tlh2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	tlh2
Entrez Gene ID	5802845
Full Name	RecQ type DNA helicase Tlh1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001713106.2	XP_001713158.1	RecQ type DNA helicase Tlh1
NM_001355852.1	NP_001343051.1	RecQ type DNA helicase Tlh1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	tlh1	NP_595040.1
Schizosaccharomyces pombe (fission yeast)	tlh2	XP_001713158.1
Schizosaccharomyces pombe (fission yeast)	SPAC212.06c	XP_004001758.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tlh2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tlh2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101621	NM_001355852.1 Latest version!	Schizosaccharomyces pombe RecQ type DNA helicase Tlh1 (tlh2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSj04973	XM_001713106.2 Latest version!	Schizosaccharomyces pombe 972h- RecQ type DNA helicase Tlh1 (tlh2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tlh2 ( NM_001355852.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001343051.1 Schizosaccharomyces pombe RecQ type DNA helicase Tlh1 (tlh2), partial mRNA.

Price & Availability↑

CloneID	OSc101621
Clone ID Related Accession (Same CDS sequence)	NM_001355852.1
Accession Version	NM_001355852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	RecQ type DNA helicase Tlh1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). COMPLETENESS: incomplete on the 5' end.

ATGGTCGTCG CTTCAGAAAT TGCTAAAGTC GCTTCAAAAA CTGCTAGAGA TATCGCCGGA 
TGCTTTACTT GTCAATGTGG AACTCAATTT GATAATGTAG AAAGGATCGT TCAACATTTC 
AAGGAATGTC GATACAGAGA CGAAACTTGT AAAGACGATG ATATCGTCGT CTATGAACCC 
AGCTCTTTCG TTCAGGACGA GAAAAAGGAT AAGCCAATCA TCGTTGAGGC TGCCAGTGAG 
GCTACCAGTG AGGAGGCTTG CAATTCTTCC AAGGAGCGTC AACTACCCGC TCTCTCTGCT 
CTTTCTGCTC TCTCTACTCT CACTACGAGT GCAAATGACG ACTTGTGGAC TGCACGGCTA 
ATTTGGCAAA GTACAAATGA CACCAAATTG GACAACAGCC CATCATCAAA TTATACTGAC 
TTGAATCACA AGTTGGCTAA TTATGGACTC TCCATTTTGT CTATTCATGC TTTGATGTGC 
GTAGAATGTG AATGCTTGTT AAACGTGATT CACACTGCGC AACATATGCA AATTGTGCAT 
AAACTAGAGT TAAACGAAGA TCTTTTGTGG TTTCAAGAAC TAAGGACTTT AAAGTTAAAA 
AGTCCAACAA ATGTTTTACA AACTCATTCT TCTCAAACCC ATGTATACCC ATACATCAGA 
GGACTACCAG TACTTTTAAA TGGATATGAG TGTGTGCCGT GCACAAAAAA CGGAACAGGA 
TTCGTACATG CAATCATGGA TACCTTTCGC CATCATGTTC GACGTACTCA TGGAAAAGTT 
ATAAAATTGG AAAATTGTAT ACGTCGAACA GCTTTGCAAA CGGTTAAGAA CAAGTATGCT 
CAACGATGTC AGTTCTTCAA AGTCGATTAT GTACCTTTGA ATGGAGGAGA AGAAGAAGAA 
GAGGAGGAGG GGGAGGAGAA AGAAGATGCA CAAAACATCA AGGAAAGAAT GGTGGACTTT 
TGCTTTTCAA AATTTATGGA GAAGAATCAA CAGCGGCGGG AGCAGCAAGA CAAAGGTGAA 
AATAAAAAAC GACAAGATGA TGTTGACCAA GCAACAGACA ACAACACTAA CACTATTCTT 
GAAGATGATG AAAAAGATAA CGATGAAGAG GAAGAGGAAG AGATTGTCAA TGCACGAGAG 
AAAAATCTTT TAAATCAACA ATTCAATTGG ACGGCAATTG TGAAAAAACT TGGAGAAAAT 
TGGGATCAGC TGGTTCGATT CGAATATACG AATGGAATTG TAACTTTGGA TACCATTGTA 
AACCAGTTGA TTCGTTATTA CTATCGAGGA TTCCGTCATC TAAGTGGAAT GACCATGGGC 
ATGAGACGAA TGTTTACTCA AGGAGGAAGC TATAGTGCAC AAGAGCGAGG ACTTTGTCGC 
TTAGAGCAAA AGGATACAGT TGTGAGATAT GCTCAAAGTG CAGCACTATA CCTAATATTT 
CTGTTACGTC GTCCATCAGC GGACTCGGGG ATAAGAAGAC ACTTGGAAGC AATGTGTGGA 
GCAACAGTGG AGAGAAAAGA AGGTGGCAGT AACAGCAGCA GTAACATCAG CAACGTCGCC 
AACTTTGATA GTGCTGAAGA CGACAATGAC AATGACAATG ACAATGACAG AGACAGTAAC 
AATAACAATA ACAATAACAA CACCAATACT GACGATGATG ATAAGCTGGC ATACTTGGAA 
CTGCACGAGG CATTAAAATT GGCTTTTCTG CAACAATACG ATTTTTCTAA AAATGTTCAA 
GACTTGGAAA TCATGGAGTT TCTTGCATGC ATGTCTTTGC ATAAAGATGG TACTTCAAAA 
TATGCCTATG AAATCTCTGC CTGCTTTGCA CCCCTAATAT ACACGTGTCG TCTTGTAGCA 
GCATGTGAGT TACAACGATT GATCGATGAA AAACAAATCG ACTTGTTGTC CATTCCATCG 
TTTCAAACCG CTGGTTCTAT TGCTTATGCT CATGTGTTTT GCTTTATCAC GCTTGGTCAA 
AGAAATTTAT ATGATGTTCT TTACGAAACC CAAAAAGTCG TTAGGGATAT CATTCGAACG 
GAAGGGTATG CAAATACACT GCAAGGACTA AGCCCAAGTA CCGTATTGTT TCAGCCACGA 
AGTAATTCTA TGTACCCGTG CATTGGAGAT GCATTCAATA ACATGGTGCG ATTGGACTTG 
AGTGAGCTGA CAGCTCTGTA TGAGGGGATG TTTGCCAAAG TTCAGGACTT GTTAAAAGAG 
CTATGTTTTG ATATGAATGT GGAAAAACTA TTACCCATTT CGTTACTAAG GTCAATTGGA 
GATGACATAA ACAATAGTAA ATTGGGATAT TCATTCTTTA AAGAGTCGAT AGAGATACGT 
TCATCTCATA GTGTGCTGTT ACGAACAATA CTTAAGAACA GTGAACTTTG TCATCGGTTT 
TTCCCATCGA TGTCTAAGAA GGATCTGACA AAGTTGTTTG GTGGAGTAAG TGATCAGCAA 
AGAAATGAGT GCGACAACTA TAGCAACCAC TACAACGACA ACTCTAATGA TAATGACAAT 
GATGTGTTTC TCAAATTACA TTGGTCTAAA TCTGCTATTA AAAAGTATGA GACAAAGGCA 
TCTATCTTCA ATGAGTTATT GTTTTGTCTA GTGTACATAT CTGCTGGACA ACCAGCCAGA 
GCACAAGAGA TGGTGTATTG GACTTTGCGG AATGGCAAGT ATAAGACTCG CGAATTGTAT 
TTGATGTTTG GAAGGCTGAT GATTTACAGC AGATACGATA AGACTCGTAA TATGAAGTTT 
GCTGAAAAGC CAATCCCCAG GTTTCTTTCT GAGCCGCTTT CCATTTTAGC ACTTCGGTAC 
TATGTTTTGG TTCGACCATT GGAAGCATTG ATGAAGTATG TGACAACCGC TGATAGGTCG 
AAAGTAGCTG TATACTTGGA TTTCATGTTT GTGATTGCTG GCGAACGATT GCAAAGAGAT 
TTACCGTATC GAATTTTTCC AAAGGCCACC TACCAATGCA TTCAAAAACC GTTGGGATTT 
CGAAACTACA GGCACATTGC TCACTATTTT AAAGAAAAAA ACATCGAGGA AGAAATGACG 
AGGGAATCAT ATTTCGATTT ACAGGCTGGA CATACACGAA ACACAGCGCT CTACATCTAT 
GGGCGCACTA TGGACAACTT GCATTATCTG CCGTCGGATT ATTTCGCCAA CTTTTTTCGT 
GCAAGCTATA AGTGGCAGGA ACTATTACAG ATTCGAGATA ACCCGACCCA TGGACTGTTG 
GTGGAAACAA AGCACCCATT CATCAAGCGA GTTGATCAAT TGGAGGAAGC GCTGAATGAG 
AAGCTGGCAA GGTTGGTAGG TGAACAAATG GTGGAGGGGG ACAAGGAAAA GGACAAGACA 
AATGAGGAGA AGAACAAGGA CGAGGTAAAG GCTGAAATGA CACAGCCCGT TGTAAATCAA 
GACTCTCATG ACTTACAAGA CCAATTGGCC ACTACGCCCA CCGCGCCCAC CGCATTTCAC 
TACCGCCCAG GACTTCTTCA ACCATCTCAA ACTTCTGTTC AACATTGTTG TTGGGCATTG 
TCTCAATATT ATGGTCTCGA AGCGAAATTC CGTTCGCTGA AACAATTTCA ATCGGTTTAT 
TTTTCTCTTT TAAATCGTAT GAATTTGATT ACCGTACTCC CAACTGGAGG TGGAAAGTCT 
TTGTCGTTTT TGATACCGGC GCTCATCGAA AAAAAAAGAC AAACCCCAGG AAAGGTGATG 
AATATGGTCA CGCTGGTTCT AGTGCCCATG ATGTCGTTAC GGCAAGATAT GATGCTCAGA 
GTGAATGAAA AGGGACTGCT TGTTTGTTCG GGGAATTGGA CCGCATTCAA AGATGTACGA 
TTGACTTTAG AGACGCAACT TCCCGATTTG TTTATCTTGA CATACGAGTC AGCATTAACC 
AACAGTGGTC TTCGGTTTTT TGAAAGTTTG GCAACACTTG GTCGTTTGGC ACGAGTGGTA 
ATTGATGAAG CACATTTGTT ACTCACCAGT GGAGCATGGA GAACGGCTTT GTCGAGAGCA 
TCGCGGTTGT CTGGCTTGTA TGCACCATTG CACTTGTTGA GTGCCACCTT TCCCAGGCAA 
CTAGAGATGG TTGCCAGACA AACGTTTTGT ACAAACTTTT ACGTTTTACG AGAAACGTCT 
ACTGCACGGG AAAACATCTT TTACTTTTTG CATCCCTATG ATAATACCGA GTTTTTGTTG 
GACTTGCGAA CGTTGATGAA ACGAACAAAG GTCTTTGAAG GTGATGGACG TGCTATCATC 
TTTTGTCGAA CCAAAAAGGA TGTCGAATAC ATTCATCGTC GTCTTCACCA GTCGGACTTG 
TTCGCTCACA CCCATGTAAC CATTTACACA GGAGATGTAA GCGACGAAGA ACGACAAATG 
AACTTTGACG CGTTTCGAAA TGCAAATGGG AAGACACGAA TCATGATCGC TACCAAGGCA 
TTCGGACTCG GTATCAACTA TATGGGAGTG CGTTTAGTAG TACACTATGG ATTACCAGCT 
TCATCTATGG ATTATGTACA GGAGACAGGT CGAGCTGGAA GAGATGGCAA GTATGCGATT 
GCAGCATTGT TTTACGAGAA ATATGATTCT ACATGGTCGA GCTACGTAGA GGATTCGATG 
AAAAACTTTC TTAATGATAA TACGATGTGT GTTCGATCGT TTCTCGCAAG TGAAATGGAT 
GGCGAATGTG TATGTTGTGC ATCGTTTGCT AACTGTGTTT ACTGCTCAAG ATGCTCAGAT 
TCGTTACTTG GTGAAGAATC AACTGTGTCT ACGATGTATG GAGTGAAACC GACATTGCCA 
GAAACACCGA AACCAGCCAT TGCAACACAT TCGCGTTATA ATGCATCGTT TTCGTCTTCC 
CCCCCACCAC AGCCAGGGAA TAGCAGTGGT ATGAGTGCTA TGAACACTAA CACTACTAGT 
ACTACGCCAG TGTCTTTGTC GGAATTATCG GAAATCACTT TGTTCCCTTC GTCTGTATCG 
CCAACATGGA AGAAAAGCTT TGGCAATGCT AATACGAATC TAAAGTATGG TTTGGAAGAC 
ATGTCGCTCA GCCATCGTCG TGGTCATAAA CGCACATACG ATGAACATTT GAACAATGTA 
CAACAGGGCG TCAACCATGA CATGAATCGT GTTCATGGTA GTGTTGGAGG TATGAGTGGT 
ATTGTTGGTA TTGGTATTGG TATTGGTGAT GGTGATGGTG ATGGTGATGT TGATTCTCGT 
ACCATTCATT TCGCCGAGTA TAAAAGTCGT GTGCAAGCCG TCAAAAAACA ATGGGTAGAC 
TCAACTGACA TTTCAGCACA ACTCGAGCGA TTCTTTCGAG TGTATAAAGA TGAATGTCTA 
AGTTGCACTC TTGGTAACCC AGACACAGAA ATTCGTGCTC ATACCGGGAA AGCATGCCCC 
GTACGCTTAT CTACTTGTTA TAAATGTGGC AAGGCCGACC ACAACTTGCG AGAATGTAAA 
CTACGTATTC GATTTCAAGG ACTTTGTTTA TTTTGTGGTT TGACAAAGTT TGAACATGCC 
GACAGTGATA TGGCTTACAC TTCTGACTGT AGGTCATGGG CTAGAAAGGC AAACTTGATA 
TCGTTAGTTT ACTATGCTTG GAACAATGTA CAATACCGAA GGACTATTGC AGATAAATTC 
TTACAAGGTG ATGTACGTGA TCAAGCTTTT TATGGATTTG TGTGTTGCTC TACAACTACG 
AATTCTGCAT TTGTTCTAGT TATTCATTAC TTGTTGTCTG ATGTTTTGCA AATCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343051.1
CDS	1..5760
Translation	MVVASEIAKVASKTARDIAGCFTCQCGTQFDNVERIVQHFKECRYRDETCKDDDIVVYEPSSFVQDEKKDKPIIVEAASEATSEEACNSSKERQLPALSALSALSTLTTSANDDLWTARLIWQSTNDTKLDNSPSSNYTDLNHKLANYGLSILSIHALMCVECECLLNVIHTAQHMQIVHKLELNEDLLWFQELRTLKLKSPTNVLQTHSSQTHVYPYIRGLPVLLNGYECVPCTKNGTGFVHAIMDTFRHHVRRTHGKVIKLENCIRRTALQTVKNKYAQRCQFFKVDYVPLNGGEEEEEEEGEEKEDAQNIKERMVDFCFSKFMEKNQQRREQQDKGENKKRQDDVDQATDNNTNTILEDDEKDNDEEEEEEIVNAREKNLLNQQFNWTAIVKKLGENWDQLVRFEYTNGIVTLDTIVNQLIRYYYRGFRHLSGMTMGMRRMFTQGGSYSAQERGLCRLEQKDTVVRYAQSAALYLIFLLRRPSADSGIRRHLEAMCGATVERKEGGSNSSSNISNVANFDSAEDDNDNDNDNDRDSNNNNNNNNTNTDDDDKLAYLELHEALKLAFLQQYDFSKNVQDLEIMEFLACMSLHKDGTSKYAYEISACFAPLIYTCRLVAACELQRLIDEKQIDLLSIPSFQTAGSIAYAHVFCFITLGQRNLYDVLYETQKVVRDIIRTEGYANTLQGLSPSTVLFQPRSNSMYPCIGDAFNNMVRLDLSELTALYEGMFAKVQDLLKELCFDMNVEKLLPISLLRSIGDDINNSKLGYSFFKESIEIRSSHSVLLRTILKNSELCHRFFPSMSKKDLTKLFGGVSDQQRNECDNYSNHYNDNSNDNDNDVFLKLHWSKSAIKKYETKASIFNELLFCLVYISAGQPARAQEMVYWTLRNGKYKTRELYLMFGRLMIYSRYDKTRNMKFAEKPIPRFLSEPLSILALRYYVLVRPLEALMKYVTTADRSKVAVYLDFMFVIAGERLQRDLPYRIFPKATYQCIQKPLGFRNYRHIAHYFKEKNIEEEMTRESYFDLQAGHTRNTALYIYGRTMDNLHYLPSDYFANFFRASYKWQELLQIRDNPTHGLLVETKHPFIKRVDQLEEALNEKLARLVGEQMVEGDKEKDKTNEEKNKDEVKAEMTQPVVNQDSHDLQDQLATTPTAPTAFHYRPGLLQPSQTSVQHCCWALSQYYGLEAKFRSLKQFQSVYFSLLNRMNLITVLPTGGGKSLSFLIPALIEKKRQTPGKVMNMVTLVLVPMMSLRQDMMLRVNEKGLLVCSGNWTAFKDVRLTLETQLPDLFILTYESALTNSGLRFFESLATLGRLARVVIDEAHLLLTSGAWRTALSRASRLSGLYAPLHLLSATFPRQLEMVARQTFCTNFYVLRETSTARENIFYFLHPYDNTEFLLDLRTLMKRTKVFEGDGRAIIFCRTKKDVEYIHRRLHQSDLFAHTHVTIYTGDVSDEERQMNFDAFRNANGKTRIMIATKAFGLGINYMGVRLVVHYGLPASSMDYVQETGRAGRDGKYAIAALFYEKYDSTWSSYVEDSMKNFLNDNTMCVRSFLASEMDGECVCCASFANCVYCSRCSDSLLGEESTVSTMYGVKPTLPETPKPAIATHSRYNASFSSSPPPQPGNSSGMSAMNTNTTSTTPVSLSELSEITLFPSSVSPTWKKSFGNANTNLKYGLEDMSLSHRRGHKRTYDEHLNNVQQGVNHDMNRVHGSVGGMSGIVGIGIGIGDGDGDGDVDSRTIHFAEYKSRVQAVKKQWVDSTDISAQLERFFRVYKDECLSCTLGNPDTEIRAHTGKACPVRLSTCYKCGKADHNLRECKLRIRFQGLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVYYAWNNVQYRRTIADKFLQGDVRDQAFYGFVCCSTTTNSAFVLVIHYLLSDVLQIM

Target ORF information:

RefSeq Version	NM_001355852.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe RecQ type DNA helicase Tlh1 (tlh2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355852.1

ORF Insert Sequence:

ATGGTCGTCG CTTCAGAAAT TGCTAAAGTC GCTTCAAAAA CTGCTAGAGA TATCGCCGGA 
TGCTTTACTT GTCAATGTGG AACTCAATTT GATAATGTAG AAAGGATCGT TCAACATTTC 
AAGGAATGTC GATACAGAGA CGAAACTTGT AAAGACGATG ATATCGTCGT CTATGAACCC 
AGCTCTTTCG TTCAGGACGA GAAAAAGGAT AAGCCAATCA TCGTTGAGGC TGCCAGTGAG 
GCTACCAGTG AGGAGGCTTG CAATTCTTCC AAGGAGCGTC AACTACCCGC TCTCTCTGCT 
CTTTCTGCTC TCTCTACTCT CACTACGAGT GCAAATGACG ACTTGTGGAC TGCACGGCTA 
ATTTGGCAAA GTACAAATGA CACCAAATTG GACAACAGCC CATCATCAAA TTATACTGAC 
TTGAATCACA AGTTGGCTAA TTATGGACTC TCCATTTTGT CTATTCATGC TTTGATGTGC 
GTAGAATGTG AATGCTTGTT AAACGTGATT CACACTGCGC AACATATGCA AATTGTGCAT 
AAACTAGAGT TAAACGAAGA TCTTTTGTGG TTTCAAGAAC TAAGGACTTT AAAGTTAAAA 
AGTCCAACAA ATGTTTTACA AACTCATTCT TCTCAAACCC ATGTATACCC ATACATCAGA 
GGACTACCAG TACTTTTAAA TGGATATGAG TGTGTGCCGT GCACAAAAAA CGGAACAGGA 
TTCGTACATG CAATCATGGA TACCTTTCGC CATCATGTTC GACGTACTCA TGGAAAAGTT 
ATAAAATTGG AAAATTGTAT ACGTCGAACA GCTTTGCAAA CGGTTAAGAA CAAGTATGCT 
CAACGATGTC AGTTCTTCAA AGTCGATTAT GTACCTTTGA ATGGAGGAGA AGAAGAAGAA 
GAGGAGGAGG GGGAGGAGAA AGAAGATGCA CAAAACATCA AGGAAAGAAT GGTGGACTTT 
TGCTTTTCAA AATTTATGGA GAAGAATCAA CAGCGGCGGG AGCAGCAAGA CAAAGGTGAA 
AATAAAAAAC GACAAGATGA TGTTGACCAA GCAACAGACA ACAACACTAA CACTATTCTT 
GAAGATGATG AAAAAGATAA CGATGAAGAG GAAGAGGAAG AGATTGTCAA TGCACGAGAG 
AAAAATCTTT TAAATCAACA ATTCAATTGG ACGGCAATTG TGAAAAAACT TGGAGAAAAT 
TGGGATCAGC TGGTTCGATT CGAATATACG AATGGAATTG TAACTTTGGA TACCATTGTA 
AACCAGTTGA TTCGTTATTA CTATCGAGGA TTCCGTCATC TAAGTGGAAT GACCATGGGC 
ATGAGACGAA TGTTTACTCA AGGAGGAAGC TATAGTGCAC AAGAGCGAGG ACTTTGTCGC 
TTAGAGCAAA AGGATACAGT TGTGAGATAT GCTCAAAGTG CAGCACTATA CCTAATATTT 
CTGTTACGTC GTCCATCAGC GGACTCGGGG ATAAGAAGAC ACTTGGAAGC AATGTGTGGA 
GCAACAGTGG AGAGAAAAGA AGGTGGCAGT AACAGCAGCA GTAACATCAG CAACGTCGCC 
AACTTTGATA GTGCTGAAGA CGACAATGAC AATGACAATG ACAATGACAG AGACAGTAAC 
AATAACAATA ACAATAACAA CACCAATACT GACGATGATG ATAAGCTGGC ATACTTGGAA 
CTGCACGAGG CATTAAAATT GGCTTTTCTG CAACAATACG ATTTTTCTAA AAATGTTCAA 
GACTTGGAAA TCATGGAGTT TCTTGCATGC ATGTCTTTGC ATAAAGATGG TACTTCAAAA 
TATGCCTATG AAATCTCTGC CTGCTTTGCA CCCCTAATAT ACACGTGTCG TCTTGTAGCA 
GCATGTGAGT TACAACGATT GATCGATGAA AAACAAATCG ACTTGTTGTC CATTCCATCG 
TTTCAAACCG CTGGTTCTAT TGCTTATGCT CATGTGTTTT GCTTTATCAC GCTTGGTCAA 
AGAAATTTAT ATGATGTTCT TTACGAAACC CAAAAAGTCG TTAGGGATAT CATTCGAACG 
GAAGGGTATG CAAATACACT GCAAGGACTA AGCCCAAGTA CCGTATTGTT TCAGCCACGA 
AGTAATTCTA TGTACCCGTG CATTGGAGAT GCATTCAATA ACATGGTGCG ATTGGACTTG 
AGTGAGCTGA CAGCTCTGTA TGAGGGGATG TTTGCCAAAG TTCAGGACTT GTTAAAAGAG 
CTATGTTTTG ATATGAATGT GGAAAAACTA TTACCCATTT CGTTACTAAG GTCAATTGGA 
GATGACATAA ACAATAGTAA ATTGGGATAT TCATTCTTTA AAGAGTCGAT AGAGATACGT 
TCATCTCATA GTGTGCTGTT ACGAACAATA CTTAAGAACA GTGAACTTTG TCATCGGTTT 
TTCCCATCGA TGTCTAAGAA GGATCTGACA AAGTTGTTTG GTGGAGTAAG TGATCAGCAA 
AGAAATGAGT GCGACAACTA TAGCAACCAC TACAACGACA ACTCTAATGA TAATGACAAT 
GATGTGTTTC TCAAATTACA TTGGTCTAAA TCTGCTATTA AAAAGTATGA GACAAAGGCA 
TCTATCTTCA ATGAGTTATT GTTTTGTCTA GTGTACATAT CTGCTGGACA ACCAGCCAGA 
GCACAAGAGA TGGTGTATTG GACTTTGCGG AATGGCAAGT ATAAGACTCG CGAATTGTAT 
TTGATGTTTG GAAGGCTGAT GATTTACAGC AGATACGATA AGACTCGTAA TATGAAGTTT 
GCTGAAAAGC CAATCCCCAG GTTTCTTTCT GAGCCGCTTT CCATTTTAGC ACTTCGGTAC 
TATGTTTTGG TTCGACCATT GGAAGCATTG ATGAAGTATG TGACAACCGC TGATAGGTCG 
AAAGTAGCTG TATACTTGGA TTTCATGTTT GTGATTGCTG GCGAACGATT GCAAAGAGAT 
TTACCGTATC GAATTTTTCC AAAGGCCACC TACCAATGCA TTCAAAAACC GTTGGGATTT 
CGAAACTACA GGCACATTGC TCACTATTTT AAAGAAAAAA ACATCGAGGA AGAAATGACG 
AGGGAATCAT ATTTCGATTT ACAGGCTGGA CATACACGAA ACACAGCGCT CTACATCTAT 
GGGCGCACTA TGGACAACTT GCATTATCTG CCGTCGGATT ATTTCGCCAA CTTTTTTCGT 
GCAAGCTATA AGTGGCAGGA ACTATTACAG ATTCGAGATA ACCCGACCCA TGGACTGTTG 
GTGGAAACAA AGCACCCATT CATCAAGCGA GTTGATCAAT TGGAGGAAGC GCTGAATGAG 
AAGCTGGCAA GGTTGGTAGG TGAACAAATG GTGGAGGGGG ACAAGGAAAA GGACAAGACA 
AATGAGGAGA AGAACAAGGA CGAGGTAAAG GCTGAAATGA CACAGCCCGT TGTAAATCAA 
GACTCTCATG ACTTACAAGA CCAATTGGCC ACTACGCCCA CCGCGCCCAC CGCATTTCAC 
TACCGCCCAG GACTTCTTCA ACCATCTCAA ACTTCTGTTC AACATTGTTG TTGGGCATTG 
TCTCAATATT ATGGTCTCGA AGCGAAATTC CGTTCGCTGA AACAATTTCA ATCGGTTTAT 
TTTTCTCTTT TAAATCGTAT GAATTTGATT ACCGTACTCC CAACTGGAGG TGGAAAGTCT 
TTGTCGTTTT TGATACCGGC GCTCATCGAA AAAAAAAGAC AAACCCCAGG AAAGGTGATG 
AATATGGTCA CGCTGGTTCT AGTGCCCATG ATGTCGTTAC GGCAAGATAT GATGCTCAGA 
GTGAATGAAA AGGGACTGCT TGTTTGTTCG GGGAATTGGA CCGCATTCAA AGATGTACGA 
TTGACTTTAG AGACGCAACT TCCCGATTTG TTTATCTTGA CATACGAGTC AGCATTAACC 
AACAGTGGTC TTCGGTTTTT TGAAAGTTTG GCAACACTTG GTCGTTTGGC ACGAGTGGTA 
ATTGATGAAG CACATTTGTT ACTCACCAGT GGAGCATGGA GAACGGCTTT GTCGAGAGCA 
TCGCGGTTGT CTGGCTTGTA TGCACCATTG CACTTGTTGA GTGCCACCTT TCCCAGGCAA 
CTAGAGATGG TTGCCAGACA AACGTTTTGT ACAAACTTTT ACGTTTTACG AGAAACGTCT 
ACTGCACGGG AAAACATCTT TTACTTTTTG CATCCCTATG ATAATACCGA GTTTTTGTTG 
GACTTGCGAA CGTTGATGAA ACGAACAAAG GTCTTTGAAG GTGATGGACG TGCTATCATC 
TTTTGTCGAA CCAAAAAGGA TGTCGAATAC ATTCATCGTC GTCTTCACCA GTCGGACTTG 
TTCGCTCACA CCCATGTAAC CATTTACACA GGAGATGTAA GCGACGAAGA ACGACAAATG 
AACTTTGACG CGTTTCGAAA TGCAAATGGG AAGACACGAA TCATGATCGC TACCAAGGCA 
TTCGGACTCG GTATCAACTA TATGGGAGTG CGTTTAGTAG TACACTATGG ATTACCAGCT 
TCATCTATGG ATTATGTACA GGAGACAGGT CGAGCTGGAA GAGATGGCAA GTATGCGATT 
GCAGCATTGT TTTACGAGAA ATATGATTCT ACATGGTCGA GCTACGTAGA GGATTCGATG 
AAAAACTTTC TTAATGATAA TACGATGTGT GTTCGATCGT TTCTCGCAAG TGAAATGGAT 
GGCGAATGTG TATGTTGTGC ATCGTTTGCT AACTGTGTTT ACTGCTCAAG ATGCTCAGAT 
TCGTTACTTG GTGAAGAATC AACTGTGTCT ACGATGTATG GAGTGAAACC GACATTGCCA 
GAAACACCGA AACCAGCCAT TGCAACACAT TCGCGTTATA ATGCATCGTT TTCGTCTTCC 
CCCCCACCAC AGCCAGGGAA TAGCAGTGGT ATGAGTGCTA TGAACACTAA CACTACTAGT 
ACTACGCCAG TGTCTTTGTC GGAATTATCG GAAATCACTT TGTTCCCTTC GTCTGTATCG 
CCAACATGGA AGAAAAGCTT TGGCAATGCT AATACGAATC TAAAGTATGG TTTGGAAGAC 
ATGTCGCTCA GCCATCGTCG TGGTCATAAA CGCACATACG ATGAACATTT GAACAATGTA 
CAACAGGGCG TCAACCATGA CATGAATCGT GTTCATGGTA GTGTTGGAGG TATGAGTGGT 
ATTGTTGGTA TTGGTATTGG TATTGGTGAT GGTGATGGTG ATGGTGATGT TGATTCTCGT 
ACCATTCATT TCGCCGAGTA TAAAAGTCGT GTGCAAGCCG TCAAAAAACA ATGGGTAGAC 
TCAACTGACA TTTCAGCACA ACTCGAGCGA TTCTTTCGAG TGTATAAAGA TGAATGTCTA 
AGTTGCACTC TTGGTAACCC AGACACAGAA ATTCGTGCTC ATACCGGGAA AGCATGCCCC 
GTACGCTTAT CTACTTGTTA TAAATGTGGC AAGGCCGACC ACAACTTGCG AGAATGTAAA 
CTACGTATTC GATTTCAAGG ACTTTGTTTA TTTTGTGGTT TGACAAAGTT TGAACATGCC 
GACAGTGATA TGGCTTACAC TTCTGACTGT AGGTCATGGG CTAGAAAGGC AAACTTGATA 
TCGTTAGTTT ACTATGCTTG GAACAATGTA CAATACCGAA GGACTATTGC AGATAAATTC 
TTACAAGGTG ATGTACGTGA TCAAGCTTTT TATGGATTTG TGTGTTGCTC TACAACTACG 
AATTCTGCAT TTGTTCTAGT TATTCATTAC TTGTTGTCTG ATGTTTTGCA AATCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tlh2 ( XM_001713106.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> XP_001713158.1 Schizosaccharomyces pombe 972h- RecQ type DNA helicase Tlh1 (tlh2), mRNA.

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CloneID	OSj04973
Clone ID Related Accession (Same CDS sequence)	XM_001713106.2
Accession Version	XM_001713106.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	RecQ type DNA helicase Tlh1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced XM_001713106.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGGTCGTCG CTTCAGAAAT TGCTAAAGTC GCTTCAAAAA CTGCTAGAGA TATCGCCGGA 
TGCTTTACTT GTCAATGTGG AACTCAATTT GATAATGTAG AAAGGATCGT TCAACATTTC 
AAGGAATGTC GATACAGAGA CGAAACTTGT AAAGACGATG ATATCGTCGT CTATGAACCC 
AGCTCTTTCG TTCAGGACGA GAAAAAGGAT AAGCCAATCA TCGTTGAGGC TGCCAGTGAG 
GCTACCAGTG AGGAGGCTTG CAATTCTTCC AAGGAGCGTC AACTACCCGC TCTCTCTGCT 
CTTTCTGCTC TCTCTACTCT CACTACGAGT GCAAATGACG ACTTGTGGAC TGCACGGCTA 
ATTTGGCAAA GTACAAATGA CACCAAATTG GACAACAGCC CATCATCAAA TTATACTGAC 
TTGAATCACA AGTTGGCTAA TTATGGACTC TCCATTTTGT CTATTCATGC TTTGATGTGC 
GTAGAATGTG AATGCTTGTT AAACGTGATT CACACTGCGC AACATATGCA AATTGTGCAT 
AAACTAGAGT TAAACGAAGA TCTTTTGTGG TTTCAAGAAC TAAGGACTTT AAAGTTAAAA 
AGTCCAACAA ATGTTTTACA AACTCATTCT TCTCAAACCC ATGTATACCC ATACATCAGA 
GGACTACCAG TACTTTTAAA TGGATATGAG TGTGTGCCGT GCACAAAAAA CGGAACAGGA 
TTCGTACATG CAATCATGGA TACCTTTCGC CATCATGTTC GACGTACTCA TGGAAAAGTT 
ATAAAATTGG AAAATTGTAT ACGTCGAACA GCTTTGCAAA CGGTTAAGAA CAAGTATGCT 
CAACGATGTC AGTTCTTCAA AGTCGATTAT GTACCTTTGA ATGGAGGAGA AGAAGAAGAA 
GAGGAGGAGG GGGAGGAGAA AGAAGATGCA CAAAACATCA AGGAAAGAAT GGTGGACTTT 
TGCTTTTCAA AATTTATGGA GAAGAATCAA CAGCGGCGGG AGCAGCAAGA CAAAGGTGAA 
AATAAAAAAC GACAAGATGA TGTTGACCAA GCAACAGACA ACAACACTAA CACTATTCTT 
GAAGATGATG AAAAAGATAA CGATGAAGAG GAAGAGGAAG AGATTGTCAA TGCACGAGAG 
AAAAATCTTT TAAATCAACA ATTCAATTGG ACGGCAATTG TGAAAAAACT TGGAGAAAAT 
TGGGATCAGC TGGTTCGATT CGAATATACG AATGGAATTG TAACTTTGGA TACCATTGTA 
AACCAGTTGA TTCGTTATTA CTATCGAGGA TTCCGTCATC TAAGTGGAAT GACCATGGGC 
ATGAGACGAA TGTTTACTCA AGGAGGAAGC TATAGTGCAC AAGAGCGAGG ACTTTGTCGC 
TTAGAGCAAA AGGATACAGT TGTGAGATAT GCTCAAAGTG CAGCACTATA CCTAATATTT 
CTGTTACGTC GTCCATCAGC GGACTCGGGG ATAAGAAGAC ACTTGGAAGC AATGTGTGGA 
GCAACAGTGG AGAGAAAAGA AGGTGGCAGT AACAGCAGCA GTAACATCAG CAACGTCGCC 
AACTTTGATA GTGCTGAAGA CGACAATGAC AATGACAATG ACAATGACAG AGACAGTAAC 
AATAACAATA ACAATAACAA CACCAATACT GACGATGATG ATAAGCTGGC ATACTTGGAA 
CTGCACGAGG CATTAAAATT GGCTTTTCTG CAACAATACG ATTTTTCTAA AAATGTTCAA 
GACTTGGAAA TCATGGAGTT TCTTGCATGC ATGTCTTTGC ATAAAGATGG TACTTCAAAA 
TATGCCTATG AAATCTCTGC CTGCTTTGCA CCCCTAATAT ACACGTGTCG TCTTGTAGCA 
GCATGTGAGT TACAACGATT GATCGATGAA AAACAAATCG ACTTGTTGTC CATTCCATCG 
TTTCAAACCG CTGGTTCTAT TGCTTATGCT CATGTGTTTT GCTTTATCAC GCTTGGTCAA 
AGAAATTTAT ATGATGTTCT TTACGAAACC CAAAAAGTCG TTAGGGATAT CATTCGAACG 
GAAGGGTATG CAAATACACT GCAAGGACTA AGCCCAAGTA CCGTATTGTT TCAGCCACGA 
AGTAATTCTA TGTACCCGTG CATTGGAGAT GCATTCAATA ACATGGTGCG ATTGGACTTG 
AGTGAGCTGA CAGCTCTGTA TGAGGGGATG TTTGCCAAAG TTCAGGACTT GTTAAAAGAG 
CTATGTTTTG ATATGAATGT GGAAAAACTA TTACCCATTT CGTTACTAAG GTCAATTGGA 
GATGACATAA ACAATAGTAA ATTGGGATAT TCATTCTTTA AAGAGTCGAT AGAGATACGT 
TCATCTCATA GTGTGCTGTT ACGAACAATA CTTAAGAACA GTGAACTTTG TCATCGGTTT 
TTCCCATCGA TGTCTAAGAA GGATCTGACA AAGTTGTTTG GTGGAGTAAG TGATCAGCAA 
AGAAATGAGT GCGACAACTA TAGCAACCAC TACAACGACA ACTCTAATGA TAATGACAAT 
GATGTGTTTC TCAAATTACA TTGGTCTAAA TCTGCTATTA AAAAGTATGA GACAAAGGCA 
TCTATCTTCA ATGAGTTATT GTTTTGTCTA GTGTACATAT CTGCTGGACA ACCAGCCAGA 
GCACAAGAGA TGGTGTATTG GACTTTGCGG AATGGCAAGT ATAAGACTCG CGAATTGTAT 
TTGATGTTTG GAAGGCTGAT GATTTACAGC AGATACGATA AGACTCGTAA TATGAAGTTT 
GCTGAAAAGC CAATCCCCAG GTTTCTTTCT GAGCCGCTTT CCATTTTAGC ACTTCGGTAC 
TATGTTTTGG TTCGACCATT GGAAGCATTG ATGAAGTATG TGACAACCGC TGATAGGTCG 
AAAGTAGCTG TATACTTGGA TTTCATGTTT GTGATTGCTG GCGAACGATT GCAAAGAGAT 
TTACCGTATC GAATTTTTCC AAAGGCCACC TACCAATGCA TTCAAAAACC GTTGGGATTT 
CGAAACTACA GGCACATTGC TCACTATTTT AAAGAAAAAA ACATCGAGGA AGAAATGACG 
AGGGAATCAT ATTTCGATTT ACAGGCTGGA CATACACGAA ACACAGCGCT CTACATCTAT 
GGGCGCACTA TGGACAACTT GCATTATCTG CCGTCGGATT ATTTCGCCAA CTTTTTTCGT 
GCAAGCTATA AGTGGCAGGA ACTATTACAG ATTCGAGATA ACCCGACCCA TGGACTGTTG 
GTGGAAACAA AGCACCCATT CATCAAGCGA GTTGATCAAT TGGAGGAAGC GCTGAATGAG 
AAGCTGGCAA GGTTGGTAGG TGAACAAATG GTGGAGGGGG ACAAGGAAAA GGACAAGACA 
AATGAGGAGA AGAACAAGGA CGAGGTAAAG GCTGAAATGA CACAGCCCGT TGTAAATCAA 
GACTCTCATG ACTTACAAGA CCAATTGGCC ACTACGCCCA CCGCGCCCAC CGCATTTCAC 
TACCGCCCAG GACTTCTTCA ACCATCTCAA ACTTCTGTTC AACATTGTTG TTGGGCATTG 
TCTCAATATT ATGGTCTCGA AGCGAAATTC CGTTCGCTGA AACAATTTCA ATCGGTTTAT 
TTTTCTCTTT TAAATCGTAT GAATTTGATT ACCGTACTCC CAACTGGAGG TGGAAAGTCT 
TTGTCGTTTT TGATACCGGC GCTCATCGAA AAAAAAAGAC AAACCCCAGG AAAGGTGATG 
AATATGGTCA CGCTGGTTCT AGTGCCCATG ATGTCGTTAC GGCAAGATAT GATGCTCAGA 
GTGAATGAAA AGGGACTGCT TGTTTGTTCG GGGAATTGGA CCGCATTCAA AGATGTACGA 
TTGACTTTAG AGACGCAACT TCCCGATTTG TTTATCTTGA CATACGAGTC AGCATTAACC 
AACAGTGGTC TTCGGTTTTT TGAAAGTTTG GCAACACTTG GTCGTTTGGC ACGAGTGGTA 
ATTGATGAAG CACATTTGTT ACTCACCAGT GGAGCATGGA GAACGGCTTT GTCGAGAGCA 
TCGCGGTTGT CTGGCTTGTA TGCACCATTG CACTTGTTGA GTGCCACCTT TCCCAGGCAA 
CTAGAGATGG TTGCCAGACA AACGTTTTGT ACAAACTTTT ACGTTTTACG AGAAACGTCT 
ACTGCACGGG AAAACATCTT TTACTTTTTG CATCCCTATG ATAATACCGA GTTTTTGTTG 
GACTTGCGAA CGTTGATGAA ACGAACAAAG GTCTTTGAAG GTGATGGACG TGCTATCATC 
TTTTGTCGAA CCAAAAAGGA TGTCGAATAC ATTCATCGTC GTCTTCACCA GTCGGACTTG 
TTCGCTCACA CCCATGTAAC CATTTACACA GGAGATGTAA GCGACGAAGA ACGACAAATG 
AACTTTGACG CGTTTCGAAA TGCAAATGGG AAGACACGAA TCATGATCGC TACCAAGGCA 
TTCGGACTCG GTATCAACTA TATGGGAGTG CGTTTAGTAG TACACTATGG ATTACCAGCT 
TCATCTATGG ATTATGTACA GGAGACAGGT CGAGCTGGAA GAGATGGCAA GTATGCGATT 
GCAGCATTGT TTTACGAGAA ATATGATTCT ACATGGTCGA GCTACGTAGA GGATTCGATG 
AAAAACTTTC TTAATGATAA TACGATGTGT GTTCGATCGT TTCTCGCAAG TGAAATGGAT 
GGCGAATGTG TATGTTGTGC ATCGTTTGCT AACTGTGTTT ACTGCTCAAG ATGCTCAGAT 
TCGTTACTTG GTGAAGAATC AACTGTGTCT ACGATGTATG GAGTGAAACC GACATTGCCA 
GAAACACCGA AACCAGCCAT TGCAACACAT TCGCGTTATA ATGCATCGTT TTCGTCTTCC 
CCCCCACCAC AGCCAGGGAA TAGCAGTGGT ATGAGTGCTA TGAACACTAA CACTACTAGT 
ACTACGCCAG TGTCTTTGTC GGAATTATCG GAAATCACTT TGTTCCCTTC GTCTGTATCG 
CCAACATGGA AGAAAAGCTT TGGCAATGCT AATACGAATC TAAAGTATGG TTTGGAAGAC 
ATGTCGCTCA GCCATCGTCG TGGTCATAAA CGCACATACG ATGAACATTT GAACAATGTA 
CAACAGGGCG TCAACCATGA CATGAATCGT GTTCATGGTA GTGTTGGAGG TATGAGTGGT 
ATTGTTGGTA TTGGTATTGG TATTGGTGAT GGTGATGGTG ATGGTGATGT TGATTCTCGT 
ACCATTCATT TCGCCGAGTA TAAAAGTCGT GTGCAAGCCG TCAAAAAACA ATGGGTAGAC 
TCAACTGACA TTTCAGCACA ACTCGAGCGA TTCTTTCGAG TGTATAAAGA TGAATGTCTA 
AGTTGCACTC TTGGTAACCC AGACACAGAA ATTCGTGCTC ATACCGGGAA AGCATGCCCC 
GTACGCTTAT CTACTTGTTA TAAATGTGGC AAGGCCGACC ACAACTTGCG AGAATGTAAA 
CTACGTATTC GATTTCAAGG ACTTTGTTTA TTTTGTGGTT TGACAAAGTT TGAACATGCC 
GACAGTGATA TGGCTTACAC TTCTGACTGT AGGTCATGGG CTAGAAAGGC AAACTTGATA 
TCGTTAGTTT ACTATGCTTG GAACAATGTA CAATACCGAA GGACTATTGC AGATAAATTC 
TTACAAGGTG ATGTACGTGA TCAAGCTTTT TATGGATTTG TGTGTTGCTC TACAACTACG 
AATTCTGCAT TTGTTCTAGT TATTCATTAC TTGTTGTCTG ATGTTTTGCA AATCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713158.1
CDS	1..5760
Translation	MVVASEIAKVASKTARDIAGCFTCQCGTQFDNVERIVQHFKECRYRDETCKDDDIVVYEPSSFVQDEKKDKPIIVEAASEATSEEACNSSKERQLPALSALSALSTLTTSANDDLWTARLIWQSTNDTKLDNSPSSNYTDLNHKLANYGLSILSIHALMCVECECLLNVIHTAQHMQIVHKLELNEDLLWFQELRTLKLKSPTNVLQTHSSQTHVYPYIRGLPVLLNGYECVPCTKNGTGFVHAIMDTFRHHVRRTHGKVIKLENCIRRTALQTVKNKYAQRCQFFKVDYVPLNGGEEEEEEEGEEKEDAQNIKERMVDFCFSKFMEKNQQRREQQDKGENKKRQDDVDQATDNNTNTILEDDEKDNDEEEEEEIVNAREKNLLNQQFNWTAIVKKLGENWDQLVRFEYTNGIVTLDTIVNQLIRYYYRGFRHLSGMTMGMRRMFTQGGSYSAQERGLCRLEQKDTVVRYAQSAALYLIFLLRRPSADSGIRRHLEAMCGATVERKEGGSNSSSNISNVANFDSAEDDNDNDNDNDRDSNNNNNNNNTNTDDDDKLAYLELHEALKLAFLQQYDFSKNVQDLEIMEFLACMSLHKDGTSKYAYEISACFAPLIYTCRLVAACELQRLIDEKQIDLLSIPSFQTAGSIAYAHVFCFITLGQRNLYDVLYETQKVVRDIIRTEGYANTLQGLSPSTVLFQPRSNSMYPCIGDAFNNMVRLDLSELTALYEGMFAKVQDLLKELCFDMNVEKLLPISLLRSIGDDINNSKLGYSFFKESIEIRSSHSVLLRTILKNSELCHRFFPSMSKKDLTKLFGGVSDQQRNECDNYSNHYNDNSNDNDNDVFLKLHWSKSAIKKYETKASIFNELLFCLVYISAGQPARAQEMVYWTLRNGKYKTRELYLMFGRLMIYSRYDKTRNMKFAEKPIPRFLSEPLSILALRYYVLVRPLEALMKYVTTADRSKVAVYLDFMFVIAGERLQRDLPYRIFPKATYQCIQKPLGFRNYRHIAHYFKEKNIEEEMTRESYFDLQAGHTRNTALYIYGRTMDNLHYLPSDYFANFFRASYKWQELLQIRDNPTHGLLVETKHPFIKRVDQLEEALNEKLARLVGEQMVEGDKEKDKTNEEKNKDEVKAEMTQPVVNQDSHDLQDQLATTPTAPTAFHYRPGLLQPSQTSVQHCCWALSQYYGLEAKFRSLKQFQSVYFSLLNRMNLITVLPTGGGKSLSFLIPALIEKKRQTPGKVMNMVTLVLVPMMSLRQDMMLRVNEKGLLVCSGNWTAFKDVRLTLETQLPDLFILTYESALTNSGLRFFESLATLGRLARVVIDEAHLLLTSGAWRTALSRASRLSGLYAPLHLLSATFPRQLEMVARQTFCTNFYVLRETSTARENIFYFLHPYDNTEFLLDLRTLMKRTKVFEGDGRAIIFCRTKKDVEYIHRRLHQSDLFAHTHVTIYTGDVSDEERQMNFDAFRNANGKTRIMIATKAFGLGINYMGVRLVVHYGLPASSMDYVQETGRAGRDGKYAIAALFYEKYDSTWSSYVEDSMKNFLNDNTMCVRSFLASEMDGECVCCASFANCVYCSRCSDSLLGEESTVSTMYGVKPTLPETPKPAIATHSRYNASFSSSPPPQPGNSSGMSAMNTNTTSTTPVSLSELSEITLFPSSVSPTWKKSFGNANTNLKYGLEDMSLSHRRGHKRTYDEHLNNVQQGVNHDMNRVHGSVGGMSGIVGIGIGIGDGDGDGDVDSRTIHFAEYKSRVQAVKKQWVDSTDISAQLERFFRVYKDECLSCTLGNPDTEIRAHTGKACPVRLSTCYKCGKADHNLRECKLRIRFQGLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVYYAWNNVQYRRTIADKFLQGDVRDQAFYGFVCCSTTTNSAFVLVIHYLLSDVLQIM

Target ORF information:

RefSeq Version	XM_001713106.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- RecQ type DNA helicase Tlh1 (tlh2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001713106.2

ORF Insert Sequence:

ATGGTCGTCG CTTCAGAAAT TGCTAAAGTC GCTTCAAAAA CTGCTAGAGA TATCGCCGGA 
TGCTTTACTT GTCAATGTGG AACTCAATTT GATAATGTAG AAAGGATCGT TCAACATTTC 
AAGGAATGTC GATACAGAGA CGAAACTTGT AAAGACGATG ATATCGTCGT CTATGAACCC 
AGCTCTTTCG TTCAGGACGA GAAAAAGGAT AAGCCAATCA TCGTTGAGGC TGCCAGTGAG 
GCTACCAGTG AGGAGGCTTG CAATTCTTCC AAGGAGCGTC AACTACCCGC TCTCTCTGCT 
CTTTCTGCTC TCTCTACTCT CACTACGAGT GCAAATGACG ACTTGTGGAC TGCACGGCTA 
ATTTGGCAAA GTACAAATGA CACCAAATTG GACAACAGCC CATCATCAAA TTATACTGAC 
TTGAATCACA AGTTGGCTAA TTATGGACTC TCCATTTTGT CTATTCATGC TTTGATGTGC 
GTAGAATGTG AATGCTTGTT AAACGTGATT CACACTGCGC AACATATGCA AATTGTGCAT 
AAACTAGAGT TAAACGAAGA TCTTTTGTGG TTTCAAGAAC TAAGGACTTT AAAGTTAAAA 
AGTCCAACAA ATGTTTTACA AACTCATTCT TCTCAAACCC ATGTATACCC ATACATCAGA 
GGACTACCAG TACTTTTAAA TGGATATGAG TGTGTGCCGT GCACAAAAAA CGGAACAGGA 
TTCGTACATG CAATCATGGA TACCTTTCGC CATCATGTTC GACGTACTCA TGGAAAAGTT 
ATAAAATTGG AAAATTGTAT ACGTCGAACA GCTTTGCAAA CGGTTAAGAA CAAGTATGCT 
CAACGATGTC AGTTCTTCAA AGTCGATTAT GTACCTTTGA ATGGAGGAGA AGAAGAAGAA 
GAGGAGGAGG GGGAGGAGAA AGAAGATGCA CAAAACATCA AGGAAAGAAT GGTGGACTTT 
TGCTTTTCAA AATTTATGGA GAAGAATCAA CAGCGGCGGG AGCAGCAAGA CAAAGGTGAA 
AATAAAAAAC GACAAGATGA TGTTGACCAA GCAACAGACA ACAACACTAA CACTATTCTT 
GAAGATGATG AAAAAGATAA CGATGAAGAG GAAGAGGAAG AGATTGTCAA TGCACGAGAG 
AAAAATCTTT TAAATCAACA ATTCAATTGG ACGGCAATTG TGAAAAAACT TGGAGAAAAT 
TGGGATCAGC TGGTTCGATT CGAATATACG AATGGAATTG TAACTTTGGA TACCATTGTA 
AACCAGTTGA TTCGTTATTA CTATCGAGGA TTCCGTCATC TAAGTGGAAT GACCATGGGC 
ATGAGACGAA TGTTTACTCA AGGAGGAAGC TATAGTGCAC AAGAGCGAGG ACTTTGTCGC 
TTAGAGCAAA AGGATACAGT TGTGAGATAT GCTCAAAGTG CAGCACTATA CCTAATATTT 
CTGTTACGTC GTCCATCAGC GGACTCGGGG ATAAGAAGAC ACTTGGAAGC AATGTGTGGA 
GCAACAGTGG AGAGAAAAGA AGGTGGCAGT AACAGCAGCA GTAACATCAG CAACGTCGCC 
AACTTTGATA GTGCTGAAGA CGACAATGAC AATGACAATG ACAATGACAG AGACAGTAAC 
AATAACAATA ACAATAACAA CACCAATACT GACGATGATG ATAAGCTGGC ATACTTGGAA 
CTGCACGAGG CATTAAAATT GGCTTTTCTG CAACAATACG ATTTTTCTAA AAATGTTCAA 
GACTTGGAAA TCATGGAGTT TCTTGCATGC ATGTCTTTGC ATAAAGATGG TACTTCAAAA 
TATGCCTATG AAATCTCTGC CTGCTTTGCA CCCCTAATAT ACACGTGTCG TCTTGTAGCA 
GCATGTGAGT TACAACGATT GATCGATGAA AAACAAATCG ACTTGTTGTC CATTCCATCG 
TTTCAAACCG CTGGTTCTAT TGCTTATGCT CATGTGTTTT GCTTTATCAC GCTTGGTCAA 
AGAAATTTAT ATGATGTTCT TTACGAAACC CAAAAAGTCG TTAGGGATAT CATTCGAACG 
GAAGGGTATG CAAATACACT GCAAGGACTA AGCCCAAGTA CCGTATTGTT TCAGCCACGA 
AGTAATTCTA TGTACCCGTG CATTGGAGAT GCATTCAATA ACATGGTGCG ATTGGACTTG 
AGTGAGCTGA CAGCTCTGTA TGAGGGGATG TTTGCCAAAG TTCAGGACTT GTTAAAAGAG 
CTATGTTTTG ATATGAATGT GGAAAAACTA TTACCCATTT CGTTACTAAG GTCAATTGGA 
GATGACATAA ACAATAGTAA ATTGGGATAT TCATTCTTTA AAGAGTCGAT AGAGATACGT 
TCATCTCATA GTGTGCTGTT ACGAACAATA CTTAAGAACA GTGAACTTTG TCATCGGTTT 
TTCCCATCGA TGTCTAAGAA GGATCTGACA AAGTTGTTTG GTGGAGTAAG TGATCAGCAA 
AGAAATGAGT GCGACAACTA TAGCAACCAC TACAACGACA ACTCTAATGA TAATGACAAT 
GATGTGTTTC TCAAATTACA TTGGTCTAAA TCTGCTATTA AAAAGTATGA GACAAAGGCA 
TCTATCTTCA ATGAGTTATT GTTTTGTCTA GTGTACATAT CTGCTGGACA ACCAGCCAGA 
GCACAAGAGA TGGTGTATTG GACTTTGCGG AATGGCAAGT ATAAGACTCG CGAATTGTAT 
TTGATGTTTG GAAGGCTGAT GATTTACAGC AGATACGATA AGACTCGTAA TATGAAGTTT 
GCTGAAAAGC CAATCCCCAG GTTTCTTTCT GAGCCGCTTT CCATTTTAGC ACTTCGGTAC 
TATGTTTTGG TTCGACCATT GGAAGCATTG ATGAAGTATG TGACAACCGC TGATAGGTCG 
AAAGTAGCTG TATACTTGGA TTTCATGTTT GTGATTGCTG GCGAACGATT GCAAAGAGAT 
TTACCGTATC GAATTTTTCC AAAGGCCACC TACCAATGCA TTCAAAAACC GTTGGGATTT 
CGAAACTACA GGCACATTGC TCACTATTTT AAAGAAAAAA ACATCGAGGA AGAAATGACG 
AGGGAATCAT ATTTCGATTT ACAGGCTGGA CATACACGAA ACACAGCGCT CTACATCTAT 
GGGCGCACTA TGGACAACTT GCATTATCTG CCGTCGGATT ATTTCGCCAA CTTTTTTCGT 
GCAAGCTATA AGTGGCAGGA ACTATTACAG ATTCGAGATA ACCCGACCCA TGGACTGTTG 
GTGGAAACAA AGCACCCATT CATCAAGCGA GTTGATCAAT TGGAGGAAGC GCTGAATGAG 
AAGCTGGCAA GGTTGGTAGG TGAACAAATG GTGGAGGGGG ACAAGGAAAA GGACAAGACA 
AATGAGGAGA AGAACAAGGA CGAGGTAAAG GCTGAAATGA CACAGCCCGT TGTAAATCAA 
GACTCTCATG ACTTACAAGA CCAATTGGCC ACTACGCCCA CCGCGCCCAC CGCATTTCAC 
TACCGCCCAG GACTTCTTCA ACCATCTCAA ACTTCTGTTC AACATTGTTG TTGGGCATTG 
TCTCAATATT ATGGTCTCGA AGCGAAATTC CGTTCGCTGA AACAATTTCA ATCGGTTTAT 
TTTTCTCTTT TAAATCGTAT GAATTTGATT ACCGTACTCC CAACTGGAGG TGGAAAGTCT 
TTGTCGTTTT TGATACCGGC GCTCATCGAA AAAAAAAGAC AAACCCCAGG AAAGGTGATG 
AATATGGTCA CGCTGGTTCT AGTGCCCATG ATGTCGTTAC GGCAAGATAT GATGCTCAGA 
GTGAATGAAA AGGGACTGCT TGTTTGTTCG GGGAATTGGA CCGCATTCAA AGATGTACGA 
TTGACTTTAG AGACGCAACT TCCCGATTTG TTTATCTTGA CATACGAGTC AGCATTAACC 
AACAGTGGTC TTCGGTTTTT TGAAAGTTTG GCAACACTTG GTCGTTTGGC ACGAGTGGTA 
ATTGATGAAG CACATTTGTT ACTCACCAGT GGAGCATGGA GAACGGCTTT GTCGAGAGCA 
TCGCGGTTGT CTGGCTTGTA TGCACCATTG CACTTGTTGA GTGCCACCTT TCCCAGGCAA 
CTAGAGATGG TTGCCAGACA AACGTTTTGT ACAAACTTTT ACGTTTTACG AGAAACGTCT 
ACTGCACGGG AAAACATCTT TTACTTTTTG CATCCCTATG ATAATACCGA GTTTTTGTTG 
GACTTGCGAA CGTTGATGAA ACGAACAAAG GTCTTTGAAG GTGATGGACG TGCTATCATC 
TTTTGTCGAA CCAAAAAGGA TGTCGAATAC ATTCATCGTC GTCTTCACCA GTCGGACTTG 
TTCGCTCACA CCCATGTAAC CATTTACACA GGAGATGTAA GCGACGAAGA ACGACAAATG 
AACTTTGACG CGTTTCGAAA TGCAAATGGG AAGACACGAA TCATGATCGC TACCAAGGCA 
TTCGGACTCG GTATCAACTA TATGGGAGTG CGTTTAGTAG TACACTATGG ATTACCAGCT 
TCATCTATGG ATTATGTACA GGAGACAGGT CGAGCTGGAA GAGATGGCAA GTATGCGATT 
GCAGCATTGT TTTACGAGAA ATATGATTCT ACATGGTCGA GCTACGTAGA GGATTCGATG 
AAAAACTTTC TTAATGATAA TACGATGTGT GTTCGATCGT TTCTCGCAAG TGAAATGGAT 
GGCGAATGTG TATGTTGTGC ATCGTTTGCT AACTGTGTTT ACTGCTCAAG ATGCTCAGAT 
TCGTTACTTG GTGAAGAATC AACTGTGTCT ACGATGTATG GAGTGAAACC GACATTGCCA 
GAAACACCGA AACCAGCCAT TGCAACACAT TCGCGTTATA ATGCATCGTT TTCGTCTTCC 
CCCCCACCAC AGCCAGGGAA TAGCAGTGGT ATGAGTGCTA TGAACACTAA CACTACTAGT 
ACTACGCCAG TGTCTTTGTC GGAATTATCG GAAATCACTT TGTTCCCTTC GTCTGTATCG 
CCAACATGGA AGAAAAGCTT TGGCAATGCT AATACGAATC TAAAGTATGG TTTGGAAGAC 
ATGTCGCTCA GCCATCGTCG TGGTCATAAA CGCACATACG ATGAACATTT GAACAATGTA 
CAACAGGGCG TCAACCATGA CATGAATCGT GTTCATGGTA GTGTTGGAGG TATGAGTGGT 
ATTGTTGGTA TTGGTATTGG TATTGGTGAT GGTGATGGTG ATGGTGATGT TGATTCTCGT 
ACCATTCATT TCGCCGAGTA TAAAAGTCGT GTGCAAGCCG TCAAAAAACA ATGGGTAGAC 
TCAACTGACA TTTCAGCACA ACTCGAGCGA TTCTTTCGAG TGTATAAAGA TGAATGTCTA 
AGTTGCACTC TTGGTAACCC AGACACAGAA ATTCGTGCTC ATACCGGGAA AGCATGCCCC 
GTACGCTTAT CTACTTGTTA TAAATGTGGC AAGGCCGACC ACAACTTGCG AGAATGTAAA 
CTACGTATTC GATTTCAAGG ACTTTGTTTA TTTTGTGGTT TGACAAAGTT TGAACATGCC 
GACAGTGATA TGGCTTACAC TTCTGACTGT AGGTCATGGG CTAGAAAGGC AAACTTGATA 
TCGTTAGTTT ACTATGCTTG GAACAATGTA CAATACCGAA GGACTATTGC AGATAAATTC 
TTACAAGGTG ATGTACGTGA TCAAGCTTTT TATGGATTTG TGTGTTGCTC TACAACTACG 
AATTCTGCAT TTGTTCTAGT TATTCATTAC TTGTTGTCTG ATGTTTTGCA AATCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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tlh2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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