SHE2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SHE2
Entrez Gene ID	853728
Full Name	She2p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179696.1	NP_012792.1	She2p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AEL307C	NP_984553.1
Saccharomyces cerevisiae (baker's yeast)	SHE2	NP_012792.1
Kluyveromyces lactis	KLLA0B12518g	XP_452088.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Combining RNA- and membrane-binding features makes She2p an ideal coordinator of ER tubule and mRNA cotransport.
Title: Association of the yeast RNA-binding protein She2p with the tubular endoplasmic reticulum depends on membrane curvature.

The binding site for She3p was mapped to a single alpha helix that protrudes at right angles from She2p.
Title: Two single-headed myosin V motors bound to a tetrameric adapter protein form a processive complex.

She2p is recruited cotranscriptionally to the nascent bud-localized ASH1, IST2, and EAR1 mRNA
Title: Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud.

finding on She2p tetramerization resolves previously raised questions on complex formation and mRNP function.
Title: Formation of She2p tetramers is required for mRNA binding, mRNP assembly, and localization.

She2p interacts with both Loc1p and Puf6p and excluding She2p from the nucleus decreases this interaction.
Title: Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p.

The following SHE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02937	NM_001179696.1 Latest version!	Saccharomyces cerevisiae S288C She2p (SHE2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHE2 ( NM_001179696.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012792.1 Saccharomyces cerevisiae S288C She2p (SHE2), partial mRNA.

Price & Availability↑

CloneID	OSi02937
Clone ID Related Accession (Same CDS sequence)	NM_001179696.1
Accession Version	NM_001179696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	She2p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGCAAAG ACAAAGATAT CAAAGTCACT CCTGGAACTT GTGAATTAGT TGAACAAATA 
CTCGCCTTAC TCTCCCGGTA TCTCTCATCT TATATTCACG TGCTGAACAA GTTCATCAGT 
CATTTGCGAA GGGTAGCTAC GCTACGATTC GAGAGAACTA CTTTGATTAA ATTTGTTAAG 
AAATTGAGAT TTTACAACGA TTGTGTGTTA AGCTATAATG CATCAGAGTT TATCAATGAA 
GGTAAAAACG AATTAGACCC AGAGGCGGAT TCGTTTGACA AGGTAATTTT ACCAATAGCT 
TCAATGTTTG TTAAATGTGT TGAAACGTTT GACTTACTGA ACTATTATCT AACGCAGTCA 
TTACAAAAGG AAATTTTATC TAAAACTTTG AACGAGGACC TAACGCTAAC AGCAGAGTCC 
ATATTAGCCA TTGACGATAC CTACAATCAT TTCGTGAAAT TTTCTCAATG GATGATTGAG 
TCTTTGCGTA TAGGCAGCAA CCTGCTAGAT TTAGAAGTTG TTCAATTTGC CATTAAATGC 
GCAGATGAGG ACGGAACGAA TATCGGAGAA ACTGATAATA TCTTCTTGCA GGAGATTCTT 
CCTGTCAACT CCGAAGAAGA ATTTCAGACC TTGTCAGCTG CGTGGCACTC CATTTTAGAT 
GGTAAGCTGA GCGCCTTGGA CGAAGAATTT GATGTTGTCG CTACTAAATG GCATGACAAA 
TTTGGTAAAT TGAAAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012792.1
CDS	1..741
Translation	MSKDKDIKVTPGTCELVEQILALLSRYLSSYIHVLNKFISHLRRVATLRFERTTLIKFVKKLRFYNDCVLSYNASEFINEGKNELDPEADSFDKVILPIASMFVKCVETFDLLNYYLTQSLQKEILSKTLNEDLTLTAESILAIDDTYNHFVKFSQWMIESLRIGSNLLDLEVVQFAIKCADEDGTNIGETDNIFLQEILPVNSEEEFQTLSAAWHSILDGKLSALDEEFDVVATKWHDKFGKLKN

Target ORF information:

RefSeq Version	NM_001179696.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C She2p (SHE2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179696.1

ORF Insert Sequence:

ATGAGCAAAG ACAAAGATAT CAAAGTCACT CCTGGAACTT GTGAATTAGT TGAACAAATA 
CTCGCCTTAC TCTCCCGGTA TCTCTCATCT TATATTCACG TGCTGAACAA GTTCATCAGT 
CATTTGCGAA GGGTAGCTAC GCTACGATTC GAGAGAACTA CTTTGATTAA ATTTGTTAAG 
AAATTGAGAT TTTACAACGA TTGTGTGTTA AGCTATAATG CATCAGAGTT TATCAATGAA 
GGTAAAAACG AATTAGACCC AGAGGCGGAT TCGTTTGACA AGGTAATTTT ACCAATAGCT 
TCAATGTTTG TTAAATGTGT TGAAACGTTT GACTTACTGA ACTATTATCT AACGCAGTCA 
TTACAAAAGG AAATTTTATC TAAAACTTTG AACGAGGACC TAACGCTAAC AGCAGAGTCC 
ATATTAGCCA TTGACGATAC CTACAATCAT TTCGTGAAAT TTTCTCAATG GATGATTGAG 
TCTTTGCGTA TAGGCAGCAA CCTGCTAGAT TTAGAAGTTG TTCAATTTGC CATTAAATGC 
GCAGATGAGG ACGGAACGAA TATCGGAGAA ACTGATAATA TCTTCTTGCA GGAGATTCTT 
CCTGTCAACT CCGAAGAAGA ATTTCAGACC TTGTCAGCTG CGTGGCACTC CATTTTAGAT 
GGTAAGCTGA GCGCCTTGGA CGAAGAATTT GATGTTGTCG CTACTAAATG GCATGACAAA 
TTTGGTAAAT TGAAAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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SHE2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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