spag1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following spag1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spag1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa22935 NM_001170493.1
Latest version!
Xenopus tropicalis sperm associated antigen 1 (spag1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OXa47266 NM_001374761.1
Latest version!
Xenopus tropicalis sperm associated antigen 1 (spag1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa59462 XM_031903153.1
Latest version!
Xenopus tropicalis sperm associated antigen 1 (spag1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa22935
    Clone ID Related Accession (Same CDS sequence) NM_001170493.1
    Accession Version NM_001170493.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-28
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm-associated antigen 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC159317.1. ##Evidence-Data-START## Transcript exon combination :: BC159317.1, EL662834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1025683, SAMEA1025684 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGGAATG CACACAATAA GCCCTTTCCC AAGAAAAACT GCCCCAGGAG CGAGGAATGT 
    GGGGCCCCCA GTCACACACA GAATGGAGTG AAGAAAGACT GCGGCAATGG ACAGCAGCCA
    AGGAAACCAT GCGAAGGGGA CAGCCTTAAG CAGAACAGAG TGCCAATCCC TGGCACTGAG
    AACACAGCAA AGAGTGAAAT TAAGGAAGTG AAGCCTTCCT CAGAGCCCCT TCCTACAGCG
    GCACGGCTGA AGTCAGAAGG CAACCAGCTG TTTAAGAATG GCCAGTTTGC TGAGGCTGCA
    CTCAAGTATT CAGAAGCCAT AGAAAATGTT AAGAACACAC GATCAGAGAA TGCGGAGGAA
    CTGGCCATCT TGCATTCTAA CAGAGCTGCT TGCCACTTGA AGGATGGCAA TAGCAGAGAG
    TGCATTGAAG ATTGCAACAG GGCATTGGAG CTGCAGCCAT TCTCAGTGAA GCCGCTTTTA
    CGCCGGGCGA TGGCTAATGA GTCCCTGGAG AGATACAGAC CAGCATATGT GGATTATAAA
    ACTGTCTTGC AGATTGACAG TTCCATGCAA GTTGCACATG ACAGTATCAA CAGGATTACA
    AGAACGTTAA TAGAACAAGA TGGGCCCGAC TGGAGAGAAA AACTGCCCCC AATTCCCACT
    GTACCGATCT CCGTTCACCT ACAGCAACAT GGAGGTGTCC CTGCTTCCAG TAGCAGCCAG
    CCCAAGATTC TGGTGGAACA TGGTACATAT ACACAGCACT CAGTGGATAT GTACAGTGGT
    GCAGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163964.1
    CDS6..794
    Translation

    Target ORF information:

    RefSeq Version NM_001170493.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm associated antigen 1 (spag1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170493.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGGAATG CACACAATAA GCCCTTTCCC AAGAAAAACT GCCCCAGGAG CGAGGAATGT 
    GGGGCCCCCA GTCACACACA GAATGGAGTG AAGAAAGACT GCGGCAATGG ACAGCAGCCA
    AGGAAACCAT GCGAAGGGGA CAGCCTTAAG CAGAACAGAG TGCCAATCCC TGGCACTGAG
    AACACAGCAA AGAGTGAAAT TAAGGAAGTG AAGCCTTCCT CAGAGCCCCT TCCTACAGCG
    GCACGGCTGA AGTCAGAAGG CAACCAGCTG TTTAAGAATG GCCAGTTTGC TGAGGCTGCA
    CTCAAGTATT CAGAAGCCAT AGAAAATGTT AAGAACACAC GATCAGAGAA TGCGGAGGAA
    CTGGCCATCT TGCATTCTAA CAGAGCTGCT TGCCACTTGA AGGATGGCAA TAGCAGAGAG
    TGCATTGAAG ATTGCAACAG GGCATTGGAG CTGCAGCCAT TCTCAGTGAA GCCGCTTTTA
    CGCCGGGCGA TGGCTAATGA GTCCCTGGAG AGATACAGAC CAGCATATGT GGATTATAAA
    ACTGTCTTGC AGATTGACAG TTCCATGCAA GTTGCACATG ACAGTATCAA CAGGATTACA
    AGAACGTTAA TAGAACAAGA TGGGCCCGAC TGGAGAGAAA AACTGCCCCC AATTCCCACT
    GTACCGATCT CCGTTCACCT ACAGCAACAT GGAGGTGTCC CTGCTTCCAG TAGCAGCCAG
    CCCAAGATTC TGGTGGAACA TGGTACATAT ACACAGCACT CAGTGGATAT GTACAGTGGT
    GCAGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47266
    Clone ID Related Accession (Same CDS sequence) NM_001374761.1
    Accession Version NM_001374761.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm-associated antigen 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03024800.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTGCAC AGGACATTTC CTCTGTAATG AACTATGGAA TGACCAAAAC CCATCAAATC 
    CCTGTTGACC ACCTTGATTA CAGTTTCATA GAGAACTGTT CAGACATCAA ACATCTGGAA
    AAGATTTTGT GGGTTCTCAG GTCTGGTGAA GAAGGACATT ACCCAGAACT AATAGTGTTT
    TGTGAGGAAC GGATTGGGAA TTTAGCTCCT CACAGTCGTG CTTTGCGGAA AGACAAACCC
    TCTGCCACTG CCTCTGACTT CTCATCAGAA GACTGGACAA ATATTGCCAA TGATTTAAAG
    GAGTGGCTGG TGGATATGAA AATGAAAGAG CAAAACGAAT CTGTTATTCC ACCTGACACA
    GAAAATCTTC CATCTATACG GAGTTACACT AGTCTCCCCG CTGACAATAA GCAGAGAGGA
    AAAGAGAGGA CAGGATTGAG CAAAAAAGCC CCACGGGACT ACAGAGACTG GGATAAATTT
    GATGTGGAGA AAGAGTTGTC AAAAATTGAA GATAACCCTG AGACAAAGCA ACAATCAAAG
    ACAACAATAA ATCCTAAAAC ATCAGCTATT AAGAAGACGT TAGACACAGC AGGTTTGAGC
