PRKCB cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKCB
Entrez Gene ID	100522792
Full Name	protein kinase C beta
Synonyms	PKC,PRKCB1,PRKCB_tv2
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 3 Map Location: 3\|3

mRNA and Protein(s)

mRNA	Protein	Name
XM_021086459.1	XP_020942118.1	protein kinase C beta type isoform X1
XM_021086460.1	XP_020942119.1	protein kinase C beta type isoform X2
XM_013995603.1	XP_013851057.1	protein kinase C beta type

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Uenishi H, Eguchi-Ogawa T, Shinkai H, Okumura N, Suzuki K, Toki D, Hamasima N, Awata T
Nucleic acids research35(Database issue)D650-3(2007 Jan)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

first study to show the expression and cellular localization of PKC beta I in the retina of pigs with development, and these results suggest that PKC beta I, in accordance with PKC alpha, plays important roles in signal transduction pathways in the pig retina with development
Title: Immunohistochemical localization of protein kinase C (PKC) beta I in the pig retina during postnatal development.

Increases in epicardial perivascular adipose tissue leptin exacerbate coronary endothelial dysfunction in MetS via a PKC-beta-dependent pathway.
Title: Epicardial perivascular adipose-derived leptin exacerbates coronary endothelial dysfunction in metabolic syndrome via a protein kinase C-beta pathway.

The PKCalpha, PKCbeta1, and PKCbeta2 mRNA levels tended to be lower in ischemia-reperfused than in sham-operated eyes in both the retinal arteries and the neuroretina.
Title: Protein kinase C in porcine retinal arteries and neuroretina following retinal ischemia-reperfusion.

ET-1-mediated myocardial contractile dysfunction during cardioplegia involves activation of protein kinase C beta II.
Title: Protein kinase C isoform activation and endothelin-1 mediated defects in myocyte contractility after cardioplegic arrest and reperfusion.

The following PRKCB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKCB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe245699	XM_021086459.1 Latest version!	Sus scrofa protein kinase C beta (PRKCB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OSe245700	XM_021086460.1 Latest version!	Sus scrofa protein kinase C beta (PRKCB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKCB ( XM_021086459.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020942118.1 Sus scrofa protein kinase C beta (PRKCB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe245699
Clone ID Related Accession (Same CDS sequence)	XM_021086459.1
Accession Version	XM_021086459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	protein kinase C beta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGTGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTCCAGTG CCAAGTCTGC TGTTTTGTTG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC AGGCGCTGAC AAGGGCCCAG CCTCTGATGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCC 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAGTGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT GCCCAGCCTG TGTGGCACAG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGAGAGG GAGGTTCTCA TCGTGGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC TTGTCAGACC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGCGA GAGCAAGCAG AAGACCAAAA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAGACATT TAGATTCCAG CTGAAAGAGT CAGACAAAGA CAGAAGACTG 
TCTGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCT 
TTTGGGATTT CCGAACTGCA GAAAGCCGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTACCTGTG CCACCAGAAG GAGGGGAGGG CAATGAGGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC GGTCAGGGGA CCAAGACTCC CGAAGAGAAG 
ACGACCAACA CCATATCCAA GTTCGATAAC AACGGGAACA GGGACCGGAT GAAACTGACT 
GATTTTAACT TCTTGATGGT GTTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCCGAG 
CGGAAGGGCA CAGATGAGCT GTACGCGGTG AAGATCCTGA AAAAGGATGT GGTGATTCAA 
GACGATGATG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTATGTGA ATGGTGGCGA CCTCATGTAC CACATTCAGC AAGTGGGCCG CTTCAAAGAG 
CCTCACGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGTATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC GACCAAGACC 
TTCTGTGGCA CCCCAGACTA CATCGCCCCT GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGCG AGGATGAGGA TGAACTTTTC CAGTCCATCA TGGAACACAA CGTGGCATAT 
CCAAAGTCCA TGTCCAAGGA AGCCGTGGCC ATCTGCAAAG GGTTGATGAC CAAACACCCG 
GGCAAACGAC TGGGTTGCGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCGTTTTTC 
CGGTATATCG ACTGGGAGAA ACTCGAACGC AAAGAGATTC AGCCCCCATA TAAGCCGAAA 
GCCTGTGGGC GAAATGCTGA AAACTTCGAC CGATTTTTCA CCCGCCATCC ACCAGTCCTA 
ACACCTCCTG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC 
TTTGTTAACT CTGAATTTTT AAAACCTGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020942118.1
CDS	557..2578
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIEREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGGEGNEELRQKFERAKIGQGTKTPEEKTTNTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS

Target ORF information:

RefSeq Version	XM_021086459.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa protein kinase C beta (PRKCB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021086459.1

ORF Insert Sequence:

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGTGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTCCAGTG CCAAGTCTGC TGTTTTGTTG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC AGGCGCTGAC AAGGGCCCAG CCTCTGATGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCC 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAGTGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT GCCCAGCCTG TGTGGCACAG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGAGAGG GAGGTTCTCA TCGTGGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC TTGTCAGACC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGCGA GAGCAAGCAG AAGACCAAAA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAGACATT TAGATTCCAG CTGAAAGAGT CAGACAAAGA CAGAAGACTG 
TCTGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCT 
TTTGGGATTT CCGAACTGCA GAAAGCCGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTACCTGTG CCACCAGAAG GAGGGGAGGG CAATGAGGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC GGTCAGGGGA CCAAGACTCC CGAAGAGAAG 
ACGACCAACA CCATATCCAA GTTCGATAAC AACGGGAACA GGGACCGGAT GAAACTGACT 
GATTTTAACT TCTTGATGGT GTTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCCGAG 
CGGAAGGGCA CAGATGAGCT GTACGCGGTG AAGATCCTGA AAAAGGATGT GGTGATTCAA 
GACGATGATG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTATGTGA ATGGTGGCGA CCTCATGTAC CACATTCAGC AAGTGGGCCG CTTCAAAGAG 
CCTCACGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGTATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC GACCAAGACC 
TTCTGTGGCA CCCCAGACTA CATCGCCCCT GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGCG AGGATGAGGA TGAACTTTTC CAGTCCATCA TGGAACACAA CGTGGCATAT 
CCAAAGTCCA TGTCCAAGGA AGCCGTGGCC ATCTGCAAAG GGTTGATGAC CAAACACCCG 
GGCAAACGAC TGGGTTGCGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCGTTTTTC 
CGGTATATCG ACTGGGAGAA ACTCGAACGC AAAGAGATTC AGCCCCCATA TAAGCCGAAA 
GCCTGTGGGC GAAATGCTGA AAACTTCGAC CGATTTTTCA CCCGCCATCC ACCAGTCCTA 
ACACCTCCTG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC 
TTTGTTAACT CTGAATTTTT AAAACCTGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCB ( XM_021086460.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020942119.1 Sus scrofa protein kinase C beta (PRKCB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe245700
Clone ID Related Accession (Same CDS sequence)	XM_021086460.1
Accession Version	XM_021086460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	protein kinase C beta type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGTGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTCCAGTG CCAAGTCTGC TGTTTTGTTG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC AGGCGCTGAC AAGGGCCCAG CCTCTGATGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCC 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAGTGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT GCCCAGCCTG TGTGGCACAG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGAGAGG GAGGTTCTCA TCGTGGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC TTGTCAGACC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGCGA GAGCAAGCAG AAGACCAAAA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAGACATT TAGATTCCAG CTGAAAGAGT CAGACAAAGA CAGAAGACTG 
TCTGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCT 
TTTGGGATTT CCGAACTGCA GAAAGCCGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTACCTGTG CCACCAGAAG GAGGGGAGGG CAATGAGGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC GGTCAGGGGA CCAAGACTCC CGAAGAGAAG 
ACGACCAACA CCATATCCAA GTTCGATAAC AACGGGAACA GGGACCGGAT GAAACTGACT 
GATTTTAACT TCTTGATGGT GTTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCCGAG 
CGGAAGGGCA CAGATGAGCT GTACGCGGTG AAGATCCTGA AAAAGGATGT GGTGATTCAA 
GACGATGATG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTATGTGA ATGGTGGCGA CCTCATGTAC CACATTCAGC AAGTGGGCCG CTTCAAAGAG 
CCTCACGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGTATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC GACCAAGACC 
TTCTGTGGCA CCCCAGACTA CATCGCCCCT GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGCG AGGATGAGGA TGAACTTTTC CAGTCCATCA TGGAACACAA CGTGGCATAT 
CCAAAGTCCA TGTCCAAGGA AGCCGTGGCC ATCTGCAAAG GGTTGATGAC CAAACACCCG 
GGCAAACGAC TGGGTTGCGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCGTTTTTC 
CGGTATATCG ACTGGGAGAA ACTCGAACGC AAAGAGATTC AGCCCCCATA TAAGCCGAAA 
GCCAGAGACA AGCGAGACAC CTCCAATTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA 
CTCACGCCTA CTGATAAACT CTTCATCATG AACTTGGACC AAAACGAATT TGCTGGCTTC 
TCCTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020942119.1
CDS	557..2572
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIEREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGGEGNEELRQKFERAKIGQGTKTPEEKTTNTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV

Target ORF information:

RefSeq Version	XM_021086460.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa protein kinase C beta (PRKCB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021086460.1

ORF Insert Sequence:

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGTGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTCCAGTG CCAAGTCTGC TGTTTTGTTG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC AGGCGCTGAC AAGGGCCCAG CCTCTGATGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCC 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAGTGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT GCCCAGCCTG TGTGGCACAG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGAGAGG GAGGTTCTCA TCGTGGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC TTGTCAGACC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGCGA GAGCAAGCAG AAGACCAAAA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAGACATT TAGATTCCAG CTGAAAGAGT CAGACAAAGA CAGAAGACTG 
TCTGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCT 
TTTGGGATTT CCGAACTGCA GAAAGCCGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTACCTGTG CCACCAGAAG GAGGGGAGGG CAATGAGGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC GGTCAGGGGA CCAAGACTCC CGAAGAGAAG 
ACGACCAACA CCATATCCAA GTTCGATAAC AACGGGAACA GGGACCGGAT GAAACTGACT 
GATTTTAACT TCTTGATGGT GTTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCCGAG 
CGGAAGGGCA CAGATGAGCT GTACGCGGTG AAGATCCTGA AAAAGGATGT GGTGATTCAA 
GACGATGATG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTATGTGA ATGGTGGCGA CCTCATGTAC CACATTCAGC AAGTGGGCCG CTTCAAAGAG 
CCTCACGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGTATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC GACCAAGACC 
TTCTGTGGCA CCCCAGACTA CATCGCCCCT GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGCG AGGATGAGGA TGAACTTTTC CAGTCCATCA TGGAACACAA CGTGGCATAT 
CCAAAGTCCA TGTCCAAGGA AGCCGTGGCC ATCTGCAAAG GGTTGATGAC CAAACACCCG 
GGCAAACGAC TGGGTTGCGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCGTTTTTC 
CGGTATATCG ACTGGGAGAA ACTCGAACGC AAAGAGATTC AGCCCCCATA TAAGCCGAAA 
GCCAGAGACA AGCGAGACAC CTCCAATTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA 
CTCACGCCTA CTGATAAACT CTTCATCATG AACTTGGACC AAAACGAATT TGCTGGCTTC 
TCCTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Nucleic acids research35(Database issue)D650-3(2007 Jan)
Uenishi H,Eguchi-Ogawa T,Shinkai H,Okumura N,Suzuki K,Toki D,Hamasima N,Awata T

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T

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PRKCB cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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FAX

オンラインフォーム