VIRMA cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	VIRMA
Entrez Gene ID	100924191
Full Name	vir like m6A methyltransferase associated
Synonyms	KIAA1429
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012541722.2	XP_012397176.1	protein virilizer homolog isoform X1
XM_023496392.1	XP_023352160.1	protein virilizer homolog isoform X3
XM_023496393.1	XP_023352161.1	protein virilizer homolog isoform X4
XM_023496392.2	XP_023352160.1	protein virilizer homolog isoform X2
XM_012541722.1	XP_012397176.1	protein virilizer homolog
XM_023496391.2	XP_023352159.1	protein virilizer homolog isoform X3
XM_012541722.3	XP_012397176.1	protein virilizer homolog isoform X1
XM_023496391.1	XP_023352159.1	protein virilizer homolog isoform X2

Pathways from BioSystems
Gene Ontology

The following VIRMA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VIRMA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa121922	XM_012541722.3 Latest version!	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa158743	XM_023496391.2 Latest version!	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa158744	XM_023496392.2 Latest version!	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa158745	XM_023496393.1 Latest version!	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OSa158744	XM_023496392.1	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa121922	XM_012541722.1	Sarcophilus harrisii KIAA1429 ortholog (KIAA1429), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSa121922	XM_012541722.2	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSa158743	XM_023496391.1	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VIRMA ( XM_012541722.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012397176.1 Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X1, mRNA.

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CloneID	OSa121922
Clone ID Related Accession (Same CDS sequence)	XM_012541722.2 , XM_012541722.1 , XM_012541722.3
Accession Version	XM_012541722.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein virilizer homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_012541722.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGCAGATG GTGAGAAAGA AGAGCAGTTT AATGGAAGTC CTCCAAGACC ACAGCCCCGA 
GGACCAAGGA CTCCTCCAGG ACCCCCTCCA CCTGATGATG ATGATGATGA TGAACCTCTG 
CCAGTACCAG TAGTTGGTGA GAAAGAAGAT GATGTTCCTC AACGAGAAGA TTACTTTGAG 
CCCATTTCAC CTGATCGGAC TTCTGTTCAT CAAGAAGGGC AGTATTCAGA TGAAGGTGAA 
GTAGAAGAGG ATCATCATGA GGAAGGTGAA GATGAAGAAG ATGTAGATGT TGAAGAAGAG 
GAGGATGAAG ATGAGGAAGA TGGTCATACA GTAGAGAGTA TCCCAGAAGA AGAAGAAGAT 
GAAGAAGAGG AGGAAGAAGG TGAAGAAGAA GAAGGTGAAG AAGAGGAAGA TGAAGAAGAA 
GAAGGTGAAG GAGAAGAAGA AGAAGAAGAA GAAGAAGGTG AAGGAGATGA TGGCTATGAG 
CAAATTTCTA GTGATGAAGA TGGAATTGCT GATTTAGAAC GTGAGACTTT TAAATATCCG 
AACTTTGATG TTGAATATAC TGCTGAGGAT TTAGCTTCAG TGCCTCCAAT GACATATGAT 
CCATATGACA GAGAACTTGT ACCACTTGCA TACTTTAGCT GCCCTTACAA GACAACTTTT 
GAAATTGAAG TCAGCAGGAT GAAAGACCAA GGTCCTGATA AAGAAAATTC AGGGGCAGTA 
GAAGCATCAG TAAAGTTAGT GGAACTTTTA GAGTTATACC AGGAAGATAG AGGTCCAAAA 
TGGGTCACAG CTTTGGAAGA AGTTCCAGGT TTAATAGTAA AAGGATTAAG TTATTTGCAG 
TTCAAAAATG CACAACAAGA CTACATTGGC CAGCTGGTAG ATTGGACCAT GCAAGCCTTG 
AATTTACAAG TAGCTCTCCG TCAGCCCATT GCCTTAAATG TCCGGCAGCT CAAAGCTGGG 
ACTAAATTAG TATCCTCATT AGCAGAATGT GGGCCTCAAG GAGTGATGGG ACTTTTACAA 
GCAGGAGTTA TCAGTGGATT ATTTGAACTG CTATTTGCTG ACCATGTATC ATCTTCTCTT 
AAGCTAAATG CTTTTAAAGC TTTGGACAGT ATTATTAGTA TGACTGAAGG CATGGAGGAT 
TTTTTAAGAG GCAGACAGGA CTTACATGAA AAAAGTGGTT ATCAGAAACT TCTGGAGCTC 
ATTCTCTTGG ATCAGACCGT GAGAGTAGTT ACTGCTGGTT CTGCAATTCT CCAGAAATGT 
CACTTCTATG AGATTCTGTC TGAAATTAAA AGACTTGGTG ATCAGTTGGC CGAGAGGTCT 
TCTATTCCTA ACCACAGCGA ATCTGAGCAG GACGCAGACC CTGGACTTGA GAGAACAAAT 
CCTGAATATG AAAATGAAGT GGAAGCTTCT TTGGATATAG TTCATCTTTT GGAATCCTCT 
CTTATAACTG AAGGGGAAAT AGAAAAGCTT ATTAATCTCT TGGATGAAAT CTTCCACTTA 
ATGGAGACTG CACCTCACAC GATGGTTCAG CCTCCTGTGA AGTCATTCCC GACAATAGCA 
AGGATTACTG GACCTCCAGA GAGAGATGAT CCTTATCCTG TCTTATTCAG ATACCTCCAC 
AGCCACCACT TCCTAGAATT GGTCACCTTA CTGCTGTCAA TTCCAGCAAT AAGCGCTCAC 
CCTGCTGTGC TGCAGGCTAT GAGAGATGTT TTGAAGTTTC TTACACAGTC GCAAAAAGGT 
CTTCTTTTTT TCCTCTCGGA ACATGAGGCG ACTAACTTGC TGATTAGAGC TCTTTGTCAC 
CTTCTTGATC AAGAAGAAGA AGAAGGTCTC CAGGCTGATG GTGTTATTGA TGATACATTT 
GCTTTGTGGC TACAGTATTC AACTCAGACT TTGCAGTTTA TTACAGAACT ATTTTGTCAT 
TTTCAGCGTT GCACAAACAA TGAGGAAACT GACCATTCAG ATCTCTTGGG AACCCTTCAT 
AATCTTTACC AGATTACTTT TAATCCTGTG GGAAGAGCAG CTGTTGGTCA TGTCTTCAGT 
CTTGAAAAGA ATCTCCAGAG TCTTATTACT CTAATGGAGT ACTATTCCAA AGAGGCTTTA 
GGGGACTCCA AGTCAAAGAA ATCGGTAGCT TATAATTATG CGTGCATACT TGTTTTGTTG 
GTGGTTCAGT CTTCTAGTGA TGTCCAGATG CTAGAGCAAC ATGCAGCGCC TCTCTTGAAG 
CTTTGTAAAG CAGATGAAAA TAACACGAAA TTGCAAGAAC TTGGCAAGTG GCTGGAGCCT 
CTGAAAAACC TTAGATTTGA AATTAATTGT ATCCCAAGCC TAATTGAATA TGTTAAACAG 
AATATTGATA ACTTGATGAC CCCAGAAGGA GTTGGTCTAC CAACTGCATT ACGTGTCCTC 
TGTAACATAG CGTGCCCACC ACCTCCTGTT GAAGGTCAAC AGAAAGATCT GAAGTGGAAC 
CTTGCAGTTA TTCAGCTTTT TTCTGCTGAA GGAATGGACA CCTTTATTCG TGTTCTGCAA 
AAACTGAACA ATATTCTTAC GCAGCCTTGG AGACTCCATG TCAACATGGG GACTACTCTT 
CATCGAGTTA CTACCATTTC CATGGCCCGC TGCACACTCA CTCTTCTTAA AACCATGTTA 
ACAGAGCTTC TCAGGGGTGG ATCCTTTGAG TTTAAAGACA TGCGTGTTCC TTCAGCCCTT 
GTTACTTTAC ACATGCTACT GTGCTCTATC CCTCTCTCAG GTCGTCTGGA TAGTGATGAG 
CAGAAGATTC AGAATGACAT CATTGATATC TTACTGACTT TTACGCAGGG AGTTAATGAA 
AAACTTACAA TTTCGGAAGA GACTTTAGCC AATAATACTT GGTCTTTAAT GTTAAAAGAA 
GTTCTTTCTT CAGTCTTGAG AGTTCCTGAA GGATTTTTTT CTGGACTCAT TCTGCTTTCA 
GAGCTCCTGC CCCTTCCATT GCCCATGCAA ACAACTCAGG TTATTGAGCC ACATGATATA 
TCAGTGGCAC TCAACACTCG CAAACTGTGG AGTATGCATC TCCACGTCCA GGCAAAGCTG 
CTCCAAGAAA TAGTTCGTTC TTTCTCTGGC ACAACTTGCC TGCCAATTCA GCACATGTTG 
CGGCGCATTT GTGTTCAATT ATGTGACCTT GCTTCACCAA CTGCACTTCT AATCATGAGA 
ACTGTCTTGG ATTTGATTGT AGAAGACATG CAAAGCAATT CAGAAGATAA AGAAAAACAG 
TATACAAGTC AAACAACCAG ACTTCTGGCT CTTCTTGATG CCCTGGCTTC ACACAAAGCT 
TGTAAATTGG CTATTTTGCA TCTGATAAAT GGAACTATTA AAGGTGATGA ACGATATGCA 
GAGATTTTCC AGGAATTGTT AGCTCTTCGA TCAACTGGAG AGAGTGTTAT TCATCAACAG 
TGTGTTGAAT ATGTCATGTC CATTTTGCAG TCTCTCTGTG ATCAGGACAT AGCACTTATT 
TTGCCAAGTT CTTCAGAAGG ATCACTTTCA GAACTGGAAC AGTTGTCCAA TTCTTTACCA 
AACAAGGAAT TGATGGTCTT GATCTGTGAC TGTCTACTGG AAACAGTAGC CAATTCTGAG 
AATAGTTACA ACTGCTTACT GACATGTGTC AGAACTATGA TGTTTCTTGC AGAACATGAT 
TATGGATTCT TTCACTTAAA AAGTTCATTA AGAAAACACA GTAATGCTTT GCACCGATTA 
TTGAAACGCA TTGTTAGTAC ATTTAGTAAG GATACAGGAG AACTTGCATC TTCTTTTTTG 
GAATTCATGA GACAAATACT CAACTCTGAC ACAATGGGAT GTTGTGGAGA TGACAGTGGT 
CTCATGGAAA TAGATGGATC TCATTCATCA AGAACATTGA GTCTTAATGC TGCAGAATTA 
AAACAACTTT TACAGAGCAA AGAAGAAAGT CCAGAAAGTT TGCTTCTTCA ACTGGAGAAG 
CATATTTTGG AACATTCTAA AGAGGATGAC AACCTGGATT CTTTGCAGGA CAACATAGTT 
GGTCTTAGAC AAATGCTGGA ATCTGCAGGT GATCCTTTAC CACTTAGTGA TCAAGATGTG 
GAACCAGTAC TTTCAGCTCC AGAATCTCTT CAAAATCTCT TTAATAATAG GACTGCATAT 
GTGCTAGCTG ATGTCATGGA TGACCAGCTG AAATCCATGT GGTTCTCTCC ATTCCAGGCT 
GAAGAGATTG AAACAGATTT AGATTTGGTA AAAGTTGATC TAATTGAGCT GTCTGAAAAA 
TGTTATAGTG ACTTTGATTT GCAAGCCGAA TTAGAGCGTT CCTTTTTGTC AGAACCATCA 
TCTCCTGGGA GAACAAAGAC TACAAAAGGA TTTAAACTTG GCAAGCACAA ACATGAGACT 
TTTATAACAT CGAGTGGAAA ATCTGAATAC ATTGAACCTG CCAAGAGAGC ACACATTGTG 
CCACCTCCCA GAGGAAGAGG CCGGGGAGGA TTTGGACAGG GCATCAGACC CCATGATATC 
TTTCGTCAGA GGAAACAGAA CACCAGTAGG CCACCATCTA TGCACGTGGA TGATTTTGTT 
GCAGCTGAAA GCAAAGAAGT GGTCCCACAA GATGGGTTAC CACCACCAAA AAGGCCACCC 
AAAGTATCAC AGAAGATTTC TTCTCGTGGT GGATTTTCAG GGAACCGAGG AGGACGAGGT 
GCTTTTCATA GTCAGAATCG GTTCTTCACT CCACCTGCTT CAAAAGGAAA TTATAGTCGT 
CGTGAGGGAA CACGAGGTTC TAGCTGGAGT GCGCAGAGTA CACCACGAGG AACCTATAAT 
GAAAGTAGAG GAGGGCAGAG CAATTTTAGC AGGGGGCCTC TTCCACCCCT TCGGCCACTC 
AGTTCTGCAG GATATCGTCC AAGTCCACGC GACCGTGCTT CCAGAGGTCG GGGTGGACTT 
GGTCCTTCTT GGGCAAGCGC AAATAGTGGC AGTGGAGGCT CAAGAGGAAA ATTTGTTAGT 
GGAGGCAGCG GGAGAGGTCG TCATGTACGG TCCTTCACAC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012397176.1
CDS	41..5545
Translation	MAVDSAMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSSLPDNRAYGETSPHTFQLDFFFNNVSKPSAPVFDRLGSLDYDENTSIIFRPNAKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQQQPSVKRTPKHADGEKEEQFNGSPPRPQPRGPRTPPGPPPPDDDDDDEPLPVPVVGEKEDDVPQREDYFEPISPDRTSVHQEGQYSDEGEVEEDHHEEGEDEEDVDVEEEEDEDEEDGHTVESIPEEEEDEEEEEEGEEEEGEEEEDEEEEGEGEEEEEEEEGEGDDGYEQISSDEDGIADLERETFKYPNFDVEYTAEDLASVPPMTYDPYDRELVPLAYFSCPYKTTFEIEVSRMKDQGPDKENSGAVEASVKLVELLELYQEDRGPKWVTALEEVPGLIVKGLSYLQFKNAQQDYIGQLVDWTMQALNLQVALRQPIALNVRQLKAGTKLVSSLAECGPQGVMGLLQAGVISGLFELLFADHVSSSLKLNAFKALDSIISMTEGMEDFLRGRQDLHEKSGYQKLLELILLDQTVRVVTAGSAILQKCHFYEILSEIKRLGDQLAERSSIPNHSESEQDADPGLERTNPEYENEVEASLDIVHLLESSLITEGEIEKLINLLDEIFHLMETAPHTMVQPPVKSFPTIARITGPPERDDPYPVLFRYLHSHHFLELVTLLLSIPAISAHPAVLQAMRDVLKFLTQSQKGLLFFLSEHEATNLLIRALCHLLDQEEEEGLQADGVIDDTFALWLQYSTQTLQFITELFCHFQRCTNNEETDHSDLLGTLHNLYQITFNPVGRAAVGHVFSLEKNLQSLITLMEYYSKEALGDSKSKKSVAYNYACILVLLVVQSSSDVQMLEQHAAPLLKLCKADENNTKLQELGKWLEPLKNLRFEINCIPSLIEYVKQNIDNLMTPEGVGLPTALRVLCNIACPPPPVEGQQKDLKWNLAVIQLFSAEGMDTFIRVLQKLNNILTQPWRLHVNMGTTLHRVTTISMARCTLTLLKTMLTELLRGGSFEFKDMRVPSALVTLHMLLCSIPLSGRLDSDEQKIQNDIIDILLTFTQGVNEKLTISEETLANNTWSLMLKEVLSSVLRVPEGFFSGLILLSELLPLPLPMQTTQVIEPHDISVALNTRKLWSMHLHVQAKLLQEIVRSFSGTTCLPIQHMLRRICVQLCDLASPTALLIMRTVLDLIVEDMQSNSEDKEKQYTSQTTRLLALLDALASHKACKLAILHLINGTIKGDERYAEIFQELLALRSTGESVIHQQCVEYVMSILQSLCDQDIALILPSSSEGSLSELEQLSNSLPNKELMVLICDCLLETVANSENSYNCLLTCVRTMMFLAEHDYGFFHLKSSLRKHSNALHRLLKRIVSTFSKDTGELASSFLEFMRQILNSDTMGCCGDDSGLMEIDGSHSSRTLSLNAAELKQLLQSKEESPESLLLQLEKHILEHSKEDDNLDSLQDNIVGLRQMLESAGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFSPFQAEEIETDLDLVKVDLIELSEKCYSDFDLQAELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHIVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGLPPPKRPPKVSQKISSRGGFSGNRGGRGAFHSQNRFFTPPASKGNYSRREGTRGSSWSAQSTPRGTYNESRGGQSNFSRGPLPPLRPLSSAGYRPSPRDRASRGRGGLGPSWASANSGSGGSRGKFVSGGSGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_012541722.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012541722.3

ORF Insert Sequence:

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGCAGATG GTGAGAAAGA AGAGCAGTTT AATGGAAGTC CTCCAAGACC ACAGCCCCGA 
GGACCAAGGA CTCCTCCAGG ACCCCCTCCA CCTGATGATG ATGATGATGA TGAACCTCTG 
CCAGTACCAG TAGTTGGTGA GAAAGAAGAT GATGTTCCTC AACGAGAAGA TTACTTTGAG 
CCCATTTCAC CTGATCGGAC TTCTGTTCAT CAAGAAGGGC AGTATTCAGA TGAAGGTGAA 
GTAGAAGAGG ATCATCATGA GGAAGGTGAA GATGAAGAAG ATGTAGATGT TGAAGAAGAG 
GAGGATGAAG ATGAGGAAGA TGGTCATACA GTAGAGAGTA TCCCAGAAGA AGAAGAAGAT 
GAAGAAGAGG AGGAAGAAGG TGAAGAAGAA GAAGGTGAAG AAGAGGAAGA TGAAGAAGAA 
GAAGGTGAAG GAGAAGAAGA AGAAGAAGAA GAAGAAGGTG AAGGAGATGA TGGCTATGAG 
CAAATTTCTA GTGATGAAGA TGGAATTGCT GATTTAGAAC GTGAGACTTT TAAATATCCG 
AACTTTGATG TTGAATATAC TGCTGAGGAT TTAGCTTCAG TGCCTCCAAT GACATATGAT 
CCATATGACA GAGAACTTGT ACCACTTGCA TACTTTAGCT GCCCTTACAA GACAACTTTT 
GAAATTGAAG TCAGCAGGAT GAAAGACCAA GGTCCTGATA AAGAAAATTC AGGGGCAGTA 
GAAGCATCAG TAAAGTTAGT GGAACTTTTA GAGTTATACC AGGAAGATAG AGGTCCAAAA 
TGGGTCACAG CTTTGGAAGA AGTTCCAGGT TTAATAGTAA AAGGATTAAG TTATTTGCAG 
TTCAAAAATG CACAACAAGA CTACATTGGC CAGCTGGTAG ATTGGACCAT GCAAGCCTTG 
AATTTACAAG TAGCTCTCCG TCAGCCCATT GCCTTAAATG TCCGGCAGCT CAAAGCTGGG 
ACTAAATTAG TATCCTCATT AGCAGAATGT GGGCCTCAAG GAGTGATGGG ACTTTTACAA 
GCAGGAGTTA TCAGTGGATT ATTTGAACTG CTATTTGCTG ACCATGTATC ATCTTCTCTT 
AAGCTAAATG CTTTTAAAGC TTTGGACAGT ATTATTAGTA TGACTGAAGG CATGGAGGAT 
TTTTTAAGAG GCAGACAGGA CTTACATGAA AAAAGTGGTT ATCAGAAACT TCTGGAGCTC 
ATTCTCTTGG ATCAGACCGT GAGAGTAGTT ACTGCTGGTT CTGCAATTCT CCAGAAATGT 
CACTTCTATG AGATTCTGTC TGAAATTAAA AGACTTGGTG ATCAGTTGGC CGAGAGGTCT 
TCTATTCCTA ACCACAGCGA ATCTGAGCAG GACGCAGACC CTGGACTTGA GAGAACAAAT 
CCTGAATATG AAAATGAAGT GGAAGCTTCT TTGGATATAG TTCATCTTTT GGAATCCTCT 
CTTATAACTG AAGGGGAAAT AGAAAAGCTT ATTAATCTCT TGGATGAAAT CTTCCACTTA 
ATGGAGACTG CACCTCACAC GATGGTTCAG CCTCCTGTGA AGTCATTCCC GACAATAGCA 
AGGATTACTG GACCTCCAGA GAGAGATGAT CCTTATCCTG TCTTATTCAG ATACCTCCAC 
AGCCACCACT TCCTAGAATT GGTCACCTTA CTGCTGTCAA TTCCAGCAAT AAGCGCTCAC 
CCTGCTGTGC TGCAGGCTAT GAGAGATGTT TTGAAGTTTC TTACACAGTC GCAAAAAGGT 
CTTCTTTTTT TCCTCTCGGA ACATGAGGCG ACTAACTTGC TGATTAGAGC TCTTTGTCAC 
CTTCTTGATC AAGAAGAAGA AGAAGGTCTC CAGGCTGATG GTGTTATTGA TGATACATTT 
GCTTTGTGGC TACAGTATTC AACTCAGACT TTGCAGTTTA TTACAGAACT ATTTTGTCAT 
TTTCAGCGTT GCACAAACAA TGAGGAAACT GACCATTCAG ATCTCTTGGG AACCCTTCAT 
AATCTTTACC AGATTACTTT TAATCCTGTG GGAAGAGCAG CTGTTGGTCA TGTCTTCAGT 
CTTGAAAAGA ATCTCCAGAG TCTTATTACT CTAATGGAGT ACTATTCCAA AGAGGCTTTA 
GGGGACTCCA AGTCAAAGAA ATCGGTAGCT TATAATTATG CGTGCATACT TGTTTTGTTG 
GTGGTTCAGT CTTCTAGTGA TGTCCAGATG CTAGAGCAAC ATGCAGCGCC TCTCTTGAAG 
CTTTGTAAAG CAGATGAAAA TAACACGAAA TTGCAAGAAC TTGGCAAGTG GCTGGAGCCT 
CTGAAAAACC TTAGATTTGA AATTAATTGT ATCCCAAGCC TAATTGAATA TGTTAAACAG 
AATATTGATA ACTTGATGAC CCCAGAAGGA GTTGGTCTAC CAACTGCATT ACGTGTCCTC 
TGTAACATAG CGTGCCCACC ACCTCCTGTT GAAGGTCAAC AGAAAGATCT GAAGTGGAAC 
CTTGCAGTTA TTCAGCTTTT TTCTGCTGAA GGAATGGACA CCTTTATTCG TGTTCTGCAA 
AAACTGAACA ATATTCTTAC GCAGCCTTGG AGACTCCATG TCAACATGGG GACTACTCTT 
CATCGAGTTA CTACCATTTC CATGGCCCGC TGCACACTCA CTCTTCTTAA AACCATGTTA 
ACAGAGCTTC TCAGGGGTGG ATCCTTTGAG TTTAAAGACA TGCGTGTTCC TTCAGCCCTT 
GTTACTTTAC ACATGCTACT GTGCTCTATC CCTCTCTCAG GTCGTCTGGA TAGTGATGAG 
CAGAAGATTC AGAATGACAT CATTGATATC TTACTGACTT TTACGCAGGG AGTTAATGAA 
AAACTTACAA TTTCGGAAGA GACTTTAGCC AATAATACTT GGTCTTTAAT GTTAAAAGAA 
GTTCTTTCTT CAGTCTTGAG AGTTCCTGAA GGATTTTTTT CTGGACTCAT TCTGCTTTCA 
GAGCTCCTGC CCCTTCCATT GCCCATGCAA ACAACTCAGG TTATTGAGCC ACATGATATA 
TCAGTGGCAC TCAACACTCG CAAACTGTGG AGTATGCATC TCCACGTCCA GGCAAAGCTG 
CTCCAAGAAA TAGTTCGTTC TTTCTCTGGC ACAACTTGCC TGCCAATTCA GCACATGTTG 
CGGCGCATTT GTGTTCAATT ATGTGACCTT GCTTCACCAA CTGCACTTCT AATCATGAGA 
ACTGTCTTGG ATTTGATTGT AGAAGACATG CAAAGCAATT CAGAAGATAA AGAAAAACAG 
TATACAAGTC AAACAACCAG ACTTCTGGCT CTTCTTGATG CCCTGGCTTC ACACAAAGCT 
TGTAAATTGG CTATTTTGCA TCTGATAAAT GGAACTATTA AAGGTGATGA ACGATATGCA 
GAGATTTTCC AGGAATTGTT AGCTCTTCGA TCAACTGGAG AGAGTGTTAT TCATCAACAG 
TGTGTTGAAT ATGTCATGTC CATTTTGCAG TCTCTCTGTG ATCAGGACAT AGCACTTATT 
TTGCCAAGTT CTTCAGAAGG ATCACTTTCA GAACTGGAAC AGTTGTCCAA TTCTTTACCA 
AACAAGGAAT TGATGGTCTT GATCTGTGAC TGTCTACTGG AAACAGTAGC CAATTCTGAG 
AATAGTTACA ACTGCTTACT GACATGTGTC AGAACTATGA TGTTTCTTGC AGAACATGAT 
TATGGATTCT TTCACTTAAA AAGTTCATTA AGAAAACACA GTAATGCTTT GCACCGATTA 
TTGAAACGCA TTGTTAGTAC ATTTAGTAAG GATACAGGAG AACTTGCATC TTCTTTTTTG 
GAATTCATGA GACAAATACT CAACTCTGAC ACAATGGGAT GTTGTGGAGA TGACAGTGGT 
CTCATGGAAA TAGATGGATC TCATTCATCA AGAACATTGA GTCTTAATGC TGCAGAATTA 
AAACAACTTT TACAGAGCAA AGAAGAAAGT CCAGAAAGTT TGCTTCTTCA ACTGGAGAAG 
CATATTTTGG AACATTCTAA AGAGGATGAC AACCTGGATT CTTTGCAGGA CAACATAGTT 
GGTCTTAGAC AAATGCTGGA ATCTGCAGGT GATCCTTTAC CACTTAGTGA TCAAGATGTG 
GAACCAGTAC TTTCAGCTCC AGAATCTCTT CAAAATCTCT TTAATAATAG GACTGCATAT 
GTGCTAGCTG ATGTCATGGA TGACCAGCTG AAATCCATGT GGTTCTCTCC ATTCCAGGCT 
GAAGAGATTG AAACAGATTT AGATTTGGTA AAAGTTGATC TAATTGAGCT GTCTGAAAAA 
TGTTATAGTG ACTTTGATTT GCAAGCCGAA TTAGAGCGTT CCTTTTTGTC AGAACCATCA 
TCTCCTGGGA GAACAAAGAC TACAAAAGGA TTTAAACTTG GCAAGCACAA ACATGAGACT 
TTTATAACAT CGAGTGGAAA ATCTGAATAC ATTGAACCTG CCAAGAGAGC ACACATTGTG 
CCACCTCCCA GAGGAAGAGG CCGGGGAGGA TTTGGACAGG GCATCAGACC CCATGATATC 
TTTCGTCAGA GGAAACAGAA CACCAGTAGG CCACCATCTA TGCACGTGGA TGATTTTGTT 
GCAGCTGAAA GCAAAGAAGT GGTCCCACAA GATGGGTTAC CACCACCAAA AAGGCCACCC 
AAAGTATCAC AGAAGATTTC TTCTCGTGGT GGATTTTCAG GGAACCGAGG AGGACGAGGT 
GCTTTTCATA GTCAGAATCG GTTCTTCACT CCACCTGCTT CAAAAGGAAA TTATAGTCGT 
CGTGAGGGAA CACGAGGTTC TAGCTGGAGT GCGCAGAGTA CACCACGAGG AACCTATAAT 
GAAAGTAGAG GAGGGCAGAG CAATTTTAGC AGGGGGCCTC TTCCACCCCT TCGGCCACTC 
AGTTCTGCAG GATATCGTCC AAGTCCACGC GACCGTGCTT CCAGAGGTCG GGGTGGACTT 
GGTCCTTCTT GGGCAAGCGC AAATAGTGGC AGTGGAGGCT CAAGAGGAAA ATTTGTTAGT 
GGAGGCAGCG GGAGAGGTCG TCATGTACGG TCCTTCACAC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VIRMA ( XM_023496391.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023352159.1 Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X3, mRNA.

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CloneID	OSa158743
Clone ID Related Accession (Same CDS sequence)	XM_023496391.2 , XM_023496391.1
Accession Version	XM_023496391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein virilizer homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023496391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGCAGATG GTGAGAAAGA AGAGCAGTTT AATGGAAGTC CTCCAAGACC ACAGCCCCGA 
GGACCAAGGA CTCCTCCAGG ACCCCCTCCA CCTGATGATG ATGATGATGA TGAACCTCTG 
CCAGTACCAG TTGGTGAGAA AGAAGATGAT GTTCCTCAAC GAGAAGATTA CTTTGAGCCC 
ATTTCACCTG ATCGGACTTC TGTTCATCAA GAAGGGCAGT ATTCAGATGA AGGTGAAGTA 
GAAGAGGATC ATCATGAGGA AGGTGAAGAT GAAGAAGATG TAGATGTTGA AGAAGAGGAG 
GATGAAGATG AGGAAGATGG TCATACAGTA GAGAGTATCC CAGAAGAAGA AGAAGATGAA 
GAAGAGGAGG AAGAAGGTGA AGAAGAAGAA GGTGAAGAAG AGGAAGATGA AGAAGAAGAA 
GGTGAAGGAG AAGAAGAAGA AGAAGAAGAA GAAGGTGAAG GAGATGATGG CTATGAGCAA 
ATTTCTAGTG ATGAAGATGG AATTGCTGAT TTAGAACGTG AGACTTTTAA ATATCCGAAC 
TTTGATGTTG AATATACTGC TGAGGATTTA GCTTCAGTGC CTCCAATGAC ATATGATCCA 
TATGACAGAG AACTTGTACC ACTTGCATAC TTTAGCTGCC CTTACAAGAC AACTTTTGAA 
ATTGAAGTCA GCAGGATGAA AGACCAAGGT CCTGATAAAG AAAATTCAGG GGCAGTAGAA 
GCATCAGTAA AGTTAGTGGA ACTTTTAGAG TTATACCAGG AAGATAGAGG TCCAAAATGG 
GTCACAGCTT TGGAAGAAGT TCCAGGTTTA ATAGTAAAAG GATTAAGTTA TTTGCAGTTC 
AAAAATGCAC AACAAGACTA CATTGGCCAG CTGGTAGATT GGACCATGCA AGCCTTGAAT 
TTACAAGTAG CTCTCCGTCA GCCCATTGCC TTAAATGTCC GGCAGCTCAA AGCTGGGACT 
AAATTAGTAT CCTCATTAGC AGAATGTGGG CCTCAAGGAG TGATGGGACT TTTACAAGCA 
GGAGTTATCA GTGGATTATT TGAACTGCTA TTTGCTGACC ATGTATCATC TTCTCTTAAG 
CTAAATGCTT TTAAAGCTTT GGACAGTATT ATTAGTATGA CTGAAGGCAT GGAGGATTTT 
TTAAGAGGCA GACAGGACTT ACATGAAAAA AGTGGTTATC AGAAACTTCT GGAGCTCATT 
CTCTTGGATC AGACCGTGAG AGTAGTTACT GCTGGTTCTG CAATTCTCCA GAAATGTCAC 
TTCTATGAGA TTCTGTCTGA AATTAAAAGA CTTGGTGATC AGTTGGCCGA GAGGTCTTCT 
ATTCCTAACC ACAGCGAATC TGAGCAGGAC GCAGACCCTG GACTTGAGAG AACAAATCCT 
GAATATGAAA ATGAAGTGGA AGCTTCTTTG GATATAGTTC ATCTTTTGGA ATCCTCTCTT 
ATAACTGAAG GGGAAATAGA AAAGCTTATT AATCTCTTGG ATGAAATCTT CCACTTAATG 
GAGACTGCAC CTCACACGAT GGTTCAGCCT CCTGTGAAGT CATTCCCGAC AATAGCAAGG 
ATTACTGGAC CTCCAGAGAG AGATGATCCT TATCCTGTCT TATTCAGATA CCTCCACAGC 
CACCACTTCC TAGAATTGGT CACCTTACTG CTGTCAATTC CAGCAATAAG CGCTCACCCT 
GCTGTGCTGC AGGCTATGAG AGATGTTTTG AAGTTTCTTA CACAGTCGCA AAAAGGTCTT 
CTTTTTTTCC TCTCGGAACA TGAGGCGACT AACTTGCTGA TTAGAGCTCT TTGTCACCTT 
CTTGATCAAG AAGAAGAAGA AGGTCTCCAG GCTGATGGTG TTATTGATGA TACATTTGCT 
TTGTGGCTAC AGTATTCAAC TCAGACTTTG CAGTTTATTA CAGAACTATT TTGTCATTTT 
CAGCGTTGCA CAAACAATGA GGAAACTGAC CATTCAGATC TCTTGGGAAC CCTTCATAAT 
CTTTACCAGA TTACTTTTAA TCCTGTGGGA AGAGCAGCTG TTGGTCATGT CTTCAGTCTT 
GAAAAGAATC TCCAGAGTCT TATTACTCTA ATGGAGTACT ATTCCAAAGA GGCTTTAGGG 
GACTCCAAGT CAAAGAAATC GGTAGCTTAT AATTATGCGT GCATACTTGT TTTGTTGGTG 
GTTCAGTCTT CTAGTGATGT CCAGATGCTA GAGCAACATG CAGCGCCTCT CTTGAAGCTT 
TGTAAAGCAG ATGAAAATAA CACGAAATTG CAAGAACTTG GCAAGTGGCT GGAGCCTCTG 
AAAAACCTTA GATTTGAAAT TAATTGTATC CCAAGCCTAA TTGAATATGT TAAACAGAAT 
ATTGATAACT TGATGACCCC AGAAGGAGTT GGTCTACCAA CTGCATTACG TGTCCTCTGT 
AACATAGCGT GCCCACCACC TCCTGTTGAA GGTCAACAGA AAGATCTGAA GTGGAACCTT 
GCAGTTATTC AGCTTTTTTC TGCTGAAGGA ATGGACACCT TTATTCGTGT TCTGCAAAAA 
CTGAACAATA TTCTTACGCA GCCTTGGAGA CTCCATGTCA ACATGGGGAC TACTCTTCAT 
CGAGTTACTA CCATTTCCAT GGCCCGCTGC ACACTCACTC TTCTTAAAAC CATGTTAACA 
GAGCTTCTCA GGGGTGGATC CTTTGAGTTT AAAGACATGC GTGTTCCTTC AGCCCTTGTT 
ACTTTACACA TGCTACTGTG CTCTATCCCT CTCTCAGGTC GTCTGGATAG TGATGAGCAG 
AAGATTCAGA ATGACATCAT TGATATCTTA CTGACTTTTA CGCAGGGAGT TAATGAAAAA 
CTTACAATTT CGGAAGAGAC TTTAGCCAAT AATACTTGGT CTTTAATGTT AAAAGAAGTT 
CTTTCTTCAG TCTTGAGAGT TCCTGAAGGA TTTTTTTCTG GACTCATTCT GCTTTCAGAG 
CTCCTGCCCC TTCCATTGCC CATGCAAACA ACTCAGGTTA TTGAGCCACA TGATATATCA 
GTGGCACTCA ACACTCGCAA ACTGTGGAGT ATGCATCTCC ACGTCCAGGC AAAGCTGCTC 
CAAGAAATAG TTCGTTCTTT CTCTGGCACA ACTTGCCTGC CAATTCAGCA CATGTTGCGG 
CGCATTTGTG TTCAATTATG TGACCTTGCT TCACCAACTG CACTTCTAAT CATGAGAACT 
GTCTTGGATT TGATTGTAGA AGACATGCAA AGCAATTCAG AAGATAAAGA AAAACAGTAT 
ACAAGTCAAA CAACCAGACT TCTGGCTCTT CTTGATGCCC TGGCTTCACA CAAAGCTTGT 
AAATTGGCTA TTTTGCATCT GATAAATGGA ACTATTAAAG GTGATGAACG ATATGCAGAG 
ATTTTCCAGG AATTGTTAGC TCTTCGATCA ACTGGAGAGA GTGTTATTCA TCAACAGTGT 
GTTGAATATG TCATGTCCAT TTTGCAGTCT CTCTGTGATC AGGACATAGC ACTTATTTTG 
CCAAGTTCTT CAGAAGGATC ACTTTCAGAA CTGGAACAGT TGTCCAATTC TTTACCAAAC 
AAGGAATTGA TGGTCTTGAT CTGTGACTGT CTACTGGAAA CAGTAGCCAA TTCTGAGAAT 
AGTTACAACT GCTTACTGAC ATGTGTCAGA ACTATGATGT TTCTTGCAGA ACATGATTAT 
GGATTCTTTC ACTTAAAAAG TTCATTAAGA AAACACAGTA ATGCTTTGCA CCGATTATTG 
AAACGCATTG TTAGTACATT TAGTAAGGAT ACAGGAGAAC TTGCATCTTC TTTTTTGGAA 
TTCATGAGAC AAATACTCAA CTCTGACACA ATGGGATGTT GTGGAGATGA CAGTGGTCTC 
ATGGAAATAG ATGGATCTCA TTCATCAAGA ACATTGAGTC TTAATGCTGC AGAATTAAAA 
CAACTTTTAC AGAGCAAAGA AGAAAGTCCA GAAAGTTTGC TTCTTCAACT GGAGAAGCAT 
ATTTTGGAAC ATTCTAAAGA GGATGACAAC CTGGATTCTT TGCAGGACAA CATAGTTGGT 
CTTAGACAAA TGCTGGAATC TGCAGGTGAT CCTTTACCAC TTAGTGATCA AGATGTGGAA 
CCAGTACTTT CAGCTCCAGA ATCTCTTCAA AATCTCTTTA ATAATAGGAC TGCATATGTG 
CTAGCTGATG TCATGGATGA CCAGCTGAAA TCCATGTGGT TCTCTCCATT CCAGGCTGAA 
GAGATTGAAA CAGATTTAGA TTTGGTAAAA GTTGATCTAA TTGAGCTGTC TGAAAAATGT 
TATAGTGACT TTGATTTGCA AGCCGAATTA GAGCGTTCCT TTTTGTCAGA ACCATCATCT 
CCTGGGAGAA CAAAGACTAC AAAAGGATTT AAACTTGGCA AGCACAAACA TGAGACTTTT 
ATAACATCGA GTGGAAAATC TGAATACATT GAACCTGCCA AGAGAGCACA CATTGTGCCA 
CCTCCCAGAG GAAGAGGCCG GGGAGGATTT GGACAGGGCA TCAGACCCCA TGATATCTTT 
CGTCAGAGGA AACAGAACAC CAGTAGGCCA CCATCTATGC ACGTGGATGA TTTTGTTGCA 
GCTGAAAGCA AAGAAGTGGT CCCACAAGAT GGGTTACCAC CACCAAAAAG GCCACCCAAA 
GTATCACAGA AGATTTCTTC TCGTGGTGGA TTTTCAGGGA ACCGAGGAGG ACGAGGTGCT 
TTTCATAGTC AGAATCGGTT CTTCACTCCA CCTGCTTCAA AAGGAAATTA TAGTCGTCGT 
GAGGGAACAC GAGGTTCTAG CTGGAGTGCG CAGAGTACAC CACGAGGAAC CTATAATGAA 
AGTAGAGGAG GGCAGAGCAA TTTTAGCAGG GGGCCTCTTC CACCCCTTCG GCCACTCAGT 
TCTGCAGGAT ATCGTCCAAG TCCACGCGAC CGTGCTTCCA GAGGTCGGGG TGGACTTGGT 
CCTTCTTGGG CAAGCGCAAA TAGTGGCAGT GGAGGCTCAA GAGGAAAATT TGTTAGTGGA 
GGCAGCGGGA GAGGTCGTCA TGTACGGTCC TTCACACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023352159.1
CDS	41..5542
Translation	MAVDSAMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSSLPDNRAYGETSPHTFQLDFFFNNVSKPSAPVFDRLGSLDYDENTSIIFRPNAKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQQQPSVKRTPKHADGEKEEQFNGSPPRPQPRGPRTPPGPPPPDDDDDDEPLPVPVGEKEDDVPQREDYFEPISPDRTSVHQEGQYSDEGEVEEDHHEEGEDEEDVDVEEEEDEDEEDGHTVESIPEEEEDEEEEEEGEEEEGEEEEDEEEEGEGEEEEEEEEGEGDDGYEQISSDEDGIADLERETFKYPNFDVEYTAEDLASVPPMTYDPYDRELVPLAYFSCPYKTTFEIEVSRMKDQGPDKENSGAVEASVKLVELLELYQEDRGPKWVTALEEVPGLIVKGLSYLQFKNAQQDYIGQLVDWTMQALNLQVALRQPIALNVRQLKAGTKLVSSLAECGPQGVMGLLQAGVISGLFELLFADHVSSSLKLNAFKALDSIISMTEGMEDFLRGRQDLHEKSGYQKLLELILLDQTVRVVTAGSAILQKCHFYEILSEIKRLGDQLAERSSIPNHSESEQDADPGLERTNPEYENEVEASLDIVHLLESSLITEGEIEKLINLLDEIFHLMETAPHTMVQPPVKSFPTIARITGPPERDDPYPVLFRYLHSHHFLELVTLLLSIPAISAHPAVLQAMRDVLKFLTQSQKGLLFFLSEHEATNLLIRALCHLLDQEEEEGLQADGVIDDTFALWLQYSTQTLQFITELFCHFQRCTNNEETDHSDLLGTLHNLYQITFNPVGRAAVGHVFSLEKNLQSLITLMEYYSKEALGDSKSKKSVAYNYACILVLLVVQSSSDVQMLEQHAAPLLKLCKADENNTKLQELGKWLEPLKNLRFEINCIPSLIEYVKQNIDNLMTPEGVGLPTALRVLCNIACPPPPVEGQQKDLKWNLAVIQLFSAEGMDTFIRVLQKLNNILTQPWRLHVNMGTTLHRVTTISMARCTLTLLKTMLTELLRGGSFEFKDMRVPSALVTLHMLLCSIPLSGRLDSDEQKIQNDIIDILLTFTQGVNEKLTISEETLANNTWSLMLKEVLSSVLRVPEGFFSGLILLSELLPLPLPMQTTQVIEPHDISVALNTRKLWSMHLHVQAKLLQEIVRSFSGTTCLPIQHMLRRICVQLCDLASPTALLIMRTVLDLIVEDMQSNSEDKEKQYTSQTTRLLALLDALASHKACKLAILHLINGTIKGDERYAEIFQELLALRSTGESVIHQQCVEYVMSILQSLCDQDIALILPSSSEGSLSELEQLSNSLPNKELMVLICDCLLETVANSENSYNCLLTCVRTMMFLAEHDYGFFHLKSSLRKHSNALHRLLKRIVSTFSKDTGELASSFLEFMRQILNSDTMGCCGDDSGLMEIDGSHSSRTLSLNAAELKQLLQSKEESPESLLLQLEKHILEHSKEDDNLDSLQDNIVGLRQMLESAGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFSPFQAEEIETDLDLVKVDLIELSEKCYSDFDLQAELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHIVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGLPPPKRPPKVSQKISSRGGFSGNRGGRGAFHSQNRFFTPPASKGNYSRREGTRGSSWSAQSTPRGTYNESRGGQSNFSRGPLPPLRPLSSAGYRPSPRDRASRGRGGLGPSWASANSGSGGSRGKFVSGGSGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_023496391.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023496391.2

ORF Insert Sequence:

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGCAGATG GTGAGAAAGA AGAGCAGTTT AATGGAAGTC CTCCAAGACC ACAGCCCCGA 
GGACCAAGGA CTCCTCCAGG ACCCCCTCCA CCTGATGATG ATGATGATGA TGAACCTCTG 
CCAGTACCAG TTGGTGAGAA AGAAGATGAT GTTCCTCAAC GAGAAGATTA CTTTGAGCCC 
ATTTCACCTG ATCGGACTTC TGTTCATCAA GAAGGGCAGT ATTCAGATGA AGGTGAAGTA 
GAAGAGGATC ATCATGAGGA AGGTGAAGAT GAAGAAGATG TAGATGTTGA AGAAGAGGAG 
GATGAAGATG AGGAAGATGG TCATACAGTA GAGAGTATCC CAGAAGAAGA AGAAGATGAA 
GAAGAGGAGG AAGAAGGTGA AGAAGAAGAA GGTGAAGAAG AGGAAGATGA AGAAGAAGAA 
GGTGAAGGAG AAGAAGAAGA AGAAGAAGAA GAAGGTGAAG GAGATGATGG CTATGAGCAA 
ATTTCTAGTG ATGAAGATGG AATTGCTGAT TTAGAACGTG AGACTTTTAA ATATCCGAAC 
TTTGATGTTG AATATACTGC TGAGGATTTA GCTTCAGTGC CTCCAATGAC ATATGATCCA 
TATGACAGAG AACTTGTACC ACTTGCATAC TTTAGCTGCC CTTACAAGAC AACTTTTGAA 
ATTGAAGTCA GCAGGATGAA AGACCAAGGT CCTGATAAAG AAAATTCAGG GGCAGTAGAA 
GCATCAGTAA AGTTAGTGGA ACTTTTAGAG TTATACCAGG AAGATAGAGG TCCAAAATGG 
GTCACAGCTT TGGAAGAAGT TCCAGGTTTA ATAGTAAAAG GATTAAGTTA TTTGCAGTTC 
AAAAATGCAC AACAAGACTA CATTGGCCAG CTGGTAGATT GGACCATGCA AGCCTTGAAT 
TTACAAGTAG CTCTCCGTCA GCCCATTGCC TTAAATGTCC GGCAGCTCAA AGCTGGGACT 
AAATTAGTAT CCTCATTAGC AGAATGTGGG CCTCAAGGAG TGATGGGACT TTTACAAGCA 
GGAGTTATCA GTGGATTATT TGAACTGCTA TTTGCTGACC ATGTATCATC TTCTCTTAAG 
CTAAATGCTT TTAAAGCTTT GGACAGTATT ATTAGTATGA CTGAAGGCAT GGAGGATTTT 
TTAAGAGGCA GACAGGACTT ACATGAAAAA AGTGGTTATC AGAAACTTCT GGAGCTCATT 
CTCTTGGATC AGACCGTGAG AGTAGTTACT GCTGGTTCTG CAATTCTCCA GAAATGTCAC 
TTCTATGAGA TTCTGTCTGA AATTAAAAGA CTTGGTGATC AGTTGGCCGA GAGGTCTTCT 
ATTCCTAACC ACAGCGAATC TGAGCAGGAC GCAGACCCTG GACTTGAGAG AACAAATCCT 
GAATATGAAA ATGAAGTGGA AGCTTCTTTG GATATAGTTC ATCTTTTGGA ATCCTCTCTT 
ATAACTGAAG GGGAAATAGA AAAGCTTATT AATCTCTTGG ATGAAATCTT CCACTTAATG 
GAGACTGCAC CTCACACGAT GGTTCAGCCT CCTGTGAAGT CATTCCCGAC AATAGCAAGG 
ATTACTGGAC CTCCAGAGAG AGATGATCCT TATCCTGTCT TATTCAGATA CCTCCACAGC 
CACCACTTCC TAGAATTGGT CACCTTACTG CTGTCAATTC CAGCAATAAG CGCTCACCCT 
GCTGTGCTGC AGGCTATGAG AGATGTTTTG AAGTTTCTTA CACAGTCGCA AAAAGGTCTT 
CTTTTTTTCC TCTCGGAACA TGAGGCGACT AACTTGCTGA TTAGAGCTCT TTGTCACCTT 
CTTGATCAAG AAGAAGAAGA AGGTCTCCAG GCTGATGGTG TTATTGATGA TACATTTGCT 
TTGTGGCTAC AGTATTCAAC TCAGACTTTG CAGTTTATTA CAGAACTATT TTGTCATTTT 
CAGCGTTGCA CAAACAATGA GGAAACTGAC CATTCAGATC TCTTGGGAAC CCTTCATAAT 
CTTTACCAGA TTACTTTTAA TCCTGTGGGA AGAGCAGCTG TTGGTCATGT CTTCAGTCTT 
GAAAAGAATC TCCAGAGTCT TATTACTCTA ATGGAGTACT ATTCCAAAGA GGCTTTAGGG 
GACTCCAAGT CAAAGAAATC GGTAGCTTAT AATTATGCGT GCATACTTGT TTTGTTGGTG 
GTTCAGTCTT CTAGTGATGT CCAGATGCTA GAGCAACATG CAGCGCCTCT CTTGAAGCTT 
TGTAAAGCAG ATGAAAATAA CACGAAATTG CAAGAACTTG GCAAGTGGCT GGAGCCTCTG 
AAAAACCTTA GATTTGAAAT TAATTGTATC CCAAGCCTAA TTGAATATGT TAAACAGAAT 
ATTGATAACT TGATGACCCC AGAAGGAGTT GGTCTACCAA CTGCATTACG TGTCCTCTGT 
AACATAGCGT GCCCACCACC TCCTGTTGAA GGTCAACAGA AAGATCTGAA GTGGAACCTT 
GCAGTTATTC AGCTTTTTTC TGCTGAAGGA ATGGACACCT TTATTCGTGT TCTGCAAAAA 
CTGAACAATA TTCTTACGCA GCCTTGGAGA CTCCATGTCA ACATGGGGAC TACTCTTCAT 
CGAGTTACTA CCATTTCCAT GGCCCGCTGC ACACTCACTC TTCTTAAAAC CATGTTAACA 
GAGCTTCTCA GGGGTGGATC CTTTGAGTTT AAAGACATGC GTGTTCCTTC AGCCCTTGTT 
ACTTTACACA TGCTACTGTG CTCTATCCCT CTCTCAGGTC GTCTGGATAG TGATGAGCAG 
AAGATTCAGA ATGACATCAT TGATATCTTA CTGACTTTTA CGCAGGGAGT TAATGAAAAA 
CTTACAATTT CGGAAGAGAC TTTAGCCAAT AATACTTGGT CTTTAATGTT AAAAGAAGTT 
CTTTCTTCAG TCTTGAGAGT TCCTGAAGGA TTTTTTTCTG GACTCATTCT GCTTTCAGAG 
CTCCTGCCCC TTCCATTGCC CATGCAAACA ACTCAGGTTA TTGAGCCACA TGATATATCA 
GTGGCACTCA ACACTCGCAA ACTGTGGAGT ATGCATCTCC ACGTCCAGGC AAAGCTGCTC 
CAAGAAATAG TTCGTTCTTT CTCTGGCACA ACTTGCCTGC CAATTCAGCA CATGTTGCGG 
CGCATTTGTG TTCAATTATG TGACCTTGCT TCACCAACTG CACTTCTAAT CATGAGAACT 
GTCTTGGATT TGATTGTAGA AGACATGCAA AGCAATTCAG AAGATAAAGA AAAACAGTAT 
ACAAGTCAAA CAACCAGACT TCTGGCTCTT CTTGATGCCC TGGCTTCACA CAAAGCTTGT 
AAATTGGCTA TTTTGCATCT GATAAATGGA ACTATTAAAG GTGATGAACG ATATGCAGAG 
ATTTTCCAGG AATTGTTAGC TCTTCGATCA ACTGGAGAGA GTGTTATTCA TCAACAGTGT 
GTTGAATATG TCATGTCCAT TTTGCAGTCT CTCTGTGATC AGGACATAGC ACTTATTTTG 
CCAAGTTCTT CAGAAGGATC ACTTTCAGAA CTGGAACAGT TGTCCAATTC TTTACCAAAC 
AAGGAATTGA TGGTCTTGAT CTGTGACTGT CTACTGGAAA CAGTAGCCAA TTCTGAGAAT 
AGTTACAACT GCTTACTGAC ATGTGTCAGA ACTATGATGT TTCTTGCAGA ACATGATTAT 
GGATTCTTTC ACTTAAAAAG TTCATTAAGA AAACACAGTA ATGCTTTGCA CCGATTATTG 
AAACGCATTG TTAGTACATT TAGTAAGGAT ACAGGAGAAC TTGCATCTTC TTTTTTGGAA 
TTCATGAGAC AAATACTCAA CTCTGACACA ATGGGATGTT GTGGAGATGA CAGTGGTCTC 
ATGGAAATAG ATGGATCTCA TTCATCAAGA ACATTGAGTC TTAATGCTGC AGAATTAAAA 
CAACTTTTAC AGAGCAAAGA AGAAAGTCCA GAAAGTTTGC TTCTTCAACT GGAGAAGCAT 
ATTTTGGAAC ATTCTAAAGA GGATGACAAC CTGGATTCTT TGCAGGACAA CATAGTTGGT 
CTTAGACAAA TGCTGGAATC TGCAGGTGAT CCTTTACCAC TTAGTGATCA AGATGTGGAA 
CCAGTACTTT CAGCTCCAGA ATCTCTTCAA AATCTCTTTA ATAATAGGAC TGCATATGTG 
CTAGCTGATG TCATGGATGA CCAGCTGAAA TCCATGTGGT TCTCTCCATT CCAGGCTGAA 
GAGATTGAAA CAGATTTAGA TTTGGTAAAA GTTGATCTAA TTGAGCTGTC TGAAAAATGT 
TATAGTGACT TTGATTTGCA AGCCGAATTA GAGCGTTCCT TTTTGTCAGA ACCATCATCT 
CCTGGGAGAA CAAAGACTAC AAAAGGATTT AAACTTGGCA AGCACAAACA TGAGACTTTT 
ATAACATCGA GTGGAAAATC TGAATACATT GAACCTGCCA AGAGAGCACA CATTGTGCCA 
CCTCCCAGAG GAAGAGGCCG GGGAGGATTT GGACAGGGCA TCAGACCCCA TGATATCTTT 
CGTCAGAGGA AACAGAACAC CAGTAGGCCA CCATCTATGC ACGTGGATGA TTTTGTTGCA 
GCTGAAAGCA AAGAAGTGGT CCCACAAGAT GGGTTACCAC CACCAAAAAG GCCACCCAAA 
GTATCACAGA AGATTTCTTC TCGTGGTGGA TTTTCAGGGA ACCGAGGAGG ACGAGGTGCT 
TTTCATAGTC AGAATCGGTT CTTCACTCCA CCTGCTTCAA AAGGAAATTA TAGTCGTCGT 
GAGGGAACAC GAGGTTCTAG CTGGAGTGCG CAGAGTACAC CACGAGGAAC CTATAATGAA 
AGTAGAGGAG GGCAGAGCAA TTTTAGCAGG GGGCCTCTTC CACCCCTTCG GCCACTCAGT 
TCTGCAGGAT ATCGTCCAAG TCCACGCGAC CGTGCTTCCA GAGGTCGGGG TGGACTTGGT 
CCTTCTTGGG CAAGCGCAAA TAGTGGCAGT GGAGGCTCAA GAGGAAAATT TGTTAGTGGA 
GGCAGCGGGA GAGGTCGTCA TGTACGGTCC TTCACACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VIRMA ( XM_023496391.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023352159.1 Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X2, mRNA.

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CloneID	OSa158743
Clone ID Related Accession (Same CDS sequence)	XM_023496391.2 , XM_023496391.1
Accession Version	XM_023496391.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-10
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein virilizer homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823376.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGCAGATG GTGAGAAAGA AGAGCAGTTT AATGGAAGTC CTCCAAGACC ACAGCCCCGA 
GGACCAAGGA CTCCTCCAGG ACCCCCTCCA CCTGATGATG ATGATGATGA TGAACCTCTG 
CCAGTACCAG TTGGTGAGAA AGAAGATGAT GTTCCTCAAC GAGAAGATTA CTTTGAGCCC 
ATTTCACCTG ATCGGACTTC TGTTCATCAA GAAGGGCAGT ATTCAGATGA AGGTGAAGTA 
GAAGAGGATC ATCATGAGGA AGGTGAAGAT GAAGAAGATG TAGATGTTGA AGAAGAGGAG 
GATGAAGATG AGGAAGATGG TCATACAGTA GAGAGTATCC CAGAAGAAGA AGAAGATGAA 
GAAGAGGAGG AAGAAGGTGA AGAAGAAGAA GGTGAAGAAG AGGAAGATGA AGAAGAAGAA 
GGTGAAGGAG AAGAAGAAGA AGAAGAAGAA GAAGGTGAAG GAGATGATGG CTATGAGCAA 
ATTTCTAGTG ATGAAGATGG AATTGCTGAT TTAGAACGTG AGACTTTTAA ATATCCGAAC 
TTTGATGTTG AATATACTGC TGAGGATTTA GCTTCAGTGC CTCCAATGAC ATATGATCCA 
TATGACAGAG AACTTGTACC ACTTGCATAC TTTAGCTGCC CTTACAAGAC AACTTTTGAA 
ATTGAAGTCA GCAGGATGAA AGACCAAGGT CCTGATAAAG AAAATTCAGG GGCAGTAGAA 
GCATCAGTAA AGTTAGTGGA ACTTTTAGAG TTATACCAGG AAGATAGAGG TCCAAAATGG 
GTCACAGCTT TGGAAGAAGT TCCAGGTTTA ATAGTAAAAG GATTAAGTTA TTTGCAGTTC 
AAAAATGCAC AACAAGACTA CATTGGCCAG CTGGTAGATT GGACCATGCA AGCCTTGAAT 
TTACAAGTAG CTCTCCGTCA GCCCATTGCC TTAAATGTCC GGCAGCTCAA AGCTGGGACT 
AAATTAGTAT CCTCATTAGC AGAATGTGGG CCTCAAGGAG TGATGGGACT TTTACAAGCA 
GGAGTTATCA GTGGATTATT TGAACTGCTA TTTGCTGACC ATGTATCATC TTCTCTTAAG 
CTAAATGCTT TTAAAGCTTT GGACAGTATT ATTAGTATGA CTGAAGGCAT GGAGGATTTT 
TTAAGAGGCA GACAGGACTT ACATGAAAAA AGTGGTTATC AGAAACTTCT GGAGCTCATT 
CTCTTGGATC AGACCGTGAG AGTAGTTACT GCTGGTTCTG CAATTCTCCA GAAATGTCAC 
TTCTATGAGA TTCTGTCTGA AATTAAAAGA CTTGGTGATC AGTTGGCCGA GAGGTCTTCT 
ATTCCTAACC ACAGCGAATC TGAGCAGGAC GCAGACCCTG GACTTGAGAG AACAAATCCT 
GAATATGAAA ATGAAGTGGA AGCTTCTTTG GATATAGTTC ATCTTTTGGA ATCCTCTCTT 
ATAACTGAAG GGGAAATAGA AAAGCTTATT AATCTCTTGG ATGAAATCTT CCACTTAATG 
GAGACTGCAC CTCACACGAT GGTTCAGCCT CCTGTGAAGT CATTCCCGAC AATAGCAAGG 
ATTACTGGAC CTCCAGAGAG AGATGATCCT TATCCTGTCT TATTCAGATA CCTCCACAGC 
CACCACTTCC TAGAATTGGT CACCTTACTG CTGTCAATTC CAGCAATAAG CGCTCACCCT 
GCTGTGCTGC AGGCTATGAG AGATGTTTTG AAGTTTCTTA CACAGTCGCA AAAAGGTCTT 
CTTTTTTTCC TCTCGGAACA TGAGGCGACT AACTTGCTGA TTAGAGCTCT TTGTCACCTT 
CTTGATCAAG AAGAAGAAGA AGGTCTCCAG GCTGATGGTG TTATTGATGA TACATTTGCT 
TTGTGGCTAC AGTATTCAAC TCAGACTTTG CAGTTTATTA CAGAACTATT TTGTCATTTT 
CAGCGTTGCA CAAACAATGA GGAAACTGAC CATTCAGATC TCTTGGGAAC CCTTCATAAT 
CTTTACCAGA TTACTTTTAA TCCTGTGGGA AGAGCAGCTG TTGGTCATGT CTTCAGTCTT 
GAAAAGAATC TCCAGAGTCT TATTACTCTA ATGGAGTACT ATTCCAAAGA GGCTTTAGGG 
GACTCCAAGT CAAAGAAATC GGTAGCTTAT AATTATGCGT GCATACTTGT TTTGTTGGTG 
GTTCAGTCTT CTAGTGATGT CCAGATGCTA GAGCAACATG CAGCGCCTCT CTTGAAGCTT 
TGTAAAGCAG ATGAAAATAA CACGAAATTG CAAGAACTTG GCAAGTGGCT GGAGCCTCTG 
AAAAACCTTA GATTTGAAAT TAATTGTATC CCAAGCCTAA TTGAATATGT TAAACAGAAT 
ATTGATAACT TGATGACCCC AGAAGGAGTT GGTCTACCAA CTGCATTACG TGTCCTCTGT 
AACATAGCGT GCCCACCACC TCCTGTTGAA GGTCAACAGA AAGATCTGAA GTGGAACCTT 
GCAGTTATTC AGCTTTTTTC TGCTGAAGGA ATGGACACCT TTATTCGTGT TCTGCAAAAA 
CTGAACAATA TTCTTACGCA GCCTTGGAGA CTCCATGTCA ACATGGGGAC TACTCTTCAT 
CGAGTTACTA CCATTTCCAT GGCCCGCTGC ACACTCACTC TTCTTAAAAC CATGTTAACA 
GAGCTTCTCA GGGGTGGATC CTTTGAGTTT AAAGACATGC GTGTTCCTTC AGCCCTTGTT 
ACTTTACACA TGCTACTGTG CTCTATCCCT CTCTCAGGTC GTCTGGATAG TGATGAGCAG 
AAGATTCAGA ATGACATCAT TGATATCTTA CTGACTTTTA CGCAGGGAGT TAATGAAAAA 
CTTACAATTT CGGAAGAGAC TTTAGCCAAT AATACTTGGT CTTTAATGTT AAAAGAAGTT 
CTTTCTTCAG TCTTGAGAGT TCCTGAAGGA TTTTTTTCTG GACTCATTCT GCTTTCAGAG 
CTCCTGCCCC TTCCATTGCC CATGCAAACA ACTCAGGTTA TTGAGCCACA TGATATATCA 
GTGGCACTCA ACACTCGCAA ACTGTGGAGT ATGCATCTCC ACGTCCAGGC AAAGCTGCTC 
CAAGAAATAG TTCGTTCTTT CTCTGGCACA ACTTGCCTGC CAATTCAGCA CATGTTGCGG 
CGCATTTGTG TTCAATTATG TGACCTTGCT TCACCAACTG CACTTCTAAT CATGAGAACT 
GTCTTGGATT TGATTGTAGA AGACATGCAA AGCAATTCAG AAGATAAAGA AAAACAGTAT 
ACAAGTCAAA CAACCAGACT TCTGGCTCTT CTTGATGCCC TGGCTTCACA CAAAGCTTGT 
AAATTGGCTA TTTTGCATCT GATAAATGGA ACTATTAAAG GTGATGAACG ATATGCAGAG 
ATTTTCCAGG AATTGTTAGC TCTTCGATCA ACTGGAGAGA GTGTTATTCA TCAACAGTGT 
GTTGAATATG TCATGTCCAT TTTGCAGTCT CTCTGTGATC AGGACATAGC ACTTATTTTG 
CCAAGTTCTT CAGAAGGATC ACTTTCAGAA CTGGAACAGT TGTCCAATTC TTTACCAAAC 
AAGGAATTGA TGGTCTTGAT CTGTGACTGT CTACTGGAAA CAGTAGCCAA TTCTGAGAAT 
AGTTACAACT GCTTACTGAC ATGTGTCAGA ACTATGATGT TTCTTGCAGA ACATGATTAT 
GGATTCTTTC ACTTAAAAAG TTCATTAAGA AAACACAGTA ATGCTTTGCA CCGATTATTG 
AAACGCATTG TTAGTACATT TAGTAAGGAT ACAGGAGAAC TTGCATCTTC TTTTTTGGAA 
TTCATGAGAC AAATACTCAA CTCTGACACA ATGGGATGTT GTGGAGATGA CAGTGGTCTC 
ATGGAAATAG ATGGATCTCA TTCATCAAGA ACATTGAGTC TTAATGCTGC AGAATTAAAA 
CAACTTTTAC AGAGCAAAGA AGAAAGTCCA GAAAGTTTGC TTCTTCAACT GGAGAAGCAT 
ATTTTGGAAC ATTCTAAAGA GGATGACAAC CTGGATTCTT TGCAGGACAA CATAGTTGGT 
CTTAGACAAA TGCTGGAATC TGCAGGTGAT CCTTTACCAC TTAGTGATCA AGATGTGGAA 
CCAGTACTTT CAGCTCCAGA ATCTCTTCAA AATCTCTTTA ATAATAGGAC TGCATATGTG 
CTAGCTGATG TCATGGATGA CCAGCTGAAA TCCATGTGGT TCTCTCCATT CCAGGCTGAA 
GAGATTGAAA CAGATTTAGA TTTGGTAAAA GTTGATCTAA TTGAGCTGTC TGAAAAATGT 
TATAGTGACT TTGATTTGCA AGCCGAATTA GAGCGTTCCT TTTTGTCAGA ACCATCATCT 
CCTGGGAGAA CAAAGACTAC AAAAGGATTT AAACTTGGCA AGCACAAACA TGAGACTTTT 
ATAACATCGA GTGGAAAATC TGAATACATT GAACCTGCCA AGAGAGCACA CATTGTGCCA 
CCTCCCAGAG GAAGAGGCCG GGGAGGATTT GGACAGGGCA TCAGACCCCA TGATATCTTT 
CGTCAGAGGA AACAGAACAC CAGTAGGCCA CCATCTATGC ACGTGGATGA TTTTGTTGCA 
GCTGAAAGCA AAGAAGTGGT CCCACAAGAT GGGTTACCAC CACCAAAAAG GCCACCCAAA 
GTATCACAGA AGATTTCTTC TCGTGGTGGA TTTTCAGGGA ACCGAGGAGG ACGAGGTGCT 
TTTCATAGTC AGAATCGGTT CTTCACTCCA CCTGCTTCAA AAGGAAATTA TAGTCGTCGT 
GAGGGAACAC GAGGTTCTAG CTGGAGTGCG CAGAGTACAC CACGAGGAAC CTATAATGAA 
AGTAGAGGAG GGCAGAGCAA TTTTAGCAGG GGGCCTCTTC CACCCCTTCG GCCACTCAGT 
TCTGCAGGAT ATCGTCCAAG TCCACGCGAC CGTGCTTCCA GAGGTCGGGG TGGACTTGGT 
CCTTCTTGGG CAAGCGCAAA TAGTGGCAGT GGAGGCTCAA GAGGAAAATT TGTTAGTGGA 
GGCAGCGGGA GAGGTCGTCA TGTACGGTCC TTCACACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023352159.1
CDS	12..5513
Translation	MAVDSAMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSSLPDNRAYGETSPHTFQLDFFFNNVSKPSAPVFDRLGSLDYDENTSIIFRPNAKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQQQPSVKRTPKHADGEKEEQFNGSPPRPQPRGPRTPPGPPPPDDDDDDEPLPVPVGEKEDDVPQREDYFEPISPDRTSVHQEGQYSDEGEVEEDHHEEGEDEEDVDVEEEEDEDEEDGHTVESIPEEEEDEEEEEEGEEEEGEEEEDEEEEGEGEEEEEEEEGEGDDGYEQISSDEDGIADLERETFKYPNFDVEYTAEDLASVPPMTYDPYDRELVPLAYFSCPYKTTFEIEVSRMKDQGPDKENSGAVEASVKLVELLELYQEDRGPKWVTALEEVPGLIVKGLSYLQFKNAQQDYIGQLVDWTMQALNLQVALRQPIALNVRQLKAGTKLVSSLAECGPQGVMGLLQAGVISGLFELLFADHVSSSLKLNAFKALDSIISMTEGMEDFLRGRQDLHEKSGYQKLLELILLDQTVRVVTAGSAILQKCHFYEILSEIKRLGDQLAERSSIPNHSESEQDADPGLERTNPEYENEVEASLDIVHLLESSLITEGEIEKLINLLDEIFHLMETAPHTMVQPPVKSFPTIARITGPPERDDPYPVLFRYLHSHHFLELVTLLLSIPAISAHPAVLQAMRDVLKFLTQSQKGLLFFLSEHEATNLLIRALCHLLDQEEEEGLQADGVIDDTFALWLQYSTQTLQFITELFCHFQRCTNNEETDHSDLLGTLHNLYQITFNPVGRAAVGHVFSLEKNLQSLITLMEYYSKEALGDSKSKKSVAYNYACILVLLVVQSSSDVQMLEQHAAPLLKLCKADENNTKLQELGKWLEPLKNLRFEINCIPSLIEYVKQNIDNLMTPEGVGLPTALRVLCNIACPPPPVEGQQKDLKWNLAVIQLFSAEGMDTFIRVLQKLNNILTQPWRLHVNMGTTLHRVTTISMARCTLTLLKTMLTELLRGGSFEFKDMRVPSALVTLHMLLCSIPLSGRLDSDEQKIQNDIIDILLTFTQGVNEKLTISEETLANNTWSLMLKEVLSSVLRVPEGFFSGLILLSELLPLPLPMQTTQVIEPHDISVALNTRKLWSMHLHVQAKLLQEIVRSFSGTTCLPIQHMLRRICVQLCDLASPTALLIMRTVLDLIVEDMQSNSEDKEKQYTSQTTRLLALLDALASHKACKLAILHLINGTIKGDERYAEIFQELLALRSTGESVIHQQCVEYVMSILQSLCDQDIALILPSSSEGSLSELEQLSNSLPNKELMVLICDCLLETVANSENSYNCLLTCVRTMMFLAEHDYGFFHLKSSLRKHSNALHRLLKRIVSTFSKDTGELASSFLEFMRQILNSDTMGCCGDDSGLMEIDGSHSSRTLSLNAAELKQLLQSKEESPESLLLQLEKHILEHSKEDDNLDSLQDNIVGLRQMLESAGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFSPFQAEEIETDLDLVKVDLIELSEKCYSDFDLQAELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHIVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGLPPPKRPPKVSQKISSRGGFSGNRGGRGAFHSQNRFFTPPASKGNYSRREGTRGSSWSAQSTPRGTYNESRGGQSNFSRGPLPPLRPLSSAGYRPSPRDRASRGRGGLGPSWASANSGSGGSRGKFVSGGSGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_023496391.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023496391.1

ORF Insert Sequence:

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGCAGATG GTGAGAAAGA AGAGCAGTTT AATGGAAGTC CTCCAAGACC ACAGCCCCGA 
GGACCAAGGA CTCCTCCAGG ACCCCCTCCA CCTGATGATG ATGATGATGA TGAACCTCTG 
CCAGTACCAG TTGGTGAGAA AGAAGATGAT GTTCCTCAAC GAGAAGATTA CTTTGAGCCC 
ATTTCACCTG ATCGGACTTC TGTTCATCAA GAAGGGCAGT ATTCAGATGA AGGTGAAGTA 
GAAGAGGATC ATCATGAGGA AGGTGAAGAT GAAGAAGATG TAGATGTTGA AGAAGAGGAG 
GATGAAGATG AGGAAGATGG TCATACAGTA GAGAGTATCC CAGAAGAAGA AGAAGATGAA 
GAAGAGGAGG AAGAAGGTGA AGAAGAAGAA GGTGAAGAAG AGGAAGATGA AGAAGAAGAA 
GGTGAAGGAG AAGAAGAAGA AGAAGAAGAA GAAGGTGAAG GAGATGATGG CTATGAGCAA 
ATTTCTAGTG ATGAAGATGG AATTGCTGAT TTAGAACGTG AGACTTTTAA ATATCCGAAC 
TTTGATGTTG AATATACTGC TGAGGATTTA GCTTCAGTGC CTCCAATGAC ATATGATCCA 
TATGACAGAG AACTTGTACC ACTTGCATAC TTTAGCTGCC CTTACAAGAC AACTTTTGAA 
ATTGAAGTCA GCAGGATGAA AGACCAAGGT CCTGATAAAG AAAATTCAGG GGCAGTAGAA 
GCATCAGTAA AGTTAGTGGA ACTTTTAGAG TTATACCAGG AAGATAGAGG TCCAAAATGG 
GTCACAGCTT TGGAAGAAGT TCCAGGTTTA ATAGTAAAAG GATTAAGTTA TTTGCAGTTC 
AAAAATGCAC AACAAGACTA CATTGGCCAG CTGGTAGATT GGACCATGCA AGCCTTGAAT 
TTACAAGTAG CTCTCCGTCA GCCCATTGCC TTAAATGTCC GGCAGCTCAA AGCTGGGACT 
AAATTAGTAT CCTCATTAGC AGAATGTGGG CCTCAAGGAG TGATGGGACT TTTACAAGCA 
GGAGTTATCA GTGGATTATT TGAACTGCTA TTTGCTGACC ATGTATCATC TTCTCTTAAG 
CTAAATGCTT TTAAAGCTTT GGACAGTATT ATTAGTATGA CTGAAGGCAT GGAGGATTTT 
TTAAGAGGCA GACAGGACTT ACATGAAAAA AGTGGTTATC AGAAACTTCT GGAGCTCATT 
CTCTTGGATC AGACCGTGAG AGTAGTTACT GCTGGTTCTG CAATTCTCCA GAAATGTCAC 
TTCTATGAGA TTCTGTCTGA AATTAAAAGA CTTGGTGATC AGTTGGCCGA GAGGTCTTCT 
ATTCCTAACC ACAGCGAATC TGAGCAGGAC GCAGACCCTG GACTTGAGAG AACAAATCCT 
GAATATGAAA ATGAAGTGGA AGCTTCTTTG GATATAGTTC ATCTTTTGGA ATCCTCTCTT 
ATAACTGAAG GGGAAATAGA AAAGCTTATT AATCTCTTGG ATGAAATCTT CCACTTAATG 
GAGACTGCAC CTCACACGAT GGTTCAGCCT CCTGTGAAGT CATTCCCGAC AATAGCAAGG 
ATTACTGGAC CTCCAGAGAG AGATGATCCT TATCCTGTCT TATTCAGATA CCTCCACAGC 
CACCACTTCC TAGAATTGGT CACCTTACTG CTGTCAATTC CAGCAATAAG CGCTCACCCT 
GCTGTGCTGC AGGCTATGAG AGATGTTTTG AAGTTTCTTA CACAGTCGCA AAAAGGTCTT 
CTTTTTTTCC TCTCGGAACA TGAGGCGACT AACTTGCTGA TTAGAGCTCT TTGTCACCTT 
CTTGATCAAG AAGAAGAAGA AGGTCTCCAG GCTGATGGTG TTATTGATGA TACATTTGCT 
TTGTGGCTAC AGTATTCAAC TCAGACTTTG CAGTTTATTA CAGAACTATT TTGTCATTTT 
CAGCGTTGCA CAAACAATGA GGAAACTGAC CATTCAGATC TCTTGGGAAC CCTTCATAAT 
CTTTACCAGA TTACTTTTAA TCCTGTGGGA AGAGCAGCTG TTGGTCATGT CTTCAGTCTT 
GAAAAGAATC TCCAGAGTCT TATTACTCTA ATGGAGTACT ATTCCAAAGA GGCTTTAGGG 
GACTCCAAGT CAAAGAAATC GGTAGCTTAT AATTATGCGT GCATACTTGT TTTGTTGGTG 
GTTCAGTCTT CTAGTGATGT CCAGATGCTA GAGCAACATG CAGCGCCTCT CTTGAAGCTT 
TGTAAAGCAG ATGAAAATAA CACGAAATTG CAAGAACTTG GCAAGTGGCT GGAGCCTCTG 
AAAAACCTTA GATTTGAAAT TAATTGTATC CCAAGCCTAA TTGAATATGT TAAACAGAAT 
ATTGATAACT TGATGACCCC AGAAGGAGTT GGTCTACCAA CTGCATTACG TGTCCTCTGT 
AACATAGCGT GCCCACCACC TCCTGTTGAA GGTCAACAGA AAGATCTGAA GTGGAACCTT 
GCAGTTATTC AGCTTTTTTC TGCTGAAGGA ATGGACACCT TTATTCGTGT TCTGCAAAAA 
CTGAACAATA TTCTTACGCA GCCTTGGAGA CTCCATGTCA ACATGGGGAC TACTCTTCAT 
CGAGTTACTA CCATTTCCAT GGCCCGCTGC ACACTCACTC TTCTTAAAAC CATGTTAACA 
GAGCTTCTCA GGGGTGGATC CTTTGAGTTT AAAGACATGC GTGTTCCTTC AGCCCTTGTT 
ACTTTACACA TGCTACTGTG CTCTATCCCT CTCTCAGGTC GTCTGGATAG TGATGAGCAG 
AAGATTCAGA ATGACATCAT TGATATCTTA CTGACTTTTA CGCAGGGAGT TAATGAAAAA 
CTTACAATTT CGGAAGAGAC TTTAGCCAAT AATACTTGGT CTTTAATGTT AAAAGAAGTT 
CTTTCTTCAG TCTTGAGAGT TCCTGAAGGA TTTTTTTCTG GACTCATTCT GCTTTCAGAG 
CTCCTGCCCC TTCCATTGCC CATGCAAACA ACTCAGGTTA TTGAGCCACA TGATATATCA 
GTGGCACTCA ACACTCGCAA ACTGTGGAGT ATGCATCTCC ACGTCCAGGC AAAGCTGCTC 
CAAGAAATAG TTCGTTCTTT CTCTGGCACA ACTTGCCTGC CAATTCAGCA CATGTTGCGG 
CGCATTTGTG TTCAATTATG TGACCTTGCT TCACCAACTG CACTTCTAAT CATGAGAACT 
GTCTTGGATT TGATTGTAGA AGACATGCAA AGCAATTCAG AAGATAAAGA AAAACAGTAT 
ACAAGTCAAA CAACCAGACT TCTGGCTCTT CTTGATGCCC TGGCTTCACA CAAAGCTTGT 
AAATTGGCTA TTTTGCATCT GATAAATGGA ACTATTAAAG GTGATGAACG ATATGCAGAG 
ATTTTCCAGG AATTGTTAGC TCTTCGATCA ACTGGAGAGA GTGTTATTCA TCAACAGTGT 
GTTGAATATG TCATGTCCAT TTTGCAGTCT CTCTGTGATC AGGACATAGC ACTTATTTTG 
CCAAGTTCTT CAGAAGGATC ACTTTCAGAA CTGGAACAGT TGTCCAATTC TTTACCAAAC 
AAGGAATTGA TGGTCTTGAT CTGTGACTGT CTACTGGAAA CAGTAGCCAA TTCTGAGAAT 
AGTTACAACT GCTTACTGAC ATGTGTCAGA ACTATGATGT TTCTTGCAGA ACATGATTAT 
GGATTCTTTC ACTTAAAAAG TTCATTAAGA AAACACAGTA ATGCTTTGCA CCGATTATTG 
AAACGCATTG TTAGTACATT TAGTAAGGAT ACAGGAGAAC TTGCATCTTC TTTTTTGGAA 
TTCATGAGAC AAATACTCAA CTCTGACACA ATGGGATGTT GTGGAGATGA CAGTGGTCTC 
ATGGAAATAG ATGGATCTCA TTCATCAAGA ACATTGAGTC TTAATGCTGC AGAATTAAAA 
CAACTTTTAC AGAGCAAAGA AGAAAGTCCA GAAAGTTTGC TTCTTCAACT GGAGAAGCAT 
ATTTTGGAAC ATTCTAAAGA GGATGACAAC CTGGATTCTT TGCAGGACAA CATAGTTGGT 
CTTAGACAAA TGCTGGAATC TGCAGGTGAT CCTTTACCAC TTAGTGATCA AGATGTGGAA 
CCAGTACTTT CAGCTCCAGA ATCTCTTCAA AATCTCTTTA ATAATAGGAC TGCATATGTG 
CTAGCTGATG TCATGGATGA CCAGCTGAAA TCCATGTGGT TCTCTCCATT CCAGGCTGAA 
GAGATTGAAA CAGATTTAGA TTTGGTAAAA GTTGATCTAA TTGAGCTGTC TGAAAAATGT 
TATAGTGACT TTGATTTGCA AGCCGAATTA GAGCGTTCCT TTTTGTCAGA ACCATCATCT 
CCTGGGAGAA CAAAGACTAC AAAAGGATTT AAACTTGGCA AGCACAAACA TGAGACTTTT 
ATAACATCGA GTGGAAAATC TGAATACATT GAACCTGCCA AGAGAGCACA CATTGTGCCA 
CCTCCCAGAG GAAGAGGCCG GGGAGGATTT GGACAGGGCA TCAGACCCCA TGATATCTTT 
CGTCAGAGGA AACAGAACAC CAGTAGGCCA CCATCTATGC ACGTGGATGA TTTTGTTGCA 
GCTGAAAGCA AAGAAGTGGT CCCACAAGAT GGGTTACCAC CACCAAAAAG GCCACCCAAA 
GTATCACAGA AGATTTCTTC TCGTGGTGGA TTTTCAGGGA ACCGAGGAGG ACGAGGTGCT 
TTTCATAGTC AGAATCGGTT CTTCACTCCA CCTGCTTCAA AAGGAAATTA TAGTCGTCGT 
GAGGGAACAC GAGGTTCTAG CTGGAGTGCG CAGAGTACAC CACGAGGAAC CTATAATGAA 
AGTAGAGGAG GGCAGAGCAA TTTTAGCAGG GGGCCTCTTC CACCCCTTCG GCCACTCAGT 
TCTGCAGGAT ATCGTCCAAG TCCACGCGAC CGTGCTTCCA GAGGTCGGGG TGGACTTGGT 
CCTTCTTGGG CAAGCGCAAA TAGTGGCAGT GGAGGCTCAA GAGGAAAATT TGTTAGTGGA 
GGCAGCGGGA GAGGTCGTCA TGTACGGTCC TTCACACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VIRMA ( XM_023496392.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023352160.1 Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X3, mRNA.

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CloneID	OSa158744
Clone ID Related Accession (Same CDS sequence)	XM_023496392.1 , XM_023496392.2
Accession Version	XM_023496392.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-10
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein virilizer homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823376.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGAGTTCCC ATATAGATGT GGTTCGTTTT CCGTGTGTGG TTTATATCAA TGAAGTCCGT 
GTCATTCCAC CAGGAGTAAG AGCTCATAGT AGTCTGCCTG ATAATAGAGC ATATGGGGAA 
ACCTCTCCGC ATACATTTCA GCTAGACTTT TTTTTCAACA ATGTAAGCAA ACCAAGTGCT 
CCCGTTTTTG ATAGGCTGGG AAGTCTGGAC TATGATGAAA ACACCTCTAT CATCTTTAGA 
CCTAATGCAA AGGTAAATAC TGATGGCTTG GTTCTAAGAG GTTGGTACAA CTGTCTGACA 
CTAGCAATAT ATGGATCCGT TGATAGAGTG ATAAGTCATG ATAGAGACTC TCCACCACCA 
CCACCTCCAC CTCCACCTCC TCCACAGCAG CAGCCAAGTG TAAAAAGAAC TCCAAAACAT 
GCAGATGGTG AGAAAGAAGA GCAGTTTAAT GGAAGTCCTC CAAGACCACA GCCCCGAGGA 
CCAAGGACTC CTCCAGGACC CCCTCCACCT GATGATGATG ATGATGATGA ACCTCTGCCA 
GTACCAGTAG TTGGTGAGAA AGAAGATGAT GTTCCTCAAC GAGAAGATTA CTTTGAGCCC 
ATTTCACCTG ATCGGACTTC TGTTCATCAA GAAGGGCAGT ATTCAGATGA AGGTGAAGTA 
GAAGAGGATC ATCATGAGGA AGGTGAAGAT GAAGAAGATG TAGATGTTGA AGAAGAGGAG 
GATGAAGATG AGGAAGATGG TCATACAGTA GAGAGTATCC CAGAAGAAGA AGAAGATGAA 
GAAGAGGAGG AAGAAGGTGA AGAAGAAGAA GGTGAAGAAG AGGAAGATGA AGAAGAAGAA 
GGTGAAGGAG AAGAAGAAGA AGAAGAAGAA GAAGGTGAAG GAGATGATGG CTATGAGCAA 
ATTTCTAGTG ATGAAGATGG AATTGCTGAT TTAGAACGTG AGACTTTTAA ATATCCGAAC 
TTTGATGTTG AATATACTGC TGAGGATTTA GCTTCAGTGC CTCCAATGAC ATATGATCCA 
TATGACAGAG AACTTGTACC ACTTGCATAC TTTAGCTGCC CTTACAAGAC AACTTTTGAA 
ATTGAAGTCA GCAGGATGAA AGACCAAGGT CCTGATAAAG AAAATTCAGG GGCAGTAGAA 
GCATCAGTAA AGTTAGTGGA ACTTTTAGAG TTATACCAGG AAGATAGAGG TCCAAAATGG 
GTCACAGCTT TGGAAGAAGT TCCAGGTTTA ATAGTAAAAG GATTAAGTTA TTTGCAGTTC 
AAAAATGCAC AACAAGACTA CATTGGCCAG CTGGTAGATT GGACCATGCA AGCCTTGAAT 
TTACAAGTAG CTCTCCGTCA GCCCATTGCC TTAAATGTCC GGCAGCTCAA AGCTGGGACT 
AAATTAGTAT CCTCATTAGC AGAATGTGGG CCTCAAGGAG TGATGGGACT TTTACAAGCA 
GGAGTTATCA GTGGATTATT TGAACTGCTA TTTGCTGACC ATGTATCATC TTCTCTTAAG 
CTAAATGCTT TTAAAGCTTT GGACAGTATT ATTAGTATGA CTGAAGGCAT GGAGGATTTT 
TTAAGAGGCA GACAGGACTT ACATGAAAAA AGTGGTTATC AGAAACTTCT GGAGCTCATT 
CTCTTGGATC AGACCGTGAG AGTAGTTACT GCTGGTTCTG CAATTCTCCA GAAATGTCAC 
TTCTATGAGA TTCTGTCTGA AATTAAAAGA CTTGGTGATC AGTTGGCCGA GAGGTCTTCT 
ATTCCTAACC ACAGCGAATC TGAGCAGGAC GCAGACCCTG GACTTGAGAG AACAAATCCT 
GAATATGAAA ATGAAGTGGA AGCTTCTTTG GATATAGTTC ATCTTTTGGA ATCCTCTCTT 
ATAACTGAAG GGGAAATAGA AAAGCTTATT AATCTCTTGG ATGAAATCTT CCACTTAATG 
GAGACTGCAC CTCACACGAT GGTTCAGCCT CCTGTGAAGT CATTCCCGAC AATAGCAAGG 
ATTACTGGAC CTCCAGAGAG AGATGATCCT TATCCTGTCT TATTCAGATA CCTCCACAGC 
CACCACTTCC TAGAATTGGT CACCTTACTG CTGTCAATTC CAGCAATAAG CGCTCACCCT 
GCTGTGCTGC AGGCTATGAG AGATGTTTTG AAGTTTCTTA CACAGTCGCA AAAAGGTCTT 
CTTTTTTTCC TCTCGGAACA TGAGGCGACT AACTTGCTGA TTAGAGCTCT TTGTCACCTT 
CTTGATCAAG AAGAAGAAGA AGGTCTCCAG GCTGATGGTG TTATTGATGA TACATTTGCT 
TTGTGGCTAC AGTATTCAAC TCAGACTTTG CAGTTTATTA CAGAACTATT TTGTCATTTT 
CAGCGTTGCA CAAACAATGA GGAAACTGAC CATTCAGATC TCTTGGGAAC CCTTCATAAT 
CTTTACCAGA TTACTTTTAA TCCTGTGGGA AGAGCAGCTG TTGGTCATGT CTTCAGTCTT 
GAAAAGAATC TCCAGAGTCT TATTACTCTA ATGGAGTACT ATTCCAAAGA GGCTTTAGGG 
GACTCCAAGT CAAAGAAATC GGTAGCTTAT AATTATGCGT GCATACTTGT TTTGTTGGTG 
GTTCAGTCTT CTAGTGATGT CCAGATGCTA GAGCAACATG CAGCGCCTCT CTTGAAGCTT 
TGTAAAGCAG ATGAAAATAA CACGAAATTG CAAGAACTTG GCAAGTGGCT GGAGCCTCTG 
AAAAACCTTA GATTTGAAAT TAATTGTATC CCAAGCCTAA TTGAATATGT TAAACAGAAT 
ATTGATAACT TGATGACCCC AGAAGGAGTT GGTCTACCAA CTGCATTACG TGTCCTCTGT 
AACATAGCGT GCCCACCACC TCCTGTTGAA GGTCAACAGA AAGATCTGAA GTGGAACCTT 
GCAGTTATTC AGCTTTTTTC TGCTGAAGGA ATGGACACCT TTATTCGTGT TCTGCAAAAA 
CTGAACAATA TTCTTACGCA GCCTTGGAGA CTCCATGTCA ACATGGGGAC TACTCTTCAT 
CGAGTTACTA CCATTTCCAT GGCCCGCTGC ACACTCACTC TTCTTAAAAC CATGTTAACA 
GAGCTTCTCA GGGGTGGATC CTTTGAGTTT AAAGACATGC GTGTTCCTTC AGCCCTTGTT 
ACTTTACACA TGCTACTGTG CTCTATCCCT CTCTCAGGTC GTCTGGATAG TGATGAGCAG 
AAGATTCAGA ATGACATCAT TGATATCTTA CTGACTTTTA CGCAGGGAGT TAATGAAAAA 
CTTACAATTT CGGAAGAGAC TTTAGCCAAT AATACTTGGT CTTTAATGTT AAAAGAAGTT 
CTTTCTTCAG TCTTGAGAGT TCCTGAAGGA TTTTTTTCTG GACTCATTCT GCTTTCAGAG 
CTCCTGCCCC TTCCATTGCC CATGCAAACA ACTCAGGTTA TTGAGCCACA TGATATATCA 
GTGGCACTCA ACACTCGCAA ACTGTGGAGT ATGCATCTCC ACGTCCAGGC AAAGCTGCTC 
CAAGAAATAG TTCGTTCTTT CTCTGGCACA ACTTGCCTGC CAATTCAGCA CATGTTGCGG 
CGCATTTGTG TTCAATTATG TGACCTTGCT TCACCAACTG CACTTCTAAT CATGAGAACT 
GTCTTGGATT TGATTGTAGA AGACATGCAA AGCAATTCAG AAGATAAAGA AAAACAGTAT 
ACAAGTCAAA CAACCAGACT TCTGGCTCTT CTTGATGCCC TGGCTTCACA CAAAGCTTGT 
AAATTGGCTA TTTTGCATCT GATAAATGGA ACTATTAAAG GTGATGAACG ATATGCAGAG 
ATTTTCCAGG AATTGTTAGC TCTTCGATCA ACTGGAGAGA GTGTTATTCA TCAACAGTGT 
GTTGAATATG TCATGTCCAT TTTGCAGTCT CTCTGTGATC AGGACATAGC ACTTATTTTG 
CCAAGTTCTT CAGAAGGATC ACTTTCAGAA CTGGAACAGT TGTCCAATTC TTTACCAAAC 
AAGGAATTGA TGGTCTTGAT CTGTGACTGT CTACTGGAAA CAGTAGCCAA TTCTGAGAAT 
AGTTACAACT GCTTACTGAC ATGTGTCAGA ACTATGATGT TTCTTGCAGA ACATGATTAT 
GGATTCTTTC ACTTAAAAAG TTCATTAAGA AAACACAGTA ATGCTTTGCA CCGATTATTG 
AAACGCATTG TTAGTACATT TAGTAAGGAT ACAGGAGAAC TTGCATCTTC TTTTTTGGAA 
TTCATGAGAC AAATACTCAA CTCTGACACA ATGGGATGTT GTGGAGATGA CAGTGGTCTC 
ATGGAAATAG ATGGATCTCA TTCATCAAGA ACATTGAGTC TTAATGCTGC AGAATTAAAA 
CAACTTTTAC AGAGCAAAGA AGAAAGTCCA GAAAGTTTGC TTCTTCAACT GGAGAAGCAT 
ATTTTGGAAC ATTCTAAAGA GGATGACAAC CTGGATTCTT TGCAGGACAA CATAGTTGGT 
CTTAGACAAA TGCTGGAATC TGCAGGTGAT CCTTTACCAC TTAGTGATCA AGATGTGGAA 
CCAGTACTTT CAGCTCCAGA ATCTCTTCAA AATCTCTTTA ATAATAGGAC TGCATATGTG 
CTAGCTGATG TCATGGATGA CCAGCTGAAA TCCATGTGGT TCTCTCCATT CCAGGCTGAA 
GAGATTGAAA CAGATTTAGA TTTGGTAAAA GTTGATCTAA TTGAGCTGTC TGAAAAATGT 
TATAGTGACT TTGATTTGCA AGCCGAATTA GAGCGTTCCT TTTTGTCAGA ACCATCATCT 
CCTGGGAGAA CAAAGACTAC AAAAGGATTT AAACTTGGCA AGCACAAACA TGAGACTTTT 
ATAACATCGA GTGGAAAATC TGAATACATT GAACCTGCCA AGAGAGCACA CATTGTGCCA 
CCTCCCAGAG GAAGAGGCCG GGGAGGATTT GGACAGGGCA TCAGACCCCA TGATATCTTT 
CGTCAGAGGA AACAGAACAC CAGTAGGCCA CCATCTATGC ACGTGGATGA TTTTGTTGCA 
GCTGAAAGCA AAGAAGTGGT CCCACAAGAT GGGTTACCAC CACCAAAAAG GCCACCCAAA 
GTATCACAGA AGATTTCTTC TCGTGGTGGA TTTTCAGGGA ACCGAGGAGG ACGAGGTGCT 
TTTCATAGTC AGAATCGGTT CTTCACTCCA CCTGCTTCAA AAGGAAATTA TAGTCGTCGT 
GAGGGAACAC GAGGTTCTAG CTGGAGTGCG CAGAGTACAC CACGAGGAAC CTATAATGAA 
AGTAGAGGAG GGCAGAGCAA TTTTAGCAGG GGGCCTCTTC CACCCCTTCG GCCACTCAGT 
TCTGCAGGAT ATCGTCCAAG TCCACGCGAC CGTGCTTCCA GAGGTCGGGG TGGACTTGGT 
CCTTCTTGGG CAAGCGCAAA TAGTGGCAGT GGAGGCTCAA GAGGAAAATT TGTTAGTGGA 
GGCAGCGGGA GAGGTCGTCA TGTACGGTCC TTCACACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023352160.1
CDS	14..5515
Translation	MAVDSAMELLFLDTFKHPSAESSHIDVVRFPCVVYINEVRVIPPGVRAHSSLPDNRAYGETSPHTFQLDFFFNNVSKPSAPVFDRLGSLDYDENTSIIFRPNAKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQQQPSVKRTPKHADGEKEEQFNGSPPRPQPRGPRTPPGPPPPDDDDDDEPLPVPVVGEKEDDVPQREDYFEPISPDRTSVHQEGQYSDEGEVEEDHHEEGEDEEDVDVEEEEDEDEEDGHTVESIPEEEEDEEEEEEGEEEEGEEEEDEEEEGEGEEEEEEEEGEGDDGYEQISSDEDGIADLERETFKYPNFDVEYTAEDLASVPPMTYDPYDRELVPLAYFSCPYKTTFEIEVSRMKDQGPDKENSGAVEASVKLVELLELYQEDRGPKWVTALEEVPGLIVKGLSYLQFKNAQQDYIGQLVDWTMQALNLQVALRQPIALNVRQLKAGTKLVSSLAECGPQGVMGLLQAGVISGLFELLFADHVSSSLKLNAFKALDSIISMTEGMEDFLRGRQDLHEKSGYQKLLELILLDQTVRVVTAGSAILQKCHFYEILSEIKRLGDQLAERSSIPNHSESEQDADPGLERTNPEYENEVEASLDIVHLLESSLITEGEIEKLINLLDEIFHLMETAPHTMVQPPVKSFPTIARITGPPERDDPYPVLFRYLHSHHFLELVTLLLSIPAISAHPAVLQAMRDVLKFLTQSQKGLLFFLSEHEATNLLIRALCHLLDQEEEEGLQADGVIDDTFALWLQYSTQTLQFITELFCHFQRCTNNEETDHSDLLGTLHNLYQITFNPVGRAAVGHVFSLEKNLQSLITLMEYYSKEALGDSKSKKSVAYNYACILVLLVVQSSSDVQMLEQHAAPLLKLCKADENNTKLQELGKWLEPLKNLRFEINCIPSLIEYVKQNIDNLMTPEGVGLPTALRVLCNIACPPPPVEGQQKDLKWNLAVIQLFSAEGMDTFIRVLQKLNNILTQPWRLHVNMGTTLHRVTTISMARCTLTLLKTMLTELLRGGSFEFKDMRVPSALVTLHMLLCSIPLSGRLDSDEQKIQNDIIDILLTFTQGVNEKLTISEETLANNTWSLMLKEVLSSVLRVPEGFFSGLILLSELLPLPLPMQTTQVIEPHDISVALNTRKLWSMHLHVQAKLLQEIVRSFSGTTCLPIQHMLRRICVQLCDLASPTALLIMRTVLDLIVEDMQSNSEDKEKQYTSQTTRLLALLDALASHKACKLAILHLINGTIKGDERYAEIFQELLALRSTGESVIHQQCVEYVMSILQSLCDQDIALILPSSSEGSLSELEQLSNSLPNKELMVLICDCLLETVANSENSYNCLLTCVRTMMFLAEHDYGFFHLKSSLRKHSNALHRLLKRIVSTFSKDTGELASSFLEFMRQILNSDTMGCCGDDSGLMEIDGSHSSRTLSLNAAELKQLLQSKEESPESLLLQLEKHILEHSKEDDNLDSLQDNIVGLRQMLESAGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFSPFQAEEIETDLDLVKVDLIELSEKCYSDFDLQAELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHIVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGLPPPKRPPKVSQKISSRGGFSGNRGGRGAFHSQNRFFTPPASKGNYSRREGTRGSSWSAQSTPRGTYNESRGGQSNFSRGPLPPLRPLSSAGYRPSPRDRASRGRGGLGPSWASANSGSGGSRGKFVSGGSGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_023496392.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023496392.1

ORF Insert Sequence:

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGAGTTCCC ATATAGATGT GGTTCGTTTT CCGTGTGTGG TTTATATCAA TGAAGTCCGT 
GTCATTCCAC CAGGAGTAAG AGCTCATAGT AGTCTGCCTG ATAATAGAGC ATATGGGGAA 
ACCTCTCCGC ATACATTTCA GCTAGACTTT TTTTTCAACA ATGTAAGCAA ACCAAGTGCT 
CCCGTTTTTG ATAGGCTGGG AAGTCTGGAC TATGATGAAA ACACCTCTAT CATCTTTAGA 
CCTAATGCAA AGGTAAATAC TGATGGCTTG GTTCTAAGAG GTTGGTACAA CTGTCTGACA 
CTAGCAATAT ATGGATCCGT TGATAGAGTG ATAAGTCATG ATAGAGACTC TCCACCACCA 
CCACCTCCAC CTCCACCTCC TCCACAGCAG CAGCCAAGTG TAAAAAGAAC TCCAAAACAT 
GCAGATGGTG AGAAAGAAGA GCAGTTTAAT GGAAGTCCTC CAAGACCACA GCCCCGAGGA 
CCAAGGACTC CTCCAGGACC CCCTCCACCT GATGATGATG ATGATGATGA ACCTCTGCCA 
GTACCAGTAG TTGGTGAGAA AGAAGATGAT GTTCCTCAAC GAGAAGATTA CTTTGAGCCC 
ATTTCACCTG ATCGGACTTC TGTTCATCAA GAAGGGCAGT ATTCAGATGA AGGTGAAGTA 
GAAGAGGATC ATCATGAGGA AGGTGAAGAT GAAGAAGATG TAGATGTTGA AGAAGAGGAG 
GATGAAGATG AGGAAGATGG TCATACAGTA GAGAGTATCC CAGAAGAAGA AGAAGATGAA 
GAAGAGGAGG AAGAAGGTGA AGAAGAAGAA GGTGAAGAAG AGGAAGATGA AGAAGAAGAA 
GGTGAAGGAG AAGAAGAAGA AGAAGAAGAA GAAGGTGAAG GAGATGATGG CTATGAGCAA 
ATTTCTAGTG ATGAAGATGG AATTGCTGAT TTAGAACGTG AGACTTTTAA ATATCCGAAC 
TTTGATGTTG AATATACTGC TGAGGATTTA GCTTCAGTGC CTCCAATGAC ATATGATCCA 
TATGACAGAG AACTTGTACC ACTTGCATAC TTTAGCTGCC CTTACAAGAC AACTTTTGAA 
ATTGAAGTCA GCAGGATGAA AGACCAAGGT CCTGATAAAG AAAATTCAGG GGCAGTAGAA 
GCATCAGTAA AGTTAGTGGA ACTTTTAGAG TTATACCAGG AAGATAGAGG TCCAAAATGG 
GTCACAGCTT TGGAAGAAGT TCCAGGTTTA ATAGTAAAAG GATTAAGTTA TTTGCAGTTC 
AAAAATGCAC AACAAGACTA CATTGGCCAG CTGGTAGATT GGACCATGCA AGCCTTGAAT 
TTACAAGTAG CTCTCCGTCA GCCCATTGCC TTAAATGTCC GGCAGCTCAA AGCTGGGACT 
AAATTAGTAT CCTCATTAGC AGAATGTGGG CCTCAAGGAG TGATGGGACT TTTACAAGCA 
GGAGTTATCA GTGGATTATT TGAACTGCTA TTTGCTGACC ATGTATCATC TTCTCTTAAG 
CTAAATGCTT TTAAAGCTTT GGACAGTATT ATTAGTATGA CTGAAGGCAT GGAGGATTTT 
TTAAGAGGCA GACAGGACTT ACATGAAAAA AGTGGTTATC AGAAACTTCT GGAGCTCATT 
CTCTTGGATC AGACCGTGAG AGTAGTTACT GCTGGTTCTG CAATTCTCCA GAAATGTCAC 
TTCTATGAGA TTCTGTCTGA AATTAAAAGA CTTGGTGATC AGTTGGCCGA GAGGTCTTCT 
ATTCCTAACC ACAGCGAATC TGAGCAGGAC GCAGACCCTG GACTTGAGAG AACAAATCCT 
GAATATGAAA ATGAAGTGGA AGCTTCTTTG GATATAGTTC ATCTTTTGGA ATCCTCTCTT 
ATAACTGAAG GGGAAATAGA AAAGCTTATT AATCTCTTGG ATGAAATCTT CCACTTAATG 
GAGACTGCAC CTCACACGAT GGTTCAGCCT CCTGTGAAGT CATTCCCGAC AATAGCAAGG 
ATTACTGGAC CTCCAGAGAG AGATGATCCT TATCCTGTCT TATTCAGATA CCTCCACAGC 
CACCACTTCC TAGAATTGGT CACCTTACTG CTGTCAATTC CAGCAATAAG CGCTCACCCT 
GCTGTGCTGC AGGCTATGAG AGATGTTTTG AAGTTTCTTA CACAGTCGCA AAAAGGTCTT 
CTTTTTTTCC TCTCGGAACA TGAGGCGACT AACTTGCTGA TTAGAGCTCT TTGTCACCTT 
CTTGATCAAG AAGAAGAAGA AGGTCTCCAG GCTGATGGTG TTATTGATGA TACATTTGCT 
TTGTGGCTAC AGTATTCAAC TCAGACTTTG CAGTTTATTA CAGAACTATT TTGTCATTTT 
CAGCGTTGCA CAAACAATGA GGAAACTGAC CATTCAGATC TCTTGGGAAC CCTTCATAAT 
CTTTACCAGA TTACTTTTAA TCCTGTGGGA AGAGCAGCTG TTGGTCATGT CTTCAGTCTT 
GAAAAGAATC TCCAGAGTCT TATTACTCTA ATGGAGTACT ATTCCAAAGA GGCTTTAGGG 
GACTCCAAGT CAAAGAAATC GGTAGCTTAT AATTATGCGT GCATACTTGT TTTGTTGGTG 
GTTCAGTCTT CTAGTGATGT CCAGATGCTA GAGCAACATG CAGCGCCTCT CTTGAAGCTT 
TGTAAAGCAG ATGAAAATAA CACGAAATTG CAAGAACTTG GCAAGTGGCT GGAGCCTCTG 
AAAAACCTTA GATTTGAAAT TAATTGTATC CCAAGCCTAA TTGAATATGT TAAACAGAAT 
ATTGATAACT TGATGACCCC AGAAGGAGTT GGTCTACCAA CTGCATTACG TGTCCTCTGT 
AACATAGCGT GCCCACCACC TCCTGTTGAA GGTCAACAGA AAGATCTGAA GTGGAACCTT 
GCAGTTATTC AGCTTTTTTC TGCTGAAGGA ATGGACACCT TTATTCGTGT TCTGCAAAAA 
CTGAACAATA TTCTTACGCA GCCTTGGAGA CTCCATGTCA ACATGGGGAC TACTCTTCAT 
CGAGTTACTA CCATTTCCAT GGCCCGCTGC ACACTCACTC TTCTTAAAAC CATGTTAACA 
GAGCTTCTCA GGGGTGGATC CTTTGAGTTT AAAGACATGC GTGTTCCTTC AGCCCTTGTT 
ACTTTACACA TGCTACTGTG CTCTATCCCT CTCTCAGGTC GTCTGGATAG TGATGAGCAG 
AAGATTCAGA ATGACATCAT TGATATCTTA CTGACTTTTA CGCAGGGAGT TAATGAAAAA 
CTTACAATTT CGGAAGAGAC TTTAGCCAAT AATACTTGGT CTTTAATGTT AAAAGAAGTT 
CTTTCTTCAG TCTTGAGAGT TCCTGAAGGA TTTTTTTCTG GACTCATTCT GCTTTCAGAG 
CTCCTGCCCC TTCCATTGCC CATGCAAACA ACTCAGGTTA TTGAGCCACA TGATATATCA 
GTGGCACTCA ACACTCGCAA ACTGTGGAGT ATGCATCTCC ACGTCCAGGC AAAGCTGCTC 
CAAGAAATAG TTCGTTCTTT CTCTGGCACA ACTTGCCTGC CAATTCAGCA CATGTTGCGG 
CGCATTTGTG TTCAATTATG TGACCTTGCT TCACCAACTG CACTTCTAAT CATGAGAACT 
GTCTTGGATT TGATTGTAGA AGACATGCAA AGCAATTCAG AAGATAAAGA AAAACAGTAT 
ACAAGTCAAA CAACCAGACT TCTGGCTCTT CTTGATGCCC TGGCTTCACA CAAAGCTTGT 
AAATTGGCTA TTTTGCATCT GATAAATGGA ACTATTAAAG GTGATGAACG ATATGCAGAG 
ATTTTCCAGG AATTGTTAGC TCTTCGATCA ACTGGAGAGA GTGTTATTCA TCAACAGTGT 
GTTGAATATG TCATGTCCAT TTTGCAGTCT CTCTGTGATC AGGACATAGC ACTTATTTTG 
CCAAGTTCTT CAGAAGGATC ACTTTCAGAA CTGGAACAGT TGTCCAATTC TTTACCAAAC 
AAGGAATTGA TGGTCTTGAT CTGTGACTGT CTACTGGAAA CAGTAGCCAA TTCTGAGAAT 
AGTTACAACT GCTTACTGAC ATGTGTCAGA ACTATGATGT TTCTTGCAGA ACATGATTAT 
GGATTCTTTC ACTTAAAAAG TTCATTAAGA AAACACAGTA ATGCTTTGCA CCGATTATTG 
AAACGCATTG TTAGTACATT TAGTAAGGAT ACAGGAGAAC TTGCATCTTC TTTTTTGGAA 
TTCATGAGAC AAATACTCAA CTCTGACACA ATGGGATGTT GTGGAGATGA CAGTGGTCTC 
ATGGAAATAG ATGGATCTCA TTCATCAAGA ACATTGAGTC TTAATGCTGC AGAATTAAAA 
CAACTTTTAC AGAGCAAAGA AGAAAGTCCA GAAAGTTTGC TTCTTCAACT GGAGAAGCAT 
ATTTTGGAAC ATTCTAAAGA GGATGACAAC CTGGATTCTT TGCAGGACAA CATAGTTGGT 
CTTAGACAAA TGCTGGAATC TGCAGGTGAT CCTTTACCAC TTAGTGATCA AGATGTGGAA 
CCAGTACTTT CAGCTCCAGA ATCTCTTCAA AATCTCTTTA ATAATAGGAC TGCATATGTG 
CTAGCTGATG TCATGGATGA CCAGCTGAAA TCCATGTGGT TCTCTCCATT CCAGGCTGAA 
GAGATTGAAA CAGATTTAGA TTTGGTAAAA GTTGATCTAA TTGAGCTGTC TGAAAAATGT 
TATAGTGACT TTGATTTGCA AGCCGAATTA GAGCGTTCCT TTTTGTCAGA ACCATCATCT 
CCTGGGAGAA CAAAGACTAC AAAAGGATTT AAACTTGGCA AGCACAAACA TGAGACTTTT 
ATAACATCGA GTGGAAAATC TGAATACATT GAACCTGCCA AGAGAGCACA CATTGTGCCA 
CCTCCCAGAG GAAGAGGCCG GGGAGGATTT GGACAGGGCA TCAGACCCCA TGATATCTTT 
CGTCAGAGGA AACAGAACAC CAGTAGGCCA CCATCTATGC ACGTGGATGA TTTTGTTGCA 
GCTGAAAGCA AAGAAGTGGT CCCACAAGAT GGGTTACCAC CACCAAAAAG GCCACCCAAA 
GTATCACAGA AGATTTCTTC TCGTGGTGGA TTTTCAGGGA ACCGAGGAGG ACGAGGTGCT 
TTTCATAGTC AGAATCGGTT CTTCACTCCA CCTGCTTCAA AAGGAAATTA TAGTCGTCGT 
GAGGGAACAC GAGGTTCTAG CTGGAGTGCG CAGAGTACAC CACGAGGAAC CTATAATGAA 
AGTAGAGGAG GGCAGAGCAA TTTTAGCAGG GGGCCTCTTC CACCCCTTCG GCCACTCAGT 
TCTGCAGGAT ATCGTCCAAG TCCACGCGAC CGTGCTTCCA GAGGTCGGGG TGGACTTGGT 
CCTTCTTGGG CAAGCGCAAA TAGTGGCAGT GGAGGCTCAA GAGGAAAATT TGTTAGTGGA 
GGCAGCGGGA GAGGTCGTCA TGTACGGTCC TTCACACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VIRMA ( XM_023496392.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023352160.1 Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X2, mRNA.

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CloneID	OSa158744
Clone ID Related Accession (Same CDS sequence)	XM_023496392.1 , XM_023496392.2
Accession Version	XM_023496392.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein virilizer homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023496392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGAGTTCCC ATATAGATGT GGTTCGTTTT CCGTGTGTGG TTTATATCAA TGAAGTCCGT 
GTCATTCCAC CAGGAGTAAG AGCTCATAGT AGTCTGCCTG ATAATAGAGC ATATGGGGAA 
ACCTCTCCGC ATACATTTCA GCTAGACTTT TTTTTCAACA ATGTAAGCAA ACCAAGTGCT 
CCCGTTTTTG ATAGGCTGGG AAGTCTGGAC TATGATGAAA ACACCTCTAT CATCTTTAGA 
CCTAATGCAA AGGTAAATAC TGATGGCTTG GTTCTAAGAG GTTGGTACAA CTGTCTGACA 
CTAGCAATAT ATGGATCCGT TGATAGAGTG ATAAGTCATG ATAGAGACTC TCCACCACCA 
CCACCTCCAC CTCCACCTCC TCCACAGCAG CAGCCAAGTG TAAAAAGAAC TCCAAAACAT 
GCAGATGGTG AGAAAGAAGA GCAGTTTAAT GGAAGTCCTC CAAGACCACA GCCCCGAGGA 
CCAAGGACTC CTCCAGGACC CCCTCCACCT GATGATGATG ATGATGATGA ACCTCTGCCA 
GTACCAGTAG TTGGTGAGAA AGAAGATGAT GTTCCTCAAC GAGAAGATTA CTTTGAGCCC 
ATTTCACCTG ATCGGACTTC TGTTCATCAA GAAGGGCAGT ATTCAGATGA AGGTGAAGTA 
GAAGAGGATC ATCATGAGGA AGGTGAAGAT GAAGAAGATG TAGATGTTGA AGAAGAGGAG 
GATGAAGATG AGGAAGATGG TCATACAGTA GAGAGTATCC CAGAAGAAGA AGAAGATGAA 
GAAGAGGAGG AAGAAGGTGA AGAAGAAGAA GGTGAAGAAG AGGAAGATGA AGAAGAAGAA 
GGTGAAGGAG AAGAAGAAGA AGAAGAAGAA GAAGGTGAAG GAGATGATGG CTATGAGCAA 
ATTTCTAGTG ATGAAGATGG AATTGCTGAT TTAGAACGTG AGACTTTTAA ATATCCGAAC 
TTTGATGTTG AATATACTGC TGAGGATTTA GCTTCAGTGC CTCCAATGAC ATATGATCCA 
TATGACAGAG AACTTGTACC ACTTGCATAC TTTAGCTGCC CTTACAAGAC AACTTTTGAA 
ATTGAAGTCA GCAGGATGAA AGACCAAGGT CCTGATAAAG AAAATTCAGG GGCAGTAGAA 
GCATCAGTAA AGTTAGTGGA ACTTTTAGAG TTATACCAGG AAGATAGAGG TCCAAAATGG 
GTCACAGCTT TGGAAGAAGT TCCAGGTTTA ATAGTAAAAG GATTAAGTTA TTTGCAGTTC 
AAAAATGCAC AACAAGACTA CATTGGCCAG CTGGTAGATT GGACCATGCA AGCCTTGAAT 
TTACAAGTAG CTCTCCGTCA GCCCATTGCC TTAAATGTCC GGCAGCTCAA AGCTGGGACT 
AAATTAGTAT CCTCATTAGC AGAATGTGGG CCTCAAGGAG TGATGGGACT TTTACAAGCA 
GGAGTTATCA GTGGATTATT TGAACTGCTA TTTGCTGACC ATGTATCATC TTCTCTTAAG 
CTAAATGCTT TTAAAGCTTT GGACAGTATT ATTAGTATGA CTGAAGGCAT GGAGGATTTT 
TTAAGAGGCA GACAGGACTT ACATGAAAAA AGTGGTTATC AGAAACTTCT GGAGCTCATT 
CTCTTGGATC AGACCGTGAG AGTAGTTACT GCTGGTTCTG CAATTCTCCA GAAATGTCAC 
TTCTATGAGA TTCTGTCTGA AATTAAAAGA CTTGGTGATC AGTTGGCCGA GAGGTCTTCT 
ATTCCTAACC ACAGCGAATC TGAGCAGGAC GCAGACCCTG GACTTGAGAG AACAAATCCT 
GAATATGAAA ATGAAGTGGA AGCTTCTTTG GATATAGTTC ATCTTTTGGA ATCCTCTCTT 
ATAACTGAAG GGGAAATAGA AAAGCTTATT AATCTCTTGG ATGAAATCTT CCACTTAATG 
GAGACTGCAC CTCACACGAT GGTTCAGCCT CCTGTGAAGT CATTCCCGAC AATAGCAAGG 
ATTACTGGAC CTCCAGAGAG AGATGATCCT TATCCTGTCT TATTCAGATA CCTCCACAGC 
CACCACTTCC TAGAATTGGT CACCTTACTG CTGTCAATTC CAGCAATAAG CGCTCACCCT 
GCTGTGCTGC AGGCTATGAG AGATGTTTTG AAGTTTCTTA CACAGTCGCA AAAAGGTCTT 
CTTTTTTTCC TCTCGGAACA TGAGGCGACT AACTTGCTGA TTAGAGCTCT TTGTCACCTT 
CTTGATCAAG AAGAAGAAGA AGGTCTCCAG GCTGATGGTG TTATTGATGA TACATTTGCT 
TTGTGGCTAC AGTATTCAAC TCAGACTTTG CAGTTTATTA CAGAACTATT TTGTCATTTT 
CAGCGTTGCA CAAACAATGA GGAAACTGAC CATTCAGATC TCTTGGGAAC CCTTCATAAT 
CTTTACCAGA TTACTTTTAA TCCTGTGGGA AGAGCAGCTG TTGGTCATGT CTTCAGTCTT 
GAAAAGAATC TCCAGAGTCT TATTACTCTA ATGGAGTACT ATTCCAAAGA GGCTTTAGGG 
GACTCCAAGT CAAAGAAATC GGTAGCTTAT AATTATGCGT GCATACTTGT TTTGTTGGTG 
GTTCAGTCTT CTAGTGATGT CCAGATGCTA GAGCAACATG CAGCGCCTCT CTTGAAGCTT 
TGTAAAGCAG ATGAAAATAA CACGAAATTG CAAGAACTTG GCAAGTGGCT GGAGCCTCTG 
AAAAACCTTA GATTTGAAAT TAATTGTATC CCAAGCCTAA TTGAATATGT TAAACAGAAT 
ATTGATAACT TGATGACCCC AGAAGGAGTT GGTCTACCAA CTGCATTACG TGTCCTCTGT 
AACATAGCGT GCCCACCACC TCCTGTTGAA GGTCAACAGA AAGATCTGAA GTGGAACCTT 
GCAGTTATTC AGCTTTTTTC TGCTGAAGGA ATGGACACCT TTATTCGTGT TCTGCAAAAA 
CTGAACAATA TTCTTACGCA GCCTTGGAGA CTCCATGTCA ACATGGGGAC TACTCTTCAT 
CGAGTTACTA CCATTTCCAT GGCCCGCTGC ACACTCACTC TTCTTAAAAC CATGTTAACA 
GAGCTTCTCA GGGGTGGATC CTTTGAGTTT AAAGACATGC GTGTTCCTTC AGCCCTTGTT 
ACTTTACACA TGCTACTGTG CTCTATCCCT CTCTCAGGTC GTCTGGATAG TGATGAGCAG 
AAGATTCAGA ATGACATCAT TGATATCTTA CTGACTTTTA CGCAGGGAGT TAATGAAAAA 
CTTACAATTT CGGAAGAGAC TTTAGCCAAT AATACTTGGT CTTTAATGTT AAAAGAAGTT 
CTTTCTTCAG TCTTGAGAGT TCCTGAAGGA TTTTTTTCTG GACTCATTCT GCTTTCAGAG 
CTCCTGCCCC TTCCATTGCC CATGCAAACA ACTCAGGTTA TTGAGCCACA TGATATATCA 
GTGGCACTCA ACACTCGCAA ACTGTGGAGT ATGCATCTCC ACGTCCAGGC AAAGCTGCTC 
CAAGAAATAG TTCGTTCTTT CTCTGGCACA ACTTGCCTGC CAATTCAGCA CATGTTGCGG 
CGCATTTGTG TTCAATTATG TGACCTTGCT TCACCAACTG CACTTCTAAT CATGAGAACT 
GTCTTGGATT TGATTGTAGA AGACATGCAA AGCAATTCAG AAGATAAAGA AAAACAGTAT 
ACAAGTCAAA CAACCAGACT TCTGGCTCTT CTTGATGCCC TGGCTTCACA CAAAGCTTGT 
AAATTGGCTA TTTTGCATCT GATAAATGGA ACTATTAAAG GTGATGAACG ATATGCAGAG 
ATTTTCCAGG AATTGTTAGC TCTTCGATCA ACTGGAGAGA GTGTTATTCA TCAACAGTGT 
GTTGAATATG TCATGTCCAT TTTGCAGTCT CTCTGTGATC AGGACATAGC ACTTATTTTG 
CCAAGTTCTT CAGAAGGATC ACTTTCAGAA CTGGAACAGT TGTCCAATTC TTTACCAAAC 
AAGGAATTGA TGGTCTTGAT CTGTGACTGT CTACTGGAAA CAGTAGCCAA TTCTGAGAAT 
AGTTACAACT GCTTACTGAC ATGTGTCAGA ACTATGATGT TTCTTGCAGA ACATGATTAT 
GGATTCTTTC ACTTAAAAAG TTCATTAAGA AAACACAGTA ATGCTTTGCA CCGATTATTG 
AAACGCATTG TTAGTACATT TAGTAAGGAT ACAGGAGAAC TTGCATCTTC TTTTTTGGAA 
TTCATGAGAC AAATACTCAA CTCTGACACA ATGGGATGTT GTGGAGATGA CAGTGGTCTC 
ATGGAAATAG ATGGATCTCA TTCATCAAGA ACATTGAGTC TTAATGCTGC AGAATTAAAA 
CAACTTTTAC AGAGCAAAGA AGAAAGTCCA GAAAGTTTGC TTCTTCAACT GGAGAAGCAT 
ATTTTGGAAC ATTCTAAAGA GGATGACAAC CTGGATTCTT TGCAGGACAA CATAGTTGGT 
CTTAGACAAA TGCTGGAATC TGCAGGTGAT CCTTTACCAC TTAGTGATCA AGATGTGGAA 
CCAGTACTTT CAGCTCCAGA ATCTCTTCAA AATCTCTTTA ATAATAGGAC TGCATATGTG 
CTAGCTGATG TCATGGATGA CCAGCTGAAA TCCATGTGGT TCTCTCCATT CCAGGCTGAA 
GAGATTGAAA CAGATTTAGA TTTGGTAAAA GTTGATCTAA TTGAGCTGTC TGAAAAATGT 
TATAGTGACT TTGATTTGCA AGCCGAATTA GAGCGTTCCT TTTTGTCAGA ACCATCATCT 
CCTGGGAGAA CAAAGACTAC AAAAGGATTT AAACTTGGCA AGCACAAACA TGAGACTTTT 
ATAACATCGA GTGGAAAATC TGAATACATT GAACCTGCCA AGAGAGCACA CATTGTGCCA 
CCTCCCAGAG GAAGAGGCCG GGGAGGATTT GGACAGGGCA TCAGACCCCA TGATATCTTT 
CGTCAGAGGA AACAGAACAC CAGTAGGCCA CCATCTATGC ACGTGGATGA TTTTGTTGCA 
GCTGAAAGCA AAGAAGTGGT CCCACAAGAT GGGTTACCAC CACCAAAAAG GCCACCCAAA 
GTATCACAGA AGATTTCTTC TCGTGGTGGA TTTTCAGGGA ACCGAGGAGG ACGAGGTGCT 
TTTCATAGTC AGAATCGGTT CTTCACTCCA CCTGCTTCAA AAGGAAATTA TAGTCGTCGT 
GAGGGAACAC GAGGTTCTAG CTGGAGTGCG CAGAGTACAC CACGAGGAAC CTATAATGAA 
AGTAGAGGAG GGCAGAGCAA TTTTAGCAGG GGGCCTCTTC CACCCCTTCG GCCACTCAGT 
TCTGCAGGAT ATCGTCCAAG TCCACGCGAC CGTGCTTCCA GAGGTCGGGG TGGACTTGGT 
CCTTCTTGGG CAAGCGCAAA TAGTGGCAGT GGAGGCTCAA GAGGAAAATT TGTTAGTGGA 
GGCAGCGGGA GAGGTCGTCA TGTACGGTCC TTCACACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023352160.1
CDS	41..5542
Translation	MAVDSAMELLFLDTFKHPSAESSHIDVVRFPCVVYINEVRVIPPGVRAHSSLPDNRAYGETSPHTFQLDFFFNNVSKPSAPVFDRLGSLDYDENTSIIFRPNAKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQQQPSVKRTPKHADGEKEEQFNGSPPRPQPRGPRTPPGPPPPDDDDDDEPLPVPVVGEKEDDVPQREDYFEPISPDRTSVHQEGQYSDEGEVEEDHHEEGEDEEDVDVEEEEDEDEEDGHTVESIPEEEEDEEEEEEGEEEEGEEEEDEEEEGEGEEEEEEEEGEGDDGYEQISSDEDGIADLERETFKYPNFDVEYTAEDLASVPPMTYDPYDRELVPLAYFSCPYKTTFEIEVSRMKDQGPDKENSGAVEASVKLVELLELYQEDRGPKWVTALEEVPGLIVKGLSYLQFKNAQQDYIGQLVDWTMQALNLQVALRQPIALNVRQLKAGTKLVSSLAECGPQGVMGLLQAGVISGLFELLFADHVSSSLKLNAFKALDSIISMTEGMEDFLRGRQDLHEKSGYQKLLELILLDQTVRVVTAGSAILQKCHFYEILSEIKRLGDQLAERSSIPNHSESEQDADPGLERTNPEYENEVEASLDIVHLLESSLITEGEIEKLINLLDEIFHLMETAPHTMVQPPVKSFPTIARITGPPERDDPYPVLFRYLHSHHFLELVTLLLSIPAISAHPAVLQAMRDVLKFLTQSQKGLLFFLSEHEATNLLIRALCHLLDQEEEEGLQADGVIDDTFALWLQYSTQTLQFITELFCHFQRCTNNEETDHSDLLGTLHNLYQITFNPVGRAAVGHVFSLEKNLQSLITLMEYYSKEALGDSKSKKSVAYNYACILVLLVVQSSSDVQMLEQHAAPLLKLCKADENNTKLQELGKWLEPLKNLRFEINCIPSLIEYVKQNIDNLMTPEGVGLPTALRVLCNIACPPPPVEGQQKDLKWNLAVIQLFSAEGMDTFIRVLQKLNNILTQPWRLHVNMGTTLHRVTTISMARCTLTLLKTMLTELLRGGSFEFKDMRVPSALVTLHMLLCSIPLSGRLDSDEQKIQNDIIDILLTFTQGVNEKLTISEETLANNTWSLMLKEVLSSVLRVPEGFFSGLILLSELLPLPLPMQTTQVIEPHDISVALNTRKLWSMHLHVQAKLLQEIVRSFSGTTCLPIQHMLRRICVQLCDLASPTALLIMRTVLDLIVEDMQSNSEDKEKQYTSQTTRLLALLDALASHKACKLAILHLINGTIKGDERYAEIFQELLALRSTGESVIHQQCVEYVMSILQSLCDQDIALILPSSSEGSLSELEQLSNSLPNKELMVLICDCLLETVANSENSYNCLLTCVRTMMFLAEHDYGFFHLKSSLRKHSNALHRLLKRIVSTFSKDTGELASSFLEFMRQILNSDTMGCCGDDSGLMEIDGSHSSRTLSLNAAELKQLLQSKEESPESLLLQLEKHILEHSKEDDNLDSLQDNIVGLRQMLESAGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFSPFQAEEIETDLDLVKVDLIELSEKCYSDFDLQAELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHIVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGLPPPKRPPKVSQKISSRGGFSGNRGGRGAFHSQNRFFTPPASKGNYSRREGTRGSSWSAQSTPRGTYNESRGGQSNFSRGPLPPLRPLSSAGYRPSPRDRASRGRGGLGPSWASANSGSGGSRGKFVSGGSGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_023496392.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023496392.2

ORF Insert Sequence:

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGAGTTCCC ATATAGATGT GGTTCGTTTT CCGTGTGTGG TTTATATCAA TGAAGTCCGT 
GTCATTCCAC CAGGAGTAAG AGCTCATAGT AGTCTGCCTG ATAATAGAGC ATATGGGGAA 
ACCTCTCCGC ATACATTTCA GCTAGACTTT TTTTTCAACA ATGTAAGCAA ACCAAGTGCT 
CCCGTTTTTG ATAGGCTGGG AAGTCTGGAC TATGATGAAA ACACCTCTAT CATCTTTAGA 
CCTAATGCAA AGGTAAATAC TGATGGCTTG GTTCTAAGAG GTTGGTACAA CTGTCTGACA 
CTAGCAATAT ATGGATCCGT TGATAGAGTG ATAAGTCATG ATAGAGACTC TCCACCACCA 
CCACCTCCAC CTCCACCTCC TCCACAGCAG CAGCCAAGTG TAAAAAGAAC TCCAAAACAT 
GCAGATGGTG AGAAAGAAGA GCAGTTTAAT GGAAGTCCTC CAAGACCACA GCCCCGAGGA 
CCAAGGACTC CTCCAGGACC CCCTCCACCT GATGATGATG ATGATGATGA ACCTCTGCCA 
GTACCAGTAG TTGGTGAGAA AGAAGATGAT GTTCCTCAAC GAGAAGATTA CTTTGAGCCC 
ATTTCACCTG ATCGGACTTC TGTTCATCAA GAAGGGCAGT ATTCAGATGA AGGTGAAGTA 
GAAGAGGATC ATCATGAGGA AGGTGAAGAT GAAGAAGATG TAGATGTTGA AGAAGAGGAG 
GATGAAGATG AGGAAGATGG TCATACAGTA GAGAGTATCC CAGAAGAAGA AGAAGATGAA 
GAAGAGGAGG AAGAAGGTGA AGAAGAAGAA GGTGAAGAAG AGGAAGATGA AGAAGAAGAA 
GGTGAAGGAG AAGAAGAAGA AGAAGAAGAA GAAGGTGAAG GAGATGATGG CTATGAGCAA 
ATTTCTAGTG ATGAAGATGG AATTGCTGAT TTAGAACGTG AGACTTTTAA ATATCCGAAC 
TTTGATGTTG AATATACTGC TGAGGATTTA GCTTCAGTGC CTCCAATGAC ATATGATCCA 
TATGACAGAG AACTTGTACC ACTTGCATAC TTTAGCTGCC CTTACAAGAC AACTTTTGAA 
ATTGAAGTCA GCAGGATGAA AGACCAAGGT CCTGATAAAG AAAATTCAGG GGCAGTAGAA 
GCATCAGTAA AGTTAGTGGA ACTTTTAGAG TTATACCAGG AAGATAGAGG TCCAAAATGG 
GTCACAGCTT TGGAAGAAGT TCCAGGTTTA ATAGTAAAAG GATTAAGTTA TTTGCAGTTC 
AAAAATGCAC AACAAGACTA CATTGGCCAG CTGGTAGATT GGACCATGCA AGCCTTGAAT 
TTACAAGTAG CTCTCCGTCA GCCCATTGCC TTAAATGTCC GGCAGCTCAA AGCTGGGACT 
AAATTAGTAT CCTCATTAGC AGAATGTGGG CCTCAAGGAG TGATGGGACT TTTACAAGCA 
GGAGTTATCA GTGGATTATT TGAACTGCTA TTTGCTGACC ATGTATCATC TTCTCTTAAG 
CTAAATGCTT TTAAAGCTTT GGACAGTATT ATTAGTATGA CTGAAGGCAT GGAGGATTTT 
TTAAGAGGCA GACAGGACTT ACATGAAAAA AGTGGTTATC AGAAACTTCT GGAGCTCATT 
CTCTTGGATC AGACCGTGAG AGTAGTTACT GCTGGTTCTG CAATTCTCCA GAAATGTCAC 
TTCTATGAGA TTCTGTCTGA AATTAAAAGA CTTGGTGATC AGTTGGCCGA GAGGTCTTCT 
ATTCCTAACC ACAGCGAATC TGAGCAGGAC GCAGACCCTG GACTTGAGAG AACAAATCCT 
GAATATGAAA ATGAAGTGGA AGCTTCTTTG GATATAGTTC ATCTTTTGGA ATCCTCTCTT 
ATAACTGAAG GGGAAATAGA AAAGCTTATT AATCTCTTGG ATGAAATCTT CCACTTAATG 
GAGACTGCAC CTCACACGAT GGTTCAGCCT CCTGTGAAGT CATTCCCGAC AATAGCAAGG 
ATTACTGGAC CTCCAGAGAG AGATGATCCT TATCCTGTCT TATTCAGATA CCTCCACAGC 
CACCACTTCC TAGAATTGGT CACCTTACTG CTGTCAATTC CAGCAATAAG CGCTCACCCT 
GCTGTGCTGC AGGCTATGAG AGATGTTTTG AAGTTTCTTA CACAGTCGCA AAAAGGTCTT 
CTTTTTTTCC TCTCGGAACA TGAGGCGACT AACTTGCTGA TTAGAGCTCT TTGTCACCTT 
CTTGATCAAG AAGAAGAAGA AGGTCTCCAG GCTGATGGTG TTATTGATGA TACATTTGCT 
TTGTGGCTAC AGTATTCAAC TCAGACTTTG CAGTTTATTA CAGAACTATT TTGTCATTTT 
CAGCGTTGCA CAAACAATGA GGAAACTGAC CATTCAGATC TCTTGGGAAC CCTTCATAAT 
CTTTACCAGA TTACTTTTAA TCCTGTGGGA AGAGCAGCTG TTGGTCATGT CTTCAGTCTT 
GAAAAGAATC TCCAGAGTCT TATTACTCTA ATGGAGTACT ATTCCAAAGA GGCTTTAGGG 
GACTCCAAGT CAAAGAAATC GGTAGCTTAT AATTATGCGT GCATACTTGT TTTGTTGGTG 
GTTCAGTCTT CTAGTGATGT CCAGATGCTA GAGCAACATG CAGCGCCTCT CTTGAAGCTT 
TGTAAAGCAG ATGAAAATAA CACGAAATTG CAAGAACTTG GCAAGTGGCT GGAGCCTCTG 
AAAAACCTTA GATTTGAAAT TAATTGTATC CCAAGCCTAA TTGAATATGT TAAACAGAAT 
ATTGATAACT TGATGACCCC AGAAGGAGTT GGTCTACCAA CTGCATTACG TGTCCTCTGT 
AACATAGCGT GCCCACCACC TCCTGTTGAA GGTCAACAGA AAGATCTGAA GTGGAACCTT 
GCAGTTATTC AGCTTTTTTC TGCTGAAGGA ATGGACACCT TTATTCGTGT TCTGCAAAAA 
CTGAACAATA TTCTTACGCA GCCTTGGAGA CTCCATGTCA ACATGGGGAC TACTCTTCAT 
CGAGTTACTA CCATTTCCAT GGCCCGCTGC ACACTCACTC TTCTTAAAAC CATGTTAACA 
GAGCTTCTCA GGGGTGGATC CTTTGAGTTT AAAGACATGC GTGTTCCTTC AGCCCTTGTT 
ACTTTACACA TGCTACTGTG CTCTATCCCT CTCTCAGGTC GTCTGGATAG TGATGAGCAG 
AAGATTCAGA ATGACATCAT TGATATCTTA CTGACTTTTA CGCAGGGAGT TAATGAAAAA 
CTTACAATTT CGGAAGAGAC TTTAGCCAAT AATACTTGGT CTTTAATGTT AAAAGAAGTT 
CTTTCTTCAG TCTTGAGAGT TCCTGAAGGA TTTTTTTCTG GACTCATTCT GCTTTCAGAG 
CTCCTGCCCC TTCCATTGCC CATGCAAACA ACTCAGGTTA TTGAGCCACA TGATATATCA 
GTGGCACTCA ACACTCGCAA ACTGTGGAGT ATGCATCTCC ACGTCCAGGC AAAGCTGCTC 
CAAGAAATAG TTCGTTCTTT CTCTGGCACA ACTTGCCTGC CAATTCAGCA CATGTTGCGG 
CGCATTTGTG TTCAATTATG TGACCTTGCT TCACCAACTG CACTTCTAAT CATGAGAACT 
GTCTTGGATT TGATTGTAGA AGACATGCAA AGCAATTCAG AAGATAAAGA AAAACAGTAT 
ACAAGTCAAA CAACCAGACT TCTGGCTCTT CTTGATGCCC TGGCTTCACA CAAAGCTTGT 
AAATTGGCTA TTTTGCATCT GATAAATGGA ACTATTAAAG GTGATGAACG ATATGCAGAG 
ATTTTCCAGG AATTGTTAGC TCTTCGATCA ACTGGAGAGA GTGTTATTCA TCAACAGTGT 
GTTGAATATG TCATGTCCAT TTTGCAGTCT CTCTGTGATC AGGACATAGC ACTTATTTTG 
CCAAGTTCTT CAGAAGGATC ACTTTCAGAA CTGGAACAGT TGTCCAATTC TTTACCAAAC 
AAGGAATTGA TGGTCTTGAT CTGTGACTGT CTACTGGAAA CAGTAGCCAA TTCTGAGAAT 
AGTTACAACT GCTTACTGAC ATGTGTCAGA ACTATGATGT TTCTTGCAGA ACATGATTAT 
GGATTCTTTC ACTTAAAAAG TTCATTAAGA AAACACAGTA ATGCTTTGCA CCGATTATTG 
AAACGCATTG TTAGTACATT TAGTAAGGAT ACAGGAGAAC TTGCATCTTC TTTTTTGGAA 
TTCATGAGAC AAATACTCAA CTCTGACACA ATGGGATGTT GTGGAGATGA CAGTGGTCTC 
ATGGAAATAG ATGGATCTCA TTCATCAAGA ACATTGAGTC TTAATGCTGC AGAATTAAAA 
CAACTTTTAC AGAGCAAAGA AGAAAGTCCA GAAAGTTTGC TTCTTCAACT GGAGAAGCAT 
ATTTTGGAAC ATTCTAAAGA GGATGACAAC CTGGATTCTT TGCAGGACAA CATAGTTGGT 
CTTAGACAAA TGCTGGAATC TGCAGGTGAT CCTTTACCAC TTAGTGATCA AGATGTGGAA 
CCAGTACTTT CAGCTCCAGA ATCTCTTCAA AATCTCTTTA ATAATAGGAC TGCATATGTG 
CTAGCTGATG TCATGGATGA CCAGCTGAAA TCCATGTGGT TCTCTCCATT CCAGGCTGAA 
GAGATTGAAA CAGATTTAGA TTTGGTAAAA GTTGATCTAA TTGAGCTGTC TGAAAAATGT 
TATAGTGACT TTGATTTGCA AGCCGAATTA GAGCGTTCCT TTTTGTCAGA ACCATCATCT 
CCTGGGAGAA CAAAGACTAC AAAAGGATTT AAACTTGGCA AGCACAAACA TGAGACTTTT 
ATAACATCGA GTGGAAAATC TGAATACATT GAACCTGCCA AGAGAGCACA CATTGTGCCA 
CCTCCCAGAG GAAGAGGCCG GGGAGGATTT GGACAGGGCA TCAGACCCCA TGATATCTTT 
CGTCAGAGGA AACAGAACAC CAGTAGGCCA CCATCTATGC ACGTGGATGA TTTTGTTGCA 
GCTGAAAGCA AAGAAGTGGT CCCACAAGAT GGGTTACCAC CACCAAAAAG GCCACCCAAA 
GTATCACAGA AGATTTCTTC TCGTGGTGGA TTTTCAGGGA ACCGAGGAGG ACGAGGTGCT 
TTTCATAGTC AGAATCGGTT CTTCACTCCA CCTGCTTCAA AAGGAAATTA TAGTCGTCGT 
GAGGGAACAC GAGGTTCTAG CTGGAGTGCG CAGAGTACAC CACGAGGAAC CTATAATGAA 
AGTAGAGGAG GGCAGAGCAA TTTTAGCAGG GGGCCTCTTC CACCCCTTCG GCCACTCAGT 
TCTGCAGGAT ATCGTCCAAG TCCACGCGAC CGTGCTTCCA GAGGTCGGGG TGGACTTGGT 
CCTTCTTGGG CAAGCGCAAA TAGTGGCAGT GGAGGCTCAA GAGGAAAATT TGTTAGTGGA 
GGCAGCGGGA GAGGTCGTCA TGTACGGTCC TTCACACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VIRMA ( XM_023496393.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023352161.1 Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X4, mRNA.

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CloneID	OSa158745
Clone ID Related Accession (Same CDS sequence)	XM_023496393.1
Accession Version	XM_023496393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-10
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein virilizer homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823376.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGATGGTG AGAAAGAAGA GCAGTTTAAT GGAAGTCCTC CAAGACCACA GCCCCGAGGA 
CCAAGGACTC CTCCAGGACC CCCTCCACCT GATGATGATG ATGATGATGA ACCTCTGCCA 
GTACCAGTAG TTGGTGAGAA AGAAGATGAT GTTCCTCAAC GAGAAGATTA CTTTGAGCCC 
ATTTCACCTG ATCGGACTTC TGTTCATCAA GAAGGGCAGT ATTCAGATGA AGGTGAAGTA 
GAAGAGGATC ATCATGAGGA AGGTGAAGAT GAAGAAGATG TAGATGTTGA AGAAGAGGAG 
GATGAAGATG AGGAAGATGG TCATACAGTA GAGAGTATCC CAGAAGAAGA AGAAGATGAA 
GAAGAGGAGG AAGAAGGTGA AGAAGAAGAA GGTGAAGAAG AGGAAGATGA AGAAGAAGAA 
GGTGAAGGAG AAGAAGAAGA AGAAGAAGAA GAAGGTGAAG GAGATGATGG CTATGAGCAA 
ATTTCTAGTG ATGAAGATGG AATTGCTGAT TTAGAACGTG AGACTTTTAA ATATCCGAAC 
TTTGATGTTG AATATACTGC TGAGGATTTA GCTTCAGTGC CTCCAATGAC ATATGATCCA 
TATGACAGAG AACTTGTACC ACTTGCATAC TTTAGCTGCC CTTACAAGAC AACTTTTGAA 
ATTGAAGTCA GCAGGATGAA AGACCAAGGT CCTGATAAAG AAAATTCAGG GGCAGTAGAA 
GCATCAGTAA AGTTAGTGGA ACTTTTAGAG TTATACCAGG AAGATAGAGG TCCAAAATGG 
GTCACAGCTT TGGAAGAAGT TCCAGGTTTA ATAGTAAAAG GATTAAGTTA TTTGCAGTTC 
AAAAATGCAC AACAAGACTA CATTGGCCAG CTGGTAGATT GGACCATGCA AGCCTTGAAT 
TTACAAGTAG CTCTCCGTCA GCCCATTGCC TTAAATGTCC GGCAGCTCAA AGCTGGGACT 
AAATTAGTAT CCTCATTAGC AGAATGTGGG CCTCAAGGAG TGATGGGACT TTTACAAGCA 
GGAGTTATCA GTGGATTATT TGAACTGCTA TTTGCTGACC ATGTATCATC TTCTCTTAAG 
CTAAATGCTT TTAAAGCTTT GGACAGTATT ATTAGTATGA CTGAAGGCAT GGAGGATTTT 
TTAAGAGGCA GACAGGACTT ACATGAAAAA AGTGGTTATC AGAAACTTCT GGAGCTCATT 
CTCTTGGATC AGACCGTGAG AGTAGTTACT GCTGGTTCTG CAATTCTCCA GAAATGTCAC 
TTCTATGAGA TTCTGTCTGA AATTAAAAGA CTTGGTGATC AGTTGGCCGA GAGGTCTTCT 
ATTCCTAACC ACAGCGAATC TGAGCAGGAC GCAGACCCTG GACTTGAGAG AACAAATCCT 
GAATATGAAA ATGAAGTGGA AGCTTCTTTG GATATAGTTC ATCTTTTGGA ATCCTCTCTT 
ATAACTGAAG GGGAAATAGA AAAGCTTATT AATCTCTTGG ATGAAATCTT CCACTTAATG 
GAGACTGCAC CTCACACGAT GGTTCAGCCT CCTGTGAAGT CATTCCCGAC AATAGCAAGG 
ATTACTGGAC CTCCAGAGAG AGATGATCCT TATCCTGTCT TATTCAGATA CCTCCACAGC 
CACCACTTCC TAGAATTGGT CACCTTACTG CTGTCAATTC CAGCAATAAG CGCTCACCCT 
GCTGTGCTGC AGGCTATGAG AGATGTTTTG AAGTTTCTTA CACAGTCGCA AAAAGGTCTT 
CTTTTTTTCC TCTCGGAACA TGAGGCGACT AACTTGCTGA TTAGAGCTCT TTGTCACCTT 
CTTGATCAAG AAGAAGAAGA AGGTCTCCAG GCTGATGGTG TTATTGATGA TACATTTGCT 
TTGTGGCTAC AGTATTCAAC TCAGACTTTG CAGTTTATTA CAGAACTATT TTGTCATTTT 
CAGCGTTGCA CAAACAATGA GGAAACTGAC CATTCAGATC TCTTGGGAAC CCTTCATAAT 
CTTTACCAGA TTACTTTTAA TCCTGTGGGA AGAGCAGCTG TTGGTCATGT CTTCAGTCTT 
GAAAAGAATC TCCAGAGTCT TATTACTCTA ATGGAGTACT ATTCCAAAGA GGCTTTAGGG 
GACTCCAAGT CAAAGAAATC GGTAGCTTAT AATTATGCGT GCATACTTGT TTTGTTGGTG 
GTTCAGTCTT CTAGTGATGT CCAGATGCTA GAGCAACATG CAGCGCCTCT CTTGAAGCTT 
TGTAAAGCAG ATGAAAATAA CACGAAATTG CAAGAACTTG GCAAGTGGCT GGAGCCTCTG 
AAAAACCTTA GATTTGAAAT TAATTGTATC CCAAGCCTAA TTGAATATGT TAAACAGAAT 
ATTGATAACT TGATGACCCC AGAAGGAGTT GGTCTACCAA CTGCATTACG TGTCCTCTGT 
AACATAGCGT GCCCACCACC TCCTGTTGAA GGTCAACAGA AAGATCTGAA GTGGAACCTT 
GCAGTTATTC AGCTTTTTTC TGCTGAAGGA ATGGACACCT TTATTCGTGT TCTGCAAAAA 
CTGAACAATA TTCTTACGCA GCCTTGGAGA CTCCATGTCA ACATGGGGAC TACTCTTCAT 
CGAGTTACTA CCATTTCCAT GGCCCGCTGC ACACTCACTC TTCTTAAAAC CATGTTAACA 
GAGCTTCTCA GGGGTGGATC CTTTGAGTTT AAAGACATGC GTGTTCCTTC AGCCCTTGTT 
ACTTTACACA TGCTACTGTG CTCTATCCCT CTCTCAGGTC GTCTGGATAG TGATGAGCAG 
AAGATTCAGA ATGACATCAT TGATATCTTA CTGACTTTTA CGCAGGGAGT TAATGAAAAA 
CTTACAATTT CGGAAGAGAC TTTAGCCAAT AATACTTGGT CTTTAATGTT AAAAGAAGTT 
CTTTCTTCAG TCTTGAGAGT TCCTGAAGGA TTTTTTTCTG GACTCATTCT GCTTTCAGAG 
CTCCTGCCCC TTCCATTGCC CATGCAAACA ACTCAGGTTA TTGAGCCACA TGATATATCA 
GTGGCACTCA ACACTCGCAA ACTGTGGAGT ATGCATCTCC ACGTCCAGGC AAAGCTGCTC 
CAAGAAATAG TTCGTTCTTT CTCTGGCACA ACTTGCCTGC CAATTCAGCA CATGTTGCGG 
CGCATTTGTG TTCAATTATG TGACCTTGCT TCACCAACTG CACTTCTAAT CATGAGAACT 
GTCTTGGATT TGATTGTAGA AGACATGCAA AGCAATTCAG AAGATAAAGA AAAACAGTAT 
ACAAGTCAAA CAACCAGACT TCTGGCTCTT CTTGATGCCC TGGCTTCACA CAAAGCTTGT 
AAATTGGCTA TTTTGCATCT GATAAATGGA ACTATTAAAG GTGATGAACG ATATGCAGAG 
ATTTTCCAGG AATTGTTAGC TCTTCGATCA ACTGGAGAGA GTGTTATTCA TCAACAGTGT 
GTTGAATATG TCATGTCCAT TTTGCAGTCT CTCTGTGATC AGGACATAGC ACTTATTTTG 
CCAAGTTCTT CAGAAGGATC ACTTTCAGAA CTGGAACAGT TGTCCAATTC TTTACCAAAC 
AAGGAATTGA TGGTCTTGAT CTGTGACTGT CTACTGGAAA CAGTAGCCAA TTCTGAGAAT 
AGTTACAACT GCTTACTGAC ATGTGTCAGA ACTATGATGT TTCTTGCAGA ACATGATTAT 
GGATTCTTTC ACTTAAAAAG TTCATTAAGA AAACACAGTA ATGCTTTGCA CCGATTATTG 
AAACGCATTG TTAGTACATT TAGTAAGGAT ACAGGAGAAC TTGCATCTTC TTTTTTGGAA 
TTCATGAGAC AAATACTCAA CTCTGACACA ATGGGATGTT GTGGAGATGA CAGTGGTCTC 
ATGGAAATAG ATGGATCTCA TTCATCAAGA ACATTGAGTC TTAATGCTGC AGAATTAAAA 
CAACTTTTAC AGAGCAAAGA AGAAAGTCCA GAAAGTTTGC TTCTTCAACT GGAGAAGCAT 
ATTTTGGAAC ATTCTAAAGA GGATGACAAC CTGGATTCTT TGCAGGACAA CATAGTTGGT 
CTTAGACAAA TGCTGGAATC TGCAGGTGAT CCTTTACCAC TTAGTGATCA AGATGTGGAA 
CCAGTACTTT CAGCTCCAGA ATCTCTTCAA AATCTCTTTA ATAATAGGAC TGCATATGTG 
CTAGCTGATG TCATGGATGA CCAGCTGAAA TCCATGTGGT TCTCTCCATT CCAGGCTGAA 
GAGATTGAAA CAGATTTAGA TTTGGTAAAA GTTGATCTAA TTGAGCTGTC TGAAAAATGT 
TATAGTGACT TTGATTTGCA AGCCGAATTA GAGCGTTCCT TTTTGTCAGA ACCATCATCT 
CCTGGGAGAA CAAAGACTAC AAAAGGATTT AAACTTGGCA AGCACAAACA TGAGACTTTT 
ATAACATCGA GTGGAAAATC TGAATACATT GAACCTGCCA AGAGAGCACA CATTGTGCCA 
CCTCCCAGAG GAAGAGGCCG GGGAGGATTT GGACAGGGCA TCAGACCCCA TGATATCTTT 
CGTCAGAGGA AACAGAACAC CAGTAGGCCA CCATCTATGC ACGTGGATGA TTTTGTTGCA 
GCTGAAAGCA AAGAAGTGGT CCCACAAGAT GGGTTACCAC CACCAAAAAG GCCACCCAAA 
GTATCACAGA AGATTTCTTC TCGTGGTGGA TTTTCAGGGA ACCGAGGAGG ACGAGGTGCT 
TTTCATAGTC AGAATCGGTT CTTCACTCCA CCTGCTTCAA AAGGAAATTA TAGTCGTCGT 
GAGGGAACAC GAGGTTCTAG CTGGAGTGCG CAGAGTACAC CACGAGGAAC CTATAATGAA 
AGTAGAGGAG GGCAGAGCAA TTTTAGCAGG GGGCCTCTTC CACCCCTTCG GCCACTCAGT 
TCTGCAGGAT ATCGTCCAAG TCCACGCGAC CGTGCTTCCA GAGGTCGGGG TGGACTTGGT 
CCTTCTTGGG CAAGCGCAAA TAGTGGCAGT GGAGGCTCAA GAGGAAAATT TGTTAGTGGA 
GGCAGCGGGA GAGGTCGTCA TGTACGGTCC TTCACACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023352161.1
CDS	12..5513
Translation	MAVDSAMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSSLPDNRAYGETSPHTFQLDFFFNNVSKPSAPVFDRLGSLDYDENTSIIFRPNAKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQQQPSVKRTPKHDGEKEEQFNGSPPRPQPRGPRTPPGPPPPDDDDDDEPLPVPVVGEKEDDVPQREDYFEPISPDRTSVHQEGQYSDEGEVEEDHHEEGEDEEDVDVEEEEDEDEEDGHTVESIPEEEEDEEEEEEGEEEEGEEEEDEEEEGEGEEEEEEEEGEGDDGYEQISSDEDGIADLERETFKYPNFDVEYTAEDLASVPPMTYDPYDRELVPLAYFSCPYKTTFEIEVSRMKDQGPDKENSGAVEASVKLVELLELYQEDRGPKWVTALEEVPGLIVKGLSYLQFKNAQQDYIGQLVDWTMQALNLQVALRQPIALNVRQLKAGTKLVSSLAECGPQGVMGLLQAGVISGLFELLFADHVSSSLKLNAFKALDSIISMTEGMEDFLRGRQDLHEKSGYQKLLELILLDQTVRVVTAGSAILQKCHFYEILSEIKRLGDQLAERSSIPNHSESEQDADPGLERTNPEYENEVEASLDIVHLLESSLITEGEIEKLINLLDEIFHLMETAPHTMVQPPVKSFPTIARITGPPERDDPYPVLFRYLHSHHFLELVTLLLSIPAISAHPAVLQAMRDVLKFLTQSQKGLLFFLSEHEATNLLIRALCHLLDQEEEEGLQADGVIDDTFALWLQYSTQTLQFITELFCHFQRCTNNEETDHSDLLGTLHNLYQITFNPVGRAAVGHVFSLEKNLQSLITLMEYYSKEALGDSKSKKSVAYNYACILVLLVVQSSSDVQMLEQHAAPLLKLCKADENNTKLQELGKWLEPLKNLRFEINCIPSLIEYVKQNIDNLMTPEGVGLPTALRVLCNIACPPPPVEGQQKDLKWNLAVIQLFSAEGMDTFIRVLQKLNNILTQPWRLHVNMGTTLHRVTTISMARCTLTLLKTMLTELLRGGSFEFKDMRVPSALVTLHMLLCSIPLSGRLDSDEQKIQNDIIDILLTFTQGVNEKLTISEETLANNTWSLMLKEVLSSVLRVPEGFFSGLILLSELLPLPLPMQTTQVIEPHDISVALNTRKLWSMHLHVQAKLLQEIVRSFSGTTCLPIQHMLRRICVQLCDLASPTALLIMRTVLDLIVEDMQSNSEDKEKQYTSQTTRLLALLDALASHKACKLAILHLINGTIKGDERYAEIFQELLALRSTGESVIHQQCVEYVMSILQSLCDQDIALILPSSSEGSLSELEQLSNSLPNKELMVLICDCLLETVANSENSYNCLLTCVRTMMFLAEHDYGFFHLKSSLRKHSNALHRLLKRIVSTFSKDTGELASSFLEFMRQILNSDTMGCCGDDSGLMEIDGSHSSRTLSLNAAELKQLLQSKEESPESLLLQLEKHILEHSKEDDNLDSLQDNIVGLRQMLESAGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFSPFQAEEIETDLDLVKVDLIELSEKCYSDFDLQAELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHIVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGLPPPKRPPKVSQKISSRGGFSGNRGGRGAFHSQNRFFTPPASKGNYSRREGTRGSSWSAQSTPRGTYNESRGGQSNFSRGPLPPLRPLSSAGYRPSPRDRASRGRGGLGPSWASANSGSGGSRGKFVSGGSGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_023496393.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023496393.1

ORF Insert Sequence:

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGATGGTG AGAAAGAAGA GCAGTTTAAT GGAAGTCCTC CAAGACCACA GCCCCGAGGA 
CCAAGGACTC CTCCAGGACC CCCTCCACCT GATGATGATG ATGATGATGA ACCTCTGCCA 
GTACCAGTAG TTGGTGAGAA AGAAGATGAT GTTCCTCAAC GAGAAGATTA CTTTGAGCCC 
ATTTCACCTG ATCGGACTTC TGTTCATCAA GAAGGGCAGT ATTCAGATGA AGGTGAAGTA 
GAAGAGGATC ATCATGAGGA AGGTGAAGAT GAAGAAGATG TAGATGTTGA AGAAGAGGAG 
GATGAAGATG AGGAAGATGG TCATACAGTA GAGAGTATCC CAGAAGAAGA AGAAGATGAA 
GAAGAGGAGG AAGAAGGTGA AGAAGAAGAA GGTGAAGAAG AGGAAGATGA AGAAGAAGAA 
GGTGAAGGAG AAGAAGAAGA AGAAGAAGAA GAAGGTGAAG GAGATGATGG CTATGAGCAA 
ATTTCTAGTG ATGAAGATGG AATTGCTGAT TTAGAACGTG AGACTTTTAA ATATCCGAAC 
TTTGATGTTG AATATACTGC TGAGGATTTA GCTTCAGTGC CTCCAATGAC ATATGATCCA 
TATGACAGAG AACTTGTACC ACTTGCATAC TTTAGCTGCC CTTACAAGAC AACTTTTGAA 
ATTGAAGTCA GCAGGATGAA AGACCAAGGT CCTGATAAAG AAAATTCAGG GGCAGTAGAA 
GCATCAGTAA AGTTAGTGGA ACTTTTAGAG TTATACCAGG AAGATAGAGG TCCAAAATGG 
GTCACAGCTT TGGAAGAAGT TCCAGGTTTA ATAGTAAAAG GATTAAGTTA TTTGCAGTTC 
AAAAATGCAC AACAAGACTA CATTGGCCAG CTGGTAGATT GGACCATGCA AGCCTTGAAT 
TTACAAGTAG CTCTCCGTCA GCCCATTGCC TTAAATGTCC GGCAGCTCAA AGCTGGGACT 
AAATTAGTAT CCTCATTAGC AGAATGTGGG CCTCAAGGAG TGATGGGACT TTTACAAGCA 
GGAGTTATCA GTGGATTATT TGAACTGCTA TTTGCTGACC ATGTATCATC TTCTCTTAAG 
CTAAATGCTT TTAAAGCTTT GGACAGTATT ATTAGTATGA CTGAAGGCAT GGAGGATTTT 
TTAAGAGGCA GACAGGACTT ACATGAAAAA AGTGGTTATC AGAAACTTCT GGAGCTCATT 
CTCTTGGATC AGACCGTGAG AGTAGTTACT GCTGGTTCTG CAATTCTCCA GAAATGTCAC 
TTCTATGAGA TTCTGTCTGA AATTAAAAGA CTTGGTGATC AGTTGGCCGA GAGGTCTTCT 
ATTCCTAACC ACAGCGAATC TGAGCAGGAC GCAGACCCTG GACTTGAGAG AACAAATCCT 
GAATATGAAA ATGAAGTGGA AGCTTCTTTG GATATAGTTC ATCTTTTGGA ATCCTCTCTT 
ATAACTGAAG GGGAAATAGA AAAGCTTATT AATCTCTTGG ATGAAATCTT CCACTTAATG 
GAGACTGCAC CTCACACGAT GGTTCAGCCT CCTGTGAAGT CATTCCCGAC AATAGCAAGG 
ATTACTGGAC CTCCAGAGAG AGATGATCCT TATCCTGTCT TATTCAGATA CCTCCACAGC 
CACCACTTCC TAGAATTGGT CACCTTACTG CTGTCAATTC CAGCAATAAG CGCTCACCCT 
GCTGTGCTGC AGGCTATGAG AGATGTTTTG AAGTTTCTTA CACAGTCGCA AAAAGGTCTT 
CTTTTTTTCC TCTCGGAACA TGAGGCGACT AACTTGCTGA TTAGAGCTCT TTGTCACCTT 
CTTGATCAAG AAGAAGAAGA AGGTCTCCAG GCTGATGGTG TTATTGATGA TACATTTGCT 
TTGTGGCTAC AGTATTCAAC TCAGACTTTG CAGTTTATTA CAGAACTATT TTGTCATTTT 
CAGCGTTGCA CAAACAATGA GGAAACTGAC CATTCAGATC TCTTGGGAAC CCTTCATAAT 
CTTTACCAGA TTACTTTTAA TCCTGTGGGA AGAGCAGCTG TTGGTCATGT CTTCAGTCTT 
GAAAAGAATC TCCAGAGTCT TATTACTCTA ATGGAGTACT ATTCCAAAGA GGCTTTAGGG 
GACTCCAAGT CAAAGAAATC GGTAGCTTAT AATTATGCGT GCATACTTGT TTTGTTGGTG 
GTTCAGTCTT CTAGTGATGT CCAGATGCTA GAGCAACATG CAGCGCCTCT CTTGAAGCTT 
TGTAAAGCAG ATGAAAATAA CACGAAATTG CAAGAACTTG GCAAGTGGCT GGAGCCTCTG 
AAAAACCTTA GATTTGAAAT TAATTGTATC CCAAGCCTAA TTGAATATGT TAAACAGAAT 
ATTGATAACT TGATGACCCC AGAAGGAGTT GGTCTACCAA CTGCATTACG TGTCCTCTGT 
AACATAGCGT GCCCACCACC TCCTGTTGAA GGTCAACAGA AAGATCTGAA GTGGAACCTT 
GCAGTTATTC AGCTTTTTTC TGCTGAAGGA ATGGACACCT TTATTCGTGT TCTGCAAAAA 
CTGAACAATA TTCTTACGCA GCCTTGGAGA CTCCATGTCA ACATGGGGAC TACTCTTCAT 
CGAGTTACTA CCATTTCCAT GGCCCGCTGC ACACTCACTC TTCTTAAAAC CATGTTAACA 
GAGCTTCTCA GGGGTGGATC CTTTGAGTTT AAAGACATGC GTGTTCCTTC AGCCCTTGTT 
ACTTTACACA TGCTACTGTG CTCTATCCCT CTCTCAGGTC GTCTGGATAG TGATGAGCAG 
AAGATTCAGA ATGACATCAT TGATATCTTA CTGACTTTTA CGCAGGGAGT TAATGAAAAA 
CTTACAATTT CGGAAGAGAC TTTAGCCAAT AATACTTGGT CTTTAATGTT AAAAGAAGTT 
CTTTCTTCAG TCTTGAGAGT TCCTGAAGGA TTTTTTTCTG GACTCATTCT GCTTTCAGAG 
CTCCTGCCCC TTCCATTGCC CATGCAAACA ACTCAGGTTA TTGAGCCACA TGATATATCA 
GTGGCACTCA ACACTCGCAA ACTGTGGAGT ATGCATCTCC ACGTCCAGGC AAAGCTGCTC 
CAAGAAATAG TTCGTTCTTT CTCTGGCACA ACTTGCCTGC CAATTCAGCA CATGTTGCGG 
CGCATTTGTG TTCAATTATG TGACCTTGCT TCACCAACTG CACTTCTAAT CATGAGAACT 
GTCTTGGATT TGATTGTAGA AGACATGCAA AGCAATTCAG AAGATAAAGA AAAACAGTAT 
ACAAGTCAAA CAACCAGACT TCTGGCTCTT CTTGATGCCC TGGCTTCACA CAAAGCTTGT 
AAATTGGCTA TTTTGCATCT GATAAATGGA ACTATTAAAG GTGATGAACG ATATGCAGAG 
ATTTTCCAGG AATTGTTAGC TCTTCGATCA ACTGGAGAGA GTGTTATTCA TCAACAGTGT 
GTTGAATATG TCATGTCCAT TTTGCAGTCT CTCTGTGATC AGGACATAGC ACTTATTTTG 
CCAAGTTCTT CAGAAGGATC ACTTTCAGAA CTGGAACAGT TGTCCAATTC TTTACCAAAC 
AAGGAATTGA TGGTCTTGAT CTGTGACTGT CTACTGGAAA CAGTAGCCAA TTCTGAGAAT 
AGTTACAACT GCTTACTGAC ATGTGTCAGA ACTATGATGT TTCTTGCAGA ACATGATTAT 
GGATTCTTTC ACTTAAAAAG TTCATTAAGA AAACACAGTA ATGCTTTGCA CCGATTATTG 
AAACGCATTG TTAGTACATT TAGTAAGGAT ACAGGAGAAC TTGCATCTTC TTTTTTGGAA 
TTCATGAGAC AAATACTCAA CTCTGACACA ATGGGATGTT GTGGAGATGA CAGTGGTCTC 
ATGGAAATAG ATGGATCTCA TTCATCAAGA ACATTGAGTC TTAATGCTGC AGAATTAAAA 
CAACTTTTAC AGAGCAAAGA AGAAAGTCCA GAAAGTTTGC TTCTTCAACT GGAGAAGCAT 
ATTTTGGAAC ATTCTAAAGA GGATGACAAC CTGGATTCTT TGCAGGACAA CATAGTTGGT 
CTTAGACAAA TGCTGGAATC TGCAGGTGAT CCTTTACCAC TTAGTGATCA AGATGTGGAA 
CCAGTACTTT CAGCTCCAGA ATCTCTTCAA AATCTCTTTA ATAATAGGAC TGCATATGTG 
CTAGCTGATG TCATGGATGA CCAGCTGAAA TCCATGTGGT TCTCTCCATT CCAGGCTGAA 
GAGATTGAAA CAGATTTAGA TTTGGTAAAA GTTGATCTAA TTGAGCTGTC TGAAAAATGT 
TATAGTGACT TTGATTTGCA AGCCGAATTA GAGCGTTCCT TTTTGTCAGA ACCATCATCT 
CCTGGGAGAA CAAAGACTAC AAAAGGATTT AAACTTGGCA AGCACAAACA TGAGACTTTT 
ATAACATCGA GTGGAAAATC TGAATACATT GAACCTGCCA AGAGAGCACA CATTGTGCCA 
CCTCCCAGAG GAAGAGGCCG GGGAGGATTT GGACAGGGCA TCAGACCCCA TGATATCTTT 
CGTCAGAGGA AACAGAACAC CAGTAGGCCA CCATCTATGC ACGTGGATGA TTTTGTTGCA 
GCTGAAAGCA AAGAAGTGGT CCCACAAGAT GGGTTACCAC CACCAAAAAG GCCACCCAAA 
GTATCACAGA AGATTTCTTC TCGTGGTGGA TTTTCAGGGA ACCGAGGAGG ACGAGGTGCT 
TTTCATAGTC AGAATCGGTT CTTCACTCCA CCTGCTTCAA AAGGAAATTA TAGTCGTCGT 
GAGGGAACAC GAGGTTCTAG CTGGAGTGCG CAGAGTACAC CACGAGGAAC CTATAATGAA 
AGTAGAGGAG GGCAGAGCAA TTTTAGCAGG GGGCCTCTTC CACCCCTTCG GCCACTCAGT 
TCTGCAGGAT ATCGTCCAAG TCCACGCGAC CGTGCTTCCA GAGGTCGGGG TGGACTTGGT 
CCTTCTTGGG CAAGCGCAAA TAGTGGCAGT GGAGGCTCAA GAGGAAAATT TGTTAGTGGA 
GGCAGCGGGA GAGGTCGTCA TGTACGGTCC TTCACACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VIRMA ( XM_012541722.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012397176.1 Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X1, mRNA.

