atp2b1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following atp2b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atp2b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa02038 NM_001112935.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
OXa53201 XM_031897582.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53201 XM_031897583.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53201 XM_031897581.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53201 XM_031897580.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53201 XM_031897585.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53201 XM_031897584.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53202 XM_012958492.3
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53203 XM_031897586.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53204 XM_012958495.3
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa30879 NM_001368122.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30878 XM_018091918.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30878 XM_018091919.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30878 XM_012958492.2
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30878 XM_018091920.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30878 XM_018091921.1
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30879 XM_012958493.2
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa30881 XM_012958495.2
Latest version!
Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa02038
    Clone ID Related Accession (Same CDS sequence) NM_001112935.1
    Accession Version NM_001112935.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-28
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC153328.1. ##Evidence-Data-START## Transcript exon combination :: BC153328.1, CX852752.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1696413, SAMEA1696414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    ATGGGAACAT TACTTTGGGG ACAGCTGGTA ACATCAATAC CTACAAGTCG GTTAAAGTTC 
    CTTAAAGAAG CTGGACATGG AACGCAAAAG GATGAAATTC CAGAAGAGGA ACTTGCTGAA
    GATGTGGAGG AGATTGATCA TGCAGAGAGG GAGTTGCGTC GTGGTCAGAT CTTGTGGTTT
    AGGGGCCTAA ACAGAATTCA AACTCAGATG GATGTAGTGA ATGCTTTCCA AAGTGGAAGT
    TCCATTCAGG GGGCTTTAAG GCGGCAACCC TCCATCGCCA GCCAGCATCA TGATGTAACA
    AATATTTCTA CCCCTACACA TGTAGTGTTT TCCTCCTCTA CTGCTTCTAC TACTGTGGGG
    TATTCGAGTG GTGAATGCAT TTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106406.1
    CDS232..618
    Translation

    Target ORF information:

    RefSeq Version NM_001112935.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001112935.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGGGAACAT TACTTTGGGG ACAGCTGGTA ACATCAATAC CTACAAGTCG GTTAAAGTTC 
    CTTAAAGAAG CTGGACATGG AACGCAAAAG GATGAAATTC CAGAAGAGGA ACTTGCTGAA
    GATGTGGAGG AGATTGATCA TGCAGAGAGG GAGTTGCGTC GTGGTCAGAT CTTGTGGTTT
    AGGGGCCTAA ACAGAATTCA AACTCAGATG GATGTAGTGA ATGCTTTCCA AAGTGGAAGT
    TCCATTCAGG GGGCTTTAAG GCGGCAACCC TCCATCGCCA GCCAGCATCA TGATGTAACA
    AATATTTCTA CCCCTACACA TGTAGTGTTT TCCTCCTCTA CTGCTTCTAC TACTGTGGGG
    TATTCGAGTG GTGAATGCAT TTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53201
    Clone ID Related Accession (Same CDS sequence) XM_031897580.1 , XM_031897582.1 , XM_031897584.1 , XM_031897581.1 , XM_031897583.1 , XM_031897585.1
    Accession Version XM_031897580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753440.1
    CDS1267..5076
    Translation

    Target ORF information:

    RefSeq Version XM_031897580.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53201
    Clone ID Related Accession (Same CDS sequence) XM_031897580.1 , XM_031897582.1 , XM_031897584.1 , XM_031897581.1 , XM_031897583.1 , XM_031897585.1
    Accession Version XM_031897582.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753442.1
    CDS555..4364
    Translation

    Target ORF information:

    RefSeq Version XM_031897582.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897582.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53201
    Clone ID Related Accession (Same CDS sequence) XM_031897580.1 , XM_031897582.1 , XM_031897584.1 , XM_031897581.1 , XM_031897583.1 , XM_031897585.1
    Accession Version XM_031897584.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753444.1
    CDS612..4421
    Translation

    Target ORF information:

    RefSeq Version XM_031897584.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53201
    Clone ID Related Accession (Same CDS sequence) XM_031897580.1 , XM_031897582.1 , XM_031897584.1 , XM_031897581.1 , XM_031897583.1 , XM_031897585.1
    Accession Version XM_031897581.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753441.1
    CDS842..4651
    Translation

    Target ORF information:

    RefSeq Version XM_031897581.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897581.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53201
    Clone ID Related Accession (Same CDS sequence) XM_031897580.1 , XM_031897582.1 , XM_031897584.1 , XM_031897581.1 , XM_031897583.1 , XM_031897585.1
    Accession Version XM_031897583.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753443.1
    CDS653..4462
    Translation

    Target ORF information:

    RefSeq Version XM_031897583.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897583.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53201
    Clone ID Related Accession (Same CDS sequence) XM_031897580.1 , XM_031897582.1 , XM_031897584.1 , XM_031897581.1 , XM_031897583.1 , XM_031897585.1
    Accession Version XM_031897585.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753445.1
    CDS350..4159
    Translation

    Target ORF information:

    RefSeq Version XM_031897585.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897585.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCATT GTATGAAGGT
    TTGGAAAAGC CCGAGTCAAG AAGCTCAATC CACAATTTCA TGACGCACCC AGAGTTTAGA
    ATAGAAGATT CCGAGCCTCA TATTCCACTC ATCGATGATA CTGATGCTGA AGATGACGCT
    CCAACAAAAC GACACTCTAC TCCTCCACCC TCCCCCAATA AAAATAACAA TGCTCTTGAC
    AGCGGAATAC ACCTTATTAT AGAACCGAAC AAATCAGCTA CCTCTTCAGC CCCAGGCAGT
    CCACTACATA GTCTGGAGAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53202
    Clone ID Related Accession (Same CDS sequence) XM_012958492.3
    Accession Version XM_012958492.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958492.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813946.1
    CDS1266..5003
    Translation

    Target ORF information:

    RefSeq Version XM_012958492.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958492.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53203
    Clone ID Related Accession (Same CDS sequence) XM_031897586.1
    Accession Version XM_031897586.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATGT AACAAATATT TCTACCCCTA CACATGTAGT GTTTTCCTCC
    TCTACTGCTT CTACTACTGT GGGGTATTCG AGTGGTGAAT GCATTTCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753446.1
    CDS1269..4859
    Translation

    Target ORF information:

    RefSeq Version XM_031897586.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897586.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGGATGGTC CTTTCTCTTG
    TTCTCTCTCT CTCTTGCTGA GCAAGCTGTC ACAATCTCTG ATTCCTTGCA GATGGATGTA
    GTGAATGCTT TCCAAAGTGG AAGTTCCATT CAGGGGGCTT TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATGT AACAAATATT TCTACCCCTA CACATGTAGT GTTTTCCTCC
    TCTACTGCTT CTACTACTGT GGGGTATTCG AGTGGTGAAT GCATTTCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53204
    Clone ID Related Accession (Same CDS sequence) XM_012958495.3
    Accession Version XM_012958495.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958495.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATGTAA CAAATATTTC TACCCCTACA CATGTAGTGT TTTCCTCCTC TACTGCTTCT
    ACTACTGTGG GGTATTCGAG TGGTGAATGC ATTTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813949.1
    CDS1268..4786
    Translation

    Target ORF information:

    RefSeq Version XM_012958495.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958495.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACCTTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATGTAA CAAATATTTC TACCCCTACA CATGTAGTGT TTTCCTCCTC TACTGCTTCT
    ACTACTGTGG GGTATTCGAG TGGTGAATGC ATTTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30879
    Clone ID Related Accession (Same CDS sequence) XM_012958493.2 , NM_001368122.1
    Accession Version NM_001368122.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-11
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03011297.1 and AAMC03011296.1. On Jan 12, 2019 this sequence version replaced XM_012958493.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1696416, SAMEA1696418 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TTCGAGTGGT GAATGCATTT
    CGTAGTTCAT TGTATGAAGG TTTGGAAAAG CCCGAGTCAA GAAGCTCAAT CCACAATTTC
    ATGACGCACC CAGAGTTTAG AATAGAAGAT TCCGAGCCTC ATATTCCACT CATCGATGAT
    ACTGATGCTG AAGATGACGC TCCAACAAAA CGACACTCTA CTCCTCCACC CTCCCCCAAT
    AAAAATAACA ATGCTCTTGA CAGCGGAATA CACCTTATTA TAGAACCGAA CAAATCAGCT
    ACCTCTTCAG CCCCAGGCAG TCCACTACAT AGTCTGGAGA CATCACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001355051.1
    CDS826..4476
    Translation

    Target ORF information:

    RefSeq Version NM_001368122.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001368122.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TTCGAGTGGT GAATGCATTT
    CGTAGTTCAT TGTATGAAGG TTTGGAAAAG CCCGAGTCAA GAAGCTCAAT CCACAATTTC
    ATGACGCACC CAGAGTTTAG AATAGAAGAT TCCGAGCCTC ATATTCCACT CATCGATGAT
    ACTGATGCTG AAGATGACGC TCCAACAAAA CGACACTCTA CTCCTCCACC CTCCCCCAAT
    AAAAATAACA ATGCTCTTGA CAGCGGAATA CACCTTATTA TAGAACCGAA CAAATCAGCT
    ACCTCTTCAG CCCCAGGCAG TCCACTACAT AGTCTGGAGA CATCACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30878
    Clone ID Related Accession (Same CDS sequence) XM_012958492.2 , XM_018091918.1 , XM_018091921.1 , XM_018091919.1 , XM_018091920.1
    Accession Version XM_012958492.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813946.1
    CDS826..4563
    Translation

    Target ORF information:

    RefSeq Version XM_012958492.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958492.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30878
    Clone ID Related Accession (Same CDS sequence) XM_012958492.2 , XM_018091918.1 , XM_018091921.1 , XM_018091919.1 , XM_018091920.1
    Accession Version XM_018091918.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947407.1
    CDS2429..6166
    Translation

    Target ORF information:

    RefSeq Version XM_018091918.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30878
    Clone ID Related Accession (Same CDS sequence) XM_012958492.2 , XM_018091918.1 , XM_018091921.1 , XM_018091919.1 , XM_018091920.1
    Accession Version XM_018091921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947410.1
    CDS498..4235
    Translation

    Target ORF information:

    RefSeq Version XM_018091921.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30878
    Clone ID Related Accession (Same CDS sequence) XM_012958492.2 , XM_018091918.1 , XM_018091921.1 , XM_018091919.1 , XM_018091920.1
    Accession Version XM_018091919.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947408.1
    CDS2331..6068
    Translation

    Target ORF information:

    RefSeq Version XM_018091919.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091919.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30878
    Clone ID Related Accession (Same CDS sequence) XM_012958492.2 , XM_018091918.1 , XM_018091921.1 , XM_018091919.1 , XM_018091920.1
    Accession Version XM_018091920.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947409.1
    CDS2388..6125
    Translation

    Target ORF information:

    RefSeq Version XM_018091920.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091920.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATATTC GAGTGGTGAA TGCATTTCGT AGTTCATTGT ATGAAGGTTT GGAAAAGCCC
    GAGTCAAGAA GCTCAATCCA CAATTTCATG ACGCACCCAG AGTTTAGAAT AGAAGATTCC
    GAGCCTCATA TTCCACTCAT CGATGATACT GATGCTGAAG ATGACGCTCC AACAAAACGA
    CACTCTACTC CTCCACCCTC CCCCAATAAA AATAACAATG CTCTTGACAG CGGAATACAC
    CTTATTATAG AACCGAACAA ATCAGCTACC TCTTCAGCCC CAGGCAGTCC ACTACATAGT
    CTGGAGACAT CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30879
    Clone ID Related Accession (Same CDS sequence) XM_012958493.2 , NM_001368122.1
    Accession Version XM_012958493.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TTCGAGTGGT GAATGCATTT
    CGTAGTTCAT TGTATGAAGG TTTGGAAAAG CCCGAGTCAA GAAGCTCAAT CCACAATTTC
    ATGACGCACC CAGAGTTTAG AATAGAAGAT TCCGAGCCTC ATATTCCACT CATCGATGAT
    ACTGATGCTG AAGATGACGC TCCAACAAAA CGACACTCTA CTCCTCCACC CTCCCCCAAT
    AAAAATAACA ATGCTCTTGA CAGCGGAATA CACCTTATTA TAGAACCGAA CAAATCAGCT
    ACCTCTTCAG CCCCAGGCAG TCCACTACAT AGTCTGGAGA CATCACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813947.1
    CDS826..4476
    Translation

    Target ORF information:

    RefSeq Version XM_012958493.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958493.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TTCGAGTGGT GAATGCATTT
    CGTAGTTCAT TGTATGAAGG TTTGGAAAAG CCCGAGTCAA GAAGCTCAAT CCACAATTTC
    ATGACGCACC CAGAGTTTAG AATAGAAGAT TCCGAGCCTC ATATTCCACT CATCGATGAT
    ACTGATGCTG AAGATGACGC TCCAACAAAA CGACACTCTA CTCCTCCACC CTCCCCCAAT
    AAAAATAACA ATGCTCTTGA CAGCGGAATA CACCTTATTA TAGAACCGAA CAAATCAGCT
    ACCTCTTCAG CCCCAGGCAG TCCACTACAT AGTCTGGAGA CATCACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30881
    Clone ID Related Accession (Same CDS sequence) XM_012958495.2
    Accession Version XM_012958495.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product plasma membrane calcium-transporting ATPase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATGTAA CAAATATTTC TACCCCTACA CATGTAGTGT TTTCCTCCTC TACTGCTTCT
    ACTACTGTGG GGTATTCGAG TGGTGAATGC ATTTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813949.1
    CDS826..4344
    Translation

    Target ORF information:

    RefSeq Version XM_012958495.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ATPase, Ca++ transporting, plasma membrane 1 (atp2b1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958495.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTAATA ATTCAGTTGC ATATAGTGGC GTTAAAAACT CTTTCAATGA AACAAACCAT 
    GGGGGAGAGT TTGGCATTAC AATTGAAGAG CTTCGCACGC TTATGGAATT GAGATCGACG
    GATGCTTTGC AAAAAATACA GGAGCGATAT GGTGATGTGT ATGGAATTTG TACTAGGTTG
    AAAACCTCTC CACATGAAGG TTTAAGCGGG AATCTAGCTG ATCTTGAAAG GAGGCAAGAA
    GCATTTGGGA AGAATATTAT ACCTCCTAAA AAGCCAAAAA CATTTCTTCA GTTAGTATGG
    GAAGCACTAC AAGATGTAAC ATTAATTATT TTAGAAATAG CAGCCATAAT ATCTTTAGGC
    CTTTCTTTTT ACCGTCCTCC TGGTGGAGAT AATGAATTGT GTGGTCAAGC TAGTGGTGCT
    GTAGAGGAAG AAGAAGGGGA AGCAGGCTGG ATTGAAGGAG GTGCTATCCT TGTATCTGTT
    GTATGTGTAG TCTTAGTAAC AGCTTTCAAT GACTGGAGCA AAGAAAAACA ATTTAGGGGC
    TTGCAGAGCC GGATCGAACA AGAGCAGAAA TTTACGGTTG TTCGTGGAGG GCAAGTTATT
    CAAATCCCTG TGGCTGACAT AGTTGTTGGA GATATTGCAC AAATTAAATA CGGTGATCTT
    CTGCCTGCTG ATGGAGTGCT CATTCAAGGA AATGATCTGA AAATTGATGA AAGTTCATTA
    ACTGGAGAAT CTGATCATGT AAAGAAAACG CTTGAAAAAG ATCCTTTGCT GCTTTCAGGA
    ACACATGTCA TGGAGGGGTC TGGGAAAATG GTGGTTACTG CCATTGGTGT GAATTCCCAA
    ACTGGAATAA TTTTTACTTT ACTTGGAGCT GGAGAAAATG AGGAAGAAAA AGAGAAAGAA
    AAAGAAAAAA AGGATAAGAA AAACAAAAAG CAAGATGGCG CCATAGAAAA TCGAAACAAA
    GCAAAAGCTC AAGATGGAGC TGCCATGGAA ATGCAACCAC TAAAGAGTGA AGAGGGAGCT
    GATGGGGATG ATAAAAAGAA AGCAAATTTG CCAAAGAAAG AGAAATCTGT CCTTCAAGGG
    AAACTTACCA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC TTGTGATGTC TGCAATTACA
    GTCATAATCC TTGTTCTCTA CTTCGTAATT AACACATTTT GGGTGCAGAA GCGACCATGG
    CTGGCTGAAT GTACTCCAAT TTATGTTCAA TATTTTGTGA AGTTTTTCAT CATTGGTGTA
    ACTGTTTTAG TAGTAGCTGT TCCAGAAGGT CTTCCACTTG CAGTTACAAT ATCCCTTGCT
    TACTCTGTAA AGAAAATGAT GAAAGATAAC AACTTAGTAA GGCACCTTGA TGCTTGCGAA
    ACAATGGGAA ATGCCACTGC AATTTGTTCA GATAAAACTG GAACATTAAC CATGAACAGG
    ATGACAGTGG TCCAATCGTT CATCAATGAA AAGCATTACC GTAAGGTTCC TGATCCAGAC
    TCTATTTCAG AAAATGTGTT GGATCTCCTC CTAACAGGGA TTTCTGTGAA TTGTGCTTAT
    ACATCGAAAA TATTGCCTCC TGAAAAAGAA GGTGGCTTGC CACGCCATGT TGGTAACAAA
    ACTGAATGTG CCTTGCTGGG ATTTATTTTG GATATGAAAA GGGATTACCA AGATGTAAGA
    AATGAAATCC CTGAGGAAAC CTTATTTAAA GTGTACACTT TTAATTCTGT AAGAAAATCC
    ATGAGCACAG TTTTAAAGAA TGCTGATGGC AGCTACCGGA TGTACAGCAA GGGTGCCTCT
    GAGATTATCC TTAAGAAGTG CTTCAAAACA CTAGGCATAA ATGGTGAGGC CAAAATATTC
    AGACCAAGAG ACAGAGATGA TATGGTTAAA CGTGTTATTG AACCTATGGC TTCAGAAGGG
    CTGCGAACAA TATGTCTAGC GTACCGAGAT TTTCCTGCTG GAGAGCACGA GCCTGACTGG
    GAAAATGAAA ATGATATTCT TACTGGTCTC ACCTGTATTG CTGTTGTAGG AATAGAAGAT
    CCAGTCAGAC CAGAAGTGCC AGATGCAATT AGAAAATGTC AGCGTGCTGG TATAACTGTA
    CGGATGGTTA CAGGGGATAA CATAAATACA GCCCGTGCTA TTGCAACAAA GTGCGGCATT
    CTGCATCCCG GAGAAGATTT CCTTTGTGTA GAAGGCAAAG AATTTAACAG GCGAATACGA
    AACGAAAAAG GAGAGATTGA ACAAGAACGA ATAGACAAAA TTTGGCCTAA ACTTCGTGTT
    CTTGCTAGAT CTTCTCCTAC TGATAAACAT ACACTGGTTA AAGGTATCAT TGACAGTACA
    GTTTGTGAGC AGAGGCAAGT AGTAGCTGTG ACTGGCGATG GGACAAACGA TGGTCCTGCA
    CTGAAGAAAG CAGATGTTGG CTTTGCTATG GGCATTGCTG GAACAGATGT TGCAAAAGAA
    GCGTCTGATA TTATTCTAAC TGATGACAAC TTTAGTAGCA TTGTGAAAGC AGTGATGTGG
    GGAAGGAATG TCTATGACAG TATCTCAAAA TTTCTTCAGT TTCAATTGAC TGTCAATGTG
    GTTGCTGTTA TTGTAGCTTT TACAGGAGCC TGCATTACAC AGGACTCACC CCTTAAAGCT
    GTTCAGATGC TTTGGGTAAA TCTCATTATG GATACATTTG CATCACTTGC TCTGGCGACA
    GAGCCGCCAA CTGAGTCTCT CTTATTAAGG AAACCTTATG GTAGAAATAA ACCCTTAATT
    TCTCGCACAA TGATGAAAAA TATATTGGGC CATGCTGTTT ACCAGCTAGT CGTAGTTTTT
    ACCCTTTTAT TTGTTGGCGA AAAACTTTTT GATATTGACA GTGGAAGAAA TGCACCTCTT
    CATGCTCCTC CTTCACAGCA TTATACAATC GTTTTTAATA CCTTTGTTAT GATGCAACTG
    TTTAATGAAA TAAATGCCAG AAAGATCCAT GGTGAAAGGA ATGTTTTTGA AGGAATATTT
    AACAATATCA TCTTCTGCAG CATTGTATTA GGTACCTTTA TTATACAGAT TGTAATTGTT
    CAGTTTGGTG GAAAGCCATT CAGTTGTACT GAACTCACAG TAGATCAGTG GTTATGGTCT
    ATTTTCCTAG GAATGGGAAC ATTACTTTGG GGACAGCTGG TAACATCAAT ACCTACAAGT
    CGGTTAAAGT TCCTTAAAGA AGCTGGACAT GGAACGCAAA AGGATGAAAT TCCAGAAGAG
    GAACTTGCTG AAGATGTGGA GGAGATTGAT CATGCAGAGA GGGAGTTGCG TCGTGGTCAG
    ATCTTGTGGT TTAGGGGCCT AAACAGAATT CAAACTCAGA TGGATGTAGT GAATGCTTTC
    CAAAGTGGAA GTTCCATTCA GGGGGCTTTA AGGCGGCAAC CCTCCATCGC CAGCCAGCAT
    CATGATGTAA CAAATATTTC TACCCCTACA CATGTAGTGT TTTCCTCCTC TACTGCTTCT
    ACTACTGTGG GGTATTCGAG TGGTGAATGC ATTTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.