rgf3 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	rgf3
Entrez Gene ID	3880429
Full Name	Rgf3-like
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011394641.1	XP_011392943.1	rho guanyl nucleotide exchange factor
XM_011394640.1	XP_011392942.1	rho guanyl nucleotide exchange factor, variant

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rgf3	NP_588115.1
Magnaporthe oryzae (rice blast fungus)	MGG_12644	XP_003721164.1
Neurospora crassa	NCU02131	XP_964289.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rgf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rgf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12757	XM_011394640.1 Latest version!	Neurospora crassa OR74A rho guanyl nucleotide exchange factor, variant (rgf3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ONf12758	XM_011394641.1 Latest version!	Neurospora crassa OR74A rho guanyl nucleotide exchange factor (rgf3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rgf3 ( XM_011394640.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011392942.1 Neurospora crassa OR74A rho guanyl nucleotide exchange factor, variant (rgf3), mRNA.

Price & Availability↑

CloneID	ONf12757
Clone ID Related Accession (Same CDS sequence)	XM_011394640.1
Accession Version	XM_011394640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	rho guanyl nucleotide exchange factor, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCGTTTC GCGGCGACGA TTCACGCCGC TATGGCCATG TGCCGCCTGC TCAATACCCC 
GTCGCCGGCG CCGCGCAGGA CAACAGCAAC TCGCCGTATC CTCCCCAGCG ACGCATGAGC 
TTCAACACGG GCGACGATGC CGCCATGTTC GACCCGAGCG CTGCCCGCCA GCAGCCCGCC 
ATTTACACTG GAGTCCCCGT TGTCGGCAGG GCCGAGGAGG AGCTCTTCCT CGGCAGCCCC 
ACGTCACAGG ATGCGCCGGC CGCCCAAAAT CGCCTCAGCT ACAAATCTCA GCACATCGCC 
ATGGCGGGCT ATCAACACCA GTATCAGGCC CAAACAAGCG CGCCGCCGCC CGCACCGGCG 
CACTCGACAT ATAACCCCCA GGCCTTTGCT CGCACTCAAT CGACCTCTCT CCCGTACCAC 
CCCGCCCCGG CCTCGAGATA CGGCGCTCCG ACGAGCCCGA CCTACGCGCC CACCCCTACA 
ACCTACACTC CGCCAGCTTA TAACCCGGCG GCGTACGCCA ACACAAATAC GGCGACGCCC 
CAGAGACATT CTTCGGTCGC TTCAGTGGCT TCCTCGGGAT ACAATGGATA CACGTATGGC 
TATACTTCTC CCGGCATCCC GCAGTCTGGG TTTGGGCAAT CTCCCCAGTC GGCTACCGCT 
CCATCCATAT CCAGCTATGA GCCTGCCCGT AGTCCACAGT ATCCTCCGTC CTCTGCCACC 
GCCACATCAC CGCAATATGA CCAATCCTAC CCCCCGGCGA ATGCGTACAA CACCCAGCAG 
TACTACGGCT CCTATCCCGT GAATGCCTCG GGCATGGCGT ATAACACCAT GACATCTCAG 
GCGCCCTATC CCACTGCACA TGCTCAGATG CCCGCCGGAC CTAGTTACGC TTCTGCGGAT 
CACGCCGCGT TTTCCGGTCG GAACTCTAGA TCAGATTCCC AGGTCTCTGC GCCATCTCCT 
CCGCCGCATT CACAAGCACC CGGGGGGCTT ACAAGACACC CGACAAATGC TCCGCTGCCT 
AGCCGTCCCA TGGATGACTT GCCAGAGCAC CATAGCTGGG GGCCCAACGG GCAAAGTAAT 
GAGCTGCAAA CCCAAGACGC CTTGTTCGGT GATATCATCT CGGAGGTGGA GCCACGACAG 
TACCGTGGCG TTAATGGCAA CATATCAGAG GACGACCAGG GACTGCACCG CTACAGTTCG 
ACTGCTTCGA CAAATGTGGG ATCCGCTGCA GCGGTACATC GCTATCCTTC TAATGCATCG 
ACAGTGAACA GAAATGATAT CCCCGATACC TATCCCAATA CTTACGACTA CGATGACGAC 
GAGAGCGACC CCGAAGGTGC TGCTGGCCTT CTAGCCATGC AGGAGGACAT GGACGACCGA 
CGTTTTGGCT TTGGTGGGAT ATCGTTCCCG ACTTATATGG AGCCTCCGGC AGCGCAACAG 
CAGACCTCGC CGACACACCC GATACAGCCG CCGGGACACC AACGAGAGTT ACCACCACCG 
CCTGAGGAAC AAGGTACGGA AAGTGATTTT GGCGGAATGG ATCTCGGCTT GTATGGGGGT 
GGCTATGCGG GAAACCTGCA CTACGGGAAT GAAGTTGGCT CCCCACCCGC AACTTCTGCC 
CACTTTGACC AAGGGCCCAG GCCGTTGCCA TATCCGCAAA ACACGGGAAG CGAGTATGCT 
CCGTTCTCCC AAGCTGCGGT TGACTATGGT GGAACCGGTG GCCTTCAGGC CCCGCAAACA 
CACCGGCTGA GCTTCGATGA GGGAGATGAG CATGTCTCGA TTCACTCCAG GCACAGCGGA 
AGTGACTCGC CTACGAAGGA AGAGTATCCT GATATGTTCT ATCATCCTGG GATATCTAAT 
CGGCCTCTCC CGGCTGTTCC TCCTCTTGGC GAAAGCCGTC CAGTACTGTC GGTAGTGCCC 
CCTAGTAGGG TCGCTTCTCA ACAGGGGTAC AATATGGATG TAAATCAGCG CCTCATGTAT 
GACCAACAAG GTCAAGCTGC AGTGGAGAGA TCGATATCAC TTTCCAGCCA CAGCAATACC 
CCTCCTGTCC ATACACCGGC ACGATCCAGA ACCGATGCCG CTGAGGAGCG GAGGAGAGCA 
TTGAAGCATG CTAACCAGCA TCATTCTCAT CTGAGCCACC ATAGTGTGTC TCAGGATGGC 
