    TCTGCAAATC AGCAGACTAC CCCACAGGCT CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA
    GGAGCCAGCC AGCGTCCTTT GACCCCAAAT CAGAACCAGC AGGGACAGCA AACAGATCCT
    CTGGTGGCAG CTGCAGCAGT GAATTCTGCC CTTGCTTTTG GACAAGGACT GGCTGCAGGC
    ATGCCAGGTT ACCCGGTCTT GGCTCCTGCT GCTTACTATG ACCAAACCGG CGCCCTTGTG
    GTGAATGCAG GAGCGAGAAA TGGTCTTGGC GCTCCTGTTC GGCTGGTAGC TCCTGCCCCA
    GTCATTATCA GTTCCTCGGC TGCACAAGCA GCTGTTGCGG CGGCCGCAGC TTCAGCAAAT
    GGAGCAGCCG GTGGTCTTGC TGGAACAACA AATGGACCAT TTCGCCCTTT AGGAACCCAG
    CAGCCCCAGC CCCAGCCCCA GCAGCAGCCC AGTAACAGCC TGGCATCCAG CTCTTTCTAT
    GGCAACAACT CTCTGAGCAG CAATTCACAG AGCAGCTCCC TCTTCTCCCA GGGCTCTGCC
    CAGCCTGCCA ACACATCCTT GGGATTTGGA AGTAGCAGTT CTCTCGGCGC CACCCTGGGA
    TCAGCCCTTG GAGGGTTTGG AACAGCAGTT GCAAACTCCA ACACTGGCAG TGGCTCCCGC
    CGTGACTCCC TGACTGGCAG CAGTGACCTT TATAAGAGGA CATCGAGCAG CTTGACCCCC
    ATTGGACACA GTTTTTATAA CGGCCTTAGC TTTTCCTCCT CTCCTGGACC CGTGGGCATG
    CCTCTCCCTA GTCAGGGACC AGGACATTCA CAGACACCAC CTCCTTCCCT CTCTTCACAT
    GGATCCTCTT CAAGCTTAAA CCTGGGAGGC CTCACCAATG GCAGTGGGAG ATACATCTCT
    GCCGCGCCAG GCGCTGAAGC CAAGTACCGC AGCGCGAGCA GTGCCTCCAG CCTCTTCAGC
    CCCAGCAGCA CCCTGTTTTC TTCTTCTCGG TTGCGATATG GAATGTCTGA TGTCATGCCC
    TCTGGCAGGA GCAGGCTTCT GGAAGATTTC CGAAATAACC GGTACCCCAA TTTACAACTG
    CGGGAGATTG CTGGGCATAT AATGGAGTTT TCCCAAGACC AGCATGGGTC CAGATTCATT
    CAGCTGAAAC TAGAACGAGC CACACCAGCT GAACGCCAGC TTGTCTTCAA TGAAATCCTC
    CAGGCCGCTT ACCAGCTAAT GGTGGATGTA TTTGGAAATT ACGTCATTCA AAAGTTCTTT
    GAATTTGGCA GCCTAGAACA GAAGCTGGCT TTGGCAGAAC GGATTCGAGG CCACGTCCTA
    TCATTGGCAC TGCAGATGTA TGGCTGCCGT GTTATCCAGA AAGCTCTTGA ATTTATTCCC
    TCAGACCAGC AGGTAATTAA TGAGATGGTT CGGGAGCTAG ACGGCCACGT CCTGAAGTGT
    GTGAAAGACC AGAATGGTAA TCACGTGGTT CAGAAGTGCA TCGAATGTGT GCAGCCCCAG
    TCTTTGCAGT TTATTATTGA TGCGTTTAAG GGGCAGGTAT TTGCTTTGTC CACGCATCCT
    TATGGCTGCC GAGTGATTCA GAGGATCCTG GAGCACTGTC TCCCTGACCA GACACTCCCT
    ATTTTAGAGG AGCTTCACCA GCACACAGAG CAACTTGTAC AGGATCAGTA TGGAAATTAT
    GTGATTCAGC ACGTATTGGA GCATGGCCGT CCTGAGGACA AAAGTAAAAT TGTAGCAGAA
    ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG CACAAATTTG CAAGCAACGT TGTGGAGAAG
    TGTGTCACTC ATGCGTCCCG CACAGAGCGT GCTGTGCTTA TCGACGAGGT ATGCACCATG
    AATGACGGTC CCCACAGTGC CTTATACACC ATGATGAAGG ACCAGTATGC CAACTACGTG
    GTCCAGAAGA TGATCGATGT GGCAGAGCCA GCCCAGCGCA AGATCGTCAT GCATAAGATC
    CGGCCCCACA TTGCGACTCT CCGCAAATAC ACCTATGGCA AGCACATTTT GGCCAAGCTG
    GAGAAATACT ACATGAAGAA TGGCGTTGAC TTAGGGCCCA TCTGTGGCCC CCCTAATGGT
    ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268118.1
    CDS77..3025
    Translation

    Target ORF information:

    RefSeq Version XM_022412410.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412410.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CTGTTCCAAA GACCTAATGC ACTTGCTGTC CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG
    TATGCACTGG CAGCGGCTCA TCAACCTCAC ATCGGTTTAG CGCCCGCTGC GTTTGTCCCC
    AATCCATACA TCATCAGTGC TGCTCCCCCG GGGACGGACC CCTACACAGC TGGACTGGCT
    GCAGCGGCGA CACTAGGCCC GGCTGTGGTC CCTCACCAGT ATTATGGAGT CACTCCCTGG
    GGAGTCTACC CAGCCAGTCT CTTCCAGCAG CAAGCAGCTG CTGCTGCGGC AGCAACTAAT
    TCTGCAAATC AGCAGACTAC CCCACAGGCT CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA
    GGAGCCAGCC AGCGTCCTTT GACCCCAAAT CAGAACCAGC AGGGACAGCA AACAGATCCT
    CTGGTGGCAG CTGCAGCAGT GAATTCTGCC CTTGCTTTTG GACAAGGACT GGCTGCAGGC
    ATGCCAGGTT ACCCGGTCTT GGCTCCTGCT GCTTACTATG ACCAAACCGG CGCCCTTGTG
    GTGAATGCAG GAGCGAGAAA TGGTCTTGGC GCTCCTGTTC GGCTGGTAGC TCCTGCCCCA
    GTCATTATCA GTTCCTCGGC TGCACAAGCA GCTGTTGCGG CGGCCGCAGC TTCAGCAAAT
    GGAGCAGCCG GTGGTCTTGC TGGAACAACA AATGGACCAT TTCGCCCTTT AGGAACCCAG
    CAGCCCCAGC CCCAGCCCCA GCAGCAGCCC AGTAACAGCC TGGCATCCAG CTCTTTCTAT
    GGCAACAACT CTCTGAGCAG CAATTCACAG AGCAGCTCCC TCTTCTCCCA GGGCTCTGCC
    CAGCCTGCCA ACACATCCTT GGGATTTGGA AGTAGCAGTT CTCTCGGCGC CACCCTGGGA
    TCAGCCCTTG GAGGGTTTGG AACAGCAGTT GCAAACTCCA ACACTGGCAG TGGCTCCCGC
    CGTGACTCCC TGACTGGCAG CAGTGACCTT TATAAGAGGA CATCGAGCAG CTTGACCCCC
    ATTGGACACA GTTTTTATAA CGGCCTTAGC TTTTCCTCCT CTCCTGGACC CGTGGGCATG
    CCTCTCCCTA GTCAGGGACC AGGACATTCA CAGACACCAC CTCCTTCCCT CTCTTCACAT
    GGATCCTCTT CAAGCTTAAA CCTGGGAGGC CTCACCAATG GCAGTGGGAG ATACATCTCT
    GCCGCGCCAG GCGCTGAAGC CAAGTACCGC AGCGCGAGCA GTGCCTCCAG CCTCTTCAGC
    CCCAGCAGCA CCCTGTTTTC TTCTTCTCGG TTGCGATATG GAATGTCTGA TGTCATGCCC
    TCTGGCAGGA GCAGGCTTCT GGAAGATTTC CGAAATAACC GGTACCCCAA TTTACAACTG
    CGGGAGATTG CTGGGCATAT AATGGAGTTT TCCCAAGACC AGCATGGGTC CAGATTCATT
    CAGCTGAAAC TAGAACGAGC CACACCAGCT GAACGCCAGC TTGTCTTCAA TGAAATCCTC
    CAGGCCGCTT ACCAGCTAAT GGTGGATGTA TTTGGAAATT ACGTCATTCA AAAGTTCTTT
    GAATTTGGCA GCCTAGAACA GAAGCTGGCT TTGGCAGAAC GGATTCGAGG CCACGTCCTA
    TCATTGGCAC TGCAGATGTA TGGCTGCCGT GTTATCCAGA AAGCTCTTGA ATTTATTCCC
    TCAGACCAGC AGGTAATTAA TGAGATGGTT CGGGAGCTAG ACGGCCACGT CCTGAAGTGT
    GTGAAAGACC AGAATGGTAA TCACGTGGTT CAGAAGTGCA TCGAATGTGT GCAGCCCCAG
    TCTTTGCAGT TTATTATTGA TGCGTTTAAG GGGCAGGTAT TTGCTTTGTC CACGCATCCT
    TATGGCTGCC GAGTGATTCA GAGGATCCTG GAGCACTGTC TCCCTGACCA GACACTCCCT
    ATTTTAGAGG AGCTTCACCA GCACACAGAG CAACTTGTAC AGGATCAGTA TGGAAATTAT
    GTGATTCAGC ACGTATTGGA GCATGGCCGT CCTGAGGACA AAAGTAAAAT TGTAGCAGAA
    ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG CACAAATTTG CAAGCAACGT TGTGGAGAAG
    TGTGTCACTC ATGCGTCCCG CACAGAGCGT GCTGTGCTTA TCGACGAGGT ATGCACCATG
    AATGACGGTC CCCACAGTGC CTTATACACC ATGATGAAGG ACCAGTATGC CAACTACGTG
    GTCCAGAAGA TGATCGATGT GGCAGAGCCA GCCCAGCGCA AGATCGTCAT GCATAAGATC
    CGGCCCCACA TTGCGACTCT CCGCAAATAC ACCTATGGCA AGCACATTTT GGCCAAGCTG
    GAGAAATACT ACATGAAGAA TGGCGTTGAC TTAGGGCCCA TCTGTGGCCC CCCTAATGGT
    ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286892
    Clone ID Related Accession (Same CDS sequence) XM_022412416.1
    Accession Version XM_022412416.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CTGTTCCAAA GACCTAATGC ACTTGCTGTC CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG
    TATGCACTGG CAGCGGCTCA TCAACCTCAC ATCGGTTTAG CGCCCGCTGC GTTTGTCCCC
    AATCCATACA TCATCAGTGC TGCTCCCCCG GGGACGGACC CCTACACAGC TGGACTGGCT
    GCAGCGGCGA CACTAGGCCC GGCTGTGGTC CCTCACCAGT ATTATGGAGT CACTCCCTGG
    GGAGTCTACC CAGCCAGTCT CTTCCAGCAG CAAGCAGCTG CTGCTGCGGC AGCAACTAAT
    TCTGCAAATC AGCAGACTAC CCCACAGGCT CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA
    GGAGCCAGCC AGCGTCCTTT GACCCCAAAT CAGAACCAGC AGGGACAGCA AACAGATCCT
    CTGGTGGCAG CTGCAGCAGT GAATTCTGCC CTTGCTTTTG GACAAGGACT GGCTGCAGGC
    ATGCCAGGTT ACCCGGTCTT GGCTCCTGCT GCTTACTATG ACCAAACCGG CGCCCTTGTG
    GTGAATGCAG GAGCGAGAAA TGGTCTTGGC GCTCCTGTTC GGCTGGTAGC TCCTGCCCCA
    GTCATTATCA GTTCCTCGGC TGCACAAGCA GCTGTTGCGG CGGCCGCAGC TTCAGCAAAT
    GGAGCAGCCG GTGGTCTTGC TGGAACAACA AATGGACCAT TTCGCCCTTT AGGAACCCAG
    CAGCCCCAGC CCCAGCCCCA GCAGCAGCCC AGTAACAGCC TGGCATCCAG CTCTTTCTAT
    GGCAACAACT CTCTGAGCAG CAATTCACAG AGCAGCTCCC TCTTCTCCCA GGGCTCTGCC
    CAGCCTGCCA ACACATCCTT GGGATTTGGA AGTAGCAGTT CTCTCGGCGC CACCCTGGGA
    TCAGCCCTTG GAGGGTTTGG AACAGCAGTT GCAAACTCCA ACACTGGCAG TGGCTCCCGC
    CGTGACTCCC TGACTGGCAG CAGTGACCTT TATAAGAGGA CATCGAGCAG CTTGACCCCC
    ATTGGACACA GTTTTTATAA CGGCCTTAGC TTTTCCTCCT CTCCTGGACC CGTGGGCATG
    CCTCTCCCTA GTCAGGGACC AGGACATTCA CAGACACCAC CTCCTTCCCT CTCTTCACAT
    GGATCCTCTT CAAGCTTAAA CCTGGGAGGC CTCACCAATG GCAGTGGGAG ATACATCTCT
    GCCGCGCCAG GCGCTGAAGC CAAGTACCGC AGCGCGAGCA GTGCCTCCAG CCTCTTCAGC
    CCCAGCAGCA CCCTGTTTTC TTCTTCTCGG TTGCGATATG GAATGTCTGA TGTCATGCCC
    TCTGGCAGGA GCAGGCTTCT GGAAGATTTC CGAAATAACC GGTACCCCAA TTTACAACTG
    CGGGAGATTG CTGGGCATAT AATGGAGTTT TCCCAAGACC AGCATGGGTC CAGATTCATT
    CAGCTGAAAC TAGAACGAGC CACACCAGCT GAACGCCAGC TTGTCTTCAA TGAAATCCTC
    CAGGCCGCTT ACCAGCTAAT GGTGGATGTA TTTGGAAATT ACGTCATTCA AAAGTTCTTT
    GAATTTGGCA GCCTAGAACA GAAGCTGGCT TTGGCAGAAC GGATTCGAGG CCACGTCCTA
    TCATTGGCAC TGCAGATGTA TGGCTGCCGT GTTATCCAGA AAGCTCTTGA ATTTATTCCC
    TCAGACCAGC AGAATGAGAT GGTTCGGGAG CTAGACGGCC ACGTCCTGAA GTGTGTGAAA
    GACCAGAATG GTAATCACGT GGTTCAGAAG TGCATCGAAT GTGTGCAGCC CCAGTCTTTG
    CAGTTTATTA TTGATGCGTT TAAGGGGCAG GTATTTGCTT TGTCCACGCA TCCTTATGGC
    TGCCGAGTGA TTCAGAGGAT CCTGGAGCAC TGTCTCCCTG ACCAGACACT CCCTATTTTA
    GAGGAGCTTC ACCAGCACAC AGAGCAACTT GTACAGGATC AGTATGGAAA TTATGTGATT
    CAGCACGTAT TGGAGCATGG CCGTCCTGAG GACAAAAGTA AAATTGTAGC AGAAATCCGA
    GGCAATGTGC TTGTATTGAG TCAGCACAAA TTTGCAAGCA ACGTTGTGGA GAAGTGTGTC
    ACTCATGCGT CCCGCACAGA GCGTGCTGTG CTTATCGACG AGGTATGCAC CATGAATGAC
    GGTCCCCACA GTGCCTTATA CACCATGATG AAGGACCAGT ATGCCAACTA CGTGGTCCAG
    AAGATGATCG ATGTGGCAGA GCCAGCCCAG CGCAAGATCG TCATGCATAA GATCCGGCCC
    CACATTGCGA CTCTCCGCAA ATACACCTAT GGCAAGCACA TTTTGGCCAA GCTGGAGAAA
    TACTACATGA AGAATGGCGT TGACTTAGGG CCCATCTGTG GCCCCCCTAA TGGTATTATC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268124.1
    CDS77..3019
    Translation

    Target ORF information:

    RefSeq Version XM_022412416.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CTGTTCCAAA GACCTAATGC ACTTGCTGTC CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG
    TATGCACTGG CAGCGGCTCA TCAACCTCAC ATCGGTTTAG CGCCCGCTGC GTTTGTCCCC
    AATCCATACA TCATCAGTGC TGCTCCCCCG GGGACGGACC CCTACACAGC TGGACTGGCT
    GCAGCGGCGA CACTAGGCCC GGCTGTGGTC CCTCACCAGT ATTATGGAGT CACTCCCTGG
    GGAGTCTACC CAGCCAGTCT CTTCCAGCAG CAAGCAGCTG CTGCTGCGGC AGCAACTAAT
    TCTGCAAATC AGCAGACTAC CCCACAGGCT CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA
    GGAGCCAGCC AGCGTCCTTT GACCCCAAAT CAGAACCAGC AGGGACAGCA AACAGATCCT
    CTGGTGGCAG CTGCAGCAGT GAATTCTGCC CTTGCTTTTG GACAAGGACT GGCTGCAGGC
    ATGCCAGGTT ACCCGGTCTT GGCTCCTGCT GCTTACTATG ACCAAACCGG CGCCCTTGTG
    GTGAATGCAG GAGCGAGAAA TGGTCTTGGC GCTCCTGTTC GGCTGGTAGC TCCTGCCCCA
    GTCATTATCA GTTCCTCGGC TGCACAAGCA GCTGTTGCGG CGGCCGCAGC TTCAGCAAAT
    GGAGCAGCCG GTGGTCTTGC TGGAACAACA AATGGACCAT TTCGCCCTTT AGGAACCCAG
    CAGCCCCAGC CCCAGCCCCA GCAGCAGCCC AGTAACAGCC TGGCATCCAG CTCTTTCTAT
    GGCAACAACT CTCTGAGCAG CAATTCACAG AGCAGCTCCC TCTTCTCCCA GGGCTCTGCC
    CAGCCTGCCA ACACATCCTT GGGATTTGGA AGTAGCAGTT CTCTCGGCGC CACCCTGGGA
    TCAGCCCTTG GAGGGTTTGG AACAGCAGTT GCAAACTCCA ACACTGGCAG TGGCTCCCGC
    CGTGACTCCC TGACTGGCAG CAGTGACCTT TATAAGAGGA CATCGAGCAG CTTGACCCCC
    ATTGGACACA GTTTTTATAA CGGCCTTAGC TTTTCCTCCT CTCCTGGACC CGTGGGCATG
    CCTCTCCCTA GTCAGGGACC AGGACATTCA CAGACACCAC CTCCTTCCCT CTCTTCACAT
    GGATCCTCTT CAAGCTTAAA CCTGGGAGGC CTCACCAATG GCAGTGGGAG ATACATCTCT
    GCCGCGCCAG GCGCTGAAGC CAAGTACCGC AGCGCGAGCA GTGCCTCCAG CCTCTTCAGC
    CCCAGCAGCA CCCTGTTTTC TTCTTCTCGG TTGCGATATG GAATGTCTGA TGTCATGCCC
    TCTGGCAGGA GCAGGCTTCT GGAAGATTTC CGAAATAACC GGTACCCCAA TTTACAACTG
    CGGGAGATTG CTGGGCATAT AATGGAGTTT TCCCAAGACC AGCATGGGTC CAGATTCATT
    CAGCTGAAAC TAGAACGAGC CACACCAGCT GAACGCCAGC TTGTCTTCAA TGAAATCCTC
    CAGGCCGCTT ACCAGCTAAT GGTGGATGTA TTTGGAAATT ACGTCATTCA AAAGTTCTTT
    GAATTTGGCA GCCTAGAACA GAAGCTGGCT TTGGCAGAAC GGATTCGAGG CCACGTCCTA
    TCATTGGCAC TGCAGATGTA TGGCTGCCGT GTTATCCAGA AAGCTCTTGA ATTTATTCCC
    TCAGACCAGC AGAATGAGAT GGTTCGGGAG CTAGACGGCC ACGTCCTGAA GTGTGTGAAA
    GACCAGAATG GTAATCACGT GGTTCAGAAG TGCATCGAAT GTGTGCAGCC CCAGTCTTTG
    CAGTTTATTA TTGATGCGTT TAAGGGGCAG GTATTTGCTT TGTCCACGCA TCCTTATGGC
    TGCCGAGTGA TTCAGAGGAT CCTGGAGCAC TGTCTCCCTG ACCAGACACT CCCTATTTTA
    GAGGAGCTTC ACCAGCACAC AGAGCAACTT GTACAGGATC AGTATGGAAA TTATGTGATT
    CAGCACGTAT TGGAGCATGG CCGTCCTGAG GACAAAAGTA AAATTGTAGC AGAAATCCGA
    GGCAATGTGC TTGTATTGAG TCAGCACAAA TTTGCAAGCA ACGTTGTGGA GAAGTGTGTC
    ACTCATGCGT CCCGCACAGA GCGTGCTGTG CTTATCGACG AGGTATGCAC CATGAATGAC
    GGTCCCCACA GTGCCTTATA CACCATGATG AAGGACCAGT ATGCCAACTA CGTGGTCCAG
    AAGATGATCG ATGTGGCAGA GCCAGCCCAG CGCAAGATCG TCATGCATAA GATCCGGCCC
    CACATTGCGA CTCTCCGCAA ATACACCTAT GGCAAGCACA TTTTGGCCAA GCTGGAGAAA
    TACTACATGA AGAATGGCGT TGACTTAGGG CCCATCTGTG GCCCCCCTAA TGGTATTATC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286877
    Clone ID Related Accession (Same CDS sequence) XM_022412370.1
    Accession Version XM_022412370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTGTGTTTTC AGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGCTGTTGCG
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACCCA GCAGCCCCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAGC
    CTGGCATCCA GCTCTTTCTA TGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCTGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG CCTCACCAAT
    GGCAGTGGGA GATACATCTC TGCCGCGCCA GGCGCTGAAG CCAAGTACCG CAGCGCGAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTGTTTT CTTCTTCTCG GTTGCGATAT
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTC TGGAAGATTT CCGAAATAAC
    CGGTACCCCA ATTTACAACT GCGGGAGATT GCTGGGCATA TAATGGAGTT TTCCCAAGAC
    CAGCATGGGT CCAGATTCAT TCAGCTGAAA CTAGAACGAG CCACACCAGC TGAACGCCAG
    CTTGTCTTCA ATGAAATCCT CCAGGCCGCT TACCAGCTAA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AAAAGTTCTT TGAATTTGGC AGCCTAGAAC AGAAGCTGGC TTTGGCAGAA
    CGGATTCGAG GCCACGTCCT ATCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AATTTATTCC CTCAGACCAG CAGGTAATTA ATGAGATGGT TCGGGAGCTA
    GACGGCCACG TCCTGAAGTG TGTGAAAGAC CAGAATGGTA ATCACGTGGT TCAGAAGTGC
    ATCGAATGTG TGCAGCCCCA GTCTTTGCAG TTTATTATTG ATGCGTTTAA GGGGCAGGTA
    TTTGCTTTGT CCACGCATCC TTATGGCTGC CGAGTGATTC AGAGGATCCT GGAGCACTGT
    CTCCCTGACC AGACACTCCC TATTTTAGAG GAGCTTCACC AGCACACAGA GCAACTTGTA
    CAGGATCAGT ATGGAAATTA TGTGATTCAG CACGTATTGG AGCATGGCCG TCCTGAGGAC
    AAAAGTAAAA TTGTAGCAGA AATCCGAGGC AATGTGCTTG TATTGAGTCA GCACAAATTT
    GCAAGCAACG TTGTGGAGAA GTGTGTCACT CATGCGTCCC GCACAGAGCG TGCTGTGCTT
    ATCGACGAGG TATGCACCAT GAATGACGGT CCCCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAACTACGT GGTCCAGAAG ATGATCGATG TGGCAGAGCC AGCCCAGCGC
    AAGATCGTCA TGCATAAGAT CCGGCCCCAC ATTGCGACTC TCCGCAAATA CACCTATGGC
    AAGCACATTT TGGCCAAGCT GGAGAAATAC TACATGAAGA ATGGCGTTGA CTTAGGGCCC
    ATCTGTGGCC CCCCTAATGG TATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268078.1
    CDS79..3768
    Translation

