ECM32 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following ECM32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECM32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi05671 NM_001179066.1
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Saccharomyces cerevisiae S288C RNA helicase (ECM32), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi05671
    Clone ID Related Accession (Same CDS sequence) NM_001179066.1
    Accession Version NM_001179066.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product RNA helicase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGATTTTC AGTGCAGAAC GTGTAGCCAG GCTTATGATG CTGAGCAGAT GATGAAGCAT 
    CTCTCAAGTA CGAGGCATAA AACTGTATTC GACACTTCAA ATGATGAAGA TATTTGCTGT
    GAGGAATGTC AAGATAAAAA TATACACCAA CTTCAAATCA TCAGGTTTGG GGGTGAAGAC
    ATGGTTTTAC TTTGTAATTC GTGTTTTCGG AAAGAATATT CAGAAACGGA GCGTCCATCA
    ACTAGCTACT CATTACAAAA CGGCTCTATA TTGAAATTTT GGGAAAAGTA CGTGAAAGTC
    AGGGAATGTT GTTGTGATGA GTGTGGGGAA GAGTCTAACT TGAATGCAAA TAGAAATGGA
    GAAGTATTAT GTGACAAGTG CTTGCCCAAA TCGAATAGAG CTAAAGACTT TGTTTCTGAA
    AAAAGTGGAA GATTCCTATA CATCTACTTG GGACTGAATG AAACACAGAA TAGCACTAGA
    AAGCCTAGAA AAAAGGGCGG CAGAAGAGTT GGTAGAGGAA AGAAGGGCAG GAAGGGTGCT
    AAGATTAAGA AGGAGAAAAA GGAAACTTTC GAGGCCAAAA TAAGTAGAAT CGCTTATGAA
    GTCAAGAAAG AAAATAGTAC CATTCAAAGC TCTAGTAGTT CCAATCTAAG AAACTTCAAA
    GGTTTCAAAG CGGTGGAAAG CGATCCAGTA GTTGCAGCAA AAGTAAGCAA ATCAGAGACT
    AGTAGAAGTA ATCCTGGCCC ATCAAATCGA AACAAAGGGA AAGGAAATAA GGCAAACCAC
    AAAAAGAATA GTGGAAATGG GATAGGAAAA GAGAAAGAGA GGAAAACAAA CATTAGAAAT
    AATGTCCGTA ACAGCCAACC TATACCAGAG GACAGAAAAA ATACCAATAG CCACGTTACA
    ACAAACTCTG GAGGGAAAGG CAAGAACGAA TCAGTGGATA AGCACCAATT ACCGCAACCA
    AAAGCTTTGA ATGGAAACGG GTCGGGCAGT ACTAATACTA CTGGTTTGAA AAAAGGGAAA
    AAAGACCATG CTGGCCAGAA GACTAAGGGC AATGATAAAA CGGGTAATAA GAATCCGCGA
    GAGGCCAAAC TAAATTCAGC TGGCAGAAAG AATGCACTTG GCAAAAAATC AAATAACCAG
    CCAAATAAGG GAACTAGCAG GTGGACAATT GGATCTGATA CTGAATCTTC TAGAGAACCT
    AGTATTAGCC CAAACGAGAA CACAACTTCA ATTACTAAAT CGCGAAACCG CAATAAGAAG
    GCTAGCAAGC CGACGCTGAA TGAAAAATCA AAAACAACAA CGATGCCAAA AAAGTTGGAG
    ACAAAAAATC AAGAGAAAAA TAATGGAAAA ACTAAGGACG GGAAACTTAT TTACGAAGAA
