ECM32 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ECM32
Entrez Gene ID	856923
Full Name	Ecm32p
Synonyms	HEL1,MTT1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179066.1	NP_011103.1	RNA helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ECM32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECM32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05671	NM_001179066.1 Latest version!	Saccharomyces cerevisiae S288C RNA helicase (ECM32), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECM32 ( NM_001179066.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011103.1 Saccharomyces cerevisiae S288C RNA helicase (ECM32), partial mRNA.

Price & Availability↑

CloneID	OSi05671
Clone ID Related Accession (Same CDS sequence)	NM_001179066.1
Accession Version	NM_001179066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	RNA helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATTTTC AGTGCAGAAC GTGTAGCCAG GCTTATGATG CTGAGCAGAT GATGAAGCAT 
CTCTCAAGTA CGAGGCATAA AACTGTATTC GACACTTCAA ATGATGAAGA TATTTGCTGT 
GAGGAATGTC AAGATAAAAA TATACACCAA CTTCAAATCA TCAGGTTTGG GGGTGAAGAC 
ATGGTTTTAC TTTGTAATTC GTGTTTTCGG AAAGAATATT CAGAAACGGA GCGTCCATCA 
ACTAGCTACT CATTACAAAA CGGCTCTATA TTGAAATTTT GGGAAAAGTA CGTGAAAGTC 
AGGGAATGTT GTTGTGATGA GTGTGGGGAA GAGTCTAACT TGAATGCAAA TAGAAATGGA 
GAAGTATTAT GTGACAAGTG CTTGCCCAAA TCGAATAGAG CTAAAGACTT TGTTTCTGAA 
AAAAGTGGAA GATTCCTATA CATCTACTTG GGACTGAATG AAACACAGAA TAGCACTAGA 
AAGCCTAGAA AAAAGGGCGG CAGAAGAGTT GGTAGAGGAA AGAAGGGCAG GAAGGGTGCT 
AAGATTAAGA AGGAGAAAAA GGAAACTTTC GAGGCCAAAA TAAGTAGAAT CGCTTATGAA 
GTCAAGAAAG AAAATAGTAC CATTCAAAGC TCTAGTAGTT CCAATCTAAG AAACTTCAAA 
GGTTTCAAAG CGGTGGAAAG CGATCCAGTA GTTGCAGCAA AAGTAAGCAA ATCAGAGACT 
AGTAGAAGTA ATCCTGGCCC ATCAAATCGA AACAAAGGGA AAGGAAATAA GGCAAACCAC 
AAAAAGAATA GTGGAAATGG GATAGGAAAA GAGAAAGAGA GGAAAACAAA CATTAGAAAT 
AATGTCCGTA ACAGCCAACC TATACCAGAG GACAGAAAAA ATACCAATAG CCACGTTACA 
ACAAACTCTG GAGGGAAAGG CAAGAACGAA TCAGTGGATA AGCACCAATT ACCGCAACCA 