    Target ORF information:

    RefSeq Version XM_022412370.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTGTGTTTTC AGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGCTGTTGCG
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACCCA GCAGCCCCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAGC
    CTGGCATCCA GCTCTTTCTA TGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCTGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG CCTCACCAAT
    GGCAGTGGGA GATACATCTC TGCCGCGCCA GGCGCTGAAG CCAAGTACCG CAGCGCGAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTGTTTT CTTCTTCTCG GTTGCGATAT
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTC TGGAAGATTT CCGAAATAAC
    CGGTACCCCA ATTTACAACT GCGGGAGATT GCTGGGCATA TAATGGAGTT TTCCCAAGAC
    CAGCATGGGT CCAGATTCAT TCAGCTGAAA CTAGAACGAG CCACACCAGC TGAACGCCAG
    CTTGTCTTCA ATGAAATCCT CCAGGCCGCT TACCAGCTAA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AAAAGTTCTT TGAATTTGGC AGCCTAGAAC AGAAGCTGGC TTTGGCAGAA
    CGGATTCGAG GCCACGTCCT ATCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AATTTATTCC CTCAGACCAG CAGGTAATTA ATGAGATGGT TCGGGAGCTA
    GACGGCCACG TCCTGAAGTG TGTGAAAGAC CAGAATGGTA ATCACGTGGT TCAGAAGTGC
    ATCGAATGTG TGCAGCCCCA GTCTTTGCAG TTTATTATTG ATGCGTTTAA GGGGCAGGTA
    TTTGCTTTGT CCACGCATCC TTATGGCTGC CGAGTGATTC AGAGGATCCT GGAGCACTGT
    CTCCCTGACC AGACACTCCC TATTTTAGAG GAGCTTCACC AGCACACAGA GCAACTTGTA
    CAGGATCAGT ATGGAAATTA TGTGATTCAG CACGTATTGG AGCATGGCCG TCCTGAGGAC
    AAAAGTAAAA TTGTAGCAGA AATCCGAGGC AATGTGCTTG TATTGAGTCA GCACAAATTT
    GCAAGCAACG TTGTGGAGAA GTGTGTCACT CATGCGTCCC GCACAGAGCG TGCTGTGCTT
    ATCGACGAGG TATGCACCAT GAATGACGGT CCCCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAACTACGT GGTCCAGAAG ATGATCGATG TGGCAGAGCC AGCCCAGCGC
    AAGATCGTCA TGCATAAGAT CCGGCCCCAC ATTGCGACTC TCCGCAAATA CACCTATGGC
    AAGCACATTT TGGCCAAGCT GGAGAAATAC TACATGAAGA ATGGCGTTGA CTTAGGGCCC
    ATCTGTGGCC CCCCTAATGG TATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286878
    Clone ID Related Accession (Same CDS sequence) XM_022412371.1
    Accession Version XM_022412371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CTCTTGCCAG GTAAAAAGTT TTGGGAAACA GATGAATCCA GCAAAGATGG ACCAAAAGGA
    ATATTCCTGG GTGATCAATG GCGAGACAGT GCCTGGGGAA CATCAGATCA TTCAGTTTCC
    CAGCCAATCA TGGTGCAGAG AAGACCTGGT CAGAGTTTCC ATGTGAACAG TGAGGTCAAT
    TCTGTACTGT CCCCACGATC GGAGAGTGGG GGACTAGGCG TTAGCATGGT GGAGTATGTG
    TTGAGCTCAT CCCCGGGCGA TTCCTGTCTA AGAAAAGGAG GATTTGGCCC AAGGGATGCA
    GACAGTGATG AAAACGACAA AGGTGACAAG AAGAACAAGG GTGCGTTTGA TGGAGATAAG
    CTAGGAGATT TGAAGGAGGA GGGTGATGTG ATGGACAAGA CGAATGGTTT GCCAGTGCAG
    AATGGGATTG ATGCAGACGT CAAAGATTTT AGCCGTACCC CTGGTAATTG CCAGAACTCT
    GCTAATGAAG TGGATCTTCT GGGTCCAAAC CAGAATGGTT CTGAGGGCTT AGCCCAGCTG
    ACCAGCACCA ATGGTGCCAA GCCTGTGGAG GATTTCTCCA ACATGGAGTC CCAGAGTGTC
    CCCTTGGACC CCATGGAACA TGTGGGCATG GAGCCTCTTC AGTTTGATTA TTCAGGCACG
    CAGGTACCTG TGGACTCAGC AGCAGCAACT GTGGGACTTT TTGACTACAA TTCTCAACAA
    CAGCTGTTCC AAAGACCTAA TGCACTTGCT GTCCAGCAGT TGACAGCTGC CCAGCAGCAG
    CAGTATGCAC TGGCAGCGGC TCATCAACCT CACATCGGTG TGTTTTCAGC AGGTTTAGCG
    CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGTGCTG CTCCCCCGGG GACGGACCCC
    TACACAGCTG GACTGGCTGC AGCGGCGACA CTAGGCCCGG CTGTGGTCCC TCACCAGTAT
    TATGGAGTCA CTCCCTGGGG AGTCTACCCA GCCAGTCTCT TCCAGCAGCA AGCAGCTGCT
    GCTGCGGCAG CAACTAATTC TGCAAATCAG CAGACTACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CCCCAAATCA GAACCAGCAG
    GGACAGCAAA CAGATCCTCT GGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCTTTTGGA
    CAAGGACTGG CTGCAGGCAT GCCAGGTTAC CCGGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGCG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGCGC TCCTGTTCGG
    CTGGTAGCTC CTGCCCCAGT CATTATCAGT TCCTCGGCTG CACAAGCAGC TGTTGCGGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACCCAGCA GCCCCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAGCCTG
    GCATCCAGCT CTTTCTATGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGCCT CACCAATGGC
    AGTGGGAGAT ACATCTCTGC CGCGCCAGGC GCTGAAGCCA AGTACCGCAG CGCGAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTGTTTTCTT CTTCTCGGTT GCGATATGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTCTGG AAGATTTCCG AAATAACCGG
    TACCCCAATT TACAACTGCG GGAGATTGCT GGGCATATAA TGGAGTTTTC CCAAGACCAG
    CATGGGTCCA GATTCATTCA GCTGAAACTA GAACGAGCCA CACCAGCTGA ACGCCAGCTT
    GTCTTCAATG AAATCCTCCA GGCCGCTTAC CAGCTAATGG TGGATGTATT TGGAAATTAC
    GTCATTCAAA AGTTCTTTGA ATTTGGCAGC CTAGAACAGA AGCTGGCTTT GGCAGAACGG
    ATTCGAGGCC ACGTCCTATC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAAT TTATTCCCTC AGACCAGCAG GTAATTAATG AGATGGTTCG GGAGCTAGAC
    GGCCACGTCC TGAAGTGTGT GAAAGACCAG AATGGTAATC ACGTGGTTCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TTTGCAGTTT ATTATTGATG CGTTTAAGGG GCAGGTATTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATCCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTATGT GATTCAGCAC GTATTGGAGC ATGGCCGTCC TGAGGACAAA
    AGTAAAATTG TAGCAGAAAT CCGAGGCAAT GTGCTTGTAT TGAGTCAGCA CAAATTTGCA
    AGCAACGTTG TGGAGAAGTG TGTCACTCAT GCGTCCCGCA CAGAGCGTGC TGTGCTTATC
    GACGAGGTAT GCACCATGAA TGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGCGCAAG
    ATCGTCATGC ATAAGATCCG GCCCCACATT GCGACTCTCC GCAAATACAC CTATGGCAAG
    CACATTTTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGTGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268079.1
    CDS80..3766
    Translation

    Target ORF information:

    RefSeq Version XM_022412371.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CTCTTGCCAG GTAAAAAGTT TTGGGAAACA GATGAATCCA GCAAAGATGG ACCAAAAGGA
    ATATTCCTGG GTGATCAATG GCGAGACAGT GCCTGGGGAA CATCAGATCA TTCAGTTTCC
    CAGCCAATCA TGGTGCAGAG AAGACCTGGT CAGAGTTTCC ATGTGAACAG TGAGGTCAAT
    TCTGTACTGT CCCCACGATC GGAGAGTGGG GGACTAGGCG TTAGCATGGT GGAGTATGTG
    TTGAGCTCAT CCCCGGGCGA TTCCTGTCTA AGAAAAGGAG GATTTGGCCC AAGGGATGCA
    GACAGTGATG AAAACGACAA AGGTGACAAG AAGAACAAGG GTGCGTTTGA TGGAGATAAG
    CTAGGAGATT TGAAGGAGGA GGGTGATGTG ATGGACAAGA CGAATGGTTT GCCAGTGCAG
    AATGGGATTG ATGCAGACGT CAAAGATTTT AGCCGTACCC CTGGTAATTG CCAGAACTCT
    GCTAATGAAG TGGATCTTCT GGGTCCAAAC CAGAATGGTT CTGAGGGCTT AGCCCAGCTG
    ACCAGCACCA ATGGTGCCAA GCCTGTGGAG GATTTCTCCA ACATGGAGTC CCAGAGTGTC
    CCCTTGGACC CCATGGAACA TGTGGGCATG GAGCCTCTTC AGTTTGATTA TTCAGGCACG
    CAGGTACCTG TGGACTCAGC AGCAGCAACT GTGGGACTTT TTGACTACAA TTCTCAACAA
    CAGCTGTTCC AAAGACCTAA TGCACTTGCT GTCCAGCAGT TGACAGCTGC CCAGCAGCAG
    CAGTATGCAC TGGCAGCGGC TCATCAACCT CACATCGGTG TGTTTTCAGC AGGTTTAGCG
    CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGTGCTG CTCCCCCGGG GACGGACCCC
    TACACAGCTG GACTGGCTGC AGCGGCGACA CTAGGCCCGG CTGTGGTCCC TCACCAGTAT
    TATGGAGTCA CTCCCTGGGG AGTCTACCCA GCCAGTCTCT TCCAGCAGCA AGCAGCTGCT
    GCTGCGGCAG CAACTAATTC TGCAAATCAG CAGACTACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CCCCAAATCA GAACCAGCAG
    GGACAGCAAA CAGATCCTCT GGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCTTTTGGA
    CAAGGACTGG CTGCAGGCAT GCCAGGTTAC CCGGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGCG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGCGC TCCTGTTCGG
    CTGGTAGCTC CTGCCCCAGT CATTATCAGT TCCTCGGCTG CACAAGCAGC TGTTGCGGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACCCAGCA GCCCCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAGCCTG
    GCATCCAGCT CTTTCTATGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGCCT CACCAATGGC
    AGTGGGAGAT ACATCTCTGC CGCGCCAGGC GCTGAAGCCA AGTACCGCAG CGCGAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTGTTTTCTT CTTCTCGGTT GCGATATGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTCTGG AAGATTTCCG AAATAACCGG
    TACCCCAATT TACAACTGCG GGAGATTGCT GGGCATATAA TGGAGTTTTC CCAAGACCAG
    CATGGGTCCA GATTCATTCA GCTGAAACTA GAACGAGCCA CACCAGCTGA ACGCCAGCTT
    GTCTTCAATG AAATCCTCCA GGCCGCTTAC CAGCTAATGG TGGATGTATT TGGAAATTAC
    GTCATTCAAA AGTTCTTTGA ATTTGGCAGC CTAGAACAGA AGCTGGCTTT GGCAGAACGG
    ATTCGAGGCC ACGTCCTATC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAAT TTATTCCCTC AGACCAGCAG GTAATTAATG AGATGGTTCG GGAGCTAGAC
    GGCCACGTCC TGAAGTGTGT GAAAGACCAG AATGGTAATC ACGTGGTTCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TTTGCAGTTT ATTATTGATG CGTTTAAGGG GCAGGTATTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATCCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTATGT GATTCAGCAC GTATTGGAGC ATGGCCGTCC TGAGGACAAA
    AGTAAAATTG TAGCAGAAAT CCGAGGCAAT GTGCTTGTAT TGAGTCAGCA CAAATTTGCA
    AGCAACGTTG TGGAGAAGTG TGTCACTCAT GCGTCCCGCA CAGAGCGTGC TGTGCTTATC
    GACGAGGTAT GCACCATGAA TGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGCGCAAG
    ATCGTCATGC ATAAGATCCG GCCCCACATT GCGACTCTCC GCAAATACAC CTATGGCAAG
    CACATTTTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGTGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286879
    Clone ID Related Accession (Same CDS sequence) XM_022412372.1
    Accession Version XM_022412372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3684bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTGTGTTTTC AGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGCTGTTGCG
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACCCA GCAGCCCCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAGC
    CTGGCATCCA GCTCTTTCTA TGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCTGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG CCTCACCAAT
    GGCAGTGGGA GATACATCTC TGCCGCGCCA GGCGCTGAAG CCAAGTACCG CAGCGCGAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTGTTTT CTTCTTCTCG GTTGCGATAT
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTC TGGAAGATTT CCGAAATAAC
    CGGTACCCCA ATTTACAACT GCGGGAGATT GCTGGGCATA TAATGGAGTT TTCCCAAGAC
    CAGCATGGGT CCAGATTCAT TCAGCTGAAA CTAGAACGAG CCACACCAGC TGAACGCCAG
    CTTGTCTTCA ATGAAATCCT CCAGGCCGCT TACCAGCTAA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AAAAGTTCTT TGAATTTGGC AGCCTAGAAC AGAAGCTGGC TTTGGCAGAA
    CGGATTCGAG GCCACGTCCT ATCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AATTTATTCC CTCAGACCAG CAGAATGAGA TGGTTCGGGA GCTAGACGGC
    CACGTCCTGA AGTGTGTGAA AGACCAGAAT GGTAATCACG TGGTTCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTTT GCAGTTTATT ATTGATGCGT TTAAGGGGCA GGTATTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TCCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTATGTGAT TCAGCACGTA TTGGAGCATG GCCGTCCTGA GGACAAAAGT
    AAAATTGTAG CAGAAATCCG AGGCAATGTG CTTGTATTGA GTCAGCACAA ATTTGCAAGC
    AACGTTGTGG AGAAGTGTGT CACTCATGCG TCCCGCACAG AGCGTGCTGT GCTTATCGAC
    GAGGTATGCA CCATGAATGA CGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GCGCAAGATC
    GTCATGCATA AGATCCGGCC CCACATTGCG ACTCTCCGCA AATACACCTA TGGCAAGCAC
    ATTTTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGT
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268080.1
    CDS79..3762
    Translation

    Target ORF information:

    RefSeq Version XM_022412372.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTGTGTTTTC AGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGCTGTTGCG
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACCCA GCAGCCCCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAGC
    CTGGCATCCA GCTCTTTCTA TGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCTGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG CCTCACCAAT
    GGCAGTGGGA GATACATCTC TGCCGCGCCA GGCGCTGAAG CCAAGTACCG CAGCGCGAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTGTTTT CTTCTTCTCG GTTGCGATAT
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTC TGGAAGATTT CCGAAATAAC
    CGGTACCCCA ATTTACAACT GCGGGAGATT GCTGGGCATA TAATGGAGTT TTCCCAAGAC
    CAGCATGGGT CCAGATTCAT TCAGCTGAAA CTAGAACGAG CCACACCAGC TGAACGCCAG
    CTTGTCTTCA ATGAAATCCT CCAGGCCGCT TACCAGCTAA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AAAAGTTCTT TGAATTTGGC AGCCTAGAAC AGAAGCTGGC TTTGGCAGAA
    CGGATTCGAG GCCACGTCCT ATCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AATTTATTCC CTCAGACCAG CAGAATGAGA TGGTTCGGGA GCTAGACGGC
    CACGTCCTGA AGTGTGTGAA AGACCAGAAT GGTAATCACG TGGTTCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTTT GCAGTTTATT ATTGATGCGT TTAAGGGGCA GGTATTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TCCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTATGTGAT TCAGCACGTA TTGGAGCATG GCCGTCCTGA GGACAAAAGT
    AAAATTGTAG CAGAAATCCG AGGCAATGTG CTTGTATTGA GTCAGCACAA ATTTGCAAGC
    AACGTTGTGG AGAAGTGTGT CACTCATGCG TCCCGCACAG AGCGTGCTGT GCTTATCGAC
    GAGGTATGCA CCATGAATGA CGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GCGCAAGATC
    GTCATGCATA AGATCCGGCC CCACATTGCG ACTCTCCGCA AATACACCTA TGGCAAGCAC
    ATTTTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGT
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286880
    Clone ID Related Accession (Same CDS sequence) XM_022412376.1
    Accession Version XM_022412376.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG CAGGTTTAGC GCCCGCTGCG
    TTTGTCCCCA ATCCATACAT CATCAGTGCT GCTCCCCCGG GGACGGACCC CTACACAGCT
    GGACTGGCTG CAGCGGCGAC ACTAGGCCCG GCTGTGGTCC CTCACCAGTA TTATGGAGTC
    ACTCCCTGGG GAGTCTACCC AGCCAGTCTC TTCCAGCAGC AAGCAGCTGC TGCTGCGGCA
    GCAACTAATT CTGCAAATCA GCAGACTACC CCACAGGCTC AGCAAGGGCA GCAGCAGGTT
    CTCCGTGGAG GAGCCAGCCA GCGTCCTTTG ACCCCAAATC AGAACCAGCA GGGACAGCAA
    ACAGATCCTC TGGTGGCAGC TGCAGCAGTG AATTCTGCCC TTGCTTTTGG ACAAGGACTG
    GCTGCAGGCA TGCCAGGTTA CCCGGTCTTG GCTCCTGCTG CTTACTATGA CCAAACCGGC
    GCCCTTGTGG TGAATGCAGG AGCGAGAAAT GGTCTTGGCG CTCCTGTTCG GCTGGTAGCT
    CCTGCCCCAG TCATTATCAG TTCCTCGGCT GCACAAGCAG CTGTTGCGGC GGCCGCAGCT
    TCAGCAAATG GAGCAGCCGG TGGTCTTGCT GGAACAACAA ATGGACCATT TCGCCCTTTA
    GGAACCCAGC AGCCCCAGCC CCAGCCCCAG CAGCAGCCCA GTAACAGCCT GGCATCCAGC
    TCTTTCTATG GCAACAACTC TCTGAGCAGC AATTCACAGA GCAGCTCCCT CTTCTCCCAG
    GGCTCTGCCC AGCCTGCCAA CACATCCTTG GGATTTGGAA GTAGCAGTTC TCTCGGCGCC
    ACCCTGGGAT CAGCCCTTGG AGGGTTTGGA ACAGCAGTTG CAAACTCCAA CACTGGCAGT
    GGCTCCCGCC GTGACTCCCT GACTGGCAGC AGTGACCTTT ATAAGAGGAC ATCGAGCAGC
    TTGACCCCCA TTGGACACAG TTTTTATAAC GGCCTTAGCT TTTCCTCCTC TCCTGGACCC
    GTGGGCATGC CTCTCCCTAG TCAGGGACCA GGACATTCAC AGACACCACC TCCTTCCCTC
    TCTTCACATG GATCCTCTTC AAGCTTAAAC CTGGGAGGCC TCACCAATGG CAGTGGGAGA
    TACATCTCTG CCGCGCCAGG CGCTGAAGCC AAGTACCGCA GCGCGAGCAG TGCCTCCAGC
    CTCTTCAGCC CCAGCAGCAC CCTGTTTTCT TCTTCTCGGT TGCGATATGG AATGTCTGAT
    GTCATGCCCT CTGGCAGGAG CAGGCTTCTG GAAGATTTCC GAAATAACCG GTACCCCAAT
    TTACAACTGC GGGAGATTGC TGGGCATATA ATGGAGTTTT CCCAAGACCA GCATGGGTCC
    AGATTCATTC AGCTGAAACT AGAACGAGCC ACACCAGCTG AACGCCAGCT TGTCTTCAAT
    GAAATCCTCC AGGCCGCTTA CCAGCTAATG GTGGATGTAT TTGGAAATTA CGTCATTCAA
    AAGTTCTTTG AATTTGGCAG CCTAGAACAG AAGCTGGCTT TGGCAGAACG GATTCGAGGC
    CACGTCCTAT CATTGGCACT GCAGATGTAT GGCTGCCGTG TTATCCAGAA AGCTCTTGAA
    TTTATTCCCT CAGACCAGCA GGTAATTAAT GAGATGGTTC GGGAGCTAGA CGGCCACGTC
    CTGAAGTGTG TGAAAGACCA GAATGGTAAT CACGTGGTTC AGAAGTGCAT CGAATGTGTG
    CAGCCCCAGT CTTTGCAGTT TATTATTGAT GCGTTTAAGG GGCAGGTATT TGCTTTGTCC
    ACGCATCCTT ATGGCTGCCG AGTGATTCAG AGGATCCTGG AGCACTGTCT CCCTGACCAG
    ACACTCCCTA TTTTAGAGGA GCTTCACCAG CACACAGAGC AACTTGTACA GGATCAGTAT
    GGAAATTATG TGATTCAGCA CGTATTGGAG CATGGCCGTC CTGAGGACAA AAGTAAAATT
    GTAGCAGAAA TCCGAGGCAA TGTGCTTGTA TTGAGTCAGC ACAAATTTGC AAGCAACGTT
    GTGGAGAAGT GTGTCACTCA TGCGTCCCGC ACAGAGCGTG CTGTGCTTAT CGACGAGGTA
    TGCACCATGA ATGACGGTCC CCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AACTACGTGG TCCAGAAGAT GATCGATGTG GCAGAGCCAG CCCAGCGCAA GATCGTCATG
    CATAAGATCC GGCCCCACAT TGCGACTCTC CGCAAATACA CCTATGGCAA GCACATTTTG
    GCCAAGCTGG AGAAATACTA CATGAAGAAT GGCGTTGACT TAGGGCCCAT CTGTGGCCCC
    CCTAATGGTA TTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268084.1
    CDS78..3755
    Translation

    Target ORF information:

    RefSeq Version XM_022412376.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412376.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG CAGGTTTAGC GCCCGCTGCG
    TTTGTCCCCA ATCCATACAT CATCAGTGCT GCTCCCCCGG GGACGGACCC CTACACAGCT
    GGACTGGCTG CAGCGGCGAC ACTAGGCCCG GCTGTGGTCC CTCACCAGTA TTATGGAGTC
    ACTCCCTGGG GAGTCTACCC AGCCAGTCTC TTCCAGCAGC AAGCAGCTGC TGCTGCGGCA
    GCAACTAATT CTGCAAATCA GCAGACTACC CCACAGGCTC AGCAAGGGCA GCAGCAGGTT
    CTCCGTGGAG GAGCCAGCCA GCGTCCTTTG ACCCCAAATC AGAACCAGCA GGGACAGCAA
    ACAGATCCTC TGGTGGCAGC TGCAGCAGTG AATTCTGCCC TTGCTTTTGG ACAAGGACTG
    GCTGCAGGCA TGCCAGGTTA CCCGGTCTTG GCTCCTGCTG CTTACTATGA CCAAACCGGC
    GCCCTTGTGG TGAATGCAGG AGCGAGAAAT GGTCTTGGCG CTCCTGTTCG GCTGGTAGCT
    CCTGCCCCAG TCATTATCAG TTCCTCGGCT GCACAAGCAG CTGTTGCGGC GGCCGCAGCT
    TCAGCAAATG GAGCAGCCGG TGGTCTTGCT GGAACAACAA ATGGACCATT TCGCCCTTTA
    GGAACCCAGC AGCCCCAGCC CCAGCCCCAG CAGCAGCCCA GTAACAGCCT GGCATCCAGC
    TCTTTCTATG GCAACAACTC TCTGAGCAGC AATTCACAGA GCAGCTCCCT CTTCTCCCAG
    GGCTCTGCCC AGCCTGCCAA CACATCCTTG GGATTTGGAA GTAGCAGTTC TCTCGGCGCC
    ACCCTGGGAT CAGCCCTTGG AGGGTTTGGA ACAGCAGTTG CAAACTCCAA CACTGGCAGT
    GGCTCCCGCC GTGACTCCCT GACTGGCAGC AGTGACCTTT ATAAGAGGAC ATCGAGCAGC
    TTGACCCCCA TTGGACACAG TTTTTATAAC GGCCTTAGCT TTTCCTCCTC TCCTGGACCC
    GTGGGCATGC CTCTCCCTAG TCAGGGACCA GGACATTCAC AGACACCACC TCCTTCCCTC
    TCTTCACATG GATCCTCTTC AAGCTTAAAC CTGGGAGGCC TCACCAATGG CAGTGGGAGA
    TACATCTCTG CCGCGCCAGG CGCTGAAGCC AAGTACCGCA GCGCGAGCAG TGCCTCCAGC
    CTCTTCAGCC CCAGCAGCAC CCTGTTTTCT TCTTCTCGGT TGCGATATGG AATGTCTGAT
    GTCATGCCCT CTGGCAGGAG CAGGCTTCTG GAAGATTTCC GAAATAACCG GTACCCCAAT
    TTACAACTGC GGGAGATTGC TGGGCATATA ATGGAGTTTT CCCAAGACCA GCATGGGTCC
    AGATTCATTC AGCTGAAACT AGAACGAGCC ACACCAGCTG AACGCCAGCT TGTCTTCAAT
    GAAATCCTCC AGGCCGCTTA CCAGCTAATG GTGGATGTAT TTGGAAATTA CGTCATTCAA
    AAGTTCTTTG AATTTGGCAG CCTAGAACAG AAGCTGGCTT TGGCAGAACG GATTCGAGGC
    CACGTCCTAT CATTGGCACT GCAGATGTAT GGCTGCCGTG TTATCCAGAA AGCTCTTGAA
    TTTATTCCCT CAGACCAGCA GGTAATTAAT GAGATGGTTC GGGAGCTAGA CGGCCACGTC
    CTGAAGTGTG TGAAAGACCA GAATGGTAAT CACGTGGTTC AGAAGTGCAT CGAATGTGTG
    CAGCCCCAGT CTTTGCAGTT TATTATTGAT GCGTTTAAGG GGCAGGTATT TGCTTTGTCC
    ACGCATCCTT ATGGCTGCCG AGTGATTCAG AGGATCCTGG AGCACTGTCT CCCTGACCAG
    ACACTCCCTA TTTTAGAGGA GCTTCACCAG CACACAGAGC AACTTGTACA GGATCAGTAT
    GGAAATTATG TGATTCAGCA CGTATTGGAG CATGGCCGTC CTGAGGACAA AAGTAAAATT
    GTAGCAGAAA TCCGAGGCAA TGTGCTTGTA TTGAGTCAGC ACAAATTTGC AAGCAACGTT
    GTGGAGAAGT GTGTCACTCA TGCGTCCCGC ACAGAGCGTG CTGTGCTTAT CGACGAGGTA
    TGCACCATGA ATGACGGTCC CCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AACTACGTGG TCCAGAAGAT GATCGATGTG GCAGAGCCAG CCCAGCGCAA GATCGTCATG
    CATAAGATCC GGCCCCACAT TGCGACTCTC CGCAAATACA CCTATGGCAA GCACATTTTG
    GCCAAGCTGG AGAAATACTA CATGAAGAAT GGCGTTGACT TAGGGCCCAT CTGTGGCCCC
    CCTAATGGTA TTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286881
    Clone ID Related Accession (Same CDS sequence) XM_022412380.1
    Accession Version XM_022412380.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTTTAGCGCC CGCTGCGTTT
    GTCCCCAATC CATACATCAT CAGTGCTGCT CCCCCGGGGA CGGACCCCTA CACAGCTGGA
    CTGGCTGCAG CGGCGACACT AGGCCCGGCT GTGGTCCCTC ACCAGTATTA TGGAGTCACT
    CCCTGGGGAG TCTACCCAGC CAGTCTCTTC CAGCAGCAAG CAGCTGCTGC TGCGGCAGCA
    ACTAATTCTG CAAATCAGCA GACTACCCCA CAGGCTCAGC AAGGGCAGCA GCAGGTTCTC
    CGTGGAGGAG CCAGCCAGCG TCCTTTGACC CCAAATCAGA ACCAGCAGGG ACAGCAAACA
    GATCCTCTGG TGGCAGCTGC AGCAGTGAAT TCTGCCCTTG CTTTTGGACA AGGACTGGCT
    GCAGGCATGC CAGGTTACCC GGTCTTGGCT CCTGCTGCTT ACTATGACCA AACCGGCGCC
    CTTGTGGTGA ATGCAGGAGC GAGAAATGGT CTTGGCGCTC CTGTTCGGCT GGTAGCTCCT
    GCCCCAGTCA TTATCAGTTC CTCGGCTGCA CAAGCAGCTG TTGCGGCGGC CGCAGCTTCA
    GCAAATGGAG CAGCCGGTGG TCTTGCTGGA ACAACAAATG GACCATTTCG CCCTTTAGGA
    ACCCAGCAGC CCCAGCCCCA GCCCCAGCAG CAGCCCAGTA ACAGCCTGGC ATCCAGCTCT
    TTCTATGGCA ACAACTCTCT GAGCAGCAAT TCACAGAGCA GCTCCCTCTT CTCCCAGGGC
    TCTGCCCAGC CTGCCAACAC ATCCTTGGGA TTTGGAAGTA GCAGTTCTCT CGGCGCCACC
    CTGGGATCAG CCCTTGGAGG GTTTGGAACA GCAGTTGCAA ACTCCAACAC TGGCAGTGGC
    TCCCGCCGTG ACTCCCTGAC TGGCAGCAGT GACCTTTATA AGAGGACATC GAGCAGCTTG
    ACCCCCATTG GACACAGTTT TTATAACGGC CTTAGCTTTT CCTCCTCTCC TGGACCCGTG
    GGCATGCCTC TCCCTAGTCA GGGACCAGGA CATTCACAGA CACCACCTCC TTCCCTCTCT
    TCACATGGAT CCTCTTCAAG CTTAAACCTG GGAGGCCTCA CCAATGGCAG TGGGAGATAC
    ATCTCTGCCG CGCCAGGCGC TGAAGCCAAG TACCGCAGCG CGAGCAGTGC CTCCAGCCTC
    TTCAGCCCCA GCAGCACCCT GTTTTCTTCT TCTCGGTTGC GATATGGAAT GTCTGATGTC
    ATGCCCTCTG GCAGGAGCAG GCTTCTGGAA GATTTCCGAA ATAACCGGTA CCCCAATTTA
    CAACTGCGGG AGATTGCTGG GCATATAATG GAGTTTTCCC AAGACCAGCA TGGGTCCAGA
    TTCATTCAGC TGAAACTAGA ACGAGCCACA CCAGCTGAAC GCCAGCTTGT CTTCAATGAA
    ATCCTCCAGG CCGCTTACCA GCTAATGGTG GATGTATTTG GAAATTACGT CATTCAAAAG
    TTCTTTGAAT TTGGCAGCCT AGAACAGAAG CTGGCTTTGG CAGAACGGAT TCGAGGCCAC
    GTCCTATCAT TGGCACTGCA GATGTATGGC TGCCGTGTTA TCCAGAAAGC TCTTGAATTT
    ATTCCCTCAG ACCAGCAGGT AATTAATGAG ATGGTTCGGG AGCTAGACGG CCACGTCCTG
    AAGTGTGTGA AAGACCAGAA TGGTAATCAC GTGGTTCAGA AGTGCATCGA ATGTGTGCAG
    CCCCAGTCTT TGCAGTTTAT TATTGATGCG TTTAAGGGGC AGGTATTTGC TTTGTCCACG
    CATCCTTATG GCTGCCGAGT GATTCAGAGG ATCCTGGAGC ACTGTCTCCC TGACCAGACA
    CTCCCTATTT TAGAGGAGCT TCACCAGCAC ACAGAGCAAC TTGTACAGGA TCAGTATGGA
    AATTATGTGA TTCAGCACGT ATTGGAGCAT GGCCGTCCTG AGGACAAAAG TAAAATTGTA
    GCAGAAATCC GAGGCAATGT GCTTGTATTG AGTCAGCACA AATTTGCAAG CAACGTTGTG
    GAGAAGTGTG TCACTCATGC GTCCCGCACA GAGCGTGCTG TGCTTATCGA CGAGGTATGC
    ACCATGAATG ACGGTCCCCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAC
    TACGTGGTCC AGAAGATGAT CGATGTGGCA GAGCCAGCCC AGCGCAAGAT CGTCATGCAT
    AAGATCCGGC CCCACATTGC GACTCTCCGC AAATACACCT ATGGCAAGCA CATTTTGGCC
    AAGCTGGAGA AATACTACAT GAAGAATGGC GTTGACTTAG GGCCCATCTG TGGCCCCCCT
    AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268088.1
    CDS78..3752
    Translation

    Target ORF information:

    RefSeq Version XM_022412380.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTTTAGCGCC CGCTGCGTTT
    GTCCCCAATC CATACATCAT CAGTGCTGCT CCCCCGGGGA CGGACCCCTA CACAGCTGGA
    CTGGCTGCAG CGGCGACACT AGGCCCGGCT GTGGTCCCTC ACCAGTATTA TGGAGTCACT
    CCCTGGGGAG TCTACCCAGC CAGTCTCTTC CAGCAGCAAG CAGCTGCTGC TGCGGCAGCA
    ACTAATTCTG CAAATCAGCA GACTACCCCA CAGGCTCAGC AAGGGCAGCA GCAGGTTCTC
    CGTGGAGGAG CCAGCCAGCG TCCTTTGACC CCAAATCAGA ACCAGCAGGG ACAGCAAACA
    GATCCTCTGG TGGCAGCTGC AGCAGTGAAT TCTGCCCTTG CTTTTGGACA AGGACTGGCT
    GCAGGCATGC CAGGTTACCC GGTCTTGGCT CCTGCTGCTT ACTATGACCA AACCGGCGCC
    CTTGTGGTGA ATGCAGGAGC GAGAAATGGT CTTGGCGCTC CTGTTCGGCT GGTAGCTCCT
    GCCCCAGTCA TTATCAGTTC CTCGGCTGCA CAAGCAGCTG TTGCGGCGGC CGCAGCTTCA
    GCAAATGGAG CAGCCGGTGG TCTTGCTGGA ACAACAAATG GACCATTTCG CCCTTTAGGA
    ACCCAGCAGC CCCAGCCCCA GCCCCAGCAG CAGCCCAGTA ACAGCCTGGC ATCCAGCTCT
    TTCTATGGCA ACAACTCTCT GAGCAGCAAT TCACAGAGCA GCTCCCTCTT CTCCCAGGGC
    TCTGCCCAGC CTGCCAACAC ATCCTTGGGA TTTGGAAGTA GCAGTTCTCT CGGCGCCACC
    CTGGGATCAG CCCTTGGAGG GTTTGGAACA GCAGTTGCAA ACTCCAACAC TGGCAGTGGC
    TCCCGCCGTG ACTCCCTGAC TGGCAGCAGT GACCTTTATA AGAGGACATC GAGCAGCTTG
    ACCCCCATTG GACACAGTTT TTATAACGGC CTTAGCTTTT CCTCCTCTCC TGGACCCGTG
    GGCATGCCTC TCCCTAGTCA GGGACCAGGA CATTCACAGA CACCACCTCC TTCCCTCTCT
    TCACATGGAT CCTCTTCAAG CTTAAACCTG GGAGGCCTCA CCAATGGCAG TGGGAGATAC
    ATCTCTGCCG CGCCAGGCGC TGAAGCCAAG TACCGCAGCG CGAGCAGTGC CTCCAGCCTC
    TTCAGCCCCA GCAGCACCCT GTTTTCTTCT TCTCGGTTGC GATATGGAAT GTCTGATGTC
    ATGCCCTCTG GCAGGAGCAG GCTTCTGGAA GATTTCCGAA ATAACCGGTA CCCCAATTTA
    CAACTGCGGG AGATTGCTGG GCATATAATG GAGTTTTCCC AAGACCAGCA TGGGTCCAGA
    TTCATTCAGC TGAAACTAGA ACGAGCCACA CCAGCTGAAC GCCAGCTTGT CTTCAATGAA
    ATCCTCCAGG CCGCTTACCA GCTAATGGTG GATGTATTTG GAAATTACGT CATTCAAAAG
    TTCTTTGAAT TTGGCAGCCT AGAACAGAAG CTGGCTTTGG CAGAACGGAT TCGAGGCCAC
    GTCCTATCAT TGGCACTGCA GATGTATGGC TGCCGTGTTA TCCAGAAAGC TCTTGAATTT
    ATTCCCTCAG ACCAGCAGGT AATTAATGAG ATGGTTCGGG AGCTAGACGG CCACGTCCTG
    AAGTGTGTGA AAGACCAGAA TGGTAATCAC GTGGTTCAGA AGTGCATCGA ATGTGTGCAG
    CCCCAGTCTT TGCAGTTTAT TATTGATGCG TTTAAGGGGC AGGTATTTGC TTTGTCCACG
    CATCCTTATG GCTGCCGAGT GATTCAGAGG ATCCTGGAGC ACTGTCTCCC TGACCAGACA
    CTCCCTATTT TAGAGGAGCT TCACCAGCAC ACAGAGCAAC TTGTACAGGA TCAGTATGGA
    AATTATGTGA TTCAGCACGT ATTGGAGCAT GGCCGTCCTG AGGACAAAAG TAAAATTGTA
    GCAGAAATCC GAGGCAATGT GCTTGTATTG AGTCAGCACA AATTTGCAAG CAACGTTGTG
    GAGAAGTGTG TCACTCATGC GTCCCGCACA GAGCGTGCTG TGCTTATCGA CGAGGTATGC
    ACCATGAATG ACGGTCCCCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAC
    TACGTGGTCC AGAAGATGAT CGATGTGGCA GAGCCAGCCC AGCGCAAGAT CGTCATGCAT
    AAGATCCGGC CCCACATTGC GACTCTCCGC AAATACACCT ATGGCAAGCA CATTTTGGCC
    AAGCTGGAGA AATACTACAT GAAGAATGGC GTTGACTTAG GGCCCATCTG TGGCCCCCCT
    AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286882
    Clone ID Related Accession (Same CDS sequence) XM_022412382.1
    Accession Version XM_022412382.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTTTAGCGCC CGCTGCGTTT
    GTCCCCAATC CATACATCAT CAGTGCTGCT CCCCCGGGGA CGGACCCCTA CACAGCTGGA
    CTGGCTGCAG CGGCGACACT AGGCCCGGCT GTGGTCCCTC ACCAGTATTA TGGAGTCACT
    CCCTGGGGAG TCTACCCAGC CAGTCTCTTC CAGCAGCAAG CAGCTGCTGC TGCGGCAGCA
    ACTAATTCTG CAAATCAGCA GACTACCCCA CAGGCTCAGC AAGGGCAGCA GCAGGTTCTC
    CGTGGAGGAG CCAGCCAGCG TCCTTTGACC CCAAATCAGA ACCAGCAGGG ACAGCAAACA
    GATCCTCTGG TGGCAGCTGC AGCAGTGAAT TCTGCCCTTG CTTTTGGACA AGGACTGGCT
    GCAGGCATGC CAGGTTACCC GGTCTTGGCT CCTGCTGCTT ACTATGACCA AACCGGCGCC
    CTTGTGGTGA ATGCAGGAGC GAGAAATGGT CTTGGCGCTC CTGTTCGGCT GGTAGCTCCT
    GCCCCAGTCA TTATCAGTTC CTCGGCTGCA CAAGCAGCTG TTGCGGCGGC CGCAGCTTCA
    GCAAATGGAG CAGCCGGTGG TCTTGCTGGA ACAACAAATG GACCATTTCG CCCTTTAGGA
    ACCCAGCAGC CCCAGCCCCA GCCCCAGCAG CAGCCCAGTA ACAGCCTGGC ATCCAGCTCT
    TTCTATGGCA ACAACTCTCT GAGCAGCAAT TCACAGAGCA GCTCCCTCTT CTCCCAGGGC
    TCTGCCCAGC CTGCCAACAC ATCCTTGGGA TTTGGAAGTA GCAGTTCTCT CGGCGCCACC
    CTGGGATCAG CCCTTGGAGG GTTTGGAACA GCAGTTGCAA ACTCCAACAC TGGCAGTGGC
    TCCCGCCGTG ACTCCCTGAC TGGCAGCAGT GACCTTTATA AGAGGACATC GAGCAGCTTG
    ACCCCCATTG GACACAGTTT TTATAACGGC CTTAGCTTTT CCTCCTCTCC TGGACCCGTG
    GGCATGCCTC TCCCTAGTCA GGGACCAGGA CATTCACAGA CACCACCTCC TTCCCTCTCT
    TCACATGGAT CCTCTTCAAG CTTAAACCTG GGAGGCCTCA CCAATGGCAG TGGGAGATAC
    ATCTCTGCCG CGCCAGGCGC TGAAGCCAAG TACCGCAGCG CGAGCAGTGC CTCCAGCCTC
    TTCAGCCCCA GCAGCACCCT GTTTTCTTCT TCTCGGTTGC GATATGGAAT GTCTGATGTC
    ATGCCCTCTG GCAGGAGCAG GCTTCTGGAA GATTTCCGAA ATAACCGGTA CCCCAATTTA
    CAACTGCGGG AGATTGCTGG GCATATAATG GAGTTTTCCC AAGACCAGCA TGGGTCCAGA
    TTCATTCAGC TGAAACTAGA ACGAGCCACA CCAGCTGAAC GCCAGCTTGT CTTCAATGAA
    ATCCTCCAGG CCGCTTACCA GCTAATGGTG GATGTATTTG GAAATTACGT CATTCAAAAG
    TTCTTTGAAT TTGGCAGCCT AGAACAGAAG CTGGCTTTGG CAGAACGGAT TCGAGGCCAC
    GTCCTATCAT TGGCACTGCA GATGTATGGC TGCCGTGTTA TCCAGAAAGC TCTTGAATTT
    ATTCCCTCAG ACCAGCAGAA TGAGATGGTT CGGGAGCTAG ACGGCCACGT CCTGAAGTGT
    GTGAAAGACC AGAATGGTAA TCACGTGGTT CAGAAGTGCA TCGAATGTGT GCAGCCCCAG
    TCTTTGCAGT TTATTATTGA TGCGTTTAAG GGGCAGGTAT TTGCTTTGTC CACGCATCCT
    TATGGCTGCC GAGTGATTCA GAGGATCCTG GAGCACTGTC TCCCTGACCA GACACTCCCT
    ATTTTAGAGG AGCTTCACCA GCACACAGAG CAACTTGTAC AGGATCAGTA TGGAAATTAT
    GTGATTCAGC ACGTATTGGA GCATGGCCGT CCTGAGGACA AAAGTAAAAT TGTAGCAGAA
    ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG CACAAATTTG CAAGCAACGT TGTGGAGAAG
    TGTGTCACTC ATGCGTCCCG CACAGAGCGT GCTGTGCTTA TCGACGAGGT ATGCACCATG
    AATGACGGTC CCCACAGTGC CTTATACACC ATGATGAAGG ACCAGTATGC CAACTACGTG
    GTCCAGAAGA TGATCGATGT GGCAGAGCCA GCCCAGCGCA AGATCGTCAT GCATAAGATC
    CGGCCCCACA TTGCGACTCT CCGCAAATAC ACCTATGGCA AGCACATTTT GGCCAAGCTG
    GAGAAATACT ACATGAAGAA TGGCGTTGAC TTAGGGCCCA TCTGTGGCCC CCCTAATGGT
    ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268090.1
    CDS78..3746
    Translation

    Target ORF information:

    RefSeq Version XM_022412382.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTTTAGCGCC CGCTGCGTTT
    GTCCCCAATC CATACATCAT CAGTGCTGCT CCCCCGGGGA CGGACCCCTA CACAGCTGGA
    CTGGCTGCAG CGGCGACACT AGGCCCGGCT GTGGTCCCTC ACCAGTATTA TGGAGTCACT
    CCCTGGGGAG TCTACCCAGC CAGTCTCTTC CAGCAGCAAG CAGCTGCTGC TGCGGCAGCA
    ACTAATTCTG CAAATCAGCA GACTACCCCA CAGGCTCAGC AAGGGCAGCA GCAGGTTCTC
    CGTGGAGGAG CCAGCCAGCG TCCTTTGACC CCAAATCAGA ACCAGCAGGG ACAGCAAACA
    GATCCTCTGG TGGCAGCTGC AGCAGTGAAT TCTGCCCTTG CTTTTGGACA AGGACTGGCT
    GCAGGCATGC CAGGTTACCC GGTCTTGGCT CCTGCTGCTT ACTATGACCA AACCGGCGCC
    CTTGTGGTGA ATGCAGGAGC GAGAAATGGT CTTGGCGCTC CTGTTCGGCT GGTAGCTCCT
    GCCCCAGTCA TTATCAGTTC CTCGGCTGCA CAAGCAGCTG TTGCGGCGGC CGCAGCTTCA
    GCAAATGGAG CAGCCGGTGG TCTTGCTGGA ACAACAAATG GACCATTTCG CCCTTTAGGA
    ACCCAGCAGC CCCAGCCCCA GCCCCAGCAG CAGCCCAGTA ACAGCCTGGC ATCCAGCTCT
    TTCTATGGCA ACAACTCTCT GAGCAGCAAT TCACAGAGCA GCTCCCTCTT CTCCCAGGGC
    TCTGCCCAGC CTGCCAACAC ATCCTTGGGA TTTGGAAGTA GCAGTTCTCT CGGCGCCACC
    CTGGGATCAG CCCTTGGAGG GTTTGGAACA GCAGTTGCAA ACTCCAACAC TGGCAGTGGC
    TCCCGCCGTG ACTCCCTGAC TGGCAGCAGT GACCTTTATA AGAGGACATC GAGCAGCTTG
    ACCCCCATTG GACACAGTTT TTATAACGGC CTTAGCTTTT CCTCCTCTCC TGGACCCGTG
    GGCATGCCTC TCCCTAGTCA GGGACCAGGA CATTCACAGA CACCACCTCC TTCCCTCTCT
    TCACATGGAT CCTCTTCAAG CTTAAACCTG GGAGGCCTCA CCAATGGCAG TGGGAGATAC
    ATCTCTGCCG CGCCAGGCGC TGAAGCCAAG TACCGCAGCG CGAGCAGTGC CTCCAGCCTC
    TTCAGCCCCA GCAGCACCCT GTTTTCTTCT TCTCGGTTGC GATATGGAAT GTCTGATGTC
    ATGCCCTCTG GCAGGAGCAG GCTTCTGGAA GATTTCCGAA ATAACCGGTA CCCCAATTTA
    CAACTGCGGG AGATTGCTGG GCATATAATG GAGTTTTCCC AAGACCAGCA TGGGTCCAGA
    TTCATTCAGC TGAAACTAGA ACGAGCCACA CCAGCTGAAC GCCAGCTTGT CTTCAATGAA
    ATCCTCCAGG CCGCTTACCA GCTAATGGTG GATGTATTTG GAAATTACGT CATTCAAAAG
    TTCTTTGAAT TTGGCAGCCT AGAACAGAAG CTGGCTTTGG CAGAACGGAT TCGAGGCCAC
    GTCCTATCAT TGGCACTGCA GATGTATGGC TGCCGTGTTA TCCAGAAAGC TCTTGAATTT
    ATTCCCTCAG ACCAGCAGAA TGAGATGGTT CGGGAGCTAG ACGGCCACGT CCTGAAGTGT
    GTGAAAGACC AGAATGGTAA TCACGTGGTT CAGAAGTGCA TCGAATGTGT GCAGCCCCAG
    TCTTTGCAGT TTATTATTGA TGCGTTTAAG GGGCAGGTAT TTGCTTTGTC CACGCATCCT
    TATGGCTGCC GAGTGATTCA GAGGATCCTG GAGCACTGTC TCCCTGACCA GACACTCCCT
    ATTTTAGAGG AGCTTCACCA GCACACAGAG CAACTTGTAC AGGATCAGTA TGGAAATTAT
    GTGATTCAGC ACGTATTGGA GCATGGCCGT CCTGAGGACA AAAGTAAAAT TGTAGCAGAA
    ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG CACAAATTTG CAAGCAACGT TGTGGAGAAG
    TGTGTCACTC ATGCGTCCCG CACAGAGCGT GCTGTGCTTA TCGACGAGGT ATGCACCATG
    AATGACGGTC CCCACAGTGC CTTATACACC ATGATGAAGG ACCAGTATGC CAACTACGTG
    GTCCAGAAGA TGATCGATGT GGCAGAGCCA GCCCAGCGCA AGATCGTCAT GCATAAGATC
    CGGCCCCACA TTGCGACTCT CCGCAAATAC ACCTATGGCA AGCACATTTT GGCCAAGCTG
    GAGAAATACT ACATGAAGAA TGGCGTTGAC TTAGGGCCCA TCTGTGGCCC CCCTAATGGT
    ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286883
    Clone ID Related Accession (Same CDS sequence) XM_022412383.1
    Accession Version XM_022412383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTGTGTTTTC AGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGGAACCCAG
    CAGCCCCAGC CCCAGCCCCA GCAGCAGCCC AGTAACAGCC TGGCATCCAG CTCTTTCTAT
    GGCAACAACT CTCTGAGCAG CAATTCACAG AGCAGCTCCC TCTTCTCCCA GGGCTCTGCC
    CAGCCTGCCA ACACATCCTT GGGATTTGGA AGTAGCAGTT CTCTCGGCGC CACCCTGGGA
    TCAGCCCTTG GAGGGTTTGG AACAGCAGTT GCAAACTCCA ACACTGGCAG TGGCTCCCGC
    CGTGACTCCC TGACTGGCAG CAGTGACCTT TATAAGAGGA CATCGAGCAG CTTGACCCCC
    ATTGGACACA GTTTTTATAA CGGCCTTAGC TTTTCCTCCT CTCCTGGACC CGTGGGCATG
    CCTCTCCCTA GTCAGGGACC AGGACATTCA CAGACACCAC CTCCTTCCCT CTCTTCACAT
    GGATCCTCTT CAAGCTTAAA CCTGGGAGGC CTCACCAATG GCAGTGGGAG ATACATCTCT
    GCCGCGCCAG GCGCTGAAGC CAAGTACCGC AGCGCGAGCA GTGCCTCCAG CCTCTTCAGC
    CCCAGCAGCA CCCTGTTTTC TTCTTCTCGG TTGCGATATG GAATGTCTGA TGTCATGCCC
    TCTGGCAGGA GCAGGCTTCT GGAAGATTTC CGAAATAACC GGTACCCCAA TTTACAACTG
    CGGGAGATTG CTGGGCATAT AATGGAGTTT TCCCAAGACC AGCATGGGTC CAGATTCATT
    CAGCTGAAAC TAGAACGAGC CACACCAGCT GAACGCCAGC TTGTCTTCAA TGAAATCCTC
    CAGGCCGCTT ACCAGCTAAT GGTGGATGTA TTTGGAAATT ACGTCATTCA AAAGTTCTTT
    GAATTTGGCA GCCTAGAACA GAAGCTGGCT TTGGCAGAAC GGATTCGAGG CCACGTCCTA
    TCATTGGCAC TGCAGATGTA TGGCTGCCGT GTTATCCAGA AAGCTCTTGA ATTTATTCCC
    TCAGACCAGC AGGTAATTAA TGAGATGGTT CGGGAGCTAG ACGGCCACGT CCTGAAGTGT
    GTGAAAGACC AGAATGGTAA TCACGTGGTT CAGAAGTGCA TCGAATGTGT GCAGCCCCAG
    TCTTTGCAGT TTATTATTGA TGCGTTTAAG GGGCAGGTAT TTGCTTTGTC CACGCATCCT
    TATGGCTGCC GAGTGATTCA GAGGATCCTG GAGCACTGTC TCCCTGACCA GACACTCCCT
    ATTTTAGAGG AGCTTCACCA GCACACAGAG CAACTTGTAC AGGATCAGTA TGGAAATTAT
    GTGATTCAGC ACGTATTGGA GCATGGCCGT CCTGAGGACA AAAGTAAAAT TGTAGCAGAA
    ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG CACAAATTTG CAAGCAACGT TGTGGAGAAG
    TGTGTCACTC ATGCGTCCCG CACAGAGCGT GCTGTGCTTA TCGACGAGGT ATGCACCATG
    AATGACGGTC CCCACAGTGC CTTATACACC ATGATGAAGG ACCAGTATGC CAACTACGTG
    GTCCAGAAGA TGATCGATGT GGCAGAGCCA GCCCAGCGCA AGATCGTCAT GCATAAGATC
    CGGCCCCACA TTGCGACTCT CCGCAAATAC ACCTATGGCA AGCACATTTT GGCCAAGCTG
    GAGAAATACT ACATGAAGAA TGGCGTTGAC TTAGGGCCCA TCTGTGGCCC CCCTAATGGT
    ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268091.1
    CDS79..3687
    Translation

    Target ORF information:

    RefSeq Version XM_022412383.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTGTGTTTTC AGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGGAACCCAG
    CAGCCCCAGC CCCAGCCCCA GCAGCAGCCC AGTAACAGCC TGGCATCCAG CTCTTTCTAT
    GGCAACAACT CTCTGAGCAG CAATTCACAG AGCAGCTCCC TCTTCTCCCA GGGCTCTGCC
    CAGCCTGCCA ACACATCCTT GGGATTTGGA AGTAGCAGTT CTCTCGGCGC CACCCTGGGA
    TCAGCCCTTG GAGGGTTTGG AACAGCAGTT GCAAACTCCA ACACTGGCAG TGGCTCCCGC
    CGTGACTCCC TGACTGGCAG CAGTGACCTT TATAAGAGGA CATCGAGCAG CTTGACCCCC
    ATTGGACACA GTTTTTATAA CGGCCTTAGC TTTTCCTCCT CTCCTGGACC CGTGGGCATG
    CCTCTCCCTA GTCAGGGACC AGGACATTCA CAGACACCAC CTCCTTCCCT CTCTTCACAT
    GGATCCTCTT CAAGCTTAAA CCTGGGAGGC CTCACCAATG GCAGTGGGAG ATACATCTCT
    GCCGCGCCAG GCGCTGAAGC CAAGTACCGC AGCGCGAGCA GTGCCTCCAG CCTCTTCAGC
    CCCAGCAGCA CCCTGTTTTC TTCTTCTCGG TTGCGATATG GAATGTCTGA TGTCATGCCC
    TCTGGCAGGA GCAGGCTTCT GGAAGATTTC CGAAATAACC GGTACCCCAA TTTACAACTG
    CGGGAGATTG CTGGGCATAT AATGGAGTTT TCCCAAGACC AGCATGGGTC CAGATTCATT
    CAGCTGAAAC TAGAACGAGC CACACCAGCT GAACGCCAGC TTGTCTTCAA TGAAATCCTC
    CAGGCCGCTT ACCAGCTAAT GGTGGATGTA TTTGGAAATT ACGTCATTCA AAAGTTCTTT
    GAATTTGGCA GCCTAGAACA GAAGCTGGCT TTGGCAGAAC GGATTCGAGG CCACGTCCTA
    TCATTGGCAC TGCAGATGTA TGGCTGCCGT GTTATCCAGA AAGCTCTTGA ATTTATTCCC
    TCAGACCAGC AGGTAATTAA TGAGATGGTT CGGGAGCTAG ACGGCCACGT CCTGAAGTGT
    GTGAAAGACC AGAATGGTAA TCACGTGGTT CAGAAGTGCA TCGAATGTGT GCAGCCCCAG
    TCTTTGCAGT TTATTATTGA TGCGTTTAAG GGGCAGGTAT TTGCTTTGTC CACGCATCCT
    TATGGCTGCC GAGTGATTCA GAGGATCCTG GAGCACTGTC TCCCTGACCA GACACTCCCT
    ATTTTAGAGG AGCTTCACCA GCACACAGAG CAACTTGTAC AGGATCAGTA TGGAAATTAT
    GTGATTCAGC ACGTATTGGA GCATGGCCGT CCTGAGGACA AAAGTAAAAT TGTAGCAGAA
    ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG CACAAATTTG CAAGCAACGT TGTGGAGAAG
    TGTGTCACTC ATGCGTCCCG CACAGAGCGT GCTGTGCTTA TCGACGAGGT ATGCACCATG
    AATGACGGTC CCCACAGTGC CTTATACACC ATGATGAAGG ACCAGTATGC CAACTACGTG
    GTCCAGAAGA TGATCGATGT GGCAGAGCCA GCCCAGCGCA AGATCGTCAT GCATAAGATC
    CGGCCCCACA TTGCGACTCT CCGCAAATAC ACCTATGGCA AGCACATTTT GGCCAAGCTG
    GAGAAATACT ACATGAAGAA TGGCGTTGAC TTAGGGCCCA TCTGTGGCCC CCCTAATGGT
    ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286884
    Clone ID Related Accession (Same CDS sequence) XM_022412387.1
    Accession Version XM_022412387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GCCCGGCTGT GGTCCCTCAC
    CAGTATTATG GAGTCACTCC CTGGGGAGTC TACCCAGCCA GTCTCTTCCA GCAGCAAGCA
    GCTGCTGCTG CGGCAGCAAC TAATTCTGCA AATCAGCAGA CTACCCCACA GGCTCAGCAA
    GGGCAGCAGC AGGTTCTCCG TGGAGGAGCC AGCCAGCGTC CTTTGACCCC AAATCAGAAC
    CAGCAGGGAC AGCAAACAGA TCCTCTGGTG GCAGCTGCAG CAGTGAATTC TGCCCTTGCT
    TTTGGACAAG GACTGGCTGC AGGCATGCCA GGTTACCCGG TCTTGGCTCC TGCTGCTTAC
    TATGACCAAA CCGGCGCCCT TGTGGTGAAT GCAGGAGCGA GAAATGGTCT TGGCGCTCCT
    GTTCGGCTGG TAGCTCCTGC CCCAGTCATT ATCAGTTCCT CGGCTGCACA AGCAGCTGTT
    GCGGCGGCCG CAGCTTCAGC AAATGGAGCA GCCGGTGGTC TTGCTGGAAC AACAAATGGA
    CCATTTCGCC CTTTAGGAAC CCAGCAGCCC CAGCCCCAGC CCCAGCAGCA GCCCAGTAAC
    AGCCTGGCAT CCAGCTCTTT CTATGGCAAC AACTCTCTGA GCAGCAATTC ACAGAGCAGC
    TCCCTCTTCT CCCAGGGCTC TGCCCAGCCT GCCAACACAT CCTTGGGATT TGGAAGTAGC
    AGTTCTCTCG GCGCCACCCT GGGATCAGCC CTTGGAGGGT TTGGAACAGC AGTTGCAAAC
    TCCAACACTG GCAGTGGCTC CCGCCGTGAC TCCCTGACTG GCAGCAGTGA CCTTTATAAG
    AGGACATCGA GCAGCTTGAC CCCCATTGGA CACAGTTTTT ATAACGGCCT TAGCTTTTCC
    TCCTCTCCTG GACCCGTGGG CATGCCTCTC CCTAGTCAGG GACCAGGACA TTCACAGACA
    CCACCTCCTT CCCTCTCTTC ACATGGATCC TCTTCAAGCT TAAACCTGGG AGGCCTCACC
    AATGGCAGTG GGAGATACAT CTCTGCCGCG CCAGGCGCTG AAGCCAAGTA CCGCAGCGCG
    AGCAGTGCCT CCAGCCTCTT CAGCCCCAGC AGCACCCTGT TTTCTTCTTC TCGGTTGCGA
    TATGGAATGT CTGATGTCAT GCCCTCTGGC AGGAGCAGGC TTCTGGAAGA TTTCCGAAAT
    AACCGGTACC CCAATTTACA ACTGCGGGAG ATTGCTGGGC ATATAATGGA GTTTTCCCAA
    GACCAGCATG GGTCCAGATT CATTCAGCTG AAACTAGAAC GAGCCACACC AGCTGAACGC
    CAGCTTGTCT TCAATGAAAT CCTCCAGGCC GCTTACCAGC TAATGGTGGA TGTATTTGGA
    AATTACGTCA TTCAAAAGTT CTTTGAATTT GGCAGCCTAG AACAGAAGCT GGCTTTGGCA
    GAACGGATTC GAGGCCACGT CCTATCATTG GCACTGCAGA TGTATGGCTG CCGTGTTATC
    CAGAAAGCTC TTGAATTTAT TCCCTCAGAC CAGCAGGTAA TTAATGAGAT GGTTCGGGAG
    CTAGACGGCC ACGTCCTGAA GTGTGTGAAA GACCAGAATG GTAATCACGT GGTTCAGAAG
    TGCATCGAAT GTGTGCAGCC CCAGTCTTTG CAGTTTATTA TTGATGCGTT TAAGGGGCAG
    GTATTTGCTT TGTCCACGCA TCCTTATGGC TGCCGAGTGA TTCAGAGGAT CCTGGAGCAC
    TGTCTCCCTG ACCAGACACT CCCTATTTTA GAGGAGCTTC ACCAGCACAC AGAGCAACTT
    GTACAGGATC AGTATGGAAA TTATGTGATT CAGCACGTAT TGGAGCATGG CCGTCCTGAG
    GACAAAAGTA AAATTGTAGC AGAAATCCGA GGCAATGTGC TTGTATTGAG TCAGCACAAA
    TTTGCAAGCA ACGTTGTGGA GAAGTGTGTC ACTCATGCGT CCCGCACAGA GCGTGCTGTG
    CTTATCGACG AGGTATGCAC CATGAATGAC GGTCCCCACA GTGCCTTATA CACCATGATG
    AAGGACCAGT ATGCCAACTA CGTGGTCCAG AAGATGATCG ATGTGGCAGA GCCAGCCCAG
    CGCAAGATCG TCATGCATAA GATCCGGCCC CACATTGCGA CTCTCCGCAA ATACACCTAT
    GGCAAGCACA TTTTGGCCAA GCTGGAGAAA TACTACATGA AGAATGGCGT TGACTTAGGG
    CCCATCTGTG GCCCCCCTAA TGGTATTATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268095.1
    CDS78..3650
    Translation

    Target ORF information:

    RefSeq Version XM_022412387.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GCCCGGCTGT GGTCCCTCAC
    CAGTATTATG GAGTCACTCC CTGGGGAGTC TACCCAGCCA GTCTCTTCCA GCAGCAAGCA
    GCTGCTGCTG CGGCAGCAAC TAATTCTGCA AATCAGCAGA CTACCCCACA GGCTCAGCAA
    GGGCAGCAGC AGGTTCTCCG TGGAGGAGCC AGCCAGCGTC CTTTGACCCC AAATCAGAAC
    CAGCAGGGAC AGCAAACAGA TCCTCTGGTG GCAGCTGCAG CAGTGAATTC TGCCCTTGCT
    TTTGGACAAG GACTGGCTGC AGGCATGCCA GGTTACCCGG TCTTGGCTCC TGCTGCTTAC
    TATGACCAAA CCGGCGCCCT TGTGGTGAAT GCAGGAGCGA GAAATGGTCT TGGCGCTCCT
    GTTCGGCTGG TAGCTCCTGC CCCAGTCATT ATCAGTTCCT CGGCTGCACA AGCAGCTGTT
    GCGGCGGCCG CAGCTTCAGC AAATGGAGCA GCCGGTGGTC TTGCTGGAAC AACAAATGGA
    CCATTTCGCC CTTTAGGAAC CCAGCAGCCC CAGCCCCAGC CCCAGCAGCA GCCCAGTAAC
    AGCCTGGCAT CCAGCTCTTT CTATGGCAAC AACTCTCTGA GCAGCAATTC ACAGAGCAGC
    TCCCTCTTCT CCCAGGGCTC TGCCCAGCCT GCCAACACAT CCTTGGGATT TGGAAGTAGC
    AGTTCTCTCG GCGCCACCCT GGGATCAGCC CTTGGAGGGT TTGGAACAGC AGTTGCAAAC
    TCCAACACTG GCAGTGGCTC CCGCCGTGAC TCCCTGACTG GCAGCAGTGA CCTTTATAAG
    AGGACATCGA GCAGCTTGAC CCCCATTGGA CACAGTTTTT ATAACGGCCT TAGCTTTTCC
    TCCTCTCCTG GACCCGTGGG CATGCCTCTC CCTAGTCAGG GACCAGGACA TTCACAGACA
    CCACCTCCTT CCCTCTCTTC ACATGGATCC TCTTCAAGCT TAAACCTGGG AGGCCTCACC
    AATGGCAGTG GGAGATACAT CTCTGCCGCG CCAGGCGCTG AAGCCAAGTA CCGCAGCGCG
    AGCAGTGCCT CCAGCCTCTT CAGCCCCAGC AGCACCCTGT TTTCTTCTTC TCGGTTGCGA
    TATGGAATGT CTGATGTCAT GCCCTCTGGC AGGAGCAGGC TTCTGGAAGA TTTCCGAAAT
    AACCGGTACC CCAATTTACA ACTGCGGGAG ATTGCTGGGC ATATAATGGA GTTTTCCCAA
    GACCAGCATG GGTCCAGATT CATTCAGCTG AAACTAGAAC GAGCCACACC AGCTGAACGC
    CAGCTTGTCT TCAATGAAAT CCTCCAGGCC GCTTACCAGC TAATGGTGGA TGTATTTGGA
    AATTACGTCA TTCAAAAGTT CTTTGAATTT GGCAGCCTAG AACAGAAGCT GGCTTTGGCA
    GAACGGATTC GAGGCCACGT CCTATCATTG GCACTGCAGA TGTATGGCTG CCGTGTTATC
    CAGAAAGCTC TTGAATTTAT TCCCTCAGAC CAGCAGGTAA TTAATGAGAT GGTTCGGGAG
    CTAGACGGCC ACGTCCTGAA GTGTGTGAAA GACCAGAATG GTAATCACGT GGTTCAGAAG
    TGCATCGAAT GTGTGCAGCC CCAGTCTTTG CAGTTTATTA TTGATGCGTT TAAGGGGCAG
    GTATTTGCTT TGTCCACGCA TCCTTATGGC TGCCGAGTGA TTCAGAGGAT CCTGGAGCAC
    TGTCTCCCTG ACCAGACACT CCCTATTTTA GAGGAGCTTC ACCAGCACAC AGAGCAACTT
    GTACAGGATC AGTATGGAAA TTATGTGATT CAGCACGTAT TGGAGCATGG CCGTCCTGAG
    GACAAAAGTA AAATTGTAGC AGAAATCCGA GGCAATGTGC TTGTATTGAG TCAGCACAAA
    TTTGCAAGCA ACGTTGTGGA GAAGTGTGTC ACTCATGCGT CCCGCACAGA GCGTGCTGTG
    CTTATCGACG AGGTATGCAC CATGAATGAC GGTCCCCACA GTGCCTTATA CACCATGATG
    AAGGACCAGT ATGCCAACTA CGTGGTCCAG AAGATGATCG ATGTGGCAGA GCCAGCCCAG
    CGCAAGATCG TCATGCATAA GATCCGGCCC CACATTGCGA CTCTCCGCAA ATACACCTAT
    GGCAAGCACA TTTTGGCCAA GCTGGAGAAA TACTACATGA AGAATGGCGT TGACTTAGGG
    CCCATCTGTG GCCCCCCTAA TGGTATTATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286885
    Clone ID Related Accession (Same CDS sequence) XM_022412389.1
    Accession Version XM_022412389.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTGTGTTTTC AGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGCTGTTGCG
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACCCA GCAGCCCCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAGC
    CTGGCATCCA GCTCTTTCTA TGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCTGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGG AGGCCTCACC
    AATGGCAGTG GGAGATACAT CTCTGCCGCG CCAGGCGCTG AAGCCAAGTA CCGCAGCGCG
    AGCAGTGCCT CCAGCCTCTT CAGCCCCAGC AGCACCCTGT TTTCTTCTTC TCGGTTGCGA
    TATGGAATGT CTGATGTCAT GCCCTCTGGC AGGAGCAGGC TTCTGGAAGA TTTCCGAAAT
    AACCGGTACC CCAATTTACA ACTGCGGGAG ATTGCTGGGC ATATAATGGA GTTTTCCCAA
    GACCAGCATG GGTCCAGATT CATTCAGCTG AAACTAGAAC GAGCCACACC AGCTGAACGC
    CAGCTTGTCT TCAATGAAAT CCTCCAGGCC GCTTACCAGC TAATGGTGGA TGTATTTGGA
    AATTACGTCA TTCAAAAGTT CTTTGAATTT GGCAGCCTAG AACAGAAGCT GGCTTTGGCA
    GAACGGATTC GAGGCCACGT CCTATCATTG GCACTGCAGA TGTATGGCTG CCGTGTTATC
    CAGAAAGCTC TTGAATTTAT TCCCTCAGAC CAGCAGGTAA TTAATGAGAT GGTTCGGGAG
    CTAGACGGCC ACGTCCTGAA GTGTGTGAAA GACCAGAATG GTAATCACGT GGTTCAGAAG
    TGCATCGAAT GTGTGCAGCC CCAGTCTTTG CAGTTTATTA TTGATGCGTT TAAGGGGCAG
    GTATTTGCTT TGTCCACGCA TCCTTATGGC TGCCGAGTGA TTCAGAGGAT CCTGGAGCAC
    TGTCTCCCTG ACCAGACACT CCCTATTTTA GAGGAGCTTC ACCAGCACAC AGAGCAACTT
    GTACAGGATC AGTATGGAAA TTATGTGATT CAGCACGTAT TGGAGCATGG CCGTCCTGAG
    GACAAAAGTA AAATTGTAGC AGAAATCCGA GGCAATGTGC TTGTATTGAG TCAGCACAAA
    TTTGCAAGCA ACGTTGTGGA GAAGTGTGTC ACTCATGCGT CCCGCACAGA GCGTGCTGTG
    CTTATCGACG AGGTATGCAC CATGAATGAC GGTCCCCACA GTGCCTTATA CACCATGATG
    AAGGACCAGT ATGCCAACTA CGTGGTCCAG AAGATGATCG ATGTGGCAGA GCCAGCCCAG
    CGCAAGATCG TCATGCATAA GATCCGGCCC CACATTGCGA CTCTCCGCAA ATACACCTAT
    GGCAAGCACA TTTTGGCCAA GCTGGAGAAA TACTACATGA AGAATGGCGT TGACTTAGGG
    CCCATCTGTG GCCCCCCTAA TGGTATTATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268097.1
    CDS80..3532
    Translation

    Target ORF information:

    RefSeq Version XM_022412389.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTGTGTTTTC AGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGCTGTTGCG
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACCCA GCAGCCCCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAGC
    CTGGCATCCA GCTCTTTCTA TGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCTGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGG AGGCCTCACC
    AATGGCAGTG GGAGATACAT CTCTGCCGCG CCAGGCGCTG AAGCCAAGTA CCGCAGCGCG
    AGCAGTGCCT CCAGCCTCTT CAGCCCCAGC AGCACCCTGT TTTCTTCTTC TCGGTTGCGA
    TATGGAATGT CTGATGTCAT GCCCTCTGGC AGGAGCAGGC TTCTGGAAGA TTTCCGAAAT
    AACCGGTACC CCAATTTACA ACTGCGGGAG ATTGCTGGGC ATATAATGGA GTTTTCCCAA
    GACCAGCATG GGTCCAGATT CATTCAGCTG AAACTAGAAC GAGCCACACC AGCTGAACGC
    CAGCTTGTCT TCAATGAAAT CCTCCAGGCC GCTTACCAGC TAATGGTGGA TGTATTTGGA
    AATTACGTCA TTCAAAAGTT CTTTGAATTT GGCAGCCTAG AACAGAAGCT GGCTTTGGCA
    GAACGGATTC GAGGCCACGT CCTATCATTG GCACTGCAGA TGTATGGCTG CCGTGTTATC
    CAGAAAGCTC TTGAATTTAT TCCCTCAGAC CAGCAGGTAA TTAATGAGAT GGTTCGGGAG
    CTAGACGGCC ACGTCCTGAA GTGTGTGAAA GACCAGAATG GTAATCACGT GGTTCAGAAG
    TGCATCGAAT GTGTGCAGCC CCAGTCTTTG CAGTTTATTA TTGATGCGTT TAAGGGGCAG
    GTATTTGCTT TGTCCACGCA TCCTTATGGC TGCCGAGTGA TTCAGAGGAT CCTGGAGCAC
    TGTCTCCCTG ACCAGACACT CCCTATTTTA GAGGAGCTTC ACCAGCACAC AGAGCAACTT
    GTACAGGATC AGTATGGAAA TTATGTGATT CAGCACGTAT TGGAGCATGG CCGTCCTGAG
    GACAAAAGTA AAATTGTAGC AGAAATCCGA GGCAATGTGC TTGTATTGAG TCAGCACAAA
    TTTGCAAGCA ACGTTGTGGA GAAGTGTGTC ACTCATGCGT CCCGCACAGA GCGTGCTGTG
    CTTATCGACG AGGTATGCAC CATGAATGAC GGTCCCCACA GTGCCTTATA CACCATGATG
    AAGGACCAGT ATGCCAACTA CGTGGTCCAG AAGATGATCG ATGTGGCAGA GCCAGCCCAG
    CGCAAGATCG TCATGCATAA GATCCGGCCC CACATTGCGA CTCTCCGCAA ATACACCTAT
    GGCAAGCACA TTTTGGCCAA GCTGGAGAAA TACTACATGA AGAATGGCGT TGACTTAGGG
    CCCATCTGTG GCCCCCCTAA TGGTATTATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286886
    Clone ID Related Accession (Same CDS sequence) XM_022412392.1
    Accession Version XM_022412392.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTGTGTTTTC AGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGCTGTTGCG
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACCCA GCAGCCCCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAGC
    CTGGCATCCA GCTCTTTCTA TGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCTGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGG AGGCCTCACC
    AATGGCAGTG GGAGATACAT CTCTGCCGCG CCAGGCGCTG AAGCCAAGTA CCGCAGCGCG
    AGCAGTGCCT CCAGCCTCTT CAGCCCCAGC AGCACCCTGT TTTCTTCTTC TCGGTTGCGA
    TATGGAATGT CTGATGTCAT GCCCTCTGGC AGGAGCAGGC TTCTGGAAGA TTTCCGAAAT
    AACCGGTACC CCAATTTACA ACTGCGGGAG ATTGCTGGGC ATATAATGGA GTTTTCCCAA
    GACCAGCATG GGTCCAGATT CATTCAGCTG AAACTAGAAC GAGCCACACC AGCTGAACGC
    CAGCTTGTCT TCAATGAAAT CCTCCAGGCC GCTTACCAGC TAATGGTGGA TGTATTTGGA
    AATTACGTCA TTCAAAAGTT CTTTGAATTT GGCAGCCTAG AACAGAAGCT GGCTTTGGCA
    GAACGGATTC GAGGCCACGT CCTATCATTG GCACTGCAGA TGTATGGCTG CCGTGTTATC
    CAGAAAGCTC TTGAATTTAT TCCCTCAGAC CAGCAGAATG AGATGGTTCG GGAGCTAGAC
    GGCCACGTCC TGAAGTGTGT GAAAGACCAG AATGGTAATC ACGTGGTTCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TTTGCAGTTT ATTATTGATG CGTTTAAGGG GCAGGTATTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATCCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTATGT GATTCAGCAC GTATTGGAGC ATGGCCGTCC TGAGGACAAA
    AGTAAAATTG TAGCAGAAAT CCGAGGCAAT GTGCTTGTAT TGAGTCAGCA CAAATTTGCA
    AGCAACGTTG TGGAGAAGTG TGTCACTCAT GCGTCCCGCA CAGAGCGTGC TGTGCTTATC
    GACGAGGTAT GCACCATGAA TGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGCGCAAG
    ATCGTCATGC ATAAGATCCG GCCCCACATT GCGACTCTCC GCAAATACAC CTATGGCAAG
    CACATTTTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGTGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268100.1
    CDS80..3526
    Translation

    Target ORF information:

    RefSeq Version XM_022412392.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412392.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT CCAGCAAAGA TGGACCAAAA
    GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG GAACATCAGA TCATTCAGTT
    TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT TCCATGTGAA CAGTGAGGTC
    AATTCTGTAC TGTCCCCACG ATCGGAGAGT GGGGGACTAG GCGTTAGCAT GGTGGAGTAT
    GTGTTGAGCT CATCCCCGGG CGATTCCTGT CTAAGAAAAG GAGGATTTGG CCCAAGGGAT
    GCAGACAGTG ATGAAAACGA CAAAGGTGAC AAGAAGAACA AGGGTGCGTT TGATGGAGAT
    AAGCTAGGAG ATTTGAAGGA GGAGGGTGAT GTGATGGACA AGACGAATGG TTTGCCAGTG
    CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA CCCCTGGTAA TTGCCAGAAC
    TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG GTTCTGAGGG CTTAGCCCAG
    CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT CCAACATGGA GTCCCAGAGT
    GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC TTCAGTTTGA TTATTCAGGC
    ACGCAGGTAC CTGTGGACTC AGCAGCAGCA ACTGTGGGAC TTTTTGACTA CAATTCTCAA
    CAACAGCTGT TCCAAAGACC TAATGCACTT GCTGTCCAGC AGTTGACAGC TGCCCAGCAG
    CAGCAGTATG CACTGGCAGC GGCTCATCAA CCTCACATCG GTGTGTTTTC AGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGCTGTTGCG
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACCCA GCAGCCCCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAGC
    CTGGCATCCA GCTCTTTCTA TGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCTGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGG AGGCCTCACC
    AATGGCAGTG GGAGATACAT CTCTGCCGCG CCAGGCGCTG AAGCCAAGTA CCGCAGCGCG
    AGCAGTGCCT CCAGCCTCTT CAGCCCCAGC AGCACCCTGT TTTCTTCTTC TCGGTTGCGA
    TATGGAATGT CTGATGTCAT GCCCTCTGGC AGGAGCAGGC TTCTGGAAGA TTTCCGAAAT
    AACCGGTACC CCAATTTACA ACTGCGGGAG ATTGCTGGGC ATATAATGGA GTTTTCCCAA
    GACCAGCATG GGTCCAGATT CATTCAGCTG AAACTAGAAC GAGCCACACC AGCTGAACGC
    CAGCTTGTCT TCAATGAAAT CCTCCAGGCC GCTTACCAGC TAATGGTGGA TGTATTTGGA
    AATTACGTCA TTCAAAAGTT CTTTGAATTT GGCAGCCTAG AACAGAAGCT GGCTTTGGCA
    GAACGGATTC GAGGCCACGT CCTATCATTG GCACTGCAGA TGTATGGCTG CCGTGTTATC
    CAGAAAGCTC TTGAATTTAT TCCCTCAGAC CAGCAGAATG AGATGGTTCG GGAGCTAGAC
    GGCCACGTCC TGAAGTGTGT GAAAGACCAG AATGGTAATC ACGTGGTTCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TTTGCAGTTT ATTATTGATG CGTTTAAGGG GCAGGTATTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATCCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTATGT GATTCAGCAC GTATTGGAGC ATGGCCGTCC TGAGGACAAA
    AGTAAAATTG TAGCAGAAAT CCGAGGCAAT GTGCTTGTAT TGAGTCAGCA CAAATTTGCA
    AGCAACGTTG TGGAGAAGTG TGTCACTCAT GCGTCCCGCA CAGAGCGTGC TGTGCTTATC
    GACGAGGTAT GCACCATGAA TGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGCGCAAG
    ATCGTCATGC ATAAGATCCG GCCCCACATT GCGACTCTCC GCAAATACAC CTATGGCAAG
    CACATTTTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGTGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286887
    Clone ID Related Accession (Same CDS sequence) XM_022412393.1
    Accession Version XM_022412393.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGCCTCACCA ATGGCAGTGG GAGATACATC TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTGTT TTCTTCTTCT
    CGGTTGCGAT ATGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT
    TTCCGAAATA ACCGGTACCC CAATTTACAA CTGCGGGAGA TTGCTGGGCA TATAATGGAG
    TTTTCCCAAG ACCAGCATGG GTCCAGATTC ATTCAGCTGA AACTAGAACG AGCCACACCA
    GCTGAACGCC AGCTTGTCTT CAATGAAATC CTCCAGGCCG CTTACCAGCT AATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAAAAGTTC TTTGAATTTG GCAGCCTAGA ACAGAAGCTG
    GCTTTGGCAG AACGGATTCG AGGCCACGTC CTATCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAATTTATT CCCTCAGACC AGCAGGTAAT TAATGAGATG
    GTTCGGGAGC TAGACGGCCA CGTCCTGAAG TGTGTGAAAG ACCAGAATGG TAATCACGTG
    GTTCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTTTGC AGTTTATTAT TGATGCGTTT
    AAGGGGCAGG TATTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATC
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TATGTGATTC AGCACGTATT GGAGCATGGC
    CGTCCTGAGG ACAAAAGTAA AATTGTAGCA GAAATCCGAG GCAATGTGCT TGTATTGAGT
    CAGCACAAAT TTGCAAGCAA CGTTGTGGAG AAGTGTGTCA CTCATGCGTC CCGCACAGAG
    CGTGCTGTGC TTATCGACGA GGTATGCACC ATGAATGACG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGC GCAAGATCGT CATGCATAAG ATCCGGCCCC ACATTGCGAC TCTCCGCAAA
    TACACCTATG GCAAGCACAT TTTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGTGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268101.1
    CDS81..3482
    Translation

    Target ORF information:

    RefSeq Version XM_022412393.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412393.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGCCTCACCA ATGGCAGTGG GAGATACATC TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTGTT TTCTTCTTCT
    CGGTTGCGAT ATGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT
    TTCCGAAATA ACCGGTACCC CAATTTACAA CTGCGGGAGA TTGCTGGGCA TATAATGGAG
    TTTTCCCAAG ACCAGCATGG GTCCAGATTC ATTCAGCTGA AACTAGAACG AGCCACACCA
    GCTGAACGCC AGCTTGTCTT CAATGAAATC CTCCAGGCCG CTTACCAGCT AATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAAAAGTTC TTTGAATTTG GCAGCCTAGA ACAGAAGCTG
    GCTTTGGCAG AACGGATTCG AGGCCACGTC CTATCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAATTTATT CCCTCAGACC AGCAGGTAAT TAATGAGATG
    GTTCGGGAGC TAGACGGCCA CGTCCTGAAG TGTGTGAAAG ACCAGAATGG TAATCACGTG
    GTTCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTTTGC AGTTTATTAT TGATGCGTTT
    AAGGGGCAGG TATTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATC
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TATGTGATTC AGCACGTATT GGAGCATGGC
    CGTCCTGAGG ACAAAAGTAA AATTGTAGCA GAAATCCGAG GCAATGTGCT TGTATTGAGT
    CAGCACAAAT TTGCAAGCAA CGTTGTGGAG AAGTGTGTCA CTCATGCGTC CCGCACAGAG
    CGTGCTGTGC TTATCGACGA GGTATGCACC ATGAATGACG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGC GCAAGATCGT CATGCATAAG ATCCGGCCCC ACATTGCGAC TCTCCGCAAA
    TACACCTATG GCAAGCACAT TTTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGTGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286888
    Clone ID Related Accession (Same CDS sequence) XM_022412399.1
    Accession Version XM_022412399.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTTTAGCG
    CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGTGCTG CTCCCCCGGG GACGGACCCC
    TACACAGCTG GACTGGCTGC AGCGGCGACA CTAGGCCCGG CTGTGGTCCC TCACCAGTAT
    TATGGAGTCA CTCCCTGGGG AGTCTACCCA GCCAGTCTCT TCCAGCAGCA AGCAGCTGCT
    GCTGCGGCAG CAACTAATTC TGCAAATCAG CAGACTACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CCCCAAATCA GAACCAGCAG
    GGACAGCAAA CAGATCCTCT GGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCTTTTGGA
    CAAGGACTGG CTGCAGGCAT GCCAGGTTAC CCGGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGCG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGCGC TCCTGTTCGG
    CTGGTAGCTC CTGCCCCAGT CATTATCAGT TCCTCGGCTG CACAAGCAGC TGTTGCGGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACCCAGCA GCCCCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAGCCTG
    GCATCCAGCT CTTTCTATGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGCCT CACCAATGGC
    AGTGGGAGAT ACATCTCTGC CGCGCCAGGC GCTGAAGCCA AGTACCGCAG CGCGAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTGTTTTCTT CTTCTCGGTT GCGATATGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTCTGG AAGATTTCCG AAATAACCGG
    TACCCCAATT TACAACTGCG GGAGATTGCT GGGCATATAA TGGAGTTTTC CCAAGACCAG
    CATGGGTCCA GATTCATTCA GCTGAAACTA GAACGAGCCA CACCAGCTGA ACGCCAGCTT
    GTCTTCAATG AAATCCTCCA GGCCGCTTAC CAGCTAATGG TGGATGTATT TGGAAATTAC
    GTCATTCAAA AGTTCTTTGA ATTTGGCAGC CTAGAACAGA AGCTGGCTTT GGCAGAACGG
    ATTCGAGGCC ACGTCCTATC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAAT TTATTCCCTC AGACCAGCAG GTAATTAATG AGATGGTTCG GGAGCTAGAC
    GGCCACGTCC TGAAGTGTGT GAAAGACCAG AATGGTAATC ACGTGGTTCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TTTGCAGTTT ATTATTGATG CGTTTAAGGG GCAGGTATTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATCCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTATGT GATTCAGCAC GTATTGGAGC ATGGCCGTCC TGAGGACAAA
    AGTAAAATTG TAGCAGAAAT CCGAGGCAAT GTGCTTGTAT TGAGTCAGCA CAAATTTGCA
    AGCAACGTTG TGGAGAAGTG TGTCACTCAT GCGTCCCGCA CAGAGCGTGC TGTGCTTATC
    GACGAGGTAT GCACCATGAA TGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGCGCAAG
    ATCGTCATGC ATAAGATCCG GCCCCACATT GCGACTCTCC GCAAATACAC CTATGGCAAG
    CACATTTTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGTGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268107.1
    CDS80..3466
    Translation

    Target ORF information:

    RefSeq Version XM_022412399.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412399.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCGCTGC CACCGCCGGA GCCGGGGCCA GCGCCTATCC CAGGATGCAC CGCGCCAGCC 
    CCGTCCCCAG TGGGCCGCCA TGTTGTCGGA GTGAAAGGTG TTGGTGGAAT GAGCGTTGCA
    TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT TCAGCCCCCA CCTGAAACAT
    CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT TGACATCTGG AACAGGGTCG
    CAAGCGCAGC CACAACCAGC TGCAAATCAG GCTCTTGCAG CTGGGACACA CTCCAGCCCT
    GTCCCAGGAT CCATAGGAGT TGCAGGACGA TCCCAAGACG ACGCTATGGT GGACTACTTC
    TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGCA GTGGTGGAGG CGGCTATAAT
    AATAGCAAAC ATCGATGGCC TACTGGGGAT AACATTCATG CAGAACATCA GGTACGTTCT
    ATGGATGAGC TGAATCATGA TTTTCAAGCA CTTGCTCTGG AGGGAAGAGC GATGGGAGAG
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTTTAGCG
    CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGTGCTG CTCCCCCGGG GACGGACCCC
    TACACAGCTG GACTGGCTGC AGCGGCGACA CTAGGCCCGG CTGTGGTCCC TCACCAGTAT
    TATGGAGTCA CTCCCTGGGG AGTCTACCCA GCCAGTCTCT TCCAGCAGCA AGCAGCTGCT
    GCTGCGGCAG CAACTAATTC TGCAAATCAG CAGACTACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CCCCAAATCA GAACCAGCAG
    GGACAGCAAA CAGATCCTCT GGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCTTTTGGA
    CAAGGACTGG CTGCAGGCAT GCCAGGTTAC CCGGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGCG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGCGC TCCTGTTCGG
    CTGGTAGCTC CTGCCCCAGT CATTATCAGT TCCTCGGCTG CACAAGCAGC TGTTGCGGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACCCAGCA GCCCCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAGCCTG
    GCATCCAGCT CTTTCTATGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGCCT CACCAATGGC
    AGTGGGAGAT ACATCTCTGC CGCGCCAGGC GCTGAAGCCA AGTACCGCAG CGCGAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTGTTTTCTT CTTCTCGGTT GCGATATGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTCTGG AAGATTTCCG AAATAACCGG
    TACCCCAATT TACAACTGCG GGAGATTGCT GGGCATATAA TGGAGTTTTC CCAAGACCAG
    CATGGGTCCA GATTCATTCA GCTGAAACTA GAACGAGCCA CACCAGCTGA ACGCCAGCTT
    GTCTTCAATG AAATCCTCCA GGCCGCTTAC CAGCTAATGG TGGATGTATT TGGAAATTAC
    GTCATTCAAA AGTTCTTTGA ATTTGGCAGC CTAGAACAGA AGCTGGCTTT GGCAGAACGG
    ATTCGAGGCC ACGTCCTATC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAAT TTATTCCCTC AGACCAGCAG GTAATTAATG AGATGGTTCG GGAGCTAGAC
    GGCCACGTCC TGAAGTGTGT GAAAGACCAG AATGGTAATC ACGTGGTTCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TTTGCAGTTT ATTATTGATG CGTTTAAGGG GCAGGTATTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATCCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTATGT GATTCAGCAC GTATTGGAGC ATGGCCGTCC TGAGGACAAA
    AGTAAAATTG TAGCAGAAAT CCGAGGCAAT GTGCTTGTAT TGAGTCAGCA CAAATTTGCA
    AGCAACGTTG TGGAGAAGTG TGTCACTCAT GCGTCCCGCA CAGAGCGTGC TGTGCTTATC
    GACGAGGTAT GCACCATGAA TGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGCGCAAG
    ATCGTCATGC ATAAGATCCG GCCCCACATT GCGACTCTCC GCAAATACAC CTATGGCAAG
    CACATTTTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGTGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35441
    Clone ID Related Accession (Same CDS sequence) XM_014109998.1
    Accession Version XM_014109998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGCCTCACCA ATGGCAGTGG GAGATACATC TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTGTT TTCTTCTTCT
    CGGTTGCGAT ATGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT
    TTCCGAAATA ACCGGTACCC CAATTTACAA CTGCGGGAGA TTGCTGGGCA TATAATGGAG
    TTTTCCCAAG ACCAGCATGG GTCCAGATTC ATTCAGCTGA AACTAGAACG AGCCACACCA
    GCTGAACGCC AGCTTGTCTT CAATGAAATC CTCCAGGCCG CTTACCAGCT AATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAAAAGTTC TTTGAATTTG GCAGCCTAGA ACAGAAGCTG
    GCTTTGGCAG AACGGATTCG AGGCCACGTC CTATCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAATTTATT CCCTCAGACC AGCAGAATGA GATGGTTCGG
    GAGCTAGACG GCCACGTCCT GAAGTGTGTG AAAGACCAGA ATGGTAATCA CGTGGTTCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT TTGCAGTTTA TTATTGATGC GTTTAAGGGG
    CAGGTATTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATCCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTATGTG ATTCAGCACG TATTGGAGCA TGGCCGTCCT
    GAGGACAAAA GTAAAATTGT AGCAGAAATC CGAGGCAATG TGCTTGTATT GAGTCAGCAC
    AAATTTGCAA GCAACGTTGT GGAGAAGTGT GTCACTCATG CGTCCCGCAC AGAGCGTGCT
    GTGCTTATCG ACGAGGTATG CACCATGAAT GACGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGCGCAAGA TCGTCATGCA TAAGATCCGG CCCCACATTG CGACTCTCCG CAAATACACC
    TATGGCAAGC ACATTTTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GTGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965473.1
    CDS287..3862
    Translation

    Target ORF information:

    RefSeq Version XM_014109998.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGCCTCACCA ATGGCAGTGG GAGATACATC TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTGTT TTCTTCTTCT
    CGGTTGCGAT ATGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT
    TTCCGAAATA ACCGGTACCC CAATTTACAA CTGCGGGAGA TTGCTGGGCA TATAATGGAG
    TTTTCCCAAG ACCAGCATGG GTCCAGATTC ATTCAGCTGA AACTAGAACG AGCCACACCA
    GCTGAACGCC AGCTTGTCTT CAATGAAATC CTCCAGGCCG CTTACCAGCT AATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAAAAGTTC TTTGAATTTG GCAGCCTAGA ACAGAAGCTG
    GCTTTGGCAG AACGGATTCG AGGCCACGTC CTATCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAATTTATT CCCTCAGACC AGCAGAATGA GATGGTTCGG
    GAGCTAGACG GCCACGTCCT GAAGTGTGTG AAAGACCAGA ATGGTAATCA CGTGGTTCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT TTGCAGTTTA TTATTGATGC GTTTAAGGGG
    CAGGTATTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATCCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTATGTG ATTCAGCACG TATTGGAGCA TGGCCGTCCT
    GAGGACAAAA GTAAAATTGT AGCAGAAATC CGAGGCAATG TGCTTGTATT GAGTCAGCAC
    AAATTTGCAA GCAACGTTGT GGAGAAGTGT GTCACTCATG CGTCCCGCAC AGAGCGTGCT
    GTGCTTATCG ACGAGGTATG CACCATGAAT GACGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGCGCAAGA TCGTCATGCA TAAGATCCGG CCCCACATTG CGACTCTCCG CAAATACACC
    TATGGCAAGC ACATTTTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GTGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35442
    Clone ID Related Accession (Same CDS sequence) XM_014110005.1
    Accession Version XM_014110005.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGCCTCA CCAATGGCAG TGGGAGATAC ATCTCTGCCG CGCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CGAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT GTTTTCTTCT
    TCTCGGTTGC GATATGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTCTGGAA
    GATTTCCGAA ATAACCGGTA CCCCAATTTA CAACTGCGGG AGATTGCTGG GCATATAATG
    GAGTTTTCCC AAGACCAGCA TGGGTCCAGA TTCATTCAGC TGAAACTAGA ACGAGCCACA
    CCAGCTGAAC GCCAGCTTGT CTTCAATGAA ATCCTCCAGG CCGCTTACCA GCTAATGGTG
    GATGTATTTG GAAATTACGT CATTCAAAAG TTCTTTGAAT TTGGCAGCCT AGAACAGAAG
    CTGGCTTTGG CAGAACGGAT TCGAGGCCAC GTCCTATCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAATTT ATTCCCTCAG ACCAGCAGGT AATTAATGAG
    ATGGTTCGGG AGCTAGACGG CCACGTCCTG AAGTGTGTGA AAGACCAGAA TGGTAATCAC
    GTGGTTCAGA AGTGCATCGA ATGTGTGCAG CCCCAGTCTT TGCAGTTTAT TATTGATGCG
    TTTAAGGGGC AGGTATTTGC TTTGTCCACG CATCCTTATG GCTGCCGAGT GATTCAGAGG
    ATCCTGGAGC ACTGTCTCCC TGACCAGACA CTCCCTATTT TAGAGGAGCT TCACCAGCAC
    ACAGAGCAAC TTGTACAGGA TCAGTATGGA AATTATGTGA TTCAGCACGT ATTGGAGCAT
    GGCCGTCCTG AGGACAAAAG TAAAATTGTA GCAGAAATCC GAGGCAATGT GCTTGTATTG
    AGTCAGCACA AATTTGCAAG CAACGTTGTG GAGAAGTGTG TCACTCATGC GTCCCGCACA
    GAGCGTGCTG TGCTTATCGA CGAGGTATGC ACCATGAATG ACGGTCCCCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAC TACGTGGTCC AGAAGATGAT CGATGTGGCA
    GAGCCAGCCC AGCGCAAGAT CGTCATGCAT AAGATCCGGC CCCACATTGC GACTCTCCGC
    AAATACACCT ATGGCAAGCA CATTTTGGCC AAGCTGGAGA AATACTACAT GAAGAATGGC
    GTTGACTTAG GGCCCATCTG TGGCCCCCCT AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965480.1
    CDS287..3631
    Translation

    Target ORF information:

    RefSeq Version XM_014110005.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110005.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGCCTCA CCAATGGCAG TGGGAGATAC ATCTCTGCCG CGCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CGAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT GTTTTCTTCT
    TCTCGGTTGC GATATGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTCTGGAA
    GATTTCCGAA ATAACCGGTA CCCCAATTTA CAACTGCGGG AGATTGCTGG GCATATAATG
    GAGTTTTCCC AAGACCAGCA TGGGTCCAGA TTCATTCAGC TGAAACTAGA ACGAGCCACA
    CCAGCTGAAC GCCAGCTTGT CTTCAATGAA ATCCTCCAGG CCGCTTACCA GCTAATGGTG
    GATGTATTTG GAAATTACGT CATTCAAAAG TTCTTTGAAT TTGGCAGCCT AGAACAGAAG
    CTGGCTTTGG CAGAACGGAT TCGAGGCCAC GTCCTATCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAATTT ATTCCCTCAG ACCAGCAGGT AATTAATGAG
    ATGGTTCGGG AGCTAGACGG CCACGTCCTG AAGTGTGTGA AAGACCAGAA TGGTAATCAC
    GTGGTTCAGA AGTGCATCGA ATGTGTGCAG CCCCAGTCTT TGCAGTTTAT TATTGATGCG
    TTTAAGGGGC AGGTATTTGC TTTGTCCACG CATCCTTATG GCTGCCGAGT GATTCAGAGG
    ATCCTGGAGC ACTGTCTCCC TGACCAGACA CTCCCTATTT TAGAGGAGCT TCACCAGCAC
    ACAGAGCAAC TTGTACAGGA TCAGTATGGA AATTATGTGA TTCAGCACGT ATTGGAGCAT
    GGCCGTCCTG AGGACAAAAG TAAAATTGTA GCAGAAATCC GAGGCAATGT GCTTGTATTG
    AGTCAGCACA AATTTGCAAG CAACGTTGTG GAGAAGTGTG TCACTCATGC GTCCCGCACA
    GAGCGTGCTG TGCTTATCGA CGAGGTATGC ACCATGAATG ACGGTCCCCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAC TACGTGGTCC AGAAGATGAT CGATGTGGCA
    GAGCCAGCCC AGCGCAAGAT CGTCATGCAT AAGATCCGGC CCCACATTGC GACTCTCCGC
    AAATACACCT ATGGCAAGCA CATTTTGGCC AAGCTGGAGA AATACTACAT GAAGAATGGC
    GTTGACTTAG GGCCCATCTG TGGCCCCCCT AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35443
    Clone ID Related Accession (Same CDS sequence) XM_014110008.1
    Accession Version XM_014110008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGCCTCA CCAATGGCAG TGGGAGATAC ATCTCTGCCG CGCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CGAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT GTTTTCTTCT
    TCTCGGTTGC GATATGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTCTGGAA
    GATTTCCGAA ATAACCGGTA CCCCAATTTA CAACTGCGGG AGATTGCTGG GCATATAATG
    GAGTTTTCCC AAGACCAGCA TGGGTCCAGA TTCATTCAGC TGAAACTAGA ACGAGCCACA
    CCAGCTGAAC GCCAGCTTGT CTTCAATGAA ATCCTCCAGG CCGCTTACCA GCTAATGGTG
    GATGTATTTG GAAATTACGT CATTCAAAAG TTCTTTGAAT TTGGCAGCCT AGAACAGAAG
    CTGGCTTTGG CAGAACGGAT TCGAGGCCAC GTCCTATCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAATTT ATTCCCTCAG ACCAGCAGAA TGAGATGGTT
    CGGGAGCTAG ACGGCCACGT CCTGAAGTGT GTGAAAGACC AGAATGGTAA TCACGTGGTT
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTTTGCAGT TTATTATTGA TGCGTTTAAG
    GGGCAGGTAT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATCCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAT GTGATTCAGC ACGTATTGGA GCATGGCCGT
    CCTGAGGACA AAAGTAAAAT TGTAGCAGAA ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG
    CACAAATTTG CAAGCAACGT TGTGGAGAAG TGTGTCACTC ATGCGTCCCG CACAGAGCGT
    GCTGTGCTTA TCGACGAGGT ATGCACCATG AATGACGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGCGCA AGATCGTCAT GCATAAGATC CGGCCCCACA TTGCGACTCT CCGCAAATAC
    ACCTATGGCA AGCACATTTT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGTGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965483.1
    CDS287..3625
    Translation

    Target ORF information:

    RefSeq Version XM_014110008.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGCCTCA CCAATGGCAG TGGGAGATAC ATCTCTGCCG CGCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CGAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT GTTTTCTTCT
    TCTCGGTTGC GATATGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTCTGGAA
    GATTTCCGAA ATAACCGGTA CCCCAATTTA CAACTGCGGG AGATTGCTGG GCATATAATG
    GAGTTTTCCC AAGACCAGCA TGGGTCCAGA TTCATTCAGC TGAAACTAGA ACGAGCCACA
    CCAGCTGAAC GCCAGCTTGT CTTCAATGAA ATCCTCCAGG CCGCTTACCA GCTAATGGTG
    GATGTATTTG GAAATTACGT CATTCAAAAG TTCTTTGAAT TTGGCAGCCT AGAACAGAAG
    CTGGCTTTGG CAGAACGGAT TCGAGGCCAC GTCCTATCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAATTT ATTCCCTCAG ACCAGCAGAA TGAGATGGTT
    CGGGAGCTAG ACGGCCACGT CCTGAAGTGT GTGAAAGACC AGAATGGTAA TCACGTGGTT
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTTTGCAGT TTATTATTGA TGCGTTTAAG
    GGGCAGGTAT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATCCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAT GTGATTCAGC ACGTATTGGA GCATGGCCGT
    CCTGAGGACA AAAGTAAAAT TGTAGCAGAA ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG
    CACAAATTTG CAAGCAACGT TGTGGAGAAG TGTGTCACTC ATGCGTCCCG CACAGAGCGT
    GCTGTGCTTA TCGACGAGGT ATGCACCATG AATGACGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGCGCA AGATCGTCAT GCATAAGATC CGGCCCCACA TTGCGACTCT CCGCAAATAC
    ACCTATGGCA AGCACATTTT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGTGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03438
    Clone ID Related Accession (Same CDS sequence) XM_014109987.1 , XM_005617672.2 , XM_014109987.2
    Accession Version XM_014109987.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGCCTCACCA ATGGCAGTGG GAGATACATC TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTGTT TTCTTCTTCT
    CGGTTGCGAT ATGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT
    TTCCGAAATA ACCGGTACCC CAATTTACAA CTGCGGGAGA TTGCTGGGCA TATAATGGAG
    TTTTCCCAAG ACCAGCATGG GTCCAGATTC ATTCAGCTGA AACTAGAACG AGCCACACCA
    GCTGAACGCC AGCTTGTCTT CAATGAAATC CTCCAGGCCG CTTACCAGCT AATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAAAAGTTC TTTGAATTTG GCAGCCTAGA ACAGAAGCTG
    GCTTTGGCAG AACGGATTCG AGGCCACGTC CTATCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAATTTATT CCCTCAGACC AGCAGGTAAT TAATGAGATG
    GTTCGGGAGC TAGACGGCCA CGTCCTGAAG TGTGTGAAAG ACCAGAATGG TAATCACGTG
    GTTCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTTTGC AGTTTATTAT TGATGCGTTT
    AAGGGGCAGG TATTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATC
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TATGTGATTC AGCACGTATT GGAGCATGGC
    CGTCCTGAGG ACAAAAGTAA AATTGTAGCA GAAATCCGAG GCAATGTGCT TGTATTGAGT
    CAGCACAAAT TTGCAAGCAA CGTTGTGGAG AAGTGTGTCA CTCATGCGTC CCGCACAGAG
    CGTGCTGTGC TTATCGACGA GGTATGCACC ATGAATGACG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGC GCAAGATCGT CATGCATAAG ATCCGGCCCC ACATTGCGAC TCTCCGCAAA
    TACACCTATG GCAAGCACAT TTTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGTGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965462.1
    CDS287..3868
    Translation

    Target ORF information:

    RefSeq Version XM_014109987.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGCCTCACCA ATGGCAGTGG GAGATACATC TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTGTT TTCTTCTTCT
    CGGTTGCGAT ATGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT
    TTCCGAAATA ACCGGTACCC CAATTTACAA CTGCGGGAGA TTGCTGGGCA TATAATGGAG
    TTTTCCCAAG ACCAGCATGG GTCCAGATTC ATTCAGCTGA AACTAGAACG AGCCACACCA
    GCTGAACGCC AGCTTGTCTT CAATGAAATC CTCCAGGCCG CTTACCAGCT AATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAAAAGTTC TTTGAATTTG GCAGCCTAGA ACAGAAGCTG
    GCTTTGGCAG AACGGATTCG AGGCCACGTC CTATCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAATTTATT CCCTCAGACC AGCAGGTAAT TAATGAGATG
    GTTCGGGAGC TAGACGGCCA CGTCCTGAAG TGTGTGAAAG ACCAGAATGG TAATCACGTG
    GTTCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTTTGC AGTTTATTAT TGATGCGTTT
    AAGGGGCAGG TATTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATC
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TATGTGATTC AGCACGTATT GGAGCATGGC
    CGTCCTGAGG ACAAAAGTAA AATTGTAGCA GAAATCCGAG GCAATGTGCT TGTATTGAGT
    CAGCACAAAT TTGCAAGCAA CGTTGTGGAG AAGTGTGTCA CTCATGCGTC CCGCACAGAG
    CGTGCTGTGC TTATCGACGA GGTATGCACC ATGAATGACG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGC GCAAGATCGT CATGCATAAG ATCCGGCCCC ACATTGCGAC TCTCCGCAAA
    TACACCTATG GCAAGCACAT TTTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGTGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03438
    Clone ID Related Accession (Same CDS sequence) XM_014109987.1 , XM_005617672.2 , XM_014109987.2
    Accession Version XM_005617672.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGCCTCACCA ATGGCAGTGG GAGATACATC TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTGTT TTCTTCTTCT
    CGGTTGCGAT ATGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT
    TTCCGAAATA ACCGGTACCC CAATTTACAA CTGCGGGAGA TTGCTGGGCA TATAATGGAG
    TTTTCCCAAG ACCAGCATGG GTCCAGATTC ATTCAGCTGA AACTAGAACG AGCCACACCA
    GCTGAACGCC AGCTTGTCTT CAATGAAATC CTCCAGGCCG CTTACCAGCT AATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAAAAGTTC TTTGAATTTG GCAGCCTAGA ACAGAAGCTG
    GCTTTGGCAG AACGGATTCG AGGCCACGTC CTATCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAATTTATT CCCTCAGACC AGCAGGTAAT TAATGAGATG
    GTTCGGGAGC TAGACGGCCA CGTCCTGAAG TGTGTGAAAG ACCAGAATGG TAATCACGTG
    GTTCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTTTGC AGTTTATTAT TGATGCGTTT
    AAGGGGCAGG TATTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATC
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TATGTGATTC AGCACGTATT GGAGCATGGC
    CGTCCTGAGG ACAAAAGTAA AATTGTAGCA GAAATCCGAG GCAATGTGCT TGTATTGAGT
    CAGCACAAAT TTGCAAGCAA CGTTGTGGAG AAGTGTGTCA CTCATGCGTC CCGCACAGAG
    CGTGCTGTGC TTATCGACGA GGTATGCACC ATGAATGACG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGC GCAAGATCGT CATGCATAAG ATCCGGCCCC ACATTGCGAC TCTCCGCAAA
    TACACCTATG GCAAGCACAT TTTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGTGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617729.1
    CDS287..3868
    Translation

    Target ORF information:

    RefSeq Version XM_005617672.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617672.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGCCTCACCA ATGGCAGTGG GAGATACATC TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTGTT TTCTTCTTCT
    CGGTTGCGAT ATGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT
    TTCCGAAATA ACCGGTACCC CAATTTACAA CTGCGGGAGA TTGCTGGGCA TATAATGGAG
    TTTTCCCAAG ACCAGCATGG GTCCAGATTC ATTCAGCTGA AACTAGAACG AGCCACACCA
    GCTGAACGCC AGCTTGTCTT CAATGAAATC CTCCAGGCCG CTTACCAGCT AATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAAAAGTTC TTTGAATTTG GCAGCCTAGA ACAGAAGCTG
    GCTTTGGCAG AACGGATTCG AGGCCACGTC CTATCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAATTTATT CCCTCAGACC AGCAGGTAAT TAATGAGATG
    GTTCGGGAGC TAGACGGCCA CGTCCTGAAG TGTGTGAAAG ACCAGAATGG TAATCACGTG
    GTTCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTTTGC AGTTTATTAT TGATGCGTTT
    AAGGGGCAGG TATTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATC
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TATGTGATTC AGCACGTATT GGAGCATGGC
    CGTCCTGAGG ACAAAAGTAA AATTGTAGCA GAAATCCGAG GCAATGTGCT TGTATTGAGT
    CAGCACAAAT TTGCAAGCAA CGTTGTGGAG AAGTGTGTCA CTCATGCGTC CCGCACAGAG
    CGTGCTGTGC TTATCGACGA GGTATGCACC ATGAATGACG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGC GCAAGATCGT CATGCATAAG ATCCGGCCCC ACATTGCGAC TCTCCGCAAA
    TACACCTATG GCAAGCACAT TTTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGTGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03438
    Clone ID Related Accession (Same CDS sequence) XM_014109987.1 , XM_005617672.2 , XM_014109987.2
    Accession Version XM_014109987.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pumilio homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGCCTCACCA ATGGCAGTGG GAGATACATC TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTGTT TTCTTCTTCT
    CGGTTGCGAT ATGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT
    TTCCGAAATA ACCGGTACCC CAATTTACAA CTGCGGGAGA TTGCTGGGCA TATAATGGAG
    TTTTCCCAAG ACCAGCATGG GTCCAGATTC ATTCAGCTGA AACTAGAACG AGCCACACCA
    GCTGAACGCC AGCTTGTCTT CAATGAAATC CTCCAGGCCG CTTACCAGCT AATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAAAAGTTC TTTGAATTTG GCAGCCTAGA ACAGAAGCTG
    GCTTTGGCAG AACGGATTCG AGGCCACGTC CTATCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAATTTATT CCCTCAGACC AGCAGGTAAT TAATGAGATG
    GTTCGGGAGC TAGACGGCCA CGTCCTGAAG TGTGTGAAAG ACCAGAATGG TAATCACGTG
    GTTCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTTTGC AGTTTATTAT TGATGCGTTT
    AAGGGGCAGG TATTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATC
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TATGTGATTC AGCACGTATT GGAGCATGGC
    CGTCCTGAGG ACAAAAGTAA AATTGTAGCA GAAATCCGAG GCAATGTGCT TGTATTGAGT
    CAGCACAAAT TTGCAAGCAA CGTTGTGGAG AAGTGTGTCA CTCATGCGTC CCGCACAGAG
    CGTGCTGTGC TTATCGACGA GGTATGCACC ATGAATGACG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGC GCAAGATCGT CATGCATAAG ATCCGGCCCC ACATTGCGAC TCTCCGCAAA
    TACACCTATG GCAAGCACAT TTTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGTGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965462.1
    CDS163..3744
    Translation

    Target ORF information:

    RefSeq Version XM_014109987.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pumilio RNA binding family member 1 (PUM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109987.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTGTGTTT
    TCAGCAGGTT TAGCGCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG TGCTGCTCCC
    CCGGGGACGG ACCCCTACAC AGCTGGACTG GCTGCAGCGG CGACACTAGG CCCGGCTGTG
    GTCCCTCACC AGTATTATGG AGTCACTCCC TGGGGAGTCT ACCCAGCCAG TCTCTTCCAG
    CAGCAAGCAG CTGCTGCTGC GGCAGCAACT AATTCTGCAA ATCAGCAGAC TACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACCCCA
    AATCAGAACC AGCAGGGACA GCAAACAGAT CCTCTGGTGG CAGCTGCAGC AGTGAATTCT
    GCCCTTGCTT TTGGACAAGG ACTGGCTGCA GGCATGCCAG GTTACCCGGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGCGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGCGCTCCTG TTCGGCTGGT AGCTCCTGCC CCAGTCATTA TCAGTTCCTC GGCTGCACAA
    GCAGCTGTTG CGGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACC CAGCAGCCCC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA GCCTGGCATC CAGCTCTTTC TATGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGCCTCACCA ATGGCAGTGG GAGATACATC TCTGCCGCGC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCGA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTGTT TTCTTCTTCT
    CGGTTGCGAT ATGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TCTGGAAGAT
    TTCCGAAATA ACCGGTACCC CAATTTACAA CTGCGGGAGA TTGCTGGGCA TATAATGGAG
    TTTTCCCAAG ACCAGCATGG GTCCAGATTC ATTCAGCTGA AACTAGAACG AGCCACACCA
    GCTGAACGCC AGCTTGTCTT CAATGAAATC CTCCAGGCCG CTTACCAGCT AATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAAAAGTTC TTTGAATTTG GCAGCCTAGA ACAGAAGCTG
    GCTTTGGCAG AACGGATTCG AGGCCACGTC CTATCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAATTTATT CCCTCAGACC AGCAGGTAAT TAATGAGATG
    GTTCGGGAGC TAGACGGCCA CGTCCTGAAG TGTGTGAAAG ACCAGAATGG TAATCACGTG
    GTTCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTTTGC AGTTTATTAT TGATGCGTTT
    AAGGGGCAGG TATTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATC
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TATGTGATTC AGCACGTATT GGAGCATGGC
    CGTCCTGAGG ACAAAAGTAA AATTGTAGCA GAAATCCGAG GCAATGTGCT TGTATTGAGT
    CAGCACAAAT TTGCAAGCAA CGTTGTGGAG AAGTGTGTCA CTCATGCGTC CCGCACAGAG
    CGTGCTGTGC TTATCGACGA GGTATGCACC ATGAATGACG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGC GCAAGATCGT CATGCATAAG ATCCGGCCCC ACATTGCGAC TCTCCGCAAA
    TACACCTATG GCAAGCACAT TTTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGTGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03439
    Clone ID Related Accession (Same CDS sequence) XM_014109994.1
    Accession Version XM_014109994.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTCTTGCC AGGTAAAAAG TTTTGGGAAA CAGATGAATC CAGCAAAGAT
    GGACCAAAAG GAATATTCCT GGGTGATCAA TGGCGAGACA GTGCCTGGGG AACATCAGAT
    CATTCAGTTT CCCAGCCAAT CATGGTGCAG AGAAGACCTG GTCAGAGTTT CCATGTGAAC
    AGTGAGGTCA ATTCTGTACT GTCCCCACGA TCGGAGAGTG GGGGACTAGG CGTTAGCATG
    GTGGAGTATG TGTTGAGCTC ATCCCCGGGC GATTCCTGTC TAAGAAAAGG AGGATTTGGC
    CCAAGGGATG CAGACAGTGA TGAAAACGAC AAAGGTGACA AGAAGAACAA GGGTGCGTTT
    GATGGAGATA AGCTAGGAGA TTTGAAGGAG GAGGGTGATG TGATGGACAA GACGAATGGT
    TTGCCAGTGC AGAATGGGAT TGATGCAGAC GTCAAAGATT TTAGCCGTAC CCCTGGTAAT
    TGCCAGAACT CTGCTAATGA AGTGGATCTT CTGGGTCCAA ACCAGAATGG TTCTGAGGGC
    TTAGCCCAGC TGACCAGCAC CAATGGTGCC AAGCCTGTGG AGGATTTCTC CAACATGGAG
    TCCCAGAGTG TCCCCTTGGA CCCCATGGAA CATGTGGGCA TGGAGCCTCT TCAGTTTGAT
    TATTCAGGCA CGCAGGTACC TGTGGACTCA GCAGCAGCAA CTGTGGGACT TTTTGACTAC
    AATTCTCAAC AACAGCTGTT CCAAAGACCT AATGCACTTG CTGTCCAGCA GTTGACAGCT
    GCCCAGCAGC AGCAGTATGC ACTGGCAGCG GCTCATCAAC CTCACATCGG TGTGTTTTCA
    GCAGGTTTAG CGCCCGCTGC GTTTGTCCCC AATCCATACA TCATCAGTGC TGCTCCCCCG
    GGGACGGACC CCTACACAGC TGGACTGGCT GCAGCGGCGA CACTAGGCCC GGCTGTGGTC
    CCTCACCAGT ATTATGGAGT CACTCCCTGG GGAGTCTACC CAGCCAGTCT CTTCCAGCAG
    CAAGCAGCTG CTGCTGCGGC AGCAACTAAT TCTGCAAATC AGCAGACTAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACCCCAAAT
    CAGAACCAGC AGGGACAGCA AACAGATCCT CTGGTGGCAG CTGCAGCAGT GAATTCTGCC
    CTTGCTTTTG GACAAGGACT GGCTGCAGGC ATGCCAGGTT ACCCGGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG CGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGC
    GCTCCTGTTC GGCTGGTAGC TCCTGCCCCA GTCATTATCA GTTCCTCGGC TGCACAAGCA
    GCTGTTGCGG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACCCAG CAGCCCCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAGCC TGGCATCCAG CTCTTTCTAT GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCTGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGC
    CTCACCAATG GCAGTGGGAG ATACATCTCT GCCGCGCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCGAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTGTTTTC TTCTTCTCGG
    TTGCGATATG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTCT GGAAGATTTC
    CGAAATAACC GGTACCCCAA TTTACAACTG CGGGAGATTG CTGGGCATAT AATGGAGTTT
    TCCCAAGACC AGCATGGGTC CAGATTCATT CAGCTGAAAC TAGAACGAGC CACACCAGCT
    GAACGCCAGC TTGTCTTCAA TGAAATCCTC CAGGCCGCTT ACCAGCTAAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA AAAGTTCTTT GAATTTGGCA GCCTAGAACA GAAGCTGGCT
    TTGGCAGAAC GGATTCGAGG CCACGTCCTA TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA ATTTATTCCC TCAGACCAGC AGGTAATTAA TGAGATGGTT
    CGGGAGCTAG ACGGCCACGT CCTGAAGTGT GTGAAAGACC AGAATGGTAA TCACGTGGTT
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTTTGCAGT TTATTATTGA TGCGTTTAAG
    GGGCAGGTAT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATCCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAT GTGATTCAGC ACGTATTGGA GCATGGCCGT
    CCTGAGGACA AAAGTAAAAT TGTAGCAGAA ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG
    CACAAATTTG CAAGCAACGT TGTGGAGAAG TGTGTCACTC ATGCGTCCCG CACAGAGCGT
    GCTGTGCTTA TCGACGAGGT ATGCACCATG AATGACGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGCGCA AGATCGTCAT GCATAAGATC CGGCCCCACA TTGCGACTCT CCGCAAATAC
    ACCTATGGCA AGCACATTTT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGTGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965469.1
    CDS287..3865
    Translation

    Target ORF information:

    RefSeq Version XM_014109994.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109994.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTCTTGCC AGGTAAAAAG TTTTGGGAAA CAGATGAATC CAGCAAAGAT
    GGACCAAAAG GAATATTCCT GGGTGATCAA TGGCGAGACA GTGCCTGGGG AACATCAGAT
    CATTCAGTTT CCCAGCCAAT CATGGTGCAG AGAAGACCTG GTCAGAGTTT CCATGTGAAC
    AGTGAGGTCA ATTCTGTACT GTCCCCACGA TCGGAGAGTG GGGGACTAGG CGTTAGCATG
    GTGGAGTATG TGTTGAGCTC ATCCCCGGGC GATTCCTGTC TAAGAAAAGG AGGATTTGGC
    CCAAGGGATG CAGACAGTGA TGAAAACGAC AAAGGTGACA AGAAGAACAA GGGTGCGTTT
    GATGGAGATA AGCTAGGAGA TTTGAAGGAG GAGGGTGATG TGATGGACAA GACGAATGGT
    TTGCCAGTGC AGAATGGGAT TGATGCAGAC GTCAAAGATT TTAGCCGTAC CCCTGGTAAT
    TGCCAGAACT CTGCTAATGA AGTGGATCTT CTGGGTCCAA ACCAGAATGG TTCTGAGGGC
    TTAGCCCAGC TGACCAGCAC CAATGGTGCC AAGCCTGTGG AGGATTTCTC CAACATGGAG
    TCCCAGAGTG TCCCCTTGGA CCCCATGGAA CATGTGGGCA TGGAGCCTCT TCAGTTTGAT
    TATTCAGGCA CGCAGGTACC TGTGGACTCA GCAGCAGCAA CTGTGGGACT TTTTGACTAC
    AATTCTCAAC AACAGCTGTT CCAAAGACCT AATGCACTTG CTGTCCAGCA GTTGACAGCT
    GCCCAGCAGC AGCAGTATGC ACTGGCAGCG GCTCATCAAC CTCACATCGG TGTGTTTTCA
    GCAGGTTTAG CGCCCGCTGC GTTTGTCCCC AATCCATACA TCATCAGTGC TGCTCCCCCG
    GGGACGGACC CCTACACAGC TGGACTGGCT GCAGCGGCGA CACTAGGCCC GGCTGTGGTC
    CCTCACCAGT ATTATGGAGT CACTCCCTGG GGAGTCTACC CAGCCAGTCT CTTCCAGCAG
    CAAGCAGCTG CTGCTGCGGC AGCAACTAAT TCTGCAAATC AGCAGACTAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACCCCAAAT
    CAGAACCAGC AGGGACAGCA AACAGATCCT CTGGTGGCAG CTGCAGCAGT GAATTCTGCC
    CTTGCTTTTG GACAAGGACT GGCTGCAGGC ATGCCAGGTT ACCCGGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG CGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGC
    GCTCCTGTTC GGCTGGTAGC TCCTGCCCCA GTCATTATCA GTTCCTCGGC TGCACAAGCA
    GCTGTTGCGG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACCCAG CAGCCCCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAGCC TGGCATCCAG CTCTTTCTAT GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCTGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGC
    CTCACCAATG GCAGTGGGAG ATACATCTCT GCCGCGCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCGAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTGTTTTC TTCTTCTCGG
    TTGCGATATG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTCT GGAAGATTTC
    CGAAATAACC GGTACCCCAA TTTACAACTG CGGGAGATTG CTGGGCATAT AATGGAGTTT
    TCCCAAGACC AGCATGGGTC CAGATTCATT CAGCTGAAAC TAGAACGAGC CACACCAGCT
    GAACGCCAGC TTGTCTTCAA TGAAATCCTC CAGGCCGCTT ACCAGCTAAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA AAAGTTCTTT GAATTTGGCA GCCTAGAACA GAAGCTGGCT
    TTGGCAGAAC GGATTCGAGG CCACGTCCTA TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA ATTTATTCCC TCAGACCAGC AGGTAATTAA TGAGATGGTT
    CGGGAGCTAG ACGGCCACGT CCTGAAGTGT GTGAAAGACC AGAATGGTAA TCACGTGGTT
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTTTGCAGT TTATTATTGA TGCGTTTAAG
    GGGCAGGTAT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATCCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAT GTGATTCAGC ACGTATTGGA GCATGGCCGT
    CCTGAGGACA AAAGTAAAAT TGTAGCAGAA ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG
    CACAAATTTG CAAGCAACGT TGTGGAGAAG TGTGTCACTC ATGCGTCCCG CACAGAGCGT
    GCTGTGCTTA TCGACGAGGT ATGCACCATG AATGACGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGCGCA AGATCGTCAT GCATAAGATC CGGCCCCACA TTGCGACTCT CCGCAAATAC
    ACCTATGGCA AGCACATTTT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGTGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03440
    Clone ID Related Accession (Same CDS sequence) XM_005617675.2
    Accession Version XM_005617675.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGCTGTTGCG
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACCCA GCAGCCCCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAGC
    CTGGCATCCA GCTCTTTCTA TGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCTGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG CCTCACCAAT
    GGCAGTGGGA GATACATCTC TGCCGCGCCA GGCGCTGAAG CCAAGTACCG CAGCGCGAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTGTTTT CTTCTTCTCG GTTGCGATAT
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTC TGGAAGATTT CCGAAATAAC
    CGGTACCCCA ATTTACAACT GCGGGAGATT GCTGGGCATA TAATGGAGTT TTCCCAAGAC
    CAGCATGGGT CCAGATTCAT TCAGCTGAAA CTAGAACGAG CCACACCAGC TGAACGCCAG
    CTTGTCTTCA ATGAAATCCT CCAGGCCGCT TACCAGCTAA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AAAAGTTCTT TGAATTTGGC AGCCTAGAAC AGAAGCTGGC TTTGGCAGAA
    CGGATTCGAG GCCACGTCCT ATCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AATTTATTCC CTCAGACCAG CAGGTAATTA ATGAGATGGT TCGGGAGCTA
    GACGGCCACG TCCTGAAGTG TGTGAAAGAC CAGAATGGTA ATCACGTGGT TCAGAAGTGC
    ATCGAATGTG TGCAGCCCCA GTCTTTGCAG TTTATTATTG ATGCGTTTAA GGGGCAGGTA
    TTTGCTTTGT CCACGCATCC TTATGGCTGC CGAGTGATTC AGAGGATCCT GGAGCACTGT
    CTCCCTGACC AGACACTCCC TATTTTAGAG GAGCTTCACC AGCACACAGA GCAACTTGTA
    CAGGATCAGT ATGGAAATTA TGTGATTCAG CACGTATTGG AGCATGGCCG TCCTGAGGAC
    AAAAGTAAAA TTGTAGCAGA AATCCGAGGC AATGTGCTTG TATTGAGTCA GCACAAATTT
    GCAAGCAACG TTGTGGAGAA GTGTGTCACT CATGCGTCCC GCACAGAGCG TGCTGTGCTT
    ATCGACGAGG TATGCACCAT GAATGACGGT CCCCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAACTACGT GGTCCAGAAG ATGATCGATG TGGCAGAGCC AGCCCAGCGC
    AAGATCGTCA TGCATAAGAT CCGGCCCCAC ATTGCGACTC TCCGCAAATA CACCTATGGC
    AAGCACATTT TGGCCAAGCT GGAGAAATAC TACATGAAGA ATGGCGTTGA CTTAGGGCCC
    ATCTGTGGCC CCCCTAATGG TATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617732.1
    CDS286..3855
    Translation

    Target ORF information:

    RefSeq Version XM_005617675.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617675.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGCAGGTTTA
    GCGCCCGCTG CGTTTGTCCC CAATCCATAC ATCATCAGTG CTGCTCCCCC GGGGACGGAC
    CCCTACACAG CTGGACTGGC TGCAGCGGCG ACACTAGGCC CGGCTGTGGT CCCTCACCAG
    TATTATGGAG TCACTCCCTG GGGAGTCTAC CCAGCCAGTC TCTTCCAGCA GCAAGCAGCT
    GCTGCTGCGG CAGCAACTAA TTCTGCAAAT CAGCAGACTA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACCCCAAA TCAGAACCAG
    CAGGGACAGC AAACAGATCC TCTGGTGGCA GCTGCAGCAG TGAATTCTGC CCTTGCTTTT
    GGACAAGGAC TGGCTGCAGG CATGCCAGGT TACCCGGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GCGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG CGCTCCTGTT
    CGGCTGGTAG CTCCTGCCCC AGTCATTATC AGTTCCTCGG CTGCACAAGC AGCTGTTGCG
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACCCA GCAGCCCCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAGC
    CTGGCATCCA GCTCTTTCTA TGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCTGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG CCTCACCAAT
    GGCAGTGGGA GATACATCTC TGCCGCGCCA GGCGCTGAAG CCAAGTACCG CAGCGCGAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTGTTTT CTTCTTCTCG GTTGCGATAT
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTC TGGAAGATTT CCGAAATAAC
    CGGTACCCCA ATTTACAACT GCGGGAGATT GCTGGGCATA TAATGGAGTT TTCCCAAGAC
    CAGCATGGGT CCAGATTCAT TCAGCTGAAA CTAGAACGAG CCACACCAGC TGAACGCCAG
    CTTGTCTTCA ATGAAATCCT CCAGGCCGCT TACCAGCTAA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AAAAGTTCTT TGAATTTGGC AGCCTAGAAC AGAAGCTGGC TTTGGCAGAA
    CGGATTCGAG GCCACGTCCT ATCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AATTTATTCC CTCAGACCAG CAGGTAATTA ATGAGATGGT TCGGGAGCTA
    GACGGCCACG TCCTGAAGTG TGTGAAAGAC CAGAATGGTA ATCACGTGGT TCAGAAGTGC
    ATCGAATGTG TGCAGCCCCA GTCTTTGCAG TTTATTATTG ATGCGTTTAA GGGGCAGGTA
    TTTGCTTTGT CCACGCATCC TTATGGCTGC CGAGTGATTC AGAGGATCCT GGAGCACTGT
    CTCCCTGACC AGACACTCCC TATTTTAGAG GAGCTTCACC AGCACACAGA GCAACTTGTA
    CAGGATCAGT ATGGAAATTA TGTGATTCAG CACGTATTGG AGCATGGCCG TCCTGAGGAC
    AAAAGTAAAA TTGTAGCAGA AATCCGAGGC AATGTGCTTG TATTGAGTCA GCACAAATTT
    GCAAGCAACG TTGTGGAGAA GTGTGTCACT CATGCGTCCC GCACAGAGCG TGCTGTGCTT
    ATCGACGAGG TATGCACCAT GAATGACGGT CCCCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAACTACGT GGTCCAGAAG ATGATCGATG TGGCAGAGCC AGCCCAGCGC
    AAGATCGTCA TGCATAAGAT CCGGCCCCAC ATTGCGACTC TCCGCAAATA CACCTATGGC
    AAGCACATTT TGGCCAAGCT GGAGAAATAC TACATGAAGA ATGGCGTTGA CTTAGGGCCC
    ATCTGTGGCC CCCCTAATGG TATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03441
    Clone ID Related Accession (Same CDS sequence) XM_005617677.2
    Accession Version XM_005617677.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTTTAGCG
    CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGTGCTG CTCCCCCGGG GACGGACCCC
    TACACAGCTG GACTGGCTGC AGCGGCGACA CTAGGCCCGG CTGTGGTCCC TCACCAGTAT
    TATGGAGTCA CTCCCTGGGG AGTCTACCCA GCCAGTCTCT TCCAGCAGCA AGCAGCTGCT
    GCTGCGGCAG CAACTAATTC TGCAAATCAG CAGACTACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CCCCAAATCA GAACCAGCAG
    GGACAGCAAA CAGATCCTCT GGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCTTTTGGA
    CAAGGACTGG CTGCAGGCAT GCCAGGTTAC CCGGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGCG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGCGC TCCTGTTCGG
    CTGGTAGCTC CTGCCCCAGT CATTATCAGT TCCTCGGCTG CACAAGCAGC TGTTGCGGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACCCAGCA GCCCCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAGCCTG
    GCATCCAGCT CTTTCTATGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGCCT CACCAATGGC
    AGTGGGAGAT ACATCTCTGC CGCGCCAGGC GCTGAAGCCA AGTACCGCAG CGCGAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTGTTTTCTT CTTCTCGGTT GCGATATGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTCTGG AAGATTTCCG AAATAACCGG
    TACCCCAATT TACAACTGCG GGAGATTGCT GGGCATATAA TGGAGTTTTC CCAAGACCAG
    CATGGGTCCA GATTCATTCA GCTGAAACTA GAACGAGCCA CACCAGCTGA ACGCCAGCTT
    GTCTTCAATG AAATCCTCCA GGCCGCTTAC CAGCTAATGG TGGATGTATT TGGAAATTAC
    GTCATTCAAA AGTTCTTTGA ATTTGGCAGC CTAGAACAGA AGCTGGCTTT GGCAGAACGG
    ATTCGAGGCC ACGTCCTATC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAAT TTATTCCCTC AGACCAGCAG GTAATTAATG AGATGGTTCG GGAGCTAGAC
    GGCCACGTCC TGAAGTGTGT GAAAGACCAG AATGGTAATC ACGTGGTTCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TTTGCAGTTT ATTATTGATG CGTTTAAGGG GCAGGTATTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATCCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTATGT GATTCAGCAC GTATTGGAGC ATGGCCGTCC TGAGGACAAA
    AGTAAAATTG TAGCAGAAAT CCGAGGCAAT GTGCTTGTAT TGAGTCAGCA CAAATTTGCA
    AGCAACGTTG TGGAGAAGTG TGTCACTCAT GCGTCCCGCA CAGAGCGTGC TGTGCTTATC
    GACGAGGTAT GCACCATGAA TGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGCGCAAG
    ATCGTCATGC ATAAGATCCG GCCCCACATT GCGACTCTCC GCAAATACAC CTATGGCAAG
    CACATTTTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGTGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617734.1
    CDS286..3852
    Translation

    Target ORF information:

    RefSeq Version XM_005617677.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617677.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTTTAGCG
    CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGTGCTG CTCCCCCGGG GACGGACCCC
    TACACAGCTG GACTGGCTGC AGCGGCGACA CTAGGCCCGG CTGTGGTCCC TCACCAGTAT
    TATGGAGTCA CTCCCTGGGG AGTCTACCCA GCCAGTCTCT TCCAGCAGCA AGCAGCTGCT
    GCTGCGGCAG CAACTAATTC TGCAAATCAG CAGACTACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CCCCAAATCA GAACCAGCAG
    GGACAGCAAA CAGATCCTCT GGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCTTTTGGA
    CAAGGACTGG CTGCAGGCAT GCCAGGTTAC CCGGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGCG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGCGC TCCTGTTCGG
    CTGGTAGCTC CTGCCCCAGT CATTATCAGT TCCTCGGCTG CACAAGCAGC TGTTGCGGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACCCAGCA GCCCCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAGCCTG
    GCATCCAGCT CTTTCTATGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGCCT CACCAATGGC
    AGTGGGAGAT ACATCTCTGC CGCGCCAGGC GCTGAAGCCA AGTACCGCAG CGCGAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTGTTTTCTT CTTCTCGGTT GCGATATGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTCTGG AAGATTTCCG AAATAACCGG
    TACCCCAATT TACAACTGCG GGAGATTGCT GGGCATATAA TGGAGTTTTC CCAAGACCAG
    CATGGGTCCA GATTCATTCA GCTGAAACTA GAACGAGCCA CACCAGCTGA ACGCCAGCTT
    GTCTTCAATG AAATCCTCCA GGCCGCTTAC CAGCTAATGG TGGATGTATT TGGAAATTAC
    GTCATTCAAA AGTTCTTTGA ATTTGGCAGC CTAGAACAGA AGCTGGCTTT GGCAGAACGG
    ATTCGAGGCC ACGTCCTATC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAAT TTATTCCCTC AGACCAGCAG GTAATTAATG AGATGGTTCG GGAGCTAGAC
    GGCCACGTCC TGAAGTGTGT GAAAGACCAG AATGGTAATC ACGTGGTTCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TTTGCAGTTT ATTATTGATG CGTTTAAGGG GCAGGTATTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATCCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTATGT GATTCAGCAC GTATTGGAGC ATGGCCGTCC TGAGGACAAA
    AGTAAAATTG TAGCAGAAAT CCGAGGCAAT GTGCTTGTAT TGAGTCAGCA CAAATTTGCA
    AGCAACGTTG TGGAGAAGTG TGTCACTCAT GCGTCCCGCA CAGAGCGTGC TGTGCTTATC
    GACGAGGTAT GCACCATGAA TGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGCGCAAG
    ATCGTCATGC ATAAGATCCG GCCCCACATT GCGACTCTCC GCAAATACAC CTATGGCAAG
    CACATTTTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGTGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03442
    Clone ID Related Accession (Same CDS sequence) XM_860226.4
    Accession Version XM_860226.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860226.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTTTAGCG
    CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGTGCTG CTCCCCCGGG GACGGACCCC
    TACACAGCTG GACTGGCTGC AGCGGCGACA CTAGGCCCGG CTGTGGTCCC TCACCAGTAT
    TATGGAGTCA CTCCCTGGGG AGTCTACCCA GCCAGTCTCT TCCAGCAGCA AGCAGCTGCT
    GCTGCGGCAG CAACTAATTC TGCAAATCAG CAGACTACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CCCCAAATCA GAACCAGCAG
    GGACAGCAAA CAGATCCTCT GGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCTTTTGGA
    CAAGGACTGG CTGCAGGCAT GCCAGGTTAC CCGGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGCG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGCGC TCCTGTTCGG
    CTGGTAGCTC CTGCCCCAGT CATTATCAGT TCCTCGGCTG CACAAGCAGC TGTTGCGGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACCCAGCA GCCCCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAGCCTG
    GCATCCAGCT CTTTCTATGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGCCT CACCAATGGC
    AGTGGGAGAT ACATCTCTGC CGCGCCAGGC GCTGAAGCCA AGTACCGCAG CGCGAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTGTTTTCTT CTTCTCGGTT GCGATATGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTCTGG AAGATTTCCG AAATAACCGG
    TACCCCAATT TACAACTGCG GGAGATTGCT GGGCATATAA TGGAGTTTTC CCAAGACCAG
    CATGGGTCCA GATTCATTCA GCTGAAACTA GAACGAGCCA CACCAGCTGA ACGCCAGCTT
    GTCTTCAATG AAATCCTCCA GGCCGCTTAC CAGCTAATGG TGGATGTATT TGGAAATTAC
    GTCATTCAAA AGTTCTTTGA ATTTGGCAGC CTAGAACAGA AGCTGGCTTT GGCAGAACGG
    ATTCGAGGCC ACGTCCTATC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAAT TTATTCCCTC AGACCAGCAG AATGAGATGG TTCGGGAGCT AGACGGCCAC
    GTCCTGAAGT GTGTGAAAGA CCAGAATGGT AATCACGTGG TTCAGAAGTG CATCGAATGT
    GTGCAGCCCC AGTCTTTGCA GTTTATTATT GATGCGTTTA AGGGGCAGGT ATTTGCTTTG
    TCCACGCATC CTTATGGCTG CCGAGTGATT CAGAGGATCC TGGAGCACTG TCTCCCTGAC
    CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAACTTGT ACAGGATCAG
    TATGGAAATT ATGTGATTCA GCACGTATTG GAGCATGGCC GTCCTGAGGA CAAAAGTAAA
    ATTGTAGCAG AAATCCGAGG CAATGTGCTT GTATTGAGTC AGCACAAATT TGCAAGCAAC
    GTTGTGGAGA AGTGTGTCAC TCATGCGTCC CGCACAGAGC GTGCTGTGCT TATCGACGAG
    GTATGCACCA TGAATGACGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAACTACG TGGTCCAGAA GATGATCGAT GTGGCAGAGC CAGCCCAGCG CAAGATCGTC
    ATGCATAAGA TCCGGCCCCA CATTGCGACT CTCCGCAAAT ACACCTATGG CAAGCACATT
    TTGGCCAAGC TGGAGAAATA CTACATGAAG AATGGCGTTG ACTTAGGGCC CATCTGTGGC
    CCCCCTAATG GTATTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_865319.1
    CDS287..3847
    Translation

    Target ORF information:

    RefSeq Version XM_860226.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_860226.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG ACAAGAAGAA CAAGGGTGCG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACGAAT
    GGTTTGCCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCAC TTGCTGTCCA GCAGTTGACA
    GCTGCCCAGC AGCAGCAGTA TGCACTGGCA GCGGCTCATC AACCTCACAT CGGTTTAGCG
    CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGTGCTG CTCCCCCGGG GACGGACCCC
    TACACAGCTG GACTGGCTGC AGCGGCGACA CTAGGCCCGG CTGTGGTCCC TCACCAGTAT
    TATGGAGTCA CTCCCTGGGG AGTCTACCCA GCCAGTCTCT TCCAGCAGCA AGCAGCTGCT
    GCTGCGGCAG CAACTAATTC TGCAAATCAG CAGACTACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CCCCAAATCA GAACCAGCAG
    GGACAGCAAA CAGATCCTCT GGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCTTTTGGA
    CAAGGACTGG CTGCAGGCAT GCCAGGTTAC CCGGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGCG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGCGC TCCTGTTCGG
    CTGGTAGCTC CTGCCCCAGT CATTATCAGT TCCTCGGCTG CACAAGCAGC TGTTGCGGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACCCAGCA GCCCCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAGCCTG
    GCATCCAGCT CTTTCTATGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGCCT CACCAATGGC
    AGTGGGAGAT ACATCTCTGC CGCGCCAGGC GCTGAAGCCA AGTACCGCAG CGCGAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTGTTTTCTT CTTCTCGGTT GCGATATGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTCTGG AAGATTTCCG AAATAACCGG
    TACCCCAATT TACAACTGCG GGAGATTGCT GGGCATATAA TGGAGTTTTC CCAAGACCAG
    CATGGGTCCA GATTCATTCA GCTGAAACTA GAACGAGCCA CACCAGCTGA ACGCCAGCTT
    GTCTTCAATG AAATCCTCCA GGCCGCTTAC CAGCTAATGG TGGATGTATT TGGAAATTAC
    GTCATTCAAA AGTTCTTTGA ATTTGGCAGC CTAGAACAGA AGCTGGCTTT GGCAGAACGG
    ATTCGAGGCC ACGTCCTATC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAAT TTATTCCCTC AGACCAGCAG AATGAGATGG TTCGGGAGCT AGACGGCCAC
    GTCCTGAAGT GTGTGAAAGA CCAGAATGGT AATCACGTGG TTCAGAAGTG CATCGAATGT
    GTGCAGCCCC AGTCTTTGCA GTTTATTATT GATGCGTTTA AGGGGCAGGT ATTTGCTTTG
    TCCACGCATC CTTATGGCTG CCGAGTGATT CAGAGGATCC TGGAGCACTG TCTCCCTGAC
    CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAACTTGT ACAGGATCAG
    TATGGAAATT ATGTGATTCA GCACGTATTG GAGCATGGCC GTCCTGAGGA CAAAAGTAAA
    ATTGTAGCAG AAATCCGAGG CAATGTGCTT GTATTGAGTC AGCACAAATT TGCAAGCAAC
    GTTGTGGAGA AGTGTGTCAC TCATGCGTCC CGCACAGAGC GTGCTGTGCT TATCGACGAG
    GTATGCACCA TGAATGACGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAACTACG TGGTCCAGAA GATGATCGAT GTGGCAGAGC CAGCCCAGCG CAAGATCGTC
    ATGCATAAGA TCCGGCCCCA CATTGCGACT CTCCGCAAAT ACACCTATGG CAAGCACATT
    TTGGCCAAGC TGGAGAAATA CTACATGAAG AATGGCGTTG ACTTAGGGCC CATCTGTGGC
    CCCCCTAATG GTATTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03444
    Clone ID Related Accession (Same CDS sequence) XM_005617679.2 , XM_014110015.1
    Accession Version XM_005617679.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGACAAGAAG
    AACAAGGGTG CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACGA ATGGTTTGCC AGTGCAGAAT GGGATTGATG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGCTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCTAATGC ACTTGCTGTC
    CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG TATGCACTGG CAGCGGCTCA TCAACCTCAC
    ATCGGTGTGT TTTCAGCAGG TTTAGCGCCC GCTGCGTTTG TCCCCAATCC ATACATCATC
    AGTGCTGCTC CCCCGGGGAC GGACCCCTAC ACAGCTGGAC TGGCTGCAGC GGCGACACTA
    GGCCCGGCTG TGGTCCCTCA CCAGTATTAT GGAGTCACTC CCTGGGGAGT CTACCCAGCC
    AGTCTCTTCC AGCAGCAAGC AGCTGCTGCT GCGGCAGCAA CTAATTCTGC AAATCAGCAG
    ACTACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACCC CAAATCAGAA CCAGCAGGGA CAGCAAACAG ATCCTCTGGT GGCAGCTGCA
    GCAGTGAATT CTGCCCTTGC TTTTGGACAA GGACTGGCTG CAGGCATGCC AGGTTACCCG
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGCGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGCGCTCC TGTTCGGCTG GTAGCTCCTG CCCCAGTCAT TATCAGTTCC
    TCGGCTGCAC AAGCAGCTGT TGCGGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CCCAGCAGCC CCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAGCCTGGCA TCCAGCTCTT TCTATGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CTGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGCCTCAC CAATGGCAGT GGGAGATACA TCTCTGCCGC GCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC GAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTG
    TTTTCTTCTT CTCGGTTGCG ATATGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTCTGGAAG ATTTCCGAAA TAACCGGTAC CCCAATTTAC AACTGCGGGA GATTGCTGGG
    CATATAATGG AGTTTTCCCA AGACCAGCAT GGGTCCAGAT TCATTCAGCT GAAACTAGAA
    CGAGCCACAC CAGCTGAACG CCAGCTTGTC TTCAATGAAA TCCTCCAGGC CGCTTACCAG
    CTAATGGTGG ATGTATTTGG AAATTACGTC ATTCAAAAGT TCTTTGAATT TGGCAGCCTA
    GAACAGAAGC TGGCTTTGGC AGAACGGATT CGAGGCCACG TCCTATCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAATTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAGA TGGTTCGGGA GCTAGACGGC CACGTCCTGA AGTGTGTGAA AGACCAGAAT
    GGTAATCACG TGGTTCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTTT GCAGTTTATT
    ATTGATGCGT TTAAGGGGCA GGTATTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TCCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTATGTGAT TCAGCACGTA
    TTGGAGCATG GCCGTCCTGA GGACAAAAGT AAAATTGTAG CAGAAATCCG AGGCAATGTG
    CTTGTATTGA GTCAGCACAA ATTTGCAAGC AACGTTGTGG AGAAGTGTGT CACTCATGCG
    TCCCGCACAG AGCGTGCTGT GCTTATCGAC GAGGTATGCA CCATGAATGA CGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GCGCAAGATC GTCATGCATA AGATCCGGCC CCACATTGCG
    ACTCTCCGCA AATACACCTA TGGCAAGCAC ATTTTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGT GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617736.1
    CDS286..3579
    Translation

    Target ORF information:

    RefSeq Version XM_005617679.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617679.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGACAAGAAG
    AACAAGGGTG CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACGA ATGGTTTGCC AGTGCAGAAT GGGATTGATG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGCTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCTAATGC ACTTGCTGTC
    CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG TATGCACTGG CAGCGGCTCA TCAACCTCAC
    ATCGGTGTGT TTTCAGCAGG TTTAGCGCCC GCTGCGTTTG TCCCCAATCC ATACATCATC
    AGTGCTGCTC CCCCGGGGAC GGACCCCTAC ACAGCTGGAC TGGCTGCAGC GGCGACACTA
    GGCCCGGCTG TGGTCCCTCA CCAGTATTAT GGAGTCACTC CCTGGGGAGT CTACCCAGCC
    AGTCTCTTCC AGCAGCAAGC AGCTGCTGCT GCGGCAGCAA CTAATTCTGC AAATCAGCAG
    ACTACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACCC CAAATCAGAA CCAGCAGGGA CAGCAAACAG ATCCTCTGGT GGCAGCTGCA
    GCAGTGAATT CTGCCCTTGC TTTTGGACAA GGACTGGCTG CAGGCATGCC AGGTTACCCG
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGCGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGCGCTCC TGTTCGGCTG GTAGCTCCTG CCCCAGTCAT TATCAGTTCC
    TCGGCTGCAC AAGCAGCTGT TGCGGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CCCAGCAGCC CCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAGCCTGGCA TCCAGCTCTT TCTATGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CTGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGCCTCAC CAATGGCAGT GGGAGATACA TCTCTGCCGC GCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC GAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTG
    TTTTCTTCTT CTCGGTTGCG ATATGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTCTGGAAG ATTTCCGAAA TAACCGGTAC CCCAATTTAC AACTGCGGGA GATTGCTGGG
    CATATAATGG AGTTTTCCCA AGACCAGCAT GGGTCCAGAT TCATTCAGCT GAAACTAGAA
    CGAGCCACAC CAGCTGAACG CCAGCTTGTC TTCAATGAAA TCCTCCAGGC CGCTTACCAG
    CTAATGGTGG ATGTATTTGG AAATTACGTC ATTCAAAAGT TCTTTGAATT TGGCAGCCTA
    GAACAGAAGC TGGCTTTGGC AGAACGGATT CGAGGCCACG TCCTATCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAATTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAGA TGGTTCGGGA GCTAGACGGC CACGTCCTGA AGTGTGTGAA AGACCAGAAT
    GGTAATCACG TGGTTCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTTT GCAGTTTATT
    ATTGATGCGT TTAAGGGGCA GGTATTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TCCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTATGTGAT TCAGCACGTA
    TTGGAGCATG GCCGTCCTGA GGACAAAAGT AAAATTGTAG CAGAAATCCG AGGCAATGTG
    CTTGTATTGA GTCAGCACAA ATTTGCAAGC AACGTTGTGG AGAAGTGTGT CACTCATGCG
    TCCCGCACAG AGCGTGCTGT GCTTATCGAC GAGGTATGCA CCATGAATGA CGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GCGCAAGATC GTCATGCATA AGATCCGGCC CCACATTGCG
    ACTCTCCGCA AATACACCTA TGGCAAGCAC ATTTTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGT GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03444
    Clone ID Related Accession (Same CDS sequence) XM_005617679.2 , XM_014110015.1
    Accession Version XM_014110015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGACAAGAAG
    AACAAGGGTG CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACGA ATGGTTTGCC AGTGCAGAAT GGGATTGATG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGCTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCTAATGC ACTTGCTGTC
    CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG TATGCACTGG CAGCGGCTCA TCAACCTCAC
    ATCGGTGTGT TTTCAGCAGG TTTAGCGCCC GCTGCGTTTG TCCCCAATCC ATACATCATC
    AGTGCTGCTC CCCCGGGGAC GGACCCCTAC ACAGCTGGAC TGGCTGCAGC GGCGACACTA
    GGCCCGGCTG TGGTCCCTCA CCAGTATTAT GGAGTCACTC CCTGGGGAGT CTACCCAGCC
    AGTCTCTTCC AGCAGCAAGC AGCTGCTGCT GCGGCAGCAA CTAATTCTGC AAATCAGCAG
    ACTACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACCC CAAATCAGAA CCAGCAGGGA CAGCAAACAG ATCCTCTGGT GGCAGCTGCA
    GCAGTGAATT CTGCCCTTGC TTTTGGACAA GGACTGGCTG CAGGCATGCC AGGTTACCCG
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGCGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGCGCTCC TGTTCGGCTG GTAGCTCCTG CCCCAGTCAT TATCAGTTCC
    TCGGCTGCAC AAGCAGCTGT TGCGGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CCCAGCAGCC CCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAGCCTGGCA TCCAGCTCTT TCTATGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CTGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGCCTCAC CAATGGCAGT GGGAGATACA TCTCTGCCGC GCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC GAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTG
    TTTTCTTCTT CTCGGTTGCG ATATGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTCTGGAAG ATTTCCGAAA TAACCGGTAC CCCAATTTAC AACTGCGGGA GATTGCTGGG
    CATATAATGG AGTTTTCCCA AGACCAGCAT GGGTCCAGAT TCATTCAGCT GAAACTAGAA
    CGAGCCACAC CAGCTGAACG CCAGCTTGTC TTCAATGAAA TCCTCCAGGC CGCTTACCAG
    CTAATGGTGG ATGTATTTGG AAATTACGTC ATTCAAAAGT TCTTTGAATT TGGCAGCCTA
    GAACAGAAGC TGGCTTTGGC AGAACGGATT CGAGGCCACG TCCTATCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAATTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAGA TGGTTCGGGA GCTAGACGGC CACGTCCTGA AGTGTGTGAA AGACCAGAAT
    GGTAATCACG TGGTTCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTTT GCAGTTTATT
    ATTGATGCGT TTAAGGGGCA GGTATTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TCCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTATGTGAT TCAGCACGTA
    TTGGAGCATG GCCGTCCTGA GGACAAAAGT AAAATTGTAG CAGAAATCCG AGGCAATGTG
    CTTGTATTGA GTCAGCACAA ATTTGCAAGC AACGTTGTGG AGAAGTGTGT CACTCATGCG
    TCCCGCACAG AGCGTGCTGT GCTTATCGAC GAGGTATGCA CCATGAATGA CGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GCGCAAGATC GTCATGCATA AGATCCGGCC CCACATTGCG
    ACTCTCCGCA AATACACCTA TGGCAAGCAC ATTTTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGT GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965490.1
    CDS286..3579
    Translation

    Target ORF information:

    RefSeq Version XM_014110015.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGACAAGAAG
    AACAAGGGTG CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACGA ATGGTTTGCC AGTGCAGAAT GGGATTGATG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGCTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCTAATGC ACTTGCTGTC
    CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG TATGCACTGG CAGCGGCTCA TCAACCTCAC
    ATCGGTGTGT TTTCAGCAGG TTTAGCGCCC GCTGCGTTTG TCCCCAATCC ATACATCATC
    AGTGCTGCTC CCCCGGGGAC GGACCCCTAC ACAGCTGGAC TGGCTGCAGC GGCGACACTA
    GGCCCGGCTG TGGTCCCTCA CCAGTATTAT GGAGTCACTC CCTGGGGAGT CTACCCAGCC
    AGTCTCTTCC AGCAGCAAGC AGCTGCTGCT GCGGCAGCAA CTAATTCTGC AAATCAGCAG
    ACTACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACCC CAAATCAGAA CCAGCAGGGA CAGCAAACAG ATCCTCTGGT GGCAGCTGCA
    GCAGTGAATT CTGCCCTTGC TTTTGGACAA GGACTGGCTG CAGGCATGCC AGGTTACCCG
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGCGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGCGCTCC TGTTCGGCTG GTAGCTCCTG CCCCAGTCAT TATCAGTTCC
    TCGGCTGCAC AAGCAGCTGT TGCGGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CCCAGCAGCC CCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAGCCTGGCA TCCAGCTCTT TCTATGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CTGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGCCTCAC CAATGGCAGT GGGAGATACA TCTCTGCCGC GCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC GAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTG
    TTTTCTTCTT CTCGGTTGCG ATATGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTCTGGAAG ATTTCCGAAA TAACCGGTAC CCCAATTTAC AACTGCGGGA GATTGCTGGG
    CATATAATGG AGTTTTCCCA AGACCAGCAT GGGTCCAGAT TCATTCAGCT GAAACTAGAA
    CGAGCCACAC CAGCTGAACG CCAGCTTGTC TTCAATGAAA TCCTCCAGGC CGCTTACCAG
    CTAATGGTGG ATGTATTTGG AAATTACGTC ATTCAAAAGT TCTTTGAATT TGGCAGCCTA
    GAACAGAAGC TGGCTTTGGC AGAACGGATT CGAGGCCACG TCCTATCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAATTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAGA TGGTTCGGGA GCTAGACGGC CACGTCCTGA AGTGTGTGAA AGACCAGAAT
    GGTAATCACG TGGTTCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTTT GCAGTTTATT
    ATTGATGCGT TTAAGGGGCA GGTATTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TCCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTATGTGAT TCAGCACGTA
    TTGGAGCATG GCCGTCCTGA GGACAAAAGT AAAATTGTAG CAGAAATCCG AGGCAATGTG
    CTTGTATTGA GTCAGCACAA ATTTGCAAGC AACGTTGTGG AGAAGTGTGT CACTCATGCG
    TCCCGCACAG AGCGTGCTGT GCTTATCGAC GAGGTATGCA CCATGAATGA CGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GCGCAAGATC GTCATGCATA AGATCCGGCC CCACATTGCG
    ACTCTCCGCA AATACACCTA TGGCAAGCAC ATTTTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGT GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03445
    Clone ID Related Accession (Same CDS sequence) XM_005617680.2
    Accession Version XM_005617680.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pumilio homolog 1 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGACAAGAAG
    AACAAGGGTG CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACGA ATGGTTTGCC AGTGCAGAAT GGGATTGATG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGCTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCTAATGC ACTTGCTGTC
    CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG TATGCACTGG CAGCGGCTCA TCAACCTCAC
    ATCGGTTTAG CGCCCGCTGC GTTTGTCCCC AATCCATACA TCATCAGTGC TGCTCCCCCG
    GGGACGGACC CCTACACAGC TGGACTGGCT GCAGCGGCGA CACTAGGCCC GGCTGTGGTC
    CCTCACCAGT ATTATGGAGT CACTCCCTGG GGAGTCTACC CAGCCAGTCT CTTCCAGCAG
    CAAGCAGCTG CTGCTGCGGC AGCAACTAAT TCTGCAAATC AGCAGACTAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACCCCAAAT
    CAGAACCAGC AGGGACAGCA AACAGATCCT CTGGTGGCAG CTGCAGCAGT GAATTCTGCC
    CTTGCTTTTG GACAAGGACT GGCTGCAGGC ATGCCAGGTT ACCCGGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG CGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGC
    GCTCCTGTTC GGCTGGTAGC TCCTGCCCCA GTCATTATCA GTTCCTCGGC TGCACAAGCA
    GCTGTTGCGG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACCCAG CAGCCCCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAGCC TGGCATCCAG CTCTTTCTAT GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCTGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGC
    CTCACCAATG GCAGTGGGAG ATACATCTCT GCCGCGCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCGAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTGTTTTC TTCTTCTCGG
    TTGCGATATG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTCT GGAAGATTTC
    CGAAATAACC GGTACCCCAA TTTACAACTG CGGGAGATTG CTGGGCATAT AATGGAGTTT
    TCCCAAGACC AGCATGGGTC CAGATTCATT CAGCTGAAAC TAGAACGAGC CACACCAGCT
    GAACGCCAGC TTGTCTTCAA TGAAATCCTC CAGGCCGCTT ACCAGCTAAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA AAAGTTCTTT GAATTTGGCA GCCTAGAACA GAAGCTGGCT
    TTGGCAGAAC GGATTCGAGG CCACGTCCTA TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA ATTTATTCCC TCAGACCAGC AGGTAATTAA TGAGATGGTT
    CGGGAGCTAG ACGGCCACGT CCTGAAGTGT GTGAAAGACC AGAATGGTAA TCACGTGGTT
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTTTGCAGT TTATTATTGA TGCGTTTAAG
    GGGCAGGTAT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATCCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAT GTGATTCAGC ACGTATTGGA GCATGGCCGT
    CCTGAGGACA AAAGTAAAAT TGTAGCAGAA ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG
    CACAAATTTG CAAGCAACGT TGTGGAGAAG TGTGTCACTC ATGCGTCCCG CACAGAGCGT
    GCTGTGCTTA TCGACGAGGT ATGCACCATG AATGACGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGCGCA AGATCGTCAT GCATAAGATC CGGCCCCACA TTGCGACTCT CCGCAAATAC
    ACCTATGGCA AGCACATTTT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGTGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617737.1
    CDS286..3564
    Translation

    Target ORF information:

    RefSeq Version XM_005617680.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pumilio RNA-binding family member 1 (PUM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617680.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGAC GATCCCAAGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG CAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA GCTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGACAAGAAG
    AACAAGGGTG CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACGA ATGGTTTGCC AGTGCAGAAT GGGATTGATG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGCTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCTAATGC ACTTGCTGTC
    CAGCAGTTGA CAGCTGCCCA GCAGCAGCAG TATGCACTGG CAGCGGCTCA TCAACCTCAC
    ATCGGTTTAG CGCCCGCTGC GTTTGTCCCC AATCCATACA TCATCAGTGC TGCTCCCCCG
    GGGACGGACC CCTACACAGC TGGACTGGCT GCAGCGGCGA CACTAGGCCC GGCTGTGGTC
    CCTCACCAGT ATTATGGAGT CACTCCCTGG GGAGTCTACC CAGCCAGTCT CTTCCAGCAG
    CAAGCAGCTG CTGCTGCGGC AGCAACTAAT TCTGCAAATC AGCAGACTAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACCCCAAAT
    CAGAACCAGC AGGGACAGCA AACAGATCCT CTGGTGGCAG CTGCAGCAGT GAATTCTGCC
    CTTGCTTTTG GACAAGGACT GGCTGCAGGC ATGCCAGGTT ACCCGGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG CGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGC
    GCTCCTGTTC GGCTGGTAGC TCCTGCCCCA GTCATTATCA GTTCCTCGGC TGCACAAGCA
    GCTGTTGCGG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACCCAG CAGCCCCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAGCC TGGCATCCAG CTCTTTCTAT GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCTGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGC
    CTCACCAATG GCAGTGGGAG ATACATCTCT GCCGCGCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCGAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTGTTTTC TTCTTCTCGG
    TTGCGATATG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTCT GGAAGATTTC
    CGAAATAACC GGTACCCCAA TTTACAACTG CGGGAGATTG CTGGGCATAT AATGGAGTTT
    TCCCAAGACC AGCATGGGTC CAGATTCATT CAGCTGAAAC TAGAACGAGC CACACCAGCT
    GAACGCCAGC TTGTCTTCAA TGAAATCCTC CAGGCCGCTT ACCAGCTAAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA AAAGTTCTTT GAATTTGGCA GCCTAGAACA GAAGCTGGCT
    TTGGCAGAAC GGATTCGAGG CCACGTCCTA TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA ATTTATTCCC TCAGACCAGC AGGTAATTAA TGAGATGGTT
    CGGGAGCTAG ACGGCCACGT CCTGAAGTGT GTGAAAGACC AGAATGGTAA TCACGTGGTT
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTTTGCAGT TTATTATTGA TGCGTTTAAG
    GGGCAGGTAT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATCCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAT GTGATTCAGC ACGTATTGGA GCATGGCCGT
    CCTGAGGACA AAAGTAAAAT TGTAGCAGAA ATCCGAGGCA ATGTGCTTGT ATTGAGTCAG
    CACAAATTTG CAAGCAACGT TGTGGAGAAG TGTGTCACTC ATGCGTCCCG CACAGAGCGT
    GCTGTGCTTA TCGACGAGGT ATGCACCATG AATGACGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGCGCA AGATCGTCAT GCATAAGATC CGGCCCCACA TTGCGACTCT CCGCAAATAC
    ACCTATGGCA AGCACATTTT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGTGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.