RTG3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RTG3
Entrez Gene ID	852171
Full Name	Rtg3p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178343.1	NP_009447.1	Rtg3p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RTG3	NP_009447.1
Kluyveromyces lactis	KLLA0E06513g	XP_454241.1
Eremothecium gossypii	AGOS_ADR067W	NP_984163.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the function of Aup1 in mitophagy could be explained through its regulation of Rtg3-dependent transcription.
Title: Aup1-mediated regulation of Rtg3 during mitophagy.

Rtg1/3p, Gln3p, and Gat1p can be differentially regulated through different nutrient-sensing pathways, such as TOR and retrograde signaling, and by multiple factors, such as Lst8p, which is suggested to have a role in connecting the RTG and TOR pathways
Title: Retrograde response to mitochondrial dysfunction is separable from TOR1/2 regulation of retrograde gene expression.

Tor signaling and Mks1p phosphorylation regulate expression of Rtg1.Rtg3p-dependent target genes
Title: Tor signaling and nutrient-based signals converge on Mks1p phosphorylation to regulate expression of Rtg1.Rtg3p-dependent target genes.

The following RTG3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RTG3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01613	NM_001178343.1 Latest version!	Saccharomyces cerevisiae S288C Rtg3p (RTG3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RTG3 ( NM_001178343.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009447.1 Saccharomyces cerevisiae S288C Rtg3p (RTG3), partial mRNA.

Price & Availability↑

CloneID	OSi01613
Clone ID Related Accession (Same CDS sequence)	NM_001178343.1
Accession Version	NM_001178343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Rtg3p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATGAACA ATAACGAAAG TGAGGCTGAG AACCAACGTC TACTGGACGA ATTAATGAAC 
CAGACAAAAG TCCTCCAGGA AACTTTAGAT TTTTCGTTAG TGACACCCAC TCCACACCAC 
AATGATGATT ACAAGATACA CGGAAGTGCC TACCCAGGTG GTGAGACTCC TGCCCAACAG 
CATGAAAAAC TCTCATACAT CAATACGCAC AACTCTAACG ATAATAATAA CTTAATGGGC 
AGTCAAGCGA GGTCCAATTC ACAAACTCCT ACAGCTTCGA CCATATATGA GGAAGCAGAA 
TCGCAATCGT CTTACCTGGA TGATATGTTT AGAACAAGCC AAGGCGGTAG ACCTGTCACT 
CAAAATTCCA TATCTTCCAT AGGGCAGGGT CCCTTGAGAT CATCTTATTC TATGGCTTAC 
GACTCACCTG TGGATAGAGC AATGAATACT CCATTACAGC AACAAGAAGG CTTAAAAGCT 
GAGTTACCAC ATGACTTTTT ATTTCAGCAT GGCACCGATG ACACAATGTA TAACTTAACT 
GATGATTTGA GCTCCTCTTT ATCTTCTAGC ATCAATTCTG ATATGATGAC ACCAAACACA 
TATTCATCAT CATTTTCTTA TAATCCACAA AGCTTGGGCC CAGCATCCGT ATCTTCCACA 
TATTCCCCAA AGGTAAGATC ACCATCATCG TCATTTCGTG CAGGAAGTTT CCTTTCATCT 
TCTTTTAGGC ATGGTAGCAT AAACACACCC AGAACAAGAC ACACTTCAAT AAGTAGCAAT 
ATGACTGAAA ATATAGGACC TGGAAGTGTT CCAAAGATTT TAGGCGGGTT AACTTCTGAT 
GAGAAACTGA GGCGCAAAAG AGAGTTTCAT AATGCCGTCG AAAGGAGAAG AAGAGAATTA 
ATAAAGCAAA AGATAAAAGA ACTCGGCCAG TTGGTTCCAC CATCTTTATT GAATTACGAT 
GACCTAGGTA AACAAATCAA ACCAAATAAG GGTATCATTT TGGATAGAAC GGTCGAATAT 
TTACAATACC TGGCCGAAAT TTTAGAAATA CAAGCACGAA AAAAGAAGGC GTTATTGGCG 
AAGATAAAAG AATTGGAAGA GAAGAAAAGT TCTGTCGCAG CATTATCTCC TTTTACCAAT 
AACCACCATG CCAGTTCGGG GCAAAATAAT AGCGAGAATA GTGAGGAAAG AATTATAGAT 
ATTAGATCTG TCCCAAATGC TTTGATGAAT GAACAAAATA GTAAGGCTGA ATTGCACAAT 
TGGGAGCCAC CGTTATATGA TTCGGTCGGT AACCACAATC ATGCTGGCAC TATGGAGAGT 
CATCCACATA CAAACATTCA TGAAGAATTA AAGGAGTTCT TATCAGGCGA TTTGATTGAA 
GCCGAAGATA ATGCAAAATT AATGTTTGGA GATGACAACT CTAATCCAGC TGACTATCTT 
TTAGAATTTG GTTCGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009447.1
CDS	1..1461
Translation	MMNNNESEAENQRLLDELMNQTKVLQETLDFSLVTPTPHHNDDYKIHGSAYPGGETPAQQHEKLSYINTHNSNDNNNLMGSQARSNSQTPTASTIYEEAESQSSYLDDMFRTSQGGRPVTQNSISSIGQGPLRSSYSMAYDSPVDRAMNTPLQQQEGLKAELPHDFLFQHGTDDTMYNLTDDLSSSLSSSINSDMMTPNTYSSSFSYNPQSLGPASVSSTYSPKVRSPSSSFRAGSFLSSSFRHGSINTPRTRHTSISSNMTENIGPGSVPKILGGLTSDEKLRRKREFHNAVERRRRELIKQKIKELGQLVPPSLLNYDDLGKQIKPNKGIILDRTVEYLQYLAEILEIQARKKKALLAKIKELEEKKSSVAALSPFTNNHHASSGQNNSENSEERIIDIRSVPNALMNEQNSKAELHNWEPPLYDSVGNHNHAGTMESHPHTNIHEELKEFLSGDLIEAEDNAKLMFGDDNSNPADYLLEFGSG

Target ORF information:

RefSeq Version	NM_001178343.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Rtg3p (RTG3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178343.1

ORF Insert Sequence:

ATGATGAACA ATAACGAAAG TGAGGCTGAG AACCAACGTC TACTGGACGA ATTAATGAAC 
CAGACAAAAG TCCTCCAGGA AACTTTAGAT TTTTCGTTAG TGACACCCAC TCCACACCAC 
AATGATGATT ACAAGATACA CGGAAGTGCC TACCCAGGTG GTGAGACTCC TGCCCAACAG 
CATGAAAAAC TCTCATACAT CAATACGCAC AACTCTAACG ATAATAATAA CTTAATGGGC 
AGTCAAGCGA GGTCCAATTC ACAAACTCCT ACAGCTTCGA CCATATATGA GGAAGCAGAA 
TCGCAATCGT CTTACCTGGA TGATATGTTT AGAACAAGCC AAGGCGGTAG ACCTGTCACT 
CAAAATTCCA TATCTTCCAT AGGGCAGGGT CCCTTGAGAT CATCTTATTC TATGGCTTAC 
GACTCACCTG TGGATAGAGC AATGAATACT CCATTACAGC AACAAGAAGG CTTAAAAGCT 
GAGTTACCAC ATGACTTTTT ATTTCAGCAT GGCACCGATG ACACAATGTA TAACTTAACT 
GATGATTTGA GCTCCTCTTT ATCTTCTAGC ATCAATTCTG ATATGATGAC ACCAAACACA 
TATTCATCAT CATTTTCTTA TAATCCACAA AGCTTGGGCC CAGCATCCGT ATCTTCCACA 
TATTCCCCAA AGGTAAGATC ACCATCATCG TCATTTCGTG CAGGAAGTTT CCTTTCATCT 
TCTTTTAGGC ATGGTAGCAT AAACACACCC AGAACAAGAC ACACTTCAAT AAGTAGCAAT 
ATGACTGAAA ATATAGGACC TGGAAGTGTT CCAAAGATTT TAGGCGGGTT AACTTCTGAT 
GAGAAACTGA GGCGCAAAAG AGAGTTTCAT AATGCCGTCG AAAGGAGAAG AAGAGAATTA 
ATAAAGCAAA AGATAAAAGA ACTCGGCCAG TTGGTTCCAC CATCTTTATT GAATTACGAT 
GACCTAGGTA AACAAATCAA ACCAAATAAG GGTATCATTT TGGATAGAAC GGTCGAATAT 
TTACAATACC TGGCCGAAAT TTTAGAAATA CAAGCACGAA AAAAGAAGGC GTTATTGGCG 
AAGATAAAAG AATTGGAAGA GAAGAAAAGT TCTGTCGCAG CATTATCTCC TTTTACCAAT 
AACCACCATG CCAGTTCGGG GCAAAATAAT AGCGAGAATA GTGAGGAAAG AATTATAGAT 
ATTAGATCTG TCCCAAATGC TTTGATGAAT GAACAAAATA GTAAGGCTGA ATTGCACAAT 
TGGGAGCCAC CGTTATATGA TTCGGTCGGT AACCACAATC ATGCTGGCAC TATGGAGAGT 
CATCCACATA CAAACATTCA TGAAGAATTA AAGGAGTTCT TATCAGGCGA TTTGATTGAA 
GCCGAAGATA ATGCAAAATT AATGTTTGGA GATGACAACT CTAATCCAGC TGACTATCTT 
TTAGAATTTG GTTCGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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RTG3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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