rgf3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	rgf3
Entrez Gene ID	2539437
Full Name	RhoGEF Rgf3
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001023105.2	NP_588115.1	RhoGEF Rgf3

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rgf3	NP_588115.1
Magnaporthe oryzae (rice blast fungus)	MGG_12644	XP_003721164.1
Neurospora crassa	NCU02131	XP_964289.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Arrestin family protein Art1 regulates the protein levels and localization of the Rho-GEF Rgf3, which in turn modulates active Rho1 levels during fission yeast cytokinesis.
Title: Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis.

Rgf3 is involved in cytokinesis in fission yeast.
Title: Rho1-GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast.

Rgf3p acts as a positive activator of Rho1p, probably activating the Rho functions that coordinate cell-wall biosynthesis to maintain cell integrity during septation
Title: Schizosaccharomyces pombe Rgf3p is a specific Rho1 GEF that regulates cell wall beta-glucan biosynthesis through the GTPase Rho1p.

The following rgf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rgf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99697	NM_001023105.2 Latest version!	Schizosaccharomyces pombe RhoGEF Rgf3 (rgf3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rgf3 ( NM_001023105.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588115.1 Schizosaccharomyces pombe RhoGEF Rgf3 (rgf3), mRNA.

Price & Availability↑

CloneID	OSc99697
Clone ID Related Accession (Same CDS sequence)	NM_001023105.2
Accession Version	NM_001023105.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	RhoGEF Rgf3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023105.1.

ATGAAGCTCT CCAATGAACT TTTTCATCGG AGCTCGAAAG ACCATGGTGG AAAGAGCCGA 
ATTTGTTTAG ACTCTTCAGA GGACACTTAT CCTCCTCATT CCTCTTCTCC ACCATCTTTT 
CAGAAACGTC TATCGTTTTC CGACTTTTCT ACCACCAGAC TCTTTTCTCC TCCTTTTCTT 
TCTAAACGTT CGAATAATTC TCCCCATCGG TTTTCTTATT CGCCTCCTCA ACATCCAGCA 
TCCATTAACT CTCGTAGAGT CGCGTCTTAC ACGGTTCAGT CATCTCCATC AAGAACAACG 
TATCGACAAT TGCCGAATGA ACCTCAAAAT TCTGCAGCCT ATACTACTTA CTCTTCATTT 
CCTAATGCCT TATTTGATGA TTTCAGTCCT AACAACCCGC TTGACACGGA CCCCTTTTTA 
ACATCTCCTG GAAACAAACA AAATACTGTA GATTCGTTTC GTCCATTACC TGAAACGCCT 
GTTTCTCCTG GTGGGTCCTT AGTGCATCCT TTACCACGTC CACCCCTTCC TTCTTCTGTT 
TCTTCGCATT CTTCTCCTTA CAGCACCACA TCCTCCACTA GTCTTTACTC TCTATACAAT 
GACATATCGT TATCCTGCTC ACCAGAGCCA TATCTTCCTT TATCACCTAC TCGATCACCG 
GCTCGCACTC CTAGTCCGAT TCGTCTTTAC TCGAGCGACG CTCTTCGTCC ACAGTCGCCT 
TTAAGCCCTT CCGTCGAATA TCTAACACCC CCAAATCCTT ATAGTTTAAA ATCTGATATT 
TCCTCTACAA GACAGCTACC AAAGATACCT GTCCAAGACT ATGCATCTGG AAAAATTTCT 
TCTCCTTTGA TAACTCGAAC TCACAGACGT GCTCAATCGG AAACCCTTTT TAGCAGCTGT 
AGAGAACCTT GGTTGGTCGG TAAATTATAC AAATGGTGCA AAGAAGAAGT GTTTACTGCT 
CTTGGTGGCC TGGTCCACGA AGGTGTGTCT CGTCGAGAAG TAGCTCAGGT GATGGCTACG 
CTATTTACTA TTCACATTGC ATCTATGGAA TTTTTGATTG CTGAAATTAT CGCGAAAAAC 
ATCCTTGGTG ATTGGATTAA TTACGGTCTT GTGGAAGTCA TAAATCTTGA AAAGCTTCAA 
ATTGCTTTCA CATCTAATGA ACCTCCTTCT GGTAGTGGAG TACTTCCATT TTTGACCAAC 
GGCGGCTGTT ACTCTTACAT TTGTAGATCC AGATCATGTC CTTCCAAATA TCAATGTTAT 
AGCTGTAGAT GTGCTCGTAA CTCGTCGCTT GAATTCACTT CTCTGCCAGG ACAGAGTAGC 
GATACTTGGA GCATTTTCTG GAACATTTCT TCTCTAAATT CATTACCATC TTCTCTCAGT 
AAAAGAGAGA TTGCTAGGCA AAATAATATC CACGAGCTTA TTTGTAAGGA ATCTGATTAC 
GTTGCAGACT TAAATACCCT AGCTGAACTG TTTAGGGACG GAATTGTGCA GCAGCAAGAT 
GCGATTGTTC CCAGCAATCG AGTGGCTGAC TTTATTCAAT CTGTATTTGG AAATGTTGAA 
TCAATCAGAC AATTGCATTC TCGACTGTTC TTACCACAAT TAATTATGAG GGAACGTCTC 
CAGGGCCCAG TAGTTTCAAT TATTGGTGAT ATCTTACTAG AGTGGATTCA TGCTGCTAAA 
TCCTCTTATA TTAATTATGC CAAACAGTTC CCTTTAGCAG ATGAAACTTA TAAGCTAGAA 
TGTCAAAGGA ATACTTACTT TGCACGTTGG CTTGCTGCTT GTCGATCAGA TCCTAGATGT 
CGTCGGTTGG ATTTCCAGCA TTTCTTGCAA AGACCTACTC AACGTTTACA AAGATACACG 
CTTGAGTTGG ATACAATTTT AAAGCATACC GAGCAGTCCA GCTGGGATTT TCAATTAATA 
ACTCAAGCTG TCAAAGAACT GAGAGCAACT TGCGAGGAAT GTGATGCCGT TATAGCTACC 
GTACTTGAGG CCAATCGTAT TCGCGATCTA TCGTATCAAC TTCTGTTTAA AAATCATGAA 
AGTGTCAATC TAGAGCTAAG GGATCCTGAA AGAGAGTTCT TTTTTGAAGG AATAGTTCAA 
CGTAGAAGTG ATTCCCGCTT GGATTGGTTG GACATCCACT TGTTTTTGCT CGATAATTAC 
TTGATCATGG CCAAAGCAAG AAAAGACAAA CGAACAAATG CTTCAAGATA TGTTGTTTCA 
AAAAGGCCAA TACCATTAGA TTTGCTTGTT CTAAGCCCTA AAATGGACGA TTTTCAACTT 
AAATCAAACA CTAATAAATT TCTTGGCAGT TTAGCTGGCA ATTTACCACA GGAATCTCTT 
ACCACTAAGA GTAAAAGGAA GAGTAAGGTG AATCTTGAAC TAATGTTCGA TGCCACTGCT 
GAAAAGAATA ACGAAAACTC AATGAACTCT GCTGTATTTG AGAAGTCTCA ACTTTACCCC 
TTCACAATAA GGCACCTTGG TGCTTACACC GCTAGTTATA CACTCTACGT TGAGTCACTT 
CAGCTTCGTA AACTCTGGGT TGAAAAAATT AATGTCGCAA AAAAACGACA CTCTCAGAAG 
ATAAATATTA AGAATCCGTT TGCATTGAAA GTTGTATCTG ACGTTGCCTT TCAGTACCCT 
CCTTCCGACT TAGTTAATGG AAATGAACCA TTAAACAGCT TTAATGAAAT TACTTTGGTT 
GAAGGCTCTT CGATTGATCG CGCACTCAAT GAGGTAGCGT GGAAACACCC AATTGTAAGT 
GAGGAGCTTT TGCCAGAGCC TATTGCCTAT GGCGATATAA GTTGTATTGC CCAGTTTAAT 
GACTACGAAG GTCATGTCTC GGTTTTGATT GCTACATCTA CCGGTATCTT TTTAGGCGCA 
TTTGGTGATT CCAGTGACAT TCGCGATTGG AAAAAGATCT CTAGTCAAAG ACGCGTTACC 
CAGCTTGGAG TTGTTGAGGA ATTTGATATC CTATTAGAGC TACGTGACAA AACCCTTTAT 
GCTCATAAAT TAAGTCGTAT TATTGAAATG GGTCTGATCG AATCCAAAAT TGCAGTTGTC 
ATTGGAACTC CGCATGCCGT ATCATTCTTT AAAATAGGTA AATTGTCTGA GGGGGCTTCT 
GTTAAACGTG AGCGAACTTT GGTTTTTTAT AAAGAGGGAC TCGGTAATAC TACAACCATA 
ATTTGCTGTG AACCAGTGAT CGGCTTAGGA CATAACTATC AGAAAACATA TGCCTTCAAG 
AGAAAAGATG TTACTTCCTT CAGAACTCTG GATGATTTTC ACGTAACGGC TAACTGTCAT 
TCAATTGATT GCTTTAAGTA TTCAATTGCT CTTTGTCACA ATAAAGGCAT TGACGTTCTG 
CGTTTGGACC CTAAATTAGC AGTTGGTTTT CCAAGCCCTT CAGTGCTTAA TGATACGTTG 
TTTCGGAACC GAATCAACAA CAGTAAGCCG CTTGGCGTCT TTCGCATCCA CGATCCTAGC 
TTATTTGCAT GCTGCTATCA ATTCGGTGCC GTGTTTGTCA ATGGAGAGGG TAGTATGGTT 
AATAAAGAGT GTTGGTTTGA TTGGATTGGC AAACCGAACT CTGTTACCAG CTGTCATGGA 
TATCTCATTG CATTTAATGA TGAATTTGTC GAAATATGGA ACACGCGAAC TAGGAAACTT 
AATCAAATTA TCCAAGGAAA TGACATTAAA TACTACCCTA GTAACAGTGA TTGGCTAGCC 
AATGGGAAAT ATATAATGTT TGGCATGGTT CATCCTCAGT ATCACGATCG CCATTTGATA 
CTCGCTTTAA ATAAGGCAAA AACAAACTCC TTCATAATTG AAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_588115.1
CDS	894..4721
Translation	MKLSNELFHRSSKDHGGKSRICLDSSEDTYPPHSSSPPSFQKRLSFSDFSTTRLFSPPFLSKRSNNSPHRFSYSPPQHPASINSRRVASYTVQSSPSRTTYRQLPNEPQNSAAYTTYSSFPNALFDDFSPNNPLDTDPFLTSPGNKQNTVDSFRPLPETPVSPGGSLVHPLPRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYSSDALRPQSPLSPSVEYLTPPNPYSLKSDISSTRQLPKIPVQDYASGKISSPLITRTHRRAQSETLFSSCREPWLVGKLYKWCKEEVFTALGGLVHEGVSRREVAQVMATLFTIHIASMEFLIAEIIAKNILGDWINYGLVEVINLEKLQIAFTSNEPPSGSGVLPFLTNGGCYSYICRSRSCPSKYQCYSCRCARNSSLEFTSLPGQSSDTWSIFWNISSLNSLPSSLSKREIARQNNIHELICKESDYVADLNTLAELFRDGIVQQQDAIVPSNRVADFIQSVFGNVESIRQLHSRLFLPQLIMRERLQGPVVSIIGDILLEWIHAAKSSYINYAKQFPLADETYKLECQRNTYFARWLAACRSDPRCRRLDFQHFLQRPTQRLQRYTLELDTILKHTEQSSWDFQLITQAVKELRATCEECDAVIATVLEANRIRDLSYQLLFKNHESVNLELRDPEREFFFEGIVQRRSDSRLDWLDIHLFLLDNYLIMAKARKDKRTNASRYVVSKRPIPLDLLVLSPKMDDFQLKSNTNKFLGSLAGNLPQESLTTKSKRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLYPFTIRHLGAYTASYTLYVESLQLRKLWVEKINVAKKRHSQKINIKNPFALKVVSDVAFQYPPSDLVNGNEPLNSFNEITLVEGSSIDRALNEVAWKHPIVSEELLPEPIAYGDISCIAQFNDYEGHVSVLIATSTGIFLGAFGDSSDIRDWKKISSQRRVTQLGVVEEFDILLELRDKTLYAHKLSRIIEMGLIESKIAVVIGTPHAVSFFKIGKLSEGASVKRERTLVFYKEGLGNTTTIICCEPVIGLGHNYQKTYAFKRKDVTSFRTLDDFHVTANCHSIDCFKYSIALCHNKGIDVLRLDPKLAVGFPSPSVLNDTLFRNRINNSKPLGVFRIHDPSLFACCYQFGAVFVNGEGSMVNKECWFDWIGKPNSVTSCHGYLIAFNDEFVEIWNTRTRKLNQIIQGNDIKYYPSNSDWLANGKYIMFGMVHPQYHDRHLILALNKAKTNSFIIED

Target ORF information:

RefSeq Version	NM_001023105.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe RhoGEF Rgf3 (rgf3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001023105.2

ORF Insert Sequence:

ATGAAGCTCT CCAATGAACT TTTTCATCGG AGCTCGAAAG ACCATGGTGG AAAGAGCCGA 
ATTTGTTTAG ACTCTTCAGA GGACACTTAT CCTCCTCATT CCTCTTCTCC ACCATCTTTT 
CAGAAACGTC TATCGTTTTC CGACTTTTCT ACCACCAGAC TCTTTTCTCC TCCTTTTCTT 
TCTAAACGTT CGAATAATTC TCCCCATCGG TTTTCTTATT CGCCTCCTCA ACATCCAGCA 
TCCATTAACT CTCGTAGAGT CGCGTCTTAC ACGGTTCAGT CATCTCCATC AAGAACAACG 
TATCGACAAT TGCCGAATGA ACCTCAAAAT TCTGCAGCCT ATACTACTTA CTCTTCATTT 
CCTAATGCCT TATTTGATGA TTTCAGTCCT AACAACCCGC TTGACACGGA CCCCTTTTTA 
ACATCTCCTG GAAACAAACA AAATACTGTA GATTCGTTTC GTCCATTACC TGAAACGCCT 
GTTTCTCCTG GTGGGTCCTT AGTGCATCCT TTACCACGTC CACCCCTTCC TTCTTCTGTT 
TCTTCGCATT CTTCTCCTTA CAGCACCACA TCCTCCACTA GTCTTTACTC TCTATACAAT 
GACATATCGT TATCCTGCTC ACCAGAGCCA TATCTTCCTT TATCACCTAC TCGATCACCG 
GCTCGCACTC CTAGTCCGAT TCGTCTTTAC TCGAGCGACG CTCTTCGTCC ACAGTCGCCT 
TTAAGCCCTT CCGTCGAATA TCTAACACCC CCAAATCCTT ATAGTTTAAA ATCTGATATT 
TCCTCTACAA GACAGCTACC AAAGATACCT GTCCAAGACT ATGCATCTGG AAAAATTTCT 
TCTCCTTTGA TAACTCGAAC TCACAGACGT GCTCAATCGG AAACCCTTTT TAGCAGCTGT 
AGAGAACCTT GGTTGGTCGG TAAATTATAC AAATGGTGCA AAGAAGAAGT GTTTACTGCT 
CTTGGTGGCC TGGTCCACGA AGGTGTGTCT CGTCGAGAAG TAGCTCAGGT GATGGCTACG 
CTATTTACTA TTCACATTGC ATCTATGGAA TTTTTGATTG CTGAAATTAT CGCGAAAAAC 
ATCCTTGGTG ATTGGATTAA TTACGGTCTT GTGGAAGTCA TAAATCTTGA AAAGCTTCAA 
ATTGCTTTCA CATCTAATGA ACCTCCTTCT GGTAGTGGAG TACTTCCATT TTTGACCAAC 
GGCGGCTGTT ACTCTTACAT TTGTAGATCC AGATCATGTC CTTCCAAATA TCAATGTTAT 
AGCTGTAGAT GTGCTCGTAA CTCGTCGCTT GAATTCACTT CTCTGCCAGG ACAGAGTAGC 
GATACTTGGA GCATTTTCTG GAACATTTCT TCTCTAAATT CATTACCATC TTCTCTCAGT 
AAAAGAGAGA TTGCTAGGCA AAATAATATC CACGAGCTTA TTTGTAAGGA ATCTGATTAC 
GTTGCAGACT TAAATACCCT AGCTGAACTG TTTAGGGACG GAATTGTGCA GCAGCAAGAT 
GCGATTGTTC CCAGCAATCG AGTGGCTGAC TTTATTCAAT CTGTATTTGG AAATGTTGAA 
TCAATCAGAC AATTGCATTC TCGACTGTTC TTACCACAAT TAATTATGAG GGAACGTCTC 
CAGGGCCCAG TAGTTTCAAT TATTGGTGAT ATCTTACTAG AGTGGATTCA TGCTGCTAAA 
TCCTCTTATA TTAATTATGC CAAACAGTTC CCTTTAGCAG ATGAAACTTA TAAGCTAGAA 
TGTCAAAGGA ATACTTACTT TGCACGTTGG CTTGCTGCTT GTCGATCAGA TCCTAGATGT 
CGTCGGTTGG ATTTCCAGCA TTTCTTGCAA AGACCTACTC AACGTTTACA AAGATACACG 
CTTGAGTTGG ATACAATTTT AAAGCATACC GAGCAGTCCA GCTGGGATTT TCAATTAATA 
ACTCAAGCTG TCAAAGAACT GAGAGCAACT TGCGAGGAAT GTGATGCCGT TATAGCTACC 
GTACTTGAGG CCAATCGTAT TCGCGATCTA TCGTATCAAC TTCTGTTTAA AAATCATGAA 
AGTGTCAATC TAGAGCTAAG GGATCCTGAA AGAGAGTTCT TTTTTGAAGG AATAGTTCAA 
CGTAGAAGTG ATTCCCGCTT GGATTGGTTG GACATCCACT TGTTTTTGCT CGATAATTAC 
TTGATCATGG CCAAAGCAAG AAAAGACAAA CGAACAAATG CTTCAAGATA TGTTGTTTCA 
AAAAGGCCAA TACCATTAGA TTTGCTTGTT CTAAGCCCTA AAATGGACGA TTTTCAACTT 
AAATCAAACA CTAATAAATT TCTTGGCAGT TTAGCTGGCA ATTTACCACA GGAATCTCTT 
ACCACTAAGA GTAAAAGGAA GAGTAAGGTG AATCTTGAAC TAATGTTCGA TGCCACTGCT 
GAAAAGAATA ACGAAAACTC AATGAACTCT GCTGTATTTG AGAAGTCTCA ACTTTACCCC 
TTCACAATAA GGCACCTTGG TGCTTACACC GCTAGTTATA CACTCTACGT TGAGTCACTT 
CAGCTTCGTA AACTCTGGGT TGAAAAAATT AATGTCGCAA AAAAACGACA CTCTCAGAAG 
ATAAATATTA AGAATCCGTT TGCATTGAAA GTTGTATCTG ACGTTGCCTT TCAGTACCCT 
CCTTCCGACT TAGTTAATGG AAATGAACCA TTAAACAGCT TTAATGAAAT TACTTTGGTT 
GAAGGCTCTT CGATTGATCG CGCACTCAAT GAGGTAGCGT GGAAACACCC AATTGTAAGT 
GAGGAGCTTT TGCCAGAGCC TATTGCCTAT GGCGATATAA GTTGTATTGC CCAGTTTAAT 
GACTACGAAG GTCATGTCTC GGTTTTGATT GCTACATCTA CCGGTATCTT TTTAGGCGCA 
TTTGGTGATT CCAGTGACAT TCGCGATTGG AAAAAGATCT CTAGTCAAAG ACGCGTTACC 
CAGCTTGGAG TTGTTGAGGA ATTTGATATC CTATTAGAGC TACGTGACAA AACCCTTTAT 
GCTCATAAAT TAAGTCGTAT TATTGAAATG GGTCTGATCG AATCCAAAAT TGCAGTTGTC 
ATTGGAACTC CGCATGCCGT ATCATTCTTT AAAATAGGTA AATTGTCTGA GGGGGCTTCT 
GTTAAACGTG AGCGAACTTT GGTTTTTTAT AAAGAGGGAC TCGGTAATAC TACAACCATA 
ATTTGCTGTG AACCAGTGAT CGGCTTAGGA CATAACTATC AGAAAACATA TGCCTTCAAG 
AGAAAAGATG TTACTTCCTT CAGAACTCTG GATGATTTTC ACGTAACGGC TAACTGTCAT 
TCAATTGATT GCTTTAAGTA TTCAATTGCT CTTTGTCACA ATAAAGGCAT TGACGTTCTG 
CGTTTGGACC CTAAATTAGC AGTTGGTTTT CCAAGCCCTT CAGTGCTTAA TGATACGTTG 
TTTCGGAACC GAATCAACAA CAGTAAGCCG CTTGGCGTCT TTCGCATCCA CGATCCTAGC 
TTATTTGCAT GCTGCTATCA ATTCGGTGCC GTGTTTGTCA ATGGAGAGGG TAGTATGGTT 
AATAAAGAGT GTTGGTTTGA TTGGATTGGC AAACCGAACT CTGTTACCAG CTGTCATGGA 
TATCTCATTG CATTTAATGA TGAATTTGTC GAAATATGGA ACACGCGAAC TAGGAAACTT 
AATCAAATTA TCCAAGGAAA TGACATTAAA TACTACCCTA GTAACAGTGA TTGGCTAGCC 
AATGGGAAAT ATATAATGTT TGGCATGGTT CATCCTCAGT ATCACGATCG CCATTTGATA 
CTCGCTTTAA ATAAGGCAAA AACAAACTCC TTCATAATTG AAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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rgf3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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