GAL4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GAL4
Entrez Gene ID	855828
Full Name	galactose-responsive transcription factor GAL4
Synonyms	GAL81
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184062.1	NP_015076.1	galactose-responsive transcription factor GAL4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

To facilitate application of the KZip(+) technology, we have developed a versatile set of LexAop-KZip(+) fly lines that can be used directly with the large number of LexA driver lines with known expression patterns. KZip(+) significantly sharpens the precision of neuronal genetic control available in Drosophila and may be extended to other organisms where Split GAL4-like systems are used.
Title: Facilitating Neuron-Specific Genetic Manipulations in

Here, the authors demonstrate that altering the epistatic interaction between Gal80p and Gal4p also abolishes the bistability of the GAL/MEL genetic switch, and the switch response to galactose becomes graded instead of binary.
Title: The binary response of the GAL/MEL genetic switch of Saccharomyces cerevisiae is critically dependent on Gal80p-Gal4p interaction.

GAL switch becomes independent of GAL3 by altering the interaction between Gal4p and Gal80p.
Title: Perturbation of the interaction between Gal4p and Gal80p of the Saccharomyces cerevisiae?GAL switch results in altered responses to galactose and glucose.

Report drosophila line in which the expression of yeast GAL4 is up-regulated in response to ingestion of silver nanoparticles
Title: Development of biomarker for detecting silver nanoparticles exposure using a GAL4 enhancer trap screening in Drosophila.

Gal4 binds to GAL gene UASGAL (upstream activation sequence in GAL gene promoter) sites as a dimer via its N-terminal domain and activates transcription via a C-terminal transcription activation domain (AD).
Title: Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch.

The following GAL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04710	NM_001184062.1 Latest version!	Saccharomyces cerevisiae S288C galactose-responsive transcription factor GAL4 (GAL4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAL4 ( NM_001184062.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015076.1 Saccharomyces cerevisiae S288C galactose-responsive transcription factor GAL4 (GAL4), partial mRNA.

Price & Availability↑

CloneID	OSi04710
Clone ID Related Accession (Same CDS sequence)	NM_001184062.1
Accession Version	NM_001184062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	galactose-responsive transcription factor GAL4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAGCTAC TGTCTTCTAT CGAACAAGCA TGCGATATTT GCCGACTTAA AAAGCTCAAG 
TGCTCCAAAG AAAAACCGAA GTGCGCCAAG TGTCTGAAGA ACAACTGGGA GTGTCGCTAC 
TCTCCCAAAA CCAAAAGGTC TCCGCTGACT AGGGCACATC TGACAGAAGT GGAATCAAGG 
CTAGAAAGAC TGGAACAGCT ATTTCTACTG ATTTTTCCTC GAGAAGACCT TGACATGATT 
TTGAAAATGG ATTCTTTACA GGATATAAAA GCATTGTTAA CAGGATTATT TGTACAAGAT 
AATGTGAATA AAGATGCCGT CACAGATAGA TTGGCTTCAG TGGAGACTGA TATGCCTCTA 
ACATTGAGAC AGCATAGAAT AAGTGCGACA TCATCATCGG AAGAGAGTAG TAACAAAGGT 
CAAAGACAGT TGACTGTATC GATTGACTCG GCAGCTCATC ATGATAACTC CACAATTCCG 
TTGGATTTTA TGCCCAGGGA TGCTCTTCAT GGATTTGATT GGTCTGAAGA GGATGACATG 
TCGGATGGCT TGCCCTTCCT GAAAACGGAC CCCAACAATA ATGGGTTCTT TGGCGACGGT 
TCTCTCTTAT GTATTCTTCG ATCTATTGGC TTTAAACCGG AAAATTACAC GAACTCTAAC 
GTTAACAGGC TCCCGACCAT GATTACGGAT AGATACACGT TGGCTTCTAG ATCCACAACA 
TCCCGTTTAC TTCAAAGTTA TCTCAATAAT TTTCACCCCT ACTGCCCTAT CGTGCACTCA 
CCGACGCTAA TGATGTTGTA TAATAACCAG ATTGAAATCG CGTCGAAGGA TCAATGGCAA 
ATCCTTTTTA ACTGCATATT AGCCATTGGA GCCTGGTGTA TAGAGGGGGA ATCTACTGAT 
ATAGATGTTT TTTACTATCA AAATGCTAAA TCTCATTTGA CGAGCAAGGT CTTCGAGTCA 
GGTTCCATAA TTTTGGTGAC AGCCCTACAT CTTCTGTCGC GATATACACA GTGGAGGCAG 
AAAACAAATA CTAGCTATAA TTTTCACAGC TTTTCCATAA GAATGGCCAT ATCATTGGGC 
TTGAATAGGG ACCTCCCCTC GTCCTTCAGT GATAGCAGCA TTCTGGAACA AAGACGCCGA 
ATTTGGTGGT CTGTCTACTC TTGGGAGATC CAATTGTCCC TGCTTTATGG TCGATCCATC 
CAGCTTTCTC AGAATACAAT CTCCTTCCCT TCTTCTGTCG ACGATGTGCA GCGTACCACA 
ACAGGTCCCA CCATATATCA TGGCATCATT GAAACAGCAA GGCTCTTACA AGTTTTCACA 
AAAATCTATG AACTAGACAA AACAGTAACT GCAGAAAAAA GTCCTATATG TGCAAAAAAA 
TGCTTGATGA TTTGTAATGA GATTGAGGAG GTTTCGAGAC AGGCACCAAA GTTTTTACAA 
ATGGATATTT CCACCACCGC TCTAACCAAT TTGTTGAAGG AACACCCTTG GCTATCCTTT 
ACAAGATTCG AACTGAAGTG GAAACAGTTG TCTCTTATCA TTTATGTATT AAGAGATTTT 
TTCACTAATT TTACCCAGAA AAAGTCACAA CTAGAACAGG ATCAAAATGA TCATCAAAGT 
TATGAAGTTA AACGATGCTC CATCATGTTA AGCGATGCAG CACAAAGAAC TGTTATGTCT 
GTAAGTAGCT ATATGGACAA TCATAATGTC ACCCCATATT TTGCCTGGAA TTGTTCTTAT 
TACTTGTTCA ATGCAGTCCT AGTACCCATA AAGACTCTAC TCTCAAACTC AAAATCGAAT 
GCTGAGAATA ACGAGACCGC ACAATTATTA CAACAAATTA ACACTGTTCT GATGCTATTA 
AAAAAACTGG CCACTTTTAA AATCCAGACT TGTGAAAAAT ACATTCAAGT ACTGGAAGAG 
GTATGTGCGC CGTTTCTGTT ATCACAGTGT GCAATCCCAT TACCGCATAT CAGTTATAAC 
AATAGTAATG GTAGCGCCAT TAAAAATATT GTCGGTTCTG CAACTATCGC CCAATACCCT 
ACTCTTCCGG AGGAAAATGT CAACAATATC AGTGTTAAAT ATGTTTCTCC TGGCTCAGTA 
GGGCCTTCAC CTGTGCCATT GAAATCAGGA GCAAGTTTCA GTGATCTAGT CAAGCTGTTA 
TCTAACCGTC CACCCTCTCG TAACTCTCCA GTGACAATAC CAAGAAGCAC ACCTTCGCAT 
CGCTCAGTCA CGCCTTTTCT AGGGCAACAG CAACAGCTGC AATCATTAGT GCCACTGACC 
CCGTCTGCTT TGTTTGGTGG CGCCAATTTT AATCAAAGTG GGAATATTGC TGATAGCTCA 
TTGTCCTTCA CTTTCACTAA CAGTAGCAAC GGTCCGAACC TCATAACAAC TCAAACAAAT 
TCTCAAGCGC TTTCACAACC AATTGCCTCC TCTAACGTTC ATGATAACTT CATGAATAAT 
GAAATCACGG CTAGTAAAAT TGATGATGGT AATAATTCAA AACCACTGTC ACCTGGTTGG 
ACGGACCAAA CTGCGTATAA CGCGTTTGGA ATCACTACAG GGATGTTTAA TACCACTACA 
ATGGATGATG TATATAACTA TCTATTCGAT GATGAAGATA CCCCACCAAA CCCAAAAAAA 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015076.1
CDS	1..2646
Translation	MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDFMPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVNRLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQILFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQKTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRSIQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICAKKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVLRDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAWNCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYIQVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKYVSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQLQSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE

Target ORF information:

RefSeq Version	NM_001184062.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C galactose-responsive transcription factor GAL4 (GAL4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184062.1

ORF Insert Sequence:

ATGAAGCTAC TGTCTTCTAT CGAACAAGCA TGCGATATTT GCCGACTTAA AAAGCTCAAG 
TGCTCCAAAG AAAAACCGAA GTGCGCCAAG TGTCTGAAGA ACAACTGGGA GTGTCGCTAC 
TCTCCCAAAA CCAAAAGGTC TCCGCTGACT AGGGCACATC TGACAGAAGT GGAATCAAGG 
CTAGAAAGAC TGGAACAGCT ATTTCTACTG ATTTTTCCTC GAGAAGACCT TGACATGATT 
TTGAAAATGG ATTCTTTACA GGATATAAAA GCATTGTTAA CAGGATTATT TGTACAAGAT 
AATGTGAATA AAGATGCCGT CACAGATAGA TTGGCTTCAG TGGAGACTGA TATGCCTCTA 
ACATTGAGAC AGCATAGAAT AAGTGCGACA TCATCATCGG AAGAGAGTAG TAACAAAGGT 
CAAAGACAGT TGACTGTATC GATTGACTCG GCAGCTCATC ATGATAACTC CACAATTCCG 
TTGGATTTTA TGCCCAGGGA TGCTCTTCAT GGATTTGATT GGTCTGAAGA GGATGACATG 
TCGGATGGCT TGCCCTTCCT GAAAACGGAC CCCAACAATA ATGGGTTCTT TGGCGACGGT 
TCTCTCTTAT GTATTCTTCG ATCTATTGGC TTTAAACCGG AAAATTACAC GAACTCTAAC 
GTTAACAGGC TCCCGACCAT GATTACGGAT AGATACACGT TGGCTTCTAG ATCCACAACA 
TCCCGTTTAC TTCAAAGTTA TCTCAATAAT TTTCACCCCT ACTGCCCTAT CGTGCACTCA 
CCGACGCTAA TGATGTTGTA TAATAACCAG ATTGAAATCG CGTCGAAGGA TCAATGGCAA 
ATCCTTTTTA ACTGCATATT AGCCATTGGA GCCTGGTGTA TAGAGGGGGA ATCTACTGAT 
ATAGATGTTT TTTACTATCA AAATGCTAAA TCTCATTTGA CGAGCAAGGT CTTCGAGTCA 
GGTTCCATAA TTTTGGTGAC AGCCCTACAT CTTCTGTCGC GATATACACA GTGGAGGCAG 
AAAACAAATA CTAGCTATAA TTTTCACAGC TTTTCCATAA GAATGGCCAT ATCATTGGGC 
TTGAATAGGG ACCTCCCCTC GTCCTTCAGT GATAGCAGCA TTCTGGAACA AAGACGCCGA 
ATTTGGTGGT CTGTCTACTC TTGGGAGATC CAATTGTCCC TGCTTTATGG TCGATCCATC 
CAGCTTTCTC AGAATACAAT CTCCTTCCCT TCTTCTGTCG ACGATGTGCA GCGTACCACA 
ACAGGTCCCA CCATATATCA TGGCATCATT GAAACAGCAA GGCTCTTACA AGTTTTCACA 
AAAATCTATG AACTAGACAA AACAGTAACT GCAGAAAAAA GTCCTATATG TGCAAAAAAA 
TGCTTGATGA TTTGTAATGA GATTGAGGAG GTTTCGAGAC AGGCACCAAA GTTTTTACAA 
ATGGATATTT CCACCACCGC TCTAACCAAT TTGTTGAAGG AACACCCTTG GCTATCCTTT 
ACAAGATTCG AACTGAAGTG GAAACAGTTG TCTCTTATCA TTTATGTATT AAGAGATTTT 
TTCACTAATT TTACCCAGAA AAAGTCACAA CTAGAACAGG ATCAAAATGA TCATCAAAGT 
TATGAAGTTA AACGATGCTC CATCATGTTA AGCGATGCAG CACAAAGAAC TGTTATGTCT 
GTAAGTAGCT ATATGGACAA TCATAATGTC ACCCCATATT TTGCCTGGAA TTGTTCTTAT 
TACTTGTTCA ATGCAGTCCT AGTACCCATA AAGACTCTAC TCTCAAACTC AAAATCGAAT 
GCTGAGAATA ACGAGACCGC ACAATTATTA CAACAAATTA ACACTGTTCT GATGCTATTA 
AAAAAACTGG CCACTTTTAA AATCCAGACT TGTGAAAAAT ACATTCAAGT ACTGGAAGAG 
GTATGTGCGC CGTTTCTGTT ATCACAGTGT GCAATCCCAT TACCGCATAT CAGTTATAAC 
AATAGTAATG GTAGCGCCAT TAAAAATATT GTCGGTTCTG CAACTATCGC CCAATACCCT 
ACTCTTCCGG AGGAAAATGT CAACAATATC AGTGTTAAAT ATGTTTCTCC TGGCTCAGTA 
GGGCCTTCAC CTGTGCCATT GAAATCAGGA GCAAGTTTCA GTGATCTAGT CAAGCTGTTA 
TCTAACCGTC CACCCTCTCG TAACTCTCCA GTGACAATAC CAAGAAGCAC ACCTTCGCAT 
CGCTCAGTCA CGCCTTTTCT AGGGCAACAG CAACAGCTGC AATCATTAGT GCCACTGACC 
CCGTCTGCTT TGTTTGGTGG CGCCAATTTT AATCAAAGTG GGAATATTGC TGATAGCTCA 
TTGTCCTTCA CTTTCACTAA CAGTAGCAAC GGTCCGAACC TCATAACAAC TCAAACAAAT 
TCTCAAGCGC TTTCACAACC AATTGCCTCC TCTAACGTTC ATGATAACTT CATGAATAAT 
GAAATCACGG CTAGTAAAAT TGATGATGGT AATAATTCAA AACCACTGTC ACCTGGTTGG 
ACGGACCAAA CTGCGTATAA CGCGTTTGGA ATCACTACAG GGATGTTTAA TACCACTACA 
ATGGATGATG TATATAACTA TCTATTCGAT GATGAAGATA CCCCACCAAA CCCAAAAAAA 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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GAL4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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