ORF » Species Summary » Saccharomyces cerevisiae S288C » TIM54 cDNA ORF clone

TIM54 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following TIM54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIM54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02656 NM_001181487.3
Latest version!		 Saccharomyces cerevisiae S288C Tim54p (TIM54), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    TIM54 ( NM_001181487.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012481.3 Saccharomyces cerevisiae S288C Tim54p (TIM54), partial mRNA.
    Price & Availability↑
    CloneID OSi02656
    Clone ID Related Accession (Same CDS sequence) NM_001181487.3
    Accession Version NM_001181487.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Tim54p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). On Jul 30, 2012 this sequence version replaced NM_001181487.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCTTCTG AATCAGGAAA GCCTATTGCT AAGCCTATTA GGAAGCCTGG CTATACCAAT 
    CCGGCTTTAA AAGCCCTTGG TATACCCGCC TTGAGGCTGC CTTCTCGAAA CTGGATGATC 
    TTTTGGTCTG TGTTAACAGT ATCCATCGGT GGCATAGCCT ATGACAAATA CAAGCAACGT 
    CAGATTCTGA GCCATGCAAC AGACTTGGTG AAGCCTTTGG CAGAGGAGTC CATGGAGGTA 
    GACAAAGTGC CACGAAAGAT CACTGTTTTT ATTGCACCTC CTCCAAATGA TTATCTGGAG 
    AGTTCGTTGA AAGTTTGGAG ACGATATGTT AAGCCTGTAC TGTATTATGC AGGGTTGGAC 
    TATGAATTAG TTCAAGAGGA TAGGCAAGGG ATTATTAGAA CTAATGTAGC CAACAGAATC 
    AGAGAATTGA GAAAGGAAAT ATTGGCATCG ACGGATGGGC AGCCAGTTAA AGAGCCTAAC 
    CAAACGGTAG CAAAACCAAG TGGTTCTTCA ACCAGTAAAA TTTCATCACT ACTACCGTTC 
    AATAAAATCA TTCAAGATCC TGCTGAAGAA GATGACTCGT TTGATCCAGA AATCGGTAAA 
    AAGTTCAAGG AGAACTTCGA CTGGAGAAAC GTTATAGGTA TCTTCTATAC AATGCCAAAA 
    CCGAAGCATA TAATAAGTGA AGACGCCCTG ACAAAGGACC CTATATTATC TGGTGGTGTT 
    ATTTGTTTAG GTAGAGGCGC CTATAAGGAG TACATTGCCG GTATTCACGA AGGCTTGCTT 
    GGACCCATCG AGAAGACCGA AAAAACAGGA TCCACTGAGC CTAAGATGAC TGGGGTTGTA 
    GAAGCAAATC AAATAGAATC AAAGGTAAGT GAATCCGGTG CTACGGAACT GGTGGATGCC 
    GAGAAAGAGA CTGCTTTGGA AGAGGCCAAA GTACAAGATG ATCTAAAGGT TGATGAAGAA 
    AACAGCTCAG AGGATTCGCA AAAATTTTTG AAGCCATTTA TTTCTTCAGA CCAATACCCT 
    GACCTGCAAA TCGCTTCGGA ATTACAAACT CCAAATGGCG AATTCATAAG AAACCCCAAT 
    ACAAATATCC CGCTTCTGAT TAATCAGCCG TTATTGGTCA TTCCAATACC TAACTTGATA 
    GGTTTTACTA CCATTCCAAG GAGAATACAT CGTTTTTACC AGAAGCGTTT CTACGTAGAG 
    GACGTGTGTT CAAGTGTAGT TAACTGTGTT CGACAAACCC GTATAAGACC GTTTGATATT 
    GCAAAGGATA TTGATTTGGC GAAGGACGAA GAAAAAGATT GGCCACAGAA CTGGGTCAAA 
    CAGGGTAAAG AAAAGAACAG CGAATGGACC CAAGAATTAG TATGTGATCC TAGAATCACG 
    AAGCATATGT TTGTTTACGA AAAACCACCC AAGGAAGAGC CAGAATCAGA TATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_012481.3
    CDS1..1437
    Translation

    Target ORF information:

    RefSeq Version NM_001181487.3
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Tim54p (TIM54), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001181487.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCTTCTG AATCAGGAAA GCCTATTGCT AAGCCTATTA GGAAGCCTGG CTATACCAAT 
    CCGGCTTTAA AAGCCCTTGG TATACCCGCC TTGAGGCTGC CTTCTCGAAA CTGGATGATC 
    TTTTGGTCTG TGTTAACAGT ATCCATCGGT GGCATAGCCT ATGACAAATA CAAGCAACGT 
    CAGATTCTGA GCCATGCAAC AGACTTGGTG AAGCCTTTGG CAGAGGAGTC CATGGAGGTA 
    GACAAAGTGC CACGAAAGAT CACTGTTTTT ATTGCACCTC CTCCAAATGA TTATCTGGAG 
    AGTTCGTTGA AAGTTTGGAG ACGATATGTT AAGCCTGTAC TGTATTATGC AGGGTTGGAC 
    TATGAATTAG TTCAAGAGGA TAGGCAAGGG ATTATTAGAA CTAATGTAGC CAACAGAATC 
    AGAGAATTGA GAAAGGAAAT ATTGGCATCG ACGGATGGGC AGCCAGTTAA AGAGCCTAAC 
    CAAACGGTAG CAAAACCAAG TGGTTCTTCA ACCAGTAAAA TTTCATCACT ACTACCGTTC 
    AATAAAATCA TTCAAGATCC TGCTGAAGAA GATGACTCGT TTGATCCAGA AATCGGTAAA 
    AAGTTCAAGG AGAACTTCGA CTGGAGAAAC GTTATAGGTA TCTTCTATAC AATGCCAAAA 
    CCGAAGCATA TAATAAGTGA AGACGCCCTG ACAAAGGACC CTATATTATC TGGTGGTGTT 
    ATTTGTTTAG GTAGAGGCGC CTATAAGGAG TACATTGCCG GTATTCACGA AGGCTTGCTT 
    GGACCCATCG AGAAGACCGA AAAAACAGGA TCCACTGAGC CTAAGATGAC TGGGGTTGTA 
    GAAGCAAATC AAATAGAATC AAAGGTAAGT GAATCCGGTG CTACGGAACT GGTGGATGCC 
    GAGAAAGAGA CTGCTTTGGA AGAGGCCAAA GTACAAGATG ATCTAAAGGT TGATGAAGAA 
    AACAGCTCAG AGGATTCGCA AAAATTTTTG AAGCCATTTA TTTCTTCAGA CCAATACCCT 
    GACCTGCAAA TCGCTTCGGA ATTACAAACT CCAAATGGCG AATTCATAAG AAACCCCAAT 
    ACAAATATCC CGCTTCTGAT TAATCAGCCG TTATTGGTCA TTCCAATACC TAACTTGATA 
    GGTTTTACTA CCATTCCAAG GAGAATACAT CGTTTTTACC AGAAGCGTTT CTACGTAGAG 
    GACGTGTGTT CAAGTGTAGT TAACTGTGTT CGACAAACCC GTATAAGACC GTTTGATATT 
    GCAAAGGATA TTGATTTGGC GAAGGACGAA GAAAAAGATT GGCCACAGAA CTGGGTCAAA 
    CAGGGTAAAG AAAAGAACAG CGAATGGACC CAAGAATTAG TATGTGATCC TAGAATCACG 
    AAGCATATGT TTGTTTACGA AAAACCACCC AAGGAAGAGC CAGAATCAGA TATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
    The EMBO journal15(9)2031-49(1996 May)
    Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L


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