PFK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following PFK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02455 NM_001181369.1
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Saccharomyces cerevisiae S288C 6-phosphofructokinase subunit alpha (PFK1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi02455
    Clone ID Related Accession (Same CDS sequence) NM_001181369.1
    Accession Version NM_001181369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product 6-phosphofructokinase subunit alpha
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAATCTC AAGATTCATG CTACGGTGTT GCATTCAGAT CTATCATCAC AAATGATGAA 
    GCTTTATTCA AGAAGACCAT TCACTTTTAT CACACTCTAG GATTTGCAAC TGTGAAAGAT
    TTCAACAAAT TCAAACATGG TGAAAATAGC TTACTATCTT CAGGGACTTC CCAAGATTCC
    TTGAGAGAAG TTTGGTTAGA ATCTTTCAAG TTGAGTGAGG TTGATGCTTC TGGGTTCCGT
    ATACCACAAC AAGAAGCTAC TAACAAGGCT CAAAGTCAAG GTGCTCTATT AAAGATTCGT
    TTAGTGATGT CTGCTCCAAT CGATGAAACT TTCGACACCA ACGAAACCGC CACAATCACT
    TATTTCTCTA CTGATTTGAA CAAGATTGTC GAGAAATTCC CAAAACAAGC CGAAAAATTG
    TCCGATACCT TAGTGTTTTT GAAAGATCCA ATGGGCAACA ACATCACCTT CTCAGGCTTA
    GCTAATGCAA CCGATTCCGC TCCAACTTCC AAAGATGCTT TCTTAGAAGC TACCTCCGAA
    GACGAAATCA TCTCTAGAGC TTCTTCCGAT GCTTCTGACT TACTAAGACA AACATTGGGC
    TCTTCTCAAA AGAAGAAGAA GATTGCTGTC ATGACTTCTG GTGGTGATTC TCCAGGTATG
    AATGCCGCTG TTCGTGCCGT TGTTCGTACA GGTATACATT TCGGCTGTGA TGTTTTTGCT
    GTTTACGAAG GTTACGAAGG TTTACTAAGA GGCGGTAAAT ATTTAAAGAA AATGGCTTGG
    GAAGATGTCA GAGGTTGGTT AAGTGAAGGT GGTACTTTGA TTGGTACTGC TCGTTCTATG
    GAATTCAGAA AGCGTGAGGG TCGTAGACAA GCTGCAGGCA ATTTAATTTC GCAAGGTATT
    GACGCTTTGG TTGTTTGTGG TGGTGATGGT TCTTTAACCG GTGCTGATCT TTTCAGACAC
    GAATGGCCAT CTTTGGTTGA TGAATTGGTT GCAGAAGGTA GATTCACTAA AGAAGAAGTC
    GCCCCATACA AGAATTTGTC CATTGTTGGT CTTGTCGGTT CCATCGATAA TGATATGTCT
    GGTACTGACT CTACCATTGG TGCTTATTCT GCTTTGGAAA GAATCTGTGA AATGGTTGAC
    TACATTGATG CCACCGCTAA ATCCCACTCC CGTGCCTTTG TTGTTGAAGT TATGGGTAGA
    CATTGTGGTT GGTTGGCCTT GATGGCTGGT ATTGCTACCG GTGCCGATTA CATTTTTATT
    CCAGAAAGAG CTGTTCCTCA CGGAAAATGG CAGGACGAAT TGAAGGAAGT GTGCCAAAGA
    CACAGAAGTA AGGGTAGAAG AAATAACACA ATTATTGTCG CTGAAGGTGC TTTAGATGAT
    CAATTAAACC CTGTTACTGC CAATGACGTC AAAGATGCTT TGATTGAATT GGGTCTAGAC
    ACCAAGGTAA CCATTCTAGG TCACGTTCAA AGAGGTGGTA CAGCTGTTGC TCATGACAGA
    TGGTTAGCTA CTCTACAAGG TGTCGATGCT GTTAAGGCCG TTCTGGAATT TACCCCTGAA
    ACTCCTTCTC CATTAATTGG TATTTTAGAA AACAAGATAA TTAGAATGCC ATTGGTTGAA
    TCTGTGAAGT TGACTAAATC TGTTGCCACT GCCATTGAAA ACAAAGATTT CGATAAGGCA
    ATTTCTTTAA GAGACACAGA ATTTATTGAA CTTTACGAAA ACTTCTTATC CACTACCGTT
    AAAGATGATG GTTCCGAATT ATTGCCAGTA TCTGACAGAC TAAACATTGG TATTGTCCAT
    GTTGGTGCCC CATCTGCTGC TTTGAACGCT GCCACCCGTG CCGCAACTCT ATACTGTTTG
    TCTCACGGCC ATAAACCATA CGCTATCATG AATGGTTTCA GTGGATTGAT TCAAACCGGT
    GAAGTGAAGG AACTATCATG GATTGATGTC GAAAACTGGC ATAACTTGGG TGGTTCCGAA
    ATCGGTACGA ACAGATCTGT TGCTTCAGAA GATTTGGGTA CCATTGCTTA CTACTTCCAA
    AAGAACAAGC TAGACGGTTT GATTATTCTT GGTGGTTTTG AAGGTTTCAG GTCCTTGAAG
    CAATTGCGTG ACGGTAGAAC CCAACACCCA ATCTTTAACA TTCCAATGTG TTTGATTCCA
    GCCACTGTTT CTAACAACGT TCCAGGTACT GAATACTCAC TTGGTGTTGA TACCTGTTTG
    AACGCATTAG TCAATTACAC TGATGACATC AAACAGAGTG CTTCTGCGAC AAGAAGAAGA
    GTCTTCGTCT GTGAAGTCCA AGGTGGTCAC TCTGGTTACA TCGCTTCTTT CACTGGTTTA
    ATCACTGGTG CTGTTTCAGT GTACACTCCA GAAAAGAAGA TCGACTTAGC TTCTATCAGA
    GAAGATATAA CTCTATTAAA AGAGAACTTT CGTCACGACA AAGGTGAAAA CAGAAACGGT
    AAGCTATTGG TTAGAAACGA ACAAGCTTCT AGCGTATATA GCACTCAATT GTTGGCTGAC
    ATCATCTCTG AAGCAAGCAA GGGTAAGTTT GGTGTTAGAA CTGCTATCCC AGGCCATGTT
    CAACAAGGTG GTGTTCCATC TTCTAAAGAC CGTGTCACCG CTTCCAGATT TGCTGTCAAA
    TGTATCAAGT TTATCGAACA ATGGAACAAG AAAAATGAAG CTTCTCCAAA CACTGACGCT
    AAGGTTTTGA GATTCAAGTT CGATACTCAC GGTGAAAAGG TACCAACTGT TGAGCACGAA
    GATGACTCTG CTGCTGTTAT CTGTGTTAAT GGTTCTCACG TTTCCTTCAA GCCAATTGCT
    AACCTTTGGG AAAACGAAAC CAACGTTGAA TTAAGAAAGG GTTTTGAAGT TCACTGGGCT
    GAATACAACA AGATTGGTGA CATCCTGTCC GGTAGATTAA AGTTGAGAGC TGAGGTAGCC
    GCTTTAGCCG CTGAAAACAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_011756.1
    CDS1..2964
    Translation

    Target ORF information:

    RefSeq Version NM_001181369.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C 6-phosphofructokinase subunit alpha (PFK1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001181369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAATCTC AAGATTCATG CTACGGTGTT GCATTCAGAT CTATCATCAC AAATGATGAA 
    GCTTTATTCA AGAAGACCAT TCACTTTTAT CACACTCTAG GATTTGCAAC TGTGAAAGAT
    TTCAACAAAT TCAAACATGG TGAAAATAGC TTACTATCTT CAGGGACTTC CCAAGATTCC
    TTGAGAGAAG TTTGGTTAGA ATCTTTCAAG TTGAGTGAGG TTGATGCTTC TGGGTTCCGT
    ATACCACAAC AAGAAGCTAC TAACAAGGCT CAAAGTCAAG GTGCTCTATT AAAGATTCGT
    TTAGTGATGT CTGCTCCAAT CGATGAAACT TTCGACACCA ACGAAACCGC CACAATCACT
    TATTTCTCTA CTGATTTGAA CAAGATTGTC GAGAAATTCC CAAAACAAGC CGAAAAATTG
    TCCGATACCT TAGTGTTTTT GAAAGATCCA ATGGGCAACA ACATCACCTT CTCAGGCTTA
    GCTAATGCAA CCGATTCCGC TCCAACTTCC AAAGATGCTT TCTTAGAAGC TACCTCCGAA
    GACGAAATCA TCTCTAGAGC TTCTTCCGAT GCTTCTGACT TACTAAGACA AACATTGGGC
    TCTTCTCAAA AGAAGAAGAA GATTGCTGTC ATGACTTCTG GTGGTGATTC TCCAGGTATG
    AATGCCGCTG TTCGTGCCGT TGTTCGTACA GGTATACATT TCGGCTGTGA TGTTTTTGCT
    GTTTACGAAG GTTACGAAGG TTTACTAAGA GGCGGTAAAT ATTTAAAGAA AATGGCTTGG
    GAAGATGTCA GAGGTTGGTT AAGTGAAGGT GGTACTTTGA TTGGTACTGC TCGTTCTATG
    GAATTCAGAA AGCGTGAGGG TCGTAGACAA GCTGCAGGCA ATTTAATTTC GCAAGGTATT
    GACGCTTTGG TTGTTTGTGG TGGTGATGGT TCTTTAACCG GTGCTGATCT TTTCAGACAC
    GAATGGCCAT CTTTGGTTGA TGAATTGGTT GCAGAAGGTA GATTCACTAA AGAAGAAGTC
    GCCCCATACA AGAATTTGTC CATTGTTGGT CTTGTCGGTT CCATCGATAA TGATATGTCT
    GGTACTGACT CTACCATTGG TGCTTATTCT GCTTTGGAAA GAATCTGTGA AATGGTTGAC
    TACATTGATG CCACCGCTAA ATCCCACTCC CGTGCCTTTG TTGTTGAAGT TATGGGTAGA
    CATTGTGGTT GGTTGGCCTT GATGGCTGGT ATTGCTACCG GTGCCGATTA CATTTTTATT
    CCAGAAAGAG CTGTTCCTCA CGGAAAATGG CAGGACGAAT TGAAGGAAGT GTGCCAAAGA
    CACAGAAGTA AGGGTAGAAG AAATAACACA ATTATTGTCG CTGAAGGTGC TTTAGATGAT
    CAATTAAACC CTGTTACTGC CAATGACGTC AAAGATGCTT TGATTGAATT GGGTCTAGAC
    ACCAAGGTAA CCATTCTAGG TCACGTTCAA AGAGGTGGTA CAGCTGTTGC TCATGACAGA
    TGGTTAGCTA CTCTACAAGG TGTCGATGCT GTTAAGGCCG TTCTGGAATT TACCCCTGAA
    ACTCCTTCTC CATTAATTGG TATTTTAGAA AACAAGATAA TTAGAATGCC ATTGGTTGAA
    TCTGTGAAGT TGACTAAATC TGTTGCCACT GCCATTGAAA ACAAAGATTT CGATAAGGCA
    ATTTCTTTAA GAGACACAGA ATTTATTGAA CTTTACGAAA ACTTCTTATC CACTACCGTT
    AAAGATGATG GTTCCGAATT ATTGCCAGTA TCTGACAGAC TAAACATTGG TATTGTCCAT
    GTTGGTGCCC CATCTGCTGC TTTGAACGCT GCCACCCGTG CCGCAACTCT ATACTGTTTG
    TCTCACGGCC ATAAACCATA CGCTATCATG AATGGTTTCA GTGGATTGAT TCAAACCGGT
    GAAGTGAAGG AACTATCATG GATTGATGTC GAAAACTGGC ATAACTTGGG TGGTTCCGAA
    ATCGGTACGA ACAGATCTGT TGCTTCAGAA GATTTGGGTA CCATTGCTTA CTACTTCCAA
    AAGAACAAGC TAGACGGTTT GATTATTCTT GGTGGTTTTG AAGGTTTCAG GTCCTTGAAG
    CAATTGCGTG ACGGTAGAAC CCAACACCCA ATCTTTAACA TTCCAATGTG TTTGATTCCA
    GCCACTGTTT CTAACAACGT TCCAGGTACT GAATACTCAC TTGGTGTTGA TACCTGTTTG
    AACGCATTAG TCAATTACAC TGATGACATC AAACAGAGTG CTTCTGCGAC AAGAAGAAGA
    GTCTTCGTCT GTGAAGTCCA AGGTGGTCAC TCTGGTTACA TCGCTTCTTT CACTGGTTTA
    ATCACTGGTG CTGTTTCAGT GTACACTCCA GAAAAGAAGA TCGACTTAGC TTCTATCAGA
    GAAGATATAA CTCTATTAAA AGAGAACTTT CGTCACGACA AAGGTGAAAA CAGAAACGGT
    AAGCTATTGG TTAGAAACGA ACAAGCTTCT AGCGTATATA GCACTCAATT GTTGGCTGAC
    ATCATCTCTG AAGCAAGCAA GGGTAAGTTT GGTGTTAGAA CTGCTATCCC AGGCCATGTT
    CAACAAGGTG GTGTTCCATC TTCTAAAGAC CGTGTCACCG CTTCCAGATT TGCTGTCAAA
    TGTATCAAGT TTATCGAACA ATGGAACAAG AAAAATGAAG CTTCTCCAAA CACTGACGCT
    AAGGTTTTGA GATTCAAGTT CGATACTCAC GGTGAAAAGG TACCAACTGT TGAGCACGAA
    GATGACTCTG CTGCTGTTAT CTGTGTTAAT GGTTCTCACG TTTCCTTCAA GCCAATTGCT
    AACCTTTGGG AAAACGAAAC CAACGTTGAA TTAAGAAAGG GTTTTGAAGT TCACTGGGCT
    GAATACAACA AGATTGGTGA CATCCTGTCC GGTAGATTAA AGTTGAGAGC TGAGGTAGCC
    GCTTTAGCCG CTGAAAACAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
    Nature387(6632 Suppl)81-4(1997 May)
    Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG