rffl cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene
Clones
gRNAs

Summary

Gene Symbol	rffl
Entrez Gene ID	108922482
Full Name	ring finger and FYVE like domain containing E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Scleropages formosus(Asian bonytongue)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018732655.1	XP_018588171.1	E3 ubiquitin-protein ligase rififylin isoform X2
XM_018732654.1	XP_018588170.1	E3 ubiquitin-protein ligase rififylin isoform X1

Pathways from BioSystems
Gene Ontology

The following rffl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rffl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc40047	XM_018732654.1 Latest version!	Scleropages formosus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OSc40048	XM_018732655.1 Latest version!	Scleropages formosus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rffl ( XM_018732654.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018588170.1 Scleropages formosus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSc40047
Clone ID Related Accession (Same CDS sequence)	XM_018732654.1
Accession Version	XM_018732654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	E3 ubiquitin-protein ligase rififylin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041815.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTG CCTGCTGCAA CTGGTTTTGT CTGGAGAGCA CAACAGTGGA TGACGCTAGC 
AGTGCTCGGC CACAAGCCTA CACCAACTCT GGGTACACCA GCTACCCTTC ACCTGTAGCC 
CCCGATCACG TTTGCAAGGC TTGCCGGCTC CTTTTTGATT CCCAGAACAG GAAGCATGTT 
TGCATGGACT GCAAGAAGAG CTTTTGCAGC CGTTGCTCAG TGCAGCTGGG GCAACGCCCA 
CGTCTCTGCC TCACCTGCCA GCTGTTCTAT GGTGCACTCC AGAACCGCAT TGCCCTTATG 
AAGTTCAAGG TAAAGGATTT GCGAGACTAC CTGCACCTCC ATGGCATCTC CACCCAGATG 
TGCCGGGAAA AGGAGGAGCT TGTGGAACTG GTGTTGGGTC AGCAGACTCC CAGTAGCACC 
AACAGCACTG AACCCACCTT GGTGTCAGCC ACAGCTCCAC CATCCTCAGC AGCTCCAATG 
CCACCTGAAC CACCCCCGCC CCCCACTGAG TCATCTGATC TCGAGCCATT AACTCAGGAA 
GACAACCAGA GTCCGTCTGA GCCTGTGGCC TCCGGTCAAG AGGGAACACC TGACTCTCAG 
ACCGAAACAG AGGTCCAGGG GCCACAGGAG CTGCAGTCCT CAGACTCTGA GGAGACCATC 
ACCCTTGGTC GCAGAGCTTC ACTGTCAGAC CTGAGTCAGG TGGAAGACAT TGAGGCCCTC 
AGCGTTCGGC AGCTAAAAGA GATCCTGGCC CGCAATTTTG TGGACTACAA GGGCTGCTGT 
GAGAAGTGGG AGCTCATGGA GAGGGTCACT CGCCTCTTCA AGGAGCAGAA GGACCTCCAA 
GACCTGGTTT TAAGTGCTGA AGATGCTGCA GAGTCCGGTG GTGCTCCTTC CCGGGAGGAA 
AACTTGTGCA AGATCTGCAT GGACTCACCC ATCGACTGTG TGCTGTTGGA ATGTGGTCAC 
ATGGTCACCT GTACCAAGTG CGGCAAGCGC ATGAGCGAGT GCCCCATCTG CAGGCAGTAT 
GTGGTGCGTG CTGTACATGT CTTCAGGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018588170.1
CDS	95..1147
Translation	MWSACCNWFCLESTTVDDASSARPQAYTNSGYTSYPSPVAPDHVCKACRLLFDSQNRKHVCMDCKKSFCSRCSVQLGQRPRLCLTCQLFYGALQNRIALMKFKVKDLRDYLHLHGISTQMCREKEELVELVLGQQTPSSTNSTEPTLVSATAPPSSAAPMPPEPPPPPTESSDLEPLTQEDNQSPSEPVASGQEGTPDSQTETEVQGPQELQSSDSEETITLGRRASLSDLSQVEDIEALSVRQLKEILARNFVDYKGCCEKWELMERVTRLFKEQKDLQDLVLSAEDAAESGGAPSREENLCKICMDSPIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFRS

Target ORF information:

RefSeq Version	XM_018732654.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018732654.1

ORF Insert Sequence:

ATGTGGTCTG CCTGCTGCAA CTGGTTTTGT CTGGAGAGCA CAACAGTGGA TGACGCTAGC 
AGTGCTCGGC CACAAGCCTA CACCAACTCT GGGTACACCA GCTACCCTTC ACCTGTAGCC 
CCCGATCACG TTTGCAAGGC TTGCCGGCTC CTTTTTGATT CCCAGAACAG GAAGCATGTT 
TGCATGGACT GCAAGAAGAG CTTTTGCAGC CGTTGCTCAG TGCAGCTGGG GCAACGCCCA 
CGTCTCTGCC TCACCTGCCA GCTGTTCTAT GGTGCACTCC AGAACCGCAT TGCCCTTATG 
AAGTTCAAGG TAAAGGATTT GCGAGACTAC CTGCACCTCC ATGGCATCTC CACCCAGATG 
TGCCGGGAAA AGGAGGAGCT TGTGGAACTG GTGTTGGGTC AGCAGACTCC CAGTAGCACC 
AACAGCACTG AACCCACCTT GGTGTCAGCC ACAGCTCCAC CATCCTCAGC AGCTCCAATG 
CCACCTGAAC CACCCCCGCC CCCCACTGAG TCATCTGATC TCGAGCCATT AACTCAGGAA 
GACAACCAGA GTCCGTCTGA GCCTGTGGCC TCCGGTCAAG AGGGAACACC TGACTCTCAG 
ACCGAAACAG AGGTCCAGGG GCCACAGGAG CTGCAGTCCT CAGACTCTGA GGAGACCATC 
ACCCTTGGTC GCAGAGCTTC ACTGTCAGAC CTGAGTCAGG TGGAAGACAT TGAGGCCCTC 
AGCGTTCGGC AGCTAAAAGA GATCCTGGCC CGCAATTTTG TGGACTACAA GGGCTGCTGT 
GAGAAGTGGG AGCTCATGGA GAGGGTCACT CGCCTCTTCA AGGAGCAGAA GGACCTCCAA 
GACCTGGTTT TAAGTGCTGA AGATGCTGCA GAGTCCGGTG GTGCTCCTTC CCGGGAGGAA 
AACTTGTGCA AGATCTGCAT GGACTCACCC ATCGACTGTG TGCTGTTGGA ATGTGGTCAC 
ATGGTCACCT GTACCAAGTG CGGCAAGCGC ATGAGCGAGT GCCCCATCTG CAGGCAGTAT 
GTGGTGCGTG CTGTACATGT CTTCAGGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rffl ( XM_018732655.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018588171.1 Scleropages formosus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSc40048
Clone ID Related Accession (Same CDS sequence)	XM_018732655.1
Accession Version	XM_018732655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	E3 ubiquitin-protein ligase rififylin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041815.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTG CCTGCTGCAA CTGGTTTTGT CTGGAGAGCA CAACAGTGGA TGACGCTAGC 
AGTGCTCGGC CACAAGCCTA CACCAACTCT GGGTACACCA GCTACCCTTC ACCTGTAGCC 
CCCGATCACG TTTGCAAGGC TTGCCGGCTC CTTTTTGATT CCCAGAACAG GAAGCATGTT 
TGCATGGACT GCAAGAAGAG CTTTTGCAGC CGTTGCTCAG TGCAGCTGGG GCAACGCCCA 
CGTCTCTGCC TCACCTGCCA GCTGTTCTAT GGTGCACTCC AGAACCGCAT TGCCCTTATG 
AAGTTCAAGG TAAAGGATTT GCGAGACTAC CTGCACCTCC ATGGCATCTC CACCCAGATG 
TGCCGGGAAA AGGAGGAGCT TGTGGAACTG GTGTTGGGTC AGCAGACTCC CAGTAGCACC 
AACAGCACTG AACCCACCTT GGTGTCAGCC ACAGCTCCAC CATCCTCAGC AGCTCCAATG 
CCACCTGAAC CACCCCCGCC CCCCACTGAG TCATCTGATC TCGAGCCATT AACTCAGGAA 
GACAACCAGT CCTCAGACTC TGAGGAGACC ATCACCCTTG GTCGCAGAGC TTCACTGTCA 
GACCTGAGTC AGGTGGAAGA CATTGAGGCC CTCAGCGTTC GGCAGCTAAA AGAGATCCTG 
GCCCGCAATT TTGTGGACTA CAAGGGCTGC TGTGAGAAGT GGGAGCTCAT GGAGAGGGTC 
ACTCGCCTCT TCAAGGAGCA GAAGGACCTC CAAGACCTGG TTTTAAGTGC TGAAGATGCT 
GCAGAGTCCG GTGGTGCTCC TTCCCGGGAG GAAAACTTGT GCAAGATCTG CATGGACTCA 
CCCATCGACT GTGTGCTGTT GGAATGTGGT CACATGGTCA CCTGTACCAA GTGCGGCAAG 
CGCATGAGCG AGTGCCCCAT CTGCAGGCAG TATGTGGTGC GTGCTGTACA TGTCTTCAGG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018588171.1
CDS	95..1060
Translation	MWSACCNWFCLESTTVDDASSARPQAYTNSGYTSYPSPVAPDHVCKACRLLFDSQNRKHVCMDCKKSFCSRCSVQLGQRPRLCLTCQLFYGALQNRIALMKFKVKDLRDYLHLHGISTQMCREKEELVELVLGQQTPSSTNSTEPTLVSATAPPSSAAPMPPEPPPPPTESSDLEPLTQEDNQSSDSEETITLGRRASLSDLSQVEDIEALSVRQLKEILARNFVDYKGCCEKWELMERVTRLFKEQKDLQDLVLSAEDAAESGGAPSREENLCKICMDSPIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFRS

Target ORF information:

RefSeq Version	XM_018732655.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018732655.1

ORF Insert Sequence:

ATGTGGTCTG CCTGCTGCAA CTGGTTTTGT CTGGAGAGCA CAACAGTGGA TGACGCTAGC 
AGTGCTCGGC CACAAGCCTA CACCAACTCT GGGTACACCA GCTACCCTTC ACCTGTAGCC 
CCCGATCACG TTTGCAAGGC TTGCCGGCTC CTTTTTGATT CCCAGAACAG GAAGCATGTT 
TGCATGGACT GCAAGAAGAG CTTTTGCAGC CGTTGCTCAG TGCAGCTGGG GCAACGCCCA 
CGTCTCTGCC TCACCTGCCA GCTGTTCTAT GGTGCACTCC AGAACCGCAT TGCCCTTATG 
AAGTTCAAGG TAAAGGATTT GCGAGACTAC CTGCACCTCC ATGGCATCTC CACCCAGATG 
TGCCGGGAAA AGGAGGAGCT TGTGGAACTG GTGTTGGGTC AGCAGACTCC CAGTAGCACC 
AACAGCACTG AACCCACCTT GGTGTCAGCC ACAGCTCCAC CATCCTCAGC AGCTCCAATG 
CCACCTGAAC CACCCCCGCC CCCCACTGAG TCATCTGATC TCGAGCCATT AACTCAGGAA 
GACAACCAGT CCTCAGACTC TGAGGAGACC ATCACCCTTG GTCGCAGAGC TTCACTGTCA 
GACCTGAGTC AGGTGGAAGA CATTGAGGCC CTCAGCGTTC GGCAGCTAAA AGAGATCCTG 
GCCCGCAATT TTGTGGACTA CAAGGGCTGC TGTGAGAAGT GGGAGCTCAT GGAGAGGGTC 
ACTCGCCTCT TCAAGGAGCA GAAGGACCTC CAAGACCTGG TTTTAAGTGC TGAAGATGCT 
GCAGAGTCCG GTGGTGCTCC TTCCCGGGAG GAAAACTTGT GCAAGATCTG CATGGACTCA 
CCCATCGACT GTGTGCTGTT GGAATGTGGT CACATGGTCA CCTGTACCAA GTGCGGCAAG 
CGCATGAGCG AGTGCCCCAT CTGCAGGCAG TATGTGGTGC GTGCTGTACA TGTCTTCAGG 
TCCTAA