TACGACTCTG GGACACCAAC GTCGCTTGCT GCCTATGATA TGATCACCTT GCCTGGCAGG 
AAGAGGAAGT TTATTCCATC GAAGCTCACG GCTACGGAGA TTAAGCGTTG CGCCGAACCG 
TGGGCGCTCA GTAGCGTTAC TGCCTGGATT CGTGAGATGG CAGACGGCGA AGTGGACCTC 
AAGAGAAAAT CCATCGAAGA GGGAATTGTG AAGCTTTTCT GTCACAAGGT TCCTACCATG 
AACGTTGCTG ACGCGGAAAG TTTGAGTGCT ACAGTAGTGG ACTCGATGTT TGCGTCCGGT 
CTCTTGATTC CAGATGAGGA ATGGGTCAGG TTTGGGACCG GCCACATGAC AGGTGTCTTG 
TGGCAACTCA CTGGCCATGG TTGCTACGCG CCCAAGTTGC ATGAGGCCGA GGAGTCTGCT 
CCTAAACTTA ACGATAACGG CATTCCAGTA AGGTGCTACT CGCATCACTG CGGTAGAACG 
CTGAAGAAGG CCAACCTGGA TCACCTGCTT TCTGATGATC CCGTGGTTCA GGACTGGGCG 
ACCTTCCATG GAGTCAAGCC GGAGGATTGC GAAAAGAAGA GCAAAAAGGA GATTGAGAGA 
CAAAATGTTC TGCACGAGAT TGTCACCAGT GAAGAGGAGT ACATGGCCCA GCTCGACGTT 
GTTCGGCTCC TCTACAGAGA TCAACTCCGC GTGCACCAGC CCCCTGTTCT TCCCAACAGC 
AGGAGGGACA AGTTTCTGGA AGCAGTCTTT GGTAAAGTCG ATACGGTGCA GCAAATCAAC 
AAGGACCACT TGCTTGCCCA GCTGAAGTAT CGCCAACAGG AACAGGCGCC CTTCATTGTT 
GGGTTTAGTG ACTTGTTCAG AGAGTGGATT CGCAAGGCCA GGCCCATTTA CATCGAATAC 
TGCTCGTCTT ATCCGAATTC GGAGTACCTT ATCCGAAAGG AAAGTAATAG GAATATTCTC 
TTCCGCGAGT TTCTGGGACA CGTACAAAAC CATAGACGGT CCAAGCGTTT GGGATGGACT 
ACGTTTGTGA AGGCGCCCAT CACACGTCTG CAAAGATATT CTTTGTTGCT CAGCACGGTT 
TTGAAGAACA CGATTGAAGA CAGTGAAGAG AAGCAGAACC TCATCAAGGC AATCGACGAG 
ATCAGGTCAG TCACAAAGGA GTGCGATGAC AGAGTTGCTG AGATGACCAA GAAGGTGTCC 
TTGCTTGAGC TACAACAGCA GCTAGTCCTT CGACCCGGTT TCCAATCAGT CCTCAATCTT 
GATCACCTTG GGAGAGAGCT TCTCAAGCAA GGCGAGCTTC AGCGACAGGG TTCCAAGGGA 
GTACGCTGGG TTGACACACA GGCCCTGCTG TTTGATCATT ATTTCATCTT GGCCAAAGCA 
GTCTCAACCA AGGACGGGAG GAATGAGAAG AAATACGACG TGTCGAAAGA GCCTATACCC 
ATGCCACTCC TCTTCCTGGA AAGCATGCAC GACGAACCTG TTACGAAACA AAAGGGTCTC 
GCAGCACCTC TGACCCGTAC AGCAGCTACC GCTGGATCCG GGACACAGCT TAACAAGATT 
GCCTCTAACG GAGGCGATCG GCCCGGCCTG GAGCATTCAG CAACAAGTGT CGACGATGGA 
AAGATCATCT ACCCGTTTAG GATAAAACAC CTCGGCCACG AGATTTACAC GCTCTACGCT 
TCCTCTGCCC TGGACCGCTC GGAATGGTGT AAGGCGATTG TGGACGCGAA GACAAGACAC 
GCCGAAGCGC TACATGCACA GAACGCCGAG CCTTTCCGCT TGCGAGTTCT TTCTGACGGT 
GCTTTTGCGT ACGATTCTTA TTCAACCTTT GGTAGGCAGC CGGGCGTGCC GATTAGTGGA 
ACACCGTTGG ACAGGGCGAT ACGAGGAATG GAGGAGGTAT ACGGTAAAGG TCGCGGTCCC 
CCGCCGGTAT GCAGAGCTCA GGTAAACTGT GCTACCGCTT TCAAATCGTT CGGCAAGTCC 
ATCATCGCCA TTGGCACGGA TTATGGCGTC TACATCTCGG AAGCATCCAA CCCCCGAGGC 
TGGACACGCT CTGTCCAGAT CAGCAAGGTC ACCCAGATCG CAGTCCTCGA GGACTTCTCT 
GTCTGCATTC TCATCTCCGA CAGGTCACTC ATCTCCTACC CCCTCGACGT GATCGCGCCC 
GTCTCCAACT TTCCGTCCCC CGTTCATGAT AACCCCAGAC GGGCTCCCCA GCGTCTTGCC 
AAAGATGTCA CCTTCTTTGC CACCGCCCGC ATGAAGGACC GCACCCTTCT CTTCTACAAA 
CGCAAGGAAG GCATGCACAA CACCTTCAAG GTCCTCGAGC CCGTCTTCCA AAAGGCAACC 
GAAAAGAAAT CCCGTATCTT CGGCAGCCGC AAATTCGGCT CCGGCGCGAC CGAGTCCTTC 
CGCGACTACG ACGAATTTTA CATACCCGCC GAGTGCTACT CGCTCAACTT GTTCCAAAGC 
TACATTGCCG TGGCCTCCGC CAAGGGCTTT GAGCTACTGA CCCTCGACAA AAAAGTAACC 
CAGTCCATCC CGCGCGACCT CAACCTCCCC GCCATCGCCA ACATCGCCTC CCGCATCAAG 
GACCAGCGGC CCTTGGGCAT GTTCAAGCTC AACGACCAGG AATTCCTTCT CACCTACGAG 
GACTGCGCCG TCTACGTCGA CAAGCACGGC GAGATCTCCC GCACTTTGAT CATGGAGTAC 
TCGGGCAAAC AGAAAAAGGC CAAAGCAGCC ACCATGTTTG GCCAGTACCT CCTCTTGTTC 
AACGAGGATT ACGTCGAAGT CCGCAACGCC GAGAATGGGC GCTTGCGGCA GATCATTGCC 
GGCCGCGATG TAAGGTGTTT GGACTATGGG TTTAGAGGCC CCACGGGCAG TGGCCAGCAG 
CATGCGCAGC CGCATACTTT GTTGCAGGCG GGAGTGCTGC AGGATACTAA GGGGACGGTC 
AAGATTTGCA TGAGTCATCC TGAGGTGCCC GGTGGACAGA TTGTGTTGGA GATGTTGCTT 
AATGATGGGC ATGCGGAGAA GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011392942.1
CDS	603..5789
Translation	MSFRGDDSRRYGHVPPAQYPVAGAAQDNSNSPYPPQRRMSFNTGDDAAMFDPSAARQQPAIYTGVPVVGRAEEELFLGSPTSQDAPAAQNRLSYKSQHIAMAGYQHQYQAQTSAPPPAPAHSTYNPQAFARTQSTSLPYHPAPASRYGAPTSPTYAPTPTTYTPPAYNPAAYANTNTATPQRHSSVASVASSGYNGYTYGYTSPGIPQSGFGQSPQSATAPSISSYEPARSPQYPPSSATATSPQYDQSYPPANAYNTQQYYGSYPVNASGMAYNTMTSQAPYPTAHAQMPAGPSYASADHAAFSGRNSRSDSQVSAPSPPPHSQAPGGLTRHPTNAPLPSRPMDDLPEHHSWGPNGQSNELQTQDALFGDIISEVEPRQYRGVNGNISEDDQGLHRYSSTASTNVGSAAAVHRYPSNASTVNRNDIPDTYPNTYDYDDDESDPEGAAGLLAMQEDMDDRRFGFGGISFPTYMEPPAAQQQTSPTHPIQPPGHQRELPPPPEEQGTESDFGGMDLGLYGGGYAGNLHYGNEVGSPPATSAHFDQGPRPLPYPQNTGSEYAPFSQAAVDYGGTGGLQAPQTHRLSFDEGDEHVSIHSRHSGSDSPTKEEYPDMFYHPGISNRPLPAVPPLGESRPVLSVVPPSRVASQQGYNMDVNQRLMYDQQGQAAVERSISLSSHSNTPPVHTPARSRTDAAEERRRALKHANQHHSHLSHHSVSQDGYDSGTPTSLAAYDMITLPGRKRKFIPSKLTATEIKRCAEPWALSSVTAWIREMADGEVDLKRKSIEEGIVKLFCHKVPTMNVADAESLSATVVDSMFASGLLIPDEEWVRFGTGHMTGVLWQLTGHGCYAPKLHEAEESAPKLNDNGIPVRCYSHHCGRTLKKANLDHLLSDDPVVQDWATFHGVKPEDCEKKSKKEIERQNVLHEIVTSEEEYMAQLDVVRLLYRDQLRVHQPPVLPNSRRDKFLEAVFGKVDTVQQINKDHLLAQLKYRQQEQAPFIVGFSDLFREWIRKARPIYIEYCSSYPNSEYLIRKESNRNILFREFLGHVQNHRRSKRLGWTTFVKAPITRLQRYSLLLSTVLKNTIEDSEEKQNLIKAIDEIRSVTKECDDRVAEMTKKVSLLELQQQLVLRPGFQSVLNLDHLGRELLKQGELQRQGSKGVRWVDTQALLFDHYFILAKAVSTKDGRNEKKYDVSKEPIPMPLLFLESMHDEPVTKQKGLAAPLTRTAATAGSGTQLNKIASNGGDRPGLEHSATSVDDGKIIYPFRIKHLGHEIYTLYASSALDRSEWCKAIVDAKTRHAEALHAQNAEPFRLRVLSDGAFAYDSYSTFGRQPGVPISGTPLDRAIRGMEEVYGKGRGPPPVCRAQVNCATAFKSFGKSIIAIGTDYGVYISEASNPRGWTRSVQISKVTQIAVLEDFSVCILISDRSLISYPLDVIAPVSNFPSPVHDNPRRAPQRLAKDVTFFATARMKDRTLLFYKRKEGMHNTFKVLEPVFQKATEKKSRIFGSRKFGSGATESFRDYDEFYIPAECYSLNLFQSYIAVASAKGFELLTLDKKVTQSIPRDLNLPAIANIASRIKDQRPLGMFKLNDQEFLLTYEDCAVYVDKHGEISRTLIMEYSGKQKKAKAATMFGQYLLLFNEDYVEVRNAENGRLRQIIAGRDVRCLDYGFRGPTGSGQQHAQPHTLLQAGVLQDTKGTVKICMSHPEVPGGQIVLEMLLNDGHAEKA

Target ORF information:

RefSeq Version	XM_011394640.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A rho guanyl nucleotide exchange factor, variant (rgf3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394640.1

ORF Insert Sequence:

ATGTCGTTTC GCGGCGACGA TTCACGCCGC TATGGCCATG TGCCGCCTGC TCAATACCCC 
GTCGCCGGCG CCGCGCAGGA CAACAGCAAC TCGCCGTATC CTCCCCAGCG ACGCATGAGC 
TTCAACACGG GCGACGATGC CGCCATGTTC GACCCGAGCG CTGCCCGCCA GCAGCCCGCC 
ATTTACACTG GAGTCCCCGT TGTCGGCAGG GCCGAGGAGG AGCTCTTCCT CGGCAGCCCC 
ACGTCACAGG ATGCGCCGGC CGCCCAAAAT CGCCTCAGCT ACAAATCTCA GCACATCGCC 
ATGGCGGGCT ATCAACACCA GTATCAGGCC CAAACAAGCG CGCCGCCGCC CGCACCGGCG 
CACTCGACAT ATAACCCCCA GGCCTTTGCT CGCACTCAAT CGACCTCTCT CCCGTACCAC 
CCCGCCCCGG CCTCGAGATA CGGCGCTCCG ACGAGCCCGA CCTACGCGCC CACCCCTACA 
ACCTACACTC CGCCAGCTTA TAACCCGGCG GCGTACGCCA ACACAAATAC GGCGACGCCC 
CAGAGACATT CTTCGGTCGC TTCAGTGGCT TCCTCGGGAT ACAATGGATA CACGTATGGC 
TATACTTCTC CCGGCATCCC GCAGTCTGGG TTTGGGCAAT CTCCCCAGTC GGCTACCGCT 
CCATCCATAT CCAGCTATGA GCCTGCCCGT AGTCCACAGT ATCCTCCGTC CTCTGCCACC 
GCCACATCAC CGCAATATGA CCAATCCTAC CCCCCGGCGA ATGCGTACAA CACCCAGCAG 
TACTACGGCT CCTATCCCGT GAATGCCTCG GGCATGGCGT ATAACACCAT GACATCTCAG 
GCGCCCTATC CCACTGCACA TGCTCAGATG CCCGCCGGAC CTAGTTACGC TTCTGCGGAT 
CACGCCGCGT TTTCCGGTCG GAACTCTAGA TCAGATTCCC AGGTCTCTGC GCCATCTCCT 
CCGCCGCATT CACAAGCACC CGGGGGGCTT ACAAGACACC CGACAAATGC TCCGCTGCCT 
AGCCGTCCCA TGGATGACTT GCCAGAGCAC CATAGCTGGG GGCCCAACGG GCAAAGTAAT 
GAGCTGCAAA CCCAAGACGC CTTGTTCGGT GATATCATCT CGGAGGTGGA GCCACGACAG 
TACCGTGGCG TTAATGGCAA CATATCAGAG GACGACCAGG GACTGCACCG CTACAGTTCG 
ACTGCTTCGA CAAATGTGGG ATCCGCTGCA GCGGTACATC GCTATCCTTC TAATGCATCG 
ACAGTGAACA GAAATGATAT CCCCGATACC TATCCCAATA CTTACGACTA CGATGACGAC 
GAGAGCGACC CCGAAGGTGC TGCTGGCCTT CTAGCCATGC AGGAGGACAT GGACGACCGA 
CGTTTTGGCT TTGGTGGGAT ATCGTTCCCG ACTTATATGG AGCCTCCGGC AGCGCAACAG 
CAGACCTCGC CGACACACCC GATACAGCCG CCGGGACACC AACGAGAGTT ACCACCACCG 
CCTGAGGAAC AAGGTACGGA AAGTGATTTT GGCGGAATGG ATCTCGGCTT GTATGGGGGT 
GGCTATGCGG GAAACCTGCA CTACGGGAAT GAAGTTGGCT CCCCACCCGC AACTTCTGCC 
CACTTTGACC AAGGGCCCAG GCCGTTGCCA TATCCGCAAA ACACGGGAAG CGAGTATGCT 
CCGTTCTCCC AAGCTGCGGT TGACTATGGT GGAACCGGTG GCCTTCAGGC CCCGCAAACA 
CACCGGCTGA GCTTCGATGA GGGAGATGAG CATGTCTCGA TTCACTCCAG GCACAGCGGA 
AGTGACTCGC CTACGAAGGA AGAGTATCCT GATATGTTCT ATCATCCTGG GATATCTAAT 
CGGCCTCTCC CGGCTGTTCC TCCTCTTGGC GAAAGCCGTC CAGTACTGTC GGTAGTGCCC 
CCTAGTAGGG TCGCTTCTCA ACAGGGGTAC AATATGGATG TAAATCAGCG CCTCATGTAT 
GACCAACAAG GTCAAGCTGC AGTGGAGAGA TCGATATCAC TTTCCAGCCA CAGCAATACC 
CCTCCTGTCC ATACACCGGC ACGATCCAGA ACCGATGCCG CTGAGGAGCG GAGGAGAGCA 
TTGAAGCATG CTAACCAGCA TCATTCTCAT CTGAGCCACC ATAGTGTGTC TCAGGATGGC 
TACGACTCTG GGACACCAAC GTCGCTTGCT GCCTATGATA TGATCACCTT GCCTGGCAGG 
AAGAGGAAGT TTATTCCATC GAAGCTCACG GCTACGGAGA TTAAGCGTTG CGCCGAACCG 
TGGGCGCTCA GTAGCGTTAC TGCCTGGATT CGTGAGATGG CAGACGGCGA AGTGGACCTC 
AAGAGAAAAT CCATCGAAGA GGGAATTGTG AAGCTTTTCT GTCACAAGGT TCCTACCATG 
AACGTTGCTG ACGCGGAAAG TTTGAGTGCT ACAGTAGTGG ACTCGATGTT TGCGTCCGGT 
CTCTTGATTC CAGATGAGGA ATGGGTCAGG TTTGGGACCG GCCACATGAC AGGTGTCTTG 
TGGCAACTCA CTGGCCATGG TTGCTACGCG CCCAAGTTGC ATGAGGCCGA GGAGTCTGCT 
CCTAAACTTA ACGATAACGG CATTCCAGTA AGGTGCTACT CGCATCACTG CGGTAGAACG 
CTGAAGAAGG CCAACCTGGA TCACCTGCTT TCTGATGATC CCGTGGTTCA GGACTGGGCG 
ACCTTCCATG GAGTCAAGCC GGAGGATTGC GAAAAGAAGA GCAAAAAGGA GATTGAGAGA 
CAAAATGTTC TGCACGAGAT TGTCACCAGT GAAGAGGAGT ACATGGCCCA GCTCGACGTT 
GTTCGGCTCC TCTACAGAGA TCAACTCCGC GTGCACCAGC CCCCTGTTCT TCCCAACAGC 
AGGAGGGACA AGTTTCTGGA AGCAGTCTTT GGTAAAGTCG ATACGGTGCA GCAAATCAAC 
AAGGACCACT TGCTTGCCCA GCTGAAGTAT CGCCAACAGG AACAGGCGCC CTTCATTGTT 
GGGTTTAGTG ACTTGTTCAG AGAGTGGATT CGCAAGGCCA GGCCCATTTA CATCGAATAC 
TGCTCGTCTT ATCCGAATTC GGAGTACCTT ATCCGAAAGG AAAGTAATAG GAATATTCTC 
TTCCGCGAGT TTCTGGGACA CGTACAAAAC CATAGACGGT CCAAGCGTTT GGGATGGACT 
ACGTTTGTGA AGGCGCCCAT CACACGTCTG CAAAGATATT CTTTGTTGCT CAGCACGGTT 
TTGAAGAACA CGATTGAAGA CAGTGAAGAG AAGCAGAACC TCATCAAGGC AATCGACGAG 
ATCAGGTCAG TCACAAAGGA GTGCGATGAC AGAGTTGCTG AGATGACCAA GAAGGTGTCC 
TTGCTTGAGC TACAACAGCA GCTAGTCCTT CGACCCGGTT TCCAATCAGT CCTCAATCTT 
GATCACCTTG GGAGAGAGCT TCTCAAGCAA GGCGAGCTTC AGCGACAGGG TTCCAAGGGA 
GTACGCTGGG TTGACACACA GGCCCTGCTG TTTGATCATT ATTTCATCTT GGCCAAAGCA 
GTCTCAACCA AGGACGGGAG GAATGAGAAG AAATACGACG TGTCGAAAGA GCCTATACCC 
ATGCCACTCC TCTTCCTGGA AAGCATGCAC GACGAACCTG TTACGAAACA AAAGGGTCTC 
GCAGCACCTC TGACCCGTAC AGCAGCTACC GCTGGATCCG GGACACAGCT TAACAAGATT 
GCCTCTAACG GAGGCGATCG GCCCGGCCTG GAGCATTCAG CAACAAGTGT CGACGATGGA 
AAGATCATCT ACCCGTTTAG GATAAAACAC CTCGGCCACG AGATTTACAC GCTCTACGCT 
TCCTCTGCCC TGGACCGCTC GGAATGGTGT AAGGCGATTG TGGACGCGAA GACAAGACAC 
GCCGAAGCGC TACATGCACA GAACGCCGAG CCTTTCCGCT TGCGAGTTCT TTCTGACGGT 
GCTTTTGCGT ACGATTCTTA TTCAACCTTT GGTAGGCAGC CGGGCGTGCC GATTAGTGGA 
ACACCGTTGG ACAGGGCGAT ACGAGGAATG GAGGAGGTAT ACGGTAAAGG TCGCGGTCCC 
CCGCCGGTAT GCAGAGCTCA GGTAAACTGT GCTACCGCTT TCAAATCGTT CGGCAAGTCC 
ATCATCGCCA TTGGCACGGA TTATGGCGTC TACATCTCGG AAGCATCCAA CCCCCGAGGC 
TGGACACGCT CTGTCCAGAT CAGCAAGGTC ACCCAGATCG CAGTCCTCGA GGACTTCTCT 
GTCTGCATTC TCATCTCCGA CAGGTCACTC ATCTCCTACC CCCTCGACGT GATCGCGCCC 
GTCTCCAACT TTCCGTCCCC CGTTCATGAT AACCCCAGAC GGGCTCCCCA GCGTCTTGCC 
AAAGATGTCA CCTTCTTTGC CACCGCCCGC ATGAAGGACC GCACCCTTCT CTTCTACAAA 
CGCAAGGAAG GCATGCACAA CACCTTCAAG GTCCTCGAGC CCGTCTTCCA AAAGGCAACC 
GAAAAGAAAT CCCGTATCTT CGGCAGCCGC AAATTCGGCT CCGGCGCGAC CGAGTCCTTC 
CGCGACTACG ACGAATTTTA CATACCCGCC GAGTGCTACT CGCTCAACTT GTTCCAAAGC 
TACATTGCCG TGGCCTCCGC CAAGGGCTTT GAGCTACTGA CCCTCGACAA AAAAGTAACC 
CAGTCCATCC CGCGCGACCT CAACCTCCCC GCCATCGCCA ACATCGCCTC CCGCATCAAG 
GACCAGCGGC CCTTGGGCAT GTTCAAGCTC AACGACCAGG AATTCCTTCT CACCTACGAG 
GACTGCGCCG TCTACGTCGA CAAGCACGGC GAGATCTCCC GCACTTTGAT CATGGAGTAC 
TCGGGCAAAC AGAAAAAGGC CAAAGCAGCC ACCATGTTTG GCCAGTACCT CCTCTTGTTC 
AACGAGGATT ACGTCGAAGT CCGCAACGCC GAGAATGGGC GCTTGCGGCA GATCATTGCC 
GGCCGCGATG TAAGGTGTTT GGACTATGGG TTTAGAGGCC CCACGGGCAG TGGCCAGCAG 
CATGCGCAGC CGCATACTTT GTTGCAGGCG GGAGTGCTGC AGGATACTAA GGGGACGGTC 
AAGATTTGCA TGAGTCATCC TGAGGTGCCC GGTGGACAGA TTGTGTTGGA GATGTTGCTT 
AATGATGGGC ATGCGGAGAA GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgf3 ( XM_011394641.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011392943.1 Neurospora crassa OR74A rho guanyl nucleotide exchange factor (rgf3), mRNA.

