CCH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CCH1
Entrez Gene ID	853131
Full Name	Cch1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181346.3	NP_011733.3	Cch1p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ACL143C	NP_983261.1
Schizosaccharomyces pombe (fission yeast)	cch1	NP_593894.1
Saccharomyces cerevisiae (baker's yeast)	CCH1	NP_011733.3
Kluyveromyces lactis	KLLA0E16083g	XP_454673.1
Neurospora crassa	NCU02762	XP_963732.2
Magnaporthe oryzae (rice blast fungus)	MGG_05643	XP_003710534.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The deletion of SCS2 did not affect Ca2+ influx activity when calcineurin was inhibited by FK506, but enhanced this activity by 35% when the enzyme was not inhibited. However, this enhancement was canceled by the deletion of ECM7. These results suggest that Cch1/Mid1 is regulated differentially by Ecm7 and Scs2 in a manner that is dependent on the phosphorylation status of Cch1.
Title: Genetic analysis of the regulation of the voltage-gated calcium channel homolog Cch1 by the ? subunit homolog Ecm7 and cortical ER protein Scs2 in yeast.

Therefore, H(+) extrusion by Pma1 may be important for Ca(2+) influx through Cch1/Mid1. These results suggest that Pma1 interacts physically with Cch1/Mid1 Ca(2+) channels to enhance their activity via its H(+) -pumping activity
Title: Coupling of a voltage-gated Ca

N1066D mutation in the cytoplasmic loop between domains II and III increased the activity of Cch1.
Title: Hyperactive and hypoactive mutations in Cch1, a yeast homologue of the voltage-gated calcium-channel pore-forming subunit.

Exposure to ethanol causes a dramatic calcium influx which is dependent on the Cch1 calcium channel.
Title: Ethanol induces calcium influx via the Cch1-Mid1 transporter in Saccharomyces cerevisiae.

These results suggest that Cch1 is pharmacologically similar to L-type VGCCs, but the interactions between Cch1 and the L-type VGCC blockers are more complicated than expected.
Title: Ion-channel blocker sensitivity of voltage-gated calcium-channel homologue Cch1 in Saccharomyces cerevisiae.

The following CCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02435	NM_001181346.3 Latest version!	Saccharomyces cerevisiae S288C Cch1p (CCH1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCH1 ( NM_001181346.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011733.3 Saccharomyces cerevisiae S288C Cch1p (CCH1), partial mRNA.

Price & Availability↑

CloneID	OSi02435
Clone ID Related Accession (Same CDS sequence)	NM_001181346.3
Accession Version	NM_001181346.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Cch1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181346.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCAGGGGA GAAAAAGGAC GCTTACGGAA CCATTTGAGC CAAATACCAA TCCATTTGGG 
GACAATGCAG CAGTAATGAC GGAAAATGTT GAGGATAACA GCGAAACAGA TGGTAACCGT 
CTAGAATCAA AACCACAAGC TTTGGTCCCA CCAGCTTTAA ATATCGTGCC ACCAGAGAGC 
AGCATCCACA GTACTGAAGA AAAAAAAGGT GACGAGTACA ATGGAAATGA TAAAGATAGC 
TCCTTAATCT CCAACATATT TCGTACTCGT GTCGGAAGGA GTAGTCATGA AAACTTGAGC 
AGGCCTAAAC TCTCACTTAA AACAGCATCA TTTGGTGCCG CTGAATCTTC CCGGCGTAAT 
GTTTCACCCT CTACAAAATC TGCCAAGTCT AGTTCGCAGT ATATTGATTT AAATGATGAA 
AGGCTACGCA GGCGTAGCTT CAGTAGTTAT AGCCGATCAT CTAGTAGGCG TGTTTCTAAT 
TCACCAAGCT CAACGGATAG GCCTCCACGG TCGGCAAAGG TTTTATCGCT AATTGCCGCT 
GATGATATGG ATGATTTTGA AGATTTGCAA AAGGGATTTA AAAGTGCAAT AGACGAAGAG 
GGCCTGACAT GGCTACCCCA ATTAAAATCA GAAAAAAGCC GTCCTGTATC AGACGTTGGA 
GAAGATAGAG GAGAAGGAGA ACAAGAATCT ATACCTGACG TTCATACACC CAATGTAGGA 
GCAAGCGCTA CTCCAGGATC AATTCATCTA ACACCCGAAC CCGCGCAGAA TGGTTCGGTA 
TCTGAGGGTC TAGAAGGCTC TATTAATAAT TCAAGAAAGA AACCCAGTCC AAAGTTTTTT 
CATCATTTAT CACCGCAAAA AGAAGATAAA GACCAAACAG AAGTTATTGA GTATGCTGAA 
GACATTCTAG ATTTTGAAAC CCTTCAAAGA AAACTGGAAT CAAGGCCCTT TGTGCTTTAT 
GGACATTCTC TTGGGGTTTT CTCGCCTACG AATCCGCTAA GAATAAAAAT TGCTCGTTTT 
TTGTTGCATA GGCGGTATTC GTTACTTTAC AACACTTTGT TAACATTTTA TGCCATTCTC 
CTGGCGATAA GGACATATAA CCCTCACAAT GTGGTTTTTT TATACCGTTT CTCTAACTGG 
ACCGACTATT TCATTTTTAT TTTATCAGCT TGCTTTACAG GCAATGATAT TGCTAAAATA 
ATTGCGTTTG GATTTTGGGA CGATTCTGAA ATGTTTAAAG CCTATGGACG TGAGTATAAA 
TCAATCTTAC AGAGATCTGG AATTATGAAA CTATACATAT ATCTGAGAGA AAAGTATGGT 
AGAAAGCTAA TAGATTTCAT TATTCCATTT AGGATCATAT CGCCGGGAGA AGAGACAAAA 
TATCAACGAA GTTCGTTGAG TACTTCCCTG ACGAAACCTT ATGGGGCAAA GGAAAATCAG 
AGGCCTTTTG GCACCCCAAG AGCCTTTGCG AGATCATCGT GGAATAGAAT AGATCTGGTA 
TCTTCTGTCA GTTTTTGGCT AGGTATGTTT TTATCCATAA AAAGTTATGA TACAAAAACA 
GGCATAAGAA TATTCAAGCC CCTTGCTATA TTAAGGATTC TTCGACTTGT AAACGTGGAT 
ACTGGTATGC CCTCAATTCT AAGGGGATTG AAATATGGTA TCCCACAGTT GGTAAATGTT 
AGTTCAATGC TAGTTTACTT TTGGATTTTC TTTGGGATTT TGGGAGTACA GATTTTTCAG 
GGCTCTTTTC GAAGACAATG TGTATGGTTT AACCCTGAAG ATCCTACCGA TACTTACCAG 
TACGATATGC AATTCTGTGG TGGTTACCTA GATCCAGTAA CAAAACGAAA ACAAAATTAT 
ATCTATGAAG ACGGATCAGA AGGTTCTGTT TCAAAAGGCT TTCTTTGCCC ACAATATTCG 
AAATGTGTTT CCAATGCCAA CCCCTACAAC GGCAGAATTA GTTTTGATAA TATTGTTAAT 
TCCATGGAGC TCGTTTTTGT CATTATGAGT GCGAATACTT TTACTGACTT AATGTACTAT 
ACGATGGATT CGGATGAAAT GGCTGCCTGT TTGTTTTTTA TTGTTTGCAT CTTTGTTTTA 
ACTATCTGGC TGCTAAATCT ACTCATTGCA GTGTTGGTAT CATCTTTTGA AATAGCCAAT 
GAGGAGTACA AAAAGAAAAA GTTCATATAT GGTTCTAGAA AGACAGGTTA TGTTGCTCGC 
ATAGTAACAG GCTACTGGAA ATATTTTAAG CTTAAGGCGA ATCAGACAAA ATTCCCAAAT 
TGGTCCCAAA AAGGACTAGC TATTTATTCC CACGTTGAGT TTATCTTTGT CATACTTATT 
ATTTGCGATA TAGGTATGCG CGCCTCTGTT AAAGTATCGA CTTCGGCAAA CTGTAACAAT 
ATCCTTCTAA AAACTGACAG GGGAATTTCG ATTGTTCTCT TCATCGAATC ACTGGCAAGG 
TTAGTATTAT ATCTGCCCAA TATGTGGAAG TTTTTAACAA AACCTAGTTA CGTTTACGAT 
TTTATTATAT CAATTATTAC TCTGGTTATT AGTTGCCTGG CTGTTGAAGG GGTTCTAGGA 
CATATGTATG CCTGGCTATC CATATTCCAC ATATCCAGAT TTTACAGGGT GATTATTTCT 
TTCAATTTAA CAAAAAAACT ATGGAAACAA ATATTGAGTA ATGGTGTTAT GATTTGGAAC 
TTATCGTCTT TTTACTTTTT TTTCACCTTT TTGGTTGCTA TAATCATGGC TGTGTATTTC 
GAAGGCGTGA TTCCTCCAGA AGAAATGGCA GACCAGCCAT TTGGAATGTA TTCACTACCG 
AATTCATTTC TTTCTTTGTT TATAATAGGT TCAACTGAAA ATTGGACGGA TATCCTATAT 
GCCCTCCAAA AACACTCACC AAACATCTCC TCAACTTTTT TTTGCTCAGT ATTTTTTATT 
ATATGGTTTC TACTATCCAA CTCAGTGATC TTGAACATTT TCATTGCCCT AATATCAGAA 
AGCATGGAAG TGAAAGAAGA AGAAAAACGA CCACAGCAAA TTAAGCATTA TCTTAAGTTC 
GTCTATCCTC AGAAAATACA AGAATATACA CACGCTAGTT TGGTTGCGAG AATTCGTAAA 
AAATTCTTTG GAGGTCATAG GAACGAAGAT ACAAGAGATT TTAAGCAATT TCTTATGAGA 
GGAACCGCGA TAATGAACAT AGCGCAGAAT ATGGGGGAGT TGGCCGATGA ATTCAAAGAG 
CCACCTTCAG AAAACCTATT TAAAAAGGGC TTATCAAAGC TCACAATTGG GGTTCCGTCA 
CTAAAAAGGC TGAGAATGTT TGCTAATAAT CCATTTTATA AAAATAGTGA CGTTGTGTTT 
ACAGAAACGA ACGATATAAA TGGGAGGACG TATATCTTGG AGTTGAATGA GTACGAGGAT 
GAGAAGCTAG ATTATTTAAA AAAGTACCCT TTATTCAATT ACTCATATTA TTTCTTTTCT 
CCTCAGCATA GATTTCGAAG GTTCTGTCAA CGCTTGGTAC CACCAAGCAC TGGAAAAAGG 
ACTGATGGAT CACGATTTTT CGAGGATAGC ACTGATCTAT ACAATAAAAG GAGCTATTTT 
CATCATATTG AAAGAGATGT ATTTGTATTC ATTTTCGCAC TTGCCACCAT TTTACTAATT 
GTTTGCTCAT GTTATGTTAC GCCTCTATAT CGTATGCATC ACAAGATGGG AACTTGGAAT 
TGGTCCTCGG CGTTAGATTG CGCCTTCATT GGTGCCTTCT CAATTGAATT TATCGTGAAA 
ACAGTAGCTG ACGGATTTAT ATATTCTCCA AATGCTTACC TGAGGAATCC ATGGAACTTT 
ATTGATTTTT GTGTCCTAAT CTCAATGTGG ATTAATTTAA TTGCATACCT AAAAAACAAT 
GGAAATTTGT CTAGGATTTT CAAGGGATTG ACAGCCCTGA GGGCCCTCAG ATGCCTCACG 
ATCAGTAACA CAGCTCGTCA AACATTTAAC CTAGTTATGT TTGATGGTTT AAATAAAATT 
TTTGAAGCTG GGTTGATTTC ACTCAGTTTG CTATTTCCAT TTACAGTTTG GGGCTTAAGC 
ATTTTTAAAG GCCGTTTAGG TACTTGCAAT GACGGAAGTT TGGGCCGTGC AGATTGTTAC 
AATGAATATT CAAATTCCGT TTTTCAATGG GATATCATGT CTCCAAGGGT TTACCAGCAA 
CCATATCTTC ATTTGGACTC TTTCGCAAGC GCTTTTAGTT CATTATACCA AATCATTTCT 
TTGGAAGGAT GGGTTGATTT GTTGGAAAAT ATGATGAATA GTTCAGGAAT AGGTACACCC 
GCTACGGTAA TGGGTTCAGC AGGGAATGCT TTATTCCTCG TTCTGTTTAA TTTTTTAAGT 
ATGGTTTTCA TCCTGAACTT GTTTGTTTCA TTCATTGTTA ACAACCAAGC AAGGACAACA 
GGAAGCGCTT ACTTTACCAT TGAGGAAAAG GCGTGGCTGG AATCCCAGAA ACTTTTATCT 
CAGGCCAAGC CAAAAGCTAT CCCAAATTTA ATTGAGTTAT CAAGAGTTAG GCAATTTTTC 
TATCAACTTG CAGTGGAGAA AAAAAATTTC TACTACGCAT CGTTTCTTCA GGTAGTACTT 
TATTTGCACA TAATCATGCT CCTGAGTCGA AGCTACAATC CAGGAAACTT GATAGGTTAT 
CAAGGTGTTT ATTTTATGTT TTCCACTAGT GTTTTTTTAA TTCAAGAGGC ACTTCACATG 
TGCGGTGAAG GACCAAGATT ATATTTTAGG CAAAAATGGA ACAGCATACG ACTCAGTATC 
ATAATTATAG CCTTTATTAT GAACGCTGTA GCATTCCACG TTCCAGCCTC TCACTATTGG 
TTCCACAATA TAAAGGGGTT TTTCCTGTTA GTGATATTTT TGTTTATTAT TCCTCAAAAT 
GACACACTAA CTGAACTATT AGAAACCGCA ATGGCAAGCT TACCGCCTAT TCTATCATTG 
ACCTACACTT GGGGGGTTTT ATTTTTAGTA TATGCTATTG CTTTGAATCA AATCTTCGGC 
CTAACAAGGT TAGGGAGTAA TACGACCGAT AACATAAATT TTAGAACTGT AATCAAATCC 
ATGATTGTTC TGTTTAGATG TAGTTTTGGT GAGGGCTGGA ATTATATCAT GGCCGACCTA 
ACTGTGTCAG AACCTTATTG CTCCTCTGAT GATAATTCAA CCTATACGGA CTGTGGATCA 
GAGACATATG CCTATTTGTT ATTAATGTCG TGGAATATTA TTTCCATGTA TATTTTTGTG 
AATATGTTTG TTTCGTTGAT TATTGGTAAT TTCAGTTATG TTTACCGTAG CGGTGGATCT 
CGCTCTGGCA TCAACAGATC GGAGATAAAA AAATACATTG AAGCTTGGTC CAAATTTGAT 
ACTGATGGAA CTGGCGAGCT TGAGCTGTCC TACCTCCCAA GAATAATGCA TTCATTTGAC 
GGTCCTCTTT CATTTAAAAT TTGGGAAGGT AGATTGACAA TAAAAAGTCT AGTCGAGAAC 
TACATGGAGG TTAACCCAGA TGATCCATAT GACGTCAAAA TAGACCTGAT CGGACTGAAC 
AAAGAGCTGA ATACGATTGA TAAAGCAAAG ATCATACAGA GGAAGTTACA GTACAGAAGA 
TTTGTACAAA GCATTCACTA TACGAATGCT TATAATGGAT GTATCAGATT CTCGGATTTG 
TTATTACAAA TACCTCTCTA TACAGCTTAT TCTGCAAGGG AATGTCTAGG TATTGATCAA 
TATGTCCATC ATCTATATAT CCTGGGTAAA GTGGACAAGT ACTTAGAAAA TCAAAGAAAC 
TTCGATGTAT TGGAGATGGT GGTAACAAGA TGGAAATTTC ATTGCAGGAT GAAACGTACC 
ATTGAACCCG AATGGGATGT TAAAGATCCC ACAGTATCGT CTCACATTTC GAATATAAAC 
GTAAATCTGG AACCTGCTCC AGGAATTTTA GAAAGAGAAC CTATTGCGAC ACCTAGAATG 
GACTACGGTG TTAACAATTT TATGTGGTCT CCGAGAATGA ATCAAGACTC TACGATGGAG 
CCCCCGGAAG AACCAATAGA TAATAATGAC GATAGCGCAA ATGATCTAAT TGATAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011733.3
CDS	1..6120
Translation	MQGRKRTLTEPFEPNTNPFGDNAAVMTENVEDNSETDGNRLESKPQALVPPALNIVPPESSIHSTEEKKGDEYNGNDKDSSLISNIFRTRVGRSSHENLSRPKLSLKTASFGAAESSRRNVSPSTKSAKSSSQYIDLNDERLRRRSFSSYSRSSSRRVSNSPSSTDRPPRSAKVLSLIAADDMDDFEDLQKGFKSAIDEEGLTWLPQLKSEKSRPVSDVGEDRGEGEQESIPDVHTPNVGASATPGSIHLTPEPAQNGSVSEGLEGSINNSRKKPSPKFFHHLSPQKEDKDQTEVIEYAEDILDFETLQRKLESRPFVLYGHSLGVFSPTNPLRIKIARFLLHRRYSLLYNTLLTFYAILLAIRTYNPHNVVFLYRFSNWTDYFIFILSACFTGNDIAKIIAFGFWDDSEMFKAYGREYKSILQRSGIMKLYIYLREKYGRKLIDFIIPFRIISPGEETKYQRSSLSTSLTKPYGAKENQRPFGTPRAFARSSWNRIDLVSSVSFWLGMFLSIKSYDTKTGIRIFKPLAILRILRLVNVDTGMPSILRGLKYGIPQLVNVSSMLVYFWIFFGILGVQIFQGSFRRQCVWFNPEDPTDTYQYDMQFCGGYLDPVTKRKQNYIYEDGSEGSVSKGFLCPQYSKCVSNANPYNGRISFDNIVNSMELVFVIMSANTFTDLMYYTMDSDEMAACLFFIVCIFVLTIWLLNLLIAVLVSSFEIANEEYKKKKFIYGSRKTGYVARIVTGYWKYFKLKANQTKFPNWSQKGLAIYSHVEFIFVILIICDIGMRASVKVSTSANCNNILLKTDRGISIVLFIESLARLVLYLPNMWKFLTKPSYVYDFIISIITLVISCLAVEGVLGHMYAWLSIFHISRFYRVIISFNLTKKLWKQILSNGVMIWNLSSFYFFFTFLVAIIMAVYFEGVIPPEEMADQPFGMYSLPNSFLSLFIIGSTENWTDILYALQKHSPNISSTFFCSVFFIIWFLLSNSVILNIFIALISESMEVKEEEKRPQQIKHYLKFVYPQKIQEYTHASLVARIRKKFFGGHRNEDTRDFKQFLMRGTAIMNIAQNMGELADEFKEPPSENLFKKGLSKLTIGVPSLKRLRMFANNPFYKNSDVVFTETNDINGRTYILELNEYEDEKLDYLKKYPLFNYSYYFFSPQHRFRRFCQRLVPPSTGKRTDGSRFFEDSTDLYNKRSYFHHIERDVFVFIFALATILLIVCSCYVTPLYRMHHKMGTWNWSSALDCAFIGAFSIEFIVKTVADGFIYSPNAYLRNPWNFIDFCVLISMWINLIAYLKNNGNLSRIFKGLTALRALRCLTISNTARQTFNLVMFDGLNKIFEAGLISLSLLFPFTVWGLSIFKGRLGTCNDGSLGRADCYNEYSNSVFQWDIMSPRVYQQPYLHLDSFASAFSSLYQIISLEGWVDLLENMMNSSGIGTPATVMGSAGNALFLVLFNFLSMVFILNLFVSFIVNNQARTTGSAYFTIEEKAWLESQKLLSQAKPKAIPNLIELSRVRQFFYQLAVEKKNFYYASFLQVVLYLHIIMLLSRSYNPGNLIGYQGVYFMFSTSVFLIQEALHMCGEGPRLYFRQKWNSIRLSIIIIAFIMNAVAFHVPASHYWFHNIKGFFLLVIFLFIIPQNDTLTELLETAMASLPPILSLTYTWGVLFLVYAIALNQIFGLTRLGSNTTDNINFRTVIKSMIVLFRCSFGEGWNYIMADLTVSEPYCSSDDNSTYTDCGSETYAYLLLMSWNIISMYIFVNMFVSLIIGNFSYVYRSGGSRSGINRSEIKKYIEAWSKFDTDGTGELELSYLPRIMHSFDGPLSFKIWEGRLTIKSLVENYMEVNPDDPYDVKIDLIGLNKELNTIDKAKIIQRKLQYRRFVQSIHYTNAYNGCIRFSDLLLQIPLYTAYSARECLGIDQYVHHLYILGKVDKYLENQRNFDVLEMVVTRWKFHCRMKRTIEPEWDVKDPTVSSHISNINVNLEPAPGILEREPIATPRMDYGVNNFMWSPRMNQDSTMEPPEEPIDNNDDSANDLIDR

Target ORF information:

RefSeq Version	NM_001181346.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Cch1p (CCH1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181346.3

ORF Insert Sequence:

ATGCAGGGGA GAAAAAGGAC GCTTACGGAA CCATTTGAGC CAAATACCAA TCCATTTGGG 
GACAATGCAG CAGTAATGAC GGAAAATGTT GAGGATAACA GCGAAACAGA TGGTAACCGT 
CTAGAATCAA AACCACAAGC TTTGGTCCCA CCAGCTTTAA ATATCGTGCC ACCAGAGAGC 
AGCATCCACA GTACTGAAGA AAAAAAAGGT GACGAGTACA ATGGAAATGA TAAAGATAGC 
TCCTTAATCT CCAACATATT TCGTACTCGT GTCGGAAGGA GTAGTCATGA AAACTTGAGC 
AGGCCTAAAC TCTCACTTAA AACAGCATCA TTTGGTGCCG CTGAATCTTC CCGGCGTAAT 
GTTTCACCCT CTACAAAATC TGCCAAGTCT AGTTCGCAGT ATATTGATTT AAATGATGAA 
AGGCTACGCA GGCGTAGCTT CAGTAGTTAT AGCCGATCAT CTAGTAGGCG TGTTTCTAAT 
TCACCAAGCT CAACGGATAG GCCTCCACGG TCGGCAAAGG TTTTATCGCT AATTGCCGCT 
GATGATATGG ATGATTTTGA AGATTTGCAA AAGGGATTTA AAAGTGCAAT AGACGAAGAG 
GGCCTGACAT GGCTACCCCA ATTAAAATCA GAAAAAAGCC GTCCTGTATC AGACGTTGGA 
GAAGATAGAG GAGAAGGAGA ACAAGAATCT ATACCTGACG TTCATACACC CAATGTAGGA 
GCAAGCGCTA CTCCAGGATC AATTCATCTA ACACCCGAAC CCGCGCAGAA TGGTTCGGTA 
TCTGAGGGTC TAGAAGGCTC TATTAATAAT TCAAGAAAGA AACCCAGTCC AAAGTTTTTT 
CATCATTTAT CACCGCAAAA AGAAGATAAA GACCAAACAG AAGTTATTGA GTATGCTGAA 
GACATTCTAG ATTTTGAAAC CCTTCAAAGA AAACTGGAAT CAAGGCCCTT TGTGCTTTAT 
GGACATTCTC TTGGGGTTTT CTCGCCTACG AATCCGCTAA GAATAAAAAT TGCTCGTTTT 
TTGTTGCATA GGCGGTATTC GTTACTTTAC AACACTTTGT TAACATTTTA TGCCATTCTC 
CTGGCGATAA GGACATATAA CCCTCACAAT GTGGTTTTTT TATACCGTTT CTCTAACTGG 
ACCGACTATT TCATTTTTAT TTTATCAGCT TGCTTTACAG GCAATGATAT TGCTAAAATA 
ATTGCGTTTG GATTTTGGGA CGATTCTGAA ATGTTTAAAG CCTATGGACG TGAGTATAAA 
TCAATCTTAC AGAGATCTGG AATTATGAAA CTATACATAT ATCTGAGAGA AAAGTATGGT 
AGAAAGCTAA TAGATTTCAT TATTCCATTT AGGATCATAT CGCCGGGAGA AGAGACAAAA 
TATCAACGAA GTTCGTTGAG TACTTCCCTG ACGAAACCTT ATGGGGCAAA GGAAAATCAG 
AGGCCTTTTG GCACCCCAAG AGCCTTTGCG AGATCATCGT GGAATAGAAT AGATCTGGTA 
TCTTCTGTCA GTTTTTGGCT AGGTATGTTT TTATCCATAA AAAGTTATGA TACAAAAACA 
GGCATAAGAA TATTCAAGCC CCTTGCTATA TTAAGGATTC TTCGACTTGT AAACGTGGAT 
ACTGGTATGC CCTCAATTCT AAGGGGATTG AAATATGGTA TCCCACAGTT GGTAAATGTT 
AGTTCAATGC TAGTTTACTT TTGGATTTTC TTTGGGATTT TGGGAGTACA GATTTTTCAG 
GGCTCTTTTC GAAGACAATG TGTATGGTTT AACCCTGAAG ATCCTACCGA TACTTACCAG 
TACGATATGC AATTCTGTGG TGGTTACCTA GATCCAGTAA CAAAACGAAA ACAAAATTAT 
ATCTATGAAG ACGGATCAGA AGGTTCTGTT TCAAAAGGCT TTCTTTGCCC ACAATATTCG 
AAATGTGTTT CCAATGCCAA CCCCTACAAC GGCAGAATTA GTTTTGATAA TATTGTTAAT 
TCCATGGAGC TCGTTTTTGT CATTATGAGT GCGAATACTT TTACTGACTT AATGTACTAT 
ACGATGGATT CGGATGAAAT GGCTGCCTGT TTGTTTTTTA TTGTTTGCAT CTTTGTTTTA 
ACTATCTGGC TGCTAAATCT ACTCATTGCA GTGTTGGTAT CATCTTTTGA AATAGCCAAT 
GAGGAGTACA AAAAGAAAAA GTTCATATAT GGTTCTAGAA AGACAGGTTA TGTTGCTCGC 
ATAGTAACAG GCTACTGGAA ATATTTTAAG CTTAAGGCGA ATCAGACAAA ATTCCCAAAT 
TGGTCCCAAA AAGGACTAGC TATTTATTCC CACGTTGAGT TTATCTTTGT CATACTTATT 
ATTTGCGATA TAGGTATGCG CGCCTCTGTT AAAGTATCGA CTTCGGCAAA CTGTAACAAT 
ATCCTTCTAA AAACTGACAG GGGAATTTCG ATTGTTCTCT TCATCGAATC ACTGGCAAGG 
TTAGTATTAT ATCTGCCCAA TATGTGGAAG TTTTTAACAA AACCTAGTTA CGTTTACGAT 
TTTATTATAT CAATTATTAC TCTGGTTATT AGTTGCCTGG CTGTTGAAGG GGTTCTAGGA 
CATATGTATG CCTGGCTATC CATATTCCAC ATATCCAGAT TTTACAGGGT GATTATTTCT 
TTCAATTTAA CAAAAAAACT ATGGAAACAA ATATTGAGTA ATGGTGTTAT GATTTGGAAC 
TTATCGTCTT TTTACTTTTT TTTCACCTTT TTGGTTGCTA TAATCATGGC TGTGTATTTC 
GAAGGCGTGA TTCCTCCAGA AGAAATGGCA GACCAGCCAT TTGGAATGTA TTCACTACCG 
AATTCATTTC TTTCTTTGTT TATAATAGGT TCAACTGAAA ATTGGACGGA TATCCTATAT 
GCCCTCCAAA AACACTCACC AAACATCTCC TCAACTTTTT TTTGCTCAGT ATTTTTTATT 
ATATGGTTTC TACTATCCAA CTCAGTGATC TTGAACATTT TCATTGCCCT AATATCAGAA 
AGCATGGAAG TGAAAGAAGA AGAAAAACGA CCACAGCAAA TTAAGCATTA TCTTAAGTTC 
GTCTATCCTC AGAAAATACA AGAATATACA CACGCTAGTT TGGTTGCGAG AATTCGTAAA 
AAATTCTTTG GAGGTCATAG GAACGAAGAT ACAAGAGATT TTAAGCAATT TCTTATGAGA 
GGAACCGCGA TAATGAACAT AGCGCAGAAT ATGGGGGAGT TGGCCGATGA ATTCAAAGAG 
CCACCTTCAG AAAACCTATT TAAAAAGGGC TTATCAAAGC TCACAATTGG GGTTCCGTCA 
CTAAAAAGGC TGAGAATGTT TGCTAATAAT CCATTTTATA AAAATAGTGA CGTTGTGTTT 
ACAGAAACGA ACGATATAAA TGGGAGGACG TATATCTTGG AGTTGAATGA GTACGAGGAT 
GAGAAGCTAG ATTATTTAAA AAAGTACCCT TTATTCAATT ACTCATATTA TTTCTTTTCT 
CCTCAGCATA GATTTCGAAG GTTCTGTCAA CGCTTGGTAC CACCAAGCAC TGGAAAAAGG 
ACTGATGGAT CACGATTTTT CGAGGATAGC ACTGATCTAT ACAATAAAAG GAGCTATTTT 
CATCATATTG AAAGAGATGT ATTTGTATTC ATTTTCGCAC TTGCCACCAT TTTACTAATT 
GTTTGCTCAT GTTATGTTAC GCCTCTATAT CGTATGCATC ACAAGATGGG AACTTGGAAT 
TGGTCCTCGG CGTTAGATTG CGCCTTCATT GGTGCCTTCT CAATTGAATT TATCGTGAAA 
ACAGTAGCTG ACGGATTTAT ATATTCTCCA AATGCTTACC TGAGGAATCC ATGGAACTTT 
ATTGATTTTT GTGTCCTAAT CTCAATGTGG ATTAATTTAA TTGCATACCT AAAAAACAAT 
GGAAATTTGT CTAGGATTTT CAAGGGATTG ACAGCCCTGA GGGCCCTCAG ATGCCTCACG 
ATCAGTAACA CAGCTCGTCA AACATTTAAC CTAGTTATGT TTGATGGTTT AAATAAAATT 
TTTGAAGCTG GGTTGATTTC ACTCAGTTTG CTATTTCCAT TTACAGTTTG GGGCTTAAGC 
ATTTTTAAAG GCCGTTTAGG TACTTGCAAT GACGGAAGTT TGGGCCGTGC AGATTGTTAC 
AATGAATATT CAAATTCCGT TTTTCAATGG GATATCATGT CTCCAAGGGT TTACCAGCAA 
CCATATCTTC ATTTGGACTC TTTCGCAAGC GCTTTTAGTT CATTATACCA AATCATTTCT 
TTGGAAGGAT GGGTTGATTT GTTGGAAAAT ATGATGAATA GTTCAGGAAT AGGTACACCC 
GCTACGGTAA TGGGTTCAGC AGGGAATGCT TTATTCCTCG TTCTGTTTAA TTTTTTAAGT 
ATGGTTTTCA TCCTGAACTT GTTTGTTTCA TTCATTGTTA ACAACCAAGC AAGGACAACA 
GGAAGCGCTT ACTTTACCAT TGAGGAAAAG GCGTGGCTGG AATCCCAGAA ACTTTTATCT 
CAGGCCAAGC CAAAAGCTAT CCCAAATTTA ATTGAGTTAT CAAGAGTTAG GCAATTTTTC 
TATCAACTTG CAGTGGAGAA AAAAAATTTC TACTACGCAT CGTTTCTTCA GGTAGTACTT 
TATTTGCACA TAATCATGCT CCTGAGTCGA AGCTACAATC CAGGAAACTT GATAGGTTAT 
CAAGGTGTTT ATTTTATGTT TTCCACTAGT GTTTTTTTAA TTCAAGAGGC ACTTCACATG 
TGCGGTGAAG GACCAAGATT ATATTTTAGG CAAAAATGGA ACAGCATACG ACTCAGTATC 
ATAATTATAG CCTTTATTAT GAACGCTGTA GCATTCCACG TTCCAGCCTC TCACTATTGG 
TTCCACAATA TAAAGGGGTT TTTCCTGTTA GTGATATTTT TGTTTATTAT TCCTCAAAAT 
GACACACTAA CTGAACTATT AGAAACCGCA ATGGCAAGCT TACCGCCTAT TCTATCATTG 
ACCTACACTT GGGGGGTTTT ATTTTTAGTA TATGCTATTG CTTTGAATCA AATCTTCGGC 
CTAACAAGGT TAGGGAGTAA TACGACCGAT AACATAAATT TTAGAACTGT AATCAAATCC 
ATGATTGTTC TGTTTAGATG TAGTTTTGGT GAGGGCTGGA ATTATATCAT GGCCGACCTA 
ACTGTGTCAG AACCTTATTG CTCCTCTGAT GATAATTCAA CCTATACGGA CTGTGGATCA 
GAGACATATG CCTATTTGTT ATTAATGTCG TGGAATATTA TTTCCATGTA TATTTTTGTG 
AATATGTTTG TTTCGTTGAT TATTGGTAAT TTCAGTTATG TTTACCGTAG CGGTGGATCT 
CGCTCTGGCA TCAACAGATC GGAGATAAAA AAATACATTG AAGCTTGGTC CAAATTTGAT 
ACTGATGGAA CTGGCGAGCT TGAGCTGTCC TACCTCCCAA GAATAATGCA TTCATTTGAC 
GGTCCTCTTT CATTTAAAAT TTGGGAAGGT AGATTGACAA TAAAAAGTCT AGTCGAGAAC 
TACATGGAGG TTAACCCAGA TGATCCATAT GACGTCAAAA TAGACCTGAT CGGACTGAAC 
AAAGAGCTGA ATACGATTGA TAAAGCAAAG ATCATACAGA GGAAGTTACA GTACAGAAGA 
TTTGTACAAA GCATTCACTA TACGAATGCT TATAATGGAT GTATCAGATT CTCGGATTTG 
TTATTACAAA TACCTCTCTA TACAGCTTAT TCTGCAAGGG AATGTCTAGG TATTGATCAA 
TATGTCCATC ATCTATATAT CCTGGGTAAA GTGGACAAGT ACTTAGAAAA TCAAAGAAAC 
TTCGATGTAT TGGAGATGGT GGTAACAAGA TGGAAATTTC ATTGCAGGAT GAAACGTACC 
ATTGAACCCG AATGGGATGT TAAAGATCCC ACAGTATCGT CTCACATTTC GAATATAAAC 
GTAAATCTGG AACCTGCTCC AGGAATTTTA GAAAGAGAAC CTATTGCGAC ACCTAGAATG 
GACTACGGTG TTAACAATTT TATGTGGTCT CCGAGAATGA ATCAAGACTC TACGATGGAG 
CCCCCGGAAG AACCAATAGA TAATAATGAC GATAGCGCAA ATGATCTAAT TGATAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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CCH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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