HAA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HAA1
Entrez Gene ID	856117
Full Name	Haa1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184105.1	NP_015333.1	Haa1p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HRR25 lead to reduced Haa1 phosphorylation and increased expression of Haa1 target genes and that Hrr25 interacts with Haa1.
Title: Casein Kinase I Isoform Hrr25 Is a Negative Regulator of Haa1 in the Weak Acid Stress Response Pathway in Saccharomyces cerevisiae.

In response to acetic acid stress, marked differences were found in the regulons controlled by CgHaa1 and by its S. cerevisiae ScHaa1 ortholog, demonstrating a clear divergent evolution of the two regulatory networks. The results gathered in this study significantly advance the understanding of the molecular mechanisms underlying the success of C. glabrata as a vaginal colonizer.
Title: The CgHaa1-Regulon Mediates Response and Tolerance to Acetic Acid Stress in the Human Pathogen Candida glabrata.

These results suggest that regulation of the subcellular localization of Haa1, together with alteration of its phosphorylation status, mediates the adaptation to lactic acid stress in yeast.
Title: Nuclear localization of Haa1, which is linked to its phosphorylation status, mediates lactic acid tolerance in Saccharomyces cerevisiae.

This study demonstrated that the S. cerevisae transcription factor Haa1 requires the DNA sequence 5'-(G/C)(A/C)GG(G/C)G-3' in the promoter region of acetic-acid-responsive genes to activate their expression.
Title: Identification of a DNA-binding site for the transcription factor Haa1, required for Saccharomyces cerevisiae response to acetic acid stress.

Data suggest that the role of both HAA1 and HRK1 in providing protection against acetic acid is, at least partially, related with their involvement in the reduction of intracellular acetate concentration.
Title: Genomic expression program involving the Haa1p-regulon in Saccharomyces cerevisiae response to acetic acid.

The following HAA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HAA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04971	NM_001184105.1 Latest version!	Saccharomyces cerevisiae S288C Haa1p (HAA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HAA1 ( NM_001184105.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015333.1 Saccharomyces cerevisiae S288C Haa1p (HAA1), partial mRNA.

Price & Availability↑

CloneID	OSi04971
Clone ID Related Accession (Same CDS sequence)	NM_001184105.1
Accession Version	NM_001184105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Haa1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTCTTGA TAAATGGCAT AAAGTATGCC TGTGAGAGGT GCATAAGAGG CCATAGAGTA 
ACAACATGCA ATCATACAGA TCAACCGCTT ATGATGATCA AACCCAAAGG TAGACCTTCC 
ACTACATGCG ACTATTGTAA ACAACTTCGA AAAAACAAGA ATGCAAATCC TGAAGGTGTT 
TGCACGTGTG GCCGGCTAGA GAAGAAAAAA CTGGCACAGA AAGCCAAAGA AGAAGCAAGA 
GCTAAAGCCA AAGAAAAACA AAGAAAACAG TGTACCTGCG GGACTGATGA GGTTTGCAAA 
TATCATGCTC AAAAGAGACA TCTAAGAAAG TCCCCTTCAA GTTCTCAAAA GAAAGGAAGA 
TCCATTTCTC GTTCTCAACC AATGTTTGAA AGGGTATTGT CTTCTACTTC ACTTGACAGC 
AATATGTTAT CCGGCCACGG AGCACTATCA GATACCTCTA GCATACTGAC GAGCACATTT 
TTAGACAGTG AGCCGGGTGT TGGTAAAATT TCAAAAGATT ACCATCATGT CCCTTCATTG 
GCCTCCATTT CATCCTTACA ATCCTCGCAA TCGTTAGATC AAAATTTCAG TATACCACAA 
AGCCCGCCGT TATCTTCAAT GTCATTTAAT TTTCTCACGG GAAATATCAA TGAAACCAAC 
CAAAATCACA GTAATCATCA GCATTCAAAA TCAGGCAATA ACTGGCAAGA TAGTTCGGTA 
AGCTTGCCAG CGAAAGCTGA TTCACGTCTT AACATGATGG ATAAAAACAA CTCTGTGGGT 
CTTGACCTAT TAGGCCATTC AAAACGAATA TCGCCGATAT CAAACTCTCG TGTGGGCGAA 
GTTAGCGTTC CGCTAGAAGA ATATATTCCT TCTGACATTG ATGGGGTTGG AAGAGTTACT 
GATAAAAGCT CTTTGGTCTA CGATTGGCCA TTTGATGAAA GTATTGAGAG AAATTTCAGT 
ACAACCGCAA CCGCTGCAAC TGGTGAAAGT AAGTTCGACA TTAACGACAA CTGTAATAGA 
ATTAATAGCA AAAGTTATAG TAAGACTAAT AGTATGAATG GAAACGGTAT GAACAATAGC 
AATAATAATA ATATCAACAG TAATGGCAAC GACAAGAACA ATAACAACTC TTCTAGACAA 
GAACATCAAG GAAATGGACT ATTTGACATG TTTACAGATT CATCGTCGAT TTCAACGCTT 
TCCCGTGCAA ACTTATTATT GCAAGAAAAA ATTGGTTCGC AAGAAAACTC TGTCAAACAA 
GAAAACTATT CGAAAAATCC TCAACTTCGT CATCAATTAA CTTCCAGAAG TAGATCATTT 
ATTCATCATC CGGCAAACGA GTATTTGAAG AATACTTTTG GAAATTCACA TAGTAATGAC 
ATCGGAAAGG GAGTTGAAGT GCTATCTTTG ACACCGAGTT TTATGGATAT TCCCGAAAAA 
GAAAGAGAAA CGGAAAGATC GCCATCATCC AATTACATTA CTGACAGACC TTTCACTCGA 
AAACCTAGAT CTTCTAGCAT TGACGTAAAC CATAGGTATC CACCTATGGC ACCAACAACC 
GTAGCGACAT CTCCCGGTGC ATTGAACAAT GCCGTAGCAA GCAATCTCGA CGATCAACTG 
AGTTTAACAT CACTAAACTC TCAGCCATCA TCGATAGCAA ATATGATGAT GGACCCTTCA 
AACCTAGCTG AGCAAAGTTC TATTCATTCA GTTCCTCAGT CAATAAACTC TCCGAGAATG 
CCTAAAACTG GAAGTCGCCA AGACAAGAAC ATTCACACTA AGAAGGAAGA AAGAAATCCG 
CTAAATAACA TACACGATCT GTCACAATTG GAAAATGTAC CAGACGAGAT GAACCAAATG 
TTCTCCCCAC CATTAAAAAG TATGAATAGA CCGGATGCCA TAAGGGAAAA TTCATCTAGT 
AGTAATTTCA TAATCCAAGG AAATAGCATG ATCTCTACGC CTTCCGGAAG GAATGACCTT 
CCAGATACCT CTCCAATGAG TAGTATTCAA ACAGCGTCAC CACCAAGTCA ATTACTGACC 
GATCAAGGAT TTGCGGATTT GGATAATTTC ATGTCTTCGT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015333.1
CDS	1..2085
Translation	MVLINGIKYACERCIRGHRVTTCNHTDQPLMMIKPKGRPSTTCDYCKQLRKNKNANPEGVCTCGRLEKKKLAQKAKEEARAKAKEKQRKQCTCGTDEVCKYHAQKRHLRKSPSSSQKKGRSISRSQPMFERVLSSTSLDSNMLSGHGALSDTSSILTSTFLDSEPGVGKISKDYHHVPSLASISSLQSSQSLDQNFSIPQSPPLSSMSFNFLTGNINETNQNHSNHQHSKSGNNWQDSSVSLPAKADSRLNMMDKNNSVGLDLLGHSKRISPISNSRVGEVSVPLEEYIPSDIDGVGRVTDKSSLVYDWPFDESIERNFSTTATAATGESKFDINDNCNRINSKSYSKTNSMNGNGMNNSNNNNINSNGNDKNNNNSSRQEHQGNGLFDMFTDSSSISTLSRANLLLQEKIGSQENSVKQENYSKNPQLRHQLTSRSRSFIHHPANEYLKNTFGNSHSNDIGKGVEVLSLTPSFMDIPEKERETERSPSSNYITDRPFTRKPRSSSIDVNHRYPPMAPTTVATSPGALNNAVASNLDDQLSLTSLNSQPSSIANMMMDPSNLAEQSSIHSVPQSINSPRMPKTGSRQDKNIHTKKEERNPLNNIHDLSQLENVPDEMNQMFSPPLKSMNRPDAIRENSSSSNFIIQGNSMISTPSGRNDLPDTSPMSSIQTASPPSQLLTDQGFADLDNFMSSL

Target ORF information:

RefSeq Version	NM_001184105.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Haa1p (HAA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184105.1

ORF Insert Sequence:

ATGGTCTTGA TAAATGGCAT AAAGTATGCC TGTGAGAGGT GCATAAGAGG CCATAGAGTA 
ACAACATGCA ATCATACAGA TCAACCGCTT ATGATGATCA AACCCAAAGG TAGACCTTCC 
ACTACATGCG ACTATTGTAA ACAACTTCGA AAAAACAAGA ATGCAAATCC TGAAGGTGTT 
TGCACGTGTG GCCGGCTAGA GAAGAAAAAA CTGGCACAGA AAGCCAAAGA AGAAGCAAGA 
GCTAAAGCCA AAGAAAAACA AAGAAAACAG TGTACCTGCG GGACTGATGA GGTTTGCAAA 
TATCATGCTC AAAAGAGACA TCTAAGAAAG TCCCCTTCAA GTTCTCAAAA GAAAGGAAGA 
TCCATTTCTC GTTCTCAACC AATGTTTGAA AGGGTATTGT CTTCTACTTC ACTTGACAGC 
AATATGTTAT CCGGCCACGG AGCACTATCA GATACCTCTA GCATACTGAC GAGCACATTT 
TTAGACAGTG AGCCGGGTGT TGGTAAAATT TCAAAAGATT ACCATCATGT CCCTTCATTG 
GCCTCCATTT CATCCTTACA ATCCTCGCAA TCGTTAGATC AAAATTTCAG TATACCACAA 
AGCCCGCCGT TATCTTCAAT GTCATTTAAT TTTCTCACGG GAAATATCAA TGAAACCAAC 
CAAAATCACA GTAATCATCA GCATTCAAAA TCAGGCAATA ACTGGCAAGA TAGTTCGGTA 
AGCTTGCCAG CGAAAGCTGA TTCACGTCTT AACATGATGG ATAAAAACAA CTCTGTGGGT 
CTTGACCTAT TAGGCCATTC AAAACGAATA TCGCCGATAT CAAACTCTCG TGTGGGCGAA 
GTTAGCGTTC CGCTAGAAGA ATATATTCCT TCTGACATTG ATGGGGTTGG AAGAGTTACT 
GATAAAAGCT CTTTGGTCTA CGATTGGCCA TTTGATGAAA GTATTGAGAG AAATTTCAGT 
ACAACCGCAA CCGCTGCAAC TGGTGAAAGT AAGTTCGACA TTAACGACAA CTGTAATAGA 
ATTAATAGCA AAAGTTATAG TAAGACTAAT AGTATGAATG GAAACGGTAT GAACAATAGC 
AATAATAATA ATATCAACAG TAATGGCAAC GACAAGAACA ATAACAACTC TTCTAGACAA 
GAACATCAAG GAAATGGACT ATTTGACATG TTTACAGATT CATCGTCGAT TTCAACGCTT 
TCCCGTGCAA ACTTATTATT GCAAGAAAAA ATTGGTTCGC AAGAAAACTC TGTCAAACAA 
GAAAACTATT CGAAAAATCC TCAACTTCGT CATCAATTAA CTTCCAGAAG TAGATCATTT 
ATTCATCATC CGGCAAACGA GTATTTGAAG AATACTTTTG GAAATTCACA TAGTAATGAC 
ATCGGAAAGG GAGTTGAAGT GCTATCTTTG ACACCGAGTT TTATGGATAT TCCCGAAAAA 
GAAAGAGAAA CGGAAAGATC GCCATCATCC AATTACATTA CTGACAGACC TTTCACTCGA 
AAACCTAGAT CTTCTAGCAT TGACGTAAAC CATAGGTATC CACCTATGGC ACCAACAACC 
GTAGCGACAT CTCCCGGTGC ATTGAACAAT GCCGTAGCAA GCAATCTCGA CGATCAACTG 
AGTTTAACAT CACTAAACTC TCAGCCATCA TCGATAGCAA ATATGATGAT GGACCCTTCA 
AACCTAGCTG AGCAAAGTTC TATTCATTCA GTTCCTCAGT CAATAAACTC TCCGAGAATG 
CCTAAAACTG GAAGTCGCCA AGACAAGAAC ATTCACACTA AGAAGGAAGA AAGAAATCCG 
CTAAATAACA TACACGATCT GTCACAATTG GAAAATGTAC CAGACGAGAT GAACCAAATG 
TTCTCCCCAC CATTAAAAAG TATGAATAGA CCGGATGCCA TAAGGGAAAA TTCATCTAGT 
AGTAATTTCA TAATCCAAGG AAATAGCATG ATCTCTACGC CTTCCGGAAG GAATGACCTT 
CCAGATACCT CTCCAATGAG TAGTATTCAA ACAGCGTCAC CACCAAGTCA ATTACTGACC 
GATCAAGGAT TTGCGGATTT GGATAATTTC ATGTCTTCGT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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HAA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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