Price & Availability↑

CloneID	ONf12758
Clone ID Related Accession (Same CDS sequence)	XM_011394641.1
Accession Version	XM_011394641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	rho guanyl nucleotide exchange factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCGTTTC GCGGCGACGA TTCACGCCGC TATGGCCATG TGCCGCCTGC TCAATACCCC 
GTCGCCGGCG CCGCGCAGGA CAACAGCAAC TCGCCGTATC CTCCCCAGCG ACGCATGAGC 
TTCAACACGG GCGACGATGC CGCCATGTTC GACCCGAGCG CTGCCCGCCA GCAGCCCGCC 
ATTTACACTG GAGTCCCCGT TGTCGGCAGG GCCGAGGAGG AGCTCTTCCT CGGCAGCCCC 
ACGTCACAGG ATGCGCCGGC CGCCCAAAAT CGCCTCAGCT ACAAATCTCA GCACATCGCC 
ATGGCGGGCT ATCAACACCA GTATCAGGCC CAAACAAGCG CGCCGCCGCC CGCACCGGCG 
CACTCGACAT ATAACCCCCA GGCCTTTGCT CGCACTCAAT CGACCTCTCT CCCGTACCAC 
CCCGCCCCGG CCTCGAGATA CGGCGCTCCG ACGAGCCCGA CCTACGCGCC CACCCCTACA 
ACCTACACTC CGCCAGCTTA TAACCCGGCG GCGTACGCCA ACACAAATAC GGCGACGCCC 
CAGAGACATT CTTCGGTCGC TTCAGTGGCT TCCTCGGGAT ACAATGGATA CACGTATGGC 
TATACTTCTC CCGGCATCCC GCAGTCTGGG TTTGGGCAAT CTCCCCAGTC GGCTACCGCT 
CCATCCATAT CCAGCTATGA GCCTGCCCGT AGTCCACAGT ATCCTCCGTC CTCTGCCACC 
GCCACATCAC CGCAATATGA CCAATCCTAC CCCCCGGCGA ATGCGTACAA CACCCAGCAG 
TACTACGGCT CCTATCCCGT GAATGCCTCG GGCATGGCGT ATAACACCAT GACATCTCAG 
GCGCCCTATC CCACTGCACA TGCTCAGATG CCCGCCGGAC CTAGTTACGC TTCTGCGGAT 
CACGCCGCGT TTTCCGGTCG GAACTCTAGA TCAGATTCCC AGGTCTCTGC GCCATCTCCT 
CCGCCGCATT CACAAGCACC CGGGGGGCTT ACAAGACACC CGACAAATGC TCCGCTGCCT 
AGCCGTCCCA TGGATGACTT GCCAGAGCAC CATAGCTGGG GGCCCAACGG GCAAAGTAAT 
GAGCTGCAAA CCCAAGACGC CTTGTTCGGT GATATCATCT CGGAGGTGGA GCCACGACAG 
TACCGTGGCG TTAATGGCAA CATATCAGAG GACGACCAGG GACTGCACCG CTACAGTTCG 
ACTGCTTCGA CAAATGTGGG ATCCGCTGCA GCGGTACATC GCTATCCTTC TAATGCATCG 
ACAGTGAACA GAAATGATAT CCCCGATACC TATCCCAATA CTTACGACTA CGATGACGAC 
GAGAGCGACC CCGAAGGTGC TGCTGGCCTT CTAGCCATGC AGGAGGACAT GGACGACCGA 
CGTTTTGGCT TTGGTGGGAT ATCGTTCCCG ACTTATATGG AGCCTCCGGC AGCGCAACAG 
CAGACCTCGC CGACACACCC GATACAGCCG CCGGGACACC AACGAGAGTT ACCACCACCG 
CCTGAGGAAC AAGGTACGGA AAGTGATTTT GGCGGAATGG ATCTCGGCTT GTATGGGGGT 
GGCTATGCGG GAAACCTGCA CTACGGGAAT GAAGTTGGCT CCCCACCCGC AACTTCTGCC 
CACTTTGACC AAGGGCCCAG GCCGTTGCCA TATCCGCAAA ACACGGGAAG CGAGTATGCT 
CCGTTCTCCC AAGCTGCGGT TGACTATGGT GGAACCGGTG GCCTTCAGGC CCCGCAAACA 
CACCGGCTGA GCTTCGATGA GGGAGATGAG CATGTCTCGA TTCACTCCAG GCACAGCGGA 
AGTGACTCGC CTACGAAGGA AGAGTATCCT GATATGTTCT ATCATCCTGG GATATCTAAT 
CGGCCTCTCC CGGCTGTTCC TCCTCTTGGC GAAAGCCGTC CAGTACTGTC GGTAGTGCCC 
CCTAGTAGGG TCGCTTCTCA ACAGGGGTAC AATATGGATG TAAATCAGCG CCTCATGTAT 
GACCAACAAG GTCAAGCTGC AGTGGAGAGA TCGATATCAC TTTCCAGCCA CAGCAATACC 
CCTCCTGTCC ATACACCGGC ACGATCCAGA ACCGATGCCG CTGAGGAGCG GAGGAGAGCA 
TTGAAGCATG CTAACCAGCA TCATTCTCAT CTGAGCCACC ATAGTGTGTC TCAGGATGGC 
TACGACTCTG GGACACCAAC GTCGCTTGCT GCCTATGATA TGATCACCTT GCCTGGCAGG 
AAGAGGAAGT TTATTCCATC GAAGCTCACG GCTACGGAGA TTAAGCGTTG CGCCGAACCG 
TGGGCGCTCA GTAGCGTTAC TGCCTGGATT CGTGAGATGG CAGACGGCGA AGTGGACCTC 
AAGAGAAAAT CCATCGAAGA GGGAATTGTG AAGCTTTTCT GTCACAAGGT TCCTACCATG 
AACGTTGCTG ACGCGGAAAG TTTGAGTGCT ACAGTAGTGG ACTCGATGTT TGCGTCCGGT 
CTCTTGATTC CAGATGAGGA ATGGGTCAGG TTTGGGACCG GCCACATGAC AGGTGTCTTG 
TGGCAACTCA CTGGCCATGG TTGCTACGCG CCCAAGTTGC ATGAGGCCGA GGAGTCTGCT 
CCTAAACTTA ACGATAACGG CATTCCAGTA AGGTGCTACT CGCATCACTG CGGTAGAACG 
CTGAAGAAGG CCAACCTGGA TCACCTGCTT TCTGATGATC CCGTGGTTCA GGACTGGGCG 
ACCTTCCATG GAGTCAAGCC GGAGGATTGC GAAAAGAAGA GCAAAAAGGA GATTGAGAGA 
CAAAATGTTC TGCACGAGAT TGTCACCAGT GAAGAGGAGT ACATGGCCCA GCTCGACGTT 
GTTCGGCTCC TCTACAGAGA TCAACTCCGC GTGCACCAGC CCCCTGTTCT TCCCAACAGC 
AGGAGGGACA AGTTTCTGGA AGCAGTCTTT GGTAAAGTCG ATACGGTGCA GCAAATCAAC 
AAGGACCACT TGCTTGCCCA GCTGAAGTAT CGCCAACAGG AACAGGCGCC CTTCATTGTT 
GGGTTTAGTG ACTTGTTCAG AGAGTGGATT CGCAAGGCCA GGCCCATTTA CATCGAATAC 
TGCTCGTCTT ATCCGAATTC GGAGTACCTT ATCCGAAAGG AAAGTAATAG GAATATTCTC 
TTCCGCGAGT TTCTGGGACA CGTACAAAAC CATAGACGGT CCAAGCGTTT GGGATGGACT 
ACGTTTGTGA AGGCGCCCAT CACACGTCTG CAAAGATATT CTTTGTTGCT CAGCACGGTT 
TTGAAGAACA CGATTGAAGA CAGTGAAGAG AAGCAGAACC TCATCAAGGC AATCGACGAG 
ATCAGGTCAG TCACAAAGGA GTGCGATGAC AGAGTTGCTG AGATGACCAA GAAGGTGTCC 
TTGCTTGAGC TACAACAGCA GCTAGTCCTT CGACCCGGTT TCCAATCAGT CCTCAATCTT 
GATCACCTTG GGAGAGAGCT TCTCAAGCAA GGCGAGCTTC AGCGACAGGG TTCCAAGGGA 
GTACGCTGGG TTGACACACA GGCCCTGCTG TTTGATCATT ATTTCATCTT GGCCAAAGCA 
GTCTCAACCA AGGACGGGAG GAATGAGAAG AAATACGACG TGTCGAAAGA GCCTATACCC 
ATGCCACTCC TCTTCCTGGA AAGCATGCAC GACGAACCTG TTACGAAACA AAAGGGTCTC 
GCAGCACCTC TGACCCGTAC AGCAGCTACC GCTGGATCCG GGACACAGCT TAACAAGATT 
GCCTCTAACG GAGGCGATCG GCCCGGCCTG GAGCATTCAG CAACAAGTTC GTCTATGGGA 
TCCCTCACCA CCGTTACCCG TCTAACAAGT TCAGGTGTCG ACGATGGAAA GATCATCTAC 
CCGTTTAGGA TAAAACACCT CGGCCACGAG ATTTACACGC TCTACGCTTC CTCTGCCCTG 
GACCGCTCGG AATGGTGTAA GGCGATTGTG GACGCGAAGA CAAGACACGC CGAAGCGCTA 
CATGCACAGA ACGCCGAGCC TTTCCGCTTG CGAGTTCTTT CTGACGGTGC TTTTGCGTAC 
GATTCTTATT CAACCTTTGG TAGGCAGCCG GGCGTGCCGA TTAGTGGAAC ACCGTTGGAC 
AGGGCGATAC GAGGAATGGA GGAGGTATAC GGTAAAGGTC GCGGTCCCCC GCCGGTATGC 
AGAGCTCAGG TAAACTGTGC TACCGCTTTC AAATCGTTCG GCAAGTCCAT CATCGCCATT 
GGCACGGATT ATGGCGTCTA CATCTCGGAA GCATCCAACC CCCGAGGCTG GACACGCTCT 
GTCCAGATCA GCAAGGTCAC CCAGATCGCA GTCCTCGAGG ACTTCTCTGT CTGCATTCTC 
ATCTCCGACA GGTCACTCAT CTCCTACCCC CTCGACGTGA TCGCGCCCGT CTCCAACTTT 
CCGTCCCCCG TTCATGATAA CCCCAGACGG GCTCCCCAGC GTCTTGCCAA AGATGTCACC 
TTCTTTGCCA CCGCCCGCAT GAAGGACCGC ACCCTTCTCT TCTACAAACG CAAGGAAGGC 
ATGCACAACA CCTTCAAGGT CCTCGAGCCC GTCTTCCAAA AGGCAACCGA AAAGAAATCC 
CGTATCTTCG GCAGCCGCAA ATTCGGCTCC GGCGCGACCG AGTCCTTCCG CGACTACGAC 
GAATTTTACA TACCCGCCGA GTGCTACTCG CTCAACTTGT TCCAAAGCTA CATTGCCGTG 
GCCTCCGCCA AGGGCTTTGA GCTACTGACC CTCGACAAAA AAGTAACCCA GTCCATCCCG 
CGCGACCTCA ACCTCCCCGC CATCGCCAAC ATCGCCTCCC GCATCAAGGA CCAGCGGCCC 
TTGGGCATGT TCAAGCTCAA CGACCAGGAA TTCCTTCTCA CCTACGAGGA CTGCGCCGTC 
TACGTCGACA AGCACGGCGA GATCTCCCGC ACTTTGATCA TGGAGTACTC GGGCAAACAG 
AAAAAGGCCA AAGCAGCCAC CATGTTTGGC CAGTACCTCC TCTTGTTCAA CGAGGATTAC 
GTCGAAGTCC GCAACGCCGA GAATGGGCGC TTGCGGCAGA TCATTGCCGG CCGCGATGTA 
AGGTGTTTGG ACTATGGGTT TAGAGGCCCC ACGGGCAGTG GCCAGCAGCA TGCGCAGCCG 
CATACTTTGT TGCAGGCGGG AGTGCTGCAG GATACTAAGG GGACGGTCAA GATTTGCATG 
AGTCATCCTG AGGTGCCCGG TGGACAGATT GTGTTGGAGA TGTTGCTTAA TGATGGGCAT 
GCGGAGAAGG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011392943.1
CDS	603..5837
Translation	MSFRGDDSRRYGHVPPAQYPVAGAAQDNSNSPYPPQRRMSFNTGDDAAMFDPSAARQQPAIYTGVPVVGRAEEELFLGSPTSQDAPAAQNRLSYKSQHIAMAGYQHQYQAQTSAPPPAPAHSTYNPQAFARTQSTSLPYHPAPASRYGAPTSPTYAPTPTTYTPPAYNPAAYANTNTATPQRHSSVASVASSGYNGYTYGYTSPGIPQSGFGQSPQSATAPSISSYEPARSPQYPPSSATATSPQYDQSYPPANAYNTQQYYGSYPVNASGMAYNTMTSQAPYPTAHAQMPAGPSYASADHAAFSGRNSRSDSQVSAPSPPPHSQAPGGLTRHPTNAPLPSRPMDDLPEHHSWGPNGQSNELQTQDALFGDIISEVEPRQYRGVNGNISEDDQGLHRYSSTASTNVGSAAAVHRYPSNASTVNRNDIPDTYPNTYDYDDDESDPEGAAGLLAMQEDMDDRRFGFGGISFPTYMEPPAAQQQTSPTHPIQPPGHQRELPPPPEEQGTESDFGGMDLGLYGGGYAGNLHYGNEVGSPPATSAHFDQGPRPLPYPQNTGSEYAPFSQAAVDYGGTGGLQAPQTHRLSFDEGDEHVSIHSRHSGSDSPTKEEYPDMFYHPGISNRPLPAVPPLGESRPVLSVVPPSRVASQQGYNMDVNQRLMYDQQGQAAVERSISLSSHSNTPPVHTPARSRTDAAEERRRALKHANQHHSHLSHHSVSQDGYDSGTPTSLAAYDMITLPGRKRKFIPSKLTATEIKRCAEPWALSSVTAWIREMADGEVDLKRKSIEEGIVKLFCHKVPTMNVADAESLSATVVDSMFASGLLIPDEEWVRFGTGHMTGVLWQLTGHGCYAPKLHEAEESAPKLNDNGIPVRCYSHHCGRTLKKANLDHLLSDDPVVQDWATFHGVKPEDCEKKSKKEIERQNVLHEIVTSEEEYMAQLDVVRLLYRDQLRVHQPPVLPNSRRDKFLEAVFGKVDTVQQINKDHLLAQLKYRQQEQAPFIVGFSDLFREWIRKARPIYIEYCSSYPNSEYLIRKESNRNILFREFLGHVQNHRRSKRLGWTTFVKAPITRLQRYSLLLSTVLKNTIEDSEEKQNLIKAIDEIRSVTKECDDRVAEMTKKVSLLELQQQLVLRPGFQSVLNLDHLGRELLKQGELQRQGSKGVRWVDTQALLFDHYFILAKAVSTKDGRNEKKYDVSKEPIPMPLLFLESMHDEPVTKQKGLAAPLTRTAATAGSGTQLNKIASNGGDRPGLEHSATSSSMGSLTTVTRLTSSGVDDGKIIYPFRIKHLGHEIYTLYASSALDRSEWCKAIVDAKTRHAEALHAQNAEPFRLRVLSDGAFAYDSYSTFGRQPGVPISGTPLDRAIRGMEEVYGKGRGPPPVCRAQVNCATAFKSFGKSIIAIGTDYGVYISEASNPRGWTRSVQISKVTQIAVLEDFSVCILISDRSLISYPLDVIAPVSNFPSPVHDNPRRAPQRLAKDVTFFATARMKDRTLLFYKRKEGMHNTFKVLEPVFQKATEKKSRIFGSRKFGSGATESFRDYDEFYIPAECYSLNLFQSYIAVASAKGFELLTLDKKVTQSIPRDLNLPAIANIASRIKDQRPLGMFKLNDQEFLLTYEDCAVYVDKHGEISRTLIMEYSGKQKKAKAATMFGQYLLLFNEDYVEVRNAENGRLRQIIAGRDVRCLDYGFRGPTGSGQQHAQPHTLLQAGVLQDTKGTVKICMSHPEVPGGQIVLEMLLNDGHAEKA

Target ORF information:

RefSeq Version	XM_011394641.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A rho guanyl nucleotide exchange factor (rgf3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394641.1

ORF Insert Sequence:

ATGTCGTTTC GCGGCGACGA TTCACGCCGC TATGGCCATG TGCCGCCTGC TCAATACCCC 
GTCGCCGGCG CCGCGCAGGA CAACAGCAAC TCGCCGTATC CTCCCCAGCG ACGCATGAGC 
TTCAACACGG GCGACGATGC CGCCATGTTC GACCCGAGCG CTGCCCGCCA GCAGCCCGCC 
ATTTACACTG GAGTCCCCGT TGTCGGCAGG GCCGAGGAGG AGCTCTTCCT CGGCAGCCCC 
ACGTCACAGG ATGCGCCGGC CGCCCAAAAT CGCCTCAGCT ACAAATCTCA GCACATCGCC 
ATGGCGGGCT ATCAACACCA GTATCAGGCC CAAACAAGCG CGCCGCCGCC CGCACCGGCG 
CACTCGACAT ATAACCCCCA GGCCTTTGCT CGCACTCAAT CGACCTCTCT CCCGTACCAC 
CCCGCCCCGG CCTCGAGATA CGGCGCTCCG ACGAGCCCGA CCTACGCGCC CACCCCTACA 
ACCTACACTC CGCCAGCTTA TAACCCGGCG GCGTACGCCA ACACAAATAC GGCGACGCCC 
CAGAGACATT CTTCGGTCGC TTCAGTGGCT TCCTCGGGAT ACAATGGATA CACGTATGGC 
TATACTTCTC CCGGCATCCC GCAGTCTGGG TTTGGGCAAT CTCCCCAGTC GGCTACCGCT 
CCATCCATAT CCAGCTATGA GCCTGCCCGT AGTCCACAGT ATCCTCCGTC CTCTGCCACC 
GCCACATCAC CGCAATATGA CCAATCCTAC CCCCCGGCGA ATGCGTACAA CACCCAGCAG 
TACTACGGCT CCTATCCCGT GAATGCCTCG GGCATGGCGT ATAACACCAT GACATCTCAG 
GCGCCCTATC CCACTGCACA TGCTCAGATG CCCGCCGGAC CTAGTTACGC TTCTGCGGAT 
CACGCCGCGT TTTCCGGTCG GAACTCTAGA TCAGATTCCC AGGTCTCTGC GCCATCTCCT 
CCGCCGCATT CACAAGCACC CGGGGGGCTT ACAAGACACC CGACAAATGC TCCGCTGCCT 
AGCCGTCCCA TGGATGACTT GCCAGAGCAC CATAGCTGGG GGCCCAACGG GCAAAGTAAT 
GAGCTGCAAA CCCAAGACGC CTTGTTCGGT GATATCATCT CGGAGGTGGA GCCACGACAG 
TACCGTGGCG TTAATGGCAA CATATCAGAG GACGACCAGG GACTGCACCG CTACAGTTCG 
ACTGCTTCGA CAAATGTGGG ATCCGCTGCA GCGGTACATC GCTATCCTTC TAATGCATCG 
ACAGTGAACA GAAATGATAT CCCCGATACC TATCCCAATA CTTACGACTA CGATGACGAC 
GAGAGCGACC CCGAAGGTGC TGCTGGCCTT CTAGCCATGC AGGAGGACAT GGACGACCGA 
CGTTTTGGCT TTGGTGGGAT ATCGTTCCCG ACTTATATGG AGCCTCCGGC AGCGCAACAG 
CAGACCTCGC CGACACACCC GATACAGCCG CCGGGACACC AACGAGAGTT ACCACCACCG 
CCTGAGGAAC AAGGTACGGA AAGTGATTTT GGCGGAATGG ATCTCGGCTT GTATGGGGGT 
GGCTATGCGG GAAACCTGCA CTACGGGAAT GAAGTTGGCT CCCCACCCGC AACTTCTGCC 
CACTTTGACC AAGGGCCCAG GCCGTTGCCA TATCCGCAAA ACACGGGAAG CGAGTATGCT 
CCGTTCTCCC AAGCTGCGGT TGACTATGGT GGAACCGGTG GCCTTCAGGC CCCGCAAACA 
CACCGGCTGA GCTTCGATGA GGGAGATGAG CATGTCTCGA TTCACTCCAG GCACAGCGGA 
AGTGACTCGC CTACGAAGGA AGAGTATCCT GATATGTTCT ATCATCCTGG GATATCTAAT 
CGGCCTCTCC CGGCTGTTCC TCCTCTTGGC GAAAGCCGTC CAGTACTGTC GGTAGTGCCC 
CCTAGTAGGG TCGCTTCTCA ACAGGGGTAC AATATGGATG TAAATCAGCG CCTCATGTAT 
GACCAACAAG GTCAAGCTGC AGTGGAGAGA TCGATATCAC TTTCCAGCCA CAGCAATACC 
CCTCCTGTCC ATACACCGGC ACGATCCAGA ACCGATGCCG CTGAGGAGCG GAGGAGAGCA 
TTGAAGCATG CTAACCAGCA TCATTCTCAT CTGAGCCACC ATAGTGTGTC TCAGGATGGC 
TACGACTCTG GGACACCAAC GTCGCTTGCT GCCTATGATA TGATCACCTT GCCTGGCAGG 
AAGAGGAAGT TTATTCCATC GAAGCTCACG GCTACGGAGA TTAAGCGTTG CGCCGAACCG 
TGGGCGCTCA GTAGCGTTAC TGCCTGGATT CGTGAGATGG CAGACGGCGA AGTGGACCTC 
AAGAGAAAAT CCATCGAAGA GGGAATTGTG AAGCTTTTCT GTCACAAGGT TCCTACCATG 
AACGTTGCTG ACGCGGAAAG TTTGAGTGCT ACAGTAGTGG ACTCGATGTT TGCGTCCGGT 
CTCTTGATTC CAGATGAGGA ATGGGTCAGG TTTGGGACCG GCCACATGAC AGGTGTCTTG 
TGGCAACTCA CTGGCCATGG TTGCTACGCG CCCAAGTTGC ATGAGGCCGA GGAGTCTGCT 
CCTAAACTTA ACGATAACGG CATTCCAGTA AGGTGCTACT CGCATCACTG CGGTAGAACG 
CTGAAGAAGG CCAACCTGGA TCACCTGCTT TCTGATGATC CCGTGGTTCA GGACTGGGCG 
ACCTTCCATG GAGTCAAGCC GGAGGATTGC GAAAAGAAGA GCAAAAAGGA GATTGAGAGA 
CAAAATGTTC TGCACGAGAT TGTCACCAGT GAAGAGGAGT ACATGGCCCA GCTCGACGTT 
GTTCGGCTCC TCTACAGAGA TCAACTCCGC GTGCACCAGC CCCCTGTTCT TCCCAACAGC 
AGGAGGGACA AGTTTCTGGA AGCAGTCTTT GGTAAAGTCG ATACGGTGCA GCAAATCAAC 
AAGGACCACT TGCTTGCCCA GCTGAAGTAT CGCCAACAGG AACAGGCGCC CTTCATTGTT 
GGGTTTAGTG ACTTGTTCAG AGAGTGGATT CGCAAGGCCA GGCCCATTTA CATCGAATAC 
TGCTCGTCTT ATCCGAATTC GGAGTACCTT ATCCGAAAGG AAAGTAATAG GAATATTCTC 
TTCCGCGAGT TTCTGGGACA CGTACAAAAC CATAGACGGT CCAAGCGTTT GGGATGGACT 
ACGTTTGTGA AGGCGCCCAT CACACGTCTG CAAAGATATT CTTTGTTGCT CAGCACGGTT 
TTGAAGAACA CGATTGAAGA CAGTGAAGAG AAGCAGAACC TCATCAAGGC AATCGACGAG 
ATCAGGTCAG TCACAAAGGA GTGCGATGAC AGAGTTGCTG AGATGACCAA GAAGGTGTCC 
TTGCTTGAGC TACAACAGCA GCTAGTCCTT CGACCCGGTT TCCAATCAGT CCTCAATCTT 
GATCACCTTG GGAGAGAGCT TCTCAAGCAA GGCGAGCTTC AGCGACAGGG TTCCAAGGGA 
GTACGCTGGG TTGACACACA GGCCCTGCTG TTTGATCATT ATTTCATCTT GGCCAAAGCA 
GTCTCAACCA AGGACGGGAG GAATGAGAAG AAATACGACG TGTCGAAAGA GCCTATACCC 
ATGCCACTCC TCTTCCTGGA AAGCATGCAC GACGAACCTG TTACGAAACA AAAGGGTCTC 
GCAGCACCTC TGACCCGTAC AGCAGCTACC GCTGGATCCG GGACACAGCT TAACAAGATT 
GCCTCTAACG GAGGCGATCG GCCCGGCCTG GAGCATTCAG CAACAAGTTC GTCTATGGGA 
TCCCTCACCA CCGTTACCCG TCTAACAAGT TCAGGTGTCG ACGATGGAAA GATCATCTAC 
CCGTTTAGGA TAAAACACCT CGGCCACGAG ATTTACACGC TCTACGCTTC CTCTGCCCTG 
GACCGCTCGG AATGGTGTAA GGCGATTGTG GACGCGAAGA CAAGACACGC CGAAGCGCTA 
CATGCACAGA ACGCCGAGCC TTTCCGCTTG CGAGTTCTTT CTGACGGTGC TTTTGCGTAC 
GATTCTTATT CAACCTTTGG TAGGCAGCCG GGCGTGCCGA TTAGTGGAAC ACCGTTGGAC 
AGGGCGATAC GAGGAATGGA GGAGGTATAC GGTAAAGGTC GCGGTCCCCC GCCGGTATGC 
AGAGCTCAGG TAAACTGTGC TACCGCTTTC AAATCGTTCG GCAAGTCCAT CATCGCCATT 
GGCACGGATT ATGGCGTCTA CATCTCGGAA GCATCCAACC CCCGAGGCTG GACACGCTCT 
GTCCAGATCA GCAAGGTCAC CCAGATCGCA GTCCTCGAGG ACTTCTCTGT CTGCATTCTC 
ATCTCCGACA GGTCACTCAT CTCCTACCCC CTCGACGTGA TCGCGCCCGT CTCCAACTTT 
CCGTCCCCCG TTCATGATAA CCCCAGACGG GCTCCCCAGC GTCTTGCCAA AGATGTCACC 
TTCTTTGCCA CCGCCCGCAT GAAGGACCGC ACCCTTCTCT TCTACAAACG CAAGGAAGGC 
ATGCACAACA CCTTCAAGGT CCTCGAGCCC GTCTTCCAAA AGGCAACCGA AAAGAAATCC 
CGTATCTTCG GCAGCCGCAA ATTCGGCTCC GGCGCGACCG AGTCCTTCCG CGACTACGAC 
GAATTTTACA TACCCGCCGA GTGCTACTCG CTCAACTTGT TCCAAAGCTA CATTGCCGTG 
GCCTCCGCCA AGGGCTTTGA GCTACTGACC CTCGACAAAA AAGTAACCCA GTCCATCCCG 
CGCGACCTCA ACCTCCCCGC CATCGCCAAC ATCGCCTCCC GCATCAAGGA CCAGCGGCCC 
TTGGGCATGT TCAAGCTCAA CGACCAGGAA TTCCTTCTCA CCTACGAGGA CTGCGCCGTC 
TACGTCGACA AGCACGGCGA GATCTCCCGC ACTTTGATCA TGGAGTACTC GGGCAAACAG 
AAAAAGGCCA AAGCAGCCAC CATGTTTGGC CAGTACCTCC TCTTGTTCAA CGAGGATTAC 
GTCGAAGTCC GCAACGCCGA GAATGGGCGC TTGCGGCAGA TCATTGCCGG CCGCGATGTA 
AGGTGTTTGG ACTATGGGTT TAGAGGCCCC ACGGGCAGTG GCCAGCAGCA TGCGCAGCCG 
CATACTTTGT TGCAGGCGGG AGTGCTGCAG GATACTAAGG GGACGGTCAA GATTTGCATG 
AGTCATCCTG AGGTGCCCGG TGGACAGATT GTGTTGGAGA TGTTGCTTAA TGATGGGCAT 
GCGGAGAAGG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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rgf3 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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