AAAGCTTTGA ATGGAAACGG GTCGGGCAGT ACTAATACTA CTGGTTTGAA AAAAGGGAAA 
AAAGACCATG CTGGCCAGAA GACTAAGGGC AATGATAAAA CGGGTAATAA GAATCCGCGA 
GAGGCCAAAC TAAATTCAGC TGGCAGAAAG AATGCACTTG GCAAAAAATC AAATAACCAG 
CCAAATAAGG GAACTAGCAG GTGGACAATT GGATCTGATA CTGAATCTTC TAGAGAACCT 
AGTATTAGCC CAAACGAGAA CACAACTTCA ATTACTAAAT CGCGAAACCG CAATAAGAAG 
GCTAGCAAGC CGACGCTGAA TGAAAAATCA AAAACAACAA CGATGCCAAA AAAGTTGGAG 
ACAAAAAATC AAGAGAAAAA TAATGGAAAA ACTAAGGACG GGAAACTTAT TTACGAAGAA 
GGTGAACCAC TCACAAGGTA TAATACCTTT AAGTCAACCT TATCATACCC CGATTTGAAC 
ACATACCTAA ATGATTACTC ATTTGCCCTT TTTTTGGAAC AGAAATTAGA GAACGAATTT 
GTTCAAAACT TCAACATACT ATGGCCTCGC AATGAAAAAG ATACAGCTTT TATAATCAAC 
GTTGAGAAGA ATAATAATTC AGAACTTGAG AAATTACTAC CTGCAAATTT GCTTGCACTC 
GGTAGACCGG CATTTAATGA AAGGCAACCA TTTTTCTTTT GTACTCAGGA TGAACAAAAA 
GTTTGGTATA TTTTTATCAA AGAACTTTCT ATTCAAAGGG GTAAATACGT GTTGTTAGTG 
GAGCTTTTTT CGTGGAATAA TTTGAGTTTG CCAACGAAAA ATGGTTCATC TCAATTTAAG 
CTATTGCCAA CTTCTGCGCA GACAAGTAGA ATTCTATTTG CCATGACGCG TATTACAAAC 
CCAAAATTTA TTGACCTATT ACTAGGCCAA AAGCCTATTA AAGAGATTTA TTTTGATAAC 
AGGCTAAAAT TTTCCAGTGA CAAGTTGAAC CGATCTCAGA AGACTGCAGT GGAACATGTT 
CTAAACAACA GTATCACAAT TCTACAAGGC CCACCTGGTA CAGGTAAAAC ATCAACAATA 
GAGGAGATAA TCATCCAAGT AATTGAAAGA TTTCACGCAT TCCCAATATT GTGTGTTGCC 
GCGTCAAATA TTGCTATTGA TAACATTGCC GAGAAGATTA TGGAGAATAG ACCGCAGATA 
AAGATCTTAA GAATTTTGTC AAAGAAGAAA GAACAACAGT ATAGTGATGA CCACCCATTG 
GGCGAAATTT GTCTCCACAA TATTGTATAC AAAAATCTTT CCCCAGACAT GCAAGTCGTG 
GCAAACAAAA CCCGCAGGGG CGAAATGATC TCCAAATCAG AGGACACGAA GTTTTATAAA 
GAGAAAAACC GTGTCACCAA CAAGGTCGTA TCTCAATCGC AAATAATTTT CACGACAAAC 
ATTGCTGCAG GTGGTCGTGA ATTGAAGGTT ATAAAAGAAT GTCCCGTGGT AATAATGGAT 
GAGGCTACAC AATCATCAGA GGCTTCAACA CTAGTCCCAC TTTCGTTACC AGGTATAAGA 
AATTTTGTCT TCGTTGGGGA CGAAAAGCAA TTGTCAAGTT TCAGCAACAT CCCTCAACTG 
GAAACCTCAC TATTCGAAAG AGTCCTATCT AATGGCACGT ACAAAAACCC ACTAATGCTG 
GATACGCAGT ACAGAATGCA CCCAAAAATT AGCGAATTCC CCATTAAGAA AATATACAAT 
GGTGAATTGA AGGACGGTGT CACGGACGAA CAAAAGGCAT GGCCTGGCGT GCAGCATCCG 
TTGTTTTTTT ATCAGTGCGA TTTGGGGCCC GAGAGCAGAG TGAGAAGCAC GCAAAGAGAC 
ATAGTCGGGT TTACGTACGA AAACAAACAC GAATGTGTAG AAATCGTGAA GATTATCCAA 
ATACTAATGT TGGACAAGAA GGTTCCGCTG GAAGAAATTG GTGTCATAAC GCCCTATTCT 
GCACAGCGTG ATCTGCTGTC TGACATCTTG ACCAAAAACG TAGTTATCAA CCCCAAACAG 
ATTTCTATGC AACAAGAATA CGATGAAATT GAGCTATTTA ACGCGGCTGG TTCTCAAGGA 
ACCGCCGGCA GTCTACAAAA CAATGTGATT AACATCATCA ACGGCCTACA CGTCGCGACC 
GTAGACTCCT TTCAAGGCCA CGAAAAATCT TTCATCATAT TTTCATGTGT CAGAAATAAC 
ACCGAAAACA AAATCGGCTT TTTACGTGAC AAAAGAAGGT TAAACGTGGC ATTGACAAGA 
GCCAAGCACG GACTAATTGT GGTGGGCAAC AAGAATGTCT TAAGAAAAGG AGACCCCCTA 
TGGAAAGACT ACATTACCTA CCTCGAGGAA CAAGAGGTCA TATTCACTGA TCTCACAGCT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011103.1
CDS	1..3366
Translation	MDFQCRTCSQAYDAEQMMKHLSSTRHKTVFDTSNDEDICCEECQDKNIHQLQIIRFGGEDMVLLCNSCFRKEYSETERPSTSYSLQNGSILKFWEKYVKVRECCCDECGEESNLNANRNGEVLCDKCLPKSNRAKDFVSEKSGRFLYIYLGLNETQNSTRKPRKKGGRRVGRGKKGRKGAKIKKEKKETFEAKISRIAYEVKKENSTIQSSSSSNLRNFKGFKAVESDPVVAAKVSKSETSRSNPGPSNRNKGKGNKANHKKNSGNGIGKEKERKTNIRNNVRNSQPIPEDRKNTNSHVTTNSGGKGKNESVDKHQLPQPKALNGNGSGSTNTTGLKKGKKDHAGQKTKGNDKTGNKNPREAKLNSAGRKNALGKKSNNQPNKGTSRWTIGSDTESSREPSISPNENTTSITKSRNRNKKASKPTLNEKSKTTTMPKKLETKNQEKNNGKTKDGKLIYEEGEPLTRYNTFKSTLSYPDLNTYLNDYSFALFLEQKLENEFVQNFNILWPRNEKDTAFIINVEKNNNSELEKLLPANLLALGRPAFNERQPFFFCTQDEQKVWYIFIKELSIQRGKYVLLVELFSWNNLSLPTKNGSSQFKLLPTSAQTSRILFAMTRITNPKFIDLLLGQKPIKEIYFDNRLKFSSDKLNRSQKTAVEHVLNNSITILQGPPGTGKTSTIEEIIIQVIERFHAFPILCVAASNIAIDNIAEKIMENRPQIKILRILSKKKEQQYSDDHPLGEICLHNIVYKNLSPDMQVVANKTRRGEMISKSEDTKFYKEKNRVTNKVVSQSQIIFTTNIAAGGRELKVIKECPVVIMDEATQSSEASTLVPLSLPGIRNFVFVGDEKQLSSFSNIPQLETSLFERVLSNGTYKNPLMLDTQYRMHPKISEFPIKKIYNGELKDGVTDEQKAWPGVQHPLFFYQCDLGPESRVRSTQRDIVGFTYENKHECVEIVKIIQILMLDKKVPLEEIGVITPYSAQRDLLSDILTKNVVINPKQISMQQEYDEIELFNAAGSQGTAGSLQNNVINIINGLHVATVDSFQGHEKSFIIFSCVRNNTENKIGFLRDKRRLNVALTRAKHGLIVVGNKNVLRKGDPLWKDYITYLEEQEVIFTDLTAY

Target ORF information:

RefSeq Version	NM_001179066.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C RNA helicase (ECM32), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179066.1

ORF Insert Sequence:

ATGGATTTTC AGTGCAGAAC GTGTAGCCAG GCTTATGATG CTGAGCAGAT GATGAAGCAT 
CTCTCAAGTA CGAGGCATAA AACTGTATTC GACACTTCAA ATGATGAAGA TATTTGCTGT 
GAGGAATGTC AAGATAAAAA TATACACCAA CTTCAAATCA TCAGGTTTGG GGGTGAAGAC 
ATGGTTTTAC TTTGTAATTC GTGTTTTCGG AAAGAATATT CAGAAACGGA GCGTCCATCA 
ACTAGCTACT CATTACAAAA CGGCTCTATA TTGAAATTTT GGGAAAAGTA CGTGAAAGTC 
AGGGAATGTT GTTGTGATGA GTGTGGGGAA GAGTCTAACT TGAATGCAAA TAGAAATGGA 
GAAGTATTAT GTGACAAGTG CTTGCCCAAA TCGAATAGAG CTAAAGACTT TGTTTCTGAA 
AAAAGTGGAA GATTCCTATA CATCTACTTG GGACTGAATG AAACACAGAA TAGCACTAGA 
AAGCCTAGAA AAAAGGGCGG CAGAAGAGTT GGTAGAGGAA AGAAGGGCAG GAAGGGTGCT 
AAGATTAAGA AGGAGAAAAA GGAAACTTTC GAGGCCAAAA TAAGTAGAAT CGCTTATGAA 
GTCAAGAAAG AAAATAGTAC CATTCAAAGC TCTAGTAGTT CCAATCTAAG AAACTTCAAA 
GGTTTCAAAG CGGTGGAAAG CGATCCAGTA GTTGCAGCAA AAGTAAGCAA ATCAGAGACT 
AGTAGAAGTA ATCCTGGCCC ATCAAATCGA AACAAAGGGA AAGGAAATAA GGCAAACCAC 
AAAAAGAATA GTGGAAATGG GATAGGAAAA GAGAAAGAGA GGAAAACAAA CATTAGAAAT 
AATGTCCGTA ACAGCCAACC TATACCAGAG GACAGAAAAA ATACCAATAG CCACGTTACA 
ACAAACTCTG GAGGGAAAGG CAAGAACGAA TCAGTGGATA AGCACCAATT ACCGCAACCA 
AAAGCTTTGA ATGGAAACGG GTCGGGCAGT ACTAATACTA CTGGTTTGAA AAAAGGGAAA 
AAAGACCATG CTGGCCAGAA GACTAAGGGC AATGATAAAA CGGGTAATAA GAATCCGCGA 
GAGGCCAAAC TAAATTCAGC TGGCAGAAAG AATGCACTTG GCAAAAAATC AAATAACCAG 
CCAAATAAGG GAACTAGCAG GTGGACAATT GGATCTGATA CTGAATCTTC TAGAGAACCT 
AGTATTAGCC CAAACGAGAA CACAACTTCA ATTACTAAAT CGCGAAACCG CAATAAGAAG 
GCTAGCAAGC CGACGCTGAA TGAAAAATCA AAAACAACAA CGATGCCAAA AAAGTTGGAG 
ACAAAAAATC AAGAGAAAAA TAATGGAAAA ACTAAGGACG GGAAACTTAT TTACGAAGAA 
GGTGAACCAC TCACAAGGTA TAATACCTTT AAGTCAACCT TATCATACCC CGATTTGAAC 
ACATACCTAA ATGATTACTC ATTTGCCCTT TTTTTGGAAC AGAAATTAGA GAACGAATTT 
GTTCAAAACT TCAACATACT ATGGCCTCGC AATGAAAAAG ATACAGCTTT TATAATCAAC 
GTTGAGAAGA ATAATAATTC AGAACTTGAG AAATTACTAC CTGCAAATTT GCTTGCACTC 
GGTAGACCGG CATTTAATGA AAGGCAACCA TTTTTCTTTT GTACTCAGGA TGAACAAAAA 
GTTTGGTATA TTTTTATCAA AGAACTTTCT ATTCAAAGGG GTAAATACGT GTTGTTAGTG 
GAGCTTTTTT CGTGGAATAA TTTGAGTTTG CCAACGAAAA ATGGTTCATC TCAATTTAAG 
CTATTGCCAA CTTCTGCGCA GACAAGTAGA ATTCTATTTG CCATGACGCG TATTACAAAC 
CCAAAATTTA TTGACCTATT ACTAGGCCAA AAGCCTATTA AAGAGATTTA TTTTGATAAC 
AGGCTAAAAT TTTCCAGTGA CAAGTTGAAC CGATCTCAGA AGACTGCAGT GGAACATGTT 
CTAAACAACA GTATCACAAT TCTACAAGGC CCACCTGGTA CAGGTAAAAC ATCAACAATA 
GAGGAGATAA TCATCCAAGT AATTGAAAGA TTTCACGCAT TCCCAATATT GTGTGTTGCC 
GCGTCAAATA TTGCTATTGA TAACATTGCC GAGAAGATTA TGGAGAATAG ACCGCAGATA 
AAGATCTTAA GAATTTTGTC AAAGAAGAAA GAACAACAGT ATAGTGATGA CCACCCATTG 
GGCGAAATTT GTCTCCACAA TATTGTATAC AAAAATCTTT CCCCAGACAT GCAAGTCGTG 
GCAAACAAAA CCCGCAGGGG CGAAATGATC TCCAAATCAG AGGACACGAA GTTTTATAAA 
GAGAAAAACC GTGTCACCAA CAAGGTCGTA TCTCAATCGC AAATAATTTT CACGACAAAC 
ATTGCTGCAG GTGGTCGTGA ATTGAAGGTT ATAAAAGAAT GTCCCGTGGT AATAATGGAT 
GAGGCTACAC AATCATCAGA GGCTTCAACA CTAGTCCCAC TTTCGTTACC AGGTATAAGA 
AATTTTGTCT TCGTTGGGGA CGAAAAGCAA TTGTCAAGTT TCAGCAACAT CCCTCAACTG 
GAAACCTCAC TATTCGAAAG AGTCCTATCT AATGGCACGT ACAAAAACCC ACTAATGCTG 
GATACGCAGT ACAGAATGCA CCCAAAAATT AGCGAATTCC CCATTAAGAA AATATACAAT 
GGTGAATTGA AGGACGGTGT CACGGACGAA CAAAAGGCAT GGCCTGGCGT GCAGCATCCG 
TTGTTTTTTT ATCAGTGCGA TTTGGGGCCC GAGAGCAGAG TGAGAAGCAC GCAAAGAGAC 
ATAGTCGGGT TTACGTACGA AAACAAACAC GAATGTGTAG AAATCGTGAA GATTATCCAA 
ATACTAATGT TGGACAAGAA GGTTCCGCTG GAAGAAATTG GTGTCATAAC GCCCTATTCT 
GCACAGCGTG ATCTGCTGTC TGACATCTTG ACCAAAAACG TAGTTATCAA CCCCAAACAG 
ATTTCTATGC AACAAGAATA CGATGAAATT GAGCTATTTA ACGCGGCTGG TTCTCAAGGA 
ACCGCCGGCA GTCTACAAAA CAATGTGATT AACATCATCA ACGGCCTACA CGTCGCGACC 
GTAGACTCCT TTCAAGGCCA CGAAAAATCT TTCATCATAT TTTCATGTGT CAGAAATAAC 
ACCGAAAACA AAATCGGCTT TTTACGTGAC AAAAGAAGGT TAAACGTGGC ATTGACAAGA 
GCCAAGCACG GACTAATTGT GGTGGGCAAC AAGAATGTCT TAAGAAAAGG AGACCCCCTA 
TGGAAAGACT ACATTACCTA CCTCGAGGAA CAAGAGGTCA TATTCACTGA TCTCACAGCT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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ECM32 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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