KIN28 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	KIN28
Entrez Gene ID	851450
Full Name	TFIIH complex serine/threonine-protein kinase subunit KIN28
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180167.1	NP_010175.1	TFIIH complex serine/threonine-protein kinase subunit KIN28

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CDK7	XP_001160482.1
Canis lupus familiaris (dog)	CDK7	XP_850424.1
Bos taurus (cattle)	CDK7	NP_001069183.1
Gallus gallus (chicken)	CDK7	XP_001234587.2
Magnaporthe oryzae (rice blast fungus)	MGG_13401	XP_003711393.1
	CDK7	XP_001092242.1
Danio rerio (zebrafish)	cdk7	NP_998126.2
Drosophila melanogaster (fruit fly)	Cdk7	NP_511044.1
Homo sapiens (human)	CDK7	NP_001790.1
Mus musculus (house mouse)	Cdk7	NP_034004.2
Schizosaccharomyces pombe (fission yeast)	mcs6	NP_596349.1
Neurospora crassa	NCU03659	XP_960739.1
Rattus norvegicus (Norway rat)	Cdk7	XP_006231929.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002646	XP_312281.3
Caenorhabditis elegans (roundworm)	cdk-7	NP_490952.2
Saccharomyces cerevisiae (baker's yeast)	KIN28	NP_010175.1
Kluyveromyces lactis	KLLA0E14785g	XP_454616.1
Eremothecium gossypii	AGOS_ADL283W	NP_983813.1
Arabidopsis thaliana (thale cress)	CAK4	NP_176847.1
Xenopus tropicalis (tropical clawed frog)	cdk7	NP_001017219.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inactivating Kin28 impacts promoter release to differing degrees and reveals a "checkpoint" during the transition to productive elongation, resulting in widespread mRNA stabilization.
Title: Engineered Covalent Inactivation of TFIIH-Kinase Reveals an Elongation Checkpoint and Results in Widespread mRNA Stabilization.

Kin28 has a role in the association of Mediator with promoters.
Title: Kin28 regulates the transient association of Mediator with core promoters.

These results identify an Spt5 phosphorylated C-terminal repeats-independent pathway of Paf1C recruitment requiring Kin28, Bur1, and the Spt5 phosphorylated C-terminal repeats-interaction domain of Cdc73.
Title: Pol II CTD kinases Bur1 and Kin28 promote Spt5 CTR-independent recruitment of Paf1 complex.

Study points strongly toward a minor role of TFIIH kinase in Pol II transcription, and a more significant role in mRNA capping in budding yeast.
Title: Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome.

Results implicate the TFIIH kinase in placing bivalent Ser5 and Ser7 marks early in gene transcription.
Title: TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II.

The following KIN28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIN28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00998	NM_001180167.1 Latest version!	Saccharomyces cerevisiae S288C TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIN28 ( NM_001180167.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010175.1 Saccharomyces cerevisiae S288C TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28), partial mRNA.

Price & Availability↑

CloneID	OSi00998
Clone ID Related Accession (Same CDS sequence)	NM_001180167.1
Accession Version	NM_001180167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	TFIIH complex serine/threonine-protein kinase subunit KIN28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAAGTGA ATATGGAGTA CACAAAGGAA AAGAAAGTTG GTGAGGGTAC TTATGCGGTT 
GTTTACTTGG GTTGTCAACA CTCTACTGGA AGAAAGATTG CTATTAAGGA GATCAAAACA 
TCCGAATTTA AAGATGGTTT AGATATGTCA GCTATCCGTG AAGTTAAGTA CCTCCAAGAA 
ATGCAGCATC CGAACGTCAT AGAACTAATA GACATATTTA TGGCTTATGA TAATTTAAAT 
CTCGTTCTGG AGTTCCTACC AACTGATCTA GAGGTGGTAA TAAAAGACAA ATCAATACTG 
TTTACACCAG CAGATATTAA GGCATGGATG CTTATGACTT TGAGGGGCGT GTATCATTGC 
CACAGAAATT TCATTTTGCA CAGGGATCTG AAACCAAACA ATTTATTATT TTCACCTGAT 
GGCCAGATAA AAGTAGCAGA TTTCGGTCTA GCAAGGGCGA TACCGGCCCC ACATGAGATA 
CTGACAAGTA ACGTCGTAAC AAGATGGTAT AGAGCGCCAG AATTGTTGTT TGGAGCTAAA 
CATTACACAT CGGCTATTGA TATCTGGTCA GTAGGCGTTA TATTCGCGGA ATTAATGCTA 
AGGATACCTT ATTTACCAGG ACAGAATGAT GTCGATCAAA TGGAAGTAAC GTTCAGGGCC 
TTAGGGACAC CTACAGATAG AGATTGGCCC GAAGTTTCTT CCTTTATGAC GTATAACAAG 
TTACAAATAT ATCCGCCCCC TTCAAGAGAT GAATTGAGGA AAAGGTTCAT TGCTGCTAGC 
GAATACGCCT TAGATTTTAT GTGTGGAATG CTAACGATGA ACCCACAAAA GAGGTGGACC 
GCTGTTCAGT GTTTAGAAAG TGATTATTTC AAAGAATTAC CACCACCAAG TGACCCGTCT 
TCAATAAAAA TACGTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010175.1
CDS	1..921
Translation	MKVNMEYTKEKKVGEGTYAVVYLGCQHSTGRKIAIKEIKTSEFKDGLDMSAIREVKYLQEMQHPNVIELIDIFMAYDNLNLVLEFLPTDLEVVIKDKSILFTPADIKAWMLMTLRGVYHCHRNFILHRDLKPNNLLFSPDGQIKVADFGLARAIPAPHEILTSNVVTRWYRAPELLFGAKHYTSAIDIWSVGVIFAELMLRIPYLPGQNDVDQMEVTFRALGTPTDRDWPEVSSFMTYNKLQIYPPPSRDELRKRFIAASEYALDFMCGMLTMNPQKRWTAVQCLESDYFKELPPPSDPSSIKIRN

Target ORF information:

RefSeq Version	NM_001180167.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180167.1

ORF Insert Sequence:

ATGAAAGTGA ATATGGAGTA CACAAAGGAA AAGAAAGTTG GTGAGGGTAC TTATGCGGTT 
GTTTACTTGG GTTGTCAACA CTCTACTGGA AGAAAGATTG CTATTAAGGA GATCAAAACA 
TCCGAATTTA AAGATGGTTT AGATATGTCA GCTATCCGTG AAGTTAAGTA CCTCCAAGAA 
ATGCAGCATC CGAACGTCAT AGAACTAATA GACATATTTA TGGCTTATGA TAATTTAAAT 
CTCGTTCTGG AGTTCCTACC AACTGATCTA GAGGTGGTAA TAAAAGACAA ATCAATACTG 
TTTACACCAG CAGATATTAA GGCATGGATG CTTATGACTT TGAGGGGCGT GTATCATTGC 
CACAGAAATT TCATTTTGCA CAGGGATCTG AAACCAAACA ATTTATTATT TTCACCTGAT 
GGCCAGATAA AAGTAGCAGA TTTCGGTCTA GCAAGGGCGA TACCGGCCCC ACATGAGATA 
CTGACAAGTA ACGTCGTAAC AAGATGGTAT AGAGCGCCAG AATTGTTGTT TGGAGCTAAA 
CATTACACAT CGGCTATTGA TATCTGGTCA GTAGGCGTTA TATTCGCGGA ATTAATGCTA 
AGGATACCTT ATTTACCAGG ACAGAATGAT GTCGATCAAA TGGAAGTAAC GTTCAGGGCC 
TTAGGGACAC CTACAGATAG AGATTGGCCC GAAGTTTCTT CCTTTATGAC GTATAACAAG 
TTACAAATAT ATCCGCCCCC TTCAAGAGAT GAATTGAGGA AAAGGTTCAT TGCTGCTAGC 
GAATACGCCT TAGATTTTAT GTGTGGAATG CTAACGATGA ACCCACAAAA GAGGTGGACC 
GCTGTTCAGT GTTTAGAAAG TGATTATTTC AAAGAATTAC CACCACCAAG TGACCCGTCT 
TCAATAAAAA TACGTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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KIN28 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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