    GGTGAACCAC TCACAAGGTA TAATACCTTT AAGTCAACCT TATCATACCC CGATTTGAAC
    ACATACCTAA ATGATTACTC ATTTGCCCTT TTTTTGGAAC AGAAATTAGA GAACGAATTT
    GTTCAAAACT TCAACATACT ATGGCCTCGC AATGAAAAAG ATACAGCTTT TATAATCAAC
    GTTGAGAAGA ATAATAATTC AGAACTTGAG AAATTACTAC CTGCAAATTT GCTTGCACTC
    GGTAGACCGG CATTTAATGA AAGGCAACCA TTTTTCTTTT GTACTCAGGA TGAACAAAAA
    GTTTGGTATA TTTTTATCAA AGAACTTTCT ATTCAAAGGG GTAAATACGT GTTGTTAGTG
    GAGCTTTTTT CGTGGAATAA TTTGAGTTTG CCAACGAAAA ATGGTTCATC TCAATTTAAG
    CTATTGCCAA CTTCTGCGCA GACAAGTAGA ATTCTATTTG CCATGACGCG TATTACAAAC
    CCAAAATTTA TTGACCTATT ACTAGGCCAA AAGCCTATTA AAGAGATTTA TTTTGATAAC
    AGGCTAAAAT TTTCCAGTGA CAAGTTGAAC CGATCTCAGA AGACTGCAGT GGAACATGTT
    CTAAACAACA GTATCACAAT TCTACAAGGC CCACCTGGTA CAGGTAAAAC ATCAACAATA
    GAGGAGATAA TCATCCAAGT AATTGAAAGA TTTCACGCAT TCCCAATATT GTGTGTTGCC
    GCGTCAAATA TTGCTATTGA TAACATTGCC GAGAAGATTA TGGAGAATAG ACCGCAGATA
    AAGATCTTAA GAATTTTGTC AAAGAAGAAA GAACAACAGT ATAGTGATGA CCACCCATTG
    GGCGAAATTT GTCTCCACAA TATTGTATAC AAAAATCTTT CCCCAGACAT GCAAGTCGTG
    GCAAACAAAA CCCGCAGGGG CGAAATGATC TCCAAATCAG AGGACACGAA GTTTTATAAA
    GAGAAAAACC GTGTCACCAA CAAGGTCGTA TCTCAATCGC AAATAATTTT CACGACAAAC
    ATTGCTGCAG GTGGTCGTGA ATTGAAGGTT ATAAAAGAAT GTCCCGTGGT AATAATGGAT
    GAGGCTACAC AATCATCAGA GGCTTCAACA CTAGTCCCAC TTTCGTTACC AGGTATAAGA
    AATTTTGTCT TCGTTGGGGA CGAAAAGCAA TTGTCAAGTT TCAGCAACAT CCCTCAACTG
    GAAACCTCAC TATTCGAAAG AGTCCTATCT AATGGCACGT ACAAAAACCC ACTAATGCTG
    GATACGCAGT ACAGAATGCA CCCAAAAATT AGCGAATTCC CCATTAAGAA AATATACAAT
    GGTGAATTGA AGGACGGTGT CACGGACGAA CAAAAGGCAT GGCCTGGCGT GCAGCATCCG
    TTGTTTTTTT ATCAGTGCGA TTTGGGGCCC GAGAGCAGAG TGAGAAGCAC GCAAAGAGAC
    ATAGTCGGGT TTACGTACGA AAACAAACAC GAATGTGTAG AAATCGTGAA GATTATCCAA
    ATACTAATGT TGGACAAGAA GGTTCCGCTG GAAGAAATTG GTGTCATAAC GCCCTATTCT
    GCACAGCGTG ATCTGCTGTC TGACATCTTG ACCAAAAACG TAGTTATCAA CCCCAAACAG
    ATTTCTATGC AACAAGAATA CGATGAAATT GAGCTATTTA ACGCGGCTGG TTCTCAAGGA
    ACCGCCGGCA GTCTACAAAA CAATGTGATT AACATCATCA ACGGCCTACA CGTCGCGACC
    GTAGACTCCT TTCAAGGCCA CGAAAAATCT TTCATCATAT TTTCATGTGT CAGAAATAAC
    ACCGAAAACA AAATCGGCTT TTTACGTGAC AAAAGAAGGT TAAACGTGGC ATTGACAAGA
    GCCAAGCACG GACTAATTGT GGTGGGCAAC AAGAATGTCT TAAGAAAAGG AGACCCCCTA
    TGGAAAGACT ACATTACCTA CCTCGAGGAA CAAGAGGTCA TATTCACTGA TCTCACAGCT
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_011103.1
    CDS1..3366
    Translation

    Target ORF information:

    RefSeq Version NM_001179066.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C RNA helicase (ECM32), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001179066.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGATTTTC AGTGCAGAAC GTGTAGCCAG GCTTATGATG CTGAGCAGAT GATGAAGCAT 
    CTCTCAAGTA CGAGGCATAA AACTGTATTC GACACTTCAA ATGATGAAGA TATTTGCTGT
    GAGGAATGTC AAGATAAAAA TATACACCAA CTTCAAATCA TCAGGTTTGG GGGTGAAGAC
    ATGGTTTTAC TTTGTAATTC GTGTTTTCGG AAAGAATATT CAGAAACGGA GCGTCCATCA
    ACTAGCTACT CATTACAAAA CGGCTCTATA TTGAAATTTT GGGAAAAGTA CGTGAAAGTC
    AGGGAATGTT GTTGTGATGA GTGTGGGGAA GAGTCTAACT TGAATGCAAA TAGAAATGGA
    GAAGTATTAT GTGACAAGTG CTTGCCCAAA TCGAATAGAG CTAAAGACTT TGTTTCTGAA
    AAAAGTGGAA GATTCCTATA CATCTACTTG GGACTGAATG AAACACAGAA TAGCACTAGA
    AAGCCTAGAA AAAAGGGCGG CAGAAGAGTT GGTAGAGGAA AGAAGGGCAG GAAGGGTGCT
    AAGATTAAGA AGGAGAAAAA GGAAACTTTC GAGGCCAAAA TAAGTAGAAT CGCTTATGAA
    GTCAAGAAAG AAAATAGTAC CATTCAAAGC TCTAGTAGTT CCAATCTAAG AAACTTCAAA
    GGTTTCAAAG CGGTGGAAAG CGATCCAGTA GTTGCAGCAA AAGTAAGCAA ATCAGAGACT
    AGTAGAAGTA ATCCTGGCCC ATCAAATCGA AACAAAGGGA AAGGAAATAA GGCAAACCAC
    AAAAAGAATA GTGGAAATGG GATAGGAAAA GAGAAAGAGA GGAAAACAAA CATTAGAAAT
    AATGTCCGTA ACAGCCAACC TATACCAGAG GACAGAAAAA ATACCAATAG CCACGTTACA
    ACAAACTCTG GAGGGAAAGG CAAGAACGAA TCAGTGGATA AGCACCAATT ACCGCAACCA
    AAAGCTTTGA ATGGAAACGG GTCGGGCAGT ACTAATACTA CTGGTTTGAA AAAAGGGAAA
    AAAGACCATG CTGGCCAGAA GACTAAGGGC AATGATAAAA CGGGTAATAA GAATCCGCGA
    GAGGCCAAAC TAAATTCAGC TGGCAGAAAG AATGCACTTG GCAAAAAATC AAATAACCAG
    CCAAATAAGG GAACTAGCAG GTGGACAATT GGATCTGATA CTGAATCTTC TAGAGAACCT
    AGTATTAGCC CAAACGAGAA CACAACTTCA ATTACTAAAT CGCGAAACCG CAATAAGAAG
    GCTAGCAAGC CGACGCTGAA TGAAAAATCA AAAACAACAA CGATGCCAAA AAAGTTGGAG
    ACAAAAAATC AAGAGAAAAA TAATGGAAAA ACTAAGGACG GGAAACTTAT TTACGAAGAA
    GGTGAACCAC TCACAAGGTA TAATACCTTT AAGTCAACCT TATCATACCC CGATTTGAAC
    ACATACCTAA ATGATTACTC ATTTGCCCTT TTTTTGGAAC AGAAATTAGA GAACGAATTT
    GTTCAAAACT TCAACATACT ATGGCCTCGC AATGAAAAAG ATACAGCTTT TATAATCAAC
    GTTGAGAAGA ATAATAATTC AGAACTTGAG AAATTACTAC CTGCAAATTT GCTTGCACTC
    GGTAGACCGG CATTTAATGA AAGGCAACCA TTTTTCTTTT GTACTCAGGA TGAACAAAAA
    GTTTGGTATA TTTTTATCAA AGAACTTTCT ATTCAAAGGG GTAAATACGT GTTGTTAGTG
    GAGCTTTTTT CGTGGAATAA TTTGAGTTTG CCAACGAAAA ATGGTTCATC TCAATTTAAG
    CTATTGCCAA CTTCTGCGCA GACAAGTAGA ATTCTATTTG CCATGACGCG TATTACAAAC
    CCAAAATTTA TTGACCTATT ACTAGGCCAA AAGCCTATTA AAGAGATTTA TTTTGATAAC
    AGGCTAAAAT TTTCCAGTGA CAAGTTGAAC CGATCTCAGA AGACTGCAGT GGAACATGTT
    CTAAACAACA GTATCACAAT TCTACAAGGC CCACCTGGTA CAGGTAAAAC ATCAACAATA
    GAGGAGATAA TCATCCAAGT AATTGAAAGA TTTCACGCAT TCCCAATATT GTGTGTTGCC
    GCGTCAAATA TTGCTATTGA TAACATTGCC GAGAAGATTA TGGAGAATAG ACCGCAGATA
    AAGATCTTAA GAATTTTGTC AAAGAAGAAA GAACAACAGT ATAGTGATGA CCACCCATTG
    GGCGAAATTT GTCTCCACAA TATTGTATAC AAAAATCTTT CCCCAGACAT GCAAGTCGTG
    GCAAACAAAA CCCGCAGGGG CGAAATGATC TCCAAATCAG AGGACACGAA GTTTTATAAA
    GAGAAAAACC GTGTCACCAA CAAGGTCGTA TCTCAATCGC AAATAATTTT CACGACAAAC
    ATTGCTGCAG GTGGTCGTGA ATTGAAGGTT ATAAAAGAAT GTCCCGTGGT AATAATGGAT
    GAGGCTACAC AATCATCAGA GGCTTCAACA CTAGTCCCAC TTTCGTTACC AGGTATAAGA
    AATTTTGTCT TCGTTGGGGA CGAAAAGCAA TTGTCAAGTT TCAGCAACAT CCCTCAACTG
    GAAACCTCAC TATTCGAAAG AGTCCTATCT AATGGCACGT ACAAAAACCC ACTAATGCTG
    GATACGCAGT ACAGAATGCA CCCAAAAATT AGCGAATTCC CCATTAAGAA AATATACAAT
    GGTGAATTGA AGGACGGTGT CACGGACGAA CAAAAGGCAT GGCCTGGCGT GCAGCATCCG
    TTGTTTTTTT ATCAGTGCGA TTTGGGGCCC GAGAGCAGAG TGAGAAGCAC GCAAAGAGAC
    ATAGTCGGGT TTACGTACGA AAACAAACAC GAATGTGTAG AAATCGTGAA GATTATCCAA
    ATACTAATGT TGGACAAGAA GGTTCCGCTG GAAGAAATTG GTGTCATAAC GCCCTATTCT
    GCACAGCGTG ATCTGCTGTC TGACATCTTG ACCAAAAACG TAGTTATCAA CCCCAAACAG
    ATTTCTATGC AACAAGAATA CGATGAAATT GAGCTATTTA ACGCGGCTGG TTCTCAAGGA
    ACCGCCGGCA GTCTACAAAA CAATGTGATT AACATCATCA ACGGCCTACA CGTCGCGACC
    GTAGACTCCT TTCAAGGCCA CGAAAAATCT TTCATCATAT TTTCATGTGT CAGAAATAAC
    ACCGAAAACA AAATCGGCTT TTTACGTGAC AAAAGAAGGT TAAACGTGGC ATTGACAAGA
    GCCAAGCACG GACTAATTGT GGTGGGCAAC AAGAATGTCT TAAGAAAAGG AGACCCCCTA
    TGGAAAGACT ACATTACCTA CCTCGAGGAA CAAGAGGTCA TATTCACTGA TCTCACAGCT
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
    Nature387(6632 Suppl)78-81(1997 May)
    Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG