smco-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	smco-1
Entrez Gene ID	3879881
Full Name	semicolonial-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_958639.3	XP_963732.3	calcium channel subunit Cch1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ACL143C	NP_983261.1
Schizosaccharomyces pombe (fission yeast)	cch1	NP_593894.1
Saccharomyces cerevisiae (baker's yeast)	CCH1	NP_011733.3
Kluyveromyces lactis	KLLA0E16083g	XP_454673.1
Neurospora crassa	NCU02762	XP_963732.2
Magnaporthe oryzae (rice blast fungus)	MGG_05643	XP_003710534.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following smco-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smco-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12531	XM_958639.3 Latest version!	Neurospora crassa OR74A calcium channel subunit Cch1 (smco-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smco-1 ( XM_958639.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_963732.3 Neurospora crassa OR74A calcium channel subunit Cch1 (smco-1), mRNA.

Price & Availability↑

CloneID	ONf12531
Clone ID Related Accession (Same CDS sequence)	XM_958639.3
Accession Version	XM_958639.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	calcium channel subunit Cch1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_958639.2.

ATGAGCGATC ATCCAGCACC TTCGCCCCGA CGGGCCTCGG TATCCTCTCC TTCTCACCAG 
CAGTCCATTC CATTGCGCGA TCTACGACCG GTTTCGGAGG AGCAAGAGGG CGGCGGTGGG 
AGGACTAGCG GAACCGATAA TAACGTCCGC AACTCGGGAG CCGGACTTCA GATGCCCTCG 
CCTTTGGCTG CTCCCATGTC CGCCAACCTT CCCGGGCTCT CTACGTACTG GGAACATCCA 
TATGCGCCCG GCCACGACCA CCGAAGCGCC GCTGGTTCGC TCAACGACCA CATGGCGCTT 
CAGTTCGCCT TGCCTCCGAA TATTCCTGGC CATGGTCATG AACCGCTCCC TTCAGCCTCC 
TATATGCCTG CCGCAAGTGA CCCCTACAGT GTGCCGCATC CCTATTACGA GGAGCGTACC 
GATACGGACT CTGTGGAGTC GGATAGAGTC CCTCTGAAGC CCGCAGCCCA GCCCATCGGT 
CGATTGGACG CGCCAGAAGG AGAGGCTGTA CCAAGAGATA GTTTCCAGAC CGTCTCTGAT 
CTTGGCAGTG GCTCCTCGCG ACCCCGCAAC ACCATGCTTG GTTTTGATCT GGAAAACAAC 
TTTACCGCGG ACCGTTACCG GAGTTATGGA GGTAGTCTGG TTCCTAGTGA TGGACGCCGC 
TCTCGGTCGC TATCAACGAG TGGCGCTTTG TCAAGGGCAG GTTCCATTAT GAGGGCCATG 
TCTCAGCGTG TTGTCATGAT CAGTGGAGAG GGTGATTTGA TTGACCAGCA AACGCAACGA 
GACCGCTCAC GCTCGCCGAG CATGGATCGC CCGCGGACGG GCCACGCATC GGGCCCCATG 
CTGGTTGACA CCTCCTATCC GTCACAAATG GAACAAAACC CCACGGAAAA GCGACCTGAA 
TCGCATCATG TCTTTCCACA GCACCCGGCC TTCAGGCCCC GGTCACGCAA TCCTAGGAAC 
AATCCGCTCA AGGGCAAGTC GTTGGGGATA TTTTCCCCTG ACAATCCACT GCGCTTGTGG 
CTTTGCGATT TGTTGGTCAA CCCATGGGCT GAGCCACTCA TTCTCATCTT AATCGTTCTT 
CAAGCCGTCC TCCTAGCTGT TGAGGCTGCG CCAGATGTGC TGGCTGATGG AAACGGTCGG 
CCTGAGCGTT GGGGTAGCAC CAGGATAGAC TGGGCCATAT TTGTCCTGTT TGTCATCTTT 
ACTATCGAAC TCATAGCGAG AATTATTGTG TCTGGCTTCA TCATCAACGC CCCCGAATAC 
GAAGAAGCCA AGAACAAACG GCGCTTCCGT GAAAGGCTGG CCGAACAGTA TCGGGTTATA 
TTCCGACCGC AGCGTCAGAG ATCGGTTCGT CAAACCCGGC AAGAGCCCTT CGAGCCTGCT 
CCGTTCACGA GATCCTTTAC CATGGCCCAC AGTCAAATTC AAACGCTCGA GGATCAACAG 
CGACGTCACC TGGCTCGCCG CGCCTTTCTC CGCCATGGCT TTAATAGGTT AGACTTCGTA 
GCCGTTGTCT CCTTCTGGAT ATCCTTCGTA TTAGGCGTCA CTGGTCTTGA GAGCAAGCAC 
CATATGTACG TCTTCCGCAT GCTAAGCTGC TTGCGCATCA TTCGATTACT CGCCCTTACA 
AAGGGAAATC TGATCATCTT ACGAAGTCTG AAGAAGGCCG CGCCGTTGCT CGTCCGAGTT 
TCCTTCTTGA TGGGTTTCTT CTGGCTTCTC TTTGCCATCA TTGGCGTGCA GAGCTTTAAG 
TCAAGTCTGA GTCGTCAATG TCTTTGGATA GATCCCGATG ATCCCACCAA CTTCCAAGCA 
GCTTATACGC CGGATATGAC CTTTTGTGGA GGGCATATTG ACAACGTGAC GGGTGAAACC 
ATGCCCTGGG TTTATAGTCA CGAAAAGGAA TCCCTAATTG ATTTGAAGAA CGGAACTTCG 
CGTGCCAAGG GCTTCATCTG CCCGAGAGGT TCAATATGCA TCCGGCAAGA AAACCCCTTC 
AATGGAACGG TCAATTTCGA CGATATCGGC CATTCTTTGG AGCTCGTGTT TGTCATCATG 
AGCGCCAACA CCTGGTCGGA CTTGATGTAT CACACTATCC GCTCAGATTT CCTCCCGTCG 
GCCCTCTTCT ATGGTGCTGG CATTCTTATA ATGTTGCTCT GGATGACCAA CTTGTTGATT 
GCCGTCATCA CGTCGTCGTT TCAGGTCATC CGAGAAGAGT CCAAGGCTAG CGCTTTTACT 
GCGGATGAAC CTCCTGGCAT GACTTCATCT CGAGTTGATG ATACCATGCG TAGGCCGACA 
TTGCTGCAAA GAATATATAA CAGGACAAAA CCTTTGTGGA TCTTGATCAT CGCTTTTGGA 
CTTTTGGCAC AGTCTCTCCG CAGCGCCAGG ATGAAGTCAG ACCGAGAGCG TTTCGTCAAT 
ATAGCAGAGC CTGTGGTCAC TCTTCTGCTC GATGTCGAAA TCATCATCCG CTTGGCTGCA 
GACATCCGAG GTTTTCACCG TAGGAAACGG AACCTTGTCG ACTTGGGGCT TGCTGTAGCC 
ACCTCAATCA TCCTCGTCCC TCCTATACGG AATTCTGGCC AAGTTTACAA TTGGCTTACC 
GCGTTCCAAA TCCTGCGAGT TTACCGTTTA GTCATGGTTA TTCCCATGAC AAGGAAATTA 
ATCCGGCTTG TGCTCGGAAA CGTCACTGGT ATCGGTAATC TAATGTCGTT TGTCTTCCTC 
ATCACTTTTC TGATGGCCAT CTTCGCAGCG CAATTATTCC GTGGCGAACT ACCGAGAGAA 
GATGATGGTG GAGAGGAAAT TAGAATCCCG TTCAACACCA TTTATAATTC CTTCTTAGGG 
ATGTATCAAG TTATGTCGAC TGAGAACTGG ACCGACATCC TCTACAGCGT CACTGCCAGC 
ACTCACCACC TGCACACTGC TTGGATTGGC GCCATTTTTC TCGTCGGCTG GTTCATTCTC 
GCTTATTTTA TCTTGATCAA CATGTTCATT GCCGTCATCC AAGAAAATTT TGATGTTGGA 
GAGGATGTCA AGCGTCTTGA ACAGGTTAAG GCATTCCTCC AGCGCAAGGA ACTAGGACAT 
TCTTCTTCGA GCAACCTGGC ATTATCCAAA GTTTTCAATT TTGGCCGGTC AAGAAAACAG 
AGGGATCCGC TCGACTATGG CCCTGCTATG ATGGAGATGC TTCTGAAAGA GGCCGTGGTA 
CACGACTTCC TTGACGACGA TATGGACCCT CTCCAGCAAA GCTCTCCCGA TGGTCAGCCA 
ACTTCCCATC TGGGTAGTAG TGCAGCCATG CAACATGACG GTGTCACTCC CGGGGTACTC 
TCCTCTTTTT GGGGAAAACT CGTGTCAGTG TTTACTAGCC GTGAACCCAA CCCTTTCTAT 
TCGAACGTCA ATTTGCACGG GCTGGGTGAG AATCTCGATC CACGAACTAT GGCCGCCCAG 
GCGATGAATG CCACGAGACG CCGCCAGAGG CAACAGAGAG AGTATTTGAT GCGCCACCCC 
AACTACAACA ATTCCCTGTT CATTTTCAAA CCCAGAAATC CCATACGACG TCTTTGTCAA 
AGTCTGGTCG GCCCTGGTCG GGGAACTGAG CGCTTCGAGG GGGCGCAGCC CAATAAATAT 
GCATGGTACA CCTTCTCGAC TCTGATTTAT GCAGCCATCG TGATCATGGT GGTGCTTGCT 
TGCATTACTA CACCCTTGTA CCAAAAGGAA TACTTCGAAA AGTATGGCGA GAATGCGTTT 
GACTGGTTCC GCTGGGTAGA CACGGGGTTT GCCATTGTCT TCACCCTTGA AGCTCTCATC 
AAGGTTATAG CAGACGGCTT TCTTTGGACA CCAAATGCAT ACTTCCGCAG CACCTGGGGT 
GTGATTGATG CCATTGTGCT CATAACTCTT TGGATCAACG TCATCACTCT ATTTACCCGA 
AACGGAGCCG TGTCTAGAGC TGTGGGTGCC TTCAAAGCAT TAAGGGCCCT GCGCCTGCTC 
AACATCAGCG AAAGTGCGAT GGACACTTTC CACTCGCTCC TCATCGTGGG TGGGTGGAAG 
ATCTTGAGCG CTGCTTTCGT TTCTATATCC CTCCTTATTC CCTTTGCTAT TTATGGGGTC 
AACCTTTTCC ACGGTAAACT GCTTGCATGC AACGACAGCT CGGAGGGAAT AGTCAGCCTG 
GATGACTGCT TTGGTGAATG GAACAGCACC CCTTACAACA ACAATTGGCC CGTTCTTGCT 
CCGAGATCAG TAGCGAACCC ATACTTCAGC TTTGACGATT TTGGCTCGTC TCTCTTCATT 
CTTTTCCAGA TTGTGAGCCA AGAGGGCTGG GTTGACGTGT CTTTTGCTCT TCAAGCCATT 
ACCGGTAGGG GTTTGCAGCC GCAGTCGGGG GCTCCCTACA GCGGCCAAGG AAATGCGTTG 
TTCTTGGTCG TCTTCAACCT ATTGGCGACA GTCTTTGTGT TGACGCTCTT TATCTCTGTG 
TTCATGAGGA ACTATACTGA ACAAACAGGT GTTGCTTTCC TGACTGCCGA GCAAAGATCC 
TGGCTCGAGC TGCGGAAGCT TCTCCGCCAA ATCTCGCCGT CCAAAAGCTC ATATGACGAG 
AGTAAGAACA AGTGGAAGAT ATGGTGCCAT AAACGTGCCA TTGAGAAGCG AGGGAAATGG 
TATATGGCCA TCACCAGCGT TCTGGTCTTG CATCTTATAC TCCTTCTCTC CGAGTTCTAC 
AGCGAGCCAG ATTGGTGGAC AAACACCAGG GACGCTTTGT TCTTACTCTT CACACTCATC 
TACATCGCCA ACATCGTGAT TCGGATTGTG GGGCTTGGCT GGAGCCGCTT TCGCAAGAGT 
TCTTGGGACA TGTTCTCCCT CATCACTGTT ACTGGTGCAT TCGCCACGAC GATCCTTTTC 
ATGACGAACA CGAAGCTGGA CACGTACATT CAATTACACA AGTTCTTTCT GGTGGCGATC 
GTCCTAATGC TCATTCCCCG CAATGATGCG CTTGATCAAC TGTTCAAGAC AGCTGCCGCC 
AGTCTGACAA CTATCGGAAG CCTGTTGGCC ACATGGCTCG TTTTCTTTCT GGTGTTTGCG 
ATTGCACTTA CACAGACATT TAGTCTCACC CGTTTCGGAA ATAGCACTGA TTACTATGTC 
AATCTCCGTA CGGTGCCGAA TGCCCTGATC CTACTCTTCC GGTTCAGTTG CGGAGAGGGC 
TGGAATCAGG TTATGGAAGA CTTTGCTAAG ATCAAACCGC CGCTTTGTGT GGAGAGCACC 
AATTTCTCTG ACAGCGATTG CGGCAGCACC GCATGGGCGC GTGCTCTTTT CATCGCGTGG 
AACATCATCA GCATGTACAT TTTTGTTAAC TTGTTTGTTT CGCTGATTTA CGAAAGTTTT 
TCGTACGTAT ACCAGCGGAC AAGCGGACTT GCCATGGTGG ATCGGGACGA GCTCCGGCGC 
TTCAAGGAAG CATGGCGCAG TGTTGACCCT TCAGGGACTG GATTTATCAG CAAGGAGGCT 
TTCCCGCGCC TCTTGGGTGA ACTGTCGGGC GTGTTCGAGA TGCGCATTTA TGAAAGCCAT 
GACTCGGTGC GCCAGATCCT CGAAGATGTT CGTGGTGAAG ACACGTCAAG CATCAGACAT 
GCCTCGATCA TTAGTACCAC GCAGTTTCAA ACTAGCTTGG ATCTCAAAAA GTTAAACGAA 
CGGATATCAC AAATTGATGT CGCTAAGGTG CGCGAACGCA GACGGCGGTT CAACATCTTC 
TTCGAGGAGG TGATGGTGTC TGCGGATCCA GAAAGAGGCA TTTCCTTTAC CACTGTTCTT 
ATGATTCTGG CACATTATAA GATTATTAAC GACAGCAAGA GCTTACGTCT CGAGGAATTC 
CTTCGTCGCC GCTCACGTCT TCAACGCGTT GAGGAGGAAA TACACCGTCG CGTCGTCCTC 
GGGTTCTTCG ACATGATTTA CTACTCTCGC AAGTTTCAGC GCCACATGGA GCTCAAGCGG 
TCTGCTCGGC TCACGGCGAT TCCACAGCTG GGCGTACCTG AAATTTACGT GGATAACGAT 
CATGAAAATC AAACAGACAA TGAACAGCAA AACAAGGACA GCAACAGCAA AAACAGTGCG 
AGAGCTAGCG GAAGCGGCAG CAGTACTGCT GCTGGTACAA ATTTTTTCGA ATCCACACTC 
ACGAATCCGT TCGGCTCTGG TTCCAGTACA GTCAATCCCG GCGGTGGCGC ATCCTTTCTA 
TCAGCAGACG ATGCTCGCAC AGCTCATCAC CGCAGCTGGT CTGGGGCGAG CGCCGACCTT 
TCGTCGTTTG ATACTTCATA CGGCCATCCA CTCGCCGGCC CTCGCATGAA CAAGTCGTCC 
AACTCTGTGT CGCATCGCAA CACGCATGCC TACAGCTTCG AATTGCACGA TTCCTCAGGA 
ATTGGAGGAG GAAACGCCCC TTCGCCCGGT GGCTGGGGCG GCGGGTCAGG GACGATGGAT 
AGCATGGGCT TGGGTGGCGG GAGTGCTCTG GATGATAATA ATGGCGGAAG AAGCCCCGGC 
GGCGAGGGTA CCAAGCGCGG AAGCTCCGTC AGTCCCTCGA AACTCAGCTC GATGCTGGAT 
GATTCCGTGT GGGTGGCCAG TATCAGGAGG AGCGCGACGA TGAGACGGAG CCAGAATGAT 
ACGGATTGGG GAGGAGGGTC AAGAGCTTCC GGGGGAGGAT ACTCGGGGGC TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_963732.3
CDS	577..7233
Translation	MSDHPAPSPRRASVSSPSHQQSIPLRDLRPVSEEQEGGGGRTSGTDNNVRNSGAGLQMPSPLAAPMSANLPGLSTYWEHPYAPGHDHRSAAGSLNDHMALQFALPPNIPGHGHEPLPSASYMPAASDPYSVPHPYYEERTDTDSVESDRVPLKPAAQPIGRLDAPEGEAVPRDSFQTVSDLGSGSSRPRNTMLGFDLENNFTADRYRSYGGSLVPSDGRRSRSLSTSGALSRAGSIMRAMSQRVVMISGEGDLIDQQTQRDRSRSPSMDRPRTGHASGPMLVDTSYPSQMEQNPTEKRPESHHVFPQHPAFRPRSRNPRNNPLKGKSLGIFSPDNPLRLWLCDLLVNPWAEPLILILIVLQAVLLAVEAAPDVLADGNGRPERWGSTRIDWAIFVLFVIFTIELIARIIVSGFIINAPEYEEAKNKRRFRERLAEQYRVIFRPQRQRSVRQTRQEPFEPAPFTRSFTMAHSQIQTLEDQQRRHLARRAFLRHGFNRLDFVAVVSFWISFVLGVTGLESKHHMYVFRMLSCLRIIRLLALTKGNLIILRSLKKAAPLLVRVSFLMGFFWLLFAIIGVQSFKSSLSRQCLWIDPDDPTNFQAAYTPDMTFCGGHIDNVTGETMPWVYSHEKESLIDLKNGTSRAKGFICPRGSICIRQENPFNGTVNFDDIGHSLELVFVIMSANTWSDLMYHTIRSDFLPSALFYGAGILIMLLWMTNLLIAVITSSFQVIREESKASAFTADEPPGMTSSRVDDTMRRPTLLQRIYNRTKPLWILIIAFGLLAQSLRSARMKSDRERFVNIAEPVVTLLLDVEIIIRLAADIRGFHRRKRNLVDLGLAVATSIILVPPIRNSGQVYNWLTAFQILRVYRLVMVIPMTRKLIRLVLGNVTGIGNLMSFVFLITFLMAIFAAQLFRGELPREDDGGEEIRIPFNTIYNSFLGMYQVMSTENWTDILYSVTASTHHLHTAWIGAIFLVGWFILAYFILINMFIAVIQENFDVGEDVKRLEQVKAFLQRKELGHSSSSNLALSKVFNFGRSRKQRDPLDYGPAMMEMLLKEAVVHDFLDDDMDPLQQSSPDGQPTSHLGSSAAMQHDGVTPGVLSSFWGKLVSVFTSREPNPFYSNVNLHGLGENLDPRTMAAQAMNATRRRQRQQREYLMRHPNYNNSLFIFKPRNPIRRLCQSLVGPGRGTERFEGAQPNKYAWYTFSTLIYAAIVIMVVLACITTPLYQKEYFEKYGENAFDWFRWVDTGFAIVFTLEALIKVIADGFLWTPNAYFRSTWGVIDAIVLITLWINVITLFTRNGAVSRAVGAFKALRALRLLNISESAMDTFHSLLIVGGWKILSAAFVSISLLIPFAIYGVNLFHGKLLACNDSSEGIVSLDDCFGEWNSTPYNNNWPVLAPRSVANPYFSFDDFGSSLFILFQIVSQEGWVDVSFALQAITGRGLQPQSGAPYSGQGNALFLVVFNLLATVFVLTLFISVFMRNYTEQTGVAFLTAEQRSWLELRKLLRQISPSKSSYDESKNKWKIWCHKRAIEKRGKWYMAITSVLVLHLILLLSEFYSEPDWWTNTRDALFLLFTLIYIANIVIRIVGLGWSRFRKSSWDMFSLITVTGAFATTILFMTNTKLDTYIQLHKFFLVAIVLMLIPRNDALDQLFKTAAASLTTIGSLLATWLVFFLVFAIALTQTFSLTRFGNSTDYYVNLRTVPNALILLFRFSCGEGWNQVMEDFAKIKPPLCVESTNFSDSDCGSTAWARALFIAWNIISMYIFVNLFVSLIYESFSYVYQRTSGLAMVDRDELRRFKEAWRSVDPSGTGFISKEAFPRLLGELSGVFEMRIYESHDSVRQILEDVRGEDTSSIRHASIISTTQFQTSLDLKKLNERISQIDVAKVRERRRRFNIFFEEVMVSADPERGISFTTVLMILAHYKIINDSKSLRLEEFLRRRSRLQRVEEEIHRRVVLGFFDMIYYSRKFQRHMELKRSARLTAIPQLGVPEIYVDNDHENQTDNEQQNKDSNSKNSARASGSGSSTAAGTNFFESTLTNPFGSGSSTVNPGGGASFLSADDARTAHHRSWSGASADLSSFDTSYGHPLAGPRMNKSSNSVSHRNTHAYSFELHDSSGIGGGNAPSPGGWGGGSGTMDSMGLGGGSALDDNNGGRSPGGEGTKRGSSVSPSKLSSMLDDSVWVASIRRSATMRRSQNDTDWGGGSRASGGGYSGAY

Target ORF information:

RefSeq Version	XM_958639.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A calcium channel subunit Cch1 (smco-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_958639.3

ORF Insert Sequence:

ATGAGCGATC ATCCAGCACC TTCGCCCCGA CGGGCCTCGG TATCCTCTCC TTCTCACCAG 
CAGTCCATTC CATTGCGCGA TCTACGACCG GTTTCGGAGG AGCAAGAGGG CGGCGGTGGG 
AGGACTAGCG GAACCGATAA TAACGTCCGC AACTCGGGAG CCGGACTTCA GATGCCCTCG 
CCTTTGGCTG CTCCCATGTC CGCCAACCTT CCCGGGCTCT CTACGTACTG GGAACATCCA 
TATGCGCCCG GCCACGACCA CCGAAGCGCC GCTGGTTCGC TCAACGACCA CATGGCGCTT 
CAGTTCGCCT TGCCTCCGAA TATTCCTGGC CATGGTCATG AACCGCTCCC TTCAGCCTCC 
TATATGCCTG CCGCAAGTGA CCCCTACAGT GTGCCGCATC CCTATTACGA GGAGCGTACC 
GATACGGACT CTGTGGAGTC GGATAGAGTC CCTCTGAAGC CCGCAGCCCA GCCCATCGGT 
CGATTGGACG CGCCAGAAGG AGAGGCTGTA CCAAGAGATA GTTTCCAGAC CGTCTCTGAT 
CTTGGCAGTG GCTCCTCGCG ACCCCGCAAC ACCATGCTTG GTTTTGATCT GGAAAACAAC 
TTTACCGCGG ACCGTTACCG GAGTTATGGA GGTAGTCTGG TTCCTAGTGA TGGACGCCGC 
TCTCGGTCGC TATCAACGAG TGGCGCTTTG TCAAGGGCAG GTTCCATTAT GAGGGCCATG 
TCTCAGCGTG TTGTCATGAT CAGTGGAGAG GGTGATTTGA TTGACCAGCA AACGCAACGA 
GACCGCTCAC GCTCGCCGAG CATGGATCGC CCGCGGACGG GCCACGCATC GGGCCCCATG 
CTGGTTGACA CCTCCTATCC GTCACAAATG GAACAAAACC CCACGGAAAA GCGACCTGAA 
TCGCATCATG TCTTTCCACA GCACCCGGCC TTCAGGCCCC GGTCACGCAA TCCTAGGAAC 
AATCCGCTCA AGGGCAAGTC GTTGGGGATA TTTTCCCCTG ACAATCCACT GCGCTTGTGG 
CTTTGCGATT TGTTGGTCAA CCCATGGGCT GAGCCACTCA TTCTCATCTT AATCGTTCTT 
CAAGCCGTCC TCCTAGCTGT TGAGGCTGCG CCAGATGTGC TGGCTGATGG AAACGGTCGG 
CCTGAGCGTT GGGGTAGCAC CAGGATAGAC TGGGCCATAT TTGTCCTGTT TGTCATCTTT 
ACTATCGAAC TCATAGCGAG AATTATTGTG TCTGGCTTCA TCATCAACGC CCCCGAATAC 
GAAGAAGCCA AGAACAAACG GCGCTTCCGT GAAAGGCTGG CCGAACAGTA TCGGGTTATA 
TTCCGACCGC AGCGTCAGAG ATCGGTTCGT CAAACCCGGC AAGAGCCCTT CGAGCCTGCT 
CCGTTCACGA GATCCTTTAC CATGGCCCAC AGTCAAATTC AAACGCTCGA GGATCAACAG 
CGACGTCACC TGGCTCGCCG CGCCTTTCTC CGCCATGGCT TTAATAGGTT AGACTTCGTA 
GCCGTTGTCT CCTTCTGGAT ATCCTTCGTA TTAGGCGTCA CTGGTCTTGA GAGCAAGCAC 
CATATGTACG TCTTCCGCAT GCTAAGCTGC TTGCGCATCA TTCGATTACT CGCCCTTACA 
AAGGGAAATC TGATCATCTT ACGAAGTCTG AAGAAGGCCG CGCCGTTGCT CGTCCGAGTT 
TCCTTCTTGA TGGGTTTCTT CTGGCTTCTC TTTGCCATCA TTGGCGTGCA GAGCTTTAAG 
TCAAGTCTGA GTCGTCAATG TCTTTGGATA GATCCCGATG ATCCCACCAA CTTCCAAGCA 
GCTTATACGC CGGATATGAC CTTTTGTGGA GGGCATATTG ACAACGTGAC GGGTGAAACC 
ATGCCCTGGG TTTATAGTCA CGAAAAGGAA TCCCTAATTG ATTTGAAGAA CGGAACTTCG 
CGTGCCAAGG GCTTCATCTG CCCGAGAGGT TCAATATGCA TCCGGCAAGA AAACCCCTTC 
AATGGAACGG TCAATTTCGA CGATATCGGC CATTCTTTGG AGCTCGTGTT TGTCATCATG 
AGCGCCAACA CCTGGTCGGA CTTGATGTAT CACACTATCC GCTCAGATTT CCTCCCGTCG 
GCCCTCTTCT ATGGTGCTGG CATTCTTATA ATGTTGCTCT GGATGACCAA CTTGTTGATT 
GCCGTCATCA CGTCGTCGTT TCAGGTCATC CGAGAAGAGT CCAAGGCTAG CGCTTTTACT 
GCGGATGAAC CTCCTGGCAT GACTTCATCT CGAGTTGATG ATACCATGCG TAGGCCGACA 
TTGCTGCAAA GAATATATAA CAGGACAAAA CCTTTGTGGA TCTTGATCAT CGCTTTTGGA 
CTTTTGGCAC AGTCTCTCCG CAGCGCCAGG ATGAAGTCAG ACCGAGAGCG TTTCGTCAAT 
ATAGCAGAGC CTGTGGTCAC TCTTCTGCTC GATGTCGAAA TCATCATCCG CTTGGCTGCA 
GACATCCGAG GTTTTCACCG TAGGAAACGG AACCTTGTCG ACTTGGGGCT TGCTGTAGCC 
ACCTCAATCA TCCTCGTCCC TCCTATACGG AATTCTGGCC AAGTTTACAA TTGGCTTACC 
GCGTTCCAAA TCCTGCGAGT TTACCGTTTA GTCATGGTTA TTCCCATGAC AAGGAAATTA 
ATCCGGCTTG TGCTCGGAAA CGTCACTGGT ATCGGTAATC TAATGTCGTT TGTCTTCCTC 
ATCACTTTTC TGATGGCCAT CTTCGCAGCG CAATTATTCC GTGGCGAACT ACCGAGAGAA 
GATGATGGTG GAGAGGAAAT TAGAATCCCG TTCAACACCA TTTATAATTC CTTCTTAGGG 
ATGTATCAAG TTATGTCGAC TGAGAACTGG ACCGACATCC TCTACAGCGT CACTGCCAGC 
ACTCACCACC TGCACACTGC TTGGATTGGC GCCATTTTTC TCGTCGGCTG GTTCATTCTC 
GCTTATTTTA TCTTGATCAA CATGTTCATT GCCGTCATCC AAGAAAATTT TGATGTTGGA 
GAGGATGTCA AGCGTCTTGA ACAGGTTAAG GCATTCCTCC AGCGCAAGGA ACTAGGACAT 
TCTTCTTCGA GCAACCTGGC ATTATCCAAA GTTTTCAATT TTGGCCGGTC AAGAAAACAG 
AGGGATCCGC TCGACTATGG CCCTGCTATG ATGGAGATGC TTCTGAAAGA GGCCGTGGTA 
CACGACTTCC TTGACGACGA TATGGACCCT CTCCAGCAAA GCTCTCCCGA TGGTCAGCCA 
ACTTCCCATC TGGGTAGTAG TGCAGCCATG CAACATGACG GTGTCACTCC CGGGGTACTC 
TCCTCTTTTT GGGGAAAACT CGTGTCAGTG TTTACTAGCC GTGAACCCAA CCCTTTCTAT 
TCGAACGTCA ATTTGCACGG GCTGGGTGAG AATCTCGATC CACGAACTAT GGCCGCCCAG 
GCGATGAATG CCACGAGACG CCGCCAGAGG CAACAGAGAG AGTATTTGAT GCGCCACCCC 
AACTACAACA ATTCCCTGTT CATTTTCAAA CCCAGAAATC CCATACGACG TCTTTGTCAA 
AGTCTGGTCG GCCCTGGTCG GGGAACTGAG CGCTTCGAGG GGGCGCAGCC CAATAAATAT 
GCATGGTACA CCTTCTCGAC TCTGATTTAT GCAGCCATCG TGATCATGGT GGTGCTTGCT 
TGCATTACTA CACCCTTGTA CCAAAAGGAA TACTTCGAAA AGTATGGCGA GAATGCGTTT 
GACTGGTTCC GCTGGGTAGA CACGGGGTTT GCCATTGTCT TCACCCTTGA AGCTCTCATC 
AAGGTTATAG CAGACGGCTT TCTTTGGACA CCAAATGCAT ACTTCCGCAG CACCTGGGGT 
GTGATTGATG CCATTGTGCT CATAACTCTT TGGATCAACG TCATCACTCT ATTTACCCGA 
AACGGAGCCG TGTCTAGAGC TGTGGGTGCC TTCAAAGCAT TAAGGGCCCT GCGCCTGCTC 
AACATCAGCG AAAGTGCGAT GGACACTTTC CACTCGCTCC TCATCGTGGG TGGGTGGAAG 
ATCTTGAGCG CTGCTTTCGT TTCTATATCC CTCCTTATTC CCTTTGCTAT TTATGGGGTC 
AACCTTTTCC ACGGTAAACT GCTTGCATGC AACGACAGCT CGGAGGGAAT AGTCAGCCTG 
GATGACTGCT TTGGTGAATG GAACAGCACC CCTTACAACA ACAATTGGCC CGTTCTTGCT 
CCGAGATCAG TAGCGAACCC ATACTTCAGC TTTGACGATT TTGGCTCGTC TCTCTTCATT 
CTTTTCCAGA TTGTGAGCCA AGAGGGCTGG GTTGACGTGT CTTTTGCTCT TCAAGCCATT 
ACCGGTAGGG GTTTGCAGCC GCAGTCGGGG GCTCCCTACA GCGGCCAAGG AAATGCGTTG 
TTCTTGGTCG TCTTCAACCT ATTGGCGACA GTCTTTGTGT TGACGCTCTT TATCTCTGTG 
TTCATGAGGA ACTATACTGA ACAAACAGGT GTTGCTTTCC TGACTGCCGA GCAAAGATCC 
TGGCTCGAGC TGCGGAAGCT TCTCCGCCAA ATCTCGCCGT CCAAAAGCTC ATATGACGAG 
AGTAAGAACA AGTGGAAGAT ATGGTGCCAT AAACGTGCCA TTGAGAAGCG AGGGAAATGG 
TATATGGCCA TCACCAGCGT TCTGGTCTTG CATCTTATAC TCCTTCTCTC CGAGTTCTAC 
AGCGAGCCAG ATTGGTGGAC AAACACCAGG GACGCTTTGT TCTTACTCTT CACACTCATC 
TACATCGCCA ACATCGTGAT TCGGATTGTG GGGCTTGGCT GGAGCCGCTT TCGCAAGAGT 
TCTTGGGACA TGTTCTCCCT CATCACTGTT ACTGGTGCAT TCGCCACGAC GATCCTTTTC 
ATGACGAACA CGAAGCTGGA CACGTACATT CAATTACACA AGTTCTTTCT GGTGGCGATC 
GTCCTAATGC TCATTCCCCG CAATGATGCG CTTGATCAAC TGTTCAAGAC AGCTGCCGCC 
AGTCTGACAA CTATCGGAAG CCTGTTGGCC ACATGGCTCG TTTTCTTTCT GGTGTTTGCG 
ATTGCACTTA CACAGACATT TAGTCTCACC CGTTTCGGAA ATAGCACTGA TTACTATGTC 
AATCTCCGTA CGGTGCCGAA TGCCCTGATC CTACTCTTCC GGTTCAGTTG CGGAGAGGGC 
TGGAATCAGG TTATGGAAGA CTTTGCTAAG ATCAAACCGC CGCTTTGTGT GGAGAGCACC 
AATTTCTCTG ACAGCGATTG CGGCAGCACC GCATGGGCGC GTGCTCTTTT CATCGCGTGG 
AACATCATCA GCATGTACAT TTTTGTTAAC TTGTTTGTTT CGCTGATTTA CGAAAGTTTT 
TCGTACGTAT ACCAGCGGAC AAGCGGACTT GCCATGGTGG ATCGGGACGA GCTCCGGCGC 
TTCAAGGAAG CATGGCGCAG TGTTGACCCT TCAGGGACTG GATTTATCAG CAAGGAGGCT 
TTCCCGCGCC TCTTGGGTGA ACTGTCGGGC GTGTTCGAGA TGCGCATTTA TGAAAGCCAT 
GACTCGGTGC GCCAGATCCT CGAAGATGTT CGTGGTGAAG ACACGTCAAG CATCAGACAT 
GCCTCGATCA TTAGTACCAC GCAGTTTCAA ACTAGCTTGG ATCTCAAAAA GTTAAACGAA 
CGGATATCAC AAATTGATGT CGCTAAGGTG CGCGAACGCA GACGGCGGTT CAACATCTTC 
TTCGAGGAGG TGATGGTGTC TGCGGATCCA GAAAGAGGCA TTTCCTTTAC CACTGTTCTT 
ATGATTCTGG CACATTATAA GATTATTAAC GACAGCAAGA GCTTACGTCT CGAGGAATTC 
CTTCGTCGCC GCTCACGTCT TCAACGCGTT GAGGAGGAAA TACACCGTCG CGTCGTCCTC 
GGGTTCTTCG ACATGATTTA CTACTCTCGC AAGTTTCAGC GCCACATGGA GCTCAAGCGG 
TCTGCTCGGC TCACGGCGAT TCCACAGCTG GGCGTACCTG AAATTTACGT GGATAACGAT 
CATGAAAATC AAACAGACAA TGAACAGCAA AACAAGGACA GCAACAGCAA AAACAGTGCG 
AGAGCTAGCG GAAGCGGCAG CAGTACTGCT GCTGGTACAA ATTTTTTCGA ATCCACACTC 
ACGAATCCGT TCGGCTCTGG TTCCAGTACA GTCAATCCCG GCGGTGGCGC ATCCTTTCTA 
TCAGCAGACG ATGCTCGCAC AGCTCATCAC CGCAGCTGGT CTGGGGCGAG CGCCGACCTT 
TCGTCGTTTG ATACTTCATA CGGCCATCCA CTCGCCGGCC CTCGCATGAA CAAGTCGTCC 
AACTCTGTGT CGCATCGCAA CACGCATGCC TACAGCTTCG AATTGCACGA TTCCTCAGGA 
ATTGGAGGAG GAAACGCCCC TTCGCCCGGT GGCTGGGGCG GCGGGTCAGG GACGATGGAT 
AGCATGGGCT TGGGTGGCGG GAGTGCTCTG GATGATAATA ATGGCGGAAG AAGCCCCGGC 
GGCGAGGGTA CCAAGCGCGG AAGCTCCGTC AGTCCCTCGA AACTCAGCTC GATGCTGGAT 
GATTCCGTGT GGGTGGCCAG TATCAGGAGG AGCGCGACGA TGAGACGGAG CCAGAATGAT 
ACGGATTGGG GAGGAGGGTC AAGAGCTTCC GGGGGAGGAT ACTCGGGGGC TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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smco-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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