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CloneID	OSa121922
Clone ID Related Accession (Same CDS sequence)	XM_012541722.2 , XM_012541722.1 , XM_012541722.3
Accession Version	XM_012541722.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-10
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein virilizer homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823376.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012541722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGCAGATG GTGAGAAAGA AGAGCAGTTT AATGGAAGTC CTCCAAGACC ACAGCCCCGA 
GGACCAAGGA CTCCTCCAGG ACCCCCTCCA CCTGATGATG ATGATGATGA TGAACCTCTG 
CCAGTACCAG TAGTTGGTGA GAAAGAAGAT GATGTTCCTC AACGAGAAGA TTACTTTGAG 
CCCATTTCAC CTGATCGGAC TTCTGTTCAT CAAGAAGGGC AGTATTCAGA TGAAGGTGAA 
GTAGAAGAGG ATCATCATGA GGAAGGTGAA GATGAAGAAG ATGTAGATGT TGAAGAAGAG 
GAGGATGAAG ATGAGGAAGA TGGTCATACA GTAGAGAGTA TCCCAGAAGA AGAAGAAGAT 
GAAGAAGAGG AGGAAGAAGG TGAAGAAGAA GAAGGTGAAG AAGAGGAAGA TGAAGAAGAA 
GAAGGTGAAG GAGAAGAAGA AGAAGAAGAA GAAGAAGGTG AAGGAGATGA TGGCTATGAG 
CAAATTTCTA GTGATGAAGA TGGAATTGCT GATTTAGAAC GTGAGACTTT TAAATATCCG 
AACTTTGATG TTGAATATAC TGCTGAGGAT TTAGCTTCAG TGCCTCCAAT GACATATGAT 
CCATATGACA GAGAACTTGT ACCACTTGCA TACTTTAGCT GCCCTTACAA GACAACTTTT 
GAAATTGAAG TCAGCAGGAT GAAAGACCAA GGTCCTGATA AAGAAAATTC AGGGGCAGTA 
GAAGCATCAG TAAAGTTAGT GGAACTTTTA GAGTTATACC AGGAAGATAG AGGTCCAAAA 
TGGGTCACAG CTTTGGAAGA AGTTCCAGGT TTAATAGTAA AAGGATTAAG TTATTTGCAG 
TTCAAAAATG CACAACAAGA CTACATTGGC CAGCTGGTAG ATTGGACCAT GCAAGCCTTG 
AATTTACAAG TAGCTCTCCG TCAGCCCATT GCCTTAAATG TCCGGCAGCT CAAAGCTGGG 
ACTAAATTAG TATCCTCATT AGCAGAATGT GGGCCTCAAG GAGTGATGGG ACTTTTACAA 
GCAGGAGTTA TCAGTGGATT ATTTGAACTG CTATTTGCTG ACCATGTATC ATCTTCTCTT 
AAGCTAAATG CTTTTAAAGC TTTGGACAGT ATTATTAGTA TGACTGAAGG CATGGAGGAT 
TTTTTAAGAG GCAGACAGGA CTTACATGAA AAAAGTGGTT ATCAGAAACT TCTGGAGCTC 
ATTCTCTTGG ATCAGACCGT GAGAGTAGTT ACTGCTGGTT CTGCAATTCT CCAGAAATGT 
CACTTCTATG AGATTCTGTC TGAAATTAAA AGACTTGGTG ATCAGTTGGC CGAGAGGTCT 
TCTATTCCTA ACCACAGCGA ATCTGAGCAG GACGCAGACC CTGGACTTGA GAGAACAAAT 
CCTGAATATG AAAATGAAGT GGAAGCTTCT TTGGATATAG TTCATCTTTT GGAATCCTCT 
CTTATAACTG AAGGGGAAAT AGAAAAGCTT ATTAATCTCT TGGATGAAAT CTTCCACTTA 
ATGGAGACTG CACCTCACAC GATGGTTCAG CCTCCTGTGA AGTCATTCCC GACAATAGCA 
AGGATTACTG GACCTCCAGA GAGAGATGAT CCTTATCCTG TCTTATTCAG ATACCTCCAC 
AGCCACCACT TCCTAGAATT GGTCACCTTA CTGCTGTCAA TTCCAGCAAT AAGCGCTCAC 
CCTGCTGTGC TGCAGGCTAT GAGAGATGTT TTGAAGTTTC TTACACAGTC GCAAAAAGGT 
CTTCTTTTTT TCCTCTCGGA ACATGAGGCG ACTAACTTGC TGATTAGAGC TCTTTGTCAC 
CTTCTTGATC AAGAAGAAGA AGAAGGTCTC CAGGCTGATG GTGTTATTGA TGATACATTT 
GCTTTGTGGC TACAGTATTC AACTCAGACT TTGCAGTTTA TTACAGAACT ATTTTGTCAT 
TTTCAGCGTT GCACAAACAA TGAGGAAACT GACCATTCAG ATCTCTTGGG AACCCTTCAT 
AATCTTTACC AGATTACTTT TAATCCTGTG GGAAGAGCAG CTGTTGGTCA TGTCTTCAGT 
CTTGAAAAGA ATCTCCAGAG TCTTATTACT CTAATGGAGT ACTATTCCAA AGAGGCTTTA 
GGGGACTCCA AGTCAAAGAA ATCGGTAGCT TATAATTATG CGTGCATACT TGTTTTGTTG 
GTGGTTCAGT CTTCTAGTGA TGTCCAGATG CTAGAGCAAC ATGCAGCGCC TCTCTTGAAG 
CTTTGTAAAG CAGATGAAAA TAACACGAAA TTGCAAGAAC TTGGCAAGTG GCTGGAGCCT 
CTGAAAAACC TTAGATTTGA AATTAATTGT ATCCCAAGCC TAATTGAATA TGTTAAACAG 
AATATTGATA ACTTGATGAC CCCAGAAGGA GTTGGTCTAC CAACTGCATT ACGTGTCCTC 
TGTAACATAG CGTGCCCACC ACCTCCTGTT GAAGGTCAAC AGAAAGATCT GAAGTGGAAC 
CTTGCAGTTA TTCAGCTTTT TTCTGCTGAA GGAATGGACA CCTTTATTCG TGTTCTGCAA 
AAACTGAACA ATATTCTTAC GCAGCCTTGG AGACTCCATG TCAACATGGG GACTACTCTT 
CATCGAGTTA CTACCATTTC CATGGCCCGC TGCACACTCA CTCTTCTTAA AACCATGTTA 
ACAGAGCTTC TCAGGGGTGG ATCCTTTGAG TTTAAAGACA TGCGTGTTCC TTCAGCCCTT 
GTTACTTTAC ACATGCTACT GTGCTCTATC CCTCTCTCAG GTCGTCTGGA TAGTGATGAG 
CAGAAGATTC AGAATGACAT CATTGATATC TTACTGACTT TTACGCAGGG AGTTAATGAA 
AAACTTACAA TTTCGGAAGA GACTTTAGCC AATAATACTT GGTCTTTAAT GTTAAAAGAA 
GTTCTTTCTT CAGTCTTGAG AGTTCCTGAA GGATTTTTTT CTGGACTCAT TCTGCTTTCA 
GAGCTCCTGC CCCTTCCATT GCCCATGCAA ACAACTCAGG TTATTGAGCC ACATGATATA 
TCAGTGGCAC TCAACACTCG CAAACTGTGG AGTATGCATC TCCACGTCCA GGCAAAGCTG 
CTCCAAGAAA TAGTTCGTTC TTTCTCTGGC ACAACTTGCC TGCCAATTCA GCACATGTTG 
CGGCGCATTT GTGTTCAATT ATGTGACCTT GCTTCACCAA CTGCACTTCT AATCATGAGA 
ACTGTCTTGG ATTTGATTGT AGAAGACATG CAAAGCAATT CAGAAGATAA AGAAAAACAG 
TATACAAGTC AAACAACCAG ACTTCTGGCT CTTCTTGATG CCCTGGCTTC ACACAAAGCT 
TGTAAATTGG CTATTTTGCA TCTGATAAAT GGAACTATTA AAGGTGATGA ACGATATGCA 
GAGATTTTCC AGGAATTGTT AGCTCTTCGA TCAACTGGAG AGAGTGTTAT TCATCAACAG 
TGTGTTGAAT ATGTCATGTC CATTTTGCAG TCTCTCTGTG ATCAGGACAT AGCACTTATT 
TTGCCAAGTT CTTCAGAAGG ATCACTTTCA GAACTGGAAC AGTTGTCCAA TTCTTTACCA 
AACAAGGAAT TGATGGTCTT GATCTGTGAC TGTCTACTGG AAACAGTAGC CAATTCTGAG 
AATAGTTACA ACTGCTTACT GACATGTGTC AGAACTATGA TGTTTCTTGC AGAACATGAT 
TATGGATTCT TTCACTTAAA AAGTTCATTA AGAAAACACA GTAATGCTTT GCACCGATTA 
TTGAAACGCA TTGTTAGTAC ATTTAGTAAG GATACAGGAG AACTTGCATC TTCTTTTTTG 
GAATTCATGA GACAAATACT CAACTCTGAC ACAATGGGAT GTTGTGGAGA TGACAGTGGT 
CTCATGGAAA TAGATGGATC TCATTCATCA AGAACATTGA GTCTTAATGC TGCAGAATTA 
AAACAACTTT TACAGAGCAA AGAAGAAAGT CCAGAAAGTT TGCTTCTTCA ACTGGAGAAG 
CATATTTTGG AACATTCTAA AGAGGATGAC AACCTGGATT CTTTGCAGGA CAACATAGTT 
GGTCTTAGAC AAATGCTGGA ATCTGCAGGT GATCCTTTAC CACTTAGTGA TCAAGATGTG 
GAACCAGTAC TTTCAGCTCC AGAATCTCTT CAAAATCTCT TTAATAATAG GACTGCATAT 
GTGCTAGCTG ATGTCATGGA TGACCAGCTG AAATCCATGT GGTTCTCTCC ATTCCAGGCT 
GAAGAGATTG AAACAGATTT AGATTTGGTA AAAGTTGATC TAATTGAGCT GTCTGAAAAA 
TGTTATAGTG ACTTTGATTT GCAAGCCGAA TTAGAGCGTT CCTTTTTGTC AGAACCATCA 
TCTCCTGGGA GAACAAAGAC TACAAAAGGA TTTAAACTTG GCAAGCACAA ACATGAGACT 
TTTATAACAT CGAGTGGAAA ATCTGAATAC ATTGAACCTG CCAAGAGAGC ACACATTGTG 
CCACCTCCCA GAGGAAGAGG CCGGGGAGGA TTTGGACAGG GCATCAGACC CCATGATATC 
TTTCGTCAGA GGAAACAGAA CACCAGTAGG CCACCATCTA TGCACGTGGA TGATTTTGTT 
GCAGCTGAAA GCAAAGAAGT GGTCCCACAA GATGGGTTAC CACCACCAAA AAGGCCACCC 
AAAGTATCAC AGAAGATTTC TTCTCGTGGT GGATTTTCAG GGAACCGAGG AGGACGAGGT 
GCTTTTCATA GTCAGAATCG GTTCTTCACT CCACCTGCTT CAAAAGGAAA TTATAGTCGT 
CGTGAGGGAA CACGAGGTTC TAGCTGGAGT GCGCAGAGTA CACCACGAGG AACCTATAAT 
GAAAGTAGAG GAGGGCAGAG CAATTTTAGC AGGGGGCCTC TTCCACCCCT TCGGCCACTC 
AGTTCTGCAG GATATCGTCC AAGTCCACGC GACCGTGCTT CCAGAGGTCG GGGTGGACTT 
GGTCCTTCTT GGGCAAGCGC AAATAGTGGC AGTGGAGGCT CAAGAGGAAA ATTTGTTAGT 
GGAGGCAGCG GGAGAGGTCG TCATGTACGG TCCTTCACAC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012397176.1
CDS	12..5516
Translation	MAVDSAMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSSLPDNRAYGETSPHTFQLDFFFNNVSKPSAPVFDRLGSLDYDENTSIIFRPNAKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQQQPSVKRTPKHADGEKEEQFNGSPPRPQPRGPRTPPGPPPPDDDDDDEPLPVPVVGEKEDDVPQREDYFEPISPDRTSVHQEGQYSDEGEVEEDHHEEGEDEEDVDVEEEEDEDEEDGHTVESIPEEEEDEEEEEEGEEEEGEEEEDEEEEGEGEEEEEEEEGEGDDGYEQISSDEDGIADLERETFKYPNFDVEYTAEDLASVPPMTYDPYDRELVPLAYFSCPYKTTFEIEVSRMKDQGPDKENSGAVEASVKLVELLELYQEDRGPKWVTALEEVPGLIVKGLSYLQFKNAQQDYIGQLVDWTMQALNLQVALRQPIALNVRQLKAGTKLVSSLAECGPQGVMGLLQAGVISGLFELLFADHVSSSLKLNAFKALDSIISMTEGMEDFLRGRQDLHEKSGYQKLLELILLDQTVRVVTAGSAILQKCHFYEILSEIKRLGDQLAERSSIPNHSESEQDADPGLERTNPEYENEVEASLDIVHLLESSLITEGEIEKLINLLDEIFHLMETAPHTMVQPPVKSFPTIARITGPPERDDPYPVLFRYLHSHHFLELVTLLLSIPAISAHPAVLQAMRDVLKFLTQSQKGLLFFLSEHEATNLLIRALCHLLDQEEEEGLQADGVIDDTFALWLQYSTQTLQFITELFCHFQRCTNNEETDHSDLLGTLHNLYQITFNPVGRAAVGHVFSLEKNLQSLITLMEYYSKEALGDSKSKKSVAYNYACILVLLVVQSSSDVQMLEQHAAPLLKLCKADENNTKLQELGKWLEPLKNLRFEINCIPSLIEYVKQNIDNLMTPEGVGLPTALRVLCNIACPPPPVEGQQKDLKWNLAVIQLFSAEGMDTFIRVLQKLNNILTQPWRLHVNMGTTLHRVTTISMARCTLTLLKTMLTELLRGGSFEFKDMRVPSALVTLHMLLCSIPLSGRLDSDEQKIQNDIIDILLTFTQGVNEKLTISEETLANNTWSLMLKEVLSSVLRVPEGFFSGLILLSELLPLPLPMQTTQVIEPHDISVALNTRKLWSMHLHVQAKLLQEIVRSFSGTTCLPIQHMLRRICVQLCDLASPTALLIMRTVLDLIVEDMQSNSEDKEKQYTSQTTRLLALLDALASHKACKLAILHLINGTIKGDERYAEIFQELLALRSTGESVIHQQCVEYVMSILQSLCDQDIALILPSSSEGSLSELEQLSNSLPNKELMVLICDCLLETVANSENSYNCLLTCVRTMMFLAEHDYGFFHLKSSLRKHSNALHRLLKRIVSTFSKDTGELASSFLEFMRQILNSDTMGCCGDDSGLMEIDGSHSSRTLSLNAAELKQLLQSKEESPESLLLQLEKHILEHSKEDDNLDSLQDNIVGLRQMLESAGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFSPFQAEEIETDLDLVKVDLIELSEKCYSDFDLQAELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHIVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGLPPPKRPPKVSQKISSRGGFSGNRGGRGAFHSQNRFFTPPASKGNYSRREGTRGSSWSAQSTPRGTYNESRGGQSNFSRGPLPPLRPLSSAGYRPSPRDRASRGRGGLGPSWASANSGSGGSRGKFVSGGSGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_012541722.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii vir like m6A methyltransferase associated (VIRMA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012541722.2

ORF Insert Sequence:

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGCAGATG GTGAGAAAGA AGAGCAGTTT AATGGAAGTC CTCCAAGACC ACAGCCCCGA 
GGACCAAGGA CTCCTCCAGG ACCCCCTCCA CCTGATGATG ATGATGATGA TGAACCTCTG 
CCAGTACCAG TAGTTGGTGA GAAAGAAGAT GATGTTCCTC AACGAGAAGA TTACTTTGAG 
CCCATTTCAC CTGATCGGAC TTCTGTTCAT CAAGAAGGGC AGTATTCAGA TGAAGGTGAA 
GTAGAAGAGG ATCATCATGA GGAAGGTGAA GATGAAGAAG ATGTAGATGT TGAAGAAGAG 
GAGGATGAAG ATGAGGAAGA TGGTCATACA GTAGAGAGTA TCCCAGAAGA AGAAGAAGAT 
GAAGAAGAGG AGGAAGAAGG TGAAGAAGAA GAAGGTGAAG AAGAGGAAGA TGAAGAAGAA 
GAAGGTGAAG GAGAAGAAGA AGAAGAAGAA GAAGAAGGTG AAGGAGATGA TGGCTATGAG 
CAAATTTCTA GTGATGAAGA TGGAATTGCT GATTTAGAAC GTGAGACTTT TAAATATCCG 
AACTTTGATG TTGAATATAC TGCTGAGGAT TTAGCTTCAG TGCCTCCAAT GACATATGAT 
CCATATGACA GAGAACTTGT ACCACTTGCA TACTTTAGCT GCCCTTACAA GACAACTTTT 
GAAATTGAAG TCAGCAGGAT GAAAGACCAA GGTCCTGATA AAGAAAATTC AGGGGCAGTA 
GAAGCATCAG TAAAGTTAGT GGAACTTTTA GAGTTATACC AGGAAGATAG AGGTCCAAAA 
TGGGTCACAG CTTTGGAAGA AGTTCCAGGT TTAATAGTAA AAGGATTAAG TTATTTGCAG 
TTCAAAAATG CACAACAAGA CTACATTGGC CAGCTGGTAG ATTGGACCAT GCAAGCCTTG 
AATTTACAAG TAGCTCTCCG TCAGCCCATT GCCTTAAATG TCCGGCAGCT CAAAGCTGGG 
ACTAAATTAG TATCCTCATT AGCAGAATGT GGGCCTCAAG GAGTGATGGG ACTTTTACAA 
GCAGGAGTTA TCAGTGGATT ATTTGAACTG CTATTTGCTG ACCATGTATC ATCTTCTCTT 
AAGCTAAATG CTTTTAAAGC TTTGGACAGT ATTATTAGTA TGACTGAAGG CATGGAGGAT 
TTTTTAAGAG GCAGACAGGA CTTACATGAA AAAAGTGGTT ATCAGAAACT TCTGGAGCTC 
ATTCTCTTGG ATCAGACCGT GAGAGTAGTT ACTGCTGGTT CTGCAATTCT CCAGAAATGT 
CACTTCTATG AGATTCTGTC TGAAATTAAA AGACTTGGTG ATCAGTTGGC CGAGAGGTCT 
TCTATTCCTA ACCACAGCGA ATCTGAGCAG GACGCAGACC CTGGACTTGA GAGAACAAAT 
CCTGAATATG AAAATGAAGT GGAAGCTTCT TTGGATATAG TTCATCTTTT GGAATCCTCT 
CTTATAACTG AAGGGGAAAT AGAAAAGCTT ATTAATCTCT TGGATGAAAT CTTCCACTTA 
ATGGAGACTG CACCTCACAC GATGGTTCAG CCTCCTGTGA AGTCATTCCC GACAATAGCA 
AGGATTACTG GACCTCCAGA GAGAGATGAT CCTTATCCTG TCTTATTCAG ATACCTCCAC 
AGCCACCACT TCCTAGAATT GGTCACCTTA CTGCTGTCAA TTCCAGCAAT AAGCGCTCAC 
CCTGCTGTGC TGCAGGCTAT GAGAGATGTT TTGAAGTTTC TTACACAGTC GCAAAAAGGT 
CTTCTTTTTT TCCTCTCGGA ACATGAGGCG ACTAACTTGC TGATTAGAGC TCTTTGTCAC 
CTTCTTGATC AAGAAGAAGA AGAAGGTCTC CAGGCTGATG GTGTTATTGA TGATACATTT 
GCTTTGTGGC TACAGTATTC AACTCAGACT TTGCAGTTTA TTACAGAACT ATTTTGTCAT 
TTTCAGCGTT GCACAAACAA TGAGGAAACT GACCATTCAG ATCTCTTGGG AACCCTTCAT 
AATCTTTACC AGATTACTTT TAATCCTGTG GGAAGAGCAG CTGTTGGTCA TGTCTTCAGT 
CTTGAAAAGA ATCTCCAGAG TCTTATTACT CTAATGGAGT ACTATTCCAA AGAGGCTTTA 
GGGGACTCCA AGTCAAAGAA ATCGGTAGCT TATAATTATG CGTGCATACT TGTTTTGTTG 
GTGGTTCAGT CTTCTAGTGA TGTCCAGATG CTAGAGCAAC ATGCAGCGCC TCTCTTGAAG 
CTTTGTAAAG CAGATGAAAA TAACACGAAA TTGCAAGAAC TTGGCAAGTG GCTGGAGCCT 
CTGAAAAACC TTAGATTTGA AATTAATTGT ATCCCAAGCC TAATTGAATA TGTTAAACAG 
AATATTGATA ACTTGATGAC CCCAGAAGGA GTTGGTCTAC CAACTGCATT ACGTGTCCTC 
TGTAACATAG CGTGCCCACC ACCTCCTGTT GAAGGTCAAC AGAAAGATCT GAAGTGGAAC 
CTTGCAGTTA TTCAGCTTTT TTCTGCTGAA GGAATGGACA CCTTTATTCG TGTTCTGCAA 
AAACTGAACA ATATTCTTAC GCAGCCTTGG AGACTCCATG TCAACATGGG GACTACTCTT 
CATCGAGTTA CTACCATTTC CATGGCCCGC TGCACACTCA CTCTTCTTAA AACCATGTTA 
ACAGAGCTTC TCAGGGGTGG ATCCTTTGAG TTTAAAGACA TGCGTGTTCC TTCAGCCCTT 
GTTACTTTAC ACATGCTACT GTGCTCTATC CCTCTCTCAG GTCGTCTGGA TAGTGATGAG 
CAGAAGATTC AGAATGACAT CATTGATATC TTACTGACTT TTACGCAGGG AGTTAATGAA 
AAACTTACAA TTTCGGAAGA GACTTTAGCC AATAATACTT GGTCTTTAAT GTTAAAAGAA 
GTTCTTTCTT CAGTCTTGAG AGTTCCTGAA GGATTTTTTT CTGGACTCAT TCTGCTTTCA 
GAGCTCCTGC CCCTTCCATT GCCCATGCAA ACAACTCAGG TTATTGAGCC ACATGATATA 
TCAGTGGCAC TCAACACTCG CAAACTGTGG AGTATGCATC TCCACGTCCA GGCAAAGCTG 
CTCCAAGAAA TAGTTCGTTC TTTCTCTGGC ACAACTTGCC TGCCAATTCA GCACATGTTG 
CGGCGCATTT GTGTTCAATT ATGTGACCTT GCTTCACCAA CTGCACTTCT AATCATGAGA 
ACTGTCTTGG ATTTGATTGT AGAAGACATG CAAAGCAATT CAGAAGATAA AGAAAAACAG 
TATACAAGTC AAACAACCAG ACTTCTGGCT CTTCTTGATG CCCTGGCTTC ACACAAAGCT 
TGTAAATTGG CTATTTTGCA TCTGATAAAT GGAACTATTA AAGGTGATGA ACGATATGCA 
GAGATTTTCC AGGAATTGTT AGCTCTTCGA TCAACTGGAG AGAGTGTTAT TCATCAACAG 
TGTGTTGAAT ATGTCATGTC CATTTTGCAG TCTCTCTGTG ATCAGGACAT AGCACTTATT 
TTGCCAAGTT CTTCAGAAGG ATCACTTTCA GAACTGGAAC AGTTGTCCAA TTCTTTACCA 
AACAAGGAAT TGATGGTCTT GATCTGTGAC TGTCTACTGG AAACAGTAGC CAATTCTGAG 
AATAGTTACA ACTGCTTACT GACATGTGTC AGAACTATGA TGTTTCTTGC AGAACATGAT 
TATGGATTCT TTCACTTAAA AAGTTCATTA AGAAAACACA GTAATGCTTT GCACCGATTA 
TTGAAACGCA TTGTTAGTAC ATTTAGTAAG GATACAGGAG AACTTGCATC TTCTTTTTTG 
GAATTCATGA GACAAATACT CAACTCTGAC ACAATGGGAT GTTGTGGAGA TGACAGTGGT 
CTCATGGAAA TAGATGGATC TCATTCATCA AGAACATTGA GTCTTAATGC TGCAGAATTA 
AAACAACTTT TACAGAGCAA AGAAGAAAGT CCAGAAAGTT TGCTTCTTCA ACTGGAGAAG 
CATATTTTGG AACATTCTAA AGAGGATGAC AACCTGGATT CTTTGCAGGA CAACATAGTT 
GGTCTTAGAC AAATGCTGGA ATCTGCAGGT GATCCTTTAC CACTTAGTGA TCAAGATGTG 
GAACCAGTAC TTTCAGCTCC AGAATCTCTT CAAAATCTCT TTAATAATAG GACTGCATAT 
GTGCTAGCTG ATGTCATGGA TGACCAGCTG AAATCCATGT GGTTCTCTCC ATTCCAGGCT 
GAAGAGATTG AAACAGATTT AGATTTGGTA AAAGTTGATC TAATTGAGCT GTCTGAAAAA 
TGTTATAGTG ACTTTGATTT GCAAGCCGAA TTAGAGCGTT CCTTTTTGTC AGAACCATCA 
TCTCCTGGGA GAACAAAGAC TACAAAAGGA TTTAAACTTG GCAAGCACAA ACATGAGACT 
TTTATAACAT CGAGTGGAAA ATCTGAATAC ATTGAACCTG CCAAGAGAGC ACACATTGTG 
CCACCTCCCA GAGGAAGAGG CCGGGGAGGA TTTGGACAGG GCATCAGACC CCATGATATC 
TTTCGTCAGA GGAAACAGAA CACCAGTAGG CCACCATCTA TGCACGTGGA TGATTTTGTT 
GCAGCTGAAA GCAAAGAAGT GGTCCCACAA GATGGGTTAC CACCACCAAA AAGGCCACCC 
AAAGTATCAC AGAAGATTTC TTCTCGTGGT GGATTTTCAG GGAACCGAGG AGGACGAGGT 
GCTTTTCATA GTCAGAATCG GTTCTTCACT CCACCTGCTT CAAAAGGAAA TTATAGTCGT 
CGTGAGGGAA CACGAGGTTC TAGCTGGAGT GCGCAGAGTA CACCACGAGG AACCTATAAT 
GAAAGTAGAG GAGGGCAGAG CAATTTTAGC AGGGGGCCTC TTCCACCCCT TCGGCCACTC 
AGTTCTGCAG GATATCGTCC AAGTCCACGC GACCGTGCTT CCAGAGGTCG GGGTGGACTT 
GGTCCTTCTT GGGCAAGCGC AAATAGTGGC AGTGGAGGCT CAAGAGGAAA ATTTGTTAGT 
GGAGGCAGCG GGAGAGGTCG TCATGTACGG TCCTTCACAC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VIRMA ( XM_012541722.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012397176.1 Sarcophilus harrisii KIAA1429 ortholog (KIAA1429), mRNA.

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CloneID	OSa121922
Clone ID Related Accession (Same CDS sequence)	XM_012541722.2 , XM_012541722.1 , XM_012541722.3
Accession Version	XM_012541722.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein virilizer homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823376.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGCAGATG GTGAGAAAGA AGAGCAGTTT AATGGAAGTC CTCCAAGACC ACAGCCCCGA 
GGACCAAGGA CTCCTCCAGG ACCCCCTCCA CCTGATGATG ATGATGATGA TGAACCTCTG 
CCAGTACCAG TAGTTGGTGA GAAAGAAGAT GATGTTCCTC AACGAGAAGA TTACTTTGAG 
CCCATTTCAC CTGATCGGAC TTCTGTTCAT CAAGAAGGGC AGTATTCAGA TGAAGGTGAA 
GTAGAAGAGG ATCATCATGA GGAAGGTGAA GATGAAGAAG ATGTAGATGT TGAAGAAGAG 
GAGGATGAAG ATGAGGAAGA TGGTCATACA GTAGAGAGTA TCCCAGAAGA AGAAGAAGAT 
GAAGAAGAGG AGGAAGAAGG TGAAGAAGAA GAAGGTGAAG AAGAGGAAGA TGAAGAAGAA 
GAAGGTGAAG GAGAAGAAGA AGAAGAAGAA GAAGAAGGTG AAGGAGATGA TGGCTATGAG 
CAAATTTCTA GTGATGAAGA TGGAATTGCT GATTTAGAAC GTGAGACTTT TAAATATCCG 
AACTTTGATG TTGAATATAC TGCTGAGGAT TTAGCTTCAG TGCCTCCAAT GACATATGAT 
CCATATGACA GAGAACTTGT ACCACTTGCA TACTTTAGCT GCCCTTACAA GACAACTTTT 
GAAATTGAAG TCAGCAGGAT GAAAGACCAA GGTCCTGATA AAGAAAATTC AGGGGCAGTA 
GAAGCATCAG TAAAGTTAGT GGAACTTTTA GAGTTATACC AGGAAGATAG AGGTCCAAAA 
TGGGTCACAG CTTTGGAAGA AGTTCCAGGT TTAATAGTAA AAGGATTAAG TTATTTGCAG 
TTCAAAAATG CACAACAAGA CTACATTGGC CAGCTGGTAG ATTGGACCAT GCAAGCCTTG 
AATTTACAAG TAGCTCTCCG TCAGCCCATT GCCTTAAATG TCCGGCAGCT CAAAGCTGGG 
ACTAAATTAG TATCCTCATT AGCAGAATGT GGGCCTCAAG GAGTGATGGG ACTTTTACAA 
GCAGGAGTTA TCAGTGGATT ATTTGAACTG CTATTTGCTG ACCATGTATC ATCTTCTCTT 
AAGCTAAATG CTTTTAAAGC TTTGGACAGT ATTATTAGTA TGACTGAAGG CATGGAGGAT 
TTTTTAAGAG GCAGACAGGA CTTACATGAA AAAAGTGGTT ATCAGAAACT TCTGGAGCTC 
ATTCTCTTGG ATCAGACCGT GAGAGTAGTT ACTGCTGGTT CTGCAATTCT CCAGAAATGT 
CACTTCTATG AGATTCTGTC TGAAATTAAA AGACTTGGTG ATCAGTTGGC CGAGAGGTCT 
TCTATTCCTA ACCACAGCGA ATCTGAGCAG GACGCAGACC CTGGACTTGA GAGAACAAAT 
CCTGAATATG AAAATGAAGT GGAAGCTTCT TTGGATATAG TTCATCTTTT GGAATCCTCT 
CTTATAACTG AAGGGGAAAT AGAAAAGCTT ATTAATCTCT TGGATGAAAT CTTCCACTTA 
ATGGAGACTG CACCTCACAC GATGGTTCAG CCTCCTGTGA AGTCATTCCC GACAATAGCA 
AGGATTACTG GACCTCCAGA GAGAGATGAT CCTTATCCTG TCTTATTCAG ATACCTCCAC 
AGCCACCACT TCCTAGAATT GGTCACCTTA CTGCTGTCAA TTCCAGCAAT AAGCGCTCAC 
CCTGCTGTGC TGCAGGCTAT GAGAGATGTT TTGAAGTTTC TTACACAGTC GCAAAAAGGT 
CTTCTTTTTT TCCTCTCGGA ACATGAGGCG ACTAACTTGC TGATTAGAGC TCTTTGTCAC 
CTTCTTGATC AAGAAGAAGA AGAAGGTCTC CAGGCTGATG GTGTTATTGA TGATACATTT 
GCTTTGTGGC TACAGTATTC AACTCAGACT TTGCAGTTTA TTACAGAACT ATTTTGTCAT 
TTTCAGCGTT GCACAAACAA TGAGGAAACT GACCATTCAG ATCTCTTGGG AACCCTTCAT 
AATCTTTACC AGATTACTTT TAATCCTGTG GGAAGAGCAG CTGTTGGTCA TGTCTTCAGT 
CTTGAAAAGA ATCTCCAGAG TCTTATTACT CTAATGGAGT ACTATTCCAA AGAGGCTTTA 
GGGGACTCCA AGTCAAAGAA ATCGGTAGCT TATAATTATG CGTGCATACT TGTTTTGTTG 
GTGGTTCAGT CTTCTAGTGA TGTCCAGATG CTAGAGCAAC ATGCAGCGCC TCTCTTGAAG 
CTTTGTAAAG CAGATGAAAA TAACACGAAA TTGCAAGAAC TTGGCAAGTG GCTGGAGCCT 
CTGAAAAACC TTAGATTTGA AATTAATTGT ATCCCAAGCC TAATTGAATA TGTTAAACAG 
AATATTGATA ACTTGATGAC CCCAGAAGGA GTTGGTCTAC CAACTGCATT ACGTGTCCTC 
TGTAACATAG CGTGCCCACC ACCTCCTGTT GAAGGTCAAC AGAAAGATCT GAAGTGGAAC 
CTTGCAGTTA TTCAGCTTTT TTCTGCTGAA GGAATGGACA CCTTTATTCG TGTTCTGCAA 
AAACTGAACA ATATTCTTAC GCAGCCTTGG AGACTCCATG TCAACATGGG GACTACTCTT 
CATCGAGTTA CTACCATTTC CATGGCCCGC TGCACACTCA CTCTTCTTAA AACCATGTTA 
ACAGAGCTTC TCAGGGGTGG ATCCTTTGAG TTTAAAGACA TGCGTGTTCC TTCAGCCCTT 
GTTACTTTAC ACATGCTACT GTGCTCTATC CCTCTCTCAG GTCGTCTGGA TAGTGATGAG 
CAGAAGATTC AGAATGACAT CATTGATATC TTACTGACTT TTACGCAGGG AGTTAATGAA 
AAACTTACAA TTTCGGAAGA GACTTTAGCC AATAATACTT GGTCTTTAAT GTTAAAAGAA 
GTTCTTTCTT CAGTCTTGAG AGTTCCTGAA GGATTTTTTT CTGGACTCAT TCTGCTTTCA 
GAGCTCCTGC CCCTTCCATT GCCCATGCAA ACAACTCAGG TTATTGAGCC ACATGATATA 
TCAGTGGCAC TCAACACTCG CAAACTGTGG AGTATGCATC TCCACGTCCA GGCAAAGCTG 
CTCCAAGAAA TAGTTCGTTC TTTCTCTGGC ACAACTTGCC TGCCAATTCA GCACATGTTG 
CGGCGCATTT GTGTTCAATT ATGTGACCTT GCTTCACCAA CTGCACTTCT AATCATGAGA 
ACTGTCTTGG ATTTGATTGT AGAAGACATG CAAAGCAATT CAGAAGATAA AGAAAAACAG 
TATACAAGTC AAACAACCAG ACTTCTGGCT CTTCTTGATG CCCTGGCTTC ACACAAAGCT 
TGTAAATTGG CTATTTTGCA TCTGATAAAT GGAACTATTA AAGGTGATGA ACGATATGCA 
GAGATTTTCC AGGAATTGTT AGCTCTTCGA TCAACTGGAG AGAGTGTTAT TCATCAACAG 
TGTGTTGAAT ATGTCATGTC CATTTTGCAG TCTCTCTGTG ATCAGGACAT AGCACTTATT 
TTGCCAAGTT CTTCAGAAGG ATCACTTTCA GAACTGGAAC AGTTGTCCAA TTCTTTACCA 
AACAAGGAAT TGATGGTCTT GATCTGTGAC TGTCTACTGG AAACAGTAGC CAATTCTGAG 
AATAGTTACA ACTGCTTACT GACATGTGTC AGAACTATGA TGTTTCTTGC AGAACATGAT 
TATGGATTCT TTCACTTAAA AAGTTCATTA AGAAAACACA GTAATGCTTT GCACCGATTA 
TTGAAACGCA TTGTTAGTAC ATTTAGTAAG GATACAGGAG AACTTGCATC TTCTTTTTTG 
GAATTCATGA GACAAATACT CAACTCTGAC ACAATGGGAT GTTGTGGAGA TGACAGTGGT 
CTCATGGAAA TAGATGGATC TCATTCATCA AGAACATTGA GTCTTAATGC TGCAGAATTA 
AAACAACTTT TACAGAGCAA AGAAGAAAGT CCAGAAAGTT TGCTTCTTCA ACTGGAGAAG 
CATATTTTGG AACATTCTAA AGAGGATGAC AACCTGGATT CTTTGCAGGA CAACATAGTT 
GGTCTTAGAC AAATGCTGGA ATCTGCAGGT GATCCTTTAC CACTTAGTGA TCAAGATGTG 
GAACCAGTAC TTTCAGCTCC AGAATCTCTT CAAAATCTCT TTAATAATAG GACTGCATAT 
GTGCTAGCTG ATGTCATGGA TGACCAGCTG AAATCCATGT GGTTCTCTCC ATTCCAGGCT 
GAAGAGATTG AAACAGATTT AGATTTGGTA AAAGTTGATC TAATTGAGCT GTCTGAAAAA 
TGTTATAGTG ACTTTGATTT GCAAGCCGAA TTAGAGCGTT CCTTTTTGTC AGAACCATCA 
TCTCCTGGGA GAACAAAGAC TACAAAAGGA TTTAAACTTG GCAAGCACAA ACATGAGACT 
TTTATAACAT CGAGTGGAAA ATCTGAATAC ATTGAACCTG CCAAGAGAGC ACACATTGTG 
CCACCTCCCA GAGGAAGAGG CCGGGGAGGA TTTGGACAGG GCATCAGACC CCATGATATC 
TTTCGTCAGA GGAAACAGAA CACCAGTAGG CCACCATCTA TGCACGTGGA TGATTTTGTT 
GCAGCTGAAA GCAAAGAAGT GGTCCCACAA GATGGGTTAC CACCACCAAA AAGGCCACCC 
AAAGTATCAC AGAAGATTTC TTCTCGTGGT GGATTTTCAG GGAACCGAGG AGGACGAGGT 
GCTTTTCATA GTCAGAATCG GTTCTTCACT CCACCTGCTT CAAAAGGAAA TTATAGTCGT 
CGTGAGGGAA CACGAGGTTC TAGCTGGAGT GCGCAGAGTA CACCACGAGG AACCTATAAT 
GAAAGTAGAG GAGGGCAGAG CAATTTTAGC AGGGGGCCTC TTCCACCCCT TCGGCCACTC 
AGTTCTGCAG GATATCGTCC AAGTCCACGC GACCGTGCTT CCAGAGGTCG GGGTGGACTT 
GGTCCTTCTT GGGCAAGCGC AAATAGTGGC AGTGGAGGCT CAAGAGGAAA ATTTGTTAGT 
GGAGGCAGCG GGAGAGGTCG TCATGTACGG TCCTTCACAC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012397176.1
CDS	12..5516
Translation	MAVDSAMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSSLPDNRAYGETSPHTFQLDFFFNNVSKPSAPVFDRLGSLDYDENTSIIFRPNAKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQQQPSVKRTPKHADGEKEEQFNGSPPRPQPRGPRTPPGPPPPDDDDDDEPLPVPVVGEKEDDVPQREDYFEPISPDRTSVHQEGQYSDEGEVEEDHHEEGEDEEDVDVEEEEDEDEEDGHTVESIPEEEEDEEEEEEGEEEEGEEEEDEEEEGEGEEEEEEEEGEGDDGYEQISSDEDGIADLERETFKYPNFDVEYTAEDLASVPPMTYDPYDRELVPLAYFSCPYKTTFEIEVSRMKDQGPDKENSGAVEASVKLVELLELYQEDRGPKWVTALEEVPGLIVKGLSYLQFKNAQQDYIGQLVDWTMQALNLQVALRQPIALNVRQLKAGTKLVSSLAECGPQGVMGLLQAGVISGLFELLFADHVSSSLKLNAFKALDSIISMTEGMEDFLRGRQDLHEKSGYQKLLELILLDQTVRVVTAGSAILQKCHFYEILSEIKRLGDQLAERSSIPNHSESEQDADPGLERTNPEYENEVEASLDIVHLLESSLITEGEIEKLINLLDEIFHLMETAPHTMVQPPVKSFPTIARITGPPERDDPYPVLFRYLHSHHFLELVTLLLSIPAISAHPAVLQAMRDVLKFLTQSQKGLLFFLSEHEATNLLIRALCHLLDQEEEEGLQADGVIDDTFALWLQYSTQTLQFITELFCHFQRCTNNEETDHSDLLGTLHNLYQITFNPVGRAAVGHVFSLEKNLQSLITLMEYYSKEALGDSKSKKSVAYNYACILVLLVVQSSSDVQMLEQHAAPLLKLCKADENNTKLQELGKWLEPLKNLRFEINCIPSLIEYVKQNIDNLMTPEGVGLPTALRVLCNIACPPPPVEGQQKDLKWNLAVIQLFSAEGMDTFIRVLQKLNNILTQPWRLHVNMGTTLHRVTTISMARCTLTLLKTMLTELLRGGSFEFKDMRVPSALVTLHMLLCSIPLSGRLDSDEQKIQNDIIDILLTFTQGVNEKLTISEETLANNTWSLMLKEVLSSVLRVPEGFFSGLILLSELLPLPLPMQTTQVIEPHDISVALNTRKLWSMHLHVQAKLLQEIVRSFSGTTCLPIQHMLRRICVQLCDLASPTALLIMRTVLDLIVEDMQSNSEDKEKQYTSQTTRLLALLDALASHKACKLAILHLINGTIKGDERYAEIFQELLALRSTGESVIHQQCVEYVMSILQSLCDQDIALILPSSSEGSLSELEQLSNSLPNKELMVLICDCLLETVANSENSYNCLLTCVRTMMFLAEHDYGFFHLKSSLRKHSNALHRLLKRIVSTFSKDTGELASSFLEFMRQILNSDTMGCCGDDSGLMEIDGSHSSRTLSLNAAELKQLLQSKEESPESLLLQLEKHILEHSKEDDNLDSLQDNIVGLRQMLESAGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFSPFQAEEIETDLDLVKVDLIELSEKCYSDFDLQAELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHIVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGLPPPKRPPKVSQKISSRGGFSGNRGGRGAFHSQNRFFTPPASKGNYSRREGTRGSSWSAQSTPRGTYNESRGGQSNFSRGPLPPLRPLSSAGYRPSPRDRASRGRGGLGPSWASANSGSGGSRGKFVSGGSGRGRHVRSFTR

Target ORF information:

RefSeq Version	XM_012541722.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii KIAA1429 ortholog (KIAA1429), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012541722.1

ORF Insert Sequence:

ATGGCGGTGG ACTCGGCGAT GGAGCTGCTA TTTTTAGACA CTTTCAAGCA CCCCAGCGCT 
GAGCAGAGTT CCCATATAGA TGTGGTTCGT TTTCCGTGTG TGGTTTATAT CAATGAAGTC 
CGTGTCATTC CACCAGGAGT AAGAGCTCAT AGTAGTCTGC CTGATAATAG AGCATATGGG 
GAAACCTCTC CGCATACATT TCAGCTAGAC TTTTTTTTCA ACAATGTAAG CAAACCAAGT 
GCTCCCGTTT TTGATAGGCT GGGAAGTCTG GACTATGATG AAAACACCTC TATCATCTTT 
AGACCTAATG CAAAGGTAAA TACTGATGGC TTGGTTCTAA GAGGTTGGTA CAACTGTCTG 
ACACTAGCAA TATATGGATC CGTTGATAGA GTGATAAGTC ATGATAGAGA CTCTCCACCA 
CCACCACCTC CACCTCCACC TCCTCCACAG CAGCAGCCAA GTGTAAAAAG AACTCCAAAA 
CATGCAGATG GTGAGAAAGA AGAGCAGTTT AATGGAAGTC CTCCAAGACC ACAGCCCCGA 
GGACCAAGGA CTCCTCCAGG ACCCCCTCCA CCTGATGATG ATGATGATGA TGAACCTCTG 
CCAGTACCAG TAGTTGGTGA GAAAGAAGAT GATGTTCCTC AACGAGAAGA TTACTTTGAG 
CCCATTTCAC CTGATCGGAC TTCTGTTCAT CAAGAAGGGC AGTATTCAGA TGAAGGTGAA 
GTAGAAGAGG ATCATCATGA GGAAGGTGAA GATGAAGAAG ATGTAGATGT TGAAGAAGAG 
GAGGATGAAG ATGAGGAAGA TGGTCATACA GTAGAGAGTA TCCCAGAAGA AGAAGAAGAT 
GAAGAAGAGG AGGAAGAAGG TGAAGAAGAA GAAGGTGAAG AAGAGGAAGA TGAAGAAGAA 
GAAGGTGAAG GAGAAGAAGA AGAAGAAGAA GAAGAAGGTG AAGGAGATGA TGGCTATGAG 
CAAATTTCTA GTGATGAAGA TGGAATTGCT GATTTAGAAC GTGAGACTTT TAAATATCCG 
AACTTTGATG TTGAATATAC TGCTGAGGAT TTAGCTTCAG TGCCTCCAAT GACATATGAT 
CCATATGACA GAGAACTTGT ACCACTTGCA TACTTTAGCT GCCCTTACAA GACAACTTTT 
GAAATTGAAG TCAGCAGGAT GAAAGACCAA GGTCCTGATA AAGAAAATTC AGGGGCAGTA 
GAAGCATCAG TAAAGTTAGT GGAACTTTTA GAGTTATACC AGGAAGATAG AGGTCCAAAA 
TGGGTCACAG CTTTGGAAGA AGTTCCAGGT TTAATAGTAA AAGGATTAAG TTATTTGCAG 
TTCAAAAATG CACAACAAGA CTACATTGGC CAGCTGGTAG ATTGGACCAT GCAAGCCTTG 
AATTTACAAG TAGCTCTCCG TCAGCCCATT GCCTTAAATG TCCGGCAGCT CAAAGCTGGG 
ACTAAATTAG TATCCTCATT AGCAGAATGT GGGCCTCAAG GAGTGATGGG ACTTTTACAA 
GCAGGAGTTA TCAGTGGATT ATTTGAACTG CTATTTGCTG ACCATGTATC ATCTTCTCTT 
AAGCTAAATG CTTTTAAAGC TTTGGACAGT ATTATTAGTA TGACTGAAGG CATGGAGGAT 
TTTTTAAGAG GCAGACAGGA CTTACATGAA AAAAGTGGTT ATCAGAAACT TCTGGAGCTC 
ATTCTCTTGG ATCAGACCGT GAGAGTAGTT ACTGCTGGTT CTGCAATTCT CCAGAAATGT 
CACTTCTATG AGATTCTGTC TGAAATTAAA AGACTTGGTG ATCAGTTGGC CGAGAGGTCT 
TCTATTCCTA ACCACAGCGA ATCTGAGCAG GACGCAGACC CTGGACTTGA GAGAACAAAT 
CCTGAATATG AAAATGAAGT GGAAGCTTCT TTGGATATAG TTCATCTTTT GGAATCCTCT 
CTTATAACTG AAGGGGAAAT AGAAAAGCTT ATTAATCTCT TGGATGAAAT CTTCCACTTA 
ATGGAGACTG CACCTCACAC GATGGTTCAG CCTCCTGTGA AGTCATTCCC GACAATAGCA 
AGGATTACTG GACCTCCAGA GAGAGATGAT CCTTATCCTG TCTTATTCAG ATACCTCCAC 
AGCCACCACT TCCTAGAATT GGTCACCTTA CTGCTGTCAA TTCCAGCAAT AAGCGCTCAC 
CCTGCTGTGC TGCAGGCTAT GAGAGATGTT TTGAAGTTTC TTACACAGTC GCAAAAAGGT 
CTTCTTTTTT TCCTCTCGGA ACATGAGGCG ACTAACTTGC TGATTAGAGC TCTTTGTCAC 
CTTCTTGATC AAGAAGAAGA AGAAGGTCTC CAGGCTGATG GTGTTATTGA TGATACATTT 
GCTTTGTGGC TACAGTATTC AACTCAGACT TTGCAGTTTA TTACAGAACT ATTTTGTCAT 
TTTCAGCGTT GCACAAACAA TGAGGAAACT GACCATTCAG ATCTCTTGGG AACCCTTCAT 
AATCTTTACC AGATTACTTT TAATCCTGTG GGAAGAGCAG CTGTTGGTCA TGTCTTCAGT 
CTTGAAAAGA ATCTCCAGAG TCTTATTACT CTAATGGAGT ACTATTCCAA AGAGGCTTTA 
GGGGACTCCA AGTCAAAGAA ATCGGTAGCT TATAATTATG CGTGCATACT TGTTTTGTTG 
GTGGTTCAGT CTTCTAGTGA TGTCCAGATG CTAGAGCAAC ATGCAGCGCC TCTCTTGAAG 
CTTTGTAAAG CAGATGAAAA TAACACGAAA TTGCAAGAAC TTGGCAAGTG GCTGGAGCCT 
CTGAAAAACC TTAGATTTGA AATTAATTGT ATCCCAAGCC TAATTGAATA TGTTAAACAG 
AATATTGATA ACTTGATGAC CCCAGAAGGA GTTGGTCTAC CAACTGCATT ACGTGTCCTC 
TGTAACATAG CGTGCCCACC ACCTCCTGTT GAAGGTCAAC AGAAAGATCT GAAGTGGAAC 
CTTGCAGTTA TTCAGCTTTT TTCTGCTGAA GGAATGGACA CCTTTATTCG TGTTCTGCAA 
AAACTGAACA ATATTCTTAC GCAGCCTTGG AGACTCCATG TCAACATGGG GACTACTCTT 
CATCGAGTTA CTACCATTTC CATGGCCCGC TGCACACTCA CTCTTCTTAA AACCATGTTA 
ACAGAGCTTC TCAGGGGTGG ATCCTTTGAG TTTAAAGACA TGCGTGTTCC TTCAGCCCTT 
GTTACTTTAC ACATGCTACT GTGCTCTATC CCTCTCTCAG GTCGTCTGGA TAGTGATGAG 
CAGAAGATTC AGAATGACAT CATTGATATC TTACTGACTT TTACGCAGGG AGTTAATGAA 
AAACTTACAA TTTCGGAAGA GACTTTAGCC AATAATACTT GGTCTTTAAT GTTAAAAGAA 
GTTCTTTCTT CAGTCTTGAG AGTTCCTGAA GGATTTTTTT CTGGACTCAT TCTGCTTTCA 
GAGCTCCTGC CCCTTCCATT GCCCATGCAA ACAACTCAGG TTATTGAGCC ACATGATATA 
TCAGTGGCAC TCAACACTCG CAAACTGTGG AGTATGCATC TCCACGTCCA GGCAAAGCTG 
CTCCAAGAAA TAGTTCGTTC TTTCTCTGGC ACAACTTGCC TGCCAATTCA GCACATGTTG 
CGGCGCATTT GTGTTCAATT ATGTGACCTT GCTTCACCAA CTGCACTTCT AATCATGAGA 
ACTGTCTTGG ATTTGATTGT AGAAGACATG CAAAGCAATT CAGAAGATAA AGAAAAACAG 
TATACAAGTC AAACAACCAG ACTTCTGGCT CTTCTTGATG CCCTGGCTTC ACACAAAGCT 
TGTAAATTGG CTATTTTGCA TCTGATAAAT GGAACTATTA AAGGTGATGA ACGATATGCA 
GAGATTTTCC AGGAATTGTT AGCTCTTCGA TCAACTGGAG AGAGTGTTAT TCATCAACAG 
TGTGTTGAAT ATGTCATGTC CATTTTGCAG TCTCTCTGTG ATCAGGACAT AGCACTTATT 
TTGCCAAGTT CTTCAGAAGG ATCACTTTCA GAACTGGAAC AGTTGTCCAA TTCTTTACCA 
AACAAGGAAT TGATGGTCTT GATCTGTGAC TGTCTACTGG AAACAGTAGC CAATTCTGAG 
AATAGTTACA ACTGCTTACT GACATGTGTC AGAACTATGA TGTTTCTTGC AGAACATGAT 
TATGGATTCT TTCACTTAAA AAGTTCATTA AGAAAACACA GTAATGCTTT GCACCGATTA 
TTGAAACGCA TTGTTAGTAC ATTTAGTAAG GATACAGGAG AACTTGCATC TTCTTTTTTG 
GAATTCATGA GACAAATACT CAACTCTGAC ACAATGGGAT GTTGTGGAGA TGACAGTGGT 
CTCATGGAAA TAGATGGATC TCATTCATCA AGAACATTGA GTCTTAATGC TGCAGAATTA 
AAACAACTTT TACAGAGCAA AGAAGAAAGT CCAGAAAGTT TGCTTCTTCA ACTGGAGAAG 
CATATTTTGG AACATTCTAA AGAGGATGAC AACCTGGATT CTTTGCAGGA CAACATAGTT 
GGTCTTAGAC AAATGCTGGA ATCTGCAGGT GATCCTTTAC CACTTAGTGA TCAAGATGTG 
GAACCAGTAC TTTCAGCTCC AGAATCTCTT CAAAATCTCT TTAATAATAG GACTGCATAT 
GTGCTAGCTG ATGTCATGGA TGACCAGCTG AAATCCATGT GGTTCTCTCC ATTCCAGGCT 
GAAGAGATTG AAACAGATTT AGATTTGGTA AAAGTTGATC TAATTGAGCT GTCTGAAAAA 
TGTTATAGTG ACTTTGATTT GCAAGCCGAA TTAGAGCGTT CCTTTTTGTC AGAACCATCA 
TCTCCTGGGA GAACAAAGAC TACAAAAGGA TTTAAACTTG GCAAGCACAA ACATGAGACT 
TTTATAACAT CGAGTGGAAA ATCTGAATAC ATTGAACCTG CCAAGAGAGC ACACATTGTG 
CCACCTCCCA GAGGAAGAGG CCGGGGAGGA TTTGGACAGG GCATCAGACC CCATGATATC 
TTTCGTCAGA GGAAACAGAA CACCAGTAGG CCACCATCTA TGCACGTGGA TGATTTTGTT 
GCAGCTGAAA GCAAAGAAGT GGTCCCACAA GATGGGTTAC CACCACCAAA AAGGCCACCC 
AAAGTATCAC AGAAGATTTC TTCTCGTGGT GGATTTTCAG GGAACCGAGG AGGACGAGGT 
GCTTTTCATA GTCAGAATCG GTTCTTCACT CCACCTGCTT CAAAAGGAAA TTATAGTCGT 
CGTGAGGGAA CACGAGGTTC TAGCTGGAGT GCGCAGAGTA CACCACGAGG AACCTATAAT 
GAAAGTAGAG GAGGGCAGAG CAATTTTAGC AGGGGGCCTC TTCCACCCCT TCGGCCACTC 
AGTTCTGCAG GATATCGTCC AAGTCCACGC GACCGTGCTT CCAGAGGTCG GGGTGGACTT 
GGTCCTTCTT GGGCAAGCGC AAATAGTGGC AGTGGAGGCT CAAGAGGAAA ATTTGTTAGT 
GGAGGCAGCG GGAGAGGTCG TCATGTACGG TCCTTCACAC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

VIRMA cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム