MPT5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MPT5
Entrez Gene ID	852697
Full Name	Mpt5p
Synonyms	HTR1,PUF5,UTH4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181043.1	NP_011337.1	Mpt5p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MPT5	NP_011337.1
Kluyveromyces lactis	KLLA0E13597g	XP_454563.1
Eremothecium gossypii	AGOS_ADL056W	NP_984040.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Crystal structures of Puf5p-RNA complexes reveal that the protein scaffold presents an exceptionally flat and extended interaction surface relative to other PUF proteins. In complexes with RNAs of different lengths, the protein is unchanged.
Title: RNA regulatory networks diversified through curvature of the PUF protein scaffold.

multiple Puf proteins coordinately regulate YHB1 mRNA to protect cells from nitric oxide stress.
Title: Conditional regulation of Puf1p, Puf4p, and Puf5p activity alters YHB1 mRNA stability for a rapid response to toxic nitric oxide stress in yeast.

mutations in BRR1 or MPT5 lead to a restoration of fertility and a more-or-less pronounced restoration of polarity; they also show genetic interactions with SSD1.
Title: Mutations in the Saccharomyces cerevisiae kinase Cbk1p lead to a fertility defect that can be suppressed by the absence of Brr1p or Mpt5p (Puf5p), proteins involved in RNA metabolism.

Mpt5 represses the protein levels of two pathway components, the Ste7 MAP-kinase kinase and the Tec1 transcriptional activator, and negatively regulates the kinase activity of the Kss1 MAP kinase even in the absence of stimuli.
Title: Control of signaling in a MAP-kinase pathway by an RNA-binding protein.

The following MPT5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MPT5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02075	NM_001181043.1 Latest version!	Saccharomyces cerevisiae S288C Mpt5p (MPT5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MPT5 ( NM_001181043.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011337.1 Saccharomyces cerevisiae S288C Mpt5p (MPT5), partial mRNA.

Price & Availability↑

CloneID	OSi02075
Clone ID Related Accession (Same CDS sequence)	NM_001181043.1
Accession Version	NM_001181043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Mpt5p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATCAATA ACGAACCATT TCCATCTGCC GACTCAGCAT CGATTTTAAC TACGTCTACA 
TCAAATAACT CCTTAATGTC TTACAATCAT CAGCCTCAAC TATCTATTAA CTCCGTCCAA 
TCACTCTTGG AGCCCGTGAC CCCTCCGCCT TTGGGCCAGA TGAATAACAA AAGAAACCAT 
CAAAAGGCTC ATTCGCTTGA TCTCTCTGGT TTTAATCAGT TCATATCATC GACACAATCT 
CCCTTGGCTT TGATGAATAA TACATCAACA TCGAATTCTG CTAACTCTTT TTCCCCGAAT 
CCTAATGCTG CTAGCAACTC CACTGGGCTT TCAGCCTCAA TGGCAAATCC TCCAGCCATT 
CTACCATTAA TCAATGAGTT TGATCTGGAA ATGGATGGTC CCAGGAGAAA ATCAAGCCAC 
GATTTCACGG TTGTTGCTCC TTCGAACTCT GGTGTCAATA CCTCCAGTTT AATTATGGAA 
ACACCATCCT CTTCAGTGAC TCCTGCTGCA TCTCTCAGAA ATTTTAGCAA TAGTAATAAT 
GCTGCTTCCA AATGTGGAGT GGATAATTCG TCATTTGGTT TGAGTAGCTC AACGTCTTCA 
TCTATGGTCG AAATCAGCGC ACTACCCCTT AGAGATCTGG ATTATATCAA ACTTGCCACT 
GACCAGTTTG GCTGCCGTTT TCTTCAAAAA AAATTAGAAA CCCCCAGTGA ATCCAATATG 
GTGAGAGACT TGATGTATGA ACAAATTAAG CCATTTTTCT TGGACCTTAT TTTGGATCCG 
TTCGGTAACT ATTTGGTTCA AAAACTATGC GATTATTTAA CTGCCGAGCA AAAGACATTA 
TTAATACAAA CAATATATCC AAATGTTTTC CAAATATCAA TCAATCAGTA CGGAACTCGT 
TCCTTACAGA AAATTATAGA CACTGTCGAT AACGAAGTTC AAATCGATCT CATTATTAAG 
GGATTTTCCC AAGAATTTAC TTCGATTGAG CAAGTGGTTA CTTTGATAAA CGATCTTAAT 
GGTAACCATG TGATTCAAAA GTGTATTTTC AAATTCTCGC CATCAAAATT TGGTTTCATC 
ATAGATGCTA TTGTAGAACA AAATAATATC ATTACCATTT CTACCCATAA ACATGGTTGT 
TGCGTACTAC AAAAATTACT AAGCGTTTGT ACTCTACAAC AAATTTTCAA AATTTCTGTG 
AAAATTGTGC AGTTCCTTCC TGGATTAATC AACGATCAGT TCGGTAATTA TATCATCCAA 
TTTCTGTTAG ATATCAAAGA ATTGGACTTT TACTTATTGG CTGAGTTATT TAACCGTTTA 
TCCAATGAAT TATGTCAACT ATCTTGTTTG AAGTTCTCCT CAAATGTTGT GGAAAAATTC 
ATTAAAAAAT TATTTAGAAT CATTACTGGA TTTATTGTTA ATAACAATGG GGGTGCCTCC 
CAAAGGACTG CAGTTGCTTC TGATGACGTG ATTAATGCTT CTATGAACAT TCTTTTGACT 
ACCATTGATA TATTCACAGT CAATTTAAAT GTGCTAATCA GGGATAATTT TGGTAATTAT 
GCGTTACAAA CGCTATTAGA CGTTAAGAAT TATTCTCCTC TGCTTGCTTA CAACAAAAAT 
AGTAACGCAA TTGGGCAAAA CAGCTCTAGT ACATTGAATT ACGGTAACTT TTGTAACGAT 
TTTTCATTGA AAATTGGTAA CTTGATTGTC CTTACAAAAG AATTACTTCC AAGTATTAAA 
ACTACATCCT ATGCAAAGAA AATTAAGTTG AAAGTTAAAG CTTATGCAGA AGCCACAGGT 
ATACCATTCA CTGACATATC TCCTCAAGTC ACTGCAATGA GTCATAACAA TCTTCAAACG 
ATTAACAACG AAAATAAGAA CCCCCATAAC AAAAATAGTC ATAATCATAA TCATAATCAT 
AATCATAACC ATGCTCACAA TAATAATAAC AATAATAATC AAAAGAGTCA TACCCGTCAT 
TTTTCTTTAC CAGCTAATGC TTACCATAGA AGAAGTAACA GCTCTGTAAC CAATAATTTC 
TCAAACCAAT ATGCACAAGA TCAGAAAATT CACTCTCCGC AACAAATTAT GAACTTCAAC 
CAAAACGCAT ATCCCTCGAT GGGAGCACCT TCTTTCAATT CTCAAACTAA CCCACCATTG 
GTAAGCCATA ACTCGTTACA AAACTTCGAC AACCGCCAGT TTGCAAATTT AATGGCACAT 
CCTAATTCTG CTGCACCAAT CCATTCGTTC TCATCATCTA ACATTACCAA TGTGAATCCT 
AATGTTTCAA GGGGATTTAA GCAGCCTGGA TTTATGATGA ATGAAACCGA CAAAATTAAT 
GCTAATCACT TCTCGCCATA CTCTAATGCA AATAGTCAAA ACTTCAATGA ATCTTTTGTG 
CCTCGTATGC AATATCAAAC GGAAGGTGCA AACTGGGATT CAAGTTTGTC AATGAAGTCG 
CAGCATATTG GTCAAGGCCC ATATAATCAA GTTAATATGA GCCGCAACGC TAGTATTTCC 
AATATGCCTG CCATGAATAC CGCTAGAACA TCTGATGAAC TTCAATTCAC TTTGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011337.1
CDS	1..2580
Translation	MINNEPFPSADSASILTTSTSNNSLMSYNHQPQLSINSVQSLLEPVTPPPLGQMNNKRNHQKAHSLDLSGFNQFISSTQSPLALMNNTSTSNSANSFSPNPNAASNSTGLSASMANPPAILPLINEFDLEMDGPRRKSSHDFTVVAPSNSGVNTSSLIMETPSSSVTPAASLRNFSNSNNAASKCGVDNSSFGLSSSTSSSMVEISALPLRDLDYIKLATDQFGCRFLQKKLETPSESNMVRDLMYEQIKPFFLDLILDPFGNYLVQKLCDYLTAEQKTLLIQTIYPNVFQISINQYGTRSLQKIIDTVDNEVQIDLIIKGFSQEFTSIEQVVTLINDLNGNHVIQKCIFKFSPSKFGFIIDAIVEQNNIITISTHKHGCCVLQKLLSVCTLQQIFKISVKIVQFLPGLINDQFGNYIIQFLLDIKELDFYLLAELFNRLSNELCQLSCLKFSSNVVEKFIKKLFRIITGFIVNNNGGASQRTAVASDDVINASMNILLTTIDIFTVNLNVLIRDNFGNYALQTLLDVKNYSPLLAYNKNSNAIGQNSSSTLNYGNFCNDFSLKIGNLIVLTKELLPSIKTTSYAKKIKLKVKAYAEATGIPFTDISPQVTAMSHNNLQTINNENKNPHNKNSHNHNHNHNHNHAHNNNNNNNQKSHTRHFSLPANAYHRRSNSSVTNNFSNQYAQDQKIHSPQQIMNFNQNAYPSMGAPSFNSQTNPPLVSHNSLQNFDNRQFANLMAHPNSAAPIHSFSSSNITNVNPNVSRGFKQPGFMMNETDKINANHFSPYSNANSQNFNESFVPRMQYQTEGANWDSSLSMKSQHIGQGPYNQVNMSRNASISNMPAMNTARTSDELQFTLP

Target ORF information:

RefSeq Version	NM_001181043.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Mpt5p (MPT5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181043.1

ORF Insert Sequence:

ATGATCAATA ACGAACCATT TCCATCTGCC GACTCAGCAT CGATTTTAAC TACGTCTACA 
TCAAATAACT CCTTAATGTC TTACAATCAT CAGCCTCAAC TATCTATTAA CTCCGTCCAA 
TCACTCTTGG AGCCCGTGAC CCCTCCGCCT TTGGGCCAGA TGAATAACAA AAGAAACCAT 
CAAAAGGCTC ATTCGCTTGA TCTCTCTGGT TTTAATCAGT TCATATCATC GACACAATCT 
CCCTTGGCTT TGATGAATAA TACATCAACA TCGAATTCTG CTAACTCTTT TTCCCCGAAT 
CCTAATGCTG CTAGCAACTC CACTGGGCTT TCAGCCTCAA TGGCAAATCC TCCAGCCATT 
CTACCATTAA TCAATGAGTT TGATCTGGAA ATGGATGGTC CCAGGAGAAA ATCAAGCCAC 
GATTTCACGG TTGTTGCTCC TTCGAACTCT GGTGTCAATA CCTCCAGTTT AATTATGGAA 
ACACCATCCT CTTCAGTGAC TCCTGCTGCA TCTCTCAGAA ATTTTAGCAA TAGTAATAAT 
GCTGCTTCCA AATGTGGAGT GGATAATTCG TCATTTGGTT TGAGTAGCTC AACGTCTTCA 
TCTATGGTCG AAATCAGCGC ACTACCCCTT AGAGATCTGG ATTATATCAA ACTTGCCACT 
GACCAGTTTG GCTGCCGTTT TCTTCAAAAA AAATTAGAAA CCCCCAGTGA ATCCAATATG 
GTGAGAGACT TGATGTATGA ACAAATTAAG CCATTTTTCT TGGACCTTAT TTTGGATCCG 
TTCGGTAACT ATTTGGTTCA AAAACTATGC GATTATTTAA CTGCCGAGCA AAAGACATTA 
TTAATACAAA CAATATATCC AAATGTTTTC CAAATATCAA TCAATCAGTA CGGAACTCGT 
TCCTTACAGA AAATTATAGA CACTGTCGAT AACGAAGTTC AAATCGATCT CATTATTAAG 
GGATTTTCCC AAGAATTTAC TTCGATTGAG CAAGTGGTTA CTTTGATAAA CGATCTTAAT 
GGTAACCATG TGATTCAAAA GTGTATTTTC AAATTCTCGC CATCAAAATT TGGTTTCATC 
ATAGATGCTA TTGTAGAACA AAATAATATC ATTACCATTT CTACCCATAA ACATGGTTGT 
TGCGTACTAC AAAAATTACT AAGCGTTTGT ACTCTACAAC AAATTTTCAA AATTTCTGTG 
AAAATTGTGC AGTTCCTTCC TGGATTAATC AACGATCAGT TCGGTAATTA TATCATCCAA 
TTTCTGTTAG ATATCAAAGA ATTGGACTTT TACTTATTGG CTGAGTTATT TAACCGTTTA 
TCCAATGAAT TATGTCAACT ATCTTGTTTG AAGTTCTCCT CAAATGTTGT GGAAAAATTC 
ATTAAAAAAT TATTTAGAAT CATTACTGGA TTTATTGTTA ATAACAATGG GGGTGCCTCC 
CAAAGGACTG CAGTTGCTTC TGATGACGTG ATTAATGCTT CTATGAACAT TCTTTTGACT 
ACCATTGATA TATTCACAGT CAATTTAAAT GTGCTAATCA GGGATAATTT TGGTAATTAT 
GCGTTACAAA CGCTATTAGA CGTTAAGAAT TATTCTCCTC TGCTTGCTTA CAACAAAAAT 
AGTAACGCAA TTGGGCAAAA CAGCTCTAGT ACATTGAATT ACGGTAACTT TTGTAACGAT 
TTTTCATTGA AAATTGGTAA CTTGATTGTC CTTACAAAAG AATTACTTCC AAGTATTAAA 
ACTACATCCT ATGCAAAGAA AATTAAGTTG AAAGTTAAAG CTTATGCAGA AGCCACAGGT 
ATACCATTCA CTGACATATC TCCTCAAGTC ACTGCAATGA GTCATAACAA TCTTCAAACG 
ATTAACAACG AAAATAAGAA CCCCCATAAC AAAAATAGTC ATAATCATAA TCATAATCAT 
AATCATAACC ATGCTCACAA TAATAATAAC AATAATAATC AAAAGAGTCA TACCCGTCAT 
TTTTCTTTAC CAGCTAATGC TTACCATAGA AGAAGTAACA GCTCTGTAAC CAATAATTTC 
TCAAACCAAT ATGCACAAGA TCAGAAAATT CACTCTCCGC AACAAATTAT GAACTTCAAC 
CAAAACGCAT ATCCCTCGAT GGGAGCACCT TCTTTCAATT CTCAAACTAA CCCACCATTG 
GTAAGCCATA ACTCGTTACA AAACTTCGAC AACCGCCAGT TTGCAAATTT AATGGCACAT 
CCTAATTCTG CTGCACCAAT CCATTCGTTC TCATCATCTA ACATTACCAA TGTGAATCCT 
AATGTTTCAA GGGGATTTAA GCAGCCTGGA TTTATGATGA ATGAAACCGA CAAAATTAAT 
GCTAATCACT TCTCGCCATA CTCTAATGCA AATAGTCAAA ACTTCAATGA ATCTTTTGTG 
CCTCGTATGC AATATCAAAC GGAAGGTGCA AACTGGGATT CAAGTTTGTC AATGAAGTCG 
CAGCATATTG GTCAAGGCCC ATATAATCAA GTTAATATGA GCCGCAACGC TAGTATTTCC 
AATATGCCTG CCATGAATAC CGCTAGAACA TCTGATGAAC TTCAATTCAC TTTGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MPT5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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