    CCGACACAAA GAAGATTTAT TGCAGAAAAT GAGAAAGAAA AGGGAAATGA AGCTTTCAGA
    TCTGGGGACT ACCAGGAAGC AATAGTATAT TATAGCAGGA GCATTTCTGT GTTTCCCTCA
    GCAGCAGCAT ATAACAACAG AGCACAAGCA GAGATCAAGC TGAGTAACTG GCGGAAGGCA
    CTGAATGACT GTGAGAGAGT GCTGGAGTTA GATCCTGGAA ACACCAAAGC ATATTTACGG
    AGGGCCACAG CCCATAAGAA CCTGGCCAAT TACAAGGCAG CAACAACTGA TCTAAATAGA
    GTCTTAAGTC AGGAACCAGA TAACCCAGTT GCTAAGAAAG AGTTTGCTGA AGTCGAGGAG
    CTGCTGCAGA AGGCAGAGGA AGCGAGTCGG TGCAAAGGAA GGAAAATTGT TATTGAGGAC
    ATAGAAGGGT CAGATGAAGA CGAAGCCGGT GTGTCAGTGG GAGGAGAGCC AGCGGGGGAG
    CACAGTGACA TGGGGAATGC ACACAATAAG CCCTTTCCCA AGAAAAACTG CCCCAGGAGC
    GAGGAATGTG GGGCCCCCAG TCACACACAG AATGGAGTGA AGAAAGACTG CGGCAATGGA
    CAGCAGCCAA GGAAACCATG CGAAGGGGAC AGCCTTAAGC AGAACAGAGT GCCAATCCCT
    GGCACTGAGA ACACAGCAAA GAGTGAAATT AAGGAAGTGA AGCCTTCCTC AGAGCCCCTT
    CCTACAGCGG CACGGCTGAA GTCAGAAGGC AACCAGCTGT TTAAGAATGG CCAGTTTGCT
    GAGGCTGCAC TCAAGTATTC AGAAGCCATA GAAAATGTTA AGAACACACG ATCAGAGAAT
    GCGGAGGAAC TGGCCATCTT GCATTCTAAC AGAGCTGCTT GCCACTTGAA GGATGGCAAT
    AGCAGAGAGT GCATTGAAGA TTGCAACAGG GCATTGGAGC TGCAGCCATT CTCAGTGAAG
    CCGCTTTTAC GCCGGGCGAT GGCTAATGAG TCCCTGGAGA GATACAGACC AGCATATGTG
    GATTATAAAA CTGTCTTGCA GATTGACAGT TCCATGCAAG TTGCACATGA CAGTATCAAC
    AGGATTACAA GAACGTTAAT AGAACAAGAT GGGCCCGACT GGAGAGAAAA ACTGCCCCCA
    ATTCCCACTG TACCGATCTC CGTTCACCTA CAGCAACATG GAGGTGTCCC TGCTTCCAGT
    AGCAGCCAGC CCAAGATTCT GGTGGAACAT GGTAAATCGC CCGAGGAGAT GTTGCAGGCT
    CTGAAAACCG AAGGCAATGA GTGTGTGAAG AAAGGACAAT ACAGAGAGGC TGAGAAGAAG
    TATAGTGAAT GCTTGAAGAT AAATGCAGAA GAATGTACCT TGTACACAAA TAGGGCCCTG
    TGTTACTTGA AACTGAGTGA GTACGAGGCG GCCAGGAAGG ACTGTGAATG CGCTCTGCAG
    AGGGACGCTT TAAATATCAA AGCCTTGTAC AGACGAGCAC AGGCCTACAG AGGATTAGAG
    GATTATCAGG CGTGTGCTAA TGATCTCCGA AAAGTCATCT CTGTGGATCC TAGTATTACA
    GAAGCAAAGA AACTGCTGGA TGAAATAGCT CCCTTTCTTA CAGCAGCTGA TGGTCTTGTC
    AAGGAGAAGG AAAAGCAGAG AAAGAAGATC CTAATAGAGG AGGTGAATGA GGCCAATGTG
    GATGGGGAAG TGTCTGATAA TAAACACTGC AATGGGCAAG AAAGGAGTGC AACACTGGCA
    GCCACAACAC GGTCTCCCAT TACCAAACCT GCCAACGCCT ATGAGTTTGG CCAGCAGTTA
    AATGATATAA AAGTGAGGAA AGACGAGACT GGCTGTGCGG AACTCTTGTC TGTCACCGAG
    CCAAAGGACT TGCCTGCGCT TATGAGCAAC AAGTTAGAGG AGGACACTCT GCTGCTAATC
    GTGCGCTCCA TGAAACACAG TCTGCTTCCC ACGGCCCCCG CTCTGGTATT CCAGCATCTC
    TCCCACCTGA GCAAAGCCGA GAGGTTTAAG ATAGTGATCA TGCTCTCCAG TAAGAGTGAA
    AAGGATGAAA TTCGGGATCT CTTTGATTCC CTCGCTCAGA ACCAGAATGG GGATATGGAG
    CGGGAGGCCA TACTGAGTCT GGCAAAGGAA TACGACATTT CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361690.1
    CDS58..2805
    Translation

    Target ORF information:

    RefSeq Version NM_001374761.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm associated antigen 1 (spag1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374761.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTGCAC AGGACATTTC CTCTGTAATG AACTATGGAA TGACCAAAAC CCATCAAATC 
    CCTGTTGACC ACCTTGATTA CAGTTTCATA GAGAACTGTT CAGACATCAA ACATCTGGAA
    AAGATTTTGT GGGTTCTCAG GTCTGGTGAA GAAGGACATT ACCCAGAACT AATAGTGTTT
    TGTGAGGAAC GGATTGGGAA TTTAGCTCCT CACAGTCGTG CTTTGCGGAA AGACAAACCC
    TCTGCCACTG CCTCTGACTT CTCATCAGAA GACTGGACAA ATATTGCCAA TGATTTAAAG
    GAGTGGCTGG TGGATATGAA AATGAAAGAG CAAAACGAAT CTGTTATTCC ACCTGACACA
    GAAAATCTTC CATCTATACG GAGTTACACT AGTCTCCCCG CTGACAATAA GCAGAGAGGA
    AAAGAGAGGA CAGGATTGAG CAAAAAAGCC CCACGGGACT ACAGAGACTG GGATAAATTT
    GATGTGGAGA AAGAGTTGTC AAAAATTGAA GATAACCCTG AGACAAAGCA ACAATCAAAG
    ACAACAATAA ATCCTAAAAC ATCAGCTATT AAGAAGACGT TAGACACAGC AGGTTTGAGC
    CCGACACAAA GAAGATTTAT TGCAGAAAAT GAGAAAGAAA AGGGAAATGA AGCTTTCAGA
    TCTGGGGACT ACCAGGAAGC AATAGTATAT TATAGCAGGA GCATTTCTGT GTTTCCCTCA
    GCAGCAGCAT ATAACAACAG AGCACAAGCA GAGATCAAGC TGAGTAACTG GCGGAAGGCA
    CTGAATGACT GTGAGAGAGT GCTGGAGTTA GATCCTGGAA ACACCAAAGC ATATTTACGG
    AGGGCCACAG CCCATAAGAA CCTGGCCAAT TACAAGGCAG CAACAACTGA TCTAAATAGA
    GTCTTAAGTC AGGAACCAGA TAACCCAGTT GCTAAGAAAG AGTTTGCTGA AGTCGAGGAG
    CTGCTGCAGA AGGCAGAGGA AGCGAGTCGG TGCAAAGGAA GGAAAATTGT TATTGAGGAC
    ATAGAAGGGT CAGATGAAGA CGAAGCCGGT GTGTCAGTGG GAGGAGAGCC AGCGGGGGAG
    CACAGTGACA TGGGGAATGC ACACAATAAG CCCTTTCCCA AGAAAAACTG CCCCAGGAGC
    GAGGAATGTG GGGCCCCCAG TCACACACAG AATGGAGTGA AGAAAGACTG CGGCAATGGA
    CAGCAGCCAA GGAAACCATG CGAAGGGGAC AGCCTTAAGC AGAACAGAGT GCCAATCCCT
    GGCACTGAGA ACACAGCAAA GAGTGAAATT AAGGAAGTGA AGCCTTCCTC AGAGCCCCTT
    CCTACAGCGG CACGGCTGAA GTCAGAAGGC AACCAGCTGT TTAAGAATGG CCAGTTTGCT
    GAGGCTGCAC TCAAGTATTC AGAAGCCATA GAAAATGTTA AGAACACACG ATCAGAGAAT
    GCGGAGGAAC TGGCCATCTT GCATTCTAAC AGAGCTGCTT GCCACTTGAA GGATGGCAAT
    AGCAGAGAGT GCATTGAAGA TTGCAACAGG GCATTGGAGC TGCAGCCATT CTCAGTGAAG
    CCGCTTTTAC GCCGGGCGAT GGCTAATGAG TCCCTGGAGA GATACAGACC AGCATATGTG
    GATTATAAAA CTGTCTTGCA GATTGACAGT TCCATGCAAG TTGCACATGA CAGTATCAAC
    AGGATTACAA GAACGTTAAT AGAACAAGAT GGGCCCGACT GGAGAGAAAA ACTGCCCCCA
    ATTCCCACTG TACCGATCTC CGTTCACCTA CAGCAACATG GAGGTGTCCC TGCTTCCAGT
    AGCAGCCAGC CCAAGATTCT GGTGGAACAT GGTAAATCGC CCGAGGAGAT GTTGCAGGCT
    CTGAAAACCG AAGGCAATGA GTGTGTGAAG AAAGGACAAT ACAGAGAGGC TGAGAAGAAG
    TATAGTGAAT GCTTGAAGAT AAATGCAGAA GAATGTACCT TGTACACAAA TAGGGCCCTG
    TGTTACTTGA AACTGAGTGA GTACGAGGCG GCCAGGAAGG ACTGTGAATG CGCTCTGCAG
    AGGGACGCTT TAAATATCAA AGCCTTGTAC AGACGAGCAC AGGCCTACAG AGGATTAGAG
    GATTATCAGG CGTGTGCTAA TGATCTCCGA AAAGTCATCT CTGTGGATCC TAGTATTACA
    GAAGCAAAGA AACTGCTGGA TGAAATAGCT CCCTTTCTTA CAGCAGCTGA TGGTCTTGTC
    AAGGAGAAGG AAAAGCAGAG AAAGAAGATC CTAATAGAGG AGGTGAATGA GGCCAATGTG
    GATGGGGAAG TGTCTGATAA TAAACACTGC AATGGGCAAG AAAGGAGTGC AACACTGGCA
    GCCACAACAC GGTCTCCCAT TACCAAACCT GCCAACGCCT ATGAGTTTGG CCAGCAGTTA
    AATGATATAA AAGTGAGGAA AGACGAGACT GGCTGTGCGG AACTCTTGTC TGTCACCGAG
    CCAAAGGACT TGCCTGCGCT TATGAGCAAC AAGTTAGAGG AGGACACTCT GCTGCTAATC
    GTGCGCTCCA TGAAACACAG TCTGCTTCCC ACGGCCCCCG CTCTGGTATT CCAGCATCTC
    TCCCACCTGA GCAAAGCCGA GAGGTTTAAG ATAGTGATCA TGCTCTCCAG TAAGAGTGAA
    AAGGATGAAA TTCGGGATCT CTTTGATTCC CTCGCTCAGA ACCAGAATGG GGATATGGAG
    CGGGAGGCCA TACTGAGTCT GGCAAAGGAA TACGACATTT CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59462
    Clone ID Related Accession (Same CDS sequence) XM_031903153.1
    Accession Version XM_031903153.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm-associated antigen 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTGCAC AGGACATTTC CTCTGTAATG AACTATGGAA TGACCAAAAC CCATCAAATC 
    CCTGTTGACC ACCTTGATTA CAGTTTCATA GAGAACTGTT CAGACATCAA ACATCTGGAA
    AAGATTTTGT GGGTTCTCAG GTCTGGTGAA GAAGGACATT ACCCAGAACT AATAGTGTTT
    TGTGAGGAAC AGATTGGGAA TTTAGCTCCT CACAGTCGTG CTTTGCGGAA AGACAAACCC
    CCTGCCACTG CCTCTGACTT CTCATCAGAA GACTGGACAA ATATTGCCAA TGATTTAAAG
    GAGTGGCTGG TGGATATGAA AATGAAAGAG CAAAACGAAT CTGTTATTCC ACCTGACACA
    GAAAATCTTC CACCTATACG GAGTTACACT AGTCTCCCCG CTGACAATAA GAGAGGAAAA
    GAGAGGACAG GATTGAGCAA AAAAGCCCCA CGGGACTACA GAGACTGGGA TAAATTTGAT
    GTGGAGAAAG AGTTGTCAAA AATTGAAGAT AACCCTGAGA CAAAGCAACA ATCAAAGACA
    ACAATAAATC CTAAAACATC AGCTATTAAG AAGACGTTAG ACACAGCAGG TTTGAGCCCG
    ACACAAAGAA GATTTATTGC AGAAAATGAG AAAGAAAAGG GAAATGAAGC TTTCAGATCT
    GGGGACTACC AGGAAGCAAT AGTATATTAT AGCAGGAGCA TTTCTGTGTT TCCCTCAGCA
    GCAGCATATA ACAACAGAGC ACAAGCAGAG ATCAAGCTGA GTAACTGGCG GAAGGCACTG
    AATGACTGTG AGAGAGTGCT GGAGTTAGAT CCTGGAAACA CCAAAGCATA TTTACGGAGG
    GCCACAGCCC ATAAGAACCT GGCCAATTAC AAGGCAGCAA CAACTGATCT AAATAGAGTC
    TTAAGTCAGG AACCAGATAA CCCAGTTGCT AAGAAAGAGT TTGCTGAAGT CGAGGAGCTG
    CTGCAGAAGG CAGAGGAAGC GAGTCGGTGC AAAGGAAGGA AAATTGTTAT TGAGGACATA
    GAAGGGTCAG ATGAAGACGA AGCCGGTGTG TCAGTGGGAG GAGAGCCAGC GGGGGAGCAC
    AGTGACATGG GGAATGCACA CAATAAGCCC TTTCCCAAGA AAAACTGCCC CAGGAGCGAG
    GAATGTGGGG CCCCCAGTCA CACACAGAAT GGAGTGAAGA AAGACTGCGG CAATGGACAG
    CAGCCAAGGA AACCATGCGA AGGGGACAGC CTTAAGCAGA ACAGAGTGCC AATCCCTGGC
    ACTGAGAACA CAGCAAAGAG TGAAATTAAG GAAGTGAAGC CTTCCTCAGA GCCCCTTCCT
    ACAGCGGCAC GGCTGAAGTC AGAAGGCAAC CAGCTGTTTA AGAATGGCCA GTTTGCTGAG
    GCTGCACTCA AGTATTCAGA AGCCATAGAA AATGTTAAGA ACACACGATC AGAGAATGCG
    GAGGAACTGG CCATCTTGCA TTCTAACAGA GCTGCTTGCC ACTTGAAGGA TGGCAATAGC
    AGAGAGTGCA TTGAAGATTG CAACAGGGCA TTGGAGCTGC AGCCATTCTC AGTGAAGCCG
    CTTTTACGCC GGGCGATGGC TAATGAGTCC CTGGAGAGAT ACAGACCAGC ATATGTGGAT
    TATAAAACTG TCTTGCAGAT TGACAGTTCC ATGCAAGTTG CACATGACAG TATCAACAGG
    ATTACAAGAA CGTTAATAGA ACAAGATGGG CCCGACTGGA GAGAAAAACT GCCCCCAATT
    CCCACTGTAC CGATCTCCGT TCACCTACAG CAACATGGAG GTGTCCCTGC TTCCAGTAGC
    AGCCAGCCCA AGATTCTGGT GGAACATGGT AAATCGCCCG AGGAGATGTT GCAGGCTCTG
    AAAACCGAAG GCAATGAGTG TGTGAAGAAA GGACAATACA GAGAGGCTGA GAAGAAGTAT
    AGTGAATGCT TGAAGATAAA TGCAGAAGAA TGTACCTTGT ACACAAATAG GGCCCTGTGT
    TACTTGAAAC TGAGTGAGTA CGAGGCGGCC AGGAAGGACT GTGAATGCGC TCTGCAGAGG
    GACGCTTTAA ATATCAAAGC CTTGTACAGA CGAGCACAGG CCTACAGAGG ATTAGAGGAT
    TATCAGGCGT GTGCTAATGA TCTCCGAAAA GTCATCTCTG TGGATCCTAG TATTACAGAA
    GCAAAGAAAC TGCTGGATGA AATAGCTCCC TTTCTTACAG CAGCTGATGG TCTTGTCAAG
    GAGAAGGAAA AGCAGAGAAA GAAGATCCTA ATAGAGGAGG TGAATGAGGC CAATGTGGAT
    GGGGAAGTGT CTGATAATAA ACACTGCAAT GGGCAAGAAA GGAGTGCAAC AATGGCAGCC
    ACAACACGGT CTCCCATTAC CAGACCTGCC AACGCCTATG AGTTTGGCCA GCAGTTAAAT
    GATATAAAAG TGAGGAAAGA CGAGACTGGC TGTGCGGAAC TCTTGTCTGT CACCGAGCCA
    AAGGACTTGC CTGCGCTTAT GAGCAACAAG TTAGAGGAGG ACACTCTGCT GCTAATCGTG
    CGCTCCATGA AACACAGTCT GCTTCCCACG GCCCCCGCTC TGGTATTCCA GCATCTCTCC
    CACCTGAGCA AAGCCGAGAG GTTTAAGATA GTGATCATGC TCTCCAGTAA GAGTGAAAAG
    GATGAAATTC GGGATCTCTT TGATTCCCTC GCTCAGAACC AGAATGGGGA TATGGAGCGG
    GAGGCCATAC TGAGTCTGGC AAAGGAATAC GACATTTCCC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759013.1
    CDS91..2835
    Translation

    Target ORF information:

    RefSeq Version XM_031903153.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm associated antigen 1 (spag1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTGCAC AGGACATTTC CTCTGTAATG AACTATGGAA TGACCAAAAC CCATCAAATC 
    CCTGTTGACC ACCTTGATTA CAGTTTCATA GAGAACTGTT CAGACATCAA ACATCTGGAA
    AAGATTTTGT GGGTTCTCAG GTCTGGTGAA GAAGGACATT ACCCAGAACT AATAGTGTTT
    TGTGAGGAAC AGATTGGGAA TTTAGCTCCT CACAGTCGTG CTTTGCGGAA AGACAAACCC
    CCTGCCACTG CCTCTGACTT CTCATCAGAA GACTGGACAA ATATTGCCAA TGATTTAAAG
    GAGTGGCTGG TGGATATGAA AATGAAAGAG CAAAACGAAT CTGTTATTCC ACCTGACACA
    GAAAATCTTC CACCTATACG GAGTTACACT AGTCTCCCCG CTGACAATAA GAGAGGAAAA
    GAGAGGACAG GATTGAGCAA AAAAGCCCCA CGGGACTACA GAGACTGGGA TAAATTTGAT
    GTGGAGAAAG AGTTGTCAAA AATTGAAGAT AACCCTGAGA CAAAGCAACA ATCAAAGACA
    ACAATAAATC CTAAAACATC AGCTATTAAG AAGACGTTAG ACACAGCAGG TTTGAGCCCG
    ACACAAAGAA GATTTATTGC AGAAAATGAG AAAGAAAAGG GAAATGAAGC TTTCAGATCT
    GGGGACTACC AGGAAGCAAT AGTATATTAT AGCAGGAGCA TTTCTGTGTT TCCCTCAGCA
    GCAGCATATA ACAACAGAGC ACAAGCAGAG ATCAAGCTGA GTAACTGGCG GAAGGCACTG
    AATGACTGTG AGAGAGTGCT GGAGTTAGAT CCTGGAAACA CCAAAGCATA TTTACGGAGG
    GCCACAGCCC ATAAGAACCT GGCCAATTAC AAGGCAGCAA CAACTGATCT AAATAGAGTC
    TTAAGTCAGG AACCAGATAA CCCAGTTGCT AAGAAAGAGT TTGCTGAAGT CGAGGAGCTG
    CTGCAGAAGG CAGAGGAAGC GAGTCGGTGC AAAGGAAGGA AAATTGTTAT TGAGGACATA
    GAAGGGTCAG ATGAAGACGA AGCCGGTGTG TCAGTGGGAG GAGAGCCAGC GGGGGAGCAC
    AGTGACATGG GGAATGCACA CAATAAGCCC TTTCCCAAGA AAAACTGCCC CAGGAGCGAG
    GAATGTGGGG CCCCCAGTCA CACACAGAAT GGAGTGAAGA AAGACTGCGG CAATGGACAG
    CAGCCAAGGA AACCATGCGA AGGGGACAGC CTTAAGCAGA ACAGAGTGCC AATCCCTGGC
    ACTGAGAACA CAGCAAAGAG TGAAATTAAG GAAGTGAAGC CTTCCTCAGA GCCCCTTCCT
    ACAGCGGCAC GGCTGAAGTC AGAAGGCAAC CAGCTGTTTA AGAATGGCCA GTTTGCTGAG
    GCTGCACTCA AGTATTCAGA AGCCATAGAA AATGTTAAGA ACACACGATC AGAGAATGCG
    GAGGAACTGG CCATCTTGCA TTCTAACAGA GCTGCTTGCC ACTTGAAGGA TGGCAATAGC
    AGAGAGTGCA TTGAAGATTG CAACAGGGCA TTGGAGCTGC AGCCATTCTC AGTGAAGCCG
    CTTTTACGCC GGGCGATGGC TAATGAGTCC CTGGAGAGAT ACAGACCAGC ATATGTGGAT
    TATAAAACTG TCTTGCAGAT TGACAGTTCC ATGCAAGTTG CACATGACAG TATCAACAGG
    ATTACAAGAA CGTTAATAGA ACAAGATGGG CCCGACTGGA GAGAAAAACT GCCCCCAATT
    CCCACTGTAC CGATCTCCGT TCACCTACAG CAACATGGAG GTGTCCCTGC TTCCAGTAGC
    AGCCAGCCCA AGATTCTGGT GGAACATGGT AAATCGCCCG AGGAGATGTT GCAGGCTCTG
    AAAACCGAAG GCAATGAGTG TGTGAAGAAA GGACAATACA GAGAGGCTGA GAAGAAGTAT
    AGTGAATGCT TGAAGATAAA TGCAGAAGAA TGTACCTTGT ACACAAATAG GGCCCTGTGT
    TACTTGAAAC TGAGTGAGTA CGAGGCGGCC AGGAAGGACT GTGAATGCGC TCTGCAGAGG
    GACGCTTTAA ATATCAAAGC CTTGTACAGA CGAGCACAGG CCTACAGAGG ATTAGAGGAT
    TATCAGGCGT GTGCTAATGA TCTCCGAAAA GTCATCTCTG TGGATCCTAG TATTACAGAA
    GCAAAGAAAC TGCTGGATGA AATAGCTCCC TTTCTTACAG CAGCTGATGG TCTTGTCAAG
    GAGAAGGAAA AGCAGAGAAA GAAGATCCTA ATAGAGGAGG TGAATGAGGC CAATGTGGAT
    GGGGAAGTGT CTGATAATAA ACACTGCAAT GGGCAAGAAA GGAGTGCAAC AATGGCAGCC
    ACAACACGGT CTCCCATTAC CAGACCTGCC AACGCCTATG AGTTTGGCCA GCAGTTAAAT
    GATATAAAAG TGAGGAAAGA CGAGACTGGC TGTGCGGAAC TCTTGTCTGT CACCGAGCCA
    AAGGACTTGC CTGCGCTTAT GAGCAACAAG TTAGAGGAGG ACACTCTGCT GCTAATCGTG
    CGCTCCATGA AACACAGTCT GCTTCCCACG GCCCCCGCTC TGGTATTCCA GCATCTCTCC
    CACCTGAGCA AAGCCGAGAG GTTTAAGATA GTGATCATGC TCTCCAGTAA GAGTGAAAAG
    GATGAAATTC GGGATCTCTT TGATTCCCTC GCTCAGAACC AGAATGGGGA TATGGAGCGG
    GAGGCCATAC TGAGTCTGGC AAAGGAATAC GACATTTCCC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.