cid1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

Data show that a hinge region (N164-N165) of terminal uridylyltransferase Cid1 is essential for catalytic activity.
Title: Structural plasticity of Cid1 provides a basis for its distributive RNA terminal uridylyl transferase activity.

Structural analysis of Cid1 bound to a non-hydrolyzable nucleotide UMPNPP and bound to its minimal pseudo-product ApU revealed new key residues for the substrate/product recognition.
Title: A critical switch in the enzymatic properties of the Cid1 protein deciphered from its product-bound crystal structure.

This work demonstrates that Cid1 is able to accommodate all 4 tested ribonucleotide triphosphates (NTP) in the active site, but there are differences in recognition that suggest UTP is the preferred NTP.
Title: Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity.

describe crystal structures of cytoplasmic terminal uridylyl transferase Cid1 in apo conformers and bound to UTP, and demonstrate that a single histidine residue, conserved in mammalian Cid1 orthologs, is responsible for discrimination between UTP and ATP
Title: Structural basis for the activity of a cytoplasmic RNA terminal uridylyl transferase.

Cid1 RNA binding properties, a feature with critical implications for miRNAs, histone mRNAs, and, more generally, cellular RNA degradation
Title: Functional implications from the Cid1 poly(U) polymerase crystal structure.