PUM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PUM1
Entrez Gene ID	9698
Full Name	pumilio RNA binding family member 1
Synonyms	HSPUM,PUMH,PUMH1,PUML1
General protein information	Preferred Names pumilio RNA binding family member 1 Names pumilio homolog 1 pumilio-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p35.2
Summary	This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008].
Disorder MIM:	607204

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020658.2	NP_001018494.1	pumilio homolog 1 isoform 1
NM_014676.2	NP_055491.1	pumilio homolog 1 isoform 2
NM_001020658.1	NP_001018494.1	pumilio homolog 1 isoform 1
NM_014676.3	NP_055491.1	pumilio homolog 1 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pum1	NP_001153076.1
Rattus norvegicus (Norway rat)	Pum1	NP_001102154.1
Canis lupus familiaris (dog)	PUM1	XP_005617729.1
Gallus gallus (chicken)	PUM1	NP_001012858.1
Xenopus tropicalis (tropical clawed frog)	pum1	XP_002939403.2
Homo sapiens (human)	PUM1	NP_001018494.1
Pan troglodytes (chimpanzee)	PUM1	XP_001157067.1
	PUM1	XP_001096289.1
Bos taurus (cattle)	PUM1	NP_001180052.1
Danio rerio (zebrafish)	pum1	NP_001264048.1

Gene Ontology

The following PUM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04252	NM_001020658.2 Latest version!	Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu06617	NM_014676.3 Latest version!	Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
View Old Accession Versions >>
OHu04252	NM_001020658.1	Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu06617	NM_014676.2	Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PUM1 ( NM_001020658.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018494.1 Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04252
Clone ID Related Accession (Same CDS sequence)	NM_001020658.2 , NM_001020658.1
Accession Version	NM_001020658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	pumilio homolog 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL699026.1, AK222605.1, AF315592.1 and CN402209.1. On Jun 2, 2019 this sequence version replaced NM_001020658.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.71919.1, SRR1803614.259856.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT 
GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACT 
CACTCCAGCC CTGTCCCAGG ATCTATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG 
GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGAGGA 
GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT 
CAGGTGCGTT CCATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA 
GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA 
GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG 
AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC 
ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT 
GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG 
TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT 
GGTTTACCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT 
AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG 
GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG 
GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT 
GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC 
TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCGC TTGCTGTCCA GCAGTTGACA 
GCTGCTCAGC AGCAGCAGTA TGCACTGGCA GCTGCTCATC AGCCGCACAT CGGTTTAGCT 
CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGCGCTG CTCCCCCAGG GACGGACCCC 
TACACAGCTG GATTGGCTGC AGCAGCGACA CTAGGCCCAG CTGTGGTCCC TCACCAGTAT 
TATGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCTGCCGCT 
GCCGCTGCAG CAACTAATTC AGCTAATCAA CAGACCACCC CACAGGCTCA GCAAGGACAG 
CAGCAGGTTC TCCGTGGAGG AGCCAGCCAA CGTCCTTTGA CCCCAAACCA GAACCAGCAG 
GGACAGCAAA CGGATCCCCT TGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCATTTGGA 
CAAGGTCTGG CAGCAGGCAT GCCAGGTTAT CCGGTGTTGG CTCCTGCTGC TTACTATGAC 
CAAACTGGTG CCCTTGTAGT GAATGCAGGC GCGAGAAATG GTCTTGGAGC TCCTGTTCGA 
CTTGTAGCTC CTGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCA 
GCCGCAGCTT CAGCAAATGG AGCAGCTGGT GGTCTTGCTG GAACAACAAA TGGACCATTT 
CGCCCTTTAG GAACACAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAA TAACAACCTG 
GCATCCAGTT CTTTCTACGG CAACAACTCT CTGAACAGCA ATTCACAGAG CAGCTCCCTC 
TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTCGGAAG TAGCAGTTCT 
CTCGGCGCCA CCCTGGGATC CGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC 
ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA 
TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT 
CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT 
CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACGAATGGC 
AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG TGCAAGCAGC 
GCCTCCAGCC TCTTCAGCCC GAGCAGCACT CTTTTCTCTT CCTCTCGTTT GCGATATGGA 
ATGTCTGATG TCATGCCTTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAACAACCGG 
TACCCCAATT TACAACTGCG GGAGATTGCT GGACATATAA TGGAATTTTC CCAAGACCAG 
CATGGGTCCA GATTCATTCA GCTGAAACTG GAGCGTGCCA CACCAGCTGA GCGCCAGCTT 
GTCTTCAATG AAATCCTCCA GGCTGCCTAC CAACTCATGG TGGATGTGTT TGGTAATTAC 
GTCATTCAGA AGTTCTTTGA ATTTGGCAGT CTTGAACAGA AGCTGGCTTT GGCAGAACGG 
ATTCGAGGCC ACGTCCTGTC ATTGGCACTA CAGATGTATG GCTGCCGTGT TATCCAGAAA 
GCTCTTGAGT TTATTCCTTC AGACCAGCAG GTAATTAATG AGATGGTTCG GGAACTAGAT 
GGCCATGTCT TGAAGTGTGT GAAAGATCAG AATGGCAATC ACGTGGTTCA GAAATGCATT 
GAATGTGTAC AGCCCCAGTC TTTGCAATTT ATCATCGATG CGTTTAAGGG ACAGGTATTT 
GCCTTATCCA CACATCCTTA TGGCTGCCGA GTGATTCAGA GAATCCTGGA GCACTGTCTC 
CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA GCTTGTACAG 
GATCAATATG GAAATTATGT AATCCAACAT GTACTGGAGC ACGGTCGTCC TGAGGATAAA 
AGCAAAATTG TAGCAGAAAT CCGAGGCAAT GTACTTGTAT TGAGTCAGCA CAAATTTGCA 
AGCAATGTTG TGGAGAAGTG TGTTACTCAC GCCTCACGTA CGGAGCGCGC TGTGCTCATC 
GATGAGGTGT GCACCATGAA CGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC 
CAGTATGCCA ACTACGTGGT CCAGAAGATG ATTGACGTGG CGGAGCCAGG CCAGCGGAAG 
ATCGTCATGC ATAAGATCCG GCCCCACATC GCAACTCTTC GTAAGTACAC CTATGGCAAG 
CACATTCTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GTGTTGACTT AGGGCCCATC 
TGTGGCCCCC CTAATGGTAT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018494.1
CDS	114..3680
Translation	MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGEQLLPGKKFWETDESSKDGPKGIFLGDQWRDSAWGTSDHSVSQPIMVQRRPGQSFHVNSEVNSVLSPRSESGGLGVSMVEYVLSSSPGDSCLRKGGFGPRDADSDENDKGEKKNKGTFDGDKLGDLKEEGDVMDKTNGLPVQNGIDADVKDFSRTPGNCQNSANEVDLLGPNQNGSEGLAQLTSTNGAKPVEDFSNMESQSVPLDPMEHVGMEPLQFDYSGTQVPVDSAAATVGLFDYNSQQQLFQRPNALAVQQLTAAQQQQYALAAAHQPHIGLAPAAFVPNPYIISAAPPGTDPYTAGLAAAATLGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSANQQTTPQAQQGQQQVLRGGASQRPLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYDQTGALVVNAGARNGLGAPVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQQPQPQPQQQPNNNLASSSFYGNNSLNSNSQSSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTAVANSNTGSGSRRDSLTGSSDLYKRTSSSLTPIGHSFYNGLSFSSSPGPVGMPLPSQGPGHSQTPPPSLSSHGSSSSLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFSSSRLRYGMSDVMPSGRSRLLEDFRNNRYPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATPAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGSLEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQVINEMVRELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAVLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPGQRKIVMHKIRPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGII

Target ORF information:

RefSeq Version	NM_001020658.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020658.2

ORF Insert Sequence:

ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT 
GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACT 
CACTCCAGCC CTGTCCCAGG ATCTATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG 
GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGAGGA 
GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT 
CAGGTGCGTT CCATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA 
GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA 
GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG 
AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC 
ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT 
GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG 
TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT 
GGTTTACCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT 
AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG 
GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG 
GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT 
GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC 
TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCGC TTGCTGTCCA GCAGTTGACA 
GCTGCTCAGC AGCAGCAGTA TGCACTGGCA GCTGCTCATC AGCCGCACAT CGGTTTAGCT 
CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGCGCTG CTCCCCCAGG GACGGACCCC 
TACACAGCTG GATTGGCTGC AGCAGCGACA CTAGGCCCAG CTGTGGTCCC TCACCAGTAT 
TATGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCTGCCGCT 
GCCGCTGCAG CAACTAATTC AGCTAATCAA CAGACCACCC CACAGGCTCA GCAAGGACAG 
CAGCAGGTTC TCCGTGGAGG AGCCAGCCAA CGTCCTTTGA CCCCAAACCA GAACCAGCAG 
GGACAGCAAA CGGATCCCCT TGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCATTTGGA 
CAAGGTCTGG CAGCAGGCAT GCCAGGTTAT CCGGTGTTGG CTCCTGCTGC TTACTATGAC 
CAAACTGGTG CCCTTGTAGT GAATGCAGGC GCGAGAAATG GTCTTGGAGC TCCTGTTCGA 
CTTGTAGCTC CTGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCA 
GCCGCAGCTT CAGCAAATGG AGCAGCTGGT GGTCTTGCTG GAACAACAAA TGGACCATTT 
CGCCCTTTAG GAACACAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAA TAACAACCTG 
GCATCCAGTT CTTTCTACGG CAACAACTCT CTGAACAGCA ATTCACAGAG CAGCTCCCTC 
TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTCGGAAG TAGCAGTTCT 
CTCGGCGCCA CCCTGGGATC CGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC 
ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA 
TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT 
CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT 
CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACGAATGGC 
AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG TGCAAGCAGC 
GCCTCCAGCC TCTTCAGCCC GAGCAGCACT CTTTTCTCTT CCTCTCGTTT GCGATATGGA 
ATGTCTGATG TCATGCCTTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAACAACCGG 
TACCCCAATT TACAACTGCG GGAGATTGCT GGACATATAA TGGAATTTTC CCAAGACCAG 
CATGGGTCCA GATTCATTCA GCTGAAACTG GAGCGTGCCA CACCAGCTGA GCGCCAGCTT 
GTCTTCAATG AAATCCTCCA GGCTGCCTAC CAACTCATGG TGGATGTGTT TGGTAATTAC 
GTCATTCAGA AGTTCTTTGA ATTTGGCAGT CTTGAACAGA AGCTGGCTTT GGCAGAACGG 
ATTCGAGGCC ACGTCCTGTC ATTGGCACTA CAGATGTATG GCTGCCGTGT TATCCAGAAA 
GCTCTTGAGT TTATTCCTTC AGACCAGCAG GTAATTAATG AGATGGTTCG GGAACTAGAT 
GGCCATGTCT TGAAGTGTGT GAAAGATCAG AATGGCAATC ACGTGGTTCA GAAATGCATT 
GAATGTGTAC AGCCCCAGTC TTTGCAATTT ATCATCGATG CGTTTAAGGG ACAGGTATTT 
GCCTTATCCA CACATCCTTA TGGCTGCCGA GTGATTCAGA GAATCCTGGA GCACTGTCTC 
CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA GCTTGTACAG 
GATCAATATG GAAATTATGT AATCCAACAT GTACTGGAGC ACGGTCGTCC TGAGGATAAA 
AGCAAAATTG TAGCAGAAAT CCGAGGCAAT GTACTTGTAT TGAGTCAGCA CAAATTTGCA 
AGCAATGTTG TGGAGAAGTG TGTTACTCAC GCCTCACGTA CGGAGCGCGC TGTGCTCATC 
GATGAGGTGT GCACCATGAA CGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC 
CAGTATGCCA ACTACGTGGT CCAGAAGATG ATTGACGTGG CGGAGCCAGG CCAGCGGAAG 
ATCGTCATGC ATAAGATCCG GCCCCACATC GCAACTCTTC GTAAGTACAC CTATGGCAAG 
CACATTCTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GTGTTGACTT AGGGCCCATC 
TGTGGCCCCC CTAATGGTAT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUM1 ( NM_001020658.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018494.1 Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04252
Clone ID Related Accession (Same CDS sequence)	NM_001020658.2 , NM_001020658.1
Accession Version	NM_001020658.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	pumilio homolog 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL699026.1, AK222605.1, AF315592.1 and CN402209.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.259856.1, SRR1660809.219553.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT 
GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACT 
CACTCCAGCC CTGTCCCAGG ATCTATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG 
GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGAGGA 
GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT 
CAGGTGCGTT CCATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA 
GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA 
GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG 
AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC 
ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT 
GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG 
TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT 
GGTTTACCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT 
AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG 
GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG 
GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT 
GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC 
TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCGC TTGCTGTCCA GCAGTTGACA 
GCTGCTCAGC AGCAGCAGTA TGCACTGGCA GCTGCTCATC AGCCGCACAT CGGTTTAGCT 
CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGCGCTG CTCCCCCAGG GACGGACCCC 
TACACAGCTG GATTGGCTGC AGCAGCGACA CTAGGCCCAG CTGTGGTCCC TCACCAGTAT 
TATGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCTGCCGCT 
GCCGCTGCAG CAACTAATTC AGCTAATCAA CAGACCACCC CACAGGCTCA GCAAGGACAG 
CAGCAGGTTC TCCGTGGAGG AGCCAGCCAA CGTCCTTTGA CCCCAAACCA GAACCAGCAG 
GGACAGCAAA CGGATCCCCT TGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCATTTGGA 
CAAGGTCTGG CAGCAGGCAT GCCAGGTTAT CCGGTGTTGG CTCCTGCTGC TTACTATGAC 
CAAACTGGTG CCCTTGTAGT GAATGCAGGC GCGAGAAATG GTCTTGGAGC TCCTGTTCGA 
CTTGTAGCTC CTGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCA 
GCCGCAGCTT CAGCAAATGG AGCAGCTGGT GGTCTTGCTG GAACAACAAA TGGACCATTT 
CGCCCTTTAG GAACACAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAA TAACAACCTG 
GCATCCAGTT CTTTCTACGG CAACAACTCT CTGAACAGCA ATTCACAGAG CAGCTCCCTC 
TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTCGGAAG TAGCAGTTCT 
CTCGGCGCCA CCCTGGGATC CGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC 
ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA 
TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT 
CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT 
CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACGAATGGC 
AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG TGCAAGCAGC 
GCCTCCAGCC TCTTCAGCCC GAGCAGCACT CTTTTCTCTT CCTCTCGTTT GCGATATGGA 
ATGTCTGATG TCATGCCTTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAACAACCGG 
TACCCCAATT TACAACTGCG GGAGATTGCT GGACATATAA TGGAATTTTC CCAAGACCAG 
CATGGGTCCA GATTCATTCA GCTGAAACTG GAGCGTGCCA CACCAGCTGA GCGCCAGCTT 
GTCTTCAATG AAATCCTCCA GGCTGCCTAC CAACTCATGG TGGATGTGTT TGGTAATTAC 
GTCATTCAGA AGTTCTTTGA ATTTGGCAGT CTTGAACAGA AGCTGGCTTT GGCAGAACGG 
ATTCGAGGCC ACGTCCTGTC ATTGGCACTA CAGATGTATG GCTGCCGTGT TATCCAGAAA 
GCTCTTGAGT TTATTCCTTC AGACCAGCAG GTAATTAATG AGATGGTTCG GGAACTAGAT 
GGCCATGTCT TGAAGTGTGT GAAAGATCAG AATGGCAATC ACGTGGTTCA GAAATGCATT 
GAATGTGTAC AGCCCCAGTC TTTGCAATTT ATCATCGATG CGTTTAAGGG ACAGGTATTT 
GCCTTATCCA CACATCCTTA TGGCTGCCGA GTGATTCAGA GAATCCTGGA GCACTGTCTC 
CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA GCTTGTACAG 
GATCAATATG GAAATTATGT AATCCAACAT GTACTGGAGC ACGGTCGTCC TGAGGATAAA 
AGCAAAATTG TAGCAGAAAT CCGAGGCAAT GTACTTGTAT TGAGTCAGCA CAAATTTGCA 
AGCAATGTTG TGGAGAAGTG TGTTACTCAC GCCTCACGTA CGGAGCGCGC TGTGCTCATC 
GATGAGGTGT GCACCATGAA CGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC 
CAGTATGCCA ACTACGTGGT CCAGAAGATG ATTGACGTGG CGGAGCCAGG CCAGCGGAAG 
ATCGTCATGC ATAAGATCCG GCCCCACATC GCAACTCTTC GTAAGTACAC CTATGGCAAG 
CACATTCTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GTGTTGACTT AGGGCCCATC 
TGTGGCCCCC CTAATGGTAT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018494.1
CDS	114..3680
Translation	MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGEQLLPGKKFWETDESSKDGPKGIFLGDQWRDSAWGTSDHSVSQPIMVQRRPGQSFHVNSEVNSVLSPRSESGGLGVSMVEYVLSSSPGDSCLRKGGFGPRDADSDENDKGEKKNKGTFDGDKLGDLKEEGDVMDKTNGLPVQNGIDADVKDFSRTPGNCQNSANEVDLLGPNQNGSEGLAQLTSTNGAKPVEDFSNMESQSVPLDPMEHVGMEPLQFDYSGTQVPVDSAAATVGLFDYNSQQQLFQRPNALAVQQLTAAQQQQYALAAAHQPHIGLAPAAFVPNPYIISAAPPGTDPYTAGLAAAATLGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSANQQTTPQAQQGQQQVLRGGASQRPLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYDQTGALVVNAGARNGLGAPVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQQPQPQPQQQPNNNLASSSFYGNNSLNSNSQSSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTAVANSNTGSGSRRDSLTGSSDLYKRTSSSLTPIGHSFYNGLSFSSSPGPVGMPLPSQGPGHSQTPPPSLSSHGSSSSLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFSSSRLRYGMSDVMPSGRSRLLEDFRNNRYPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATPAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGSLEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQVINEMVRELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAVLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPGQRKIVMHKIRPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGII

Target ORF information:

RefSeq Version	NM_001020658.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020658.1

ORF Insert Sequence:

ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT 
GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACT 
CACTCCAGCC CTGTCCCAGG ATCTATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG 
GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGAGGA 
GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT 
CAGGTGCGTT CCATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA 
GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA 
GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG 
AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC 
ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT 
GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG 
TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT 
GGTTTACCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT 
AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG 
GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG 
GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT 
GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC 
TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCGC TTGCTGTCCA GCAGTTGACA 
GCTGCTCAGC AGCAGCAGTA TGCACTGGCA GCTGCTCATC AGCCGCACAT CGGTTTAGCT 
CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGCGCTG CTCCCCCAGG GACGGACCCC 
TACACAGCTG GATTGGCTGC AGCAGCGACA CTAGGCCCAG CTGTGGTCCC TCACCAGTAT 
TATGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCTGCCGCT 
GCCGCTGCAG CAACTAATTC AGCTAATCAA CAGACCACCC CACAGGCTCA GCAAGGACAG 
CAGCAGGTTC TCCGTGGAGG AGCCAGCCAA CGTCCTTTGA CCCCAAACCA GAACCAGCAG 
GGACAGCAAA CGGATCCCCT TGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCATTTGGA 
CAAGGTCTGG CAGCAGGCAT GCCAGGTTAT CCGGTGTTGG CTCCTGCTGC TTACTATGAC 
CAAACTGGTG CCCTTGTAGT GAATGCAGGC GCGAGAAATG GTCTTGGAGC TCCTGTTCGA 
CTTGTAGCTC CTGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCA 
GCCGCAGCTT CAGCAAATGG AGCAGCTGGT GGTCTTGCTG GAACAACAAA TGGACCATTT 
CGCCCTTTAG GAACACAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAA TAACAACCTG 
GCATCCAGTT CTTTCTACGG CAACAACTCT CTGAACAGCA ATTCACAGAG CAGCTCCCTC 
TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTCGGAAG TAGCAGTTCT 
CTCGGCGCCA CCCTGGGATC CGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC 
ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA 
TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT 
CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT 
CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACGAATGGC 
AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG TGCAAGCAGC 
GCCTCCAGCC TCTTCAGCCC GAGCAGCACT CTTTTCTCTT CCTCTCGTTT GCGATATGGA 
ATGTCTGATG TCATGCCTTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAACAACCGG 
TACCCCAATT TACAACTGCG GGAGATTGCT GGACATATAA TGGAATTTTC CCAAGACCAG 
CATGGGTCCA GATTCATTCA GCTGAAACTG GAGCGTGCCA CACCAGCTGA GCGCCAGCTT 
GTCTTCAATG AAATCCTCCA GGCTGCCTAC CAACTCATGG TGGATGTGTT TGGTAATTAC 
GTCATTCAGA AGTTCTTTGA ATTTGGCAGT CTTGAACAGA AGCTGGCTTT GGCAGAACGG 
ATTCGAGGCC ACGTCCTGTC ATTGGCACTA CAGATGTATG GCTGCCGTGT TATCCAGAAA 
GCTCTTGAGT TTATTCCTTC AGACCAGCAG GTAATTAATG AGATGGTTCG GGAACTAGAT 
GGCCATGTCT TGAAGTGTGT GAAAGATCAG AATGGCAATC ACGTGGTTCA GAAATGCATT 
GAATGTGTAC AGCCCCAGTC TTTGCAATTT ATCATCGATG CGTTTAAGGG ACAGGTATTT 
GCCTTATCCA CACATCCTTA TGGCTGCCGA GTGATTCAGA GAATCCTGGA GCACTGTCTC 
CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA GCTTGTACAG 
GATCAATATG GAAATTATGT AATCCAACAT GTACTGGAGC ACGGTCGTCC TGAGGATAAA 
AGCAAAATTG TAGCAGAAAT CCGAGGCAAT GTACTTGTAT TGAGTCAGCA CAAATTTGCA 
AGCAATGTTG TGGAGAAGTG TGTTACTCAC GCCTCACGTA CGGAGCGCGC TGTGCTCATC 
GATGAGGTGT GCACCATGAA CGACGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC 
CAGTATGCCA ACTACGTGGT CCAGAAGATG ATTGACGTGG CGGAGCCAGG CCAGCGGAAG 
ATCGTCATGC ATAAGATCCG GCCCCACATC GCAACTCTTC GTAAGTACAC CTATGGCAAG 
CACATTCTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GTGTTGACTT AGGGCCCATC 
TGTGGCCCCC CTAATGGTAT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUM1 ( NM_014676.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055491.1 Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 2, mRNA.

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CloneID	OHu06617
Clone ID Related Accession (Same CDS sequence)	NM_014676.2 , NM_014676.3
Accession Version	NM_014676.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	pumilio homolog 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL699026.1, AF315592.1 and CN402209.1. On Jun 3, 2005 this sequence version replaced NM_014676.1. Transcript Variant: This variant (2) uses an alternate, in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF315592.1, SRR1803613.136622.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT 
GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACT 
CACTCCAGCC CTGTCCCAGG ATCTATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG 
GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGAGGA 
GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT 
CAGGTGCGTT CCATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA 
GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA 
GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG 
AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC 
ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT 
GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG 
TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT 
GGTTTACCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT 
AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG 
GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG 
GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT 
GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC 
TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCGC TTGCTGTCCA GCAGTTGACA 
GCTGCTCAGC AGCAGCAGTA TGCACTGGCA GCTGCTCATC AGCCGCACAT CGGTTTAGCT 
CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGCGCTG CTCCCCCAGG GACGGACCCC 
TACACAGCTG GATTGGCTGC AGCAGCGACA CTAGGCCCAG CTGTGGTCCC TCACCAGTAT 
TATGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCTGCCGCT 
GCCGCTGCAG CAACTAATTC AGCTAATCAA CAGACCACCC CACAGGCTCA GCAAGGACAG 
CAGCAGGTTC TCCGTGGAGG AGCCAGCCAA CGTCCTTTGA CCCCAAACCA GAACCAGCAG 
GGACAGCAAA CGGATCCCCT TGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCATTTGGA 
CAAGGTCTGG CAGCAGGCAT GCCAGGTTAT CCGGTGTTGG CTCCTGCTGC TTACTATGAC 
CAAACTGGTG CCCTTGTAGT GAATGCAGGC GCGAGAAATG GTCTTGGAGC TCCTGTTCGA 
CTTGTAGCTC CTGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCA 
GCCGCAGCTT CAGCAAATGG AGCAGCTGGT GGTCTTGCTG GAACAACAAA TGGACCATTT 
CGCCCTTTAG GAACACAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAA TAACAACCTG 
GCATCCAGTT CTTTCTACGG CAACAACTCT CTGAACAGCA ATTCACAGAG CAGCTCCCTC 
TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTCGGAAG TAGCAGTTCT 
CTCGGCGCCA CCCTGGGATC CGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC 
ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA 
TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT 
CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT 
CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACGAATGGC 
AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG TGCAAGCAGC 
GCCTCCAGCC TCTTCAGCCC GAGCAGCACT CTTTTCTCTT CCTCTCGTTT GCGATATGGA 
ATGTCTGATG TCATGCCTTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAACAACCGG 
TACCCCAATT TACAACTGCG GGAGATTGCT GGACATATAA TGGAATTTTC CCAAGACCAG 
CATGGGTCCA GATTCATTCA GCTGAAACTG GAGCGTGCCA CACCAGCTGA GCGCCAGCTT 
GTCTTCAATG AAATCCTCCA GGCTGCCTAC CAACTCATGG TGGATGTGTT TGGTAATTAC 
GTCATTCAGA AGTTCTTTGA ATTTGGCAGT CTTGAACAGA AGCTGGCTTT GGCAGAACGG 
ATTCGAGGCC ACGTCCTGTC ATTGGCACTA CAGATGTATG GCTGCCGTGT TATCCAGAAA 
GCTCTTGAGT TTATTCCTTC AGACCAGCAG AATGAGATGG TTCGGGAACT AGATGGCCAT 
GTCTTGAAGT GTGTGAAAGA TCAGAATGGC AATCACGTGG TTCAGAAATG CATTGAATGT 
GTACAGCCCC AGTCTTTGCA ATTTATCATC GATGCGTTTA AGGGACAGGT ATTTGCCTTA 
TCCACACATC CTTATGGCTG CCGAGTGATT CAGAGAATCC TGGAGCACTG TCTCCCTGAC 
CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAGCTTGT ACAGGATCAA 
TATGGAAATT ATGTAATCCA ACATGTACTG GAGCACGGTC GTCCTGAGGA TAAAAGCAAA 
ATTGTAGCAG AAATCCGAGG CAATGTACTT GTATTGAGTC AGCACAAATT TGCAAGCAAT 
GTTGTGGAGA AGTGTGTTAC TCACGCCTCA CGTACGGAGC GCGCTGTGCT CATCGATGAG 
GTGTGCACCA TGAACGACGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT 
GCCAACTACG TGGTCCAGAA GATGATTGAC GTGGCGGAGC CAGGCCAGCG GAAGATCGTC 
ATGCATAAGA TCCGGCCCCA CATCGCAACT CTTCGTAAGT ACACCTATGG CAAGCACATT 
CTGGCCAAGC TGGAGAAGTA CTACATGAAG AACGGTGTTG ACTTAGGGCC CATCTGTGGC 
CCCCCTAATG GTATCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055491.1
CDS	114..3674
Translation	MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGEQLLPGKKFWETDESSKDGPKGIFLGDQWRDSAWGTSDHSVSQPIMVQRRPGQSFHVNSEVNSVLSPRSESGGLGVSMVEYVLSSSPGDSCLRKGGFGPRDADSDENDKGEKKNKGTFDGDKLGDLKEEGDVMDKTNGLPVQNGIDADVKDFSRTPGNCQNSANEVDLLGPNQNGSEGLAQLTSTNGAKPVEDFSNMESQSVPLDPMEHVGMEPLQFDYSGTQVPVDSAAATVGLFDYNSQQQLFQRPNALAVQQLTAAQQQQYALAAAHQPHIGLAPAAFVPNPYIISAAPPGTDPYTAGLAAAATLGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSANQQTTPQAQQGQQQVLRGGASQRPLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYDQTGALVVNAGARNGLGAPVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQQPQPQPQQQPNNNLASSSFYGNNSLNSNSQSSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTAVANSNTGSGSRRDSLTGSSDLYKRTSSSLTPIGHSFYNGLSFSSSPGPVGMPLPSQGPGHSQTPPPSLSSHGSSSSLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFSSSRLRYGMSDVMPSGRSRLLEDFRNNRYPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATPAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGSLEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQNEMVRELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAVLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPGQRKIVMHKIRPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGII

Target ORF information:

RefSeq Version	NM_014676.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014676.2

ORF Insert Sequence:

ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT 
GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACT 
CACTCCAGCC CTGTCCCAGG ATCTATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG 
GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGAGGA 
GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT 
CAGGTGCGTT CCATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA 
GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA 
GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG 
AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC 
ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT 
GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG 
TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT 
GGTTTACCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT 
AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG 
GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG 
GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT 
GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC 
TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCGC TTGCTGTCCA GCAGTTGACA 
GCTGCTCAGC AGCAGCAGTA TGCACTGGCA GCTGCTCATC AGCCGCACAT CGGTTTAGCT 
CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGCGCTG CTCCCCCAGG GACGGACCCC 
TACACAGCTG GATTGGCTGC AGCAGCGACA CTAGGCCCAG CTGTGGTCCC TCACCAGTAT 
TATGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCTGCCGCT 
GCCGCTGCAG CAACTAATTC AGCTAATCAA CAGACCACCC CACAGGCTCA GCAAGGACAG 
CAGCAGGTTC TCCGTGGAGG AGCCAGCCAA CGTCCTTTGA CCCCAAACCA GAACCAGCAG 
GGACAGCAAA CGGATCCCCT TGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCATTTGGA 
CAAGGTCTGG CAGCAGGCAT GCCAGGTTAT CCGGTGTTGG CTCCTGCTGC TTACTATGAC 
CAAACTGGTG CCCTTGTAGT GAATGCAGGC GCGAGAAATG GTCTTGGAGC TCCTGTTCGA 
CTTGTAGCTC CTGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCA 
GCCGCAGCTT CAGCAAATGG AGCAGCTGGT GGTCTTGCTG GAACAACAAA TGGACCATTT 
CGCCCTTTAG GAACACAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAA TAACAACCTG 
GCATCCAGTT CTTTCTACGG CAACAACTCT CTGAACAGCA ATTCACAGAG CAGCTCCCTC 
TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTCGGAAG TAGCAGTTCT 
CTCGGCGCCA CCCTGGGATC CGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC 
ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA 
TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT 
CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT 
CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACGAATGGC 
AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG TGCAAGCAGC 
GCCTCCAGCC TCTTCAGCCC GAGCAGCACT CTTTTCTCTT CCTCTCGTTT GCGATATGGA 
ATGTCTGATG TCATGCCTTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAACAACCGG 
TACCCCAATT TACAACTGCG GGAGATTGCT GGACATATAA TGGAATTTTC CCAAGACCAG 
CATGGGTCCA GATTCATTCA GCTGAAACTG GAGCGTGCCA CACCAGCTGA GCGCCAGCTT 
GTCTTCAATG AAATCCTCCA GGCTGCCTAC CAACTCATGG TGGATGTGTT TGGTAATTAC 
GTCATTCAGA AGTTCTTTGA ATTTGGCAGT CTTGAACAGA AGCTGGCTTT GGCAGAACGG 
ATTCGAGGCC ACGTCCTGTC ATTGGCACTA CAGATGTATG GCTGCCGTGT TATCCAGAAA 
GCTCTTGAGT TTATTCCTTC AGACCAGCAG AATGAGATGG TTCGGGAACT AGATGGCCAT 
GTCTTGAAGT GTGTGAAAGA TCAGAATGGC AATCACGTGG TTCAGAAATG CATTGAATGT 
GTACAGCCCC AGTCTTTGCA ATTTATCATC GATGCGTTTA AGGGACAGGT ATTTGCCTTA 
TCCACACATC CTTATGGCTG CCGAGTGATT CAGAGAATCC TGGAGCACTG TCTCCCTGAC 
CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAGCTTGT ACAGGATCAA 
TATGGAAATT ATGTAATCCA ACATGTACTG GAGCACGGTC GTCCTGAGGA TAAAAGCAAA 
ATTGTAGCAG AAATCCGAGG CAATGTACTT GTATTGAGTC AGCACAAATT TGCAAGCAAT 
GTTGTGGAGA AGTGTGTTAC TCACGCCTCA CGTACGGAGC GCGCTGTGCT CATCGATGAG 
GTGTGCACCA TGAACGACGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT 
GCCAACTACG TGGTCCAGAA GATGATTGAC GTGGCGGAGC CAGGCCAGCG GAAGATCGTC 
ATGCATAAGA TCCGGCCCCA CATCGCAACT CTTCGTAAGT ACACCTATGG CAAGCACATT 
CTGGCCAAGC TGGAGAAGTA CTACATGAAG AACGGTGTTG ACTTAGGGCC CATCTGTGGC 
CCCCCTAATG GTATCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUM1 ( NM_014676.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055491.1 Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 2, mRNA.

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CloneID	OHu06617
Clone ID Related Accession (Same CDS sequence)	NM_014676.2 , NM_014676.3
Accession Version	NM_014676.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	pumilio homolog 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL699026.1, AF315592.1 and CN402209.1. On Jun 2, 2019 this sequence version replaced NM_014676.2. Transcript Variant: This variant (2) uses an alternate, in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF315592.1, SRR1803611.126517.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT 
GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACT 
CACTCCAGCC CTGTCCCAGG ATCTATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG 
GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGAGGA 
GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT 
CAGGTGCGTT CCATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA 
GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA 
GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG 
AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC 
ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT 
GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG 
TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT 
GGTTTACCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT 
AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG 
GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG 
GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT 
GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC 
TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCGC TTGCTGTCCA GCAGTTGACA 
GCTGCTCAGC AGCAGCAGTA TGCACTGGCA GCTGCTCATC AGCCGCACAT CGGTTTAGCT 
CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGCGCTG CTCCCCCAGG GACGGACCCC 
TACACAGCTG GATTGGCTGC AGCAGCGACA CTAGGCCCAG CTGTGGTCCC TCACCAGTAT 
TATGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCTGCCGCT 
GCCGCTGCAG CAACTAATTC AGCTAATCAA CAGACCACCC CACAGGCTCA GCAAGGACAG 
CAGCAGGTTC TCCGTGGAGG AGCCAGCCAA CGTCCTTTGA CCCCAAACCA GAACCAGCAG 
GGACAGCAAA CGGATCCCCT TGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCATTTGGA 
CAAGGTCTGG CAGCAGGCAT GCCAGGTTAT CCGGTGTTGG CTCCTGCTGC TTACTATGAC 
CAAACTGGTG CCCTTGTAGT GAATGCAGGC GCGAGAAATG GTCTTGGAGC TCCTGTTCGA 
CTTGTAGCTC CTGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCA 
GCCGCAGCTT CAGCAAATGG AGCAGCTGGT GGTCTTGCTG GAACAACAAA TGGACCATTT 
CGCCCTTTAG GAACACAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAA TAACAACCTG 
GCATCCAGTT CTTTCTACGG CAACAACTCT CTGAACAGCA ATTCACAGAG CAGCTCCCTC 
TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTCGGAAG TAGCAGTTCT 
CTCGGCGCCA CCCTGGGATC CGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC 
ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA 
TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT 
CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT 
CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACGAATGGC 
AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG TGCAAGCAGC 
GCCTCCAGCC TCTTCAGCCC GAGCAGCACT CTTTTCTCTT CCTCTCGTTT GCGATATGGA 
ATGTCTGATG TCATGCCTTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAACAACCGG 
TACCCCAATT TACAACTGCG GGAGATTGCT GGACATATAA TGGAATTTTC CCAAGACCAG 
CATGGGTCCA GATTCATTCA GCTGAAACTG GAGCGTGCCA CACCAGCTGA GCGCCAGCTT 
GTCTTCAATG AAATCCTCCA GGCTGCCTAC CAACTCATGG TGGATGTGTT TGGTAATTAC 
GTCATTCAGA AGTTCTTTGA ATTTGGCAGT CTTGAACAGA AGCTGGCTTT GGCAGAACGG 
ATTCGAGGCC ACGTCCTGTC ATTGGCACTA CAGATGTATG GCTGCCGTGT TATCCAGAAA 
GCTCTTGAGT TTATTCCTTC AGACCAGCAG AATGAGATGG TTCGGGAACT AGATGGCCAT 
GTCTTGAAGT GTGTGAAAGA TCAGAATGGC AATCACGTGG TTCAGAAATG CATTGAATGT 
GTACAGCCCC AGTCTTTGCA ATTTATCATC GATGCGTTTA AGGGACAGGT ATTTGCCTTA 
TCCACACATC CTTATGGCTG CCGAGTGATT CAGAGAATCC TGGAGCACTG TCTCCCTGAC 
CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAGCTTGT ACAGGATCAA 
TATGGAAATT ATGTAATCCA ACATGTACTG GAGCACGGTC GTCCTGAGGA TAAAAGCAAA 
ATTGTAGCAG AAATCCGAGG CAATGTACTT GTATTGAGTC AGCACAAATT TGCAAGCAAT 
GTTGTGGAGA AGTGTGTTAC TCACGCCTCA CGTACGGAGC GCGCTGTGCT CATCGATGAG 
GTGTGCACCA TGAACGACGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT 
GCCAACTACG TGGTCCAGAA GATGATTGAC GTGGCGGAGC CAGGCCAGCG GAAGATCGTC 
ATGCATAAGA TCCGGCCCCA CATCGCAACT CTTCGTAAGT ACACCTATGG CAAGCACATT 
CTGGCCAAGC TGGAGAAGTA CTACATGAAG AACGGTGTTG ACTTAGGGCC CATCTGTGGC 
CCCCCTAATG GTATCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055491.1
CDS	114..3674
Translation	MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGEQLLPGKKFWETDESSKDGPKGIFLGDQWRDSAWGTSDHSVSQPIMVQRRPGQSFHVNSEVNSVLSPRSESGGLGVSMVEYVLSSSPGDSCLRKGGFGPRDADSDENDKGEKKNKGTFDGDKLGDLKEEGDVMDKTNGLPVQNGIDADVKDFSRTPGNCQNSANEVDLLGPNQNGSEGLAQLTSTNGAKPVEDFSNMESQSVPLDPMEHVGMEPLQFDYSGTQVPVDSAAATVGLFDYNSQQQLFQRPNALAVQQLTAAQQQQYALAAAHQPHIGLAPAAFVPNPYIISAAPPGTDPYTAGLAAAATLGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSANQQTTPQAQQGQQQVLRGGASQRPLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYDQTGALVVNAGARNGLGAPVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQQPQPQPQQQPNNNLASSSFYGNNSLNSNSQSSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTAVANSNTGSGSRRDSLTGSSDLYKRTSSSLTPIGHSFYNGLSFSSSPGPVGMPLPSQGPGHSQTPPPSLSSHGSSSSLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFSSSRLRYGMSDVMPSGRSRLLEDFRNNRYPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATPAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGSLEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQNEMVRELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAVLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPGQRKIVMHKIRPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGII

Target ORF information:

RefSeq Version	NM_014676.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014676.3

ORF Insert Sequence:

ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT 
GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACT 
CACTCCAGCC CTGTCCCAGG ATCTATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG 
GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGAGGA 
GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT 
CAGGTGCGTT CCATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA 
GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA 
GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG 
AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGATCGGAGA GTGGGGGACT AGGCGTTAGC 
ATGGTGGAGT ATGTGTTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT 
GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG 
TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT 
GGTTTACCAG TGCAGAATGG GATTGATGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT 
AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG 
GGCTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG 
GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT 
GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC 
TACAATTCTC AACAACAGCT GTTCCAAAGA CCTAATGCGC TTGCTGTCCA GCAGTTGACA 
GCTGCTCAGC AGCAGCAGTA TGCACTGGCA GCTGCTCATC AGCCGCACAT CGGTTTAGCT 
CCCGCTGCGT TTGTCCCCAA TCCATACATC ATCAGCGCTG CTCCCCCAGG GACGGACCCC 
TACACAGCTG GATTGGCTGC AGCAGCGACA CTAGGCCCAG CTGTGGTCCC TCACCAGTAT 
TATGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCTGCCGCT 
GCCGCTGCAG CAACTAATTC AGCTAATCAA CAGACCACCC CACAGGCTCA GCAAGGACAG 
CAGCAGGTTC TCCGTGGAGG AGCCAGCCAA CGTCCTTTGA CCCCAAACCA GAACCAGCAG 
GGACAGCAAA CGGATCCCCT TGTGGCAGCT GCAGCAGTGA ATTCTGCCCT TGCATTTGGA 
CAAGGTCTGG CAGCAGGCAT GCCAGGTTAT CCGGTGTTGG CTCCTGCTGC TTACTATGAC 
CAAACTGGTG CCCTTGTAGT GAATGCAGGC GCGAGAAATG GTCTTGGAGC TCCTGTTCGA 
CTTGTAGCTC CTGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCA 
GCCGCAGCTT CAGCAAATGG AGCAGCTGGT GGTCTTGCTG GAACAACAAA TGGACCATTT 
CGCCCTTTAG GAACACAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAA TAACAACCTG 
GCATCCAGTT CTTTCTACGG CAACAACTCT CTGAACAGCA ATTCACAGAG CAGCTCCCTC 
TTCTCCCAGG GCTCTGCCCA GCCTGCCAAC ACATCCTTGG GATTCGGAAG TAGCAGTTCT 
CTCGGCGCCA CCCTGGGATC CGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC 
ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA 
TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT 
CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT 
CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACGAATGGC 
AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG TGCAAGCAGC 
GCCTCCAGCC TCTTCAGCCC GAGCAGCACT CTTTTCTCTT CCTCTCGTTT GCGATATGGA 
ATGTCTGATG TCATGCCTTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAACAACCGG 
TACCCCAATT TACAACTGCG GGAGATTGCT GGACATATAA TGGAATTTTC CCAAGACCAG 
CATGGGTCCA GATTCATTCA GCTGAAACTG GAGCGTGCCA CACCAGCTGA GCGCCAGCTT 
GTCTTCAATG AAATCCTCCA GGCTGCCTAC CAACTCATGG TGGATGTGTT TGGTAATTAC 
GTCATTCAGA AGTTCTTTGA ATTTGGCAGT CTTGAACAGA AGCTGGCTTT GGCAGAACGG 
ATTCGAGGCC ACGTCCTGTC ATTGGCACTA CAGATGTATG GCTGCCGTGT TATCCAGAAA 
GCTCTTGAGT TTATTCCTTC AGACCAGCAG AATGAGATGG TTCGGGAACT AGATGGCCAT 
GTCTTGAAGT GTGTGAAAGA TCAGAATGGC AATCACGTGG TTCAGAAATG CATTGAATGT 
GTACAGCCCC AGTCTTTGCA ATTTATCATC GATGCGTTTA AGGGACAGGT ATTTGCCTTA 
TCCACACATC CTTATGGCTG CCGAGTGATT CAGAGAATCC TGGAGCACTG TCTCCCTGAC 
CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAGCTTGT ACAGGATCAA 
TATGGAAATT ATGTAATCCA ACATGTACTG GAGCACGGTC GTCCTGAGGA TAAAAGCAAA 
ATTGTAGCAG AAATCCGAGG CAATGTACTT GTATTGAGTC AGCACAAATT TGCAAGCAAT 
GTTGTGGAGA AGTGTGTTAC TCACGCCTCA CGTACGGAGC GCGCTGTGCT CATCGATGAG 
GTGTGCACCA TGAACGACGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT 
GCCAACTACG TGGTCCAGAA GATGATTGAC GTGGCGGAGC CAGGCCAGCG GAAGATCGTC 
ATGCATAAGA TCCGGCCCCA CATCGCAACT CTTCGTAAGT ACACCTATGG CAAGCACATT 
CTGGCCAAGC TGGAGAAGTA CTACATGAAG AACGGTGTTG ACTTAGGGCC CATCTGTGGC 
CCCCCTAATG GTATCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PUM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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