PFK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PFK1
Entrez Gene ID	853155
Full Name	6-phosphofructokinase subunit alpha
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181369.1	NP_011756.1	6-phosphofructokinase subunit alpha

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pfkp	NP_062677.1
Danio rerio (zebrafish)	pfkpb	XP_005163618.1
Saccharomyces cerevisiae (baker's yeast)	PFK1	NP_011756.1
Homo sapiens (human)	PFKP	NP_002618.1
Danio rerio (zebrafish)	pfkpa	XP_005162583.1
Canis lupus familiaris (dog)	PFKP	XP_005617243.1
Bos taurus (cattle)	PFKP	NP_001180149.1
Rattus norvegicus (Norway rat)	Pfkp	NP_996729.1
Kluyveromyces lactis	KLLA0A05544g	XP_451245.1
Eremothecium gossypii	AGOS_AEL208W	NP_984653.1
Xenopus tropicalis (tropical clawed frog)	pfkp	NP_989217.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Regulation of Vacuolar H+-ATPase (V-ATPase) Reassembly by Glycolysis Flow in 6-Phosphofructo-1-kinase (PFK-1)-deficient Yeast Cells.
Title: Regulation of Vacuolar H+-ATPase (V-ATPase) Reassembly by Glycolysis Flow in 6-Phosphofructo-1-kinase (PFK-1)-deficient Yeast Cells.

stability and function of the metabolon composed of H+ATPase and glycolytic components can be compromised by either loss of required PFK-1 binding (G820R) or loss of pump protein (R807Q)
Title: Human H+ATPase a4 subunit mutations causing renal tubular acidosis reveal a role for interaction with phosphofructokinase-1.

Pfk1 underwent a large conformational change upon Mg-ATP binding arising from a reorientation of the alpha- and beta-subunits in the dimers.
Title: The structure of the ATP-bound state of S. cerevisiae phosphofructokinase determined by cryo-electron microscopy.

The following PFK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02455	NM_001181369.1 Latest version!	Saccharomyces cerevisiae S288C 6-phosphofructokinase subunit alpha (PFK1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFK1 ( NM_001181369.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011756.1 Saccharomyces cerevisiae S288C 6-phosphofructokinase subunit alpha (PFK1), partial mRNA.

Price & Availability↑

CloneID	OSi02455
Clone ID Related Accession (Same CDS sequence)	NM_001181369.1
Accession Version	NM_001181369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	6-phosphofructokinase subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCAATCTC AAGATTCATG CTACGGTGTT GCATTCAGAT CTATCATCAC AAATGATGAA 
GCTTTATTCA AGAAGACCAT TCACTTTTAT CACACTCTAG GATTTGCAAC TGTGAAAGAT 
TTCAACAAAT TCAAACATGG TGAAAATAGC TTACTATCTT CAGGGACTTC CCAAGATTCC 
TTGAGAGAAG TTTGGTTAGA ATCTTTCAAG TTGAGTGAGG TTGATGCTTC TGGGTTCCGT 
ATACCACAAC AAGAAGCTAC TAACAAGGCT CAAAGTCAAG GTGCTCTATT AAAGATTCGT 
TTAGTGATGT CTGCTCCAAT CGATGAAACT TTCGACACCA ACGAAACCGC CACAATCACT 
TATTTCTCTA CTGATTTGAA CAAGATTGTC GAGAAATTCC CAAAACAAGC CGAAAAATTG 
TCCGATACCT TAGTGTTTTT GAAAGATCCA ATGGGCAACA ACATCACCTT CTCAGGCTTA 
GCTAATGCAA CCGATTCCGC TCCAACTTCC AAAGATGCTT TCTTAGAAGC TACCTCCGAA 
GACGAAATCA TCTCTAGAGC TTCTTCCGAT GCTTCTGACT TACTAAGACA AACATTGGGC 
TCTTCTCAAA AGAAGAAGAA GATTGCTGTC ATGACTTCTG GTGGTGATTC TCCAGGTATG 
AATGCCGCTG TTCGTGCCGT TGTTCGTACA GGTATACATT TCGGCTGTGA TGTTTTTGCT 
GTTTACGAAG GTTACGAAGG TTTACTAAGA GGCGGTAAAT ATTTAAAGAA AATGGCTTGG 
GAAGATGTCA GAGGTTGGTT AAGTGAAGGT GGTACTTTGA TTGGTACTGC TCGTTCTATG 
GAATTCAGAA AGCGTGAGGG TCGTAGACAA GCTGCAGGCA ATTTAATTTC GCAAGGTATT 
GACGCTTTGG TTGTTTGTGG TGGTGATGGT TCTTTAACCG GTGCTGATCT TTTCAGACAC 
GAATGGCCAT CTTTGGTTGA TGAATTGGTT GCAGAAGGTA GATTCACTAA AGAAGAAGTC 
GCCCCATACA AGAATTTGTC CATTGTTGGT CTTGTCGGTT CCATCGATAA TGATATGTCT 
GGTACTGACT CTACCATTGG TGCTTATTCT GCTTTGGAAA GAATCTGTGA AATGGTTGAC 
TACATTGATG CCACCGCTAA ATCCCACTCC CGTGCCTTTG TTGTTGAAGT TATGGGTAGA 
CATTGTGGTT GGTTGGCCTT GATGGCTGGT ATTGCTACCG GTGCCGATTA CATTTTTATT 
CCAGAAAGAG CTGTTCCTCA CGGAAAATGG CAGGACGAAT TGAAGGAAGT GTGCCAAAGA 
CACAGAAGTA AGGGTAGAAG AAATAACACA ATTATTGTCG CTGAAGGTGC TTTAGATGAT 
CAATTAAACC CTGTTACTGC CAATGACGTC AAAGATGCTT TGATTGAATT GGGTCTAGAC 
ACCAAGGTAA CCATTCTAGG TCACGTTCAA AGAGGTGGTA CAGCTGTTGC TCATGACAGA 
TGGTTAGCTA CTCTACAAGG TGTCGATGCT GTTAAGGCCG TTCTGGAATT TACCCCTGAA 
ACTCCTTCTC CATTAATTGG TATTTTAGAA AACAAGATAA TTAGAATGCC ATTGGTTGAA 
TCTGTGAAGT TGACTAAATC TGTTGCCACT GCCATTGAAA ACAAAGATTT CGATAAGGCA 
ATTTCTTTAA GAGACACAGA ATTTATTGAA CTTTACGAAA ACTTCTTATC CACTACCGTT 
AAAGATGATG GTTCCGAATT ATTGCCAGTA TCTGACAGAC TAAACATTGG TATTGTCCAT 
GTTGGTGCCC CATCTGCTGC TTTGAACGCT GCCACCCGTG CCGCAACTCT ATACTGTTTG 
TCTCACGGCC ATAAACCATA CGCTATCATG AATGGTTTCA GTGGATTGAT TCAAACCGGT 
GAAGTGAAGG AACTATCATG GATTGATGTC GAAAACTGGC ATAACTTGGG TGGTTCCGAA 
ATCGGTACGA ACAGATCTGT TGCTTCAGAA GATTTGGGTA CCATTGCTTA CTACTTCCAA 
AAGAACAAGC TAGACGGTTT GATTATTCTT GGTGGTTTTG AAGGTTTCAG GTCCTTGAAG 
CAATTGCGTG ACGGTAGAAC CCAACACCCA ATCTTTAACA TTCCAATGTG TTTGATTCCA 
GCCACTGTTT CTAACAACGT TCCAGGTACT GAATACTCAC TTGGTGTTGA TACCTGTTTG 
AACGCATTAG TCAATTACAC TGATGACATC AAACAGAGTG CTTCTGCGAC AAGAAGAAGA 
GTCTTCGTCT GTGAAGTCCA AGGTGGTCAC TCTGGTTACA TCGCTTCTTT CACTGGTTTA 
ATCACTGGTG CTGTTTCAGT GTACACTCCA GAAAAGAAGA TCGACTTAGC TTCTATCAGA 
GAAGATATAA CTCTATTAAA AGAGAACTTT CGTCACGACA AAGGTGAAAA CAGAAACGGT 
AAGCTATTGG TTAGAAACGA ACAAGCTTCT AGCGTATATA GCACTCAATT GTTGGCTGAC 
ATCATCTCTG AAGCAAGCAA GGGTAAGTTT GGTGTTAGAA CTGCTATCCC AGGCCATGTT 
CAACAAGGTG GTGTTCCATC TTCTAAAGAC CGTGTCACCG CTTCCAGATT TGCTGTCAAA 
TGTATCAAGT TTATCGAACA ATGGAACAAG AAAAATGAAG CTTCTCCAAA CACTGACGCT 
AAGGTTTTGA GATTCAAGTT CGATACTCAC GGTGAAAAGG TACCAACTGT TGAGCACGAA 
GATGACTCTG CTGCTGTTAT CTGTGTTAAT GGTTCTCACG TTTCCTTCAA GCCAATTGCT 
AACCTTTGGG AAAACGAAAC CAACGTTGAA TTAAGAAAGG GTTTTGAAGT TCACTGGGCT 
GAATACAACA AGATTGGTGA CATCCTGTCC GGTAGATTAA AGTTGAGAGC TGAGGTAGCC 
GCTTTAGCCG CTGAAAACAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011756.1
CDS	1..2964
Translation	MQSQDSCYGVAFRSIITNDEALFKKTIHFYHTLGFATVKDFNKFKHGENSLLSSGTSQDSLREVWLESFKLSEVDASGFRIPQQEATNKAQSQGALLKIRLVMSAPIDETFDTNETATITYFSTDLNKIVEKFPKQAEKLSDTLVFLKDPMGNNITFSGLANATDSAPTSKDAFLEATSEDEIISRASSDASDLLRQTLGSSQKKKKIAVMTSGGDSPGMNAAVRAVVRTGIHFGCDVFAVYEGYEGLLRGGKYLKKMAWEDVRGWLSEGGTLIGTARSMEFRKREGRRQAAGNLISQGIDALVVCGGDGSLTGADLFRHEWPSLVDELVAEGRFTKEEVAPYKNLSIVGLVGSIDNDMSGTDSTIGAYSALERICEMVDYIDATAKSHSRAFVVEVMGRHCGWLALMAGIATGADYIFIPERAVPHGKWQDELKEVCQRHRSKGRRNNTIIVAEGALDDQLNPVTANDVKDALIELGLDTKVTILGHVQRGGTAVAHDRWLATLQGVDAVKAVLEFTPETPSPLIGILENKIIRMPLVESVKLTKSVATAIENKDFDKAISLRDTEFIELYENFLSTTVKDDGSELLPVSDRLNIGIVHVGAPSAALNAATRAATLYCLSHGHKPYAIMNGFSGLIQTGEVKELSWIDVENWHNLGGSEIGTNRSVASEDLGTIAYYFQKNKLDGLIILGGFEGFRSLKQLRDGRTQHPIFNIPMCLIPATVSNNVPGTEYSLGVDTCLNALVNYTDDIKQSASATRRRVFVCEVQGGHSGYIASFTGLITGAVSVYTPEKKIDLASIREDITLLKENFRHDKGENRNGKLLVRNEQASSVYSTQLLADIISEASKGKFGVRTAIPGHVQQGGVPSSKDRVTASRFAVKCIKFIEQWNKKNEASPNTDAKVLRFKFDTHGEKVPTVEHEDDSAAVICVNGSHVSFKPIANLWENETNVELRKGFEVHWAEYNKIGDILSGRLKLRAEVAALAAENK

Target ORF information:

RefSeq Version	NM_001181369.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C 6-phosphofructokinase subunit alpha (PFK1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181369.1

ORF Insert Sequence:

ATGCAATCTC AAGATTCATG CTACGGTGTT GCATTCAGAT CTATCATCAC AAATGATGAA 
GCTTTATTCA AGAAGACCAT TCACTTTTAT CACACTCTAG GATTTGCAAC TGTGAAAGAT 
TTCAACAAAT TCAAACATGG TGAAAATAGC TTACTATCTT CAGGGACTTC CCAAGATTCC 
TTGAGAGAAG TTTGGTTAGA ATCTTTCAAG TTGAGTGAGG TTGATGCTTC TGGGTTCCGT 
ATACCACAAC AAGAAGCTAC TAACAAGGCT CAAAGTCAAG GTGCTCTATT AAAGATTCGT 
TTAGTGATGT CTGCTCCAAT CGATGAAACT TTCGACACCA ACGAAACCGC CACAATCACT 
TATTTCTCTA CTGATTTGAA CAAGATTGTC GAGAAATTCC CAAAACAAGC CGAAAAATTG 
TCCGATACCT TAGTGTTTTT GAAAGATCCA ATGGGCAACA ACATCACCTT CTCAGGCTTA 
GCTAATGCAA CCGATTCCGC TCCAACTTCC AAAGATGCTT TCTTAGAAGC TACCTCCGAA 
GACGAAATCA TCTCTAGAGC TTCTTCCGAT GCTTCTGACT TACTAAGACA AACATTGGGC 
TCTTCTCAAA AGAAGAAGAA GATTGCTGTC ATGACTTCTG GTGGTGATTC TCCAGGTATG 
AATGCCGCTG TTCGTGCCGT TGTTCGTACA GGTATACATT TCGGCTGTGA TGTTTTTGCT 
GTTTACGAAG GTTACGAAGG TTTACTAAGA GGCGGTAAAT ATTTAAAGAA AATGGCTTGG 
GAAGATGTCA GAGGTTGGTT AAGTGAAGGT GGTACTTTGA TTGGTACTGC TCGTTCTATG 
GAATTCAGAA AGCGTGAGGG TCGTAGACAA GCTGCAGGCA ATTTAATTTC GCAAGGTATT 
GACGCTTTGG TTGTTTGTGG TGGTGATGGT TCTTTAACCG GTGCTGATCT TTTCAGACAC 
GAATGGCCAT CTTTGGTTGA TGAATTGGTT GCAGAAGGTA GATTCACTAA AGAAGAAGTC 
GCCCCATACA AGAATTTGTC CATTGTTGGT CTTGTCGGTT CCATCGATAA TGATATGTCT 
GGTACTGACT CTACCATTGG TGCTTATTCT GCTTTGGAAA GAATCTGTGA AATGGTTGAC 
TACATTGATG CCACCGCTAA ATCCCACTCC CGTGCCTTTG TTGTTGAAGT TATGGGTAGA 
CATTGTGGTT GGTTGGCCTT GATGGCTGGT ATTGCTACCG GTGCCGATTA CATTTTTATT 
CCAGAAAGAG CTGTTCCTCA CGGAAAATGG CAGGACGAAT TGAAGGAAGT GTGCCAAAGA 
CACAGAAGTA AGGGTAGAAG AAATAACACA ATTATTGTCG CTGAAGGTGC TTTAGATGAT 
CAATTAAACC CTGTTACTGC CAATGACGTC AAAGATGCTT TGATTGAATT GGGTCTAGAC 
ACCAAGGTAA CCATTCTAGG TCACGTTCAA AGAGGTGGTA CAGCTGTTGC TCATGACAGA 
TGGTTAGCTA CTCTACAAGG TGTCGATGCT GTTAAGGCCG TTCTGGAATT TACCCCTGAA 
ACTCCTTCTC CATTAATTGG TATTTTAGAA AACAAGATAA TTAGAATGCC ATTGGTTGAA 
TCTGTGAAGT TGACTAAATC TGTTGCCACT GCCATTGAAA ACAAAGATTT CGATAAGGCA 
ATTTCTTTAA GAGACACAGA ATTTATTGAA CTTTACGAAA ACTTCTTATC CACTACCGTT 
AAAGATGATG GTTCCGAATT ATTGCCAGTA TCTGACAGAC TAAACATTGG TATTGTCCAT 
GTTGGTGCCC CATCTGCTGC TTTGAACGCT GCCACCCGTG CCGCAACTCT ATACTGTTTG 
TCTCACGGCC ATAAACCATA CGCTATCATG AATGGTTTCA GTGGATTGAT TCAAACCGGT 
GAAGTGAAGG AACTATCATG GATTGATGTC GAAAACTGGC ATAACTTGGG TGGTTCCGAA 
ATCGGTACGA ACAGATCTGT TGCTTCAGAA GATTTGGGTA CCATTGCTTA CTACTTCCAA 
AAGAACAAGC TAGACGGTTT GATTATTCTT GGTGGTTTTG AAGGTTTCAG GTCCTTGAAG 
CAATTGCGTG ACGGTAGAAC CCAACACCCA ATCTTTAACA TTCCAATGTG TTTGATTCCA 
GCCACTGTTT CTAACAACGT TCCAGGTACT GAATACTCAC TTGGTGTTGA TACCTGTTTG 
AACGCATTAG TCAATTACAC TGATGACATC AAACAGAGTG CTTCTGCGAC AAGAAGAAGA 
GTCTTCGTCT GTGAAGTCCA AGGTGGTCAC TCTGGTTACA TCGCTTCTTT CACTGGTTTA 
ATCACTGGTG CTGTTTCAGT GTACACTCCA GAAAAGAAGA TCGACTTAGC TTCTATCAGA 
GAAGATATAA CTCTATTAAA AGAGAACTTT CGTCACGACA AAGGTGAAAA CAGAAACGGT 
AAGCTATTGG TTAGAAACGA ACAAGCTTCT AGCGTATATA GCACTCAATT GTTGGCTGAC 
ATCATCTCTG AAGCAAGCAA GGGTAAGTTT GGTGTTAGAA CTGCTATCCC AGGCCATGTT 
CAACAAGGTG GTGTTCCATC TTCTAAAGAC CGTGTCACCG CTTCCAGATT TGCTGTCAAA 
TGTATCAAGT TTATCGAACA ATGGAACAAG AAAAATGAAG CTTCTCCAAA CACTGACGCT 
AAGGTTTTGA GATTCAAGTT CGATACTCAC GGTGAAAAGG TACCAACTGT TGAGCACGAA 
GATGACTCTG CTGCTGTTAT CTGTGTTAAT GGTTCTCACG TTTCCTTCAA GCCAATTGCT 
AACCTTTGGG AAAACGAAAC CAACGTTGAA TTAAGAAAGG GTTTTGAAGT TCACTGGGCT 
GAATACAACA AGATTGGTGA CATCCTGTCC GGTAGATTAA AGTTGAGAGC TGAGGTAGCC 
GCTTTAGCCG CTGAAAACAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PFK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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