LOC100258559 cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100258559
Entrez Gene ID	100258559
Full Name	polygalacturonase 1 beta-like protein 1
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002266305.3	XP_002266341.1	polygalacturonase 1 beta-like protein 1

Pathways from BioSystems
Gene Ontology

The following LOC100258559 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100258559 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa00672	XM_002266305.3 Latest version!	Vitis vinifera polygalacturonase 1 beta-like protein 1 (LOC100258559), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100258559 ( XM_002266305.3 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_002266341.1 Vitis vinifera polygalacturonase 1 beta-like protein 1 (LOC100258559), mRNA.

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CloneID	OVa00672
Clone ID Related Accession (Same CDS sequence)	XM_002266305.3
Accession Version	XM_002266305.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-21
Organism	Vitis vinifera(wine grape)
Product	polygalacturonase 1 beta-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012007.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 22, 2016 this sequence version replaced XM_002266305.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAGAC AACATAAGCT TTTGAGCCAT CTCTTTCTCA TTTTCTTATT CTCAGCTTCG 
TTTCCCAATG TTGCTTTGGG CGGAGCCGGT GAGGAATCGG GCAAACCGGT GGCTGGAGAA 
AACCCATTCA CACCCAGAGC GTCTTTGCTT CGGTATTGGA ATAAGCAGAT CGGCAGTGGC 
TTGCCAAAGT CCACCTTTCT TCTCTCCAAG GCATCCCCAT TGAGCGCGGT TGAGTCGGCC 
ACCTTTACTA AACTTGCCGC TCAAAATGCG CTTTCCGACA ATCTGCCGGC GTTCTGTAAG 
TCTGCGAACC TGCTCTGTTT TCCCGATTTG GGGCAGAGCT TGGAGAAGCA TGACGCTAGT 
TCCAACTTCG CCGTGTATTC GAACAAGAAT TTCACCAATT ACGGAACGGA TGGACTCGGG 
GGAACGGACG CCTTCACAAA ATACTCGGAG GGGGTCAATC TTCCTGTCGA CGCATTCAAG 
CGCTATAGCC GTGACTCCGT CGGCAACAAT GATAAATTCG ATAATTACGG CCGCGAGGGC 
AACGTCGTGG ATCAGAGCTT CAGCGGCTAC GGTGCCGGGG CTACCGGCGG CTCTGGAGAG 
TTCAAGAAGT ACAACGAAGA GGTTAATGTT CCCAACCTTC GATTCAACTC CTACACAGAC 
GATGGCAATG GCCGTCAACA ATCATTCACG AGTTACACAA ATGAGACCAA TTCTGGAGAT 
GAGTCCTTCA GTAGCTACGG CAAGAACGGC AACGGATCTC CGAACGAGTT CACGAGTTAC 
GGCAGCAGCT CCAACGTCAT CGGCTCCACG TTCACCGGCT ACGGTGAAAC CGGCAATGCC 
GCCAACGATA CGTTCAAGAG CTACGGCTTT GACGGCAACG TGCCAGAGAA CAACTTCAAG 
AAATACGGTG ATGGTGGTAA TGCGGGGACC GATACTTTCA TAAGTTACAG AGATCAATCC 
AATGTAGGAG ACGACTCCTT CAAATCCTAC GCCAAGAATT CCAATTCCGC AGAGGTGGAT 
TTCGTCAACT ATGGGAAATC GTTCAACGAA GGTACAGACA CTTTCGCTGG GTACGGCAAG 
GGAGCGACGA ACCACAAAAT TAATTTCAAG ATCTACGGCG TCAACAACAC ATTCACAGAC 
TACGCTAAAA AGGGCATCTC CTTTTCAAGA TACACCAACA AAAGCTCTGA AACAATGACT 
TCCATGGCGG TCAGTGGCAG TTCAGTAAAT AGGTGGGTTG AGCCGGGTAA ATTCTTCCGC 
GAGTCGATGC TGAAGAAAGG GACTGTGATG CCAATGCCGG ACATTAGGGA TAAAATGCCC 
AAAAGGTCGT TTTTGCCCCG CACTATTTCC TCCAAATTTC CGTTTTCAAC CTCCAAACTT 
GAGGAGATGA AGAAAATATT CCATGCCGCG GATAACTCAA GCATGGAGCA CATGTTCACA 
GAGGCATTAG ATGACTGCGA GAGAGCACCC AGCAAAGGCG AGACCAAGCG CTGCGTCCCC 
TCCATCGAGG ACATGATCGA CTTCGCCACC ACCGTCCTCG GCCGCAACGT TGTGGTTCGC 
ACCACCCAAT CCGTCGAAGG GTCAAAACAA AACTTAATGA TCGGGTCGGT CAAAGGGATC 
AACGGTGGAC AAGTCACCAA ATCGGTGTCC TGCCACCAGA GCCTGTTCCC TTATCTACTC 
TATTACTGCC ACTCCGTTCC CAAGGTTCGG GTCTATGAAG CGGACCTCCT GGACCCGAAA 
ACCAAAGCCA ACGTTAACCA TGGTGTTGCC ATCTGTCACT TGGACACCTC CGATTGGAGC 
GCGGGTCATG GTGCATTCGT GGCATTGGGT TCGGGCCCTG GCCGGATTGA AGTTTGTCAC 
TGGATTTTCG AGAATGATAT GACCTGGACC ATTGCAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002266341.1
CDS	76..1977
Translation	MHRQHKLLSHLFLIFLFSASFPNVALGGAGEESGKPVAGENPFTPRASLLRYWNKQIGSGLPKSTFLLSKASPLSAVESATFTKLAAQNALSDNLPAFCKSANLLCFPDLGQSLEKHDASSNFAVYSNKNFTNYGTDGLGGTDAFTKYSEGVNLPVDAFKRYSRDSVGNNDKFDNYGREGNVVDQSFSGYGAGATGGSGEFKKYNEEVNVPNLRFNSYTDDGNGRQQSFTSYTNETNSGDESFSSYGKNGNGSPNEFTSYGSSSNVIGSTFTGYGETGNAANDTFKSYGFDGNVPENNFKKYGDGGNAGTDTFISYRDQSNVGDDSFKSYAKNSNSAEVDFVNYGKSFNEGTDTFAGYGKGATNHKINFKIYGVNNTFTDYAKKGISFSRYTNKSSETMTSMAVSGSSVNRWVEPGKFFRESMLKKGTVMPMPDIRDKMPKRSFLPRTISSKFPFSTSKLEEMKKIFHAADNSSMEHMFTEALDDCERAPSKGETKRCVPSIEDMIDFATTVLGRNVVVRTTQSVEGSKQNLMIGSVKGINGGQVTKSVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPKTKANVNHGVAICHLDTSDWSAGHGAFVALGSGPGRIEVCHWIFENDMTWTIAD

Target ORF information:

RefSeq Version	XM_002266305.3
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera polygalacturonase 1 beta-like protein 1 (LOC100258559), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002266305.3

ORF Insert Sequence:

ATGCATAGAC AACATAAGCT TTTGAGCCAT CTCTTTCTCA TTTTCTTATT CTCAGCTTCG 
TTTCCCAATG TTGCTTTGGG CGGAGCCGGT GAGGAATCGG GCAAACCGGT GGCTGGAGAA 
AACCCATTCA CACCCAGAGC GTCTTTGCTT CGGTATTGGA ATAAGCAGAT CGGCAGTGGC 
TTGCCAAAGT CCACCTTTCT TCTCTCCAAG GCATCCCCAT TGAGCGCGGT TGAGTCGGCC 
ACCTTTACTA AACTTGCCGC TCAAAATGCG CTTTCCGACA ATCTGCCGGC GTTCTGTAAG 
TCTGCGAACC TGCTCTGTTT TCCCGATTTG GGGCAGAGCT TGGAGAAGCA TGACGCTAGT 
TCCAACTTCG CCGTGTATTC GAACAAGAAT TTCACCAATT ACGGAACGGA TGGACTCGGG 
GGAACGGACG CCTTCACAAA ATACTCGGAG GGGGTCAATC TTCCTGTCGA CGCATTCAAG 
CGCTATAGCC GTGACTCCGT CGGCAACAAT GATAAATTCG ATAATTACGG CCGCGAGGGC 
AACGTCGTGG ATCAGAGCTT CAGCGGCTAC GGTGCCGGGG CTACCGGCGG CTCTGGAGAG 
TTCAAGAAGT ACAACGAAGA GGTTAATGTT CCCAACCTTC GATTCAACTC CTACACAGAC 
GATGGCAATG GCCGTCAACA ATCATTCACG AGTTACACAA ATGAGACCAA TTCTGGAGAT 
GAGTCCTTCA GTAGCTACGG CAAGAACGGC AACGGATCTC CGAACGAGTT CACGAGTTAC 
GGCAGCAGCT CCAACGTCAT CGGCTCCACG TTCACCGGCT ACGGTGAAAC CGGCAATGCC 
GCCAACGATA CGTTCAAGAG CTACGGCTTT GACGGCAACG TGCCAGAGAA CAACTTCAAG 
AAATACGGTG ATGGTGGTAA TGCGGGGACC GATACTTTCA TAAGTTACAG AGATCAATCC 
AATGTAGGAG ACGACTCCTT CAAATCCTAC GCCAAGAATT CCAATTCCGC AGAGGTGGAT 
TTCGTCAACT ATGGGAAATC GTTCAACGAA GGTACAGACA CTTTCGCTGG GTACGGCAAG 
GGAGCGACGA ACCACAAAAT TAATTTCAAG ATCTACGGCG TCAACAACAC ATTCACAGAC 
TACGCTAAAA AGGGCATCTC CTTTTCAAGA TACACCAACA AAAGCTCTGA AACAATGACT 
TCCATGGCGG TCAGTGGCAG TTCAGTAAAT AGGTGGGTTG AGCCGGGTAA ATTCTTCCGC 
GAGTCGATGC TGAAGAAAGG GACTGTGATG CCAATGCCGG ACATTAGGGA TAAAATGCCC 
AAAAGGTCGT TTTTGCCCCG CACTATTTCC TCCAAATTTC CGTTTTCAAC CTCCAAACTT 
GAGGAGATGA AGAAAATATT CCATGCCGCG GATAACTCAA GCATGGAGCA CATGTTCACA 
GAGGCATTAG ATGACTGCGA GAGAGCACCC AGCAAAGGCG AGACCAAGCG CTGCGTCCCC 
TCCATCGAGG ACATGATCGA CTTCGCCACC ACCGTCCTCG GCCGCAACGT TGTGGTTCGC 
ACCACCCAAT CCGTCGAAGG GTCAAAACAA AACTTAATGA TCGGGTCGGT CAAAGGGATC 
AACGGTGGAC AAGTCACCAA ATCGGTGTCC TGCCACCAGA GCCTGTTCCC TTATCTACTC 
TATTACTGCC ACTCCGTTCC CAAGGTTCGG GTCTATGAAG CGGACCTCCT GGACCCGAAA 
ACCAAAGCCA ACGTTAACCA TGGTGTTGCC ATCTGTCACT TGGACACCTC CGATTGGAGC 
GCGGGTCATG GTGCATTCGT GGCATTGGGT TCGGGCCCTG GCCGGATTGA AGTTTGTCAC 
TGGATTTTCG AGAATGATAT GACCTGGACC ATTGCAGACT GA